LOCUS       DATDAY010000001         2625 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_2644879, whole genome shotgun sequence.
ACCESSION   DATDAY010000001 DATDAY010000000
VERSION     DATDAY010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2625)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2625)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2625
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2644879"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..993
                     /locus_tag="VIS74_00005"
     CDS             <1..993
                     /locus_tag="VIS74_00005"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEY5791645.1"
                     /translation="MADFLAWRRGEKSIPPKAFVITIDDGYNDAYSIAWPILKEFGYP
                     FTYYIYINYVGAGGRSIKWEQLEEMRDAGVDIGSHTISHDNLIRPKAKSLAGMPYDAW
                     LLNELQGSKTALEQRLGIKVMTLAYPYGIHNEKVMQVAAAAGYEAAFTVNGQKALHSS
                     PPQNIGRYIVQSTIPSTFTNSLKFGGSTGPATQVLGNPAAAAMLTEPMQGATISDPKP
                     EIKINLASLGAIDPKTVEMRISGFGLVPATYDPASQLLTYPMHTRLRESEVTVIVSAR
                     VAGRKAVTSWTFHYDPNATPSPTPVVTLPAPPAPASSAAPAADEAGIPAMKPAE"
     gene            1243..1497
                     /locus_tag="VIS74_00010"
     CDS             1243..1497
                     /locus_tag="VIS74_00010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5791646.1"
                     /translation="MGSGIVEFFRRKLGGEPPLHPVDRRMAKHWVKQRLAHIFPELRG
                     NPEALEQAYRELDLEARPGSGRGGETVFEAILPGGRERDW"
     gene            complement(1535..2410)
                     /locus_tag="VIS74_00015"
     CDS             complement(1535..2410)
                     /locus_tag="VIS74_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY5791647.1"
                     /translation="MVSRLILLFCLPAMLMADTPQTPLHLWEKNAPGAKGTQPTDIPT
                     LTPYLPAPEKATGAAIVVCPGGGYFKLAPHEGDGYARWLSGQGIAAFVLKYRLGASGY
                     RHPVMLNDAARAVRTVRANAAKWNVDPQRIGIMGSSAGGHLAATLLTHFDAGNAASTD
                     PVERVSSRPDIGILCYPVITMGPLTHQGSKDNLLGPHPPAELVDELSAEKRVRNDTPP
                     TFIWHTAEDPSVAVENSLLFGMALRAHKVPFDLHIYEKGPHGLGLGKNAFTAGKFHPW
                     TDDLTYFLRGHGFLK"
     gene            complement(2419..>2625)
                     /locus_tag="VIS74_00020"
     CDS             complement(2419..>2625)
                     /locus_tag="VIS74_00020"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: protein motif:HMM:NF013811.4"
                     /note="catalyzes the transfer of phosphate between the C1
                     and C5 carbons of pentose; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HEY5791648.1"
                     /translation="VLITADHGNDPTFRGTDHTRERVPCFLLHGQTTGLLGVRDTFAD
                     VAGTLAAYFHLDAWPRGEILGRLS"
BASE COUNT          488 a          907 c          822 g          408 t
ORIGIN      
        1 atggcggact tcctcgcctg gcggcgcggc gaaaagtcca tcccgcccaa ggccttcgtc
       61 atcacgatcg atgacggcta caacgacgcc tacagcatcg cctggccgat cctcaaggaa
      121 ttcggctacc cgttcaccta ctacatttac atcaattacg tcggcgccgg cggccgctcg
      181 atcaagtggg agcagctcga ggaaatgcgc gacgccgggg tcgatatcgg cagccacacc
      241 atctcgcacg acaacctcat ccgtccaaag gcgaaaagcc tcgccggcat gccctacgac
      301 gcctggctgc tcaacgagct ccagggctcg aagaccgccc tcgagcagag gctcggcatc
      361 aaggtcatga cgctcgccta tccctacggc atccacaacg agaaggtcat gcaagtcgcc
      421 gccgccgccg gctacgaggc ggcattcacc gtgaacggcc agaaggccct gcacagctcc
      481 ccgccgcaaa acatcggccg ctacatcgtc caaagcacca tcccgtccac ctttacgaac
      541 tcgctgaaat tcggcggctc caccggcccg gccacccagg tcctgggcaa ccccgccgcc
      601 gccgccatgc tcaccgagcc gatgcagggc gcgacgattt ccgatccgaa gcccgagatc
      661 aaaatcaacc tcgccagcct cggcgccatc gacccgaaga ccgtggaaat gcgcatcagc
      721 ggcttcggcc tcgtgcccgc gacctacgat cccgcctccc agctcctcac ctacccgatg
      781 cacacccgcc tgcgcgagag cgaggtcacc gtcatcgtca gcgcccgcgt cgccggccgc
      841 aaagccgtca ccagctggac cttccactac gacccaaacg ccacgccgag ccccactccc
      901 gtggtcaccc tgccggcgcc gcccgcgccg gcatcctccg ccgcgcccgc cgccgacgag
      961 gccgggattc ccgcgatgaa gccggcggaa taaggccaaa accaggaaat cactggacgc
     1021 tggagccccc gccgaccaac gcgaaggggt gagcgaagcg aagaaaatgc cacggatgta
     1081 agggatacag gaaccgcaga ttgcgccgat gacaccggcc ccttctcccc tcctacggcg
     1141 agccgcttgc tacggtgcgg ctcaccggga cggttcgccc taccgaaccg tagctggata
     1201 attcctttcc ctccgcccca tttgcggcga atttcccgcg ccatgggctc gggcatcgtc
     1261 gagtttttcc ggcgcaagct gggcggcgag ccgcccctgc atcccgtgga ccgccggatg
     1321 gcgaaacatt gggtgaaaca acggctggcg cacattttcc ccgagctgcg agggaatccc
     1381 gaggcgctcg agcaggccta ccgcgaactg gatctcgagg cgcgccccgg cagcgggcgc
     1441 ggcggcgaga ccgtcttcga ggcgatcctg cccggtggca gggagcgcga ttggtgagcc
     1501 ctccgcacgc cgagcgcccg gcatgcgcgc cgcgtcattt caagaatccg tgcccgcgca
     1561 ggaagtaggt caggtcgtcc gtccagggat ggaattttcc agccgtgaag gcatttttgc
     1621 ccagcccgag accgtgcggc cctttttcgt agatgtgcag gtcgaagggc actttgtgcg
     1681 cgcggagggc catgccaaac agcaggctgt tttccaccgc aacggacgga tcctcggcgg
     1741 tgtgccagat gaatgtcggc ggcgtgtcgt tgcggacccg cttctccgcc gacagctcat
     1801 cgacgagttc ggccggggga tggggaccga gcaggttgtc cttcgacccc tggtgagtca
     1861 acgggcccat ggtgatcacg ggatagcaga ggatgccgat atccggccgg ctgctcaccc
     1921 gctcgacggg gtccgtcgag gcggcgttgc ccgcgtcgaa atgggtgagc agcgtcgccg
     1981 ccagatgccc gcccgcggaa gagcccatga tgccgatgcg ctgcggatcg acgttccatt
     2041 ttgccgcatt ggcgcggacg gtgcgcaccg ccctggccgc gtcgttgagc atgaccgggt
     2101 gccggtaacc gctcgccccg aggcggtatt ttaaaacgaa tgcggcgatt ccctgcccgg
     2161 aaagccagcg ggcataaccg tcgccctcgt ggggtgcgag tttgaaatag ccgccaccgg
     2221 ggcagaccac gatcgcggcg ccggtggctt tctccggcgc gggcagatag ggcgtgaggg
     2281 tcgggatgtc ggtgggctgg gttccctttg cgcccggagc gtttttttcc caaaggtgga
     2341 ggggcgtctg cggcgtgtcg gccatcaaca tggccggaag acagaaaagc aggatcaggc
     2401 gggaaaccat cgggaacatc aggaaaggcg ccccaatatc tctccgcggg gccacgcgtc
     2461 gagatggaaa tacgccgcca gcgtcccggc cacatcggca aacgtgtcgc gcacgcccag
     2521 cagtcccgtg gtttgcccgt gcagcagaaa acagggcaca cgctcgcgcg tgtggtcggt
     2581 gccacggaac gtcgggtcgt tgccgtggtc ggcggtgatg agcac
//
LOCUS       DATDAY010000002         2729 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_2643240, whole genome shotgun sequence.
ACCESSION   DATDAY010000002 DATDAY010000000
VERSION     DATDAY010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2729)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2729)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2729
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2643240"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(4..480)
                     /gene="ssb"
                     /locus_tag="VIS74_00025"
     CDS             complement(4..480)
                     /gene="ssb"
                     /locus_tag="VIS74_00025"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEY5791649.1"
                     /translation="MANLNKVQLIGNLTRDPEVKYTPKGMAVTELGMAINRYYTPDGG
                     ERREETTFIDITLWGKQAELAGQYLKKGRPVYIEGRLQMDTWEDKQSGQKRSKLRVVG
                     ENMQFLDSRRGGGESEEGGYEERRPPSASSAPGAASRPPNRGSSAPNEPADDDIPF"
     gene            complement(491..805)
                     /locus_tag="VIS74_00030"
     CDS             complement(491..805)
                     /locus_tag="VIS74_00030"
                     /inference="COORDINATES: protein motif:HMM:NF013419.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEY5791650.1"
                     /translation="MSKKRYETLLALDTRGKEDSAKDIIERLEKELEAEGLEIEQVQR
                     LENRELSYEHAHQKNAYFVNVVFSAEPAIIAKLRAKFKLDAEIGFFQFLSLPAKKEVV
                     AA"
     gene            complement(802..1371)
                     /gene="pth"
                     /locus_tag="VIS74_00035"
     CDS             complement(802..1371)
                     /gene="pth"
                     /locus_tag="VIS74_00035"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF013368.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEY5791651.1"
                     /translation="MSTRLVAGLGNPGKAYAGTRHNAGFLVVEELARRSGTGWQKESA
                     RHAEVARQPWGWLAKPTTFMNDSGRAIQSLAAYYRIQPAEILAVFDDFALPLGQVRLR
                     KSGSSGGHNGLKSLCLHLGTSEIPRLRIGIGSPNRPTHDYVLDTFAPEELPVLERAVI
                     RAADCVEAILTTGLEPAMNQFNSNLQPEP"
     gene            complement(1487..2149)
                     /locus_tag="VIS74_00040"
     CDS             complement(1487..2149)
                     /locus_tag="VIS74_00040"
                     /inference="COORDINATES: protein motif:HMM:NF004139.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HEY5791652.1"
                     /translation="MANQIKLSARPRTEVGSNAAKKVRSRGSIPANIYGKGSEPFHLE
                     INQKAVEQVLAHASGENILVELEIEDQGKTTNRLSLIQEVQHHPVRRDVLHVDFHAVS
                     ATETLEAEVPIEPVGEADGVKNSGGILQQNLRSLAIECLPKDLPDHLNVDVSALKLGD
                     SIHVRDLVLPAGVTAAVDGEVTVFHVSEPTVAPEPVAEVAAAPEVLKEKKAEAAPAAE
                     KK"
     gene            complement(2160..>2729)
                     /gene="prs"
                     /locus_tag="VIS74_00045"
     CDS             complement(2160..>2729)
                     /gene="prs"
                     /locus_tag="VIS74_00045"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEY5791653.1"
                     /translation="MDLHAQQLQGFFDIPVDHLYAKPVLGEYLHSLNLSSLVVVSPDV
                     GGVKMASAYSQALGASLAIVTKRRKSATEVEALHVIGDVEGKNVMIVDDITETAGTLT
                     SAAKILRAHGAENIYAGVSHAVLTKLAVERLKGSEIKELITTDSVPPVENAGDRVKVV
                     SIAKLIGEGIKRIHEDESVSSLFDLKITH"
BASE COUNT          492 a          795 c          825 g          617 t
ORIGIN      
        1 ggcttagaac gggatgtcgt cgtccgcggg ctcgttcggc gcggaagatc cgcggttggg
       61 cgggcgggag gccgctccgg gagcggagct tgcggacggc gggcggcgtt cttcatagcc
      121 cccctcctcc gactccccgc ccccccggcg gctgtcgaga aactgcatat tctcgccgac
      181 cacccggagc ttggagcgct tctggccgct ctgcttgtcc tcccaggtat ccatctgaag
      241 gcggccttcg atgtaaaccg ggcggccctt tttcagatac tgcccggcca gctcggcctg
      301 tttgccccag agagtgatat cgatgaaggt cgtctcctcg cggcgttcgc cgccgtccgg
      361 cgtataatac cggttgatcg ccatccccaa ttccgtaacg gccattccct tcggggtgta
      421 tttcacctcc ggatcgcggg tcaggttgcc aatgagctga actttattga ggttggccat
      481 ggcggtggaa ttacgcggcg actacttcct tcttggcggg caggctcagg aattggaaga
      541 acccaatttc cgcgtcgagc ttgaacttgg cgcggagctt ggcgatgatg gccggctcgg
      601 cggagaacac cacgttcaca aaataggcgt tcttctggtg agcgtgctcg tagctcaatt
      661 cacggttttc gaggcgctgg acttgctcga tttcgagccc ttccgcctcc agttcttttt
      721 cgaggcgctc aatgatgtcc ttggcgctgt cttccttgcc gcgggtgtcg agggcaagca
      781 gggtttcgta tcgtttctta ctcatggttc gggttgtagg tttgaattaa attggttcat
      841 ggccggttcg aggccggtgg tgagaatggc ttccacgcaa tccgcagccc gaatgaccgc
      901 ccgttccaaa acgggcagct cttccggagc gaatgtgtcg agaacgtaat catgagtcgg
      961 gcggttcggc gagccaattc cgatccgcag gcggggaatt tcgctcgttc ccaggtgcag
     1021 gcacaaggat ttcaagccat tatgcccgcc ggaactcccc gatttccgca gccgcacctg
     1081 gcccaagggc agcgcaaaat catcaaaaac ggccagtatt tccgccggtt ggattcggta
     1141 atacgccgcc agcgactgaa tcgcccgccc gctgtcgttc atgaaagtcg tgggtttggc
     1201 cagccagccc cacggctgcc gggcgacttc cgcgtgccgg gcgctttcct tttgccatcc
     1261 ggtcccgctg cggcgggcca gctcctcgac cacgaggaac ccggcattat ggcgggttcc
     1321 cgcgtaggcc tttccgggat tccccaagcc ggcgacgagg cgcgttgaca ttctcggacc
     1381 aaacaggatt tacaagatta acgggattga ccggataaga gggagaaaaa ttctcccgtt
     1441 aatccagaaa atcccgttaa tccggtttag ttttttagaa ggaaatttat ttcttctcag
     1501 cggccggagc ggcttccgcc ttcttctcct tcaggacttc gggagcggcc gccacttcgg
     1561 caaccggctc cggagccacg gtcggctcgg aaacgtggaa caccgtcact tcgccgtcca
     1621 ccgcggcggt aacgccggcg ggaagaacga ggtcgcggac gtggatggag tcgcccagct
     1681 tgagggcgga aacgtccacg ttgaggtgat cgggcagatc cttcggcaaa cattcgatgg
     1741 ccaggctgcg caggttctgc tggagaatgc cgccgctgtt tttaacgcca tcggcttcac
     1801 ccacgggttc gatcggcact tcggcttcga gcgtttccgt ggcggaaacg gcgtgaaagt
     1861 ccacgtggag cacatcgcgg cggacgggat gatgctgcac ttcctggatc agcgaaaggc
     1921 ggttggtcgt cttgccctgg tcttcgatct cgagctcgac gaggatgttt tcgccggaag
     1981 cgtgcgcgag cacctgctcg acggctttct ggttgatttc caggtggaac ggttcgctgc
     2041 ccttgccgta gatattggcg ggaatggagc cgcggctgcg cactttcttc gcggcgttgc
     2101 tgccgacttc ggtccgggga cgggcggaga gtttgatttg gttggccata ttaaattggt
     2161 tagtgggtga tcttcagatc aaaaagcgaa cttaccgatt catcttcgtg gatgcgttta
     2221 atgccctcgc cgatgagttt ggcgattgag acgactttca cacggtcacc ggcattttca
     2281 accgggggga cgctatcggt ggtgatcagc tccttgattt cagacccctt taatcgctca
     2341 accgctaatt ttgtcaagac tgcatgcgag acgcccgcat agatattctc cgccccatgg
     2401 gcgcggagaa tcttcgcggc gctggtcaaa gtgcccgccg tttcggtgat gtcatccacg
     2461 atcatgacat tctttccctc cacatcgccg atgacgtgca gggcttccac ctcggtcgca
     2521 ctcttccggc gtttggtcac gatcgccagg ctcgccccga gggcctggga ataggcggag
     2581 gccattttca ccccgcccac gtcgggggaa acgacgacga gcgaggaaag attgagggaa
     2641 tgcaggtatt cccccagcac cggcttcgcg tagaggtgat ccacgggaat gtcaaaaaat
     2701 ccctgcaact gctgggcatg caaatccat
//
LOCUS       DATDAY010000003         2230 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_2640647, whole genome shotgun sequence.
ACCESSION   DATDAY010000003 DATDAY010000000
VERSION     DATDAY010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2230)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2230)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2230
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2640647"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2180)
                     /locus_tag="VIS74_00050"
     CDS             complement(<1..2180)
                     /locus_tag="VIS74_00050"
                     /inference="COORDINATES: protein motif:HMM:NF009948.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional fucokinase/fucose-1-phosphate
                     guanylyltransferase"
                     /protein_id="HEY5791654.1"
                     /translation="MWLLSARAIEVLMRKCGWNGQGFASGLPKFYELYADMGPCLGSQ
                     PRRHDPEIATLSSAVLPLPEARFYHFGTSRQMIESVSALQNASLDQTRHPSSALKPHP
                     DIYVLNSDFLFTQRSPENHDIWVENCALPPNLALTNRNVLTGIPRADWDFAIEPGVCL
                     DFAPLGETEFCVRAYGFDDPFKGALVEATYFGQPFGEWLKRRGLNFEAAALDARMDIQ
                     QAPIFPVLPEDHLDPVFIRWLFAVDPGDRPDCRTRWQNAPRLSAYAISERINLRKLYT
                     QRRALAAKATPRLFANRAVNAFNQIDLAHAAELFAESGADVPSARNGDSPLQIIHEEM
                     FSGAVERSRGTGGETPHETRAFGILRDTLVAAASKRPAAPRRNLLEDQIVWGRSPARL
                     DLAGGWTDTPPYCIKFGGAVVNVAVNLNGQPPVQVFARSIERREIVIRSIDLGAETRV
                     RTLAELREYDRVGGEFSLAKGALALAGFDPHFAGRENSCSLADILEDFGGGLEISLLA
                     AAPKGSGLGTSSILAATLLGTLSEACGLGWELDDIFHRTLILEQLLTTGGGWQDQAGG
                     IYRGLKMVETAPGLEQRPLLRWLPDNLLGPAYANQTILLYYTGITRVAKGILREIVRG
                     MFLNRQKHLHLLDRIECQAHHAFEAIQRDSWPDLCASVRESWELNQALDAGTNPPGVA
                     AILDKIGDYLSAVKLLGAGGGGYLLMFAKDLEAAVRLRAKLAAEP"
BASE COUNT          438 a          653 c          731 g          408 t
ORIGIN      
        1 ggctccgcgg cgagtttggc gcggaggcgc acggcggctt cgagatcctt ggcgaacatg
       61 agaaggtagc ccccgccgcc ggcgcccagt aacttgaccg cggaaagata gtctccgatc
      121 ttgtcgagga tcgcggcgac gcccggcggg ttggttccgg catcgagcgc ctggttaagc
      181 tcccagcttt cccgcacgga ggcgcatagg tctggccacg aatcgcgttg aatggcttca
      241 aaggcgtgat gggcttggca ttcgatgcga tccagaagat ggagatgttt ttggcggttc
      301 aaaaacatgc ctctgacgat ttcccgcagg atgccctttg cgacgcgggt aatgcccgta
      361 taataaagga ggatggtttg attggcatag gccgggccca agagattgtc cggcagccag
      421 cggaggagcg ggcgttgctc aaggccgggg gcggtttcga ccatttttaa tccgcggtag
      481 atgcctccgg cctggtcctg ccagccgccg ccagtggtca gcaattgttc gagaatcagg
      541 gtgcggtgga agatgtcatc gagttcccag ccaaggccgc aggcttcgct caacgtgccg
      601 aggagcgtgg cggcaaggat gctgctcgta ccgagaccgg agcccttggg agcggcggcg
      661 agcagggaga tttccaaccc gccgccaaag tcttccagga tgtccgccag ggagcaggaa
      721 ttctcgcggc cggcgaagtg cggatcgaac cccgcgagag caagcgcccc cttggccagg
      781 gaaaattctc cgccgacgcg gtcgtattcg cgaagttccg caagcgtgcg cacccgcgtc
      841 tccgcgccca aatcgatgga acggatgacg atttccctcc gctcaatcga gcgggcaaag
      901 acctgcacgg gcggctggcc attgaggttg acggcaacgt ttaccaccgc tccgccgaat
      961 tttatgcaat aaggcggcgt atcagtccat ccaccggcca ggtcgaggcg ggccgggctg
     1021 cggccccaga caatctggtc ctcgagaaga ttgcgccgcg gcgccgcagg gcgtttgctt
     1081 gcggccgcaa cgagggtgtc ccgcagaatc ccaaaggccc gcgtttcgtg cggcgtttcg
     1141 ccgcccgtgc cgcggctccg ctccacggcg ccggagaaca tttcttcgtg aatgatctga
     1201 agcggcgaat cgccgttcct tgcgctggga acgtccgcgc ctgattccgc aaagagttcg
     1261 gccgcgtgag ccagatcgat ctgattgaag gcattgaccg cccgattggc gaaaaggcgg
     1321 ggggtcgcct tcgcggccag ggcgcgccgc tgcgtgtaaa gtttccgcag gttgatgcgc
     1381 tcgctaatgg cgtaggccga gagccgcgga gcgttttgcc accgagtccg gcagtccggc
     1441 cgatctccgg gatccacggc aaacagccac cggataaaaa ccggatcgag gtgatcctcg
     1501 gggagcacgg gaaagatcgg cgcctgctgg atatccatgc gggcatcgag ggccgccgcc
     1561 tcaaagttga gcccgcggcg cttgagccat tccccgaagg gttggccaaa atacgtcgct
     1621 tcaaccagcg ctcctttgaa aggatcgtca aagccatagg ctcgcacgca gaattccgtt
     1681 tcacccaaag gcgcgaaatc gaggcaaaca cccggctcga tggcaaaatc ccagtctgcc
     1741 cgggggatgc ccgtgaggac gtttcggttg gtcagagcaa ggttcggtgg caaggcgcaa
     1801 ttttccaccc aaatgtcgtg attctcgggg ctgcgttggg tgaaaagaaa atccgagttg
     1861 agaacataga tgtccggatg cggcttgagg gccgacgacg ggtgccgggt ttggtcgagg
     1921 ctggcattct gcagggccga gacggactcg atcatctgcc ggctggtgcc aaaatgataa
     1981 aatcgagctt ccggaagcgg caggacggcc gagctgaggg tggcgatttc cggatcgtgc
     2041 cgccgcggct ggctcccgag acagggtccc atgtccgcat aaagttcgta aaatttcggc
     2101 aagccggatg cgaatccctg gccattccat ccacatttgc gcatcagcac ctcgatggcg
     2161 cgggcgctca gaagccacat gccagtatcg accaggaaaa agtggtcgcc tgcctgttcc
     2221 cggatttccg
//
LOCUS       DATDAY010000004         6871 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_345930, whole genome shotgun sequence.
ACCESSION   DATDAY010000004 DATDAY010000000
VERSION     DATDAY010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 6871)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6871)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6871
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_345930"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..356
                     /locus_tag="VIS74_00055"
     CDS             <1..356
                     /locus_tag="VIS74_00055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5791655.1"
                     /translation="ASPAQQALEIKEEFIWDDLALLWRNGTSYAVEPHPNGGTPVAVF
                     ERPDTLPTFLLSDILGTTLAAIKPDRVEIIPLTAFGKPVNVLVKPATVSPNPLSSGSG
                     NGVSQTVPPQTTQTN"
     gene            363..1100
                     /locus_tag="VIS74_00060"
     CDS             363..1100
                     /locus_tag="VIS74_00060"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HEY5791656.1"
                     /translation="MKKLLIISLGLMVVVGNMHGNENHKLPGISSQLREYFFTGKPYD
                     ADIGGVIFKYRNYDPSQNRWTSMDSSGFPDGVNNCKFVADPIAMFDFRGLEGKKVAFI
                     YAYTGLSATDNSSGNGAPFIQNLVSVTMDKLQQADNKNPEWKYVSDGDYVSYGSITDP
                     AQLASWSDYDKIYIAAHGVFDANGFTGDVIINGLNYSSGTIMTPKTESFKYCGSNTPT
                     PWNPNGIVQNWELSGEWFHSAADYLYE"
     gene            1106..1522
                     /locus_tag="VIS74_00065"
     CDS             1106..1522
                     /locus_tag="VIS74_00065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5791657.1"
                     /translation="MKEVFLFIICLFIPLLEGAFAEQIATFEDSVAKAIPISKIEIKL
                     SGNKYSYLVNGKLAIYRDLAVKQLADSIFKFGDDMPLALIVPSSIPAEELLFWSQTAK
                     KLAVPAMRIFVSDNSKEMLNKPDFGKSVSFDIITTD"
     gene            1637..2266
                     /locus_tag="VIS74_00070"
     CDS             1637..2266
                     /locus_tag="VIS74_00070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5791658.1"
                     /translation="MGKAKRHPLVYLLLSIGIVGTNAIGEERVSEEVMRADRALQQRI
                     AELYRDGRNDYHAGNLTAAERELGECFSLYQQSLPYLLHPEKVAKVAGECEQTLRRVR
                     SLQEFEAKAGGWRKRLQVLAFAQFEIRDADLSATMDYFQQRVGKAFGHTPPNFLYRLA
                     PDKLKSKLTLELRNVTALEVLAAIESQMRIETKFEPHALVFVEAEEVEK"
     gene            2527..3309
                     /locus_tag="VIS74_00075"
     CDS             2527..3309
                     /locus_tag="VIS74_00075"
                     /inference="COORDINATES: protein motif:HMM:NF021982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HEY5791659.1"
                     /translation="METLSPLNPCVVFDGYAPPSLRRLPEKSAVLLLVPGCNQSGKTA
                     FLAPGSEWIPFADRENLVLLTPSFRTSPEELRRREGYYYPARWSGEAAEEALRQLAAR
                     YSVDARRIALFGFSAGAHFAHRFAEWKPDRVVAFAAYSAAWWDEPTDHLARVPALIMC
                     GEDDVRFEATLAYFQRGNALGHPWLWRAYRNTGHEAAAEAMEMARVFLRHYLRKGAEG
                     PQIGDIQTYKVYPMEQLEDIPASCRVILPSAQVAAAWTRERR"
     gene            3318..4166
                     /locus_tag="VIS74_00080"
     CDS             3318..4166
                     /locus_tag="VIS74_00080"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HEY5791660.1"
                     /translation="MILNHKKSNMKIFTIYACLMACVSSIWQIHANNDLPGIPVQSEE
                     CFFTGKPYDADIGGVIFKYRNYDPEISRWTVVDPSGFPDGVNNQLYTSNPLRKFDPLG
                     LTTIDSFNQAVDYWRRSNGSTQDTATIGSSIIGDIKASGGFSDLVQRIQDNRIRRQIN
                     DVDPNDSQGWLPDNPGAGPASIGFVDLVLGRTSISYSDSSSWTAISNWYKNADGVWGR
                     NIRATTNMSITSNEIWDFQAHEGDPGWVNFFEETLPGMLVGAGTPFNLSGQFGFEFEI
                     VGFQTE"
     gene            4167..4586
                     /locus_tag="VIS74_00085"
     CDS             4167..4586
                     /locus_tag="VIS74_00085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5791661.1"
                     /translation="MKKQSIVLYFAVIAFFAVTACQKSEADYQKYFENIFLIKIPKGA
                     YEIKLEESRWFDELLDRHVIFEFQLSGEGLADFIRNPPHPYPQWTKLREVYVAQRLFS
                     YATMPDAQMTKLEKNGDYKILIADPADNKVYALYWSD"
     gene            4698..5744
                     /locus_tag="VIS74_00090"
     CDS             4698..5744
                     /locus_tag="VIS74_00090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5791662.1"
                     /translation="MDAANAQAWPTTQKERLEINRMEREREKAEKKAQQEREKKRAEA
                     AAKPKPAPTPEEMPSVWDVIEVASPDPLNPKVPFYIRPPAGYRASELNQMHRVLVLLT
                     FYQESGIKVLKRSAEYQQIADERGWFMIAPTFRMNRDDARNRAKSYYYPETWSGKAVL
                     QALDEIAKRYPVDGHRLFVSGLSGGAQFAHRFALWAADRVEAAAVNSCGWFDEPGPSA
                     RKVAWVITVGESDPILPESLDFTEKLRAQGATPVLRTYLGMLHEDAPRVNAFCGRFLA
                     ERDDATREFLGKSVEEPAGWKYNVPASHPFVGDRRDGIYYANGSGTLEVIPEQSRIFL
                     PSGAMAELWGQSAE"
     gene            5750..>6871
                     /locus_tag="VIS74_00095"
     CDS             5750..>6871
                     /locus_tag="VIS74_00095"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEY5791663.1"
                     /translation="MEYYYLDRLRWNLGFENPNHAAALIASLLPFLWAVRSLWAPRRK
                     AAAFLWNLLAGGILIAGWWLLAMTLSRGGLVAAIGGFAFLGWKTFAGGKKEWIRASVI
                     AAGIIAAVAFTGLASRTAEGMARPDASIENRFEVWKGGLRMLAIAPGGVGAGESGEFY
                     MHWLQPVDSTAGYRTLVNGYLTWLTEYGLLAGFLVLAAALFVLFGLRPAAAGERSRCW
                     IRASQASLISFALAAVFSTTFETGWVWAPPVMALLILGALTAKHGSVARIPLFRRVSF
                     SLLGSAAAMLLLWGTGAAIESLNPEAIRIGRDGLISVIPKGAVGGWAILPDEEVAGPD
                     YGKRLREIAKEGKIKLIAVPSREAIPECQGIIAMGERVEE"
BASE COUNT         1580 a         1684 c         2011 g         1596 t
ORIGIN      
        1 aagcctcccc ggcccagcaa gcgctggaga taaaggagga attcatctgg gatgatctcg
       61 cgctgctctg gcggaatggg acaagctacg cggtggagcc gcatccgaac ggaggtacgc
      121 cggtcgcggt ttttgaacgg cccgacacgc tgcccacctt tttgttgagc gatattttgg
      181 ggaccaccct cgcggcgata aagccggacc gggtggaaat catcccgctt accgccttcg
      241 gaaaaccagt gaacgtcctg gttaagccag cgaccgtttc acccaaccca ctttcgagcg
      301 gctccggcaa cggggtaagc caaaccgttc cacctcagac aacacaaact aattaataaa
      361 atatgaaaaa attactgatt atctcacttg gactgatggt cgtcgttggg aatatgcatg
      421 gaaacgagaa tcataaattg ccaggaatat cctcgcagct gagggaatat ttttttactg
      481 ggaagccata tgatgctgat ataggtggag tcatttttaa atatcgcaat tacgatccca
      541 gtcaaaaccg ttggacatct atggattcga gtggatttcc tgacggagtt aataattgca
      601 agtttgtagc cgatccaatt gcgatgtttg attttcgagg actagaaggc aaaaaagtag
      661 catttattta cgcatatact ggcttgagtg cgactgataa cagcagtggt aatggggcac
      721 cgtttattca aaatcttgta agtgttacca tggataaact tcaacaagct gacaataaaa
      781 atccagaatg gaaatacgtg agcgatggtg attatgtttc gtacggatca attactgatc
      841 cggcacaact tgctagttgg tctgattacg ataagatcta catagcggcg catggagtgt
      901 ttgacgcgaa tgggtttact ggtgatgtaa taatcaatgg attaaattac agttcaggga
      961 caataatgac accaaagacg gaaagtttta aatattgtgg ttcaaatacg cctaccccgt
     1021 ggaatcctaa cgggattgtt cagaactggg aattatcagg cgaatggttt catagtgcag
     1081 cagattacct ttacgaataa atataatgaa ggaagtattt ttatttataa tatgtttatt
     1141 tattccatta ttggaaggag catttgctga gcaaattgcg acatttgagg attcggtcgc
     1201 gaaggcaatt cccatttcaa aaatcgaaat aaaattaagt ggaaataaat attcttactt
     1261 ggtaaatggc aagttagcta tatacagaga tcttgccgtc aagcaattgg ctgattccat
     1321 ttttaaattc ggtgatgata tgcctcttgc attgatagtc ccatcttcaa ttccagctga
     1381 ggagctttta ttctggtctc agaccgcaaa aaagcttgca gttccagcta tgcggatatt
     1441 tgtgagcgat aactcaaaag aaatgttaaa taagccggat tttggaaaaa gtgtttcttt
     1501 tgatattata accacagact aaaaaacgtt aatgtaggat acaagataga actgcgtctt
     1561 aatatttagc ttcaagaatt cgatgccaaa aattgttttg gggaggaatg attataagac
     1621 tgcgagacgc cttgtcatgg gcaaggctaa acgtcatccg ctggtatatc tgctgctcag
     1681 tatcgggatt gtcggcacga atgccatcgg ggaggagcgc gtatccgaag aagtgatgag
     1741 ggcggatcga gcacttcagc agcggatcgc ggagctttac cgggacgggc gcaatgatta
     1801 ccatgcggga aatctcactg cggcggaaag ggagttaggc gagtgctttt cactttatca
     1861 gcagtcgctg ccctacctcc tgcacccgga gaaagtcgcc aaggtcgccg gagaatgcga
     1921 acaaaccctg cggcgggtga gaagcctgca ggaattcgag gcgaaggcgg gcggctggcg
     1981 gaagcggctc caggtgctcg cgtttgccca gtttgaaatc cgcgacgcgg atctttcggc
     2041 gacgatggac tatttccagc agcgggtggg aaaggccttt ggccacacgc cgcccaattt
     2101 tctctatcgc ctcgcgccgg ataaactgaa gtccaaactc acgctggaat tgcgcaacgt
     2161 gaccgccctg gaggtgctgg ccgcgatcga aagccagatg cggatcgaaa ccaagtttga
     2221 acctcatgcc ctcgtttttg tggaggcgga ggaggtggag aaatgaatcc gcttccccaa
     2281 tcggaaaagt cggatcggcg gctccactgc gtctcccggg aatttttgga gggcgggctg
     2341 ccgcgccggc ccattttcct ccgcttcatc catagaaact tttcgattgg aatggccgca
     2401 tctcgcatcc cgtcctggag ggcgccgctc cgccggcgcc tcgggtggat tcatggggca
     2461 ttccgagcgg ctataatcct ctgtctgttc tcgctttcgg cgggcgcgga gcttttcaag
     2521 atcgaggtgg aaacgctttc gcccctgaat ccatgcgtgg ttttcgacgg ctatgcgccg
     2581 ccgtccctcc gccgcttgcc ggagaaaagc gcggttctgc ttctggttcc gggctgcaac
     2641 caatccggca agacggcatt cctcgctccc ggatcggagt ggatcccatt cgccgaccgg
     2701 gaaaatctgg ttctgctgac cccttccttc cggaccagtc cggaagagct gaggcggcgg
     2761 gagggctatt attatcccgc ccgctggagc ggggaggcgg ccgaggaggc tttgcggcag
     2821 ctggccgcgc gttattccgt ggatgcccgg cggatcgcgc tgtttggctt ttccgccggg
     2881 gcgcatttcg cgcatcgctt tgccgagtgg aagccggatc gggttgtggc cttcgccgct
     2941 tattccgcgg cttggtggga cgagcccacg gaccatctgg cccgggtgcc cgccctcatc
     3001 atgtgcggag aagacgatgt gcgtttcgaa gcgaccctgg cctatttcca gcggggaaac
     3061 gccttggggc atccctggct gtggcgcgcg tatcgaaaca ccggccatga agccgccgcc
     3121 gaagccatgg aaatggcgcg ggtttttttg cggcattacc tgcggaaagg cgccgaggga
     3181 ccgcagatcg gagacattca aacgtataag gtatatccga tggagcaact cgaagacatc
     3241 ccggcatcct gccgcgtgat tctgccttcc gcccaagtgg cggcggcttg gacaagggaa
     3301 agaagataag ctttcaaatg attctcaacc acaaaaaatc aaacatgaaa atattcacaa
     3361 tctacgcatg tctaatggcc tgtgtgtcct caatttggca aattcacgca aacaatgatc
     3421 tgcccggaat tccggttcaa tccgaggaat gcttctttac tggcaagccc tacgatgctg
     3481 acatcggagg cgttatattc aaatatcgaa attatgatcc ggagataagt cggtggactg
     3541 tcgtggatcc aagcggattt ccggatggcg ttaataatca actgtacacc tcaaatcctt
     3601 tgcgaaagtt tgatcctctt ggattaacca ctattgatag ctttaatcaa gctgttgatt
     3661 actggaggcg aagcaacgga agcacgcaag atacggcgac cattggttct tcaattatcg
     3721 gcgatattaa agcatccgga gggttttctg atcttgttca gcgtattcaa gataatcgaa
     3781 tacggcgcca aattaatgat gttgatccaa atgactcgca agggtggctg cctgataatc
     3841 caggtgcggg tcctgctagc attggattcg tggatttggt cttgggacgc acatcaataa
     3901 gctattctga ctcatcctct tggacggcaa ttagtaattg gtataaaaat gcggatggag
     3961 tttgggggcg caacatacgt gcgacaacca atatgagcat tacaagcaat gaaatatggg
     4021 actttcaggc gcatgaaggc gatcctggct gggtgaactt ttttgaagaa acgctgccgg
     4081 ggatgttggt tggtgccgga acccccttta atttaagcgg ccagtttggt tttgaatttg
     4141 aaatagtggg gtttcaaaca gagtaaatga aaaagcaatc tatagttttg tattttgctg
     4201 taattgcatt ttttgcggta acggcctgcc agaaatcgga agcggattat caaaaatatt
     4261 ttgaaaatat ctttttgatt aaaattccca aaggagctta tgaaattaag ttggaggaat
     4321 cccgatggtt tgacgaactg ttggaccggc atgtgatatt tgagttccaa ctttcgggtg
     4381 agggattggc tgatttcatc cgcaatccac cccaccccta tcctcaatgg accaagttaa
     4441 gagaggtgta tgtggcccaa aggttatttt catatgcaac gatgcccgat gcgcaaatga
     4501 cgaagctcga aaaaaatggt gattataaaa ttctgatcgc agaccctgcc gacaacaaag
     4561 tgtatgcgct ttattggagc gattagtcaa aggcgggaat tgagataatt tgtatgaggt
     4621 cgaagaaaca atttcgacga atgtccggca ggcattttct tctgccgatt ttgattgggc
     4681 taattctcta tgtgaaaatg gacgcagcaa acgcccaggc ttggccaacc acgcagaagg
     4741 agcgcctgga gatcaatcgg atggagcggg agcgggaaaa ggccgagaag aaggcgcaac
     4801 aggaacggga gaaaaaacgg gccgaggcgg ccgccaaacc caagcccgcc ccgactccgg
     4861 aggagatgcc ttccgtctgg gacgtgatcg aggttgcctc gccggatcca ttgaacccga
     4921 aggtgccttt ctacatccgg cccccggcgg gttatcgagc ttccgaactg aatcaaatgc
     4981 accgggtgct ggtgttgctg acgttttacc aggaatccgg aatcaaggtt ctgaaacgaa
     5041 gcgccgaata ccagcagatc gccgacgagc gcggctggtt catgattgcg cccacgttcc
     5101 gcatgaaccg ggacgatgcc cggaatcggg ccaaaagtta ttattatccg gaaacctgga
     5161 gcggaaaagc cgtcctccag gccctggacg agatcgccaa acgttatccg gtggacggcc
     5221 accggctgtt cgtttccggc ctgtcgggcg gggcgcagtt cgcccaccgt ttcgcgctgt
     5281 gggcggcgga tcgggtggag gcggcggccg tcaattcctg cggctggttt gacgagcccg
     5341 gccccagcgc ccgcaaggtc gcgtgggtga tcaccgtcgg ggagtccgat cccattttgc
     5401 ccgagagcct ggatttcacg gagaagctgc gggcgcaggg agccacgccc gtgctgcgga
     5461 cctacctggg catgctccat gaggatgcgc cgcgggtgaa cgcgttttgc ggacgctttt
     5521 tggcggaacg ggatgatgcc acccgggaat ttctcgggaa gtccgtggag gaaccggccg
     5581 gttggaaata caatgtcccc gcgagccatc ccttcgtggg ggatcggcgg gacgggattt
     5641 attatgccaa tggttccggc acgctggagg tgatcccgga gcagtcgaga atcttcctgc
     5701 caagcggcgc gatggcggaa ttgtgggggc aatccgcgga gtaaccgcca tggagtatta
     5761 ttacctcgac cggctccgct ggaatctggg ttttgaaaat cccaatcacg cggcggcgtt
     5821 gatcgcctcc cttctgcctt tcctttgggc ggttcggagc ttatgggcgc cgcggcgaaa
     5881 ggcggccgct tttctgtgga acctcctggc cggcggcatc ctcatcgccg gctggtggct
     5941 tctggccatg accttgtcgc gcggcgggtt ggttgcggcc atcggcggat tcgccttcct
     6001 cggatggaaa accttcgccg gcgggaaaaa ggaatggatt cgcgcttcgg tgatcgcggc
     6061 gggaatcatc gccgccgtgg cattcacggg actggcgtcc cgaacggccg agggcatggc
     6121 gcggcccgac gcctcgatcg aaaaccggtt cgaggtctgg aagggcggcc tgcggatgct
     6181 ggccatcgcg ccggggggag tcggcgcggg ggaatcgggc gagttttaca tgcactggct
     6241 ccagccggtg gattccaccg ccggctatcg caccctggtc aacggctacc tgacctggct
     6301 cacggaatac gggctgctgg cgggctttct ggtgctggcg gcggcccttt ttgtcctgtt
     6361 cggcctgcgg ccggcggccg cgggcgagcg gtcccgctgc tggattcggg caagccaggc
     6421 gtcattgatc agctttgccc tcgcggccgt cttcagcacc acctttgaaa ccgggtgggt
     6481 ctgggcgccg ccggtgatgg cgctgctcat tcttggcgcg ctgacggcga agcacggctc
     6541 ggttgcccga attccgcttt tccgccgggt gtccttttcc ctccttggat cggcggcggc
     6601 gatgcttctg ctttggggaa cgggagcggc gattgaatcc ctgaaccccg aggcgattcg
     6661 gattgggagg gacggcttga tctccgtcat ccccaaggga gcggttggag ggtgggcgat
     6721 tctgccggac gaggaagtgg ccggacccga ctatggaaag cggttgcggg aaatcgcgaa
     6781 agaaggaaaa atcaagttga tcgccgtgcc ctcgcgggag gcgattccgg aatgccaggg
     6841 gattatcgcg atgggagaac gagtggagga g
//
LOCUS       DATDAY010000005         2957 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_342539, whole genome shotgun sequence.
ACCESSION   DATDAY010000005 DATDAY010000000
VERSION     DATDAY010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2957)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2957)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2957
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_342539"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..489)
                     /locus_tag="VIS74_00100"
     CDS             complement(<1..489)
                     /locus_tag="VIS74_00100"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF013345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEY5791664.1"
                     /translation="MKFATDAPAPPRTWTEISAAAIEWNARIVRKYCPDAGIIAVLKA
                     DAYGHGAITAARALARSAEYFAVANLNEARVLRQGVPGRPILILSPALPEERPAIAAA
                     GFIPTVSRYEEIAAYAALAGRTPLPVNLKIDTGMGRLGILLQEAAGLLQAARRLPGVE
                     IRH"
     gene            complement(517..732)
                     /locus_tag="VIS74_00105"
     CDS             complement(517..732)
                     /locus_tag="VIS74_00105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5791665.1"
                     /translation="MTASISTNENPRGEFLEETPPDALSFFIPKNKISYFHANLSSQW
                     QPRRCDNLFAFSRSASPPKPPETGVAV"
     gene            719..1342
                     /locus_tag="VIS74_00110"
     CDS             719..1342
                     /locus_tag="VIS74_00110"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5791666.1"
                     /translation="MLAVMAIVTVLLAMLAPAVRSFSDTAGRRGAVNVVMNTLEQARV
                     SALESGREVQVLFYRVAFPHEDRLVVIRELEDGSGYEQLTKWLKLPKGVLLFKPTAGH
                     SVFESAVPASVLTALPANLSTSALGNAGIRVVNGIAAIKFNASGQVAYPTDSSQLLLH
                     LSEGVRDEDGVEARITQKQAASLPFEIISLSKYTGRAQLDITELAAN"
     gene            1404..1892
                     /locus_tag="VIS74_00115"
     CDS             1404..1892
                     /locus_tag="VIS74_00115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5791667.1"
                     /translation="MEITLALGIVAFALVAIMGLFPVAMKNALESQNETRATFIAQSI
                     FSDLESGPASTNTFLAAGTNWSSPGDRVIVNLKAANTFYVKYSDEGAPVGTYSNVSAF
                     EAAGGDAAYGARVIITPDPVLANLSRAEVQVESPLSAASASRAKFNFVTLLPNNLVNT
                     NQ"
     gene            1889..2722
                     /locus_tag="VIS74_00120"
     CDS             1889..2722
                     /locus_tag="VIS74_00120"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEY5791668.1"
                     /translation="MSAKGKNPAVVRGIGILPVRGAGWIIGRMPMPPFGRRRAFTLLE
                     LLVAVSLLAILVVMLMSMVDNATKLWRVNESRVESYREARAALNLMMNDLKSLYASTN
                     ANLFQTNAASPFLAVPGGGQLFFIAALPASAQDSGSRSDLCEVGYFLKYGQSGLNPDE
                     KNFSLYRYFRESNQTFTNLVNNSGFFTHSTANVELLARNIPNFQVRYYTVSTNGAVSP
                     WTAVSSNALPSFVELQITAFNNQTAKRFNSKEDWTNAASQTYRESSRVFTARIPLRPP
                     G"
     gene            2724..>2957
                     /locus_tag="VIS74_00125"
     CDS             2724..>2957
                     /locus_tag="VIS74_00125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5791669.1"
                     /translation="MPAQSAKRSLRQKASSLVTTLMVIVVLTIIVVAFMQSMSIERLT
                     SRSYANKYQAELLADAATEDAINRVFSSVFEAPH"
BASE COUNT          561 a          862 c          959 g          575 t
ORIGIN      
        1 gtgccggatt tccacgccgg gcagccggcg cgcggcctgg agaagtccgg cggcttcctg
       61 gaggagaatg ccaagccgcc ccatgccggt gtcaattttc aggtttaccg gcaacggggt
      121 gcggccggcc agcgccgcat aggccgcgat ttcttcataa cgcgagacgg tgggaataaa
      181 acccgcggcg gcgatggccg ggcgctcctc cggcagcgcg gggctgagaa tgagaatcgg
      241 ccggccggga accccctgcc gcaatacgcg ggcttcgttc aggttggcca ccgcgaaata
      301 ttccgcggag cgggccagcg cgcgggcggc ggtgattgcc ccatggccat aggcatcggc
      361 tttcaggacg gcgatgatgc cggcatccgg gcaatacttc cgcacgatcc gggcattcca
      421 ttcgatcgcg gcggcggaga tttccgtcca ggtccggggc ggagcagggg cgtcggtggc
      481 gaatttcaca gggcggcaag tgtggcggct ccggttttaa actgcaaccc ctgtctcggg
      541 cggcttgggc ggcgatgcgc tccgggaaaa agcaaagaga ttgtcacatc ggcgcggttg
      601 ccactgagag gacaggttgg catgaaagta gctgatcttg tttttcggaa tgaaaaaaga
      661 aagcgcatcc ggtggcgttt cctccaggaa ctcaccccgc gggttttcat tggtggagat
      721 gctcgcggtc atggcgatcg tgacggttct cctggccatg ctggcgccgg cggtgcgcag
      781 tttttcggat accgctggcc ggcgcggcgc cgtgaatgtg gtcatgaata ccctcgagca
      841 ggcgcgggtt tcggccctgg aaagcgggcg ggaagtgcag gtgctttttt accgggtcgc
      901 cttcccccac gaggatcggc tggtggtcat tcgtgagctg gaagacggct ccggatacga
      961 gcaattgacg aaatggctga agctgccgaa gggggttctc ctcttcaaac ccaccgccgg
     1021 gcattcggtg tttgaatccg ccgtgccagc cagtgttttg accgcattgc ccgcgaatct
     1081 ttccacctcc gctttgggga atgcgggcat ccgggtggtc aacgggatcg cggcgatcaa
     1141 attcaacgcc agcggccagg tggcgtatcc gacggacagc agccagttgc tgctccacct
     1201 cagcgaaggg gtgcgggatg aggacggcgt cgaagcgcgg atcacccaaa agcaggcggc
     1261 gagtctgcct tttgaaatta ttagtctctc caaatacacc ggccgtgccc agttggacat
     1321 cacggagctt gccgccaatt aaggttcgcg tcccggttgc ccccatgagc cgttcttttc
     1381 cagggcgcgg aggattttcc ctggtggaaa tcaccctggc gctgggcatc gtggcctttg
     1441 cgctggtggc catcatgggg cttttcccgg tggcgatgaa gaacgccctg gagagccaga
     1501 acgaaacccg ggcgaccttc atcgcgcaga gcattttttc cgacctggaa tcggggccgg
     1561 cttcgaccaa tacctttctg gcggcgggaa ccaactggag cagtccgggc gaccgggtga
     1621 tcgtgaacct gaaggcggcg aatacgtttt acgtgaagta ttccgacgaa ggcgcgccgg
     1681 tgggcactta ctcgaacgtc tccgcgttcg aggccgccgg gggagacgcc gcctacgggg
     1741 cgcgggtgat catcacgccc gatccggtgc ttgccaatct cagccgggcg gaggtgcagg
     1801 tggagtcgcc gttgagcgcg gcctcggcca gccgcgcgaa atttaacttc gtcacgcttc
     1861 tgccaaacaa cctcgtgaat acgaaccaat gagcgcgaag gggaaaaatc cggcggtggt
     1921 tcgcggcatc ggcattctgc cggtgcgggg agcgggttgg atcatcggca ggatgccgat
     1981 gccaccattc gggaggcggc gggctttcac cttgctggag ctgctggtgg ccgtgtcgct
     2041 gctggccatc ctggtggtca tgctcatgag catggtggac aatgcgacga agctctggcg
     2101 ggtgaacgaa agccgcgtgg agagttaccg cgaggcgcgg gcggcgctga acctcatgat
     2161 gaacgatctc aagtccctct acgcgagcac gaacgcgaat ttgttccaga cgaatgcggc
     2221 aagcccgttt ttggccgtcc cgggtggcgg gcagctcttt ttcatcgccg cgctgccggc
     2281 gagcgcccag gacagcggca gccgcagcga cctgtgcgag gtgggttatt ttttgaaata
     2341 cgggcaaagc gggctgaatc ccgacgaaaa gaatttcagc ctttaccgct attttcggga
     2401 gagcaaccag acctttacca atctggtgaa taattcggga ttcttcacgc attccacggc
     2461 caacgtcgaa ttgctcgcgc ggaacatccc gaatttccag gtccgttatt acacggtgag
     2521 cacgaacggc gcggtctcgc cctggacggc ggtttcgagc aacgcattgc ccagtttcgt
     2581 cgagcttcag atcacggctt tcaataacca gacggcgaag cggtttaact cgaaggagga
     2641 ttggacgaat gccgccagcc agacctatcg cgaaagctcg cgcgtgttca ccgcccgcat
     2701 tcccctgcgt ccgcccggat aacatgcccg cccaatccgc aaaacgctcg ttaaggcaaa
     2761 aggcctcgtc gctggtcacc acgctcatgg tcatcgtggt cctgaccatc atcgtcgtgg
     2821 ccttcatgca aagcatgtcc atcgaacgcc tgacgagccg gagttacgcg aacaaatacc
     2881 aggcggagct gctcgcggat gccgccaccg aagacgcgat caaccgcgtt ttcagttcgg
     2941 tattcgaggc acctcat
//
LOCUS       DATDAY010000006         4246 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_339243, whole genome shotgun sequence.
ACCESSION   DATDAY010000006 DATDAY010000000
VERSION     DATDAY010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 4246)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4246)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4246
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_339243"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            288..539
                     /locus_tag="VIS74_00130"
     CDS             288..539
                     /locus_tag="VIS74_00130"
                     /inference="COORDINATES: protein motif:HMM:NF015947.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HEY5791670.1"
                     /translation="MKALVTSKGQVTIPKALRDRFGIEPGAQVDFAPAEDGIRLRKVV
                     DRRKQAKVFGCLKAELEERSVAELLEALRGPVELPEKRQ"
     gene            536..946
                     /locus_tag="VIS74_00135"
     CDS             536..946
                     /locus_tag="VIS74_00135"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HEY5791671.1"
                     /translation="MSAVTIDTSVLLAIFKGETKGKLWIESLQSAAEAASLLVSSVAF
                     AEVRSFFPADRACRAALLDLDVRHSAINEEPANLAGKIFRDYRNEGGPRTTILPDFLV
                     AAHAATQADALAADDRGYMRAYFPNIRILAPATS"
     gene            1853..>4246
                     /locus_tag="VIS74_00140"
     CDS             1853..>4246
                     /locus_tag="VIS74_00140"
                     /inference="COORDINATES: protein motif:HMM:TIGR04286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MSEP-CTERM sorting domain-containing protein"
                     /protein_id="HEY5791672.1"
                     /translation="MAGAFIQGQGTAGQILFIGAYLALAALAAGGVLRVCVSIYVWTR
                     SRSPLGLAILTTVVALLGPLGGLLLNQWIRFPSDFQMPAVYALAILNGLLLVLPNFSH
                     PLLHRAVWLAQCAMFAFTAYFFAVFLPLLPLAPLSLLFLGFGSLMYVPSLLFLLHGYR
                     LLDGFRAEVRDGGKWLPALLGISALLAWPAGYTLQARGDRAALHQALDYLQYPDYSRN
                     ARFSGSLGALHSSLIHIRDFKQGFYLPYLSEYYNWLAFDNLTLSQNKLDALAKTFFGQ
                     PLPKLEAKQDFALFLRGNGRARNVSEALGGIQGERPSANAVIENITTATKLDRGMART
                     TAAITIHNPAAGATEFQSAISMPPGVMISGMWLTIGTERVPGRIFEERSALWVYQKIT
                     EVRPVARDPAILRYTGPAVAELRVYPVESGAPRQVEVEFLYPDGLDPDIRIGARELAI
                     APSETPSAPSLGVSGDGSFSVTLPEKAAAAFPKVSRKPYLHFLVDVSANSKFRDPELL
                     KEAIRQAAGAFPSAPSAREARLSFVNFETRDFGMDGRLPISELLNLPANDFRPPRDFF
                     RGGFLGFRAVKETLWRHHRALSASDGQALKSFPRIVILHGSSDPMPPDETGNLAEFAR
                     LLPDSAGFWSFKNGNLPMEFTPFDPRHQGEDDRPVRIFQVGGERFAASLDGPVSYTSI
                     RAAISSLEKIEAFDAASGRFVPAGPPLVQPASPDYNRAIGPWSLEFGRIFEPFAHRGD
                     ALGTLLKLCRETGILVPSVAYMVVENTAQWRILERTEKKALKGHEALALSEPAVTPEP
                     "
BASE COUNT          717 a         1327 c         1265 g          937 t
ORIGIN      
        1 cagctggagc ggcgaccggg gtctttatgt tccaaatttt catcgcgaaa ccgagccttc
       61 ctttgacaga aagccgcccg ccggagcaag cgcaaagggt gccgggggga tgagcggccg
      121 aaagttcccg atcgaccgcg ggcaaagcat cgcccctgcc agcctccctc tcttgagagc
      181 tggcgcgttc atttccagcc gatccagcgg cagaaatccg tcacgagagg cttcccgatt
      241 cgaaatgtaa tacctgcaat tcttacaaaa tattctaccc taaacgcatg aaagctctcg
      301 tcacttcgaa agggcaagtc accatcccta aagcacttcg tgatcgtttc ggcatcgagc
      361 ctggcgcgca ggtggatttt gcgcccgcgg aagatggcat ccggttgagg aaagtggtgg
      421 atcgccgcaa gcaagcgaag gtcttcggct gtttgaaggc agagcttgag gaacgcagcg
      481 tggccgagtt gctggaagca ctgcgtggtc cggtggaact gcccgagaaa cgccagtgag
      541 cgccgtcacg atcgatacct ccgtcctgct ggccatcttc aaaggagaaa cgaagggcaa
      601 actttggatc gagagtcttc agtccgccgc ggaggcagcc tccctcctcg tgtcctccgt
      661 ggccttcgcg gaagtccgat cttttttccc ggctgaccgg gcctgtcgag cggcgcttct
      721 cgatctcgat gtccggcaca gcgccatcaa cgaagagccg gcaaatctcg ccgggaagat
      781 cttccgcgat taccggaatg aaggagggcc gcgaacgacc attctccccg acttcctggt
      841 ggccgctcat gccgcgaccc aagcggacgc actggccgct gatgatcgcg gttacatgcg
      901 cgcttatttc cccaacatcc gcatccttgc tccggcaacg tcttgattca tcgaaagcgc
      961 gtttggcgca tgcccccccc acaacagaac ggctctctcc agcgcgcgca cgatcccact
     1021 tctgaaaggg cggcctcgcc gtcatttcgc ctgaccccac tattgagaca tgccgaaatc
     1081 cattacgtaa cgtcacccgc aggcttcctt cagggcgccg gaatcgaggc tcgtgcggag
     1141 tttttcaagt tgggcgatcg gcgctttgtc tccgccgtgg cgcagtattg acttcccccg
     1201 gcgcctcttt gcgggcattc tccgccggtg aagactccgc aggatacttc cgagattcct
     1261 cccgcgcccg ggcttgaatt ttcggcggcg gaaaggcggg ttgcctggcc gccgaaacag
     1321 cgattgaccc cgctcttgct ccccaaggcc gtgttgagcc catggatgat tgtgctgatc
     1381 ggggtggcgc cgcaaagtgc gctgctggcg ctgaacctcc aggcgtgggg cctggcggcg
     1441 ggcgaggtgt cgcctcagca actttccgcc gcccatctga tcctcgcctg ggagttgctc
     1501 gcgctcggca gcggcatcct gctggcgggg tggcttttca tgcgccggga aacgatgcgc
     1561 cgctggcagg gcgtgattcc gttgatctcc gccgcgcttt atctcgcggt ggtttttttg
     1621 gttacggacc aggccattcc cgcggcgctg gcggactgga tgattccgcg cgaccaatgg
     1681 atgctcaaac aattcgcgct ggtcatgccc gccgccctgt tcggggcgtt gagaatcttg
     1741 tgccctgacc gggacgaagc gagccagcgt gcgaagggga ttgctctcgc gattggcgtg
     1801 atgatcggcc tgctcggcgc ttgcatcggt ttcctttttc ctcgtggggc ttgtggcggg
     1861 cgcattcatc cagggccagg gaaccgccgg ccaaatcctt ttcatcggcg cctatctggc
     1921 actggcggcc ctcgcggcgg gcggcgtgct gcgggtgtgc gtcagcattt atgtctggac
     1981 gcgctcgcgc agtcccctgg gactggccat tctcacgacc gtggtcgccc tgctgggacc
     2041 gctgggcggg ctcttgttaa atcaatggat ccggttcccg tcggatttcc agatgcccgc
     2101 cgtttacgcg ctggcgatct tgaacggact tctgcttgtt ctgccaaatt tttcccatcc
     2161 gcttctccac cgggcggtgt ggctggccca gtgcgcgatg ttcgcgttca ccgcgtattt
     2221 tttcgccgtt ttccttcctt tgcttcccct ggctcccttg tcccttcttt ttttggggtt
     2281 tggcagtttg atgtatgtgc cctcgctgtt gttccttttg cacggctatc gacttctcga
     2341 cggattccgg gccgaagttc gggacggcgg caaatggctt cccgcgctgc tgggaatttc
     2401 cgccctgctt gcctggccgg ccggctatac gctgcaagcc cgcggggacc gcgccgccct
     2461 gcatcaggct ctcgattatt tgcaatatcc cgactattcg cggaacgcgc gtttttccgg
     2521 cagcctgggc gccctgcatt cctcgctgat ccacattcgc gatttcaaac aggggttcta
     2581 cctgccctac ctgtcggagt attacaactg gctggcgttt gacaatctga ccctgtctca
     2641 aaacaagctg gacgcgctgg cgaagacgtt ctttggccag ccgcttccga agctggaagc
     2701 gaagcaggac ttcgcgcttt tcctgcgggg aaacggccgg gcccgcaatg tttccgaggc
     2761 gcttggcggc attcagggtg agcgcccgag cgccaatgcc gtcatcgaga atatcacgac
     2821 ggccacgaag ctcgaccggg gaatggcgcg aaccacggcg gcgattacga tccacaaccc
     2881 ggccgcgggc gccacggagt tccagtcagc catttccatg ccgccgggag tcatgatctc
     2941 cggcatgtgg ctgacgattg ggacggagcg cgtccccgga cgcattttcg aagaacggag
     3001 cgccttgtgg gtgtatcaaa aaatcaccga ggtcaggccg gtggcccgcg atcccgccat
     3061 actgagatac acggggcccg cggttgccga gctaagggtc tatccggtcg aatcaggggc
     3121 gccgcggcag gtggaagtgg agttccttta tcccgacggc ttggatcccg atatccggat
     3181 cggcgcgcgg gaacttgcca ttgcgccctc cgaaacgcct tccgccccga gcctgggggt
     3241 atccggcgat gggtcttttt ccgtgacgct tcccgagaaa gcggctgccg ctttcccgaa
     3301 agtttcccgg aaaccttatt tgcatttcct cgtcgatgtc tccgcgaatt cgaaattcag
     3361 ggatccggaa ttgctgaaag aagcgatccg gcaagccgcc ggcgcctttc cttccgcgcc
     3421 ttccgcgcgc gaagcccgat tgagtttcgt gaattttgaa acgcgggatt tcggaatgga
     3481 cgggcgattg cccataagcg aactgctcaa tcttccggcg aacgatttcc gtcctccgcg
     3541 cgatttcttc cgcggcggct tcctgggttt tcgggcggtc aaggagactt tgtggcggca
     3601 tcatcgggcg ctttcggctt ccgatggcca ggcgttgaaa tcgtttcccc gcatcgtgat
     3661 tttgcacggt tcttccgacc cgatgccgcc cgatgaaacc ggcaatctgg cggaattcgc
     3721 ccgcctgctt cccgacagcg cgggtttctg gtctttcaaa aacgggaact tgcccatgga
     3781 gttcactccg tttgatcccc gccaccaagg tgaagacgac cggcccgtcc ggatttttca
     3841 ggttggcggc gagcgatttg cggcatcgct tgacggcccc gtgtcttaca ccagcattcg
     3901 cgccgcgatc tcatctcttg aaaaaattga ggcgttcgac gccgcatcgg gccgcttcgt
     3961 cccggccggg ccgcctctcg tgcagccggc gtccccagat tacaaccgcg caatcggccc
     4021 ttggagcctg gaattcgggc gcatttttga gccgtttgcc catcggggag acgcgctcgg
     4081 gacgttgctc aaactctgcc gcgagaccgg aattctcgtg ccttccgttg cctacatggt
     4141 cgtcgaaaac accgcccaat ggaggatatt ggagcgaacc gagaaaaaag ccctgaaggg
     4201 gcatgaagcc cttgcgttgt ccgaacctgc cgtgactcct gagcct
//
LOCUS       DATDAY010000007         2025 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_332728, whole genome shotgun sequence.
ACCESSION   DATDAY010000007 DATDAY010000000
VERSION     DATDAY010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2025
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_332728"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..382
                     /locus_tag="VIS74_00145"
     CDS             <1..382
                     /locus_tag="VIS74_00145"
                     /inference="COORDINATES: protein motif:HMM:NF012696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HEY5791673.1"
                     /translation="AIHTLKIKHIIVCGHYGCGGVRAALDNSSHGLIDNWLTKIRHTY
                     ARHQGEIDALPTREARVDRLCELNVVDQVRSVSQTTIVQEAWARGQTVNIYGWCYWLK
                     NGLVNDLGVKVSSLDEVPPAYRVV"
     gene            387..>2025
                     /gene="recQ"
                     /locus_tag="VIS74_00150"
     CDS             387..>2025
                     /gene="recQ"
                     /locus_tag="VIS74_00150"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HEY5791674.1"
                     /translation="MPELLQTLKSAFGYSEFRPLQREIMETSLRGRDVFALLPTGGGK
                     SLCFQLPALLRPGLTLVVSPLIALMKDQVDQLEATGVPATYLNSSLTAAETKRRLRGL
                     HEGEYRLLYAAPERLMLDHWQENLRAWNVSALAIDEAHCISEWGHDFRPEYRQISQLR
                     DWLPNIPFMALTATATERVRADVIRHLRLREPEIFVASFNRPNLTYRVIAKDQPGRQI
                     AEFVRARREESGIIYCASRSAAERVAESLHERGYPVRPYHAGLSAEERSENQERFLRD
                     EVRIICATIAFGMGINKPNVRWVIHHDLPKNIEGYYQETGRAGRDGLPGECLLLFSPG
                     DIAKQTHFLEEISDPHERQVARAQLRTMVHYAECAQCRRRELLEYFGERFEPENCAAC
                     DNCLEPRETYDGTVLAQKFLSCVYRIRQQSAFGVGINHVGEVLTGADTEKIRRWGHHT
                     LTTYGVGRELSRPAWATVARELLRLGLLEQSSGEYPTLELTGEGLDALKSRRAIALTK
                     AMWKPQTAPEKTRRSSGEIPCDEALFARLRNLRKEIAD"
BASE COUNT          372 a          680 c          648 g          325 t
ORIGIN      
        1 cgccattcat acgctgaaga tcaagcacat catcgtttgc ggccactacg gctgcggcgg
       61 cgtgcgcgcg gcgctggaca attcctcgca cggcctcatc gacaactggc tgaccaagat
      121 ccgccatacc tacgcgcggc atcagggaga gatcgacgcc ctgccgacgc gcgaggcccg
      181 ggtggaccgc ctctgcgaac tcaacgtggt ggaccaggtc cggagcgtga gccagacgac
      241 gatcgtgcag gaagcctggg cgcggggcca gacggtgaat atctacggct ggtgctactg
      301 gctgaaaaac ggcctcgtga acgatctcgg cgtaaaggtt tccagcctgg acgaagttcc
      361 gcctgcctat cgcgtggtgt gagcttgtgc ctgagctgct gcaaaccctc aagagcgcct
      421 tcggttattc cgagttccgc ccgctccagc gcgagatcat ggagacctcg ctgcggggcc
      481 gggatgtgtt tgccctcctg ccgaccggcg gcggcaagtc gctctgtttt cagctgcccg
      541 ccttgctccg gccggggctg acgctcgtcg tctctccgtt gatcgccctg atgaaggatc
      601 aggtggatca gctcgaggcc accggcgtgc cggccacata tctgaactct tccctcaccg
      661 ccgcggaaac caaacgccgc ctgcgcgggc tgcacgaagg tgaataccgc ctgctctacg
      721 ccgcgccgga gcggctcatg ctcgaccatt ggcaggagaa tctgcgcgcg tggaacgtgt
      781 cggccctcgc catcgacgaa gcccattgca tttcggaatg gggccatgat ttccggccgg
      841 aataccgcca gatttcccag ctccgcgact ggcttcccaa cattcccttc atggccctga
      901 ccgccacggc caccgagcgg gtgcgggcgg acgtcatccg gcacctgcgg ctgcgcgagc
      961 cggaaatctt cgtggccagc ttcaaccggc cgaatctgac ttatcgcgtc atcgcgaagg
     1021 accagccggg gcggcagatc gcggaattcg tccgcgcccg ccgggaggag agcggcatca
     1081 tttactgcgc gagccgttcg gcggcggaga gggtggcgga gtcgctgcac gaacgcggtt
     1141 accccgtccg gccctaccac gccggcctca gcgcggagga gcgctccgaa aaccaggaac
     1201 ggttcctgcg ggacgaggtc cggatcattt gcgcgaccat cgccttcggc atgggcatca
     1261 acaaacccaa cgtccgctgg gtgatccacc acgatctgcc gaagaacatc gaaggttact
     1321 accaggaaac cggccgcgcg ggccgcgacg ggctgcccgg ggaatgcctg ctgctcttca
     1381 gccccggcga catcgccaaa caaacccatt tcctggagga aatttccgat ccgcacgaac
     1441 gccaggtcgc ccgcgcgcag ctgcggacca tggtgcatta cgccgaatgc gcccagtgcc
     1501 gccggcgcga gctgctggag tatttcggcg agcgcttcga accggaaaac tgcgccgcgt
     1561 gcgacaactg cctggagccg cgcgagacct acgacggcac cgtgctggcg cagaaatttc
     1621 tctcgtgcgt ataccgcatc cgccagcaaa gcgccttcgg cgtcggcatc aaccacgtcg
     1681 gcgaagtgct gaccggggcc gataccgaga agatccgccg ctgggggcac cacaccctga
     1741 ccacctacgg cgtcggccgg gagttgagcc ggccggcctg ggcaacggtc gcccgcgagc
     1801 tgctgcggct cgggctgctg gagcagtcgt ccggcgaata tcccacgctc gaactgaccg
     1861 gggagggttt ggacgcgttg aaatcccgcc gcgccattgc gctgaccaag gcgatgtgga
     1921 aaccccagac cgcgccggaa aaaacgcggc ggagcagcgg ggaaatcccc tgcgacgaag
     1981 cgctcttcgc gcggctgcgg aatctgcgca aggaaatcgc cgacg
//
LOCUS       DATDAY010000008         4542 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_2619405, whole genome shotgun sequence.
ACCESSION   DATDAY010000008 DATDAY010000000
VERSION     DATDAY010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 4542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4542
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2619405"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..341)
                     /locus_tag="VIS74_00155"
     CDS             complement(<1..341)
                     /locus_tag="VIS74_00155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5791675.1"
                     /translation="MPPGSIATPLAPPPFRQCLANLFRIAAPALCAALLGSCSAPRLA
                     GGGRMPPIRGALLPPNVHGRLVARTMTPRYLTIHATENLNSGADNHAGYLSNKGKRSS
                     NRRFGRSGWVT"
     gene            complement(432..1265)
                     /locus_tag="VIS74_00160"
     CDS             complement(432..1265)
                     /locus_tag="VIS74_00160"
                     /inference="COORDINATES: protein motif:HMM:TIGR00368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HEY5791676.1"
                     /translation="MATIIPPMTMEEAIETTKIHSVCGLLDDRNSFVRQRPYRAPHHT
                     ISDIGLLGGSTNPTPGEVSVAHNGVLFLDELPEFKRSTLEVMRQPLEDGKVTISRAAG
                     SLTFPARFMLVAAMNPCPCGHFGNPKRECRCSPAQVERYRQKISGPLLDRIDIHVEAP
                     AVEYKELSATESGESSAAIRERVVQVRAVQRGRFAASRTRANAQMTPAQQRKFCALDA
                     ECAGLIERAMTDLNFSARAYDRILKVARTIADLAGAEAIRTPHLLEAIQYRTLDRTLW
                     S"
     gene            1225..3177
                     /locus_tag="VIS74_00165"
     CDS             1225..3177
                     /locus_tag="VIS74_00165"
                     /inference="COORDINATES: protein motif:HMM:NF012464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEY5791677.1"
                     /translation="MASSIVIGGMMVATRLASMLLPEPGGPMSSNIFYNNPQSAYDEE
                     MKPLAFSYLRFSTPEQALGHSERRQIEAAEKWAKKKSLKLDKSYADRGRSGFKGTNRK
                     KGALGEFLKRITAGNIPRGSYLVVEDLDRLSREHPMDSLTLVRNIISAGVTIAVLKGN
                     EEYSEEVLRADRSGMKMMGLIFELGRASGESARKADLGSKNWGQKRKQAAEHGRVMTE
                     IAPAWIGTTGEKENRKFILIEDRAEIVRQIFKWHLRGTGNRTIAQKLNDGKIPNWGRG
                     KRAATHWHPSYIQKILHNPGVIGELHPHKLEYVPDPTNASEQIQISKPTGEIFKDYYP
                     AVVDLDTYQRVQTTNRHPSRGNHGGRIQKFVSNLFPSLTFGKLEDPTGSIPHPEEPAP
                     PPTVQVPARYKSKGSHGHGQYLVAEVDAVNKKRKRKDEIAPARWPYPAVEFAILKTLQ
                     EIDWKVVAGHGNSPAQTAFASRVAAIERTADELRAKCENLADVIANTPLPSLIRKLEE
                     LEEKRKEAKANATEARKELADSEISKRGLAVPLSIKDAAHDPTARDVRLALRAEIARR
                     IKSIEISRKVLVPKKDHRDRITHAHSFQISIYFVNGASRFIRVTLNKGKEPTIQSVNF
                     QENGKGKSLKLASIESKEPRGKPRGI"
     gene            complement(4097..4504)
                     /locus_tag="VIS74_00170"
     CDS             complement(4097..4504)
                     /locus_tag="VIS74_00170"
                     /inference="COORDINATES: protein motif:HMM:NF012730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion transporter"
                     /protein_id="HEY5791678.1"
                     /translation="MATRGFFASLGVLAILALSLLAPLSPEIKRILEIADTTICFVFL
                     ADFGLLLYLHKNRKKYFFTWGWIDLISSIPLFDPLRWGRLARMVRLLRLFRGLRGSTG
                     LLRRLLLSKQETTLAAIFLLIAAVMVPTGTVGK"
BASE COUNT         1145 a         1347 c         1244 g          806 t
ORIGIN      
        1 cacgtcaccc agccggagcg tccgaaacgc cggttgctgc tgcgtttgcc tttattcgag
       61 aggtaacccg cgtggttatc ggcgccggag ttaagattct cggtggcgtg gatggtgagg
      121 tagcgcggag tcatggtccg cgcgacgagg cggccatgga cattcggcgg caggagggcg
      181 ccgcggatgg gcggcatgcg tccgcccccg gccagccgcg gcgcggagca ggaccccagg
      241 agagcggcgc atagcgccgg ggcggcgatg cgaaaaaggt tcgccaggca ttgcctgaac
      301 ggaggaggcg caaggggggt ggcgatggaa ccaggcggca tttttaaact cccttctcca
      361 ttgcgaaagt ggtttgggtc aggaaaaggc catcccacat aagtgcctca tcggtttgat
      421 gcttcaatca atcagctcca caaggtgcga tccagcgtcc gatattggat ggcttcgagc
      481 agatgcggcg ttcggattgc ctccgccccg gccaggtcgg cgatggttcg cgcgaccttg
      541 aggatgcggt catacgcccg cgcgctgaag ttcaggtcgg tcatggcgcg ctcgatcaat
      601 cccgcgcact cggcgtccag cgcgcagaac tttcgctgct gggccggggt catctgcgcg
      661 ttcgcgcggg tgcgggaggc ggcgaatcgc ccgcgctgca ccgctcgcac ctgaaccacc
      721 cgctcgcgga tcgccgccga ggattcgccg gattccgtgg cggaaagctc cttgtattcc
      781 accgccgggg cttcgacgtg gatgtcgatg cggtccagca gcggcccgga aatcttctgc
      841 cggtaacgct cgacctgcgc cggcgagcag cggcattccc gcttcgggtt tccgaaatgg
      901 ccgcaggggc acggattcat cgccgccacc agcatgaagc gcgcgggaaa ggtcagactg
      961 cccgccgcgc ggctgatggt cacctttccg tcctccagcg gctggcgcat gacctccagc
     1021 gtggagcgct tgaactcggg caattcgtca aggaacagca cgccattgtg cgcgacgctc
     1081 acctcgcccg gcgtgggatt ggtcgagccg ccgagcaggc cgatgtcgga gatcgtgtga
     1141 tgcggcgcgc gatacggccg ctggcgaacg aaggaattcc ggtcgtcgag caggccgcag
     1201 acgctgtgga ttttcgtcgt ctcgatggct tcctccatcg tcatcggcgg aatgatggtc
     1261 gccacccgtt tggccagcat gcttttgccc gaaccgggcg ggccgatgag cagcaacata
     1321 ttttacaata acccacaatc ggcatatgat gaagaaatga aacctctcgc cttttcttat
     1381 ctccggtttt ctacaccgga acaagcctta ggccattccg agcggcggca aatcgaagcc
     1441 gccgaaaaat gggccaagaa gaaaagcctc aagctggata aatcctacgc cgaccgtggc
     1501 cgctcaggct tcaaaggcac aaatcgcaaa aaaggcgctc tcggcgaatt cctcaaacgc
     1561 atcaccgccg ggaacatacc tcgcggctct tacctcgtcg ttgaagacct agacaggctt
     1621 tcgagagagc atcctatgga ttcgcttacg ttagtgcgca acatcatttc cgctggagtc
     1681 accatcgcag tgctcaaggg taacgaagaa tactccgagg aagtcctacg cgcagacaga
     1741 agcggcatga aaatgatggg cctcatcttc gagctagggc gggcttccgg ggaatcagcc
     1801 cgaaaagcgg atcttggttc caaaaactgg ggccaaaagc gcaaacaggc agcggaacac
     1861 ggcagggtga tgaccgaaat cgccccggca tggattggca cgacgggaga aaaagaaaac
     1921 cgaaaattca tcctgatcga agacagagca gaaatcgtcc gtcagatatt caaatggcac
     1981 ctgcggggaa ccggcaaccg gacaatcgcc caaaagctga acgacggaaa aatcccgaat
     2041 tggggacgag gaaaacgtgc cgccacgcac tggcacccgt catacatcca aaaaatcctg
     2101 cacaaccccg gcgtaatagg ggagctccat ccgcataaac tcgaatacgt gccagacccc
     2161 accaacgcat ctgagcaaat ccaaatcagc aagccgactg gcgaaatttt caaagactac
     2221 taccccgccg tagtggattt agacacgtac cagcgcgtac agaccacaaa ccggcatccc
     2281 tctcgcggaa atcacggcgg gcgaatccaa aaattcgttt ccaatctctt ccccagcctc
     2341 actttcggaa aattggaaga tccgacagga agcattccac acccggaaga accagcccca
     2401 cccccgacag ttcaagtccc tgcgcgctac aagtcaaaag gaagtcacgg gcacgggcaa
     2461 tatcttgtag ccgaagttga cgctgtgaac aaaaaacgga aacggaagga cgaaatagcg
     2521 cctgcccgct ggccataccc cgccgtcgaa ttcgccattc tgaaaaccct gcaggaaatc
     2581 gactggaagg ttgtggctgg tcatggcaac tccccggcgc aaacggcttt tgcgtcccgc
     2641 gtggccgcca tagaaagaac cgccgacgaa ttgagggcaa aatgcgaaaa tctggcagat
     2701 gtcatcgcaa acacacccct ccccagtcta attcggaaac ttgaggaact ggaagaaaag
     2761 cggaaggagg caaaggccaa cgccaccgaa gccagaaaag aattggcgga ctcggaaatt
     2821 tcaaagcgcg ggctcgccgt gccactttcc ataaaagacg ccgcgcacga tccgacggcc
     2881 cgagacgtac ggctagctct ccgcgccgag attgcccgac gcatcaaatc aatcgaaatc
     2941 tctcgcaaag tactagtccc gaagaaggac caccgagaca ggatcactca tgctcactcg
     3001 ttccaaatct ccatttactt cgtgaatgga gcctcacgtt tcatccgtgt gaccctgaat
     3061 aaaggcaagg aaccaactat tcaaagcgtc aatttccaag aaaacggaaa agggaagtcc
     3121 ttgaaactgg cgagcattga aagtaaagaa ccccggggca agccccgagg catttaagaa
     3181 tatgaattca gagagccacg aaacaaagcc gaagcaagct tcggggtatc agacccaaag
     3241 ggaatgaaga cagtcaatga catgcaggcc aaaaaaacag ggctcatttg atctgaatac
     3301 agcaggttac tgacgagaga gtccaaattg agaagtctca tttcctgcac accccatccc
     3361 caaaacctac aggggcgaaa atttcgccaa tcgacgcgtc agagcccgcc aggttttcga
     3421 gccctacggt cagagactca aaggaagcaa tcccctgaca cccctgattg cttcctttga
     3481 gactcttcga ccgatcccgg gggcggccaa gcctccttcg tcggcttcgc cgccgcgcct
     3541 gcggcgcacg cgccctccgg gcgtagatca agtccctgcg gtagtggaaa aaccctccag
     3601 cggccatcgc ctacatccct gatgaatcgc caactcaaga ccagatcagg aacactgaat
     3661 atttgaatct gacaggacac atttcaattc agaataccgc caaaaaaaca agcggatgag
     3721 cagataagat tattatgctc cgcagactcg cgcaaatttc aaaatattta cccgtaactc
     3781 caggcggaag gtgattttcc gcaaagccgg aaaacgcttc gcgccttccc gctcagtcaa
     3841 acggtaaaaa tcgaatcgac gcggcagagc ccgccaagtt ttcgagccct gcggtcagag
     3901 actcaaggaa agcaatcccc tgaaaccccg attgctttcc ttgagactct tcgaccgttc
     3961 ccggggcggc caagcctcct ccgctacgga atcgaccctt cagctacgcc ttgcgtcccc
     4021 tacgcttttg gttcaattcc tccgctccgt cggcttcgcc gccgcgcctt cggcgcacgc
     4081 gccctccgtg cgtaggtcat ttccccacgg ttcccgtggg taccataacg gcggcgatca
     4141 gaagaaaaat tgcagccagt gtggtttcct gtttcgagag aagcagtcgg cgaagaagcc
     4201 cagtgcttcc gcgcagcccc cggaagagcc tgagtagcct caccattcgc gcgagcctgc
     4261 cccatcgcaa ggggtcgaaa agtgggatgg atgaaatcag gtcaatccac ccccatgtga
     4321 agaaatattt cttccgattt ttgtggaggt agagaagaag gccaaaatcg gcgagaaaca
     4381 caaaacagat ggtggtatcg gcaatctcca gaatccgttt gatttccggc gaaagcggcg
     4441 ccagcaaaga caacgccaaa atcgccaaaa ctccgagtga ggcaaaaaaa ccacgagttg
     4501 ccaagcaact gggacaggtc ttctcaggtg ctcgtcatcc at
//
LOCUS       DATDAY010000009         5174 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_2618523, whole genome shotgun sequence.
ACCESSION   DATDAY010000009 DATDAY010000000
VERSION     DATDAY010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 5174)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5174)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5174
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2618523"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            191..1987
                     /locus_tag="VIS74_00175"
     CDS             191..1987
                     /locus_tag="VIS74_00175"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HEY5791679.1"
                     /translation="MRVLPHSTLSAAVASLCLNGLALAQQPAPTPAGGNLIANGGFEK
                     SFKRDNPWPGISAAGFLEGQRGTLPVLTQSGVIAETSMPVSVAVADMNADAKPDIVAA
                     DVLGYFRVYFNQGTAQEPKFADADLIPVFLSRTPHGSDQFRYSGPRISVSDFARTGKN
                     DILFGNYIGQILWLKNQGSISSPGFQQPKTADSILVPTTQDSQRRWGNVFAPAAWDWN
                     RDGKFDLLLGEGSYSANNIHLLLNQGSGALPKFDETNRHFLAYGDGREQLTPAVEDYN
                     GDGRPDVLVADRAGKIGLYLNDQPNWKPGDEIKFTSYVPIQGQAGKDLTFGGICTVTT
                     GDLNGDGLFDLVVGKTNGRITVAYNRGTQAEPKFDAPIELKGETKASPFQPPSGWEVD
                     FGLNRGNFLGYGNAESNPQASAPEGKQALRLGYLPNSNAVIKTPFAYLPALDAKWTSQ
                     IGENGAWAGMIGNSPANYFFVTPSGKLNLTPGKSYVMSFKVKGTGATDTKVFIAYGIE
                     VQLGESRVVQGERNSAKVERNLVGENKLETLPFSSSPGWTTVTKEFKVQFTNKELNDP
                     AQKAGVGILFVAALAPGKGELYIDDVQLTEKK"
     gene            1984..2961
                     /locus_tag="VIS74_00180"
     CDS             1984..2961
                     /locus_tag="VIS74_00180"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HEY5791680.1"
                     /translation="MSSPRIRQAFILGAGLGTRLRPLTDCRPKPLVPILNKPLITFAF
                     DHLKQAGITRLAVNTHHLPESYERILGAARQELDYFGSRVHFCHEPVLLETGGGIANV
                     RPLLDDTAPFLVYNGDVLCDAPLGRLIERHLAEGNAATLLLRSSGGPLQVQCDPQTGR
                     IEDVRQMLGGSTAPGFLFSGIYVLDPRIFRWIPAGEILSIIPVFLKMIQSGEKIGGVV
                     EDGGRWFDLGNPESYVRTCRTMADPENAPAYPLHWPMRAIHLEADIAADARFEGICSV
                     GARSRIGPDTRLTDTFVWDRATLADHSRFRGCIVRDGRQAGGNQEDCIP"
     gene            2958..3932
                     /locus_tag="VIS74_00185"
     CDS             2958..3932
                     /locus_tag="VIS74_00185"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEY5791681.1"
                     /translation="MINGSIVSATRRQFPHFTNQALEVIPLEKGGSDRKFHRIRTGEA
                     SMIFIQYSDSREENSHYVEIARFLRSQGLRVPEVYHHDAKSKIIWMQDLGEVDLWHFR
                     QQPWPVRRPHYLSTLEQAARLHTTATAARPGAPIQLQPAFDEALYRWEQAYFFENCLQ
                     LALGFAEAEARRQAENPAFARIAARLAGRAPTLVHRDFQSQNIMICQDQAWLIDFQGM
                     RPGLPQYDLASLLYDPYVTLTGEERRDLLAAYQAFAGVGAGPDFEEIFDLCALQRLMQ
                     ALGAYGFLGLKRGKPHFLNHIPAAKKSLAEVAARIAGLGDFVALLRQS"
     gene            3929..>5174
                     /locus_tag="VIS74_00190"
     CDS             3929..>5174
                     /locus_tag="VIS74_00190"
                     /inference="COORDINATES: protein motif:HMM:NF023324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltotransferase domain-containing protein"
                     /protein_id="HEY5791682.1"
                     /translation="MNPKPQTVVIERPSPQLDGGRYPIKRIAGQDLRIEADIFKDGHD
                     VVRALAKWRRVGETKWHATEMQPLINDRWEGVLPLAEMGLYEYTIEAWGDSFESWRQE
                     FIKKSDAGITELTSEILTGAALAEAAAVRAKGTADAKPLKEHGKKLKAADAAQVRQWL
                     DAPDFLALMRHWPDRSFSTEFKPYLQARAERKRAAFGAWYEFFPRSAEGKADAGSTFR
                     DCLPRVEDAQAMGFDVIYFPPIHPIGVKNRKGRNNSLTPEPGEPGVPYAIGNREQGVN
                     GGGHCDVAPELGTLEDFDWLVGEIRKRGMEVALDFAIQCSPDHPWLRDHPEWFFHRPD
                     GTIKYAENPPKKYEDIYPINFHNANWQGLWNELRDTFRFWCRHGARIFRVDNPHTKPV
                     AFWEWVIAEVQREFPDAIFLSEA"
BASE COUNT         1014 a         1711 c         1524 g          925 t
ORIGIN      
        1 ctgctcggca tgctgacgag ctgcgccagc cgggtggcgg tggtgaacat tgacaacggc
       61 ttcggcgccg cctgcgcggc ggatgccatc ctgcggctcg ccgccgcgga ggctaaaaaa
      121 taaatggaaa caggttgcgc ggtggattta cttttcaaaa ccccacaacg catctaggtt
      181 tcgtcgttct atgcgagttc tccctcactc gaccctttcc gccgcggttg cgtctctttg
      241 cctgaacggc ttagccctgg cgcagcaacc cgcccctacc cccgccggcg gcaacctgat
      301 cgccaacggc ggttttgaaa aaagcttcaa acgggataat ccctggccgg ggatcagcgc
      361 ggccggcttc ctggaagggc agcgcggaac ccttcccgtg ctgacgcagt ccggcgtcat
      421 cgcggaaacc tccatgccgg tttccgtggc cgtggcggac atgaacgcgg acgccaaacc
      481 ggacatcgtc gccgccgacg tgctgggtta tttccgcgtt tattttaatc agggaaccgc
      541 gcaggaaccc aaattcgccg acgccgacct catccccgtc ttcctgagcc gcacgccgca
      601 tggctcggat caatttcgtt acagcggccc gcgcatttcc gtctcggatt tcgcacggac
      661 cggcaaaaac gacatccttt tcgggaacta catcggccag attctctggc tgaagaacca
      721 gggttccatt tcttcgccgg gttttcagca gccgaagacc gccgacagca ttctcgtacc
      781 gaccacccag gactcccagc gccgctgggg aaacgtcttt gccccggccg cgtgggattg
      841 gaaccgggat gggaaattcg accttctgct cggcgaaggc tcctactccg cgaacaacat
      901 ccacctcctg ctcaaccaag ggtcgggcgc gctcccgaaa tttgacgaaa ccaaccgcca
      961 tttcctggcc tacggcgacg ggcgcgagca attgaccccg gcggtcgagg actacaatgg
     1021 cgacgggcgg ccggatgtat tggtcgccga ccgcgcgggg aagatcgggc tttacctcaa
     1081 tgaccagccg aactggaaac cgggcgacga gatcaaattc acctcctacg tgccgatcca
     1141 gggccaggcg gggaaggatc tcaccttcgg cggcatttgc accgtcacca ccggcgacct
     1201 gaatggggac ggcctctttg atctcgtggt cggcaagacc aacgggcgca tcaccgtcgc
     1261 ctacaaccgc ggcacccagg cggagcccaa attcgacgcg cccatcgaac tgaaggggga
     1321 aaccaaggcc agccccttcc agcctccgtc gggatgggag gtggacttcg gcctcaaccg
     1381 gggcaatttc ctgggctatg gcaacgcgga gagcaatccc caggcatccg cgccggaagg
     1441 caagcaggcg ttgaggttgg ggtatcttcc caactcaaac gcggtcatca aaacgccgtt
     1501 tgcctatctc cccgcgcttg acgccaaatg gacgtcccag attggcgaga atggcgcctg
     1561 ggcgggcatg atcggcaata gtcccgccaa ctattttttc gttactccga gtggaaagct
     1621 gaacttaacc cccggaaaaa gctacgtcat gagcttcaag gtcaagggaa ccggcgccac
     1681 cgacaccaag gtcttcatcg cttacggaat cgaggtccaa ctcggagaaa gccgggtcgt
     1741 tcagggcgag cgaaacagcg ccaaggtgga acgaaacctc gtcggcgaaa acaaattgga
     1801 aaccctgccc ttcagttcga gtccggggtg gaccaccgtc accaaggaat tcaaagtgca
     1861 attcaccaac aaggagctga atgatcccgc ccaaaaggcg ggcgtcggca tcctgttcgt
     1921 cgcggcgctc gctccgggca agggcgaact ctacattgac gacgtgcagc tcacggaaaa
     1981 gaagtgagtt ctccgcgcat ccggcaggca ttcattctgg gcgcggggct gggcacccgc
     2041 ttgcggcccc tcaccgactg ccggcccaag ccgctggtcc ccattcttaa caagccgctg
     2101 atcaccttcg ccttcgacca tttgaaacaa gccggcatca cccggctggc ggtcaatacc
     2161 catcacctcc ccgaatccta tgaacggatc ctcggagccg cgcggcagga attggattat
     2221 tttggcagcc gggtccattt ttgccacgaa cccgtgctgc tcgaaaccgg cggcggcatt
     2281 gccaatgtcc gccccctgct cgacgacacc gcgccctttc tcgtgtataa tggcgacgtc
     2341 ctttgcgatg cgccgctggg ccggttgatc gagcgccacc tggccgaggg caatgccgcc
     2401 accctgctgc tgcgttccag cggcgggccg ctccaggtgc aatgcgaccc gcagaccggc
     2461 cggatcgagg atgtccgcca gatgctgggc ggcagcacgg cgcccggttt tctgttttcc
     2521 ggcatttatg tgctggatcc gcgcattttc cgctggattc ccgccggaga gattctttcc
     2581 atcattcccg tgtttctgaa aatgatccaa tccggtgaaa agatcggcgg cgtggtcgaa
     2641 gacggcggcc gctggtttga tttggggaat ccggagtcct acgtccgcac ctgccggacg
     2701 atggccgatc ccgaaaacgc cccggcttac ccgctccact ggcccatgcg ggccattcac
     2761 ctcgaagcgg acatcgccgc ggatgcccgc ttcgagggca tttgcagcgt cggcgcgcga
     2821 tcccgcatcg gcccggacac ccggctcacg gatacgtttg tctgggatcg cgccaccctc
     2881 gcggaccatt cccgcttccg cggctgcatc gtgcgggatg gccggcaggc gggtggaaat
     2941 caagaggact gcattccatg atcaacggct cgatcgtctc cgccacgcgg cggcaatttc
     3001 cccatttcac gaaccaggcg ctggaggtga ttcccctcga aaagggcggt tcggatcgga
     3061 aatttcaccg catccgcacc ggggaagcgt ccatgatctt cattcaatac agcgattccc
     3121 gggaggaaaa ctcccactac gtggaaatcg cccgcttcct gcgttcgcag ggtttgcggg
     3181 tgcccgaggt ttaccaccac gacgccaaat ccaagatcat ctggatgcag gatctcggcg
     3241 aggtggacct gtggcatttc cgccagcagc cctggccggt tcgccgcccg cattacctca
     3301 gcaccctgga gcaagccgcc cgcctgcata ccacggccac cgccgcccgg cccggcgcgc
     3361 ccattcaact gcaacccgcg tttgacgaag cgctctaccg ctgggagcag gcgtatttct
     3421 ttgaaaactg cctgcagctg gcgctgggtt ttgccgaggc cgaagcccgg aggcaggcgg
     3481 aaaatcccgc gtttgcccgc attgcggccc ggctggccgg acgggccccc accctcgtgc
     3541 accgcgactt tcagtcccaa aacatcatga tctgccagga tcaggcgtgg ctcatcgatt
     3601 tccagggcat gcgccccggc ctgccgcaat acgatctcgc ctccctgctc tacgacccgt
     3661 acgtcacgct caccggggag gaacggcggg atttgctggc cgcctaccag gcatttgccg
     3721 gggtgggcgc cggtccggat tttgaggaaa tcttcgatct ctgcgcgctc caacggctca
     3781 tgcaggccct cggcgcctac gggtttctcg gcctcaaaag gggcaaaccc cactttctca
     3841 accacatccc cgccgcgaaa aaatccctgg ccgaagtcgc cgcgcgcatt gccggactcg
     3901 gggactttgt ggcattgcta agacagtcgt gaatcccaaa ccgcaaaccg tcgtcatcga
     3961 acggccgtct ccccagctgg atggtggtcg ttatcccatc aaacgaatcg ccgggcagga
     4021 tctccggatc gaagccgaca tcttcaagga tgggcacgac gtcgtccgcg ccctggccaa
     4081 atggcgccgg gtcggcgaaa cgaaatggca cgcgaccgag atgcagccgc tcatcaacga
     4141 ccgatgggag ggcgtcctcc cgctcgctga gatgggcctc tacgaataca cgatcgaagc
     4201 ctggggcgac agcttcgaga gctggcggca ggaattcatc aaaaaaagcg atgccggcat
     4261 caccgaactg acgagcgaaa tcctcaccgg ggccgccctg gccgaagccg ccgccgtgcg
     4321 cgcgaaggga accgcggacg ccaagccgct caaggagcat ggcaaaaaac tcaaggccgc
     4381 cgacgccgcc caagtgcggc aatggctcga cgcgcccgac tttctggcat tgatgcgcca
     4441 ttggccggac cggtcgttct cgacggaatt caagccctac ctccaggcgc gggccgaacg
     4501 caagcgcgcg gctttcgggg cgtggtatga attcttcccg cgttccgcgg aaggcaaggc
     4561 cgatgccggt tcgacgtttc gcgactgcct gccgcgcgtg gaggacgcgc aggccatggg
     4621 cttcgacgtc atctattttc cgccgattca ccccatcggc gtaaagaacc gcaagggccg
     4681 gaataattcg ctcacgcccg agccgggcga gcccggcgtg ccttacgcta tcggcaaccg
     4741 cgagcagggc gtcaacggcg gcgggcattg cgacgtcgcg ccggaactcg gcacgctgga
     4801 ggacttcgac tggctggtcg gcgaaatccg gaaacgcggc atggaggtcg cgctggattt
     4861 cgccatccaa tgctcgcccg accatccgtg gctgcgcgat cacccggagt ggttcttcca
     4921 ccggccggat ggcaccatca aatacgccga aaacccgccg aagaaatatg aggacattta
     4981 tccgatcaat ttccacaacg cgaactggca ggggctctgg aacgagctgc gcgacacgtt
     5041 ccggttctgg tgccgccacg gcgcgcgcat ctttcgcgtg gacaatccgc acaccaaacc
     5101 cgtcgcgttc tgggagtggg tcatcgccga ggtgcagcgc gaatttcccg acgccatctt
     5161 cctgagcgaa gcct
//
LOCUS       DATDAY010000010         3671 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_318926, whole genome shotgun sequence.
ACCESSION   DATDAY010000010 DATDAY010000000
VERSION     DATDAY010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 3671)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3671)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3671
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_318926"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2220
                     /locus_tag="VIS74_00195"
     CDS             <1..2220
                     /locus_tag="VIS74_00195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5791683.1"
                     /translation="EVDMNFMAWYWRAQPELDRLRALSPQTYLDFAITMVADQERFLS
                     ALSNGNTEKRWSDMGYVFRQATMPLPLISSISARGEYAEELQMLRRSLISLNDRIIAQ
                     GKQTNDADLANRMRVLRCLLASWWFDQPSEIHKELLRIYRDAQQSDKGLEEMIADALF
                     IASEKGYPSDLWPGPAPFRMMWKELARTLSESSHPGERFLGAAMRARGHFSLGRSEED
                     YQEALRRFPAVYATTTGEPPPGRMTFLGSSYSSILGEVFSQAAALGLGGIFENFSFFA
                     RSKTGQILRSGGYKKDIPERSRYLADLGLIRLRNIEQHGNGGLSDALWSSSRCLSSGD
                     LEFSPETLTQLSDAFDKCRRRISTVSNPSENQKRLLREIASWAKKVDGQLAGSAPQKA
                     PLLKVSQLRIPIMDGKFVASNPSPSAVFQGFNDKPAWHGGKLWMFIYYLTAGEGPAAS
                     PCYLMEYDLKTRSAVMHPTVVDSYHPLAVTDDHVICLSNYAHKILVGIYDRKTHKWEQ
                     RTLLENSKGLEMTAVGNSLVIAFEEASDGNSKGSPRGILVHDIRKNHTDLLASTSRKP
                     PASPLDDPGLAASLGVRKDSKGRVYFGKGRDGKHVAYDLQAKEWIPIENSEAGQFTAM
                     GYEERMAIEGREFYFAYKTRENLQLRLCWTSGTAGKAGYADLPVDYDFSVDETALKRF
                     PHAYDKYQFIKNNRDAFVVYTVPAGAFLANDGHFFFLSSEDMEGAIQKALGENGAAK"
     gene            complement(2387..>3671)
                     /gene="pabB"
                     /locus_tag="VIS74_00200"
     CDS             complement(2387..>3671)
                     /gene="pabB"
                     /locus_tag="VIS74_00200"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR00553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008153 - para-aminobenzoic acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HEY5791684.1"
                     /translation="SLQAAPVNLRALPSWSWEPAELAATLRDQPGFVFLDSSLTRKGA
                     ISILAWNPAEILEGRMPGTDRLDAALRQYSIESPDMGVPLGGLIGYVEFDGRYCFGVY
                     PRLLVFRHEDRSWWRTGELPPLPSAPLRPRSPQIPAPLRFQPETEREAYLARIRRIQE
                     YIAAGDIYQACLSHRWSSPFRGNAWDFYEHLRAASPAPHSAYLHFPGRRILSSSPELF
                     LRMSDREIVTRPIKGTRPRFRQLEQDQRSAFQLQTSEKEVAELVMITDLERNDLGKIC
                     DYGSVQVADLLRLEAYEQVFHLVSTVRGRLRPALAHTRALAACFPGGSISGAPKKRAL
                     EIIRELETVPRGVYTGAIGYLGFNGESQFNIAIRTVTVENQTAHFHAGGGIVADSIPE
                     KEWQETLDKAAGILAAAENFNRSLPRRKRASSAAD"
BASE COUNT          764 a         1051 c         1149 g          707 t
ORIGIN      
        1 gaggtcgata tgaacttcat ggcttggtat tggcgcgctc agccggaatt ggatcgcctg
       61 cgcgccctct caccccagac ttaccttgat tttgcgatca ccatggttgc cgaccaggaa
      121 cgcttccttt ccgcactttc aaatggaaac accgagaagc gctggtcgga tatgggatac
      181 gtcttccgcc aggcgaccat gccgcttccg ttgatttcct cgatctccgc gcgtggggaa
      241 tatgccgaag agctgcaaat gctgcgcagg agcctcattt ccctcaacga ccggatcatc
      301 gctcaaggaa aacaaacaaa cgacgccgat ttggccaatc gcatgcgggt gctcaggtgc
      361 ctgctggctt cctggtggtt tgatcagccg tccgaaatcc acaaggaact cctgaggatc
      421 taccgcgacg cacaacagtc ggacaaaggt ttggaagaga tgattgcgga cgccctcttt
      481 attgcctccg agaagggtta tccgagtgat ctgtggccgg ggccggcgcc ttttagaatg
      541 atgtggaagg aactcgcccg caccctttcg gaatcttccc accccgggga acgctttctc
      601 ggcgcggcga tgcgggccag aggccatttt tctttgggcc ggtcggagga ggattaccag
      661 gaagccttgc ggcgttttcc cgccgtttac gcgacgacca ccggggaacc gcctcccggc
      721 cgcatgacct tcctgggaag cagctattcg tccatattgg gcgaagtctt ctcccaggca
      781 gccgcattgg ggctgggggg catttttgag aacttctcct tctttgctcg ttccaagacg
      841 gggcagattt tgcggtccgg cggatacaag aaggacattc cggaacggag tcgctatctc
      901 gcagacctgg gcctgatccg cctgcggaat atcgaacagc atggcaatgg aggcctgagc
      961 gatgccctct ggtcgtcaag tcgttgttta tcaagcggcg atctggaatt ctctcccgaa
     1021 acgttgactc agctctcgga cgctttcgac aaatgcagac gccggataag cacggtttcc
     1081 aatccctcgg aaaaccagaa acggttgctg agggagattg cctcgtgggc aaaaaaggtg
     1141 gacgggcagc ttgccggatc ggccccacaa aaggctccgc tcctgaaggt gagccagctt
     1201 cgcatcccga tcatggatgg aaaattcgtc gcgtccaatc cgtctccgtc cgcggtattc
     1261 cagggtttta atgacaagcc cgcgtggcat ggaggcaagc tttggatgtt catctattac
     1321 ctcaccgccg gggaagggcc ggcggcgagc ccctgctacc tgatggaata tgacttgaaa
     1381 acgaggagcg ccgtgatgca tccgaccgtc gtggatagct accatcccct tgccgttacc
     1441 gatgatcacg tcatctgcct aagcaactac gctcacaaaa tcctggttgg aatctacgat
     1501 cgaaagactc acaaatggga acagaggacg cttctcgaaa actcaaaggg gttggaaatg
     1561 acggcggtcg gcaactctct cgtcattgcc ttcgaggaag cctccgacgg aaactccaaa
     1621 ggcagtcccc gaggcattct cgtccacgac atcagaaaaa atcatacgga tcttttggcg
     1681 agcacctccc ggaagccgcc ggcaagtccc ctggacgatc ccgggttggc ggcgtcgctc
     1741 ggggtgcgca aagattcgaa aggacgagtt tattttggca aaggcaggga cgggaaacac
     1801 gtggcctacg acctgcaggc gaaagaatgg attccgatcg aaaactcgga ggccggccaa
     1861 tttacggcga tgggctacga ggaaagaatg gccatcgagg ggcgggaatt ttattttgcc
     1921 tacaagaccc gggaaaatct gcaactgcgc ctctgctgga cctcagggac ggcggggaaa
     1981 gccgggtatg cggacctgcc cgtcgattat gatttttcag tggatgaaac cgcgctgaag
     2041 agatttcccc acgcctacga caagtatcaa ttcatcaaga acaaccgcga cgctttcgtg
     2101 gtctataccg tgcccgcagg cgcctttctg gcgaacgatg gccacttttt cttcctgtcg
     2161 tcggaggaca tggagggcgc gatccaaaag gccctcggag aaaacggggc ggcaaaataa
     2221 gaacaacgtg cgaagatcgg gttccggaaa caaccagagc cttttcggtt taatggacgc
     2281 atttccccgg cggggttggc aaccccgccc ccaccgcgaa ccggtctcct ggcggggcgg
     2341 cgttgccaac cccgccgctc atggcggcaa ctgagtcgaa gaaggtctaa tccgccgcgg
     2401 atgaggcgcg tttgcgccgg ggcagggaac ggttgaaatt ttccgccgcc gcgaggatgc
     2461 ccgccgcctt gtcgagggtt tcctgccatt ccttctcggg gatcgaatcg gcgacgatgc
     2521 cgccgccggc atggaaatgc gcggtctggt tttcgaccgt caccgtccgg atggcgatgt
     2581 tgaactggct ctcgccatta aagccgaggt agccgatggc gccggtatag acgccgcgcg
     2641 gaacggtttc gagctcgcgg atgatttcca gcgcgcgctt tttgggggcg ccgctgatgg
     2701 agccgccggg gaaacacgcc gcgagggccc gcgtgtgcgc gagggccggg cgcagacgcc
     2761 cccgcacggt ggagacgaga tggaacacct gctcgtaggc ttcgagccgg aggagatcgg
     2821 cgacctgcac ggaaccgtaa tcgcagattt tcccgaggtc gttgcgctcg aggtcggtga
     2881 tcatgaccag ctccgcgact tccttttccg aggtttggag ctggaacgcg gagcgttgat
     2941 cctgctccag ctggcggaag cgcgggcgcg tgcccttgat cgggcgggtg acgatttcgc
     3001 gatcgctcat ccgcagaaac agctccgggg aggaggaaag aatccggcgt ccgggaaaat
     3061 ggaggtaagc cgagtgcggc gcgggggagg cggcgcgcag gtgttcgtag aagtcccagg
     3121 cattcccccg gaacggactg ctccagcgat gcgacaggca tgcctggtag atgtcccccg
     3181 cggcgatgta ttcctgaatc cggcggatgc gagccaggta agcttcccgt tcggtttccg
     3241 gctggaagcg cagcggcgcg gggatttggg gagaccgcgg ccggaggggc gcggacggaa
     3301 gcggcggcag ttcgcccgtt cgccaccagc tgcgatcctc gtgccggaag acgaggagac
     3361 gggggtaaac cccgaaacaa taccggccgt cgaattcgac ataaccgatg aggccgccca
     3421 gcggcactcc catgtccggt gattcgatgg aatattgccg gagcgcggca tcgagccggt
     3481 cggtgccggg catccggccc tccaggattt ccgcgggatt ccaggcgaga atcgaaatgg
     3541 ctcccttgcg ggtgagcgat gaatccaaaa atacgaaccc cggctgatcc cgcagagtgg
     3601 cagcgagttc ggccggctcc caggaccagg atggaagtgc gcgcaaattc acgggtgccg
     3661 cttgcaaact g
//
LOCUS       DATDAY010000011         2542 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_2611223, whole genome shotgun sequence.
ACCESSION   DATDAY010000011 DATDAY010000000
VERSION     DATDAY010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2542
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2611223"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..617)
                     /gene="tmk"
                     /locus_tag="VIS74_00205"
     CDS             complement(<1..617)
                     /gene="tmk"
                     /locus_tag="VIS74_00205"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006983245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEY5791685.1"
                     /translation="MTKRGIFISFEGGEGCGKSTQIARLGQALREARREVVLTREPGG
                     TAVGEQIRHVLQYSKESSRMTPEAELLLFAASRAQLVREVIQPALAAGRVVLSDRFLD
                     STTVYQGAARRIPAETVAGINQFAIAGLLPDATLVFDLDVAVGRARMRARAGAPAHDR
                     MENEPVEFYEAVRAGYRELARSQPERLILLDAARSPDEVYAELISR"
     gene            650..1669
                     /locus_tag="VIS74_00210"
     CDS             650..1669
                     /locus_tag="VIS74_00210"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY5791686.1"
                     /translation="MKKLKIYISSAAASEKHLLEEMLPDFTLEFVDSLRDVPRTARMI
                     SIFFRDEVNEAFLDAHPSLRVINSRSTTLDHVDLKACAKRRILVRNVPTYGENTVAEH
                     TFALLLAVSRRLRECYEAVGSGRFNAENLRGFDLRGKTIGIIGAGRIGLQVIRIAKGF
                     GMRVLAYDEAPKAFYTELMDFTYVPPGRLLAESDVLSLHVPLTETNHHWLNRERLEQC
                     KPGVVIINTARGALVDTEALLEALESGQVAAAGCDVLEEERVFQGGVMNVLGIQIAER
                     LQAAAGHGGEKRLAEIQKLWRNSALLKHPRVVFTPHVAYNSDEAIRRLCEVTASQIRE
                     FAAGA"
     gene            1711..2121
                     /locus_tag="VIS74_00215"
     CDS             1711..2121
                     /locus_tag="VIS74_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HEY5791687.1"
                     /translation="MNYWMVKQEPEAYSWETFVKDKGAAWTGVRNFQARNNLRAMRKG
                     DPVLYYHSVSEKAVVGIAQVAKEAYPDPTATEGDWSCVDLKPRKPLAGPVSLEVIKAS
                     RELAGIPLVKQSRLSVLPLTEAHFREILRLGGVK"
BASE COUNT          461 a          863 c          754 g          464 t
ORIGIN      
        1 cgggaaatca gttcggcata tacttcgtcc ggcgaacgcg cggcgtcgag caggatcaat
       61 cgctccggct gcgagcgggc gagttcccgg taaccggcgc gcaccgcctc gtaaaactcc
      121 accggctcgt tttccatccg gtcatgcgcc ggcgccccgg cccgcgcgcg catccgggcc
      181 cggccaaccg ccacgtcgag atcgaagacc agggtggcgt ccggcagcag tcccgcgatg
      241 gcgaattgat tgatcccggc cacggtttcg gccgggatgc ggcgcgccgc cccctgatac
      301 accgtggtcg aatccaggaa acgatccgag agcaccaccc ggcccgcggc cagggcgggc
      361 tggatcacct cacgcaccaa ttgcgcccgg ctcgccgcga acagcagcag ttccgcctcc
      421 ggcgtcatcc ggctcgattc cttggaatat tgcagcacgt ggcggatttg ctcgcccacg
      481 gcggttccgc cgggttcgcg ggtcagcacc acctcccgcc tggcctcccg gagagcctgg
      541 ccaagccggg cgatctgcgt gcttttgccg cacccctccc cgccctcaaa gctgatgaat
      601 attcctcgtt tcgtcatggg tttgcgctag gattaagggt ggaagcccaa tgaaaaagct
      661 gaaaatttac atttccagcg ccgccgccag cgaaaagcac ctgctggagg aaatgctgcc
      721 ggattttacc ctggaatttg tggactcgct ccgcgacgtg ccccggaccg cgcggatgat
      781 ctccattttc tttcgcgacg aagtgaacga ggcctttctc gacgcccatc cgtcgcttcg
      841 cgtcatcaac agccgctcca ccaccctcga ccacgtggac ctgaaagcct gcgccaaacg
      901 ccggatcctc gttcgcaatg tgcccacgta tggcgaaaac accgtggccg aacacacctt
      961 cgcgctgctc ctggccgtct cccgccggtt gcgggaatgc tacgaggccg tgggcagcgg
     1021 gcggtttaat gccgaaaatc tgcgcggctt cgacctgcgc ggaaaaacga ttgggatcat
     1081 cggcgccggc cgcatcggcc tgcaggtcat ccggattgcc aagggtttcg gcatgcgcgt
     1141 gctcgcctat gacgaagcgc cgaaggcgtt ttacacggag ctgatggatt tcacctacgt
     1201 gccgcccggc cggctgctgg cggagtccga cgtgctcagc ctgcacgtgc cgctgaccga
     1261 aaccaaccac cattggctca accgggaacg cctggagcaa tgcaaacccg gcgtggtgat
     1321 catcaacacc gcgcgcgggg ccctcgtgga cacggaagcc ctgctcgaag ccctggagag
     1381 cggccaggtg gccgcggccg gctgtgacgt gctggaggag gaacgcgtgt ttcagggcgg
     1441 cgtcatgaac gtgctcggca tccagatcgc ggaacggctc caggccgccg cgggccacgg
     1501 cggggaaaag cggctggcgg aaatccagaa gctctggcgg aacagcgccc tgctcaagca
     1561 cccccgcgtc gtgttcacgc cgcacgtcgc ctacaacagt gatgaagcca tccgccgcct
     1621 gtgcgaggtg acggcgtccc aaatccggga attcgcggcc ggcgcgtaat ttttctcact
     1681 tgatcgcgcc ctctcattta taaaccgccc atgaattact ggatggtcaa acaggaaccc
     1741 gaggcctact cgtgggaaac cttcgtgaag gacaagggcg ccgcctggac cggcgtgcgg
     1801 aattttcagg cccggaataa tctgcgcgcc atgcgcaagg gcgatcccgt gctgtattat
     1861 cacagcgtga gcgaaaaagc cgtggtcggc atcgcccagg tggcgaagga ggcgtatccc
     1921 gatcccaccg ccaccgaggg cgactggagc tgcgtggacc tgaagccccg caagccgctg
     1981 gccgggccgg tttcgctgga agtcatcaag gccagccgcg agctggcggg catcccgctc
     2041 gtcaaacaat cccgcctcag cgtgctgccg ctcaccgagg cgcattttcg ggaaatcctg
     2101 cgcctcggcg gagtgaaata agccgttctc aaaaggggcg aaacaccaaa cgcgttgtct
     2161 cccgccgctt ggcttttcgc caccggagca gacctccccg cttaccctgt catcacttcc
     2221 cgaccgtcac ccacgcagac cccgccagcc cattgcgaaa accccgacga ttgctccggt
     2281 tctcgctctg cggccttcgc ccactattga agggctcggc tgccggttgt tttccttttc
     2341 gaggctcatc tttgtgattt actttcgttg taacctgccg gttcgactcc atgcctgctc
     2401 cccaccccgt ctcacgacga cgcagttggc acggtcttcg gtgctgaacc gtctaattgc
     2461 accggcggga cttccacccg cgttgacgcc cgcttcacgg gcgcactatt ggttggcctg
     2521 tacgtcacat cgtcaaatta tt
//
LOCUS       DATDAY010000012         2259 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_2610405, whole genome shotgun sequence.
ACCESSION   DATDAY010000012 DATDAY010000000
VERSION     DATDAY010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2259)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2259)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2259
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2610405"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..682)
                     /locus_tag="VIS74_00220"
     CDS             complement(<1..682)
                     /locus_tag="VIS74_00220"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HEY5791688.1"
                     /translation="MRKNQKWLLALQLLVTGGLLAWIFSQREFRNQIGEVLLNEDPSW
                     LLAGFLLAGASNLLGAVRWWIFLITLEIRIRFGHALRIYFLGLFFSTFLIGMVGGDAA
                     RALILIGQGHRRSSAFLSVVLDRISGLVVLVLSSVVLMLTHYDWLAGSRVVERMMHVI
                     LLYFGALTVLLILSFVSVRMKLPDRIPDWVSFKWRIQEFCRGYYLFVSRWKNALLACG
                     VSLGVTLLY"
     gene            759..1613
                     /locus_tag="VIS74_00225"
     CDS             759..1613
                     /locus_tag="VIS74_00225"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEY5791689.1"
                     /translation="MEKGLFHTAKNYLIQMQSLAIFFGIGLGVTVGHLFWQRLLRRPV
                     SRDAGRKLLGNLFHYYVNRLRRSGQFEIWIDPRLRAFRSERRLILAGNHPSLLDVVMV
                     ISELREATCIIRGNLLRNPSMGGGALMAQYVTNDSGPGLIRQGREKLLNGETLLIFPE
                     GTRTIFPAPVNPFKKGCALLAAATGAEVQTLLIEQEGRYLGKRARLLRPFPAPVRFRV
                     HAGERFRKEPDEDVRDFSDRLEAYFLKNLKHTETGIYLAGPSSFIHSADSAHASREPD
                     SCHPSPQL"
     gene            1567..>2259
                     /locus_tag="VIS74_00230"
     CDS             1567..>2259
                     /locus_tag="VIS74_00230"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEY5791690.1"
                     /translation="MRAASPTHAILLPSYNSGEQLSRTLEKILPLWRPVIVVVDGSTD
                     GSGQAARARFGGEPDCHFLMLARNRGKGGAAAEGFRLAERLGLTHVLVMDADGQHSEK
                     SIAPFMQASFLHPHAMILGVPVFGPDAPAERVRGRAVGNWWANLATWWGGIHDSLFGL
                     RVYPVKPARRIMDAIRTARRFDFDTELAVRLYWAGIAPISLPAPVRYPPATEGGVTHF
                     RYLRDNLLLAGTH"
BASE COUNT          451 a          757 c          655 g          396 t
ORIGIN      
        1 aatacagcag cgtgacgccc agcgaaaccc cgcaggccag gagcgcgttt ttccatcggg
       61 aaacgaacag gtaataaccg cggcaaaatt cctgaatccg ccacttgaag gaaacccagt
      121 cgggaatgcg gtccggcagc ttcattcgca cggaaacaaa cgacaggatc agcagaacgg
      181 tcagcgcccc aaaatacagc agaatcacgt gcatcatccg ctccaccacc cggctgcccg
      241 ccagccagtc gtaatgcgtg agcatcagca ccaccgaact gagcaccagc acgaccaggc
      301 cgctgatgcg gtccagcacc acggagagaa aggccgagct gcgccggtgc ccctgcccga
      361 tcaggatcaa cgcccgggcc gcgtcgccgc cgaccatgcc gatcaaaaac gtgctgaaaa
      421 acagcccgag gaaataaatc cgcagcgcgt gcccgaagcg gatccggatc tccagggtga
      481 tcagaaaaat ccaccagcgg accgccccga gcagattgct cgcgccggcg agcagaaatc
      541 ccgccagcag ccacgaagga tcctcattca gaagcacctc gccgatctga ttgcgaaact
      601 cacgctggct gaaaatccac gccagcagcc ctccggtcac cagaagttgc agcgctagca
      661 gccatttttg atttttccgc atgaaatgga aggactcccg tgtcaatgaa agcgcttttc
      721 aaggcaagcc ccgaaaacta gcatgcaggc aatccatcgt ggaaaaaggc ctgttccata
      781 ccgccaagaa ttacctcatc caaatgcaga gcctggccat tttttttggg atcggcctcg
      841 gcgtgaccgt ggggcatctc ttctggcagc ggctgctgcg ccgccccgtc tcgcgcgacg
      901 cgggccggaa gttgctggga aatcttttcc attattacgt caaccgcctg cgccgctcgg
      961 gccagtttga gatctggatc gatccccgcc tccgcgcctt ccggagcgag cgccgcctca
     1021 tccttgccgg gaatcatccg agcctgctcg atgtggtcat ggtgatttcc gaattgcgcg
     1081 aagccacgtg cattattcgc gggaatttgc tgcggaatcc ttcgatgggc ggaggcgccc
     1141 tcatggccca atacgtgacc aacgattccg gccccggcct cattcgccag ggacgggaaa
     1201 aactgctcaa cggcgagacg ctgctcatct ttcccgaggg aacgcggacg atttttcccg
     1261 cgccggtcaa tcccttcaag aaaggctgcg ccctgctcgc cgcggccacc ggcgcggagg
     1321 tgcaaacgct gctgatcgaa caggagggcc gttatctcgg aaagcgcgcc cggctgctgc
     1381 gcccctttcc ggctcccgtg cggtttcgcg tgcacgccgg cgagcgcttc cggaaggaac
     1441 cggacgagga cgtgcgcgat ttttcggatc gcctggaagc ctattttctc aaaaacttaa
     1501 aacacaccga aaccggcatt tatctggccg ggccatcctc attcatccac tctgccgatt
     1561 ccgcccatgc gagccgcgag cccgactcat gccatccttc tccccagcta taacagcggg
     1621 gaacaattga gccggaccct ggaaaaaatc ctgccgctct ggcggccggt gatcgtggtg
     1681 gtcgatggca gcacggacgg cagcggccag gcggcccgcg cgcgttttgg cggggaaccg
     1741 gactgccatt tcctcatgct ggcgcgcaac cggggcaagg gcggcgcggc ggccgaggga
     1801 ttccggctgg cggaacgcct gggcctcacc cacgtgctgg tcatggacgc ggatggccag
     1861 cattccgaaa aatccattgc tcccttcatg caggcctcgt ttctgcaccc gcacgccatg
     1921 atcctcggcg tcccggtttt cgggccggac gcgccggcgg aacgcgtccg cggccgcgcg
     1981 gttggcaact ggtgggcgaa tctcgccacc tggtggggcg gcattcacga ttcgctcttc
     2041 ggcctgcgcg tttaccccgt caaaccggcc cggcgcatca tggacgccat ccgcacggcc
     2101 cggcgcttcg atttcgacac cgagctggcg gtgcggctgt attgggcggg catcgcgccc
     2161 atcagcctgc ccgcgcccgt gcgttatccg cccgccaccg agggcggcgt gacgcatttt
     2221 cgttacttgc gcgacaatct gcttctcgcc ggcacgcat
//
LOCUS       DATDAY010000013         7181 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_314878, whole genome shotgun sequence.
ACCESSION   DATDAY010000013 DATDAY010000000
VERSION     DATDAY010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 7181)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7181)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7181
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_314878"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..268
                     /locus_tag="VIS74_00235"
     CDS             <1..268
                     /locus_tag="VIS74_00235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5791691.1"
                     /translation="VGMSTPSLYRILCANWLRPVGWSADGRDGAPGRNLDAACADILG
                     QLEPGAILLFHQGGRADRAEALEKLLRGIQAQGYRCILPPHEPL"
     gene            255..1163
                     /locus_tag="VIS74_00240"
     CDS             255..1163
                     /locus_tag="VIS74_00240"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEY5791692.1"
                     /translation="MNRSEPRPHTERLFYKVIYTTTIFRLVDWLYRWLGRRVFNAVSS
                     LIARVYARTHPEIRAMVADNLALLTGRRDDSKAREVFRNFGLAISDYVCTGNMRPADV
                     RAWVTDYRGMEHIEAAHRAGRGAILATGHFGFFELGGHFLGQRGLPVTALTLPEPSPA
                     LTQWRADFRARWGVRTLAIGADPFASLEVVRELNKGHLCAMLVDRPFDSAHRLAVSLP
                     GGRTAFSTSAALLAYLADCPLIPVATSRLPGGTYRILAKPAIRIDRSLGRQASVTQAT
                     LELAGSLFEEIQQCPTQWYQFVSVRC"
     gene            1133..1786
                     /locus_tag="VIS74_00245"
     CDS             1133..1786
                     /locus_tag="VIS74_00245"
                     /inference="COORDINATES: protein motif:HMM:NF015510.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HEY5791693.1"
                     /translation="MVSICLRPLLILLALSAGLAAAEMSPAEIEALLQKIPAGQARSL
                     DFQETKKMRLLKDPVVTEGKLTVAPPGKVRREIRKPSPSLSVSDGATLWLYYPEFAEA
                     EKYDLSKNSPISKMIEAITASFDLGKLRELFDLRGERLTDGYALTLQPRNSALRKNVR
                     ELRLSLSPELKLARSRLIGKDGSETVTVYRNEKILPPAAGTFTFTPPPGVKVTEPLR"
     gene            complement(1984..3762)
                     /gene="aspS"
                     /locus_tag="VIS74_00250"
     CDS             complement(1984..3762)
                     /gene="aspS"
                     /locus_tag="VIS74_00250"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF001750.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEY5791694.1"
                     /translation="MSYRSLDCGALRAGDADREATLCGWVDSRRDHGGVIFIDLRDRE
                     GITQVVFRPEEQAAAAAAAHGLRAEDVIQITGKVVPRLAGTENPKLPTGAVEVVASRL
                     TILNKSDVLPFQLDARVANEDLRLEYRYLDLRRPEMAKNLKLRHRVTKTARDYFDEQG
                     FLEVETPILSNPTPEGARDFLVPSRLTPGSFYALPQAPQQYKQLLQVAGLEKYFQIAR
                     CFRDEDQRAERQLEFTQIDVEASFTTEREIQTLIEGLLVRIFREAKGVEIPAPFPRMP
                     YQEALDRFGSDKPDLRFGLEIVELSDIFRDSQFKVFRSVLDGGGVIRALNAKGAAEHF
                     SKEQLKKWEEWAKTELGAKGLAYIKAGPSGEWESPIVKFFSEAEKAALAERLGFAAGD
                     VLFFGADQWAGVCDVLGRVRLRCAEFLGLTKGNDSFHFLWVVDFPLLAFVSDENKWVA
                     VHHPFTRPKDEDLPLLEAGDYGKVRAIAYDVVLNGVELGGGSIRIHEKELQAKAFAAL
                     GIGPEEQREKFGHLLRAFSFGAPPHGGLALGLDRIIMLLAGTESIRDVIAFPKNNRGQ
                     DLLTHSPVKVDFKQLRELYIQVTKKE"
     gene            complement(3769..5013)
                     /gene="hisS"
                     /locus_tag="VIS74_00255"
     CDS             complement(3769..5013)
                     /gene="hisS"
                     /locus_tag="VIS74_00255"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR00442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEY5791695.1"
                     /translation="MQTLPGFRELYPEDCARRNYLFHAWRAVARRYGFQEYDGPTLEA
                     AELYEKKNSGGEILQQLYQFEDRGGRRVALRPEMTPTLARMAADREKQSRKPMKWFSI
                     GPFFRHERQQKGRLREFIQLNCDLLGDSSPGADAETLALCIDLLRELGFDSNDIVLRL
                     SDRRAWTEFLKERSIAEERAGDFLAIVDKLEREPEESLREKLAEFGITLDDVRAFIAS
                     EGNGAFAALLADLEARGLRDYARVDLSIVRGLAYYTGLVFEVFDRAKKFRAVAGGGRY
                     DQLVASLSEGRSQLPAIGFGMGDVVLMNLIEATPHALARLQAGTEAAVQIYGIVADEA
                     QRGPALGLVQRLREAGWRVDFGLTPAKVGKQFQAAEASGATFAVLVGGEWPEVKVKHL
                     PSRTEQLLPQETLADWLKTAQL"
     gene            5094..5513
                     /locus_tag="VIS74_00260"
     CDS             5094..5513
                     /locus_tag="VIS74_00260"
                     /inference="COORDINATES: protein motif:HMM:TIGR00149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HEY5791696.1"
                     /translation="MKSYRKELWFDIPTRRQYRNITPDVERCLAESGIREGLCLVNAM
                     HITASVFINDNESGLHADFEKWLEGLAPEKPHSRYLHNRTGEDNADAHLKRSLMGREV
                     VVAVTGGGLDFGPWEQIFYGEFDGRRRKRVLVKIIGE"
     gene            complement(5560..6513)
                     /locus_tag="VIS74_00265"
     CDS             complement(5560..6513)
                     /locus_tag="VIS74_00265"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-enoyl thioester reductase domain-containing
                     protein"
                     /protein_id="HEY5791697.1"
                     /translation="MKAIRLHAFGSAPVIEELPEPPLPAGSLRLRMLAAPINPADLNV
                     IEGKYGRLPELPATLGNEGVGRVIEAGNGVSGFAPGDLVLPMQSGVWAEQVVVPAGPA
                     IRISGTPDPLQAAMLMVNPATAYGLLARFAALEPGDWIVQNAANSGVGRCVIQIARQL
                     GFRTLNVCRRPELLEELTALGGDECVTEEMDLKNHAPAAKLALNAVGGASALNVANAL
                     APGGAHVTYGAMGRQPVKIPNGLLIFKNLSFHGFWVTKWLAALEPAARRALFDRLAGW
                     VSAGRLIQPIAGIYPPEKIAEALAEAAREKRSGKVMLKFAD"
     gene            complement(6510..7070)
                     /locus_tag="VIS74_00270"
     CDS             complement(6510..7070)
                     /locus_tag="VIS74_00270"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY5791698.1"
                     /translation="MRAILLDGLWGYGEGRFRPMARAFERHGASRIEIFRYDCSGRSS
                     FESLAREFTAFVRRSPEPAVVLAHSMGGLVLRTALLHEPALPIRRVAFINVPHTGSWA
                     GWLLPFAGIRQMRPGSAFLRRLADQAWEIPSLAIWCPGDLMVLPGSSARWARAHSTEC
                     CWVPAHIWPMVSPGWQKRIARFLFQT"
BASE COUNT         1284 a         2367 c         2258 g         1272 t
ORIGIN      
        1 ggtgggcatg agcacgcctt cactgtatcg aattctctgc gcgaactggc ttcgccccgt
       61 gggctggtcg gcggacggac gcgacggcgc tcccgggcgg aatctggatg cggcctgcgc
      121 ggacattctc ggccagcttg aacccggtgc aattctgctt ttccatcagg gcggacgggc
      181 ggaccgcgcc gaggcgttgg aaaagctgct ccgcggaatc caggcgcagg gctaccgctg
      241 tattctcccg ccgcatgaac cgctctgagc cgcggccgca caccgagcgg ttgttttaca
      301 aggtcatcta taccaccacg atcttccgcc tggtggactg gctttaccgg tggctggggc
      361 ggcgggtttt taatgccgtg agcagcttga tcgcccgggt ttatgcgcgg acgcatccgg
      421 aaattcgcgc catggtcgcg gataatctcg ccctgctcac cggccggcgc gatgactcga
      481 aagcccgcga ggtttttcgc aatttcggcc tggccatttc cgattacgtc tgcacgggga
      541 acatgcggcc ggcggacgtg cgcgcgtggg tcacggacta ccggggcatg gaacacatcg
      601 aggccgccca ccgcgcgggc cgcggcgcga ttctcgccac cggccacttt ggcttcttcg
      661 agctcggcgg ccattttctg ggccagcgcg gccttcccgt caccgcgctg accctgcccg
      721 agccctcgcc ggcgctcact caatggcggg cggatttccg cgcgcgctgg ggcgtgcgca
      781 cgctggcgat cggcgccgat cctttcgcct cgcttgaagt ggtgcgtgaa ttgaacaagg
      841 gccacctctg tgcgatgctg gtggatcgcc ccttcgactc cgcccaccgc ctggcggttt
      901 cgctgccggg cgggcgcacg gccttttcca cctccgcggc gttgctggcc tatctcgcgg
      961 actgccccct cattccggtc gccacctcgc ggctgcccgg cggaacctac cggatcctcg
     1021 ccaaaccggc catccggatc gaccgttcgc tcgggcggca ggcatccgtc acgcaggcga
     1081 cactcgaact ggctggatcg ctcttcgaag aaatccaaca atgccccacg caatggtatc
     1141 aatttgtctc cgtccgctgc tgatcctcct ggcgctttcc gccggcctgg ccgccgcgga
     1201 aatgagcccg gcggaaatcg aagccctgct gcaaaagatt cccgccgggc aggcgcgctc
     1261 cctggatttt caggagacca aaaaaatgcg cctgctcaag gatcccgtcg tcacggaggg
     1321 gaaattgacc gtggctccgc cgggaaaagt ccgccgcgaa attcggaaac cctcgccctc
     1381 cctttccgtg agcgatggcg ccacgctctg gctgtattat ccggaatttg cggaggcgga
     1441 aaaatacgac ctgagcaaaa acagcccgat ctcgaaaatg atcgaggcca ttacggcgag
     1501 cttcgacctg ggcaagctcc gcgagctgtt cgacctccgg ggtgagcgcc tcacggatgg
     1561 ctacgcactc acgcttcaac cgcgaaactc cgccctgcgc aagaacgtgc gggaattgcg
     1621 cctgagcctt tcgccggagc tgaaactggc gcgttcccgg ttgatcggca aggacggctc
     1681 cgagaccgtc accgtttacc ggaatgaaaa aatcctcccg ccggctgcgg gcactttcac
     1741 cttcacgccc ccgccgggag tgaaggtcac cgaaccgctg agataggcgc ttcttgatct
     1801 gatttatccg cggatttcgc ggatttcgcc gattattgag tttaggagat ttgatcgcgg
     1861 tggacgcgcc tcccttggcg gtgttggcaa caccgcccta ccgtcaatcc agtccgcggt
     1921 agggtggcgt tgccaacgcc accgggcatc gctctcaaaa atccgtattc cgcaatcccg
     1981 cttttactcc ttcttcgtga cctggatgta aagctcgcga agctgcttga aatcgacctt
     2041 cacgggcgag tgggtcagca gatcctggcc gcggttgttt ttcgggaagg cgatgacgtc
     2101 gcggatggat tccgtgccgg cgagcagcat gatgatacga tccagcccca gcgccagccc
     2161 gccgtgcggc ggggcgccga aggaaaacgc ccgcaagaga tggccaaatt tttcccgctg
     2221 ttcctcgggg ccaatgccca gcgcggcgaa ggctttcgcc tgcaattcct tctcgtgaat
     2281 ccggatcgaa ccgccgccca gttccacgcc gttgagcacg acatcgtagg cgatggcgcg
     2341 gactttcccg tagtcgccag cttccagcaa gggcagatcc tcgtccttcg ggcgggtgaa
     2401 gggatggtgc accgcgaccc atttgttttc gtcggacaca aaggccagca gcgggaagtc
     2461 caccacccag aggaagtgga acgaatcatt gcctttcgtc agtccgagaa attcggcgca
     2521 gcgcaagcgg acccggccaa gcacgtcgca gacgccggcc cattggtccg cgccgaagaa
     2581 gagcacgtcg ccggccgcga agccgagccg ctcggccagc gcggcctttt cggcctccga
     2641 gaaaaatttc acgatcgggc tttcccattc gccgcttggc ccggccttga tgtaagccag
     2701 ccccttcgcg ccgagttcgg tcttggccca ttcctcccac ttcttgagct gctccttgga
     2761 aaaatgctcc gccgcgccct tggcgttgag cgcgcggatg actccgccgc cgtcgagcac
     2821 cgagcggaaa accttgaatt gcgaatcgcg gaaaatgtcg ctgagttcca cgatctcaag
     2881 gccgaaacgc aggtcgggct tgtcggaacc gaagcgatcc agggcttcct gatagggcat
     2941 gcgcggaaag ggcgccggga tttccacgcc cttggcctcg cggaaaatgc gcacgagcag
     3001 gccttcgatc aaggtctgga tttcccgctc ggtcgtgaac gaggcctcga cgtcgatctg
     3061 ggtgaactcc agctgccgtt cggctcgctg gtcctcgtcg cggaaacacc gcgcgatctg
     3121 gaaatatttc tccaggcccg cgacctgcag gagctgcttg tattgctgcg gcgcctgcgg
     3181 caatgcgtaa aacgagccgg gggtcaggcg cgagggcacc agaaaatcgc gcgcgccttc
     3241 cggcgtggga ttcgacaaaa tcggcgtctc cacctcgaga aatccctgct cgtcgaaata
     3301 atcgcgcgcc gtcttggtta cgcgatggcg cagcttcaga ttcttcgcca tttccggacg
     3361 gcggagatcg aggtagcggt attccagccg caggtcctca ttcgccaccc tggcgtcgag
     3421 ctggaagggc agcacgtcgg acttgttgag gatcgtgagc cgcgaggcga cgacttcgac
     3481 cgcgcccgtg ggcagcttgg gattttccgt cccggccagg cgcgggacga ccttgccggt
     3541 gatctgaatg acatcctccg cgcgcaatcc gtgcgccgcc gcggcggccg ccgcctgttc
     3601 ctcgggccgg aacaccacct gggtgatccc ctcccggtcg cgcaaatcga tgaaaataac
     3661 gccgccgtga tcccggcgcg aatccaccca gccgcaaagc gtcgcttcgc ggtccgcgtc
     3721 gccggcgcgc agggcgccgc aatccaaact ccgataactc atggggggtt aaagctgcgc
     3781 ggtttttagc caatcggcaa gcgtctcttg cgggagaagt tgctccgtcc ggctcgggag
     3841 gtgcttgacc ttgacctccg gccattcccc gcccaccagc acggcgaaag tcgcgccgct
     3901 ggcttcggcg gcctgaaatt gcttgccgac cttggctggc gtgaggccga aatccacgcg
     3961 ccagccggct tcccgcaggc gctggacgag gccgagcgcc ggtccgcgct gcgcctcgtc
     4021 ggccacgatc ccataaatct gaaccgccgc ctccgtgccc gcctgcaagc gcgccagggc
     4081 gtgcggcgtg gcctcgatga gattcatgag cacgacgtcg cccatgccaa agccgatggc
     4141 cggaagctgg ctgcgcccct cgctcaggct cgccaccagc tggtcatacc gcccgccgcc
     4201 ggcgacggcg cggaacttct tcgcgcggtc gaacacctca aaaaccaggc cggtataata
     4261 cgccaggccg cgcacgatcg aaagatccac ccgcgcgtaa tcgcgcagcc cccgcgcctc
     4321 gagatcggcc agcagggccg cgaaggcgcc atttccttcg ctggcgatga aggcgcggac
     4381 gtcgtccagc gtgattccga actccgccag cttttcgcgg agcgattcct ccggctctcg
     4441 ctcgagcttg tccacgatgg ccagaaaatc ccccgcacgc tcttccgcaa tgctccgttc
     4501 ctttaaaaac tccgtccagg cccgccggtc gctgagccgc agcacgatgt cgttcgaatc
     4561 aaatcccagc tcgcgcagca ggtcgatgca cagcgccagc gtttccgcat ccgcgccggg
     4621 ggaggaatcc ccgagcaggt cgcaattgag ctggatgaat tcgcgcagcc ggcccttctg
     4681 ctggcgttcg tgacggaaaa agggcccgat gctgaaccac ttcatcggct tgcgggattg
     4741 cttctcccgg tcggcggcca ttcgcgccag cgtgggcgtc atttccggcc ggagcgccac
     4801 gcgccggccc ccgcggtcct cgaattgata gagctgctgg aggatttcgc cgccgctgtt
     4861 tttcttctcg taaagttcgg cggcttcaag cgtggggcca tcgtattcct gaaagccgta
     4921 acggcgcgcg acggcgcgcc aggcgtggaa caaataattc cgccgggcgc agtcctcggg
     4981 atacaattca cgaaatccgg gcagggtctg catgggtcaa acggcccagc ttcgccgttc
     5041 gccgacgttt ttcaagctgg tttaaaggcc gcggcaaact accctcggat gccatgaaat
     5101 cctatcgcaa ggaattgtgg ttcgacatcc cgacccggcg gcaataccgg aacatcacgc
     5161 cggacgtgga acgctgcctc gcggagagcg gaatccgcga gggcctgtgc ctcgtcaatg
     5221 cgatgcacat caccgcgagc gtattcatca acgataacga aagcggcctg cacgccgact
     5281 ttgaaaaatg gcttgaaggt ctcgcgccgg aaaagccgca cagccggtat ctccacaatc
     5341 gcacgggcga agataatgcc gacgcgcacc tcaagcgttc cctcatggga cgggaggtgg
     5401 tggtcgcggt aaccgggggt ggattggatt tcggcccgtg ggagcagatt ttttacgggg
     5461 aatttgacgg acggcgccgc aagcgggtgc tggtgaagat catcggcgag tgatcccgaa
     5521 cgcggaaaac gacggcggga tcggcgagcc gtccgtggat caatccgcaa atttcagcat
     5581 caccttgccg gagcgctttt cccgggcggc ttccgcgagg gcttcggcga ttttttcggg
     5641 aggataaatt ccggcaattg gctggatgag tcgccccgcg ctcacccagc cggcgaggcg
     5701 gtcgaacaaa gcccgccggg cggccggctc cagggcggca agccatttcg tgacccagaa
     5761 tccgtggaag ctcaggtttt tgaagatgag caggccgttg gggattttca ccggctggcg
     5821 gcccatggcg ccgtaggtca cgtgcgcccc gcccggagcg agcgcgttgg ccacgttcag
     5881 ggcgctggcc ccgccgacgg cattgagggc gagcttcgcc gccggggcgt gatttttcag
     5941 gtccatttcc tcggtcacgc attcgtcccc gcccagggcg gtcaattctt cgagcagctc
     6001 cggccggcgg cagacattga gcgtgcgaaa gcccagctgt cgggcgattt gaatgacgca
     6061 gcgccccacc ccggagttgg cggcattctg cacgatccag tcgccgggtt ccagggccgc
     6121 aaaccgcgcc aacaggccat aagccgtggc gggattgacc ataagcatcg ccgcctgcag
     6181 cgggtcgggg gttccggaaa tccggatcgc cggaccggcg ggaaccacca cctgctccgc
     6241 ccagacgccg ctctgcatgg gcagcaccag atcgccgggc gcaaaaccgc tcacgccatt
     6301 gcccgcttcg atcacgcggc ccacgccctc gtttcccagc gtggcaggca attcgggcag
     6361 acggccgtat ttgccctcga tgacgttgag atcggcgggg ttgatcggcg cggccagcat
     6421 tcgcaggcgc agcgagccgg cgggaagcgg cggttcgggc agctcctcga tcaccggcgc
     6481 cgacccgaaa gcgtgaaggc gaatggcctt catgtttgga ataaaaaccg cgcgatgcgt
     6541 ttctgccagc ccggggaaac catcggccag atatgcgcgg gcacccagca gcattcggtg
     6601 gaatgcgccc gggcccagcg cgcgctcgag cctggcagga ccatcagatc cccgggacac
     6661 cagatcgcca gcgagggaat ctcccaggcc tgatcggcca ggcggcgcag aaacgcggaa
     6721 ccgggccgca tctgccggat gccggcaaag ggcagcagcc agcccgccca cgagcccgtg
     6781 tgcggcacat tgatgaaggc cacgcgccga atgggcagcg cgggctcgtg cagcagcgcc
     6841 gtccgcagca cgaggccgcc catgctgtgc gcgaggacga cggcgggctc gggcgagcgc
     6901 cggacaaacg cggtgaactc gcgggcgagc gactcaaagg atgagcgccc gctgcagtca
     6961 taccggaaaa tttcgatccg ggaagcgccg tggcgctcga aggcccgggc catgggccgg
     7021 aagcggccct cgccgtatcc ccataatccg tcaagcaaga tcgccctcat gatttcagga
     7081 tgccggggtg atccgcactt cctccacccg ggtctgatcc agcaggcatt gccccatgcg
     7141 atgggcggtg gaagtccggg cttgcgggga tacgaccaaa a
//
LOCUS       DATDAY010000014         3175 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_312866, whole genome shotgun sequence.
ACCESSION   DATDAY010000014 DATDAY010000000
VERSION     DATDAY010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 3175)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3175)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3175
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_312866"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..375
                     /locus_tag="VIS74_00275"
     CDS             <1..375
                     /locus_tag="VIS74_00275"
                     /inference="COORDINATES: protein motif:HMM:NF020052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification enzyme R subunit
                     C-terminal domain-containing protein"
                     /protein_id="HEY5791699.1"
                     /translation="AEKGFGAGQLAEMQKIIEAERSDLFDVLAYVAYALPPLTREDRA
                     AKAKVEISTHFNTKQQVFLDFVLSHYVQVGVEELDQEKLTPLLKLKYHNAISDAVADL
                     GKPEEIGRTFAGFQKYLYQDVA"
     gene            complement(384..647)
                     /locus_tag="VIS74_00280"
     CDS             complement(384..647)
                     /locus_tag="VIS74_00280"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY5791700.1"
                     /translation="MPLSQREAEISWWIGEGFTDREIAILLGISPRTVHSHRDHILRK
                     LKVRTRAAIAVWIVKKYYGLLSVVWRHWRRWREVWRRRSELSW"
     gene            1132..2088
                     /locus_tag="VIS74_00285"
     CDS             1132..2088
                     /locus_tag="VIS74_00285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5791701.1"
                     /translation="MARNEIPQNYDPLVSLAEDAADGAQAHGAAIGLSQNTEAKILAD
                     LLNLTGDPNAGPPPTPGRQGEWNAAKAAKVAASGAARAAESNARAFCASAVGVLKNYL
                     GPRWNSQWEAAGFTNGSLRMPDDPLPMLGELRAYFLARAAHENAPLGVSAAACLAQVH
                     TVSAARSASNASNVAAGLAKQARDAALRALYTRMSGLLAELDQLLDDDDPRWYAFGFD
                     RPADGEQPGLALNLVLTPGGPGVVIADWDDARRAQRYRVFKQVAGADANPVQVEKCGD
                     GKRIHPHRPAEWRGGENQHRAGERRGGRAGNGGADRGAVGEV"
     gene            complement(2274..2639)
                     /locus_tag="VIS74_00290"
     CDS             complement(2274..2639)
                     /locus_tag="VIS74_00290"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEY5791702.1"
                     /translation="MSRLLLDTHALIWIVRNDAALSKSAIDAFETSEEIFCSVASFWE
                     IAIKRGKDPKSFPLPENWLELLTGEIRLNGMSLLGVGLSHCEAVERLPPHHKDPFDRM
                     LIAQARTEKLIILTKDPYF"
     gene            complement(2636..2908)
                     /locus_tag="VIS74_00295"
     CDS             complement(2636..2908)
                     /locus_tag="VIS74_00295"
                     /inference="COORDINATES: protein motif:HMM:NF014643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEY5791703.1"
                     /translation="MTQVTIHEAKTQLSRLIQKALAGEEVIIANRDKPVVRLAVIATE
                     KPKRRLGGLAREFGGFSWREWKQMDREIEKDFYRDDPNVPLRSRKP"
BASE COUNT          620 a          985 c          999 g          571 t
ORIGIN      
        1 gcggaaaagg ggtttggagc cggccagctg gcggagatgc aaaagatcat tgaggcggaa
       61 aggagcgatc tttttgacgt cctcgcctat gtggcctatg cgctgccgcc attgacgcga
      121 gaagatcgtg ccgccaaggc gaaggtggaa atcagcaccc acttcaacac caagcagcag
      181 gtatttctgg atttcgtgct ctcgcattac gtccaggtcg gcgtcgagga actcgaccag
      241 gaaaagctga cgccgctcct gaaactcaaa taccataacg ccatttccga cgccgtggct
      301 gacctcggaa agccggagga aatcggccgg acctttgccg gtttccaaaa gtatctctac
      361 caggacgtgg cttgatcctc agatcaccag gagagttccg atctccgacg ccagacttcc
      421 ctccagcgcc gccaatgccg ccaaaccacc gacagcagcc cataatactt cttcacgatc
      481 cacaccgcga tggcggcgcg ggtgcggact ttgagcttgc gcaggatgtg gtcgcggtgg
      541 ctgtgcacgg tgcggggact gatgccgagc agaatggcga tctcccggtc ggtaaagcct
      601 tcgccgatcc accaggagat ttcggcctcg cgctggctca gcggcacggg cggcgggatg
      661 ccctttgccc aaaaccgcgt ttttctcggt aaaaggcccg gcccggcagc catcggccgc
      721 caatttgctt tatcggacga ttccggcgcc cgcattgggg gccaatgggc gggtggcggc
      781 gtttcccacc gaggcattgg cggccaatgg aagaccgcga agcgtccgcg ccgggttatc
      841 gggcgtccat ggaggcgcgg gaagggttcc cggggaggca tcgggcccca atgcttctcc
      901 cggaagtccg cgcggcggcg cagtggcggc caatgacgcg gtggcggctg gcaaggaatg
      961 ggcaatggcg gccaatggcc ggatgcgcgg tctttccgcc cgccgattgg cagccgatgg
     1021 gcgattttca aggaatcttc ctcctcgggg aatgcgccgt catttcccgg caaccgaacg
     1081 gtaaaacccc aaggttttga cggcgcaaaa ccctccaaaa taccacgaac catggccaga
     1141 aacgaaattc cgcaaaacta cgatccgctc gtctccctcg ctgaagacgc cgccgacggc
     1201 gcgcaggcgc acggcgccgc catcggcctc tcgcaaaaca cggaggcgaa aatcctcgcc
     1261 gatctcctga acctcaccgg cgatccgaac gccgggccgc cgcccacgcc aggccggcag
     1321 ggggaatgga acgcggccaa ggcggccaag gtggcggcca gcggcgcggc ccgcgcggcg
     1381 gagtccaacg cccgggcctt ctgcgcctcg gcggtgggag tattaaaaaa ctacctcggc
     1441 ccgcgctgga acagccaatg ggaggcggcg ggatttacca acggctcgct gcgcatgccg
     1501 gacgatccgc tgcccatgct cggcgaattg cgcgcgtatt tcctcgcccg cgccgcgcac
     1561 gaaaacgccc cgctcggggt gagcgccgcg gcctgcctcg cccaggtcca cacggtctcc
     1621 gccgcgcgct cggcctcgaa tgccagcaac gtggccgcgg gcctggccaa gcaggcgcgc
     1681 gacgccgccc tccgggctct ttatacccgc atgagcggcc tgctggcgga gctggatcag
     1741 ctcctcgacg acgacgaccc gcgctggtat gccttcggct tcgaccgccc ggcggacggc
     1801 gagcagcccg gcctggcgct taatctcgtg ctcacgccgg gagggccggg cgtggtcatt
     1861 gcggactggg acgatgcgcg gcgggcgcag cgttatcgcg tcttcaagca agtcgccggc
     1921 gcggatgcga accccgtgca ggtggaaaaa tgcggtgacg gaaagcgaat acaccctcac
     1981 cggcctgccg agtggcgcgg cggtgaaaat cagcatcgtg ccggtgaacg acgcggggga
     2041 cgggccggaa acggcggggc agatcgtggc gccgtagggg aggtttgaag tggcatcggc
     2101 atcttgccga tgtttgggtt ggatcatcgg ctggaagccg atgccaccgc cattcgtttt
     2161 aatcggaggg cggcagtccc ttggcgccga atgatccgcc gaggaacgtc tccgcggcaa
     2221 aatcccgagc cgttccggct ttgaggtcac cacaccactt cgacatcgta ttttcaaaaa
     2281 tacgggtctt tggtgagaat gattagtttc tcggttcggg cttgggcgat gagcatgcga
     2341 tcgaagggat ccttgtggtg gggcggaagg cgttccaccg cctcgcaatg cgaaagccca
     2401 acacccaaaa ggctcattcc gttgagacgg atttctcccg tcagcaactc cagccagttt
     2461 tctggcaacg gaaaggattt cggatctttc ccgcgtttga tggcgatttc ccaaaatgaa
     2521 gccacggagc agaaaatctc ctccgaggtt tcaaaagcat cgatggcgga tttgctcagc
     2581 gcagcatcgt tccgcacgat ccagatcaac gcatgggtat ccagcagcag ccggctcacg
     2641 gcttgcggga acgaagggga acattcggat catcgcggta aaaatccttc tcgatttcgc
     2701 gatccatttg tttccactcc ctccaggaaa aaccgccgaa ttcgcgagcc agcccgccca
     2761 gccggcgctt cggtttttcg gttgcaatca ccgccagccg caccacgggc ttgtcccggt
     2821 tggcaatgat cacttcctcg ccggcgagcg ccttttggat cagccgggaa agttgtgtct
     2881 tggcttcatg aatggtgact tgcgtcatga ttttttctta gctaagaaac ttggccatgt
     2941 caaatgggcc gggaagcggc gccgaaacgc gggcgaagta gagataagca agcacccccg
     3001 cgctcacgaa aaagccgccc gcgagaaact tgcccgcctc ttgccaggga atttttttga
     3061 gcaggtgaac cccttttttg gccttttgga ttcaatcgga tgcgacggac ggctcggcga
     3121 gccgtcccta cctttaaggg gcagggcggg gtcgatgacc ccgccgtggc ttgcc
//
LOCUS       DATDAY010000015         3272 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_300445, whole genome shotgun sequence.
ACCESSION   DATDAY010000015 DATDAY010000000
VERSION     DATDAY010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 3272)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3272)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3272
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_300445"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..758
                     /locus_tag="VIS74_00300"
     CDS             <1..758
                     /locus_tag="VIS74_00300"
                     /inference="COORDINATES: protein motif:HMM:NF020296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HEY5791704.1"
                     /translation="TAFGSSEFAIREFLRLDLARTLKVMEKWSRDGGEHVRRLASEGS
                     RPRLPWSFRLEALIADPAPVAPILENLKADPSLYVRKSVANHLNDITKDHPEWVLKRL
                     TAWPRENSHTQWIVKRALRTLIKRGDRAALTVIGAGEKARVAVSHFAIEPRRIRLGES
                     VAMGFHLDSRSAQPQKLVVDYAVHYVKKSGATSAKVFKWKELTLLPGQSVTLSRRQSI
                     RNFTTREHYAGRHAVDLSINGEKLASAAFELIV"
     gene            761..1372
                     /locus_tag="VIS74_00305"
     CDS             761..1372
                     /locus_tag="VIS74_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HEY5791705.1"
                     /translation="MDCNRPASKNLEIWIENVSEFFRPLCRELRERILEWEPDLTESI
                     KWNVLCFSGRKRVCGLSGCKKHVGIALFRGVELADPAGLFTPAENNTSIRSIRITGLD
                     GFNREAFRRMLHAAMLLDADPTLPPMPPRKRDPWPAPDFFAKALKKYPAAAAGFQRLA
                     PTYQREYLIWLTTAKREETREQRLAQTLQALAAGRKWADRKPA"
     gene            complement(1495..2211)
                     /locus_tag="VIS74_00310"
     CDS             complement(1495..2211)
                     /locus_tag="VIS74_00310"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HEY5791706.1"
                     /translation="MRRLARWIFSRQTAPPPKRMGTPEDRVVVFDFDGTIADTFDAAL
                     SILNRLSGEFGYRTLQESEIPQAREMNVRQLLKFLGIPMSKMPVIAHKGVHELHSRMR
                     EIQPFPEVPGVLRELHRRGCRLGIITSNSQENVAQFLRMHELQLFDFVCTSSRLFGKT
                     REIRGVLKRFGWKAPQLHFVGDECRDIEAAKKAKILMTAVTWGYNARHVLESLKPDFV
                     IQKPHELLTLVPESAARRVD"
     gene            complement(2208..3248)
                     /locus_tag="VIS74_00315"
     CDS             complement(2208..3248)
                     /locus_tag="VIS74_00315"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY5791707.1"
                     /translation="MKYRKLRGTALEVSEVGFGLWTLSTGWWGNYTDEEAVKLMREAA
                     DLGVTLFDAADTYGNGRSEELIAQAFAGQRDRIVIATKVGYDFVNHGNERRGQREIPQ
                     DFSPAAIRRAAEAALSRLRTDVIDVLQLHNIRMEQVDDAAVWETLEDLRAEGKIRTGG
                     AALGPAIGWLGEGVRAIREHNPGILQHIYNFLEQHPGRAIQQAAEEAGARTQFFIRVP
                     HSSGMLEGKYTEDTVFEPADHRSHRPRSWLINGVRKVEQLRFLERDGRTLGQAAIQWL
                     LADGRVASVLPNIYNSEQLREFVGATDAVALTGDERARMQALYERNFGQAPEEGRYKG
                     TMELPEAAGAIA"
BASE COUNT          600 a         1070 c         1008 g          594 t
ORIGIN      
        1 tcaccgcctt cggctcctcc gagttcgcca tccgcgaatt cctgcgcctc gacttggcgc
       61 gcacgctcaa ggtcatggaa aaatggtcgc gcgacggcgg cgaacatgtc cgccggctgg
      121 ccagcgaagg cagccgtccg cgcctgccgt ggtcgttccg gctcgaggca ttgatcgccg
      181 atcccgcgcc ggtggcgccc attttggaaa acctcaaggc cgatcccagc ctctacgtcc
      241 gcaagtcggt ggcgaatcat ttaaacgaca tcacgaagga tcatcccgaa tgggtgttaa
      301 aacgcctcac cgcctggccg cgggaaaatt cgcacaccca atggatcgtc aaacgcgcat
      361 tgcggacatt gatcaaacgc ggcgaccgcg cggcgctgac ggtcatcggc gcgggcgaaa
      421 aggcgcgggt ggcggtcagc cattttgcca tcgaacccag gcgcatccgg ctcggggaga
      481 gcgtggccat gggttttcac ctcgactcgc gctcggccca accgcaaaag ctcgtcgtgg
      541 attacgcggt ccattacgtc aaaaaatccg gagcgacttc cgcgaaggtt tttaaatgga
      601 aggagctcac gctgctgccg ggacaatccg tgaccctgag ccgccgccag agcattcgga
      661 atttcaccac gcgcgagcat tacgccggac ggcacgcggt ggatttgtca atcaatggcg
      721 aaaagctcgc cagcgcggcc tttgaactta tcgtgtaggc atggattgca accggcccgc
      781 ttccaaaaat ctggagatct ggatcgaaaa cgtttccgaa tttttccggc cgctttgccg
      841 ggaattgcgc gagcggattc tggagtggga gccggacctg acggaatcca tcaaatggaa
      901 cgtgctctgc ttctccggcc gcaagcgggt ctgcggcctg agcggctgca agaagcacgt
      961 gggcatcgcc ctcttccgcg gcgtcgagct ggccgatccc gcggggctct tcacgccggc
     1021 ggaaaacaac acctcgatcc ggagcatccg catcaccggc ctggacgggt ttaaccgcga
     1081 ggcctttcgc cgaatgctgc acgcggcgat gctcctcgac gccgatccca cgctgccgcc
     1141 gatgccgccc aggaaacgcg atccgtggcc ggcgccggat tttttcgcga aggcattgaa
     1201 gaaatacccc gccgctgcgg ccggcttcca gcggctcgcg ccgacctatc aacgcgaata
     1261 cctgatctgg ctgaccaccg ccaagcgcga ggaaacgcgc gagcaacggc tcgcgcaaac
     1321 cctccaggcc ctggccgcgg gccggaagtg ggcggatcgc aaaccggcgt aagggagcgc
     1381 ttgcccaccc aaacggcacc gagcctcacg caaaggcgcg aagacgcaaa gaagcggttc
     1441 ttccccggaa atttttgcgt ctttgcgtct ttgcgtgaga tgaatttccc gggtttaatc
     1501 cacccgccgg gcggcggact caggcaccag cgtgagcagt tcgtgcggtt tttggatcac
     1561 aaaatccggc ttcaggcttt cgagcacgtg gcgcgcgttg taaccccagg tcacggccgt
     1621 catgaggatt ttggcttttt tcgccgcctc gatgtcccgg cattcgtcgc cgacgaaatg
     1681 cagctgcggc gccttccagc cgaaacgctt cagcactccg cgaatctcgc gcgtcttgcc
     1741 gaagaggcgc gaggaggtgc agacaaaatc gaacagctgc agctcgtgca tccgcagaaa
     1801 ctgcgcgacg ttttcctgcg aattggaggt gatgatgccc aggcggcagc cgcgccggtg
     1861 cagctcccgc aggacgcccg gcacctccgg aaagggctgg atttcccgca tgcgcgaatg
     1921 caattcatgg acgcccttgt gggcgatcac cggcatcttg ctcatgggaa tgccgagaaa
     1981 cttgaggagc tggcgcacgt tcatttcccg ggcctgggga atctcggatt cctggagggt
     2041 gcgataccca aattcgccgg aaagccggtt caagatgctc agcgcggcgt cgaaggtgtc
     2101 cgcgatcgtg ccgtcgaaat cgaataccac cacccggtcc tccggagtgc ccatgcgttt
     2161 gggaggaggg gcggtctggc gcgaaaaaat ccagcgggcg aggcgtctca cgcgatggca
     2221 ccagctgcct cgggaagctc catcgtcccc ttgtatcttc cttcctcggg cgcctggccg
     2281 aagttccgtt catacagcgc ctgcatccgc gcgcgttcgt cgccggtcag cgccaccgca
     2341 tcggtcgcgc cgacaaattc gcggagctgt tcggaattgt aaatattcgg cagcaccgag
     2401 gccacgcggc cgtcggcgag cagccattgg atcgcggcct ggccgagcgt gcggccatcc
     2461 cgctcgagaa aacgcagctg ctcgaccttg cgaacgccat tgatgagcca gctccgcggg
     2521 cggtggctgc ggtgatccgc cggctcaaac acggtgtctt ccgtgtattt gccttccagc
     2581 atgccggagg aatgcgggac gcggatgaag aattgcgtgc gcgccccggc ctcctcggcc
     2641 gcctgctgga tcgcccggcc ggggtgctgc tcgagaaaat tgtagatgtg ctgcaggatg
     2701 ccgggattgt gctcgcgaat ggcgcgcacg ccctcgccga gccagccgat cgccggcccg
     2761 agcgccgcgc cgccggtgcg gatctttccc tcggcgcgca agtcctccag cgtctcccac
     2821 acggcggcat cgtccacctg ctccatgcgg atgttgtgga gctgcagcac gtcgatgacg
     2881 tccgtccgca ggcgcgaaag cgcggcttcg gccgcccggc ggatggccgc ggggctgaaa
     2941 tcctggggaa tctcccgctg gccacggcgc tcattgccgt ggttcacaaa gtcgtagccc
     3001 accttcgtcg cgatcacgat gcgatcccgc tgcccggcaa acgcctgggc gatcaattcc
     3061 tcgctccggc cattgccata ggtgtcggcc gcgtcaaaaa gggtcacgcc caggtcggcg
     3121 gcttcccgca tgagctttac cgcttcctcg tcggtgtaat ttccccacca gcccgtggag
     3181 agcgtccaca gcccgaatcc cacctcgctg acttcgagag ccgtgccgcg cagtttgcga
     3241 tatttcatag gcgcagaata cttgactccc cc
//
LOCUS       DATDAY010000016         5453 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_299032, whole genome shotgun sequence.
ACCESSION   DATDAY010000016 DATDAY010000000
VERSION     DATDAY010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 5453)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5453)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5453
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_299032"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            61..699
                     /locus_tag="VIS74_00320"
     CDS             61..699
                     /locus_tag="VIS74_00320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5791708.1"
                     /translation="MNLRALIGPWLAAVTLLVGGALWLMTSAVPPKSGSPAAPATPKT
                     AADWRPGDRPPGGGAPPPPDPRIYQIALAQADGTQSLPEILEISHRLNEPEGNIHNDL
                     ETVQQLFSIYSRFNDNAVPAAPDNEGITSMLRGNNPKRFSVLPPGSSAKLLNAKGELI
                     DRWGTPYFMHTVSRELIEIRSAGPDRILWNGDDTQLPETSYYNASNAGPVGG"
     gene            1045..5268
                     /locus_tag="VIS74_00325"
     CDS             1045..5268
                     /locus_tag="VIS74_00325"
                     /inference="COORDINATES: protein motif:HMM:NF020390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 family protein"
                     /protein_id="HEY5791709.1"
                     /translation="MRTRLLTLSSTLLFGILAPSQAATVIIDNAAPSGVTVSGAWTAS
                     TFAPGFHGSNYLRDGNTGVNSSAALTVSGDIGTVTPAGSSSYDAATDRLTVSGSGKDI
                     WGNVDAFHFVRSGTKLTGDGTITARVESQQNTDNWAKAGVMIRETTAADSKQVMTAVT
                     PVNGVCFQRRPTTAGASINSSIGGLGAPRWVRLVRAGDTFTSFYSSDGTTWTQISWQN
                     IAMAADVEIGLCVTSHKNGTLSQVVFSNVTLTSGAASAASSVRLTPTLSTAGQQRVYL
                     RWPADASHASNASVAVVAAGGATPLAVNQRVDGGKWNPLGAYPFNEGTSGSVLIANTG
                     ANGEVAADAARWIPTLRYEAETIAFAESSAAVTAFTDPAASNGAGERLAATAAGDFVA
                     YTLPAVPAGTYQVKARYRAGGAAGLFQLAIDNADQGAVQDQYGATPKWIEADLGRVTF
                     GAAGDKVFKFTVTGKNAASTGYELTFDYLELQPFTDTDGDGMPDAWEIDYGLDPDSAA
                     DGGLDTDGDGQTNLQEYQAGTNPNPGNGNTGTVTLSADVANAYEKEGIPARVKITRTG
                     GSAAATIQFTRSGTAGAADYATKDANGGTLNGSILLPAGANSVDVVIQPAVDAVSEYP
                     EPATLTLTPGAGYSVGAANSATVTLNDATNVPANEKLYVAALTPAPGTNSSASGIATM
                     YVNGSNTAARVNLSFSGLTSGQTNSYIRYGITGGIGTELRPNMGIGQLVDVPWNIIPV
                     GTLSGQNLIDSLNEIGGKWAYTNIGTGNYPSGEIQGTWRKYEGSGTFTPPAGSPAIPP
                     LTGDDLARDVSRFLTQATFGPKKAEIDALVNSVNTTYAGDRIAAYNAWIDSQFALDQT
                     LLWEYNKAADRQDETLRGLGPYGYYAPSANCNHFFGWWTISVNAHDQLRQRYAAALSE
                     IIVISHASGLTVRPYGPDTFYDMLGASTSGNFRTLLEDTSKHPAMGEYLSSISNSKRT
                     VDAQGNTLTSPDENYAREILQLFSVGLLDLNPDGTLKLDAGGQAIQTYTQQDIAELAR
                     VFTGWGYSKRPGAAADGYPPIDNTTFYRLFSGGGPERHFQYEWIYPMKNYGPTYHDTG
                     AKTVLGSAIPAGLDGEADLDAALDIIFNHPNTGPFIARRLIQRLVTSNPSAGYIYRVA
                     QKFANNGSGVRGDMKAVIKAILLDYEARDLSVTNNVSYGKLKEPLMRMAQLFRSLDGG
                     SKLPISNLNPYSYPATQFDNFPASATHLRFGGLDTRIAQTPQSPPSVFNWFLPDYNPG
                     GDIGAAGLVAPEMQITSESTTFRAINYFSGFTYTSAAGNGGIPVYNDPDPKNDDVVLT
                     LTPLVSLYDSEISAGKTIPQALTTVLDYLDLLLLGGDFKPRYASAPAPNPRSRVIEEV
                     SAMSTATTLNRMENLLYLVMASSDFADQK"
BASE COUNT         1074 a         1966 c         1521 g          892 t
ORIGIN      
        1 cttcccgaac ctccaccgct tcgacaaccc cttcggctcc accgcgaacc tgctctacgt
       61 ttgaacctgc gcgctctcat cggaccttgg ctggcggcgg tcactctgct ggtcggcggc
      121 gctctctggc tgatgacctc cgccgtgccg cccaaaagcg gctcccccgc cgcaccggcc
      181 accccaaaga cggcggcgga ctggcggccg ggagaccgtc cgccgggagg cggggcgccg
      241 ccgccgcccg atccgcgcat ctaccagatc gcgttggcgc aagcggacgg aacccagtcc
      301 ctgccggaaa tcctcgagat cagccaccgg ctcaatgagc cggaagggaa tatccacaac
      361 gatctcgaga cggttcagca actcttctcg atttacagcc ggttcaacga caacgccgtc
      421 cccgcggctc ccgacaacga aggaatcacg tccatgcttc gcggcaacaa ccccaagcga
      481 ttctccgtgc tgcctcccgg cagctccgcc aagctcctga atgcgaaagg cgaactcatc
      541 gaccgctggg gaacccccta tttcatgcac accgtttccc gcgaactgat cgaaatccgt
      601 tccgctggcc ctgaccgcat cctttggaac ggggacgaca cccaacttcc cgagacctct
      661 tattataatg caagcaacgc cggccccgtg ggaggataat gcctgcacgg aaaaatccga
      721 ggaagtcgaa caagcgaaac gccttaaaaa ctgatacttt agctttacat tcttactagg
      781 atgtaaatgc ttcgagctag gtattgtccc cccaaacatg cctccccccc tcaaaaaaaa
      841 tcagctccgg tcccttggcg catttgcgcg gggtgtctgc cattgccctt ggcggcggat
      901 gggtggattt cttcggttcc gcctcctttg ggaaaataac ggtgactcgc cgatttcccc
      961 attccctttc ccggctcctg ccgctcatcg cggctcgccg attcctccac cctcccccag
     1021 ttaagcatcc ccctataaaa aaccatgaga acccggctcc tgaccctcag ttcaaccctt
     1081 ctttttggca tcctcgcacc gtcccaggcg gcgacggtga tcattgacaa cgccgctccc
     1141 agcggcgtga ccgtgtccgg agcctggacg gccagcacct tcgctccagg tttccacggt
     1201 tcgaattacc tgcgcgacgg aaacacgggc gtcaacagca gcgccgccct caccgtttcc
     1261 ggcgacatcg gcaccgtcac cccggcggga agttcgtcct acgacgcggc caccgaccgc
     1321 ctcacggtca gcggctcggg caaggacatc tggggaaacg tggacgcgtt tcatttcgtg
     1381 cgcagcggga cgaaactgac gggagacggc acaatcacgg cgcgtgtgga gagccagcag
     1441 aataccgaca attgggcaaa ggcgggcgtg atgatccgcg aaacgaccgc cgcggattcc
     1501 aagcaagtca tgaccgcggt cacccccgtg aacggcgtgt gtttccagcg gcgccccacg
     1561 accgcgggag ccagcatcaa cagctcgatc ggcggcctgg gcgctccccg ctgggttcgc
     1621 ctcgtccgcg cgggagacac gttcacgagc ttctactcca gcgatggcac cacctggacc
     1681 cagatctcct ggcaaaacat cgccatggcg gcggatgtcg agatcggcct ctgcgtgact
     1741 tcgcacaaga atggaacgct tagccaggtg gtcttcagca atgtaaccct caccagtggc
     1801 gctgcttcgg ccgcttcctc ggtccgcctg acccccacgc tttctaccgc gggccagcag
     1861 cgggtctatc tgcgctggcc ggcggacgcc tcccatgcct cgaatgcctc cgtggccgtg
     1921 gttgcggccg gcggcgccac ccctctggcc gtcaaccagc gcgtggatgg cggcaaatgg
     1981 aatccactcg gcgcttaccc attcaacgag ggaacctccg gcagcgtcct gatcgccaat
     2041 acgggtgcca atggcgaggt cgccgccgac gcggcgcgct ggatacccac cctgcgttat
     2101 gaggccgaaa ccatcgcctt cgcggaatcc tccgcggcgg tcacggcgtt caccgacccg
     2161 gcagccagca atggcgccgg cgagcggctg gccgccacgg cggcgggcga ttttgtcgcc
     2221 tacacccttc cggcggttcc cgccggcacg tatcaggtca aggcccgcta ccgggcgggc
     2281 ggcgcggcgg gcctgttcca gctggcgatc gataacgccg atcagggcgc cgttcaggat
     2341 caatacgggg cgacgcccaa gtggatcgag gccgatctcg gccgcgtcac cttcggcgcc
     2401 gccggcgaca aggtcttcaa gttcaccgtg acgggcaaaa acgccgccag cacgggctac
     2461 gagctgacgt tcgattatct cgagctgcaa ccctttaccg acaccgacgg agacggaatg
     2521 cccgatgcct gggaaatcga ctacggcctc gatcccgaca gcgccgccga cggcggcctg
     2581 gacaccgacg gcgacggcca gaccaacttg caggaatatc aagcaggcac caacccgaat
     2641 cccggcaatg gcaataccgg aacggtgacc ctctcggcgg acgtggcgaa tgcctacgag
     2701 aaggaaggca tccccgcccg ggtgaagatc acccgcaccg gcggctcggc ggcggccacc
     2761 atccagttca cgcgttcggg aacggcgggc gccgccgatt acgccacgaa ggacgccaac
     2821 ggcggcacgc tcaacggatc cattctcctg cccgccgggg ccaactccgt ggacgtggtg
     2881 atccagccgg ccgtggacgc cgtgagcgaa tatccggaac cggccaccct gacgctcact
     2941 ccgggagcgg gctacagcgt cggcgcggcc aacagcgcga cggtgaccct caacgacgcc
     3001 acgaatgttc cggccaatga aaaactctat gtcgcggcgc tgaccccggc tccgggaacg
     3061 aacagctccg cctcgggaat cgcgacgatg tatgtcaacg gttcgaacac cgccgcccgc
     3121 gtcaacctga gcttcagcgg cctgacttcc ggacagacga actcctacat tcgttacggc
     3181 atcaccggag gcatcggcac ggagctgcgc cccaacatgg gcatcggaca gctggtggac
     3241 gtgccctgga acatcattcc cgtgggcacc ctctccgggc aaaacctcat cgacagcctg
     3301 aatgaaatcg gcggcaagtg ggcctacacg aacatcggca ccggcaacta cccctccggg
     3361 gaaatccagg gcacctggag gaaatacgag ggctccggca ccttcactcc gccggccggc
     3421 tcgcccgcga ttcccccact gaccggcgac gatctcgccc gcgacgtctc ccgcttcctc
     3481 acccaggcca ccttcggtcc aaaaaaggcg gaaatcgacg cgttggtcaa cagcgtcaac
     3541 accacctacg ccggcgaccg catcgccgcc tataatgcct ggatcgacag ccagttcgcc
     3601 ttggaccaga ccttgctctg ggaatacaac aaggccgccg accggcagga cgagacgctg
     3661 cgcggcctcg ggccctacgg ttattacgcg ccctcggcca actgcaatca tttcttcggc
     3721 tggtggacga tctccgtgaa cgcccacgac cagctccggc agcgctacgc cgccgcgctg
     3781 agcgaaatca tcgtcatttc gcacgcttcc ggcctcaccg tccgccccta cggcccggac
     3841 accttctacg acatgctggg cgcgagcacc tcgggaaatt tccgcacgct gcttgaggac
     3901 accagcaaac atcccgcgat gggcgaatac ctcagcagca tctcgaactc gaagcgcacc
     3961 gtggacgccc aggggaacac gctcacgagc cccgacgaaa actacgcccg cgagatcctc
     4021 cagctcttct cggtcggcct gctggacttg aaccccgacg gcaccctgaa gctggatgcc
     4081 ggcgggcagg cgatccagac ctacacgcag caggacatcg ccgagctggc ccgcgtcttc
     4141 accggctggg gctacagcaa gcggcccggc gccgccgcgg acggctaccc tcccattgac
     4201 aacaccacct tctaccggct gttttccggc ggcggtccgg agcgccattt ccaatacgaa
     4261 tggatctacc cgatgaagaa ctacggcccg acctaccatg acacgggcgc aaagacagtc
     4321 cttggttccg ccattcccgc cggattggat ggcgaggccg acctggatgc cgccctggat
     4381 atcattttta atcatcccaa caccggcccc ttcatcgccc ggcgtttgat ccagcggctg
     4441 gtgacgtcga accccagcgc gggctacatt taccgcgtcg cgcagaagtt cgccaacaac
     4501 ggcagcggcg tgcgtggcga catgaaggcg gtcatcaagg cgatcctcct cgactacgag
     4561 gcccgcgacc tgtcggtcac caacaatgtc agctatggca agctcaagga accgctcatg
     4621 cggatggcgc agctcttccg ctcgctggac ggcggctcga agctccccat tagcaacctg
     4681 aatccataca gctatcccgc cacccagttt gacaacttcc cggcctccgc cacccacctc
     4741 cggttcggcg ggctcgacac ccgcatcgcc cagacgcccc aaagcccgcc ctccgtcttc
     4801 aactggttcc tgcccgatta caatcccggc ggggacatcg gcgcggccgg cctcgtcgcg
     4861 ccggagatgc agatcaccag cgagagcacc acattccgcg cgatcaacta cttctctggg
     4921 ttcacctata cctcggcggc cggcaatggc ggcattcccg tctataacga tcccgacccg
     4981 aaaaacgacg acgtggtcct gaccctgacg cctctggtct cgctctatga cagcgaaatc
     5041 agcgccggca aaacgatccc ccaggccttg accacggtat tggactacct ggatctgctg
     5101 ctcctgggcg gcgacttcaa gccaagatac gccagcgcgc ccgccccgaa tccccgcagc
     5161 cgggtcatcg aggaggtcag cgcgatgagc acggccacca ccctgaaccg catggagaac
     5221 ctgctctacc tcgtgatggc ctcctccgat ttcgccgatc aaaaataatc ccccatccgt
     5281 catcccatga aaagccaaga caaggaactg caattctccc gccggagttt catccggcaa
     5341 tccgcctgcg ccgcgctcgg ttatgccggc gtggtcaacg cgctcaccca gctcagcatc
     5401 atgaactccg cgctcgggca atcctccctg gacggctaca aggcgctggt cgt
//
LOCUS       DATDAY010000017         4998 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_291389, whole genome shotgun sequence.
ACCESSION   DATDAY010000017 DATDAY010000000
VERSION     DATDAY010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 4998)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4998)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4998
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_291389"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..410)
                     /locus_tag="VIS74_00330"
     CDS             complement(<1..410)
                     /locus_tag="VIS74_00330"
                     /inference="COORDINATES: protein motif:HMM:NF013315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HEY5791710.1"
                     /translation="MALLSLMTAAGLTFGGLAEWENLRARMVEEQLAGRDITDRRVLA
                     MMNRVPRHEFVPAAWQASAYDDMPLPIGHGQTISQPYIVAFMPQLLDPQPDDRILEVG
                     TGSGYQAAVLAGLVKEIDSIEIVEPLAEQAKKTLA"
     gene            913..2700
                     /locus_tag="VIS74_00335"
     CDS             913..2700
                     /locus_tag="VIS74_00335"
                     /inference="COORDINATES: protein motif:HMM:NF020390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 family protein"
                     /protein_id="HEY5791711.1"
                     /translation="MRPAFLQLALILALGLRLAIPAHGKEAPPAFASNPPASITATAA
                     PSDRRLTGEQLASRFLNQATFGATPEEITALSASLAAKGDAAFSDWIDAQFQKPVSEK
                     DLALPTFRAAFPPDYPKSGYREHTHEGGALRASLMIRDNDHPLRRRVAYALSQIFVVC
                     EEGGLFGSPEGMCDWSDLLFQNAFTDFLTLLKAVTYHPVMASYLSLNGNAKAGYYNPG
                     SRPDENYAREVMQLFTIGLVRLNPDGSYVTDAAGRTIPTYDQTTITEMARVFTGLNYP
                     AGTVEYTTLRDGKRQRLTREGNVRFGACVTKEKKHDTEAKSFLGASLPAGQDTKKDID
                     DTLQILASHPNTAPFISKALIQRLTTSNPSPAYVKRVAETFAAGQGEMKAVVRAILLD
                     EEARDPRNVNNDQFGKLREPWLRVTQLARAFRAQPAEKQPWFPVYARDLLPKLGQFPF
                     WAPSVFNFYLPSFQPAGAVAERNARIPDNAVPIVAPEFQIMNSNTALLLPNYLMDCLE
                     AQKVSNKPKRPIVLDLNPQANLAQNAGELVANVDILLTSGTMSPGTRQVITEAVEALT
                     PAADPKVRRERAKMAIYLTLVSPDYAIQR"
     gene            2714..4192
                     /locus_tag="VIS74_00340"
     CDS             2714..4192
                     /locus_tag="VIS74_00340"
                     /inference="COORDINATES: protein motif:HMM:NF019040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEY5791712.1"
                     /translation="MKEPNGLTRRQFLVRAGCASLGSTGVLSALLNLGMAAKMAASDL
                     NDYKAMVCVFLLGGNDSYNVLAPANRDEYRYYLDSRGGLYDVENNPAGLALAAPGEMP
                     GSLLPIQPVNLPGRGFGIHPAMPDIKRLFDAKALSFVANVGSLQKPLPNVAAYRNASG
                     DRPLGLFSHSDQQQQWQTCMPDRRSGIGWAGRAMDILQNDQSAGQKSTFNISLSGTNI
                     LQTGSQVVPYSMSLDGLVPLKGYALSEPSANASFNEKETYARSAAVKKMMDGMYRNLF
                     ETTYARITLDAQDALTQYQKKLDTDAYPQWDTANQPVLEQQLKLVANVISAYRNSPTK
                     RQTFFISISGFDLHHGLMLEHPDVLKALNSALGNFWNHLSADLKPAVTLFTASDFGRT
                     LSGNGSGADHGWGGNQIVMGGAVQGGRIFGTYPTFAPSVIETTYDVGQGRLIPGFSVE
                     EYVYPILRWFGVSDSDLFGAVFPGYTSRFGSGGIRASYPLYG"
     gene            complement(4202..4732)
                     /locus_tag="VIS74_00345"
     CDS             complement(4202..4732)
                     /locus_tag="VIS74_00345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5791713.1"
                     /translation="MKRALAILLALSSAVLADGGAVLFQKQEGNLQVTVFGLPSPLRA
                     GPVDISVLVQNAETRAPVLDAQVSVSVTPLSADQLADGEVWTPPCCSMKRSDDFYAAT
                     REQAQNKLMYHTFFPIPASGQWNLAVEIRHQNGARVFVETVDVAPPQKPALAYWPLLV
                     LPIAAVGGFAINRALR"
     gene            complement(4735..>4998)
                     /locus_tag="VIS74_00350"
     CDS             complement(4735..>4998)
                     /locus_tag="VIS74_00350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5791714.1"
                     /translation="HDAGLSRQMPDWVIGSFAFMDLPGLELLLLDLGFLLTLYFLWKR
                     ARNLVVKRALGVFLPWGILALLLYAAGVWIIFQPMEMRGMLVH"
BASE COUNT         1028 a         1683 c         1418 g          869 t
ORIGIN      
        1 gccagcgttt ttttggcctg ttcggccagc ggctcgacga tttcgatgga atcgatctcc
       61 ttcaccagcc cggcgaggac ggcggcctgg tagcccgagc ccgtgccgac ttccaggatg
      121 cggtcgtccg gttgcgggtc gagaagctgg ggcatgaacg ccacgatgta gggttgcgaa
      181 atcgtctggc catgcccgat gggcagcggc atatcgtcgt aagcggaggc ctgccaggcg
      241 gccggcacaa attcgtgccg gggcacgcgg ttcatcatgg cgagcacgcg gcggtccgta
      301 atgtcgcggc cggcgagttg ctcctccacc attctcgcgc gcaaattttc ccattcagcg
      361 agaccgccga atgtcagccc cgccgcggtc atcagagaga gaagagccac ttctcaacct
      421 ccgcgctggc tggggaaaat gcaaccgccg tgaatggact cagattttta aaaatcctca
      481 atcctcgccg caaaaaatct gcgaaaatcg gtgaaatcgg cggatatttt cccttcatcc
      541 cattgaatca gcggtttcta aaggcaactg cacccccgga accgcgccag cgcgggtcaa
      601 agctcgaaac gcccggttcg agcggttttt caaaagggag ttccgaaaaa gtgatccatt
      661 gcaggggcgg ttgtgccaag cgcccccgcg taaacgggga tgcggaatcc ccgtttcccc
      721 gcaaacaaag gcggttggca caagcgcctc aacgcgtttt ggtatttcgg aaccctcttt
      781 tgaaaaacat gatacgcgaa gcgggcggga ttcccatttg gcaaatgccg ttttcgcggt
      841 agtttttccg gaaccgatct cctcctggcg ggttcaattc cggagatcga cagcgagaaa
      901 ccttttgttt gcatgcgacc ggcttttctt cagttagccc ttattctggc cctcggcctg
      961 cggttggcca tcccagccca cggcaaggaa gcgccgccag cttttgcctc caatccgccg
     1021 gcttcaatca ccgccacggc ggccccgtcc gaccggcggc tcaccggcga gcagctcgcg
     1081 tcgcgtttcc tcaaccaggc caccttcggc gccactcccg aggaaatcac cgccctctcc
     1141 gcgagccttg ccgccaaagg ggacgccgcc ttctcggatt ggatcgacgc gcaattccaa
     1201 aagccggtga gcgaaaagga tctcgcgctg cccaccttcc gcgccgcctt tccgccggat
     1261 tatcccaaaa gcggctaccg cgagcacacc cacgaagggg gggcgctccg cgccagcctc
     1321 atgatccgcg ataacgacca cccgctccgg cgccgggtgg cgtatgccct gagccagatt
     1381 tttgtcgtct gcgaggaggg cggcctgttc ggctcccccg agggaatgtg cgattggtcc
     1441 gacctgctct tccagaacgc gttcactgat tttctgacgc tcctgaaagc cgtcacctac
     1501 catccggtca tggccagtta cctgagcctc aacgggaatg ccaaggcggg ctactacaat
     1561 cccggctcgc ggccggatga aaattacgcc cgggaggtca tgcagctttt caccatcggg
     1621 ctcgtccggc tcaacccgga cggaagttac gtgaccgacg ccgcgggccg cacgattccc
     1681 acctacgacc aaacgacgat caccgaaatg gcgcgcgtct tcacggggct caactacccc
     1741 gccggaacgg tcgaatacac gaccttgcgc gacggcaagc gccagcgcct cacccgggaa
     1801 ggcaacgtgc ggttcggcgc ctgcgtcacg aaggagaaaa agcatgacac ggaggcgaag
     1861 tcttttttgg gcgccagcct tcccgccggg caggacacaa aaaaggacat cgacgacacc
     1921 ctccagattc tggcgagcca tcccaatacc gcccccttca tttccaaggc gctcatccag
     1981 cgtctgacga cttccaatcc aagcccggcc tacgtcaagc gggtcgccga aacctttgcg
     2041 gccggccaag gcgaaatgaa ggccgtcgtc cgcgcgatcc tgctcgatga agaggcgcgg
     2101 gatccccgca acgtgaacaa tgaccaattc ggcaagctcc gggagccctg gctgcgggtg
     2161 acccagctgg cgcgggcgtt ccgcgcgcag ccggcggaga agcagccttg gttccccgtc
     2221 tatgcgcgcg acctgctgcc gaagctgggc cagtttccct tctgggctcc cagcgttttc
     2281 aacttctacc tgcccagctt ccagccggcc ggagccgtcg ccgaacgaaa cgcccgcatt
     2341 ccggacaacg ccgtcccgat cgtcgccccg gaatttcaaa tcatgaactc gaataccgcg
     2401 ctcctcctgc ccaattacct gatggattgc ctggaggctc aaaaggtcag caataaaccc
     2461 aagcggccaa tcgtgctgga tctcaacccc caggcgaacc tggcgcaaaa cgcgggggaa
     2521 ttagtcgcca atgtggacat cctcttaacc agcggcacga tgagcccggg cacgcggcag
     2581 gtcatcaccg aagcggtcga agcgttgacc cccgccgccg acccgaaggt gcgccgcgaa
     2641 cgggccaaaa tggccatcta cctcaccctg gtttccccgg attacgccat ccaaagataa
     2701 cgacccaccc accatgaaag aaccaaacgg attaacccgc cgccaattcc tggtccgagc
     2761 cggctgcgcc agcctgggaa gcaccggcgt cctgtccgcg ctcctgaatc tgggaatggc
     2821 ggcaaaaatg gccgcatccg accttaacga ttacaaggcg atggtctgcg tgttcctgct
     2881 gggcggcaac gattcctaca acgtgctggc gcccgccaac cgcgacgaat accgctatta
     2941 cctggactcc cgcggcgggc tttacgatgt cgaaaacaat ccggccgggc tggccctcgc
     3001 cgcgccggga gagatgccgg gctcgcttct gccgatccag ccggtcaatc tcccgggccg
     3061 cggcttcggc attcatcccg ccatgcccga catcaagcgc ctcttcgacg cgaaggcgct
     3121 ttccttcgtc gccaatgtag ggtcgctgca aaagccgctc cccaatgtcg ccgcctatcg
     3181 gaacgcctcg ggagatcgcc cgctggggtt gttttcccat tccgaccagc agcagcaatg
     3241 gcaaacctgc atgcccgacc ggcgctccgg catcggctgg gcggggcggg ccatggacat
     3301 cctccaaaac gaccaaagcg ccggccagaa aagcaccttc aatatttccc tctcgggcac
     3361 caacatcctg cagacgggga gccaggtggt cccctactcc atgagcctgg acggcctcgt
     3421 gccgctgaag ggttacgcgc tttccgagcc ttcggccaac gcctcgttca acgaaaagga
     3481 aacctatgcg cgcagcgcgg cggtcaaaaa aatgatggac gggatgtatc gcaacctctt
     3541 cgagacgacc tatgcccgga tcacgctcga cgcgcaggat gcgctgaccc aataccagaa
     3601 aaagctggat accgacgctt acccacaatg ggatacggcc aatcaacccg tgctggagca
     3661 acagcttaaa ctcgtcgcca acgtcatctc ggcctaccgg aacagcccga ccaaacggca
     3721 gacctttttc atctcgatca gcggattcga cctgcaccac ggcctgatgc tggagcaccc
     3781 ggacgttctc aaggcattga actccgcgct gggcaatttc tggaaccacc tgtccgcgga
     3841 tctcaagccg gcggtcaccc ttttcaccgc ctccgacttt gggcggaccc tgagcggcaa
     3901 cggaagcggc gccgaccatg gatggggcgg aaaccagatc gtcatgggcg gagccgttca
     3961 gggcggccgg atttttggca cgtatcccac cttcgccccg agcgtcatcg agaccactta
     4021 cgacgtcggc cagggccgcc tgatccccgg cttctccgtc gaggaatacg tgtatcccat
     4081 cctgagatgg ttcggggtgt ccgacagcga tcttttcggc gccgtcttcc cgggttacac
     4141 gagccgtttc ggcagcggcg gaatccgagc cagctacccg ctttacggct gagcgccgga
     4201 atcagcgcag ggcgcggttg atcgcgaaac cgcccacggc ggcaatcggc aaaaccagca
     4261 gcggccagta agccagcgcc ggtttttgcg gcggcgcgac gtccaccgtt tcgacaaaaa
     4321 cgcgggcgcc gttttgatgg cggatctcca ccgcgaggtt ccactgcccc gacgcaggaa
     4381 tcggaaaaaa ggtgtgatac atcagcttgt tctgcgcctg ctcgcgggtg gccgcgtaga
     4441 aatcgtccga gcgtttcatg ctacagcagg gcggcgtcca gacttccccg tccgccagct
     4501 gatccgccga aagcggggtg acggaaacgc tcacctgcgc atccagcacg ggagcccggg
     4561 tttccgcatt ctggaccagc acgctgatgt ccaccggccc ggcgcgcaac ggcgagggca
     4621 acccgaacac ggtcacctga agatttccct cctgtttttg aaacagcacc gcgccgccat
     4681 ccgccaggac ggccgaactc agggccagga gaatcgccag cgcgcgcttc atggtcagtg
     4741 aaccagcatg cctctcattt ccatgggctg aaagataatc caaacccccg ccgcgtaaag
     4801 cagcagcgcg agaatccccc acggcagaaa gacgcccaag gcgcgcttga ccaccagatt
     4861 gcgggcgcgc ttccacagaa aatacagcgt cagcagaaat ccgaggtcga gcagcaggag
     4921 ctccagcccc ggcaaatcca tgaaggcgaa cgacccgatc acccaatccg gcatttgccg
     4981 gcttaaaccc gcgtcgtg
//
LOCUS       DATDAY010000018         4309 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_286336, whole genome shotgun sequence.
ACCESSION   DATDAY010000018 DATDAY010000000
VERSION     DATDAY010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 4309)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4309)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4309
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_286336"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..333)
                     /locus_tag="VIS74_00355"
     CDS             complement(<1..333)
                     /locus_tag="VIS74_00355"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY5791715.1"
                     /translation="MKEVYEFDLVCIGSGPAGQRAAVQAAKLGKKVAVIEKQTCIGGV
                     CIETGTIPSKTFREAVRRLYAQTSFGQGAEAPRRQRPTMPQLLAQVDRVVQKESEGVR
                     DALNRNDVT"
     gene            complement(680..1948)
                     /gene="bioA"
                     /locus_tag="VIS74_00360"
     CDS             complement(680..1948)
                     /gene="bioA"
                     /locus_tag="VIS74_00360"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: protein motif:HMM:TIGR00508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HEY5791716.1"
                     /translation="MSNPSELDQRYSWHPFTQMRDWCAREPLMLVEGDGAILRDSAGN
                     EYLDGNSSIWTNLHGHRHPKINAAIRAQLDRVAHVSYLGSANEPASILAEKLVKLFPP
                     GTLERVFYSDDGSTAIEVALKMAAQFWQLGGQPRRSIFVSFDQAYHGDTAGAASLGGI
                     SVFHDRFAKFHFPCRRVKTVAELRELPELRNGEAAAVVIEPLIQGAAGLRLWPKGMLA
                     ELRQLCDEADTFLILDEVMTGFGRTGTMFACEQEGVIPDFIALAKGLTGGYLPLAATL
                     TTRRVYEQFLGEYEELKTFFYGHSYTANPLGCAAALANLQIFEEEKTLDQLPPKIQLM
                     AQRLAALEWTTRQCGFIAGIDLVKPSGEAFDWREQTGARVCLAARRHGLLTRPIRDTI
                     VLMPPYCVTADQLHGAFDALEAAIAETLGA"
     gene            2047..2976
                     /locus_tag="VIS74_00365"
     CDS             2047..2976
                     /locus_tag="VIS74_00365"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HEY5791717.1"
                     /translation="MSPLLKIALYLLAVLAAAVFLSPPVYGFFQWLADVGIFTGFAGF
                     PFHRFFTRIAQIAALVLLWPLVRWLNIRSIRELGIDQNPVFGRDLLAGFAITLVLMAI
                     LGGIYFGAEIYRVRPHVDWIKLLRIAASAAFVGVIEEFLFRAVLLGLAVKAFGRTGGV
                     LLSAGLFAAVHFLRPSREGAEVVTWTSGFGQLFSFVEAAPALPLLVAGMASLFAVGLV
                     LASVTVRTHSLALAIGIHAAWVFGQQSLSVIGKYRVKPGDALLPWVGPNLVSGAVPTG
                     LLPLAVLGVTALLLGLYLKYVPRPPATLAEGRD"
     gene            2933..3658
                     /locus_tag="VIS74_00370"
     CDS             2933..3658
                     /locus_tag="VIS74_00370"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HEY5791718.1"
                     /translation="MSRVRPQPWLKAAIEVALPAHCVSCGNAARRGAAFCRDCRRRLP
                     RIRGARCEVCSEPFSAADEGLVCTNCRGRNFYFVATIASYRANGEIRELIHRLKYNRE
                     LHLRRPLGWLLRRNLLDDRLRGVKFDGLVPVPLHPLREREREFNQSRLLAEELAQRAK
                     VPVWDDLKRIRATETQTHFDRHERMQNLRGAFVLRRMDRVPGQTLALIDDVFTTGSTL
                     NECARVLLEAGARTVWALTVARA"
     gene            3671..>4309
                     /locus_tag="VIS74_00375"
     CDS             3671..>4309
                     /locus_tag="VIS74_00375"
                     /EC_number="2.1.3.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HEY5791719.1"
                     /translation="MPIFKAPVLSVFSRKRREMPEGLWTKCPSCGELIHNIALEQNLS
                     VCPKCDHHFTLGAQKRIELIVDEGSFEERDANLVSVDPLKFKGVATYEERLKTYRKKT
                     GLKDAVITGTARINSHPVGLAVMDFAFLAATMGSVVGEKITRIIELSTERNHPVLIVS
                     ASGGARMYESMLSLMQMAKTCGALARHAGAELPYISILTNPTTAGVMASYASV"
BASE COUNT          713 a         1377 c         1402 g          817 t
ORIGIN      
        1 ggtgacatcg ttgcggttca gggcatcccg cacgccctcg gattcctttt ggacgacccg
       61 gtccacctgg gccagcaatt gcggcatggt cggacgctgg cggcggggcg cctcggctcc
      121 ctggccgaag ctggtctggg cataaagccg ccgcaccgct tcgcgaaagg tcttggaggg
      181 aatcgtgccg gtttcgatgc aaacgccgcc gatgcacgtc tgcttttcga tgacggcgac
      241 cttcttcccc aacttggccg cttgcaccgc cgcgcgctgg ccggcgggac cgctgccgat
      301 gcagacgaga tcgaattcgt aaacttcttt cataaggtcg aatagggtgg atgggttggc
      361 cgcaaaaccc tccaggcagc acatcccgcg ccaaggttgg caacgaattc gacaatctcc
      421 cgcgcggagg ccgccgagcg cgggagagaa aaatcacggc ggtttcgtcg aaaccgccct
      481 gccttccgag ggtagggacg gctcgatgag ccgtccgtcg cctctcccca cgcgcccgat
      541 tcgatcgaaa actcgcgcgt ttttctttta tgttgtcgcc gtgaccggcg gtgccggcga
      601 caccgcccta ccgccagagg ggtcttccgg tggggcggcg gtgccaacgc cgccgagagg
      661 aacgcctcca attaaaccgt caggcgccca gcgtttcggc gattgccgcc tccagcgcgt
      721 cgaacgcgcc gtggagctgg tccgcggtca cgcaatacgg cggcatcagc acgatcgtgt
      781 cgcggatcgg ccgggtgagc aggccgtgcc ggcgggcggc gaggcagacc cgcgcgccgg
      841 tttgctcccg ccagtcgaag gcctcgccgg agggcttcac gagatcgatg cccgcgatga
      901 agccgcattg ccgggtcgtc cattccaggg cggcgagccg ttgcgccatt aactgaattt
      961 tcggcgggag ctgatcgagc gttttttcct cctcgaagat ttggagattc gccagcgcgg
     1021 cggcgcagcc gagcgggttg gcggtgtaac tgtggccgta gaagaacgtc ttcagctcct
     1081 cgtattcgcc caggaattgc tcgtaaaccc ggcgggtggt caaggtggcg gccaatggca
     1141 gatacccgcc cgtgagtccc ttcgccaggg cgataaaatc cgggatgacg ccttcctgct
     1201 cgcaggcaaa catcgtgccc gtccggccga agccggtcat gacctcgtcg agaatcagga
     1261 aggtgtccgc ctcgtcgcag agctggcgga gttcggcgag cattcccttt ggccagagcc
     1321 gcaggcccgc cgcgccctgg atgagcggct cgatgaccac ggcggcggcc tcgccattgc
     1381 gcagctcggg cagctcccgc agttcggcga ccgttttcac gcgccggcag ggaaaatgga
     1441 atttcgcaaa gcggtcgtga aacaccgaga tgccgccgag gctcgcggcg ccggcggtat
     1501 cgccgtggta ggcctggtcg aacgagacaa aaatggaccg ccgcggctgg ccgccgagct
     1561 gccagaattg cgcggccatc ttcagcgcga cttcgatggc cgtcgagccg tcgtcggaat
     1621 aaaacacgcg ctccagcgtt ccgggcggaa acagcttcac cagcttctcc gcgaggatcg
     1681 aggcgggttc gttggccgag ccgaggtagg acacgtgcgc cacgcggtcg agctgggccc
     1741 ggatggcggc attgattttc ggatggcgat ggccgtgcag gttcgtccaa atggaggagt
     1801 tgccgtcgag gtattcgttc ccggccgaat cgcgcaggat ggcgccatct ccctccacca
     1861 gcatgagcgg ctcccgcgcg caccagtccc gcatttgggt gaagggatgc caggagtagc
     1921 gctgatcgag ttcggacgga ttcgacatga cttcggaata ataaccatga agggcatgaa
     1981 gaccatgaag ggctcaagat tttcttcatg ttcttcatgt tctccatggt agaagattcg
     2041 gactccatgt ccccgttgct gaaaatcgcc ctctacctcc ttgccgtttt ggcggcggcg
     2101 gtttttctgt ccccgccggt ttacggtttt tttcaatggc tcgcggacgt cggcatcttc
     2161 acggggtttg ccgggttccc cttccatcgc tttttcacgc ggatcgccca gatcgccgcg
     2221 ctggtgctgc tctggccgct ggtgcgctgg ttgaatatcc gcagcattcg ggaactgggc
     2281 attgatcaga atccggtttt cggccgggat ctcctggccg gttttgccat cacgctcgtc
     2341 ctcatggcga tcctcggcgg catttatttc ggcgccgaaa tttaccgcgt tcgcccgcac
     2401 gtggactgga taaagctgct gcgcatcgcc gcctccgcgg cgttcgtggg ggtcatcgag
     2461 gagtttttgt tccgcgccgt gctgctgggc ctcgcggtga aggcgtttgg gcggacgggt
     2521 ggagtcctct taagcgcggg cctcttcgcg gcggtgcact ttctgcggcc atcccgcgaa
     2581 ggcgccgagg tggtgacctg gacgagcgga ttcggccagc tgttcagttt tgtggaggcc
     2641 gcgcccgcgc tgccgttgct ggtggcggga atggcgtcgc tcttcgcggt cgggctggtg
     2701 ctggcgtcgg tgacggtccg gacccactcc ctggcgctgg cgatcggcat ccacgccgcg
     2761 tgggtgttcg gccagcaaag cctgagcgtc atcggcaaat accgcgtgaa gccgggggat
     2821 gcgctgctgc cgtgggtggg gccgaacctc gtgagcggcg cggtgccgac cggcctgctg
     2881 ccgctggcgg tcctgggcgt caccgcgctg ctgctgggcc tttatttgaa atatgtcccg
     2941 cgtccgcccg caaccctggc tgaaggccgc gattgaggtc gcgctgcccg cgcattgcgt
     3001 gagctgcgga aacgcggccc ggcggggcgc ggcgttttgc cgggactgcc gccggcggct
     3061 gccgcgcatc cgcggcgcgc gctgcgaggt gtgctccgag ccgttcagcg cggcggatga
     3121 agggctggtc tgcacgaact gccgcggccg gaatttttac ttcgtggcga cgatcgcgag
     3181 ctaccgggcg aatggggaaa tccgcgaatt aatccatcgc ctgaaataca accgcgagct
     3241 ccacttgcgg cgtccgctgg gctggctgct gcggcgcaac ctgctcgacg accggctgcg
     3301 cggcgtgaaa ttcgacggcc tcgtgcccgt gcccctgcac ccgctgcggg agcgcgagcg
     3361 ggagttcaac cagtcccgcc tgctggccga ggagctggcg cagcgagcca aagtgccggt
     3421 ctgggatgac cttaaacgaa tccgggccac ggaaacccag acgcattttg atcgccacga
     3481 acgcatgcaa aacttgcgcg gcgcctttgt cttgcgcaga atggatcgcg tgccggggca
     3541 gaccctggcg ctcattgatg atgtttttac caccggatca accctcaacg aatgtgcccg
     3601 cgtgctcctc gaagcaggcg cccgcacggt ttgggcgctg accgtggcgc gagcctaacc
     3661 gtacaccgat atgccgatct tcaaagcacc cgtcctcagt gtcttttccc gcaaacgccg
     3721 cgagatgccc gaaggcctct ggaccaaatg cccctcctgc ggcgagctga tccacaacat
     3781 cgccctagag cagaatctct ccgtctgccc gaagtgcgac caccatttca cccttggcgc
     3841 ccagaagcgc atcgaactca tcgtggacga aggcagcttc gaggagcgcg acgcgaatct
     3901 cgtttccgtg gacccgctca agttcaaggg cgtggccacc tacgaggagc gcctcaagac
     3961 ctaccgcaaa aaaaccggcc tcaaggatgc ggtcatcacc ggcacggccc gcatcaacag
     4021 ccatcccgtc ggcctggccg tcatggactt cgcctttctc gcggcgacca tgggatcggt
     4081 cgtcggcgaa aagatcacgc gcatcatcga actgagcacc gagcggaatc acccggtcct
     4141 cattgtcagc gcctccggcg gcgcgcgcat gtatgaaagc atgctgagcc tcatgcaaat
     4201 ggcgaagacc tgcggcgcgc tcgcgcggca tgccggggcg gagctgccct atatttccat
     4261 cctgaccaat cccaccacgg ccggcgtcat ggccagttat gcctcggtg
//
LOCUS       DATDAY010000019         2420 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_282633, whole genome shotgun sequence.
ACCESSION   DATDAY010000019 DATDAY010000000
VERSION     DATDAY010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2420)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2420)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2420
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_282633"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..769)
                     /locus_tag="VIS74_00380"
     CDS             complement(<1..769)
                     /locus_tag="VIS74_00380"
                     /inference="COORDINATES: protein motif:HMM:NF018942.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030416 - methylamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MauE/DoxX family redox-associated membrane
                     protein"
                     /protein_id="HEY5791720.1"
                     /translation="MKAFLFKHPWLAGGLALIFLLAGASKLGHSERTFAGIQQYGLFA
                     ANPAYAIAEALPWIEIGLGVSLLVPRLRFAALGGAGLLQLLFFIGLVQAVLRGREIVC
                     VCFGPALGGAVELFLQETLLLGLTLLAGWREWKFRTNELESLLAFRQHRGVKFPGVAF
                     VLGFLLANSLQAGLVDSLDSKELRTLERGEPVLVTEEVKGFPWPRIAVYRMVDSTPEE
                     AMAVFFDYNSAREFIPNVKKSEISKDLNACSQEVAYAV"
     gene            complement(943..1575)
                     /locus_tag="VIS74_00385"
     CDS             complement(943..1575)
                     /locus_tag="VIS74_00385"
                     /inference="COORDINATES: protein motif:HMM:NF028235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEY5791721.1"
                     /translation="MSEVVLNSRRPEETRRKLLDASRDLILTQGFTGTRVDQICRMAG
                     VTKGAFFHHFQSKDELGQAALADWAHFGMDLYAAAKSQPARHPLDHLHRFFDIMIGFV
                     RETPGPVTCVVGIISQEMSLANPVLQQACATYLGDWTAFTRQLLDEAKAAQPPKTDFD
                     SEELAWFLNGLWQGSMLVAKARQDPRIIVLNLERARAYVDDLFGIPAAPG"
     gene            complement(1774..>2420)
                     /locus_tag="VIS74_00390"
     CDS             complement(1774..>2420)
                     /locus_tag="VIS74_00390"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEY5791722.1"
                     /translation="LETVLAEIEAGAKSVVVVGHRPHAGCAGEVLGILTVGDAIRPNA
                     AEAIRRLHASGIRKVVMLSGDNARTANAIAREAGIDEAHGDLLPENKIEQVRKLAADY
                     GGAGMVGDGVNDAPALAAANVGIAMGAAGTDAAIETADVALMRDDLLMVPEAIRLGRR
                     TLGIIRFNIAFALGIKAAFLALALLGCTSLWLAVLADTGATLLVIANAFRLLRR"
BASE COUNT          514 a          747 c          749 g          410 t
ORIGIN      
        1 ccaccgcgta ggcaacctcc tgggagcagg cgttgagatc cttggaaatc tcggactttt
       61 tgacgttcgg aatgaactcg cgcgcggaat tgtaatcgaa aaataccgcc atggcttcct
      121 cgggggtgga atccaccatg cgatacaccg cgatgcgcgg ccagggaaac cccttgacct
      181 cctcggtaac cagcaccggc tcgccgcgct caagcgtgcg gagctctttc gaatccagcg
      241 aatccacgag tcccgcctgg agagagtttg ccaagagaaa accgaggacg aaggcaactc
      301 cgggaaattt cactccgcga tgttgccgga atgccagcaa ggattccagc tcatttgtgc
      361 gaaatttcca ctcccgccag ccggcgagca gggtgagccc gagcaggagg gtctcctgca
      421 aaaaaagttc cacggcgccg ccgagcgccg gcccaaaaca gacgcagaca atttcccgcc
      481 cgcgcaacac ggcctggacc aggccgatga aaaaaagcag ttgcagcagg cccgcccccc
      541 ctagcgccgc gaaacgcagc cggggaacca agagggaaac gcccagcccg atttcaatcc
      601 acggcagcgc ttcggcgatc gcgtaagccg gattggcggc gaaaagtccg tattgttgaa
      661 tccccgcaaa cgtccgctcg gaatgcccaa gcttgctggc ccccgccagc aggaaaatga
      721 gcgcgagccc gccggccagc cagggatgct tgaaaagaaa ggctttcatg ccggccgaaa
      781 accgcgcgcg gcgccgggat ggcgatggcg ccatccattg atcctgattt tactttgccg
      841 gtgtaaaatg cgcacgaagc cgagagttgc tctcaacaaa ccccaacccg gggatttttc
      901 caccgttaat tcccggcaaa tcccaatcgc ggcgcttgct tgctagccgg gggcggcggg
      961 aatcccgaac agatcgtcca cgtaagcgcg ggcgcgctcg agattaagaa caatgatccg
     1021 cggatcctgc cgggccttgg cgacgagcat ggagccttgc cacaggccgt tgagaaacca
     1081 ggccagctcc tcggaatcga aatcggtttt gggcggctgc gcggccttgg cttcatcgag
     1141 caattggcgc gtgaacgccg tccaatcccc cagataagtc gcgcaggcct gctgtagcac
     1201 gggattggcc agggacattt cctgggaaat gatccccacc acgcaagtca ccgggcccgg
     1261 cgtctcgcgg acaaaaccaa tcatgatatc gaaaaaccgg tggagatggt cgagcgggtg
     1321 gcgggccggc tggcttttgg ccgcagcgta aagatccatg ccgaaatgcg cccaatccgc
     1381 caaggcggct tggccgagtt catccttgga ttggaaatga tggaaaaatg cgcccttggt
     1441 gacgcccgcc atccggcaaa tttgatccac cctcgtgccc gtgaagcctt gtgtcaggat
     1501 caggtcccgg gaggcatcca gaagtttgcg ccgggtctct tcggggcggc gggaattcaa
     1561 aacaacttca ctcatacaaa ctggttggta tgatactgag caagcggtaa gaatcaagtg
     1621 attatttagg gccggcggat tccgcggagg ccgggggggc caaggatgaa ccttgcgaag
     1681 cggcaggaat tcgtgcccaa atgggtgatc caatgactcc cgtctccgcc gccggcggtc
     1741 ctgccgggct tgaactcccc tcgcctcccg cggttatcgc cgaaggaggc ggaaggcatt
     1801 cgcgatgacg agaagggtcg ctcccgtatc ggcgagcacg gcgagccaaa ggctggtgca
     1861 gccaagcagg gcgagggcaa ggaaagcggc tttaatgccg agagcgaacg cgatgttgaa
     1921 acggatgatg cccaaagtcc ggcggccgag acggatggcc tccggcacca tcagcagatc
     1981 gtcgcgcatg agcgcgacgt cggcggtttc gatcgcggcg tcggtgcccg ccgcacccat
     2041 cgcaatgccg acattggcgg ccgccagcgc gggcgcgtcg ttcacgccgt cgccaaccat
     2101 gccggcgccg ccgtaatcgg cggcgagttt cctgacctgc tcgattttat tttccggcaa
     2161 aaggtcgccg tgggcttcgt caattcccgc ctcccgggca atggcattgg cggtgcgggc
     2221 attgtccccg ctgagcatga cgaccttgcg aatgccggaa gcgtgcagcc gccggatggc
     2281 ttcggccgca ttcgggcgga tggcgtcccc gaccgtgaga atcccaagaa cttcccccgc
     2341 gcaaccggca tgcggacgat ggccgacgac cacgacggat ttcgcgccgg cctcgatctc
     2401 ggcgagcacg gtttcgagtt
//
LOCUS       DATDAY010000020         3868 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_275531, whole genome shotgun sequence.
ACCESSION   DATDAY010000020 DATDAY010000000
VERSION     DATDAY010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 3868)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3868)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3868
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_275531"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..429
                     /locus_tag="VIS74_00395"
     CDS             <1..429
                     /locus_tag="VIS74_00395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5791723.1"
                     /translation="PTPMRAVRTLAVNNFKNTTWLPRVDVLLADTLVKQLQQDGTYTI
                     AGDNVADAILNCTLTRAEDRSIRSVTTNVLATSEFALLLVVSYEVQDRVTGAVLLSGT
                     ARGETTYYPTGDLTTDRRQAVSVAAERLSSNLSVQITEGW"
     gene            437..1132
                     /gene="rsmI"
                     /locus_tag="VIS74_00400"
     CDS             437..1132
                     /gene="rsmI"
                     /locus_tag="VIS74_00400"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEY5791724.1"
                     /translation="MLYVVATPIGNLGDLTFRALEALRGVDFIAAEDTRHSSRLLSHY
                     EIRKPLVSLHDHNESQRVPELAARLEQGENAALISDAGMPLISDPGFRLLQECIARGL
                     PYEILPGPSAVTTALAGSGLPCHAFFFGGFLPQKSGRRARELERALARDCTSIYFESP
                     HRLVKSLAALAESAPDRRACVARELTKTFQEYRKGSVSELQAHYEAHPPKGEITLLVA
                     PASGKEPADPEED"
     gene            complement(1167..2171)
                     /locus_tag="VIS74_00405"
     CDS             complement(1167..2171)
                     /locus_tag="VIS74_00405"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEY5791725.1"
                     /translation="MSKSYSIAIVGATGAVGMEMLHVLERRNFPVRELRLLASAQSAG
                     KPIWFRDKELVVQALTVESFAGIDIALFSAGSTISHQFAHVAVQSGATVIDNSSAFRM
                     DGHVPLIVPEINPHDARSNNGLIANPNCTTAITLMGLYPLHKEFGVRRVIASSYQAVS
                     GSGARGIEELRRQTHDLAVGHALHRTVYPHQIAFNVIPQVDSFLPGGYTKEEMKMQNE
                     GRKIMHLPDFAASVTCVRVPVYRAHSVAVTAQFERVPDIDLARQAIAAFPGVQLYDDP
                     VAGVYPMPLDCAGQDDCRLGRLRTDCALENALSFWICGDQILKGAALNAVQIAELLTH
                     "
     gene            2228..2737
                     /locus_tag="VIS74_00410"
     CDS             2228..2737
                     /locus_tag="VIS74_00410"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HEY5791726.1"
                     /translation="MKSLHPDSANPAGSADSAPPRTGIVVDIPTQTLRLEGEGLTPET
                     FSISTSKFGIGTEPGSCRTPLGRFEIAEKIGQGQPAGMRYQSRIPTGEILDQGGEEDH
                     VLTRILWLSGLEAENANTRGRYIYIHGTNQEALLGAPASHGCIRMRNADILRLFDAVA
                     QGTPVQILA"
     gene            complement(2998..3600)
                     /locus_tag="VIS74_00415"
     CDS             complement(2998..3600)
                     /locus_tag="VIS74_00415"
                     /inference="COORDINATES: protein motif:HMM:TIGR00567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HEY5791727.1"
                     /translation="MDLYRASDRTLIPRSFYERDPVTCARELIGTVLSWDGCRGRVVE
                     TEAYDSEGDEACHTFFKPSARAFVQAHPPGTAYVYLNYGVHWMLNVLVKGDRHGFVLF
                     RALEPLSGLAEMRRRRGTMPVEKLCSGPGKLTQALGIDGRAHGADLCADGARGFHFAS
                     EAPAVRADGRIGINRAVHFPWRFTLANHPHISARVKSPIA"
     gene            complement(3567..>3868)
                     /locus_tag="VIS74_00420"
     CDS             complement(3567..>3868)
                     /locus_tag="VIS74_00420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HEY5791728.1"
                     /translation="TAVGGPVHLAPGGPAQTRRVALVTGGAASEVAEAARAGADAFIT
                     GEGAHWTYPAAEELGVNLFYAGHYATETFGVRALAAHLEERFALPWTFIEHPTGL"
BASE COUNT          742 a         1265 c         1171 g          690 t
ORIGIN      
        1 ccgaccccga tgcgggcggt ccgcacgctt gcggtcaaca attttaagaa taccacctgg
       61 ctgccgcgcg tggacgttct gctggcggac acgctggtca aacagcttca acaagacggc
      121 acctacacca tcgccggcga caatgtggcc gacgccattc tgaattgcac cctgacccgc
      181 gcggaggatc ggtccatccg ttcggtcacc accaatgtgc tggcgacgag tgaattcgcg
      241 ctgctcctgg tcgtgagtta cgaggtgcag gaccgtgtca ccggcgccgt gctgctctcc
      301 ggcaccgccc gcggggaaac cacctattat cccaccggcg atctgaccac cgaccggcgc
      361 caggcggttt ccgtggcggc cgagcggctt tccagcaatc tgtccgttca aattaccgaa
      421 ggctggtaat cctcccttgc tctacgtcgt cgccacgccg atcggcaatc tcggagacct
      481 caccttccgg gcgctcgaag cgttgcgggg agtggatttc atcgccgccg aggacacccg
      541 gcattcctcc cggctgctca gccattacga aattcgcaag ccgctcgtca gcctgcatga
      601 tcacaacgaa agccagcggg ttccggaatt ggccgcccgg ttggagcagg gggaaaatgc
      661 ggccctgatt tccgacgcgg ggatgccgct gatttccgat ccgggattcc gcctcttgca
      721 ggagtgcatc gcgcgcggcc tgccttacga aatcctgccc ggcccctccg cggtcaccac
      781 cgcgctggcc ggttcgggac tcccctgcca cgcttttttc tttggcggat ttctgccgca
      841 aaaatccggc cggcgggcgc gggaactgga acgggcgctc gcccgggatt gcacctcgat
      901 ttatttcgag tcgccgcacc gcctggtcaa aagcctggcc gcgctggcgg aatccgcccc
      961 ggatcggcgg gcctgcgtcg cgcgggagct caccaaaacc tttcaggaat accggaaggg
     1021 ctccgtgtcc gagctccaag cccactacga agcccatccg cccaagggcg agatcacgct
     1081 gctcgtcgcc ccggcatccg gaaaagaacc agcggatccg gaggaggatt agtttcgctc
     1141 cccccccgcc gccggttcat cccgcgttaa tgcgtgagca gctcggcgat ctgcaccgcg
     1201 ttgagggcgg cgcccttgag gatctgatcg ccgcaaatcc agaaggacag cgcgttttcc
     1261 agggcgcaat ccgtgcgcag gcgccccaag cggcagtcgt cctgccccgc gcaatccaac
     1321 ggcatgggat acacccccgc caccggatca tcgtagagct gcaccccggg gaaggcggcg
     1381 atcgcctgcc gggccagatc aatgtccgga acccgctcga attgcgccgt gacggccacg
     1441 gagtgcgccc gatagaccgg aacccgcacg caggtcaccg aggccgcgaa gtccggcagg
     1501 tgcatgatct tgcggccttc gttctgcatt ttcatttcct ccttggtgta accccccggc
     1561 agaaaggaat ccacctgcgg aatgacgtta aaggcgattt ggtgcggata aaccgtccga
     1621 tgcagcgcat ggcccacggc gaggtcgtgc gtctggcgcc ggagctcctc gatgcctctc
     1681 gctccggaac cggaaaccgc ctggtagctg gacgcaatca cgcgccgcac cccgaattcc
     1741 ttgtgcaggg gataaagccc catcaacgta atcgccgtgg tgcaattggg attggcgatc
     1801 aagccgttat tggaccttgc gtcgtgcgga ttgatttccg gaacgatcaa cggcacgtgg
     1861 ccgtccatcc gaaaagccga ggaattgtcg atgacggtgg ccccgctttg cacggccaca
     1921 tgggcaaatt gatgggaaat ggtgctgccc gcgctgaaaa gcgcgatatc aatgccggcg
     1981 aaggactcca ccgtcagggc ctggaccacg agttccttgt cccggaacca aatcggcttg
     2041 ccggcggact gagcggaagc cagcaggcga agttcgcgga cgggaaaatt tcggcgctca
     2101 agcacgtgca gcatttccat gccgaccgca cccgtagcgc ccacaatggc aatcgaataa
     2161 cttttgctca taaatttgaa gagggggtta aaatctgcca gattcgatcc ggcgccgcaa
     2221 aaagaaaatg aaatcactcc atcctgattc cgcgaatccc gccggctccg ccgattccgc
     2281 tcccccgcgg accggcattg tcgtggacat tcccacccag acccttcgat tggaagggga
     2341 gggtctgacg ccggagacgt tctccatttc cacctcgaaa ttcggcattg ggaccgaacc
     2401 gggaagctgc cgcaccccgc tgggccgctt tgaaatcgcg gaaaaaatcg gccagggcca
     2461 gcccgccggg atgcgttacc agtcgcgaat tcccaccggg gagatcctgg accagggcgg
     2521 cgaggaggat cacgtcctca cccgaatcct gtggctgagc ggattggagg cggaaaacgc
     2581 caacacccgg ggccgctaca tttacattca tggcaccaat caggaagcgt tgctcggcgc
     2641 cccggccagc catggctgca tccgcatgag gaacgcggac atcctccggc tctttgacgc
     2701 ggtggcgcaa ggaacgccgg tgcaaattct tgcctgattc cttggaaaaa cttgtctccc
     2761 acgtgactta tgctcttatg aaggcgtgca gcctgcaaag aaaggagggg aaaatcctgt
     2821 gaagtttgtc aaatttttgg cgatcgccgc gtttgcggtg tccgctttga gcctcggcgc
     2881 ttgcgccagc aaaaaacccg cgcctgctcc tagcagcgtc ggatattcca agtaagcctt
     2941 tcggctgaaa gaattttagg ccaggcgggg gttgctctct gcctggcctt ttttcgttca
     3001 ggcgatgggg gatttcaccc gcgcgctgat gtgcggatgg ttcgcgaggg taaaccgcca
     3061 gggaaaatgg acggcccgat tgatgccgat gcgcccatcg gcccgaaccg ccggcgcttc
     3121 ggacgcgaaa tgaaaaccgc gcgcgccgtc cgcgcagaga tcggcgccat gtgcgcggcc
     3181 atcaatcccc agcgcctgcg tgagcttccc gggcccggaa cacaattttt ccaccggcat
     3241 cgttccccgg cgccggcgca tttccgcaag gccggacagc ggctcaagcg cgcggaagag
     3301 gacaaacccg tgccggtctc ccttcaccag gacattgagc atccagtgaa ctccgtaatt
     3361 caggtaaacg taggccgtcc cggggggatg cgcctggaca aacgcgcggg cgctgggttt
     3421 gaagaacgtg tggcacgcct cgtcgccttc ggaatcgtag gcctcggttt ccaccacacg
     3481 gccccggcag ccgtcccagc tcagcacggt tccgatcagc tcccgcgcgc acgtcaccgg
     3541 atcccgttcg taaaaggaac gcggaatcag agtccggtcg gatgctcgat aaaggtccat
     3601 gggagagcaa accgttcctc cagatgcgcg gccagggcgc ggacgccaaa cgtctccgtg
     3661 gcgtaatggc cggcgtagaa aaggttcacg ccgagttcct cggcggcggg atacgtccaa
     3721 tgtgcgcctt cgccggtaat gaaggcatcc gccccggccc gggcggcttc cgccacctcc
     3781 gaggccgctc cgccggtgac cagcgccacc cgccgggttt gtgcggggcc gcccggcgcc
     3841 agatgcacgg ggccgcccac ggcggttt
//
LOCUS       DATDAY010000021         3316 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_267700, whole genome shotgun sequence.
ACCESSION   DATDAY010000021 DATDAY010000000
VERSION     DATDAY010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 3316)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3316)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3316
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_267700"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..312
                     /locus_tag="VIS74_00425"
     CDS             <1..312
                     /locus_tag="VIS74_00425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5791729.1"
                     /translation="AESKKKSWFSNLFSWMKRKPAPPKALPLNAVGRVFVFNADQKYA
                     VIESGGASSLAPGTPLAVLADGNIKARLVVSSQSRPPFLIADWRSGHPQKDDPVYIVQ
                     E"
     gene            416..889
                     /locus_tag="VIS74_00430"
     CDS             416..889
                     /locus_tag="VIS74_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006983257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEY5791730.1"
                     /translation="METDTHPTALRQDQIERLADVIFFLQRNLVLTLSEGLGKNQISF
                     PQFFLLAHINASARPLTMTEIAERMHHTTAAATGLVDRLENLGYLRRTSAENDRRKVL
                     VSITAKGAELVNEMKNDISEKVSQLCSSILTPEQQHSWLEIYEKIFNYCSQKCCN"
     gene            894..2837
                     /locus_tag="VIS74_00435"
     CDS             894..2837
                     /locus_tag="VIS74_00435"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEY5791731.1"
                     /translation="MRYWTSLTLALVFVPAELRAQSASPTPAPLPTPNVAVPLPTPLP
                     PLEITPMSTPLPPATAAPPPTDLPASPAPTPTPTPGVTPVDTNVNPSAVPTPSVPTIP
                     IPVPYQASDLTPAPSDTQLPDVLMDQPLIPQNLLSPLPKAGTYQDTSGSIKTSEQTLS
                     NLRAAANKKDATILPNAPKLTLNDAVVYALKHNPDVLNAIEQIRLTRGQVVTVTSQAL
                     PQLTLSSGYTVQQQDLAVTTPQNQAWNVQFGVSQLLFDGGNVISGIRAAKFVQDSSYF
                     QLRNTIDTLIANVKSNFYQVILNRSLILAQEESVQLLESQLQDQINRYEAGTVPRFNV
                     LQAEVQLANAIPPLIEARNALRISQYQLVQLLGMDYPSGRQDVVPFNVVGDLQPLIRA
                     INTEQSIRTALSRGPGLKAQRQNILAQAQNVNVAISGYLPKVSANGGYLWQNDSLSEQ
                     LTDVVNGWFFGVQGSWAIFDGFLTTGNVMQAKAQLQTAKINYDNSVRQLILTVQQAIS
                     NLQTAQETLSSQQASVAQATEALRLSRERLDAGAGTQLDVLNAQVQLLQAQTTELQAR
                     YDYINFTAQYDAALSIDTKYLEEFDDPLTRYEKRKFQLANNQQVPQPALPRVYRKDDP
                     LARALGTPEPSPAKPSKSKTSAK"
     gene            2834..>3316
                     /locus_tag="VIS74_00440"
     CDS             2834..>3316
                     /locus_tag="VIS74_00440"
                     /inference="COORDINATES: protein motif:HMM:NF014619.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="laccase domain-containing protein"
                     /protein_id="HEY5791732.1"
                     /translation="MTAPGCRIEGFSALAALSFIRAAFLGHSAGVDVRTDRATALARL
                     AGPHRQAAESAGFSFSRLATAEQIHGAGVALAETPGHYEGVDGLITAQPGLPLGIYVA
                     DCAAVYLADRRSQAIGLVHSGKKGTELGIVPAAIRAMETHFGTRPADLTVQVSPCIRP
                     P"
BASE COUNT          653 a         1207 c          872 g          584 t
ORIGIN      
        1 gcggaatcga aaaagaagtc ctggttttcc aatctgttca gctggatgaa gcggaagccg
       61 gcacccccca aggccttgcc cctcaatgcc gtcggccggg tctttgtgtt taatgccgat
      121 cagaaatacg ccgtcatcga aagcggcggg gccagcagtc tcgcgcccgg aactccgctg
      181 gccgtccttg ccgatggaaa catcaaggcc cggctcgtgg tttcctcaca atcccgcccg
      241 ccctttctga tcgcggattg gagatcgggc cacccccaga aagacgatcc ggtttacatt
      301 gtgcaggaat agtgccataa ccgctcgatc gcccgaaccg gccgcacttc accctaactt
      361 tcaaaagatt gacaaatagt aaagtaagtt tataatcaag agccataaaa tatttgtgga
      421 aacggacact caccctactg cactgaggca ggatcaaatc gaacgccttg ctgacgtgat
      481 ctttttcctc cagcgcaacc tcgtgctgac gttgtccgaa gggcttggca agaaccagat
      541 ttccttcccc cagtttttcc tgctggcgca catcaacgcc tccgcgcggc cgctgacgat
      601 gacggaaatc gcggagcgga tgcaccacac gaccgccgcc gccacgggct tggtggaccg
      661 cctggaaaat ctcggttatc tccgccgcac cagcgccgag aacgaccgcc ggaaggtgct
      721 ggtttcgatc accgccaagg gcgcggagct cgtgaatgaa atgaagaacg acatttccga
      781 gaaagtttcg cagctttgct cctccattct cacccccgag caacagcatt cgtggctgga
      841 aatctacgag aaaatcttca attactgctc ccagaagtgc tgcaattaat cttatgcgtt
      901 attggacctc gctgaccctt gccctcgttt ttgtgccggc tgaacttcgc gcgcaatccg
      961 ccagcccgac tcccgccccg ctgcccacgc ccaatgtggc ggtgccgctg cccactcccc
     1021 tgccgccgct ggaaatcact ccgatgtcca cgccgctgcc tccggccacc gccgcgcctc
     1081 cgcccacgga tctgccggct tcgcccgcgc ccaccccaac ccccacgccc ggcgtgactc
     1141 cggtggacac caacgtcaat ccttccgccg tgccgacgcc ctcggtgccc accattccga
     1201 ttccggtgcc gtatcaagcg tcggacctga ccccggcccc gtcggacacc cagctgccgg
     1261 atgtgctgat ggatcaaccg ctcatcccgc agaatctcct ctcgccgctg cccaaggccg
     1321 gcacctatca ggacacttcg ggctccatca aaacctccga acagacgctc agcaatctgc
     1381 gggccgccgc caataagaag gacgccacga ttttgccgaa tgcgcccaaa ttgacgctca
     1441 acgacgcggt cgtttacgcg ctcaagcaca atccggacgt gctcaacgcc atcgagcaaa
     1501 tccgcctgac ccgcggccag gtggtgacgg tgacctcgca ggcgctgccg cagctgaccc
     1561 tcagctccgg ctacaccgtc cagcaacagg atctcgcggt caccactccg cagaatcagg
     1621 cgtggaacgt gcagttcggc gtcagccagc tgctcttcga cggcggcaac gtcatctcgg
     1681 gaatccgggc cgcgaaattc gtgcaggact cctcctattt ccagctgcgg aacaccatcg
     1741 acacgctcat cgcgaacgtg aagtcgaatt tctaccaggt catcctcaac cgctcgctca
     1801 ttctcgccca ggaggaatcc gtgcaattgc tcgagagcca gctgcaggat cagatcaacc
     1861 gctacgaagc cggcaccgtc ccgcgcttca acgtcctgca ggccgaggtc cagctcgcca
     1921 acgcgattcc gcccttgatc gaggcgcgca atgccctgcg catttcgcaa taccagctcg
     1981 tccaactgct cggcatggat tatccatccg gccggcagga tgtcgtgccc tttaacgtgg
     2041 tcggcgacct ccagcccctg atccgcgcga tcaataccga gcaatccatc cgcaccgcgc
     2101 tcagccgcgg ccccggcctc aaggcccagc gccagaacat cctcgcgcag gcgcagaatg
     2161 taaacgtcgc catctccggc tacctgccga aagtcagcgc caacggcggt tacctctggc
     2221 agaacgacag cctctccgag cagctcaccg acgtggtcaa cggctggttc ttcggcgtcc
     2281 agggttcgtg ggcaatcttt gacggtttcc tgaccaccgg caacgtcatg caggccaagg
     2341 cccagctcca gacggccaaa atcaattacg acaattccgt gcgccagttg atcctcaccg
     2401 tccagcaggc gatctccaat ctccagaccg cgcaggaaac cctctccagc cagcaggcca
     2461 gcgtggccca ggccaccgag gcgctgcgcc tctcgcgcga gcgcctcgac gccggcgcgg
     2521 gcacccagct cgacgtgttg aacgcccagg tgcaattgct gcaggcccag accaccgaac
     2581 tgcaggcgcg ttacgattac atcaacttca ccgcccagta tgacgcggcg ctctcgatcg
     2641 acaccaaata cctggaggaa tttgacgatc cgctcacgcg ttacgagaag cgcaaattcc
     2701 agctggcgaa caatcagcaa gtcccgcagc ccgccctgcc gcgcgtttac cgcaaggacg
     2761 atccgctcgc ccgcgcgctc ggcactcccg aaccgtctcc cgccaagccg tccaagtcaa
     2821 aaacctcggc aaagtgacgg ctcccggctg ccggatcgag ggtttttccg cgctcgcggc
     2881 gctttccttc attcgcgcgg ccttcctcgg gcacagcgcc ggggtggacg tccggacgga
     2941 tcgcgccacc gcgctcgccc gtctcgccgg gccgcaccgc caggccgccg aatcggccgg
     3001 cttctccttt tcccgtctcg ccaccgccga acaaatccac ggcgccggcg tcgcgctcgc
     3061 cgaaacgccc gggcattatg aaggcgtgga cgggctcatc accgcgcagc ccgggcttcc
     3121 gctcggcatc tacgtcgccg actgcgccgc ggtttatctg gcggaccgcc gcagccaggc
     3181 catcgggctc gtccattccg gcaaaaaagg caccgaactc ggcatcgtcc ccgccgccat
     3241 ccgcgcgatg gaaacgcatt tcggcacccg gcccgccgat ttgacggttc aggtcagccc
     3301 ctgcatccgc ccgccg
//
LOCUS       DATDAY010000022         4937 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_2557447, whole genome shotgun sequence.
ACCESSION   DATDAY010000022 DATDAY010000000
VERSION     DATDAY010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 4937)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4937)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4937
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2557447"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..517
                     /locus_tag="VIS74_00445"
     CDS             <1..517
                     /locus_tag="VIS74_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006983850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEY5791733.1"
                     /translation="NYVTAEVGVPTLQDILAELSKPGRDPRKKFEAFAFADGVSKPQD
                     LKPGMKLPGIVTNVTNFGAFVDVGVHQDGLAHISQLSDSFVNNPADVVKVGQKVNATV
                     LEVDLERKRIALSLKSAPDLSGASRGPRGNNGPAPRHDSAPKKPAQSGGSFGGDWFSQ
                     AMAKTQPAKKR"
     gene            complement(568..891)
                     /locus_tag="VIS74_00450"
     CDS             complement(568..891)
                     /locus_tag="VIS74_00450"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEY5791734.1"
                     /translation="MKRDEIYWVTLDPVAGSEIGKTRPAVIVSMDALNRLVETVVVCP
                     LTSQLHPEWRTRLPVKVAGKPAEIAADQIRTVSKGRLGQKLGVLTARDAAALRRLLGE
                     MYAGV"
     gene            complement(881..1168)
                     /locus_tag="VIS74_00455"
     CDS             complement(881..1168)
                     /locus_tag="VIS74_00455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HEY5791735.1"
                     /translation="MKITVLKVSRIGNSRGIRLPAEMLRKYHITDAVIAEENADEIVL
                     RAVRTNRRKLSWDDTAKAIAAAGEDWSEWEGALADGLDQVPWEKTPPGDEA"
     gene            1349..2536
                     /gene="fabV"
                     /locus_tag="VIS74_00460"
     CDS             1349..2536
                     /gene="fabV"
                     /locus_tag="VIS74_00460"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004318 - enoyl-[acyl-carrier-protein]
                     reductase (NADH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabV"
                     /protein_id="HEY5791736.1"
                     /translation="MIISPKIRGFICTTAHPDGCAAHVQGQINYVKSKPPLANAPKRV
                     LVVGSSTGYGLASRIVSAFGGGAATIGVFFEKEAADKKTASAGWYNSAAFETAAAQEG
                     LYARSFNGDAFSSEMKQRVIEAIQTDLGQVDMIVYSLASPRRTDPVTGEVYKSVLKPT
                     AAAFTNKNLNTDKKEIETVTIEPATEEEIAQTVKVMGGDDWKLWIDALSAAGALAEGF
                     KTVAYSYIGPELTWAIYTDGTIGRAKVHLEETAKELNGQYGAGTALVSVNKALVTQAS
                     SAIPVVPLYISLLYKVMKAKGLHEGCIEQIHRLFAGHYGSATGPKLDDKGRVRIDDWE
                     MRGDVQSEVAQVWNSVTTENLDELSDFAGYQKEFYRLFGFGLAGVDYAAETDPARPIP
                     SLS"
     gene            2582..3145
                     /locus_tag="VIS74_00465"
     CDS             2582..3145
                     /locus_tag="VIS74_00465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5791737.1"
                     /translation="MSQRLLPGILALGLALVSAQAQDAPSNSAVAALAAIPPQYTGQI
                     LQLTGDKGTPEPAQWVIYARVRDDDDSSPSTVHTIVVAAGQVISDNPAANLFEVFRNT
                     GYFGAPDIQVDSGDAFLTLTLYATSNNKVVNAISYKLVQSGDGVPIWTLTAFDPNGGE
                     IGWLRILATTGAVIGQKGFPVTPAIPN"
     gene            3210..4595
                     /locus_tag="VIS74_00470"
     CDS             3210..4595
                     /locus_tag="VIS74_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HEY5791738.1"
                     /translation="MSPLSELEAALTQALGARGVKKGAAAAPYGGDKWFAHRQPDFAV
                     LPRTAEQAAGVLKIATQFRVPVTPRGAGYGYVGGCVPEQGGIVLSTEKMNRILELNDE
                     DFVAVVQPAVITGELQAAARMAHLFYPPDPASLKNCSIGGNIATNAGGPRCLKYGTTR
                     HYVLGLQVALMDGTLVRLGGRTHKNKTGFDLAGLFVGSEGLLGIVTEATLRLLPLPTG
                     RAALSAGFTDMRHAAEAVQAIFHAGFLPAAVEIADSFTLQAARAAMPNARRIPPGDAH
                     LLVEIDGQRDSVRLEILALQKVVSRLKPTAVKIAKTPAECEKLWDLRRGFSQSLKSTG
                     LTKLNEDIVVPRGKLVDLIAFARQLQQKSGFPIACFGHAGDGNIHVNIMVEGIDAPGV
                     RAAADQVLDELFRQILAWGGAITGEHGIGLAKLPWWPQAANADLRRVHHAIKSALDPH
                     QLLNPGKFGGA"
     gene            4605..>4937
                     /locus_tag="VIS74_00475"
     CDS             4605..>4937
                     /locus_tag="VIS74_00475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5791739.1"
                     /translation="MNPRFLVQAAAGALSNLGIFGLLLFLPAGTLDWWRAWVFLGIVT
                     AASVATLLLVFRNNEELLRERMKPLIQKGQPLSDRLIVIPLVLSFLGIIALIPVDVFH
                     LHLLPRPDP"
BASE COUNT          920 a         1689 c         1463 g          865 t
ORIGIN      
        1 aaactacgtc accgccgagg tcggcgtgcc cacgctgcag gacatcctcg ccgagctgtc
       61 caagcccggc cgcgacccgc gcaaaaaatt cgaggccttc gccttcgccg atggcgtctc
      121 caagccgcag gatctcaagc ccggcatgaa acttcccggc atcgtcacaa acgtcaccaa
      181 cttcggcgcg ttcgtggacg tcggcgtcca ccaggacggc ctggcgcaca tcagccaact
      241 ctccgacagc ttcgtgaaca accccgccga cgtcgtgaag gtcggccaga aggtgaacgc
      301 caccgtgctc gaagtggatt tggaacgcaa acgcatcgcg ctttcgctca agtccgcgcc
      361 cgacctcagc ggcgcgtccc gcgggccgcg cggcaacaac ggcccggccc cgcgccacga
      421 ttccgcgccg aagaaacccg cccaatccgg cggcagcttc ggcggcgact ggttcagcca
      481 ggcgatggcc aagacccagc cggcgaagaa gcgttgattt ttcaagcgcg cgacgccgat
      541 ggacgaatgc caatccggct tgccttttca gacgccggca tacatctctc cgagcaatcg
      601 ccgcagggcc gccgcgtcac gggccgtcaa cacgccgagc ttttggccga gccggccctt
      661 gctgacggtg cgaatttggt cggcggcaat ctccgcgggt tttccggcaa cctttaccgg
      721 caaacgcgtg cgccactcgg gatgcagctg gctcgtgagc gggcagacca ccaccgtctc
      781 caccaggcga ttcagcgcgt ccatgctgac aatgacggcg gggcgcgtct tgccgatttc
      841 gctccccgca accggatcca gcgtcaccca ataaatctca tcacgcttca tctccgggcg
      901 gcgtcttctc ccagggaact tggtccagtc catccgccag cgcgccctcc cactcggacc
      961 aatcctctcc agccgcggcg atggccttgg cggtgtcgtc ccaggacaat tttcgccggt
     1021 tcgtccggac ggcgcggagg acgatttcat ccgcattttc ctcggcgatg accgcatcgg
     1081 taatgtgata tttgcgaagc atctccgccg gcaggcgaat cccgcgggaa ttgccgatgc
     1141 gcgaaacctt aaggacggtg attttcacgt aatcaccgta attacatcca ctccgcgggt
     1201 caatgggatt atggcggcgc gtccccgctc cgcgccgcgc ggcgatgccg gaaatcgagc
     1261 ggcttccatt agcggcctcc tcaaaaaaga aaactccgtt cggcgtcaaa ggcttgtctg
     1321 ccggcgggaa acccgtcaaa aatctcccgt gattatttct ccaaaaattc gtggcttcat
     1381 ctgcacgacg gcccatcccg acggctgcgc ggcgcatgtt cagggtcaga tcaactatgt
     1441 caagtcgaag cccccgctcg ccaatgcccc caaacgcgtg ctcgtggtgg gctcatcgac
     1501 gggctacggc ctcgcctcgc gcatcgtgtc ggccttcggc ggcggcgcgg cgaccatcgg
     1561 cgtctttttt gaaaaggaag cggccgacaa aaaaaccgcc tcggcgggct ggtataactc
     1621 cgcggccttc gaaaccgcgg cggcccagga gggtctttac gcccggagct tcaatggcga
     1681 cgcgttctcc agcgaaatga agcagcgggt cattgaggcc atccaaaccg acctcgggca
     1741 ggtggacatg attgtttaca gcctcgcctc cccccgccgc accgaccccg tcacgggtga
     1801 agtttacaag tcggtcctca agccgaccgc cgcggcgttc acgaacaaga atctcaacac
     1861 cgacaaaaag gaaatcgaga ccgtcaccat cgaacccgcg accgaggagg aaatcgccca
     1921 gaccgtcaag gtgatgggcg gcgacgactg gaagctatgg atcgacgcgc tgagcgccgc
     1981 cggcgcgctg gcggaaggct tcaagaccgt cgcgtattcc tacatcgggc ccgagctgac
     2041 gtgggcgatc tatacggatg gcaccatcgg ccgcgccaag gtccacctcg aagaaaccgc
     2101 gaaggaattg aacggccaat acggcgccgg caccgcgctg gtctccgtca acaaggcgct
     2161 cgtcacgcag gcaagctccg cgattcccgt cgtgcccctc tacatctcgc tgctttacaa
     2221 ggtcatgaag gccaagggcc tccacgaagg ctgcatcgag caaatccacc gcctcttcgc
     2281 cggccactac ggctcggcca ccggcccgaa gctcgatgac aagggccgcg ttcgcattga
     2341 cgattgggaa atgcgcggcg acgtgcagtc cgaggtcgcc caggtctgga actcggtcac
     2401 gacggaaaac cttgacgagc tctccgactt cgccggctac cagaaggaat tctaccgcct
     2461 cttcggcttc ggcctcgccg gcgtggatta cgcggcggaa accgatccgg cccggcccat
     2521 tccgagcctg agctaaaaat tggggattgc caccggcggg gaccgcgcgt ttgatcttgc
     2581 catgagtcaa cgacttctcc ccggcattct ggcccttggg ctggcgctcg tttccgcgca
     2641 agcccaggac gccccttcca acagcgcggt ggccgccctg gccgccattc ctccccaata
     2701 caccggacaa atcctccagc tcaccggcga caagggcacg ccggagccgg cccagtgggt
     2761 catctacgcc cgggtgcggg acgacgacga cagctcgccc tcgacggtgc atacgatcgt
     2821 tgtcgccgcc ggccaggtga tttccgacaa tcccgccgcc aacctgttcg aggtcttccg
     2881 caacaccggc tacttcggcg ctccggacat ccaagtggac tccggggatg cgttcctgac
     2941 cctcacgctt tacgccacct ccaataacaa ggtggtgaat gccatcagct acaagctggt
     3001 ccagtctggc gacggcgtcc ccatctggac cctgacggcc ttcgatccca acggcggtga
     3061 aatcggctgg ctgcgcatcc tggccaccac cggcgcggtg atcggccaaa agggttttcc
     3121 cgtgacgccg gcgattccga actaaaccgg attgactgga ttaaccgggt taacgggatt
     3181 tcattcatta aaaaatccgg tttcgttcca tgagcccact ttccgaactc gaagccgcgc
     3241 tgacccaggc gctcggagcg cgcggcgtta aaaagggcgc cgccgccgcg ccctatggcg
     3301 gcgacaaatg gttcgcgcac cgccagccgg atttcgccgt gctgccgcgg acggcggaac
     3361 aggccgccgg ggttttgaaa atcgccaccc aattccgcgt gccggtcact ccgcgcgggg
     3421 cgggttacgg ctacgtcggc ggctgcgtgc cggaacaggg cggcatcgtc ctgagcacgg
     3481 aaaaaatgaa tcgcatcctc gagctcaacg atgaggattt tgtcgcggtc gtccagccgg
     3541 cggtcatcac cggcgaactt caggccgcgg cgcgaatggc ccatctcttt tatccgcccg
     3601 accccgcgag cctgaagaat tgcagcatcg gcggaaacat cgccaccaac gccggcgggc
     3661 cgcgctgcct gaaatacggc acgacgcgcc attacgtgct cggcctgcag gtggcgctca
     3721 tggatggcac gctggtgcgc ctgggcggac ggacgcacaa gaacaagacc ggcttcgacc
     3781 tcgccgggct gtttgtcggc tcggagggcc tgctcggaat cgtcaccgag gccacgctcc
     3841 gcctgctgcc gctgcccacg ggccgagcgg ccctctccgc cggctttacg gacatgcgcc
     3901 acgcggcgga agcggtgcag gccatttttc acgcgggctt cctgcccgcc gcggtggaaa
     3961 tcgccgacag cttcacgctc caggccgcgc gcgccgcgat gcccaacgcc cgccgcatcc
     4021 cgcccggcga cgcgcatttg ctcgtcgaaa ttgacggcca gcgcgactcc gtgcgcttgg
     4081 aaatcctcgc gctgcaaaaa gtcgtgagcc ggctgaaacc caccgcggtc aaaatcgcga
     4141 aaacgccggc ggaatgcgaa aagctctggg atttgcgccg cggattttcc cagtcgctga
     4201 aatccaccgg cctcaccaaa ctcaacgagg acatcgtcgt gccgcgcggc aaattagtgg
     4261 acctcatcgc cttcgcccgc cagctgcagc aaaaatccgg cttcccgatc gcctgcttcg
     4321 gccacgcggg ggatggcaac atccacgtga acatcatggt cgagggaatc gatgcgcccg
     4381 gcgtgcgggc ggccgccgat caggtattgg atgaactctt ccggcaaatt ctggcctggg
     4441 gcggcgccat taccggcgag cacggcattg ggctggccaa gctcccgtgg tggccgcagg
     4501 cggcgaatgc ggacctgcgc cgcgtgcatc acgccatcaa gtccgccctc gatccgcatc
     4561 aactgctcaa tcccggcaaa tttggcgggg cctgagccgg catcatgaat ccgcggtttc
     4621 tggtccaggc cgccgcgggg gccttgagca atctcggcat tttcggactc ctcctgtttc
     4681 tccccgcggg aacgctggac tggtggcgcg cctgggtgtt tctgggaatc gtcaccgccg
     4741 cctcggttgc cacgctgctc ctggtctttc gcaataatga ggagctgttg cgcgagcgca
     4801 tgaagccgct cattcaaaag ggccagccgc tgtccgaccg gttgatcgtg attccgctgg
     4861 tgctgtcctt tttgggaatc atcgcgctga ttcccgtgga tgtgttccat cttcatttgc
     4921 tgccacggcc ggatccg
//
LOCUS       DATDAY010000023         5624 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_262864, whole genome shotgun sequence.
ACCESSION   DATDAY010000023 DATDAY010000000
VERSION     DATDAY010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 5624)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5624)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5624
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_262864"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..333)
                     /locus_tag="VIS74_00480"
     CDS             complement(<1..333)
                     /locus_tag="VIS74_00480"
                     /inference="COORDINATES: protein motif:HMM:NF013658.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase family
                     protein"
                     /protein_id="HEY5791740.1"
                     /translation="MDHLDELEAQSVYILREAYHSFSNLCMPWSMGKDSNVLIWLSKK
                     AFCGKIPYPVLHIDTTYEFPEMLKFREWARAEYGLDLIVKINEDARAGRGAYPTPIGY
                     ETHDPVTVT"
     gene            433..756
                     /locus_tag="VIS74_00485"
     CDS             433..756
                     /locus_tag="VIS74_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HEY5791741.1"
                     /translation="MAHAPIPAPRLQLIGTELAVAWRDGTESYFPLEFLRRACPCAAC
                     GGEPDVLGRIIRPRQELTEKSFQLAGYEFVGGYGWQPAWGDGHRSGIYSWDFLKRLEE
                     GMAGK"
     gene            753..1634
                     /locus_tag="VIS74_00490"
     CDS             753..1634
                     /locus_tag="VIS74_00490"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEY5791742.1"
                     /translation="MKPIQLTFLRRLGSTLTLWAVILAGIFAGYEIGFFILIAGLGLM
                     ALWEYFKMLGDARIPHFRLTGMWTGALFMTGGFLFCRQSGGAGYEFETAALAGFLIIA
                     FARQMFERARERESLEAIAYTVFGLLYIPWLFNFITKIVFLLPPLANGQPVGQYYVLY
                     LLLVTKFSDMGAYVFGMLFGKHPFAPHISPKKTWEGFAGALFTAMLGSFGLYFLMPQK
                     LAAFSLTDVAILGLLLGFAAVAGDLAESIVKRSTNAKDSSHLLPGIGGTLDLIDSLLF
                     TAPILFFYMRLVMGIGS"
     gene            1631..2770
                     /gene="dxr"
                     /locus_tag="VIS74_00495"
     CDS             1631..2770
                     /gene="dxr"
                     /locus_tag="VIS74_00495"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: protein motif:HMM:TIGR00243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HEY5791743.1"
                     /translation="MKPRNVVVLGATGSIGRSAQRVARHLGERMRLVGLSSNQNAAGL
                     AEAAREFNAAAVCLGDSEPVNGLPPGCRLLRGAEGLIELATLPEADLVLIAIVGTAGL
                     RPALAAIEAGKDIAVASKEILVAAGEIVMAAAARRGVKVLPVDSEHNAIFQCLEGRDP
                     ASVKRLILTCSGGPFRHTPAAEFGAITPESALKHPTWQMGRKITIDSATLFNKGLEML
                     EARWLFDLPMQQVDVIIHPQSLIHSMVEFVDGSVLAQLSPSDMAFPIHYAVTWPERVP
                     GAQQPLDFARLGSLEFFAPRWADFPALRLAREAGIRGGTLPAVLNAANEVAVEAFLDR
                     KIPFPRIWELVEQVMQSAPPTDHPTLAEIETADATAREHARAALA"
     gene            complement(2942..4222)
                     /locus_tag="VIS74_00500"
     CDS             complement(2942..4222)
                     /locus_tag="VIS74_00500"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY5791744.1"
                     /translation="MNTTYDAIIIGGGPGGSTAASALAQAGKKVLILERERFPRFHVG
                     ESLIPYGNDELKKIGIWDKLNEFGFMPKLGAEFVLGNSKAVTRIIFGRYLPASYAQTF
                     QVERSRFDHLLLDHAAASGGEVWQETLVNSVEVSGEGVAVNCSRQGESHTVRARWILD
                     ASGRDALLGKQLKLPKTDLGLPKKFATFAHFKGVIRNEPPADGHITVVRLDFGWCWMI
                     PLDAEKTSIGLVQTLEHFRSTGLKPGECFERVVATTPELRRRMGNAERVSDYSFAGDY
                     TYRHLQNAGPRWILIGDAAGFIDPIFSSGVMLAIKSGYLAASEILAADRAGRPLSAAA
                     QARYTRKVGKMCKVFLNMIKMFYSNHAFEVFMTPDPEKRMEWAVNNLVAGNTNLGWRL
                     RLRVWMFYGVCALQKYFPIVPKLDLGERPLGVQT"
     gene            4356..>5624
                     /gene="rseP"
                     /locus_tag="VIS74_00505"
     CDS             4356..>5624
                     /gene="rseP"
                     /locus_tag="VIS74_00505"
                     /inference="COORDINATES: protein motif:HMM:TIGR00054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HEY5791745.1"
                     /translation="MEIFKILFILLEVVLLFNLIIVVHELGHFLAARWRGLKVDKFAV
                     WFGKPLWKKTIGGVEYRLGSIPAGGFVSIPQLAPMEAVEGKPDQEASELPLAKPIDKI
                     IVAAAGPIFSLGLAVAFALIVWVIGRPVSEGETSTTIGYVLAGGPADKAGLRAGDRIL
                     SVNGNPVSRFSGMGNVEASIVWNIARSEEPSLPIEIERDGKTQTVWVEPSLPTRQGWS
                     RKNLRQIAIAPAQTPMIAKVIPGSPAAAAELRPQDLILAINGQKLLSLPGLAEYLNQY
                     SGGPVTLTVSRDGQTFEQPVTPQIPVDGDKPRIGIQWDDRGLTTLSHPNPIAQIVASA
                     ETMWATLTAIASPKSDIKIEHLSGPVGIMRLYYLIFEAPDGWRVALWFSVIFNVNLAI
                     LNLLPLPVLDGGHITLSLIELIRRRPLDIRL"
BASE COUNT          984 a         1844 c         1718 g         1078 t
ORIGIN      
        1 cgtgaccgtg accggatcgt gcgtttcgta accgatcggc gtcgggtagg cgccccggcc
       61 ggcccgcgcg tcctcattga ttttcacgat caaatcgaga ccgtattccg cccgggccca
      121 ctcgcgaaat ttgagcattt cgggaaattc gtaggtcgtg tcgatgtgca ggaccggata
      181 cggaatcttg ccgcaaaagg cttttttcga gagccagatc aggacgttcg aatccttgcc
      241 catgctccaa ggcatgcaga gattcgagaa gctgtgatac gcttcccgca aaatatagac
      301 gctttgcgcc tccaattcgt ccaaatgatc cattccagat agggttagtc gggattaagg
      361 acccggacaa tagaaaaaca ttcctttctg cttggagcaa gcgcgggatt cggggcagac
      421 tcaccgcgca aaatggccca cgcaccgatt cccgcccccc gcctgcaact catcggcacg
      481 gagctggccg tggcgtggcg ggacgggacc gagagttatt tcccgctgga atttctgcgg
      541 cgcgcctgtc cctgcgcggc ctgcggcggg gaaccggacg tgctgggccg gatcatccgg
      601 ccgcgccagg aattgacgga aaaaagcttc cagttggcgg gttacgaatt tgtcggcggt
      661 tacggctggc agccggcgtg gggcgacggg caccgctccg ggatttattc ctgggatttt
      721 ctcaagcgtt tggaagaggg tatggccgga aaatgaagcc gattcagctc acctttcttc
      781 gccggctcgg cagcacgctc acgctgtggg ccgtgattct cgcgggcatc ttcgccggtt
      841 acgaaatcgg ctttttcatt ctgatcgccg gcctggggct gatggccctg tgggaatact
      901 tcaagatgct cggcgacgcg cggatcccgc atttccgcct caccggcatg tggacgggag
      961 cccttttcat gacgggcggc ttcctcttct gccgccaatc cggcggcgcg ggttacgaat
     1021 ttgagaccgc ggcgctcgcc ggctttctca tcatcgcatt cgcgcggcag atgttcgaac
     1081 gcgcgcgcga gcgcgaatcg ctggaggcaa tcgcctacac ggtcttcggc ctgctctaca
     1141 ttccctggct tttcaatttc atcaccaaga tcgtgttcct ccttccgccg ctcgcgaatg
     1201 gccagcccgt ggggcaatat tacgtgcttt atctgctgct ggtgacgaaa ttcagcgaca
     1261 tgggcgcgta tgtcttcggc atgctcttcg gcaaacatcc cttcgcgccg cacatcagcc
     1321 cgaaaaaaac ctgggaggga ttcgccggcg cgctgttcac cgccatgctg ggcagcttcg
     1381 ggttgtattt cctgatgccc cagaagctgg ccgcgttctc cctcaccgat gtcgcgattc
     1441 tcggcctgtt gctcggcttc gccgccgtgg cgggcgacct cgcggaatcc atcgtcaaac
     1501 gcagcacgaa cgccaaggat tccagccacc tgctgccggg catcggcggc acgctggacc
     1561 tcattgacag cctgcttttc acggcgccga tcctgttctt ttatatgcgg ctggtgatgg
     1621 gaatcggatc atgaaaccgc gaaatgtcgt tgtcctcggc gccaccggct cgattggccg
     1681 gagcgcccag cgggtcgccc ggcacctcgg cgaacgcatg cggctggtcg gcttgtcctc
     1741 gaatcaaaac gccgccggac tggcggaagc cgcccgcgag tttaacgcgg ccgccgtttg
     1801 cctcggcgat tccgaaccgg tgaacgggct gccgcccgga tgccggctgc tgcgcggggc
     1861 ggaggggttg atcgagctcg ccacgctgcc ggaggcggac ctggtgctga ttgcgatcgt
     1921 gggaaccgcc gggctccggc ccgcgctggc ggccatcgaa gccggcaagg acatcgcggt
     1981 cgcgagcaag gagatccttg tcgccgccgg ggaaatcgtc atggccgccg cggcgcgccg
     2041 cggcgtgaag gttctgccgg tggacagcga gcacaacgcc attttccaat gcctcgaagg
     2101 ccgcgacccg gcttcggtca agcgactgat cctgacctgc tcgggcgggc cgttccgcca
     2161 cacgccggcc gcggagttcg gcgcaatcac ccccgaatcc gcgctcaagc atcccacctg
     2221 gcaaatgggc cgcaagatca cgatcgattc cgccacgctc ttcaacaagg gcctggaaat
     2281 gctcgaagcg cgctggcttt tcgacctgcc gatgcagcag gtggatgtca tcatccatcc
     2341 gcagagcctg attcattcca tggtcgaatt cgtggacggc tccgtgctgg cgcagctgag
     2401 cccctccgac atggcgttcc ccattcatta cgccgtcacc tggcccgagc gggtgccggg
     2461 cgcgcagcag ccgctggatt tcgcccggct gggttcgctg gaatttttcg cgccgcgctg
     2521 ggcggatttc cccgcgctga ggctcgcccg ggaggccggc atccgcggcg gcacgctgcc
     2581 cgccgtgctc aatgccgcca acgaagtcgc cgttgaggca ttcctcgacc ggaaaatccc
     2641 gtttccgcgg atttgggaat tggtggagca ggtcatgcaa tcggcgccgc ccacggacca
     2701 tcccacgctc gccgaaatcg aaaccgcaga cgccaccgcc cgggaacacg cccgggcggc
     2761 gttggcgtaa tttcaaccca aacccgttcg aggacgttcg ggttagcttg atgcggccga
     2821 aagcgacgga cggctcatcg agccgtccct gcccgggatg ggtggggcgg ggtcgataga
     2881 aggggggagg gatccattct cccctcccgc cgccgtgact ttttcccttc gcgctgaggt
     2941 gctaggtctg gacgcccagc ggccgctcgc ccaggtccag cttcggcacg atcgggaaat
     3001 atttctgcag cgcgcagact ccgtagaaca tccagacgcg caggcggagg cgccagccga
     3061 gattcgtatt tccggccacg agattgttca ccgcccactc cattcgcttt tccggatcgg
     3121 gggtcatgaa gacctcgaag gcgtggttgc tgtagaacat cttgatcatg ttcaggaaca
     3181 ccttgcacat cttgcccacc ttccgcgtgt agcgggcctg cgcggcggcg ctgagcggcc
     3241 tccccgcccg gtcggcggcg agaatctcgc tggcggcgag gtagccggac ttgatggcca
     3301 gcatgacccc ggaggaaaaa atcggatcga tgaaccccgc ggcgtcgccg atcaaaatcc
     3361 agcgcggccc ggcgttttgc aggtggcggt aggtgtaatc cccggcgaag gaataatcgc
     3421 tgacgcgctc cgcgtttccc atccgccgcc gcaattccgg cgtcgtggcg accacccgct
     3481 caaagcattc ccccggcttc aggccagtcg aacggaagtg ctccagcgtt tgcacgaggc
     3541 cgatggacgt cttctccgcg tcgagcggaa tcatccagca ccagccgaaa tccaggcgga
     3601 cgacggtgat gtggccatcc gccggcggct cgttgcgaat gacgcccttg aagtgcgcga
     3661 aggtcgcgaa ttttttcggc aggcccagat cggtcttcgg cagcttcaac tgcttgccca
     3721 gcaaggcatc ccgcccgctg gcgtcgagaa tccagcgcgc ccgcacggtg tggctttcgc
     3781 cctggcgcga gcagttcacg gccacgccct cgccggaaac ctcgacggaa ttcaccagcg
     3841 tctcctgcca gacttcgccg cccgaggcgg cggcgtgatc gagcagcaga tggtcaaagc
     3901 gcgaccgttc cacctgaaag gtttgcgcgt aggaagccgg cagataccgc ccgaaaataa
     3961 tccgcgtgac cgccttggag ttcccgagca cgaactcggc gccgagcttc ggcatgaagc
     4021 cgaactcgtt caacttgtcc cagatgccga tttttttcag ctcgtcgttg ccgtagggaa
     4081 tcagcgattc ccccacgtga aaccggggga accgctcccg ctcgagaatc aaaactttct
     4141 tgcccgcctg ggccagtgcg ctggccgcgg tgcttccgcc cggccctccc ccgatgatga
     4201 tcgcgtcgta ggtcgtattc attaatggta atgcgactca tggaaatgcg ccgtttttga
     4261 atcgcgaaca agccaaattc cggtttttcg ccgggcagga atttgcggcc ggcggttgat
     4321 ttttcgcgat ttccgggcat gctgtagctg ccgacatgga aattttcaaa atccttttca
     4381 tcctcctgga ggtcgttctc ctctttaatc tcatcattgt cgtccacgag ctggggcact
     4441 tcctggcggc ccgctggcgc ggcctcaagg tcgataaatt cgccgtctgg ttcggcaaac
     4501 ccctctggaa gaagacgatc ggcggagtgg aataccgtct cggctcgatt ccggccggcg
     4561 ggtttgtgtc cattccccag ctggcgccca tggaggcggt cgagggaaaa cccgatcagg
     4621 aagccagcga gctgccgctc gccaagccga ttgacaagat catcgtcgcc gccgcggggc
     4681 cgattttcag cctcgggctg gccgtggcct ttgcgctgat cgtctgggtc atcggccgcc
     4741 cggtcagcga aggcgagacc agcaccacca tcggttacgt cctcgccggc ggaccggcgg
     4801 acaaggccgg cctgcgcgcc ggcgaccgca ttctttccgt caacggcaat cccgtttccc
     4861 gcttcagcgg catgggcaac gtcgaggcca gcatcgtctg gaacatcgcg cgcagcgagg
     4921 aaccgtccct gccgatcgaa atcgagcggg atggcaaaac gcagaccgtc tgggtggagc
     4981 cgtccctgcc cacccggcag ggctggagcc gcaagaacct gcgccagatc gcgatcgccc
     5041 cggcgcagac gccgatgatc gccaaggtca tcccgggcag cccggcggcc gccgccgaac
     5101 ttcgcccgca ggatttgatt ctcgccatca acggccagaa actcctcagc ctgcccggcc
     5161 tcgccgaata cctcaaccaa tacagcggcg gaccggtgac tctcacggtc agtcgggatg
     5221 gccagacctt cgaacagccg gtgactccgc agattcccgt ggacggcgac aagccgcgga
     5281 tcgggattca atgggacgac cgcggcttga cgacgctttc gcatccgaac cccatcgcgc
     5341 agatcgtcgc cagcgccgag accatgtggg ccacgctgac ggccatcgcc tcgccgaaat
     5401 cggacatcaa gatcgagcac ctcagcggcc cggtgggcat catgcggctc tattacctca
     5461 tctttgaagc gccggacggc tggcgcgtcg cgctctggtt cagcgtcatt ttcaacgtga
     5521 acctcgccat tctcaacctc ctgcccctgc cggtgctgga tggcggccac atcacgctct
     5581 cgctcatcga gctcatccgc cgccgcccgc tggacatccg gctc
//
LOCUS       DATDAY010000024         3484 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_259945, whole genome shotgun sequence.
ACCESSION   DATDAY010000024 DATDAY010000000
VERSION     DATDAY010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 3484)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3484)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3484
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_259945"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..215
                     /locus_tag="VIS74_00510"
     CDS             <1..215
                     /locus_tag="VIS74_00510"
                     /inference="COORDINATES: protein motif:HMM:NF019303.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEY5791746.1"
                     /translation="RTKGQDIFRMKGVFAVKGTKDRVVFQGVHMLLDAATGPAWGNAA
                     RQNRLVFIGRNLDRDELASGFQKCLL"
     gene            220..1224
                     /locus_tag="VIS74_00515"
     CDS             220..1224
                     /locus_tag="VIS74_00515"
                     /inference="COORDINATES: protein motif:HMM:NF024298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5791747.1"
                     /translation="MPLTKISEISAEDHLLDAAWSPDGARLALTPSHGPVLITDTSGN
                     LQRELPGHGLSNGVGSWNGDQFATCGSDGQLRIHDLRASAPPRDIALGKNWIEHAKWS
                     PDGRYLAAGAGRSLIILDAAGEKIAEFSGHKSTVCDFAWNPRNPREIVSVCDGGAHMW
                     RIGETEPYARFDWGGASLLALWSPDGRWAVTGDQTPSVHLFDFTRDHPLHIQGYETKV
                     RALSFNAGSTRLASGGGPLVTVWNCTGEAGPEGSVPKQLQGHAKDALALAYHPAADLL
                     ASGGQDGLLILFKPETRTTAAGLEKLKSAVTAVAWNPAGNRLAAGTEDGVLHLFAHTP
                     "
     gene            1228..1659
                     /locus_tag="VIS74_00520"
     CDS             1228..1659
                     /locus_tag="VIS74_00520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5791748.1"
                     /translation="MIPAILTVLGLGFVYFISAIPAGVALKLHPGLAALVAWLGYSLG
                     SWVILLVGAPLRNWVAKRVKLLNEPRDDSKLFWRIWRRFGLPGLGLIAPVTCGPQGGS
                     VIALALGEKPARIGFWFSVGGIPYAIGFALAAAFGKHLASG"
     gene            1840..1911
                     /locus_tag="VIS74_00525"
     tRNA            1840..1911
                     /locus_tag="VIS74_00525"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1872..1874,aa:Lys,seq:ttt)
     gene            complement(1916..2692)
                     /locus_tag="VIS74_00530"
     CDS             complement(1916..2692)
                     /locus_tag="VIS74_00530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5791749.1"
                     /translation="MTLGLLAGAGLLHLLPRLGPGGRRLSEACCRAPLLDAIVAYFTV
                     LPLIAGPLCAGWTGLFLAILAQLTTVLVWQTLHEAVHRDAVKGPRIIKVLNKKFGAFR
                     NLTAVYVTALATPIFWFVRMGELIVYPPLVWLVHLPPYKAADWINVSRQKFSGLVGHD
                     LIWCLYCDWMTGVWSLGTEMLRNVESFWCPIRFSCEKKCENCAIDFPDVNNGWVPATK
                     GMAEVAETLDRMYSSPKEPQPWFGHPLRKEKPADSGEDPV"
BASE COUNT          680 a         1135 c         1061 g          608 t
ORIGIN      
        1 tgcgcaccaa gggccaggac attttccgca tgaagggggt cttcgccgtg aagggcacga
       61 aggatcgcgt cgttttccag ggcgttcaca tgctgctcga cgcggccacc ggccccgcct
      121 ggggcaatgc cgcccgccag aaccggctcg tgttcatcgg gcgcaatctc gaccgggacg
      181 aactcgcctc cggcttccaa aagtgcctgc tttaacctca tgccgctgac gaaaatttcc
      241 gaaatttccg ccgaggacca cctgctcgac gccgcctggt cgccggacgg cgcccgcctc
      301 gcgctcacgc cgagccacgg ccccgtcctc atcaccgaca cctccggcaa cctccagcgc
      361 gagcttcccg gccacggctt gagcaacggc gtcggctcct ggaacggcga tcaattcgcc
      421 acttgtggca gcgacggcca gctccgcatc cacgatctcc gcgcctccgc gcccccgcgt
      481 gacatcgcct tggggaaaaa ctggatcgag cacgccaagt ggtcgccgga tggccgttat
      541 ctcgccgccg gagcgggccg atcattgatc attctcgacg ccgcgggcga aaaaatcgcc
      601 gaattcagcg ggcataaaag caccgtctgc gacttcgcct ggaatccccg caatccccgc
      661 gagattgtct ccgtctgcga cggcggcgcc cacatgtggc gcatcgggga aaccgaaccc
      721 tacgcccgct tcgactgggg cggcgcgagc ctgctcgccc tttggagccc cgacggccgc
      781 tgggcggtga ccggcgatca gacccccagc gtgcacctct tcgacttcac ccgcgatcac
      841 ccgctgcaca tccagggcta cgaaaccaag gtccgggcgc tcagtttcaa cgccggctcc
      901 acccggctgg ccagcggcgg cggaccgctg gtcacggtct ggaattgcac cggcgaagcc
      961 ggcccggaag gttcggtccc caagcagctc cagggccacg cgaaggacgc cctcgcgctg
     1021 gcttatcacc ccgccgccga tctgctcgcc tccggcgggc aggacggatt gctcatcctt
     1081 ttcaaacccg aaacgcggac aaccgccgcg gggctcgaaa aattaaaatc cgccgtcacg
     1141 gccgtggcct ggaatcccgc cggcaaccgc ctcgccgccg gaacggagga tggcgtcctg
     1201 catctgttcg cccatactcc ctgacgcatg attcctgcca ttttgaccgt gctcggcctc
     1261 ggctttgtgt attttatcag cgcgattccc gccggggtgg ccctgaagct gcacccgggc
     1321 ctcgccgccc tggtcgcgtg gctcggttat tcgctcggct cctgggtgat tctgctcgtc
     1381 ggcgccccgc tgcgcaattg ggtggcaaag cgggtcaaat tgctcaacga accgcgcgat
     1441 gactccaagc tgttctggag aatctggagg cgcttcggcc tgcccggcct cggattgatc
     1501 gcccccgtca cctgcggccc gcaggggggc tccgttatcg cgctggccct cggcgaaaaa
     1561 cccgcgcgga tcggcttctg gttcagcgtg ggcggaattc cctacgccat cggcttcgcg
     1621 ctggccgcgg cgttcggaaa acacctcgcg tccgggtaga ggccctccaa atttgaacgc
     1681 tgcgaactcc gcgttctccg cggtcaaaaa atgaccggcg cgcatttttc caaaatcggg
     1741 gcttgcgatc cgcccggaaa tcccgcttta tttccggccc gcgaaagcgc gcttcgggtt
     1801 gatcttcgcc cggaagtgtg cgaaaaaagc gggactcatg ggttggtagc tcaatggtag
     1861 agcagtaccc ttttaaggta ttggttctgg gttcgagtcc cagccaaccc actttttaaa
     1921 ccggatcttc gccggaatcg gccggttttt cctttctgag cggatggccg aaccacggct
     1981 gcggctcctt cggcgaggaa tacatgcggt ccagcgtctc ggcgacctcc gccatgcctt
     2041 tcgtagcggg cacccagcca ttattgacgt cggggaagtc gatcgcgcaa ttctcgcatt
     2101 ttttctcgca ggaaaagcgg atcgggcacc agaacgactc cacgttccgc agcatctcgg
     2161 tcccaaggga ccagacgccg gtcatccagt cgcaatacag gcaccagatg agatcgtgcc
     2221 cgaccaggcc ggaaaacttc tggcgcgaca cattgatcca atccgccgcc ttgtaaggag
     2281 gcagatggac cagccagacc agggggggat acacgatcaa ttcgcccatg cggacaaacc
     2341 agaaaatggg cgtcgccagc gccgtgacat agaccgccgt gagattgcgg aaagcgccga
     2401 attttttgtt gagcacctta atgatccggg ggcccttcac cgcgtcccga tgaacggcct
     2461 catgaagggt ctgccagacg agaacggtgg tgagttgcgc gagaatggcg aggaacagcc
     2521 ccgtccaccc ggcacaaagc ggaccggcga tcaaaggaag gaccgtgaag taagcgacaa
     2581 tcgcatccag gagcggcgcc cggcagcagg cttcggagag acgcctcccc cccggcccga
     2641 gccgcggaag aagatgaagc agccccgcgc ccgccaggag gcccaatgtc accgtcgcat
     2701 agaatgccag atagccacac atgagggcag cagtaaatcc gccggccggg gaaaagccag
     2761 ctgaaaaggg agcttggctc ttcaaagcgg cggtttaccg gttgaaggca agttggatcg
     2821 tttcgttgca aggagccggg gcgggatgcg ccgagaagga cttcacatgg ctcccgcaca
     2881 cggtcaggac ggcttccatg aagggatgca actgggtggg ttgtcgaagg ggcagccttc
     2941 tccggggaag attcgccggc cttctcggga aaggcatggg agcagttgcg gatgatctgc
     3001 ggatgttccg cataccgctg gcatccgcag cagatcaaaa aatgcacgcc aaggagcagg
     3061 cgcgtcccga gcggcagcct gcggtccatt ccctgggata aataaaatgc gcacaacctc
     3121 ggggcgtttc ggcgtgtggc gcccgatgaa cttcacgagc tgcgtttcca gccagccgcg
     3181 ccgtttgagc agggagggat tcccgtgagc gcggggccgc ccttgcggga cggcgaatcg
     3241 ggaggcgggt cggaagattc caaaaagggg ccggacaacg ggaacggggc ctccgccgag
     3301 accgccagca aagcaatgtt ttttaaaaaa attttgacat aaaccaatat taaagttaat
     3361 ataactaaca tcaatatgac catcgatgcc ccgccttctt cgcccaagcg tgtttaccgc
     3421 cagtcgctgc gggccgaggc ggccggccgg acgcaccggc ggatcgccga gtgtttcctg
     3481 ggcc
//
LOCUS       DATDAY010000025         2691 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_568742, whole genome shotgun sequence.
ACCESSION   DATDAY010000025 DATDAY010000000
VERSION     DATDAY010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2691)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2691)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2691
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_568742"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..214)
                     /locus_tag="VIS74_00535"
     CDS             complement(<1..214)
                     /locus_tag="VIS74_00535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5791750.1"
                     /translation="MSLAVQIAEIESLTPRVKRFRLRGVRGALPSYSPGSSLRIHLPV
                     GSGLWNAYSLTSDPWADGDYAIAVRRE"
     gene            complement(211..807)
                     /locus_tag="VIS74_00540"
     CDS             complement(211..807)
                     /locus_tag="VIS74_00540"
                     /inference="COORDINATES: protein motif:HMM:NF041259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylamine monooxygenase subunit DmmA family
                     protein"
                     /protein_id="HEY5791751.1"
                     /translation="MGSIKSQPVYRSLSRVPGAKHHVFASAGEGAISVKQVLEDLALN
                     ELSLAVLHWEIPATGNRYAALLENEFGAPLEVFPTEAGLMGALAEKLAQSPAGTVLYL
                     SGPEQFIWKAAALGRSAGMSRAQMQAELRGSRARRVYCVHCKAINEGVTHSIHRCAGC
                     GLPLCVRDHYSRLMEAYAGFRIDAEEPGNVPEAEELYA"
     gene            complement(821..1270)
                     /locus_tag="VIS74_00545"
     CDS             complement(821..1270)
                     /locus_tag="VIS74_00545"
                     /inference="COORDINATES: protein motif:HMM:NF018008.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5791752.1"
                     /translation="MSEYLADQPGIKLLKYGTAELVDLEKSYQCPGLISRILDFPVTD
                     QKAGLTVGRFEMRPSVDFPFYYEYLEVKTIVTGKIVVRDEDGVKYVAEPGDVYIFSPP
                     TLVMFCAESDGTAVYLGHRLKEPSFLPGFPGLVETPIPDKVKAWWTR"
     gene            1525..>2691
                     /locus_tag="VIS74_00550"
     CDS             1525..>2691
                     /locus_tag="VIS74_00550"
                     /inference="COORDINATES: protein motif:HMM:NF013104.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5791753.1"
                     /translation="MEPDQFTELLRGDATLKYLGVFASQAFYYICAVLMLLIHVGFLA
                     YEGGAARTKNVLATMMKNLMTVSVVGLGWYLIGWYIYNGFPLWPVGPALGPWTNPDTL
                     SDALKPVFALTQASYPWSEALTPTKADEMTGVFCFAFALFAMTTASIMSGALIERIHV
                     GAYYILSFILGTFVWTFAAAWGWNFFGWFTTKLGYHDFGCSMVVHGIAGCFTLGVLIN
                     LGPRIGKYVDGKPRPILPHNLGLTMIGLMLIYTGFFFFLSCCVIFLGGYAGITSIYGT
                     PTTLSSIAMNTMMALAAGFAGAYFGSKGDPFFTASGGLAGIIAVSAGMDLYHPGIVAV
                     VAFIVASIMPKVALFIEKIGIDDAVGAFAVHGFCGIIGALLPGILALLESLKPRA"
BASE COUNT          503 a          895 c          743 g          550 t
ORIGIN      
        1 cctcccggcg aacggcgatc gcgtaatccc cgtccgccca cgggtccgaa gtcagcgaat
       61 aggcattcca caagcccgaa cccaccggca gatgaatccg caggctgctg cccggcgagt
      121 aggacggcag cgcgccccgc actccgcgca ggcggaaacg tttcacccgc ggggtgagcg
      181 attcgatttc ggcaatttga acggccaggc tcatgcgtaa agctcctccg cctccggcac
      241 gttgcccggc tcctccgcgt caatccgaaa gcccgcgtag gcttccatca agcgcgaata
      301 atgatcccgc acgcacagcg gcaatccgca gcccgcgcag cggtgaatgg agtgcgtgac
      361 cccctcgttg atcgccttgc aatgcacgca atacacccgc cgcgcccgcg agccgcgcag
      421 ctcggcctgc atttgcgcgc ggctcatgcc cgcgctccgg cccaaggcgg cggccttcca
      481 gatgaattgc tccggaccgg aaagatacag caccgttccc gccgggcttt gcgccaattt
      541 ttcagcgaga gcgcccatca gcccggcttc ggtgggaaaa acctccagcg gcgccccgaa
      601 ttcattttcg agcagcgccg cgtagcgatt gccggttgcc ggaatctccc aatgcagcac
      661 ggccagcgaa agctcgttca gagcgagatc ctccagcacc tgcttcaccg aaatcgcgcc
      721 ctcccccgcc gaggcgaaca catgatgctt cgccccggga acccgggaga gcgaacggta
      781 aaccggctgg ctcttaatgc ttcccatggg agcgcccccc ttatcgcgtc caccaagcct
      841 tgaccttgtc gggaatcggc gtttccacca gccccggaaa gccgggcagg aacgacggct
      901 ctttgagccg gtggcccagg taaaccgccg tgccgtcgct ctccgcgcag aacatcacca
      961 gcgtcggcgg cgaaaaaata tacacatccc ccggctcggc cacgtatttc accccgtcct
     1021 cgtcgcggac gacgatcttt ccggtcacga tcgttttgac ttcgaggtat tcgtaataga
     1081 acggaaaatc caccgagggc cgcatctcga aacgccccac ggtcagcccg gctttttgat
     1141 cggtgaccgg aaaatccagg atgcggctga tcagccccgg acattgatag gatttttcca
     1201 ggtccacgag ttcagcggtt ccgtatttga gcaatttgat tcccggttgg tcggcaaggt
     1261 attcagacat agcgatgggt tgtttctttt tatacaactt gtgtttccca gcagaatcca
     1321 tgccgctctg gggaggaaaa tcgcaaaaaa acaaaaaagt ttctccataa gcaaccttag
     1381 tttttcacat tggcacgcga agtgctttcg gattttccga atccaaggac gcggcgttcc
     1441 atgtcctcga tgactcgagg ccatccctgc cattccacgt cgttgcattc accttaatca
     1501 actcaaccaa gaaaaaacag gactatggaa ccggatcaat tcacggagct cctccgggga
     1561 gacgctacct taaagtatct gggcgtattc gcctcgcagg cgttttatta catctgcgcg
     1621 gtgttgatgc tgctcatcca cgtcggcttc ctcgcctacg aaggcggggc ggcgcgcacg
     1681 aagaacgtgc tggccacaat gatgaaaaat ctcatgaccg tgtcggtggt cggtctggga
     1741 tggtatttga tcggctggta tatctacaat ggctttccgc tctggccggt gggaccggcg
     1801 ctggggccat ggaccaaccc cgacaccctg agcgacgccc tgaagccggt cttcgccctc
     1861 acccaggcct cgtatccctg gtcggaggcg ctgacgccca cgaaagccga cgaaatgact
     1921 ggcgtcttct gcttcgcctt cgcgctgttt gcgatgacga cggcctccat catgtccggc
     1981 gcgctcattg aacgcatcca cgtgggcgcc tactacatcc tgagcttcat cctcggcacc
     2041 ttcgtctgga ccttcgccgc cgcctggggc tggaacttct tcggctggtt caccacgaag
     2101 ctgggttacc acgacttcgg ctgttcgatg gtcgtgcacg gcatcgccgg ctgcttcacg
     2161 ctcggcgttc tgatcaatct tgggccgcgc atcggcaaat acgtggacgg caagccgcgc
     2221 ccgatcctgc cgcacaacct cggccttacc atgatcggcc tcatgctcat ctacaccggg
     2281 ttcttcttct tcctttcctg ctgcgtgatc ttcctcggcg gctacgcggg aatcacctcc
     2341 atctacggca cgccgaccac gctgtcctcg atcgcgatga ataccatgat ggcgctcgcc
     2401 gcgggatttg cgggagccta cttcgggtcc aagggcgacc cgttcttcac cgcctcgggc
     2461 ggtctggcgg gcatcatcgc cgtcagcgcc ggcatggatc tctaccatcc gggcatcgtg
     2521 gcggttgtcg cctttatcgt ggcgtccatc atgcccaagg ttgcgctctt cattgagaag
     2581 atcggcatcg acgacgccgt gggcgcgttc gccgtgcacg gcttctgcgg catcatcggc
     2641 gcgctgctgc cgggcatcct cgcgctcctc gaatcgctca agccgcgcgc g
//
LOCUS       DATDAY010000026         3503 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_1689632, whole genome shotgun sequence.
ACCESSION   DATDAY010000026 DATDAY010000000
VERSION     DATDAY010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 3503)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3503)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3503
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1689632"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..546)
                     /locus_tag="VIS74_00555"
     CDS             complement(<1..546)
                     /locus_tag="VIS74_00555"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEY5791754.1"
                     /translation="MKTAFVTGSTGLLGNNLTRTLAERGMRVKALARSPEKARRQFGV
                     LKNVEIVPGDLTEIGGFAGALGGSDVLFHTAAFFRDNYKGGNHRRTLAAVNETGTARL
                     LDAAHAAGIRRVVHASSIAVLMGRGGRPADETMLRPEHEADDYYLSKIRSEKVVLEFL
                     DRHPELTATLVLPGWMHGPGDM"
     gene            complement(543..680)
                     /locus_tag="VIS74_00560"
     CDS             complement(543..680)
                     /locus_tag="VIS74_00560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5791755.1"
                     /translation="MILASLLLILRFLAGPARRLIARRCPAAMAWLNPAGRVSGTKER
                     P"
     gene            736..1401
                     /locus_tag="VIS74_00565"
     CDS             736..1401
                     /locus_tag="VIS74_00565"
                     /inference="COORDINATES: protein motif:HMM:NF025343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEY5791756.1"
                     /translation="MVFSEKMKSNASAPPRRKRLSREESRAETRRQLLDAAWGLFAEK
                     GFEGCSVDELVERAGYTKGAFYSNFESKDAIFLELLKQHKAETLGRLKALLADGCGME
                     ELLRRLEAYNRDLEKDTVWCLLSVEFQVRAAREPAFRAVFEELSRADRAKVAVFIKTL
                     FQKAGAKLPARAEHLAVAFMALIQGLTLRSIAEPRALPAGTTGKIVSLFQRSLLDRNG
                     GSE"
     gene            complement(1512..1646)
                     /locus_tag="VIS74_00570"
     CDS             complement(1512..1646)
                     /locus_tag="VIS74_00570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5791757.1"
                     /translation="MAVVVFALNAINVPPADFANRSLQSAANSRKNTGGRLYMTFGQY
                     "
     gene            1645..2790
                     /locus_tag="VIS74_00575"
     CDS             1645..2790
                     /locus_tag="VIS74_00575"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY5791758.1"
                     /translation="MNTSTTTRRRFLSTAAMSAAAAAAAPLLSQAAAAGGLSAAAPSD
                     QGLPTNPPSPTGHYRPPYRFGLGGVAIGNGFRPATDAEAGETLAAAWAGGVRYYDTSP
                     WYGLGLSERRFGHFLHNQNRGDYVLSTKVGRVLSPSQPLGNLLWKNPAPFKHTYDYTA
                     AGARRSIEDSLQRLGVERIDIVFIHDLSPDNPDLGERWPEYFDIAAKGAMPELTKMRE
                     EGLIKAWGFGVNTIEPILRALNEGDPDIFLSATQYSLMHHEEEIARVFPACEKRGVSL
                     VVGAPLNAGFLAGADRYDYRGKMPEGFLAKRDRLSALAKEYGTDLRTAALQFCAAPAV
                     VSSVIPGARSARQVTENIASMKVKIPAAFWAELKREKLIAENAPTPA"
     gene            complement(2792..>3503)
                     /locus_tag="VIS74_00580"
     CDS             complement(2792..>3503)
                     /locus_tag="VIS74_00580"
                     /inference="COORDINATES: protein motif:HMM:NF014376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HEY5791759.1"
                     /translation="GSGKMGGMDSKPDVTRTNLPSRKGSIPMPDRRAFLRAGIGIALG
                     LGGIWKTLAQSNEGGPVPPEATVFADDGTTPNSRFAVLVYRQAFSPSTPDLSEAIAAR
                     FAANGWTGTWRYPGIFTFNHYHSTTHEVLAIYQGSGTLQLGGLKRGRPFDVASGDVIL
                     VPAGVGHMQVKGSADFRVLGAFAGGRSWDLNRGLPGERPQADRHIAAVPLPDNDPLFG
                     ADGPLVKIWKSADTRNDS"
BASE COUNT          621 a         1138 c         1133 g          611 t
ORIGIN      
        1 catgtcgccg gggccgtgca tccaccccgg cagcaccagc gtggcggtca gctccggatg
       61 gcggtcgaga aattccagca ccaccttctc gctgcggatc ttgctgagat aataatcatc
      121 cgcctcgtgc tccgggcgga gcatcgtttc gtccgccggc cggccgccgc ggcccatgag
      181 cacggcgatg gagctggcat ggaccacgcg ccggatcccg gcggcgtggg cggcgtcgag
      241 caaccgggcc gtgcccgtct cgttcaccgc cgccagcgtc cggcggtggt ttccgccctt
      301 gtagttgtcg cgaaagaacg ccgcggtgtg gaaaagcaca tcgctgccgc cgagcgctcc
      361 ggcaaaaccg ccaatctccg tcagatcccc cggaacgatc tcgacgtttt tcaggacgcc
      421 aaactggcgc cgggcttttt ccggcgagcg cgcgagggct ttcacccgca tgccccgctc
      481 cgcgagcgtt cgcgtcagat tgtttccgag cagtccggtg gagccggtga caaaggcggt
      541 tttcatgggc gttccttcgt tccggaaacc cggccggccg ggttgagcca ggccattgcc
      601 gccgggcagc ggcgggcaat caggcgccgc gccgggccgg ccagaaaccg caggatcaga
      661 agcagggatg cgaggatcat tgtgaattta aaataccaat tagtttttaa atactaattg
      721 gttttcaaaa atcaaatggt attttctgaa aagatgaagt cgaatgcatc cgcgccgccc
      781 cggcgcaagc gcctgtcccg ggaggagagc cgggcggaga cccgccgcca gctgctggac
      841 gccgcctggg ggctgttcgc ggaaaagggg tttgaaggct gctccgtgga cgaattggtg
      901 gaaagggcgg gttacaccaa gggcgctttt tattccaatt tcgagagcaa ggacgccatt
      961 tttctcgaac tgctcaagca gcacaaggcg gagaccctcg gccggctgaa ggccctcctt
     1021 gccgacggct gcggcatgga ggaactgctg cggcggctgg aggcctacaa tcgggatttg
     1081 gagaaagaca cggtgtggtg cctgctttcc gtcgagtttc aggtgcgggc cgcgcgcgaa
     1141 ccggcgttcc gcgccgtgtt cgaggaattg tcccgcgccg accgggcgaa ggtggcggtc
     1201 ttcatcaaaa ccctcttcca aaaggcggga gccaagcttc cggcccgggc ggaacacctc
     1261 gccgtggctt tcatggcctt gattcagggc ctgaccctgc ggagcatcgc cgagccgcgc
     1321 gcgctgcccg ccggaaccac cgggaaaatc gtcagcctgt tccagcgttc cttgctggat
     1381 cggaacggcg gttccgaata gaggtccttc gattccgttt gccgccgtga gcggcggcgt
     1441 tggcaacgct gtcccaccgg gagatcggtt cgcggcaggg aggggttgcc aaccccgccg
     1501 ggggaatgcg ttcaatattg gccgaaggtc atgtaaagcc ggccgccggt gtttttccgg
     1561 ctattcgcgg cactttgcag ggaacggttt gcgaagtccg ccggcggcac gttgattgca
     1621 ttcaacgcaa aaacgaccac cgccatgaat acctcgacca ccacccgacg ccgtttcctt
     1681 tccaccgccg cgatgagcgc cgccgccgcg gcggccgcac ccttgctttc gcaagcggcc
     1741 gcggcgggcg ggctgtccgc cgcggcccct tcggatcagg gactgccgac caacccgcct
     1801 tcccccaccg ggcactaccg tccgccctac cggttcggcc tgggcggcgt ggcgatcggc
     1861 aacggcttcc ggcccgccac cgacgccgag gccggggaaa cgctggcggc cgcctgggcg
     1921 ggaggcgtgc gttattacga cacctcgccg tggtatgggt tgggcctgag cgaacggcgc
     1981 ttcgggcatt tcctgcacaa tcaaaaccgc ggcgactacg tgctctcgac caaggtcggg
     2041 cgcgtcctga gccccagcca gccgcttggc aatcttttgt ggaagaatcc cgcgcccttc
     2101 aaacacacct acgattacac ggccgccggc gcgcgccgct cgatcgagga cagcctgcag
     2161 cgtttgggcg tcgagaggat tgacatcgtg ttcatccacg acctttcgcc ggacaatccc
     2221 gacctgggcg aacgctggcc cgagtatttc gacatcgccg cgaagggcgc gatgccggag
     2281 ctgacgaaga tgcgcgagga gggcttgatc aaagcgtggg gcttcggcgt caataccatc
     2341 gaaccaattc tgcgggcgct gaacgagggt gatcccgaca tctttctctc cgcgacccaa
     2401 tattcgctga tgcatcacga ggaggaaatt gcccgggtct ttccggcgtg cgaaaagcgg
     2461 ggggtgtccc tcgtggtggg tgcgccgctc aacgcgggct tcctcgccgg agcggaccgc
     2521 tacgattacc gcggcaagat gccggaaggt ttcctggcca agcgcgaccg cctcagcgcc
     2581 ctcgcgaagg aatacggcac cgacctgcgc acggccgcgt tgcagttctg cgccgcgccg
     2641 gccgtcgtgt cgtcggtgat tcccggcgcg cgttcggcgc ggcaggtgac ggaaaacatc
     2701 gcctccatga aggtgaaaat ccccgccgcg ttctgggcgg aattgaagcg cgaaaagttg
     2761 atcgcggaaa acgcgccgac gcccgcctga cttacgaatc gtttcgagtg tccgcagatt
     2821 tccaaatctt caccagcggc ccgtccgccc cgaaaagcgg atcattgtcc ggcagcggca
     2881 cggcggcgat atggcgatcc gcctgggggc gctcgcccgg aagtccgcgg ttcagatccc
     2941 aggagcgccc gccggcaaac gccccgagca cccggaagtc ggcgctgccc ttcacctgca
     3001 tgtgcccgac gccggcggga accaaaatca catcgccgct cgccacgtcg aacgggcggc
     3061 ctcgcttcaa gccgccgagc tggagcgtgc ccgaaccctg atagatcgcc agcacctcgt
     3121 gcgtggtgct gtggtagtgg ttgaaggtga agatgccggg ataacgccac gttccggtcc
     3181 agccattcgc ggcaaaacgc gccgcgatcg cctcggaaag gtccggcgtg gatggagaaa
     3241 aggcctgccg gtaaaccaga acggcaaacc ggctgttggg agtagttccg tcgtctgcga
     3301 agaccgtggc ttcgggcggg accggcccgc cttcgttcga ttgggccaga gttttccaga
     3361 tgccgcccaa ccccagcgcg atcccaattc ccgcgcggag aaacgcccgg cggtccggca
     3421 tcgggatcga acccttccgg ctgggaagat tggtgcgagt gacatcgggt ttcgaatcca
     3481 ttccccccat tttccccgaa ccc
//
LOCUS       DATDAY010000027         7092 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_1146447, whole genome shotgun sequence.
ACCESSION   DATDAY010000027 DATDAY010000000
VERSION     DATDAY010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 7092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7092)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7092
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1146447"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(483..1574)
                     /gene="sucB"
                     /locus_tag="VIS74_00585"
     CDS             complement(483..1574)
                     /gene="sucB"
                     /locus_tag="VIS74_00585"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0006554 - lysine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HEY5791760.1"
                     /translation="MSFEVKVPAVGESITTGVISAWHKKDGESITSGEVLFTLETDKI
                     STEVTAQESGIVKLQAAEGEEVKIGQTVAVIEAAGAAPAPKAEEKPPVPEAAPAPAPE
                     SAPVLAPAPVAEAPKPEPPAPKAATVPDDARTTRKRLTPLRRKIATQLVAAQQTAAIL
                     TTFNECDMSAVMQLRKELQEEFQKKHGVKLGFMSFFIKAAVDALKAVPGINSRLDGDE
                     LVTNHYHDIGVAVGTEKGLIVPVLRDCDAKSFAELEGDLGAYAVKAREGKIGIEDLQG
                     GVFTISNGGVYGSLLSTPILNPPQSGILGLHKIQERPMAVKGQVVIRPMMYLALSYDH
                     RVVDGKEAVTFLVRVKDCIENPARLLLGA"
     gene            complement(1625..4390)
                     /locus_tag="VIS74_00590"
     CDS             complement(1625..4390)
                     /locus_tag="VIS74_00590"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HEY5791761.1"
                     /translation="MKSSFTARWNEALLEDNYERWQSNPGEVDQNWAAFFEGFELGMA
                     RLKKNGAAIDANGAADTAAGGDPQWQTKVEALVYAYRTLGHSIAKLDPLAQKRPEQPL
                     LTLKELGFSESDLDKTVSSRFFRDNQRMKLRDMIAELKRLYSGTIGAEFMHIQNPRQR
                     SWVLSRMESRSEAARTDANAHRDLLRKIFEAETFESFIHTRYVGQKRFSLEGGEALIV
                     ALNAVLQRCPTHGVQEIVMGMAHRGRLNVLANFLNKPLSVIFHEFKGNYVPGSVGGNG
                     DVKYHLGYQTTRDLANGYQVEIRMAANPSHLEAVDPIVQGKARARQRVLNDTENRKKV
                     VPLLLHGDAAFIGQGVVAETFNMSQLPGYRTGGTVHIVVNNQIGFTTLPADSRSTPYC
                     TDIAKMIDAPIFHVNGEDPVAVVQVAEMALEFRQEFGRDVVIDIYCYRRHGHNEGDEP
                     NFTQPDLYNKIKAHSLLSDIFIKETEQAGTVSRAEAEILKSESLQELEAALAAVKADE
                     EAESKKSEFTGSTAIFQPPYSHEPANTAIGAETLEKIVNALTTVPDSFRVLPKVRRIL
                     LDRRQQVWKSGGPYNWAYAEALAFGSLLLEGTPVRLSGQDSRRGTFSHRHSVIYDEVS
                     RERHIPLNHLDPAQARFCVYNSPLSEYAVLGFDYGYSMDFPSMLCLWEAQFGDFTNGA
                     QIIIDQFIASAESKWQRPSSIVLLLPHGYEGQGPEHSSARLERFLQLCAEDNMQVCNL
                     TTPAQYFHVLRRQIHRGYRKPLIIMTPKSLLNAELATSKTEDFTNSRFYEILEGPAPA
                     NPKKVDHVILSSGKVYYDLLQYREKNGLNDTSALLRLEQIYPLYDEKLIRLATPYLSK
                     NTKIVWCQEEPRNMGAWCFVKPKLERALGREIHYAGRPESASTAAGTLKVHQRQQEEL
                     VKQAFSL"
     gene            complement(4494..4877)
                     /locus_tag="VIS74_00595"
     CDS             complement(4494..4877)
                     /locus_tag="VIS74_00595"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEY5791762.1"
                     /translation="MTFDTCFLIDLQRERRRGEAGAACRFAEAHAGQTSYLSAVAWGE
                     FSEGFAAADAVFLAWQASFEILPLDGRVAWQYSRLVRELRARGQLIGSNDLWICATAL
                     AHHLPLVTRNAADFQRIPGLKVLGY"
     gene            complement(4874..5125)
                     /locus_tag="VIS74_00600"
     CDS             complement(4874..5125)
                     /locus_tag="VIS74_00600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin VapB family protein"
                     /protein_id="HEY5791763.1"
                     /translation="MATKTISIDLEAYDRLAGARLTPGESFSRVIKRAEWQSAPRTLG
                     RLLNLVRETEPLPESVLDRLEAAQREDAPPDDPGNHPTP"
     gene            5251..6183
                     /locus_tag="VIS74_00605"
     CDS             5251..6183
                     /locus_tag="VIS74_00605"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY5791764.1"
                     /translation="MIEVENLTKRYSGAAALDGISFQVGKGEIVGFLGPNGAGKSTTM
                     RILSGFMPPSSGRASVAGFDVCRESLRAREHVGYMPENVPLYTEMRVGEYLRYRAALK
                     GVRGRKVPERVNDVLELCGLADVARKLIGKLSKGYRQRVGLADAMVHEPDLLILDEPT
                     IGLDPNQIRQVRDLIRNLARHHTILLSSHILSEVELTCHRVIILNRGRIEALDTPQNL
                     RQRLRSGGEIRLEIHAANSGSALPELKKIPGVQSARFDSEGDWHLFHLQTEAGADPRE
                     AIFAHCVSRGWKVRELTLTRTSLEDVFVEMTQPE"
     gene            6187..6945
                     /locus_tag="VIS74_00610"
     CDS             6187..6945
                     /locus_tag="VIS74_00610"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY5791765.1"
                     /translation="MRKLSILFGRELAHYFRSPLACTVLCFFLVLTGFNFQYGVSSLN
                     LQPLTTTIVEAYFNTVFFWYPFVLVFPLITMRTFSEEYKLGTIEPLMTAPVRDWQVVM
                     AKYLGALVFYLILWLPSLLYFVIFQNTTGFRAAEAVGSYFGAYSMLLLIGMFYIALGC
                     LASALTENQIVAAIISFCLTCVMFFMSLISFLTPQISPVLRDLSSYLSPIEHMAGFSQ
                     GIFDTRPVVYYVSLTVFTLYLTLQIFQSRRWKTA"
BASE COUNT         1243 a         2181 c         2178 g         1490 t
ORIGIN      
        1 tcaaacgttt gtttgaaacg aatgtttcat ccattgccgg attccgcaat gcaaattttg
       61 gaccactaaa aaaacggccg ggccggccgt tttgtgattc tatagtattt cgcaaaagag
      121 atttccgaaa tacccaacgg gttgaggcgc ttgtcgggtg gggaaatggg gccccgccac
      181 cccattgggc gcctttggtt gcggggaaac ggggaccccg tttccccgtt tacgctgggc
      241 ggttggcaca attgccgcac ttcgtgcgcc cctcgggctg tcccgcttcc gcgggacagg
      301 cggtcttcgc tggctgcccc tgcattgaat cccttttcgg aaatctcttt tgagaatcgc
      361 tctaagtcat ggacggctcg gcgagccgtc cctacccaat caagacaggg cgggttcgag
      421 atagaagggg gcgggggcac cttcgcccca cccgccgccg tcgcttgttt tctcgggaaa
      481 aactacgccc ccaagagcag ccgcgccgga ttttcgatgc aatccttcac gcggacgagg
      541 aacgtcacgg cttccttgcc gtccaccacg cggtgatcgt agctcagcgc gagatacatc
      601 atcgggcgga taaccacctg gcctttgacg gccatcggac gctcctggat cttgtgcagg
      661 ccgagaatgc cgctctgcgg cgggttcagg atgggcgtgc tcagcagcga gccatacacc
      721 ccgccgttgg aaatggtaaa caccccgccc tgcaggtcct cgatgccgat cttgccctcg
      781 cgcgccttga cggcgtaagc gcccaggtcg ccttcgagtt cggcgaagct cttggcatcg
      841 caatcccgca ggaccggaac gatgagcccc ttttcggtgc cgacggcgac gccgatgtcg
      901 tgataatgat tcgtgaccag ctcatccccg tcgaggcggg aattgatgcc cggaaccgcc
      961 ttgagcgcgt ccaccgcggc cttgatgaaa aacgacataa agccgagctt cacgccgtgt
     1021 ttcttttgga attcctcctg cagctccttg cgcagctgca tgaccgcgct catgtcgcat
     1081 tcgttgaagg tcgtgagaat ggccgccgtc tgctgcgcgg cgacgagctg ggtcgcgatc
     1141 ttgcggcgca gcggcgtgag gcgcttgcgg gtcgtgcggg cgtcgtcggg aaccgtggcc
     1201 gccttcggcg cgggcggttc gggcttgggc gcttccgcca ccggagcggg cgccagcacc
     1261 ggcgccgatt caggagccgg ggcgggcgcg gcttccggca cgggcggttt ttcctcggct
     1321 ttcggcgcgg gggccgctcc agcggcctcg atcaccgcga cggtctggcc gatcttgact
     1381 tcctcgccct cggcggcctg gagcttgacg atcccggatt cctgggcggt cacctcggtg
     1441 gagattttgt cggtttccag cgtaaagaga acttcaccgg aagtgatgga ttcgccgtct
     1501 ttcttgtgcc aggcggagat gactccggtg gtgatggatt cgccgacggc ggggactttg
     1561 acttcaaaac tcatgctcgt gattgcgtaa aggacttaga ggactgactt aagaggctca
     1621 gggtttacag ggagaacgcc tgtttgacca gttcctcttg ctggcgctgg tgaactttca
     1681 gcgttcccgc cgccgtgctg gcgctttccg ggcgtcccgc gtaatgaatt tcgcggccca
     1741 gcgcgcgttc gagcttgggc ttgacgaagc accacgcgcc catgttccgc ggctcctcct
     1801 ggcaccagac gatcttcgtg ttcttcgaga gatacggcgt cgcgaggcgg atcaatttct
     1861 cgtcgtagag cggataaatc tgctccagcc gcagcagcgc ggaggtgtcg ttcagaccgt
     1921 ttttctcgcg gtattgcagc aggtcgtaat acaccttgcc ggaggaaagg atcacgtggt
     1981 ccaccttctt cggattggcc ggagcgggcc cctcgaggat ttcgtagaaa cgggagttcg
     2041 tgaaatcctc ggtcttcgac gtggccagct cagcgttgag caggctcttg ggcgtcatga
     2101 tgatgagcgg cttgcggtag ccgcgatgaa tctgccggcg caggacgtgg aaatactgcg
     2161 cgggggtcgt gagattgcag acctgcatgt tgtcctcggc gcagagctgg aggaagcgct
     2221 ccaggcgggc gctggagtgc tccggcccct gcccctcgta gccgtgcggc aggaggagca
     2281 cgattgagct cgggcgctgc cacttgctct cggcggaggc gatgaattgg tcgatgatga
     2341 tctgcgcccc gttggtgaag tcgccgaact gcgcttccca gaggcagagc atggacggaa
     2401 aatccatcga gtagccgtag tcgaaaccga gaacggcgta ttcggaaagc gggctgttat
     2461 acacgcagaa ccgcgcctgg gccggatcga gatgattcag cggaatgtgc cgctcgcgcg
     2521 aaacctcgtc gtaaatgacg gaatggcggt ggctgaacgt gccgcggcgg ctgtcctgcc
     2581 cgctcaggcg caccggcgtg ccctcaagca gcagcgaacc gaaggcgagc gcctcggcgt
     2641 aagcccaatt gtaagggccg ccggacttcc agacctgctg gcggcgatcc agcaaaatgc
     2701 ggcgcacctt cggcagcacg cggaagctgt ccggcacggt ggtcaaagca ttgacgattt
     2761 tttccagcgt ctcggcgccg atggctgtat tcgccggctc gtgggaatac ggcggctgga
     2821 agatggcggt cgaaccggta aattcggact ttttgctctc ggcttcctcg tccgccttca
     2881 ccgcggcgag cgcggcctcg agttcctgca gggactcgct cttgaggatt tccgcctcgg
     2941 cgcggctgac cgtcccggcc tgttcggtct ccttgatgaa gatgtcgctg agcagcgagt
     3001 gggccttgat cttgttgtaa agatcgggct gcgtgaagtt cggctcgtcg ccctcgttat
     3061 ggccgtggcg gcggtagcag taaatgtcga tgaccacatc gcgcccgaac tcctggcgga
     3121 attcgagggc catttcggcg acctgaacga cggccaccgg atcctcgccg ttgacgtgga
     3181 aaatcggggc gtcgatcatc ttcgcgatgt cggtgcaata cggcgtcgag cgcgaatcgg
     3241 cgggcagcgt ggtgaagccg atctggttat tgacgacgat gtggaccgtg ccgccggtgc
     3301 ggtaaccggg gagctgcgac atgttgaagg tctccgccac gacgccctgg ccgatgaacg
     3361 cggcgtcgcc gtgaaggaga agcggcacga ctttcttgcg attctccgtg tcgttgagca
     3421 cccgctggcg ggcgcgcgcc ttgccctgca cgatcgggtc caccgcctcg aggtggctcg
     3481 gattggcggc catgcggatt tcgacctggt agccgttggc gagatcgcgc gtggtctggt
     3541 agccgaggtg gtatttcacg tcgccgttgc cgcccacgga accgggaacg tagttgccct
     3601 tgaattcgtg gaagatgacg ctcagcggct tgttgaggaa attcgccagc acgttgaggc
     3661 ggccgcggtg ggccatgccc atcacaatct cctgcacgcc atgggtcgga cagcgctgga
     3721 gcacggcgtt gagcgcgacg atcagggcct cgccgccttc gagtgaaaag cgcttctggc
     3781 cgacgtaacg cgtgtggatg aagctctcaa acgtctcggc ttcgaaaatc ttgcggagca
     3841 gatcgcgatg cgcgttcgca tccgtgcggg cggcttcgga gcggctttcc atgcgggaga
     3901 gcacccagct ccgctggcgg ggattctgga tgtgcatgaa ctcggcgccg atcgtgccgg
     3961 aataaagccg cttcaattcg gcgatcatgt cgcgcagctt catccgctgg ttgtcgcgga
     4021 aaaaccggga ggaaaccgtc ttgtccaggt cgctctcgga aaatccgagc tccttcaagg
     4081 tgagcagcgg ctgctccgga cgtttctgcg ccagcggatc gagcttggca atggagtggc
     4141 ccagcgtgcg ataggcgtaa acgagggctt cgaccttcgt ctgccattgg ggatcgcccc
     4201 ctgccgcggt gtcggcggcg ccattcgcgt cgatggccgc gccatttttc ttcagccggg
     4261 ccatgcccag ctcaaagcct tcgaaaaagg ccgcccagtt ttgatcaact tcgcccggat
     4321 tgctttgcca gcgttcgtag ttgtcctcaa gtagcgcttc gttccaacgc gccgtgaaag
     4381 aactcttcat gtataggtgt ggagatgtga atgcgccgag cgaaaaagac ccggcgcact
     4441 cggtaaagtg agtgaaataa gcggattcta caaggatggt tttttcggcc cgctcaataa
     4501 cccaaaactt tcagtccggg aattcgttga aaatcggccg cgttccgggt caccaagggc
     4561 aggtgatgcg ccaaggctgt cgcgcagatc caaagatcat tggagccaat cagttgtccg
     4621 cgagcccgca gttcgcgaac gaggcgggag tattgccacg ccacccggcc gtccagcggc
     4681 agaatttcaa aactcgcctg ccaggccaga aaaaccgcat cggccgcggc aaaaccttcc
     4741 gaaaattctc cccaggccac cgccgaaagg taactggtct gccctgcatg cgcttcggca
     4801 aaccggcaag cggcccccgc ttccccccgg cgcctttccc gttggagatc gatcaggaaa
     4861 catgtgtcga aagtcatggc gtgggatgat tccccggatc atccggcgga gcatcctccc
     4921 gctgagccgc ctccaggcga tccagaacgg attccggcaa cggttccgtt tcgcggacca
     4981 ggttcaacaa gcgcccaagg gttcgtggcg ccgactgcca ttccgcccgt ttgatcaccc
     5041 tggaaaagga ttctcccggc gtcagccgcg ccccggccag ccggtcatac gcctccagat
     5101 cgatcgagat tgttttggtc gccatgcatg catggtacca tgcatgcata atggctgcaa
     5161 ggttgcttcc gccgtcccac aaggcgaagc ttccgtgaac tctgcttgtc acccgctgct
     5221 ccgcgggtta agggtttccg ttccaccgga atgatcgaag tcgaaaatct caccaagcgt
     5281 tattcaggcg ccgccgccct ggacggcatc tcgtttcagg tcggcaaggg cgagatcgtc
     5341 ggctttctcg gtcccaatgg cgcgggcaag agcacgacga tgcggattct ttccggattc
     5401 atgccgccca gttccggccg ggccagcgtg gcgggattcg atgtctgccg ggaatcgctc
     5461 cgggcgcgcg agcacgtggg ttacatgccg gaaaatgtgc cgctttacac cgagatgcgc
     5521 gtcggcgaat acctccgcta ccgcgccgcg ctcaagggcg tccggggccg gaaggttccc
     5581 gagcgggtca acgatgtcct cgaactctgc ggcctcgccg acgtcgcccg caaattgatc
     5641 ggcaagctct ccaagggcta ccggcagcgg gtcggcctgg cggacgccat ggtgcatgaa
     5701 ccggatttgc tcatcctgga cgagccgacc atcggcctcg atcccaacca aatccgccag
     5761 gtgcgcgatc tcatccgcaa cctcgcccgg catcacacca tcctgctcag ctcccacatc
     5821 ctttccgaag tcgagctgac ctgccatcgc gtcatcatcc tcaaccgcgg caggatcgag
     5881 gcgctcgaca cgccgcagaa tctccgccag cggctgcgtt ccggcgggga aatccggctg
     5941 gaaatccacg ccgcgaattc cggttcggcg ctgccggagc tgaaaaaaat ccccggcgtt
     6001 caatccgccc gtttcgacag cgagggcgac tggcatttgt tccacctgca aaccgaggcc
     6061 ggcgccgatc cgcgcgaggc catttttgcc cattgcgtct cgcgcggatg gaaggtccgc
     6121 gaactcacgc tcacgcgcac ctcgctcgag gatgtctttg tggaaatgac ccaaccggaa
     6181 taaccgatgc gcaaactttc catcctcttc ggccgcgagc tcgcccatta tttccgttcg
     6241 ccgctcgcct gcaccgtgct gtgtttcttc ctcgtgctga ccggatttaa tttccaatac
     6301 ggcgtctcct ccctcaacct ccagccgctg acgacgacca tcgtcgaggc ttacttcaac
     6361 acggtcttct tctggtatcc cttcgtgctc gtgtttccgc tcatcacgat gcgaactttt
     6421 tccgaggaat acaaactcgg caccatcgaa ccgctcatga ccgcgccggt gcgcgactgg
     6481 caggtggtga tggccaaata ccttggcgcg ctggtgtttt acctgattct ctggctgccg
     6541 agcctgctat attttgtgat tttccagaat acgacgggct tccgcgcggc ggaggcggtg
     6601 ggatcctact tcggcgctta ctcgatgctg ctgctcatcg gcatgtttta tatcgcgctg
     6661 ggctgcctgg cctcggcgtt gacggaaaac cagatcgtgg ccgccatcat ctccttctgc
     6721 ctgacgtgcg tgatgttttt catgtcgctg atctccttcc tgaccccgca gatcagtccg
     6781 gttttgcgcg atctgagctc gtatctttcc ccgatcgaac acatggcggg gttctcccag
     6841 ggcatctttg acacgcgccc ggtggtttat tacgtgagcc tcacggtttt caccctttac
     6901 ctgacgctgc agattttcca gtcgcgccgc tggaaaaccg cctgattctt ccatggccaa
     6961 acccgcttca acgccttccg aatcccgccg ccgcggcgtg ggccgggggg tgctcatcca
     7021 ggccgcgttt gtcgccctgc tgatccttgt cgtcaattac gcgggcttta attattattg
     7081 gcgcgccgac tg
//
LOCUS       DATDAY010000028         2944 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_2571588, whole genome shotgun sequence.
ACCESSION   DATDAY010000028 DATDAY010000000
VERSION     DATDAY010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2944)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2944)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2944
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2571588"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            144..1142
                     /locus_tag="VIS74_00615"
     CDS             144..1142
                     /locus_tag="VIS74_00615"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEY5791766.1"
                     /translation="MNPDRIQQILGDAKSKRIAVIGDLMLDRFVYGKVSRISPEAPVP
                     VVEVVREANYPGGAANVARNLAPFCAESLICGRIGCDSYGSELEQLLIEEGIATSGLI
                     RSEAVPTIVKTRIIARQQQVVRVDREIRSGAAVPVEAIVAQLAPRLEECDAVIFEDYG
                     KGFLTQTLVDALMPVLKRKGIRVTVDPNPLNPLHWPGVDIIKPNRSEAFAAAGVPLVE
                     GAAHPQSDPALAETGRRLLEKWRVPALLITLGEDGMMLFRAGREPYHTPTRAREVYDV
                     SGAGDTAIALFTAALAAGASLEEAAEISNHASGVVVAKLGTATLTPEELRASFERD"
     gene            1190..2371
                     /locus_tag="VIS74_00620"
     CDS             1190..2371
                     /locus_tag="VIS74_00620"
                     /inference="COORDINATES: protein motif:HMM:NF020084.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST N-terminal domain-containing protein"
                     /protein_id="HEY5791767.1"
                     /translation="MPPLAYSLNRQGVYDPQEVTAAAWELRQKLGRPAALAFAFVTSD
                     FLPHLEEFCELIRVDGHVTHVVGCTTSGSIQGAVEFEHQSGFSLLAVAASAGSLTALA
                     ITEEQLEAARGPADWRFRAQGLRPSGWIALLNPGEFSCDDWLKEWNAAFPQVPCAGGL
                     ASGSGDESTLAVFLDGKAIPGGVVVGIQDELRLRAVISQGCRPIGEPLTVTRAESNVI
                     YSLGSQPAYAALETAFQSLSDSEKSTARGNLFAGLASSEYLEDFKSGDFLIRNIIGAD
                     PNSGAVVIAGIPRVGQTVQYQFRDREVASQDLRDALQRPRKQGSPPVASLLFSCTGRG
                     ERLFGSRNHDAALVAEIWGAHPSAGFFCNGEIGPISGINGLHGYTASVALLEAAAPPR
                     A"
     gene            2368..>2944
                     /locus_tag="VIS74_00625"
     CDS             2368..>2944
                     /locus_tag="VIS74_00625"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEY5791768.1"
                     /translation="MKTRWFTPKRLILAAAVLAAAAGGWAWKAGDDSRRAAELPDELV
                     ETARRRDIETKLILTGEVSPAFSVDVKSEISGRVEKIDVDTGQSVHKGNSLVTIDDKD
                     LLTEKKSALTNIEGAKLDVEKKRGNYTRAKALFDEKLISKEVYENLHSDFLIAGNSLT
                     KSEASLQAVEDKLSKTRILAPADGTVLAINVS"
BASE COUNT          527 a          897 c          991 g          529 t
ORIGIN      
        1 tgccgaagga gcttcctggg caggcgcaag ggggctaatc gccaggattc ccccggcggc
       61 agcggaccac agattgattt tcatgggtga atttgcttcg catgcacggt tgggtgccgc
      121 tacaaagcgg ggcgcatcat taaatgaacc ccgatagaat acaacaaatt cttggagacg
      181 caaagtccaa gcgcatcgcc gtgatcggcg atctcatgct ggaccgcttc gtgtatggca
      241 aggtttcccg catttcgccg gaggcgcccg tgccagtggt cgaggtggtt cgcgaggcga
      301 attaccccgg cggcgccgcg aatgtggccc ggaatctggc ccccttctgc gcggaaagcc
      361 tgatttgcgg ccggattgga tgcgattcgt atggttctga attagaacag cttttgattg
      421 aagagggaat cgcgacttcc ggcctgatcc gctcggaggc ggtgcccacg atcgtcaaaa
      481 cgcggatcat cgcccgccag cagcaggtgg tccgggtgga ccgggaaatc cgttccggcg
      541 cggcggttcc ggtcgaggcg attgttgcgc aactcgctcc ccggctggag gaatgcgacg
      601 cggtaatttt tgaagactac ggcaagggat ttctgaccca gactctggtg gatgccctga
      661 tgccggtcct caagcggaag ggcatccggg tgacggtgga ccccaatccg ctgaatccgt
      721 tgcactggcc gggcgtggac atcatcaagc ccaaccgcag cgaggcattc gccgcggcgg
      781 gggtgccgct ggtcgaggga gcggcgcatc cccagtccga tccggcgctc gcggagacgg
      841 ggcgccgcct gttggaaaaa tggcgggtgc cggcgctgct gatcacgctg ggggaggatg
      901 gcatgatgct cttccgggcg ggccgggagc cgtatcacac gcccacccgg gcgcgggagg
      961 tttacgacgt gtcgggcgcc ggggataccg ccatcgccct tttcaccgcg gcgctggccg
     1021 ccggcgcctc gctggaggag gcggcggaga tttcgaacca cgccagcggg gtcgtggtgg
     1081 ccaagctcgg caccgcgacg ctgacgccgg aggaactgcg cgcgagcttt gagcgggatt
     1141 agtccattct gcgcattgac acggcgaatt ttagcaggag aattcgttaa tgccgccgct
     1201 tgcttattcc cttaatcgcc agggtgtcta tgacccgcag gaagtcactg cggcggcgtg
     1261 ggaattgcgc cagaaactcg gccgtccggc ggcgctggca tttgcctttg tcacgagcga
     1321 ttttctcccg cacctggagg agttttgcga actcattcgc gtggatggcc atgtgactca
     1381 tgtggtgggc tgcacgacct cgggcagcat ccagggcgcg gtggaatttg agcaccaaag
     1441 cggcttctcg ctgctggccg ttgcggcctc ggccggctcc ctcacggcgc tggcgatcac
     1501 cgaggagcag ctggaggcgg cccgcggccc ggcggactgg cgcttccgcg cgcagggtct
     1561 ccggcccagt ggctggatcg ccctgctgaa tccgggcgaa ttttcctgcg acgattggct
     1621 gaaggaatgg aacgccgcct ttccccaggt gccctgcgcc ggggggctcg ccagcggctc
     1681 gggcgatgaa agcacgctgg ccgtttttct cgatggcaag gccattcccg gcggcgtggt
     1741 ggtcggcatt caggacgaat tgcgcttgcg ggcggtgatc agccagggct gccgcccgat
     1801 cggggaaccg ctcacggtga cgcgcgcgga gagcaacgtg atttattccc tcggctcgca
     1861 gccggcctac gccgcgctgg agaccgcgtt ccaatccctg agcgactcgg aaaaatccac
     1921 cgcccggggc aacctctttg ccgggctcgc ctcgagtgaa tacctcgagg atttcaagtc
     1981 cggggacttc ctcattcgca acatcatcgg cgccgatccg aattcgggag cggtggtcat
     2041 cgccggcatc ccccgcgtgg gccagaccgt gcaataccag ttccgcgacc gcgaggtggc
     2101 ctcccaggat ttgcgggacg cgctccagcg cccgcggaaa caaggttccc cgccggtggc
     2161 gtccctgctt ttttcctgca ccggacgggg cgaacggctc ttcggcagcc ggaaccacga
     2221 tgcggccctc gtggccgaaa tctggggtgc ccatccctcc gccggctttt tctgcaatgg
     2281 cgaaatcggg ccgatctccg ggatcaatgg cttgcacggc tacacggcgt cggtcgcgtt
     2341 gctggaagcg gcggcacccc cccgcgcatg aagacgcgat ggttcacccc aaaacgtttg
     2401 atcctcgcgg cggccgtcct ggccgccgcc gccggcggct gggcctggaa agccggcgat
     2461 gattccaggc gcgccgccga attgccggac gaactggtgg agacggcccg gcgccgggat
     2521 atcgagacca agttgatcct taccggcgag gtgtcgccgg cattctccgt ggatgtgaaa
     2581 tccgagatca gcggccgggt ggagaagatc gacgtggaca ccggccaatc cgtccacaag
     2641 gggaattccc tggtgacgat cgatgacaag gacctgctga ccgagaaaaa aagcgcgctg
     2701 acaaacatcg agggggccaa gctcgacgtg gaaaagaagc ggggcaacta cacccgcgcc
     2761 aaggcgctgt ttgacgaaaa gctgattagt aaagaggttt atgaaaattt gcacagcgat
     2821 ttcctgatcg cgggaaattc gctgaccaaa tccgaggcca gcctgcaggc cgtggaggac
     2881 aagcttagca agacgcgcat cctcgcgccg gcggacggga cggtcctcgc catcaatgtc
     2941 agcg
//
LOCUS       DATDAY010000029         2871 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_813322, whole genome shotgun sequence.
ACCESSION   DATDAY010000029 DATDAY010000000
VERSION     DATDAY010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2871)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2871)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2871
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_813322"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..687
                     /gene="fabG"
                     /locus_tag="VIS74_00630"
     CDS             <1..687
                     /gene="fabG"
                     /locus_tag="VIS74_00630"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002442508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HEY5791769.1"
                     /translation="AIAKKFADEGAKVAIVSRTEANSQKVADGINAVHPDAAKPYAVD
                     VADFAAVQAVGEQILKDFGRADILVNNAGITRDNLAMRMSSEEWDAVLGTNLKGAFNF
                     IRAVLRPMVKQRCGRILNISSVSGLMGLAGQANYAASKAGLIGMTKSLARELASRNIT
                     VNVVAPGFIETDMTAVLNEEIRKGAQAQIPLNRFGATGDIAAAVAFFASAEAAYITGQ
                     VLAVDGGMSM"
     gene            complement(772..1374)
                     /locus_tag="VIS74_00635"
     CDS             complement(772..1374)
                     /locus_tag="VIS74_00635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5791770.1"
                     /translation="MRLSPLSRLLAIFQLLVGPGLALAQPSYSLLPEARAMRPPVMQS
                     IPGSERAVLAAVKLENGEVAYLSKEELAQLEAAPPNFETQARETAAKLLETLEPRLIR
                     SKSKVLECAVYESDSPLTAAVILAPNFLQKFENLFGPEMLVAIPSRYEVYIFPKIANR
                     IPNYTPDILSDYRAAAYPVSPEIFEIGPKGIRAVGELDDR"
     gene            1373..1978
                     /gene="ruvA"
                     /locus_tag="VIS74_00640"
     CDS             1373..1978
                     /gene="ruvA"
                     /locus_tag="VIS74_00640"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEY5791771.1"
                     /translation="MITFLEGTLVESLPTQVVVEVNGVGYQVFVPLSSYDHLPPAGSK
                     FRILTHLIVREDAHILYGFARAVERDLFRLLIQHVTGVGPKLALAVLSGMSVEIFKGC
                     VVTGDIAAISKISGVGKKTAERIVVELKDKVGVAAEWEASSQKHGPSPAEAQLHDAVL
                     ALISLGYKQVDAHKAVKAAIDRNPGSAATEALVREALRLLA"
     gene            1975..>2871
                     /locus_tag="VIS74_00645"
     CDS             1975..>2871
                     /locus_tag="VIS74_00645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5791772.1"
                     /translation="MNEELARAQKIRGLLPQGGLFHEKSWRVSPDAFELPAHLVEPLE
                     KLGYRLRKFVRACNDLYHLSVKGRQPAWIAELLDAGKPPGLVALQRERSQRNEVPRVI
                     RPDLILTDHGFIISELDNVPGGIGLTAWLNRVYSENTTADILGGANGMLEGFASIFPG
                     GDIVVSEESATYRPEMEWLARELAAFKNGGARWTVRGAGAGQAWASRVYRFFELFDLA
                     NVPAAPELQRKAAAGELSVTPPFKPALEEKLWLGLFWMKPLEDYWRRALGERTLQALR
                     RHIPYTWLLKPGELPIHAVIPRL"
BASE COUNT          543 a          903 c          906 g          519 t
ORIGIN      
        1 gccattgcca aaaaatttgc cgacgagggc gcgaaagtcg ccatcgtcag ccgcacggaa
       61 gccaactccc aaaaagtcgc ggatggcatc aacgccgttc atcccgacgc cgcgaaaccc
      121 tatgccgtgg acgttgccga ctttgccgcc gtgcaggcag tcggtgagca aattctcaag
      181 gactttggcc gggcagacat cctcgtcaac aatgccggca tcacccgcga caacctcgcc
      241 atgcgcatgt cctccgagga atgggacgcc gtgctcggca cgaatctcaa gggcgcgttt
      301 aacttcatcc gcgccgtgtt gcgcccgatg gtcaagcagc gctgcggccg catcctgaac
      361 atcagctccg tgagcggatt gatgggcctc gccggccagg cgaactacgc cgcgagcaag
      421 gccggcctga tcggcatgac caagagcctc gcccgcgagc ttgcgagccg caacatcacc
      481 gtcaacgtcg tggcgcccgg tttcatcgaa accgacatga ccgcggtgct caacgaggaa
      541 atccgcaagg gcgcgcaggc gcaaatcccg ctgaaccgct ttggcgcgac cggggacatc
      601 gccgccgccg tcgccttctt cgccagcgcc gaagccgcct acatcaccgg ccaggtgctc
      661 gccgtggacg gcggaatgag catgtaggaa ttttggattt gcgattttgg attttggatt
      721 gcggcgacat ttccccaatc gcaaatccaa aatctaaaat ccaaaatgga ttcatcggtc
      781 gtccagttcg ccgaccgcgc gaatcccctt ggggccgatt tcgaaaatct cggggctgac
      841 cggatacgcc gccgcgcgat agtcggacag gatatccggc gtgtaattgg gaatgcggtt
      901 ggcgattttc ggaaagatgt aaacctcgta acgggaggga atggccacca gcatttccgg
      961 tccgaagagg ttttcgaatt tctgaagaaa attcggcgcg aggatcacgg cggcggtgag
     1021 gggggaatcc gactcataca cggcgcattc gagcaccttg gatttgctgc ggatcaagcg
     1081 cggttccagg gtctccagca gcttggcggc ggtctcccgc gcctgggttt caaaattggg
     1141 cggagccgct tcgagctggg cgagttcctc tttggataaa taagcgactt cgccgttttc
     1201 cagcttcacc gccgccagga cggcccgttc cgagcccgga atggattgca tgaccggcgg
     1261 gcgcatggcg cgcgcctccg gcagcaggct gtaggacggc tgggccagcg ccaagcccgg
     1321 cccgaccaat aactggaata ttgccaaaag cctgctaaga ggactgagtc gcatgattac
     1381 gtttttggaa ggcacgttgg tggaatcgct gcccacgcag gtggtcgttg aagtgaatgg
     1441 cgtcggctat caagtgtttg ttccgctttc cagctacgat cacctgcctc cggcgggaag
     1501 taaattccgg attctcacgc atctgatcgt tcgggaggat gcccacattc tctacggctt
     1561 cgcgcgggcg gtggagcggg acttattccg cctgctcatc cagcacgtga cgggcgtggg
     1621 gccgaagctc gcgctggccg tgctcagcgg catgtccgtc gagattttca agggctgcgt
     1681 agtgaccgga gacatcgcgg ccatttccaa aatcagcggc gtgggcaaaa aaaccgccga
     1741 gcggatcgtg gtcgagctca aggacaaggt gggcgtcgcc gcggaatggg aggcttccag
     1801 ccagaaacac ggcccgtcgc ccgccgaggc gcagctgcac gatgcggtgc tcgcgctcat
     1861 ttcgctgggc tacaagcagg tggacgcgca caaggcggtg aaggccgcga tcgaccgcaa
     1921 tcccggctcg gccgccaccg aggcgctggt gcgcgaagcc ctgaggttgc tggcatgaac
     1981 gaggaactgg cccgggccca aaaaatccgc ggcctgcttc cgcagggcgg gctgtttcac
     2041 gaaaagagct ggcgcgtctc gccggacgcc ttcgagctgc cggctcatct cgtcgagccg
     2101 ctggagaaat tgggctatcg cctgcgcaaa ttcgtccggg cctgcaacga cctgtatcac
     2161 ctcagcgtca agggccggca gcccgcgtgg atcgcggagc tcctcgatgc gggcaagccg
     2221 cccgggctgg tcgcgctgca acgcgagcgc tcgcagcgga acgaggttcc ccgcgtcatc
     2281 cgcccggatt tgatcctcac cgatcacggc ttcatcattt ccgaactcga caatgtcccc
     2341 ggcggcatcg gcctcaccgc ctggctgaat cgcgtctatt ccgaaaacac gaccgcggac
     2401 atcctcggcg gagcgaatgg catgctcgaa gggttcgcgt ccattttccc gggcggcgac
     2461 atcgtggtct ccgaggaatc cgcgacctat cgccccgaaa tggaatggct cgcgcgcgag
     2521 ctggccgcgt tcaagaacgg cggcgccagg tggaccgtgc gtggcgcggg cgccggccag
     2581 gcctgggctt cgcgggttta ccgcttcttt gaattgttcg atctggcgaa cgttcccgcc
     2641 gcgccggaac tccagcggaa agccgcggcg ggcgagcttt ccgtgacgcc gcccttcaaa
     2701 cccgcgctgg aggaaaagct ctggctcggg cttttctgga tgaagccgct ggaggattat
     2761 tggcgccgcg cgctcgggga acgaaccctc caggcgctgc gccgccacat tccctacacc
     2821 tggctcctca agccgggcga gctgccgatc cacgccgtga tcccgcgcct g
//
LOCUS       DATDAY010000030         2024 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_967231, whole genome shotgun sequence.
ACCESSION   DATDAY010000030 DATDAY010000000
VERSION     DATDAY010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2024)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2024)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2024
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_967231"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(49..837)
                     /gene="folE2"
                     /locus_tag="VIS74_00650"
     CDS             complement(49..837)
                     /gene="folE2"
                     /locus_tag="VIS74_00650"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012536877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0035998 - 7,8-dihydroneopterin
                     3'-triphosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase FolE2"
                     /protein_id="HEY5791773.1"
                     /translation="MAKHVKLKDTQSEPDFRQLAIDRVGVKGLRYPIQIRDRKKGSEQ
                     STVATVQLTVDLPHHFKGTHMSRFVEVLNSHGSVLHVSNIRDILAQLRKRLDSRHAHV
                     SFEFPFFLEKAAPVTGAAGLIDYTVRFDASLEGRKLDFVVTVVVPVTTLCPCSKAISA
                     RGAHNQRGHVTFALRSTEPIWIEDMIRMVEESASSELYSLLKRPDEKAVTERAYDNPV
                     FVEDLVRNVALRADGDKRIEWYRVEAENFESIHNHSAYALIEKK"
     gene            903..>2024
                     /locus_tag="VIS74_00655"
     CDS             903..>2024
                     /locus_tag="VIS74_00655"
                     /inference="COORDINATES: protein motif:HMM:NF012589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HEY5791774.1"
                     /translation="MARALILAFDIGTSSFRTALFDPHGRRVPETLAQQTYPLLTNPE
                     GQAELDPAALEKAARACLARTLAAFRQRNKKERITATGASCFWHSLLAADSAGVPLSP
                     IYTWAESRCKTEAEKLRQKIDERAYHQSSGAMLRASFWPAKLAWLKKTRPALFSQAAA
                     WLSPADWLFSRFCTGWHPSYSMASGTGLFNRKSNDWDDSLLRRFGISRSQLGVISDAP
                     LATAPEFSQTFPELGETQWLPPIGDGAASNLGCGATAPGQAAINFGTSAAIRVISTDE
                     RPKVPFGLFAYRVDARRWLIGGAISNAGNLRAWALQNLQLPDDEALEKAFQNHVPASL
                     TALPFLAAERAPSWPEEVPAALLGFNLSTTAVDIFAALTE"
BASE COUNT          371 a          749 c          528 g          376 t
ORIGIN      
        1 ttttataggt tccatgggac ctataagacc cataggactt ataaggttct atttcttctc
       61 aatcagcgca taagcgctgt ggttatgaat tgactcgaaa ttctccgcct cgacccggta
      121 ccactcgatc cgcttgtccc catcggcgcg cagcgccacg ttcctcacca gatcctccac
      181 gaataccggg ttgtcatacg cccgctcggt cacggctttt tcgtcgggcc gcttgagcag
      241 gctgtaaagc tcgctgctcg cgctctcctc caccatgcgg atcatgtcct cgatccagat
      301 cggctcggtg gaacgcagcg cgaacgtcac gtgcccgcgc tgattatgcg cgccccgggc
      361 gctgatcgcc ttggaacagg ggcacagcgt cgtcaccggc accaccacgg tgaccacaaa
      421 gtccagcttc cggccttcca gcgaggcatc aaaccgcacc gtgtaatcaa tgagcccggc
      481 cgcgccggtc accggagccg ctttttccag aaagaacgga aactcaaaac tcacgtgcgc
      541 atgccgggaa tccagccgtt tccgcagctg cgccaggatg tcgcggatat tgctcacgtg
      601 caaaaccgag ccatgggaat tgagcacctc cacgaaccgg ctcatgtgcg tgcccttgaa
      661 atgatgcggc aaatccaccg tcagctgcac cgtcgccacc gtgctctgct ccgagccctt
      721 tttccggtcc cggatctgaa tcgggtaacg caaccccttc acccccaccc ggtcaatcgc
      781 gagctgccgg aaatccggct cgctctgggt gtccttcaac ttcacatgct tggccatcgc
      841 caaaggctac cgaatttctt tgtcagccgg atataagaaa atagcatgac tccccacaca
      901 ccatggcgcg cgctctgatt ctggcttttg acatcggcac ctcctccttc cgcaccgctc
      961 tcttcgatcc ccacggccgg cgcgtcccgg aaaccctcgc ccagcaaacc tacccgctcc
     1021 tcaccaatcc ggaaggccag gcggaactcg atcccgccgc gctggaaaaa gccgcccgcg
     1081 cctgcctggc ccgcaccctg gccgcctttc ggcaacgaaa caaaaaggaa cgcatcaccg
     1141 ccaccggcgc ctcctgtttc tggcacagcc tgctggcggc ggactccgcc ggcgtccccc
     1201 tcagccccat ctacacctgg gcggaatccc gctgcaaaac cgaggcggaa aaactccgcc
     1261 aaaaaatcga cgaacgcgcc taccatcaat cctccggcgc catgctgcgc gcttccttct
     1321 ggccggccaa gctggcctgg ttgaaaaaga cccgcccggc cctgttttcc caggccgccg
     1381 cgtggctttc gccggccgac tggctcttca gccggttttg caccggctgg catcccagct
     1441 actccatggc cagcggcacc ggcctgttta atcgaaaatc caacgattgg gacgactccc
     1501 tgctccggcg cttcggcatt tcccggagcc agctcggtgt catctccgac gcgcccctcg
     1561 ccacggcgcc ggaattttcg cagacctttc ccgagctcgg ggaaacccaa tggctgccgc
     1621 ccatcggcga cggcgcggcc agcaatctcg gctgcggcgc gaccgccccc ggccaggccg
     1681 ccatcaattt cggcaccagc gccgccattc gcgtcatctc cacggacgag cgcccgaagg
     1741 tgcctttcgg gcttttcgcc taccgcgtgg acgcccgccg ctggctcatc ggcggcgcga
     1801 tcagcaatgc gggcaatctc cgcgcctggg cgctgcaaaa tctgcaattg cccgacgacg
     1861 aagccctcga gaaagccttc cagaaccacg ttccggcgtc cctgaccgcg cttcccttcc
     1921 tcgccgccga gcgcgccccc agctggccgg aggaggtgcc ggcggcattg ctgggattta
     1981 atctctccac gaccgcggtg gacattttcg ccgcgctcac cgag
//
LOCUS       DATDAY010000031         3129 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_967030, whole genome shotgun sequence.
ACCESSION   DATDAY010000031 DATDAY010000000
VERSION     DATDAY010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 3129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3129
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_967030"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            83..2023
                     /gene="shc"
                     /locus_tag="VIS74_00660"
     CDS             83..2023
                     /gene="shc"
                     /locus_tag="VIS74_00660"
                     /EC_number="5.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006983961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051007 - squalene-hopene cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0019746 - hopanoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene--hopene cyclase"
                     /protein_id="HEY5791775.1"
                     /translation="MNPARNIVALDPAGECLPPDSLLHAIGSAQEYLLSIQKPEGYWI
                     GELEVDCTLCADYIAYMHWRGQVDASLQEKCATHVRRRQLADGGWNIYVDGPSELTAS
                     IKAYFALKLAGDSPDAPWMREARANVLRLGGIPQLNTYGKLYLALLGQFPWEHLPSIP
                     SEFFLLPKWFFFNLYEMSSWTRTIIAPLAILNHYKPTRRLPAHLQIHELYPAGVEGSD
                     LSLKRDRRFWAWRNFFLRVDRAIKFYEKRPIAALRRYALKAVEKWMTDRMGEGSNGLS
                     AIYPAMLNSMMALRCLGYPADHPLVKKADADFAGLFVHDPKDFRIQPCLSPVWDTAIT
                     TIALGESGLEPTHPALQRAATWLEDKEVRHRRGDWAQSKNPHPEASGWAFEFNNDFYP
                     DTDDTMMVLMALRLTKPRDAARHAATQERALKWLLSFQCDDGGWAAFDKNVTQRWLED
                     VPFADHNAILDPTCSDLTARVLELLGKAGFSRDHWQVRRALAQIKRTQEPDGSWYGRW
                     GVNYIYGTWQILRGLASIGENMTQPWIQRGRDWLESCQNEDGGWGETCASYDDPALKG
                     RGPSTASQTAWAVMGLCACGVRRPSIESGIRWLLRQQNEDGSWTETATTGTGFPRVFY
                     LKYDMYRLHFPLLALAACQKLR"
     gene            2020..2403
                     /locus_tag="VIS74_00665"
     CDS             2020..2403
                     /locus_tag="VIS74_00665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5791776.1"
                     /translation="MKLFPPALLLVLLMSGCSALNEMQAAQTGRTLSASGFRALPANT
                     PERAASLARLKPYQINRKTRSGAVYYLYPDPQQGILFVGDQSQYTAYQGYATQAAGDE
                     NRRIDELYAADTLGWADWKIWGEAW"
     gene            2397..3125
                     /locus_tag="VIS74_00670"
     CDS             2397..3125
                     /locus_tag="VIS74_00670"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEY5791777.1"
                     /translation="MVGESSIPGSPVIRPLVIGHRGAAGHEVENTRVAFLKGLALGAD
                     GVECDVRATSDGKLVIFHDESLLRLAGLPERLNRLTLADLQKRVRLGESGEPILTLEE
                     LCSMPEVQNAWLFIELKEPRLEAEAIRIVQKTRDAQNTVLGSFRPETVRALTAVNFPL
                     PLLLSETFAMPPAGVQIAGLRHEALDPAKVTGLHRQQLQVWSWTVNETDDIQRMISCR
                     VDGLITDYPDRAIRELNRLLPLSD"
BASE COUNT          568 a         1041 c          972 g          548 t
ORIGIN      
        1 ccaacgtcaa cgccttcaac ggagtcaccg ccggcaacgg ccataaaacc ggccgcccgg
       61 cccgaacgac cccggcggcg gcgtgaaccc cgcccgcaac attgtcgcgc tggatccggc
      121 gggcgaatgc ctcccgcccg actcgctgct tcatgcaatc ggaagcgcgc aggaatacct
      181 cctgtccatt caaaagccgg agggttattg gattggcgaa ctggaggtgg attgcacgct
      241 ctgcgcggat tacatcgcct acatgcactg gcgcggccag gtggatgcca gccttcagga
      301 aaaatgcgcc acgcatgtcc gccggcggca gctggcggac ggcggctgga acatctacgt
      361 ggatggcccg agcgagctga ccgcctccat caaggcctat ttcgcgctca agctggccgg
      421 ggattcgccc gacgcgccgt ggatgcgcga ggcgcgggcc aacgtcctcc ggctcggcgg
      481 cattccgcag ctgaacacct acggcaagct gtatctcgcg ctgctcggcc agttcccgtg
      541 ggaacacctg ccctcgatcc cctccgagtt tttcctgctg ccgaagtggt tcttttttaa
      601 cctctacgaa atgtcctcgt ggacgcgcac gatcatcgcg ccgctggcca ttctcaatca
      661 ctacaaaccc acgcgccggc tgcccgcgca tttgcagatc cacgagctgt atcccgccgg
      721 cgtcgagggt tccgatctct cgctcaagcg cgaccgccgc ttctgggcgt ggcggaattt
      781 tttcctccgc gtggatcgcg ccatcaagtt ctacgaaaaa cggccgatcg ccgcattgcg
      841 gcggtacgcg ctgaaggcgg tggaaaaatg gatgacggat cgaatgggcg aaggcagcaa
      901 cgggctttcc gccatttatc cggccatgct caactccatg atggcgctgc ggtgcctggg
      961 ttaccccgcc gatcatccgc tggtgaaaaa agccgacgcg gacttcgccg ggctgttcgt
     1021 gcacgatccg aaggatttcc gcattcagcc ctgcctgtct cccgtgtggg acacggcgat
     1081 caccaccatc gcgctcggcg aatcgggcct tgaaccgacc catcccgcct tgcagcgggc
     1141 ggccacgtgg ctggaggaca aggaagtgcg gcatcgccgc ggggattggg cgcagagcaa
     1201 gaacccccac cccgaagcca gcggctgggc gtttgagttc aacaatgatt tttaccccga
     1261 caccgatgat accatgatgg tgctcatggc gctgcggctg accaagccgc gcgacgccgc
     1321 gcgccacgcc gccacgcagg agcgggcgct gaagtggctg ctgagcttcc agtgcgacga
     1381 cggcggctgg gccgcgttcg acaagaatgt cacccagcgc tggctggagg acgtgccctt
     1441 cgcggatcac aacgccatcc tcgatcccac ctgcagcgac ctgaccgcgc gcgtgctgga
     1501 gctgctgggc aaggccggat tttcgcgcga ccattggcag gtgcggcgcg cgctggccca
     1561 gatcaaacgc acgcaggagc ccgacggctc ctggtatggc cgctgggggg tcaattacat
     1621 ttatggcacc tggcagattc tccgcgggct ggcgtccatc ggcgagaaca tgacccagcc
     1681 atggattcag cgcggccggg actggctgga gagctgccag aacgaggacg gcggctgggg
     1741 cgaaacctgc gcctcgtatg acgatccggc cctgaagggc cgcggcccga gcacggccag
     1801 ccagacggct tgggcggtga tggggctgtg cgcctgcggc gtccggcgtc cgtccatcga
     1861 atccggcatc cgctggctgc tccgccagca gaacgaggac ggctcgtgga cggaaaccgc
     1921 gacgaccggc acgggatttc cccgcgtctt ttatttgaaa tacgacatgt atcgcctgca
     1981 cttcccgcta ctggcgctcg ccgcctgcca aaaacttcga tgaagttatt ccccccggca
     2041 cttctcctcg tccttttaat gtccggttgc tcggcattga acgaaatgca ggccgcgcag
     2101 accgggcgga cgctcagcgc ctccggcttc cgcgcgcttc ccgccaatac cccggaacgc
     2161 gccgcttcgc tcgcccggct caaaccctat caaatcaatc gcaaaacccg gagcggcgcc
     2221 gtttattacc tctatcccga tccgcaacag ggaattctct tcgtcggaga tcaatcccaa
     2281 tacaccgcct atcagggtta cgccacgcag gcggccgggg acgaaaaccg gaggattgac
     2341 gagctatacg cggcggacac gctgggctgg gcggattgga aaatctgggg ggaagcgtgg
     2401 taggcgaatc ctccatcccg ggctcccccg tcatccgccc gctggtcatc ggccatcgcg
     2461 gcgcggccgg gcatgaggtt gaaaataccc gcgtcgcctt cctgaaaggg ttggcgctgg
     2521 gggccgatgg cgtggagtgc gacgtccgcg ccacgagcga cggaaagctg gtgatctttc
     2581 acgacgaatc cctgcttcgc ctggcgggcc tgcccgagcg gctgaaccgg ctcacgctgg
     2641 ccgatctgca aaagcgcgtg cggctgggcg aatccgggga gcccattctg acgctggagg
     2701 aattgtgctc catgccggaa gttcagaacg cctggctgtt catcgagctc aaggagccgc
     2761 gcctggaggc ggaagccatc cgcatcgtgc aaaaaacgcg cgacgcccaa aacaccgtgc
     2821 tcggctcctt ccggccggaa accgtccgcg cgctgaccgc ggtaaatttt cccctgccgc
     2881 tgctgctttc cgaaaccttt gccatgcccc ccgccggcgt gcagatcgcc ggcttgcggc
     2941 atgaggcgct ggatcccgcg aaagtcaccg gactgcatcg ccagcagctc caggtctgga
     3001 gctggaccgt gaacgaaacc gatgatattc agcggatgat ttcctgccgg gtggacgggt
     3061 tgattaccga ttatcccgat cgcgccatcc gcgagctgaa ccggctcctt ccgttgtccg
     3121 actgaagcg
//
LOCUS       DATDAY010000032         4244 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_2024988, whole genome shotgun sequence.
ACCESSION   DATDAY010000032 DATDAY010000000
VERSION     DATDAY010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 4244)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4244)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4244
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2024988"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..328
                     /locus_tag="VIS74_00675"
     CDS             <1..328
                     /locus_tag="VIS74_00675"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEY5791778.1"
                     /translation="DLPTRKAIDRHLREARVRVRHAMEFDSVETVKRTVEIENAISIV
                     PETAVRDEVAAGTLVALPVEHTPALARPLGVIVKKSALGLPVIREFLAMLEKFDLGGE
                     RSRSLG"
     gene            366..1232
                     /gene="mtnP"
                     /locus_tag="VIS74_00680"
     CDS             366..1232
                     /gene="mtnP"
                     /locus_tag="VIS74_00680"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017061 - S-methyl-5-thioadenosine
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HEY5791779.1"
                     /translation="MKTENAIAIIGGSGLDQLPGFHQEEELFLETPFGAPSDVIRGGE
                     FQDRSLYFLPRHGRGHRIAPHEINHRANLYALRSLGVRWILSVTAVGSLQERHRPREV
                     VLIDQFFDRMSGRADHTFFGNGIVAHVSLADPICPELRACLAEACRKTGAAFHDGGTY
                     VNMDGPAFSTRAESETYRQLGFDVIGMTNFPEAKLAREAEMSLATLAMVTDYDCWKTG
                     EAPVTAGAIYAHLQANAETARAVIAEALPLIPRTPDWPAHRSLENALATPRNYWPEKT
                     VQNLSVILSRFV"
     gene            1271..2194
                     /locus_tag="VIS74_00685"
     CDS             1271..2194
                     /locus_tag="VIS74_00685"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEY5791780.1"
                     /translation="MKHTGGPIFLATTVFVLTACQSVHTQTNPSLTREAPPVMSIHAQ
                     PPAAAPTPSVETPTRLTLTPPPPESAPVASTDKPHVPAGPRFSYSSVVVSEPYVAITF
                     DDGPSAGLTPKLLDMLKERGIPATFYVVGTNAAAHPEILQRMIAEGHEIGNHSWSHPA
                     LTRVGGDGVKNQMDKTSAAIHAATGNYPATMRPPYGATSAYLTRRLNEEFGLKVIMWS
                     VDPLDWKYRNADRVANAIITSTKPGDIILAHDIHASTVAAMPRTLDALQAKGFKFVTV
                     STLLKMDEGSQLTQHPSPVQMPTATVLPANP"
     gene            2294..3034
                     /locus_tag="VIS74_00690"
     CDS             2294..3034
                     /locus_tag="VIS74_00690"
                     /inference="COORDINATES: protein motif:HMM:NF019225.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HEY5791781.1"
                     /translation="MTRAIFSGFLAVAAMGLAHATPSGLYDPFGRADAGGVNYAVWDT
                     FNEHYSSGSGAGTLYTYSGNATPSSNLTGLSLTQSSAHINAATGGGAAQGAGLLSGGD
                     IYYSSTRAQSWTLNATTSIDLTTMAFQIKTANVGGVVDSLFVPTLAGVGAAAYFRSLN
                     TGEQINGFDAYVIEYRWTDLEIAAGSNLSIGFSLAGGSSGNFTRKPVDFVALDVATVP
                     EPASSVLWLTGLGLIAWLRRRPSRTARA"
     gene            3061..3819
                     /locus_tag="VIS74_00695"
     CDS             3061..3819
                     /locus_tag="VIS74_00695"
                     /inference="COORDINATES: protein motif:HMM:NF019225.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HEY5791782.1"
                     /translation="MKKTFLSILTGLALGTGVSHAVTTGLQSDLVSGGSTSYALWDVF
                     PGPYRVGPGAPAGGTAISAWPYTFTYSSSAAPATLTATATASAPFANYITGPTDADGG
                     TIPGNRDLFYTFFAAVDWKIQSISTTDVTEMALQISYDTASTLDFSSITLTGNSGGVP
                     APVFTRGDEFTTAGGYNRAVAAWVWTGLDIHAGENFSLAFSNPTSSHFGLDAIRLDTN
                     AEMVPEPSSWLLLGLGAGFILWQRRKRHPSTQLR"
     gene            3825..>4244
                     /locus_tag="VIS74_00700"
     CDS             3825..>4244
                     /locus_tag="VIS74_00700"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEY5791783.1"
                     /translation="MTRNSPAPSRRKPRGFTLVELLAAVGILAVLGTLVFTASRNASD
                     KTKLTREIAAAKTLISAYQLAAADNNGKLLPGMDFTLNKVWFEAGNKNVTGHAAQRYP
                     FRLSPYFNDRLKGTLLIRQKSSQVNGDYAISVYPAFGI"
BASE COUNT          785 a         1480 c         1209 g          770 t
ORIGIN      
        1 cgatctgccg acgcgcaagg ccatcgaccg gcatctgcgc gaggcccggg tccgggttcg
       61 ccatgccatg gaatttgaca gcgtggaaac ggtcaaacgc acggtggaaa tcgaaaacgc
      121 catctccatc gttcccgaaa ccgcagtccg ggacgaggtg gcggcgggaa cgctggtggc
      181 gctgccggtc gaacacaccc cggcgctcgc ccgcccgctc ggcgtcatcg tcaaaaaaag
      241 cgccctcggc cttccggtca tccgcgaatt cctggccatg ctggaaaaat tcgatctggg
      301 aggcgaacgg tcgcggtcgc tgggatgacc tcccggattt tcattcaccg agttgcaccg
      361 attttatgaa aacagaaaac gccattgcga ttatcggagg gagcgggctg gatcagcttc
      421 cggggtttca ccaggaggag gagctgtttc tcgaaacgcc gtttggcgcg ccttcggacg
      481 tgatccgggg cggtgagttt caggatcgtt cgctctattt tcttccccgc cacgggcgcg
      541 gccaccggat cgcgccgcac gagatcaacc accgggccaa tctttacgcc ctgcgctcgc
      601 tgggcgtccg gtggatcctc agcgtgaccg ccgtcggcag cctgcaggaa cgccaccgcc
      661 cgcgcgaggt ggtgctgatc gatcaatttt tcgaccggat gagcgggcgc gcggatcaca
      721 ccttctttgg caacggcatt gtcgcccacg tcagcctggc cgatccgatc tgcccggagc
      781 tgcgcgcgtg cctcgccgaa gcctgccgca agaccggcgc ggcttttcac gatggcggaa
      841 cctacgtgaa catggacggc ccggccttct ccacccgggc ggaatccgag acctaccgcc
      901 agctcggatt cgacgtgatc ggcatgacca atttccccga agccaagctg gcccgggagg
      961 cggaaatgtc cctggccacg ctcgccatgg tcacggacta cgattgctgg aagaccggcg
     1021 aggcgcccgt caccgccggc gccatttacg cccatcttca ggccaatgcg gaaaccgccc
     1081 gcgcggtgat cgccgaggcg cttccgctca ttccccggac cccggactgg ccggcccacc
     1141 gctcgctgga gaacgccctg gccactccgc gcaactattg gccggaaaag acggtgcaaa
     1201 atctcagtgt gattttgagc cgctttgtgt aggttggaaa atgaccggca aaaagggcat
     1261 ttctcatttt atgaaacaca ctggcggacc gattttcctc gcaaccaccg tcttcgttct
     1321 gacggcgtgc cagtccgttc atacccaaac caacccctcc ctcacccgcg aggcgccgcc
     1381 ggtcatgagc attcacgccc agccgccggc cgccgcgccc acgccctcgg tggaaacccc
     1441 cacccgcctg acgctcaccc cgccgcctcc ggaatccgcg ccggtcgcct ccaccgacaa
     1501 gccgcacgtg ccggccggcc cgagattcag ctattcctcc gtggtggtga gcgagcccta
     1561 cgtcgccatc actttcgacg acggcccgtc cgccgggttg accccgaagc tgctcgacat
     1621 gctgaaggag cgcggcattc ccgcgacgtt ctacgtggtc ggcaccaatg ccgccgccca
     1681 tccggaaatc ctgcaacgca tgatcgccga aggccatgag atcggcaatc actcgtggag
     1741 ccatcccgcc ctgacccggg tcggcgggga cggcgtgaaa aaccagatgg ataaaaccag
     1801 cgccgccatt catgccgcca ccggaaatta tcccgccacc atgcggccgc cttacggcgc
     1861 gaccagcgct tacctcaccc gccgcctcaa tgaggaattc ggcctgaagg tcatcatgtg
     1921 gtcggtggat ccgctggact ggaaataccg caacgccgac cgggtcgcga atgccatcat
     1981 caccagcacc aagcccgggg atatcatcct ggcgcacgac atccacgcca gcacggtggc
     2041 cgcgatgccc cgaacgctgg atgcgctcca ggccaagggc tttaagtttg tgaccgtatc
     2101 aacgttgtta aaaatggacg agggctccca attgacccag cacccctcgc cggtgcagat
     2161 gcccacggcc accgtcctgc cggcgaatcc ctgagccacc gcggcctcgc gccgtgcggt
     2221 gattacaaat tcgcgccaat ctttcgcaag gcgccgttag cgggaatttc aaaaatcggg
     2281 aaaatgccgc tccatgaccc gagcgatatt ttccggattc ctggccgtgg ccgccatggg
     2341 gctggcgcat gccaccccga gcggccttta cgatcccttt ggccgcgcgg atgccggcgg
     2401 cgtgaattac gccgtctggg acacgttcaa tgaacactac agctccggca gcggcgccgg
     2461 caccctctac acgtattcgg gaaacgccac cccgtcgtcc aatctcaccg ggctctcgct
     2521 cacccaaagc agcgcgcaca tcaatgccgc caccggcggc ggcgcggccc agggcgccgg
     2581 gctgctgagc ggcggagaca tttattattc gtccacccga gcgcaaagct ggacgctgaa
     2641 cgcgaccact tcgattgacc tcacgacgat ggcgttccag atcaagaccg ccaacgtggg
     2701 cggcgtggtg gattcgcttt tcgtcccgac gctcgccggc gtgggagcgg cggcctactt
     2761 tcgcagcctc aacaccgggg agcagatcaa cggttttgat gcctacgtca tcgaataccg
     2821 ctggacggat ctcgaaatcg ccgcgggttc caacctcagc atcggcttct ccctggcggg
     2881 cggatcgagc ggcaatttca cccggaagcc ggtggacttc gtggcgctgg atgtcgcgac
     2941 ggtcccggaa cccgcttcct cggttttgtg gttaaccggg ctcggtttga tcgcctggct
     3001 cagacgccgg ccttcccgga ccgcccgcgc ctgatcggtt tgatcctcca tttatcccct
     3061 atgaaaaaaa cgtttctttc catcctgacc ggcctggccc tgggaaccgg ggtttcccac
     3121 gccgtcacca ccggcctgca atcggatctc gtttccggcg gttcgaccag ctacgccctt
     3181 tgggatgttt tccccgggcc ttaccgggta ggcccagggg ctccggcggg gggaacggcc
     3241 atttccgcct ggccttacac gtttacctac agctccagcg cggctcccgc caccttgacg
     3301 gcaacggcca cggcgtccgc tccctttgcg aactacatca ccgggcccac cgatgcggat
     3361 ggcggaacga ttcccggcaa ccgggactta ttctacacct tcttcgcggc ggtggattgg
     3421 aaaatccagt cgatctccac gacggacgtc accgagatgg cgctgcaaat cagctacgac
     3481 accgcttcga cgcttgattt cagctcgatc accctgacgg gaaattccgg aggcgttccc
     3541 gccccggtgt tcacccgcgg ggacgagttt accacggccg gcggctacaa ccgcgccgtg
     3601 gccgcctggg tatggaccgg gctcgacatc cacgccggcg aaaatttttc cctggctttt
     3661 tccaatccca cctcctcgca cttcgggctc gatgccattc gcctggacac caacgccgag
     3721 atggttccgg aaccttcctc ctggctgctg ctggggctgg gcgccgggtt catcctctgg
     3781 cagcggcgga agcgccaccc aagcacccaa ctccgctgac gcgcatgacc cgaaattctc
     3841 cggccccttc ccgccgaaaa ccgcgcggat tcaccttggt cgaacttctg gccgcggtcg
     3901 gcatcctcgc ggtcctggga acccttgtgt tcaccgcatc ccggaatgcc agcgacaaaa
     3961 ccaagctcac gcgggagatt gccgccgcca aaaccctgat ttccgcctac caactcgcgg
     4021 ccgccgacaa caatgggaaa ctccttcccg gcatggactt cacccttaac aaagtgtggt
     4081 ttgaagccgg caacaaaaac gtcaccgggc acgcggcgca acgttatccc ttccggcttt
     4141 ccccctactt caatgaccgg ttgaagggaa ccctcctcat ccgccaaaaa agctcgcagg
     4201 tgaatgggga ttacgccatc agcgtttacc cggctttcgg catc
//
LOCUS       DATDAY010000033         3956 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_83260, whole genome shotgun sequence.
ACCESSION   DATDAY010000033 DATDAY010000000
VERSION     DATDAY010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 3956)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3956)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3956
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_83260"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..486)
                     /gene="rpsD"
                     /locus_tag="VIS74_00705"
     CDS             complement(<1..486)
                     /gene="rpsD"
                     /locus_tag="VIS74_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEY5791784.1"
                     /translation="MARYTGPKTKISRRYGVFISGSPKAFERKSYPPGMHGPKGSRRK
                     LSEYATALAEKQKLRHQYGVLERQFRRYFAIASNKRGVTGEILLQLLETRLDNVVYRM
                     GFAKTLRGARQIVAHGHVAVNGRKSDVPSMNLKPGDKVAIRTKDGSRRLAQRGLEATQ
                     IV"
     gene            complement(528..899)
                     /gene="rpsK"
                     /locus_tag="VIS74_00710"
     CDS             complement(528..899)
                     /gene="rpsK"
                     /locus_tag="VIS74_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004074888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEY5791785.1"
                     /translation="MVRAKGAKNILLGIAHVSATFNNTIVSITDQRGAVIGWSSAGKC
                     GFKGSRKSTAYAAQMVAQDACRQAMGHGLKEVEVRVKGPGSGRESAVRAIQAIGLEIS
                     VIRDVTPVPHNGCRPPKQRRV"
     gene            complement(1165..1560)
                     /gene="rpsM"
                     /locus_tag="VIS74_00715"
     CDS             complement(1165..1560)
                     /gene="rpsM"
                     /locus_tag="VIS74_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEY5791786.1"
                     /translation="MPRLLGVDIPGDKRIEASLPYIYGIGPSNTKKVLELANIDPNIR
                     AKDLSPEQLNAIIQAINQSKILIEGDLRRDLQQNLKRLQAINCYRGIRHRRGLPVRGQ
                     RTGTNARTRKGPRKTVGVARNPNAKAGKV"
     gene            complement(1680..1793)
                     /gene="rpmJ"
                     /locus_tag="VIS74_00720"
     CDS             complement(1680..1793)
                     /gene="rpmJ"
                     /locus_tag="VIS74_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HEY5791787.1"
                     /translation="MKVRASVKRLCESCKIVRRKNVVRVVCKDPRHKQRQG"
     gene            complement(1824..2042)
                     /gene="infA"
                     /locus_tag="VIS74_00725"
     CDS             complement(1824..2042)
                     /gene="infA"
                     /locus_tag="VIS74_00725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012039277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEY5791788.1"
                     /translation="MAEKDAIEVSGKVVELLPNTMFRVELANGRRVLAHISGKMRMHF
                     IRLLPGDKVMVEMSPYDLTKGRITYRQK"
     gene            complement(2015..2779)
                     /gene="map"
                     /locus_tag="VIS74_00730"
     CDS             complement(2015..2779)
                     /gene="map"
                     /locus_tag="VIS74_00730"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HEY5791789.1"
                     /translation="MIPIKTPAEIEKMRKAGEAAAHVLNALADRIAPGVTTGEIDRLA
                     GKLMAEAGVRSAFLGYHKFPGNICISLNEEVVHGIGGPRKINYGDIVKLDVGIVRDGW
                     IGDTAMSLPVGVIDPATERLLTLTEQILVDAIPLARAGRRLGDMCAFIEEKAVAAGYG
                     VVREFVGHGVGRRLHEEPQVPNFGKRGTGPKLKAGMTLAVEPMINLGKAAVRMLSDQW
                     TVVSADGQPSAHFEHTILITNDAPEVLTWPRKTLLK"
     gene            complement(2776..3435)
                     /locus_tag="VIS74_00735"
     CDS             complement(2776..3435)
                     /locus_tag="VIS74_00735"
                     /inference="COORDINATES: protein motif:HMM:TIGR01351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside monophosphate kinase"
                     /protein_id="HEY5791790.1"
                     /translation="MNTRIVLLGPPASGKGTLAALIAEKYGIPSASTGALLREEEHQN
                     TALGQAARAFTREGKYFPDEMALAVVERWLDAHASARGFVLDGFPRTLAQAGKFDEDL
                     AERHGELDIAINLELGEAEIRRRIESRLTCPRCAATYRNGGDGAREGGECPNCGAVLQ
                     RRRDDTLETLSERLRQHNQLTEPVVEYYRQTGRLRTVDAAPGSAAVFAVISQILEGVP
                     A"
     gene            complement(3452..>3956)
                     /locus_tag="VIS74_00740"
     CDS             complement(3452..>3956)
                     /locus_tag="VIS74_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SecY family transport protein"
                     /protein_id="HEY5791791.1"
                     /translation="LLFGMMIFFFSYFWVATQFQPVQIADDLKKYGGFIPGVRPGKPT
                     ADFLDFTMTRLTFAGAIFLTGIAILPMMLTQALQVPYMTAQFFGGTGLLIIVGVVLDT
                     MRQVETHLLQRHYDGFLRKGRIRSARESRARLGSGSAFSSEGLVWIYLVLAVIVIAGV
                     AVLLGRS"
BASE COUNT          720 a         1176 c         1220 g          840 t
ORIGIN      
        1 cacgatctgc gtggcttcga ggccgcgctg cgccaagcgg cgggaaccat ccttcgtgcg
       61 gatggcaacc ttgtcgcccg gcttgaggtt catcgaagga acgtcggact tgcgaccgtt
      121 gaccgcgacg tggccgtggg ccacgatctg gcgggcgccg cgcagggtct tggcgaagcc
      181 catgcggtaa acgacgttgt cgagacgggt ttcgaggagc tggagcaaga tttcaccggt
      241 gacgccgcgc ttgttgctcg caatcgcgaa ataacggcgg aactggcgtt cgaggacgcc
      301 gtattgatgg cgcagcttct gcttttcggc gagcgcggtg gcgtattcgg agagcttgcg
      361 gcgggaaccc ttcgggccat gcatgcccgg cggataactc ttgcgttcaa atgccttcgg
      421 cgagcccgag atgaacaccc cgtagcggcg gctgattttg gttttgggac ctgtgtaacg
      481 tgccatggaa ttttcgattt aagattttcg attttcgagt gatcggctca gacgcggcgc
      541 tgtttgggcg ggcggcagcc gttgtgcggc acgggagtga catcacggat gacggaaatt
      601 tccaggccga tcgcctgaat ggcgcggacg gcggattcgc ggcccgatcc cgggcccttg
      661 acgcggactt cgacttcctt gaggccgtgg cccatggcct ggcggcaggc gtcctgcgcg
      721 accatctggg cggcgtaggc cgtgcttttg cgcgaaccct tgaagccgca tttgccggcg
      781 ctggaccagc caatgacggc gccgcgctga tcggtgatgc tgacgatggt gttgttgaag
      841 gtcgcggaaa cgtgcgcgat gccgagcagg atgtttttcg cacccttggc gcggacgatc
      901 ttgaccttgt cggcgccgtc gtcgtcgagc gagaaactcg gagcggcggg cttttcgtct
      961 tccttcttgg aaccgggggc tttcttggcg gctttcggct tgacggcctt tttcgcttcg
     1021 gcctcgggag caaccggcgt gacgggcgcg gcagcagatg cgacgggcgc tacaggggcc
     1081 acgggcaaaa caggcgctac gggcgcagcc ggggccggcg tttcgggagt atcggtattt
     1141 tcagacatgg tgggttacct ccgattagac tttgccggct ttggcattcg ggttgcgggc
     1201 gacaccgacg gtcttgcgcg gtcccttgcg ggtgcgggcg ttggttccgg tgcgctggcc
     1261 acgcacgggg agtccgcggc gatggcggat gccgcggtag caattgatgg cctgaaggcg
     1321 cttgaggttc tgctgcaggt cgcggcgcag gtcgccttcg atgagaattt tgctttggtt
     1381 gatcgcctga atgatggcgt tcaattgctc cggcgaaaga tccttcgcgc ggatgttggg
     1441 atcaatgttg gcgagttcga gcactttctt ggtgttcgag gggccgatcc cgtaaatata
     1501 aggcagcgaa gcttcaatgc gcttgtcgcc gggaatatca actccgagta gtcgtggcat
     1561 aatggattat tttagatttt ggatggcgga ttttggattg cggattcgat ctggcagccg
     1621 tagcccgtga gagggcgttc caatcgaaaa tcgaaattcc aaaatcgaaa attcatgcgt
     1681 tatccctggc gctgtttatg gcgcgggtcc ttgcaaacca cgcgcaccac gtttttccgg
     1741 cgcacgatct tgcagctttc gcaaaggcgt ttgactgagg ctctgacttt catttcaaat
     1801 ttttcttagg gtgtttcgac tgattatttt tggcgatagg taatgcgtcc tttggtgagg
     1861 tcgtaaggtg acatctcgac catcaccttg tccccgggaa gcaaacgaat gaaatgcatc
     1921 cgcattttcc cggaaatatg cgcaaggacg cgacgcccgt tcgccaactc cacgcggaac
     1981 atggtattgg gtagcaactc gacaacctta ccgcttactt caatagcgtc tttctcggcc
     2041 atgtcaagac ttccggcgca tcatttgtaa tcaaaattgt gtgctcgaaa tgcgcggagg
     2101 gctggccatc ggcggagacc accgtccatt ggtcggacag catccggacc gccgccttgc
     2161 ccagattgat catgggttcg acggcgaggg tcattcccgc ctttaacttg ggccccgttc
     2221 cccgtttgcc gaagttcggc acctgcggtt cctcgtgcaa ccgccggccc actccgtggc
     2281 cgacaaattc gcggaccacc ccataacccg ccgccacggc tttttcctcg atgaaggcgc
     2341 acatgtcgcc cagccgccga ccggcgcgcg cgagcggaat cgcgtccacg agaatctgct
     2401 cggtcagggt cagcagcctt tcggtggcgg ggtcaataac gccgacgggc aggctcatcg
     2461 ccgtgtcccc aatccagccg tccctcacaa tgccgacatc cagcttgacg atgtccccgt
     2521 aattgatttt ccgcggtccg ccaatgccgt gaaccacttc ctcattgagg gaaatgcaaa
     2581 tgttcccggg aaatttgtgg tagcccagaa acgcgctgcg cacgccggct tccgccatca
     2641 atttccccgc cagccggtcg atttctccgg tcgtcacgcc gggagcgatc cggtcggcca
     2701 aagcattgag aacatgggcc gcggcctcgc ccgccttgcg cattttttcg atttccgccg
     2761 gggtcttgat tggaatcatg ccggcacccc ttcgagaatt tgggaaatca ccgcgaaaac
     2821 cgccgcgctg cccggcgccg catccacggt ccgaagccgg ccggtttggc ggtaatattc
     2881 caccacgggt tcggtcagct ggttatgctg gcgcagccgt tcgctcaatg tctccagggt
     2941 atcatcccgc cggcgctgca aaaccgcgcc gcagttcggg cattccccgc cttccctggc
     3001 cccatctccc ccattccgat acgtcgccgc gcaccgcggg caggtcaggc ggctctcgat
     3061 ccggcggcgg atttccgctt cgcccagttc gagattgatg gcgatgtcca gctctccgtg
     3121 gcgttcggcg agatcttcgt caaactttcc ggcctgcgcg agcgtgcgcg ggaatccgtc
     3181 cagcacaaaa ccacgggcgg aagcatgcgc atccagccag cgctccacga cggccaatgc
     3241 catttcgtcc gggaaatatt tcccttcccg ggtgaaggcc cgcgccgctt gtccgagcgc
     3301 ggtattttga tgctcctcct cgcgcaacag cgctccggtg gaagcgctcg gaatgccgta
     3361 tttttcggcg atgagggcgg ccagcgttcc ttttccggaa gccggcgggc ccaacagcac
     3421 aattcgggta ttcaccagaa gggttccccc gtcaggaacg gcccagcaac acggccaccc
     3481 cggcaataac aatgacggcc aggaccaggt aaatccaaac caggccctcg ctgctgaagg
     3541 cgctgcccga accgaggcgg gcgcggcttt cacgggcact gcgaatgcgg cccttgcgca
     3601 aaaatccgtc gtagtgccgc tgcaacaaat gggtttctac ctgccgcatg gtatcgagca
     3661 ccacgccgac aataatcaaa agccccgtgc cgccgaaaaa ctgcgccgtc atgtaaggca
     3721 cctgcagcgc ctgcgtcagc atcatgggca gaatggcgat ccccgtcaaa aagatggcgc
     3781 ccgcgaaggt caggcgggtc atcgtgaaat cgaggaaatc tgcggtcggc ttgccgggac
     3841 gaactcccgg gatgaatccg ccgtatttct tgaggtcatc cgcgatttgc acgggctgga
     3901 actgcgtcgc gacccagaaa tagctgaaga agaaaatcat catgccgaag agcaga
//
LOCUS       DATDAY010000034         3386 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_83021, whole genome shotgun sequence.
ACCESSION   DATDAY010000034 DATDAY010000000
VERSION     DATDAY010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 3386)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3386)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3386
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_83021"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..651
                     /locus_tag="VIS74_00745"
     CDS             <1..651
                     /locus_tag="VIS74_00745"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HEY5791792.1"
                     /translation="EILAKTENAARRIQDETAGRSGEMTLACTDGARSEHITRQLRKF
                     RRKSRGLRLIVRRVDYGSVTVPESADAFITDFPVNELPTTAVVLERAALQVAVPPKHR
                     LAEREDFAPADLIGETIITSPFDLRSPAERILLARLEAAGIAHDLELGSAHLQERFWQ
                     VSLGLGVCFCTSADRGTLDSRRISLAGAAQEAVIALIPNPNSRAAALPALVDAIRA"
     gene            complement(578..2512)
                     /gene="mnmG"
                     /locus_tag="VIS74_00750"
     CDS             complement(578..2512)
                     /gene="mnmG"
                     /locus_tag="VIS74_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006983674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HEY5791793.1"
                     /translation="MFFYPKPYDVIVIGAGHAGIEAALAAARLGCETLLLTQNADTVG
                     QMSCNPAIGGLAKGHMVREIDALGGAMGLNADATGIQFRMLNAAKGPSVRAPRAQCDK
                     KAYQFRLKARLEETPRLDLKQGNVTRLLVRDDKVAGVETSFGVTFLGSAVVVTTGTFM
                     RGLLHVGLRNQTGGRMGDTVSTFSDCLRELGFEVGRFKTGTPCRLLAASIDFSVCERQ
                     QGDEPPPRFSFLEGDLASGEEELFTLNRMKDGMFHVEQIPCWITHTNTKTHGIIRENL
                     HQSPLYAGRIEGVGPRYCPSIEDKVVKFAEKESHQLFLEPEGRHTGEIYVNGISTSLP
                     FDVQYHFIRSVRGLERAEMLRPGYAVEYDYCPPMQLERTLETKRVGGLYFAGQINGTS
                     GYEEAAAQGLMAGANAALKILGRPELVLSRSEAYIGVLIDDLVTLGTPEPYRMFTSRA
                     EYRLLLRQDNADFRLTGRGIECGLVEGERRRQFETKRLQHNGLLGRLSGWQADGVALS
                     LWFKRPENRYQLLPPTIRAEFPDSLWEVVETDLKYEGYIRRQEEQLARTSRHEHRGIP
                     AALDYLSIQGLRREAVEKLARVRPATLGQASRISGVTPADLGLLGVWLERNARTPRPE
                     SHPPGPEEPPRGSSDSESRQ"
     gene            complement(2597..3022)
                     /gene="cdd"
                     /locus_tag="VIS74_00755"
     CDS             complement(2597..3022)
                     /gene="cdd"
                     /locus_tag="VIS74_00755"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006983673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0008655 - pyrimidine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEY5791794.1"
                     /translation="MKQGWVILGFVEIERWIAAARTAREEAYAPYSKFQVGAALVAVD
                     GRIFSGCNVENISFGLTMCAERVALGQAIAAGVREFAAIAIVADTVRPVSPCGACRQV
                     LAEFHPSLEIYLCTLGGLCERFSLADLLPRPATGILDRP"
     gene            3132..>3386
                     /locus_tag="VIS74_00760"
     CDS             3132..>3386
                     /locus_tag="VIS74_00760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5791795.1"
                     /translation="MEANPPEASPAKSVIEDLDALRNDFRSALMAYASRIEEEIGLVQ
                     KSVAAQATKKKIAPAKLRDLRDMLTLLRKFSIKPEKGRRKD"
BASE COUNT          683 a         1104 c          966 g          633 t
ORIGIN      
        1 gagattctcg caaagacgga aaacgccgcg cgccgaattc aagatgagac cgctggccgt
       61 tccggcgaga tgacccttgc ttgcacggat ggcgcccgat ccgagcatat cacgcggcaa
      121 ctccgaaaat tccgacgcaa aagccgggga cttcgtctca tcgtgcgccg ggtcgattac
      181 ggttcggtga ccgttccgga gagcgcggac gctttcatca cggattttcc cgtcaatgaa
      241 ctcccaacca ccgccgtggt cctggaacga gcagccttgc aggtggccgt tcccccgaaa
      301 catcggctgg cggagcggga ggatttcgcg ccggccgatc tcatcggcga aacgatcatc
      361 acctcgccgt ttgatttgcg ctctcccgcc gaaaggatac tcctggcgcg cctcgaagcc
      421 gccggcatcg ctcacgacct ggaattgggc tccgctcacc tccaggagcg cttctggcag
      481 gtttctttgg gattgggagt ttgtttttgc acgagtgcgg atcgcgggac tctggattcc
      541 cgccggattt ccctcgccgg agccgctcag gaagccgtta ttgccttgat tccgaatccg
      601 aactcccgcg cggcggctct tccggccctg gtggatgcga ttcgggccta ggcgtccgcg
      661 catttcgctc gagccagact cccagcaacc cgaggtccgc cggcgtcacc ccgctgatcc
      721 gtgaggcttg ccccagcgtc gccggacgca cgcgagcgag tttttccacc gcttcccgac
      781 gcaaaccctg gatggagaga taatccaatg cggccggaat cccccggtgc tcatggcgac
      841 tggtccgggc aagctgttcc tcctgccgcc gaatgtaacc ttcgtatttc agatcggttt
      901 cgaccacttc ccagagggaa tccggaaact cggctcgaat cgtcggcgga agaagctggt
      961 agcggttttc cgggcgtttg aaccagagag acagggccac cccgtccgcc tgccaaccgg
     1021 ataacctgcc aagcaatccg ttgtgctgca ggcgtttggt ctcgaattga cgccgccgct
     1081 ccccctcgac cagcccgcat tcgatgcccc ggccagtaag gcgaaagtcg gcattgtcct
     1141 gacgcaacag gagacggtat tccgcccggc tggtgaacat tcgatagggt tcgggggtgc
     1201 cgagtgtgac cagatcgtcg atcagcacgc cgatgtaggc ctccgaacgg gaaagcacca
     1261 gctccggtcg gccaagaatt ttcaacgcgg cattggcccc ggccatcagg ccctgcgcgg
     1321 cagcctcttc atatccggac gtgccattga tctgtccggc aaaataaagt ccccccaccc
     1381 gtttcgtttc cagtgtccgc tccagttgca tcggcgggca gtagtcgtat tccacggcat
     1441 agccggggcg gagcatttcc gcgcgttcca acccacgcac actgcgaatg aagtggtatt
     1501 gcacgtcgaa aggcagactc gttgagatgc cattgacgta aatctccccc gtgtgccgcc
     1561 cttccggctc gagaaaaagc tgatgactct ccttttcggc aaacttgacc accttgtcct
     1621 cgatcgaagg gcaataccgg ggccccacac cttcgatgcg gcccgcgtag agcggcgact
     1681 ggtgaaggtt ttcgcgaatg atgccgtggg ttttcgtgtt ggtgtgtgtg atccagcagg
     1741 gaatttgttc cacatggaac attccgtcct tcatccggtt cagcgtaaac aactcctctt
     1801 ccccagacgc cagatcgccc tcgagaaaac taaaccgagg gggcggctcg tctccctgct
     1861 gacgttcaca aacggaaaaa tcgatcgaag cggccaggag gcggcagggg gtgcccgtct
     1921 tgaagcgtcc cacctcgaaa cccagctccc gcaggcaatc gctgaaggtg gaaaccgtgt
     1981 cccccatgcg accgccggtt tgattgcgca gaccgacgtg cagcaaacca cgcatgaagg
     2041 tccccgtcgt gaccaccacc gcgcttccca ggaaagtcac cccgaaactt gtttcgaccc
     2101 ccgcaacctt gtcgtcccga accagcagac gtgtcacgtt gccctgcttc aggtcgagcc
     2161 gcggagtttc ctcgagccgg gccttgagcc ggaactgata ggcttttttg tcgcactgtg
     2221 cgcgcggagc ccgcacgctg gggcccttgg cggcgttgag catgcggaat tgaatgcccg
     2281 tcgcgtccgc attcaacccc atcgctccgc ccagcgcatc gatctcccgg accatgtgcc
     2341 ccttggccag gccgccaatc gccggattgc aggacatctg cccgaccgtg tcggcgtttt
     2401 gggtcagcaa cagcgtttca catcccaggc gggccgccgc cagcgcggct tcgattccgg
     2461 catggccggc accgataaca atgacgtcgt agggcttggg ataaaaaaac atcggcgtga
     2521 acgggcccgc accctaccac atcgccgagg cggaaaaaga aaaaccccca gctcgctatt
     2581 gttccatgtg gaacattcag gggcggtcaa gaatcccggt cgccgggcgc gggagaaggt
     2641 cggcaaggga aaagcgctcg caaaggcccc caagcgtgca cagataaatt tccagggagg
     2701 gatgaaactc cgccagcact tgccggcacg ctccgcacgg gctgactgga cggacggtat
     2761 ccgccacgat ggcaatggcg gcaaactcgc gcacaccagc ggcaatcgcc tgccctaaag
     2821 ccacccgctc cgcgcacatc gtcagtccaa aactgatgtt ctccacgttg cagccggaaa
     2881 aaatccgtcc atccaccgcg acgagagccg cccccacctg aaatttcgag tagggcgcat
     2941 atgcttcttc gcgggccgtc cgggcggccg cgatccatcg ttcgatttcc acaaacccga
     3001 gaatcaccca tccctgcttc aagaacaagc ttcgaaggca ttggacattt tatgtctgat
     3061 attgacccgc tcctccgctc atcgagggca gccttgccac ctcgggcggc tcctgataaa
     3121 accccgaaca gatggaagcc aatcctcccg aggcatcgcc cgcgaaatcg gtgatcgagg
     3181 atctcgacgc gctgcggaat gacttccgct ccgcgctgat ggcctacgcg agccgcatcg
     3241 aagaggaaat cggcctcgtg caaaaatcgg ttgccgcgca ggcgacgaaa aaaaagatcg
     3301 ccccggcgaa gctgcgcgat ctgcgcgaca tgctcacgct gctgcggaag ttttcgatca
     3361 agcccgagaa aggccgccgc aaggat
//
LOCUS       DATDAY010000035         2441 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_2135315, whole genome shotgun sequence.
ACCESSION   DATDAY010000035 DATDAY010000000
VERSION     DATDAY010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2441)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2441)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2441
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2135315"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..322
                     /locus_tag="VIS74_00765"
     CDS             <1..322
                     /locus_tag="VIS74_00765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5791796.1"
                     /translation="HTYASCAELDLEVLSETDQEALEFAWNQFGNIEKFQLADLTHRY
                     PEWKRHEAALIAKSVSRVPMAYEDFLLDPEENLEPCHPLSEEEKQDRRELLRETAAFE
                     ARWR"
     gene            329..754
                     /locus_tag="VIS74_00770"
     CDS             329..754
                     /locus_tag="VIS74_00770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5791797.1"
                     /translation="MSGAVPFQIRLTLRTGSVYYFADRALHSAEPHFFIVVNRSPLSD
                     ELLILAVVTSQVDKVRRFRKSLPDTLVALDPVLYAELSKPSIVDCNQVFPKTLKEFTE
                     LFEREEIRHHQDLPGDLLALIRNAIHASPIVTPECKKLI"
     gene            839..>2441
                     /locus_tag="VIS74_00775"
     CDS             839..>2441
                     /locus_tag="VIS74_00775"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF008220.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008736 - L-fucose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019317 - fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HEY5791798.1"
                     /translation="MKNPVLENLPKVGIRPTIDGRLGGVRESLEAQTMGMAQSVAKLI
                     SDSLRTPNGDPVECVIADSCIGGVAEAVACAEKFRRENVGVSLTVTPCWCYGSETMDA
                     DPLIPKAIWGFNGTERPGAVYLAAVLAGHTQKGLPAFGIYGRDVQEATDTAIPEDVQE
                     KILRFIRAGLAVATMRGRAYLGMGGTSMGIAGSILDPSLFEQYLGMRVESVDMTEFIR
                     RIERGIYDKAEFAKALKWAKANCREGKDWNGPGKKRSRQQLDAEWEISIKMALIARDL
                     MIGNPKLKKLGYGEEAQGHYAIASGFQGQRQWTDHMPNGDFMEAMLSTSFDWNGRRSP
                     FVVATENDALNGVTMLFGYLLSHTAQIFADLRTFWSPEAVQRVTGHTLEGAAAGGFLH
                     LINSGPAALDGSGRQTRGGQPALKPFWEITNAEVKACLEATSWHPSITEYFPGGGWST
                     RYVTRGGMKATIFRLNMVEGLGPALQIAEGETIELPDKAHELLEERTNPTWPTTWFSP
                     YTTGRGAFRDTYSVMNNWGANHCVMS"
BASE COUNT          519 a          726 c          729 g          467 t
ORIGIN      
        1 gcatacttat gccagctgcg ctgaactgga tttggaagtc ctttcggaaa cggatcagga
       61 ggcgttggaa tttgcgtgga accaattcgg taatattgag aaatttcaat tagccgattt
      121 gacgcaccgc tatcccgaat ggaaacggca tgaggcggca ctaatcgcca aaagcgtttc
      181 acgtgtgccc atggcgtatg aggattttct gctcgatccc gaagaaaacc tggaaccctg
      241 ccatccgctt tcggaggaag aaaagcagga tcgaagagaa ttgcttaggg aaacggcggc
      301 atttgaagca cgctggcgct gacggcaaat gtccggagcg gtgccattcc aaatacgtct
      361 gacattgcgg actggcagcg tttactactt cgcggatcgt gcccttcact ctgcagagcc
      421 gcattttttc attgtcgtca accggagccc cctcagtgac gaactgctga ttcttgccgt
      481 ggttacttct caggtggata aggtaaggcg attccgcaaa tcgcttccgg acacgctggt
      541 ggccttggat cccgtattgt atgctgaact cagcaagcct tcgatcgtgg attgtaatca
      601 ggttttccca aaaacgctca aggaattcac cgagcttttt gagcgggagg aaattcgcca
      661 tcatcaggat ttgccaggcg atcttctggc gctgattcga aatgccattc atgcaagtcc
      721 aatcgtcaca cctgaatgca aaaaattaat ctgactcttc gggatcaatg aaactgcttg
      781 aacccccggc tgatcgaaga ataagacaaa agatcggcga tccgcctcct ccccgtttat
      841 gaaaaacccc gtcctcgaaa atctcccgaa ggttggcatt cgtccgacca ttgatggccg
      901 tctcggcggc gtgcgcgaat cgctggaagc ccagaccatg ggcatggccc aaagcgtggc
      961 gaaattgatc agcgactccc tccgcacgcc caatggcgat ccggtggaat gcgtgattgc
     1021 ggactcctgc atcggcggcg tggccgaggc ggtggcctgc gcggaaaaat tccgccgcga
     1081 gaatgtcggc gtctcgctca cggtgacgcc gtgctggtgt tacggcagcg aaacgatgga
     1141 cgcggatccc ttgattccca aagccatttg gggctttaac gggaccgagc ggccgggcgc
     1201 ggtttatctc gccgccgtgc tcgccgggca cacgcagaag ggcctgcccg cgttcggcat
     1261 atacgggcgc gacgtgcagg aggccacgga cacggcgatc ccggaggacg tgcaggaaaa
     1321 aatcctgcgc ttcatccgcg ccggcctcgc ggttgccacg atgcgcggac gcgcgtatct
     1381 cggcatgggc ggcacttcga tgggcatcgc cggttcgatc ctggatccgt cgctctttga
     1441 gcaatacctc ggcatgcgcg tggagtcggt ggacatgacc gaattcatcc gccgcatcga
     1501 gcgcggcatc tacgacaagg cggaattcgc caaggcgctc aaatgggcga aggcgaactg
     1561 ccgggagggc aaggattgga acggccccgg aaaaaagcgc agccgccagc agctcgacgc
     1621 ggaatgggaa atctccatca agatggcgct catcgcccgc gatctcatga tcggcaatcc
     1681 gaagctgaag aaactcggct acggcgagga agcccagggc cattacgcaa tcgcttccgg
     1741 cttccagggc cagcgccagt ggaccgacca catgccgaac ggcgatttta tggaagcgat
     1801 gctctccacg tcgttcgact ggaacggccg ccgttcgccg tttgtcgtcg cgacggaaaa
     1861 tgacgcgctc aacggcgtga cgatgctttt tggctacctg ctctcccaca ccgcgcagat
     1921 ttttgccgac ctgcggacgt tctggagtcc ggaggcggtc cagcgcgtca ccggccacac
     1981 gctcgaaggc gcggccgcgg gcgggttcct gcacctcatc aattccggcc ccgcggcgct
     2041 cgacggctcg ggccgccaga cccgcggcgg ccagccggcg ctcaagccgt tctgggaaat
     2101 cacgaacgcg gaagtgaagg cctgcctgga ggccacgagc tggcatccct ccattaccga
     2161 atatttcccc ggcggcggct ggagcacgcg ttacgtgacc cgcggcggca tgaaggccac
     2221 catcttccga ttgaacatgg tggagggcct cgggcccgcc ctgcaaattg ccgaaggcga
     2281 aacgatcgag cttcccgaca aggcgcacga gctgctggag gagcgcacga acccgacctg
     2341 gccgaccacg tggttttccc cctacaccac cggcaggggc gccttccgtg acacgtattc
     2401 cgtgatgaac aactggggcg caaaccactg cgtgatgagc t
//
LOCUS       DATDAY010000036         7439 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_1665624, whole genome shotgun sequence.
ACCESSION   DATDAY010000036 DATDAY010000000
VERSION     DATDAY010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 7439)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7439)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7439
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1665624"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1427)
                     /gene="ccsA"
                     /locus_tag="VIS74_00780"
     CDS             complement(<1..1427)
                     /gene="ccsA"
                     /locus_tag="VIS74_00780"
                     /inference="COORDINATES: protein motif:HMM:NF013725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HEY5791799.1"
                     /translation="MKLSAGVDYTALDALPVQHEGRRKPFLAFAQENILLLSGKTAVA
                     AEGRKLAAGELVTRLWLEPEKWLDHPVILVDYLPLKEAAGLAPDRKWFSYTELMANPG
                     LQAFFANAAVPGRKLEGLPKKAEEVHRRLRTFEALVNGSLYRVAPLPAGNDWVALPAH
                     QPEYAALRNAHESENAAEYLHAAQTLQHLLGALAPEKQPPAWKIGLEVFYQKLHPFRW
                     AWIFYAASAILLAVTWKYGRAPGYRIAWALVLAGFAFQVFGFVSRILIAGRPPVTNMY
                     ESVIWVSFGAMAFALIFEAIYRSRFFILGAVPVTVFALILADSQPAALNPAISPLVAV
                     LQSNFWLTTHVLTITLGYAAFALALGVAHLALGQVIFGRKLSPELADYVYRAMQVGVL
                     LLATGTVLGAVWANYSWGRFWDWDPKETWALVALLVYLFILHGRLAGRWAGFGLAVGA
                     VLAFQSVIMAWYGVNFVLGVGLHSYG"
     gene            complement(1496..3214)
                     /locus_tag="VIS74_00785"
     CDS             complement(1496..3214)
                     /locus_tag="VIS74_00785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5791800.1"
                     /translation="MIRKTTPLGRLIYFLGSLNFALILLGTIAIACAVATIFESRFDT
                     KVAQTYIYKAPWFVFWLGFLCVNLFCVTLTRWPWQKKHLGFVITHYGIITLLIGAVIG
                     SKFGFEGNVTLHAGEPPANRLTTARSVIQLEGPMDRALYTLPFDAAAVRPSEKRPKRF
                     GVPGTKWRIVADNYAPDLVRDPRLVKTEGGRPGLILKMDSRMAAQQLGIPLLLHDPEL
                     GSADLFGLAKIQWMESLPAHEPKRERETQVVFGNYAPIVQSVGGGSGIPIHLSKDGAE
                     LTIAQETYRRAEVMDRELTVGSAKVRPSGYWPDFVMQNGRPATQSQEPRNPAVMLQVD
                     YEAAPPARGLALELAPGRGGVNYQFVRDGRIVSRGAASAGEEFVSGWMDWRVRVEEYE
                     PSAALRFETRLPREGESKTGIPGFWARLETEDGARGEPRWVESGRLTELAAGEARVRI
                     GYGLEIIKVSFNLKLLNFEVPRYEGTETPSDYISTLEFTDLRTGQSRMGVAKMNHPAS
                     WPGGFWAVTTGLNYKFSQAEWNPRDLGETTLQVLYDPGWLLKWTGSLAICIGIALMFY
                     WRPKKA"
     gene            complement(3211..5091)
                     /locus_tag="VIS74_00790"
     CDS             complement(3211..5091)
                     /locus_tag="VIS74_00790"
                     /inference="COORDINATES: protein motif:HMM:TIGR01525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEY5791801.1"
                     /translation="MPSCCHCRAEPAAPDFRPAREWMRMGLAALVAAQSMIFSLAINL
                     SPPSGMARTVLHAALAVSAVAVFALLGGPVLRAAWQPRIVVEQLFLAGIFGAFAASVY
                     CSLTGVGHVYYEVVAVLLAIDTLGRALGERRRSEVLAAAEQLDREFDPPAPAGGVVEI
                     AAGGPVTVDGVILEGAALVRETALTGEPFPVVKRPGDPVLAGSRSLDGALRVRPFAGE
                     RKLDELLRAVRDARRRPARLQREADRLAAWFLPAVMGIALAVFAIGGLRGEWMPATFN
                     ALAVILVACPCSLGLATPIGLWSALAELAQRGILARSGDLVEKLARVNAVIFDKTGTL
                     GEEAMQLVDFVALGDREALLREVSALQRASDHPIAAAFAPSGEALEVSELELLPGVGL
                     RGRVGGHLLEIGNSRLAGDIEPLRRLLREESHLVVIRRDGALAGLASLRERLRATSLR
                     AIAELEAAGLACRIFTGDQTAPGLERAETGMSPLQKRDRVAALQRTGERILFVGDGMN
                     DAPALAQADAALAVASGSALARESADGEILGGNLLAVPEAIALCRRTLRVIRGNLYFA
                     AGYNLIGISLAAAGLLHPVAAALLMLASSFTVSWRALRASPPAAPMRRNMIEPPFREA
                     LA"
     gene            5174..6514
                     /locus_tag="VIS74_00795"
     CDS             5174..6514
                     /locus_tag="VIS74_00795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5791802.1"
                     /translation="MAKRIFWISLGTLAVLAAAAFLIGYAWIGRYLRSEPFRQLASAK
                     TSGALKVEGAFQPLSWTGASVYSDRFQGAAAPRGYLEKLDANRIRVEVNFRKILNGAW
                     RVDEVNIGHLAATFGAAESLQPAPSAPAEPPASALPGWLPRRFEIGPVNVENADLAAR
                     DLALNGAKLTLRPDGTAWVLHGSGGKFRAKSFPELSLHSLTARVTASQLFLTQAAARP
                     ASGGNLTATGEMAFASGESEFRLDFTGIQAREVLPPEIAHYLSGLLGGTAILQTAAGS
                     GSTSGNFVLTEGMVKDLPVLQQLAKFTQSPQFERLPLQTMSGDFLDDAKGLDVKNFVA
                     ESKGLLRVEGQFHIGNHQELRGHFQVGLTPQTLQWLPGSRERVFTAARDGYLWTAVNL
                     GGTTAAPSEDLSDRLAVAMGQQVIQQGADLLKNSPDKAIDTIKDAVNILAPFTR"
     gene            6598..7293
                     /locus_tag="VIS74_00800"
     CDS             6598..7293
                     /locus_tag="VIS74_00800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5791803.1"
                     /translation="MIHANCRVRLTADDFDFIVRALSTKSSDAVSLPRLLTDESTRDE
                     LLDHEVLTAAILESPETLRISPRLLFYVLCRKVLRKTAVRSRESADYIASLLERFQNS
                     ARLDSPDGLSERQLRYLSDMLLALSQAGPREAFILRAHIGNYSLFLSGMFADSLEKCR
                     RKGGPGLSFYEQVGRSSFQAAAGHREAQRFQLDKIYRELADGFHEARLALNDLARRLL
                     HLDTPPAPIIAIG"
BASE COUNT         1374 a         2566 c         2306 g         1193 t
ORIGIN      
        1 ccgtagctgt gcaggccgac gccgagcacg aaattcacgc cataccaggc catgatgacg
       61 gattggaacg ccagcaccgc gccgactgcc agcccgaatc ccgcccaacg tccggcgagg
      121 cggccgtgca ggatgaataa atagaccagc agcgccacca gcgcccaggt ttccttcgga
      181 tcccaatccc agaagcggcc ccaggaataa ttcgcccaga ccgcgccgag cacggtgccc
      241 gtggccagca gcagcacgcc cacctgcatg gcgcgataca cgtaatccgc cagctccggg
      301 gaaagtttcc ggccaaaaat cacctgcccg agcgcgaggt gcgcgacgcc cagcgccagg
      361 gcgaaggcgg cgtaacccag ggtgatggtc agcacgtgcg tggtcagcca gaaattgctc
      421 tgcagcaccg cgacgagcgg cgagatggcc ggattcagcg ccgccggctg gctgtcggcg
      481 agaatcaagg cgaacaccgt caccggaacg gcgccgagga tgaaaaaccg ggagcggtag
      541 atggcttcga agatcagcgc gaacgccatg gcgccaaagg aaacccagat caccgattca
      601 tacatgttcg tcaccggcgg acgcccggcg atgagaatgc ggctcacaaa tccgaacacc
      661 tgaaaggcaa acccggcgag caccagcgcc caggcgatcc ggtaacccgg cgcgcggccg
      721 tatttccacg tgaccgcgag cagaatggcg ctcgccgcgt agaaaatcca cgcccagcgg
      781 aaggggtgca gcttttgata aaatacttcg agcccgatct tccaggccgg cggctgtttc
      841 tcgggcgcga gggcgccgag caggtgctgc agggtttgcg cggcgtggag atactccgcg
      901 gcgttttccg attcgtgggc gttgcgcagc gcggcgtatt ccggttgatg ggccggcagc
      961 gccacccagt cgttgcccgc gggcaggggc gccacgcggt agagcgagcc attgacgaga
     1021 gcctcaaagg tccggagccg ccggtggacc tcctcggcct tcttcggcag gccttcgagt
     1081 ttgcggcccg gcacggcggc gttcgcaaag aatgcctgca agccgggatt ggccatcagc
     1141 tcggtgtaac taaaccactt ccggtccggc gccagccccg cggcctcctt gagcggaagg
     1201 tagtccacca ggatgaccgg gtgatccagc catttttcgg gttcgagcca caggcgcgtg
     1261 accagctcgc cggcggcgag cttccggcct tcggccgcca cggcggtttt gccggacagc
     1321 agcaggatgt tttcctgcgc gaaggccaga aagggcttcc tgcggccctc gtgctggacc
     1381 ggcagggcgt cgagggcggt gtagtccacg ccggcggaga gcttcaaaaa ggggactaat
     1441 aggagcaata ggacccatgc gccgatccgc gttttgcgct ttgtgctttg cttcgtcatg
     1501 cttttttcgg cctccagtaa aacatcaggg cgatgccgat gcaaatcgcc agagagccgg
     1561 tccatttcaa aagccagccg ggatcataca aaacctgcag cgtggtttcg cccagatcgc
     1621 gggggttcca ttcggcctgg gaaaatttgt agttaaggcc ggtcgtcacg gcccagaagc
     1681 cgccgggcca gctcgcggga tggttcattt tcgccacgcc catccggctc tgcccggtgc
     1741 ggaggtcggt aaattccagg gtcgagatgt agtcggacgg ggtttcggtg ccctcatacc
     1801 gcggcacttc aaaattcagc aatttcaaat taaacgacac cttgatgatt tccagcccgt
     1861 agccgatccg gacgcgggcc tccccggcgg ccagttccgt gagccggccg gattccaccc
     1921 agcggggttc gccgcgggcg ccatcctccg tctccaaccg cgcccaaaag cccgggatgc
     1981 cggtcttcga ttcgccctcc cgcggcagcc gggtctcaaa acgcagcgcg gcggacggct
     2041 cgtattcctc cacccggacc cgccaatcca tccagcccga gacaaattcc tctcccgcgg
     2101 acgcggcgcc ccgggagacg atgcggccgt cgcgaacgaa ttgataattc acgccgcccc
     2161 gccccggcgc cagttccaag gccaagccgc gcgcgggcgg cgcggcttcg tagtccacct
     2221 ggagcatcac cgccggattc cgcggctcct ggctttgggt ggcgggccgt ccattttgca
     2281 tcacgaaatc cggccaataa cccgatgggc gaaccttggc ggaaccgacg gtcagttcgc
     2341 gatccataac ctccgcgcgg cgataggttt cctgcgcaat ggtgagttcc gccccatcct
     2401 tgctcagatg gatcggaatg ccgctgccgc cgcccacgga ctgaacgatc ggcgcgtaat
     2461 ttccaaacac cacctgcgtc tcgcgttccc gcttgggctc atgcgcgggc agggattcca
     2521 tccactggat ttttgccagg ccgaagagat ccgcgctgcc caattccggg tcgtgcagca
     2581 gcagcggaat gcccaattgc tgcgccgcca tgcggctgtc cattttcaga atgagccccg
     2641 ggcgcccgcc ttccgtcttc accaaacgcg gatcgcgaac caaatccggc gcgtaattgt
     2701 cggcgacgat ccgccatttc gtgcccggca cgccgaaccg cttcggacgc ttctccgagg
     2761 gccgcaccgc cgccgcgtcg aagggcagcg tgtaaagcgc gcgatccatc gggccctcca
     2821 attggatcac gctgcgcgcg gtcgtgaggc ggttcgccgg cggctccccc gcatgcagcg
     2881 tgacattgcc ttcgaacccg aatttggagc cgatgaccgc cccgatcagc agcgtgatga
     2941 tgccgtaatg cgtgatgaca aatccgagat gtttcttctg ccacggccag cgcgtgagcg
     3001 tgacgcagaa aagattcacg cacaaaaacc cgagccagaa cacgaaccac ggcgccttgt
     3061 aaatgtaggt ttgcgcgacc ttggtgtcga agcgcgattc gaaaatcgtc gccactgcgc
     3121 aggcaatggc gatcgtcccc agcagaatca gcgcgaagtt cagcgacccc aaaaaataaa
     3181 tgaggcgccc gagcggcgtg gttttccgga tcacgcgagg gcttcccgga agggaggttc
     3241 gatcatgttg cgccgcattg gtgcggcggg cggcgaagcg cgcagtgccc gccagctgac
     3301 ggtaaaactc gacgccagca tgagcagcgc cgcggccacg ggatgcagca ggccagccgc
     3361 cgcgagggaa atgccgatga gattgtagcc cgccgcgaag tagagattcc cgcgaatgac
     3421 gcgcagggtc cggcggcaga gggcgatcgc ctcgggaacc gccagcagat tgccgccgag
     3481 gatttcgcca tccgccgact cgcgcgccag ggcgctgccg ctggccacgg ccagcgcggc
     3541 gtccgcctga gccagcgcgg gggcgtcgtt catgccatcg cccacaaaga gaatccgctc
     3601 ccccgtccgc tgcaaggccg cgacccggtc ccgtttttgc aggggggaca tccccgtttc
     3661 cgcccgctcc aatccgggcg cggtttggtc gccggtaaaa atccggcacg ccaggccggc
     3721 ggcttcgagt tcggcgattg cgcgcagcga cgtggcccgc agccgctccc gcaggctcgc
     3781 caggccggcc agcgcgccgt cgcgccggat gaccacgaga tgcgattcct cccgaagcaa
     3841 acggcgcagc ggttcaatgt cacccgccag ccgcgaattg ccgatttcga gcaaatgtcc
     3901 gcccacgcgg ccgcgcaggc cgacgccggg gagcagttcc aattcggaaa cttccagagc
     3961 ttcgccggaa ggcgcaaacg cggcggcgat cgggtgatcg ctcgcccgct gcaaggccga
     4021 aacctcgcgc agcagcgcct cgcggtcgcc cagcgccacg aagtccacga gttgcatcgc
     4081 ctcctcgccg agcgttccgg ttttgtcgaa aatcaccgca ttcacccgcg cgagcttttc
     4141 gaccagatcc ccggaacgcg ccaggattcc ccgctgcgcg agttccgcca gcgcgctcca
     4201 gaggccgatc ggcgtggcca ggccgagcga acacgggcag gcgacgagaa tgaccgccag
     4261 cgcgttgaac gtcgccggca tccactcgcc gcgcaggccg ccgattgcaa aaaccgcgag
     4321 cgcgattccc atgaccgccg gcagaaacca ggcggcgagg cgatccgcct cgcgctgaag
     4381 ccgcgccggc cggcggcggg cgtcccgaac cgcgcgcagc agctcatcca gcttccgctc
     4441 gccggcgaag ggccgcacgc gcagcgcgcc atccaggctg cggctgcccg cgagcaccgg
     4501 gtcgccgggc cgcttgacca ccggaaacgg ctcgccggtc agcgccgttt cccgcaccag
     4561 cgcggcgcct tcgaggatca cgccatccac ggtcaccggg ccgcccgcgg cgatttccac
     4621 cacgccgccc gccggcgccg gcggatcaaa ctcgcggtcc agttgctccg ccgccgcgag
     4681 cacctcgctc cggcgccgtt cgccgagcgc ccggcccaac gtgtcaatcg cgagcagcac
     4741 cgccaccacc tcgtaataga catgccccac gccggtcagg ctgcaataaa ccgacgcggc
     4801 aaaggcgcca aaaatgcccg ccagaaaaag ctgctccacg acgatccgcg gctgccaggc
     4861 cgcgcgcagg accgggccgc cgagcagcgc gaagacggcc accgccgaaa ccgccagcgc
     4921 ggcgtgcagg acggtccgcg ccattcccga cggcggcgag agattgatgg cgagcgaaaa
     4981 aatcatggac tgcgccgcga cgagcgccgc cagccccatt cgcatccatt cgcgagcggg
     5041 gcggaagtcc ggggccgccg gttcggcccg gcaatgacag caactcggca tcgcgaacac
     5101 ggtattggtt caccccgcaa ttgcaacagg agaaggtttg aggcaaattg acgcacctca
     5161 tccctattcc ttcatggcca aacgcatttt ctggatttcc ctcggcaccc tcgccgtcct
     5221 ggcggccgcc gcttttctga tcggctatgc ctggatcggc cggtatctcc gcagcgagcc
     5281 tttccgccag ctggccagcg ccaaaacgag cggcgcgctc aaggtggagg gagccttcca
     5341 accactgagc tggaccggcg cctcggtgta ttccgaccgg tttcagggcg cggcggcgcc
     5401 caggggctat ctcgaaaaac ttgacgccaa ccgcatccgc gtcgaggtca acttccgcaa
     5461 aatccttaac ggcgcctggc gcgtggacga agtgaacatc ggccatctcg ccgccacctt
     5521 cggggcggcg gaatcgctcc agcccgcgcc ctccgccccg gcggagccgc ccgcctccgc
     5581 cctgcccggc tggctgccgc ggcgttttga aatcgggccg gtaaacgtcg aaaacgccga
     5641 cctcgccgcc agggatctgg cgctcaacgg cgcgaagctg accctccgcc cggatggcac
     5701 cgcgtgggtg ctccacggtt ccggcggcaa attccgcgcg aagagctttc ccgagctttc
     5761 gctccattcg ctgaccgccc gcgtcacggc ctcgcagctt ttcctgaccc aggccgcggc
     5821 ccgtcccgct tccggcggca acctcaccgc caccggcgaa atggccttcg ccagcggcga
     5881 atccgagttc cgcctggatt tcaccggcat tcaggcccgg gaagtcctgc cgcccgaaat
     5941 cgcccattat ctgagcggcc tgctcggcgg gacggcgatc ctccaaaccg ccgccggttc
     6001 tggcagcacc tccggaaatt ttgtgctcac ggagggaatg gtcaaagacc ttcccgtcct
     6061 gcagcagctc gccaaattta cgcaatcccc gcagttcgaa cggctgccgc tgcaaaccat
     6121 gtcgggcgat tttctcgacg acgcgaaagg actcgatgtc aaaaatttcg tggccgaatc
     6181 caagggcctg ctgcgcgtgg agggccagtt ccacatcggc aaccaccagg aactgcgcgg
     6241 ccatttccag gtcggcctca ctccgcaaac gttgcaatgg ctgcccggct cgcgggaacg
     6301 ggttttcacc gccgcgcgcg acggttacct ttggaccgcc gtcaatctcg gcggcaccac
     6361 ggccgccccc agcgaggatc tgtcggaccg gctcgcagtc gccatgggcc agcaggtcat
     6421 ccagcaaggc gcggacctcc tgaaaaacag tccggacaag gcgattgata cgatcaagga
     6481 cgcggtgaac atcctcgcgc cgtttactcg gtagggcgaa gttttaagcg gccgaatccg
     6541 cttcttaaaa cggcaaactt tccgcttggc acatttcgtg ctagtcatga gctcccgatg
     6601 atccacgcga attgccgggt tcgattgacg gcggacgact tcgattttat cgtccgggca
     6661 ctttccacca aatcgtccga cgcggtctcg ttgccgcgat tattgacgga cgaatccacc
     6721 cgcgacgaat tgctggatca cgaggtcttg acggcggcga ttttggagtc gcccgagacg
     6781 ctgcgcattt cgccgcggct gctgttttac gtcctgtgcc ggaaagtgct gcggaaaacc
     6841 gcggtgcgca gccgggaaag cgcggattac attgcctcgc tgctggagcg gttccaaaat
     6901 agcgcgcggc tggactcgcc ggacggactg tccgagcggc aattgcgcta cctctccgac
     6961 atgctcctgg cgctcagcca ggccggcccg cgcgaggcgt tcatcctgcg ggcgcacatc
     7021 ggcaattatt cgctctttct cagcggcatg tttgcggaca gcctcgaaaa gtgccgccgc
     7081 aagggcgggc cgggcctgtc gttttacgaa caggtcgggc gttcgagttt tcaggccgcg
     7141 gccggccatc gcgaggcgca aaggttccag ctcgataaaa tttaccgcga actcgcggac
     7201 ggctttcacg aagcgcgcct cgccctcaac gacctggccc gccggctgct ccacctcgac
     7261 acgccgccgg cgccaatcat cgcgattgga tgaacacgct tttctccgac atccagaatt
     7321 tgttcgagcg cacgtatgcg agcgtgggca tcaatctcga ggagtgcctc atcgacggcc
     7381 cgcgctgccg caagctcacc gcgctcgccg gcgcgcaggc cgcggagttg agcgccatg
//
LOCUS       DATDAY010000037         5863 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_1494147, whole genome shotgun sequence.
ACCESSION   DATDAY010000037 DATDAY010000000
VERSION     DATDAY010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 5863)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5863)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5863
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1494147"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            33..>5863
                     /locus_tag="VIS74_00805"
     CDS             33..>5863
                     /locus_tag="VIS74_00805"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG-like jellyroll fold domain-containing
                     protein"
                     /protein_id="HEY5791804.1"
                     /translation="MRRWSLAIALLWAGGALFAADPPAWWSASETRILDANATPSNYS
                     PVLAGQLKHAAKQAKKYLDANVVGGAGPAINTMVAGFTPQSGVAYTPEQLAVIQKANY
                     TPVNLGQLKAVAKPFYDRLKGAGYNAKQNLIDRGYASNWSYDYPWNPNTPAAQNYKIA
                     NLGQLKMAFSFDLRNLDFDSDGIPDWWEQKYFSSSGLDGQGDPDGDELENLEEYQQGS
                     NPTDYYNGKTPVVAIIDGNGQSGSVSSFLVKPLVIKVTNSAGNALVGAPVTFQVSQGN
                     GKLATSAVGTPDLASTLSLRTDAGGKVSVYFKGGESEGKAVLLATAATATQQAQCVFT
                     ETILPGADLKLWLKADALAQSNGTGMAQWSDQSGNGNHAIQATAAKQPKYQLNVLNGK
                     PVVRFDGSDDQLAVNRVVQDDLTMVVVFKSSQGLGTGTQWGEGAGLVDAAVNGLNNDF
                     GLSLNAQGRVLGGVGSSINDTSVYSGAGYNDGKAHVAIYRRVKGTGELKLYVDGVLQP
                     AAAGGTHSLTAPTRMTIGSIQTNQNYLSGDIAEVLVYGRALADHELQWVEFHLGNKYN
                     LAAVANQDWNEFFDAWEMKYFGHTGVNAAEDGDGDEKSNLDEYQEGSDPTDYYNGQTP
                     GVAALGGNNQSGPLSAFLTYPFTVKVSDSAGNPLKNAPVTFTMSQGAGKLALANTAES
                     ALVDTLSLRTDGQGLAQVYYRGADQAEVAAIQAVAGTAPLQAQALFTAGTVPADVLGG
                     GLKLWLKADAVAQSSGTGVAQWSDQSGNNHHAIQATAAKQPKYQLNVLNGKPVVRFDG
                     IDDQLAVTRVVQDDFTIAVLFKSSQGVGIQTKWWAGAGLVDAEVGGPRDDFGLSLNAN
                     GQVLGGTGGENLADTFLYSGTGYNNGQGRVAVFRRVKATGALELYVDGILQATAAGGT
                     QPLDEAQWMMIGSIQTSMNYLQGDIAEVVVYEQALMPEDQRRLEIYLGDKYSLPALRD
                     TDDNGLPDQWEDDHFEQLGVNPNADADGDGLTNLEEFQQSGDPRDYYRQGRITITPLL
                     AVVGGNNQSGRASDFLASELEVSVVNAAGGAPLRNAPVTFKVTQGDAKLAPSGAASPL
                     VAQKLVRTGADGHARVRVKLGTAFDSRIQAQTGTAEPVIFSALGWKLLGQWTFEKDSA
                     GAAYDVSGQQNHATKEEWAGLSASGGALSFSGTSGYLQTPVANQAQGALMAWIYPRVS
                     TAAPAFQSIFDSDVYGQFGTGWGLNNGMITVLLDNLVWNTGVPVEYDKWQHVALVYDA
                     SSAKLYVNGLVKATCTYTQGAVTPANYRIGRSNAYAHYFDGKLDDVRIYSGTLLAMDV
                     ALISGGDADGDGFSNAQEVLLGTNYQAADTDGDGLSDGEEAGFRIPLSTGTDPTKADS
                     DGNGINDGQEFLKVRVGISDYDLYDDIWDVYVNGIWVVRNVATFKFMESKPAYKTIFL
                     KKGTTAKFTLGRPDLNQLGSSDEYHLRFENPAGAELPIAWQAAPGNSPLISAFYYNGD
                     TPPDPSALKWNFYISDSNDGDADGLDANKENSLGTDPADPDTDHDGMPDGWEVDHSLN
                     PRSPNDAPRDADGDGVTNLEEYRYEGDPHDGDADGDGLPDLWEMKEGAFLASDDAAKA
                     SYHHNGLRHLEQFSKPATDTDGDGLSDYAEGLWQTQPGVPDTDGDGMPDGYEVANGLN
                     PKDGQDALADKDNDGVANLAEYQAGTLGWDYDTDNDGLSDGWERQYGTPAKSSMEGLQ
                     AWWRMDHGPGYPLYDTSGNRHDGEWHGDASLLQRLEPEIWAGNYPYRSVSAWDRSALV
                     LRGAHSFVTVPEGEGLVPSGSCTWAFEVNPDDVARERTTTLFEKEGSYAVRLGSDRKL
                     EVELVGKTGVRRKFFTAGALAAGQWTSVGIVLDAGNPPKVRLYLNGVMNSEASLGEAE
                     LGKTDRALILGSARIEPPMAPQAQLDDLRFYGRTLSAAELAGL"
BASE COUNT         1249 a         1489 c         2067 g         1058 t
ORIGIN      
        1 attgattggg agcggcggat ccgggattta taatgagacg ctggagcctg gcgattgctt
       61 tgttgtgggc agggggagcc ctgttcgccg ccgacccgcc ggcgtggtgg agcgcgtccg
      121 agacgcgcat tcttgatgcg aatgcgactc ccagcaatta cagtcccgtg ctggccggcc
      181 agctcaagca tgcggccaag caggccaaaa aatacctcga tgccaacgtg gtgggtggag
      241 cggggccggc catcaacacg atggtcgcgg gatttacccc gcagagcggg gttgcctata
      301 ctcccgagca actagccgtt atccagaagg ccaattatac ccctgtgaat ctcggccagc
      361 tcaaggcggt ggccaagccg ttttatgacc ggctcaaggg ggcgggctat aacgcgaaac
      421 agaacctaat tgatcgagga tatgcctcga attggagtta cgattatcca tggaatccaa
      481 acacgccggc cgcgcagaac tataagatcg ccaatcttgg gcaattgaag atggccttca
      541 gttttgatct gaggaatctc gatttcgaca gtgatggcat ccccgactgg tgggaacaaa
      601 aatattttag ctcctccggg ctggatggcc agggtgatcc ggacggggac gagttggaga
      661 atctggaaga ataccaacaa ggtagcaatc cgacggatta ttacaacggg aaaacaccgg
      721 tggtggcgat tattgacggg aacgggcaga gcgggtccgt ctcgagtttt ttggtcaagc
      781 ctctggtaat aaaggttacg aatagcgcgg gaaatgcgct ggttggtgcg ccggtgacct
      841 tccaggtcag ccaggggaat gggaaactgg cgacctccgc cgtgggaacg ccggatttgg
      901 caagcacctt aagcctgagg acggacgccg gtggaaaagt gagcgtctat ttcaagggag
      961 gggaaagcga ggggaaggcc gtccttctcg ccacggcggc gaccgcgacg caacaggcgc
     1021 aatgcgtgtt tacggaaacg atcttgcccg gcgccgacct caaattgtgg ctcaaagcgg
     1081 atgcattggc tcaatcgaat gggacgggga tggcgcagtg gagcgatcaa tccggcaatg
     1141 gaaaccatgc cattcaggcg acggcggcga aacagcccaa atatcaactc aatgtcctga
     1201 atggaaagcc ggtggtgcgg tttgacggga gcgatgacca actggcggtg aaccgggtgg
     1261 tgcaggatga tttgacgatg gtcgtggtgt tcaagagcag ccagggcttg ggcacgggga
     1321 cccaatgggg ggaaggagcg ggcttggtgg acgccgcggt caacggcctg aacaacgatt
     1381 tcgggttgag cctcaatgcg caggggcggg tgctgggtgg cgtgggaagc tccattaatg
     1441 acacctccgt gtattccgga gcgggttata acgacgggaa ggcgcacgtg gcgatttatc
     1501 gccgcgtgaa ggggacgggc gaactgaagc tttacgtgga cggggttctc caaccggcgg
     1561 cggcgggagg aacccattca ttgacggctc ccacgcggat gacgatcggt tcgatccaga
     1621 ccaaccaaaa ttacctgagc ggggacattg cggaggtgct ggtttacggg cgggcgctgg
     1681 cggaccacga attgcagtgg gtggaattcc atctcggcaa taaatacaat cttgcggcgg
     1741 tggcgaacca ggattggaac gagtttttcg acgcgtggga aatgaagtat ttcggtcaca
     1801 cgggtgttaa tgcggcggag gacggggatg gagatgagaa gagcaatttg gatgagtatc
     1861 aggaaggcag cgatccgacg gactactata atgggcagac tccgggggtt gccgcattgg
     1921 gcggaaacaa tcaaagcggg cccttgtcgg cctttttgac ctatcccttc acggtgaaag
     1981 tttcggattc ggcggggaat cccctgaaaa acgcgccggt gaccttcacg atgagccagg
     2041 gcgccgggaa actggcgctg gcgaatacgg cggagtcggc tttggtggat acgctgagcc
     2101 tgagaaccga tggccagggg ctggcgcaag tctattaccg cggggcggac caagcggaag
     2161 tggccgccat ccaggcggtg gccggaacgg ctccgctcca ggcgcaggca ttgtttacgg
     2221 cgggcacggt gccggcggac gtgctgggcg gcggactcaa gttatggctc aaagcggatg
     2281 cggtggctca gtcgagtggg acgggagtgg cgcaatggag cgatcaatcg ggcaataacc
     2341 accatgccat tcaggccacg gcggcgaaac aacccaagta tcaactcaac gtcctcaatg
     2401 gaaagccggt ggtgcggttt gacgggatcg atgatcaatt ggcggtgacc cgcgtggtgc
     2461 aggatgactt tacgatcgcc gtgctcttca agagcagcca aggcgtggga atacagacga
     2521 agtggtgggc gggcgcgggt ctggtggacg ccgaggttgg cggtccgagg gatgatttcg
     2581 ggctgagtct caatgcgaac ggacaggtat tgggcggaac gggaggcgag aacctcgccg
     2641 acaccttcct ctattcggga acgggatata acaatgggca ggggcgcgtg gcggtatttc
     2701 gccgcgtgaa agcgaccggc gctctggagc tttacgtgga cggaattctc caagcgacgg
     2761 cggcgggtgg gacgcagccg ttggatgagg cgcaatggat gatgatcggc tccatccaaa
     2821 ccagcatgaa ttacctgcaa ggggacattg cggaggtggt ggtttacgag caagccctga
     2881 tgccggagga tcaacggcgg ctggaaattt atctgggcga caagtattct ttgcccgcgc
     2941 ttcgggacac ggatgacaac gggctccccg accagtggga agacgaccat tttgaacaac
     3001 tcggggtgaa tcccaatgcc gacgcggatg gggatggatt gacgaacctg gaggaattcc
     3061 agcagagcgg cgatccgcgg gattattacc gccaggggcg gataacgatc acgcccttat
     3121 tggcggtcgt gggaggcaac aaccagagcg gccgggcgtc ggattttctg gcttccgagt
     3181 tggaggtgtc ggtggtcaat gccgccggcg gggcgccttt gaggaatgcg ccggtgacgt
     3241 ttaaagtcac gcagggcgac gcgaagctgg cgcccagcgg ggccgcttcg ccgttggtcg
     3301 cgcagaaact cgttcgcacc ggagcggacg gccatgcccg ggtgcgggtg aagctgggaa
     3361 cggcgtttga ttcccggatc caggcgcaga cgggcacggc cgagccggtt atattttccg
     3421 ccctgggctg gaagttgctc ggccaatgga cgtttgaaaa ggacagcgcg ggggccgcct
     3481 acgatgtctc cggccagcag aaccatgcga ccaaggagga gtgggcgggg ttgtcggcat
     3541 ctggaggcgc cctttcattc agcgggacga gcggttatct gcaaaccccg gttgccaacc
     3601 aggcgcaggg ggcgctgatg gcgtggattt acccgcgcgt ttccaccgcg gcgcccgcct
     3661 ttcagagcat attcgattcg gatgtttatg gtcaattcgg gaccggatgg gggctgaaca
     3721 acgggatgat cacggtgctc ctcgacaatc tggtttggaa tacgggggtg ccggtggaat
     3781 acgacaagtg gcagcatgtg gccctcgttt atgacgcgag ttcggccaag ctctatgtga
     3841 atgggttagt gaaggccact tgcacttata cgcagggcgc tgtcacgccg gcgaattacc
     3901 ggatcgggcg aagcaatgcc tatgcccact atttcgatgg caaacttgac gacgtgcgca
     3961 tctacagcgg caccttgctg gccatggacg tcgcgctcat cagcggaggc gatgccgatg
     4021 gggacggatt cagcaatgcg caggaggtgt tgctgggcac gaattaccag gcagcggata
     4081 ccgatgggga cggattgagc gatggcgagg aggcgggctt caggattccc ctttccaccg
     4141 gcaccgaccc cacgaaggcg gatagcgatg gaaatggaat caacgacggg caggagttcc
     4201 tcaaggtgcg ggtgggcatt tcggattacg atctctatga cgatatctgg gatgtctatg
     4261 tcaacgggat atgggtggtg cgcaatgtcg cgactttcaa gttcatggaa tcgaagcccg
     4321 cctataaaac gattttcctg aagaagggaa cgacggcgaa attcaccctg gggcgtccgg
     4381 acctcaatca gctgggttcg agcgacgaat accatctgcg atttgaaaat ccagccggcg
     4441 cggagctgcc gatcgcctgg caggccgccc cggggaattc cccgttgatc agcgcgtttt
     4501 attacaatgg cgacacgcct ccggacccca gcgccctgaa atggaatttt tacatttccg
     4561 actccaacga cggggatgcc gacgggttgg atgcgaacaa ggaaaacagc cttggcacgg
     4621 accccgcgga tcccgacacg gaccatgacg gaatgcccga cggctgggag gtggatcact
     4681 cattgaatcc ccggagcccc aacgatgcgc cccgggatgc cgatggggat ggcgtgacca
     4741 atctcgagga gtatcgctac gaaggcgacc cgcacgacgg ggatgccgat ggagacggtt
     4801 tgcccgatct ctgggaaatg aaggaaggcg ccttcctggc ctccgacgat gcggccaagg
     4861 ccagttatca ccacaacggg ctgaggcatc tggagcagtt ttcgaagccg gcaaccgata
     4921 cggacgggga cggcctcagc gactacgcgg aaggcctttg gcagacgcag ccgggcgtcc
     4981 cggacacgga cggagacggc atgccggacg gttacgaggt ggccaatggc ctgaatccaa
     5041 aggacggcca ggacgccctg gcggataagg acaacgacgg ggtggcgaat ctcgcggagt
     5101 atcaagcggg gacgctgggg tgggactacg acacggataa cgacgggctt tccgatgggt
     5161 gggagcggca gtatggcact ccggccaagt cctcgatgga ggggctgcaa gcctggtggc
     5221 ggatggatca tggacccggt tacccgctgt atgacacgag cggcaatcgc cacgatgggg
     5281 agtggcatgg ggacgcgagc ctcctgcaac gcctggagcc ggagatttgg gccggcaatt
     5341 atccctaccg gagcgtgagc gcgtgggaca ggagcgcgct ggtgctgagg ggtgcgcaca
     5401 gttttgtgac cgtgccggag ggcgaggggc tggtgccgag cggaagttgc acctgggcct
     5461 tcgaggtcaa tccggacgac gtcgcgcgcg agcggacaac gacgctgttt gagaaggagg
     5521 ggagttatgc ggtgcgcctg ggttcggatc gcaagctgga ggtggagctc gtgggcaaaa
     5581 cgggagtgcg ccggaagttt ttcacggcgg gagcgctggc cgccgggcaa tggacgagcg
     5641 tgggcattgt gctggatgcg gggaatcctc ccaaggtgcg cctttatttg aacggggtaa
     5701 tgaactcgga agcgagcttg ggcgaggcgg agttggggaa gacggaccgg gcattgatcc
     5761 tcggcagcgc gcggatcgag ccgccgatgg cgccgcaggc gcagctcgac gacctccggt
     5821 tttacggccg gacgctgagc gcggcggagc tcgcggggct gag
//
LOCUS       DATDAY010000038         2569 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_2199767, whole genome shotgun sequence.
ACCESSION   DATDAY010000038 DATDAY010000000
VERSION     DATDAY010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2569)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2569)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2569
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2199767"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            7..>2569
                     /locus_tag="VIS74_00810"
     CDS             7..>2569
                     /locus_tag="VIS74_00810"
                     /inference="COORDINATES: protein motif:HMM:NF019311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA14 domain-containing protein"
                     /protein_id="HEY5791805.1"
                     /translation="MALEGGDSGSSAGAGVLLNGLGHSVVWTDFLVQPAFRDASLAFP
                     ELEAETVAGFYFDTGGCLRVYDGATGQWRRLPESPRPVNLLPVNAGAWARVTVRLNYG
                     TQRWSIWLNGVPAASDLGFAHPKTYFDRFGMASASRALLDGIRIGAETPAGLVEAIAD
                     TEPPGIPAGPRATPMSESAVRIEWAASQDNVSVKSYRVYRDGVLFAETAGLSYVDATA
                     APGASYTYSVSAVDEWGLESARSPEVAGAAAVLRRSGLGLRGTYFSRLFLTGLGVEGI
                     EGPVDFALARDVAPMPGVNHDRFSARWTGLVEPALTGTYAFQVTADDGVRLWVDGRLV
                     VDLWRYGAAETATEEITLEAGKMSTIVLEYFDYIFDAKASLKWKPPGATAFAVIPAES
                     LYARPLGVPGAAGGAPLPPEGLQAVETKDNAIRLAWKPSAAGAPAAAYRVLREGEEIA
                     RTFTPVWHDAELNPATPYHYTVRAENFDGTLSAESEPLTASTGAGMLPEPWRHRDIGE
                     PKTAGKTEWANGSFTIEGAGRSLYDAAPGGDPDQLQLAYRKMSGDFMITARVDSFEAA
                     PPQTSYEVTRPGARAGVMIRGATEGHDAFFATSVGEDGVQPAWFARAEANAAASKDLA
                     QPSFAAPCWVRVIRTENALIGAYSEDGVRWQTLLSKNVPMEEEVYAGLFVSANHPTEP
                     ARAVFSEVTVSSAVELGGLTGGGDSDGDGITDWQELNIFHTDPTVADIWIAGAVAAFF
                     GSQGTGVLGSWAVEGNALVSQTVKASVEYEVTVTEAGIHRIEAEVAAGPNATSNPIFP
                     VSLWIDGQLLEEFAVYLSDGNSALLQAATPWLQPGVHTIRLHYDNTRSYRALKVLG"
BASE COUNT          421 a          781 c          930 g          437 t
ORIGIN      
        1 aaatcattgg cgctggaagg cggcgattcg ggatcgagcg cgggggccgg cgtgcttctc
       61 aatggactcg gccactcggt ggtgtggacg gatttcctgg tgcaacccgc atttcgcgac
      121 gcttccctgg catttcccga acttgaagcc gaaaccgtgg cgggttttta ttttgacacg
      181 ggcggctgcc tccgggtgta tgacggcgcg accggccagt ggcgccggct gccggagtct
      241 ccccggcccg tcaacctgct cccggtgaat gcgggcgcct gggccagggt gaccgtgcgg
      301 ctgaactacg gaacccagcg ctggtcgatc tggctgaatg gcgttccggc cgcttccgat
      361 ctcggatttg ctcacccgaa gacctatttt gaccggttcg ggatggcgag cgccagccgg
      421 gctttattgg acggaattcg gattggcgcg gaaacgcccg cgggtttggt cgaagcgatt
      481 gcggacaccg aaccgccggg cattcccgcg gggccgcggg ccacaccgat gagcgagagc
      541 gccgtccgga tcgaatgggc ggcctcgcag gacaacgtga gcgtcaaatc ctaccgggtt
      601 tatcgcgatg gggtgctgtt cgccgagacg gcgggactct cctatgtgga tgccacggcg
      661 gcgcccgggg ccagctacac ctactcggtg agcgccgtgg acgagtgggg gcttgaatcg
      721 gcccggtctc cggaggttgc gggcgccgcg gcggtgctgc ggcgttcggg cctgggattg
      781 cggggaacct atttcagccg cctgttcctg accggtctcg gcgtcgaggg gatcgagggg
      841 ccggtggatt ttgcgctggc cagggacgtt gctcccatgc ccggggtgaa tcacgaccgc
      901 ttttcggcgc gctggacggg gctcgtcgag cccgcgctta ccggaaccta tgccttccag
      961 gtcacggcgg acgatggcgt gaggttgtgg gtggatggcc ggctggtggt cgatctctgg
     1021 cgctatggcg cggctgagac cgcaacggaa gaaatcaccc tggaggcggg aaagatgagc
     1081 acgatcgtgc tcgaatactt cgactacatc ttcgacgcaa aggccagcct gaagtggaaa
     1141 ccgccggggg caaccgcgtt tgccgtgatt ccggcggagt ccttgtatgc gcggccgctc
     1201 ggagtccctg gggcggcggg cggcgcgccg ctgccgcccg aaggactcca ggccgtcgaa
     1261 accaaggaca acgccatccg gctcgcgtgg aagccttccg ccgccggcgc ccccgcggcc
     1321 gcctaccggg ttctgcgcga gggcgaggaa atcgcgcgca cgttcacgcc ggtgtggcac
     1381 gatgcggagc tgaatccggc cacgccgtat cattacaccg tccgggctga gaatttcgac
     1441 gggaccttgt cggcggagag tgaaccgctg acggcaagca cgggcgccgg catgttgccc
     1501 gagccgtggc ggcatcggga catcggggag ccgaagacgg ccggaaagac ggaatgggcg
     1561 aatggaagct tcaccatcga gggagccgga cgcagccttt acgatgccgc gccgggcggc
     1621 gaccccgacc agctccagtt ggcctaccgc aagatgtcgg gggatttcat gatcaccgcc
     1681 cgcgtggatt cgtttgaggc ggccccgccg caaacctcct acgaggtcac ccgtccgggc
     1741 gcgcgggccg gggtgatgat tcgcggcgcc acggaggggc acgacgcctt tttcgcgacc
     1801 tccgtgggtg aggacggagt ccagccggcc tggtttgcgc gggcggaggc caacgcggcg
     1861 gcctcaaagg atttggcgca gccgtccttt gcggcgcctt gctgggtgag ggtgatccgg
     1921 accgaaaacg cccttatcgg cgcctactcg gaggacggcg tccggtggca gacgctcctc
     1981 agcaaaaacg ttccgatgga ggaggaagtt tacgcgggac tctttgtgag cgccaaccat
     2041 ccgaccgagc cggcgcgggc ggtcttcagc gaagtgacgg tgagcagcgc ggtcgaactg
     2101 ggcggcttga ccggaggagg cgacagcgat ggcgacggca tcacggactg gcaggagctc
     2161 aacattttcc acaccgatcc cacggtcgcc gacatctgga tcgcgggcgc cgtggcggcg
     2221 tttttcggtt cgcagggcac gggggtattg ggttcctggg ccgtcgaggg aaatgcgctc
     2281 gtttcgcaaa ccgtcaaggc cagcgtggaa tacgaggtga ccgtgaccga ggcgggaatc
     2341 caccggatcg aggccgaggt ggccgccggc ccgaacgcca cgagcaaccc gattttcccg
     2401 gtgagcctgt ggatcgacgg gcaactgctc gaggaatttg cggtttacct gagcgatggc
     2461 aattccgcgc tgctgcaggc ggccacgccg tggctgcagc ccggggttca cacgatccgg
     2521 cttcactacg acaatacccg aagctaccgg gcgctgaagg tgctcggcg
//
LOCUS       DATDAY010000039         2529 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_1355040, whole genome shotgun sequence.
ACCESSION   DATDAY010000039 DATDAY010000000
VERSION     DATDAY010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2529)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2529)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2529
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1355040"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(162..1598)
                     /locus_tag="VIS74_00815"
     CDS             complement(162..1598)
                     /locus_tag="VIS74_00815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5791806.1"
                     /translation="MDPVYQCLRQLRRRLWWQCWARGLAASAVGIGAAEIALGFARIV
                     LGLPPFDAINLAAGLILAPLLAALLTLRRSWNWQAMAAAADELGRSRDRFRSYLEFSA
                     EKSPSVLQNLALRETRAFAAGRQIRPPRGRWGILLGLAPVLAFGLVLQLAYFYSANRL
                     KAAREAAAKLMESAAPEVAALARRWQDPRLEKAAEALREQAAEMSRRLSRDPRRAALD
                     AIARSEEAVRNAAGNLAELAEAAQALAEAAESPELREAIGQRNYAQAAGIARQMPDEQ
                     VRRAIAEAAAARNLPALDRLREIPNPGEAAAQMLRQAERSAERNREMEKLLAALRDLK
                     LQPGPGDKSKPDQAAPASAANEAAAAVNPEPSGEGNETTASVESPAGQGEKDGGGEGA
                     ALRPADLTPPPETAARKELLASLLGEGGSLQDLVLSGAGDSEAKRRYQAIYQAAQADE
                     AAAVERENIPVRSRSVVKRYLESIRPGN"
     gene            1634..2350
                     /locus_tag="VIS74_00820"
     CDS             1634..2350
                     /locus_tag="VIS74_00820"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HEY5791807.1"
                     /translation="MRVHVFQHVPHEGPAAIGAWASARGHSVATTHLFAGEPLPSLED
                     FEMLVVMGGPMNIYQYRDHPWLKPERRFLEKAISAGKKLAGACLGSQLLADALGARVT
                     QNAQVEIGWHPIRFSAEARRRFPALPESQLALHWHGDTFELPEGAIRLAESDACAEQG
                     YWYDDRVLGLQFHPEITPEVLKIWCEVDAPQPGGFVQSPAAILNQAEERYAESHRTLF
                     TLLDALDLISQKRFPKSDSL"
BASE COUNT          415 a          862 c          808 g          444 t
ORIGIN      
        1 agcagatcct ccagcacctc gccctggccg acgatgcgct tccgcatctc ggtcttgagt
       61 cgatggaaaa gccggcggaa ttcctcggct tcctgcgcgg cgagttcggg agtcattgac
      121 gggatttaac catagggaac accggggaca cagagaaaat gtcagttccc gggccggatg
      181 ctttccagat aacgcttcac cacgctgcgc gagcgcacgg ggatattctc gcgttccacc
      241 gcggcggcct cgtccgcctg cgcggcctga taaatggcct gataacgccg cttcgcctcc
      301 gaatcgccgg cgccggaaag caccagatcc tgcagcgagc cgccttcgcc gagcaggctg
      361 gcgagcaatt ccttccgcgc cgcggtctcc ggcggcgggg ttaaatcggc gggccggaga
      421 gcggctccct cgccgccgcc gtccttttcg ccctggcccg ccggcgattc gaccgaggcg
      481 gtggtctcgt ttccttcgcc cgaaggctcc ggattcaccg ccgccgcggc ctcattcgcg
      541 gcggacgccg gcgccgcctg atccggcttc gatttgtccc ccggacccgg ctggagtttg
      601 agatcccgca gcgccgcgag cagtttttcc atctcccggt tgcgttccgc ggaccgctcg
      661 gcctgccgca gcatctgcgc ggcggcttcg ccggggttgg gaatctcgcg caggcggtcg
      721 agcgcgggca gattccgcgc cgcggcggct tcggcaatgg cgcgccgcac ttgttcgtcc
      781 ggcatttgcc gggcgatccc ggcggcttgc gcgtaattcc tttgcccgat cgcttcgcgc
      841 agttccggcg actccgccgc ctccgccagg gcctgggcgg cctccgcgag ctcggccaaa
      901 tttcccgcgg cgttccggac ggcctcctcg gaccgggcga tggcgtccag cgcggcgcgg
      961 cgagggtcgc ggctcagccg ccggctcatt tccgcggcct gctcgcgcaa ggcttcggcg
     1021 gccttttcca accgcggatc ctgccagcgg cgcgccagcg cggcgacttc cggcgcggcg
     1081 gattccatca gtttcgccgc ggcttcccgc gcggccttca ggcggttcgc ggaataaaaa
     1141 tacgccagct ggaggaccag cccgaacgcc aaaaccggcg cgagcccgag gaggatgccc
     1201 catcgcccgc ggggcggacg gatttgccgc cccgcggcga acgcccgggt ttcccgcagc
     1261 gcgagatttt gcagcacgct cggggatttt tccgcggaaa attcgagata ggagcggaag
     1321 cgatcccgcg agcggccgag ttcgtccgcc gcggcggcca tggcctgcca gttccacgaa
     1381 cgccgcaggg tgagcaaggc ggcgaggagg ggagcaagga tcagcccggc agcaagattg
     1441 atggcatcaa acggcggcag ccccagcacg atgcgcgcga atcccagcgc gatttcagcc
     1501 gcgccgatgc ccaccgcgct tgccgcgagc ccgcgcgccc agcattgcca ccaaagccgc
     1561 cgccgcagct ggcgaaggca ttgataaacc ggatccattc tgcttcatat aacaccgcac
     1621 aaggcttccc acaatgcgag tccacgtttt ccagcatgtc ccgcacgaag gcccggcggc
     1681 catcggcgcc tgggcgtccg cccgcggcca ctccgtcgcc acgacccacc ttttcgccgg
     1741 cgagccgctg ccgtcgctgg aggatttcga gatgctcgtg gtcatgggcg ggccgatgaa
     1801 tatctaccaa taccgcgatc acccctggct caagccggag cggcggtttt tggaaaaagc
     1861 gatctccgcc ggcaaaaaat tggccggcgc gtgcctgggc tcacaactcc tcgccgacgc
     1921 gctgggcgcg cgcgtcacgc agaacgcgca agtagaaatc ggctggcacc ccattcgttt
     1981 ttccgccgag gcgcggcggc ggtttccggc tttgcccgaa tcccagctcg ccctgcactg
     2041 gcacggcgac acgtttgagc tgcccgaggg cgcgatccgc ctggcggaaa gcgacgcctg
     2101 cgccgagcag ggctactggt atgacgaccg cgtgctgggg ctgcagttcc atccggaaat
     2161 cacgccggag gttttgaaaa tctggtgcga agtggacgcc ccgcagccgg gcggatttgt
     2221 gcaaagcccg gcggccattc tcaatcaagc ggaggaacgc tacgccgagt cgcatcgcac
     2281 cctcttcacg ctgctcgatg cgctcgatct catttctcaa aagagatttc cgaaaagcga
     2341 ttcattgtag aggcggttgt gtcaaccgcc tgtcgttacc aaaggcaacg cgctgggtat
     2401 ttcggaagct tcttttaacc cgaaaacgga ttttgaatag aacacagaga gaagatgccg
     2461 ggggaaaagc cccggggagg ttttcattct gcgttttact ttcggtggtc cctgtatcct
     2521 cggtggttt
//
LOCUS       DATDAY010000040         2244 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_1843440, whole genome shotgun sequence.
ACCESSION   DATDAY010000040 DATDAY010000000
VERSION     DATDAY010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2244)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2244)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2244
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1843440"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(29..532)
                     /locus_tag="VIS74_00825"
     CDS             complement(29..532)
                     /locus_tag="VIS74_00825"
                     /inference="COORDINATES: protein motif:HMM:NF013231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HEY5791808.1"
                     /translation="MAKKTPTSETPETSLIKQATQRHAGSHLDASLLWDQVFEVGAAL
                     IDHESKVLSKYKLSRSRLRVLLLLNEEPKRRPSYLAETLGITKASITGLAESLSKDGL
                     ISAAANTRDRRSHTLSITAKGKGLLKKTLPGYYPKLAEALASLSRQEQAQLSQILQKW
                     VSGIRKA"
     gene            656..829
                     /locus_tag="VIS74_00830"
     CDS             656..829
                     /locus_tag="VIS74_00830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5791809.1"
                     /translation="MKFPLAGFRLRFASLIGMIPDGSAAAFSPNGGVNAATCAVENFP
                     ATGPGGFNLLGLS"
     gene            complement(844..1944)
                     /locus_tag="VIS74_00835"
     CDS             complement(844..1944)
                     /locus_tag="VIS74_00835"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF018613.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY5791810.1"
                     /translation="MKRVLILTASFGEGHNTAAKSLRQAVLAVAGADTEVLVVDPYSK
                     TNPKFNRLIQVGYSVMINRYPKIWNFTFSILSLPGLLEGMLPAMQALQHAIRDLWRDF
                     QPDIVVSTYPVFSFFVARLRKTHAEFREPFVMVVTDSTRINSAWYRCWSDQFVVPDQS
                     TVRVLVKAGVPPERVQALGFPVSLEFEKTRPISPAEGAPWRVLYMPSTKKGWALQAVR
                     EILRIPQVDLTILTAKNAALREQLDHAGISQKDNVHVLGWTDQMPHLMAASHVFVGKA
                     GGAVVQEALAAECPFVISHVVPGQEEGNMDLILDGQMGAMAAGSPKEIRQTLEQAFAE
                     DGKLWRHWKRNIARHSTKHAARRIAEFLAGQI"
     gene            complement(1949..>2244)
                     /locus_tag="VIS74_00840"
     CDS             complement(1949..>2244)
                     /locus_tag="VIS74_00840"
                     /inference="COORDINATES: protein motif:HMM:NF012684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
                     /protein_id="HEY5791811.1"
                     /translation="QNGATALKMLKAKLYQIEADKKRAEAEREYGEKGDIAWGNQIRS
                     YVFQPYQMIKDLRTGVQTSDIQSVMDGGIDAFIEGKLRGLKYVKGAASGEDED"
BASE COUNT          383 a          672 c          686 g          503 t
ORIGIN      
        1 tcatgacgcg ggaaagcggg gtaggctgct aggctttccg gatgccgctc acccattttt
       61 gcagaatctg gctcaattgc gcctgttcct gccgggacag cgacgccagc gcctcggcga
      121 gtttgggata atagcccggc agcgtttttt tcagcaggcc cttgcccttg gcggtgatcg
      181 aaagggtgtg ggaacggcgg tcccgggtgt ttgcggcggc gctgatcagg ccgtccttgc
      241 tcagtgattc ggccaagccc gtgatgctgg ccttggtgat gcccaaggtt tccgccaggt
      301 aactcgggcg ccttttcggc tcctcattga ggagcagcag cacgcgcagc cggctgcggc
      361 tgagcttata tttgctgagc accttgcttt catgatcgat caacgcggct cccacctcga
      421 agacctgatc ccagagcagg gaggcatcca ggtgagaacc cgcgtggcgc tgggtggcct
      481 gtttgattaa cgaggtttcg ggggtttcgc tggtaggtgt cttcttcgcc atgattatca
      541 atttatttga atataaatta cattaacaat tagcttcctt aagtcgtcaa gggaagattt
      601 tcaatttggc ggcggaattc ggtttttttt gaagcaaatc gattaaatcc ggggcatgaa
      661 gttccctctc gccggtttcc ggcttcggtt tgcttcgctg attggaatga tcccggacgg
      721 ttccgccgcc gctttttcgc caaatggcgg ggtgaatgcc gccacctgcg cggtggaaaa
      781 tttccccgcc accggcccgg gcggcttcaa tctccttggc ctgtcctgag cgggcgttcg
      841 gagtcaaatc tggccggcca gaaattccgc gatccgccgc gcggcgtgtt tggtgctgtg
      901 ccgggcgatg tttcgcttcc agtgccgcca gagtttgccg tcctccgcga aggcctgctc
      961 cagggtctgc cggatttcct tcggcgagcc ggccgccatg gcgcccattt ggccgtccag
     1021 aatcaggtcc atgttgcctt cctcctggcc gggcacgacg tggctgatga caaacgggca
     1081 ttcggcggcg agggcttcct gcaccacggc tccgcccgcc ttgccgacaa acacgtggct
     1141 ggcggccatg aggtgcggca tctggtcggt ccagccgagc acgtggacat tgtccttctg
     1201 gctgataccg gcgtggtcga gctgttcgcg caacgcggca tttttggccg tgaggatcgt
     1261 gagatcgacc tgcgggattc gcaaaatctc ccgcaccgcc tgcagggccc agcctttttt
     1321 ggtcgaaggc atgtagagca cccgccaggg cgcgccttcg gccggggaaa tgggccgcgt
     1381 tttctcgaat tccagcgaaa ccggaaaacc gagcgcctgg acgcgctccg gcgggacgcc
     1441 ggccttgacc aatacccgca cggtggattg gtccgggacc acgaactgat ccgaccagca
     1501 ccgataccag gcggagttga tccgcgtgga atccgtcacg accatgacga agggttcccg
     1561 gaattccgca tgcgtcttgc gcagccgcgc gacaaaaaag ctgaagaccg gatacgtcga
     1621 aacaacgatg tccggctgaa agtcgcgcca cagatcgcga atggcgtgct gaagcgcctg
     1681 catggccggc agcatccctt ccagcagccc gggcaggctc aggatgctga acgtgaaatt
     1741 ccagatcttc gggtatcggt tgatcatcac cgagtaaccg acctggatca ggcgattgaa
     1801 cttcggattg gtcttggagt agggatccac caccaatacc tccgtgtccg ccccggcgac
     1861 ggcgagcacg gcctgccgca ggctcttggc cgcggtgttg tggccctcgc cgaaacttgc
     1921 ggtcaggatc aggacacgct tcatggagtt aatcctcgtc ttcgccggag gcggctccct
     1981 tcacgtattt caggccgcgc agcttgcctt cgatgaaggc gtcgatgccg ccatccatca
     2041 cgctctggat gtcgctcgtc tgcacgccgg tgcgcagatc cttgatcatt tgatacggct
     2101 gaaacacgta ggagcggatt tggttgcccc acgcgatgtc gcccttctcg ccgtattcgc
     2161 gctcggcttc cgcgcgcttt ttgtcagctt cgatctggta gagcttcgcc ttcagcatct
     2221 tcagggccgt cgcgccgttt tgcg
//
LOCUS       DATDAY010000041         3305 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_606493, whole genome shotgun sequence.
ACCESSION   DATDAY010000041 DATDAY010000000
VERSION     DATDAY010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 3305)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3305)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3305
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_606493"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..759)
                     /locus_tag="VIS74_00845"
     CDS             complement(<1..759)
                     /locus_tag="VIS74_00845"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEY5791812.1"
                     /translation="MFVQSVGLAQEDVAQKALARSLNDAFADVYEKVSPSVVVIEAKM
                     LPNQADGARDAWEFFFQGPGFQTPGQLPGASVPPKEGSGLIIRTDGYIVTNQHVLRGA
                     VADGITVTLKDGRKLPAKLAGADERTDVAVLKVDAKDLAAAELGDSDKVRVGQFAFAL
                     GAPFDLPYTFTFGLVSATGRNDLLHLPDAYEDYIQTDAAINPGNSGGPLADIDGRVIG
                     MNTLINGLNRGLGFAIPVNLVKDVSDQLIAKGRVA"
     gene            899..2386
                     /locus_tag="VIS74_00850"
     CDS             899..2386
                     /locus_tag="VIS74_00850"
                     /inference="COORDINATES: protein motif:HMM:NF027583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4912 domain-containing protein"
                     /protein_id="HEY5791813.1"
                     /translation="MKPSAQKRPAKHQEKGAFEFAPKLDTPSSNAARSAASKKSVAAP
                     PPKTTGHAPALPRTYGFETLFLIAQDPHWLFTYWDIDISRHPGGQTFLRCYHGNKEIE
                     KEIEVPFETRNWYIPVTVAGARYHVELGYYRGETWNAIAVSDTVDTPPENLSESDRFD
                     FATIPLHLSFSKLMENIQQGIQSGETLVSALSRLQRDGKLLPLQPSDFASLGSDERKV
                     LETLLGREFLESYSGSSLSSHELQELIRKRLEERLNSEAGSEWLASWSGAGAESSLFG
                     LLQHLAGPTSWSTAALSSWAAAALMSWAAAARGLSSWSGGPAAGAVSSSWGPQSLMSW
                     LQAASSSWAQSTSSSLSQAALASWSQAVLTSWSQAALSSWSQAALSSWSSGETSSWSG
                     QPFGKGEFFLNVSAELIFYGSTHPNASVSIDGKPVTLAADGTFRHHFIFPTGNDYEVP
                     IVATSPNGSEVQRATLRFERETGKFGIVTDASLPPFSAAPLVRVA"
     gene            2440..>3305
                     /locus_tag="VIS74_00855"
     CDS             2440..>3305
                     /locus_tag="VIS74_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HEY5791814.1"
                     /translation="MGNRAINRSLPRHPDPWVEEFLTYLRGDRNASTHTQRNYQRALD
                     AFRAFRPEGNWNRDQPEDFRNYLFALMKQKRRATVRLEFSALRAFYKFLIFRNKIKTN
                     VLAEVILPKSEKKLPHFLTSGQIEKLLTSPLRAPVEKQAPAWMPLRDAAILELFYGAG
                     LRLSELAALDVRDVDSISETVRVMGKGSKERICPIGEPAATAIQKYRQAAQVQHGPLF
                     ISKLRGRLTSRSIWLLVKKYLELEGLPITISPHKLRHTFATHLLDGGADLRSVQTLLG
                     HSSLSTTQIYTHV"
BASE COUNT          696 a         1073 c          868 g          668 t
ORIGIN      
        1 cgccacgcgg cctttggcaa taagttgatc gctcacgtcc ttcaccagat tcaccggaat
       61 ggcgaatccc agtccgcgat tgagaccgtt tatcagcgta ttcatgccga tcacccggcc
      121 atcaatgtcc gccagcggcc cgccgctgtt gccgggattg attgcggcat ccgtctgaat
      181 ataatcctca tacgcatccg gcaaatggag aagatcgttg cgtccggtcg cgctcacgag
      241 tccaaaggta aacgtgtagg gcagatcaaa cggcgccccc agggcgaagg caaattgccc
      301 caccctcacc ttgtcgctgt cgcccaattc cgcggcggcg agatccttgg catccacttt
      361 caaaacagcc acatccgtgc gctcatcggc gcccgccagc ttggcgggca gcttccgtcc
      421 atccttcagc gtcacggtaa tcccatccgc caccgctccg cgcagcacat gctgattggt
      481 gacgatgtag ccatccgtcc gaatgatcaa tcccgagccc tctttcggcg ggacactcgc
      541 gccgggcaac tgcccgggcg tctggaaacc cggcccttga aaaaagaatt cccacgcgtc
      601 gcgcgcgccg tccgcctggt tgggaagcat ttttgcttca atgacgacga ctgatggcga
      661 aactttttca tacacgtcgg cgaaggcgtc attgaggctc cgcgcgaggg ctttttgagc
      721 aacgtcctcc tgcgcaagtc caacggattg aacaaacaat aaggtaatta accaagtaaa
      781 ccaatggtgc acaaaatgaa agttactcac gacgaaatca ttttcaagtg aacctctcca
      841 tgaaaaaagt ctttgcaagc aggcagaatc atgggactaa ggatttcagc gggaagtaat
      901 gaagccatca gcacaaaaac gaccagccaa acaccaggaa aagggagcgt tcgaatttgc
      961 tcccaaactg gatactccgt catccaacgc cgctcgttca gccgcgtcta aaaagtcggt
     1021 cgccgctcct cctcccaaaa caactggtca tgccccggcc ctgccccgca cctacgggtt
     1081 tgaaaccctt tttctcatcg cccaggatcc ccattggctt ttcacctact gggatatcga
     1141 tatttcccgg cacccgggcg ggcagacttt tttgcggtgt tatcacggaa acaaggaaat
     1201 cgaaaaagag atcgaagttc ctttcgaaac ccggaattgg tatatccccg tcaccgttgc
     1261 cggcgcgcgg tatcacgtcg agcttggcta ctaccgcgga gaaacctgga acgcgattgc
     1321 cgtctcggac accgtggaca ctccgccgga aaatctttcc gaatccgacc gcttcgactt
     1381 cgccaccatt cccctccacc tgagtttctc gaaactcatg gagaacatcc agcagggcat
     1441 ccagtccggc gagaccctgg tttccgcgct ctcccgcctg cagcgcgacg gcaagctgct
     1501 tccgctccaa ccctcggatt tcgccagcct cggttccgac gaacgcaagg tgctcgaaac
     1561 cctgctcggc cgggaattcc tggaatctta cagcggttcc tcgttaagct cccacgaact
     1621 ccaggagctc attcgcaagc ggctcgaaga acggttgaat tccgaagctg gcagtgaatg
     1681 gctggcttcc tggagcggcg cgggagccga atccagcctt ttcggcctcc tgcaacactt
     1741 ggccggcccc accagttggt ccaccgccgc gctttccagc tgggccgccg ccgccctcat
     1801 gagctgggcc gccgccgccc gcggtctttc aagctggagc ggaggaccgg ccgcgggagc
     1861 agtttcatcc tcttggggac cgcaaagcct gatgtcctgg cttcaggctg cctcctccag
     1921 ctgggcgcag tccacctctt ccagtttgtc gcaggcggcg cttgccagtt ggtcacaagc
     1981 cgtcctcacg agctggtcgc aggcggcact ttccagctgg tcacaagccg cgctttccag
     2041 ttggagttcc ggcgaaacct ccagctggag cgggcagccc tttggcaagg gcgaattttt
     2101 cctgaacgtc agcgcggagc tgatctttta cggcagcacc catccgaatg cctccgtgag
     2161 cattgatggc aaacccgtta ccctcgcggc cgatggcacc ttccgccacc acttcatctt
     2221 tcccacgggg aatgattacg aggtgccaat cgtggccacc tcgcccaatg gcagcgaagt
     2281 ccagcgggcc acgctgcgct ttgagcggga aaccggcaaa tttgggatcg ttaccgacgc
     2341 ttccctgccg ccgttcagcg ccgccccctt ggtgcgggtc gcctaaccgt gcgctccgcc
     2401 agctcatccg ggcaatctga aaaaaagcgt cccgcccggt tgggaaatcg agccatcaat
     2461 cgatctctac cccggcatcc cgatccgtgg gtggaggaat tcctgaccta tcttcgcggg
     2521 gatcgcaacg cttccactca cacccagcgc aactaccaac gcgccctgga tgccttccgg
     2581 gcttttcgtc cggaaggaaa ttggaaccgc gaccagccgg aggatttccg gaactatctg
     2641 ttcgcgctca tgaaacaaaa acgccgggcc acggtgcggc tggaattcag cgcgcttcgg
     2701 gcgttttaca aatttctcat ttttcgcaac aagattaaaa ccaatgtatt ggcagaggtt
     2761 attctgccaa aatcggaaaa aaagctcccc cattttctga cttccggcca gatcgagaag
     2821 ctcctcacct ccccgctccg cgctcccgtg gaaaaacagg cgcccgcctg gatgccgctg
     2881 cgggatgcgg ccattctcga gttgttttac ggagcgggcc tgcgcctttc cgaattggcg
     2941 gcattggacg tgcgggacgt ggattcgatc tcggaaaccg tgcgcgtaat gggcaaggga
     3001 tccaaggaaa ggatctgccc gatcggcgaa ccggcggcga cggccattca aaaataccgg
     3061 caggccgccc aggtgcaaca tggcccgctt ttcattagca agctgcgcgg ccgcctgacg
     3121 tcccggtcga tctggctgct ggtcaagaaa tacctggaac tggaaggttt gcccatcacc
     3181 atcagccccc acaagttgcg gcacaccttt gccacccacc ttctggacgg cggcgcggat
     3241 ttaagaagcg tgcaaaccct gctgggccat tccagccttt ccaccaccca gatttatacc
     3301 cacgt
//
LOCUS       DATDAY010000042         2028 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_1841446, whole genome shotgun sequence.
ACCESSION   DATDAY010000042 DATDAY010000000
VERSION     DATDAY010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2028)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2028
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1841446"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2028
                     /locus_tag="VIS74_00860"
     CDS             <1..>2028
                     /locus_tag="VIS74_00860"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY5791815.1"
                     /translation="SGVEWKSEENSRANGGGRTPILRVGAAMQADQGRQVIGFVVIDY
                     LAESLFQKTARTDDHLQTTTFIATADGKWVSGPGGDSVVGMETVKSVFPAAWPKIIGT
                     SSGTSFSSEGLVVFTTVPLAAESAPQAVSGAQQAWKIFTAESPLLIADRERAATAPVL
                     GWGILALCLFIPLTCLVVTDREQKREASRAREQARALLQSITDSSVDGIVAGEAGRDS
                     HGDIKDFHTVFANPAAVHILKTFDRREAAEGKEFPLFFSPDFFAHCVQVVATGVRYET
                     EQSSECPAFGTRWFRIAVIRLEDGVVMTFTDITEQKLAVHELRLAKDAAEVANRTKSQ
                     FLTMMGHEIRTPMNGLLGFASLLERTDLSDEQQDYVSTLRLSGEALLRILEDILDYSH
                     MENEALNMKIVPVAVKELITQVSQLFAMAIGDRKLELVARIASDVPPQILGDDVRLRQ
                     ILVNLIGNAVKFTAEGFILIKVLREAGPQGDFLVFHVVDSGTGIAPEMIDRLFKPFSQ
                     VDAGSNRRYGGTGLGLSICRRLVETMGGEIGVKTAPEKGSDFYFSIPIKVPDFLAVTP
                     APVAKRVRADFKKPRILVVDDDSINRKLITRMIEKVGAEAELVESGSQAIHAFQEEKF
                     DLIIMDIQMPGMDGIETTRKIREIEKRTGALRRTPISALTANAGEGDRERCF"
BASE COUNT          411 a          566 c          632 g          419 t
ORIGIN      
        1 tccggcgtgg aatggaagtc cgaggaaaac agtcgcgcta atggcggagg ccgcacgccc
       61 atccttcgcg tgggagcggc gatgcaggcg gatcaagggc ggcaggtgat cggttttgtg
      121 gtgatcgatt atctcgccga aagcttgttc caaaaaacag cccgcaccga tgatcacctt
      181 cagaccacca cattcattgc cacggctgat ggcaaatggg tgagcgggcc gggcggcgat
      241 tcggtcgtgg gcatggagac ggtcaagtct gtcttcccgg cagcttggcc gaagatcatt
      301 gggacttctt ccggcacgtc tttttcctcc gagggattgg tggtcttcac gactgttccc
      361 ctggcagccg agagtgcgcc gcaagccgtt tccggggcgc aacaggcatg gaaaattttt
      421 accgcggaat cgccgttgct catcgcggat cgggagcgag ccgcgaccgc gcccgtgctg
      481 ggatggggaa tcctggcgct atgcctgttc attcccctga cctgtctcgt tgtcacggat
      541 cgtgaacaaa agcgcgaggc ttcgcgtgcg cgggaacaag cccgggctct tctgcaaagc
      601 atcaccgact cttctgtcga tggaattgtc gcgggagaag ccgggcgtga ttcccatggg
      661 gatatcaagg atttccacac cgtgttcgcg aatcccgcgg cagtgcatat cttgaaaacc
      721 ttcgaccgcc gggaggccgc cgagggaaaa gaatttccgc tgtttttctc gccggacttc
      781 tttgcgcatt gcgtccaggt agtggccacc ggggtgcgct atgagaccga acagagttcg
      841 gaatgtccgg ctttcgggac gcgttggttc cgcatcgcgg tcatccgttt ggaggatggc
      901 gtcgtcatga cgtttaccga tatcaccgag caaaagcttg ccgtgcatga actgcgcctg
      961 gcgaaggatg ccgccgaggt ggccaatcgc accaagagtc agttcctcac catgatgggg
     1021 cacgaaatcc gcacgccgat gaatgggttg ctgggttttg cctcgttgct ggaacgcacc
     1081 gatctgagcg acgagcagca ggattacgtt tccacccttc gtctcagcgg ggaggccctt
     1141 ctgcgcattc tcgaagacat cctcgattac tcccacatgg agaatgaggc gttgaacatg
     1201 aaaatcgtgc ccgtggcggt gaaagaattg atcacccagg tgagccagct tttcgcgatg
     1261 gccatcgggg atcgcaagct cgaactcgtc gcccggatcg cctccgatgt gcctcctcaa
     1321 atcctcggag acgacgtgcg actgcgccag attttggtga atttgatcgg caacgcggtc
     1381 aaattcaccg cggagggctt tatcctcatc aaagtcctgc gcgaggccgg gccgcagggg
     1441 gattttctcg tctttcacgt ggtggacagc gggaccggga tcgccccgga aatgattgac
     1501 cgcctgttca agcccttcag ccaggtggat gccggaagca accggcgcta tggcggtacc
     1561 gggttggggc tgtccatttg ccgccggctt gtcgaaacca tgggcgggga aattggcgta
     1621 aaaaccgcgc cggaaaaggg gtcggatttc tatttttcca tcccgatcaa agttccggat
     1681 ttcctggcgg tgactcccgc tccagtcgca aaacgggtgc gggcggattt caaaaagcct
     1741 cgcatcctgg tcgtggatga cgactcgatc aatcgcaagt tgatcacgag gatgatcgaa
     1801 aaagtcgggg ccgaggcgga gttggtggaa tcgggaagcc aggccatcca cgctttccag
     1861 gaggaaaagt ttgatctcat catcatggat attcagatgc cgggcatgga cggcatcgag
     1921 acaacccgca agatccgcga aatcgaaaaa cgcaccggag ccctgcggcg cacgccgatt
     1981 tccgcgctca ccgccaatgc cggggagggc gaccgcgagc ggtgcttc
//
LOCUS       DATDAY010000043         4406 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_1705062, whole genome shotgun sequence.
ACCESSION   DATDAY010000043 DATDAY010000000
VERSION     DATDAY010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 4406)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4406)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4406
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1705062"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..115)
                     /locus_tag="VIS74_00865"
     CDS             complement(<1..115)
                     /locus_tag="VIS74_00865"
                     /inference="COORDINATES: protein motif:HMM:NF014857.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase N-terminal
                     domain-containing protein"
                     /protein_id="HEY5791816.1"
                     /translation="MKLLVIGSGGREHALAWKLAQSPRVGKIFCAPGNAGTG"
     gene            complement(120..851)
                     /locus_tag="VIS74_00870"
     CDS             complement(120..851)
                     /locus_tag="VIS74_00870"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY5791817.1"
                     /translation="MRLLFVCLSTMLLVAGCASKRNNEAPVSTGTRVEWFGHLCLRVT
                     TPIGTQILIDPFRPGSTPYSLPSSMNPDVILISRETPDCTNTAISDGTPSIYRGSVAI
                     GPNNAAGVRIVGTPIFPAVDGNAMNSLGMNLVYSWNSGGLRFCHPGVLNRGLAPSEVQ
                     QIGRVDVLFLPIDAPYGQSHAELNEIVAQLRPRVVVPIAFTSGSVNAWASSYTDVRHL
                     PGRSFVIRPDMLPNPPNPTVLIFGD"
     gene            complement(888..1697)
                     /gene="thiD"
                     /locus_tag="VIS74_00875"
     CDS             complement(888..1697)
                     /gene="thiD"
                     /locus_tag="VIS74_00875"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEY5791818.1"
                     /translation="MAKPVVLSIAGSDNSAGAGAQADLKAITAQGGYALTALTCVVAE
                     VPGKVSAVQAVKPEIVREQIRLSFEAFPIAAVKTGMLYSQAIIRTVAEELSVQFRALK
                     TKPWLIVDPVMVASSGDPLLRPGAMREYLRRLFPLADVITPNLDELSILAGEKITTLE
                     RMREAGKRLSDKYGAAFLLKGGHLRGSRAVDLLVESGRVHRFEAPFIRGVDTHGTGCT
                     YASALAAQLALGKPLPEAVAAAKHYLTSAIRRHLRWKGTHALEHFPARALA"
     gene            complement(1709..2392)
                     /locus_tag="VIS74_00880"
     CDS             complement(1709..2392)
                     /locus_tag="VIS74_00880"
                     /inference="COORDINATES: protein motif:HMM:NF016206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HEY5791819.1"
                     /translation="MTLFILLIGVPFLLGLLAQWKVSSAFKHWRNVAATSGVTGAQAA
                     QRILATANIHDVEVVEIQSMLGDHYDPSQKRLCLSPDVYRTPSVAALGIAAHECGHAV
                     QHARGYAPLAWRMAVVPATAVVSRILPFVLIAGFFIPALGYRAMMVAAVCYAVLAVFQ
                     LITLPVEFDATRRAKVILGQMRLIQPGREAAGVNSVLDSAALTYVAAFVAVLGQLLYY
                     LLILMGNRR"
     gene            complement(2471..3583)
                     /locus_tag="VIS74_00885"
     CDS             complement(2471..3583)
                     /locus_tag="VIS74_00885"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HEY5791820.1"
                     /translation="MARSSARLIYGASEQNADLLYATRFFAPDAFAFLQTSRGRFLLL
                     SDLEVDRGRRDAAVDRVDSLSEFEARAGQPGYAAALAGWLKAQRVSAARVPGDFPLGL
                     ALELQRRGIRLEAVEGSFWPEREFKTAAEAAWITRALRITEAGMSRGIEVLKAAEIRP
                     RDRRLVWGGSVLTSERLRAEIDGAILRLGGNAQNTIVAGGDQACDPHERGSGPLRAGE
                     LIILDVFPRHTASGYHGDLTRTVVKGPVSEARRKLWQTCLEGQKRALREMKPGAAGKT
                     IHEGLKAFFAERGYPTEIREGRWQGFFHGTGHGLGLEIHEAPRFGATTFKPGQVITVE
                     PGIYIPGLGGVRHEDVALVTGAGNRVLSRMPKPLEL"
     gene            3785..>4406
                     /locus_tag="VIS74_00890"
     CDS             3785..>4406
                     /locus_tag="VIS74_00890"
                     /inference="COORDINATES: protein motif:HMM:NF012762.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose binding domain-containing protein"
                     /protein_id="HEY5791821.1"
                     /translation="MTALLIFTLNAHSAEFASTAKLIATSDWGDGFVASLEIKNTSSQ
                     PVKNWTASFDLPRQIESLWNGTILSHAGNRYTVQNAGWNSEIPPGGSVLIGFQGYPGN
                     PPQPTRVKLMGDGASPSPTPTPAPTPTPSPSATPKPSATPKPSATPLPAPSATPSATP
                     SPSPSATPTPAAGAPGYPSLSVLRDWQNGGYEVTWTKWSGPNATRWR"
BASE COUNT          830 a         1510 c         1348 g          718 t
ORIGIN      
        1 cgccggtgcc cgcattgccg ggggcgcaga aaatttttcc gacccgcggg gattgcgcga
       61 gcttccaagc cagggcgtgt tcgcggccgc cggaaccgat cacgagaagc ttcattcggc
      121 tagtctccaa aaatcagcac cgtgggattc ggcggattgg gcagcatgtc ggggcggatc
      181 acgaacgagc ggcccggcag gtggcgcacg tcggtataac ttgaagccca cgcattgacc
      241 gagccggacg tgaaggcgat cggcaccacc acgcgcgggc gcagctgggc gacgatttca
      301 ttcagctccg catggctctg gccgtagggt gcgtcgatcg gcaggaacaa cacgtccacg
      361 cggccgatct gctgcacctc ggatggcgcc agcccgcgat tcagcacgcc gggatggcag
      421 aagcgcagtc cgccgctgtt ccaggaataa accagattca tgccgagcga gttcatcgca
      481 tttccatcga cggcggggaa aatgggcgtg cccacgatgc gaacgccggc cgcattattc
      541 gggccgatcg ccacgctgcc gcggtaaatg ctcggcgtcc cgtcggagat cgccgtattc
      601 gtgcagtccg gcgtctcgcg cgagatgaga atcacgtccg gattcatgct cgaaggcagc
      661 gaatacgggg tgctgccagg gcggaaggga tcgatcagga tctgggtgcc gatcggcgtg
      721 gtcacccgca ggcacagatg cccgaaccac tccacccggg ttcccgtcga aaccggggct
      781 tcattgttcc gcttcgaggc gcaaccggcg accagcagca tcgtggacag gcagacgaat
      841 aacaaacgca tgagaggggg cagtgtaaga ggcggtgaaa ccaatggtca agccagcgcg
      901 cgcgccggga aatgttcgag cgcgtgcgtc cccttccagc gcagatgccg gcggatggcg
      961 gaggtcaggt aatgcttcgc cgcggcgacc gcctcgggca ggggcttgcc gagcgccaat
     1021 tgggcggcca gggcggaggc atacgtgcag ccggtgccgt gcgtgtccac gccgcgaatg
     1081 aacggcgcct cgaaccggtg aacccggccg gattccacga gcagatccac cgcccggctt
     1141 ccgcgcagat ggccgccctt gagcaggaag gcggcgccat atttgtccga caggcgcttg
     1201 cccgcttccc gcatccgctc gagcgtggtg attttttcgc ccgcgaggat cgagagctcg
     1261 tcgaggttcg gcgtgatcac atccgccagg ggaaagagcc ggcggagata ctcccgcatc
     1321 gccccggggc gcagcagggg atcgccgctg ctcgcgacca tgacggggtc cacgatgagc
     1381 cacggcttgg ttttcagcgc gcggaactga acgctcaatt cctccgcgac cgtccggatg
     1441 atcgcctggg aatagagcat gccggttttg acggcggcga tcgggaacgc ctcgaagctg
     1501 aggcggattt gctcgcggac gatttccggc ttcaccgcct gcaccgcgga gaccttgccc
     1561 ggcacctcgg cgacgacgca ggtcagggcc gtcagcgcgt agccgccctg cgcggtgatc
     1621 gccttcaggt cggcctgcgc cccggctccg gcggagttgt cggagcccgc gatgctgaga
     1681 acgaccggct tcgccatccc cggcgccctc aacggcgatt acccatcagg atgagcaggt
     1741 aataaagcaa ttgcccgagc accgcgacaa aggcggccac gtaggtcagc gcggcggagt
     1801 ccagcacgct gttcaccccg gcggcttccc ggccgggctg gatcagccgc atctggccca
     1861 gaatgacctt cgcccggcgc gtggcgtcaa actccaccgg aagcgtgatg agttgaaaca
     1921 ccgcgagaac cgcgtagcag accgccgcaa ccatcatggc ccgatacccc agcgcgggaa
     1981 tgaaaaaccc cgcgatcagg acaaagggca aaatccgcga gaccaccgcc gtcgccggca
     2041 ccacggccat gcgccaggcc agcggggcgt atccccgggc gtgctgaacg gcgtggccgc
     2101 attcgtgggc ggcgattccc agtgcggcca ccgagggcgt ccgatacaca tccggcgaga
     2161 ggcagagccg tttttgggaa ggatcgtaat gatcccccag catgctctgg atctcgacga
     2221 cctcgacgtc gtgaatgttc gccgtggcca gaatccgctg cgcggcctgc gcgccggtca
     2281 cgccggaagt cgcggccaca tttctccaat gcttgaaggc gctggaaacc ttccattgcg
     2341 ccaaaaggcc gagcaggaag ggaactccga tcaaaagaat aaagagggtc atgtcaaaaa
     2401 attgggaact tgattgctgt gggattagac cctttgccga tccgcccgtt caatgcaacc
     2461 caaaccgttt ttacagctcc agcggcttcg gcatccggct cagcacgcgg ttccccgcgc
     2521 ccgtgaccag cgcgacatcc tcatgccgca cgcctcccag gccgggaatg taaatcccag
     2581 gctccaccgt gatgacttgg ccgggcttga acgtggtggc gccgaagcgc ggagcttcgt
     2641 ggatttccag accgagcccg tgccccgtgc cgtggaaaaa tccctgccag cggccctcgc
     2701 ggatttcggt cggatatccc cgctccgcaa aaaaggcctt caggccctca tggatcgttt
     2761 tgccagcggc tccgggtttc atttcccgga gcgcgcgctt ttgcccctcc aggcaggttt
     2821 gccagagctt gcgccgcgcc tcgctcaccg ggcccttgac gacggtccgc gtcagatcgc
     2881 cgtggtagcc cgaagccgtg tggcgcggga agacatcgag aataatcagc tcccccgcgc
     2941 gcagcgggcc gctgccgcgc tcgtgcggat cgcaggcctg atcgccgccg gcgacgatgg
     3001 tattctgcgc gttgccgccg agccggagga ttgcgccgtc gatctccgcg cgcagccgtt
     3061 cggaagtcag cacgctcccg ccccagacca gccgccggtc ccgcgggcga atctccgcgg
     3121 ccttcagcac ctcgatgccg cggctcatgc ccgcttcggt gatgcgcagc gcgcgcgtga
     3181 tccacgccgc ctccgccgcg gttttgaatt cgcgttccgg ccagaaactt ccctccacgg
     3241 cttccaggcg gatgccgcgg cgctgcagct ccagcgccag ccccagggga aaatcccccg
     3301 gcacccgcgc ggcggaaacg cgctgggcct tcagccagcc cgccagcgcg gcggcatacc
     3361 ccggctgccc ggcgcgcgcc tcgaattcgc tcagcgagtc cacgcggtcc accgccgcgt
     3421 cccgccttcc ccggtccact tccagatcgc tcaacaacaa aaaccgcccg cgcgaagtct
     3481 gcaaaaacgc aaaggcgtcg ggcgcaaaaa accgggtcgc ataaagcagg tcggcattct
     3541 gctcgcttgc gccgtaaatc agccgggcgg aggatcgggc catgacggaa tagcgtcacc
     3601 attcgcgaaa aaaatcacaa tcttttcctc cgccgggaaa aactttttcc gaactaacgg
     3661 gttgtatttt aaaattaaaa caatatttgt ttgaacaaat ttagagccaa aaagtctaat
     3721 tcccccaaca ttccaaatct ttatgaagca agctgccatg ggcttcagcg gtcgccttct
     3781 cgcaatgacc gctcttttga tattcacgct caacgcccat tcggccgagt tcgcctcgac
     3841 cgcgaagctc attgccacca gcgattgggg ggacggcttc gtggcctcct tggaaatcaa
     3901 gaacacctcc agccagccgg tcaaaaactg gacggcatcc ttcgatcttc cacggcagat
     3961 cgaatccctg tggaatggca cgatcctctc gcacgcgggc aatcgctaca cggtccaaaa
     4021 cgcgggctgg aattccgaaa tcccgcccgg cggctcggtc ctcatcggct tccagggtta
     4081 ccccggcaat ccgccgcaac ccacccgcgt caaattgatg ggcgacggcg ccagcccctc
     4141 gccgactccg acgcccgccc ccactccgac tccctctcct tccgcgacgc ccaagccctc
     4201 ggcaactccg aagccttccg ccaccccgtt gccggctcct tcggccacgc catccgcgac
     4261 cccctcccct tcgccgagcg cgactcccac ccccgcggcc ggcgcgccgg gttaccccag
     4321 cctgtccgtc ctgcgcgact ggcagaatgg cggctacgaa gtgacctgga ccaaatggag
     4381 cgggccgaac gccacgcgct ggcgcc
//
LOCUS       DATDAY010000044         2805 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_2023196, whole genome shotgun sequence.
ACCESSION   DATDAY010000044 DATDAY010000000
VERSION     DATDAY010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2805)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2805)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2805
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2023196"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1417
                     /locus_tag="VIS74_00895"
     CDS             <1..1417
                     /locus_tag="VIS74_00895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5791822.1"
                     /translation="PLALGQVYLEGDLPDNPGYESNSPQELLPALVEPGLSPHAIPSP
                     ATPESAGETGFVPLYRLTRPFYDSIFAQLPPQYLPNRDPSGFRSVTVNLPPIGLQPRA
                     YRLGLLEFYPRFGLSQSFDSNVNLTATNEIADFYLTPKAGLEFQIGTPDSIYLEGYDT
                     MLAARGSYEAYGDLFYGHPELSAFNQQLSLAGRIGRSAAIWRPFLEASDLTGSNLLMA
                     ELVNRTRRIRVLPGIFAEYKFTERTGGNQTFSYYRFQHTDPQYINTQTWRTEQELTYR
                     VTNDLRAILWAEYRYTEPSAGSNGREYFAGAGWAGKPDPRLYSELRIGWDFLDLEGGV
                     PDRQNLSGLRFNGYTTFDWSPRFRPTFRYDRDYVFNEVDENDNYVSTLLQLKGEIFLG
                     GNWYVTPYFGFSIQEFETSRRITLQWRPELEVSYALPSNLTPNQPRIFAKAACMSAQT
                     VKGSGEPIEDLRFSVGFDLKF"
     gene            1495..2076
                     /locus_tag="VIS74_00900"
     CDS             1495..2076
                     /locus_tag="VIS74_00900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5791823.1"
                     /translation="MKKYWAIILAAGLAGCATTSQPPLTAGEPPPMRETHAPYFMAPL
                     DQATLEFRDGSRRIVQINNEGSLDFDGGSKWVKGLPLEEFRGLLRKRYPNVRSIQIEE
                     FRPNRVTVLGEVFHQIHTDLTDGPMRVLDAVAAANGFTPLANKRRVRLLRQNAGAIQV
                     YELDLREMMRGDDLVQNLLLKPGDVISVPRNFL"
     gene            2080..>2805
                     /locus_tag="VIS74_00905"
     CDS             2080..>2805
                     /locus_tag="VIS74_00905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5791824.1"
                     /translation="MPVPSPWEIQSSSQPFSPGLGARDIIRFFIGNLLLLLICLAIGV
                     WLGLLIQQLTPRAYESVGNFIVDELPFNQTSGTVDAETERQLIQTLILSLPSRYMRSA
                     IEKRLALPEGRITFPELGVPLKLDGPEPRANIQITAVRNTRIGQITATSQDPEFAAEI
                     VNAILDEMRIYNLIGGRLKNLRVSLDFSKTQAEAIITQLAEVSAQRLKVEKEKSALDE
                     YTRQGLPLESFPAFSTDATLNNLK"
BASE COUNT          562 a          963 c          727 g          553 t
ORIGIN      
        1 tcccttggca ttgggtcaag tttatctgga gggcgatctc ccggacaatc ccggttacga
       61 atccaactcc ccccaggaat tgcttcccgc gctggtggaa ccgggactca gcccacacgc
      121 gatcccctcg cccgccacgc cggagtccgc gggcgaaacg ggatttgtgc ccctctatcg
      181 cctgacccgg ccattctacg acagcatttt cgcgcagctc cccccgcaat atctgcccaa
      241 ccgggatcct tccggtttcc gctcggtaac cgtcaacctg ccgcccatcg gcctccaacc
      301 ccgggcttac cggctggggc tgctggagtt ttatccgcgc tttggccttt cgcagtcctt
      361 cgactcgaat gtcaacctga ccgccaccaa tgagattgcc gacttctacc ttactcccaa
      421 agccggactg gaattccaga tcggcacccc ggattccatc tacctcgaag gctacgacac
      481 gatgctggcc gcccgcggtt cctacgaggc ttacggagac cttttttatg ggcatcccga
      541 actcagcgct ttcaaccagc agctttcgct cgccggacga atcggccgct ccgccgccat
      601 ctggcgcccg ttcctggagg cctcggacct caccggcagc aatctcctca tggccgagct
      661 cgtcaaccga acccggcgca tccgggtgct ccccgggatt tttgccgaat acaagttcac
      721 cgagcgaacg ggcgggaacc aaaccttcag ctattaccgc tttcaacaca ccgatccgca
      781 atacatcaac acccaaacct ggcgcaccga gcaggaattg acctaccgcg tcaccaatga
      841 cttgcgggcg atcctctggg cggaatatcg ctataccgaa cccagcgccg gctcaaacgg
      901 ccgggaatat ttcgccggcg ccggctgggc gggcaaaccc gatccccggc tgtattccga
      961 actccgaatc ggctgggatt tcctcgatct ggagggcggc gttcccgatc gccaaaatct
     1021 ctccgggctc cggtttaatg gctacaccac cttcgactgg agcccccgtt tccggcccac
     1081 tttccggtat gaccgcgact atgtcttcaa tgaagttgac gaaaacgaca actacgtctc
     1141 gaccctgctt cagctgaagg gcgaaatttt cctgggcggc aattggtatg tcacccccta
     1201 cttcggcttc tccattcagg aattcgaaac ttcccggcgc atcacccttc agtggcggcc
     1261 cgaactggag gtttcttatg ccctgccaag caacctgacg cccaatcagc cgcgaatatt
     1321 tgcaaaagcc gcctgcatga gcgcccagac cgtaaaaggc tccggcgaac ccatcgagga
     1381 tctgcgtttc tccgtggggt ttgatctcaa attttgagcc ttgaaatccc ggcagccgcc
     1441 tgatcttctg aaagataatc aggcggccat ccctccgcgg ccccggaatt tttcatgaag
     1501 aaatattggg ccatcattct cgccgccggg ttggccggtt gtgcaaccac aagccagccg
     1561 cccctcaccg ccggcgagcc gcctcccatg cgggaaaccc acgcgcccta tttcatggcg
     1621 ccgctggatc aggcgaccct cgaattccgg gacggctccc gccggatcgt tcaaattaac
     1681 aacgaaggga gcctggattt tgacggcggg agcaagtggg tgaagggcct gcccctcgaa
     1741 gaatttcgcg ggctgctcag gaagcgttac ccgaacgtcc ggagcatcca gatcgaggaa
     1801 ttccgcccga accgcgtcac cgtgctggga gaggttttcc atcagatcca caccgacttg
     1861 accgacggtc ccatgcgcgt gctcgacgcc gtggcggcgg ccaacggctt tacgccgctg
     1921 gcgaacaagc gccgggtcag gctcctgcgg cagaacgccg gcgccattca ggtctatgag
     1981 ctggatctcc gcgagatgat gcggggggac gacctcgtcc agaatttgct gctcaaaccc
     2041 ggcgacgtga tttccgtgcc gcgcaacttt ttataaacca tgccagtgcc cagtccctgg
     2101 gaaatccaat cgtccagcca gcccttttcc cccggcctgg gcgcgcgcga catcatccga
     2161 tttttcatcg gcaatctcct tttgctcctc atctgcctgg cgatcggcgt ctggctgggc
     2221 ttgctgattc agcaactcac cccgcgcgcc tacgagtcgg tggggaactt tatcgtggac
     2281 gagcttccgt ttaaccagac ttccggaacc gtggacgccg agaccgagcg ccagctcatc
     2341 cagacgctca tcctcagcct gccaagccgt tacatgcgtt cggccattga aaaacggctc
     2401 gctcttcccg aagggcgcat caccttcccc gaattgggcg tccccctcaa gctggacggc
     2461 ccggaacccc gggccaatat ccagatcacc gccgttcgaa acacccgcat tggccagatc
     2521 accgccactt cgcaggatcc ggaattcgcc gccgaaatcg tgaacgccat tctggacgaa
     2581 atgcggatct ataatctgat cggcggacgg ctcaaaaacc tgcgggtcag cctcgatttc
     2641 agcaaaaccc aggccgaagc aatcatcacc cagctcgccg aggtctccgc ccagcggctg
     2701 aaggtcgaaa aggaaaaatc cgcgctggat gaatacacgc ggcagggact gccgctggaa
     2761 tcctttcccg ccttctccac cgacgccacg ctcaacaatc tcaag
//
LOCUS       DATDAY010000045         9180 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_2193799, whole genome shotgun sequence.
ACCESSION   DATDAY010000045 DATDAY010000000
VERSION     DATDAY010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 9180)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9180)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9180
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2193799"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..363
                     /locus_tag="VIS74_00910"
     CDS             <1..363
                     /locus_tag="VIS74_00910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5791825.1"
                     /translation="SASIGCARVGHRQSYAPGFGNKAWGIFFVVFSPEGGAHPTRLEF
                     SFNRIGEIRAEVKRKLAVQPPLTKKSKDFPLAAPPVDSMDSAIPRFAILPGRHPANAE
                     CLKPVPVRNDFPRRSPNE"
     gene            complement(568..1797)
                     /locus_tag="VIS74_00915"
     CDS             complement(568..1797)
                     /locus_tag="VIS74_00915"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEY5791826.1"
                     /translation="MSFQNLGLADAVVHGAQRMGYIDPSPIQEQSIPVILAGRDLIAS
                     AQTGTGKTAAFGLPILSRLQKAKGVIRCLILEPTRELAMQVETAFRDFSFFTDLDAAL
                     IHGGVGYGKQREQLKAGVDVIAATPGRLLDLMQEGDVNLSTVECLVLDEVDRMLDMGF
                     LPDVRRIIEKCPQQRQTLLFSATIPEEIERLSAWCLKDPAKIEIGARRSPAETVTHAF
                     YPVAVAQKFDLLLSILERTDYHSVLIFTRTKEGADRISRQLKDSQHSVAVLHSNRSQG
                     ERVEALEGFKSGKYEVMVATDIAARGIDIAGVSHVINYDVPQHPEDYVHRIGRTGRAQ
                     QDGDAFTLMTAEELPHVKDIERFIGQTVPRQKLENFDYVFTALFDEARVAAVLKGAKG
                     VRTHKGMSFGRAAKRRR"
     gene            complement(1803..2387)
                     /locus_tag="VIS74_00920"
     CDS             complement(1803..2387)
                     /locus_tag="VIS74_00920"
                     /inference="COORDINATES: protein motif:HMM:NF013786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HEY5791827.1"
                     /translation="MNKLSYELTPGRVFLVRGTGEDWRGRVLAWCERLFGLRDLEFAY
                     QEFGKPYFKNHPELAFSVSHTRTALCAAFCVQEIGLDVEFRDRRIRALSLAGRYFHPP
                     ETGYLRGLAEDAHAAAFLSLWTAKEACVKLTGEGIYRGLPQCHVEPSSAPANAVQNGR
                     PAFLRQQVWADGLIATVAAWSEFQLECLEQSFDC"
     gene            2437..2835
                     /locus_tag="VIS74_00925"
     CDS             2437..2835
                     /locus_tag="VIS74_00925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5791828.1"
                     /translation="MDVSNINSSTLQNLVGLIKKKEELELQLGKIEAQIAAAISGKKA
                     GKPGRPALKGKPGRPAKAAPKRSGKRGALKEAITDLLGKAGPEGISAKEISEKLGVPN
                     QHVHVWFGTTGKKITGLTKLAKGVWAVQDK"
     gene            complement(2863..3642)
                     /locus_tag="VIS74_00930"
     CDS             complement(2863..3642)
                     /locus_tag="VIS74_00930"
                     /inference="COORDINATES: protein motif:HMM:NF019887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HEY5791829.1"
                     /translation="MSTDFDGTLIEYQSDGRCVPDLARILTDALREGTIWAVNTGRTL
                     EHLDEGLAQFAAPVSPHFAIVNERYIFRAAARGWESLEPWNSRCTSDHQDLWDQTGGI
                     FRQLARWCRRNGNVELVEVDGQLEGLITADEETMEETVVFLDGLRGQVSDFSWQRNSI
                     YLRFAHRAYDKGSALIHLAVELGIPLDRVIAVGDHYNDLPMLNGTSAGRVACVANAVE
                     EIKTAVLTVGGYVAGAHAGRGVAEALAYFKNQPAAARTAGD"
     gene            3715..3987
                     /locus_tag="VIS74_00935"
     CDS             3715..3987
                     /locus_tag="VIS74_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5791830.1"
                     /translation="MKSAYELAMSRLEKSSPTIALTEAQKRELAEIDSLYTAKIAERR
                     LLLEPEIARSAGNPAERESLRKQLRSEVARLEEECAAKKERVRRAG"
     gene            complement(3984..5660)
                     /locus_tag="VIS74_00940"
     CDS             complement(3984..5660)
                     /locus_tag="VIS74_00940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5791831.1"
                     /translation="MNALKFSLVFLLGTASLLPAAALMPPGPREVDAILTEGFPSAAG
                     RIGALLRENYRPGTGSMPSNAGQPAFRAWYGLGMGCRLFAVDGAGLDPAFEKAVMAHS
                     AFSPDFLPNLAREDDAAAVLKILQELWQADPARFWQYFRLAIALALVFDQPPPADWPH
                     DQTNSAALPQAAMPPAEAFAFWIESDKTGKLYADPKTLGVDQLKFMVDVFLPASELRW
                     AQKNARFPRANFGKAFSSITYDYSRTRTRQYDWPHESYSLEEIRKQGGICVDQAYFAA
                     IAGKANGLPTLFFSGQGTSGGHAWFGYLKGANRWELDCGRYENQNYAVGEALDPQTWQ
                     PINDAELDYLSKPSQRGDAFRQSQIDLGMARLYQENQQPDKALAAVNSAIALAPENVA
                     AWKERTSLLNDPSAQKAHFEKALNQFPANREVRVFFQKELADWHRRQGDAATAEEWER
                     KIISQNRSGRSDLSIAAAAGGLAALVEARKMDEAMKEYRSLLGRLGRKGGGNFYYEIV
                     APFTESLIKAGDSQGAGRALELARRTLQPDSGSILDQEFQTLETRVKAVR"
     gene            complement(5657..6739)
                     /locus_tag="VIS74_00945"
     CDS             complement(5657..6739)
                     /locus_tag="VIS74_00945"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HEY5791832.1"
                     /translation="MSSLPSTDLLALLAEAVRFGASDLHLAADTPPLMRLNGEILPLD
                     ETPLTAEQCSELLFSVLTQNQRARLEEAWELDFALQVETIGRFRGNAHYTRGAVGAAF
                     RLIPAQIPELGALGHRASLFGIAEFQRGLVLVTGITGSGKSTTLASIVQHISRRRRGV
                     IITIEDPIEYVLSNDLCLVRQREIGHDTKTFAEALRHALRQDPDVILVGEMRDPETIS
                     AAITAAETGHLVLATLHTIDAPKAIDRIVDAFPGGQQPQIIAQLANSLEAVVSQRLIL
                     RADNGGRVLASETLIANTAVRACIRERRWEQLVGLIEIGSKDGMNTLEESLASLVKTG
                     QITPEQAEAEARDPDVLRSFLVPRHR"
     gene            complement(6750..7808)
                     /locus_tag="VIS74_00950"
     CDS             complement(6750..7808)
                     /locus_tag="VIS74_00950"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HEY5791833.1"
                     /translation="MSLASLLAAAETSAASDLLLHEGRPARLKIAREWREIEGDPVDA
                     AILDEIWRETQAPADAREYDAALASPAYARFRVNCFRQLGKRGAALRRVRTEIPPMEE
                     LHLPADLLRAWLRRRSGVILVCGPTGSGKSTTLAACLSWLNRTSALHVVTLEDPVEFV
                     FADQGCRFTQRELGIDTGSFAGGLRSALRQSPDAILIGEIRDSETTLSAMQAAETGHL
                     ILSTLHVNRAAEAVERLEYLAGDRDRPLLRALLAANLIGVICQRLIPANDGTTTAALE
                     YFSNQGLVGKLLLEARTAELTDLVEQGDGREALSFNQSFRRLVSDGTLTAETALAHSP
                     APAELQRIFRGISGGSRG"
     gene            complement(7852..>9180)
                     /locus_tag="VIS74_00955"
     CDS             complement(7852..>9180)
                     /locus_tag="VIS74_00955"
                     /inference="COORDINATES: protein motif:HMM:NF022466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2851 family protein"
                     /protein_id="HEY5791834.1"
                     /translation="ESPMTPALHPLPRGLTGYAEALFPAGRVLEERIEPDSEREWQAW
                     FYQGDLGARWPSTDGREVELLDPGFWNREPGPDFTAATIRVDGTALLHGDIELDPHAL
                     DWERHGHATNPAFSKVVLHVFSQPAARTFFTRTSEHRNVLQVALSGPPRPSRNPDQGA
                     VNRKTALRLLEVAVRFRFHEKSRRLRQAVRLHGWPPALDQALARSLGYKRNSLPFLLL
                     AQRCRWDHPHRESAEARLFGTAGFLAAEQFETAPAGARAYLRKLWDQWWALREEGSRL
                     ILPADAWNLAGIRPANHPHRRLGALIAARRRLPEWSEAFRQRNLGRLAGVLGGLEHPF
                     WQTHWNLRGAPLAGRTTAALLGRERLGDFLINAYFPWSLSEDPDGWSAFLKERAPAIP
                     AQIRPLAAWLSPELEPADRRFAWIQQGLLQMAADLRGWKSPRELAEALNF"
BASE COUNT         1696 a         2818 c         3009 g         1657 t
ORIGIN      
        1 agtgcttcta taggttgtgc gagagtaggc catcgccaga gttatgcccc aggttttgga
       61 aacaaagcct ggggcatctt ttttgtcgtt ttttcgccgg aaggcggcgc gcacccaacg
      121 cgccttgaat tttcattcaa ccggatcggc gaaatccggg ctgaagtaaa gcggaagctg
      181 gctgttcagc cgccgcttac gaagaaatcg aaggattttc cgttggccgc tccgccggtt
      241 gattcaatgg attcggcgat tcctcggttt gcgattttgc cagggcgtca tcccgccaat
      301 gcggaatgct taaagccagt tcctgttcga aacgattttc caaggcggtc gcccaatgaa
      361 tgatttcagc cataattgat aaacgcggac ggcctaatct cgcgcatggc gctgaaatat
      421 ccacttattt gatgcgatcc gttgatcgcc aggctttctg gagcatccgg ggcttcccgg
      481 gctcggccgg cgccataatc atccccggct ctgggaaatg ccggggtttt ttaaacttct
      541 gggggaatgt gggggggctg aaaaccgtta acggcggcgc ttggcggccc gtccgaaact
      601 catgccctta tgtgtgcgga cgcccttggc gcccttgagc accgccgcga cccgggcctc
      661 gtcaaacagc gcggtgaaga cgtagtcgaa attctccagc ttttgccgcg gaacggtctg
      721 gccgatgaag cgttcgatat ccttcacgtg gggcaattct tccgcggtca tcaaggtaaa
      781 ggcgtccccg tcctgctggg ctcgcccggt ccggccaatg cggtggacgt aatcctccgg
      841 atgctgcggg acatcgtagt taatcacatg gctcaccccg gcgatatcaa ttccccgcgc
      901 ggcgatgtcc gtggcgacca tgacctcgta tttgccgctc ttgaaacctt ccagcgcctc
      961 gacgcgctca ccctggctgc ggttggaatg gagcaccgcc accgagtgct ggctgtcttt
     1021 taactgccgg gagatccgat ccgcgccttc cttggtgcgg gtgaagatca gcacgctgtg
     1081 ataatccgtg cgttcgagga tggagaggag caggtcgaat ttttgcgcga cggcgacggg
     1141 ataaaacgca tgcgtgacgg tttccgccgg cgaacgccgc gcgccgattt cgatctttgc
     1201 cggatcttta agacaccagg ccgaaagccg ttcgatttcc tccgggatgg tcgctgaaaa
     1261 caaaagcgtc tgccgttgct gggggcattt ttcaatgatt cgccgcacgt ccggaaggaa
     1321 gcccatgtcg agcatccggt ctacctcatc gagcacgagg cattccacgg tggataaatt
     1381 gacatcgcct tcctgcatca gatcaagcag gcgtcccggg gtggccgcga tcacgtccac
     1441 tcccgccttc agctgttccc gctgtttgcc gtaacccacg ccgccgtgaa tcaaggcggc
     1501 atccagatcc gtaaaaaagc tgaagtcgcg aaacgcggtt tccacctgca tggccagctc
     1561 gcgcgtgggt tccaaaatca ggcagcgaat cacccccttt gctttctgca gccgtgacag
     1621 gatgggaagg ccgaaggcgg cggttttgcc ggtgccggtt tgggccgagg caatcaagtc
     1681 ccgcccggca aggatcacgg gaatcgactg ctcttgaatg ggactcggat cgatgtaacc
     1741 cattcgttgc gcgccatgca ccacggcatc ggctaaacct aaattttgaa aagacacagg
     1801 gttcaacaat cgaacgattg ttcgaggcat tcaagctgga attcactcca ggccgccacg
     1861 gtggcaatta gaccgtcggc ccagacttgc tggcgcagga aagccggccg gccattttga
     1921 acggcattgg ccggcgcgga ggacggttca acgtggcatt gaggcaggcc gcgataaatt
     1981 ccctctcccg taagtttcac gcaggcctct ttggcggtcc ataagcttaa gaatgcggcg
     2041 gcgtgagcgt cttccgccag gccgcgcaga taaccggttt ccggcggatg aaaataacgc
     2101 ccggccaggc tgagcgcgcg aatccgccgg tcgcgaaatt ccacatccag cccgatttcc
     2161 tggacgcaaa atgcggcgca aagggccgtc cgggtatggg aaacgctgaa tgccagctct
     2221 gggtgatttt taaaataagg ctttccgaat tcttggtaag cgaattccag gtcgcgaagg
     2281 ccgaaaagcc gctcgcacca ggccagcacc cggccgcgcc agtcctcgcc ggttccgcgg
     2341 acaaggaaca cccggcccgg cgtcaactca tagcttagtt tgttcaaaat aatggcttgc
     2401 taataaagaa taaggcatgc acttattcaa agttctatgg acgttagcaa tatcaattct
     2461 tcaaccctgc aaaacttggt ggggctcatc aagaaaaagg aagagctgga gctccagctt
     2521 ggcaaaatcg aggcgcagat cgccgccgcg atttccggta aaaaagccgg caagccgggc
     2581 cgcccggcgc tcaaaggcaa gcccggccgt ccggccaagg ccgctcccaa gcgaagcggc
     2641 aaacgcggcg cgctcaaaga agcgattacc gacctgctcg gaaaagcggg cccggaggga
     2701 atctccgcca aggaaatttc cgagaagctg ggcgttccca atcaacacgt gcatgtttgg
     2761 ttcggcacga cgggtaaaaa aatcacgggt ctgaccaagc tggccaaggg cgtttgggcc
     2821 gtccaggaca aataaaaccc gccgttgctt ttgagggttc ggtcaatcgc cggcggttcg
     2881 cgccgccgcc ggttgatttt tgaaataagc cagggcttcc gcgacgccgc ggcccgcgtg
     2941 cgcgccggcg acgtagccgc cgacggtgag caccgcggtt ttgatctcct ccacggcatt
     3001 cgccacgcag gccacgcgcc cggcggaagt gccgttgagc atggggagat cattgtaatg
     3061 gtctcccacg gcgatcacgc ggtcgagcgg aatgccgagt tcgacggcga ggtggataag
     3121 cgccgagcct ttgtcgtagg cgcggtgcgc gaagcgcagg taaatcgaat tgcgctgcca
     3181 ggaaaaatcg gacacctgcc cgcgcaagcc gtccagaaat accacggttt cctccatggt
     3241 ttcctcatcc gcggtaatca aaccttcgag ctggccgtcc acctccacca gctccacgtt
     3301 gccgttgcgc cggcaccacc gcgccagctg gcggaaaatg ccgcccgttt ggtcccagag
     3361 atcctgatgg tcggaagtgc agcgggagtt ccagggctcc agcgattccc agccccgcgc
     3421 cgcggcccgg aaaatgtaac gctcgttgac aatggcgaaa tggggggaca ccggcgcggc
     3481 aaactgcgcg agaccctcgt cgaggtgctc cagcgtgcgg ccggtgttca ccgcccagat
     3541 cgttccctcc cgcaatgcgt cggtgagaat tcgggccagg tcgggcacgc agcgcccgtc
     3601 ggattgatat tcgatcaggg tgccgtcgaa atccgtgctc aaaagttcca tgcccttgaa
     3661 ttacgcacaa aaacggactt tttcacaagg gcgcgacgcg gtaagtaaag tcccatgaaa
     3721 tccgcctacg agctggccat gagccggctg gaaaaaagtt cccccacgat cgcgctcact
     3781 gaggcgcaaa aacgcgagct ggccgaaatt gattcgcttt acaccgcgaa aattgccgaa
     3841 cgcagattgc tgctcgaacc ggagatcgcc cgatccgccg ggaatcccgc cgagcgggaa
     3901 tccctgcgga agcagcttcg ttccgaggtg gcgcggctcg aggaagagtg cgcggcgaag
     3961 aaggaacgcg tccggcgcgc cggttaacgc accgctttga cgcgggtttc gagcgtctga
     4021 aattcctggt cgaggatgct gccggagtcc ggctgcagcg tccggcgcgc cagttccagc
     4081 gcccggcccg cgccttggga atccccggcc ttgatcaggg attcggtaaa gggcgcgacg
     4141 atctcgtaat aaaaattgcc gccgcccttc cgcccgagcc gtccgagcag gctgcggtat
     4201 tccttcatgg cttcgtccat tttccgcgcc tccaccagcg cggccaggcc cccggcggcg
     4261 gcggcgatgc tcaggtcgga gcgcccgctg cggttttgcg agatgatctt gcgctcccac
     4321 tcctccgccg tggccgcatc gccctgccgg cggtgccagt ccgccagctc cttctggaaa
     4381 aagacgcgga cttcgcgatt ggcgggaaat tgattgagcg ccttttcgaa atgcgctttc
     4441 tgggccgacg ggtcgttgag aagcgaggtt cgttccttcc acgccgccac attttccggc
     4501 gccagggcga tcgcgctatt caccgcggcc agggccttgt ccggctgctg gttttcctga
     4561 tagaggcgcg ccatgccgag atcgatctgg gattgccgga aggcgtcgcc gcgctgggaa
     4621 ggtttggaaa ggtagtcgag ttccgcgtcg ttgatcggct gccacgtttg cgggtcgagc
     4681 gcctcgccga ccgcgtaatt ctggttttcg tagcggccgc agtccagctc ccagcgattg
     4741 gcgcccttca ggtagccaaa ccacgcgtgg ccgccgctgg ttccctggcc ggagaaaaac
     4801 agggtgggca gcccgttggc cttgcccgcg atcgcggcga agtaagcctg gtccacgcag
     4861 atgccgccct gcttgcggat ttcctcgagc gaataagatt cgtgcggcca gtcgtattgc
     4921 cgcgtccggg tgcgggagta atcataagtg atcgaactga atgcctttcc aaaattcgcc
     4981 cgtgggaacc gggcgttttt ctgggcccag cgcagttcgc tggcgggaag aaacacgtcc
     5041 accatgaact tgagctgatc cacgcccagc gttttcgggt ccgcgtaaag ttttcccgtc
     5101 ttgtcggatt cgatccagaa ggcaaacgcc tccgcgggcg gcattgccgc ctgggggagg
     5161 gccgcggagt tcgtctggtc gtgtggccaa tcggccggcg gcggctggtc gaaaaccagc
     5221 gcgagggcga tggccaggcg gaagtattgc cagaatcgcg cgggatcggc ttgccagagt
     5281 tcctgcagga ttttcaggac ggcggcggca tcgtcctccc ttgccaggtt cggcaaaaaa
     5341 tccggggaga acgcggaatg agccatcacc gccttttcaa aggccggatc cagcccggcg
     5401 ccatccacgg cgaataggcg gcaccccatt ccaagcccat accaggcgcg aaaggcgggc
     5461 tggccggcgt tgctcggcat gctgccggtt cccggtctgt agttctcccg gagcagtgcg
     5521 ccgatccgtc ccgccgcgga ggggaacccc tcggtgagaa tggcatccac ttcccgcggg
     5581 cccggcggca tgagggcggc tgccggaagc agggaggccg tgccgagaag gaaaaccagg
     5641 ctgaatttca gcgcgttcat cggtggcgcg gcacgagaaa gctgcgcaga acgtccggat
     5701 cgcgcgcttc ggcttccgcc tgttccgggg tgatctggcc ggtcttcacg aggctggcca
     5761 gggattcctc gagggtgttc atgccatcct tgctcccgat ttcaatgagc ccgacgagct
     5821 gctcccagcg gcgctcgcgg atgcaggccc ggacggcggt attcgcgatg agcgtctcgc
     5881 tcgcgagaac gcgcccgcca ttgtccgcgc gcaaaatcaa tcgctgggaa acgacagctt
     5941 ccaggctgtt ggccagctgg gcgatgatct gcggctgctg gccgccgggg aacgcgtcca
     6001 caatccggtc gatggctttg ggcgcgtcga tggtgtgcag cgtcgcgagc acgaggtggc
     6061 cggtttcggc ggcggtaatg gcggccgaga tggtctcggg atcgcgcatt tcacccacga
     6121 ggatgacgtc cggatcctgc cgcagcgcat ggcgcagcgc ctcggcgaag gttttggtat
     6181 cgtgcccgat ctcccgttgc cggacgaggc agagatcatt cgagagcacg tattcgatgg
     6241 ggtcttcgat ggtgatgatg accccgcgcc ggcggcggct gatgtgctgg acgatcgaag
     6301 ccagcgtggt gcttttcccc gagccggtga tgccggtcac gagcacaagc ccgcgctgga
     6361 attcggcgat gccgaaaagg ctggcgcggt ggcccagcgc gccgagttcc ggaatctggg
     6421 ccgggatgag ccggaaggcc gcgccgacgg cgccgcgcgt gtaatgggca ttcccccgga
     6481 agcgtccgat cgtttccacc tgtagggcga aatccagctc ccaggcttcc tccaggcgcg
     6541 cgcgctggtt ctgggtgagc acggagaaca gcagctcgct gcattgctcg gcggtgaggg
     6601 gcgtttcgtc cagcgggagg atctccccgt tcaaacgcat cagcggaggg gtgtccgcgg
     6661 ccaagtgcag gtccgaggcg ccgaatcgca cggcttccgc aagcagcgcc aggagatccg
     6721 tggagggaag ggatgacatg atggatcaat taaccgcggc ttccgccgga aatcccgcga
     6781 aaaattcgct gcagttccgc gggggcgggc gaatgggcga gcgcggtctc cgcggtcagg
     6841 gttccgtcgg agaccagccg ccggaaggat tggttgaagg aaagcgcctc gcgtccgtcg
     6901 ccttgctcga cgaggtcggt tagctcggcc gtgcgggctt ccagcagcag cttgccgacc
     6961 aacccctgat tggaaaaata ctccagggcc gccgtggtcg tgccatcgtt tgccggaatc
     7021 aagcgctggc agatgacgcc gatcaggttg gcggcaagca gggcgcgcag gagcgggcgg
     7081 tcccggtccc cggcgaggta ttccagccgt tccaccgcct cggcggcgcg attgacgtgc
     7141 agcgtgctga gaatgagatg gcccgtctcc gcggcctgca tggcggaaag cgtggtttcg
     7201 gaatcccgga tttcgccgat gaggatggcg tccgggctct gccgcagggc gctgcgcagg
     7261 ccgcccgcga agctgccggt atcgatgccc aactcgcgct gggtgaagcg gcagccctgg
     7321 tcggcgaaca cgaactcgac gggatcttcg agggtgacca cgtgcagggc ggaggtccgg
     7381 ttgagccagg aaaggcaggc ggccagcgtc gtggatttgc ccgaaccggt cgggccgcag
     7441 accagaatca ccccgctccg gcgccgcagc caggcgcgga gcaggtccgc ggggaggtga
     7501 agttcctcca tgggcgggat ttccgtccgc acgcggcgca gcgccgcgcc gcgtttgccc
     7561 agctggcgga agcagttgac ccggaagcgc gcgtaggcgg gggaggccag cgcggcgtcg
     7621 tattcgcgcg catccgccgg ggcctgcgtc tcccgccaga tttcgtcgag gatcgcggca
     7681 tcgaccggat caccctcgat ctcgcgccat tcccgcgcga tcttcaaccg cgccgggcgg
     7741 ccttcgtgca gcagcaaatc cgacgccgcg ctcgtttccg cggcggcgag gagagacgcg
     7801 agggacatgc gcccatagaa tcacgaagca ggcggagcac acgccgaaaa atcagaaatt
     7861 cagcgcctcg gcgagctcgc gcggggattt ccagccgcgg aggtcggccg ccatttggag
     7921 cagcccctgt tgaatccacg cgaatcgccg gtccgccggt tccagttcgg ggctgagcca
     7981 tgccgccagc gggcggattt gggcgggaat ggccggcgcc cgctctttca gaaaggcgct
     8041 ccatccgtcc ggatcctcgc tgagcgacca ggggaaatag gcattgatca ggaaatcccc
     8101 cagccgctcc cggcccagca gggcggcggt ggttcgcccc gccagcggag cgccgcgcag
     8161 gttccaatgg gtttgccaaa acggatgttc cagcccgccg agcacgccgg ccagccggcc
     8221 caggttccgc tgccggaagg cttcgctcca ctccggaagc cgccgccggg cggcgatcag
     8281 cgcgccgagg cggcggtgcg gatgattggc cgggcggatg ccggcgaggt tccaggcatc
     8341 ggcgggcagg atgaggcggg agccttcttc gcgaagcgcc caccattgat cccataattt
     8401 ccggagatac gcgcgggcgc cggccggggc ggtctcgaac tgttccgcgg ccagaaagcc
     8461 ggcggttccg aaaaggcgcg cctcggcgga ttcgcggtgg ggatgatccc agcggcaccg
     8521 ctgggcgagc agcaggaagg gcagggagtt gcgtttatag ccgaggctgc gggccagggc
     8581 ctgatccaaa gccggcggcc agccgtgaag ccgcacggcc tggcgcagcc gccgggattt
     8641 ttcatggaac cggaagcgga cggccacctc caacagccgg agcgccgttt tgcggttcac
     8701 ggctccctga tccgggttcc gggacgggcg cggcggaccg gacagggcca cctgcagcac
     8761 gttgcgatgc tcggaggtcc gggtgaaaaa agtccgggcg gccggctggg aaaagacgtg
     8821 aagcacgacc ttggaaaaag cggggttggt ggcatggccg tgccgttccc aatccaaggc
     8881 gtgggggtcg agttcgatgt caccgtgaag cagggcggtt ccgtccaccc ggatcgtcgc
     8941 ggcggtgaaa tccggacccg gctcgcggtt ccagaagccg ggatcgagca attccacctc
     9001 ccgcccgtcc gtgctcggcc agcgcgcgcc gaggtcgccc tggtaaaacc acgcctgcca
     9061 ttcccgttcg gaatccggtt cgatcctttc ctccaaaacc cggccggcgg ggaagagggc
     9121 ttcggcatat cccgtgagtc cccggggcag cggatggagg gcgggcgtca tcggggattc
//
LOCUS       DATDAY010000046         2039 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_2406550, whole genome shotgun sequence.
ACCESSION   DATDAY010000046 DATDAY010000000
VERSION     DATDAY010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2039
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2406550"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..958
                     /locus_tag="VIS74_00960"
     CDS             <1..958
                     /locus_tag="VIS74_00960"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEY5791835.1"
                     /translation="GPALAAGCTMIAKPASSTPYSAFALAVLAERAGIPSGVLNVLTG
                     NSKEIGGEMTSNPDVRKITFTGSTEVGRLLMKQSSSTIKKLSLELGGNAPFIVFDDAD
                     LDAAVEGAIISKYRNAGQTCVCANRLYVQAGVYDAFAEKLVAAVGKLKVGNGFEPGVQ
                     QGPLIDNAAVEKVEEHIQDAVTKGGHVVLGGKRHALGQTFFEPTVLTGVTTQMKVAKE
                     ETFGPLAPLFRFETDEEGIQMANDTEFGLASYFYSRDLGRVWRAAEALECGMVGINTG
                     LISNEVAPFGGVKQSGLGREGSHYGLEDFLEIKYLCFGGLDK"
     gene            986..>2039
                     /locus_tag="VIS74_00965"
     CDS             986..>2039
                     /locus_tag="VIS74_00965"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEY5791836.1"
                     /translation="MQDQDLFRQQAYIGGRWCDADSGATLEVNNPATGDILGTVPKMG
                     ANETWRAIEAAKDAFVDWSRKPAKERSILMRRWHDLIMANVEDLGALMTAEQGKPLTE
                     AKGEVAYAASFIEWFAEEAKRVYGDTIPSPWNDRRLVVIKQPIGVCAAVTPWNFPAAM
                     IARKAAPALAAGCTMVAKPAESTPYSAFALAVLAERAGIPSGVLNVLTGEAREIGGEM
                     TSNPDVRKITFTGSTEVGRLLMKQSSSTIKKLSLELGGNAPFIVFDDADLDAAVEGAI
                     ISKYRNAGQTCVCANRLYVQAGVYDAFAEKLVAAVGKLKVGNGFEPDVQQGPLIDYAA
                     VEKIEEHIQDAVSKGGH"
BASE COUNT          418 a          580 c          633 g          408 t
ORIGIN      
        1 tggcccggcg ctggccgcgg gatgcacgat gatcgccaag ccggccagtt ccacccctta
       61 ctcagcgttc gcgcttgccg tgctggcgga acgcgccggg attccctccg gcgttctgaa
      121 cgtgttgacc ggcaattcga aggagattgg gggcgaaatg acctccaatc ccgatgttcg
      181 caagattact tttacgggtt caacggaggt tggccgcctg ctcatgaagc agagctcctc
      241 caccatcaag aagctttccc tggagctcgg cgggaatgca ccctttatcg ttttcgatga
      301 tgccgatttg gatgcggccg tcgagggggc gatcatttcc aagtatcgca acgccgggca
      361 gacctgcgtt tgcgcgaacc ggctctacgt gcaggccggc gtgtacgacg cctttgccga
      421 aaaactggtc gccgcagtcg gaaaactcaa ggtcggcaat ggctttgagc cgggagttca
      481 gcaggggccc ctcatcgata atgccgccgt ggagaaggtg gaggaacaca tccaagacgc
      541 cgtgacgaag ggcggccatg tcgttctcgg aggcaaacgg catgcgcttg gccagacctt
      601 ttttgaaccg accgtgttga ctggtgttac gacgcaaatg aaggtcgcca aggaagaaac
      661 cttcggtccg ctcgctcctt tgttccgctt tgaaacggac gaggaaggta tccaaatggc
      721 caatgatacg gaatttggct tggccagcta cttctatagc cgcgatctcg gccgtgtgtg
      781 gcgggcggca gaggctctcg aatgcggaat ggttggcatc aataccggct tgatttcgaa
      841 cgaagtcgcc ccgtttggcg gggtcaaaca gtccggactt ggccgggagg gctctcacta
      901 cggtttggag gacttcctcg agatcaaata cctgtgcttc ggcgggctcg acaaatgagc
      961 aagacgtaga atgacggcat tttctttgca agatcaggac ctcttccgcc agcaagctta
     1021 catcggtggg cgctggtgcg atgccgacag cggagcgaca ctggaagtca ataatcccgc
     1081 aaccggggac atccttggca cggtgcccaa gatgggcgcc aacgaaacgt ggcgcgcgat
     1141 tgaggccgcg aaggacgcct ttgtggattg gagtcgaaaa cctgccaagg agcgaagcat
     1201 tctcatgcgc cgttggcatg acttgatcat ggccaacgtt gaggatctcg gcgcgctgat
     1261 gactgccgag caaggcaaac cgctgaccga agcgaagggt gaagtcgcct atgccgcttc
     1321 ctttatcgag tggttcgccg aggaagccaa gcgggtttac ggggacacga tcccctcgcc
     1381 ttggaatgat cggcgcctcg tcgtgatcaa gcagcccatc ggcgtctgtg cggccgtcac
     1441 tccgtggaac tttccggccg ccatgatcgc gcgaaaggct gcaccggctc tggccgccgg
     1501 atgcacgatg gtggcgaaac ccgccgagtc gactccatac tcggcttttg cgctcgcggt
     1561 tttggcggaa cgcgccggga ttccctccgg cgtcctcaac gtgctgaccg gcgaagccag
     1621 agagattggg ggcgaaatga cctccaatcc cgatgttcgc aagattactt ttacgggttc
     1681 aacggaggtt ggccgcctgc tcatgaagca gagctcctcc accatcaaga aactttccct
     1741 cgagctcggc gggaatgcgc cctttatcgt tttcgacgat gccgatctgg acgcggccgt
     1801 ggagggggca atcatttcca aatatcgcaa cgccgggcag acctgcgttt gtgcgaaccg
     1861 gctctacgtg caggccgggg tttacgacgc cttcgccgag aagctggtcg ccgcagtcgg
     1921 aaaactcaaa gtcggcaacg gctttgagcc cgatgttcag cagggaccct tgatcgatta
     1981 cgcggccgta gagaaaatcg aggaacacat ccaggacgcc gtctcgaagg gcggccacg
//
LOCUS       DATDAY010000047         2348 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_2490632, whole genome shotgun sequence.
ACCESSION   DATDAY010000047 DATDAY010000000
VERSION     DATDAY010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2348)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2348)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2348
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2490632"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..384
                     /locus_tag="VIS74_00970"
     CDS             <1..384
                     /locus_tag="VIS74_00970"
                     /inference="COORDINATES: protein motif:HMM:NF013044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEY5791837.1"
                     /translation="KKKESGLFLIFPCFLSFVLPDRVVWYRVEPMGPHRMKLLTTMLV
                     PEAATRHEHFAKMMESETKMLIDFHLEDMEMCTAIQRGLSSLGAQRGRLSHLEMSVWL
                     IQRYLAARARGTWPALDRPPAPSQG"
     gene            complement(495..1271)
                     /locus_tag="VIS74_00975"
     CDS             complement(495..1271)
                     /locus_tag="VIS74_00975"
                     /inference="COORDINATES: protein motif:HMM:NF025086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HEY5791838.1"
                     /translation="MNSSIILSQSTKDLSMDRRHFLKILSGGATFLGLSGSLFAGVDP
                     KSSLVNWGRLKFPLAGSDQMDWGVHPQGDLNLIDYLTANTSLQLPNNWNVADVWRLDE
                     MVQFPFLYMHAEDVPVLDSVARANLREYLLRGGFIFAEDCVIGRGTHGHNSRNDFFFR
                     SMVEQLPAIMPEAKLERLPNDHPVFHVLYHLDNGMPHMQGIPHGLHALTLHGRVLAFI
                     SPSDLHCGWTNGYKWFGAEQTQQALRMGTNIYVFAMTQSA"
     gene            complement(1268..>2348)
                     /locus_tag="VIS74_00980"
     CDS             complement(1268..>2348)
                     /locus_tag="VIS74_00980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5791839.1"
                     /translation="LDFTRQGGSVLFALSDAEAAQGFAGSTLEEMLPVHFQPQERASR
                     EDAISREFQERMRQTRGGSNGAAERDFAAGQADRKLDDPTLPFAFSGQTPLADLLRSA
                     QTGQIVPEYQSAAHIYDAKPAAQVLAVAGGNSSEPRPILLATQPFGYGRSAVLATDLL
                     WRWSLKQPSTSTNVETFWQQFVLWLAQPGRRGIRFLEASPEASTRQPAHLVISGMPDP
                     AKAALTAKAPSGKSVSIPLASRSDSALTGEFLPDETGEWLIKAESPAGGLAEKFIRVA
                     EKPLTGETHIQATDRTTLAQIAAATGGKIYDRHAAPFREPAAGAAPLILSQNSRLLWN
                     SWLVFLPLLSLFGAELLLRRYWKLL"
BASE COUNT          418 a          690 c          807 g          433 t
ORIGIN      
        1 aaaaagaagg aatcgggcct gttcctgatc tttccctgct tcctgagttt cgtgctgccg
       61 gatcgcgtcg tctggtatcg ggtggagccg atgggaccgc atcgcatgaa gctgctcacc
      121 acgatgctgg ttcctgaggc cgcgacccgg cacgagcatt ttgccaaaat gatggaatcc
      181 gagacaaaga tgctcatcga tttccatctc gaagacatgg aaatgtgcac cgccatccag
      241 cgcggcctct cctccctggg cgcgcagcgg gggcggttga gccatctcga aatgtcggta
      301 tggctgatcc agcgttacct ggccgcccgc gcccgcggca cctggcccgc gctggaccgc
      361 ccgcccgcgc cttcgcaggg atgacgcgtg ttttcctgcg cgataaagcg gcggtgattc
      421 atctcacctc gcggggcggc tggcacaacc gctcccctat catccgtgtt gatcggtgtc
      481 catccgtggt tcaatcacgc gctttgcgtc atggcgaaga cgtagatgtt ggtccccatc
      541 cgcagggcct gctgcgtctg ctcggcgccg aaccatttat acccgttggt ccagccgcaa
      601 tggagatcgg aaggactgat gaaggccaga acccggccgt ggagggtcag cgcgtgcagc
      661 ccgtgcggaa tgccctgcat gtggggcatt ccgttgtcca ggtgatacag gacgtgaaag
      721 accggatggt cgttgggcag gcgctccagc ttggcttcgg gcatgatggc gggcagctgt
      781 tccaccattg accggaagaa aaagtcgttg cggctgttat ggccgtgggt gccgcggccg
      841 atcacgcaat cctcggcgaa aataaacccg ccgcgcagca ggtattcccg gaggttggcg
      901 cgggcgacgc tgtcgagcac cggcacgtcc tcggcgtgca tgtagagaaa ggggaattgc
      961 accatctcgt cgagcctcca gacatcggcg acgttccaat tgttgggcag ctgcaggctg
     1021 gtattggccg tgaggtagtc gatgagattg aggtcgccct ggggatgaac gccccagtcc
     1081 atttgatccg agccggccag gggaaacttg agccgtcccc agttgacgag cgagcttttg
     1141 ggatcgacgc cggcgaagag cgagccgctc aatccgagga aggtggcgcc cccggaaagg
     1201 atcttgagga aatgacggcg gtccatggac aggtccttgg tcgattggct aaggatgata
     1261 gaagaattca taataatttc cagtatcgtc tcaggagaag ttccgcgccg aagagggaca
     1321 ggagcggaag gaagacgagc cagctgttcc acagcaggcg gctgttttgc gacaaaatca
     1381 agggagccgc cccggccgcc ggttcccgga aaggcgcggc gtgccggtcg taaattttcc
     1441 cgccggtcgc cgcggcgatc tgggccagcg ttgtccggtc ggtggcctgg atgtgcgtct
     1501 ccccggtaag cggcttttcg gcgacgcgaa tgaatttttc ggccaatccg ccggccggac
     1561 tctcggcttt gatcagccac tcgccggttt cgtcgggtaa aaattcgccg gtgagggccg
     1621 aatcgcttcg cgaggcgagg ggaatggaaa ccgatttgcc ggaaggcgcc ttggcggtga
     1681 gggccgcctt tgccgggtcc ggcatgccgc tgatcaccaa atgagcgggc tgtctggtcg
     1741 aggcctccgg gctggcttcc agaaaccgga tgccccggcg gcccggctgg gccagccaaa
     1801 gcacgaattg ctgccagaag gtttccacat tcgtcgaggt gctgggctgc ttgagcgacc
     1861 agcgccaaag cagatcggtg gccagcacgg cgctgcggcc gtaaccgaaa ggctgggtgg
     1921 cgagcagaat ggggcgcggt tcggaggagt tgcccccggc cacggcgaga acctgggcgg
     1981 cgggtttggc atcgtagatg tgcgcggcgc tctggtattc gggcacgatt tgcccggttt
     2041 gggccgagcg cagcaggtcg gccagcggcg tctggccgga gaaggcaaag ggcagggtcg
     2101 ggtcgtcgag cttgcggtcc gcctggccgg cggcgaagtc ccgctcggcg gcgccattcg
     2161 acccgccccg ggtctgccgc atgcgttcct ggaactcccg tgaaatggca tcctcccggc
     2221 tcgcgcgctc ctggggctgg aaatggacgg gcagcatctc ttccagggtg gagccagcga
     2281 agccctgcgc cgcctcggcg tcggacaggg cgaagagcac ggaaccgccc tggcgcgtga
     2341 agtccagg
//
LOCUS       DATDAY010000048         2518 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_94742, whole genome shotgun sequence.
ACCESSION   DATDAY010000048 DATDAY010000000
VERSION     DATDAY010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2518)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2518)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2518
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_94742"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..312
                     /locus_tag="VIS74_00985"
     CDS             <1..312
                     /locus_tag="VIS74_00985"
                     /inference="COORDINATES: protein motif:HMM:TIGR01550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HEY5791840.1"
                     /translation="VRDENLLESALARPQQLFHYGNPTMPELAAAYAAGIVKNHPFLD
                     GNKRTGFMMGAGFLERNGFEFHATEAEAVLRTLALAAGEMPEAAYADWMEANSVRKPA
                     G"
     gene            495..680
                     /locus_tag="VIS74_00990"
     CDS             495..680
                     /locus_tag="VIS74_00990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5791841.1"
                     /translation="MLPSVQFSGFCPAHLQQLGGDADRDFFRLAPAAGGEKILLNEAV
                     TPIPWRGFHGTSGFIYV"
     gene            652..900
                     /locus_tag="VIS74_00995"
     CDS             652..900
                     /locus_tag="VIS74_00995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5791842.1"
                     /translation="MARAALFMFEKIFEGHSDIFCDLAHKKGGDISALVIRDSGLPSI
                     GMFELAMGTFLSNQFKFEIAQYSHHLSRFENGNTAHDA"
     gene            complement(897..1103)
                     /locus_tag="VIS74_01000"
     CDS             complement(897..1103)
                     /locus_tag="VIS74_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HEY5791843.1"
                     /translation="MHAFTAVVERCSETGLFVGFVPGFPGAHTQGESLDELRENLKEV
                     VSMLLEDGEPNLEAEYIGTQTVTV"
     gene            complement(1168..1947)
                     /gene="xdhC"
                     /locus_tag="VIS74_01005"
     CDS             complement(1168..1947)
                     /gene="xdhC"
                     /locus_tag="VIS74_01005"
                     /inference="COORDINATES: protein motif:HMM:TIGR02964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0009115 - xanthine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase accessory protein XdhC"
                     /protein_id="HEY5791844.1"
                     /translation="MHSLTALLPQMTALDQAGEAFVGVTLITARGSVPQEVGAKMLVT
                     AAGRAGGTIGGGRVEEAVIRHARGLLETAPPSPCETVEWNLQRDIGMTCGGVVTFLFE
                     SFRPSAWRIFVFGAGHVSQALARLLATLECRVRVVDSRPEWLNQLPQAANLQTQLAER
                     PEEIVSEIPDGAFVVIMTQGHRTDLPILERILKTRTFPYLGVIGSASKAATLRKELRE
                     AGVAEDSLKAFRCPIGLPLGKDTPEEIAVSIAAELLQVRGT"
     gene            complement(1961..>2518)
                     /locus_tag="VIS74_01010"
     CDS             complement(1961..>2518)
                     /locus_tag="VIS74_01010"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HEY5791845.1"
                     /translation="SLPWLGGQRLLPWMMESLAANGWQPLVVTGPEYFDSWSRELGGE
                     RVALNPHPEAGKTTSLAAGISRLPARAEWILLTSVDQPRLPGLYLRLRRAVPPPGGQT
                     LVVPDWAGRRGHPVVLAASLLPELRALDEDSRGLRGFLDRHDSELMRMPGEPPGSLDW
                     DVNTPEAYQKALAYFREPLAAAPKI"
BASE COUNT          496 a          727 c          797 g          498 t
ORIGIN      
        1 gtgcgcgacg aaaacctgtt ggaatccgct ctggcccggc cgcaacagct ttttcactat
       61 ggcaacccga cgatgccaga actcgcggcg gcttacgcgg cgggaattgt gaagaatcat
      121 ccctttcttg atggcaacaa gcgtaccggg ttcatgatgg gagccggttt tctcgaacga
      181 aacggcttcg agttccatgc gacggaagcg gaagccgtgc tgcgcacgct ggcgctggcg
      241 gctggcgaga tgccggaggc cgcttatgcg gattggatgg aggcaaattc agttcggaaa
      301 ccggcaggct gagatttttt gaacagaagg gagcaaagaa agcaaagggg ggatttgaat
      361 ccaatccttt gcgagctttg cggccttctg tttcataagt ttccccgaag gtttttgcgg
      421 ggaacgattg attttttgag cagaaagaag atcacaaagg acacaaaggg acgaaaatcc
      481 gggctttgct ttctttgctc ccttctgttc aattttcagg gttttgtccc gcgcacctgc
      541 agcagctcgg cggcgatgct gatcgcgatt tcttccggct tgcccctgcg gccggcggcg
      601 agaagattct cttaaacgaa gctgtgacgc cgatcccttg gcggggattc catggcacga
      661 gcggctttat ttatgtttga gaaaatcttc gagggtcatt ccgatatctt ttgcgatttg
      721 gcgcacaaga aagggggaga tatctctgcc cttgtgatca gggacagtgg tttgccgtcc
      781 atcgggatgt ttgaactggc aatgggaacc tttctgtcga accagttcaa atttgagatt
      841 gctcagtatt cccatcacct ctcgcggttt gagaatggga atacggccca tgatgcctaa
      901 acggtaaccg tctgagttcc aatatactct gcctcgagat ttggctctcc atcctcaaga
      961 agcatggaaa cgacctcttt gaggttctct cgaagctcat caagcgattc tccctgggta
     1021 tgggcgccgg gaaatccggg aacgaatcct acaaaaagtc ctgtttcaga gcacctttca
     1081 accacagcag taaaggcatg cataggggaa gtttagtcgt taaaagaggc catggcaacc
     1141 ttcagtttgc caagccgatg gtcaatatca tgtcccgcgc acctgcagca gctcggcggc
     1201 gatgctgacg gcgatttctt ccggcgtgtc cttgcccagg ggcaggccga tgggacagcg
     1261 gaacgccttc agggaatcct cggccacgcc ggcttcgcgg agctcctttc gcaacgtggc
     1321 ggcctttgag gcgctgccga tgacgccgag gtagggaaac gttcgggttt tcaggatccg
     1381 ctccaaaatg ggcagatccg tgcggtggcc ctgcgtcatg atcacgacaa acgccccgtc
     1441 cgggatttcc gaaacgatct cctccggcct ttccgcgagc tgggtttgga gattggcagc
     1501 ttggggaagt tgattgagcc actccggccg cgaatccacg acccgcaccc ggcattccag
     1561 cgtggcgaga aggcgcgcca gggcctgcga aacgtggccc gctccaaaca caaaaatccg
     1621 ccatgcgctc ggacggaagc tttcaaacag gaaggtcacc acgccgccgc aggtcatgcc
     1681 gatgtcgcgc tggaggttcc attccaccgt ttcgcagggg ctgggtggcg cggtttccag
     1741 gagcccgcgg gcatggcgga tgacggcttc ctcgacgcgc ccgccgccga tcgtgccgcc
     1801 cgcccggccc gcggcggtca cgagcatctt cgcgccgacc tcctgcggca cgctgccccg
     1861 ggcggtgatg agcgtcacgc cgacgaaggc ttcgcccgcc tgatcgagcg cggtcatttg
     1921 gggcaaaagc gcggtgaggg aatgcatcgg gcgtagcgaa tcaaattttg ggagcggcgg
     1981 cgagcggttc ccgaaaatag gcgagcgcct tttgataagc ctccggcgtg ttcacgtccc
     2041 agtccagcga gccgggcggc tctccgggca ttcgcatgag ctcggagtcg tggcggtcca
     2101 ggaagccgcg caatccccgg gagtcttcgt cgagggcccg cagctccggc aataacgagg
     2161 cggcgagcac gaccggatgc ccgcggcggc cggcccaatc gggaacgacg agggtctggc
     2221 cgccgggggg cgggaccgcg cggcgcaggc gaaggtaaag ccccggcagc cgcggttgat
     2281 ccaccgaggt gaggagaatc cactccgcgc gggcgggcag cctgctaatc ccggcggcga
     2341 gcgaggtggt cttgccagct tccggatggg ggttgagcgc cacgcgttca ccgcccagct
     2401 cccggctcca ggagtcaaaa tactcgggtc cggtcacgac gagcggctgc cagccattgg
     2461 cggcgaggga ttccatcatc cacggcagca gccgctgtcc gcccagccag gggaggct
//
LOCUS       DATDAY010000049         2068 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_2020370, whole genome shotgun sequence.
ACCESSION   DATDAY010000049 DATDAY010000000
VERSION     DATDAY010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2068)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2068
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2020370"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..202)
                     /locus_tag="VIS74_01015"
     CDS             complement(<1..202)
                     /locus_tag="VIS74_01015"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY5791846.1"
                     /translation="MSDDPSSLSARRGSVLLEQGRYPDAEKFFREALAGDPNDAYVLY
                     QLAVCELNQDRDAAALETIGRAT"
     gene            complement(199..1560)
                     /locus_tag="VIS74_01020"
     CDS             complement(199..1560)
                     /locus_tag="VIS74_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY5791847.1"
                     /translation="MSQLDALRQAIRVSPDNLPLLLLFAEACLAELSPDEGVAAFRQV
                     LEKEPGNAQALLGLAKGLHQSGKTSEAIVRLESLVRTHPNHAAALLQLSRFLMAEGQR
                     EPARECYEKSLKLPGGCRDTALEQELFLQGPPPAAPEKGRVTSEGWEAPPAAAAGTPE
                     ADGPHSPERPAVAFADVGGMEAVKEEIRMKIIYPLKNPDLFKAYGKKAGGGVLLYGPP
                     GCGKTLISRATAGEIEANFLSIGIHQILDLYIGESEKNLHRLFEMARDHAPSILFFDE
                     VDALAADRRDLRKSAGRTLINQFLAEMDGNVASNDGVLILGATNAPWHIDPAFRRPGR
                     FDRVLFVPPPDEAARAAILSVMARGKPVADLNARELAQRTKDFSGADLKAIFDAAVER
                     GLARAMQSGQIVPITQKELVETAKGLKPTTKAWFESAKNYALYSNQGGFYNDVLAFLG
                     IKK"
     gene            complement(1563..>2068)
                     /locus_tag="VIS74_01025"
     CDS             complement(1563..>2068)
                     /locus_tag="VIS74_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEY5791848.1"
                     /translation="DLFTIRELRGSLDDLKICFVGDGDCNMPRSWIWAAARLGFELRI
                     AAPEKFQPPKELVERAGGKVIVTGDVLAAAEGADVLYTDVWVSMGREEEAAYRLAQLQ
                     GYQINEETLAAAKPGVRVMHCLPAYRGKEITEEVLEAHASTIFQQAENRLHAQKAVLS
                     LIAQPLA"
BASE COUNT          345 a          699 c          650 g          374 t
ORIGIN      
        1 cggtggcccg cccgatggtc tccagcgccg ccgcgtcgcg gtcctggttc agctcgcaga
       61 ccgcgagttg atacaggaca tacgcgtcgt tcggatcgcc ggcgagcgcc tcgcggaaga
      121 atttctccgc gtccgggtaa cgcccctgct ccagcaggac cgaaccgcgc cgcgcgctca
      181 ggctggaggg atcgtcactc attttttgat gccgagaaag gccaggacgt cgttgtaaaa
      241 cccgccctgg ttggaataca gcgcgtaatt tttcgcgctc tcaaaccacg ccttcgtggt
      301 gggcttgagc cccttcgccg tctcgaccaa ttccttctgc gtgatcggaa cgatctggcc
      361 gctctgcatc gcccgggcca gcccgcgctc gacggcggcg tcgaaaatgg ccttcagatc
      421 ggcgccggaa aaatccttcg tccgctgcgc cagctcgcgc gcattcaaat ccgcgaccgg
      481 cttgccccgg gccatgaccg aaagaatcgc ggcgcgcgcc gcctcgtcgg gcggcggcac
      541 gaacagcacg cgatcgaacc gcccggggcg gcggaaggcc gggtcaatgt gccacggcgc
      601 attcgtcgcc ccgagaatga gcacgccgtc gttgcttgcg acattgccat ccatctcggc
      661 gaggaattga ttgatcaagg tccgccccgc gctcttgcgc aaatcccggc ggtcggcggc
      721 cagcgcgtcc acctcgtcga aaaacaggat gctcggcgcg tgatcgcggg ccatttcgaa
      781 gaggcggtgc agattttttt cgctctcgcc gatgtagaga tcgagaatct ggtggatgcc
      841 gatggataaa aaattcgcct cgatctcgcc cgccgtcgcg cggctgatga gcgtcttccc
      901 gcagccgggc gggccgtaaa gcagcacgcc gccgccggcc tttttgccgt aggccttgaa
      961 gagatccgga ttcttgagcg gataaatgat cttcatccgg atttcctcct tcaccgcctc
     1021 catgccgccg acatcggcga aggcaacggc ggggcgctcc ggggaatgcg ggccgtccgc
     1081 ttccggcgtt ccggccgccg cggcgggggg cgcctcccat ccctcgcttg tcacccggcc
     1141 cttttccggg gcggccggcg gaggcccctg caaaaacaat tcctgctcca gcgcggtgtc
     1201 gcggcatccg ccggggagct tgagggattt ttcgtaacac tcgcgcgccg gctcgcgctg
     1261 gccttcagcc atcaaaaacc ggctgagttg caacagcgcc gccgcgtggt tgggatgcgt
     1321 ccgcaccagc gattccagcc gcacgatggc ctcggaggtt ttgccggatt gatggagccc
     1381 cttcgcgaga ccgagcagcg cctgggcatt gccgggctcc ttttcgagca cctggcggaa
     1441 cgccgccacc ccttcgtccg gcgaaagttc ggccaggcag gcttcggcaa agagcagcaa
     1501 cagcggcaaa ttatccgggg agacgcgaat cgcttggcgg agggcatcca gctgggacat
     1561 ggtcaggcaa gcggttgggc gatcaggctc agcacggcct tctgcgcatg caggcgattt
     1621 tccgcctgct ggaaaatggt ggacgcgtgc gcctcgagca cttcctcggt gatttccttg
     1681 ccgcgatacg ccggcaggca atgcatgacc cgcacgccgg gtttggccgc ggccagcgtc
     1741 tcttcattga tctgataacc ctgaagctgg gcgaggcggt aggcggcttc ctcctcgcgg
     1801 cccatgctca cccacacatc ggtgtaaagc acgtccgcgc cctccgccgc ggcaagcacg
     1861 tcgccggtca cgatcacctt tccgcccgcg cgttcgacga gctccttcgg cggctggaat
     1921 ttctccggcg cggcgatgcg cagctcaaat ccgagccggg ccgccgccca gatccacgag
     1981 cgcggcatgt tgcagtcccc gtcgccgaca aagcaaattt tcaggtcgtc cagcgaaccg
     2041 cgcagttccc ggatggtaaa aaggtccg
//
LOCUS       DATDAY010000050         2740 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_1659208, whole genome shotgun sequence.
ACCESSION   DATDAY010000050 DATDAY010000000
VERSION     DATDAY010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2740)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2740)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2740
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1659208"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..443
                     /locus_tag="VIS74_01030"
     CDS             <1..443
                     /locus_tag="VIS74_01030"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HEY5791849.1"
                     /translation="PGESLAPLQHSASKDGSALANVTLVLLRHLKWPRAEAIEAVQTR
                     ARYMVSRLEKGLIVLEITTGVAPLLGLLGTLSGLVNIFANIGASGDPTLVARGISEAL
                     NTTIMGLAVAAPSLVAHNYFIRKVEIMSVEMEALLADLIQKTYR"
     gene            451..867
                     /locus_tag="VIS74_01035"
     CDS             451..867
                     /locus_tag="VIS74_01035"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEY5791850.1"
                     /translation="MKFYTKKQRAPIITIIPLIDILCILLIFFIVTTTFKKENNQLKI
                     NLPESKEAKQAAAEAKPPVLLRVQDADHLTLDDQPVTVAGLAAALKAQMGKEPGRGIA
                     LQADKEAPFGLIVKVLDALQAAGVKNIPAFTEPQKP"
     gene            864..1409
                     /gene="def"
                     /locus_tag="VIS74_01040"
     CDS             864..1409
                     /gene="def"
                     /locus_tag="VIS74_01040"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006983835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEY5791851.1"
                     /translation="MILEIVQYGHPALRSKGRKVAKIDDDLAKLIDDMLETMYEADGV
                     GLAAQQIGRPLQLCVIDVEGVAKRPSRMWVDGREVELEDYMPLVLVNPEVEPLGNPET
                     GTEGCLSFPGISADITRPERVRVKTQLFDGEALTFEAEGLLARAVQHEHDHLQGVLFI
                     DRMTPEDRKALGAKIEKIRTH"
     gene            1441..>2740
                     /locus_tag="VIS74_01045"
     CDS             1441..>2740
                     /locus_tag="VIS74_01045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5791852.1"
                     /translation="MLFWTVTAVIFAEWAVLYLPALRTMPGWYGDETLTLDIAENLIH
                     GRFAAAALFNTFATYIYQPIYLFFLGLGWQLSGGDIVGARFFNTVLALMIGLTLWVAG
                     RRVVGNVAAATAAIAFLGYEQTVIHFRQCFAHNGVAFGLTLSILLVCRKPSLGIDLKS
                     GLGLLFAVGSHPLGVYPAMVSGFTRLFSPRSWLPLIAPSALVLGLFYFCVYLRFGSWL
                     ISDIAIVGIEHEKIQAANQHHFWENLYRFYAQDSFHIIGLTGVLALLISCRWKQRIPL
                     AGIFLLLSLTLFSNRSNLTVFYYQAVVLLPLLSLGIGWMLVKIGALLARLIGKHFGTQ
                     SARIAKYLVPILAVTSFGISSAILVFSGKLLPRNQPWVTQSPHEVEEAAAWVNERTSS
                     EDVILANSNIGWLLKGRKANLLQYTAWTGLPTFMHEHPVPH"
BASE COUNT          565 a          795 c          759 g          621 t
ORIGIN      
        1 agccgggcga aagcctggct ccgctccagc attccgccag caaggatggt tccgctttgg
       61 cgaacgtcac gctcgtgctg ctccgccatt tgaaatggcc gcgcgccgaa gcgatcgaag
      121 ccgtgcaaac ccgcgcgcgc tacatggttt cgcggctgga aaaaggcctc atcgttctcg
      181 aaatcaccac gggcgtcgcc cccctgcttg gcctgctcgg caccctctcg gggctggtga
      241 acatcttcgc caacatcggc gcctcgggcg accccaccct ggtcgcccgc ggcatttccg
      301 aggcgttgaa taccaccatc atgggcctgg ccgtggcggc gcccagcctg gtcgcgcata
      361 attatttcat ccgcaaagtg gaaatcatgt ccgtggaaat ggaagcgctg ctggccgatc
      421 tgatccagaa gacttaccgc taggaagccc gtgaaattct acaccaagaa gcaaagagcg
      481 ccgatcatca cgatcattcc cctgatcgac attctctgca tcctgctgat ttttttcatc
      541 gtcacgacga cgttcaaaaa ggagaacaat cagctgaaga tcaatctgcc ggaatccaag
      601 gaagccaaac aggccgccgc cgaagccaaa cccccggtcc tgctgcgggt gcaggacgcg
      661 gaccatctca cgctcgacga ccagcccgtc accgtggccg gtctcgccgc cgccctcaag
      721 gcgcagatgg gaaaagagcc cggccgcggc atcgcgctcc aggcggacaa ggaagcgcct
      781 tttggtttga tcgttaaagt cctcgacgcg ctccaggccg ccggggttaa aaatatcccc
      841 gcctttaccg aaccccaaaa accatgattc tcgaaattgt ccaatacggc caccccgccc
      901 tgcgatccaa gggccgcaag gtggccaaga tcgacgacga tctggccaag ctgattgatg
      961 acatgcttga aaccatgtat gaagccgacg gcgtgggcct cgccgcgcag cagatcggcc
     1021 ggcccctgca actctgcgtc atcgacgtgg aaggggtggc caagcgcccg agccgcatgt
     1081 gggtggacgg ccgggaggtc gaattggagg actacatgcc gctcgtgctg gtgaatcccg
     1141 aagtcgagcc gctcggcaac ccggagaccg gaaccgaggg ctgcctgagt ttccccggaa
     1201 tctccgccga catcacccgc cccgagcggg tgcgcgtcaa gacccagttg ttcgatggag
     1261 aagccctcac cttcgaagcc gaaggcctgc tggcccgcgc cgtgcagcac gaacatgacc
     1321 acctgcaagg cgtgctgttc atcgaccgga tgacgccgga ggaccggaag gcgctgggcg
     1381 caaagatcga aaaaattcgg acacattgag aagcaaataa gaatgcgtct gcctcgccct
     1441 atgctcttct ggacggtgac tgccgtcata tttgccgaat gggcggttct ctatttgcca
     1501 gcgttgagga caatgcctgg ctggtatggg gatgaaaccc ttactttgga tattgcggag
     1561 aacctcattc acgggaggtt tgccgcagcc gcgctattta acaccttcgc cacttatatt
     1621 tatcagccga tttacctttt ctttctgggg ttaggctggc agctatcggg gggagatatt
     1681 gttggcgccc gtttttttaa tactgtcctt gccttgatga tcggcctgac cctgtgggtt
     1741 gccggaagac gcgttgtcgg aaacgtcgca gccgcgactg cggcgatagc gttccttgga
     1801 tatgaacaaa cggtgattca tttccggcag tgctttgcac ataatggagt cgcgtttggt
     1861 ctcacattgt cgattttgct ggtttgccgc aaaccgtcat tgggcattga tctgaaatcg
     1921 ggattgggac tgctgtttgc tgtgggttcg catccgctgg gggtttatcc ggcaatggtg
     1981 agtggtttca ccaggctgtt cagcccgcgg agctggctgc cgttgattgc gccttcggcc
     2041 ttggttctcg gattgttcta tttctgcgtt tacctgcgat tcggcagctg gcttatcagc
     2101 gacattgcaa ttgtcggcat tgagcacgaa aaaattcagg ccgcaaatca acatcatttt
     2161 tgggagaatc tctaccgctt ctatgctcaa gattcatttc atatcatcgg cttaaccgga
     2221 gtgctggcgc tgctgataag ctgccgatgg aaacagcgga taccgctggc gggcattttt
     2281 cttctgcttt cgttgaccct tttttccaat cgttcaaacc ttacggtatt ttactatcaa
     2341 gccgttgtct tgctgccgct gctctcattg ggcatcggat ggatgctggt aaaaatcggc
     2401 gcgcttttgg cccggttaat tgggaagcac ttcggcaccc agtccgcgcg gattgccaaa
     2461 tacttggtgc cgattttggc cgtcacttct tttggcatta gcagcgccat tctggtcttt
     2521 tcgggaaaac tgcttcccag aaatcaacct tgggtcaccc agtctccgca cgaggtcgag
     2581 gaggcggccg cctgggtaaa cgagagaacc tcgtccgaag atgtcattct tgcaaactcc
     2641 aacattggct ggctgctcaa aggaaggaaa gcaaaccttc tacaatatac tgcatggaca
     2701 ggtcttccca cctttatgca tgagcacccc gttccacatg
//
LOCUS       DATDAY010000051         5357 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_866339, whole genome shotgun sequence.
ACCESSION   DATDAY010000051 DATDAY010000000
VERSION     DATDAY010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 5357)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5357)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5357
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_866339"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            81..620
                     /locus_tag="VIS74_01050"
     CDS             81..620
                     /locus_tag="VIS74_01050"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HEY5791853.1"
                     /translation="MNEAKSLNVLGALSLAIADEMKLAASQAAEAGPAAPAALALLLH
                     SPGRTIDWLSGALDLSHSATVRLADRLEAEGWLARGAAGHDGRAVALTLTADGKKRAR
                     AVLNERRKVLTPLLRALTGEEKRVFAELLDKLIVVFARETGCNLDHVCRLCDESDCPE
                     RVCPLSCAIQARAGLTGAG"
     gene            complement(1201..2328)
                     /locus_tag="VIS74_01055"
     CDS             complement(1201..2328)
                     /locus_tag="VIS74_01055"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEY5791854.1"
                     /translation="MIVTHEGFLTIFELGNSWWVYAGSASLEGSESNDTLQANATVHH
                     LSGLAGNDSLTGTDSVNFLDGGTGRDTLTGGPGDDIYLVDNPGDVVMERADEGFDSVQ
                     ASLSYALGANLERLVFAGSRPLSGTGNALNNSIGGNDAANVLDGGAGNDFLSGMGGND
                     TIVGGAGGDVLAGGMGVDSLIGGLGNDTYSVDRGIDKVRERAGEGVDLVKASLSWTLD
                     ANVENLALTGAARSKGVGNTLDNILLGNAARNALFGGNGDDTLLGTAGNGRGETDWLA
                     GGSGDDLFVLGVKGHRLYDNLNAGASGRGDYAVIADFKPGHDRLQLAGGASQYYLGAP
                     HGENRGLFFETGAADELIAILQNPNPKALTDANVIGTAVFV"
     gene            2776..3876
                     /locus_tag="VIS74_01060"
     CDS             2776..3876
                     /locus_tag="VIS74_01060"
                     /inference="COORDINATES: protein motif:HMM:NF012290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEY5791855.1"
                     /translation="MRQLGVWIGVWLWAGAVMASAQVSRPSDPNSRELAVGVHDKPPY
                     AFYDGQEWRGLGVDLWRMIAGKLGYACHFQALPYEDLTRALEQKEVDVVVGELVVSAA
                     REEVIDFSQPFLQTNILVAVNREHWRPNWLKILLNAWDWNLLRVILGVAGALLIVTVG
                     LWLIERRAEESHFSGRSHYGLGSALWFTVVTMSGVGYGDKIPVTFLGRLVTIIWIFCG
                     LLLVTAYTATVASTVATAQTRSEVTQISNLKRQLNAVLAGSDAQHLLEEEGTRPEKFE
                     TLEAALTALADNKVNTVVGDGVSLRYLIQQNYAGKIELLPVHLSLAHVAIAIPQNSSL
                     RESINIALLEILPTPQWQKLVTQYVGDTPQNL"
     gene            3848..>5357
                     /locus_tag="VIS74_01065"
     CDS             3848..>5357
                     /locus_tag="VIS74_01065"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEY5791856.1"
                     /translation="MSETPRKTSSEPRAKVPTHQHEKWRLRYGHPLPFGASRVPGGVN
                     FAIYSKHATACTLVLFERGSEKPFVEIPFAREFRMGDVYAMTVFDLDFETIEYGYRFE
                     GPNEPAEGHLFDPKKIVLDPFAREVSGRDAWEKNPATSAVYPHRARIHSNNFEWGHDR
                     PLELPSNDLVIYEMHVRGFTRHPSSGVKCPGTYEGMREKIAYLRDLGVNCVELMPIFE
                     FDEWENPRSDPDTGEALTNYWGYSTIGFFAPKAGYSAAGIEGGQINELKALITELHAA
                     GIEIMLDVVFNHTAEGGDGGPVISFKGIDNKTYYLLNPDKSYANYTGCGNTLNCNHPI
                     VRHFVMDCMRYWAAEFHIDGFRFDLASVLSRDSHGVPMANPPLLESIAYDPVLAHCEL
                     VAEAWDAAGLYQVGNFPAYSRWNEWNGRYRDAARKFIKGDLGMAGEMVQRLLGSPDLY
                     APSGRKPQASINFITCHDGFTLRDLYSYSEKHNLANGEENRDGANDNYSWNCG"
BASE COUNT         1014 a         1778 c         1582 g          983 t
ORIGIN      
        1 actatcaatc cgcgcccatc cgtgatccgc ggtttaattc cggcttgacc tgacccaata
       61 aacatgcatg atgcatacat atgaacgaag ccaaatctct caatgtgctg ggcgccttgt
      121 cgctggcgat cgccgatgaa atgaagctgg ccgcttccca ggccgcggaa gcgggcccgg
      181 cggcgccggc ggccctcgcg ctgctcctgc attccccggg ccggaccatc gactggctca
      241 gcggggcgct ggatctctcc cattccgcca cggtgcggct cgccgaccgc ctggaggcgg
      301 aaggctggct cgcgcggggg gcggccggtc acgacggccg cgcggtggcc ctgacgctga
      361 ccgccgatgg gaaaaagcgc gcccgcgccg tgctcaatga acgccgcaag gtgctcaccc
      421 cgctcctccg cgccctcacc ggcgaggaaa agcgggtttt cgcggaactg ctcgacaagc
      481 tgattgtcgt tttcgcccgc gagaccggct gcaatctcga ccatgtttgc cgcctgtgcg
      541 acgaatcgga ctgcccggag cgggtctgcc cgctcagctg cgccatccag gcccgcgccg
      601 ggctcaccgg ggcgggttag cgcattttca gtttaattgg acgcgttcct ctcggcggcg
      661 ttggcaacgc cgctccaccg aaagatccgt ctggcggcaa aataaatgga aaacgcgcta
      721 agccgttccc aaaatcaatt ccggaaacct ggaaggaaac gcgttggacg cttcgctctg
      781 cccgcggccg cctgcagggc ggccgaagat cagcaatgcg gcgccctccc tttcggaaac
      841 gaatttggca accgtttcaa gccggcttca aagggagcaa cggacggctc ggcgagccat
      901 ccctgccttt taaaggggca gggcgggatc gccgatcccg ccgtggctta gggaaacgcg
      961 gatcaaatcg gatgaaggga aagatccgcc gatttcaccg attttcgcag attttttgcg
     1021 gcgaattctg cggatgaaat ccgcgtttcc ttagcagcgc attcccccgg cggggttggc
     1081 aaccccgccc taccctcacc accaactaaa ccggcctccc ggtggggcgg cgttgccaac
     1141 gccgccgctc actcggcaaa ctgaatccgt taaggaaccg ctgatcaaat ccgcgccacc
     1201 ctaaacaaaa accgccgtgc cgatcacgtt ggcgtccgtg agcgccttcg ggttcgggtt
     1261 ttgcaggatg gcgataagct cgtccgccgc gcccgtctcg aagaacaggc cccggttctc
     1321 cccgtgcggc gcgccgagat aatactgtga cgcgccgccc gcgagctgga gccggtcgtg
     1381 ccccggcttg aaatccgcga ttaccgcgta gtcgccgcgc ccggaggcgc cggcattcaa
     1441 attatcgtaa aggcggtggc ccttcacgcc gagcacgaag agatcgtcgc cagagccgcc
     1501 ggccagccag tcggtctcgc cgcgcccgtt tcccgccgtt cccaggagcg tgtcgtcgcc
     1561 gttgccgcca aagagcgcgt tccgcgcggc attgccgagc aggatgttgt ccagcgtgtt
     1621 gccgacgcct ttcgagcgcg cggcgcccgt gagggccaaa ttttcgacgt tggcgtcgag
     1681 cgtccagctc agactcgctt tgacaaggtc aacgccttct cccgcccgct cccgcacctt
     1741 gtcgattccg cggtcaacgg agtaagtgtc attccccagg ccgccaatca ggctgtccac
     1801 tcccatgcct ccggcgagga cgtcgccgcc cgcgccgccg acgatcgtat cgttcccgcc
     1861 catgccggac aggaaatcgt tgcccgctcc tccgtccagg acattggcgg cgtcattgcc
     1921 gccgatggag ttattcaagg cgttccccgt gccggagagc ggccgggagc cggcaaaaac
     1981 gagacgttcc aggttggcgc ccaaggcgta gctgagggac gcctgcacgg agtcgaatcc
     2041 ctcgtccgcg cgctccatca cgacatcccc cgggttatcg acgagataga tatcatcgcc
     2101 cggacctccg gtgagcgtat ccctgcccgt tcccccatcg aggaaattga cggagtccgt
     2161 tccggtgagc gaatcattcc ccgccaatcc ggagagatgg tggacggtgg cgtttgcctg
     2221 gagcgtgtcg ttggattcgc tgccttccag agaagcggag cctgcataaa cccaccagct
     2281 gttcccaagc tcgaaaattg tcaagaagcc ctcatgggta acgatcactc ccccggtgat
     2341 ctcatttctg atgaggctct cgaaagaact cccgccaaca ctggtggtga tgacacccgc
     2401 gtaagtggaa cccgcgaagg taagcgttcc gctgcctgtc ttagtgaggc cttttccggc
     2461 ggcggcgggt atcatttttc ggaggagatt gattgggtgt taaccgagac ggaaatttct
     2521 ttcaatgtct tccgccggcc aaaagtcagc gcggcgcagc ggagcggcgg gtttttgatt
     2581 tcaaaacgac ccgtccaagt tttgaaccgc cctcgcgccg cccggaaccg cgttttgcaa
     2641 gaaagaagcc gttcgccaag gagtggaaag ggaacccgtg ggcgcttgct tctcccgcta
     2701 ttcccaagcg ccgctccgga ttcccgtgat ttaccgcttt taatcaccgc gcggcttggg
     2761 caatgtctgt ttccaatgag gcaactcggc gtttggatcg gagtctggct gtgggcgggc
     2821 gcagtgatgg caagcgccca ggtcagccga ccttccgacc cgaactcgcg cgaactcgcc
     2881 gtcggcgttc acgacaagcc gccctacgca ttttacgatg gccaggaatg gcgcgggctg
     2941 ggcgtggacc tctggcggat gatcgccggc aagctcgggt atgcctgcca tttccaggcc
     3001 ctgccgtatg aggatctcac ccgggcgctg gagcaaaagg aggtggatgt ggtggtgggc
     3061 gaattggtcg tcagcgccgc gagggaggag gtcatcgatt tttcccagcc ctttttgcag
     3121 acgaacatcc tcgtcgccgt caatcgcgag cactggcggc cgaattggct gaagatcctg
     3181 ctcaacgcct gggactggaa cctgttgcgc gtgatcctcg gcgtcgcggg ggcgctcttg
     3241 attgtgaccg tgggcctctg gctgatcgaa cgccgggcgg aggaaagcca tttttccggc
     3301 cggtcccatt acgggctggg ctccgcgctt tggttcaccg tggtcaccat gtcgggggtc
     3361 ggctacgggg acaagattcc ggtcacgttt ctcggccggc tggtcaccat catctggatt
     3421 ttctgcggcc tgctgctggt gacggcttac acggccacgg tcgcctccac ggtcgccacc
     3481 gcgcagacgc gctcggaggt gacccagatc tccaacctca aacgccagct taacgcggtg
     3541 ctggcgggca gcgacgccca gcacctgctg gaggaggagg gaacccgccc agaaaaattt
     3601 gaaaccctgg aagccgcgct caccgcgctg gcggacaaca aggtgaatac cgtcgtcggc
     3661 gacggcgttt cgctgcgtta cctgatccag cagaattatg cgggcaagat cgaactgctc
     3721 cccgtccacc tgagcctggc ccatgtcgca atcgccattc cccaaaacag ctccctccgt
     3781 gaatccatca acatcgccct gctggaaatc cttcccactc cgcaatggca gaaactcgta
     3841 acgcaatatg tcggagacac cccgcaaaac ctctagcgaa ccccgggcca aggtgcccac
     3901 gcatcaacac gaaaaatggc ggctgcgcta cggccatccg ctgcccttcg gcgccagccg
     3961 cgtgccgggc ggggtgaact tcgcgattta ttccaagcac gccacggcct gcacgctggt
     4021 gcttttcgaa cgcggctcgg aaaagccgtt cgtcgagatt cccttcgcgc gcgaattccg
     4081 catgggggat gtgtatgcga tgaccgtctt cgacctcgat tttgaaacga tcgaatacgg
     4141 ctaccgcttc gagggcccga acgagccggc cgaggggcat ctgttcgatc cgaaaaaaat
     4201 cgtcctcgat ccctttgccc gcgaggtttc cgggcgggac gcctgggaaa aaaatcccgc
     4261 cacctccgct gtctatccgc accgcgcgcg catccacagc aacaacttcg aatggggcca
     4321 cgaccggccg ctcgagctgc cgtcgaacga tctcgtcatc tacgaaatgc acgtccgcgg
     4381 ctttacgcgg catcccagct cgggcgtcaa atgccccggc acctacgaag gcatgcgcga
     4441 gaaaatcgcc tacctccggg atctcggcgt gaactgcgtg gagctcatgc ccatcttcga
     4501 attcgacgaa tgggaaaatc cccgcagcga tcccgacacg ggcgaggcgc tcaccaatta
     4561 ctggggctac agcactatcg gcttcttcgc gccgaaggcc ggttactccg ccgccggcat
     4621 tgagggcggg cagatcaacg agctcaaggc gctcatcacc gaactccacg ccgccggcat
     4681 cgagatcatg ctcgacgtcg tgttcaacca caccgcggaa ggcggcgacg gcgggccggt
     4741 tatttcgttc aagggcatcg acaacaaaac ctactacctc ctcaaccccg acaagtccta
     4801 cgcgaactac accggctgcg gaaacacgct gaactgcaac cacccgatcg tccgccattt
     4861 cgtgatggat tgcatgcgct attgggcggc ggaatttcac atcgacggct tccgcttcga
     4921 cctcgcctcc gtgctcagcc gggacagcca cggcgtgccc atggcgaacc cgccgctgct
     4981 cgaatccatc gcctatgatc ccgtgctcgc gcactgcgaa ctcgtcgccg aggcgtggga
     5041 tgccgccggc ctttaccagg tcggcaactt ccccgcctac agccgctgga acgaatggaa
     5101 cggccgctac cgcgacgccg cccgcaaatt catcaaggga gacctcggca tggccggcga
     5161 aatggtgcag cgcctcctcg gctcgcccga tctctacgcg cccagcggcc gcaagccgca
     5221 ggcgtccatt aatttcatca cctgccacga cggcttcacg ctgcgcgacc tctattccta
     5281 cagcgagaaa cacaacctcg ccaacggcga ggaaaaccgc gatggcgcga acgacaacta
     5341 cagctggaat tgcggcg
//
LOCUS       DATDAY010000052         2598 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_1484703, whole genome shotgun sequence.
ACCESSION   DATDAY010000052 DATDAY010000000
VERSION     DATDAY010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2598)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2598)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2598
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1484703"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..426
                     /locus_tag="VIS74_01070"
     CDS             <1..426
                     /locus_tag="VIS74_01070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5791857.1"
                     /translation="NQMDVGKIKVGDYLQVKMQGAAERPLKARVVFIAPVATVKNNIK
                     GFAVESVIEELDDRLRPGMSVSMELSVARAKDTLAVPIGAVFTEDEHKYVYVRRGEST
                     ERRQVEIGLTNFSFAEVKSGLNEGEEVLLVAPRNAPKKS"
     gene            430..1119
                     /locus_tag="VIS74_01075"
     CDS             430..1119
                     /locus_tag="VIS74_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY5791858.1"
                     /translation="MPNRVLRLEEVSRIYRIGGQEIRALDRLSLEIFEGEFTAIIGPS
                     GSGKSTLMHLIGGLDTPQEGRLEICGHDLTQASADELAEVRNREIGFVFQSFNLLPRF
                     NVVENIELPMIYRGIGPSQRRRRAEALARQVGLEERLGNRPAQLSGGQCQRVAIARAL
                     ANEPRILLGDEPTGNLDSRTGEMILDLFRELHRSGRTVVLVTHDQEIADVCERVVEIK
                     DGKIRREYRNP"
     gene            1193..2485
                     /locus_tag="VIS74_01080"
     CDS             1193..2485
                     /locus_tag="VIS74_01080"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY5791859.1"
                     /translation="MNLFAAFEVGLREILSHKFRSFLSMLGIVLGVSSLVATMAITAG
                     IERGTRKVMQQIGGLERVGVTKKDISSEELDFWTFSPGRTLQDAYAIKKSAPLISHIS
                     PEISEYAGIASEAANQGRGVGGVYPDYFPINQHELAAGRFITDIDVENGNRVVVLGQL
                     VCDKLFPGQTPWQIAGQFVYLNQIPFEVVGVLKNYQTEANRMTRPGGNRRGGWDPFRG
                     KNETTVIPFTTLFYELKSGKFPQDTPDSVPLQNLYLRVGDLNFFQEALDQVRTALEIT
                     HRGVEDFDFETRQDFFDRIESSMRSTRISGGLIAAISLLVGGIGITNIMLASISERVR
                     EIGIRLAIGARGRDIFVQIIVESVTIAILGGLFGVVVGVGLIQLLTWLAPGDNLPVLT
                     WMSVVLSVCFATLAGVLSGIYPAMKAAGLDPISALRYE"
BASE COUNT          505 a          772 c          839 g          482 t
ORIGIN      
        1 aaccagatgg acgtgggcaa gatcaaggtg ggcgactacc tgcaggtcaa aatgcagggc
       61 gcggcggaga ggccgctgaa ggcccgggtg gtgttcatcg cgccggtggc gacggtcaag
      121 aacaacatca agggttttgc cgtcgagtcg gtcattgaag aactcgacga ccggctccgc
      181 cccggaatgt cggtgagcat ggagctttcg gtggcccgcg ccaaagacac gctggccgtt
      241 cccatcggcg cggtgttcac ggaggatgag cacaaatacg tgtatgtgcg ccgcggcgaa
      301 tccaccgaac ggcggcaggt ggaaatcggg ctgacgaatt tttccttcgc ggaggtcaaa
      361 tccggtctca atgaagggga ggaagtgctg ctggtcgccc cccggaacgc gccgaagaag
      421 agttagctga tgcccaaccg cgttctccgg cttgaggaag tttcccggat ttaccggatc
      481 ggcggccagg aaatccgcgc gcttgaccgg ctgagcctgg aaattttcga aggcgagttt
      541 accgcgatca tcggccccag cggcagcggc aaatccacgc tcatgcatct catcggcggc
      601 ttggacacgc cgcaggaggg gcggctggaa atctgcgggc acgatctcac ccaggcctcg
      661 gcggacgaac tcgcggaggt ccggaaccgc gagatcggtt ttgtcttcca gtccttcaat
      721 ctgctgccgc gcttcaatgt ggtggaaaac atcgagctgc cgatgattta ccgggggatc
      781 ggccccagcc agcggcgccg gcgcgcggaa gcgctggccc ggcaagtcgg tttggaggaa
      841 cgcctgggca accgccccgc gcagttgagc gggggccagt gccagcgcgt ggccatcgcc
      901 cgggccctcg cgaatgaacc ccgcatcctt ctgggcgacg aacccaccgg caacctcgac
      961 agccgcacgg gcgaaatgat tctcgacctg ttccgcgagc tgcaccgcag cgggcggacg
     1021 gtggtgctcg tgacccacga ccaggaaatc gccgacgtct gcgagcgggt ggtcgaaatc
     1081 aaggacggca aaatccgccg ggagtatcgg aacccatgaa aaggggggaa ttgcggaatg
     1141 gcgagacgaa gccaatccat ccgcaatccg caatccgcat tccgaaatcg ccatgaattt
     1201 atttgccgca ttcgaggtgg ggttgcgcga gatcctttcg cacaaattcc gcagcttcct
     1261 ttccatgctc ggcattgtgc tgggagtgag cagcctcgtg gccaccatgg cgatcacggc
     1321 gggcatcgag cggggcaccc ggaaggtcat gcagcagatc ggcggcctcg agcgcgtggg
     1381 ggtgacgaag aaggacatca gctccgaaga gctggatttt tggaccttct ctccgggccg
     1441 caccttgcag gacgcttatg ccattaaaaa atcggcgccg ctgatcagcc acatctcgcc
     1501 ggaaatttcc gaatacgccg gcatcgccag cgaggccgcc aaccaggggc ggggcgtggg
     1561 cggcgtgtat ccggattatt ttcccatcaa ccagcatgaa ctggccgcgg ggcgcttcat
     1621 taccgatatc gacgtggaaa acggcaatcg ggtcgtggtg ctgggccaat tggtttgcga
     1681 caagcttttc cccggccaga cgccctggca gatcgccggc cagttcgttt atctgaatca
     1741 gattcccttc gaggtcgtcg gggttttgaa aaattatcaa accgaagcca accggatgac
     1801 gcggcccggc ggcaatcgcc ggggcggatg ggatccgttt cgcggcaaga atgagaccac
     1861 cgtcattcct ttcacgacgc tgttttacga gctgaagtcc gggaaattcc cccaggacac
     1921 gccggattcc gtgccgctgc agaaccttta cctgcgcgtg ggcgacctga attttttcca
     1981 ggaggcgctg gaccaggttc ggaccgccct ggagatcacc catcgcgggg tggaggattt
     2041 tgactttgaa acgcgccagg acttttttga ccggatcgaa agcagcatgc ggtccacccg
     2101 catcagcggc gggttgatcg ccgccatcag cctgctggtc gggggcatcg gcatcaccaa
     2161 catcatgctc gccagcattt cggaacgggt ccgggagatt ggcattcgcc tcgccatcgg
     2221 cgcgcgcggc cgggacatct tcgttcagat catcgtggag agcgtgacga tcgccattct
     2281 cggcggcctg ttcggcgtgg tcgtcggcgt gggactcatt caattgctga cctggcttgc
     2341 ccccggcgac aatctgcccg tgctgacctg gatgagcgtg gtcctgagcg tctgtttcgc
     2401 gacgctggcc ggggtgctct ccggcatcta cccggccatg aaggccgccg gcctcgaccc
     2461 gatctccgcg ttgcgttacg agtgatcggc ctgtagccgg gatcgccgat cccggctgca
     2521 gggctctaaa aaagcagcgg aatgatcaga atcgccacga tgttgacgac cttgatcatg
     2581 ggattgatgg ccgggccg
//
LOCUS       DATDAY010000053         2792 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_891772, whole genome shotgun sequence.
ACCESSION   DATDAY010000053 DATDAY010000000
VERSION     DATDAY010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2792)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2792)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2792
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_891772"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..843)
                     /locus_tag="VIS74_01085"
     CDS             complement(<1..843)
                     /locus_tag="VIS74_01085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5791860.1"
                     /translation="MISKLKGVLAAVMGFGLCLGSGPAAEPRSLGDLVNHPEGATVPD
                     MLAVLDQEAGKSDSSFEFGRVFANACRRADGGTLPKFIRIFNAWSAPAYVKGAPFAVL
                     AEAWLRLRTPETLPTRLVFPDPPPEPRAAPEDYPEELRRAAECYLQVAEPFRKLRDAR
                     ISREKGVDFQTHESEYWRLIGQLLEGKDGPFAGQLLAYRWGGGCGTGSDAFEDPQSHA
                     LVLALAADGRWAEAAGAALSVAPSPETDGAIRVLSACVPDPRQVVIGGLAFLATRPDG
                     VWLER"
     gene            complement(840..2084)
                     /locus_tag="VIS74_01090"
     CDS             complement(840..2084)
                     /locus_tag="VIS74_01090"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HEY5791861.1"
                     /translation="MKDRPSPPLIDPALGRDLFDFLLANTPDQVYFKDKEGRFLRASH
                     AVASFLNAPNTGELIGKSDFDFWSPETAQAARADEVRIMETGQPLIGKIERLVHPDGR
                     IHWDHTTKLPLRDSRGEIIGICGINKDFTEIKLMQDALREERNRLRILTAELREKNTQ
                     LEDDLRMAREVQEALLPRESSAVAGAGLMGGLSFAHCYRPSAAVGGDFFYFVPISDRE
                     AGIFICDVMGHGLRAALITAIIRACLEELRPDMADPGRFLNRLNHRLRTILNRVDEPF
                     LATAFFMVVTPSAHEVRFANAGHPAPVCLRRTDGTAKILAEAVRQPVGALGLFAETNY
                     RTAYSPFERNDRIVLFTDGLYEVDSPQGGEFGIPSLMKSLGRHASLPAHDLFTAVLED
                     VSQFSHRHNFDDDVCLVSVEQA"
     gene            complement(2311..>2792)
                     /locus_tag="VIS74_01095"
     CDS             complement(2311..>2792)
                     /locus_tag="VIS74_01095"
                     /inference="COORDINATES: protein motif:HMM:NF014822.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha amylase C-terminal domain-containing
                     protein"
                     /protein_id="HEY5791862.1"
                     /translation="KQWTDWQGKPELLIWWEGLEGRDKAMSDQHRFTRDLMWLRRKHP
                     ALRGEGLNVFHVHNDNRVIAFHRWLPGIGRDVVVVASLNERTFYNHSYRIGFPGGGYW
                     REVFNSDVYDNWVNPHVQGNPRGITADGPAWDGHPVSAGITLPANSLLVFARDGGDS"
BASE COUNT          520 a          892 c          904 g          476 t
ORIGIN      
        1 gcgttcgagc cagactccat ccggccgggt ggccaggaaa gccagcccgc cgatcacgac
       61 ttgccggggg tcgggaacgc aggcggaaag cacgcgaatg gcgccgtcgg tttccgggga
      121 tggcgccacg cttaaagccg cgccggcggc ctccgcccag cgcccatccg cggccagcgc
      181 cagcaccagg gcgtgggact gcggatcttc aaaggcatcg ctgcccgtgc cgcaccctcc
      241 gccccagcgg taagccagca gctggccggc gaacggtcca tcttttccct ccagcaattg
      301 cccgatcagc cgccagtatt ccgattcgtg cgtctggaaa tccacgccct tctcgcggga
      361 gatgcgcgcg tcccgcagct tccgaaacgg ctccgccacc tgcaaataac attccgccgc
      421 ccggcgcagt tcttcgggat aatcctccgg cgccgcgcgc ggttccggcg gcgggtcggg
      481 aaaaaccagg cgggtgggaa gagtttccgg ggttcggagc cgcagccagg cctcggcaag
      541 cacggcaaag ggggctccct tcacgtaggc gggcgcggac caagcgttga aaatccggat
      601 gaatttcggc agggtcccgc cgtccgcccg gcggcaggca ttcgcaaaaa cccggccgaa
      661 ctcgaacgag gaatcgcttt ttcccgcctc ctgatccagg acggcgagca tgtccggaac
      721 ggttgcgcct tccggatggt tcacgagatc gccgagactt cgtggttcgg cggccggccc
      781 gctgcccagg caaaggccaa accccatcac cgcggcgagg acgcccttta gcttggaaat
      841 catgcctgct cgaccgacac caggcagacg tcgtcgtcga agttgtgccg atgggagaat
      901 tgcgaaacgt cctccagcac cgcggtaaaa aggtcgtggg ccggaaggct ggcgtggcgg
      961 cccaggctct tcattagcga gggaatgcca aattccccgc cctggggcga atccacctca
     1021 tacaggccgt cggtgaagag gacgatgcgg tcattccgct cgaatggcga atacgccgtg
     1081 cggtaattcg tctcggcgaa aagccccagc gcgcccaccg gctgccggac ggcctcagcg
     1141 agaatcttag ccgtgccatc cgtgcgccgc aggcacaccg gggcgggatg gcccgcgttg
     1201 gcgaaccgca cctcgtgggc cgagggcgtc acgaccataa aaaacgccgt ggccaggaag
     1261 ggttcgtcca cgcggttgag gatcgtccgc aggcggtggt tcaggcggtt gagaaaacgc
     1321 ccgggatcgg ccatgtcggg gcgcaattcc tccaggcagg cgcggatgat cgccgtgatc
     1381 agcgcggcgc ggaggccgtg ccccatcaca tcgcaaataa agatgcccgc ctcgcgatcc
     1441 gagatcggca cgaaataaaa gaaatccccg cccaccgccg cggagggacg gtagcagtgc
     1501 gcgaacgaca gcccgcccat gaggccggcg cccgcgaccg cggaggactc gcggggcagg
     1561 agcgcctcct ggacttcgcg ggccatgcgg agatcgtcct ccaactgggt gttcttttcc
     1621 cgcagttccg cggtcaggat gcggaggcgg ttgcgctcct cgcggagggc gtcctgcatg
     1681 agcttgatct cggtgaaatc cttgttgatg ccgcaaatgc cgatgatctc gccgcgcgag
     1741 tcccgcagcg gcagcttggt ggtgtggtcc cagtggatgc ggccatccgg atgcaccagc
     1801 cgttcgattt ttccaatgag cggctgcccg gtttccatga tgcgcacctc atcggcccgc
     1861 gcggcctgcg ccgtttccgg cgaccagaaa tcgaagtcgc ttttgccgat cagttcgccc
     1921 gtgtttgggg cattgagaaa gctggcgacg gcatggctgg cgcgcaagaa gcgcccctcc
     1981 ttgtccttga aataaacctg gtcgggggtg ttcgcgagca ggaagtcgaa gagatcccgg
     2041 cccagggccg gatcaatgag gggaggagag ggccggtctt tcacatcggg tattttttag
     2101 tgaggcgcct tgatcgccgc cggcggatgg ccagccgcag gcaaaaccat tccgatccag
     2161 gagcgagtgg gggtctccat gaccctacct gtatagccgg acattccggg aaagcaagaa
     2221 cgatggcgcc catcgattcg ggggaaaaca agatttttta aaccgcggag gccgtccatt
     2281 ccctcccggc cgggcggtca atgcggcctt ttaagagtcg ccgccgtccc gggcgaatac
     2341 caggaggcta tttgccggga gggtaatgcc tgcggaaacc ggatgtccgt cccacgccgg
     2401 gccatcggcc gtgatgccgc gcggatttcc ctgaacgtgg ggattgaccc agttgtcata
     2461 gacatcgctg ttgaagacct cccgccaata gccgccgccc ggaaagccga tccgataact
     2521 atggttgtag aaggttcttt cgttcaggct ggcaacgacg accacatcgc gcccgatgcc
     2581 gggcagccaa cggtggaagg cgatcactcg attgtcgttg tggacatgga agacattcag
     2641 cccttccccg cgcagggcgg gatgtttgcg gcgcagccac atgagatccc gggtaaagcg
     2701 atgctgatcg gacatcgcct tgtccctgcc ttcgagtccc tcccaccaga tcagaagctc
     2761 cggcttgccc tgccaatcgg tccattgctt gt
//
LOCUS       DATDAY010000054         2435 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_2619020, whole genome shotgun sequence.
ACCESSION   DATDAY010000054 DATDAY010000000
VERSION     DATDAY010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2435)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2435)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2435
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2619020"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1450)
                     /locus_tag="VIS74_01100"
     CDS             complement(<1..1450)
                     /locus_tag="VIS74_01100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5791863.1"
                     /translation="MKLSRCLPGFLLLPCLPLAAAELEDAARVIQQLQQSAESPANGE
                     DDSPSARWKKALADYSAQSPSLPPDEAARQWLVLAKQWEKLSPSASHSYHHSSDNSFN
                     SLIKVLPPPAVWEALQKQTAQAPLGDPLPDALLALLAATLVNDEAGKWKGLDSIRQIG
                     GSPDPGSAILQSITRLMGGSNASDWMKSSIGQLADAFVETSDDPAHILAALEIQLQLR
                     GKEKGASLTIPDLVTLAGEAKARAFLQKALALPLELNVRGIQTQALAREIAVRNPGSL
                     AMPQWGLVAGIDESNLQLYAALAGKFPDSGESDYERRTAAGYYLLGLIAKGRTADASR
                     FVLESKPGDLGLPYQGLKAMRAAGYGGAIYEFLHTLLEKNPDLPFWSDFLAVAAEERK
                     TAGALELAQRALQRPQLTDAQRQQVLQELAHAYLAHGETAKGVALLRERIQLLKAAPS
                     PDESEISRTAMEMVALGRLLPDSALREEGLKEA"
     gene            1726..2391
                     /gene="infC"
                     /locus_tag="VIS74_01105"
     CDS             1726..2391
                     /gene="infC"
                     /locus_tag="VIS74_01105"
                     /inference="COORDINATES: protein motif:HMM:TIGR00168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEY5791864.1"
                     /translation="MQLRTACGQPNPGGTINLKDIRVNERIRAREVRVIDGTNNQQIG
                     VMAIGDAVRRARSMGLDLVEVSPNAVPPVCRIVDYGKFRYDLSKQDKDRKHSSAGKIK
                     EVKFRVNIDQHDYETKLRHAEEFLDKGFKVKVHLQFRGREMAHQDLGMKVMIRVKNDL
                     IDMGQVDMDPKLVGKAISMTLSPLPASKRKRKFHLDEAEEAEAPEPSLSNRLPLPTDL
                     PGN"
BASE COUNT          468 a          745 c          767 g          455 t
ORIGIN      
        1 gggcttcctt caagccctcc tcccgcaacg cggaatccgg cagcagccgg ccgagcgcga
       61 ccatctccat ggcggtgcgg ctgatttcgg actcgtccgg ggagggagcc gcttttaata
      121 actgaatccg ttctcgcagc aaggccacgc ctttggcggt ttccccgtgg gcgaggtagg
      181 catgggcgag ctcctggagc acctgctggc gctgggcgtc ggtcaattgc ggacgctgca
      241 aggcgcgctg cgccagctcg agggccccgg cggttttccg ctcctcggcg gctacggcca
      301 ggaaatcgct ccagaacggc agatcgggat ttttttccag aagggtgtgc aggaactcat
      361 aaatcgcccc gccgtaaccc gccgcgcgca tggccttgag tccctgatac ggcagcccaa
      421 ggtcgccggg cttggactca agaacgaacc ggctggcgtc cgcggtccgg cctttggcga
      481 tgaggcccag gagataatac ccggccgcgg tgcggcgttc gtagtccgac tcgccgctgt
      541 ccgggaattt tccggccagc gcggcataga gttggaggtt ggattcgtcg atcccggcaa
      601 ccagccccca ttgcggcatc gcgagggagc cggggttccg gacggcgatt tcccgggcca
      661 gcgcctgggt ttggatgccc cggacattga gttccagcgg caaggccagc gccttttgca
      721 aaaaggcccg ggccttggct tccccggcga gggtgaccag atcgggaatg gtcagcgagg
      781 cgcctttctc cttgccccgg agctgaagct ggatctccaa cgccgcgagg atgtgcgcgg
      841 gatcgtcgga cgtttccacg aacgcatcgg cgagctggcc gatggagctt ttcatccagt
      901 cgctggcgtt cgatcccccc atcaaacgcg tgatggattg caaaatggcg gacccggggt
      961 cgggggagcc tccgatttgc cgaatggaat ccagcccctt ccatttgccc gcctcgtcgt
     1021 tgacgagcgt ggcggcaagc aaggccagca acgcatccgg caggggatcg cccagcggag
     1081 cctgggccgt ttgtttttgg agggcctccc aaacggccgg cggcggaagg actttgatca
     1141 gcgaattgaa cgagttatcc gacgagtgat ggtaagaatg cgacgcggac ggcgaaagtt
     1201 tctcccattg cttggccaga accagccact gccgggccgc ctcgtccggg ggcaggctgg
     1261 gggattgcgc ggagtaatcc gccagggctt ttttccatcg ggcggagggc gagtcgtcct
     1321 cgccgtttgc cgggctctcc gccgactgct ggagttgctg gatgacgcgg gcggcgtcct
     1381 ccaattccgc ggcggcaagc gggaggcagg ggaggagaag gaatccgggg aggcagcggg
     1441 agagcttcat ggggagagcc taaacaaatt ttgcggcaaa ccgcaccaaa tctctcgcaa
     1501 aattcccctt gcgccgattc gcaaagtggc ttagatgctc gattcagtgg aattcaaccg
     1561 gaacagccaa tttttatccg ccgcccccgg ggcaaggatg agtcgtattc cggtcacacc
     1621 gttttctgtc aatcctgccg tgacttctca gacattcctc gcgtgagcca ggtgattttc
     1681 cggacagtga ccttgatttt ttccgctcat ccatgagctg cccttttgca actccgcacc
     1741 gcgtgcggcc aacccaaccc aggaggcacc attaatctca aagacatccg cgtcaatgaa
     1801 cgcattcgcg cccgtgaggt gcgcgtgatt gacggaacca acaaccaaca aattggcgtc
     1861 atggccattg gcgacgccgt tcgccgggcc cgcagcatgg ggctcgatct cgtcgaagtc
     1921 tcgccgaacg cggtcccgcc ggtctgccgg atcgtggatt atggcaagtt ccgttacgat
     1981 ctctcgaagc aggacaagga ccgcaaacac agctccgccg gcaaaatcaa ggaagtcaaa
     2041 ttccgcgtga acattgacca gcacgactac gagacgaagc tgcgccacgc cgaggaattt
     2101 ctcgacaagg ggttcaaggt gaaggtccac ctgcagttcc gcggccgcga aatggcgcac
     2161 caggatctcg gcatgaaagt gatgatccgc gtcaagaacg acctcatcga catgggccag
     2221 gtggacatgg atcccaagct cgtcggcaag gcgatttcca tgaccctcag tccgctgccg
     2281 gccagcaagc gcaaaaggaa attccatctg gacgaagcgg aggaggcgga agctcccgaa
     2341 cccagcttga gcaaccgcct gccgctgcca accgaccttc cgggcaactg atttggcgga
     2401 tcacttttct tccgggcggc gcctgctgct gtttg
//
LOCUS       DATDAY010000055         3101 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_1337447, whole genome shotgun sequence.
ACCESSION   DATDAY010000055 DATDAY010000000
VERSION     DATDAY010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 3101)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3101)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3101
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1337447"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..331)
                     /locus_tag="VIS74_01110"
     CDS             complement(<1..331)
                     /locus_tag="VIS74_01110"
                     /inference="COORDINATES: protein motif:HMM:NF024882.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HEY5791865.1"
                     /translation="MNRFRKTNPWKEVIGIGLILLGVLLFVALISFDKGDLPGWAPLI
                     YSTSQQNEPLHNLVGVVGALLAGYSYFLLGAAAYWLALIVVGFGIGKLLVENFQLLIR
                     CGWGVVNL"
     gene            334..1134
                     /locus_tag="VIS74_01115"
     CDS             334..1134
                     /locus_tag="VIS74_01115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5791866.1"
                     /translation="METNFMHHRHSRDKGSVEIFWRKNAPPRAKMTFPNHAPNSGLTL
                     RPRSPKTPKSIMKILPLALFATLLGAASGFAGSFGPPPFSNGSPLQSGTDGTYQATLS
                     GTNMTGILSFAMQNGSQTGSGSQNQWVAFQHGAVFRGNVQANMVKTQITGILDSATLE
                     LPEDDDGRIELPVVFLIQGNRASGAFTANIDFEDPYGKFTGQGHIEGSTSDSITIFGI
                     FTNVFGDQGTITFSTNLPSGTVQRAAFRLRGTRTSTSASGTAGSSTNG"
     gene            1189..1707
                     /locus_tag="VIS74_01120"
     CDS             1189..1707
                     /locus_tag="VIS74_01120"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEY5791867.1"
                     /translation="MRRHGGFTLVELLAGIAIVAVLAVLIASAYKNILNSSGAARCVT
                     NMRSLHASFAAYISDNDHWPQQPEFEPDEDEAYGEWWITTMHPYVQSDSIWICPTLQS
                     FGRMNPSSNQMRINYTPTLFDAFRNRPYQWPNMPWLIEAGNAHGKGANVLFPDGSVRT
                     YSDLLDSYQNAQ"
     gene            1735..>3101
                     /locus_tag="VIS74_01125"
     CDS             1735..>3101
                     /locus_tag="VIS74_01125"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEY5791868.1"
                     /translation="MSTPLLSAHELGFSYGNQHLLKAVTLAITAGEKIGLVGRNGCGK
                     TSLLKILSGENQPDRGEVVRKKGLRVGYLPQEFELDGAQSVRANIEAGAADLVEAMQR
                     YEAGNGSDRELEEWHHLIDAGDGWNLDVRIRADASALGVPPLDSLVGSLSGGEKRRVA
                     LCRALVSQPDLLLLDEPTNHLDAESIQWLESFLRNFSGAVIFITHDRYFLDVIATRII
                     ELADGHSYSHPGNYTAYLEAKAQRQEIAGQNERRRQRFLRAELSWVRAGVKARTTKSK
                     HRLQSYYEVAGQEAPPEERDLDLLIPPAPEVGNIGIELIDAGARVDGSRWLFRHLNLS
                     LRPGECTGIVGRNGVGKTTLLRICLGERAPDEGAATLGKKIQFNYIDQNRLQLDGRGT
                     VLSEVEDSDGFVHFGDQKLSVRAYLRRFLFSDARINERAERLSGGERARLLLAKVLKR
                     GGNLLV"
BASE COUNT          654 a          985 c          864 g          598 t
ORIGIN      
        1 tcaggttcac caccccccag ccgcagcgga tcagcagctg gaaattttcg accagcagct
       61 tgccaatgcc aaatcccacc acgatcaaag ccagccaata ggcggcggcg cccagcaaaa
      121 agtaggaata acccgccagc aacgccccga cgacgcccac taaattgtgc agcggctcat
      181 tttgctgcga ggtcgaataa atcagcggcg cccagccggg cagatccccc ttatcgaagg
      241 aaatcagcgc cacaaacagc aaaactccga ggaggatcag gccgatgccg atgacttcct
      301 tccagggatt ggttttccgg aaacgattca tgaatggaaa cgaattttat gcaccatcgc
      361 cattcgcgcg acaagggaag cgtggaaatt ttctggcgaa aaaacgcgcc gccccgcgcg
      421 aaaatgactt ttccgaacca cgccccaaat tcaggcttga ccctgaggcc gcgatccccc
      481 aaaacgccaa aatccattat gaagattctc cctctagcgc tcttcgccac cctcctcgga
      541 gcagccagcg gcttcgccgg ctcctttggc cccccgcctt tcagcaacgg ctcccccctg
      601 caaagcggca cggatggcac ttatcaagcc acgttgagcg gaaccaacat gaccggcatc
      661 ctcagttttg cgatgcaaaa cggcagccag accggttccg ggtcgcagaa tcaatgggtc
      721 gcatttcaac atggcgctgt ttttcgtggg aatgttcaag ccaacatggt caaaacccag
      781 attaccggca tcctcgattc ggccacgctt gagctgcctg aggatgacga tggcaggatc
      841 gaattgcccg tagtttttct cattcagggg aatagagcgt ccggagcatt tacggccaac
      901 atcgattttg aagaccctta cggcaaattc actggccagg gccacattga ggggagcacc
      961 tccgactcga tcactatctt tggcatcttc accaacgtct ttggagacca gggcaccatc
     1021 accttctcta ccaatctgcc gtccggcacc gtgcagcgag ctgctttcag acttcgcggc
     1081 actcgcacca gcacgtcggc ttcgggcact gccggcagct cgaccaacgg ttaatttcct
     1141 gaaactccaa tcagtcccaa gggctttgtc tcaagggagt cgaattcatt gcgaagacac
     1201 ggcggattta ccttggtgga gttgctggct ggcatcgcga tcgtcgccgt gctggcggtt
     1261 ctgattgctt ccgcttacaa gaacatcctt aactcgtcgg gcgcggcccg ttgcgtgacg
     1321 aacatgcgaa gcctgcacgc ttcgtttgcc gcctatattt cggataacga tcattggccg
     1381 caacagccgg agttcgaacc ggatgaggac gaagcctatg gagaatggtg gatcaccacc
     1441 atgcacccct acgttcagtc cgattccatt tggatctgtc ccaccttgca gtccttcggc
     1501 cggatgaatc cgagcagtaa tcaaatgcgg atcaactaca ctcccaccct gttcgatgcg
     1561 tttcgcaacc ggccttacca gtggcccaac atgccctggc tgatcgaagc cggaaacgcc
     1621 catggcaagg gggctaacgt gctgtttccc gacgggtccg tccggaccta ttccgacctg
     1681 ctcgatagct accaaaacgc ccaatagcca ccctggctgt tgagctccgt tcctgtgagc
     1741 actcctctcc tttccgccca cgagctgggt ttttcctatg ggaatcaaca cctgctcaaa
     1801 gcggtcactc tcgccatcac cgcgggcgaa aaaatcggcc tcgtcgggcg caatggctgc
     1861 ggcaagacca gcctgctgaa gattctctcc ggagaaaacc agccggatcg cggcgaagtc
     1921 gttcggaaga agggcctgcg cgtgggttat ctgccgcagg aattcgaact cgacggcgcc
     1981 caatccgtcc gggcaaatat cgaagccggc gccgccgatc tggtggaagc catgcagcgt
     2041 tatgaagccg gaaacggttc ggaccgggaa ttggaggaat ggcatcacct catcgatgcc
     2101 ggggatggct ggaacctcga cgtccgcatc cgcgcggatg ccagcgcgct gggcgtgccg
     2161 ccgctcgact cgctcgtggg ctcgctgtcg ggcggggaaa agcgccgtgt cgccctctgc
     2221 cgcgcgctgg tgtcccagcc ggatttgctg ctgctcgacg agccgaccaa ccacctggac
     2281 gccgagtcca ttcaatggct ggaaagcttc ctgcgaaact tttccggggc ggtgattttc
     2341 atcacccacg accgctattt tctcgatgtc atcgcgacgc ggatcatcga gctcgccgat
     2401 ggccacagct actcgcatcc gggcaattat accgcctacc tggaagcgaa ggcccagcgg
     2461 caggagatcg ccgggcagaa cgaacgccgc cgccagcgtt ttctccgggc ggaattgtcc
     2521 tgggtgcgcg ccggggtcaa ggcgcggacc acgaaatcca agcaccggct gcagagttat
     2581 tacgaagtgg ccgggcagga agccccgccg gaggagcgcg atctggattt attgatcccg
     2641 cccgctccgg aagtgggcaa tatcggcatc gaactcatcg acgcgggcgc gcgggtggac
     2701 ggctcgcgct ggctgtttcg ccatctgaat ctctcgctgc ggcccggcga atgcacgggc
     2761 atcgtcggcc gcaacggcgt cggcaaaacc accctgctgc gcatctgcct cggcgaacgc
     2821 gcgccggatg aaggcgccgc cacgctcggg aagaagattc agtttaatta catcgatcaa
     2881 aaccgcctgc agctcgacgg acgcggcacg gtgctctcgg aggtcgagga cagcgatggt
     2941 tttgtgcact tcggcgatca aaaactcagc gtccgcgcct atctccgccg gtttcttttc
     3001 tccgacgccc gcatcaatga acgcgccgaa cggctctccg gcggcgaacg cgcgcgcctg
     3061 ctgctggcca aggtgctcaa gcgcggcggc aatctgctgg t
//
LOCUS       DATDAY010000056         2026 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_72281, whole genome shotgun sequence.
ACCESSION   DATDAY010000056 DATDAY010000000
VERSION     DATDAY010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2026
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_72281"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..673)
                     /locus_tag="VIS74_01130"
     CDS             complement(<1..673)
                     /locus_tag="VIS74_01130"
                     /inference="COORDINATES: protein motif:HMM:NF014912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HEY5791869.1"
                     /translation="MAKRFTDLSEREILALAISLEEEDSRIYADFADRLKESYPSTAE
                     MLRAMTLEEREHARKLTACFRENFGEHVPLIRRGDVRNFIQRKPIWLLHSLSLEQIRR
                     EVHSMENESQRFYRHAAERASGAPVQNLLESLAQEEDNHLALAEKFTGKLEASGAVKR
                     EAETERRTFVLQVIQPGLAGLMDGSVSTLAPVFAAAFATRDSWDAFIVGMAASIGAGI
                     SMGFAE"
     gene            complement(685..>2026)
                     /locus_tag="VIS74_01135"
     CDS             complement(685..>2026)
                     /locus_tag="VIS74_01135"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006983402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HEY5791870.1"
                     /translation="IYVVLNMRLMTRMGTVAYQQLGDSGDDFNRGIHSVLDCHPDRRL
                     ICHFPQDNTVISVGSGYGGNALLGKKCLALRIGSYLGQQQGWLAEHMLLLCVKSPKGQ
                     KTYVAAAFPSACGKTNFAMMIPPKEFAGWEITTIGDDIAWMKPGEDGRLYAINPEAGY
                     FGVVPGTNAKSNPNAMRSMERNVIFTNVGLTTDGDIWWEGKDNPPPPNLLDWRGDPWT
                     PESGEKCAHPNSRFTAPMRNNPALAAEADDPDGVPIQAIIFGGRRATTMPLVFQAFNW
                     IHGVYIGATVGSEMTAAAVGGQGQVRRDPMAMLPFCGYNMGDYFSHWLEMRQAIKYIP
                     RIFHVNWFRKDKDGKFLWPGFGENMRVLKWIVDRCRSGAHALETSVGWVPQYDDIDLT
                     GLDEVTREKFEELQHIDSEEWKREILLQDELFMSLYSSLPKELIFQRELLAARL"
BASE COUNT          379 a          624 c          622 g          401 t
ORIGIN      
        1 cctcggcaaa tcccatgctg atcccggcgc cgatggatgc ggccattccc acgatgaagg
       61 catcccagct atcccgggtg gcaaaggccg cggcgaaaac cggggccagc gtcgagacgg
      121 agccatccat caaccccgcc aagccgggtt ggatgacctg cagcacaaac gttcggcgct
      181 ccgtctccgc ctcgcgcttc acggcgccgc tggcctccag cttgccggta aatttttccg
      241 ccagcgcaag atggttatct tcttcttggg caaggctttc aagcagattt tgaacgggcg
      301 cacccgaagc ccgttccgcc gcgtggcgat agaaccgctg ggattcgttt tccatggaat
      361 gcacctcgcg ccggatttgc tccagcgaaa gggaatgcag cagccagatc ggcttgcgct
      421 gaatgaagtt ccgaacatcg ccccggcgga tgagcggcac atgctcccca aagttctcgc
      481 gaaaacacgc ggtgagcttg cgggcgtgct cacgctcctc cagggtcatg gcgcggagca
      541 tctccgcggt ggagggatag gattccttca gccgatccgc aaaatcggcg taaatccggg
      601 aatcctcctc ctccagcgaa atggccagag ccaggatttc gcgctcactg agatcggtaa
      661 accgtttggc catggctcgc ggttttacag ccgggcggcc aaaagttcac gctggaagat
      721 cagctccttc ggcaggctgc tgtagaggct catgaacagc tcgtcctgaa gcaggatctc
      781 gcgtttccat tcctccgaat cgatgtgctg gagctcctcg aatttctccc gcgtgacctc
      841 atcgagcccg gtcaggtcga tgtcatcata ctgcggaacc cagccgaccg aggtttccag
      901 cgcgtgcgcg ccgctgcggc agcggtccac gatccatttc agcacccgca tattttcccc
      961 gaagccgggc caaaggaact tgccgtcctt gtccttgcgg aaccagttca cgtggaaaat
     1021 gcgcgggatg tatttgatcg cctggcgcat ctcgagccag tgcgagaaat agtcgcccat
     1081 gttgtagccg caaaagggca gcatcgccat cgggtctcgg cgcacctggc cctgtccgcc
     1141 cacggcggcg gcggtcattt ccgaacccac cgtcgcgccg atgtaaacgc catggatcca
     1201 attgaatgcc tggaagacaa gcggcatggt cgtggcgcgg cggccgccga aaatgatcgc
     1261 ctggatcggc acgccatcgg gatcatccgc ctccgcggcc agcgcgggat tattgcgcat
     1321 gggcgcggta aagcggctgt tgggatgcgc gcatttttcg ccgctctcgg gagtccaggg
     1381 gtcgcccctc cagtcgagca ggttgggggg cgggggatta tccttgccct cccaccagat
     1441 gtcgccgtcg gtcgtcagtc cgacgttggt gaaaatgacg ttgcgctcca tggaccgcat
     1501 ggcgttggga ttcgacttcg cattcgtgcc gggcacgacg ccgaagtagc cggcttcggg
     1561 attgatggcg taaagccgtc cgtcctcgcc gggcttcatc cacgcgatgt catcgccgat
     1621 ggtggtgatt tcccatcccg cgaactcctt cggcggaatc atcatggcga aattcgtttt
     1681 gccgcaggcc gacgggaagg ccgccgccac gtaggttttt tggcccttgg gggattttac
     1741 gcagagcagc agcatgtgtt ccgccagcca gccctgttgc tggccgagat aggagccgat
     1801 gcggagggcg aggcattttt tgcccagcag ggcgttgccg ccgtagccgg agccgacgga
     1861 gatgacggta ttatcctgcg gaaaatggca gatcaagcgg cggtcggggt ggcagtccag
     1921 cacgctgtga atgccgcgat tgaagtcgtc acccgaatcc cccagttgct gataggcgac
     1981 ggtgcccatt cgggtcatca gccgcatatt cagcacgaca taaatg
//
LOCUS       DATDAY010000057         2206 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_208536, whole genome shotgun sequence.
ACCESSION   DATDAY010000057 DATDAY010000000
VERSION     DATDAY010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2206)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2206)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2206
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_208536"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..706)
                     /locus_tag="VIS74_01140"
     CDS             complement(<1..706)
                     /locus_tag="VIS74_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEY5791871.1"
                     /translation="MNSFFIFLITAAFTFGGMFLILPGLLALLRLLGGYVIVQERRAH
                     VYVLFGRVIGVIDQPGFHILLFRLGPAALIVHWLGQCRVIDLRIDQVYLRSQPVNSEE
                     GAPMGIGIWYEMFISDPVAYLFKNSDPRGSLAANVSSATVRCLSNMKLTDMLENRHEM
                     SRAVRTEASASSKEWGYDLGSIYIRKVHFRDAGMMLQIESKVVNRLRQVTSAIRQDGA
                     NQVSVITSTAERQAAVE"
     gene            complement(699..1697)
                     /locus_tag="VIS74_01145"
     CDS             complement(699..1697)
                     /locus_tag="VIS74_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEY5791872.1"
                     /translation="MAVILGIIIGFVSWFLVRYLIAGLYTVNQDERAVKTSFGRAERI
                     GTGSILDDPIAATLTPEERARYGAPLLRVIPPGGPYFRWLWEKIYKVNVSTQTINMAF
                     DPETPEANQHGTVLDAVTKDQLNTGLTGQIRYRIAERNLYAYLFGVRNPIVHVMGYFI
                     SVLRERIANFEAPPAPPAPGGETTPESALSVAGVSINDLRKNLRDLNDHMERECASSV
                     ARYGIQLEASLITGIDPPAEVESALAAINTAYNQVSSDISLAHATADQTIVLSKRAVE
                     IETLKVHAELEPLTALADQLQTLKSHGKNVLEAYRRSVRLRLFENARRAIVEVDHE"
     gene            complement(1957..>2206)
                     /locus_tag="VIS74_01150"
     CDS             complement(1957..>2206)
                     /locus_tag="VIS74_01150"
                     /inference="COORDINATES: protein motif:HMM:NF022479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2721 domain-containing protein"
                     /protein_id="HEY5791873.1"
                     /translation="RTRIKWVRNMQFFGILSLLFCTVCVFALFFEQLFGAVILFCASV
                     ISMIVSLVYSLLEIRLSVKAIDLHLSDLESKINSRGHQ"
BASE COUNT          441 a          613 c          737 g          415 t
ORIGIN      
        1 actcgaccgc cgcctggcgc tcggcggtgc tcgtgatgac gctcacctga ttggccccgt
       61 cctgccggat ggcggaagtc acctggcgga ggcggttcac gactttgctc tcgatttgca
      121 gcatcatgcc cgcgtcgcgg aagtggacct tgcggatgta aatcgaaccg aggtcgtagc
      181 cccattcctt cgaggaggcg ctggcttcgg tgcggacggc gcggctcatc tcatgccggt
      241 tctccagcat gtcggtgagc ttcatgttgc tgaggcagcg gacggtggcg ctgctgacat
      301 tcgcggcgag cgaaccgcgg ggatcggagt tcttgaacag ataagcgacc ggatcgctga
      361 tgaacatctc ataccagatg ccgatgccca tgggcgcgcc ttcctcggag ttcaccggct
      421 ggctgcggag atagacctgg tcgatccgca gatcgatgac ccggcattgg ccgagccaat
      481 ggacgatgag cgcggcgggc ccaaggcgga agagcaggat gtggaagccg ggctggtcga
      541 tgacgccgat gacgcggccg aagagcacgt aaacgtgggc gcggcgttcc tggacgatca
      601 cgtagccgcc cagcaggcgc aggagggcga gcaggccggg caggattaaa aacatgccgc
      661 cgaaggtaaa ggcggcggta atgagaaaga taaaaaagct attcatggtc cacctccaca
      721 atggcgcggc gcgcgttttc aaagagccgg agccggacgc tgcggcggta ggcttcgagg
      781 acgtttttgc cgtgggattt gagcgtttgc aactgatccg ccagcgccgt gagcggctcg
      841 agctcggcgt gcaccttgag cgtttcgatt tccaccgccc gcttggagag gacgatcgtc
      901 tggtcggcgg tggcgtgggc gaggctgatg tccgaggaaa cctggttgta ggcggtatta
      961 atggccgcaa gcgcggactc cacctccgcg ggcggatcaa tgccggtgat gagcgaggct
     1021 tcgagctgga tgccgtagcg ggcgacggag gaggcgcatt cgcgttccat gtggtcgttc
     1081 aggtcgcgga ggtttttgcg cagatcgttg atggagacgc cggcgacgct gagcgcggac
     1141 tcgggggtcg tttcgccgcc cggcgcgggc ggggccggcg gggcctcgaa gttcgcgatg
     1201 cgctcgcgca ggacactgat gaagtagccc atgacgtgga cgatgggatt ccgcaccccg
     1261 aagaggtagg cgtagagatt ccgctccgcg atgcggtagc ggatttgtcc ggtgaggccg
     1321 gtattgagtt gatccttcgt caccgcgtcg agcacggtgc cgtgctggtt cgcctcgggc
     1381 gtttcgggat cgaacgccat gttgatggtt tgggtggaga cattgacctt gtaaatcttt
     1441 tcccacagcc accggaaata cggaccgccg ggcgggatca cgcgcagcag cggcgccccg
     1501 tagcgcgcgc gttcctcggg cgtgagcgtg gcggcgatcg ggtcatcgag aatcgatccc
     1561 gttccgatgc gttcggcccg gccgaacgaa gtcttcaccg cgcgctcatc ctgattcacg
     1621 gtgtaaagtc cggcgatgag ataccggacg agaaaccaac tcacaaaccc gatgataatt
     1681 ccgagaatga cggccatagc tttatagcca acaggattac aggatccccg gaatgaacag
     1741 aattattttg agattatttt gactcattcc gttcatccgg aaaatcccgg tccgaagcgg
     1801 gcggcaagag ccggccggcc tctcggtgga tacgaacgta aatccttgga gaaagccacg
     1861 gcgggttcat cgaacccgcc ctcccaaccc ttgggtaggg acggctcgcc gggccgtccg
     1921 tgattttccg ttaaatcggt ctcgacccaa acggctctac tggtgccccc gtgagttgat
     1981 cttgctttcg agatcgctga gatgcaggtc gatggccttt accgacagcc ggatttccag
     2041 cagggaataa accagcgaaa caatcatgga aatcacgctg gcgcaaaaga ggatgaccgc
     2101 gccgaagagt tgctcgaaga aaagggcgaa gacgcaaacc gtgcaaaaca gcaggctgag
     2161 gatgccgaaa aattgcatgt tccgaaccca cttgatgcgc gtccgc
//
LOCUS       DATDAY010000058         3832 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_1977543, whole genome shotgun sequence.
ACCESSION   DATDAY010000058 DATDAY010000000
VERSION     DATDAY010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 3832)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3832)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3832
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1977543"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..793)
                     /locus_tag="VIS74_01155"
     CDS             complement(<1..793)
                     /locus_tag="VIS74_01155"
                     /inference="COORDINATES: protein motif:HMM:NF027219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc-binding peptidase"
                     /protein_id="HEY5791874.1"
                     /translation="MKIFHCDHCQQLVHFENTLCVNCGRTLAFLPDLGEMGSLDLLGN
                     GLWSSAHPYAQGRTYRLCQNYAVESVCNWALPAGNPEIYCQSCRLTHVIPDLMRFGNK
                     QAWAKLETAKRRLIYSLLELHLPVLSKADDARRGLAFEFLADPDFPTETPIFTGHAEG
                     HITINLTEADDAERERRRHDLGEPYRTLLGHFRHEVGHYYWDRLIAGTGRLEPFRALF
                     GDERPDYDEALKRHYELGAPADWQMRFITPYASTHPWEDWAESWAH"
     gene            complement(832..1266)
                     /locus_tag="VIS74_01160"
     CDS             complement(832..1266)
                     /locus_tag="VIS74_01160"
                     /inference="COORDINATES: protein motif:HMM:TIGR00028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system VapC family ribonuclease toxin"
                     /protein_id="HEY5791875.1"
                     /translation="MQPIDLLDVNVWLALAVEDHPHHERARHYWEQEGAPCLAFCRIT
                     MLGLLRLSTHSQIMAGHPFSPAEAWTMYRGFRALPEIRFLPEPPELEADFAELSGAVS
                     FSAALWTDAYLASLARLADCRLISFDKDFAKFPDLTFLQLRV"
     gene            complement(1253..1429)
                     /locus_tag="VIS74_01165"
     CDS             complement(1253..1429)
                     /locus_tag="VIS74_01165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5791876.1"
                     /translation="MKIKELLEIYVREGLREPVFASIRRSPLPPPRPRTGHSLPALAN
                     AEIQQILNTEDAAH"
     gene            1706..2491
                     /gene="ccsA"
                     /locus_tag="VIS74_01170"
     CDS             1706..2491
                     /gene="ccsA"
                     /locus_tag="VIS74_01170"
                     /inference="COORDINATES: protein motif:HMM:NF013725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HEY5791877.1"
                     /translation="MNRLLLAAAALCFLLSFGYTLFALGAGRFRPGRFNLLAMLGGFA
                     GQLAYLYLRGLEVQSCPLHTLFDLLIFLSWSITLIYLVVGPAYRLSLLGAFTSPLVLV
                     LTLIALVLPHPPAAPTPFANPWVEFHAALSIIAYGCFGLASVAGVMYLLQERQLKNRK
                     ASALLYHLPPINDLATANKRLVSLGLILLTVAFAAGFVSGLPIVGLKFWASTLIWLLY
                     GGLLLLWRAHALAPRRIATLSVGVFAFALILLPGIHYLSLQRL"
     gene            2488..3588
                     /gene="hemA"
                     /locus_tag="VIS74_01175"
     CDS             2488..3588
                     /gene="hemA"
                     /locus_tag="VIS74_01175"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: protein motif:HMM:TIGR01035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HEY5791878.1"
                     /translation="MNIFCAGINHRTAPVEIRERFAVLPHEMGSVLQEIRGFDSLHEA
                     VVLSTCNRVEFYTTSICPERARTDLEKWMERRAGFRPPIYWHDTARSVRHLFRVSCGL
                     DSMVLGETEVLGQIKQAYSHATEAGTTSRHLNKLFQHAFRVAKNVRTHTQITRGATSV
                     GSVAVELATKIFGHLQGRRVMILGAGETSERTARSLQSRGIQSVIVSNRTFDRAARLA
                     EEIGGMAIHFDHWHKAFHEVDILISSTAAPHPILTKEKLAPMMRDRADRPLFVIDLAV
                     PRDAEPAINELEGVFLYDIDSLKEIAGNSRQVRQQEIQRCEQLIDHNVQDFVGWMRRL
                     HHGEFSIGDFRFSNEDAGSRPSKIENRKSRLP"
     gene            3585..>3832
                     /locus_tag="VIS74_01180"
     CDS             3585..>3832
                     /locus_tag="VIS74_01180"
                     /inference="COORDINATES: protein motif:HMM:NF013538.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5791879.1"
                     /translation="MIVLGTRGSDLALAQTRLVQTALRQAHPEWVVESRIIHTQGDRR
                     LDVSLPEAGALDKGLFTKELEEALLRGEIHGAVHSLKD"
BASE COUNT          746 a         1230 c         1107 g          749 t
ORIGIN      
        1 aatgcgccca gctttccgcc caatcctccc agggatgggt gctcgcgtag ggcgtaatga
       61 accgcatctg ccaatccgcc ggagcgccca actcataatg ccgctttaat gcctcgtcgt
      121 aatccgggcg ctcatcgcca aagagcgcgc ggaagggctc cagccggccg gtgccggcga
      181 tgaggcgatc ccaataataa tgccccacct cgtgccggaa atgcccaagc agcgtgcgat
      241 acggctcacc cagatcatgc cggcggcgct cgcgttccgc gtcgtcggct tccgtgagat
      301 tgatggtaat gtgcccctcg gcatggccag tgaaaatggg cgtttccgtg ggaaaatccg
      361 gatcagccag gaattcgaac gccaggccgc gccgggcgtc gtcggccttg ctgagcaccg
      421 gcagatggag ttccaacaga ctgtaaatca accgccgctt ggcggtttcc agcttggccc
      481 acgcctgctt gttgccgaag cgcatcagat cgggaatgac gtgcgtcaac cggcaggact
      541 ggcaataaat ctccggattg ccggccggca gcgcccagtt gcaaacggac tccaccgcgt
      601 aattctgaca aagccggtag gtgcgcccct gcgcgtaggg atgcgccgaa ctccaaagac
      661 cattgcccag caaatcgagc gagcccattt cccccagatc gggcaggaac gccagcgtgc
      721 ggccacagtt cacgcacagc gtgttctcaa aatggacaag ctgctggcag tggtcgcaat
      781 ggaagatctt catgggaagg atgaaatcgg ccgcaggaag gaatcagtcg tttaaacccg
      841 cagctgaagg aaggtcaaat ccggaaattt tgcaaaatcc ttatcaaagg aaatgaggcg
      901 gcaatccgcc agccgcgcca gcgaggcaag ataggcatcg gtccaaagag ccgctgaaaa
      961 cgaaaccgct ccactcaatt ccgcaaaatc ggcctccaat tccggcggct cgggcaagaa
     1021 tcgaatctcc ggcaatgccc taaagccgcg atacatcgtc cacgcttccg cgggactgaa
     1081 aggatgcccg gccatgattt gggaatgcgt gctcaaacgc agcaggccca gcatcgtgat
     1141 ccggcaaaaa gccaggcagg gagccccttc ctgttcccaa taatgccggg ctcgctcatg
     1201 atgggggtgg tcttcaaccg cgagagccag ccagacatta acgtccagca aatcaatggg
     1261 ctgcatcttc cgtgttcagg atttgctgaa tttccgcatt ggccagcgcc gggagcgaat
     1321 gcccggttcg tggccggggc ggtggcagcg ggctgcgtcg aattgaagcg aagaccggct
     1381 cacgcagacc ttcccggacg taaatctcaa gtaattcctt gattttcaat ccttccttgg
     1441 cggcgcggct cttcaaatcc cgaaaaagcg gatcaggtaa atccaaggtt gtgcgcataa
     1501 aagcataaaa gcatcttaat gtttttatgt caatggactc attattgaag gacttctgca
     1561 gcccggaatc ctcatttcaa aatttcaaaa aattcaatct tcattccggt taatccggca
     1621 aatccagtaa tcccgtttag ttgactccat gctcgccgcg aacttgcccc tttcgcttcg
     1681 ccggtttttc tgcgattaga gaaaggtgaa ccgcctcttg cttgccgccg ccgcgctctg
     1741 ctttttgctg agttttggct acacgctgtt cgcgctcggg gcgggccggt ttcggccggg
     1801 ccggttcaat ctcctcgcca tgctgggcgg ttttgccggg cagttggcgt atctctacct
     1861 gcgcgggctg gaagtccaaa gctgcccgct gcacacgctt ttcgacctgc tcatcttcct
     1921 cagctggtcc atcacgctga tttatctcgt ggtcgggccg gcctaccgcc tgtcgctgct
     1981 gggcgcgttc acctcgccgc tggtgctggt gctgacgctc atcgccctcg tgctgccgca
     2041 cccgcccgcg gctcccactc cgtttgccaa tccctgggtg gaattccacg cggcgctttc
     2101 gatcatcgcc tacggctgct tcgggctcgc gagcgtggcc ggagtcatgt atctgcttca
     2161 ggaacggcag cttaagaatc gcaaggcttc ggcgctgctc taccatcttc cacccatcaa
     2221 tgatctcgcc accgcgaaca agcggctcgt cagcctcggg ctgatcctct tgaccgtcgc
     2281 cttcgccgcg gggttcgtct ccggcctgcc catcgtgggc ctgaagttct gggcgtccac
     2341 cctcatctgg ctgctttacg gcgggctgct gctgctctgg cgcgcccacg cgctggcgcc
     2401 gcggcgaatt gccacgctgt ccgtcggcgt ttttgcgttt gcgctgatcc tgcttccggg
     2461 cattcattac ttatccttgc aacggctgtg aacatcttct gcgcaggcat caaccaccga
     2521 accgctcccg tggaaatccg ggaacgcttc gccgtgctgc cgcacgaaat gggctccgtc
     2581 ctgcaggaaa tccgcggctt cgattcgctg cacgaagccg tcgtgctttc gacctgcaat
     2641 cgcgtcgaat tttacaccac cagcatctgc cccgagcgcg cccggaccga cctggagaaa
     2701 tggatggagc gccgcgcggg gttccggccg ccgatttatt ggcacgatac cgcccgcagc
     2761 gtgcggcacc ttttccgggt ctcctgcggg ctggattcca tggtgctcgg cgaaaccgag
     2821 gtgcttggcc agatcaagca ggcgtattcc cacgcgaccg aggccgggac cacctcgcgg
     2881 cacctcaaca agctcttcca gcacgccttc cgcgtggcca agaatgtccg cacccacacg
     2941 cagatcacgc gcggggcaac ctccgtcggc tcggtcgccg tggagctggc gaccaaaatc
     3001 ttcggccacc tgcagggccg gcgcgtgatg attctcggcg ccggggagac cagcgaacgc
     3061 acggcgcgca gcctgcagtc ccgcggcatc cagagcgtga tcgtctccaa ccgcaccttc
     3121 gaccgcgccg cccggctggc cgaggagatc ggcggcatgg cgatccactt tgaccattgg
     3181 cacaaggcgt tccacgaggt ggacatcctc atcagctcca ccgccgcccc gcatccgatc
     3241 ctgacgaagg aaaagctcgc gccgatgatg cgcgaccgcg ccgaccggcc gctgtttgtc
     3301 atcgacctcg ccgtgccgcg cgacgcggag ccagccatca acgaactcga gggcgtgttc
     3361 ctttacgaca ttgattcgct caaggaaatc gccgggaatt cgcggcaggt ccgccagcag
     3421 gaaatccagc gctgcgagca gctcattgat cacaacgtcc aggacttcgt cggctggatg
     3481 cggcgattac accacgggga attttcgatt ggcgatttcc gattctcgaa cgaagacgcc
     3541 gggagccgcc catcgaaaat cgaaaatcga aaatcgaggc tcccatgatc gttctcggaa
     3601 cccggggcag cgacctcgcg ctggcgcaaa cccggctggt gcaaaccgcg ctgcggcagg
     3661 ctcatccgga gtgggtggtc gagtcgcgca tcattcacac ccagggcgac cgccggctcg
     3721 atgtgagcct tcccgaggcg ggggcgctcg ataaggggct cttcaccaag gaactcgaag
     3781 aggcgctgct gcgcggggaa atccacggcg cggtgcacag cctgaaggac tt
//
LOCUS       DATDAY010000059         2951 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_317473, whole genome shotgun sequence.
ACCESSION   DATDAY010000059 DATDAY010000000
VERSION     DATDAY010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2951)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2951)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2951
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_317473"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..476)
                     /locus_tag="VIS74_01185"
     CDS             complement(<1..476)
                     /locus_tag="VIS74_01185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5791880.1"
                     /translation="MVKFLWLFAALAALTPLRAGTDCNARVLQTLPHLPVGGGYATTS
                     EASARLRAAVNAAGGDLVLMPKRAQPSYCSGATYLVFLNAVRPNLTPPAAAALADMEK
                     ADGHGVWGRWNANGPGTARLFYELGLGRNFTDLAEARPGDFLKIFWRDAVGKNEFG"
     gene            558..1193
                     /locus_tag="VIS74_01190"
     CDS             558..1193
                     /locus_tag="VIS74_01190"
                     /inference="COORDINATES: protein motif:HMM:NF024919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine peptidase family C39 domain-containing
                     protein"
                     /protein_id="HEY5791881.1"
                     /translation="MTFRKTAGLLLAALLICAAYWAWTWTRPGPLSPSGGLYFPARVE
                     IPLPHFAQADPRWGGLPLGSTPSTIAGEGCAVAAAAMVLAGYGAAIDPGKLNAFLTAR
                     PGGYTPEGWIYWEKAAEIDPALTAKLLPHYEDAGSYFWLDWNLFRGNPVIVRLRYGNG
                     TTHFVVVVGKQGRDYLVLDPGHGFGKGVYPLREFGSDIEAIRFYRLPSTRG"
     gene            1371..2519
                     /gene="moeB"
                     /locus_tag="VIS74_01195"
     CDS             1371..2519
                     /gene="moeB"
                     /locus_tag="VIS74_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_257790190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HEY5791882.1"
                     /translation="MEFSNDEVARYSRHLIMPEVTLEGQKRIKAAKVLCIGTGGLGSP
                     IALYLAAAGVGTLGLIDFDVVDFSNLQRQVLHGTKDVGRKKLNSARDRIRDINPNVQV
                     VLHDCLFNSENAREIVEPYDIVIDGTDNFPTRYLSNDICVMLKKPNIYGSIFRFDGQA
                     TVFAPHLGGPCYRCLYPEPPPPGMVPSCAEGGVLGVLPGVIGVMQAIEAVKLICGIGE
                     PLIGRLIHFDALKMKFREFKVRKDPSCPLCGEHPTLTELIDYEQFCGIPQAQAAEEAE
                     PPVPEISVTALKAKLDAGRVVLVDVREPFEYDIARIPGAKLIPLGDLPSRMSELDSAD
                     EIYIHCKSGVRSANALRQLQKAGFGKLYNVTGGILAWADQVDSSVPKY"
     gene            2532..2945
                     /locus_tag="VIS74_01200"
     CDS             2532..2945
                     /locus_tag="VIS74_01200"
                     /inference="COORDINATES: protein motif:HMM:NF017833.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide biosynthesis protein"
                     /protein_id="HEY5791883.1"
                     /translation="MAAPVSRRLSEELTALREGVLGRGVSLGEMMDHLQGRGFMLFLI
                     LLVAPFCQPVPLPGLSMLFGIVVAFIGLRITLRQKPWLPERLRRVVISGRTMGKLIGA
                     AVWLIKWIEKWSRPRTVEHFEGRWTHSLYGIVVFL"
BASE COUNT          530 a          960 c          899 g          562 t
ORIGIN      
        1 tgcccaaact catttttccc caccgcgtcg cgccagaaaa ttttcagaaa gtcccccggc
       61 cgcgcctcgg ccaggtcggt gaagttccgg cccaggccca gctcgtagaa caatcgggcc
      121 gtgcccggcc cattggcgtt ccaccggccc cagacgccgt ggccgtcggc tttctccatg
      181 tccgcgagcg ccgccgcggc gggcggcgtc agattcgggc ggaccgcgtt gagaaacacc
      241 agataagtcg ctcccgaaca gtagcttggc tgggcgcgtt tcggcatcag gacgagatcg
      301 ccgccggcgg cgttcacggc cgcccgcagg cgggccgagg cctcggaagt ggttgcatat
      361 ccgccgccga ccggaaggtg cggcaatgtt tgcagcaccc gggcattgca atccgtcccc
      421 gcccggaggg gagtcagggc ggccagtgcg gcgaatagcc aaaggaattt aaccacagag
      481 gacacaaaga gcacagagat cgaatgaaat ccaaaaaaat cctttcccct cggtatcctc
      541 tgtgccctct gtgagaaatg actttccgaa aaaccgccgg tctcctgctc gcggcgttgc
      601 tgatttgcgc cgcatattgg gcctggacgt ggacgcggcc cggcccgctt tcgcccagcg
      661 gcgggctgta ttttccggcc cgcgtcgaga ttccgctgcc gcatttcgcg caggccgatc
      721 cgcgttgggg cggcctgcca ctgggctcga ccccttcgac gattgccggc gaaggctgcg
      781 ccgtcgccgc ggcggccatg gtgcttgcgg gttacggagc cgccatcgac cccgggaaac
      841 tcaatgcctt cctgaccgcc cggcccggcg gctacacgcc ggagggctgg atttattggg
      901 aaaaagccgc ggagatcgat cccgccctga cggcgaagtt gctgccgcac tacgaggacg
      961 cgggctccta tttctggctg gattggaacc tttttcgcgg aaacccggtg atcgtccgcc
     1021 tgcgttatgg caatggaacc acgcattttg tcgtggtggt cggcaagcag ggccgcgatt
     1081 acctcgttct cgatcccggc cacggcttcg gcaagggcgt ttaccccctg cgcgaattcg
     1141 gaagtgacat cgaggccatc cgcttctacc gtcttccctc cactcgcggt taagcccgaa
     1201 gggaattaac cacaaagagc gcaaagagga gaggccggat ttttgcggct gatccttttc
     1261 ccggcgttct ctgcggccaa tcccaatccg gcatttgcat cggacgcaaa ccgcatttaa
     1321 cctatacgtc ttttatggag agcatcattg acccgtcccg gttggaggaa atcgaattca
     1381 gcaatgacga agtggcgcgt tattcgcgcc acctcatcat gccggaagtc accctggagg
     1441 gacagaaacg catcaaggcc gcgaaggttc tgtgcatcgg caccggcggt ctcggctcgc
     1501 ccatcgcgct ttatctcgcc gccgccggcg tgggcacgct cgggctgatc gatttcgacg
     1561 tggtggattt ctccaatctc cagcgccagg tgctccatgg caccaaggac gtgggccgca
     1621 agaaactcaa cagcgcgcgc gaccgcatcc gggacatcaa tcccaatgtc caggtcgtgc
     1681 tccacgactg cctgttcaac agcgaaaacg cgcgcgaaat cgtcgagcca tacgatatcg
     1741 tcatcgacgg caccgacaat tttcccacgc gctatctgtc gaacgacatc tgcgtgatgc
     1801 ttaaaaagcc gaacatctac ggatccattt tccgcttcga cggccaagcc acggttttcg
     1861 ccccgcacct cggcgggccg tgctaccgct gcctctaccc cgaaccgccg ccgccgggca
     1921 tggttccgag ctgcgccgaa ggcggcgtgc tgggcgtgct cccgggcgtg atcggcgtca
     1981 tgcaggcgat cgaggccgtg aagctgatct gcggcattgg cgagcctttg atcggccgcc
     2041 tgattcattt cgacgcgctc aagatgaagt tccgcgaatt caaggtgcgc aaggatccgt
     2101 cctgcccgct gtgcggcgag catccgaccc tcaccgagct gatcgactac gagcaattct
     2161 gcggcattcc gcaggcgcaa gccgccgagg aagccgagcc gcccgtgccg gaaatcagcg
     2221 tgaccgccct caaggccaag ctggacgccg gtcgggtggt gctcgtggac gtgcgcgagc
     2281 cgtttgaata cgacatcgcc cgcatccccg gcgcgaaatt gattccgctc ggcgacctgc
     2341 cttcgcggat gagcgagctc gacagcgcgg acgaaatcta cattcactgc aagagcggcg
     2401 tccgcagcgc gaatgccctg cgccagctcc agaaggcggg cttcggcaag ctctacaacg
     2461 tgaccggcgg catcctcgcc tgggcggatc aggtggattc gtccgtgccg aaatactaac
     2521 cggtttccgc ggtggccgcc cccgtttccc gacgactctc cgaagaactg accgctctgc
     2581 gggaaggcgt cttggggcgc ggggtgtcgc tgggggaaat gatggaccac ctgcaggggc
     2641 gcggcttcat gctgtttttg atcctgctcg tggcgccctt ttgccagccg gttccgcttc
     2701 ccggcctgtc catgctcttc ggcatcgtgg tggccttcat cggcctgcgc atcacgctgc
     2761 gccagaaacc ctggctgccc gagcgcctgc gccgcgtcgt gatttccggc aggacgatgg
     2821 gaaaactcat cggcgccgcc gtgtggctga tcaaatggat cgaaaaatgg tcgcgcccgc
     2881 gaaccgtcga gcatttcgag ggcagatgga cgcattccct ttacggcatc gtcgtttttt
     2941 tataggggcg t
//
LOCUS       DATDAY010000060         2237 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_1659611, whole genome shotgun sequence.
ACCESSION   DATDAY010000060 DATDAY010000000
VERSION     DATDAY010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2237)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2237)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2237
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1659611"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..397
                     /locus_tag="VIS74_01205"
     CDS             <1..397
                     /locus_tag="VIS74_01205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5791884.1"
                     /translation="LGKDFSVIRPGKDAEKRVAGRIDLDDNRAVIRYSDTGTTGAYRL
                     FAGPGDLPLAAFAVQLAPSESDLRQAGRPEIEALAQAATAPATTQSATAAAAPRFQVS
                     REFWTWLVCLAALVALAEAALAHRLSQPR"
     gene            411..>2237
                     /locus_tag="VIS74_01210"
     CDS             411..>2237
                     /locus_tag="VIS74_01210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HEY5791885.1"
                     /translation="MTDWIFRIFSSGEKLPEGSQEWSWGAEGIPLGWAFFLFLVLAAA
                     CAWAYRRFAPGAPPRWRRLLAALRIAVIAIFLILLVKPALNLTLHEPVRQALLVLVDA
                     SESMQIQDRRTAPEDLKRAGLATGALDPRLGLNQDAPAGAAAASLNGMSRQQLLNNLA
                     ANEKLNLWPRLFEKSDLSFFRFGRDAMSAGPLAPEDSGSTLTTAEAARFFKALQFKEP
                     ATAIGEATRQVLGESRSQNAGAILLITDGANNSGLPPAEAARMARERDMPLFIYGIGV
                     TESADLHLRDLEAPKLAFAKERVEVKAKLRSQGVTDRSVTATLKANGTEVASQKVEIN
                     ENGDHEITFQFVPEEPGELTLEASTPVLGEEITQENNTASTKIRIVNDKVHVLYIDQE
                     PRWDFRYLLAFLQRDRRLEVQAVLIDGEPGLNQLKDSPFLPGLPEDREGFFKNEILIL
                     GDVDPKDLGETRMQIIREWVEQASGGIIFLAGPKFDPSAYAGTPLEPLLPVVPDTTRS
                     AAQRAERAPEPQSLKLTAAGETSPYLRLNEDPAENQKLWASFPGVRWTAPVERAKPGA
                     QVLLEDPRPDRAGRDGLRPVMAIQGYGSGESVFIGTDETYRWR"
BASE COUNT          395 a          821 c          681 g          340 t
ORIGIN      
        1 gctcgggaag gatttctccg tcatccgccc cggcaaggac gcggaaaagc gggtggccgg
       61 gcgcattgat ctggacgaca atcgggcggt gatccgttat tccgacaccg ggacgacggg
      121 tgcctaccgc cttttcgccg gtccgggcga cctgcccctc gcggcctttg ccgtgcagct
      181 cgcgccatcg gaatccgacc tgcgccaggc cggccgtccg gaaatcgagg cgctcgcgca
      241 ggccgccacc gctccggcaa ccacccaatc cgccaccgcc gccgccgcgc cgcggttcca
      301 ggtcagccgc gaattctgga cctggctggt ctgcctcgcc gcgctggttg cgctggccga
      361 agccgccctg gcccaccgcc tgagccagcc ccgttgaaaa tttcgagacc atgaccgatt
      421 ggattttccg catcttttca tccggcgaaa aactgcccga aggcagccag gaatggagct
      481 ggggagcgga gggcattccg ctgggctggg cgttctttct cttcctcgtg ctcgccgccg
      541 cgtgcgcgtg ggcctaccgc cggttcgccc ccggcgcgcc cccgcgctgg cggcgcctgt
      601 tggccgccct gcgcatcgcg gtgatcgcga tttttctcat ccttctcgtc aaacccgcgc
      661 tgaacctcac cctgcacgag cccgtccgcc aggccctgct cgtgctggtg gatgcctccg
      721 agagcatgca gattcaggac cggcgcaccg cgccggaaga tctgaagcgc gccggcctcg
      781 ccacgggggc gctcgacccg cgcctgggac tcaaccaaga cgcgcccgcc ggcgccgccg
      841 ccgcttcgct gaacggaatg tcccggcagc agctcctcaa caacctcgcc gcgaacgaaa
      901 aactcaatct ctggccgcgc ctcttcgaaa aatccgacct gagctttttc cgcttcggcc
      961 gcgacgcaat gtccgccggc ccgctggcgc ccgaggactc cggctcgacc ctgaccaccg
     1021 ccgaggcggc ccgattcttc aaggccctgc aattcaagga gcccgccacc gccatcggcg
     1081 aagccacccg ccaggtgctc ggcgagagcc gcagccagaa cgccggggcc attctgctga
     1141 tcaccgacgg cgccaacaac agcggcctgc cgcccgcgga agccgcgcgg atggcccgcg
     1201 agcgggacat gccgcttttc atttacggaa tcggggtgac ggagtccgcc gatctccacc
     1261 tgcgcgacct cgaggccccg aagctggcgt tcgccaagga acgcgtcgaa gtgaaggcca
     1321 agctccgcag ccagggagtc accgaccgct cggtcaccgc cacgctcaag gccaatggca
     1381 cggaagtcgc ctcgcagaaa gtcgaaatca acgaaaacgg cgaccacgaa atcactttcc
     1441 aattcgtgcc cgaggaaccc ggcgaactca cgctcgaagc ctccactccc gtcctgggcg
     1501 aggaaatcac ccaggagaac aacaccgcct ccacgaaaat ccgcatcgtg aacgacaagg
     1561 tgcacgtgct ttacatcgac caggagccgc gctgggattt ccgctacctg ctcgccttcc
     1621 tgcagcgcga ccgccggctg gaggttcagg cggtgctcat cgatggcgaa cccggcttga
     1681 atcagctgaa ggactcgccg tttttgcccg gcctgccgga ggatcgcgaa ggatttttca
     1741 aaaatgaaat cctgatcctg ggcgacgtgg atccgaagga tctcggcgaa acgcgcatgc
     1801 agatcatccg cgaatgggtc gagcaggcca gcggcggcat cattttcctg gccgggccga
     1861 aatttgatcc ctccgcgtat gccggcacgc cgctggaacc gctgcttccg gtcgttcccg
     1921 acaccacccg cagcgccgcc cagcgggcgg aacgcgcccc ggagccgcag tcgctcaagc
     1981 tgaccgccgc cggcgaaacc tcgccgtatc tgcggttgaa cgaagacccg gccgaaaacc
     2041 aaaagctctg ggcgagtttc cccggcgtgc gctggaccgc cccggtcgaa cgcgccaaac
     2101 ccggcgcgca ggtgctcctg gaagacccgc ggcccgaccg cgccggccgc gacggcctgc
     2161 ggcccgtcat ggcaatccaa ggctacggct cgggcgagag cgtattcatc ggcaccgatg
     2221 agacctaccg ctggcgc
//
LOCUS       DATDAY010000061         2162 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_2366615, whole genome shotgun sequence.
ACCESSION   DATDAY010000061 DATDAY010000000
VERSION     DATDAY010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2162)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2162)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2162
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2366615"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..391
                     /locus_tag="VIS74_01215"
     CDS             <1..391
                     /locus_tag="VIS74_01215"
                     /inference="COORDINATES: protein motif:HMM:NF019998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HEY5791886.1"
                     /translation="FVASFVGKANFIPGILRSVEGSRCQVETSDGIIEGFVITSELKP
                     GSKVRVMIRPENLRLSPGSADSRHALIKQVSYLGHGTEYLLAAGDRLFRALELRRRGS
                     IPLAEGTPVSYGWNWDEALVYAEEAAI"
     gene            complement(423..1628)
                     /locus_tag="VIS74_01220"
     CDS             complement(423..1628)
                     /locus_tag="VIS74_01220"
                     /inference="COORDINATES: protein motif:HMM:NF017011.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HEY5791887.1"
                     /translation="MIEGGLWCFEGKRILLLQGPVGPFFQRLSWDLRKAGATVFKLNF
                     NGGDWLFYPTDATPFQGTPEIWPEYFERYLLIKRIELVMLFGDCRPLHRVAHEICLKH
                     RVEVGVFEEGYVRPDFVSFERFGVNDNSLLPRNPRFYLKRKVVRYQLPLKVGNAFWYA
                     VTWAMIYNTASTLLRGAFPHYQHHRTLTVTEGLLWLRSLWRKIYYSSTERGIQEKLLD
                     KQGPRYFLVPLQVHNDAQIQQHSAFEDVEDFIVDVMGSFAKHADPKVVLVFKHHPLDR
                     GCRDYTALITHAAQVLGLEGRVYYIHDQHLPTLIKNALGVVVINSTVGLSSIGHRVPV
                     KTCGQALYNIEGMVYPGSLDEFWKNPVRGDHKLYRKFRNFLVRTTQLNGNFYRRLPLT
                     NSESGIQWM"
     gene            complement(1635..>2162)
                     /locus_tag="VIS74_01225"
     CDS             complement(1635..>2162)
                     /locus_tag="VIS74_01225"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEY5791888.1"
                     /translation="SPSIQERMRREIQDAVGEDEFEFGDAQKLRLTLNVFRETLRLYP
                     PVGFFIREATEDHRIRDKEVGRGCPIMISPWLMHRHRKLWSQPDEFNPDRFETPEGKE
                     SAKCAYLPFSMGPRVCVGQAFATQEAVMVLASIVRRYEILPDPNHVPQTVGRVTVRSD
                     NGIRIRLAKRNPAAD"
BASE COUNT          463 a          663 c          612 g          424 t
ORIGIN      
        1 tttcgtggcg tcattcgtcg gcaaggcgaa tttcattccc ggcatcctgc gatccgtcga
       61 aggctcgcgc tgccaggtgg agaccagtga cgggatcatc gaaggctttg tcatcacctc
      121 cgaactgaaa cccggcagca aggtccgggt catgatccgc cccgaaaacc tgcggctctc
      181 ccccggctcc gccgactcgc gccacgcgct catcaaacag gtcagctatc tcgggcacgg
      241 caccgaatac ctgctggcag ccggtgacag gttattccgc gcactggagc tccgccgccg
      301 cggatcaatc ccgctcgccg aaggcacccc ggtcagctac ggctggaatt gggacgaagc
      361 tctggtttac gccgaagaag cggccatttg acctggaaac tcccgccggt ttggggaaac
      421 ggctacatcc actggatgcc cgactccgag ttcgtcaagg gcaggcgccg gtaaaagttg
      481 ccgtttaatt gggtggtgcg gaccaaaaaa ttccgaaatt tccggtaaag cttgtgatcc
      541 ccgcgaaccg gatttttcca aaattcatcc agcgagcccg ggtaaaccat cccctcaata
      601 ttatacaggg cctggccgca agtcttgacg ggcacgcggt ggccgatcga ggaaagcccc
      661 acggtgctgt tgatgaccac gactcccagg gcgtttttga tcagcgtcgg cagatgctga
      721 tcgtggatgt aatacaccct tccttccagc ccaagcacct gcgccgcgtg cgtgatcaag
      781 gccgtgtaat cccggcaacc ccgatccagc ggatgatgct tgaataccaa aaccaccttg
      841 gggtcggcgt gcttggcgaa ggatcccatg acatcgacaa taaaatcttc gacgtcctcg
      901 aaggcggaat gctgctggat ttgggcgtcg ttatgcacct gcagggggac caggaaatac
      961 cggggaccct gcttatccaa aagcttttcc tggatgccgc gctccgtgga ggaataatag
     1021 atcttccgcc aaagcgatcg gagccagaga agcccttcgg tgaccgttaa ggtgcggtga
     1081 tgctggtaat ggggaaacgc cccgcgcaaa agggtgctcg ccgtattgta aatcatggcc
     1141 caggtcaccg cataccagaa cgcattgccc accttcaacg gcagctggta gcgcacgacc
     1201 ttgcgtttga ggtaaaagcg cggattgcgc ggcaaaagcg aattgtcgtt cacgccgaac
     1261 cgctcgaaac tgacaaaatc gggccgcaca tatccctcct cgaatacccc cacctccacc
     1321 cgatgcttca ggcagatctc atgagcgacg cggtgcagcg ggcggcagtc cccgaacagc
     1381 atcaccagct cgattcgctt gatcagaagg taacgctcga agtattcggg ccaaatctcc
     1441 ggcgtcccct ggaaaggcgt ggcatccgtg ggataaaaca gccagtcgcc tccattgaaa
     1501 tttaatttaa agacggtcgc gcccgctttc cgcagatccc agcttaaccg ttgaaagaac
     1561 ggaccgaccg gcccctgcaa gagcaggatc cgttttccct cgaagcacca gagtccccct
     1621 tcgatcatgc aatcttaatc ggctgccggg ttgcgtttgg ccagccgaat ccggattccg
     1681 ttgtcggaac gcaccgtgac ccggcccacc gtttgcggca cgtgattggg gtccgggagg
     1741 atttcatacc gccggacgat gctcgcgagc accatcaccg cctcctgcgt ggcgaatgcc
     1801 tgcccgacgc agactctcgg ccccatgctg aaaggcaggt aggcgcactt ggccgattcc
     1861 ttgccctccg gagtttcaaa acggtccgga ttaaattcgt cgggctggct ccaaagcttg
     1921 cggtgccggt gcatgagcca gggggaaatc atgatcgggc atccgcggcc gacttccttg
     1981 tcgcgaatcc ggtgatcctc ggtggcctcc cggatgaaaa agccaaccgg gggataaagc
     2041 cggagggttt cccggaagac attcaaggtg agccgcagtt tttgggcgtc gccaaattcg
     2101 aattcgtcct cccccacggc gtcctgaatt tcccggcgca tgcgctcctg aatcgaaggg
     2161 ct
//
LOCUS       DATDAY010000062         3609 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_68854, whole genome shotgun sequence.
ACCESSION   DATDAY010000062 DATDAY010000000
VERSION     DATDAY010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 3609)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3609)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3609
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_68854"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..571
                     /locus_tag="VIS74_01230"
     CDS             <1..571
                     /locus_tag="VIS74_01230"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY5791889.1"
                     /translation="AFLMIVKSALENIGYVGGPRGMLGIRPLTTLPWAFAWASLTIWI
                     LRNLVYSKFGRAIVAIRGDEIAANAMGVRTREAKILAFVVSSFFGGVAGALFAHQILF
                     INPSSFDILKSTEILVMVYLGGIGSLAGSVLGATTFTLLSQWLQPLGTWRMVVMPLML
                     VFLMLFRPRGIMGLKELAWFIPKRELPKK"
     gene            568..1344
                     /locus_tag="VIS74_01235"
     CDS             568..1344
                     /locus_tag="VIS74_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006983916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY5791890.1"
                     /translation="MSELLEISGVTQRFGGLRAISDFNLAVKPGETIGVIGPNGAGKT
                     TLFNLITGIFRPTEGTIRCRGKLLNDRLPNQIAALGIARTFQNIRLFRELTVLDNVRI
                     AYDSQLRYSPLAALLTAPGQRRAEEESIRASLELLGLFQLAGLAGEDAGNLSYGSQRK
                     LEIARALALKPSVLLLDEPAAGMNPSETRELTEFLRWVKSHFAVTILLIEHHMHLVMS
                     LCQRIKVLDFGVTIAEGAPEEIRANPRVIEAYLGEKPEDA"
     gene            1353..2054
                     /locus_tag="VIS74_01240"
     CDS             1353..2054
                     /locus_tag="VIS74_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013801934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY5791891.1"
                     /translation="MLQVENLGVSYGDIHAVKEISFEVNPGEIVALIGSNGAGKTSTL
                     RAISGMTGFAGRVRFAGNDLRGTRADRIVANGLAHVPEGRGIFGTLTVMENLRLGAWI
                     RRDASAVALDYERVMNLFPRLRERREQLAGTLSGGEQQMLAVGRALMSRAKMLLLDEP
                     SMGLAPKLVQEIFSIIAEINREGVSILLVEQNAHMALQLANRACLLETGRVALSGDAK
                     SLLADPAIKKAYLGA"
     gene            2643..>3609
                     /locus_tag="VIS74_01245"
     rRNA            2643..>3609
                     /locus_tag="VIS74_01245"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          757 a          946 c         1134 g          772 t
ORIGIN      
        1 ggcgtttttg atgatcgtga aatccgcgct ggaaaacatc ggttacgtcg gcggcccgcg
       61 gggaatgctc ggcatccgcc cgctgacgac gctgccctgg gcgttcgcgt gggcgtcgct
      121 gacgatctgg attctgcgga atctggttta ttccaagttt ggccgcgcga ttgtggccat
      181 tcgcggcgat gagattgcgg cgaatgcgat gggcgtccgg acgcgcgagg ccaagatcct
      241 cgccttcgtc gtctcctcct tcttcggcgg cgtggcgggc gcgcttttcg cgcaccagat
      301 tttgttcatc aatccttcct cgttcgacat cctgaagtcc accgagattc tcgtgatggt
      361 gtatctcggc ggcatcggct cgctggccgg ctccgtcctg ggggccacga ccttcacgct
      421 gctctcgcaa tggctccagc cgctcggcac ctggcggatg gtggtcatgc cattgatgct
      481 ggtcttctta atgctgtttc gcccgcgcgg aatcatggga ctcaaggaac tggcctggtt
      541 catcccgaaa cgggaactgc cgaaaaaatg agcgagcttc tcgaaatctc gggcgtgacg
      601 cagcgatttg gcggattgcg cgccatttcg gacttcaacc ttgcagtgaa gcccggcgag
      661 acgatcggag tcatcgggcc gaacggggcg gggaaaacga cgctgtttaa tctcattacc
      721 ggcatcttca ggccgacgga gggaacgatc cggtgcaggg gcaagctgct gaatgaccgg
      781 ctgccgaacc agatcgcggc gctgggcatt gcccggacct ttcagaatat ccgcctcttt
      841 cgggaattga cggtgctcga caacgtccgc atcgcctacg attcgcaatt gcgctattcg
      901 cccctggcgg ccctgctgac ggcgccggga cagcgccggg cggaggagga atccatccgg
      961 gccagcctgg aattgctcgg tttgttccaa ctggctggcc tcgccggcga ggatgccggg
     1021 aatctttcct atggctcgca gcggaagctc gaaatcgccc gggcgctggc cttaaagccg
     1081 agcgtgctgc tgctcgacga gcccgcggcg ggcatgaacc cctcggaaac gcgcgagctc
     1141 acggagtttt tgcgatgggt gaaatcgcat tttgcggtga ccattttgtt gatcgagcat
     1201 cacatgcacc tggtgatgag cctgtgccag cggatcaagg tgcttgattt cggggtgacg
     1261 attgccgagg gcgcgccgga ggaaatccgc gcgaacccgc gggtcattga agcttacctg
     1321 ggcgaaaagc cggaggacgc atgagcgcgc ccatcctcca ggtcgaaaat ctcggcgttt
     1381 cctacgggga tattcacgcc gtgaaggaaa tttccttcga ggtgaatccc ggtgagatcg
     1441 tcgcgttgat tggttccaat ggcgccggca aaacttccac cctccgggcg atttccggaa
     1501 tgaccggctt tgccggccgc gtgagatttg cgggcaacga tttgcggggg acgcgggcgg
     1561 atcggatcgt ggcgaacggg ctggctcatg tgcccgaagg ccgcgggatt ttcggcacgc
     1621 tgaccgtcat ggaaaatctc cggctcggcg cctggattcg ccgcgatgcc tccgccgtgg
     1681 cgctggacta cgaacgcgtc atgaatttgt ttccccggct gcgcgagcgc cgcgagcagc
     1741 ttgcaggcac gctttccggc ggcgaacagc aaatgctcgc cgtcggccgc gccctgatga
     1801 gccgggcgaa aatgctgctg ctcgacgaac cctccatggg cctcgcgccg aagctcgtgc
     1861 aggagatttt ctccatcatc gcggagatca accgcgaagg cgtcagcatc ctcctcgtgg
     1921 aacagaacgc ccacatggcc ctgcagctcg ccaatcgcgc ctgcctgctg gagaccggaa
     1981 gagtggcgct gagcggggat gcgaagtcat tgctggccga tccagccatc aaaaaagcgt
     2041 atttgggcgc ctgatttcgg gatttcggcg gcgccgaacc cgaaaatcga aaaatatttt
     2101 gccccgttct tcggggaatt tttttgaatg attggccgtt ttgttttgtg acaaaccgct
     2161 ctgaggccgc ttaaatgctc gattcatggg cggatgggaa atgaaggcgt tgtcaacggc
     2221 tttttccggg cggatggccg ctcgaagaaa gtgaaaatat ttttagaaaa accgttgacc
     2281 cattttggaa tccgtatata cctattcatc ccgcaagcga tcagggttct gagagcaaag
     2341 cggaaatgat cttccaggcg agcttcagct gcctgattga cgagaggctt taacaaggct
     2401 ccttcgtcgt caggagaaac ggcgggacgc catcgagaga gttatttgac agtccggtct
     2461 tggttgaatt cgcagcaatg cgtgaaacca aggtgttttt aagcaaaaac tgaattgtgc
     2521 gtgccttttt taaggtttcg cgatttttaa tttatacggc catcgaacga agtctccgtg
     2581 attcacggag atgatttagt ttgatatgcc agaaatttct cacctcacgg tgagagcaac
     2641 tttctacgga gagtttgatt ctggctcaga acgaacgctg gcggcgtgga taagacatgc
     2701 aagtcgaacg ggactttggg gcttgctcca aggttcagtg gcacacgggt gcgtaacacg
     2761 tgagcaacct gcccggaaat gggggatagc tcgccgaaag gcgaattaat accgcatgtg
     2821 gctaggaggg acatccttcc gaagctaaag tcgtaagacg tttccggatg ggctcgcggc
     2881 ctatcagcta gttggtgagg taacggctca ccaaggcaat gacgggtagc tggtctgaga
     2941 ggacgaccag ccacactgga actgagacac ggtccagaca cctacgggtg gcagcagtcg
     3001 agaatttttc acaatgggcg aaagcctgat ggagcgacgc cgcgtggagg atgaaggcct
     3061 tttggtcgta aactcctgtc atgcgagaac aagaaagtga tagtatcgca agaggaagag
     3121 acggctaact ctgtgccagc agccgcggta atacagaggt ctcaagcgtt gttcggattc
     3181 attgggcgta aagggtgcgt aggcggcgaa gtaagtcgga tgtgaaatcc cggggctcaa
     3241 ccccggaact gcattcgata ctggtttgct cgaggactgg agaggagatt ggaattcatg
     3301 gtgtagcagt gaaatgcgta gagatcatga ggaagaccag tggcgaaggc gggtctctgg
     3361 acagttcctg acactgaggc acgaaggcca ggggagcaaa cgggattaga taccccggta
     3421 gtcctggcag taaacggtgc acgtttggtg tgggcaggtt cagaccctgt ccgtgccgga
     3481 gctaacgcgt taaacgtgcc gcctgggaag tacggtcgca agattaaaac tcaaagaaat
     3541 tgacgggggc ccgcacaagc ggtggagtat gtggcttaat tcgatgcaac gcgaagaacc
     3601 ttacctggc
//
LOCUS       DATDAY010000063         2057 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_2504337, whole genome shotgun sequence.
ACCESSION   DATDAY010000063 DATDAY010000000
VERSION     DATDAY010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2057)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2057
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2504337"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1036)
                     /locus_tag="VIS74_01250"
     CDS             complement(<1..1036)
                     /locus_tag="VIS74_01250"
                     /inference="COORDINATES: protein motif:HMM:NF013106.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HEY5791892.1"
                     /translation="MFTTRRDRTENGYPRQKTLHKLFRAGLTAALVAALVFGTAGLIT
                     FEYFRNKYQTIADSFNLKQVERMEAASIIYDRAGKQFGKIFIQNRNPVAYDQFSPLLV
                     DAVVAAEDSSFYSHKGVDWQGVLRAAITNYTSGRIAQGASTVTQQLARNSFNMRERTF
                     ERKFIEMLLAMRIEKAYSKNQIMEMYLNRVYFGSGFYGAEAAARGYFGKPAKDVNLGE
                     AAMLAGLLRSPQAFSPWNNLEAATAVRNRVLLRMRETGKITADQYRMETESRLYVRKR
                     TNPHKVSYAVDYIRQQVIAALGFERAANGGFHIYTTLDLNIQRTAESSLRAQLDQIER
                     RPDYKHETYAD"
     gene            1170..1745
                     /locus_tag="VIS74_01255"
     CDS             1170..1745
                     /locus_tag="VIS74_01255"
                     /inference="COORDINATES: protein motif:HMM:NF016819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HupE/UreJ family protein"
                     /protein_id="HEY5791893.1"
                     /translation="MRKFSFASLLLMLCLPAAQAHPGHDGGMSLFQGFGHPLTGLDHL
                     LAMVAAGLWAAQIGGRALWVIPVSFVLAMLGGGIAGMNGLALPFLEPGIALSVLLLGL
                     AIAFAFRPPLCVPAILVAAFAIFHGMAHGSEMPATAFGFTFGLGFLLATALLHVAGIA
                     LGLALKKVSPAPLIPALGVGVALLGGYLLIG"
     gene            1859..>2057
                     /locus_tag="VIS74_01260"
     CDS             1859..>2057
                     /locus_tag="VIS74_01260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5791894.1"
                     /translation="MKLFPVTALTAALTFGAYAQEPDPANPNLSTVSNEDSTAKSFWE
                     WDYFTGDWGGLRTTLSDKGLDF"
BASE COUNT          342 a          674 c          577 g          464 t
ORIGIN      
        1 aatccgcata cgtctcgtgc ttgtaatcgg gccgccgctc gatctggtcc agttgggctc
       61 gcagcgagga ctccgccgtc cgctgaatat tcagatccag cgtggtgtaa atgtgaaatc
      121 cgccattcgc ggcgcgctcg aatcccaggg ccgcgatcac ctgctggcgg atgtaatcca
      181 cggcgtagga caccttgtgg ggattcgtcc gcttgcgcac ataaagccgg gattcggttt
      241 ccatgcggta ttgatccgcg gtgatttttc ccgtctcccg catgcgcagc agcacgcggt
      301 tgcgcaccgc ggtggccgcc tcgagattat tccacgggga aaaggcctgg gggctgcgca
      361 gcaagccggc cagcatcgcc gcctcgccga ggttcacgtc ctttgccggc ttgccgaaat
      421 aaccgcgcgc cgccgcttcg gcgccgtaga agccgctgcc gaaatacacg cggttcagat
      481 acatttccat gatctgattc ttcgagtagg ccttctcgat ccgcatcgcc aggagcatct
      541 cgatgaattt ccgctcgaag gttctttcgc gcatgttgaa ggaattccgc gcgagctgct
      601 gcgtcaccgt gcttgcgccc tgggcgatgc gcccgctggt gtagttcgta atcgccgccc
      661 ggagcacccc ctgccagtcc acgcccttgt gggaataaaa gctgctgtcc tccgcggcca
      721 ccaccgcatc gacgagcagc ggcgagaatt gatcgtaggc caccgggttg cggttctgga
      781 tgaagatttt tccaaactgc ttgcctgcgc ggtcgtaaat gatcgaggcc gcctccatgc
      841 gctccacctg cttcaggtta aagctgtcgg cgatcgtctg atatttattc cggaaatact
      901 caaaggtgat gagccccgcc gtgccaaaga caagcgcggc gaccagcgcg gcggtgaggc
      961 cagcccggaa gagtttgtga agcgtcttct gccgtgggta gccgttttcc gtgcgatcac
     1021 ggcgggtggt aaacatggat ctcaagttct cgaaggatgt ccgaatccct actatggcaa
     1081 atttcccgcc cgtggcaatg gcccagtcaa tcgccgcccg cgactttgca gttgagtccc
     1141 gcggccaagt ggtatcaata taccgttcca tgagaaaatt ttccttcgcc tcgttgctcc
     1201 tcatgctttg cttgccggcc gctcaggcgc atcccggaca cgatggcggg atgagccttt
     1261 ttcagggttt cgggcacccg ctcaccggcc tggaccacct gctcgccatg gtcgccgccg
     1321 ggctctgggc cgcgcaaatc ggcggccgcg ccctctgggt gatccccgtc tcctttgttc
     1381 tcgccatgct cggcggcggc atcgccggca tgaacggcct cgcccttccc ttcctggaac
     1441 cgggcatcgc cctctccgtg ctgctgctcg ggctggccat cgccttcgcc ttccgcccgc
     1501 cgctttgcgt gcccgcgatc ctcgtcgcgg ccttcgcgat tttccacggc atggcgcatg
     1561 gctcggaaat gccggcaacg gccttcggat tcacctttgg cctgggcttc ctgctggcca
     1621 ccgcgctgct gcatgtggcc ggcatcgcgc tcggcctggc attaaaaaag gtctctcccg
     1681 ccccgctcat tccggcgctg ggagtaggcg tggccttgct cggcggctat ctgctcatcg
     1741 gctgaaaatc tccccggccg cagataatcc gccggaatcg acgcgcaatt caccgtcaat
     1801 ttttcgcaaa ctatttttct ttacgacgtg atactgagtc tcaattatca caagccgtat
     1861 gaagcttttc ccagttactg ccttgactgc ggcgctcacc tttggcgctt acgcccagga
     1921 acctgatccc gccaatccaa acctttccac cgtttccaac gaggattcca ccgccaaatc
     1981 tttttgggaa tgggactact tcaccggcga ctggggcggt ctccgcacca cgctctccga
     2041 caagggcctt gattttg
//
LOCUS       DATDAY010000064         5171 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_106865, whole genome shotgun sequence.
ACCESSION   DATDAY010000064 DATDAY010000000
VERSION     DATDAY010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 5171)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5171)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5171
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_106865"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..400)
                     /locus_tag="VIS74_01265"
     CDS             complement(<1..400)
                     /locus_tag="VIS74_01265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5791895.1"
                     /translation="MIPSFHPIPSALRSLACGVFSFSLLGAFGEPVRAQEGRAEEAVS
                     RLDPVMVEADYDTQVTGPFLPDIEGTRINVGKKTSNIRLQELPEISNGNYRQVIAKTP
                     GLILAEESTPLVSIGYRGFDPHRMQYMQVLE"
     gene            504..1139
                     /locus_tag="VIS74_01270"
     CDS             504..1139
                     /locus_tag="VIS74_01270"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HEY5791896.1"
                     /translation="MNEPALIEYFIKGGPIMWPLLLTSLVALTIVIERLIFIARERLR
                     RAPETVAAILGAVEHGDADRAVQIGRGSPDFVARTLTYGLAHRDKSLSDALLRAASRE
                     LARFNRGISILDTIVTLAPLLGLLGTVTGMIRSFGLLGADELGAPAAITGGIAEALIA
                     TAFGLGIAIVALIPFNYLNARLEEARLQIQDAASHVELHLARPPAPASLLS"
     gene            1136..1546
                     /locus_tag="VIS74_01275"
     CDS             1136..1546
                     /locus_tag="VIS74_01275"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEY5791897.1"
                     /translation="MNIPSPRANRKARIEIVPLIDIIFFLLATFVMVSLSMVKNQGIP
                     VNLPAAATSEPQERADPATVTLTAGGTLFFNKEAVTREQLSARLQALKASDPDPRVFI
                     NGDARAEFGLAVAVLDEARRLGILKVAIETTAAR"
     gene            1543..2226
                     /locus_tag="VIS74_01280"
     CDS             1543..2226
                     /locus_tag="VIS74_01280"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HEY5791898.1"
                     /translation="MNPDRPGRWYLAAFLLAAGAHAALFTALPARPAPAAGKAAPVEV
                     ELVAARPLETLPPEPPSAPPPEPAEIPPEPEPAPEPPAEPPPAPAATPEMTLPKPAPA
                     PKAPAPPKNSVPSRKAAPPAAPVKPPASGSSGAVREARPDTPRNRPPHYPEIARRNGW
                     EGVVIVRARVTAAGRVSSVSIHRGSSYGVLNQAALAAVKSWRFRPGSLGGQAVESAVE
                     VPVNFSLRR"
     gene            complement(2251..4737)
                     /locus_tag="VIS74_01285"
     CDS             complement(2251..4737)
                     /locus_tag="VIS74_01285"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF006032.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HEY5791899.1"
                     /translation="MSKTLVIAEKPSVATDLARALGKIPKKDDYFENDEFVISSAVGH
                     LVELCLPGEMDKKRGKWSFANLPIIPDHFDLKPIEKNESRFKLLKKLLKRADVTTVIN
                     ACDAGREGELIFHYIMQLTGSQKPAQRLWLQSMTPEAIRSAFAELRSAQEMAALGDAA
                     VCRSESDWLVGINGTRAMTAFNSKGGGFQLTPVGRVQTPTLAILAEREEKIREFKPRT
                     YFEVFGDFAVIGGGYRGRWFDEAFKKGGEEDARAERLWDKAKAEAILAKCLGQPGAIE
                     EEKKPASQIPPLLYDLTTLQREANSRFSFSARRTLQLAQALYERHKVLTYPRTDSRYL
                     PEDNLGNVKAALTKFEDAELAGHAAKALAKGWVGLNKRVFNNTKVTDHHAIIPTGVAP
                     KNLDEAEQKLYDMVARRFIAVFYPAAQFEITIRVTRVAGEAFKSDGKIIIDPGWLAVY
                     GKQAAVEDDQILTPVQSGETAQTTNVEIKESQTKPPARFNEATLLSAMEGAGKLVDDE
                     ELREAMSQRGLGTPATRAQIIEGLIFDGYLVRQGRDLIATSKGMSLITLLRGIGVEAL
                     TSPEMTGEWEFKLKRMEQGQMPRPQFMDEIRKFTEEIVLKAKNFSGDAVEGRFLDLEA
                     KCPRCGGGVFKESFKAYECPECKLLIWKNMAGRELEREEVKTLLEAGQVGPLEGFRSK
                     MGRPFSAIVKLGEEFKPTFDFGNDKTETQEIDFSQLPPIGPCPVCQKGTIFNAEKAYV
                     CDHTADKSCTFRMGKTILQREIPLEQVQKLLQTGKTDLIPRFISKKGKPFSAFLKLEK
                     GAVGFEFEPRKPAAPKKRSAANASTQAEAA"
     gene            complement(4745..>5171)
                     /locus_tag="VIS74_01290"
     CDS             complement(4745..>5171)
                     /locus_tag="VIS74_01290"
                     /inference="COORDINATES: protein motif:HMM:NF036806.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HEY5791900.1"
                     /translation="SFGTLVVEAGLNSGALITANQAAEQGRSLYVVPGRIDHPSAFGS
                     NRLIQQGAKLVMSAQDILDDLGMLFSKVPDLPAHTPAVELSGEERRIYEALGNDERTV
                     DEIVAKSGLPSPIVSSTLLGFEMRHLAKARPGGRYVRLI"
BASE COUNT          844 a         1698 c         1615 g         1014 t
ORIGIN      
        1 cttccaaaac ctgcatgtat tgcatgcggt gcgggtcgaa accgcggtag ccgatgctca
       61 ccagcggcgt cgattcctcc gcgaggatga gtccgggcgt cttcgcgatg acctggcggt
      121 agttgccgtt gctgatctcg ggaagctcct ggagccggat gttcgacgtt tttttgccga
      181 cattgatgcg ggtgccctcg atgtcgggca aaaatgggcc ggtcacctgc gtgtcgtaat
      241 cggcttccac catgacggga tcaaggcggg agacggcctc ctcggcgcgg ccctcctgcg
      301 cccggacggg ctcgccgaac gcgccaagca gggaaaatga aaaaactccg caggccaggg
      361 accttaaggc ggatgggatg gggtggaatg agggaatcat gggcggagca atattgagat
      421 tcgatctcat ttgaaaagaa aaaatttgag attaaatctc aagagggctt gaagcgggga
      481 aaaattcgct ggaagatcgc cggatgaacg agcctgcgct catcgaatat tttatcaaag
      541 gcggcccgat catgtggccg ctgctgctca cctcgctcgt tgcgctcacg atcgtcatcg
      601 agcggctgat tttcatcgcg cgcgagcgcc ttcgccgcgc gccggagacc gtggccgcca
      661 ttcttggggc cgtggagcac ggagacgccg accgcgccgt gcagatcggc cggggttcgc
      721 ccgatttcgt ggcccgcacg ctcacctatg gcctcgcgca ccgcgacaag tcgctttccg
      781 acgccctcct gcgcgcggcc agccgggagc tcgcccgctt caaccgcggc attagcattc
      841 tcgacaccat cgtcaccctc gccccgctgc tcggcctgct cggcaccgtc acgggaatga
      901 ttcgctcttt cggcctgctc ggggccgatg aactgggagc gcccgccgcc atcaccggcg
      961 gcattgccga ggcgctcatc gccaccgcgt tcgggctcgg catcgccatc gtggcgctca
     1021 tccccttcaa ctacctcaac gcccgtctcg aagaagcccg gctccagatt caggacgcgg
     1081 cctcccacgt cgagctgcac ctcgcccgcc cgcccgcgcc ggcgtccctg ctttcatgaa
     1141 cattccctcg ccccgcgcca accgcaaagc gcgcatcgag attgtcccgc tcatcgacat
     1201 catctttttt ctgctggcga cgttcgtcat ggtttccctc tccatggtca aaaaccaggg
     1261 catccccgtg aaccttcccg ccgccgccac cagcgaaccg caggaacgcg ccgatccggc
     1321 caccgtcacc ctcacggctg gcggaaccct ttttttcaac aaggaagccg tcacccgcga
     1381 gcagctcagc gcccggctcc aggcgctcaa ggccagcgac cccgatccgc gcgtcttcat
     1441 caacggcgat gcccgggcgg aattcggcct ggcggttgcc gttctcgacg aagcgcgccg
     1501 tctcggcatc ctcaaagtcg ccatcgaaac caccgcggcc cgatgaaccc cgaccggccg
     1561 ggccgctggt atctggccgc tttcctcctc gccgccggcg cgcatgccgc cctcttcacg
     1621 gcgctgcccg cccgcccggc gcccgccgcc ggcaaggccg cgcccgtgga ggtggaactc
     1681 gtcgccgccc gcccgctcga aactctcccg cccgagccgc cgtcggcgcc gccacctgag
     1741 ccggcggaaa ttcccccgga acccgaaccc gctccggaac ccccggccga accgccgccg
     1801 gcgcccgccg ccacgccgga gatgaccctc cccaagcctg ctcccgcgcc caaggccccg
     1861 gctccgccca aaaactcagt tccttcgcga aaagccgcgc cgccggcggc ccccgtaaaa
     1921 ccacccgcca gcggctcctc cggcgccgtg cgcgaagccc ggcccgacac gccgcgcaac
     1981 cgtcctccac attatcccga gatcgcccgg cgcaacggct gggaaggcgt cgtgatcgtg
     2041 cgagcccgcg tcaccgccgc cggacgggtc tcctccgtct ccatccaccg cggttcaagc
     2101 tacggcgtgc tcaaccaggc cgcgctggcc gcggtgaaaa gctggcgctt tcggccgggt
     2161 tccctcggcg ggcaggccgt ggaaagcgcc gtcgaggtgc cggtgaattt ttcgctgcgg
     2221 cgataggccc gcgcgcggtt cgccaagggc tcaagccgcc tcggcttgag tggatgcgtt
     2281 ggccgcggat cgctttttgg gggccgccgg tttgcggggt tcgaattcga agccgaccgc
     2341 gcctttttcc agtttgagga aggccgagaa cggtttgcct tttttggaaa tgaagcgcgg
     2401 gatcaggtcg gtcttgccgg tctgcagcag cttctgcacc tgctccaggg ggatttcccg
     2461 ctgcagaatc gttttgccca tgcggaaggt gcaggatttg tcggcggtgt gatcgcacac
     2521 atacgctttt tcggcgttga agatcgtgcc tttctggcaa accgggcacg ggccaatggg
     2581 tgggagctgc gagaaatcga tttcctgcgt ctcggttttg tcgttgccga aatcgaaggt
     2641 cggcttgaat tcctcgccca atttgacgat ggcggaaaag ggccggccca ttttgctgcg
     2701 aaagccctcg agcggcccca cctggccggc ttcgagcagc gtcttgactt cctcgcgctc
     2761 caattcgcgg ccggccatgt ttttccagat gagcagcttg cactcggggc attcataggc
     2821 cttgaaggat tccttgaaaa ccccgccgcc gcagcgcggg catttcgcct cgagatcgag
     2881 gaagcggcct tcgacggcgt cgccggagaa attttttgcc ttcaggacga tttcctcggt
     2941 gaatttccgg atttcgtcca tgaattgcgg gcgcggcatc tggccttgct ccatccgctt
     3001 gagtttgaac tcccattcgc cggtcatttc cggcgaggtg agggcttcca cgccgatgcc
     3061 gcggagcagc gtgatgagcg acatgccctt ggaagtggcg atcaaatcgc gcccctggcg
     3121 gacgaggtaa ccgtcgaaaa tcaggccttc gatgatctgc gcgcgcgtgg ccggcgtgcc
     3181 caggccgcgc tggctcatgg cctcgcggag ctcctcgtcg tccacgagct tgccggcgcc
     3241 ttccatggcc gacaagagcg tggcttcatt aaagcgggcg ggcggcttgg tttggctttc
     3301 cttgatctcg acgttcgtgg tctgcgccgt ttcgccggac tgaaccggcg tcagaatctg
     3361 gtcgtcctcc accgcggctt gtttgccgta aaccgcgagc cagccgggat cgatgatgat
     3421 tttgccatcg cttttaaacg cctcgcccgc cacgcgggtg acgcggatgg tgatttcaaa
     3481 ctgcgccgcg ggataaaaga cggcgatgaa gcgccgcgcg accatgtcgt aaagtttctg
     3541 ctcggcctcg tccaaatttt tgggcgcgac gccggtcggg atgatcgcgt ggtggtccgt
     3601 gaccttggta ttgttgaaga cgcgcttgtt cagtccaacc cagcccttcg cgagcgcctt
     3661 ggcggcatgg ccggcgagtt cggcatcctc gaatttcgtg agggccgcct tgacgttgcc
     3721 gaggttgtcc tcgggcaggt aacgggaatc ggttcgcgga tacgtcagca ccttgtggcg
     3781 ctcgtaaagc gcctgcgcga gctgcagcgt gcggcgggcg ctgaagctga agcggctgtt
     3841 ggcctcgcgt tgcagcgtgg tcaggtcgta gagcagcggc gggatttgcg aggcgggttt
     3901 tttctcctcc tcgatggcgc cgggctggcc gaggcatttg gcgaggatcg cttcggcttt
     3961 cgccttgtcc cagaggcgct cggcgcgggc gtcctcttcg ccgcccttct tgaaggcctc
     4021 gtcgaaccag cggccgcggt agccgccgcc aatgacggca aaatccccga agacctcgaa
     4081 atacgtgcgc ggcttgaact cccggatttt ttcctcgcgc tcggcgagaa tggccagcgt
     4141 cggggtctgc acgcggccca ccggggtgag ctggaaaccg cctcccttgg aattgaacgc
     4201 ggtcatggcc cgcgtgccat tgatgcccac gagccagtcg ctctcggaac ggcagaccgc
     4261 cgcgtcgccg agcgcggcca tttcctgggc gctgcgcagt tccgcaaacg cggagcggat
     4321 ggcttccggc gtcatggatt gcagccacag gcgctgggcc ggcttttggc tgccggtgag
     4381 ctgcatgatg taatggaaga tcagctcgcc ttcgcgcccg gcgtcgcagg cattgatgac
     4441 ggtcgtgaca tcggcgcgct tgagcagctt tttgagcagc ttgaagcggc tttcgttctt
     4501 ctcgatcggc ttgaggtcga agtgatcggg aatgatcggc aggttcgcga agctccattt
     4561 gccgcgcttc ttgtccattt cccccggcag gcataactcg acgaggtggc cgaccgccga
     4621 ggaaatgacg aattcgtcat tctcaaaata gtcgtccttc ttcgggatct tgccgagcgc
     4681 gcgcgccaga tcggtggcga cgctgggttt ttcggcgatg acgagggttt tagacatggc
     4741 ggttttaaat gagtcgaacg taacggccgc cgggccgggc tttcgccagg tggcgcattt
     4801 caaagccgag caacgtagaa gacacaatcg gcgatggcaa acccgatttt gcaactattt
     4861 catccacagt gcgttcatca tttcccagcg cctcgtatat ccgccgttct tcgccggaaa
     4921 gctccacggc gggcgtgtgc gcgggcagat cgggaacctt ggaaaagagc attcctaggt
     4981 cgtcgagaat gtcctgcgcg ctcatgacca gcttcgcgcc ttgctgaatt aagcggttcg
     5041 aaccgaacgc gctgggatgg tcgatccgtc ccggcacgac gtagagcgac cgcccctgct
     5101 cggcggcctg gttggccgtg atgagggcgc cgctgttgag cccggcttcc acgacgaggg
     5161 tgccgaagga c
//
LOCUS       DATDAY010000065         2213 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_467376, whole genome shotgun sequence.
ACCESSION   DATDAY010000065 DATDAY010000000
VERSION     DATDAY010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2213)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2213)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2213
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_467376"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..292)
                     /locus_tag="VIS74_01295"
     CDS             complement(<1..292)
                     /locus_tag="VIS74_01295"
                     /inference="COORDINATES: protein motif:HMM:NF012314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEY5791901.1"
                     /translation="MKTLLACSLLILATLPVFAGENAWFDNYDTALAQAKKENKPVLV
                     DFTGSDWCPPCKMMDKEVFASTDFQTYAAKSLVLLRVDFPKLKPLSAELQKRN"
     gene            complement(327..509)
                     /locus_tag="VIS74_01300"
     CDS             complement(327..509)
                     /locus_tag="VIS74_01300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5791902.1"
                     /translation="MSKTLCDWSKSDIEKHFDKLCELVQEPRFVCRKCARSARSSKSL
                     CKPQAIGKSPHAPHSH"
     gene            588..1061
                     /locus_tag="VIS74_01305"
     CDS             588..1061
                     /locus_tag="VIS74_01305"
                     /inference="COORDINATES: protein motif:HMM:NF012436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HEY5791903.1"
                     /translation="MKTQAASAHKTNAVHEGLKTVLADSYALMGQTHLAHWNVEGPNF
                     FQLHTVFQSQYEELFSAIDDIAERLRALDVLAPGGLKTLAKLSEISELGVETMPAKDF
                     VAHLIESHELLVAHAHALREAAGEADDLETQDLAIGRLQVHQKTLWMLKSFLKNL"
     gene            complement(1149..>2213)
                     /locus_tag="VIS74_01310"
     CDS             complement(1149..>2213)
                     /locus_tag="VIS74_01310"
                     /inference="COORDINATES: protein motif:HMM:NF012457.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HEY5791904.1"
                     /translation="GVSHYRFGVEWARVEPQPGRYNEAAIRRYVEMARKLKAAGIEPI
                     VTLWHFTFPSWMVTPKKPGKSRWLHPLYRERWPAYVEKMVKAFGPAVRVYAPQNEANG
                     DLALGYISATWPPGLFLDFGKHAAALRESVWGFRRAAEIIRENRKDALVISVTALPYW
                     IQTLWDPTRAYYNFMQRINFAYLDRIYDVCDLIGINYYYTEYADFTALVALKARRGRN
                     YSLLGWVIQPHSLYKQIRTISKRYQKPVLITENGIATSKDIKRVRYLFNHMLEVKEAI
                     HDGYDVRGYFVWTLVDNYEWHHGYKAPFGMTRMDPLTKQRLLRPSALFYKGIIEVSNA
                     SSMLDEVSSKPELPPGFIER"
BASE COUNT          449 a          627 c          676 g          461 t
ORIGIN      
        1 cattgcgttt ttgcaactcc gcgctcagcg gcttcagctt gggaaaatcg acccggagca
       61 gcacgaggga cttggcggcg taggtctgaa aatcggtgga ggcaaaaacc tctttgtcca
      121 tcatcttgca gggagggcac caatccgatc cggtgaaatc aaccagcacc ggcttgtttt
      181 cctttttagc ctgggccagg gcggtatcgt aattatcaaa ccaggcattt tccccggcaa
      241 aaacgggcag ggtggcgaga atgagcagcg agcaggcgag gagagttttc atattgctaa
      301 attggacaac cgcggatggg aaaagttcaa tgcgaatgcg gcgcgtgcgg ggattttccg
      361 atggcctggg gcttgcataa cgatttcgaa ctccgggcgg aacgggcgca cttgcggcac
      421 acaaatcgcg gttcctgaac cagttcacac aatttgtcga aatgtttctc gatgtccgac
      481 ttggaccagt cgcagagggt tttggacatc catttcacca aaccgcagct tcgcgggttg
      541 cgcaagcggc aactttctgc tatttagaat aaaactagat aaagattatg aaaacccaag
      601 ccgcttccgc tcacaagacc aatgccgttc acgaaggact caagaccgtg ctggccgatt
      661 cttatgccct catggggcag acgcacctcg ctcattggaa cgtggaaggg ccgaactttt
      721 tccagctcca caccgtgttt cagtcgcaat acgaggagct tttttcggcc attgacgaca
      781 tcgccgaacg cctccgcgcg ctggacgtgc tcgcgccggg cgggctgaag accctcgcca
      841 agctttcgga aatttccgaa ctcggggtcg agacgatgcc ggccaaggac ttcgtggccc
      901 acttgatcga atcccatgaa ttgctggtgg cccatgccca cgccctccgc gaagcggccg
      961 gggaggcgga cgatctggaa acgcaggatc tggccatcgg ccgcctgcag gtccaccaga
     1021 aaaccctttg gatgctgaag agcttcctga agaatctgta acccgcttgg ttctaaaagg
     1081 caacggacgg ctcggcgagc tgtccctgcc ctttttaggt agggcgggtt cgatgaaccc
     1141 gccgcctttt accgctcgat gaaccccggc ggcagctccg gcttgctcga cacctcgtcg
     1201 agcatggacg aggcgttcga gacttcgatg atgcctttgt aaaagagcgc ggagggccgc
     1261 agcaggcgct gcttggtcag cggatccatg cgcgtcatgc cgaagggggc cttgtagcca
     1321 tggtgccatt cgtaattgtc cacgagcgtc cagacgaaat aaccgcgcac gtcgtagccg
     1381 tcatggatgg cttccttgac ctccagcatg tggttgaaga gataacgcac gcgcttgatg
     1441 tccttcgagg tggcgatgcc gttttcggtg atgagaaccg gtttctggta acgcttgctg
     1501 attgtccgga tctgcttgta gagcgagtgc ggctggatga cccagcccag caggctgtag
     1561 ttccggccgc gccgggcttt gagggcgacc aacgccgtga agtcggcgta ttcggtgtag
     1621 taataattga tgccgatcag gtcgcagaca tcgtaaatgc ggtccagata cgcgaaattg
     1681 atccgctgca tgaaattata atacgcccgg gtcggatccc acagcgtctg aatccaatac
     1741 ggcagggcgg tgacgctgat gacgagcgca tccttgcggt tctcgcgaat gatttccgcc
     1801 gcccggcgga atccccagac gctttcccgc agcgcggcgg cgtgcttgcc gaagtcgagg
     1861 aagagtcccg gcggccaggt cgcgctgatg tagccgagcg cgaggtcgcc attggcctcg
     1921 ttctgcggcg catacacgcg cacggccggc ccgaaggctt tcaccatttt ttcgacgtaa
     1981 gccggccagc gttcgcggta gagcgggtgc agccagcgcg acttgccggg cttcttgggg
     2041 gtgaccatcc agctggggaa ggtgaagtgc cacaaggtta cgatgggctc gatgcccgcc
     2101 gccttcagtt tccgcgccat ttccacgtag cggcggatgg cggcctcgtt gtagcgcccg
     2161 ggctggggtt cgacgcgggc ccattccacg ccgaagcggt aatggctgac gcc
//
LOCUS       DATDAY010000066         3746 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_1649111, whole genome shotgun sequence.
ACCESSION   DATDAY010000066 DATDAY010000000
VERSION     DATDAY010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 3746)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3746)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3746
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1649111"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..825)
                     /locus_tag="VIS74_01315"
     CDS             complement(<1..825)
                     /locus_tag="VIS74_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY5791905.1"
                     /translation="MENVIIVGTGCAGLTAAIYTARANLSPLVMEGRQPGGQLTTTTA
                     VENFPGFPEGVDGPELIMRMHQQAEKFGARFEYGVVEEFEQANGINRLKVDGNWLETR
                     ALIVASGASARWLGIDREKELIGHGLTSCATCDGAFYRNVPVCVVGGGDSACEEAGFL
                     TRFASKVYLIHRRNELRASKIMADRALANPKIEPIWDSAPVEYLTDAEGEMRGVKLKN
                     LKTGELGELEVKCVFVAIGHEPNTAPFRGKLDMDANGYLIQTSGTHTNLPGVFAAGD"
     gene            912..2240
                     /locus_tag="VIS74_01320"
     CDS             912..2240
                     /locus_tag="VIS74_01320"
                     /inference="COORDINATES: protein motif:HMM:NF016830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HEY5791906.1"
                     /translation="MPFRIALPPLLLAVLLALPARADYLPRNIYLYDVSLEEFPITAL
                     TRMEDSVESLWTQSGNAGFDPFLYYWGDFLASPVGGKTPAASWMQLLIFGGEFDLEKI
                     AGWSGGSLFVSAVDAAGSNLSLDIGNLFTVSQAYVMNTFSLYDLYFKQRWLDDKLEFR
                     IGRMSAGQYFATLPAMGLVVSGAVNGNPTSLFVNAPFHSTASASWAAYAKFRPLDQFY
                     FDAGIFQASPRTGNPAYHGADFSIRSDDGILLMSEAGWTPVFGAEPKAGSAGKKSASA
                     NPGLPGIYTFGGYYSRYTFEKFAGGTERNACGFYGLAQQMIWRNPLNENNNLSLWAGL
                     TYSPQQSFALMPLMGMGGVIWQGFIPKRDSDSTLFSVYSGGFSRDYSATQVSAGAGRA
                     TAETVLEWSYIIQLTKNVQLQPDLQYIIRPSGLGSIPNALVVGFQVSALF"
     gene            complement(2344..2775)
                     /locus_tag="VIS74_01325"
     CDS             complement(2344..2775)
                     /locus_tag="VIS74_01325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5791907.1"
                     /translation="MTVFLDSRVLLSAAGSEKSLSRLIVVLARKKGWRLITAAYCRAE
                     TNRNVFKFGSEAVERWEAMKTGLVFVPDALTSQRPLLLAAAKDKPVLISALAAQSQAL
                     LTLDRNDFGIVLETEVYGMWVTTPRGFLLREGEDEESAIRG"
     gene            complement(2772..2918)
                     /locus_tag="VIS74_01330"
     CDS             complement(2772..2918)
                     /locus_tag="VIS74_01330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5791908.1"
                     /translation="MMLAELRDGGVFLQPAAAMPVRDIPLEQMEQWIAEDEEGAEAFW
                     DKAR"
     gene            complement(3203..>3746)
                     /gene="thiE"
                     /locus_tag="VIS74_01335"
     CDS             complement(3203..>3746)
                     /gene="thiE"
                     /locus_tag="VIS74_01335"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEY5791909.1"
                     /translation="ARMLDGGVGMLQLRAKQWGEAPIAKLAEKIQPLCARAGVPFILN
                     DFPQLVPVVGADGAHVGQDDLPVAEARALAGGGKIIGKSTHSLAQAEAAWAERPDYIG
                     FGPLFATATKPDYVPIGTEDIAEVHRRMNLPIFCIGGIKLENLRRVVEAGAKRVVIVS
                     GILQAADIPAYCRECLGRLP"
BASE COUNT          711 a         1260 c          990 g          785 t
ORIGIN      
        1 gtcaccggcg gcgaagaccc cggggaggtt cgtgtgggtg ccgctcgtct ggatgaggta
       61 gccattcgcg tccatgtcga gcttgccgcg gaacggagcc gtgttcggtt cgtggccgat
      121 ggcgacaaac acgcacttca cctccagctc gccaagctcg ccggtcttta aatttttcag
      181 cttcacgccg cgcatctcgc cttcggcatc ggtgagatac tcgaccggcg ccgaatccca
      241 gatgggctcg attttcggat tcgccagcgc gcggtcggcc atgattttcg aggcccgcag
      301 ttcattccgg cggtgaatca ggtaaacctt cgaggcaaaa cgcgtgagga aacccgcctc
      361 ctcgcaggcg gaatcgccgc cgccgaccac gcagaccggg acattgcggt aaaaagcccc
      421 atcgcaagtc gcgcaggaag tcaggccgtg gccgatcaat tccttttcgc gatcaatccc
      481 cagccagcgc gccgaggcgc cgctggcgac aatcagggct cgcgtctcca gccagttgcc
      541 gtccaccttg aggcggttga tgccattggc ttgctcgaat tcctccacca cgccgtattc
      601 aaagcgcgcg ccaaattttt ccgcctgctg gtgcatgcgc atgatcagct ccggtccgtc
      661 cacgccctcg gggaagccgg gaaaattttc gaccgccgtg gtcgtcgtca attgtccgcc
      721 gggctggcgg ccctccatga cgagcggact gagattggcc cgggcggtat aaatcgcggc
      781 ggtcaaaccc gcgcagcccg ttcccacaat gatgacgttt tccatagagc cggcaaatta
      841 aacacgaatg gccccacgcg ggaaacaacg aaaataccca cgcggcacaa attctgcttt
      901 gtctgccagc gatgccattc cgcattgctt tgccgccctt gctcctggcc gtccttctgg
      961 cccttcccgc ccgggcggac taccttcccc ggaatatcta tttatatgat gtttcactgg
     1021 aggaatttcc catcacggcg ctgacgcgca tggaagattc cgtcgaatcc ctctggaccc
     1081 aatcgggcaa tgccggcttt gatccctttc tgtattattg gggggatttt ctggccagtc
     1141 ccgtcggggg gaaaaccccg gccgcctcct ggatgcaact cctcatcttc ggcggggagt
     1201 tcgacctcga aaaaatcgcc ggctggtccg gcggctccct cttcgtctcc gcggtggacg
     1261 ccgccggctc caatctctcg ctcgacatcg gcaacctctt taccgtgtcg caagcctacg
     1321 tgatgaacac cttttccctg tatgacctgt atttcaagca gcgctggctc gacgacaagc
     1381 tggaattccg cattggccgc atgtcggcgg ggcaatattt tgccaccttg ccggcgatgg
     1441 ggctcgtcgt cagcggcgcg gtgaatggca atcccacctc gcttttcgtg aacgccccct
     1501 ttcattccac cgccagcgcc tcctgggcgg cctacgccaa gttccggccc ttggatcaat
     1561 tttacttcga cgccggcatc ttccaagcct ccccccgcac cggaaatccg gcttatcacg
     1621 gcgcggattt ttccattcgt tcggacgacg gcattttgct catgagcgaa gcgggctgga
     1681 caccggtttt tggcgcggaa ccgaaggccg gcagcgccgg caaaaaaagc gcctccgcca
     1741 atccggggtt gccgggcatt tacacgtttg gcgggtatta ttcccgctac accttcgaga
     1801 aattcgcggg cggcacggag cggaatgcct gcggatttta tgggctggcg cagcaaatga
     1861 tctggcgcaa tcccctgaat gaaaacaaca acctttccct ctgggcgggg cttacgtatt
     1921 cgccccagca atcgtttgcc ctcatgccgc tcatgggaat gggcggagtc atctggcagg
     1981 gattcattcc caaacgcgac tcggacagca ccctgttcag cgtttattcc ggcgggttca
     2041 gccgcgatta ctccgccacc caggtctccg ccggagccgg ccgggccacc gctgaaaccg
     2101 tgctcgaatg gagctacatc attcagctga cgaaaaacgt gcagctccag cccgatcttc
     2161 aatacatcat ccggcccagc gggcttggca gcatccccaa cgcgctcgtc gtgggtttcc
     2221 aggtctcggc gcttttttaa gcgcgaactt ggagggatcc aaccgcggat tccgcggatg
     2281 ggcgcggata atcgatcttt ttgatttacc atatttccag ggatttatcc gcgaaaattt
     2341 gcgtcagccg cggatagcac tttcttcatc ctccccttcg cgcagcagaa atcctcgtgg
     2401 cgtggtgacc cacattccat acacctcggt ttcgagcacg attccgaagt cattccgatc
     2461 caaggtcagc agcgcctggc tctgcgcggc cagggcggaa atcaggaccg gtttgtcctt
     2521 ggcggcggca agcagcaatg gtcgttgact cgtcagcgca tccggaacaa aaaccaatcc
     2581 ggtcttcatg gcttcccatc gctccaccgc ttcggaacca aatttgaaca cgttccgatt
     2641 ggtttccgcc cggcaataag ccgccgtgat caacctccag cccttttttc gggcaagaac
     2701 cacgatcaac cgcgacagcg atttttcgga tccggcggcg gataacagca cgcggctgtc
     2761 gagaaaaacc gtcacctcgc cttatcccag aatgcttccg cgccctcctc gtcctccgcg
     2821 atccattgct ccatctgctc caacggaata tcccgcaccg gcatcgccgc cgccggctgc
     2881 aaaaaaacac ccccatcccg cagctccgcc aacatcatcg ggtgctcgct tgtctccaat
     2941 ccgccgaaag gcgccgctcc gttccggtga aatcccaaat gggcggcaag agcctgccgc
     3001 cctccactga ccagtctcct cggcgtctcc gcgtctcggc gcctttttga atcatccctc
     3061 acccgctggc attttttgag cgccattttg gaggaatcca accgcggatc acgcggatgg
     3121 gcacggataa tcgatctttt atttaccata tttccaggga tttatccgcg aaaatccgcg
     3181 tgatccgcgg ttaaaaatat cctcacggca accggccgag acactcccgg caatacgccg
     3241 gaatatccgc cgcctgcagg attccggaaa cgatcaccac ccgtttcgcg cccgcctcaa
     3301 ccacccggcg gagattttcc aatttgatgc cgccgatgca gaaaatcggc aaattcatcc
     3361 gccggtgaac ttccgcgatg tcctccgttc cgatcggcac gtaatccggc ttggtcgccg
     3421 tcgcaaacag cgggccgaat ccaatgtagt ccggccgctc agcccacgcg gcttccgcct
     3481 gcgccaaact gtgcgtggat ttgccgatga tttttccccc acccgccagc gcccgcgctt
     3541 ccgccaccgg caaatcgtcc tgtcccacgt gcgccccatc ggccccgacg acgggaacca
     3601 actgcggaaa gtcgttcagg ataaagggaa ctcccgcccg cgcgcacaag ggctggattt
     3661 tctccgccaa tttcgcgatc ggcgcttccc cccattgctt ggcccgcaac tgcagcatcc
     3721 ccacgccgcc gtccagcatc cgggcg
//
LOCUS       DATDAY010000067         4461 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_958015, whole genome shotgun sequence.
ACCESSION   DATDAY010000067 DATDAY010000000
VERSION     DATDAY010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 4461)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4461)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4461
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_958015"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1784)
                     /locus_tag="VIS74_01340"
     CDS             complement(<1..1784)
                     /locus_tag="VIS74_01340"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEY5791910.1"
                     /translation="MALGEFEHPGFLALLLALPALFLISRHSLANLPPRRARIALVLR
                     GLLLALLAFALAGPHFLAPAREPNVIFLVDDSRSADEPARARVEAWLAEAEKARPAGR
                     KKAVMRFARDLQIVRGFSGEAGPSSEDPRDATRITRAVRQAAALFPSGPGRIVVFSDG
                     LETEAVTPLEDLPPGREVDAVRLPADTRPDAWVEEIVAPVQVRAAESFNARVLAGSNV
                     PGTRAEVALFQDGFRIGQQSVTLAKGVQPVIFPNLRAAAAVSRLEATVALPEDAQPRN
                     DRLAALVASRGEPRALLIDPEPERLDPLAEVLRGARMEAEVRPPAGFPSEMKDLRGFD
                     LVILSDTSADSLRGEKTAVLRSWVEDFGGGLIVTGGPQSFGVGGYFRTPLEELLPVGS
                     EHPDKLELPVAALELVLDRSGSMTAVVGAQTKMDLANQGAVQALERLQNRDYFGVMAV
                     DVRPQTVVPLAPIGDRPAASRAIRGIRSSGGGIYVYSALLEAYRQLRTVPAKVKHVIL
                     FADAADAEEKSAAGGQSAEELAAVALGDRVTTSVVALGNEADKDAAFLRALAQAGGGR
                     FYLTHDATALPQLFAQETLRATQPSLVE"
     gene            complement(1800..2852)
                     /locus_tag="VIS74_01345"
     CDS             complement(1800..2852)
                     /locus_tag="VIS74_01345"
                     /inference="COORDINATES: protein motif:HMM:NF024937.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4126 domain-containing protein"
                     /protein_id="HEY5791911.1"
                     /translation="MEQTLQLLGLALGLASLAGLNLYLTVFATGLALRMDWVQLSPAY
                     AQLQVLEHPVVLGISGALFLAEFVADKVPWFDSVWDSVHTSIRPIGAALLAVCALGDA
                     NPIYEVAVGMLAGTAALTTHGLKSGTRLLANASPEPFSNVGLSLAGDGVVLGGLGLLA
                     FHPLTGLGIFLAVAALLVFLLPKVIRKALTRFQFAWSKLNTPAADQPPGWPPTQIPIS
                     AECELHRVHPTACEIAWVLPCVSRKGVNLAPDRRGWLAGIGGTEGSALYFIWKALFRT
                     ICVRLPVGGRPQAEFKPGFLYDSLTLRAEDGAMAAAFLVDRQHRETARRAAENLNQPP
                     RSNPAEAPVPSDLAEA"
     gene            complement(3055..3903)
                     /locus_tag="VIS74_01350"
     CDS             complement(3055..3903)
                     /locus_tag="VIS74_01350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5791912.1"
                     /translation="MKHITKLIAVAAVAGLVATSPAFAGTGKSLKEEVTVVEPTKWWG
                     AALSTGWDSLYMFRGVNVLGSTNNPYGSSLYWTNLSLSFNLTESDVITLGSWFATGLT
                     RNAYKELDISVAYTHSWGNLSLTLGYIYYYVISPTALFSHELSAGLGYTFDFGAFSIT
                     PSLTYFFNLGPQVGDDSGIAEQLSSYLQAKIVADIPIYKEAVSLAPWVALGTNFRYNF
                     RTYDDGGVAPFIGTNNLELGLAIPWAVNDIITVSVYGAYSYQWYGLIGTDPSTFWGGA
                     NVTFSF"
     gene            complement(4086..>4461)
                     /locus_tag="VIS74_01355"
     CDS             complement(4086..>4461)
                     /locus_tag="VIS74_01355"
                     /inference="COORDINATES: protein motif:HMM:NF019998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HEY5791913.1"
                     /translation="FIGDTNIVEARVESLTRGLLSAEAEGGLSLKVKTGVKEAGPDIL
                     LSLRPEKIQLFREKPPGDNVFPGTISLEIFKGATDELVVKTQSGLELSATSANDGNGQ
                     EFHEGDAVYFRIQPEDICLVRS"
BASE COUNT          920 a         1444 c         1324 g          773 t
ORIGIN      
        1 tcctcgacca ggctcggctg ggtcgcgcgc agggtttcct gggcaaacaa ctgcggcagc
       61 gcggtcgcgt cgtgggtcaa ataaaaccgc ccgccgccgg cttgcgcgag cgcccgcagg
      121 aacgcggcgt ccttgtccgc ctcgtttccc agggccacca cggaggtcgt aacgcgatca
      181 cctaatgcca cggcggcaag ttcttccgcc gattgtccgc ccgccgcgct cttttcctcc
      241 gcatccgcgg cgtccgcgaa caaaatcaca tgcttcacct tcgccggcac agtccgaagc
      301 tggcggtaag cctccagcag cgcggaatac acatagatgc cgcccccgga ggagcgaatg
      361 ccgcgaatcg cccggctggc tgcgggccgg tcgccaatcg gtgccagggg aaccaccgtc
      421 tgcggccgca catccacggc catgacgccg aaataatccc ggttttgcag ccgttccaat
      481 gcctgcaccg ctccttgatt ggcgagatcc atcttggttt gcgcgcccac cactgccgtc
      541 atcgaaccgg agcggtccag gaccagctcc aacgcggcca ccggcagctc cagcttgtcc
      601 ggatgctcgg agccgaccgg cagcagttcc tccaacggcg ttcgaaaata accgcccacc
      661 ccgaaactct gcgggccgcc ggtcacgatc aatcccccgc cgaagtcctc gacccatgat
      721 cgcaggaccg ccgttttttc accccgcagc gaatccgcgg aggtatcgct caggatgacg
      781 agatcgaacc cccgcaagtc tttcatttcg ctggggaatc ccgcgggtgg acgaacctcc
      841 gcctccattc gcgcgccccg cagcacctcc gccagcggat cgagccgttc cggctcggga
      901 tcaatcaata acgcgcgcgg ttcgccgcgg gaggcgacca aagcggccag acggtcgttt
      961 cgcggctggg catcttccgg caaagccacc gtggcttcga gccgggagac ggccgcggcc
     1021 gcccggagat tcgggaaaat caccggctga acgccctttg ccaatgtcac cgactgctgg
     1081 ccgattcgaa atccgtcctg aaacagcgcc acttccgcgc gggtccccgg gacattggac
     1141 ccggcgagca cgcgcgcatt aaaactctcc gccgcacgga cttggacggg agccacgatt
     1201 tcctccaccc aggcatccgg acgggtatcc gccggcagcc gcacggcatc cacttcccgc
     1261 cccggcggca gatcctccag cggcgtgacg gcctccgtct ccaatccgtc cgaaaaaacg
     1321 acgattcgtc ccgggccgga tggaaaaagc gccgccgcct gccgaaccgc gcgggtaatc
     1381 cgggtcgcat cccgcggatc ctcggaagac ggaccggcct cgccggaaaa cccgcgaacg
     1441 atttgcagat cccgggcaaa gcgcatcacc gccttcttcc ttcccgccgg cctggccttt
     1501 tcggcctcgg ccagccaagc ctccacccgg gctcgcgccg gctcatccgc gctccgggaa
     1561 tcgtccacga gaaaaatcac attcggctcg cgcgcgggcg ccagaaagtg cggtcccgcc
     1621 agggcgaatg ccagcagcgc cagcagcaat ccccgcagga cgagcgcgat ccgggcgcgg
     1681 cggggcggga gattggccag cgagtggcgg gaaatcagaa aaagcgccgg gagggccagc
     1741 agcaatgcca gaaaacccgg atgctcaaat tcgcccagcg ccattccgtt tcacgcgcgt
     1801 caggcttcgg ccaggtccga gggaaccgga gcttccgccg ggttcgaccg cggcggttga
     1861 ttgaggtttt ccgccgcccg ccgagcggtt tcccgatgct ggcgatccac gagaaacgcg
     1921 gccgccatgg ccccatcctc ggcgcgaagc gtcaatgaat cgtataaaaa gcccggctta
     1981 aattccgcct gcggccgccc gcccaccggc aggcggacgc agatcgttcg aaataacgcc
     2041 ttccaaatga aatacaacgc ggatccttcc gtcccgccga tcccggcaag ccagccccgg
     2101 cgatccggcg ccagattcac gccttttcgc gagacgcagg gcagcaccca ggcaatttcg
     2161 caggcggtgg gatgcacgcg atgcagctcg cattcggcgg aaatgggaat ctgcgtcggc
     2221 ggccaccccg gcggctgatc cgccgccggg gtgtttaatt tgctccaggc gaattggaaa
     2281 cgggtcagcg cctttcgaat caccttgggc agcagaaaaa ccagcagcgc ggccaccgcc
     2341 agaaaaatgc ccagtcccgt cagcggatgg aaggccagca aacccaagcc gccgagcacc
     2401 acgccatcgc ccgccaggct cagtccgaca ttggaaaaag gctccggcga ggcattggcc
     2461 agcaggcgcg tgccgctttt caggccatgg gtggtgagcg cggcggttcc ggccagcatt
     2521 cccaccgcca cttcataaat cggattggca tcaccgagcg cgcagaccgc cagcaaagcc
     2581 gctccgattg gccggatgga ggtatgaacc gaatcccaga ccgaatcaaa ccacggcacc
     2641 ttgtccgcca cgaattcggc cagaaacaat gcgccggaaa tgcccaggac caccgggtgt
     2701 tccagcacct ggagctgggc ataagccggg gacaactgca cccaatccat ccgcaacgcc
     2761 agcccggtcg cgaagacggt gagataaaga ttcaagcccg ccagactggc caaaccgagg
     2821 gcgaggccca aaagctgcag ggtttgctcc atgcttcacg attagcacag gcgccggacg
     2881 gcggacaggg caattcaatc ggaagacggt tttatatttc cagggttcgc gggatcggga
     2941 aacgaaaccc gccttttcga ctcagtccct tcccggcccg ggtacaaaaa caaaccgccc
     3001 cggtcgaaac cggggcggtt gcttttttaa gctgtaggtg cgtgacctaa aatcttagaa
     3061 gctgaacgtc acgttcgcgc caccccagaa ggtggaggga tcggtgccga tcaggccata
     3121 ccactggtag ctgtaagcgc catacacgga aaccgtgatg atgtcgttaa ccgcccaggg
     3181 aatcgcaaga ccgagttcga ggttgttcgt accaatgaac ggagcgacgc cgccgtcgtc
     3241 ataggtgcgg aagttgtaac ggaagttcgt tccgagagcc acccagggag cgagggaaac
     3301 cgcttccttg tagatcggga tgtccgccac gattttggcc tggaggtagc tggagagctg
     3361 ctccgcgatg cccgagtcgt cgcccacctg gggacccagg ttgaagaagt aggtcaagga
     3421 cggagtgatg ctgaacgcac cgaagtcaaa cgtgtaacca agaccggcgc tcagctcatg
     3481 cgagaagaga gcggtcgggc tgatcacgta gtagtagatg tagcccaagg taagcgagag
     3541 atttccccag ctgtgcgtgt aggcaacgga gatatcgagc tccttgtagg cgttacgggt
     3601 caggccggtg gcgaaccagg aaccgagggt gatgacgtcg ctctcagtga gattgaagct
     3661 cagcgagagg ttcgtccagt aaaggctcga tccgtaaggg ttgttggtgc ttccgaggac
     3721 gttcacgcca cggaacatgt agaggctgtc ccagcccgtg ctgagagcgg cgccccacca
     3781 tttcgtgggt tcaacgacgg tgacttcttc cttaagcgat ttgccagtgc cagcaaacgc
     3841 agggctggtg gcaaccagtc cggcaacagc ggcaacagca atcaatttag tgatgtgttt
     3901 catttgtttt ttccttcttt gttttttagt tccgcgcttt ggatgattgg cttgtgacag
     3961 cgtcacttgc cgctcttcct acagcatggt ttgtgccaag aatcagaatt tggaagggca
     4021 agtcaaataa ttttgaaaaa aatttgaacg tttttcgaca aagattgtct gccccatggg
     4081 ctcaatcagg atcgaaccag gcaaatatct tcgggctgaa tccgaaaata gacggcgtcc
     4141 ccctcgtgga actcctgccc atttccatcg ttcgcgctgg tggcgctgag ctccaggccg
     4201 ctttgggttt tgaccacaag ctcgtcggtc gcccccttga agatttccag tgagatggtg
     4261 ccgggaaaca cgttgtcgcc gggaggcttt tcgcggaaga gctggatctt ctccgggcgc
     4321 agcgaaagca aaatgtccgg cccggcttcc ttgacgccgg tcttgacctt cagcgacagc
     4381 cccccctccg cctccgccga aagcaggccg cgcgtcaggc tttccacccg ggcttcgacg
     4441 atgttggtat ccccgataaa g
//
LOCUS       DATDAY010000068         4298 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_766936, whole genome shotgun sequence.
ACCESSION   DATDAY010000068 DATDAY010000000
VERSION     DATDAY010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 4298)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4298)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4298
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_766936"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..775
                     /locus_tag="VIS74_01360"
     CDS             <1..775
                     /locus_tag="VIS74_01360"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HEY5791914.1"
                     /translation="LNWFESKPLYGKRIAVTRTRKQAGELIEQLRELGADAYELPTIR
                     IEPPTDLREFYELVAYAHSYDWLIFTSPNGVDAFFEAFFKIYRDARELGGVKIAAIGP
                     ATAARVASFHLQVDLQPDKYVAEAVVKAFQKTESVENLKILLARAENARDVLPVELSR
                     LGAIVDEAIAYRTVPETDAEEGIRRYREEGADLVTFTSSSTAENFAALKLPAPAAAAS
                     IGPITSATLRKLGLPLAVEARTHDIPGLVAAIAKHWKNV"
     gene            786..1700
                     /locus_tag="VIS74_01365"
     CDS             786..1700
                     /locus_tag="VIS74_01365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5791915.1"
                     /translation="MNRVLPLLAALSFAPIFAGHTQPSAFDPEKLRLQVESLLRLRVQ
                     ARDTARTAAIAEIQKQAKTGNGAVSYYENAYTDLTFAGKPGGGQAKVDWGKNNADLLK
                     SPEFKAAAQLHLQYLALTLSRAGSKQPLDFVGSSMEYLKAYGHADAGLFARQAKAPED
                     QRRLLASPLKDSPIVKANRLQALLAELPDWEPAPGNPDGIFEKNVLGPLREARDPRLL
                     DAWALRMEMGDKAVAAQKLDSRKNEFLNLQFPNWRFARAEDLLILGRREEAANEMYLL
                     LQHYPSHPSFEKWGQRLLEILAPAPTGS"
     gene            complement(1756..>4298)
                     /locus_tag="VIS74_01370"
     CDS             complement(1756..>4298)
                     /locus_tag="VIS74_01370"
                     /inference="COORDINATES: protein motif:HMM:NF014585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HEY5791916.1"
                     /translation="KIESVAAGGGSICLQDGIRLRVGPESAGADPGPACYGRGGPLTL
                     TDLNLLLGRLDPERFGVPIDPAAARVAFDQLHARVGGSAEALLQGLVQMADERMAASI
                     RQVTASEGFDAADYPLVAFGGAGGLHACGVAERLEMTRILFPADSGLLSARGLRAARR
                     EAFAERQILEPLERAGARLPEWSGALRAGAAKEIALEETGHRAEIELRLAGQEAALLL
                     PLAPAEKLPAAFARVYQKRFGYRPKDRAIEVVSLRVNVWEKKPRPPRETFARRRRLAV
                     NAPLDRAKLRADDWLNGPAIIQDAFSSLYVAKGWRATAGTRGSILLERIGSGSGKQAG
                     SGAVGLELMQNRLESIVEEMGGMLQRTAISTNVKERLDFSCALLDAEGRLIVNAPHIP
                     VHLGALGLCVRRVAARLPLGPGDAAVTNHPAFGGSHLPDVTVIAPIFDERGRRLGYVA
                     NRAHHAEIGGLLPGSMPPAARNLEEEGVVIPPMHLLRAGRPGFDAVEKLLRQARHPSR
                     RVEENLADLRAQVAAARRGAELLRGLAAKESPARVRKIFAAIRDRSARALAERLRECG
                     DFTREAEEFLDDGARLAVRLTHRAEWLTFDFSGTSPRHPGNLNATPAILRSVILYVLR
                     LLIPEPLPLNEGLLNRVKIKLPRCFLNPAFPADPAKCPPVVGGNTDTSQRLTDTLLKA
                     LGLAACSQGTMNNFLFGDAARSYYETIGGGGGAGPGFAGADAVHTHMTNTAITDPEIL
                     ELRYPVRLREFAIRPRSGGRGKWRGGNGVIREIEFLEPCEVSLLTQHRREAPYGMESG
                     QPGKRGAQTLILKSGAAAKLPSQAHFAAKRGDVLRIETPGGGGWGKAD"
BASE COUNT          738 a         1524 c         1373 g          663 t
ORIGIN      
        1 cctcaattgg tttgaatcca agccgcttta cggcaaacgc atcgcggtga cgcgcacgcg
       61 caagcaggcg ggcgaattga tcgaacaact ccgcgaactc ggcgcggacg cctacgagct
      121 gccgacgatc cgcatcgaac cgccgacgga cctgagggaa ttctacgagc tggtcgccta
      181 cgcgcattcc tatgactggc tcatcttcac gagcccgaac ggcgtggacg cgtttttcga
      241 agcctttttc aaaatctacc gggacgcgcg ggaactcggc ggcgtgaaga ttgcggccat
      301 cggcccggcc accgccgcgc gcgtggcgtc cttccatttg caggtggacc tccagccgga
      361 caaatacgtc gccgaggccg tggtgaaggc gttccagaaa acggagagcg tcgagaatct
      421 gaaaatcctg ctcgcccgcg cggagaatgc gcgcgatgtc ctgcccgtgg agctgtcccg
      481 gctcggcgcg atcgtggacg aagcgatcgc ttaccgcacc gtgccggaga ccgatgcgga
      541 ggaaggcatc cggcgctacc gcgaggaagg ggcggatctg gtaactttca ccagctcctc
      601 caccgcggag aatttcgccg cgttgaagct gcccgcgccc gcggcggcgg caagcatcgg
      661 acccatcact tcggccaccc tgcgcaagct ggggctgccc ctggccgtgg aagcgcggac
      721 gcacgacatt cccggattgg tcgccgccat tgccaagcat tggaagaacg tgtagccaag
      781 cggggatgaa ccgcgttctc cccctcctgg ccgcgctgtc gttcgccccg attttcgctg
      841 gccacaccca gcccagcgcc tttgatcccg aaaagctccg ccttcaggtg gaatccctgc
      901 tgcggctccg cgtccaagcc cgcgataccg cgcggacggc cgccatcgcc gaaatccaga
      961 agcaggcgaa aaccggcaac ggcgccgtga gttattacga aaacgcctac accgatctca
     1021 ccttcgccgg caaacccggc ggagggcagg ccaaggtgga ctggggaaaa aacaacgccg
     1081 acctgctcaa gagcccggaa ttcaaggcgg cggcgcaatt gcacctccaa tacctcgccc
     1141 tcacgctcag ccgggccggc tccaagcagc cgctcgattt cgtgggctcc tccatggaat
     1201 acctcaaggc ctacggccac gcggacgccg gcctgtttgc gcggcaggcc aaggcgccgg
     1261 aggaccagag acggctgctc gccagcccgc tgaaggacag cccgatcgtg aaggccaacc
     1321 gcctccaggc gctcctggcg gaattgccgg attgggagcc ggcgcccgga aaccccgacg
     1381 gcatcttcga aaagaacgtg ctgggccccc tgcgcgaggc ccgggatccg cggctgctcg
     1441 acgcctgggc gctgcgcatg gaaatgggcg acaaggctgt ggccgcccag aagctcgaca
     1501 gccggaaaaa cgaatttctc aatctccaat tccccaactg gcggttcgcc cgcgcggagg
     1561 atttgctgat tctcggccgc cgggaggaag cggcgaacga aatgtatctg ctcttgcagc
     1621 attatcccag ccatccctcc ttcgaaaaat gggggcagcg gctgctggaa atcctcgcgc
     1681 ccgcgccaac cggttcctga gcctttcaca gaaggacgca acggaaagac ctttgtgcgc
     1741 tttgcggcct tcggttcaat ccgcctttcc ccagcctccc ccgcccgggg tctcgatccg
     1801 gagcacgtcc ccgcgcttgg ccgcaaaatg ggcttgcgac gggagtttgg ccgccgcgcc
     1861 ggattttaag atcaaggtct gcgcgccgcg ctttccgggc tggccggatt ccattccgta
     1921 aggtgcttcc cggcggtgct gggtgagcag cgaaacctca cagggctcga ggaattcgat
     1981 ctcgcggatg acgccattcc ccccgcgcca ctttccgcgg ccgccggaac gcggacggat
     2041 cgcgaactcg cgcaggcgca cggggtagcg caattcgaga atttccggat cggtgatggc
     2101 cgtattcgtc atgtgcgtgt gcaccgcgtc cgccccggcg aaccccggcc ccgcgccccc
     2161 gccgccgccg atggtttcgt aataactccg cgccgcgtcg ccgaagagga aattattcat
     2221 cgtcccctgg ctgcacgccg cgaggccgag cgccttgagc agcgtgtccg tgagccgctg
     2281 gctggtgtcc gtatttccgc cgaccaccgg cgggcacttc gcgggatcgg cggggaacgc
     2341 cggattcagg aagcagcgcg ggagcttgat tttgacgcgg ttgagcaggc cctcgttgag
     2401 cggcagcggc tcgggaatga gcagccgcag gacgtaaaga atcacgctgc gcagaatggc
     2461 cggcgtcgcg ttgagattgc cgggatgccg cgggctcgtg ccggaaaagt cgaacgtcag
     2521 ccattccgcc cgatgcgtga gccgcacggc caggcgggcg ccatcgtcga ggaattcctc
     2581 cgcctcgcgc gtgaagtccc cgcattcgcg caaccgctcc gccagcgcgc gggcggagcg
     2641 gtcccggatc gcggcgaaaa ttttccgaac ccgcgcgggg ctttccttcg cggccagccc
     2701 gcgcagcagt tccgcgccac gccgcgccgc cgccacctgg gcgcgcaggt cggccaggtt
     2761 ttcctccacc cgccgcgagg gatgccgcgc ctgccgcagg agtttctcca ccgcgtcgaa
     2821 tcccggccgg ccggcgcgca gcagatgcat gggcggaatg accacacctt cctcctccag
     2881 atttcgcgcg gcgggcggca tcgagcccgg cagcaggccg ccgatctcgg cgtgatgcgc
     2941 ccggttcgcc acgtaaccca ggcgccggcc gcgctcgtcg aaaatcggcg cgatgaccgt
     3001 gacatccggc aggtgcgatc ccccaaacgc gggatggttc gtcaccgccg cgtcccccgg
     3061 cccaagcggc aggcgcgccg ccacccggcg gacgcacaat cccagcgcgc caaggtgcac
     3121 gggaatgtgc ggcgcgttca cgatcaaacg cccctcggca tccagcagcg cgcaggaaaa
     3181 atccagccgc tccttcacgt tcgtggaaat ggccgtgcgc tgcagcatgc cgcccatttc
     3241 ctcgacgatg gattccagcc ggttctgcat cagctccagc ccgaccgccc cggaaccggc
     3301 ttgctttcca cttccggagc cgatgcgttc gagcagaatc gagccgcgcg ttcccgcggt
     3361 ggcccgccag cctttcgcga cgtagagcga gctgaaggcg tcctgaatga tggcggggcc
     3421 gttcagccag tcgtccgcgc ggagcttcgc gcggtccagc ggggcattca ccgccagccg
     3481 ccggcgccgg gcaaaggtct cgcgcggcgg acgcggtttc ttttcccaga cattcacccg
     3541 caggctgacg acttcgatgg cgcgatcctt cgggcggtaa ccaaagcgct tctgatacac
     3601 ccgggcaaag gccgcgggca atttttccgc gggcgcgagg ggcagcagca gcgcggcctc
     3661 ctggcccgcc aggcgcagtt cgatttccgc gcgatgcccg gtctcctcca gcgcgatttc
     3721 cttcgcggcc ccggcccgca gcgcgccgct ccactccggc agtcttgcgc cggcgcgctc
     3781 cagcggctcc agaatctgcc gctccgcgaa ggcctcgcgc cgggcggccc gcagcccgcg
     3841 cgcgcttaag agacccgaat cggcgggaaa aaggatgcgc gtcatctcca gccgttccgc
     3901 cacgccgcac gcgtgcaggc cgcccgcccc gccaaaggcc acgagcggat agtccgcggc
     3961 gtcgaatccc tcgctcgccg tcacctggcg gatggaagcc gccatgcgtt cgtccgccat
     4021 ttgaaccagc ccctgcagca atgcctccgc cgatccgccg acccgcgcgt gcaattgatc
     4081 gaacgccacg cgggcggcgg cgggatcaat cggcacgccg aaacgctccg ggtccagccg
     4141 gccgagcagc aggttgagat cggtgagcgt cagcgggccg ccgcggccgt agcaggccgg
     4201 gccgggatcg gcgcccgcgc tttccgggcc gacgcgaagg cgaatgccat cctgcaaaca
     4261 aatggacccg ccgcccgcgg cgacgctttc aattttga
//
LOCUS       DATDAY010000069         2268 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_416835, whole genome shotgun sequence.
ACCESSION   DATDAY010000069 DATDAY010000000
VERSION     DATDAY010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2268)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2268)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2268
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_416835"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..752
                     /locus_tag="VIS74_01375"
     CDS             <1..752
                     /locus_tag="VIS74_01375"
                     /inference="COORDINATES: protein motif:HMM:NF025274.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid permease C-terminal domain-containing
                     protein"
                     /protein_id="HEY5791917.1"
                     /translation="GVIALRLADPNRPRKFRVPGGLPWAAAASVVLVGGAFLIPGPWS
                     EKLPFLLIAIPILWIFNAFTLPLLGIAACGYLILNLPMTSILRFVAWLNIGLVIYCGY
                     GVAHSKLQQSKGTQLDSAEHKTNMALMGVMLAIVGVFLAIGVRALEIFEEYSIGQQPI
                     SKWQLLWERADSMFHYGAWMGVSWFFIVPLVGNGLCLCPLLMWRLSRKDTQTKIAALE
                     KRRKLALTVSAVIILGTTGYLIAVFGHRLVS"
     gene            759..1676
                     /locus_tag="VIS74_01380"
     CDS             759..1676
                     /locus_tag="VIS74_01380"
                     /inference="COORDINATES: protein motif:HMM:NF013292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase"
                     /protein_id="HEY5791918.1"
                     /translation="MSKSLFPALALHGGAGNFPRGKFSKNLQAKYEKTLLACLDKGWR
                     ILEKGGPALDAVEVVVRALEDSPLFNAGRGSVLNEKGRVECDAALMAGDGRAGAVAGV
                     CGIKNPISLARTLLQDERVVLLAGKGAERYARARGFKFVPESYFITPPRVAELKEARA
                     ANLVSLDHAKFGTVGAVARDRSGALAAATSTGGLTNKAAGRIGDSPIIGAGTYACNET
                     CAISCTGVGEIFIREVVGHEISARMRHGGQPLAAAAKAVVHDVLAPIGGVGGVIAVGA
                     DGAISLEFNSESLYRAWRSGRGQPRAGIF"
     gene            1831..>2268
                     /locus_tag="VIS74_01385"
     CDS             1831..>2268
                     /locus_tag="VIS74_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY5791919.1"
                     /translation="MAQVTLKNVHKIYPGEKQNGIPAIADFNLEVQDREFVVLVGPSG
                     CGKSSILRMIAGLEEISQGEALIGGRRVNDMPPKDRDVSMVFQNYALYPHLSVYDNLA
                     FSLKIRKSPKAEIHKRVMEAAAMLGIESCLDRKPQALSGGQRQR"
BASE COUNT          397 a          721 c          720 g          430 t
ORIGIN      
        1 tgggcgtgat cgcgctgcgc ctggccgatc cgaaccgccc gcgaaaattc cgcgttcccg
       61 gcggcctgcc ctgggcggcg gcggcttccg tggttctcgt gggcggcgcg tttttgattc
      121 ccggcccctg gagcgaaaag ctgccgttcc tgctgatcgc gattcccatt ctctggatct
      181 tcaatgcctt cacgctgccg cttctcggca tcgccgcctg cggatacctc atcctgaatc
      241 tgccgatgac ctccattctg cgcttcgtcg cctggctgaa tatcgggctg gtgatctatt
      301 gcggctatgg cgtggcccac agcaagcttc agcaaagcaa gggcacccaa ttggattccg
      361 cggaacacaa gaccaacatg gcgctgatgg gcgtgatgct ggcaatcgtg ggagtctttc
      421 tcgcaatcgg cgtgcgcgcc ctggagattt tcgaggaata ctccatcggg cagcaaccga
      481 tctccaaatg gcagctgctt tgggaacggg ccgacagcat gttccactac ggggcctgga
      541 tgggcgtctc ctggtttttc atcgtcccgc tggtgggcaa tggcctttgt ctctgcccgt
      601 tgctgatgtg gcggctctcg cgcaaggaca cccagaccaa gatcgccgcg ctggaaaaac
      661 ggcgcaagct ggccctcacc gtctcggcgg tgatcatcct gggcacgact ggatatctga
      721 ttgcggtgtt cggccatcgt ctggtttcct agggaggaat gagcaaatcc ctcttccccg
      781 ccctcgcgct gcatggaggc gccggaaatt tcccgcgcgg caagttctcg aaaaacttgc
      841 aggccaagta tgagaagacc ctgctcgcct gcctggataa gggctggcgg attcttgaaa
      901 aaggcgggcc ggcattggat gccgtggaag tcgtggtgcg ggcgctggag gactcgccgc
      961 tcttcaacgc cggccgcggc tcggtgctca acgaaaaagg ccgggtcgaa tgcgatgccg
     1021 ccctcatggc cggagacggc cgggcgggcg ccgtcgccgg ggtttgcggc atcaaaaatc
     1081 cgatctcact cgcgcggacc cttcttcagg acgagcgggt ggtgctgctt gcgggcaaag
     1141 gcgcggagcg ctacgccagg gcccgcgggt tcaagttcgt gccggaaagt tatttcatca
     1201 cccccccgcg cgtggcggag ttgaaggaag ccagggcggc caacctggtt tcattggatc
     1261 atgccaaatt cgggaccgtc ggcgccgtgg cgcgggaccg atccggcgcc ctggcggccg
     1321 cgacttcaac cggcgggctg accaacaagg cggccggacg gatcggcgac agcccgatca
     1381 tcggcgccgg cacctacgcc tgcaatgaaa cgtgcgccat ttcctgcacg ggcgtcgggg
     1441 agattttcat ccgggaagtg gtcggtcacg aaatttccgc ccgcatgcgc cacggcggcc
     1501 agccgctcgc ggccgccgca aaggccgtgg ttcacgacgt gctggcgccg atcggcgggg
     1561 tcggcggcgt gatcgcggtg ggagcggatg gcgcgatctc cctcgaattt aattccgaaa
     1621 gcctgtatcg cgcctggcgc tccggccgcg gccagccgcg ggcggggatt ttctaaagcg
     1681 ccgcgggcgg gctccggatt tccggcctgc aaatcccctt tgacccacgc gccgcgcttt
     1741 cttaggattc gcgcttcacc gacagccgga ggaccgccca tcctccggct taaatccccg
     1801 ggcagctctc gaaaatcaac cgctcgaaac atggcccagg tcacgctcaa aaacgtccat
     1861 aaaatttatc caggcgagaa gcagaacggc attcccgcca ttgccgattt caacctggag
     1921 gtccaggacc gcgaattcgt ggttctggtc ggcccctcgg gctgcggcaa atccagcatc
     1981 ctgcgcatga tcgccggatt ggaggaaatc tcccagggcg aggcgctcat cggcggccgg
     2041 cgcgtcaacg atatgccgcc gaaggatcgc gatgtctcca tggtgttcca aaactacgcg
     2101 ctgtatccgc acctgtccgt ttatgacaac ctggcgttca gcctgaagat tcgaaagtcc
     2161 cccaaggcgg aaatccacaa gcgggtgatg gaggccgcgg ccatgctcgg catcgaatcc
     2221 tgcctggatc gcaagcccca ggccctttcc ggggggcagc gccagcgg
//
LOCUS       DATDAY010000070         3077 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_1830512, whole genome shotgun sequence.
ACCESSION   DATDAY010000070 DATDAY010000000
VERSION     DATDAY010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 3077)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3077)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3077
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1830512"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..488
                     /locus_tag="VIS74_01390"
     CDS             <1..488
                     /locus_tag="VIS74_01390"
                     /inference="COORDINATES: protein motif:HMM:NF014886.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyruvate kinase alpha/beta domain-containing
                     protein"
                     /protein_id="HEY5791920.1"
                     /translation="MLSGETTVGKYPIDCVKIFDRVSRRIEASGGAGYAELAVLDDIR
                     QKTMASAVKLANSVTNSKIVVFTRRGFMADHASHLRPVSAPIFAFSPNEAVVRRLTLN
                     RGVHAIRMAFSTKPEHTVAEAEEYLKQQGLANAGDELIILSDIVTGEERFDSIQLRRV
                     V"
     gene            620..901
                     /locus_tag="VIS74_01395"
     CDS             620..901
                     /locus_tag="VIS74_01395"
                     /inference="COORDINATES: protein motif:HMM:NF016268.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HEY5791921.1"
                     /translation="MKFEWDSAKAHSNKQKHGISFNEAIKCFADEKAVVILDEKHSSQ
                     KELRFYLIGKGDLGRILTIRYTHRGQTIRLIGAGAWRGGRDLYNQINAQ"
     gene            898..1278
                     /locus_tag="VIS74_01400"
     CDS             898..1278
                     /locus_tag="VIS74_01400"
                     /inference="COORDINATES: protein motif:HMM:NF027067.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY5791922.1"
                     /translation="MKIRKPKFDSEGYQVNIEDLNGEPLPDLSKVKIISREKAGLPPA
                     AAFRSAVKVLKPQIPASYIKARKQAGLTQNEFAEMLGVAVGTYRQWEHGRRTPSGPAV
                     SLLSLIAAHPELVLELRRKAGTLS"
     gene            complement(1280..1355)
                     /locus_tag="VIS74_01405"
     tRNA            complement(1280..1355)
                     /locus_tag="VIS74_01405"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1319..1321),aa:Glu,seq:ctc)
     gene            1453..1701
                     /locus_tag="VIS74_01410"
     CDS             1453..1701
                     /locus_tag="VIS74_01410"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HEY5791923.1"
                     /translation="MLKVYIKPGCSWCIEAVEWLQKRGYAFEEIDVFDDPRDFDRMRQ
                     ISGQSRAPTLETEKGLVLADFDVKQLATFLAANELTPP"
     gene            1698..2516
                     /locus_tag="VIS74_01415"
     CDS             1698..2516
                     /locus_tag="VIS74_01415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5791924.1"
                     /translation="MTSHLLLVAGMGVLSFALRSFQFPLLHRMGTAGIFLTSFLAGWL
                     LGGALWIGVVFAAFWLLLPWLEILTHVRRLRLPIERALQSRTPPPRNTFPGFTEFSDE
                     AEQLGFEYAEDAGWTYNDHAHFFRLFYHAEKRIQAAICLVEQGDLAFYYLSLTTRGVD
                     ERVFVTWNYPFSYGLQLPPKIRLNRLQGEVSFEQLLERHESFLTAQKLPLEMIAEQPA
                     GSFRRKIQGDLHDQIIHNLNRGLLVRDGEKLIRYTLRGMFFLWLQFLRDLVRFS"
     gene            2513..>3077
                     /gene="deoC"
                     /locus_tag="VIS74_01420"
     CDS             2513..>3077
                     /gene="deoC"
                     /locus_tag="VIS74_01420"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HEY5791925.1"
                     /translation="MTPRELAPFIDHTLLKADAVEREVIQLCEEAERYGFASVCVNGA
                     WVPAVAAFPKVRTTSVVGFPLGAMDPAAKAAEAARAVELGADEIDMVLAIGWLKERRL
                     KAVEADIRAVVDASGGRIVKVILETCLLTDEEKRLACQLSVSAGAAFVKTSTGFGKSG
                     ATVGDIRLMRAEAGPRLGVKASGGVRDY"
BASE COUNT          592 a          945 c          920 g          620 t
ORIGIN      
        1 tcatgctcag cggcgaaacc accgtgggca aatatccgat tgattgcgtg aagattttcg
       61 accgcgtttc ccgccgcatc gaagccagcg gcggcgccgg ttacgccgaa ctggccgtgc
      121 tcgacgacat ccgccagaaa accatggcct ccgccgtgaa gctcgccaac tcggtcacga
      181 attccaagat cgtcgttttc acccgccgcg gtttcatggc ggatcacgcc agtcatttgc
      241 gcccggtgag cgcgccgatc ttcgcctttt cgcccaacga agccgtggtg cgccgcctga
      301 cgctcaaccg cggcgttcac gcgatccgca tggccttctc gaccaagccc gaacacaccg
      361 tcgcggaagc cgaagaatac ctcaagcaac aaggactcgc gaacgcggga gacgaactca
      421 tcatcctcag cgacatcgtg accggcgagg agcgcttcga ctcgatccag ctgcggcggg
      481 tggtgtaaat cgcggaaaat cgccgggagc ccaatgcgac ccggcgagct ttgtttgggt
      541 aatccaaagg ccgcgtgcgt ttatgtcatt gacgcacttt tgccttgccg ggtggttgcg
      601 tcaatgacac gctatttctg tgaagtttga atgggattcc gccaaagccc attccaataa
      661 gcaaaagcac ggaatttcct tcaatgaagc gatcaagtgc ttcgccgacg aaaaagcagt
      721 ggtcattctt gatgaaaaac acagcagcca gaaagagctt cgcttttacc tcattggcaa
      781 aggagacctt ggccgaattt tgaccatccg ctatacccac cggggtcaaa ccatccgcct
      841 gattggcgcc ggagcgtggc ggggcggcag agacctctac aaccaaataa acgcgcaatg
      901 aaaatcagga aacccaaatt cgattctgaa ggttatcagg tcaatatcga agacctgaac
      961 ggcgaacccc ttcccgacct ttcgaaggta aaaattattt cccgcgaaaa ggccggactg
     1021 cctcccgccg ccgcttttcg ctccgccgtg aaggtgttga aaccgcaaat tcccgcttcc
     1081 tacatcaagg ctcgcaaaca ggcgggactg actcagaacg agttcgcgga gatgctgggc
     1141 gtggccgttg gaacgtatcg ccaatgggag catggccgga gaactcccag cggtccggcg
     1201 gtctccttgt tgagcctgat tgccgcccat cccgagcttg ttttggaatt gcgccgcaag
     1261 gcgggaaccc tttcctgact ggcagcgcgt acgggagtcg aacccgtgta gcggccttga
     1321 gagggccgtg tcctaggcca ctagacgaac gcgccgtgga gtgggccggt tacgttagca
     1381 ttcccgcccg ccatggcaag ggatgatttc gccattggcc cgcctgccca ttttggcgta
     1441 gtttgaaatt tcatgctcaa agtttacatc aaacccggat gctcctggtg catcgaagcc
     1501 gtggaatggc tgcagaagcg cggttatgcg tttgaggaaa tcgacgtctt tgacgatccg
     1561 agggatttcg accgcatgcg ccagatctcc ggccaaagcc gcgcgcccac cctggaaacg
     1621 gaaaagggcc tggtgctggc ggacttcgac gtcaagcagc tggcaacctt cctggctgcg
     1681 aacgagctga ctccgccatg acgtctcacc ttttgctggt ggccgggatg ggggtgctga
     1741 gtttcgcgct ccgctcgttt cagttcccgc tgctgcaccg gatgggaacc gccggcattt
     1801 tcctgacgtc cttccttgcc ggctggctgc tcggcggcgc gctctggatc ggcgtcgtct
     1861 ttgcggcgtt ctggctgctg cttccctggc tggagattct cacccatgtc cgccgcctcc
     1921 gtttgccgat cgaacgcgcg ctccagtccc gcacgccgcc cccgcggaac acgttcccgg
     1981 gattcaccga gttttccgac gaggccgagc agttgggctt cgaatacgcc gaggacgcgg
     2041 gttggaccta caatgaccac gcgcatttct tccgcttgtt ttatcacgcc gagaaacgca
     2101 tccaggccgc catctgtctc gtcgaacagg gcgatctggc attttattat ctgtcgctga
     2161 ccacgcgcgg cgtggatgaa cgggtgttcg tcacctggaa ttatcccttt tcctacgggc
     2221 ttcagctgcc gcccaagatt cgtttgaacc gcctgcaggg agaggtctcc ttcgaacaat
     2281 tgctggagcg gcacgagagc ttcctcaccg cgcagaaact gcctttggaa atgatcgccg
     2341 aacaacccgc cggctccttc cggcggaaga tccaaggcga cctgcacgat cagatcatcc
     2401 acaacctcaa ccgcgggctg ctggtccggg acggtgagaa gctcattcgt tacacgctgc
     2461 gcggcatgtt ttttctctgg ctccagttcc tgcgcgatct cgttcgattc tcatgacgcc
     2521 gcgggagctc gcccccttca tcgatcacac cctgctgaaa gccgacgccg tggagcgcga
     2581 ggtcatccag ctctgcgagg aggcggagag atacggcttt gcgagcgtgt gcgtcaacgg
     2641 cgcgtgggtt cccgccgtgg cggcttttcc caaggtgcgg acgacctcgg tggtgggttt
     2701 tccgctcggc gccatggacc cggccgccaa ggccgcggag gcggcgcgcg ccgtggagct
     2761 cggcgcggac gagatcgaca tggtgctcgc catcggctgg ctcaaggaac gccggcttaa
     2821 ggccgtggaa gccgacatcc gcgcggtcgt ggacgccagc ggcggacgga tcgtgaaagt
     2881 gattttggaa acctgccttt tgaccgatga ggaaaagcgc ctggcctgcc agctgagcgt
     2941 gtcggccggc gcggccttcg tgaagacctc gacgggattc ggcaaaagcg gcgcgacggt
     3001 tggggacatc cggctcatgc gcgccgaggc cggaccgcgc ctgggcgtga aggcttccgg
     3061 cggcgtgcgg gattacg
//
LOCUS       DATDAY010000071         2991 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_620621, whole genome shotgun sequence.
ACCESSION   DATDAY010000071 DATDAY010000000
VERSION     DATDAY010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2991)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2991)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2991
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_620621"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            194..1321
                     /locus_tag="VIS74_01425"
     CDS             194..1321
                     /locus_tag="VIS74_01425"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEY5791926.1"
                     /translation="MCGFRPIHFLALFLLSTTLGWAQWNVVTQQGRRYVPLGDVANFY
                     RMSRPVGEGKGFSLIGPGKLLRGSIGGREVYLNNVKHVLCFEVESQNGQPLISAMDVT
                     KIIEPVLRPGKIKNATAIRTVILDAGHGGHDGGARGALGNEKNFTLDVALRAKRLLEA
                     SGCKVRLTRSSDVFIPLEQRVAIANQYPNGIFVSIHFNKGSTGSGTGVETYCLAPRGV
                     PSMDAESVSYNDLRQYPGHENDAENVALATACHSSMLRRLRVFDRGIKRARFVVIRDL
                     KIPGVLLEGGFVDNPVDARLIASPDYRQRMALAVLDGVNAYRRAVNGQPAFTPPTVIA
                     SADGAGDQPPLDRPIAPETGINSPGPRLRINGVEINPESTQ"
     gene            1318..2145
                     /gene="murI"
                     /locus_tag="VIS74_01430"
     CDS             1318..2145
                     /gene="murI"
                     /locus_tag="VIS74_01430"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HEY5791927.1"
                     /translation="MNSESSLSSPIGVFDSGIGGLTVAAALRRLLPAEKIFYLGDTAR
                     VPYGGKSRRTVERYSVEISGLLLAEGAKIIVVACNTASALALPKLQELFRAPVQGVIL
                     PGAKSAVAKTQAGKIGVIGTRATIRSGAYEQAIRQLQPDARVFSEACPLLVPLIEEGL
                     LEDPITDAVLRRYLDPLLAKGVDTLVLGCTHYPLLREAIARVAGSGIALVDSAENCAL
                     AVRELLRQQAIEAGNQQNGSLRVALTDASEGFLRVAEEALDLEIGDVELRAVQGVLG"
     gene            2192..>2991
                     /locus_tag="VIS74_01435"
     CDS             2192..>2991
                     /locus_tag="VIS74_01435"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEY5791928.1"
                     /translation="MLSLAESRLLCSRASVAGKWVDARSGKTVPVTNPATGEVLAEVP
                     DLSAEEVREAIAFAHAAMREWQKRSALERSRILRRWFDLMITHIDDLAKILTAEQGKP
                     LAEAKGEIAYGAAYVEWFAEEAKRVYGDTIPAPSNDRRIVVIKQPIGVCAAITPWNFP
                     NAMIARKIAPALAAGCAMIVKPAMQTPLSALAMAVLAEEAGVPPGLFSVVTGDAREIG
                     GEFTANPLVRKLTFTGSTGVGKILMKQCADTVKKVTMELGGNAPFIVF"
BASE COUNT          544 a          942 c          977 g          528 t
ORIGIN      
        1 aatctcttga aggatgtggt gaggctgaag atttgagttc atgccgcaag tatatactaa
       61 cacatatact ccgcgacaaa aaagagaggg ggaaaaaacg tcccgataag aattttgtca
      121 tgcacccatc ccggaggaac cccgcaaaat gcacttgcct tcccggtttc ccgcgggaag
      181 gtagtaagtt tccatgtgcg gcttccgccc gattcatttc ctcgcgctgt tcctcctgtc
      241 caccaccctg ggctgggcgc agtggaatgt ggtgacgcag cagggccggc gctacgtgcc
      301 gctcggggat gtggcgaatt tttaccggat gtcgcgcccg gtgggcgaag gcaaaggctt
      361 cagcctcatc gggcccggca aattgttgcg cggctccatt ggcggccggg aagtttacct
      421 caacaacgtc aagcacgtcc tctgcttcga ggtggagtcc cagaatggcc agccgctgat
      481 ttccgcgatg gatgtgacca agatcatcga gccggtcctg cggccgggaa aaatcaagaa
      541 cgccacggcg attcggaccg tgatcctcga cgccggccac ggcgggcacg acggcggcgc
      601 gcggggagcg ctggggaacg aaaaaaactt cacgctcgat gtcgcgctgc gcgccaagcg
      661 attgctcgaa gccagcggct gcaaggtgcg gctgacccga agctcggatg tgttcatccc
      721 gctcgagcag cgggtggcca tcgccaacca atatccgaac ggcatttttg tgagcatcca
      781 tttcaacaag ggcagcaccg gctcgggaac cggcgtcgag acctattgcc tggcgccccg
      841 cggcgtgccg tccatggatg cggaaagcgt cagttacaac gacctgcggc agtatccggg
      901 ccacgagaac gatgcggaaa atgtcgcgct cgccaccgcg tgccattcct ccatgctgcg
      961 gcgcctgcgg gtttttgacc gcggcatcaa acgcgcgcgg tttgtggtca ttcgcgatct
     1021 caaaatcccc ggcgtgctgc tggaaggcgg gtttgtggac aatcccgtgg acgcccgcct
     1081 gatcgcgagc ccggattacc gccagcgaat ggctctggcc gtgctggatg gcgtgaacgc
     1141 ctaccgccgc gcggtgaatg gccagccggc cttcacgccg cccacggtga tcgcgagcgc
     1201 ggatggcgcc ggcgaccagc cgccgctcga ccggccaatc gcgccggaga ccggaatcaa
     1261 ttctccgggc ccgcgcctgc gcatcaacgg agttgaaatc aatcccgaat ccacccagtg
     1321 aattccgaat ccagcctgtc ctcgcccatc ggcgtttttg attccggcat cggcggcctg
     1381 accgtcgccg ccgcgctgcg ccggttattg cccgcggaga aaattttcta tctcggcgac
     1441 accgcgcgcg ttccctacgg cggcaagagc cgccggaccg tcgaacgtta cagcgtggag
     1501 atcagcggcc tgctgctggc cgaaggcgcg aagatcatcg tcgtggcctg caacaccgcc
     1561 tccgcgcttg cgctgccgaa attgcaggag ctcttccgcg cgccggttca gggggtcatc
     1621 cttcccggcg ccaagtccgc ggtggcgaaa acccaggcgg gcaagatcgg cgtcatcggc
     1681 acgcgcgcga cgattcgcag cggcgcctac gagcaggcca tccgccagct gcagccggac
     1741 gcccgcgttt tcagcgaggc ctgtccgctg ctcgtgccat tgatcgagga agggctgctg
     1801 gaggatccca tcaccgacgc ggtgctgcgg cgttacctgg atccgctgct ggccaaggga
     1861 gtggacacgc tggtgctggg ctgcacgcat tatcccctgc tgcgggaagc catcgcccgc
     1921 gtcgcgggat ccgggatcgc gctggtggat tcggcggaga attgcgcgct cgccgtccgg
     1981 gaactgctgc ggcagcaggc catcgaggcc ggcaatcagc aaaatggctc gctgcgcgtg
     2041 gctttgaccg atgcgagcga gggattcctg cgcgtggccg aggaggcgct ggatctggag
     2101 atcggcgacg tcgaactgcg cgccgtgcag ggcgtgctgg gttaatgccg gcgcgggttg
     2161 caatccccaa acgccgcgcc tagacttcat tatgctctcg ctcgccgaat ccaggctgct
     2221 gtgttccagg gcctccgtgg ccggaaaatg ggtggacgcc cgcagtggaa aaaccgttcc
     2281 ggtcaccaat ccggccaccg gcgaggttct ggccgaagtc cctgatttgt cggcggagga
     2341 agtgcgggaa gccattgcct ttgcgcatgc cgcgatgcgg gaatggcaaa agcgttctgc
     2401 cctggagcgc tcccggattc tgcgccggtg gttcgatctg atgatcaccc acatcgacga
     2461 tctggcgaaa atcctcaccg ccgagcaggg caagccgctg gcggaggcga agggcgaaat
     2521 cgcctacggc gccgcctacg tcgaatggtt cgcggaggag gccaaacggg tgtatggcga
     2581 caccattccc gcgccatcga atgatcgccg gatcgtcgtc atcaagcagc ccatcggcgt
     2641 ttgcgcggcg atcaccccgt ggaattttcc gaatgcgatg atcgcgcgca agatcgcccc
     2701 cgcgctggcc gccggctgcg cgatgatcgt gaagcccgcg atgcagaccc cgctctcggc
     2761 cctggccatg gccgtgctgg ccgaggaggc gggcgtgccg ccggggctgt tctcggtggt
     2821 caccggcgac gcccgggaaa tcggcggtga attcacggcg aatccgctgg tgcgcaagct
     2881 gaccttcacc ggctcgaccg gcgtggggaa aattttgatg aaacaatgcg cggacaccgt
     2941 gaagaaagtc acgatggagc tcggcggcaa cgcgccgttc atcgtgttcg a
//
LOCUS       DATDAY010000072         2431 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_2418665, whole genome shotgun sequence.
ACCESSION   DATDAY010000072 DATDAY010000000
VERSION     DATDAY010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2431)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2431)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2431
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2418665"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..379)
                     /locus_tag="VIS74_01440"
     CDS             complement(<1..379)
                     /locus_tag="VIS74_01440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY5791929.1"
                     /translation="MTQESLAIDVRGITKAFDGHTVVNGLDMQVRRGEIYGFLGPNGS
                     GKTTFLRILCGLLKPDAGSGACLGYDVLRQSDVIKRHVGYMTQRFSLYEDLSIAENLR
                     FVADIYEMKNARTAVKEMLASLGL"
     gene            complement(376..1284)
                     /locus_tag="VIS74_01445"
     CDS             complement(376..1284)
                     /locus_tag="VIS74_01445"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEY5791930.1"
                     /translation="MTKLLRLLLLLAVIAAAIYGARALFFRGPAAGDVYPGYGEGELV
                     YVAAPIGGEVVKLPVRRGDPVPAGALMFELDRTQELAARAEAEETLRQTRTEFDKAKL
                     DFDRAKTLRDKRVTAPEDYDHAVQALLAAQHAAAARQRALEQADWRYAQKQQTAPGGG
                     VVYDTYFRVGEWVPEGKPVLSLLLPEYRKVRFFVPERDLGKVQPGTPIEIHLDGLPAP
                     LPGKVSFVSPEAEYTLPIIYSRENREKLVFLVEGSLNPEDARLIHPGAPVEVRLANDR
                     MTSRAKQDSLPAGSPAGVEQSGNKPE"
     gene            complement(1281..1898)
                     /locus_tag="VIS74_01450"
     CDS             complement(1281..1898)
                     /locus_tag="VIS74_01450"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEY5791931.1"
                     /translation="MLVSNVIAQRIIETAEQTFLQRGFARVSMDELADSLGMSKKTLY
                     QHFSGKDDLLAAVLRHRTETVGRSILGILESAAPFPEKFRDLLNVIQRRMNEIQPVFL
                     EDLQRYAPEHFRIIEEFRARMLPVFFGKILDEGAAAGLIRGDLDRALFIRIAVNAIQS
                     LVRPGILTELKMHPWEGIDGILQIFFEGILTPQGRQTCGRYFLKS"
     gene            complement(2084..>2431)
                     /locus_tag="VIS74_01455"
     CDS             complement(2084..>2431)
                     /locus_tag="VIS74_01455"
                     /inference="COORDINATES: protein motif:HMM:NF013593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsH"
                     /protein_id="HEY5791932.1"
                     /translation="LARDMSRGRDYSEATAQQIDAEVKRLIDEAYQHATEILEKNRDK
                     VEVIAKALLEYETLDASHVKEIIEFGSIQNPPLKEVQPPPLPPPPMSDAPPAPEKPER
                     NFPAGGLPAPSPA"
BASE COUNT          434 a          754 c          762 g          481 t
ORIGIN      
        1 ccagccccag gctcgcgagc atttccttca cggccgtccg cgcatttttc atctcgtaga
       61 tgtcggcgac gaaccgcagg ttttcggcga tggagagatc ctcatacagg ctgaagcgct
      121 gcgtcatgta accgacgtgg cgcttgatca catcgctttg ccggaggaca tcataaccca
      181 ggcaggcgcc gctgcccgca tccggcttca gcagcccgca cagaatgcgc aggaaggtgg
      241 tcttgccgct gccattgggc ccgaggaagc cgtagatttc cccgcggcgg acctgcatgt
      301 cgaggccgtt gacgacggtg tggccatcga aggctttcgt gatgccgcga acgtcaatgg
      361 ccagcgattc ctgcgtcatt cgggtttgtt gccgctttgc tccacccctg cggggctgcc
      421 tgccggcaga ctgtcttgct tcgctcggct tgtcattcgg tcattcgcca aacgcacttc
      481 caccggcgcg ccgggatgaa tcaaacgcgc gtcttccgga ttaagcgaac cttccacaag
      541 gaacaccagc ttctcccggt tctcgcggct gtaaatgatc ggcagcgtgt attccgcctc
      601 cggcgagacg aagctgacct tgcccggcag gggcgcgggc aggccgtcga ggtggatctc
      661 gatcggcgtg ccgggctgga ccttgcccag atcgcgctcc ggcacaaaga agcgcacctt
      721 gcggtattcc ggcagcagga gggaaagcac gggtttgcct tccggcaccc attcccccac
      781 tcggaaatac gtgtcgtaaa ccacgccgcc gcccggcgcg gtttgctgct tttgcgcgta
      841 acgccaatcg gcctgctcca gcgcgcgctg gcgcgcggcc gcggcgtgct gggcggcgag
      901 cagcgcctgg accgcgtgat cataatcctc gggggcggta acccgtttgt cccgcaaagt
      961 tttcgcccgg tcgaaatcca gcttggcctt gtcgaattcc gtgcgggtct gcctgagggt
     1021 ttcctccgct tcggcccgcg cggccagttc ctgcgtgcga tccagctcga acatcaaggc
     1081 gcccgccggg accggatcgc cgcgccggac gggcagcttg accacctcgc cgccaatggg
     1141 cgcggccacg taaaccaatt ccccttcgcc gtagccgggg taaacgtccc cggccgcggg
     1201 accgcggaaa aagagggcgc gcgcgccata aatggcggcg gcgatgacgg ccagcagaag
     1261 caggaggcgg agcaatttgg tcatgatttc agaaaatacc ggccgcacgt ttggcggcct
     1321 tggggagtga ggatgccctc gaagaagatt tggaggatgc cgtcgatgcc ctcccagggg
     1381 tgcatcttta gctccgtgag aatgccgggc cgcacgaggg actgaatggc attcaccgcg
     1441 atgcggatga acaaagcccg gtcgagatct ccccggatga gcccggcggc ggcgccctcg
     1501 tcgagaatct tcccgaagaa aaccggcagc atgcgtgcgc ggaattcctc gatgatccgg
     1561 aaatgctccg gcgcgtaacg ctgcaaatcc tccaggaaaa cgggctgaat ctcgttcatg
     1621 cgccgctgga tgacattcag caaatcccgg aacttctccg gaaagggggc ggccgattcg
     1681 agaatgccca gaatgcttcg cccgacggtt tccgtgcggt gcctgagcac ggcggccagc
     1741 aggtcgtcct tgccggaaaa atgctgatag agcgtctttt tgctcatgcc aagcgaatcg
     1801 gccaattcat ccatggaaac ccgggcaaac ccccgctgga ggaaggtttg ttccgcggtc
     1861 tcgataatgc gttgcgcgat cacgttggaa actaacatcg ttttttaagt ttcccgcgtc
     1921 aagtttaaag cgacagtgat taccaaccct cgataccgcc gcgttggcat gaatgggaaa
     1981 atctggcccc gttccccgag ccgttgacca atccgaaatc ggttcgccgg acaaaaaagc
     2041 cgctcattcg agcggctttt tttgatcgtc aattcaggcg cgcctatgcc ggcgaaggag
     2101 ctggcaggcc gccggcgggg aaattccgct cgggcttttc gggcgccggc ggggcgtcgc
     2161 tcatgggcgg cggcggcagc ggcggcggct gcacttcctt gaggggcgga ttctggatcg
     2221 aaccgaattc gatgatctcc ttgacgtgcg aggcgtcgag ggtttcgtat tccagcagcg
     2281 ccttggcaat gacttcgacc ttgtcccggt tcttttccag gatttccgtg gcgtgctggt
     2341 aggcttcgtc aatcaaccgc ttgacctcgg cgtcgatctg ctgggccgtg gcttcgctgt
     2401 agtcgcgtcc ccggctcatg tcgcgggcga g
//
LOCUS       DATDAY010000073         3015 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_858273, whole genome shotgun sequence.
ACCESSION   DATDAY010000073 DATDAY010000000
VERSION     DATDAY010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 3015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3015
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_858273"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..105)
                     /gene="ssrA"
                     /locus_tag="VIS74_01460"
     tmRNA           complement(<1..105)
                     /gene="ssrA"
                     /locus_tag="VIS74_01460"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(277..753)
                     /locus_tag="VIS74_01465"
     CDS             complement(277..753)
                     /locus_tag="VIS74_01465"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY5791933.1"
                     /translation="MRIENVAHIPVEERHSPKGRFQVRQRHLSQALGAKKDTGTWGGG
                     HPFDVAQARIPPGKTNWPFHSHSAQWEFFLVQSGTGEVRTAEGIHPIQPGDCFVQEPG
                     TAHQITNPGGEDLVLLIVADNPPSDIVTYPDTGQYFLKPQRKLLKAGELPYYANEE"
     gene            863..2125
                     /locus_tag="VIS74_01470"
     CDS             863..2125
                     /locus_tag="VIS74_01470"
                     /inference="COORDINATES: protein motif:HMM:NF044863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5791934.1"
                     /translation="MNPPSHFRLTLPDLVDQLREAMEGAMTAEDIQKLCDLLYTASLL
                     KEEGRVVRARIMIAPPEAFASPEGPPDGIHAIRFTLPHELTAHEIKRLSPAASFFHSV
                     IAIWPERGRFRIWGILNTGPRWLNLVAGGRKPLGQDFTHPVIHVRDPGWLLFYRGYRL
                     MAEWRGRELHGPRLDVFQARLFVDRFRDLRRELVAEVHQSGLPATLSLEIYAELAHLI
                     ALQFIKRIINLVRTSGHGGSLIMLPEGPAGLAAATRWIDCKYSASAEPAGLRFRGLLQ
                     AIIRRVGELSPARTGLDAAWDLFRHSRDPELDRLEEAFFELARLFSDLMQVDGALVID
                     QRLCIIGFGGEIRVDRNLLKVGHAHDLNAAQVTEWSVRADGTRHRSVYRLCSVEPEVI
                     GFVISQDSQVRLIANVGGEVIFWMHTGV"
     gene            2170..2622
                     /locus_tag="VIS74_01475"
     CDS             2170..2622
                     /locus_tag="VIS74_01475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5791935.1"
                     /translation="MKRSRFFLPLFAAGALLLGGCASTEFTPWQGTAIEQGTGGTRKA
                     VNGIDVWQYGTPPLRYQILGLIEDKRDPDELFGDRLDDVTKKAREAGANGVILLDSSR
                     RQTGSYTTPSTTEIKVKNRDTVTATTTPGFTTVNYEDTVRFQAVKYLP"
     gene            2732..>3015
                     /locus_tag="VIS74_01480"
     CDS             2732..>3015
                     /locus_tag="VIS74_01480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5791936.1"
                     /translation="MQTGVRINPFSFLRVCAAGAALFLGLFPGAARAADLPPLEEITA
                     PSPMYARADEVWAEAKRLRLARDMDGLEALAAQLRQSKEALEGGPWLLSV"
BASE COUNT          490 a         1003 c          935 g          587 t
ORIGIN      
        1 gtgatctgcg tttatttgtt ttgactcgat ttttaacgag gccaacgggt catcctcggc
       61 atgcggactc ggtttcacct ttcaagtcga aaccattacg cccccatttg ggtctttcaa
      121 tatacccgcg tctgccggga actcaatgcg ttttatccgc gtttttatcc gcctccgccc
      181 gcaaccgccg cttgcttttc cggcattccc ggctacaatt tcgcgcaggt gccaaaacct
      241 tgaagcccgc ttctggaaac aggggcgggc tttattttac tcctcgttcg cgtaatacgg
      301 cagctccccg gccttcagca gtttcctttg gggcttgaga aaatactggc cggtgtccgg
      361 gtaggtcacg atgtccgacg gcggattgtc cgcgacgatg agcagcacga gatcctcgcc
      421 gcccgggttc gtgatctggt gcgccgttcc gggttcctgc acgaagcagt cgccgggctg
      481 gattgggtga atgccttccg ccgtgcgcac ttcgcccgtg ccggattgca ccaggaaaaa
      541 ctcccattgc gcgctgtgcg aatggaaggg ccaattcgtc ttgcccggcg ggatgcgcgc
      601 ctgcgccacg tcgaagggat ggccgccgcc ccaggtgccg gtgtctttct ttgcgcccag
      661 cgcctgggac aaatgccgct gcctgacctg gaaccggcct ttgggcgaat gccgttcctc
      721 gacagggatg tgcgccacgt tttcgatccg catgggcgga attctcccac gcgcgcgggc
      781 gaacgcaatg gcatttcgcg ccccgccctc cgcttttcgc tgggctccgg aaccgccgcc
      841 gggttagaaa aagaaagccg cgatgaatcc gccgtcccat ttccgtctca ccctgccgga
      901 cttggtggac cagttgcggg aggcaatgga gggggccatg accgcggagg atattcagaa
      961 actctgcgac ctgctctaca ccgcgagcct gctgaaggag gaaggccgcg tggtccgcgc
     1021 ccggatcatg atcgcgccgc ccgaagcctt cgcctcgccg gaggggccgc cggacggcat
     1081 tcacgccatc cgcttcaccc tgccgcacga gctgacggcc cacgaaatca agcgcctcag
     1141 cccggcggcg agttttttcc attccgtcat cgccatctgg ccggagcgcg ggcgctttcg
     1201 catctggggc atcctgaaca ccggcccgcg ctggctgaac ctcgtcgcgg gcggccgcaa
     1261 gcccctcggg caggacttca cccatcccgt catccacgtg cgcgatcccg gctggctgct
     1321 gttttaccgg ggttaccggt tgatggcgga atggcggggg cgcgagctcc atggcccgcg
     1381 gctggacgtt ttccaggcgc gtttgttcgt ggaccggttt cgcgacctgc gccgtgagct
     1441 ggtcgccgag gtccaccaaa gcggccttcc ggcgaccctc agcctggaga tttacgcgga
     1501 gctggcgcac ttgatcgcgc tgcaattcat caagcgcatc attaatctcg tccgcaccag
     1561 cgggcacggc ggcagcctca tcatgctgcc cgagggcccg gcggggctcg cggcggcgac
     1621 ccggtggatc gactgcaaat attccgcgtc ggccgagccc gccgggctgc gtttccgcgg
     1681 gctgctgcaa gccatcatcc ggcgcgtcgg cgagctgagc ccggcgagaa cggggctgga
     1741 cgcggcctgg gatttgttcc ggcacagccg cgatcccgag ctcgaccggc tcgaggaagc
     1801 cttcttcgag ctggcccggc tgttttccga cctcatgcag gtggacggtg ccctggtcat
     1861 cgaccagcgg ctttgcatca tcggcttcgg cggcgaaatc cgggtggacc ggaatctcct
     1921 gaaagtcggc catgcccacg atctcaacgc cgcgcaggtc acggaatgga gcgtccgcgc
     1981 ggacggcacg cggcatcgtt ccgtttaccg cctgtgctcc gtggagccgg aggtcatcgg
     2041 ctttgtcatc tcccaggaca gccaggtgcg cctcatcgcc aacgtgggcg gcgaggtcat
     2101 tttctggatg cataccggcg tttaagttgc aatggacaat ccggccgggc gcgtcctaga
     2161 ttttcgccga tgaaacgctc ccgatttttt cttccactct tcgccgccgg agctctcctg
     2221 cttggcggct gcgcttccac ggaattcacc ccctggcagg gaacggccat cgagcagggc
     2281 accggcggca cgcgcaaggc cgtaaacggc attgacgtct ggcaatacgg aacgccgccc
     2341 ctgcgttacc agattctcgg gctcatcgag gacaagcgcg atccggacga actcttcggc
     2401 gaccggctcg atgacgtgac gaaaaaggcc cgggaagccg gcgccaatgg cgtgatcctg
     2461 ctcgactcca gccgccggca gaccggttca tacaccaccc ccagcacgac ggagatcaag
     2521 gtaaagaatc gcgacacggt caccgcgaca accacgccgg ggttcacgac ggtcaattac
     2581 gaggacaccg tccgcttcca ggcggtgaaa tacctgccgt aaccccggtt tcgtcccggc
     2641 gcggcgggtc cggacgcgga gccatcccga aatttgttcg gagtctgaat ttttctgccc
     2701 ttttttgcgg aaacccatta gcgtgcggtg catgcaaacc ggcgttcgca tcaacccctt
     2761 ttcgtttctc cgagtctgcg cggccggggc cgccctgttt ctgggcctgt ttccgggcgc
     2821 ggcccgggcc gccgatttgc cgccgctgga agagatcacg gcgcccagcc ccatgtatgc
     2881 ccgggcggac gaagtgtggg cggaagccaa acgcctgcgc ctcgcgcggg acatggacgg
     2941 gctcgaagcc ctcgccgccc agctccggca aagcaaggaa gccctcgaag gcggcccctg
     3001 gctgctgtcc gtttt
//
LOCUS       DATDAY010000074         3680 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_1998418, whole genome shotgun sequence.
ACCESSION   DATDAY010000074 DATDAY010000000
VERSION     DATDAY010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 3680)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3680)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3680
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1998418"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1550
                     /locus_tag="VIS74_01485"
     CDS             <1..1550
                     /locus_tag="VIS74_01485"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="chitobiase/beta-hexosaminidase C-terminal
                     domain-containing protein"
                     /protein_id="HEY5791937.1"
                     /translation="GQIAEVLIYDRILTESERLQVEVYLADKYGMYHPNATWQQAYSA
                     AVQTEITRFKWKRSQADAYVAFLATNPPVPAPGLDLWLKADAGVTKDVDGKVSVWADQ
                     TANGNHASQASAGSQPVWVDEALGGKPVLSFGGTIQSMSVANAHNFDLAAPTIYVVGK
                     ATSGSYLAKNQANYVDGRRRKLQLKSDCFASGPDYATISYTAISQNYHILGVEGISNT
                     SQKIFVDGVENASTQTLDFTSYNEAGLLIGAAFSSGAEPLNGQIAEILVYDRILTESE
                     RLQVEVYLSQKYSLWLNVPAPSVSPGGGDYADSIPVTVTTPVPNGVIRYTLDGSDPQP
                     GSPVWTGALTLNGSANFKVRVFLPNGIAGQVASVQYYVKDTDQDGMSDAWEDAHGLDQ
                     DDAADAVLDEDGDGLTNLRECQLGTDPAEADSNDDGIPDGASFKAGLNPAVHDLDGDG
                     VSNANEIRAGTDLFLADSDGDGVNDGQDAFPLDPAHSTGSSANPNDHTAPAITLTRPA
                     GASLVDP"
     gene            1571..>3680
                     /locus_tag="VIS74_01490"
     CDS             1571..>3680
                     /locus_tag="VIS74_01490"
                     /inference="COORDINATES: protein motif:HMM:NF017411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5791938.1"
                     /translation="MRTFFPFIETLKTAFWLIVVFNGGLLLAEPEPQPAERTAEAAKS
                     AGQELGGARLARTRAASPEEERALKAAVDSFAARKKGDDFRAIEQYVESEKGSPYRLA
                     AMRDLGLQYYASGRFGKALQVWEEAWAAGKGSREPGVKEVIDDVFGELIRLHSRLGHL
                     ERLEALKKELGDRKLSGSAETKALNGWEAVWLMKSHPEASYRCGPLALGRVIALKKGD
                     PKVVVDEGVKDFLSTARGTSLEQVYEASRALGGGYQMAKRGPGAAMLLPAVVHWKVGH
                     FAAVTGRHGPNYVVQDSTFDDQFVFSEEALDEEASGYFLVPEGPLPPGWTPVGKEEAE
                     KVWGKGQPGNTQDKKNCPKGAGNQDCGGGSKKMAQYAVTLLTVGLNVRDTPLWYEPAY
                     GPEVHFTVQYNQRDAYQPVTLPYANVGRLWACNYVGFITFNPTVKPWPLSAQLYDGGG
                     SVTDFTFPPSSETTFQTTTSAVAEDGSVLKYLPAQSSGTSTTAAFERTFPDGSKQLYA
                     FADGSAGTVARSYLTGIVSPQGPQLNLVWEQSGGNFFLRKIQDAAGKETTFTYEANSV
                     YPTRIRGVQDPFGRSAAFTYDVLGRLSAITDMGGITSQFEYEAADDLIHALITPYGRT
                     QFSSGVSNQNAINRWIEITDPAGAKERVEFWNDINGLSGAVDLSDFDSGDMFPSGVSG
                     THFGNGGLLKMNTFYWNRKAM"
BASE COUNT          758 a          984 c         1244 g          694 t
ORIGIN      
        1 acggccagat cgcggaggtt ctcatctacg accggattct aacggagagc gaacgccttc
       61 aggtcgaagt gtatttggcg gacaagtatg ggatgtatca tccgaatgcg acttggcagc
      121 aggcctatag cgccgcggtg caaacggaaa tcacgcgctt caaatggaag cggagtcagg
      181 cggatgccta cgtggccttt ttggcgacca acccgccggt tccggcgccg ggcctggact
      241 tatggctgaa ggcggatgca ggggtgacca aggatgtgga tggcaaggta agcgtctggg
      301 cggatcagac ggccaacggg aaccatgcaa gccaggcttc agcggggagt cagccggtgt
      361 gggtggatga agcgctgggc gggaagccgg tgttaagttt tggtgggacg attcaaagca
      421 tgtcggttgc caacgctcac aatttcgact tggccgcacc gacgatttac gtcgtgggga
      481 aggcgacgag cgggagttat ctggcaaaga atcaagctaa ctatgtggat gggcggcggc
      541 gcaagctaca gttaaaatct gattgttttg ccagcggacc cgactatgca accatcagtt
      601 acaccgcaat ttcccagaac tatcacattc tgggggtgga aggcattagc aacacgtcgc
      661 agaagatttt cgtggatggg gtggagaatg cctctaccca gacgctggat ttcaccagtt
      721 acaatgaagc gggcttgctt attggagcgg ccttttcatc gggagcggag ccactgaatg
      781 gccagattgc ggaaattctc gtttatgacc ggattcttac ggagagcgaa cgccttcagg
      841 tcgaagtgta tttgagccag aagtatagtt tgtggttgaa tgtgccggcg ccgagcgtga
      901 gtccgggtgg gggagattat gcggattcga ttccggtaac cgtgacgacg ccggtcccga
      961 atggcgtgat ccgttacacc ctggatggaa gcgatccgca gcccggttcg ccggtttgga
     1021 cgggagcatt gacgttgaat ggctcggcaa acttcaaagt tcgggtattt cttcccaacg
     1081 gcatcgccgg acaggttgcg tccgtgcagt attacgtaaa agacacggat caggacggga
     1141 tgtccgacgc gtgggaggat gcccatggtt tggatcagga cgacgccgcc gatgcggtct
     1201 tggatgagga tggcgacggc ctgaccaacc tgcgggaatg ccagctggga accgatcccg
     1261 cggaggcgga cagcaatgac gacggcatcc cggacggcgc gtccttcaag gcggggctca
     1321 atccggccgt tcatgatctg gatggggacg gggtatcgaa tgcgaacgaa atccgggcgg
     1381 ggacggatct gtttttggcc gacagcgacg gggatggcgt caacgacggg caggacgcgt
     1441 ttccgctgga tccggctcat tcgaccggct cctccgccaa tcccaatgac cacaccgcgc
     1501 cggcgatcac ccttacccgg ccggccgggg caagcctggt tgatccctga tttttcccat
     1561 gatgcaacgc ttgagaacct tttttccatt catcgaaact ctgaagaccg cgttctggct
     1621 gatcgtggtc ttcaatgggg gcctgcttct ggctgagccg gagccccagc cggctgagcg
     1681 gacggccgaa gcggcgaaat ccgccgggca ggaattgggg ggcgcccgcc tggcgcggac
     1741 gcgggcggcc tcgccggagg aggaaagggc cctgaaggcg gcggtggact cgtttgcggc
     1801 ccgaaagaag ggagatgatt tccgggcgat cgagcagtat gtggaaagcg agaagggaag
     1861 cccctatcgc ctggcggcca tgcgcgatct ggggctgcag tattacgcca gcgggcgttt
     1921 tggcaaggcg ctccaagtct gggaggaagc gtgggcggcg ggcaagggct cccgggagcc
     1981 cggagtgaag gaagtgatcg acgatgtttt tggggagctg atccggctgc attcccggct
     2041 ggggcatctg gagcggctgg aagccctgaa aaaggagctg ggggatcgca agctgagcgg
     2101 cagcgcggag acgaaggcgc tcaatggctg ggaggcggta tggctgatga aaagccatcc
     2161 ggaagcctcg tatcgctgcg gtccgctggc cctgggccgg gtgatcgcct taaagaaggg
     2221 cgacccgaag gtggtggtgg acgagggagt gaaggatttt ctttccacgg cgcggggaac
     2281 gtccctcgag caggtttacg aagcatcccg cgcgctggga ggggggtatc agatggcaaa
     2341 acgggggccg ggcgcggcca tgctcctgcc cgcggtcgtg cactggaagg tgggccattt
     2401 tgcggcggtg accgggcggc acggcccgaa ttacgtcgtc caggacagca cgttcgacga
     2461 ccaatttgtg ttcagcgagg aagcgctcga tgaagaggcg agcggttatt tcctcgtgcc
     2521 ggaagggccg cttccccccg gctggacgcc ggttggcaag gaagaagcgg aaaaagtctg
     2581 gggaaaggga cagccgggca atacccagga taaaaaaaat tgccccaaag gcgccggcaa
     2641 tcaggactgc ggcggcggct ccaagaagat ggcccaatac gcggtgaccc tgctcacggt
     2701 gggattgaac gtgcgggaca cgccgctttg gtatgaaccg gcctacgggc cggaagtgca
     2761 tttcacggtg caatacaacc agcgcgacgc ctaccagcca gtcaccctgc cgtatgccaa
     2821 cgtgggtcgg ctgtgggcct gcaattatgt ggggttcatc acctttaatc ccaccgtcaa
     2881 gccctggccg ctttccgcgc agttgtatga cggcggcggc agcgtgaccg attttacgtt
     2941 cccgccttcc tcggaaacca cgttccagac caccaccagc gcggttgccg aggatggaag
     3001 cgtgctaaaa taccttccgg cccagagttc gggaacctcc acgacggcgg cctttgaacg
     3061 gaccttcccg gatggctcga agcagctcta cgcgttcgcg gacggttcgg cgggcaccgt
     3121 ggcgcgttcc tatttgacgg ggatcgtttc cccccaggga ccgcagctca acctggtttg
     3181 ggagcagagc ggcggcaatt tcttcctgcg aaaaattcag gatgcggccg gcaaggagac
     3241 gactttcacc tatgaggcaa acagcgttta tcccacccgc atccgcggcg tgcaggatcc
     3301 ctttgggcgc tccgccgcct ttacctatga cgtgctcggg cgcttgagcg cgatcaccga
     3361 catgggaggc attacctcgc aattcgaata cgaagcggcc gatgacctga tccatgccct
     3421 gatcacgccc tacggccgca cgcagttcag ctcgggggtg agcaaccaga atgcgatcaa
     3481 tcgctggatt gagatcaccg atccggccgg ggcgaaggaa agggtggagt tctggaacga
     3541 catcaacggc ttgagcggcg ccgttgattt aagcgacttt gactccgggg acatgtttcc
     3601 ttccggcgtg agcggaacgc attttggcaa cggcggcctg cttaagatga atacgttcta
     3661 ctggaatcgc aaggcgatga
//
LOCUS       DATDAY010000075         2687 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_2181880, whole genome shotgun sequence.
ACCESSION   DATDAY010000075 DATDAY010000000
VERSION     DATDAY010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2687)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2687)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2687
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2181880"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..547
                     /gene="mreC"
                     /locus_tag="VIS74_01495"
     CDS             <1..547
                     /gene="mreC"
                     /locus_tag="VIS74_01495"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HEY5791939.1"
                     /translation="DLESENNRLRDSLGYLERSQFRLVPAQVVSRDASTWWNTVRINR
                     GFEDGVEVDRPVITDLGLVGKTVSVSKNLSTVVLISDENCKVGARVEGTREQGITQGR
                     RISAESKGEIELNFLTKQADLKPGSVVVSAGVTGGVFPAGLKLGAIKSFRVRDLDGQA
                     VVEPAVDLSTVRDVFVIVSEK"
     gene            544..1104
                     /locus_tag="VIS74_01500"
     CDS             544..1104
                     /locus_tag="VIS74_01500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5791940.1"
                     /translation="MTRDAISCLGLCAAIFAALILQEFIPPLAGMEDARVVLAPMVVC
                     YAALALPFPFMLVAVFWAGFSLDFMNLQWVDGKPEIAPGATIFYFLGVGLLCQGLRVL
                     FLRGYWWLFSLMSGLATSLLLLLQFVFISYRRFETGGIEWSTLVLWRIGLPGLIAFLI
                     APIFHLLAYLTGRSLLPAVTRSRVFG"
     gene            1101..>2687
                     /locus_tag="VIS74_01505"
     CDS             1101..>2687
                     /locus_tag="VIS74_01505"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEY5791941.1"
                     /translation="MKRSSFYFRFYALAAIIIVLFGVLVVRLWVVQILQGTEYAAKIE
                     NRSQVTVRLPAVRGEILDRNGVKLVENRACYEVDFYLPAMVKAYRDKHEGKMPTTQYR
                     GRVRGMLRMKKAADMETIIRDDVIPRLEKLGLAQDYNSQRLQTHFQDSAEIPFSYRQN
                     IDYDTMVRYMENNVGLPGLEIAIKPMRHYIYGSLAAHMLGYVGAPLDHDPQEVAKYNY
                     FQPDVVGKSQVELFYDSYLRGTAGAKILDRDVKGVIRGELKRIEPRQGANVYLTIDAR
                     LQFIVEQTLRNVGRASAVVMDPNNGDILAMASVPAFDPNTFIPSISAADWAALQKNPA
                     NPLVNRAISAYAPGSTYKIPISLAGLRAGIGSRTFSCSGGVQYGNFYMKCWLKTGHGT
                     LRLEEAIKVSCNSFFYQYGNAAGINQIVAVGNMLGLGQKSGLPLSGEVPGILPGPEWL
                     AQNYPRERWSSGYTANAAIGQGFVLVSPLQMAVVASAVANGGTAYYPRLVEKVVSQDG
                     QVLLQEPARVRADLKTDAGMK"
BASE COUNT          466 a          785 c          882 g          554 t
ORIGIN      
        1 cgatctggaa tccgaaaaca accgcctgcg cgattcgctg gggtatctgg agcgttcgca
       61 attccggctc gttccggccc aggtcgtttc gcgcgatgcc tcgacttggt ggaatacggt
      121 gcgcatcaac cgcgggttcg aggacggcgt ggaggtggat cgcccggtga tcaccgatct
      181 cgggctggtg gggaagaccg tctcggtttc caagaatctt tccaccgtgg ttttgatttc
      241 ggatgaaaac tgcaaggtgg gggcgagggt cgagggcacg cgcgagcagg gcatcaccca
      301 ggggcggcgg atttcggcgg agagcaaggg ggaaatcgag ctgaattttt taaccaagca
      361 ggcggacctc aagccgggaa gcgtggtggt ctccgccgga gtgacgggcg gggttttccc
      421 ggccggcttg aagcttgggg cgatcaagtc ctttcgcgtg cgggatctgg acggccaggc
      481 ggtggtcgaa ccggcggttg atttgtccac ggtccgcgat gtcttcgtga ttgtgagcga
      541 aaaatgacgc gggacgccat cagttgcctg ggcttgtgtg ccgcgatctt cgccgcattg
      601 atcctgcagg agttcatccc gccgctggcg ggcatggaag atgcgcgcgt ggtgctggcg
      661 cccatggtgg tgtgttatgc ggcgctggcg ctgccctttc ccttcatgct cgtcgcggtt
      721 ttttgggccg gattcagcct ggacttcatg aacctgcaat gggtggacgg caaacctgag
      781 attgcccccg gagcgacgat tttttacttt ttgggcgtcg gtttgctttg ccaggggttg
      841 cgggttttgt ttttgcgcgg atattggtgg ctgttcagcc ttatgagcgg cctggccaca
      901 tcgctgctgc tgttgctgca gttcgtcttt atttcctacc ggcgttttga gaccggcggg
      961 atcgaatgga gcacgctggt gctctggcgg attggcctgc ccggcctgat cgcgttcctc
     1021 atcgcgccca tttttcatct gctggcttac ctgacgggcc ggagcctgct gccggcggta
     1081 acccgttccc gtgttttcgg atgaagcgct cctccttcta cttccgattt tacgcgctgg
     1141 cggcgatcat cattgtcctg ttcggggtgc tcgtcgtgag gctgtgggtg gtgcagatcc
     1201 tccagggcac ggagtatgcg gccaagatcg agaaccgttc ccaggtgacc gtccgcctgc
     1261 cggcggtccg gggggaaatc ctggatcgca acggcgtcaa gctggtggag aaccgggcct
     1321 gctacgaggt ggatttttac cttcccgcca tggtgaaggc gtatcgcgac aaacacgaag
     1381 gcaagatgcc gaccacgcaa taccgcggcc gggtccgcgg catgctgcgg atgaagaagg
     1441 ccgcggacat ggagacgatc atccgggacg atgtcatccc gcgcctcgaa aagctgggtt
     1501 tggcgcagga ctacaactcg cagcgcctgc agacgcattt ccaggacagc gcggagattc
     1561 ccttcagcta ccggcagaac attgattacg acaccatggt tcgctacatg gagaacaatg
     1621 tcgggctgcc cggcctggaa atcgccatca agccgatgcg ccattacatt tacggctccc
     1681 tggccgcgca catgctgggt tacgtgggcg ccccgctcga ccacgatccg caggaggtgg
     1741 cgaaatacaa ctatttccag ccggacgtcg tgggcaaatc gcaggtggaa ttattctacg
     1801 actcctacct ccgcggaacg gccggcgcca agatcctcga ccgcgacgtc aagggggtga
     1861 ttcgcggcga actcaagcgc atcgagccca ggcagggggc gaacgtgtat ctgaccatcg
     1921 acgcgcggct gcaattcatc gtcgagcaaa cgctgcgcaa tgtcggccgg gcctcggcgg
     1981 tggtgatgga tccgaacaat ggcgacatcc tcgccatggc ttcggtgccg gccttcgatc
     2041 cgaacacctt catcccctcc atctccgcgg cggactgggc cgcgctgcag aaaaatcccg
     2101 ccaatccgct cgtgaaccgg gccatcagcg cctacgcccc cggttcgacc tacaaaatcc
     2161 ccatcagcct ggccggattg cgcgcgggca tcggcagccg caccttcagt tgctcgggag
     2221 gcgttcaata cggcaatttc tacatgaaat gctggctgaa aaccggccac ggcacgctcc
     2281 ggctggaaga agcgatcaag gtctcctgca actcgttttt ctatcaatac ggcaacgccg
     2341 cgggcatcaa ccagatcgtc gccgtgggca acatgctcgg actggggcaa aagagcgggc
     2401 tgccgctctc cggcgaggtt cccggcatcc tgcccgggcc ggagtggctg gcgcagaact
     2461 atccgcgcga gcgctggtcc tcgggttata cggcgaacgc cgccatcggc caggggtttg
     2521 tgctggtctc cccgttgcag atggccgtgg tcgcctcggc ggtggccaac ggcggcacgg
     2581 cctattatcc ccgattggtg gagaaggtgg tttcgcagga cggccaggtc ttgctgcagg
     2641 agcccgcgcg ggtgcgtgcg gacttgaaga cggacgccgg catgaag
//
LOCUS       DATDAY010000076         2030 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_2091748, whole genome shotgun sequence.
ACCESSION   DATDAY010000076 DATDAY010000000
VERSION     DATDAY010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2030
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2091748"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..361)
                     /locus_tag="VIS74_01510"
     CDS             complement(<1..361)
                     /locus_tag="VIS74_01510"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEY5791942.1"
                     /translation="MPQVSDSVAHLLELIRFPSVSTQPEHAADLTACAQWLVDYLTRI
                     GLSAAPHPTGGHPVVVAKTPVNPAKRTVLIYGHYDVQPAEPLEEWQSPAFEPEIRNGR
                     IYARGSTDNKGQIFAHIT"
     gene            488..>2030
                     /locus_tag="VIS74_01515"
     CDS             488..>2030
                     /locus_tag="VIS74_01515"
                     /inference="COORDINATES: protein motif:HMM:NF014516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY5791943.1"
                     /translation="MYLQSLELHGFKSFAQKTTLQFHRGVTAIVGPNGCGKSNVLDAI
                     RWVLGEQSAKALRGGEMADVIFSGTDSRSALGMAEISMTFADCEKELGVEWNEVRITR
                     RVFRDGRGEYLLNKTPCRLKDIHQLFMDTGIGRSAYSIMEQGKIDQILSSRPEDRRAI
                     FEEAAGITKYKSQKKEALRKLEYTEANLLRVSDIIKEVKRQIGSLQRQAAKARRYQSL
                     TEGLKIFDTHLSHRQFVELDRERNSLTSQVNQIEESREVYETEISAQETELAGYRARL
                     NELDAEIGRARDHVQTLRSRIYSAESRIESHGQRTVEFRELIERNRADIAITEEKTRE
                     QESQLQRTNELIETLGHSLREAQDALSAQEQQAQSVRGERLQAEQQLQAAAREFSRAE
                     SDYSRLRGELTNLGARREGVEARLGMLNGESAVAGELLQDIEQRGIKVAERLRGVAAA
                     LGEGEAALALANEEQVQAQIEKEAIEAEAGQIDRRQAETHSRLQVLVQLEADGEGLGE
                     ATQAIL"
BASE COUNT          430 a          598 c          669 g          333 t
ORIGIN      
        1 cggtgatgtg ggcaaaaatc tggcctttgt tgtcggtgga accgcgggcg tagatccggc
       61 cgttccgaat ctccggctcg aaggccggcg actgccattc ctccagcggc tcggcgggct
      121 ggacgtcgta atggccataa atcaggactg tgcgcttggc cggattcacc ggggttttgg
      181 ccaccaccac cggatgcccc ccggtcggat gcggcgcggc ggacaggccg atgcgggtca
      241 ggtaatcgac cagccattgg gcgcaggcgg tcaggtcggc ggcgtgctcg ggctgggtgg
      301 aaacactggg aaaacgaatc aattccaata aatgggcaac cgagtcggaa acttggggca
      361 tatacaatat attgtggttt tttggttgtc ggcccacagc aaatggtatt tttccttgtt
      421 gcgggtttgg gattgggtta aaaaaaccgc acgccaacct gactcagcca accttaccga
      481 agagcttatg tatctgcaat ccttagaact gcacgggttc aaatcttttg cgcaaaagac
      541 cacgctgcaa ttccatcgcg gcgtgaccgc gatcgtcggg cccaacggct gcggcaaatc
      601 caacgtgctc gacgcgattc gctgggtgct cggcgaacaa tccgccaagg cgctccgcgg
      661 cggcgaaatg gcggatgtca ttttcagcgg gacggacagc cgctcagcgc tcggcatggc
      721 ggaaatttcg atgacgtttg ccgattgcga gaaggagctc ggcgtggagt ggaacgaggt
      781 gcggatcacg cgacgggtgt ttcgcgacgg gcgcggcgaa tatttgctca acaagacgcc
      841 gtgccgcctg aaggacattc accagctttt catggatacg ggcatcggcc gcagcgccta
      901 ctcgatcatg gagcagggca agatcgacca gatcctgagc agccgtccgg aagaccgccg
      961 cgcgattttt gaagaggccg ccggcattac gaaatacaag tcgcagaaaa aggaggcgct
     1021 ccggaaactc gaatacaccg aggcgaacct gctgcgcgtc tccgacatca tcaaggaagt
     1081 caaacgccag atcggctcgc tccagcgcca ggcggccaag gcgcggcggt atcaaagcct
     1141 cacggaggga ttaaaaattt tcgacaccca tttgtcgcac cggcaattcg tggagctcga
     1201 ccgcgagcgc aactcgctta cttcccaggt caatcagatc gaggaatccc gcgaggttta
     1261 cgaaaccgaa atctccgcgc aggaaaccga gctggcgggc taccgcgcgc ggttgaatga
     1321 gctggatgcg gagatcggcc gggcgcgcga ccacgttcag acgctgcgca gccggattta
     1381 ttccgccgag agccggatcg aatcccacgg ccagcgcacg gtggaattcc gcgagctcat
     1441 cgagcgcaac cgcgcggata tcgcaatcac cgaagagaaa acccgcgagc aggagagcca
     1501 gctccagcgg acgaatgaat tgatcgaaac gcttggccat tcgctgcgcg aggcgcagga
     1561 cgcgctttcc gcccaggagc agcaggcgca atccgtgcgc ggggagcgcc ttcaggccga
     1621 gcagcaattg caggccgccg cgcgggaatt ttcccgggcg gagtccgatt attcccgtct
     1681 ccgcggtgaa ctcaccaacc tcggcgcgcg gcgcgagggc gtggaagccc gcctgggaat
     1741 gctcaatggc gaatccgccg tcgccggcga gctgctccag gacatcgagc agcgcggcat
     1801 caaggtggcg gagcgcctcc gcggcgtcgc cgccgcgctg ggcgaagggg aggccgcgct
     1861 ggcgctggcg aacgaggagc aggtccaggc gcagatcgag aaggaggcca tcgaagccga
     1921 ggcggggcag atcgaccgcc gccaggcgga aacccattcg cgcctgcagg tgctggtgca
     1981 gctcgaagcc gatggcgaag gcctcggcga ggcgacgcag gcgatcctgc
//
LOCUS       DATDAY010000077         6849 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_768835, whole genome shotgun sequence.
ACCESSION   DATDAY010000077 DATDAY010000000
VERSION     DATDAY010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 6849)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6849)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6849
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_768835"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..951
                     /locus_tag="VIS74_01520"
     CDS             <1..951
                     /locus_tag="VIS74_01520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5791944.1"
                     /translation="EAAGNAPESSDPHGEFRGKNILIERESGGDFSAAREKLRLVRER
                     RPRPHLDDKILTAWNALMISAFAKAGSSLGEPAFLATATRAVNFLRENLWSGGELYRS
                     WRRGKRSTPAFAEDHAFLISALFDLYQSTFDPAHLTWARELQARQDALFWDDAIGAYY
                     SNRAGDSHLKVRMKEDYDGAEPAANSVSAENLLRLARMFHDEEAEERAQSIFRSLAPV
                     LNRIPHSVPQLLSAWMLAREPARQIVIAGGLDAPETRALLKPIRETFLPDAVVLHSSP
                     EMPEAIRLMRPVSGKPAVYVCENFRCLAPVTDPGQLRLTP"
     gene            complement(941..1846)
                     /locus_tag="VIS74_01525"
     CDS             complement(941..1846)
                     /locus_tag="VIS74_01525"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEY5791945.1"
                     /translation="MPQNLLGIDLGGTKIEAALLDSGQPDRPLHRLRVPTEQERGYDH
                     ILSQIEKACRTLVAEAGAELPPVIGIGHPGTLDPRTQVLKGSNTQCLNGKPLKADLET
                     RLGRVFELANDANCFALAEAAMGAAKGYETVFGIILGTGAGGGLVVRGKVLNGCHGIA
                     GEWGQIVLDPHGPVSVYGTRGTVEAYLSGPSLEKFYAGESGVRRGLKEIVERADAGAD
                     AVAAATLERLSDYFAKAIYQIIDVFDPHAIVIGGGVGNIPVLYTEATRQKIAERIFNP
                     TFEAALLKPVLGDSAGVFGAAMLTA"
     gene            complement(1873..2580)
                     /locus_tag="VIS74_01530"
     CDS             complement(1873..2580)
                     /locus_tag="VIS74_01530"
                     /inference="COORDINATES: protein motif:HMM:NF016921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MT-A70 family methyltransferase"
                     /protein_id="HEY5791946.1"
                     /translation="MSKVTSLTEPTPADDLLEKVQARYSTILADPPWQFQNRTGKVAP
                     EHKRLLRYPTMDLQEIIQLPVGHLAAAQSHLYLWVPNALLAEGLEVMRGWGFTYKSNL
                     VWYKVRKDGGPDGRGVGFYFRNVTELVLFGVRGSMRTLNPGRTQVNLFGTRKREHSRK
                     PDEIYDLIEKCSSGPYLELFARFRRPGWSQWGNEDVEENSFHGVAKRNGHHDSQLRLL
                     EAPAGYQPSQITGKKSK"
     gene            complement(2588..3325)
                     /locus_tag="VIS74_01535"
     CDS             complement(2588..3325)
                     /locus_tag="VIS74_01535"
                     /inference="COORDINATES: protein motif:HMM:NF020757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglII/BstYI family type II restriction
                     endonuclease"
                     /protein_id="HEY5791947.1"
                     /translation="MPAIFEKLKKRGFDVEVLHHAEAILLHDMPQALVELEGALLELQ
                     IPVLELVQGGGGEALVTQRLRRQLAELGWEKHNFVIKRTVDGIEKESRSHEIDHVKNF
                     SRGTVALEIEWNNKDPFFDRDLENFKTLHAEGAVSVGGIITRGRSLHQKMRDILRQFA
                     LNEEIDSVQDLARFELEPTRRQTGLYETAAKSKGEFAAGWAHAFVADKFGEATTHWSK
                     LEDRIRRGVGNPCPLVLIGIPDSVIVI"
     gene            complement(3331..4851)
                     /gene="proS"
                     /locus_tag="VIS74_01540"
     CDS             complement(3331..4851)
                     /gene="proS"
                     /locus_tag="VIS74_01540"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEY5791948.1"
                     /translation="MSSNEKTAISPRRDEDFPEWYQQAVRAAELAENSEVRGCMVIKP
                     WGYALWERMQAVLDGMFKDTGHKNAYFPLFIPLSHLQKEAEHVEGFAKECAVVTHHRL
                     ELVDGKLVPSGELNEPLIVRPTSETIIGATYAKWVNSYRDLPILINQWANVVRWEMRP
                     RLFLRTAEFLWQEGHTAHETEAEAVEETEKMLRVYETFARDYLAVPVICGEKTESERF
                     PGAVRTYCIEAMVQDRKAIQAGTSHFLGQNFARASGIQFQSREGKIEHAWTTSWGVST
                     RLIGTLIMTHGDDDGVIIPPKVAPSHVVILPVTPKEETREAVLAACEKLAADLRATSF
                     SGQPIRVEVDRRDAGGGVKNWDWVKKGIPLRVEIGPRDLEKGSMAVTRRDQPPKAKQF
                     LQIADFVTSVATVLTEIQQALLDRATAFRDENFKILGSKEAFYDYFTAKNPDKPEIHG
                     GFALAHWNGSAAVEEQIKNDLKVTIRCIPFEESPEPGVCIFTGEPSRQRVIFAKSY"
     gene            4953..5255
                     /locus_tag="VIS74_01545"
     CDS             4953..5255
                     /locus_tag="VIS74_01545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5791949.1"
                     /translation="MARSITGNHAVTGVFMLGLGIIAFFELWGPGIMFVIAAAMLVSA
                     LVDGKLGENLMGILILLAIGVIGLLGKLHLEIGFPLGPILFIAIGLGYLVKTFWKR"
     gene            complement(5286..6389)
                     /locus_tag="VIS74_01550"
     CDS             complement(5286..6389)
                     /locus_tag="VIS74_01550"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HEY5791950.1"
                     /translation="MKPQTVVELAQALIRIPSVNPDGETDGSETGEKACAEFVAAFCE
                     SFGGEAALPEVLPGRPNVVVRFPSAAPRKSRILFAPHTDTVSVAGMTVAPFAAELREG
                     KIWGRGASDTKGPMAAMLWALHESRELLAELPHEIWFAGLMGEEAGLHGSKALAAETA
                     FDFVIVGEPTGLRTVNCHKGQVRLTLATAGKACHASQPERGENAIYKMLDLAACVRDE
                     IAPELERQTHPLLGHSTISLGTIRGGSKVNIVPDQCRAQVDIRLVPGFSADAAIRRLR
                     EVCPDVQIDASLSAPLQTSENHPLVQKLVEAGAPCTGAPWFCDAAAFAEKGVPAVAIG
                     PGSIEQAHTADEWIAVSDLEAGVDFFRRFLRSL"
     gene            complement(6386..>6849)
                     /locus_tag="VIS74_01555"
     CDS             complement(6386..>6849)
                     /locus_tag="VIS74_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEY5791951.1"
                     /translation="NFTNLYGMRTVCLRFFTVYGPGQRPDLAIYKFTDSIYRGKPVPK
                     FGDGSTRRDYTYADDIVEGILGALAYPGERFDIFNLGENETTTLNELIAAIESALGKP
                     AIIEQLPEQKGDMPRTCADISKARRLLNYQPQTPISVGIPKFVDWYLSRRK"
BASE COUNT         1304 a         2179 c         1944 g         1422 t
ORIGIN      
        1 gaggcggcgg gaaatgcccc ggagtccagc gatccgcacg gcgaatttcg tggaaaaaac
       61 atcctcatcg aacgcgaatc tggcggcgac ttctccgccg cccgcgaaaa gctccgcctg
      121 gtccgtgagc ggcgcccgcg cccgcatttg gatgacaaaa ttctcaccgc ctggaatgcg
      181 ctcatgattt ccgccttcgc caaggcggga agctccctgg gcgaaccggc ctttctcgcc
      241 accgccacgc gcgcggtgaa tttccttcgg gaaaacctgt ggtcgggtgg cgaactttac
      301 cggtcctggc ggcggggcaa acgctccact cccgcctttg ccgaggacca cgcgtttctc
      361 atttccgccc tgttcgacct ttaccagagc acgttcgatc cggcccacct cacctgggcc
      421 cgcgagctgc aggcccggca ggacgcgctg ttttgggatg acgccatcgg cgcctattac
      481 agcaatcgcg cgggcgactc gcacctcaag gtccggatga aggaggatta cgatggcgcg
      541 gagcccgccg ccaactccgt cagcgcggag aatctgctcc ggctggcccg gatgtttcat
      601 gacgaggaag ccgaggaacg cgcccaatcc attttccgat cgctcgcgcc ggtcctgaac
      661 cgcatcccgc attccgttcc gcagctcctg agcgcctgga tgctggcgcg ggagcccgcg
      721 cggcaaatcg tcatcgccgg cggcctggac gccccggaaa cccgggcatt gttaaagccg
      781 atccgggaaa cctttctgcc ggatgccgtg gtcctgcatt cctcgccgga aatgccggaa
      841 gcgatccggc tcatgcgtcc cgtcagcggc aagccggcgg tttacgtttg cgagaacttt
      901 cgctgcctcg ccccggtcac cgatcccggc cagcttcggc tcacgccgta agcatcgccg
      961 cgccgaacac ccccgcgcta tcgcccagga cgggtttgag cagggcggcc tcgaaggttg
     1021 gattaaaaat ccgttccgcg attttttgcc gggtcgcttc ggtgtaaagc acgggaatat
     1081 tgcccacgcc gccgccaatg acgatcgcgt gcggatcgaa gacatcgatg atctgataaa
     1141 tggctttcgc gaaataatcg ctgagccgtt ccagagtcgc cgcggccacg gcatctgcgc
     1201 ccgcatcggc gcgctcgaca atttccttca gtccccgcct cacgccggac tccccggcat
     1261 aaaatttctc gagagacggg ccgctcaggt aagcttccac cgttccccgc gtgccgtaaa
     1321 cggagaccgg gccgtgggga tccaggacga tctgccccca ttcgccggcg atgccgtggc
     1381 agccattgag caccttgccg cgaaccacca ggccgccgcc cgcgccggtg ccgagaatga
     1441 tgccgaaaac cgtctcgtaa cccttcgccg cacccatcgc cgcctcagcc agcgcgaagc
     1501 aatttgcgtc attcgcaagc tcaaagaccc gccccaagcg cgtttcgagg tcggccttga
     1561 gcggtttgcc gttgaggcat tgggtattgg aacccttgag cacctgcgtg cgcggatcga
     1621 gcgttccggg gtggccgatc ccgatgaccg gcggcaattc cgcccccgcc tcggccacca
     1681 gcgtgcggca ggctttttcg atctggctca ggatgtgatc gtatccccgc tcctgctcgg
     1741 ttggaacgcg cagccggtgc agcggccggt ccggctggcc cgaatccagc aaggcggctt
     1801 ctattttggt tccgccgaga tcaataccga gaaggttctg gggcataagc tgaaaaatcc
     1861 accaggaaaa gcctatttgg attttttccc agtgatctgg ctgggttgat acccagcagg
     1921 cgcttccagc aatcgaagtt gggaatcgtg atgaccgttt cttttcgcca cgccgtgaaa
     1981 actattttcc tcaacgtcct cattccccca ttggctccag ccaggacgtc gaaaacgagc
     2041 gaataattcc aagtaaggcc cggatgaaca tttctcgatg agatcgtaaa tttcgtcggg
     2101 tttgcgggaa tgttcccgct tacgggtgcc aaaaagattt acctgggttc ttcctggatt
     2161 gagtgtccgc atgcttcccc gaaccccaaa aagcacgagc tcagttacgt ttcgaaaata
     2221 aaagccaact ccccgtccgt ccgggccgcc atcttttcga accttatacc agacaaggtt
     2281 gcttttatag gtaaaacccc agcccctcat gacctcaagc ccctccgcca gcaaggcatt
     2341 aggaacccaa agataaagat gcgactgggc cgccgccaga tggccgactg gcaattggat
     2401 gatttcctgc aaatccatag tcggataacg cagcagccgc ttgtgttcag gtgcaacctt
     2461 cccggttcga ttctgaaact gccagggggg gtctgctaaa atcgtcgaat aacgagcctg
     2521 aaccttttcc aacaggtcgt ctgccggcgt gggttcggta aggctggtaa ctttcgacat
     2581 gagattatca aatcacgatg acagaatctg gaattccgat caacacaagc gggcagggat
     2641 ttccgactcc ccgccgaatt cgatcttcca atttgctcca atgcgtggtc gcttccccaa
     2701 atttatcggc cacaaacgca tgggcccagc ctgccgcgaa ttctcctttg gacttcgcgg
     2761 cagtctcgta gagtccggtc tgccgacgag taggctcaag ctcgaatctt gccaaatcct
     2821 gaacgctatc aatttcttca ttcagagcaa attgccgaag gatatctcgc attttttgat
     2881 gaagacttcg ccctcgtgta atgattccgc caaccgaaac cgcaccttcg gcgtgaaggg
     2941 ttttaaagtt ttccaaatcg cgatcgaaaa atggatcctt gttgttccat tcgatttcta
     3001 aagcgaccgt gcctcgcgaa aaattcttaa cgtgatcaat ctcgtggctc ctcgactctt
     3061 tctcaattcc gtcaacggtt cgtttgatca cgaaattatg tttttcccaa cccaactccg
     3121 caagctgccg tcgcaatcgc tgagtaacaa gcgcttctcc tccgcctccc tgaaccagct
     3181 ccaggaccgg aatttgcaat tcaagcaaag ctccttctaa ttcgaccagt gcttggggca
     3241 tatcgtgcaa aaggatcgct tccgcatgat ggaggacctc aacgtcaaag ccccgtttct
     3301 tcagtttctc aaagatcgcg ggcattttgt ttagtaggac ttcgcaaaaa tcacccgctg
     3361 ccggctgggc tcgccggtga agatgcaaac ccccggttcc ggcgattcct cgaagggaat
     3421 gcagcgaatg gtcaccttca ggtcgttttt gatctgctct tccaccgccg cgctgccgtt
     3481 ccagtgcgcg agggcgaacc cgccgtgaat ctcgggcttg tccggattct tcgcggtgaa
     3541 atagtcgtag aacgcttcct tgctcccgag gatcttgaag ttttcgtcgc ggaacgcagt
     3601 ggcgcgatcc agcagcgcct gctggatttc cgtgagaacc gtggcgacgg aagtcacgaa
     3661 atccgcgatc tgcaaaaact gttttgcctt cggcggctgg tcgcggcgcg tcacggccat
     3721 agagcctttt tccagatcgc gcgggccgat ctccacgcgc aacgggatgc ccttcttgac
     3781 ccaatcccaa ttcttcacgc cgccgccggc atcgcgccgg tccacttcca cgcggatcgg
     3841 ctggccgctg aacgaagtgg cgcgcagatc ggcggcaagt ttttcgcacg ccgccagcac
     3901 ggcctcgcgc gtttcctcct tcggcgtaac gggcaggatg accacgtgcg acggcgccac
     3961 cttcggcggg atgatcacgc catcgtcatc gccatgcgtc atgatgagcg tgccgatcaa
     4021 ccgcgtgctc acgccccagc tcgtcgtcca cgcatgctcg attttgcctt cgcgcgactg
     4081 gaattggatg ccggaagccc gggcgaaatt ctggccgagg aaatgagacg tgccggcttg
     4141 aatggctttg cggtcctgca ccatggcctc gatgcaatac gtccgcaccg cgccgggaaa
     4201 acgctcgctc tcggtcttct cgccgcaaat cacgggaacc gcaaggtaat cgcgcgcaaa
     4261 ggtttcgtaa acgcgcagca ttttttcggt ttcctccacc gcctcggctt cggtctcgtg
     4321 cgcggtgtgg ccctcctgcc agaggaattc cgcagtgcgc aaaaacaggc gcgggcgcat
     4381 ctcccagcgg acgacgttcg cccattggtt gatgagaatc ggcaggtcgc gatacgagtt
     4441 cacccacttc gcgtaggtcg cgccgatgat cgtctccgag gtcgggcgca cgatcagcgg
     4501 ttcattgagt tcgccggacg gcaccagctt gccgtccacc agctccagcc ggtggtgcgt
     4561 gacgaccgcg cattccttgg caaatccctc gacgtgctcg gcttccttct gcaggtggct
     4621 caggggaatg aagagcggaa agtaggcgtt cttgtgcccg gtgtccttga acatgccgtc
     4681 gagcaccgcc tgcatgcgtt cccacagcgc gtaaccccac ggtttgatga ccatgcagcc
     4741 gcgaacctcg gaattttccg ccaattccgc cgcgcggacg gcctgttgat accactcggg
     4801 aaaatcctcg tcgcgccggg gggaaatcgc cgttttttcg ttcgaactca taaaatgggg
     4861 tgatctgata tgcggcttca gggcggcggt gtcaatgcgc gggcgcggaa tcccgcttgg
     4921 caatgacttg gcgaagcgat tgaatgcccc gcatggcgcg ttcaatcacg ggcaatcatg
     4981 ccgtcaccgg cgttttcatg ctgggcttgg gaatcattgc cttcttcgag ctgtgggggc
     5041 cgggcattat gttcgtcatc gccgccgcca tgctggtaag cgcgttggtg gatggaaagc
     5101 tcggggaaaa tctcatgggc attctcatcc tgctcgccat cggcgtgatc gggctcctcg
     5161 gcaagctgca tctggaaatc ggctttccgc tcgggccgat tctgtttatc gccatcggcc
     5221 tcggttatct ggtgaagact ttctggaagc ggtaggcaag ggcccgccgc cctccccgtt
     5281 gccgatcaga ggctgcgcag gaaccgccgg aaaaaatcca ctccggcttc gagatcggat
     5341 acagcgatcc attcgtccgc cgtgtgcgct tgttcgatgg aacccggccc gatggccacc
     5401 gcgggcacgc ctttttccgc aaacgccgcc gcgtcgcaga accacggagc gccggtgcag
     5461 ggagcgccgg cctcgacgag tttttggacg agcggatgat tctccgaggt ttgcagcggc
     5521 gccgacaggc tggcatcgat ttgcacgtcc ggacacactt cgcgcaaacg ccggattgcg
     5581 gcatccgcgc tgaaacccgg aaccaggcgg atgtccacct gcgcgcggca ttggtccggc
     5641 acgatgttca ccttcgaccc gccgcgaatg gttcccaggc tgatggtgga gtgcccgagc
     5701 aggggatgcg tctgccgctc gagttcgggc gcgatttcat cccgcacgca ggcggcgaga
     5761 tcgagcatct tatagatcgc gttttccccg cgctccggct gcgaggcgtg gcaggccttg
     5821 cccgcggtgg caagcgtcag ccggacttgc cccttgtggc aattcacagt ccgcaagccc
     5881 gtcggctcgc cgacgatcac aaaatcaaac gccgtctccg ccgccagcgc cttcgaaccg
     5941 tgcaggcccg cctcttcacc catcaagccc gcaaaccaga tttcgtgcgg aagttcggcg
     6001 aggagttccc gggattcatg cagcgcccag agcatggcgg ccatcggccc cttggtatcg
     6061 cttgcgccgc ggccccagat tttgccctcg cgcaattccg cggcgaaggg agccaccgtc
     6121 attccggcca cgctgaccgt gtccgtatgc ggggcaaaaa gaatccggga tttccggggc
     6181 gccgcggaag gaaaccgcac cacgacgttc gggcggccgg gcaggacttc gggcagtgcc
     6241 gcttcccctc caaaggactc gcagaaagcc gccacgaatt ccgcgcaggc cttttcgccg
     6301 gtttcgctgc cgtcggtctc cccgtccgga ttcacgctgg gaatccggat caatgcctgc
     6361 gcgagctcga cgacggtctg cggcttcatt ttcttcggct cagataccaa tccacaaatt
     6421 tcggaatgcc cacggaaatc ggtgtctgcg gctgataatt caacaaccgc cgcgccttgg
     6481 aaatatccgc gcaggttcgc ggcatgtcgc ccttttgctc cggcagctgc tcgatgatcg
     6541 ccggcttgcc cagcgcggat tcgatcgccg cgatcaattc attgagcgtc gtcgtttcat
     6601 tctcaccgag gttgaagatg tcgaaccgct cgcccggata cgccagcgcg ccgaggatgc
     6661 cctccacgat gtcgtcggca tacgtgtaat cgcgccgcgt ggagccgtcg ccgaacttgg
     6721 gaaccggttt gccgcggtaa atggagtccg tgaatttata aatcgccaga tcgggccgct
     6781 ggcccggacc atacacggtg aagaaccgca ggcacacggt ccgcatcccg taaaggttcg
     6841 taaaattac
//
LOCUS       DATDAY010000078         2416 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_1827479, whole genome shotgun sequence.
ACCESSION   DATDAY010000078 DATDAY010000000
VERSION     DATDAY010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2416)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2416)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2416
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1827479"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..660)
                     /locus_tag="VIS74_01560"
     CDS             complement(<1..660)
                     /locus_tag="VIS74_01560"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HEY5791952.1"
                     /translation="MSDKKKLPFWQLLKAWAQPYRMLAGFMYPYRGRFFIGVAFGAGF
                     ALLTASLAPTLYLVARQIFSAAGGAPKLPGFLANLELPEWMSLHPDLAGVVVACILIP
                     SVMLVRSLFSYGNSYFMAWVALRMLSDLRTAVFAHVASQSMDFFNQARSGKLMSRITN
                     DTRVAQNAFTSVAGDLFRDPIAVLGGVVVLFVLDWKFCLAMLVLFPVCLVPIAIFGRK
                     VR"
     gene            complement(679..1761)
                     /locus_tag="VIS74_01565"
     CDS             complement(679..1761)
                     /locus_tag="VIS74_01565"
                     /inference="COORDINATES: protein motif:HMM:NF018460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEY5791953.1"
                     /translation="MNRPEETILAIFAFVLGAIVGSFLNAVIHRLPRSISLHHPRRSF
                     CPSCQRTLPWHENLPLVSYLFLRGKCAGCGARIPVRYWIVEAVTAGIFLWLWLAFRWE
                     LAVVYWIFTALLIAATFIDFEHFIIPDEITWGGVGAGLAASLLVPELMGTASRGFALL
                     NSLIGAAAGYCSLWAVVEAGKICFGRKKHAFAEPVPFRWERHGETAAIEVEDERTEWE
                     EIFSRRSDQLVLECPEFAFDGQPPGSGQLVFFHDRVRSGGVETPLDGLQSITGKVSQL
                     VIPREAMGFGDVKFLAAIGAFLGWPAVFFTIFAGSIAGCVFGVIVLLRAGRGGVRIPF
                     GPYLALGALLWLAGGRELWDWYFQAF"
     gene            complement(1758..2327)
                     /locus_tag="VIS74_01570"
     CDS             complement(1758..2327)
                     /locus_tag="VIS74_01570"
                     /inference="COORDINATES: protein motif:HMM:NF024969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEY5791954.1"
                     /translation="MSKQFEKSLKKLNEALAASAGLGPVVEKAANLVAEALIAGNKLL
                     ICGNGGSGADSSHIATEFTCRFSQDRRPYPAISLTADGGLLLAIGNDYEFQDVFSRQV
                     KAFGKPGDVLIGLSTSGKSRNVLDAIEEARRLGVKSIAFLGKGGGFTTGAADVEIIVP
                     HAETARVQEVQKFLLHVLCEIVEEKLPKE"
BASE COUNT          512 a          775 c          755 g          374 t
ORIGIN      
        1 gcgcaccttg cggccgaaga tggcgatcgg caccaggcag accggaaaga gcacgagcat
       61 cgcgaggcag aatttccaat ccagcacgaa cagcaccacc acgccgccga gcacggcgat
      121 cggatcgcga aacaaatccc ccgcgacgct cgtgaaggcg ttctgcgcga cgcgcgtgtc
      181 gttcgtgatc cgcgacatga gcttgcccga gcgggcctga ttgaagaaat ccatggactg
      241 cgaagccacg tgggcgaaca ccgccgtgcg caggtcgctg agcatccgca gcgccaccca
      301 ggccatgaag taggaattgc cgtaggaaaa caggctgcgg accagcatca ccgaagggat
      361 caggatgcag gcaaccacca cgccggctaa gtccggatgg agcgacatcc attccggcaa
      421 ttcgagattg gcgaggaatc cgggcagctt tggggctccg ccggcggcgc tgaaaatttg
      481 ccgggcgaca agatacaggg tcggcgcgag gctcgccgtg agcagggcaa aacccgcccc
      541 gaacgccacg ccgatgaaaa agcggccgcg gtagggatac atgaatccgg ccagcatgcg
      601 atacggctgg gcccacgctt tgaggagctg ccagaaggga agtttctttt tgtcggacac
      661 aggaagggag aattaacctc agaatgcctg aaaataccag tcccacagct cgcgcccgcc
      721 cgcgagccag agcagggcgc ccagcgccag gtaggggccg aaggggattc gcactccgcc
      781 ccggccggcg cgcagcagga cgatcacccc gaaaacgcag ccggcaatgg accccgcgaa
      841 aatcgtgaaa aacaccgccg gccagccgag aaacgcgccg atggcggcga gaaatttcac
      901 gtccccgaat cccatggcct cgcgggggat cacgagctgg ctgaccttgc cggtgatgct
      961 ttgaagcccg tccagcgggg tttccacgcc gccgctccgc acgcgatcgt ggaaaaacac
     1021 gagctggccc gaacccggcg gctgcccgtc gaacgcgaat tccgggcatt cgagcaccaa
     1081 ttgatcggac cggcggctga agatttcctc ccattccgtg cgctcgtcct cgacctcgat
     1141 cgccgccgtc tccccgtgcc gctcccagcg gaaaggcacc ggctcggcga aggcgtgctt
     1201 tttgcggccg aagcaaattt tccccgcctc caccacggcc cacaagctgc aataaccggc
     1261 cgcggcgccg atcagggagt tcagcagcgc gaacccgcgg ctggcggttc ccatcaactc
     1321 cggcaccagc aggctcgccg ccagcccggc gcccacgccg ccccaggtga tctcatcggg
     1381 aatgatgaaa tgctcaaaat caatgaacgt cgcggcgatg agcagcgcgg taaagatcca
     1441 ataaaccacc gccagctccc agcggaacgc gagccagagc cagagaaaaa tgccggcggt
     1501 gaccgcttcc acgatccagt agcgcacggg aatccgcgcg ccgcagcccg cgcattttcc
     1561 gcgcaggaac agataactca ccagcggcag attttcatgc cacggcagcg tgcgctggca
     1621 ggacgggcag aaggagcggc ggggatgatg cagcgaaatg ctccgcggca gccggtggat
     1681 caccgcgtta aggaaggagc cgacgatcgc tcccagcaca aaggcgaaaa tggcgaggat
     1741 cgtctcctcc gggcggttca ttccttcggt aatttttcct ccacgatctc gcaaagcacg
     1801 tggagcaaaa atttctgcac ttcctgcacc cgggccgttt ccgcatgggg aacaatgatt
     1861 tctacatccg ccgcgcccgt cgtgaagccg ccgcccttgc cgagaaacgc gatggatttg
     1921 accccgaggc gccgcgcttc ctcgatggcg tcgaggacgt tgcgggattt gccgctcgtg
     1981 gaaaggccga tgagcacatc cccgggtttg ccgaaggctt tgacctgccg ggaaaagacg
     2041 tcctgaaatt cgtaatcgtt gccaatggcc agcagcaggc cgccgtcggc cgtcagggag
     2101 atggcgggat acggccggcg gtcctggctg aagcggcagg tgaactccgt ggcgatgtgc
     2161 gaggaatccg cgccgctgcc gccatttccg cagatcagga gtttgtttcc ggcgatcaac
     2221 gcctccgcca cgagattggc ggccttctcc accacgggtc cgaggccggc ggaagccgcg
     2281 agggcttcgt tgagtttttt caggcttttt tcaaattgct tgctcatgca acggggcgga
     2341 ttgggccgac aggcttagtc tcggatcggt cttcagtttc tctgccaggc agggccagcg
     2401 gccgggctcc atcgaa
//
LOCUS       DATDAY010000079         3291 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_2026898, whole genome shotgun sequence.
ACCESSION   DATDAY010000079 DATDAY010000000
VERSION     DATDAY010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 3291)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3291)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3291
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2026898"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1527
                     /locus_tag="VIS74_01575"
     CDS             <1..1527
                     /locus_tag="VIS74_01575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HEY5791955.1"
                     /translation="SIASFLQINDRRIAAEKALAEAKLRTEEAEDARRLAEISAAIAQ
                     ENEEKALSALQAKQAAEKAAEQAKVEAAHAAVQSLQARKETERALAARQSQEEKFRQQ
                     EKMLRQQIEDLNQQRPAEAAAPDEPSPEISEPKLEEARAALTRAASTYSREFSPFDLQ
                     RAQRNPDGVIAGLSRCMDDVSRALMVNPGYAPALMLKGRLHLVFREWDQALSSFQRAK
                     AGIQSRPDSAFGENPDQLLGLIEPLQHASGNSGKNPTQVLVDSPLHDDRVTASLIEFL
                     RKNAGSRAAPSAPVERKVTPGEVALRLVEINPGKSPDVEFYTNELGLAAMRIRNGSAI
                     HDLSPLQVLDLAELDLQGLGRTDWETIYALHPERLTLRECQSGASPAISGRGVAMLKE
                     LRVENSGLTSLEFLRAATQLERLFLNHTQVKDFSPLSSRRLRTLEMDGLMPVSLFSLQ
                     SLPLETLVIGRELAEDSGRIRPLRYHRTLKYIRVPDDPEWQTAPEFWQKVDAGAYTKQ
                     "
     gene            complement(1512..2618)
                     /locus_tag="VIS74_01580"
     CDS             complement(1512..2618)
                     /locus_tag="VIS74_01580"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEY5791956.1"
                     /translation="MPASCKTPVALVRRGFSRSGGAEAYLRRLATGLFEKGCEVTLYN
                     DRPWPEDYWPYPQLSLSQGGGAPREFSRAFHRLRNPQEIIFSMERVVGCDIYRAGDGV
                     HAAWLERRAEHEAPIRTFIRRFTPKHRALLELERQVFHAEHTRHIIANSQMVKREITS
                     RFHYPAERVSVIYNGVPPHKPFTQARERVRENLGFSPSDCVVLFAGSGWERKGLRYAI
                     EACRGFPNLTLLVAGRGDAPARPDFVRYLGEVRGMERYFTAADFFLLPTLYDPFSNAC
                     LEALAAGLPVITTQFNGVSETLEDGVTGSLVADAAFIPELRRAVGFWMRPENRVPAAG
                     RCRALADRLSLEENTRQTLELILRAGAEKNHCFV"
     gene            2732..>3291
                     /locus_tag="VIS74_01585"
     CDS             2732..>3291
                     /locus_tag="VIS74_01585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5791957.1"
                     /translation="MRILVVQLKRLGDLVLTTPVFAALREALPEARITLAAPAAAADL
                     APLLDVDEILLFGNGRADWLRLLRGKFDVCLDFTGNDRSLLVSALSRARMRITYQRFA
                     AKPFRRFVFTRFVDSNVSRRHTADHHVDLLQPLGIHRERVPSHLRAGPLPAGIAKPYA
                     ILHAGTARREKYWSAEGWAGVGRMLA"
BASE COUNT          593 a         1040 c         1099 g          559 t
ORIGIN      
        1 tcgatcgcct cctttctaca gatcaatgac cggcgcatcg ccgcggaaaa ggcgctcgcc
       61 gaggcgaagc tgcgcacgga ggaggcggag gacgcccgcc ggctggcgga aatcagcgcg
      121 gccatcgcgc aggaaaatga ggaaaaggcc ctgagcgcgc ttcaggccaa gcaggcggcg
      181 gagaaggccg ccgagcaggc gaaggtggag gccgcccacg cggcggttca atccctccag
      241 gcccggaagg aaaccgagcg ggccctcgcc gcccgccaga gccaggagga aaaattccgg
      301 cagcaggaaa aaatgctgcg ccagcagatc gaggatctca accagcagcg cccggccgag
      361 gcggccgcgc cggacgagcc gagcccggag atttcggagc cgaaactgga ggaagcccgc
      421 gcggccctga cgcgcgcggc ctcgacttac agccgggagt tttccccgtt tgatctccag
      481 cgcgcccaac gcaatccgga tggagtcatc gcgggacttt cccgctgcat ggatgacgtg
      541 tcgagggcat tgatggtgaa tcccggctac gcgcccgccc tgatgttgaa aggccggctg
      601 cacctggttt tccgggagtg ggatcaggcg ctctcgagct tccagcgcgc gaaggcgggc
      661 attcaatccc gcccggacag cgccttcggg gaaaacccgg atcagttgct cggcctcatc
      721 gaaccgctcc agcacgcctc cgggaattcc ggcaaaaatc cgacgcaggt cttggtcgat
      781 tccccgctgc acgacgaccg ggtgacggcg tcgctcattg aatttttgcg aaaaaatgcc
      841 ggttcgcgcg cggcgccgtc ggctccggtt gagcgaaagg tgactcccgg cgaagtggcc
      901 ctccgactgg tcgagatcaa tccgggcaaa tcgcccgatg tggaatttta caccaacgaa
      961 ctcgggctgg ccgcgatgcg gatccggaat ggttcagcca tccacgatct ctcgccgctg
     1021 caggtgctgg atctcgcgga gctggattta cagggattgg ggcgcacgga ctgggagacg
     1081 atttacgcgc tgcatcccga gcgcctgacg ctgcgcgaat gccaatcggg ggcgtctccc
     1141 gcgatcagcg ggcgcggcgt ggccatgctc aaggaactgc gcgtggagaa cagcggcctc
     1201 accagcctcg agttcttgcg cgccgccacg cagctggagc ggctgtttct gaatcacacc
     1261 caggtgaagg atttttcccc gctttccagc cggcggctgc gaacgctgga gatggatggc
     1321 ctcatgcccg tctccctttt ttcgctgcag tcgctgccgc tggagacgct ggtgatcgga
     1381 cgggagctgg cggaggattc gggccgcatc cgcccgttgc gttaccatcg caccctcaaa
     1441 tacatccgcg ttcccgacga ccccgagtgg cagaccgcgc ccgaattctg gcaaaaggtc
     1501 gatgcgggcg cttacacgaa gcagtgattt ttttccgccc cggcgcgcag gatgagttcc
     1561 agggtctgcc gcgtattttc ctccagcgag agccggtccg ccagcgcgcg gcagcggccg
     1621 gccgcaggaa cgcggttttc gggccgcatc cagaagccga ccgcccgccg gagttccgga
     1681 atgaaagccg cgtcggcaac cagtgagccg gttacgccgt cttccagcgt ttccgagacg
     1741 ccgttgaatt gggtggtgat gaccgggagc cccgccgcca gtgcttccag gcaggcgttg
     1801 ctgaagggat cgtaaagcgt ggggagaagg aaaaagtccg cggcggtgaa atagcgttcc
     1861 attccgcgga cttccccgag gtagcggaca aaatccgggc gcgccggcgc atccccgcgt
     1921 ccggccacga gcagcgtgag gttgggaaat ccccggcagg cctcgatggc ataacgcaat
     1981 cccttgcgtt cccagccgct gccggcgaag aggaccacgc aatccgaagg gctgaatccg
     2041 agattttcgc ggacgcgttc gcgcgcctgg gtgaagggtt tgtgcggcgg gacgccattg
     2101 taaatgacgc tgacccgctc cgcgggatag tggaaacgcg aggtgatctc gcgtttaacc
     2161 atctgcgaat tggcgatgat gtggcgggta tgctccgcgt ggaaaacctg ccgctcgagt
     2221 tccagcagcg cgcggtgctt gggggtgaat ctccggatga aggtgcggat cggcgcttcg
     2281 tgttccgccc ggcgctccag ccaggcggcg tgaacgccat ccccggcgcg gtagatgtcg
     2341 caccccacca cgcgttccat gctgaagatg atttcctggg ggttgcgcag ccgatggaaa
     2401 gcgcgggaaa attcgcgcgg ggcgccgccg ccctgggaga gcgagagctg gggatacggc
     2461 cagtaatcct ccggccaggg ccggtcattg taaagcgtga cctcgcatcc cttctcaaaa
     2521 agcccggtgg ccagccgccg cagatacgcc tccgcgcctc cggaacgcga aaacccgcga
     2581 cgcacaaggg ccaccggagt tttgcaggac gcgggcatgg ggtcttggga atttgattgg
     2641 agcatttcag atgtttttgt atcacgctag cccgaatggt catgcctcgt gagttttgct
     2701 cgaaaaggac cggcgtccgg ccggatttcc gatgagaatt ctggtggtcc aactcaaacg
     2761 gctgggagac ctggtgctga ccacgccggt gtttgccgcg ctgcgcgaag ccctgccgga
     2821 ggcgcggatc accctggccg cccccgccgc cgcggcggat ctggcgccgc tgctcgatgt
     2881 ggacgaaatt cttttgtttg gcaatggccg cgcggactgg ctgcggctgc tgcggggaaa
     2941 attcgatgtc tgcctcgact tcacgggaaa tgaccggtcg ctgctcgtct ccgccctgtc
     3001 gcgcgcgcgg atgcggatca cctaccaacg ctttgccgcc aagccgtttc ggcggttcgt
     3061 attcacccgc ttcgtggatt ccaatgtcag ccgccgccac accgcggacc atcacgtgga
     3121 cctgctgcaa cccctgggca tccaccggga aagggtgcca tcccacctgc gcgccgggcc
     3181 gctgccggcc gggatcgcga aaccttacgc cattctccac gccggcaccg cccgccggga
     3241 gaaatattgg agcgcggagg ggtgggccgg ggtgggccgg atgctggcga g
//
LOCUS       DATDAY010000080         3606 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_488189, whole genome shotgun sequence.
ACCESSION   DATDAY010000080 DATDAY010000000
VERSION     DATDAY010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 3606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3606
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_488189"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..415)
                     /locus_tag="VIS74_01590"
     CDS             complement(<1..415)
                     /locus_tag="VIS74_01590"
                     /inference="COORDINATES: protein motif:HMM:NF019977.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase N-terminal domain-containing
                     protein"
                     /protein_id="HEY5791958.1"
                     /translation="MVFEILHTTDYKYSAKASEAYIETRLTPPLLAHQKVQSHRIEFV
                     PDAPTSFYVDYFGNKVHFYSMTLRHERLTITNRLRVETRAAHLPDEALDVPLADARQI
                     INCSLTDVFDYLQGTDAIPTGGMAKPWVRRFLKNGV"
     gene            complement(405..2972)
                     /locus_tag="VIS74_01595"
     CDS             complement(405..2972)
                     /locus_tag="VIS74_01595"
                     /inference="COORDINATES: protein motif:HMM:NF025761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HEY5791959.1"
                     /translation="MQILTSKRAASLAAPSQTWNEVFAAPGTPRLPYAPLVGHFQGLS
                     RTALRKLDDNLESTLREMGVTFDLTRGKPTSQRPWYCDLLPQIFSAPEWDHVTRGMRQ
                     RLNAFELFLRDIYGGRHILKDGKIPVQAVLSSPYFQRAAVGLPLPDNAFLHLCGLSLC
                     RTPDGELHVKHHYFSNASGVSYMMQNRRALARVIPNHFEGYSIHSIVDTPMEILSLLR
                     NFSASSDPTVVLLSPGPGSAAFSEHSFLARRMGISLVQGNDLVVVNDLVYLKTISGLE
                     RVHVIYTRVADAWLDPLAFRPDSYLGVPGLVNSIRKGAVRVINAIGAQAADDRALLCF
                     SQAIIAYYLGEKPILPTLQTYWLGDIDQREFVLGNLEKYTIRPLYGERILTPGQGEPL
                     SDYRRRQVLKEVQQYASAYVAQPRDADAATLSFQSGRRSLRTQDHIAFALRHGEADWE
                     VFPGALTRITTAQSPYTASELGGGSKDTWIESLFGETDAPGEARRIADPRLPAQHVTS
                     RVAEAMYWSGRYLERASSLANMIGVIETLELEELNETERQLYRPIWNRMLPPLETRAE
                     TRRTLSSSRGRYRLALDPRESGSLINNVFRAAGNAESVLECLSSEAWSVLSTLRSRFK
                     AVRFQEKLSEEKKRFATRRMCDAATHLIPQFFGTAEFTMVADGGWNFCLIGQMVERAT
                     VTANAANSMANALLSATGQNARSDHAIEIQLSAFLRLLSCRDAYHRVFQSRIELAPVL
                     ELLWEGEAVPRSVQRCLDRVSTLLQESQPPGSRAIQRTLTEIHSLNSIIRHTSWEKLV
                     EETFGAPAQTAGTKAGLIRHITKLADHVYGIHHYIADGFLNHQIHMHEENQPPLHGL"
     gene            complement(3006..>3606)
                     /locus_tag="VIS74_01600"
     CDS             complement(3006..>3606)
                     /locus_tag="VIS74_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY5791960.1"
                     /translation="ALLTRFYEFDAGRILIDGRDLRDYPLRQLRESIAMVTQESFLFN
                     GTTAENLRVGKAAATEAEMWEVLEAANAAEFVHRLPKGLHTPVGEKGIRLSVGERQRI
                     SIARALLKNPPILILDEATASVDNATERLIQEALDRLLAHRTSFVIAHRLSTIVKADQ
                     ILVLERGRIVERGRHGELLARDGLYARLNRQSLLGFHLT"
BASE COUNT          624 a         1064 c         1263 g          655 t
ORIGIN      
        1 ggacgccatt tttgagaaag cggcgcaccc agggtttggc catgccgccg gtcggaatgg
       61 catccgtgcc ctggaggtaa tcgaacacgt cggtcagcga gcaattgatg atctggcggg
      121 catcggcgag cggcacgtcg agcgcctcgt ccggcaaatg cgcggcgcgg gtctcgacgc
      181 gcaggcggtt ggtgatcgtc aggcgctcgt gccgcagcgt catgctgtag aagtgcacct
      241 tgttgccgaa ataatccacg tagaacgagg tgggcgcatc cggcacgaac tcgatgcggt
      301 gggactgcac cttctggtgg gcgagcagcg gcggagtcag ccgcgtctcg atgtaggcct
      361 cgctcgcctt ggcgctgtat ttgtaatccg tcgtatggag aatctcaaag accatggagc
      421 ggcggctggt tttcctcgtg catgtggatt tggtgattca gaaacccatc ggcgatgtag
      481 tgatggatgc catacacgtg atcggcgagc ttggtgatgt ggcggatcaa accggccttc
      541 gtgcccgcgg tctgcgcggg ggcgccgaag gtttcctcca ccagcttttc ccaggaggta
      601 tggcggatga tgctgttcag cgaatgaatc tcggtcagcg tgcgctggat ggcgcgcgag
      661 cccggcggct gggattcctg cagcagcgtg gaaacgcggt ccaggcagcg ctgcacggag
      721 cgcggcacgg cttcgccctc ccagagcagt tccagcaccg gggcgagctc gatgcggctc
      781 tggaagacgc ggtgatacgc gtcgcggcag ctgagcaggc ggagaaaggc cgagagctgg
      841 atttcaatgg cgtgatcgct ccgcgcgttc tgccccgtgg cggaaagcag ggcgttggcc
      901 atggaattgg ccgcgttggc cgtgaccgtg gcgcgttcga ccatctggcc gatgaggcag
      961 aagttccagc cgccgtcggc aaccatcgtg aactccgcgg tgccgaaaaa ttgcgggatc
     1021 aggtgcgttg cggcatcgca catccgccgg gtggcgaagc gttttttctc ttcggaaagc
     1081 ttttcctgga aacggacggc cttgaaccgc gagcgcagcg tggagagcac gctccaggcc
     1141 tccgagctca ggcattcgag cacgctctcg gcattgccgg cggcgcggaa tacattgttg
     1201 atcagcgaac cggattcccg cgggtcgagc gcgaggcggt agcggccgcg ggagctcgac
     1261 agcgtgcggc gcgtctcggc gcgggtttcc aacggcggca gcatgcggtt ccagatcggg
     1321 cggtaaagct gccgctcggt ttcattcagc tcttcgagtt cgagggtctc aatgacgccg
     1381 atcatgttgg ccaggctgga ggcgcgttcg aggtagcggc cgctccaata catcgcctcc
     1441 gccacgcggc tggtcacgtg ctgggcgggc agacgcggat cggcaatgcg gcgggcttcg
     1501 ccgggcgcgt cggtctcgcc aaataaactt tcaatccagg tgtccttgct gccgccgccg
     1561 agctccgagg ccgtgtaggg ggattgcgcc gtggtgatgc gggtcagcgc gccgggaaag
     1621 acctcccaat ccgcctcgcc gtggcgcagg gcgaaggcga tgtgatcctg ggtgcgcagg
     1681 gagcggcggc cgctttggaa ggagagcgtg gccgcatccg cgtcgcgcgg ctgggccacg
     1741 taggcggagg cgtattgctg cacctccttg agcacctgcc ggcggcggta gtcgctgagc
     1801 ggctcgcctt gcccgggcgt gaggatgcgt tcgccgtaga gcggacggat cgtgtatttc
     1861 tccaggttgc cgagcacgaa ttcccgctgg tcgatgtcgc cgagccagta ggtttgaagc
     1921 gtgggcagga tgggtttttc accgaggtaa taggcgatga tcgcctggct gaaacacagc
     1981 agcgcgcggt cgtcggcggc ctgcgcgccg atggcgttga tgacgcgcac ggcgcccttg
     2041 cggatcgaat tcaccagccc cgggacgccc aggtaggagt cgggccggaa cgccagcggg
     2101 tccagccacg catcggccac gcgcgtgtag atgacatgga cgcgctccag cccggaaatg
     2161 gttttaaggt aaaccaaatc gttgacgacc acgagatcgt tcccctgcac gagcgaaatg
     2221 cccatgcggc gcgcgagaaa actgtgttcg gaaaacgccg cgcttcccgg ccccggggaa
     2281 agcagcacca ccgtgggatc cgagctcgcg gaaaaattcc gcagcaggga aagaatttcc
     2341 atgggcgtgt ccacgatgga gtgaatggag taaccctcga aatgattcgg gatcacccgg
     2401 gccagcgcgc gccggttttg catcatgtag gacacgccgg acgcgttgct gaagtagtgg
     2461 tgcttgacgt gcagttcgcc gtccggcgtg cggcacaggc tcaggccgca gaggtgcaga
     2521 aaggcgttgt cgggaagcgg caggccgacc gccgcgcgct ggaaataggg gctcgaaagc
     2581 accgcctgca cggggatttt gccatccttc aaaatgtggc ggccgccgta gatgtcgcgc
     2641 aggaaaagct cgaaggcgtt caaccgctgg cgcatgccgc gggtgacgtg gtcccattcc
     2701 ggcgcgctga aaatttgcgg cagcagatcg caataccagg gccgctggct cgtgggtttg
     2761 ccgcgcgtca gatcgaaggt gacgcccatt tcccggagcg tggactcgag gttgtcgtcg
     2821 agtttgcgca gcgccgtgcg ggaaagtccc tgaaagtggc cgaccaacgg cgcgtacggc
     2881 agccgcggcg tgcccggcgc ggcgaagact tcattccagg tctgggatgg agccgcgagg
     2941 gatgccgcgc gtttgcttgt caggatttgc acgcttcgag ccttggcgga gcggggcggg
     3001 agaggtcaag ttaaatggaa ccccagcagc gactgccggt tgagccgcgc gtaaagcccg
     3061 tcccgcgcca gcagctcccc gtggcggccc cgctccacga tccggccgcg ctccagcacc
     3121 aggatctggt cggctttgac gatggtggaa agccggtgcg cgatgacaaa actcgtccgg
     3181 tgcgccagca ggcggtcgag cgcttcctga atcagccgct ccgtggcgtt gtccacgctc
     3241 gcggtggctt cgtcgaggat caggatcggc ggatttttta acagcgcccg cgcgatcgag
     3301 atgcgctggc gttcgcccac gctcaggcgg attccctttt cccccaccgg ggtgtgcaat
     3361 cccttcggca gccggtggac gaattccgcg gcgttggccg cttccagcac ctcccacatt
     3421 tccgcctcgg tggcggcggc tttgccgacc cggaggtttt cggcggtggt tccattaaaa
     3481 agaaaacttt cctgcgtgac catggcgatg gattcccgga gttgcctcag cgggtagtcc
     3541 cgcaaatccc ggccatcgat gagaatccgg ccggcgtcaa attcgtagaa ccgcgtgagc
     3601 agggcc
//
LOCUS       DATDAY010000081         3333 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_766341, whole genome shotgun sequence.
ACCESSION   DATDAY010000081 DATDAY010000000
VERSION     DATDAY010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 3333)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3333)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3333
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_766341"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..234)
                     /locus_tag="VIS74_01605"
     CDS             complement(<1..234)
                     /locus_tag="VIS74_01605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5791961.1"
                     /translation="MSTAELTTPATAEWKLPSQRKVGMICLITTESALFSIFVVAYLF
                     YIGKSLNGPYPHEVLHFPWWGSAALFSSSFTIIM"
     gene            complement(231..1940)
                     /gene="ctaD"
                     /locus_tag="VIS74_01610"
     CDS             complement(231..1940)
                     /gene="ctaD"
                     /locus_tag="VIS74_01610"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HEY5791962.1"
                     /translation="MSAHGTTTTADQEPYEFPPLERPGEEKGSLTAIIQNLIFTVDHK
                     KLGLMYISAGLAFFVVAGIMASLIRIQLFWPQSTFLGPDVFNRFFTMHGTAMVFLVGM
                     PILFGFANYLVPIMIGTRDMAFPRLNAFGFWVFLFSGLFLFFSYLGAPGLYDAGSAPD
                     VGWFAYSPLTAKPFSRGHSTDYWTLSILMNGIGSIATAVNILVTVFCLRCKGMTLGRM
                     PLFVWMFVVVAFLVIIAMPPLSAAQIMLTLDRYLDAKFFDTQAGGSAVLWQHFFWIFG
                     HPEVYILIIPGFAIASEVIPVFSRKPIFGYPIMVAATLAIAFISLGVWAHHMFTVGMT
                     SLGNTFFALSTMVIAVPTGIKIFNWIGTMYGGKIRLELPMLFCIAFLFQFLIAGLTGI
                     MLSIVPFDWQLSDSYFVVAHFHYVLIGALLYTFFAGFYYWYPKAFGKMLDVKLGHWHF
                     WLFTIGFHVTFFPQHISGFLGMPRRIFTYEADRGWEIWNQISTIGVIFQVVGVSFFVY
                     NLIYSYFKGKEAGDDPWDAWTLEWATTSPPPEYNFDEIPEVRSRRPLWDKKHPEDPDW
                     KFE"
     gene            complement(1943..3082)
                     /gene="coxB"
                     /locus_tag="VIS74_01615"
     CDS             complement(1943..3082)
                     /gene="coxB"
                     /locus_tag="VIS74_01615"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HEY5791963.1"
                     /translation="MFTCKRALHSGKRAWLDAKWHKLSALLLVFLIVGAHYAVSAEPT
                     LSESEKTQHRIADMFQPLSKPAEMIHDVSLVTLAVCAIIFVIVTGMLAYVVIKFRKPA
                     NDDGQEPPQIYGSRNIELAWTVIPILITIVLAFVTTRTIGEIQDAVMPENALRVRVVG
                     HQWWWEVHYYNEVDGKKISYLTTANEIHVPVSGKDPAKRIPTHITLQSADVIHSFWVP
                     QLAGKIDLVPNRDNEMWIEPYATGTFFGNCAEYCGTQHANMLLRVIVQTPEEFAAWKA
                     EQLAPNAAPQTAQEVRGHQLYFDYSCVNCHKIEDSESIGVFGPDLNKMFTRQTLGAGV
                     APMDEKHLKIWVKDPQVLKVGCLMPDMQLLDNEVNDIVAYLKTLK"
BASE COUNT          752 a          997 c          972 g          612 t
ORIGIN      
        1 catgatgatg gtgaaactgc tgctgaagag cgccgcggag ccccaccagg ggaaatgcag
       61 cacctcatgc gggtaagggc cgttgaggct cttcccgatg taaaacaggt aggcgacgac
      121 aaagatgctg aagagcgccg attccgtcgt gatcaggcag atcatgccca ccttccgttg
      181 ggaaggcagc ttccactcag cggtggcagg ggtcgttagt tcagcggtgc tcattcaaat
      241 ttccagtcag gatcttccgg atgtttcttg tcccacagcg ggcgccggct gcgaacctcg
      301 gggatttcat cgaaattgta ttccggaggc ggcgacgtgg tggcccactc cagcgtccag
      361 gcgtcccacg ggtcatcgcc ggcttccttg cccttgaagt aggagtagat caggttatag
      421 acgaagaagg aaacccccac gacctggaag atcacgccaa tggtcgaaat ctgattccag
      481 atttcccagc cgcgatccgc ctcgtaggtg aaaatgcggc gcggcattcc gaggaatccc
      541 gaaatgtgct gcgggaagaa cgtgacgtgg aagccgatcg tgaaaagcca gaagtgccag
      601 tggcccagct tcacgtcgag catcttgccg aaggccttcg gataccagta gtaaaacccg
      661 gcgaagaagg tgtaaagcag cgcgccgata agcacgtagt ggaagtgcgc caccacgaaa
      721 tacgagtcgc tcagctgcca gtcaaagggc acgatcgaga gcatgatgcc ggtcagcccg
      781 gcgatcaaaa actggaaaag aaacgcgatg cagaacagca tcggcaattc caggcggatt
      841 ttcccgccat acatcgtgcc gatccagttg aagatcttga tgcccgtcgg caccgcgatg
      901 accatcgtgg acaaggcgaa gaaggtattg ccgagcgagg tcatgcccac cgtgaacatg
      961 tggtgcgccc agactcccag gctgatgaag gcgatcgcca gggtcgccgc gaccatgatc
     1021 gggtaaccga agatcggctt gcgcgaaaag accggaatca cctccgaggc gatcgcaaag
     1081 cccggaatga tgaggatata cacctcggga tggccgaaaa tccagaagaa gtgctgccac
     1141 agcaccgccg agccgccggc ctgcgtgtcg aagaacttcg catccagata ccggtccagc
     1201 gtcagcatga tctgggcggc ggaaagcggc ggcatggcga tgatgaccag gaaggccacc
     1261 acgacgaaca tccagacaaa cagcggcatc cggccgagtg tcatgccctt gcagcgcagg
     1321 cagaagacgg tcacgagaat gttcaccgcc gtcgcaatgc tgccgatgcc attcatgaga
     1381 atggaaagcg tccaatagtc cgtgctgtgc ccgcgggaga agggtttggc cgtcagcgga
     1441 gaatacgcaa accagcccac gtccggcgcg gaacccgcgt catacaagcc cggcgctccc
     1501 aggtagctga aaaacaaaaa caggccgctg aagagaaaca cccagaaccc aaaggcgttc
     1561 aggcgcggga atgccatgtc ccgcgtgccg atcatgatcg gcaccaggta gttcgcaaag
     1621 ccgaacagga tgggcatgcc caccaggaac accatggccg tgccgtgcat ggtgaagaaa
     1681 cgattaaaga catccggtcc caggaacgtg ctctgcggcc agaaaagctg aatgcggatc
     1741 agcgaggcca tgatgccggc cacgacaaaa aacgccagac cggcgctgat atacatcagc
     1801 ccgagcttct tgtgatcaac cgtaaaaatc aggttctgga tgatcgccgt cagcgagccc
     1861 ttttcctcgc cgggacgctc cagcggcggg aactcgtagg gttcctggtc ggcggtggtg
     1921 gtggttccgt gtgcggacat ggttacttca gggttttcag gtaggccaca atgtcgttca
     1981 cttcattgtc gagcaattgc atatccggca tcaggcagcc gaccttcaag acctgcgggt
     2041 cctttaccca gattttcagg tgcttttcat ccatgggagc aactcccgcg ccaagcgttt
     2101 gccgggtgaa catcttgttg aggtccgggc cgaatacgcc gatggattcg gaatcctcga
     2161 tcttgtggca attgacgcac gaataatcga agtaaagctg atgcccgcgc acctcctgcg
     2221 ccgtctgcgg cgcggcgttc ggcgccagct gctcggcctt ccacgccgcg aattcctcgg
     2281 gagtctggac aatgacgcgc agcagcatgt tcgcgtgctg cgtgccgcaa tattccgcgc
     2341 aatttccgaa aaatgtgccc gtcgcgtagg gctcgatcca catctcgttg tcgcggttgg
     2401 gcaccaagtc gatcttgccc gccagctgcg gcacccaaaa gctgtgaatg acgtccgcgc
     2461 tttgcagggt gatgtgggtc ggaatccgct tggcgggatc cttgccgctc accggcacgt
     2521 gaatctcatt cgccgtggtg agataactta tcttcttgcc gtccacttca ttgtaataat
     2581 ggacctccca ccaccactgg tggccgacca cgcggacgcg cagcgcattt tccggcatca
     2641 ccgcgtcctg gatttcaccg atggtccgcg tcgtcacaaa cgccagcacg atcgtgatga
     2701 ggatgggaat gaccgtccac gccagctcga tgttccggct gccgtaaatc tgcggcggct
     2761 cctggccgtc gtcattggcc ggcttgcgga acttgatgac cacgtaggcc agcatgcccg
     2821 tcacgataac gaaaataatg gcgcaaaccg ccagcgtgac cagcgaaacg tcatgaatca
     2881 tctccgccgg cttggacagc ggctggaaca tgtcggcgat gcggtgctgg gtcttttccg
     2941 attccgaaag cgtcggttcc gccgagacgg cataatgggc gccaactata agaaaaacta
     3001 ataacagcgc cgaaagcttg tgccatttgg catcaagcca tgcgcgtttt ccagagtgta
     3061 gggcccgctt gcaggtgaac atttttcagc gcccgagttt gcatagcccc acgcgggctg
     3121 caaccctttt tctcagcaat ttttccggca aaaacgatcc cgcgccttcc gggcgggaac
     3181 cccggcaacc cgcgcgcccg gcaccggctg ccctctgatg gcggactccc gcatccccgc
     3241 cccgttcggg tccacggcgg caaaatccag ccaccacggt gcggcaaaac gccgcacctc
     3301 attccctccc cggcaggctt accccacccg cgc
//
LOCUS       DATDAY010000082         2635 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_2001656, whole genome shotgun sequence.
ACCESSION   DATDAY010000082 DATDAY010000000
VERSION     DATDAY010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2635)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2635)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2635
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2001656"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..492
                     /locus_tag="VIS74_01620"
     CDS             <1..492
                     /locus_tag="VIS74_01620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5791964.1"
                     /translation="EFYDPTVPWTDRCPLPVLAGVVWFGFAALTSLAVAIFGPAVLPV
                     FGVFVIGWPAQLLIGASALLWIYGAGAFYRLDRKGWWVIAIGLLVFVVSSAITYACAD
                     ILEIYPKLGYSPEQVAQIAKFNFVKGALGVGFVVLWAVPVFGYLFYVRKFFPGIRPEA
                     AGS"
     gene            complement(570..2081)
                     /locus_tag="VIS74_01625"
     CDS             complement(570..2081)
                     /locus_tag="VIS74_01625"
                     /inference="COORDINATES: protein motif:HMM:NF012457.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HEY5791965.1"
                     /translation="MNEAFLWGVATSGYQSEGGYNQPGQPHNNWAEWEQRGRVQRTGG
                     GPRFWARYAEDFALARGLGLNAFRLGIEWARVQPSFDTDTIPAPDWDESALDGYAQRL
                     AGCRAAGMEPIVTLHHFTHPAWLGIDAWLLPPTVDYFEAYVRHTVREINRRLVERHGQ
                     PPLRTFITLNEPNMLVLNSYFGHQFPAKAMSGSHAMLRAYNHLLAAHVRAYNAIHDLY
                     EEAGWERPRVSLNTFCSDLYWNDKFIWDLLASRELGIGFDQLGRFLLRGETQLQRAVG
                     NADLPFNRNLIWHLGNAVKFLAARLGRRQVIPENFETFLHELARSKRARLFDFIGLDY
                     YDPFFGHLFRLPTFHDLEFPSHGLRDWLMDGMSAKWWNWRCLPQGLPLFARFYSETLF
                     NRPVLIAENGMAMRRKFDNQIVSAREDGLTRSDFLRAHVREVMHAVKEGVPIIGYLHW
                     SLTDNYEWGSFTPRFGLYSLDYAAEAERKDRDQHDDCPSATYRELIRHARGLG"
     gene            complement(2074..>2635)
                     /locus_tag="VIS74_01630"
     CDS             complement(2074..>2635)
                     /locus_tag="VIS74_01630"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016027.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY5791966.1"
                     /translation="IVLVSAGTLGLSPATSVLKRLLQMPEEFQTVVVCGKNHEMRADV
                     EKLAAGSSKKIVTIGYTTEMPALMKTATILLSKPGGLITAEALACGLPMVVLDPIGGQ
                     EERNADVLLEKGAAIKCTEVTLIPYKLGQLLGDPRRVAAMAENARTLGFPRAAKTIVE
                     AVMKDHRLPFIIDAKRKKALQKRANE"
BASE COUNT          444 a          788 c          897 g          506 t
ORIGIN      
        1 gaattttatg atcccaccgt gccctggacc gaccgctgcc cgctgcccgt gctggccgga
       61 gtggtgtggt tcgggttcgc cgccctgacg agcctggcgg tggcgatttt cggacccgcg
      121 gtgctgccgg tcttcggagt ctttgtgatt ggctggccgg cccagctgct catcggcgcg
      181 agcgcgctgc tctggattta cggcgccggg gccttctacc ggctcgaccg gaagggatgg
      241 tgggtgatcg ccattggcct gctggtgttt gtggtttcct cggccatcac gtatgcctgc
      301 gcggacatcc tggagattta tcccaagctc gggtattcgc cggagcaggt cgcgcagatc
      361 gcgaagttca acttcgtgaa gggcgccctc ggcgtcggat tcgtggtgct gtgggccgtg
      421 ccggtattcg gctatctgtt ctacgtccgg aagttttttc cgggaattcg cccggaagcc
      481 gcgggttcgt aatcccgcgc cagccccttc gattcagctt gccgggtgcg cggcggggtt
      541 gccaacgccg ccggggaaag cgcgtcgaat tagcccaaac cgcgggcgtg gcggatgagc
      601 tcgcgatacg tggcggaggg gcagtcgtcg tgctgatccc ggtccttgcg ttccgcttcg
      661 gcggcgtagt ccagcgaata gagcccgaaa cgcggggtga aggagcccca ttcgtaattg
      721 tcggtcagcg accagtgcag gtagccaatg atcggcacgc cttccttgac cgcgtgcatc
      781 acctcccgca cgtgcgcgcg caaaaaatcg ctgcgcgtca ggccatcctc gcgcgcggaa
      841 acgatctggt tgtcgaactt ccgccgcatg gccatgccat tttccgcgat gagcacgggg
      901 cggttgaaga gcgtttcgga ataaaagcgc gcaaacaggg gcagcccctg cggcaggcag
      961 cgccagttcc accatttggc gctcatgcca tccatcagcc agtcgcgcaa tccgtgggag
     1021 gggaattcca gatcgtgaaa cgtgggcagc cggaagagat ggccgaagaa gggatcgtaa
     1081 taatccaggc cgatgaagtc gaagagccgc gcgcgtttcg agcgggcgag ctcgtggagg
     1141 aaagtctcga aattttccgg aatcacctgc cgcctcccca ggcgggcggc caggaatttg
     1201 acggcattgc cgagatgcca gatcaggttg cggttgaagg gcagatcggc gttgcccacc
     1261 gcgcgctgca gctgggtttc cccccgcaac agaaagcgcc ccagttgatc gaagccgatg
     1321 cccaactccc gggaggcgag cagatcccag atgaatttgt cgttccaata aagatcgctg
     1381 caaaaggtgt ttaacgaaac ccgcgggcgc tcccagccgg cttcctcgta gaggtcgtga
     1441 atggcgttgt aggcgcggac atgcgcggcg agcaggtgat tgtaggcgcg cagcatggcg
     1501 tggctgccgg acatcgcctt cgcggggaac tgatggccga agtagctgtt gagcaccagc
     1561 atgtttggct cgttcagggt aatgaacgtg cgcagcggcg gctggccatg gcgctcaacc
     1621 aggcggcggt tgatctcccg gaccgtgtgc cggacgtagg cttcaaaata gtccacggtg
     1681 ggcggcagca gccaggcatc gatgcccagc caggcgggat gcgtaaagtg gtgcagcgtg
     1741 acgattggct ccatgccggc cgcgcggcag ccggccaggc gctgggcgta accgtcgagc
     1801 gcggattcat cccagtcggg cgcggggatg gtgtcggtat cgaacgaagg ctggacgcgg
     1861 gcccattcga tgcccaggcg gaaggcattc aggcccaggc cgcgggcgag ggcgaaatcc
     1921 tccgcgtagc gcgcccagaa ccgcgggccg ccgccggtgc gctgcacgcg gccgcgctgt
     1981 tcccattccg cccaattgtt gtgcggctgg ccgggttggt tgtagccgcc ctcgctctgg
     2041 tagccggacg tggcgacccc ccaaagaaag gcttcattca ttggcgcgct tctggagggc
     2101 ttttttgcgt ttggcgtcaa tgatgaacgg caggcggtgg tccttcatca cggcttccac
     2161 aatggttttc gcggcgcggg gaaatcccag cgtccgcgcg ttttccgcca tggccgccac
     2221 gcgccgggga tcgcccagga gctggccgag cttgtaggga atgagcgtga cctcggtgca
     2281 cttgatggcg gcgccctttt cgagcaggac atcggcattg cgctcctcct gcccgccaat
     2341 ggggtccaat acgaccatcg gcaatccgca cgccagcgcc tcggcggtga tgagtccgcc
     2401 gggtttgctg agcaggatcg tcgcggtttt catgagcgcg ggcatctcgg tggtgtaacc
     2461 aatggttacg attttcttgg acgaacccgc ggcgagtttt tcgacatccg cgcgcatctc
     2521 atggttcttg ccgcagacca cgacggtttg aaactcctcc ggcatctgca gcaggcgctt
     2581 gagcaccgag gtggccgggc tcaggcccag cgtgccggcg gaaacgagca cgatg
//
LOCUS       DATDAY010000083         3351 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_693896, whole genome shotgun sequence.
ACCESSION   DATDAY010000083 DATDAY010000000
VERSION     DATDAY010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 3351)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3351)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3351
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_693896"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1047
                     /gene="mmsA"
                     /locus_tag="VIS74_01635"
     CDS             <1..1047
                     /gene="mmsA"
                     /locus_tag="VIS74_01635"
                     /EC_number="1.2.1.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR01722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004491 - methylmalonate-semialdehyde
                     dehydrogenase (acylating, NAD) activity [Evidence IEA];
                     GO_process: GO:0006574 - valine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEY5791967.1"
                     /translation="PFNFPAMVPMWMFPLALACGNAFVLKPSERDPSASLLLAELLRE
                     AGLPDGVFNVVQGDKAAVDALIEHPGVDAISFVGSTPIAEYIYTESSRRGKRVQALGG
                     AKNHLVVMPDADLDQAASALLGAAYGSAGERCMAISVAVAVGPAGDALIEKLIPLVKG
                     LRIGNGLEGDPDMGPVVTAAHKARIEGYLEEGVKSGAKLLVDGRGLRVAGYENGFFLG
                     GCLFDGVTPEMKIYREEIFGPVLGVVRVPDLESALELINAHEFGNGVSCYTRSGGVAR
                     EFSRRVKIGMVGINVPIPVPMAWHSFGGWKRSLFGGQHIYGEEGVRFYTRYKSIMQRW
                     PAEDHGASFGMPTN"
     gene            complement(1044..1496)
                     /locus_tag="VIS74_01640"
     CDS             complement(1044..1496)
                     /locus_tag="VIS74_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VapC toxin family PIN domain ribonuclease"
                     /protein_id="HEY5791968.1"
                     /translation="MTSIDANLLLFSYSTDSPFHEKARRFLQSIGQREDIGISEFVLA
                     EFYLHLRNPAVLVRPLSASEAAEVIQSYRRHPRWQTFGFPPHSRKLHDTLWHDAAKPN
                     FARRRIYDSRTALTLRAFGVTHFATANVKDFQGFGFERVWNPLIDSKN"
     gene            complement(1493..1750)
                     /locus_tag="VIS74_01645"
     CDS             complement(1493..1750)
                     /locus_tag="VIS74_01645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5791969.1"
                     /translation="MVKTQIQLPEELYQELRSFAKNREWSLAETFRRGAEMLLEVYPA
                     HPVSAAWEPPRSQRVGWKGLSAEQLHDLALADQEGPLPSSR"
     gene            complement(1789..2928)
                     /locus_tag="VIS74_01650"
     CDS             complement(1789..2928)
                     /locus_tag="VIS74_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY5791970.1"
                     /translation="MIPLSFNLARFAAIIRKEFIQMRRDRLTFAMMFGIPVIQLILFG
                     FAINTDPKSLPVAVLAADNSPFARRLAHAMETSGYFKITRHIDTEEAARQLLARGEVQ
                     FVLNIPPDFSHDLVRGERPAYLVEVDATDPSAVSSAVQALNQIALTAFNRDLEGPLQD
                     LKQGPPPAEMRMHLLYNPDRVTAYNIVPGLMGVVLTMTMVVITALAVTRERERGTMES
                     LLATPALPLEVMTGKIIPYVAVGYVQVTVILIAAKLLFHVPMIGNFALLYLGALCFIV
                     ATLAVGITISTLAQNQLQAMQMSFFFFLPSLLLSGFMFPFRGMPVWAQYLGEALPLTH
                     FLRVLRGILLKGNAFPDIWPDIWPILVFATAVMLLGALRYRRTLD"
     gene            complement(2925..>3351)
                     /locus_tag="VIS74_01655"
     CDS             complement(2925..>3351)
                     /locus_tag="VIS74_01655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5791971.1"
                     /translation="DPKARRDFWEEIHRLAAEGLTVLVTTHYMDEAERCHRLAYIAYG
                     KLLAKGSLREVLHSAGLTTWEVAGPELGKLAARIRDLPGIEQVTAFGSKLHVSGRDRA
                     ALEKAIGPFRREPHGWREIEPGLEDAFIGLMQRSEDNFQ"
BASE COUNT          638 a          990 c         1113 g          610 t
ORIGIN      
        1 ccctttaact ttcccgccat ggtgccgatg tggatgttcc cgctggccct cgcctgcgga
       61 aacgcctttg tgctcaagcc ctcggaacgc gatccgtccg cctccctgct gcttgcggag
      121 ctgctgcggg aggcggggct gcccgatggc gtcttcaacg tggtgcaggg cgacaaggcg
      181 gcggtggacg cattgatcga acatcccggg gtggacgcga tttccttcgt gggttccact
      241 cccattgcgg aatatatcta cacggaatcc agccggcgcg gcaaacgcgt gcaggcgctc
      301 ggcggcgcga agaaccacct cgttgtcatg ccggacgccg acctggatca ggcggcgtcg
      361 gcgttgctcg gcgcggccta cggttccgcc ggcgagcgct gcatggcgat ctccgtggcc
      421 gtcgcggtgg ggccggccgg ggatgcgctc atcgaaaaac tgattccgct ggtaaagggg
      481 ctcaggattg gcaatggcct ggagggcgat ccggacatgg gccccgtggt gaccgccgcg
      541 cataaggccc gcatcgaggg atacctcgag gaaggcgtaa aatccggcgc gaagctgctc
      601 gtggacggcc gcggcctccg ggtggccggt tacgaaaacg gatttttcct cggcggttgt
      661 ttgttcgacg gcgtgacgcc ggaaatgaaa atctaccgtg aggagatctt cggcccggtg
      721 ctcggcgtgg tgcgcgtgcc ggatttggag agcgcccttg agttgatcaa cgcccacgaa
      781 ttcggcaacg gcgtttcctg ctacacgcgc agcggcggag tggcgaggga gttttcccgg
      841 cgcgtgaaga tcggcatggt cggcatcaat gtgcccattc ccgtgccgat ggcctggcat
      901 tccttcggcg gctggaagcg ctcgctcttt ggcggccagc acatctacgg cgaggagggc
      961 gtgcgcttct acacgcgcta caaaagcatc atgcagcgct ggcccgcgga agaccacggc
     1021 gcctcgtttg gaatgccgac gaattagttt ttggaatcga tcaacggatt ccagacccgc
     1081 tcgaatccga acccctgaaa atctttcacg ttcgccgtgg cgaaatgagt gacgccgaag
     1141 gcccggaggg tgagcgccgt tcggctgtca taaatccggc ggcgcgcgaa gttgggcttt
     1201 gccgcgtcgt gccagagcgt atcgtgcaat ttccggctgt gaggtggaaa gccaaaggtc
     1261 tgccagcggg gatgccggcg gtagctttga atgacctccg cggcctcgct ggcggaaagc
     1321 gggcgcacga gcaccgccgg attccggaga tggagataaa actcggcgag cacgaactca
     1381 ctgattccga tatcttcgcg ctggccgatg gattgaagaa aacggcgggc cttctcgtga
     1441 aaaggcgaat ccgtgctgta actgaaaagc agcagattgg cgtcgatcga agtcatcgag
     1501 acgagggcag cgggccttcc tgatcggcca gggcgagatc atgcaattgt tccgccgaga
     1561 gtcccttcca gcccacgcgt tgggaacggg gaggctccca ggcggcggaa accggatgag
     1621 ccggataaac ttcgagcagc atttccgctc cccggcgaaa agtctccgcc agcgaccatt
     1681 cgcgattttt ggcaaaactc cgaagctcct gatagagctc ctccggcaat tggatctgcg
     1741 tttttaccat tccaccaaat taacatatcc attaatttga tgtcaaaatc aatccagcgt
     1801 ccgccggtag cgcagggcgc cgaggagcat gaccgccgtc gcaaagacca ggatcggcca
     1861 gatgtcgggc cagatgtcgg gaaacgcgtt tcccttgagc aggatgccgc ggagcacgcg
     1921 gaggaaatgc gtgagcggca gcgcttcgcc gaggtattgc gcccagacgg gcatgccgcg
     1981 aaagggaaac atgaaaccgg agagcagcag ggaaggcagg aagaaaaaga acgacatctg
     2041 catggcctgg agctgatttt gcgccagcgt ggaaatggtg atgcccaccg cgagggttgc
     2101 gacgatgaaa cacagcgcgc cgagatacag cagcgcgaag tttccgatca tcggcacatg
     2161 aaacagcagc ttcgccgcga tcagaatgac ggtgacctgc acataaccga ccgccacgta
     2221 gggaatgatc ttgccggtca tgacctcgag cggcagcgcg ggggtggcga gcaggctttc
     2281 catggtgccg cgctcgcgct cgcgggtgac ggcgagcgcg gtgatgacca ccatggtcat
     2341 cgtcagcacc acccccatca agccgggcac gatgttgtag gccgtgacgc ggtcgggatt
     2401 gtagagcagg tgcatgcgca tttccgccgg cggcgggcct tgcttcaagt cctggagcgg
     2461 tccttcgaga tcgcggttga aggcggtgag ggcgatctgg ttgagcgcct ggaccgcgga
     2521 gctgaccgcg gagggatcgg tcgcgtccac ctccacgaga taggcggggc gctcgccgcg
     2581 gaccagatcg tgcgaaaaat ccggcgggat gttcaatacg aactgcacct cgccccgcgc
     2641 cagcaattgc cgcgcggcct cctcggtatc gatatgccgg gtgatcttga aatacccgct
     2701 cgtctccatg gcgtgcgcca gccgccgggc gaacgggctg ttgtccgcgg ccagaaccgc
     2761 gacgggcagg cttttgggat cggtgttgat ggcaaaaccg aacagaatga gctgaatgac
     2821 cgggatgccg aacatcatgg cgaatgtcag ccggtcgcgg cgcatctgga tgaattcctt
     2881 gcggatgatg gccgcgaagc gcgcgaggtt gaaggaaagc gggatcattg gaagttgtcc
     2941 tccgaccgct gcatgaggcc gatgaaggcg tcctccagcc ccggttcgat ttcccgccat
     3001 ccatgcggtt cccgccggaa tgggccgatg gccttttcca gcgccgcgcg gtcgcgtccg
     3061 ctgacgtgga gtttgctgcc aaacgcggtg acctgctcga tgccgggcag atcgcgaatc
     3121 cgcgccgcca gttttcccaa ttccggtccg gccacctccc aggtggtcag tccggcggaa
     3181 tgcagcactt cgcgcagcga gcctttcgcc agcaatttcc cgtaggcgat gtaggcgagg
     3241 cggtggcacc gctcggcctc gtccatgtaa tgcgtggtca cgagcaccgt cagcccctcg
     3301 gcggcgagcc ggtggatttc ctcccagaag tcgcgccgcg cctttgggtc c
//
LOCUS       DATDAY010000084         2378 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_1315406, whole genome shotgun sequence.
ACCESSION   DATDAY010000084 DATDAY010000000
VERSION     DATDAY010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2378)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2378)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2378
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1315406"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..556)
                     /locus_tag="VIS74_01660"
     CDS             complement(<1..556)
                     /locus_tag="VIS74_01660"
                     /inference="COORDINATES: protein motif:HMM:NF024842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HEY5791972.1"
                     /translation="MSFTHFLVVGAGFSGAVVARELVEALDCRVTLWEERDHLAGNCH
                     TERDAQTGILLHRHGPHIFNTDRQRVWDYVNRFAPFRPYLNRVKASIPRGIFSFPINL
                     HTINQFFGVRLNPEEARQFLAAKADRSLSQPRNFEEQALSRLGPELYRAFFYGYTRKA
                     WGCEPRELPASFFSRLPVRFNYNDL"
     gene            680..958
                     /locus_tag="VIS74_01665"
     CDS             680..958
                     /locus_tag="VIS74_01665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5791973.1"
                     /translation="MRTTLTIDDDLAGILRRRSKELGKPFKEVVNNALRAGIMEKSTV
                     KPNRVRVRPHDFGLTLANLDYDRLNQLVDELEVEDYLRKAAKDDPAGR"
     gene            939..1376
                     /locus_tag="VIS74_01670"
     CDS             939..1376
                     /locus_tag="VIS74_01670"
                     /inference="COORDINATES: protein motif:HMM:TIGR00028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEY5791974.1"
                     /translation="MILPDVNLLIYAHNVRAPHHSQALDWWNECLAGPNGVALAWVAI
                     LGFVRICTHPKTFERPLAVAEATGRVEEWLSLPHVQLIHPSPNHFQKWSELLRQLGTA
                     GNLTTDAHLAALAIERGLILHTTDADFSRFPGLKWKNPLRPSR"
     gene            complement(1421..>2378)
                     /locus_tag="VIS74_01675"
     CDS             complement(1421..>2378)
                     /locus_tag="VIS74_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEY5791975.1"
                     /translation="IVVAGAGGFIGGHLVAQLRKEGIERIRAVDFKPFGEWHQCFEGV
                     ENLQLDLRRLEACQAAARDAAQVYNFASDMGGMGFIELNKAACMLSVLINTHLLMAAR
                     EAEVRRFFFASSACVYNADKQRDPQVAALKEADAYPAMPEDGYGWEKLFSERMCRHFR
                     EDFGIQTRVARYHNVYGPHGAYEGGREKAPAAICRKVIAAKRSGRLEIEIWGDGNQTR
                     SFQYIDDCIHGTRLLMDSDFAEPINLGSSELVSINQLVDLVEEIAGVRLKRSYRLDAP
                     KGVNGRNSDNTLLRKLFGWEPSTRLRDGLEKTYRWIYDQIAR"
BASE COUNT          529 a          719 c          658 g          472 t
ORIGIN      
        1 aaaggtcgtt gtaattaaag cgcaccggca gccgcgaaaa aaacgaggcc gggagttccc
       61 tcggctcgca gccccaggcc ttgcgggtat agccgtaaaa gaaggcgcga tacagctccg
      121 gccccagccg ggaaagcgcc tgttcctcga aattccgcgg ttgggaaagg gaacggtccg
      181 ctttggcggc cagaaattgc cgggcttcct ccggattcaa ccgcacgccg aagaactgat
      241 tgatggtgtg aaggttgatg ggaaacgaaa aaatcccgcg cgggatgctc gccttcaccc
      301 ggttgagata gggccggaac ggagcgaaac gattgacgta atcccagacc cgctggcggt
      361 cggtgttaaa aatatgcggg ccatgccggt gcagcaggat gccggtttgg gcgtcacgct
      421 ccgtatgaca attccccgcc aggtgatcgc gctcctccca cagcgtcacg cggcaatcca
      481 gcgcctccac caactcgcgg gccaccaccg ctccagaaaa acccgcgccg acgactaaaa
      541 agtgggtgaa ggacatactt caaaagaatg gggataaaac caggcccggc gaacaaattc
      601 cagcttcagc cacgccgaat gaccgcatcc cacaattttg atgccttgca atcaatatta
      661 aatctgccat gatgccttta tgcgaaccac cctgaccatc gacgatgatc tggcgggaat
      721 tcttcgccgt cgttccaagg aattgggaaa accattcaaa gaagtcgtta ataatgcgtt
      781 acgggcggga attatggaaa aatcaaccgt aaaacccaac cgggttcgag tgcgtcccca
      841 tgattttgga ctaacgcttg caaacctgga ctacgaccgt cttaatcaat tggtggacga
      901 attggaagtc gaagactatc tgagaaaggc cgccaaagat gatcctgccg gacgttaacc
      961 tgctcattta cgcccataat gtgcgcgccc cccatcattc acaagccttg gattggtgga
     1021 acgaatgcct ggcgggaccg aacggcgtag ccttggcatg ggtggcgatt ctcgggtttg
     1081 tgcgaatttg cacccatcca aaaaccttcg aacgtccctt ggcggtagcg gaggcaaccg
     1141 ggagggtgga agaatggttg tctttacccc acgtccagct cattcacccc agtccgaacc
     1201 actttcagaa gtggtctgaa ctgttgcgcc aactcggcac cgccggaaat ctcaccaccg
     1261 acgcgcactt ggcggctctg gcgattgaac gcggtttgat tttgcataca acggatgcgg
     1321 atttttcccg tttcccggga ttgaaatgga agaatccgct gagaccctcc cgctaaccac
     1381 aaaggaaacc gccgtttacg gacggttcat cgaaccgtcc ctacctcgcg atctggtcgt
     1441 aaatccagcg ataggttttc tccaggccat cccgcaggcg ggtggagggc tcccagccga
     1501 agagcttccg gaggagcgta ttgtcgctgt tgcggccgtt cacccccttg ggcgcgtcca
     1561 gccggtagct ccgttttaac ctcactccgg caatttcttc gacgaggtcc acgagctgat
     1621 tgatcgaaac cagctcgctg ctgccgagat tgatcggctc ggcgaagtcg ctgtccatca
     1681 acaggcgcgt cccgtggatg cagtcgtcga tgtattgaaa actgcgggtt tggttgccat
     1741 cgccccagat ttcgatttcg aggcggccgc tccgcttcgc cgcgatgacc ttgcggcaga
     1801 tggccgccgg ggctttctcg cggccgccct cgtaggcgcc gtgcgggcca tagacgttgt
     1861 gataccgggc cacccgcgtc tgaattccaa aatcctcacg gaaatgccgg cacatgcgtt
     1921 cgctgaagag tttttcccat ccgtagccat cctccggcat ggcgggataa gcgtccgcct
     1981 ccttcagcgc ggcgacctgc ggatcgcgct gcttgtccgc attgtaaacg caggcggagg
     2041 aggcgaagaa aaaccggcgc acttccgcct cgcgggccgc catcaacaaa tgggtattga
     2101 tcagcacgct caacatgcag gcggctttat tcagctcaat aaaccccatg ccccccatgt
     2161 cggaggcaaa attgtaaacc tgggcggcat cccgcgccgc cgcctggcag gcctcgagcc
     2221 gccgcagatc gagctggaga ttttccactc cctcgaagca ctgatgccat tccccaaatg
     2281 gcttgaaatc cacggcccgg atgcgttcga tcccttcctt gcgaagctgg gccacgagat
     2341 gcccgccgat gaacccgccc gcgccggcga ctacaatc
//
LOCUS       DATDAY010000085         3019 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_2031020, whole genome shotgun sequence.
ACCESSION   DATDAY010000085 DATDAY010000000
VERSION     DATDAY010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 3019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3019)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3019
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2031020"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1538
                     /locus_tag="VIS74_01680"
     CDS             <1..1538
                     /locus_tag="VIS74_01680"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HEY5791976.1"
                     /translation="ILSGCNPKPAPLPKELRKEIARLEREHHSAEEAVKNLPGAAASL
                     TPPPLPEWDQSISPDELRPRIQAALRALLEFRFTLIESRAVAPAQSVSFIAAESGDVF
                     QFTGPPKARTQSLRIENTGSALLVNPRLQANENSDWFDVSSLLDAVLRPGMSDREKVV
                     ALWKFVVASRQHGYRAHPAAELHDPVRFLNVYGYGFCDDASSALTVLASEAGIPTRIW
                     DLSGHVVPEFFFDGKWNLLDPDGEVCYFEKDGVTFADVESLQREPERIREFPSPFYQD
                     AEKLIQIYGTKDDNRLSDFYERTRGQPQRMAYTLRPGEGLWLSRLPAGRYFSADYFQE
                     PPDYGSGRLTFEPVFREGLFQLGAQSVKNLTPVSGGLETTGGESELVYLFSLPYPMLD
                     GRVELECELPEGGSVKVEFSEDGKKWTQAGQSTAPGQQSVSVKLTAFFRNGFGDPVYQ
                     YWLRLRFDSPAGKPARISRLHYQMDVQAAPKSLPQLAGGENTLRFLQSGDGAARVTVD
                     YTE"
     gene            1630..2613
                     /locus_tag="VIS74_01685"
     CDS             1630..2613
                     /locus_tag="VIS74_01685"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016615 - malate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HEY5791977.1"
                     /translation="MKPPLKVAVTGAAGQIGYALLPRIASGSLFGPDQPVELRLLEIP
                     PALKALEGVVMELHDCAFPLLSGVIATDNPDEAFRGANWCLLVGSVPRKAGMERQELL
                     GINGKIFVGQGRAIAQNAAADVRTLVVGNPCNTNCLIAMRSAPEIPRDRWFAMTRLDE
                     NRAKTQLAFKAGTHWTAVSNLAIWGNHSPTMFPDFTHARIHGKPAAEVIADPAWLEGD
                     FLKIVQQRGAAIIAARGLSSAKSAAHAAVETVASIVRPTPAGDWHSVAVCSTGAYGIE
                     PGLITSFPVSSDGQTLSVVEGLELNAFAKSKIDASIHELLDERKMVSGLLA"
     gene            2606..>3019
                     /locus_tag="VIS74_01690"
     CDS             2606..>3019
                     /locus_tag="VIS74_01690"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HEY5791978.1"
                     /translation="MLNVVLVEPEIPPNTGNIARLCLAAGARLHLIEPLGFSIDDRAL
                     RRAGMDYWKDCDIRPWKSWAAFDEAHPDRRAFYLTTKTRRAYWDAEFADGDFLIFGRE
                     TKGLPESLLAANAAHCLTIPMQPNARSLNLATSAGI"
BASE COUNT          532 a         1005 c          917 g          565 t
ORIGIN      
        1 tgatcctttc gggatgcaat cccaaaccgg ctccgctgcc caaggagctt cgcaaggaga
       61 ttgcccggct ggagcgcgag caccattccg cggaggaagc ggtgaaaaac cttccgggcg
      121 ccgccgcgtc tttgaccccg ccgccgttgc cggaatggga ccaatccatt tccccggacg
      181 aactccggcc ccgcattcaa gcggcgctgc gcgccttgct ggaatttcgc ttcacgttga
      241 ttgagtcccg ggcggtggcg cccgcccaaa gcgtgtcgtt catcgccgcg gaatccgggg
      301 atgtctttca gttcaccggc ccgccgaaag cgcgaacgca atccctgcgc atcgaaaaca
      361 ccggctccgc gctgctcgtg aacccgcgcc tccaggcaaa cgaaaacagc gactggttcg
      421 atgtttccag cctgctggat gcagtgctgc ggccggggat gtccgaccgg gaaaaggtgg
      481 tcgcgctttg gaaattcgtc gtcgcctccc gccagcacgg ctaccgggcc catccggcgg
      541 cggaattgca cgatccggtt agatttttaa acgtctatgg ttacggtttt tgcgatgacg
      601 cctcttccgc gttgacggtt ctggccagcg aggccggcat tcccacccgc atctgggatc
      661 tttccggtca cgtcgttccg gaattcttct tcgacggcaa atggaacctc ctcgatcccg
      721 acggggaagt ttgttatttc gaaaaggatg gcgtcacgtt tgccgatgtg gagtcgttgc
      781 agcgggaacc cgaacggatt cgcgaatttc ccagcccgtt ttaccaggac gcggagaaac
      841 tcatccagat ttacgggacg aaggacgaca accggctttc cgacttttat gagcgcacgc
      901 gcggccagcc gcagcgcatg gcttacacgc ttcgtccggg cgaaggactc tggctgagcc
      961 ggctcccggc cggccggtac ttttcggcgg attatttcca ggaacccccg gactacggca
     1021 gcggacgcct gacattcgag ccggttttcc gggagggcct tttccaattg ggcgcccaat
     1081 cggtgaaaaa cctcactccg gtttccggcg gcctggagac caccggcggc gagagcgaat
     1141 tggtttacct gttttcattg ccctatccga tgcttgacgg gcgggtggag ctcgaatgcg
     1201 agctccccga gggcggcagc gtgaaggtcg aattttccga ggacggtaaa aaatggaccc
     1261 aggccgggca atccaccgct cccggccagc aatcggtgtc cgtgaaactg accgcttttt
     1321 tccgcaacgg ctttggggat ccggtttacc aatactggct gcggctgcgg tttgattccc
     1381 ccgcgggcaa gccggcccgg atctcgcgcc tccactacca gatggacgtc caggccgcgc
     1441 ccaagtcgct tccccaactg gccgggggtg aaaacacgct ccgttttctc caatccgggg
     1501 acggcgccgc ccgggtgacc gtggattaca ccgaataaac ccccggcggc tgcggcaatt
     1561 tgcccccccc agcccgttgc gggtttgtgc tttccgaatc gtccgggcgg aagcaggatg
     1621 gaatccgcca tgaaacctcc tctgaaagtc gcagtcactg gcgcggccgg acaaattggt
     1681 tacgcgcttc tcccccgcat cgcttctggt tccctgttcg ggccggatca acccgtcgaa
     1741 ctccgcctgc tggaaatccc gcccgccctc aaggcgctcg agggcgtggt catggagttg
     1801 cacgactgcg cctttcccct gctgagcggg gtcattgcca cggacaatcc cgacgaggcg
     1861 ttccgcggcg cgaactggtg cctgctcgtg ggcagcgttc cgcgcaaggc cggcatggaa
     1921 cggcaggagc tcctcggcat caatggcaaa atcttcgtcg gccagggccg ggccatcgcc
     1981 caaaacgcgg ccgccgatgt gcgcacgctc gtcgtcggca atccgtgcaa tacgaattgc
     2041 ctcattgcca tgcgcagcgc gccggagatt ccacgcgacc gctggttcgc catgacgcgg
     2101 ctggacgaaa accgggccaa gacgcagctc gcattcaagg cgggaacgca ttggaccgcg
     2161 gtcagcaatc tcgcgatctg gggcaatcac tcgccgacca tgttccccga cttcacccac
     2221 gcccggattc acggaaagcc cgccgcggaa gtcatcgccg atcccgcctg gctggagggc
     2281 gactttctca aaatcgtgca gcagcgcggc gccgccatca tcgcggcccg cggcctctcc
     2341 tcggccaagt cggctgccca tgccgccgtg gagacggtgg cctcgatcgt gcgcccgacc
     2401 ccggccggcg actggcacag cgtcgccgtt tgttcgacgg gcgcctacgg catcgagccg
     2461 gggctgatca cgtcctttcc cgtgagttcg gacggccaaa ccctctccgt cgtcgaaggg
     2521 ctggagctga acgccttcgc gaaatcgaaa atcgacgcgt ccattcacga gctgctggat
     2581 gaacgcaaaa tggtctccgg cttgcttgct taacgtcgtc ctggttgaac ccgaaatccc
     2641 gccgaacacg ggaaacatcg cccggctgtg cctggccgcc ggcgcgcgcc tgcacctgat
     2701 cgagccgctg ggcttctcga ttgacgaccg ggcgctgcgc cgcgccggca tggactactg
     2761 gaaggattgc gacatccgcc cctggaaatc ctgggcggcg tttgacgagg cccacccgga
     2821 ccggcgcgcc ttttatttga ccacgaaaac ccggcgggcg tattgggacg cggaattcgc
     2881 cgacggcgat tttctcatct tcggccggga aaccaagggc ctgccggaat ccctgctggc
     2941 ggcgaatgcg gcgcattgcc tgaccattcc catgcagccg aacgcccgca gcctgaacct
     3001 ggccacctcg gcgggcatc
//
LOCUS       DATDAY010000086         2319 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_2173276, whole genome shotgun sequence.
ACCESSION   DATDAY010000086 DATDAY010000000
VERSION     DATDAY010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2319)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2319)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2319
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2173276"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            27..617
                     /locus_tag="VIS74_01695"
     CDS             27..617
                     /locus_tag="VIS74_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEY5791979.1"
                     /translation="MRKIRIFEHISLDGVIQPLGGPNEDSDYAHGGWKAPYRSAAGAE
                     AIAEAQGKGFDLLLGRRTYDLWADFWPTVKGGPFADSLNAATKYVATHRPGSLGWGPV
                     GDLGADVMESIRRVKSEVGPDLIIWGSATLTCVLLEQGLVDEVVLIVYPILLGRGKRP
                     FPDSADPRELALVSSKATTSGVLFNTYRHVGSLRTA"
     gene            746..1153
                     /locus_tag="VIS74_01700"
     CDS             746..1153
                     /locus_tag="VIS74_01700"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY5791980.1"
                     /translation="MSTKIFINLPVKDLPKSMEFFRALGFTFNPQFTDETAACMVISE
                     DIYSMLLTEEKFKDFTPKPICDARKCTEVLVALTRESREAVDALVAKAVAAGGGTYKE
                     PPDHGFMYAYGFQDLDGHIWEPFWMDPSAIQQN"
     gene            1174..1710
                     /locus_tag="VIS74_01705"
     CDS             1174..1710
                     /locus_tag="VIS74_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEY5791981.1"
                     /translation="MIIKILLGLAILVAALAVFIATRPSDFRITRSAVLPAPPSVVFP
                     QVNDLHKWEAWSPWAKLDPNSKTTFDGPPAGTGSSMAWAGNEQVGEGKMTITESKLNE
                     LILMRLEFIKPFQATNATEFTFKPEGSGTLVTWTMSGQNNFVGKAIGLFMNCEKMLGG
                     QFEQGLDNLKKVVSTGQN"
     gene            1730..2236
                     /locus_tag="VIS74_01710"
     CDS             1730..2236
                     /locus_tag="VIS74_01710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY5791982.1"
                     /translation="MKTLAQRITPFLWFDRQAEEAVNDYISIFKNSKITSVTRYDEAG
                     AKASGQPAGTVMTVAFQLDGQDFVAINGGPHFQFSCAVSFVVNCETQDEVDYYWEKLS
                     AGGDETAQQCGWLADKYGLSWQVVPTVVPELLTAGDPEKSRRVMQAVLQMKKIDIETV
                     LKAAEDRA"
BASE COUNT          507 a          734 c          645 g          433 t
ORIGIN      
        1 aaacatctaa ctgaagcagg cacaaaatga gaaagatcag gatcttcgaa catatctcgc
       61 tggacggcgt gatccagccg ctcggcggac cgaacgaaga cagtgactac gcgcatggcg
      121 ggtggaaggc accgtatcga agtgcggcgg gggcagaggc catcgccgag gcacagggca
      181 aaggtttcga tctgctgctt ggccgccgca cctacgatct ttgggccgat ttctggccga
      241 cggtcaaggg cggtccgttc gcggacagtc tgaacgccgc gacgaaatac gtcgcaaccc
      301 acaggccggg cagccttggt tggggtccgg tcggagacct aggcgcggac gtcatggaga
      361 gtattcgccg cgtcaagtca gaggtcggcc ctgacctgat catctgggga agtgcgacgc
      421 tgacgtgcgt gctgctcgag cagggattgg tcgacgaggt tgtgctgatc gtctatccga
      481 tcttgctggg ccggggcaaa cgcccatttc cagacagtgc ggaccctcgt gaacttgctc
      541 tcgtcagctc aaaggccacg acttcaggcg tgctttttaa cacttatcga catgtcggct
      601 cgctgcgaac cgcatgaact tagggcggcc atgcactgga gcgaaccgct ctgggcgtcc
      661 cttccgtttg cttgtcaaca gcttcgcggt cgctcaattt ggttcgatca actgaatatc
      721 caaccaaccc ccccattttc aactcatgag caccaaaatc ttcatcaacc tcccggtcaa
      781 agacctgccc aagtccatgg aattcttccg cgccttgggc ttcaccttca acccgcaatt
      841 caccgacgag acggccgcct gcatggtcat cagcgaagat atctacagca tgctgctgac
      901 cgaggaaaaa ttcaaagact tcacgccgaa accgatttgc gatgcccgca aatgcaccga
      961 agtgctcgtc gccctcaccc gcgagagccg cgaggcggtg gatgcgcttg tggccaaagc
     1021 cgtcgccgcc gggggcggca cctataagga gccgccggac cacggcttca tgtatgccta
     1081 cggcttccag gatctggacg gccacatctg ggagcccttc tggatggatc cgagcgccat
     1141 tcaacaaaac tgaacaccca tatcccaacc accatgatca tcaaaatcct cctcggcctc
     1201 gccatcctag tcgcggcgct tgccgttttc atcgccaccc gcccctccga cttccggatc
     1261 acccgctccg cggtccttcc cgcgccgcct tccgtggtct tcccgcaggt gaacgacctt
     1321 cacaaatggg aggcctggtc accctgggcg aagctggatc cgaattccaa aaccactttc
     1381 gacggcccgc ccgccggcac cggttcctcc atggcctggg ccggaaatga acaggtcggc
     1441 gaaggaaaaa tgaccatcac cgaaagcaag ctcaacgagc tgatcttgat gcggctcgaa
     1501 ttcatcaaac ccttccaggc gaccaatgcg accgaattca cgttcaagcc cgagggcagc
     1561 ggaaccctcg tgacctggac catgtcgggt caaaacaact tcgtgggcaa ggccatcggc
     1621 ctcttcatga actgcgaaaa gatgctgggc gggcagttcg agcagggctt ggacaacctg
     1681 aagaaggtgg tttccaccgg ccagaattaa ccgacgccta aaaatcctta tgaaaaccct
     1741 cgcgcaaaga atcactccct ttctctggtt tgaccgccag gccgaggagg cggtgaacga
     1801 ttacatttcg atcttcaaga actcgaaaat cacgtcggtc acccgatacg acgaggccgg
     1861 ggccaaagcc tccgggcagc cggcgggaac ggtcatgacc gtggccttcc aactcgacgg
     1921 gcaggacttt gtggccatca acggcgggcc tcatttccag ttcagctgcg ccgtgtcctt
     1981 cgtcgtcaac tgcgagaccc aggacgaggt ggattattat tgggaaaaac tttccgcggg
     2041 cggggatgag accgcgcaac aatgcggctg gctggcggac aagtatggcc tctcctggca
     2101 ggtcgttccc acggtggtgc ccgaattgct gacggccggg gatcccgaaa agtcccggcg
     2161 cgtcatgcag gccgtcctgc aaatgaagaa gatcgacatc gaaacggtcc tgaaagcggc
     2221 ggaggaccgc gcctgatccc accatcaacc cgcacccctt atgcccactc ccgccaatcg
     2281 cgaactcgcc atcacccggg atgttcccgt tcccgccgc
//
LOCUS       DATDAY010000087         2677 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_203648, whole genome shotgun sequence.
ACCESSION   DATDAY010000087 DATDAY010000000
VERSION     DATDAY010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2677)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2677)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2677
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_203648"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1851)
                     /locus_tag="VIS74_01715"
     CDS             complement(<1..1851)
                     /locus_tag="VIS74_01715"
                     /inference="COORDINATES: protein motif:HMM:NF015089.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase domain-containing protein"
                     /protein_id="HEY5791983.1"
                     /translation="MTNRPDLANDPVLFGSGKRTRIVAVEFLEPDRIQLYQRGEDGST
                     QTAEEPFQPYLWAREGGSALGGRLPLNHRQTFATWAEFQTARKADPSSFSSNDPVQQY
                     LVATGETHFKEMAFEDLRRLQLDIETACEEGFAFSNAARDPILAIALSDSTGWEECLI
                     AEDAAGEKAALERLTELIRERDPDVLEGHNLFKFDLPYLAARAKKLKVKLAWGRDGSP
                     LGLRSSRLQIAEKAIQYSKPSIHGRHVVDTWVLAQLYDVSSRELESFGLKAVARHFGI
                     AESERVYLPGTEIQNAFRGDRENFIRYAIHDVRETRALAALLSRSYFIQAQIFPFSYQ
                     DVIIRGNATRIDSLFLREYLRQRHSIPEFPNAEGFEGGYTDIFKTGVLSGVWHCDVAS
                     LYPSVMLQFDLLPANDELKIFRQLLANLRAFRLAAKQRLRGAANETEANYFNALQTAF
                     KILINSFYGYLGFAQGHFADFQTAAAVTAKGREILQHLVGWRGRRGAAVIEIDTDGIY
                     FQPPAGATPDGLQRELQAELPAGIEVELDQQYAAMFSYKTKNYALLETGGRMVIKGAA
                     LKSRGLEKFLRDYLERLLRFLLEGRSAEAAGLRDSFAQKIRAREWPIEMLA"
     gene            1896..>2677
                     /locus_tag="VIS74_01720"
     CDS             1896..>2677
                     /locus_tag="VIS74_01720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5791984.1"
                     /translation="MSDPEFISPSESLDQLAPGVVLGGRYSLKSRVPRWIADRRFKAR
                     DLESGETVAVLVWPEETPWFGELVRRLEALPEPIHRYAGSLQGYGVVILNWRNGLRAP
                     KGSADSAEEVLRLLRNSVPARRKRAPLPPRREFEPEPASPANPVVLRNPWTEWIGFGL
                     AVVVALAVIGFTFQLGQALRKPVVIDPGLAGLAPKSAPAPVSLDGRLKEAETLRKNGN
                     ATAALGLLLELDANQPGNARITQPINSLLDSLDAELPAMKLGL"
BASE COUNT          453 a          826 c          905 g          493 t
ORIGIN      
        1 ggcgagcatt tcgatgggcc attcgcgcgc gcggattttt tgggcgaagg aatcgcgcag
       61 ccccgcggct tcggcggacc tgccttcgag caggaaacgc aggagccgct caagatagtc
      121 gcgcaaaaat ttttccagcc cgcgggattt gagcgcggcg cccttgatga ccatgcgccc
      181 gccggtttcc agcagcgcgt agtttttggt tttgtagctg aacatcgccg cgtattgctg
      241 atccagctcg acttcgatgc ccgccggcag ctcggcctgg agttcgcgct ggaggccgtc
      301 cggcgtggct ccggcgggcg gctggaaata aatgccgtcc gtgtcgattt cgatgaccgc
      361 cgcgccgcgc cgcccgcgcc agccgaccag gtgctgcagg atttcccggc ccttcgcggt
      421 gaccgccgcg gcggtctgga agtcggcgaa atggccctgc gcgaagccga gataaccgta
      481 gaagctgttg atcaaaattt taaacgccgt ctgcagcgcg ttaaaataat tcgcctccgt
      541 ctcgttggcc gccccgcgca gccgctgctt ggcggcgagc cggaaggcgc ggaggttcgc
      601 gagcaattgg cggaagatct tcaactcgtc gttcgcgggc agcaggtcga attgcagcat
      661 caccgaggga tacagcgacg ccacgtcgca gtgccacacg ccgctcagca cgccggtctt
      721 gaagatgtcg gtgtagccgc cctcgaagcc ttcggcattt ggaaattcgg gaatcgagtg
      781 ccgctggcgc aggtattcgc gcaggaagag cgaatcgatc cgcgtcgcgt tgccgcggat
      841 gatgacgtcc tgatagctga acggaaaaat ctgcgcctgg atgaaataac tccggctgag
      901 cagcgccgcg agcgcgcgcg tttcgcgcac gtcgtggatg gcgtaacgaa tgaagttctc
      961 gcggtcgccg cgaaaggcgt tctgaatttc cgtgccgggc agatacacgc gctcgctttc
     1021 ggcgatgccg aaatgccgcg cgaccgcctt gaggccgaag ctttcgagtt cgcggctgga
     1081 cacgtcgtaa agctgcgcga gcacccacgt gtccaccacg tgccgcccgt gaatggacgg
     1141 cttggaatat tggatcgcct tctccgcgat ttgcaggcgc gaggaacgga ggccgagcgg
     1201 ggagccgtcg cgcccccagg cgagcttgac cttgagcttt ttggcgcgcg cggcgaggta
     1261 gggcaaatcg aacttgaaga ggttgtgccc ttcgaggacg tccggatcgc gctcgcggat
     1321 cagctcggtc agccgctcca gcgccgcctt ctcgcccgcg gcgtcttcgg cgatgaggca
     1381 ttcctcccag ccggtcgaat cgctcagcgc gatggccaga atcggatcgc gcgccgcatt
     1441 ggaaaacgcg aaaccctcct cgcaggcggt ttcgatgtcg agctgcaggc ggcgcagatc
     1501 ctcgaaggcc atttccttga aatgggtttc gccggtggcg acgaggtatt gctggacggg
     1561 atcgttggag gaaaacgagg agggatcggc cttgcgcgcg gtttggaatt cggcccaggt
     1621 ggcgaaggtt tgccggtggt tcaacggcag ccgcccgccc agcgccgagc cgccttcccg
     1681 ggcccacagg tagggctgaa acggctcctc cgcggtttgg gtggagccgt cctccccgcg
     1741 ctggtaaagc tggatgcggt ccggctccag aaactccacg gcgacgatgc gcgtccgttt
     1801 tcccgaacca aaaagcaccg ggtcattcgc gaggtcgggg cggtttgtca ttgtttggga
     1861 ttaagcggag ctctatcaca ttgtgcaacc ctaacgtgag cgaccccgag ttcatttccc
     1921 catccgagag cctggaccag ttggcccctg gggtggtttt gggcgggcgt tattccttga
     1981 agagccgcgt gccgcgctgg atcgcggatc gccgcttcaa ggcgcgggat ctggaatccg
     2041 gcgaaacggt ggccgtgctg gtctggccgg aggaaacgcc gtggtttggc gagctggtcc
     2101 gccggctcga ggccctgccg gaaccgattc atcgctacgc cggttcgctc cagggttacg
     2161 gcgtggtgat cttaaattgg cgcaatggcc tgcgcgcccc caagggaagc gcggattcgg
     2221 cggaggaggt gctgcggttg ctccgcaaca gcgtgccggc ccggcgaaag cgcgccccct
     2281 tgccgccccg gcgcgaattt gagccggagc ccgcctcgcc ggcgaacccc gtggttttac
     2341 ggaatccctg gacggagtgg atcggcttcg gccttgcggt ggtggtcgcg ctggcggtca
     2401 tcggattcac cttccaactc ggccaggcgc tgcgcaaacc ggtggtgatc gaccccggcc
     2461 ttgccgggct cgcgccaaaa tccgcccccg cgccggtgtc gctcgacggc cggctgaagg
     2521 aggcggaaac cctgcgcaaa aacggcaatg ccaccgcggc gctcgggttg ctgctcgaac
     2581 tggacgcgaa ccagccggga aatgcgcgga ttacccagcc gatcaacagc ttgctggatt
     2641 cgctggacgc cgagctgccg gcgatgaaac tggggct
//
LOCUS       DATDAY010000088         2331 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_940586, whole genome shotgun sequence.
ACCESSION   DATDAY010000088 DATDAY010000000
VERSION     DATDAY010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2331)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2331)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2331
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_940586"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..704)
                     /locus_tag="VIS74_01725"
     CDS             complement(<1..704)
                     /locus_tag="VIS74_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006983234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthetase B"
                     /protein_id="HEY5791985.1"
                     /translation="MCGIAGFAGSGARADLARMTRALAHRGPDAEGLWQDEARGVYLG
                     HRRLSIIDLGGGGQPMWSADGKIGIVFNGEIYNHAELRRELQQAGRQFLTGHSDTEVL
                     IHGYREWGDDFVNRLNGMWAFVIYDAERRRLFASRDRFGKKPFYYFQSGETFAFGSEL
                     TAMTAHPAAPSGLDRLAIAKYFAYGLIPAPLTMVRGIRKLPAGHNLEFSLASRELRVS
                     KYWEYRIEPGEAWGSP"
     gene            complement(817..1758)
                     /locus_tag="VIS74_01730"
     CDS             complement(817..1758)
                     /locus_tag="VIS74_01730"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006983235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEY5791986.1"
                     /translation="MISITVPIYNERESVLPLHDKIREVMLKLDRPWELIFVNDGSHD
                     GTAELLDGLAARFPEVKVIHFRRNFGQTAAMMAGFDFARGEVIIPLDGDYQNDPEDIP
                     RMLAKLNEGFDVCSGWRRDRQDHALRRNLPSIFANKLISYVSGVRLHDFGCSLKAYRS
                     EVIKGVRIYGEMHRFLPIYAKWHGARITEIPVNHFARRSGSSKYGLERVVKVLFDLMT
                     VKFLDKYALKPMYLFGLVGFLSLFAGFAGFMWALGLYFFASTPLITTPLPLMTTFATL
                     TGIMCLLMGLLAEMITRTFYESQGKTVYLIRETKNLS"
     gene            1904..>2331
                     /locus_tag="VIS74_01735"
     CDS             1904..>2331
                     /locus_tag="VIS74_01735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5791987.1"
                     /translation="MKISPYFFGLACLAAAWMSGFSGCATAPLAQPDAAHRISEKGIG
                     PIRLGMTSAQVQAALGPGYRIASPARGNTLQLAAWHRVLNAGNQEVFRFFVSDWRNPG
                     RPSPIIRVRTTQGDYRTAEGIGPGAPIPTAVNEWGAAQLRR"
BASE COUNT          452 a          709 c          727 g          443 t
ORIGIN      
        1 tgcggcgaac cccacgcctc gccgggttcg atccggtatt cccaatattt cgagacgcgc
       61 agctcgcggg aggcgaggga aaattccagg ttgtgcccgg cgggcagctt gcgaatgcct
      121 cgcaccatgg tcaacggcgc gggaatcagc ccgtaggcga aatatttggc aatcgccagc
      181 cggtccagcc ccgagggcgc ggcgggatgc gccgtcatcg ccgtcaattc cgatccaaac
      241 gcgaaggttt cgcccgattg aaaataataa aacggcttct tgccgaagcg gtcccggctg
      301 gcgaagaggc gccggcgttc cgcgtcgtaa atgacgaacg cccacatgcc gttgaggcgg
      361 ttgacgaaat catcgcccca ttcgcggtag ccgtgaatga gcacctccgt gtcggaatgg
      421 ccggtcagaa attggcgccc cgcctgctga agctcccggc gcagttcggc gtgattgtag
      481 atttcgccgt tgaaaacgat gccgattttc ccatccgcac tccacatcgg ctgcccgccg
      541 ccacccaggt cgatgatgct caggcgccgg tggccgagat aaacgccgcg ggcttcgtcc
      601 tgccagagcc cctccgcgtc cggtccgcgg tgggccagcg cccgggtcat ccgcgccagg
      661 tccgctcgcg cgcccgaacc ggcgaatcca gctattccgc acatgactgg tgaatggtga
      721 ctggaagagg ggggaggggg atcggaagtc cgaaatccga aatccgaaat ccgaaatccg
      781 aaatcatctc tcccccggcc ctttcgctct tccctttcac gagaggtttt tcgtctcgcg
      841 aatcaggtaa accgttttcc cctgggattc gtaaaaggtg cgcgtgatca tttccgcgag
      901 caggcccata agcaggcaca tgattcccgt cagggtggcg aaggtcgtca tgagcggaag
      961 cggggtggtg atgagcggcg tggacgcgaa aaaataaagc cccagcgccc acataaaacc
     1021 ggcgaaaccg gcgaataaac tcaggaaccc gaccaacccg aagagataca tcggcttcaa
     1081 cgcgtatttg tcgaggaact tgaccgtcat gaggtcgaac agcaccttga cgacgcgctc
     1141 caatccgtat ttggacgaac cgctgcggcg ggcgaaatgg ttgaccggga tttcggtgat
     1201 gcgcgcgccg tgccatttgg cgtagatcgg caggaagcgg tgcatctcgc cgtaaatgcg
     1261 cacgcccttg atgacttcgc tccggtaagc cttcagggag cacccgaagt catgcagccg
     1321 caccccggag acatacgaga tgagcttgtt ggcgaagatg ctggggaggt tccggcgaag
     1381 ggcgtgatcc tgccggtcgc gccgccagcc ggagcagacg tcgaatccct cgttgagctt
     1441 tgcgagcatg cgcggaatgt cctcgggatc gttctggtag tcgccgtcga ggggaatgat
     1501 cacctcgccg cgggcgaagt cgaatcccgc catcatggcc gccgtctgcc cgaaattccg
     1561 ccggaaatgg atgaccttga cctcgggaaa tcgcgccgcc agcccgtcca acagttccgc
     1621 ggtgccatcg tggctgccat cgttcacgaa gatcagctcc cacggccggt ccagtttgag
     1681 catgacctcg cggatcttgt catgcagggg caggacggat tccctttcgt tgtaaatggg
     1741 gacggtaatc gaaatcatcg aacattccat tagagcaggt tgggcgattg gctccagact
     1801 gaaaacgggg attttatcgt ttgcgcccga gccggacggc ttgttattac ttcgcatttg
     1861 atgggcgagc ctttggtttc cgggccgctt ttggataaaa gagatgaaaa tttcccccta
     1921 tttttttgga ttggcgtgcc tggcggccgc ttggatgtcg gggttttccg gctgcgcgac
     1981 cgcgccgctg gcccagccgg acgccgccca tcggatctcc gaaaagggca tcggtccgat
     2041 ccggctcggc atgacttccg cccaggtgca ggcggcgctt gggccgggat accgcatcgc
     2101 cagcccggcg cggggaaata cgctgcaact cgccgcctgg catcgtgtgc tcaatgccgg
     2161 caatcaggaa gtctttcgtt tctttgtgag cgattggcgg aatcccggcc ggcccagccc
     2221 gatcatccgc gtgcgcacca cgcaggggga ttaccgcacg gccgaaggca tcggcccggg
     2281 cgcgcccatt cccacggccg tcaatgaatg gggcgccgcg cagctgcggc g
//
LOCUS       DATDAY010000089         3634 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_940303, whole genome shotgun sequence.
ACCESSION   DATDAY010000089 DATDAY010000000
VERSION     DATDAY010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 3634)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3634)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3634
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_940303"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..679
                     /locus_tag="VIS74_01740"
     CDS             <1..679
                     /locus_tag="VIS74_01740"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY5791988.1"
                     /translation="SQADFERHVNAAPTKEFIARLKGGVEGDGPKVTLLRPSPGAVSP
                     GAARSGQADASLPRGIDHVGLTVPDVDEAARFFEKAFGATAIYDVQPEGAKPMAGPDV
                     ERELGLPPGAKIVHMRLMRLGKGPSLELFRIEDAAHSPAAALNDYGWTHIALYVDDIN
                     AAAKRFEDAGGTLLSPPHGLAGVESGEKNLGLYGKAPWGGLIELLTYPSGIRYPDPQL
                     TRWTPAR"
     gene            704..1201
                     /locus_tag="VIS74_01745"
     CDS             704..1201
                     /locus_tag="VIS74_01745"
                     /inference="COORDINATES: protein motif:HMM:NF018777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EthD domain-containing protein"
                     /protein_id="HEY5791989.1"
                     /translation="MSQKNALPTRRRFLSTALLGAAAAAAAPLLARAQSDPNRQPERK
                     PDMIKMTMFLKRNPKLTHEQFVDHHLNHHGPLFRSIPEAKIHVIRYLQTHPIAEKSKV
                     IAVNDFDGTAELWFDSVAGLDAVLTSETYKTTVFPDELTFLDHKNTLVQLGTQADIMG
                     GAGSA"
     gene            1231..2118
                     /locus_tag="VIS74_01750"
     CDS             1231..2118
                     /locus_tag="VIS74_01750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY5791990.1"
                     /translation="MNTIETPHPAAQSGPFPIGGDRPVHRLGFGAMRITGKGVWGEPA
                     DRKECIAVLRRAVDLGITLIDTADSYGPFVSEKLIAEALHPYPENLVIATKAGLQRPG
                     PDEWVPDGRPAHLRERCEGSLVRLKVDRIDLFQLHRIDPKVPLEDQIGTLLDLQREGK
                     IRHLGLSEVNVAEIKAARRLAPIATVQNLYNLADRHSEAVLDYCTRENIGFIPWFPLA
                     TGSLADSDGPLAHAAARLGATPGQVALAWLLKKSPVMLPIPGTSKVEHLESNTHAALL
                     EIDGHLEKELEASAATATH"
     gene            2221..>3634
                     /locus_tag="VIS74_01755"
     CDS             2221..>3634
                     /locus_tag="VIS74_01755"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoamylase"
                     /protein_id="HEY5791991.1"
                     /translation="MNLLSRIGQSAPLGATILDGGVNFSLFSRYATGVELLLFDREED
                     ARPERVITIDPASDRTYHYWHTFVPDVEPGQLYGYRVSGPFDPARGLRFDPGKVLLDP
                     YGRGVVVPGSYDREAARREGDNTATAMKSVVVDPRAYDWEGDKPLNRSWSRTIIYEMH
                     VRGFTRHPSANVSEDKRGTFAGLIEKIPYLRELGITAVELLPVFQFDAQDCPPGLVNY
                     WGYAPVSFFAPHQAYSSRQDRLGPVDEFRDMVKALHRAGIEVILDVVFNHTAEGGHTG
                     PTQCFRGLDNGAYYLLEPDRSRYANYSGTGNTLNANHPIVRRMILDSLRHWAGEMHVD
                     GFRFDLASILARGDAGQVMPNPPVLWDIESDPALAGTKLIAEAWDAGGLYQVGSFVGD
                     SWKEWNGRFRDDVRGFLRGDECSVRAFADRMLGSPEIYGYEEREAEQSVNFVTCHDGF
                     TLNDLVSYNEKHNEANGEGNR"
BASE COUNT          693 a         1208 c         1114 g          619 t
ORIGIN      
        1 atcccaggcc gattttgaaa ggcacgtgaa cgccgcgccc acgaaggaat ttatcgccag
       61 gctcaaaggg ggcgtggaag gcgatgggcc gaaagtgacc ctgcttcgcc cctcgccggg
      121 agcggtgtcg ccgggcgcgg cgcgttcggg ccaggccgat gcttcgcttc cccggggcat
      181 cgatcatgtc gggctcaccg ttcctgatgt cgatgaagcc gcgcgtttct tcgaaaaagc
      241 cttcggcgcc acggccatct atgacgtcca gcccgaaggc gccaagccga tggccgggcc
      301 ggacgtcgaa cgggagctgg gattgccgcc cggcgcaaag attgttcaca tgcgcctcat
      361 gcgtctaggc aagggtccgt cgctcgaact tttccgaatc gaagacgcgg ctcactcgcc
      421 cgcggccgcc ttgaacgatt acggctggac ccatatcgcc ctttacgtgg acgacatcaa
      481 cgccgccgcc aagcgtttcg aagacgcggg cggaaccctg ctctccccgc cgcacggcct
      541 ggcgggcgtc gagtccgggg aaaagaacct cggcctttat gggaaagcgc cctggggcgg
      601 gctgatcgag ctgctcacct acccttccgg catccgatac cccgatccgc aactcacccg
      661 ctggaccccg gcacgctgat taccgcttat gaaaggaaaa cccatgagcc agaaaaatgc
      721 cctgccgacg cggcgccgct tcctctccac cgccctgctc ggcgcggcgg ccgcggcggc
      781 ggcgccattg cttgcccgcg cgcagtccga tcccaaccgc caacccgaaa ggaaacctga
      841 tatgatcaag atgacgatgt ttttaaaacg gaatcccaag ctcacgcatg agcaatttgt
      901 ggatcaccac ctcaaccacc atggcccgct tttccggtcg atcccggaag ccaaaatcca
      961 cgtgatccgc tacctgcaga cgcatcccat tgcggaaaaa tccaaggtca tcgcggtcaa
     1021 tgatttcgac ggaaccgccg agctctggtt cgacagcgtg gccgggctcg atgccgtcct
     1081 cacctcggag acctacaaaa ccacggtgtt cccggacgaa ctgacgttcc tcgaccacaa
     1141 aaacaccctc gtccagctcg gaacccaggc ggacattatg ggtggcgccg gttcggcgta
     1201 ggcaactcca aaccacgaat tcccacgcaa atgaacacca ttgaaacccc acatcccgcc
     1261 gcccaaagcg gcccattccc catcggcggc gaccggccgg ttcatcggct cggcttcggc
     1321 gccatgcgga tcaccggcaa aggcgtctgg ggagagccgg cggaccgcaa ggaatgcatc
     1381 gccgtgctgc ggcgcgccgt ggatcttggc atcacgctca ttgataccgc ggattcctac
     1441 ggcccgtttg tttccgagaa actgatcgcc gaggcgctgc atccgtatcc ggaaaatctg
     1501 gtcatcgcca ccaaggccgg gctccagcgt ccggggcccg atgaatgggt tccggatggg
     1561 cggcccgccc atttgcgcga acgctgcgaa ggcagcctcg ttcgtttgaa agtggaccgg
     1621 atcgatctgt tccaacttca ccggatcgac cccaaggtgc cgcttgagga tcagatcgga
     1681 accctgctcg accttcagcg cgaagggaaa atccgccacc tcggcctgtc cgaggtgaat
     1741 gtggccgaaa tcaaggcggc ccgccgcctg gcgcccattg cgacggtgca gaatctctac
     1801 aacctcgccg accgccattc cgaggcggtg ctggattact gcacgcggga aaatatcggg
     1861 ttcattccgt ggttcccgct ggcgaccggc agcctcgccg attcggacgg cccgctcgcc
     1921 cacgccgccg cgcggctcgg ggcaactccc ggccaggtgg ccctggcgtg gctgctcaag
     1981 aaatcgccgg tcatgctgcc gatcccgggc acctcgaaag tagagcattt ggaaagcaac
     2041 acccacgccg cgcttctcga gatcgatggc catcttgaga aggaattgga ggcaagcgcc
     2101 gccacggcga cccattagcc ggccggtccc gcattggtgc ggcagcggcg aaataggttt
     2161 cccaatggcg gggaaacgtg gcagattgtt ttcggtccgg ccgaagcgtg gccactcccc
     2221 atgaacttgc tgtcacgaat cgggcaaagc gctcccctcg gcgccacgat tttggacggc
     2281 ggcgtgaatt tcagcctctt ttcgagatac gccacaggcg tggagttgct tctctttgac
     2341 cgcgaagaag acgcccgccc ggagcgggtc attacgatcg atcccgcctc ggatcgcacg
     2401 tatcactact ggcacacgtt cgtgccggac gtggagccgg gacagctcta tggctatcgg
     2461 gtcagcgggc cattcgatcc cgcgagaggg ctgcgttttg accccggcaa ggtgctgctc
     2521 gatccctacg gccgcggcgt ggtggtgccc gggagttacg accgcgaggc cgcccgccgc
     2581 gaaggcgaca acacggcgac ggcgatgaaa agcgtggtcg tggacccgcg cgcctacgat
     2641 tgggagggag acaagccgtt gaaccgttcg tggtcgcgga cgatcatcta cgagatgcac
     2701 gtgcggggat tcacgcgcca ccccagcgcg aacgtttcgg aagacaagcg cggcaccttc
     2761 gccgggctca ttgaaaaaat tccgtatctc cgggaactcg gcatcaccgc cgtcgagttg
     2821 ctgccggtgt tccaattcga cgcgcaggac tgcccgcccg gactcgtcaa ctactggggt
     2881 tacgcgccgg tctccttctt tgcgccccac caggcctaca gctcgcggca ggaccggctc
     2941 ggtccggtgg acgagttccg cgacatggtg aaggcgctgc accgggcggg catcgaggtc
     3001 attctcgacg tggtgttcaa tcacaccgcc gagggcggcc acactgggcc gacccagtgc
     3061 ttccgcggac tggataacgg cgcctattac ctcctcgagc ccgaccgctc gcgctacgcg
     3121 aactacagcg gcaccggaaa cacgctgaac gccaaccacc ccatcgtccg ccgcatgatc
     3181 ctcgacagcc tccgccattg ggccggggag atgcacgtgg acggcttccg ttttgatctc
     3241 gcgtcgattc tcgcgcgcgg cgacgccggc caggtgatgc cgaaccctcc cgtgctctgg
     3301 gacatcgagt ccgacccggc gctcgcgggc acgaaactca tcgccgaagc ctgggacgcc
     3361 ggggggctct atcaggtggg cagcttcgtc ggggacagtt ggaaggaatg gaacggccgc
     3421 ttccgcgacg atgtgcgcgg ttttctccgc ggggacgaat gttcggtgcg ggcgttcgcc
     3481 gaccggatgc tgggcagtcc ggagatttac ggctacgagg agcgcgaggc cgaacagagc
     3541 gtgaacttcg tgacctgcca cgacgggttc accctcaacg acctcgtctc ctataacgag
     3601 aaacacaacg aagccaacgg ggagggcaat cgcg
//
LOCUS       DATDAY010000090         3458 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_2075533, whole genome shotgun sequence.
ACCESSION   DATDAY010000090 DATDAY010000000
VERSION     DATDAY010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 3458)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3458)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3458
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2075533"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            98..1177
                     /locus_tag="VIS74_01760"
     CDS             98..1177
                     /locus_tag="VIS74_01760"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY5791992.1"
                     /translation="MTLIGNDDGKTQNPPSKTVLVAGAHGVTGRYAALHFARQTEVAV
                     YGLSRRLDELPGVHFLSADLLNLPDVEAKLAGIPAITHLVFAAYVERPTAKERSEVNT
                     ALLRNLLDGLETHSPGLEHVTFYQGGKAYGADLGPFKTPAREDDPRLMPPNFYYDQED
                     LLRGRQRGKAWHFTGLRPEAVSGNPMNLAMVIAVYAAISKELGLPLRFPGPEAAYRAL
                     YQLTSADILAQATDWAGSTPAARNEIFNITNGDYFRWQHLWPRIARMFGMETADPVPL
                     SLVEYMADKGPLWEEITRKYGLAPIPYDRLVSWAFGDFIFRSAFDNISSTIKARRAGF
                     HDCIDTEDMFAEFFAELHQNRVIPF"
     gene            1500..2633
                     /locus_tag="VIS74_01765"
     CDS             1500..2633
                     /locus_tag="VIS74_01765"
                     /inference="COORDINATES: protein motif:HMM:NF024770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEY5791993.1"
                     /translation="MKSQQPNLKLLRQAIHLDFQDDVPLHVQLRHGLSMAIQNYFQDG
                     QRFFNEKDIMEQLNVSQVTVRRALGDLVQERLLERRAAVGSFVRKRHLQEQSVDRVTL
                     LAPDWDSSFIISLIHECTRFCQLAGLQADTVLALQDGNMVGSSGNDQTLAPNIPLIAI
                     ALDPHFCLDLYHRFGNRRRIVNIDAWSENYPGPYVGTDFSQMIRIGLDHLQGLGHSRI
                     CLLVNENVECASVADSVKHFEAQCRRRKLRECHVVTCGTKTWESSHDKVFPMMPKVMA
                     LRPRPTAIFAFDDQGAWAALLWLARNGIKVPEEMSVLGFSDDRPSKYTYPPLTTIAQS
                     AREIAEIAVKMVGQKTISPLHHFLEPSLVVRESTAPPPDKKTA"
     gene            complement(2710..>3458)
                     /locus_tag="VIS74_01770"
     CDS             complement(2710..>3458)
                     /locus_tag="VIS74_01770"
                     /inference="COORDINATES: protein motif:HMM:NF012457.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HEY5791994.1"
                     /translation="AIRACSPQPAQVGFAPTMGAKVPATQSPADIEAARQVYFRLQSD
                     SIWGLSLWNDPVFLGKYPDEAPSVYGRNWPEIRAEDLELIAQPLDFVGYNCYTGDVIR
                     AGADGRPETLPYPPGNPRGSLDWLQMLPDALYWSVRFQVERYGRKPFVITENGLANLD
                     WVALDGKVRDPQRIDYMHRYLRGLKRAAEEGFPVGGYFYWSFLDNFEWAEGYQPRFGL
                     VHVDFETQARTLKESAAWYCEVIRTNGADL"
BASE COUNT          673 a         1146 c          991 g          648 t
ORIGIN      
        1 ccggccgggc gggaggagcc gccggaatcc cggatttcgg cggcaaagtg ccagccggtt
       61 cggttgcccc gtctccccgc cgcgggaaag ttggcccatg acgctcattg gaaacgacga
      121 tggaaaaacg cagaaccctc cctccaagac ggtcctcgtc gccggcgcgc acggggtgac
      181 cgggcgctac gccgcactgc attttgcccg gcaaaccgag gtggccgttt atggcttgtc
      241 gcggcgcttg gacgaattgc cgggcgttca ttttctgtcg gccgatctct tgaatctccc
      301 ggacgtcgaa gcgaagctgg cggggattcc cgccattacg cacctcgtgt tcgccgccta
      361 cgtcgagcgg ccgaccgcga aggaacggag cgaggtcaac acggcgctgc tgcgcaacct
      421 gctcgatggg ctggaaaccc attcccccgg cctggagcac gtcaccttct accagggcgg
      481 caaggcctac ggcgcggacc tcggcccctt caagactccc gcgcgcgagg acgatccgcg
      541 gctgatgcct ccgaattttt actacgacca ggaagatctc ctgcgcggcc ggcagcgcgg
      601 gaaagcctgg catttcaccg gcctccggcc cgaggccgtc tccggcaacc ccatgaacct
      661 ggccatggtc atcgccgtct atgccgccat ctcgaaggaa ctcggcctgc cgctccgctt
      721 ccccggaccg gaagcggcgt atcgcgccct atatcagctt acctccgccg acatcctggc
      781 ccaagccacc gactgggcgg gatcgactcc cgccgcgcgg aacgaaatct tcaacatcac
      841 caacggcgac tacttccgct ggcagcacct gtggccgcgg atcgcccgaa tgttcggcat
      901 ggaaaccgcc gacccggtgc cgctctcgct ggtggaatac atggccgaca aggggccgct
      961 ctgggaggaa ataacccgca aatacgggct cgcgcccatt ccctacgacc ggctggtctc
     1021 ctgggcgttc ggcgatttca tcttccgcag cgccttcgac aatatctcaa gcacgatcaa
     1081 ggctcgccgg gccgggtttc acgactgcat cgacaccgag gacatgttcg ccgaattctt
     1141 cgccgagctt caccaaaacc gggtgatccc attctaagag agggtgtacc gaaagccgga
     1201 attaaaccgt ggattacagg catggatggg cgcggattga tagtatttct atccgcagcc
     1261 atccgcgctg caacgcacgc gaagcggcgg accggattgg cgttttccta agcttctggg
     1321 agcgtggcat tcgggcaagg gcgggctatt tttagctctg gacaattcgc cgctccagcc
     1381 cgcattcttt gaaagttata atgtatggcg tgcttgcgac tctcgatgaa aaatgacccg
     1441 gccaaacctt ggaaaatgcg ggcatccccc acccccggca aaacggattt gagccagcca
     1501 tgaaaagcca gcagcccaac ttaaaactgc ttcggcaagc cattcacctt gattttcagg
     1561 atgatgtgcc gctgcacgtg cagttgcggc acggattgag catggccatc cagaattatt
     1621 tccaggatgg gcagcgtttc ttcaacgaga aggacatcat ggagcagctc aatgtttccc
     1681 aggtaaccgt gcggcgggcc ttgggcgact tggtgcagga gcgcctgctg gagcgccgcg
     1741 ccgccgtggg aagtttcgtc aggaaacgcc accttcagga gcagtccgtg gatcgcgtca
     1801 cgctgctggc cccggattgg gattcttcct tcatcatcag cctgatccac gaatgcaccc
     1861 gtttctgcca gctcgccggc ctgcaagccg acaccgtgct ggcccttcag gatggcaata
     1921 tggtgggctc cagcggaaac gaccagacgc tggcgccgaa tatccccttg attgccatcg
     1981 cgctggatcc gcatttttgc ctggatcttt accaccgctt tggaaaccgg cgccgaatcg
     2041 ttaacatcga cgcctggagt gaaaactatc ccggccctta cgtgggaacc gacttcagcc
     2101 agatgatccg catcgggctg gaccacctcc aggggctcgg ccactcgcgc atctgcctgc
     2161 tcgtcaacga gaatgtcgag tgcgccagcg tcgccgacag cgtcaagcat ttcgaagcgc
     2221 agtgccggcg ccgcaagctt cgcgaatgcc acgtcgtcac ctgcggcacc aaaacctggg
     2281 agtcctccca cgacaaggtt tttccaatga tgcccaaggt gatggccctg cggccccggc
     2341 cgacggcgat tttcgccttc gacgatcagg gagcctgggc ggcattgctc tggctcgccc
     2401 gcaatgggat caaggtgccc gaggagatgt ccgtcctggg attcagcgat gatcgcccca
     2461 gcaaatacac ctatcccccg cttaccacca tcgcgcagtc cgctcgcgaa atcgccgaga
     2521 ttgccgtgaa gatggtcggg caaaaaacga tctccccgct tcaccatttc ctggaaccct
     2581 cgctcgtcgt tcgcgaaagc accgccccgc cgccggacaa aaaaacggct tagcggagat
     2641 cttgtccgtc actgcccccg cgcctttgcg cctttgcgtg aggcctgccc tccttttttg
     2701 gagtggttct taaagatccg ccccgttggt gcggatgacc tcgcaatacc aggcggcgga
     2761 ctccttgagc gtccgagcct gcgtttcgaa gtccacatgc accaggccaa agcgcggctg
     2821 gtagccttcc gcccattcaa agttgtcgag gaacgaccag taaaaatatc cccccaccgg
     2881 gaagccttcc tccgccgcgc gcttgaggcc gcgcaaatac cgatgcatgt agtcgatccg
     2941 ctgggggtcg cgcaccttgc cgtccaacgc cacccaatcc agattcgcca gcccattttc
     3001 cgtgatcaca aacggcttgc gcccgtaacg ttcgacctgg aaccggacgg accaatagag
     3061 cgcgtcgggc agcatttgca gccagtccag gctcccccgg ggattgcccg gcggataagg
     3121 cagcgtctcc ggccggccat ccgcgcccgc gcgaatgacg tcgcccgtgt agcaattata
     3181 gccgacaaaa tcgagcggtt gggcgatcaa ttccagatct tccgcccgga tctccggcca
     3241 gttccgcccg taaaccgacg gcgcctcgtc gggatattta cccagaaaca ccggatcgtt
     3301 ccacaaggag agaccccaaa tcgagtcgct ctgcagccgg aaataaactt ggcgggccgc
     3361 ctcgatgtcc gccggcgatt gcgtggccgg aaccttcgcg cccattgtcg gggcgaatcc
     3421 cacctgcgcc ggctgcggac tacaggcgcg gatggctt
//
LOCUS       DATDAY010000091         4083 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_2301320, whole genome shotgun sequence.
ACCESSION   DATDAY010000091 DATDAY010000000
VERSION     DATDAY010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 4083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4083
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2301320"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1016
                     /locus_tag="VIS74_01775"
     CDS             <1..1016
                     /locus_tag="VIS74_01775"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEY5791995.1"
                     /translation="DRFSVAGLAGRDPAKTAAIARRFGITAHASVEELVRIGKPQFVV
                     TSVPWAANPSLLRRLAEMGMPALSETPPAGSIPEMEELCELARQGAKIQVAEQYFLQP
                     HHAARLAFIRGGKLGPVHQAQVSAAHGYHGVSLIRRFLGVGFENATIQAMICRSKMVE
                     GPGRSGLPAEERMAEAEQVIATLDFGGKVGVYDFTGRQYFALIRDPRVLIRGERGEIL
                     NQSAVYLRDFRTPIRADFVRHSAGQQGDLAGHYLAGIQAGEEWVYRNPFAPARLFDDE
                     IAIATCLEKMGQFAAGGEAFYPLAEACQDRYLDLMIAEAAAQGRRITTTTQAWAASNE
                     SHS"
     gene            1013..1846
                     /locus_tag="VIS74_01780"
     CDS             1013..1846
                     /locus_tag="VIS74_01780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5791996.1"
                     /translation="MKFKPHRTLSRKWPIISAFLGSLLLLVPAPQAIAQEGESVFGRR
                     ESSESALIGIIYDLKQTQQRHPSEINPNNYSQVVEEFLRKNWDESVLNRYFRSARPLY
                     TTQIFVPLMTADNAPKAFDMQAVIKPAVWLVHYKGQVAPPADGVYRFAGYSDDLIAVA
                     INDKTVCIGGRMDLDKVWKSPERRAGPSGANGSLTYGDWIPLKKDQPVDLDVIIGERP
                     GGQFCAFLLYEKQGASYPADASGKPLLPIFQLAPHDTPAQPPKLAPPFSPATETWKSF
                     Q"
     gene            1994..2839
                     /locus_tag="VIS74_01785"
     CDS             1994..2839
                     /locus_tag="VIS74_01785"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HEY5791997.1"
                     /translation="MLHFLRFPFRRSLLAAVIFGAGLSAGLAQAPTPDAAPPASAPAA
                     TPAEHLTAAAERSHRAQQEVGHRFSSGWGQRLKEGGTTALVQIGLSVFGAMFFFERIF
                     NLSRKKLVPNGLSRKARKLWKEGDFAALEALGKTDPSTLGRAIAWVAQHRHNSYADVS
                     VGAGDILSQEIEVHHQRAYPLGVVATLEPLLGLLGMILGMIQTFEVVALAGALGNPAQ
                     LAGGISEALITTGLGLAVAIPFLAFFHYFKSRTNMFSSQLEKEMTGLLSEWFLSKQPE
                     AVYES"
     gene            2829..3245
                     /locus_tag="VIS74_01790"
     CDS             2829..3245
                     /locus_tag="VIS74_01790"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEY5791998.1"
                     /translation="MKVKNRHSEQDFELPLIPMIDCLFVLLVFFLVATTLKKTDKELP
                     IDLPQSGAALDTEQKDDVLVIGLDRAGQRYFGSEPVTTEVMSQRLAAAALANPNRRVR
                     LDADVSTPYVAVVEMIELCEFNNLRNVGFRTKSPGK"
     gene            3211..>4083
                     /locus_tag="VIS74_01795"
     CDS             3211..>4083
                     /locus_tag="VIS74_01795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5791999.1"
                     /translation="MSASAPSLPENDGRSSALWLALSILVHSLIIGGLIFLVPIAELV
                     SPESESANNPAAEPPVSVSPERVQEIAKEIEATQAEEIRSKVEELLETQQSLAELEAQ
                     KQTDFSALAQEMAATAPQLAQDALAEATQAQAEAEKALAEALQAAEALKIAREAAAQP
                     ALTPEQQAAVNAQANTASSQTNSAQERAKNAQAKASAAQIIATQQLGFREEETFKEAR
                     TTQAAAVTAQNEANRKQDEAAALLDEQEALKRAANMAEFHRGAAERFVNLQRDRLGRS
                     ERDLNNTKSRGNLEA"
BASE COUNT          767 a         1429 c         1199 g          688 t
ORIGIN      
        1 cggatcggtt ctccgtcgcc ggtttggcgg ggcgcgatcc ggcgaagacc gcggcaatcg
       61 cccggcggtt cggcatcacg gcccatgcct cggtcgagga attggtccgg atcggaaagc
      121 cgcaattcgt ggtcaccagc gttccgtggg ccgcgaaccc cagcctcctc cggcggctcg
      181 ccgagatggg catgcccgcg ctctcggaaa ccccgcccgc cgggtcgatc ccggaaatgg
      241 aggagctgtg cgaactcgcg cgccagggcg ccaaaattca ggtggccgag caatatttcc
      301 tacaaccgca tcacgccgcc cgcctggcgt tcattcgcgg cgggaagctg gggccggtgc
      361 atcaggccca ggtctcggcg gcgcacgggt atcacggcgt cagcctcatc cggcggtttc
      421 tgggcgttgg tttcgagaat gcgacgatcc aggccatgat ctgccgctca aaaatggtgg
      481 aggggccggg ccggagcggc ctgccggcgg aggagcgaat ggccgaggcg gagcaggtca
      541 tcgccacgct ggatttcggc ggcaaggtcg gcgtttacga tttcaccggg cggcagtatt
      601 tcgccctcat ccgcgatccc cgcgtgctca tccgcgggga gcgcggggag atcctgaacc
      661 agtcggcggt ttatcttcgg gacttccgga cgccgatccg cgccgatttc gtgcgccaca
      721 gcgccgggca gcagggcgac ctcgccggcc attacctggc gggcatccag gcgggggagg
      781 agtgggtgta tcgcaatccc ttcgcgcccg cgcggttgtt cgacgacgag atcgccatcg
      841 ccacctgcct ggaaaagatg gggcagtttg ccgccggcgg cgaagcgttt tatccgctcg
      901 ccgaagcctg ccaggaccgt tatctcgatc tgatgatcgc cgaagccgcc gcccaaggcc
      961 ggcgcattac caccaccacc caagcctggg ccgcatcgaa cgagagccat tcatgaaatt
     1021 caaaccgcat cgaacccttt cccggaaatg gccgatcatt tccgctttcc tcggaagcct
     1081 gctcctcctc gttccggcgc cgcaggcaat cgcccaggaa ggtgaatccg tctttggccg
     1141 ccgcgaaagc agcgagtcgg ctttgatcgg cattatttac gatttgaagc agacccagca
     1201 acggcacccc agcgagatca atcccaacaa ttattcccaa gtcgtggagg aattcctgcg
     1261 gaagaattgg gacgaatccg tgctcaaccg atacttccgt tccgcccggc cgctctacac
     1321 cacccagatt ttcgttccgc tcatgacggc ggacaacgcc cccaaggcct tcgacatgca
     1381 ggcggtcatc aagcccgccg tctggctggt tcattacaaa ggccaggtcg ccccgcccgc
     1441 ggacggcgtg tatcgcttcg cgggctattc cgacgacctc atcgccgtgg cgatcaatga
     1501 caagaccgtc tgcattgggg gccgcatgga tctggataaa gtctggaaat cccccgagcg
     1561 ccgggccggt cccagcggcg ccaatggaag cctgacttac ggggattgga ttcccctgaa
     1621 aaaagaccag ccggtggatc tggatgtcat catcggcgag cggccgggcg gccagttctg
     1681 cgcctttctc ctgtatgaaa agcagggcgc cagctatccc gccgacgcgt ccggcaagcc
     1741 gctgctcccg atctttcaac tggcgcccca tgacaccccc gcccagccgc ccaagctggc
     1801 gccgcccttc tccccggcca cggaaacctg gaaaagtttc caatgatcgg cgggcgcccc
     1861 cattcccttc ttttcccatt ccccaggccg cgcttgccgt taaattcccc tttgcgccct
     1921 tgcaccgggc caaaaaatgt ataagaaagc accgatcccc ttgcgttacc tatcttcatt
     1981 gccacccacc ttcatgcttc atttccttcg tttcccgttt cgtcgcagcc tgctggccgc
     2041 cgtgattttt ggcgccggat tatccgccgg tctcgcgcaa gctcccactc cggatgccgc
     2101 cccgccggca agcgcccccg ccgccacccc ggcggagcac ctcaccgccg ccgccgaacg
     2161 cagccaccgc gcgcagcagg aagtcggcca ccgcttttcc tccggctggg gccagcggct
     2221 caaggaaggc ggcacgacgg cgctggtgca gatcgggctg tcggtctttg gcgccatgtt
     2281 tttctttgaa cgcattttta acctgagccg gaaaaaactg gtgccgaacg gcctctctcg
     2341 caaggcgcga aaactctgga aggaaggcga tttcgccgcg ctggaggccc tcggcaaaac
     2401 cgatcccagc acgctgggcc gcgccatcgc ctgggtcgcg cagcatcgcc acaactccta
     2461 cgccgacgtg tccgtgggcg cgggcgacat cctctcgcag gaaatcgagg tgcatcacca
     2521 gcgggcttat ccgctcggcg tcgtggccac cctcgagccg ctgctcggcc tgctgggcat
     2581 gatcctgggc atgatccaga ccttcgaagt cgtggcgctg gccggagcgc tgggcaatcc
     2641 cgcccagctc gccggcggca tctcggaggc gttgatcacc accggtctcg gcctggccgt
     2701 cgccattccg ttcctcgcct ttttccatta tttcaagagc cgcacgaata tgttcagctc
     2761 ccagctggaa aaggaaatga ccggcctgtt gagcgaatgg ttcctaagca agcagcccga
     2821 ggcggtttat gaaagttaag aaccggcact ccgaacagga cttcgagctg ccattgatcc
     2881 cgatgatcga ctgcctgttc gtgctgctgg tatttttcct ggtcgccacc acgctgaaaa
     2941 agaccgacaa ggagctgcca atcgacctcc cgcagtcagg cgccgcgctc gacaccgagc
     3001 agaaggacga cgtcctggtc atcggcctgg accgcgccgg ccagagatat ttcggctccg
     3061 agccggtcac cacggaagtc atgagccagc gcctggccgc cgccgccctc gccaacccga
     3121 accgccgggt ccggctggat gcggacgtga gcacgcccta cgtcgccgtc gtcgaaatga
     3181 tcgagctgtg tgaattcaac aacctgcgca atgtcggctt ccgcaccaag tctcccggaa
     3241 aatgacggcc ggtccagcgc tctctggctg gcgctgtcca ttctcgtcca ttccctgatc
     3301 atcggcgggc tgatcttcct cgtgcccatc gccgagctgg tttcgccgga atccgaatcc
     3361 gccaataatc cggccgccga accgcctgtc agcgtctccc cggagcgagt ccaggagatc
     3421 gccaaggaaa tcgaagcgac ccaggccgag gaaatccgct ccaaggtcga ggagctcctc
     3481 gaaacccagc aatccctcgc cgagctcgaa gcccagaagc aaaccgactt ttccgcgctc
     3541 gcccaggaaa tggccgccac cgcgccgcag ctggcgcagg acgccctcgc cgaggccacc
     3601 caggcgcagg ccgaggccga aaaagccctg gccgaagccc tgcaggccgc cgaagccctc
     3661 aaaatcgccc gcgaggccgc cgcccaaccc gcgctcacgc ccgagcaaca ggccgccgtc
     3721 aacgcgcagg ccaatacggc gtcctcccaa accaactccg cccaggaacg cgcgaagaac
     3781 gcccaggcca aggccagcgc ggcccagatc atcgccaccc agcagcttgg ttttcgcgag
     3841 gaggaaacct tcaaggaagc ccgcaccacg caggccgccg ccgtcaccgc ccagaacgaa
     3901 gccaaccgca agcaggacga ggccgccgcg cttctcgatg aacaggaagc gctcaaacgc
     3961 gccgccaaca tggccgaatt ccatcgcggc gcggccgagc gtttcgtgaa ccttcaaagg
     4021 gaccgccttg gacgcagcga acgggatctg aacaatacca agtcccgggg caacctggag
     4081 gcg
//
LOCUS       DATDAY010000092         2442 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_1294163, whole genome shotgun sequence.
ACCESSION   DATDAY010000092 DATDAY010000000
VERSION     DATDAY010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2442)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2442)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2442
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1294163"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            220..720
                     /locus_tag="VIS74_01800"
     CDS             220..720
                     /locus_tag="VIS74_01800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792000.1"
                     /translation="MLPAGTGGNHFAYIHPRGGGRSHYVSSIAGIQNRNSMKMHLFTR
                     TLLAAACAIALQGAAVHAQDATTTTVITSDGVVQQLVPASSEIMFLSPGSVTPVKYTF
                     NKTTTFVDAAGNTVTYESIKQNTPARIYYTKVGDSYVVTKFMVTEAPAVRKTEETTTT
                     TTTTPD"
     gene            725..916
                     /locus_tag="VIS74_01805"
     CDS             725..916
                     /locus_tag="VIS74_01805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792001.1"
                     /translation="MMTTHLLKFGAALVFLAAIGLSGCASSEPVTTTTTTRQVTTEPA
                     ATTVIQPAPTTVRQTTTTY"
     gene            1137..1910
                     /locus_tag="VIS74_01810"
     CDS             1137..1910
                     /locus_tag="VIS74_01810"
                     /inference="COORDINATES: protein motif:HMM:NF021391.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2092 domain-containing protein"
                     /protein_id="HEY5792002.1"
                     /translation="MKIPRLLAACLLSIFVTPAVLPAAEKSGPPPRIDPQALEGLQRM
                     STTLGGAKAFTYRSRSILEVPAATGQFLTLFSTAEVALKRPDKIRARLDGEAPGFDFY
                     YDGKTVSAFAPATKVYSVTPAPATIDAMLPDLEEETGIRFATAPLLFSDPYRVLTRRL
                     TSALVVGPAVVHGTACEHLAFRSPGVNWEIWIESGPRALPRRLAVTFTDRPNFPRTLV
                     EFSNWNLHPWLADGDFVFRKPAGAEAIPYLSVLKSTGRR"
     gene            1925..2362
                     /locus_tag="VIS74_01815"
     CDS             1925..2362
                     /locus_tag="VIS74_01815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792003.1"
                     /translation="MKNSLALLPMIALTAALSLSQTGCTVSSGPGYYGYYGPPTVVAW
                     NGAGFYGGTYYRSRSAYYGSPYYRGGYGSWNNGSGNAYGRRGGSAHWNDGSGSATGWR
                     GGSAHWNDGSGSATGWRGGSASWNDGSGSWSGARGRSGSWNRR"
BASE COUNT          440 a          824 c          706 g          472 t
ORIGIN      
        1 ccgccgtggc tgggtttgaa ggctgggtat gaccgatgtc ctcggttcat ccctcgaagg
       61 tttcgtcatg gccgccgtaa acgcggtccc gcttgagatt tgaggcgccc atcgagcggg
      121 gcgacgcgga ttgcccttca aaaaatggct gcgcggcgcg cggaactact ggacaaaatg
      181 ggggtaagcg ggatttcttc aaccggttta tcataataga tgctccccgc tggaaccggc
      241 gggaaccatt tcgcctacat ccatccccgc ggcggcggac gatcccatta cgtcagcagc
      301 attgctggca tccaaaaccg aaactctatg aaaatgcatc tcttcacccg caccctgctg
      361 gctgccgcgt gcgccatcgc gctgcaaggc gccgcggtcc atgcccagga tgcgaccacc
      421 accaccgtca tcacctcgga cggcgtcgtg cagcagttgg ttccggcttc gtcggaaatc
      481 atgtttctct cacccggctc ggtgacgcca gtgaaataca cgttcaacaa gaccacgacc
      541 ttcgtggacg cggctggcaa tacggtgacc tacgagtcca tcaaacaaaa tactccggcg
      601 cgcatctact acaccaaagt gggtgacagt tatgtggtga ccaaatttat ggttaccgaa
      661 gcgccggctg tccgaaagac cgaggaaacc acgaccacca ccacgacaac ccctgattaa
      721 tgccatgatg acaacccacc tgttgaaatt cggagctgcg ctggttttcc tggccgcgat
      781 tggcctttcc ggctgcgcca gcagtgaacc tgtaaccacc accaccacta cccgccaagt
      841 gaccaccgaa ccggctgcga ccacggtgat ccagccggcg ccaaccaccg tgcggcagac
      901 cacgaccact tattagccga tcatcgccac agttggtttg ggtgtggggg atgaaccgat
      961 ctccccggtt catcccttga aggtttcttc gttgccgcgc ttgcggcggc catggggaaa
     1021 tttaaggtgt caatcgggcg gctcaggctt aaatgaatgg ccggatgcca ctggcctgcg
     1081 cccaagatga gttcccctct tccctgccaa tcctcccatc aagcccacga ttttccatga
     1141 aaattccccg cctcctcgcc gcctgtttgc tctccatttt cgtgacgccc gccgtccttc
     1201 cggcggcgga aaaatccggt ccgccgccgc gcattgaccc ccaggcgctc gaagggctcc
     1261 agcgcatgag cacgacgctg ggcggggcga aggcattcac ctaccggtcg cgcagcatcc
     1321 ttgaagtgcc cgcggccacc gggcagttcc tcactttgtt ctcgaccgcg gaagtcgccc
     1381 tcaagcgccc ggacaagatc cgcgcccgcc tcgacggcga ggcgccggga ttcgattttt
     1441 actacgatgg gaaaaccgtc tccgccttcg cccccgccac caaggtttat tccgtgactc
     1501 cggctccggc gacgattgat gccatgcttc ccgatctgga ggaggaaacc ggcatccgtt
     1561 tcgcgacggc gcccctgttg ttcagcgacc cctaccgcgt cctgacccgc cggctcacga
     1621 gcgccctggt tgtcgggccc gccgtggtcc acggcaccgc gtgcgagcat cttgccttcc
     1681 gctcgcccgg cgtgaattgg gaaatctgga tcgagtcggg tccgcgcgcc ctgccgcggc
     1741 ggctggcggt gacgttcacc gaccggccca acttcccgcg cacgctggtg gagttttcga
     1801 actggaacct ccacccgtgg ctcgcagacg gggattttgt gtttcgcaaa cccgccgggg
     1861 ccgaagcgat cccctatctt tccgttttga agtccaccgg ccgccgctaa cccggaaacc
     1921 tgccatgaaa aactccctcg ccctgctccc gatgatcgct ctgacggcgg ctctttccct
     1981 ctcgcaaacc ggctgcaccg tcagttccgg acccggttat tacggctatt acggtccgcc
     2041 gaccgtggtg gcctggaatg gcgcgggctt ttacggcggc acttattacc gcagccgcag
     2101 cgcctactac ggcagcccgt attatcgcgg aggttacggt tcgtggaata acggctccgg
     2161 caacgcctat ggcaggcggg gtggctcggc tcattggaac gacggctcgg gcagcgccac
     2221 gggctggcgc ggcggctcgg ctcattggaa tgacggctcg ggcagcgcga ccggctggcg
     2281 cggcgggtcc gcctcctgga acgatggctc gggatcctgg agcggggcgc gcggacgctc
     2341 gggatcgtgg aaccggcggt agcggcatcc cgcttcgcgc cacttcacca gcgcccaacc
     2401 cgattttcgc aatgggtcca aaatgggggc tccggcgaca tt
//
LOCUS       DATDAY010000093         2897 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_1996634, whole genome shotgun sequence.
ACCESSION   DATDAY010000093 DATDAY010000000
VERSION     DATDAY010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2897)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2897)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2897
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1996634"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..507)
                     /locus_tag="VIS74_01820"
     CDS             complement(<1..507)
                     /locus_tag="VIS74_01820"
                     /inference="COORDINATES: protein motif:HMM:NF024968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY5792004.1"
                     /translation="MRKMEIEVVILNDYASVTGGSTSVALASALGLAARGVRVTLFTC
                     VGPVAPELIDVPNLEVICLDQPEIVKDTNRMRAFTSGLRNGGAIHALRALLADRDRRR
                     TVVHAHTWTKALSPFALNAVAEMGFPLVVTLHDFFITCPSGGFYIHGKGEICRRAPGS
                     ASCLVCNCD"
     gene            complement(507..1334)
                     /locus_tag="VIS74_01825"
     CDS             complement(507..1334)
                     /locus_tag="VIS74_01825"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY5792005.1"
                     /translation="MSITNRILVVTPTLGESAFLDETLQSVASQPLNILHVLAVPAGK
                     VEALKARYPGTIVVPDAGKEGAIYGALNAALNAVPEGWDWFTYINDDDLLLPGFGEMA
                     LRHFRRRRPEPVTYGDVELIDDAGGVLGHITVERSPKWIPALLQEGISPLMQQGMLFR
                     RDVVKRLVGFDQRYRLCADLDFWLRAYASGAAFRNYATRVARFRVRSGQLSGNTELTQ
                     REQDMIVARNLPIRRTAIRRALAVIRYRLANLPRYLARVRSRGFQTSYQILEGGGAA"
     gene            complement(1379..2548)
                     /locus_tag="VIS74_01830"
     CDS             complement(1379..2548)
                     /locus_tag="VIS74_01830"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEY5792006.1"
                     /translation="MKVLHIHAGIHYGGIQRIIATLARYAGLTPGLEQEFALCFNGRL
                     SRELDAFGARRHSLGPVRIRYIWQALRSRSALTAILRDGGHDAAVCHGSWALGVFGGA
                     IQRAGVPLVYWMHNDTPERRKNLVETVAAKTRPCLVIGNSQFTASSIPLAFDRIPPSE
                     VLPNPVDEPELAVPRAPLRKAVRRELGTDPDAVVILLAARPEAWKGHRLLMAALLRLA
                     DLGGWEAWIVGGAVNMEQAWLLAGLEREAADGGIANRVKFTGQRDDVPRIMAAADVFC
                     QPNLSPEPFGIVFAEALYAGLPVVSANHGGAREIVDDSCGILVSPGSIEKLAAALRKL
                     ILRPDLRGRLAANAPGRARELTSPRRVIPRMEALFRRFVPRRSKRSGERSTPVSR"
     gene            complement(2545..>2897)
                     /locus_tag="VIS74_01835"
     CDS             complement(2545..>2897)
                     /locus_tag="VIS74_01835"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEY5792007.1"
                     /translation="VVIGADSRFTGAKANIMIEDDFLSASGLYINTGSHDLDTLQPTY
                     LPVVIGRRVWCGMRVTICAGVSIGDNAVLGAGAVVLKNMPANHVAAGVPCKAIREIER
                     PDRGADADWTNFKR"
BASE COUNT          545 a         1022 c          842 g          488 t
ORIGIN      
        1 gtcgcaattg cagaccaggc acgaggccga gcccggcgcg cggcggcaga tctctccctt
       61 cccatggatg taaaatccgc cgctcgggca ggtgatgaaa aagtcgtgca gcgtcaccac
      121 cagcgggaat cccatttccg ccaccgcgtt caatgcgaag ggcgacagcg ccttcgtcca
      181 cgtatgcgcg tgcaccaccg tccggcggcg gtcccggtcc gcaagcaggg cgcgcagcgc
      241 gtggatcgcc ccgccattgc ggagcccgct cgtgaacgcc cgcatccggt tcgtatcctt
      301 cacgatctcc ggctgatcca ggcagatcac ctcgagattg ggaacgtcga tcagctcggg
      361 agccaccggt cccacgcagg tgaaaagcgt cacccgcacc ccgcgcgccg ccagcccgag
      421 ggcggatgcc agcgccacgg aggtcgatcc ccccgtcacc gacgcgtagt cgttcaggat
      481 caccacctcg atttccatct ttcgcatcac gccgccccgc ctccttccag aatctggtaa
      541 ctcgtttgaa aaccccgcga tctcacccgc gcgagatagc gcggcagatt cgccagccgg
      601 tatcgaatca ccgccagcgc ccggcggatc gccgtgcggc ggatcggcag gttccgggcc
      661 acgatcatgt cctgctcccg ctgcgtgagc tcggtgttgc cggagagctg gccgctgcgc
      721 acgcggaagc gcgccacccg cgtcgcatag tttcgaaaag cggcgccgct cgcatacgcc
      781 cgcagccaga aatccaggtc cgcgcaaagc cggtaacgct ggtcgaatcc caccagccgt
      841 tttacgacat cgcggcggaa caacatcccc tgctgcatca gcgggctgat tccttcctgc
      901 aaaagcgccg gaatccactt cggggaacgc tcgaccgtga tgtgcccgag cactccgccc
      961 gcatcgtcga tcaattccac gtccccatac gtcaccggct ccgggcgccg gcggcggaaa
     1021 tgccgaagcg ccatctctcc gaagccaggc agcaacaggt cgtcgtcatt gatgtaggtg
     1081 aaccaatccc atccctccgg cacggcattc agcgcggcat tcagcgcgcc gtagatcgcg
     1141 ccttcctttc cggcatccgg cacaacgatc gtccccggat agcgggcttt cagggcttcg
     1201 acttttcccg ccggcaccgc gaggacatgc aaaatgttca gcggctgcga tgcgacggat
     1261 tgcagcgttt catcgagaaa cgcggattct cccaacgtgg gagtgacgac caggatgcga
     1321 ttcgtgatgc tcataggatt gagaaatgcg tgtcaggaca tgttgttgat ggtggttttc
     1381 atcgggaaac aggtgttgat cgttccccgc ttcgtttgct ccgcctgggc acgaacctgc
     1441 gaaaaagcgc ctccatccgc ggaatcaccc gccgcggcga cgtgagttcc cgggcccgcc
     1501 caggcgcatt ggcagccagc cgcccgcgca aatcaggtcg gaggatcagt ttgcgtaacg
     1561 cggcggcgag tttctcgatc gaccccggcg aaaccaggat gccgcacgag tcatcgacga
     1621 tctcgcgcgc gccaccgtgg ttcgcactca ccacgggcag ccccgcatag agggcttcgg
     1681 cgaagacgat cccaaaaggc tccggcgaca ggttgggctg acaaaaaaca tccgccgccg
     1741 ccatgatccg gggcacatcg tcccgctgcc ccgtaaattt cacccggttg gcgataccgc
     1801 cgtccgcggc ctctcgctcc aggcccgcaa gcagccacgc ctgctccata ttcacggccc
     1861 ccccgacgat ccacgcctcc cagccgccca gatcggccaa tcgcagaagg gccgccatga
     1921 gcaggcggtg ccctttccat gcttcggggc gggccgcgag caggatcacc accgcatccg
     1981 gatcagtgcc cagctcccga cgcaccgcct tgcgcaacgg cgcgcggggc acggccagtt
     2041 cgggctcatc gacgggattc ggcaggactt ccgaaggcgg aatccggtca aatgccagtg
     2101 gaatcgatga tgccgtaaac tggctgttgc caatgacgag gcaggggcgg gtcttcgccg
     2161 caaccgtttc gacgagattc tttctgcgtt cgggagtgtc attgtgcatc caatacacca
     2221 gtggcacccc ggccctttga atcgctcctc cgaacacacc gagcgcccac gaaccatgac
     2281 aaaccgccgc gtcgtgcccg ccgtcgcgta atatcgccgt cagcgcgctt ctcgaccgca
     2341 acgcctgcca gatgtaacga atcctcaccg gccccaggct gtgccgcctc gctccgaagg
     2401 catcgagctc gcgggagagg cggccgttaa aacaaagcgc gaactcctgc tccagccccg
     2461 gcgtcagccc ggcataccgc gcaagcgtcg cgatgatccg ctggatgccc ccgtaatgaa
     2521 tcccggcgtg gatgtgcagc actttcatcg tttgaaattc gtccaatccg catccgcgcc
     2581 acggtcgggg cgctcgatct cgcggatcgc cttgcacggc accccggcgg ccacgtgatt
     2641 ggccggcatg tttttcaaga cgaccgcccc cgcgccgagg acggcgttgt ctccaatgct
     2701 gacgccggcg cagatcgtca cgcgcattcc gcaccacacg cggcgcccga tgaccaccgg
     2761 cagataagtc ggctggagcg tatccagatc atgcgagccg gtattgatgt agagcccgct
     2821 cgccgagaga aaatcgtctt caatcataat attcgccttc gcgcccgtga acctcgagtc
     2881 ggcgccgatg accaccc
//
LOCUS       DATDAY010000094         2768 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_1867996, whole genome shotgun sequence.
ACCESSION   DATDAY010000094 DATDAY010000000
VERSION     DATDAY010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2768)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2768)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2768
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1867996"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            260..1519
                     /locus_tag="VIS74_01840"
     CDS             260..1519
                     /locus_tag="VIS74_01840"
                     /inference="COORDINATES: protein motif:HMM:NF022784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3142 domain-containing protein"
                     /protein_id="HEY5792008.1"
                     /translation="MRAGLLLKLFALLPLLSACSRPAPLQNGVYVWQQKPDRAVGNAI
                     ERAAPRMDIFYLHAADWVFRNNQATTSRFEPDWQAIRASKKPAGLVLRISFAQGGLGS
                     SPRHAPDVIRLGRSLIDSTRSAGIACGEFQIDYDCPESKLAIYAAFLKEIQSSLPDVS
                     LTFTALPSWLKHPEFKQLARVTNRHVLQVHSLELPNKDDSTATLCDPSKALTAIDAAS
                     RIGVPFWVALPTYRCLVVLNEAGERIEIISENLSSMPAEGARVIPGAAVPGELAALMA
                     RLRLHPPKGLLGVVWFRLPVDTDRMNWSWPTLQAVMDGRVPESRLRVTANSNGMGTAT
                     VEIQNFGEQGEDLPALVQVRWQSGTLVAADGLKDYTMTVEPGKRLCSFRHNKTKVPFP
                     ELPPGEKRTIGWIRTSQPEPLEVEMIR"
     gene            1586..>2768
                     /locus_tag="VIS74_01845"
     CDS             1586..>2768
                     /locus_tag="VIS74_01845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792009.1"
                     /translation="MTLWHWRGLFGLILGQLTAVTLNVNACGPFFPESVLDQPKAVLR
                     PPFVNFEAEVRQLSPPSPPPAVAPPLPESAENSDPPYRFVDDDLLKPEFDEMRQILSS
                     LPPPRRGQLFDEYHSLRSAMIAKFPPANFETFSRWRQPENYEILANAELGKWPEGLPG
                     EIVLYLEGALQYHYGNRPAAIEIWKRLLALPPKERRNRSTWAAWMIAKATREESGLAA
                     AAPWYEECIRLSSEGYSDALKLGLTSLGWLARQELQQGHRRRALELYYHQARAGDVRG
                     WSSLRQSLPDPEKLSDAELIEMAADPFQRGLLTAELLGGNFDWHRFSGDSDSPQTPSI
                     VRWLNALEHANAGNLAEAARWAELAYSDGQFDLAKRWLARAPESDPRALWIRGKLALM
                     EG"
BASE COUNT          562 a          868 c          783 g          555 t
ORIGIN      
        1 cgaacgaccg ggctgctgag gatcacctcc ggcgcgaagc cattgcgcgc gcagaaccgg
       61 cccacctttt ccgcctgttc gatccccttg gccgtcaggt ggcggtgata atcgtccgcg
      121 caatgcggct ccgcctcggc gtgccggagg aaatagagca tcatgagagg gtaatcttaa
      181 cgcttcccgc gaaaaaaacg caagccgccc cgcgagattg ccggaacccg aaacaaacgc
      241 taccaaggaa ggcgactcca tgcgcgcggg cctattgctt aaattatttg cgttgctgcc
      301 tctgctttcc gcctgttccc ggccagcgcc cctgcaaaat ggcgtatatg tctggcagca
      361 aaagccagat cgcgcggtcg gcaacgccat cgaacgagcg gccccgcgga tggatatctt
      421 ctatctgcac gcggctgatt gggtgtttcg gaacaaccag gccactacaa gccgcttcga
      481 gccggactgg caagccatcc gcgcctcaaa aaagcccgcg ggattggtgt tgcggatttc
      541 cttcgcacaa ggcgggcttg gcagttcgcc ccggcatgcg ccggacgtaa tcagacttgg
      601 ccggtccttg attgattcca cccggagtgc cgggattgcc tgtggggaat tccagatcga
      661 ctacgattgt cccgagtcca agctggcgat ctacgccgcc tttctgaagg aaatccaatc
      721 ctcccttccg gatgtttccc tcaccttcac ggcgctcccg agctggctca agcatcccga
      781 gttcaagcag ctggcgcgcg tcaccaaccg tcacgtgctc caggtgcatt ccctggagct
      841 tcccaacaag gacgattcaa ccgccaccct ttgcgatcct tccaaggcgc tcaccgccat
      901 tgatgccgcc agccggatcg gcgttccgtt ctgggtcgca ctccccacct accgttgttt
      961 ggtggttctc aatgaagccg gcgaacgaat cgaaatcata tccgaaaacc tctcttcaat
     1021 gccggctgag ggcgcgcggg ttatccccgg agcggccgtc cccggagagt tggcggcatt
     1081 gatggcgagg ctgcgccttc atccgccgaa aggattgctt ggagtcgttt ggttccgcct
     1141 tcctgtggat accgaccgga tgaattggag ctggcccacc ctccaggcag tcatggacgg
     1201 ccgggttccg gaatcccgtt tgagagtcac cgcaaactcc aatggcatgg ggacagccac
     1261 cgttgaaatt cagaacttcg gcgagcaagg cgaagacctt cccgcactcg tccaggtgcg
     1321 ctggcaaagc ggaacattgg tcgccgcgga tggattaaaa gactacacga tgactgtgga
     1381 acccggaaaa cggctttgct cctttcggca caacaagacc aaagtcccct ttcccgagct
     1441 gccaccgggt gaaaagcgaa cgatcggctg gattcgcacc agccagccgg aaccgctcga
     1501 agttgaaatg attcgttagg aaatcgtgaa ccccttgcct ggatggcctt cctttcagtt
     1561 ggaattaatg ccaccatcgg aaaaaatgac gctctggcac tggcgcgggt tattcggatt
     1621 gatcctcggt cagttgaccg ccgtcaccct caacgtcaac gcctgcggac cctttttccc
     1681 ggaatcggtt ctcgaccagc ccaaagccgt gcttcgcccg ccgttcgtga atttcgaagc
     1741 ggaagtccgc cagttatctc ctccctctcc accaccagcc gtcgctcccc ctttaccgga
     1801 gtcagcggaa aacagcgatc cgccatatcg cttcgtggac gatgacctgc tgaaaccgga
     1861 attcgacgag atgcggcaaa ttctttccag ccttcccccg ccgcgtcgcg ggcagctttt
     1921 cgacgaatac cattcgcttc gttcggcaat gatcgcgaag tttccccccg cgaacttcga
     1981 gactttcagc cgttggaggc agcccgaaaa ttacgaaatt ttggcgaatg ccgaactcgg
     2041 caaatggccg gagggactcc ccggagagat cgttctttat ttggaggggg cgctgcaata
     2101 ccactacgga aaccgtccgg cggcaatcga aatctggaag cgtctgcttg ccttgccgcc
     2161 gaaggagcgg agaaatcgct cgacctgggc ggcttggatg atcgccaaag ccacccgcga
     2221 ggaatccgga ctggccgcgg cagcgccctg gtatgaagaa tgcatccggc tctcgtccga
     2281 aggatattcc gatgcgctga aattgggact gacctcattg ggatggctgg cccgccaaga
     2341 gctgcagcaa ggacatcgcc gccgcgcgtt ggagctttat taccatcaag cccgcgcggg
     2401 cgatgtgcgc ggatggtcct cgctgcgcca gtcgctcccg gatcccgaga aactttccga
     2461 cgccgagctt atcgaaatgg ccgccgaccc atttcaacgt ggactcctca cggcagaatt
     2521 gctgggtgga aattttgact ggcatcgttt cagcggtgat tccgattccc cgcaaactcc
     2581 gagcatcgtc cgctggctga acgcgctgga acacgccaac gccgggaatc tcgcggaagc
     2641 cgcccgctgg gcggaactcg cctattcaga tggccaattc gacttggcga aacggtggct
     2701 ggcgcgggct ccggaaagcg atccgcgggc tttgtggatt cgcggcaagc ttgcgctcat
     2761 ggaaggcc
//
LOCUS       DATDAY010000095         3520 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_302344, whole genome shotgun sequence.
ACCESSION   DATDAY010000095 DATDAY010000000
VERSION     DATDAY010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 3520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3520
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_302344"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..513
                     /locus_tag="VIS74_01850"
     CDS             <1..513
                     /locus_tag="VIS74_01850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792010.1"
                     /translation="RATMIQLRDAQAQIATLQATQVENEQKITDLTAQLETLANQAAA
                     DQQTAKKTIDDLNARITEQDTKLAQLIEALGKWKKAYNQAADLARAKENERAKLANKV
                     ILLDRRIADQQTKNDALYQIGNEILTRYEKFSLGDALTAREPFVGITRVKIQNLVQDY
                     QDKLTDQKIQ"
     gene            510..1442
                     /locus_tag="VIS74_01855"
     CDS             510..1442
                     /locus_tag="VIS74_01855"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEY5792011.1"
                     /translation="MMKTSILSSVSARARRVIFGLAFSGALAGTALAADAEALARSGG
                     AEITLEDVRPLLDNLSPRDQTALARDPAPLNQLVRSLLVQQLLLKEALASQWDQRPEV
                     AAQLERARQNLIAESYLQSISKPAAGFPTETDLQAAYQANKASLLVPRQLRLAQIYIA
                     VPKNADKEALAAAQAQLDATSKSLKQPNADFASIARTSSDEPASAERGGEIGWLAESQ
                     IQPEIRTKITALAKNGVSEPLRLADGWHILKVLDLKEAYTPALEEVRDSLAQQLRAAR
                     TRANSEAYLAKLLQQNPVAINEIALAQLLSGAKK"
     gene            1509..>3520
                     /locus_tag="VIS74_01860"
     CDS             1509..>3520
                     /locus_tag="VIS74_01860"
                     /inference="COORDINATES: protein motif:HMM:NF017658.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="filamentous hemagglutinin N-terminal
                     domain-containing protein"
                     /protein_id="HEY5792012.1"
                     /translation="MPRVEIPHRSFATPRRTRRLAVPAIAGALLLLAVNFPVDAGDIL
                     RGGASMSSSRKNTSTRTNAGAAAAAEAKANAQDRLARTTQALQAVKAMQSTARGLARP
                     QVNLGIDPNHPGRPLPNVPNGLRPGGLDIQGAPVGADAPVQSGSGGKINVEIKQNQQR
                     ALLNWKTFNVGKDTTLTFDQSAGKADRGQWIAFNKVRDPSGSPSQILGAIKADGQVYV
                     INQNGIIFGGASQVNVHALVASSLPVNDNLIDRGLLNNPDAQFLLSALAIPTGAKGTP
                     AFTPPAPLTPDGKIGDVVVQAGAQLSSPTSAANIGGRIALVGANVRNEGTISTPDGQT
                     ILAAGLQVGFTSHPGSDPTLRGLDVYVGKVGNYGGTAANAGLIDAPRASVVMTGKNVN
                     QLGVIDSSTSVTLNGRIDLLADYDAAPNLEENIFNSFSTGGVRNLDPYLFLPRSAGTV
                     TLGKNSVTRILPEIGSTQTVVGTELPLRSQVNLQGKDVHFRTKAMLLAPNAEVRVGAG
                     RWAFDGSDPTQPKVNFVYSGGQVYLDAGAFLDAAGTPDVSVSILQNILKLQLRGAELA
                     DSPLERESVLRAIDLIVDARENGVYDGRFWIGTPLGDVTGFAGLIQRTAAQLTTAGGN
                     VEIQAGGAVVLQKGSAVDVSGGYISYQGGEVRTTRIIDGARVIDIS"
BASE COUNT          689 a         1297 c         1019 g          515 t
ORIGIN      
        1 cgggcgacga tgatccagct ccgcgacgcc caggcccaga tcgccaccct gcaggccacc
       61 caggtggaga acgaacaaaa aatcacggac ctcaccgcgc aattggagac cctcgccaat
      121 caggcggccg ccgaccagca gaccgcgaag aaaaccatcg acgacctgaa tgcccggatc
      181 accgagcagg acacgaagct cgcccagctc atcgaagccc tcggcaaatg gaagaaagcc
      241 tacaaccagg ccgccgacct ggcccgcgcc aaggaaaacg aacgcgccaa gctggcgaat
      301 aaagtcattc tcctcgaccg ccgcatcgcc gaccagcaga ccaagaacga cgccctgtat
      361 caaatcggca acgaaatcct cacccgttac gaaaaattca gcctcgggga cgccttgacc
      421 gcccgggagc ccttcgtggg catcacgcgc gtcaaaatcc agaacctcgt ccaggactac
      481 caggacaaat tgactgacca aaaaatccaa tgatgaaaac ttccattctc tcttccgttt
      541 ccgcgcgcgc gcggcgggta attttcggct tggcgttttc cggcgcgctg gccggcaccg
      601 ctttggcggc cgatgccgag gcgctcgccc gcagtggcgg cgccgaaatc accctcgagg
      661 acgtccgtcc gctgcttgac aacctcagcc cgcgcgacca gaccgccctg gcccgcgacc
      721 cggccccgct caaccagctc gtgcgttcgc tcctcgtcca acagctcctg ctcaaggaag
      781 ccctcgccag ccagtgggac caacggccgg aggtggccgc gcaactcgaa cgcgcccgcc
      841 agaacctcat tgccgaaagt tacctgcaat ccatctcgaa gcccgccgcc ggtttcccca
      901 ccgaaaccga cctgcaggcc gcctaccagg ccaacaaggc gtcattgctc gtgccgcgcc
      961 agctccgcct cgcgcagatt tacatcgccg ttccgaagaa cgcggacaag gaagccctgg
     1021 cggccgccca ggcccagctc gacgccacct cgaaaagcct caagcagccg aacgccgact
     1081 tcgccagcat cgcccgcacg agcagcgacg agccggcgag cgccgagcgc ggcggagaaa
     1141 tcggctggct ggcggaatcc cagatccagc ccgaaatccg cacgaagatc accgccctcg
     1201 cgaagaacgg cgtctccgaa cccctgcggc tggccgatgg ctggcacatc ctcaaagtcc
     1261 tcgatctcaa ggaagcctat accccggctc tcgaggaagt ccgggactcg ctcgcccagc
     1321 agctgcgcgc cgcccgcacc cgcgccaaca gcgaagctta cctcgccaag ctgctgcaac
     1381 agaacccggt tgcgatcaac gaaatcgccc tcgcccaact cctgagcggg gcgaaaaaat
     1441 aacgatgccg gggaaacgcc tcactccaac cggccggttt ccggctggcc gctcgtaacg
     1501 cccgctccat gccccgcgtg gaaattccgc atcgatcctt cgcaacgccc cgccgcaccc
     1561 ggcggctggc ggtcccggcc attgccggcg cgctcctgct gctcgcggtc aattttccgg
     1621 tggatgcggg cgacatcctc cgcgggggcg cctcgatgtc gtcgtcgcgg aagaacacct
     1681 ccacccggac caatgccggg gccgcggccg cggcggaggc gaaggccaat gcccaggacc
     1741 ggctggcccg caccacccag gcgctccagg cggtgaaagc gatgcagtcc acggcccgcg
     1801 gccttgcccg gccgcaggtc aacctgggaa tcgatcccaa ccaccccgga cgcccgctgc
     1861 ccaatgtgcc caatggattg cggcccggcg gactcgacat tcagggggcg ccggtgggcg
     1921 ccgatgcccc cgtgcagtcg ggaagcgggg ggaagatcaa cgttgaaatc aagcaaaacc
     1981 agcagcgcgc gcttttgaat tggaagacct tcaacgtcgg aaaagatacc acgctcacct
     2041 tcgaccagag cgcgggcaag gccgaccggg gccaatggat cgccttcaac aaggtccgcg
     2101 acccctcggg ctcgccctcg caaattctgg gcgccatcaa ggcggacggc caggtctatg
     2161 tgatcaacca gaacggcatc atcttcggcg gagccagcca ggtgaatgtc cacgcgcttg
     2221 tcgcgtcctc cctgccggtc aacgacaacc tcatcgaccg cggcctgctc aacaaccccg
     2281 acgcccaatt tctcctctcc gccctcgcca ttcccactgg cgccaagggc actccggcct
     2341 ttacgccgcc cgccccgctg acgccggatg gcaagatcgg cgatgtcgtc gtccaggctg
     2401 gcgcccagct cagcagcccg acttccgcgg ccaatatcgg cgggcgcatc gccctcgtgg
     2461 gggcgaacgt ccgcaacgag gggacgattt ccactccgga tggccagacg atcctcgccg
     2521 ccggcctgca ggtcggtttc acctcccacc cgggcagcga tcccaccctg cgcgggctcg
     2581 atgtgtatgt cggaaaagtc ggcaattacg gcggcacggc ggccaatgcc ggcctcatcg
     2641 acgcgccccg cgccagcgtc gtcatgacgg gtaaaaacgt caatcagctg ggcgtcatcg
     2701 acagctccac ctccgtcacg ctcaatggcc ggatcgacct gcttgccgat tacgatgccg
     2761 ctcccaatct ggaggaaaac atcttcaact ccttctccac cggcggcgtc cgcaacctcg
     2821 atccctacct gtttcttccc cgatccgcgg gcaccgtgac cctcgggaaa aacagcgtca
     2881 cgcgcatcct tcccgagatc ggcagcaccc agacggtcgt cggcaccgag ctgcccctgc
     2941 gttcccaggt caacctgcag ggcaaggacg tccacttccg gaccaaggcc atgctcctgg
     3001 cgcccaacgc ggaggtccgc gtcggcgcgg gccgctgggc tttcgacggt tcggacccca
     3061 cccaacccaa ggtcaatttt gtgtattccg ggggccaggt ctatctggat gccggcgcct
     3121 tcctcgacgc cgccggcacg ccggacgttt ccgtctccat tttgcagaac atcctgaaac
     3181 tccagctgcg cggcgcggag ctggcggact cgcccctgga gcgggagagc gtgctgcggg
     3241 cgatcgacct gattgtcgat gcccgggaga acggcgttta cgacgggcgt ttctggatcg
     3301 gcaccccgct gggcgacgtg accggtttcg cgggcctgat ccagcgcacc gccgcccaac
     3361 tgaccaccgc cggcggcaac gtggagatac aggcgggcgg agcggtcgtc ctccaaaaag
     3421 gttccgccgt cgatgtctcg ggcggctaca tcagctacca gggcggggaa gtccggacga
     3481 cgcggatcat cgatggcgcg cgggtcatcg acatttccga
//
LOCUS       DATDAY010000096         2964 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_1350648, whole genome shotgun sequence.
ACCESSION   DATDAY010000096 DATDAY010000000
VERSION     DATDAY010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2964)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2964)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2964
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1350648"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..253)
                     /locus_tag="VIS74_01865"
     CDS             complement(<1..253)
                     /locus_tag="VIS74_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CbiX/SirB N-terminal domain-containing protein"
                     /protein_id="HEY5792013.1"
                     /translation="MTARPHAALVLVGHGSTLNPDSSAPTLDHADAIRARGLFAEVVS
                     CFWKEEPSMREVYRMVESGEIYIVPTFISEGYFTRTVIPR"
     gene            complement(250..1746)
                     /locus_tag="VIS74_01870"
     CDS             complement(250..1746)
                     /locus_tag="VIS74_01870"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEY5792014.1"
                     /translation="MNKLFSPSLAMWLLLASSGLGADALNLTLADAVRRALAKNFLIR
                     MEEFTPKIARAQQKSTAGTFDPTAQLSYNYNYQEQAGIAVDPISGNPANVDLVAISSG
                     QVMDGSLSGLLPTGLRYDLGATLNVDDTSNRSYTRYNSFVGASIIQPLLRGFGTDVNL
                     GALRIARANVTISQWQLRQRVMDVITQTVQVYNDLYFFIRNLEVEERTRDLAAQLLKD
                     NMKRAEVGVMSPLDVVQAQADLAAREERVLVAERQMKDTENALKQLVTDNVSDVLSLQ
                     LSIVPPAANLDYKVDRTRDFAAAFELRPDYRQALLDLQKRNINLVFARNQILPRLDLL
                     ASLGLNGIDTGLSDSVLGTLSENNALSASIGGVVSVPIPNQYSRGQLDVAKLEIARAL
                     VDLKRIEQGILVESDNAAGAIETSRKRIDASRSARDYAAQTLDAAQTRLISGTSTTFE
                     VLQFQRDLANAEISEVGALTDFNKAIAEYARRTGTTLRRYQIVAPRTP"
     gene            complement(1739..2677)
                     /locus_tag="VIS74_01875"
     CDS             complement(1739..2677)
                     /locus_tag="VIS74_01875"
                     /inference="COORDINATES: protein motif:HMM:NF013705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HEY5792015.1"
                     /translation="MAVKFKDYYEILGVSKGASEEEIKSAFRKLARKYHPDVATDKKT
                     AEEKFKELNEAYEVLSDPAKRQKYDAYGQDWEHAGQGFPPPGGGAYSGGFPGGGATEF
                     EFGGTGFSDFFEQLFGSRRGTARAGGFGGFDFEESPPRGQDIETDILVTLEEAFHGST
                     RQISFRRAHGPKAETYTVRIPKGVRDGQRIRLAGIGGAGASGGAAGDLYLRVKIEQHP
                     DYELRGSDIYHDLEIPAYQAVLGGEVQAATLEGLAKLKIPPGTQSGQKFRLSGRGMPG
                     KGGGRGDFYVVIRPLLPKHVPAAERELWEQIRKLHE"
     gene            complement(2680..>2964)
                     /locus_tag="VIS74_01880"
     CDS             complement(2680..>2964)
                     /locus_tag="VIS74_01880"
                     /inference="COORDINATES: protein motif:HMM:NF013097.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HEY5792016.1"
                     /translation="YYSFATQFIVAFGVAFEMPIAVLVLVKLGIVDVRLLRRTRPFAV
                     ILILIFSAAVTPTQDMLTLLLMAGPMYVLYEICILLARFFPRRGGDLEDA"
BASE COUNT          536 a          937 c          945 g          546 t
ORIGIN      
        1 cgcgcgggat gacggtccgg gtgaaataac cttcgctgat gaacgtgggc acgatgtaaa
       61 tttccccgct ttccaccatt cgatacactt cgcgcatcga aggctcctcc ttccaaaagc
      121 agctcaccac ctcggcgaaa agcccgcggg cgcgaatggc atccgcgtga tccagcgtgg
      181 gggcgctcga gtcgggattc agcgtcgagc cgtggccgac gagcaccagg gcggcatgcg
      241 gccgggcggt catggcgtgc ggggggccac gatctggtaa cgccgcaggg tcgtgccggt
      301 gcggcgcgcg tattcggcga tcgccttgtt gaaatccgtc agcgcgccca cctcgctgat
      361 ctcggcattg gcgagatcac gctggaactg gagcacttca aacgtggtgc tcgtgcccga
      421 aatcaggcgc gtctgcgcgg catccagcgt ttgggccgcg tagtcgcggg ccgagcggga
      481 ggcgtcgatc cgcttgcggc tggtttcgat cgcgccggcc gcattgtcgg attcgacgag
      541 gatgccctgc tcgatccgct tgagatccac cagggcccgc gcgatttcga gcttggccac
      601 gtcgagctgg ccgcgcgaat attgattggg aatcggcacg ctcaccacgc cgccgatgct
      661 ggcggaaagc gcgttgttct ccgacaaggt gccgagcacg ctgtcgctca ggcccgtgtc
      721 aatgccgtta agcccgaggc tggccaggag atcgagccgc ggcaggatct gattgcgggc
      781 gaagacgaga ttgatgttgc gtttctgcaa gtccagcagg gcctgccggt agtcgggccg
      841 gagctcaaac gccgccgcaa aatcgcgcgt ccggtccacc ttgtaatcca gattcgccgc
      901 gggcggaacg atgctgagct ggagcgagag cacgtcgctg acgttgtcgg tgaccaattg
      961 cttcagcgcg ttttccgtgt ccttcatctg ccgttcggcg accaggacgc gctcctcgcg
     1021 cgccgccaga tcggcctggg cctggacgac gtcgagcggc gacatgacgc cgacttcggc
     1081 gcgcttcatg ttatccttga gcaattgcgc ggcgagatcg cgcgtgcgtt cctccacctc
     1141 gagattccgg atgaaaaaat aaaggtcgtt gtaaacctgc accgtctgcg tgatgacatc
     1201 catgacgcgc tggcggagct gccattggga aatggtgaca ttcgcccggg cgatgcgcag
     1261 cgcgcccagg ttcacatccg tgccaaagcc gcgcagcagg ggctggatga tcgaagcgcc
     1321 gacgaagctg ttgtagcggg tgtaggagcg gttggaggtg tcgtccacgt tgagcgtcgc
     1381 gccgagatcg taacgcaggc cggtgggaag caggccggag agcgagccgt ccatcacctg
     1441 gccggaggaa atcgcgacca aatccacgtt ggctggattt cccgaaatcg gatccaccgc
     1501 gatgccggct tgttcctggt aattgtaatt gtaggaaagc tgcgcggtcg gatcaaaggt
     1561 gcccgccgtg gacttctgct gggcgcgcgc gatcttcgga gtgaactcct ccattcggat
     1621 caggaaattt ttggccagcg cgcgccggac ggcatcggcc agcgtcagat tcaaggcatc
     1681 cgcgcccaaa ccgctggatg ccagcagcag ccacatcgcc aggctggggg aaaaaagttt
     1741 attcatgcaa cttgcggatc tgctcccaga gctcccgctc cgcggccggg acatgcttcg
     1801 gcaaaagggg ccggatcacc acataaaaat cgccgcgccc gccgcctttt cccggcatgc
     1861 cgcgcccgct gaggcggaac ttctggccgg attgcgttcc gggcggaatc ttcaatttcg
     1921 ccaggccctc cagcgtggcc gcctgcactt cgccgccaag cacggcttga taggcgggga
     1981 tttccagatc gtggtaaata tccgatccgc gcagttcgta gtcagggtgc tgttcgattt
     2041 tgacgcgcag gtataagtcg cccgccgcgc cgcccgaggc gcccgcgccg ccaatgccgg
     2101 ccaggcggat gcgctgccca tcccgcaccc ccttgggaat gcgcacggtg taggtctccg
     2161 ccttcgggcc atgggcgcgg cggaaggaaa tctgccgggt ggacccgtgg aaggcttcct
     2221 ccagggtgac caggatgtcc gtctcgatgt cctggccgcg cggcgggctt tcctcgaagt
     2281 cgaatccgcc gaagccgccc gcccgggcgg ttccccggcg ggatccaaac agctgctcga
     2341 agaagtcgct gaaacccgtg ccgccgaatt cgaactcggt cgcgccgccg ccgggaaacc
     2401 cgccggaata cgcgccgccg cccggcggcg gaaatccctg gccggcgtgc tcccaatcct
     2461 gcccgtaggc gtcgtatttc tggcgcttgg ccggatcgct caacacctcg taggcctcgt
     2521 tgagctcctt gaatttttcc tccgccgtct ttttgtcggt cgcgacgtcg ggatggtatt
     2581 tccgcgccag cttgcgaaac gcgctcttga tctcctcctc gctcgccccc ttcgaaacgc
     2641 cgagaatctc gtaatagtcc ttgaatttaa cagccatggt tacgcgtctt ccaaatcccc
     2701 gccccgccgc ggaaagaacc gggccagcag gatgcagatc tcgtaaagca catacatggg
     2761 tccggccatg agcagcaagg tcagcatgtc ctgggtcggg gtgaccgccg cggaaaaaat
     2821 caggatgagg atcaccgcaa acggccgggt gcgccgcagc aggcgcacat ccacgattcc
     2881 cagtttcacc agcaccagca cggcgattgg catttcaaac gccaccccaa acgcgacgat
     2941 gaactgggtg gcgaaggagt aata
//
LOCUS       DATDAY010000097         4540 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_1120947, whole genome shotgun sequence.
ACCESSION   DATDAY010000097 DATDAY010000000
VERSION     DATDAY010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 4540)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4540)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4540
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1120947"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..660)
                     /gene="cyoE"
                     /locus_tag="VIS74_01885"
     CDS             complement(<1..660)
                     /gene="cyoE"
                     /locus_tag="VIS74_01885"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: protein motif:HMM:TIGR01473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HEY5792017.1"
                     /translation="MKAAAANRPEVKFSDLSELFKARLSFLVIITTLVGFLAAWRGPL
                     DYLVFTATLIGTALSAFGASALNQWFERDVDALMRRTRDRPLPARRMSARDGLMLGLL
                     FSIAGVACLGFFVNGLSAALAALTVVSYVWLYTPLKRISTLNTLVGAVPGALPPLIGW
                     AAAADGIQFGGVVLFMILWFWQMPHFMAIAWMYREDYARAGFKMLSVADEGGVVSARQ
                     SV"
     gene            complement(657..1700)
                     /locus_tag="VIS74_01890"
     CDS             complement(657..1700)
                     /locus_tag="VIS74_01890"
                     /inference="COORDINATES: protein motif:HMM:NF014665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0016653 - oxidoreductase activity, acting
                     on NAD(P)H, heme protein as acceptor [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HEY5792018.1"
                     /translation="MPTPTATPYGWTRTGAHLLAILTAFLTFVLIGSGGLVTSHGVGM
                     SVPDWPETYGYNMFLFPISRWVGGIFYEHTHRLLASGVGLLTLILAGWIFFVEPRRWV
                     KVLAAGALVAVVLQGVLGGYRVLLNLNQIGIFHGFLAQSFFISLGILAIVTSRRFVEG
                     RFAREPWSGGLRRMALGVTILIFVQLAIAASMRHAHAGLSIPDFPLAYGQWWPDTGAA
                     ALERINAARVADNEVPTTAGLIWLQMAHRLVACAIFSGVLAFAWLARTRTTDRLGRGL
                     AWLWVGMILGQIGLGAWTVWSNKAADITTLHVALGALSLFVGGLLTFRLFCAPAGEAP
                     SEISTAGLARDPA"
     gene            complement(1738..2328)
                     /locus_tag="VIS74_01895"
     CDS             complement(1738..2328)
                     /locus_tag="VIS74_01895"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEY5792019.1"
                     /translation="MKKTHASTRRENREPGHRLTSVPFGLVLLIVALSLGCLGYFAAN
                     SGGFRADVFGPENRAVAPGTGASLGKRIYVQNCIVCHQASGRGIPRLYPPLAQSEWVL
                     GKSWVGENHLVKIVLNGLHGPTEINNQRYVGAMVPWEKVLNDEEIAAVLSYIRGEWGN
                     DAPPISPEFVAEVRARNLSRNREWTQPELRAIPKND"
     gene            complement(2325..2957)
                     /locus_tag="VIS74_01900"
     CDS             complement(2325..2957)
                     /locus_tag="VIS74_01900"
                     /inference="COORDINATES: protein motif:HMM:NF014486.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit II"
                     /protein_id="HEY5792020.1"
                     /translation="MNRLPRLFLGIFATFAFAWLGLVIYPYLHLGRLQPYEKSDEAFP
                     PPLSEAARAGQRVYAANGCVYCHSQQIRQPYATESDLARGWGSRPTVARDYLREKPTF
                     LGTMRTGPDLSNAGKRLADPVWHYQHLFAPAEMVPGSVMPSFRYLFRVQKIGNAPSPD
                     AVPVSGPHAPPADYEVIPTAEARTLVAYLLSLKHEYPLPEAAPPELTEGR"
     gene            complement(2954..4495)
                     /gene="ccoS"
                     /locus_tag="VIS74_01905"
     CDS             complement(2954..4495)
                     /gene="ccoS"
                     /locus_tag="VIS74_01905"
                     /inference="COORDINATES: protein motif:HMM:NF012343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome oxidase assembly protein
                     CcoS"
                     /protein_id="HEY5792021.1"
                     /translation="MDAGLLLLLAGLLFLSGSAFAAFCWAAREGQFADLQQSAGEILR
                     DPVQERHEVDQSVRLPVLVFLSSAIFWLLMGSLLGLVSAIKMTHPGFLDDAGWLTFGR
                     LRPAHLNAMVYGWASGAGIGVGIWLLARLCHAALRWDGLLLAAAGVWNLGVLVGTAGI
                     LRGDSRGIEWLEFPGYATPFLFVAWGGIAIWAAFLLRHRRRGHLYVSAWYLLAAFLWF
                     PWLYATANLLLIWQPVAGPAQAAVNWWFAHNILGLWFTPLGLAAAFYLIPKVIGEPIH
                     SYYLSLLGFWSLALFYSWNGMHHLIGGPFPAWMVSASVVASLMMFVPVITVAINHHST
                     LRGHFGALRWSPTLRFVVLGSMAYTLVSFQGSLLAIPAVNGVLHFTDFTPAHAHLGMY
                     GFFTMVMFGSVYYLLPRLVGWEWPSAGLIRWHFWLSAGGIALMFVSLTLGGIGQGMIL
                     NDPAIPFLTTLGWVWPFRMARSLSGLLLFAGHLIFALHFGLMLRKYGPERDAPTLLAN
                     PEAAA"
BASE COUNT          983 a         1467 c         1476 g          614 t
ORIGIN      
        1 gaccgactgc cgggcggaaa cgaccccgcc ttcgtccgcc acgctgagca tcttgaatcc
       61 ggcccgcgcg tagtcttcgc gatacatcca ggcgatggcc atgaaatgcg gcatctgcca
      121 gaaccaaaga atcatgaaaa gcaccacgcc gccgaactgg atgccgtccg ccgccgcggc
      181 ccagccgatc aggggcggca gcgcgccggg caccgcgccg acgagggtgt tcagcgtgga
      241 aatgcgcttc agcggcgtgt agagccagac gtagctgacg acggtcagcg ccgccagggc
      301 ggcggacaat ccattcacga aaaatccgag gcaggccacg cccgcgatgg aaaacaggag
      361 gccaagcatc aggccgtcgc gcgcggacat ccgccgggcg ggcagcgggc ggtcccgcgt
      421 gcggcgcatc agcgcgtcca cgtcgcgctc gaaccattgg ttgagggcgg aggcgccgaa
      481 cgcgctgagg gcggttccga tcagggtcgc cgtgaagacg aggtaatcca gcggcccgcg
      541 ccacgcggcg aggaagccca cgagcgtggt gatgatgacc agaaaggaaa gccgcgcctt
      601 gaagagctcg gaaagatcgg aaaatttcac ttcgggacga ttcgcggcgg cggctttcat
      661 gcgggatcgc gggccagtcc cgccgtcgaa atttcggaag gcgcctcgcc cgccggggca
      721 cagaacaggc ggaacgtcag caggccgccc acgaacaagg acaatgcgcc gagcgcgacg
      781 tgcagcgtcg tgatgtcggc ggccttgttc gaccagaccg tccacgcgcc gaggccgatc
      841 tggccgagga tcatgcccac ccaaagccag gcgagccccc gccccaaccg gtccgtggtc
      901 cgcgtgcggg ccagccaggc aaaggcgaga acgcccgaaa aaatcgcgca cgccacgagg
      961 cggtgcgcca tttgcagcca gatcaatccg gcggtcgtag gcacttcgtt gtccgccaca
     1021 cgcgcggcgt tgatccgctc cagcgcggcg gcgcccgtgt cgggccacca ttggccgtaa
     1081 gccagcggga aatcgggaat tgaaagcccg gcgtgcgcgt gccgcatgct ggcggcgatg
     1141 gcgagctgga caaagatcag aatcgtaacg ccgagcgcca tccggcgaag gccgccgctc
     1201 cagggctcgc gggcgaagcg cccctccacg aagcggcgcg aggtgacaat ggcgagaatg
     1261 ccgagcgaga taaaaaacga ctgggcgaga aacccgtgaa aaatcccgat ctgattcagg
     1321 ttcaacagca cgcggtaacc gcccagcacg ccctgcaaca ccaccgccac cagcgcgccg
     1381 gcggcgagca ccttcaccca gcggcgcggc tcgacgaaga aaatccaccc cgccagaatg
     1441 agcgtcagca gcccgacgcc ggaagccagc aggcgatggg tatgctcata aaaaatcccg
     1501 cccacccagc gggagatggg gaagagaaac atattatagc cataggtctc cggccagtcg
     1561 ggcaccgaca tacccacccc atgagaagtc acgagcccgc cgctgccgat gaggacaaac
     1621 gtgaggaacg cggtaaggat ggcgagaagg tgggctcccg tccgggtcca tccatatgga
     1681 gtcgctgtgg gtgtgggcat acggctcctc aaagactgac cgtcctattc atcctgtcta
     1741 gtcgtttttg gggattgccc ggagttccgg ctgggtccat tcccggttcc gggaaagatt
     1801 ccgggcgcga acctcggcca cgaactccgg ggaaatgggc ggcgcgtcat tgccccattc
     1861 gccgcggata tagctcagca cggcggcgat ttcctcgtcg ttgagcactt tttcccaggg
     1921 aaccatcgct ccgacataac gctgattatt gatctccgtc gggccatgaa ggccgttgag
     1981 cacgatcttg acgaggtggt tttcccccac ccagcttttg ccaagcaccc attcgctctg
     2041 tgcgagcggc ggatagaggc gcggaatgcc ccggccggaa gcctgatggc acacgataca
     2101 attttgcacg taaatgcgtt tgcccagcga agcgccggtc ccgggcgcga ccgcgcggtt
     2161 ttccggacca aacacatccg cacggaaacc gccgctgttc gccgcgaaat accccaggca
     2221 gccgagggag agcgcgacaa tcaacagcac cagcccgaac ggaaccgacg tgagccggtg
     2281 gccgggctcg cggttttccc gccgcgtgga ggcgtgcgtt tttttcatcg tccctccgtc
     2341 agctccggcg gcgcggcctc cggcaatggg tattcgtgtt tgagcgaaag caggtaggcc
     2401 accagggtcc gcgcctccgc ggtggggatg acttcatagt ccgccggcgg cgcgtgcggg
     2461 ccgctcaccg gcaccgcgtc cggcgagggc gcgttcccga ttttttgcac gcgaaaaagg
     2521 taacggaagg agggcatgac cgagcccggc accatttccg cgggagcgaa gagatgctga
     2581 taatgccaga ccgggtcggc cagccgcttg ccggcattgc tgagatcggg accggttcgc
     2641 atggtgccga gaaaggtcgg tttttcgcgc agataatcgc gcgccaccgt gggtcgcgag
     2701 ccccagccgc gcgcaaggtc cgattcggtc gcgtagggct ggcggatctg ctgggaatgg
     2761 caatacacgc agccattcgc cgcataaacg cgctggccgg cccgggccgc ctccgacagc
     2821 ggcggcggaa aggcttcatc cgatttttcg tagggctgta ggcggccaag gtggaggtag
     2881 ggatagatca ccaaccccag ccaggcgaag gcaaacgtgg cgaagatgcc gaggaacaag
     2941 cggggcagcc ggttcatgcg gcggcctccg gattggccag gagcgtgggc gcgtcccgct
     3001 cggggccgta tttgcggagc atgagcccaa aatgcagcgc gaagatcaga tgccccgcga
     3061 acagcagcag gcccgaaagg gatcgcgcca tccgaaacgg ccagacccac cccagcgtgg
     3121 ttaaaaaggg aatcgccgga tcgttgagga tcatcccctg gccaatgccg cccagggtca
     3181 gcgagacaaa catgagcgcg atgccgccgg ccgacagcca gaaatgccag cggatcaggc
     3241 cggccgaggg ccattcccac cccacgaggc ggggcaggag gtaatacacc gagccgaaca
     3301 tgaccatcgt gaaaaatcca tacatgccca gatgcgcgtg cgcgggcgtg aagtcggtga
     3361 aatggagcac gccattgacc gcggggatcg ccagcaggct gccctgaaaa ctcaccagcg
     3421 tgtaggccat ggaacccagc acgacgaacc gcagcgtggg gctccagcgc agggcgccga
     3481 agtgcccgcg cagggtggag tgatggttga tcgccacggt gatgaccggc acaaacatca
     3541 tgaggctcgc caccacgctc gcgctgacca tccacgccgg aaagggcccg ccgatgaggt
     3601 gatgcatgcc attccagcta taaaaaaggg ccagcgacca aaacccgaga aggctcaggt
     3661 aataactgtg gatcggctcg ccgatgacct tgggaatcaa ataaaacgcc gccgcgagcc
     3721 cgaggggcgt gaaccagagg ccaaggatgt tgtgcgcgaa ccaccagttc accgccgcct
     3781 gcgccgggcc ggcgaccggc tgccagatca ggagcaaatt cgccgtggcg taaagccagg
     3841 gaaaccacag gaacgccgcg agcagatacc aggccgaaac gtaaagatgc ccccgccggc
     3901 ggtggcgcag caggaacgcc gcccagatgg cgatgccgcc ccacgcgacg aagagaaacg
     3961 gggtcgcgta accggggaat tccagccatt cgatgccgcg gctgtcgccg cgcaggatgc
     4021 cggcggtgcc caccaggacg ccgagattcc aaacgcccgc ggcggccagc agcaacccat
     4081 cccaccgcag cgccgcgtgg cagagccgcg ccagcagcca gatgccgacg ccgatgcccg
     4141 cgccggaggc ccagccgtaa accatcgcat tcaaatgcgc cggccggagg cggccaaacg
     4201 tcagccaccc cgcgtcatca agaaagccgg ggtgggtcat tttaatggcg gaaaccaacc
     4261 cgagcaatga tcccatcaac agccagaaaa tcgccgaact cagaaatacg agcacgggga
     4321 gccggacgga ttgatccacc tcgtggcgtt cctgaaccgg atcgcggagg atttccccgg
     4381 cggactgctg cagatcggca aattgcccct cccgcgccgc ccagcaaaag gccgcgaagg
     4441 ccgagccgct taaaaaaagc agcccggcaa ggagaagcag caggcccgca tccacctacg
     4501 acgcttcgct gaccttttcc tcccgaaccg gcaagggctt
//
LOCUS       DATDAY010000098         2052 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_1896990, whole genome shotgun sequence.
ACCESSION   DATDAY010000098 DATDAY010000000
VERSION     DATDAY010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2052
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1896990"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..389)
                     /locus_tag="VIS74_01910"
     CDS             complement(<1..389)
                     /locus_tag="VIS74_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEY5792022.1"
                     /translation="MAHLQDLIAASVKNYKEGSIVKGHILEIRNREFLVDIGYKSEGV
                     IPASEFEEPGDVEIGDEIEVLLERLENDEGMVVLSKEKAAQRQNWEKIVKVFQGEGLI
                     RGKVRSVVKGGLTVNIGVEAFLPASQI"
     gene            551..1498
                     /locus_tag="VIS74_01915"
     CDS             551..1498
                     /locus_tag="VIS74_01915"
                     /inference="COORDINATES: protein motif:HMM:NF015509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HEY5792023.1"
                     /translation="MGFFDLLQISAPVFLVLIIGFAVRRMKLLTEEADHTLLRLTINL
                     FYPSLLLDLIIGNEALLRWDYLLLPPVFGFVTVAAGFGASWLGAKWAGIPAGPPRRTF
                     SFTCGLPNYGYLALPICFMLFGRETSAVLLGYNLGVEIAYWGLGLMVLTGEWGMRGLR
                     KIWNVPVLSLLVALGLNLAGAGRWMPEWIDRTYTMLGYCAVPVALLLTGALFADYAQP
                     AHFLESKRTFGVSLLVRLGVMPVFFLLLAWLLPLETHLRQVLVVQGAMPAAVAPLAVA
                     RFYGGDVPTGFKVILGTTLAGFVTVPLWLFWGIHFLRLH"
     gene            1646..>2052
                     /locus_tag="VIS74_01920"
     CDS             1646..>2052
                     /locus_tag="VIS74_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEY5792024.1"
                     /translation="MQPLGSITLAEFSGFLAAKSQPAYRAKQVFEWVYRKREFGIDRM
                     SNLPADLRSTLAAEFDLSLPEVVRVQGSEDTTRKFLFRFPDGDLVESVLIPASPALYG
                     EKSDRRTICLSSQVGCAYGCKFCASGLDGWKRNL"
BASE COUNT          325 a          592 c          650 g          485 t
ORIGIN      
        1 tcgatctggg aggcgggaag gaaggcttcg acaccgatgt tgacggtgag gccgcctttg
       61 acgaccgagc ggactttgcc gcggatgagg ccttcgccct ggaaaacctt gacgattttt
      121 tcccagttct gacgctgggc ggccttctcc ttggagagaa cgaccatgcc ttcgtcgttt
      181 tcgagacgtt cgaggaggac ttcaatttcg tcgccgattt cgacgtctcc gggttcttcg
      241 aattccgaag cgggaatcac gccttcggat ttgtagccaa tgtcaacgag gaattcgcgg
      301 ttgcggattt cgaggatgtg tcctttgaca atgcttcctt ctttatagtt cttgaccgac
      361 gctgcgatca aatcttgcaa atgagccatg ggttgaatgt ttaggttaat aaaaagccac
      421 acgcgtcgag actttcggcg cgagccggat ccggctagtg gcggaacggt atttctatcc
      481 catcaccacc aaagctcaag cattattctt gcaaataggc attttcgccg tttgttgaag
      541 ggccgcccac atgggatttt tcgatctgct gcaaatttcc gcgccggttt ttctggtgct
      601 catcattggc ttcgccgtcc ggcggatgaa gctcctgacc gaggaggccg accatacgct
      661 gttgcggttg accatcaacc tcttttatcc ctcgctgctg ctcgatctga tcatcggcaa
      721 cgaggcgctg ctgcggtggg attaccttct cctgccgccg gtgtttggct ttgtgacggt
      781 ggccgcgggt ttcggcgcga gctggctggg ggcgaaatgg gcgggcattc ccgccgggcc
      841 gccgcgccgg acgttcagtt tcacctgcgg attgccgaat tacggctacc tcgcgctgcc
      901 gatctgtttc atgctctttg gccgcgaaac ctcggcggtg ctgctgggat acaatctcgg
      961 agtcgaaatc gcctattggg ggctgggcct catggtgctg accggcgaat ggggaatgcg
     1021 gggcttgcgc aagatttgga atgttccggt gctcagcctg ctggtggcgc tggggttgaa
     1081 tctggccggg gcggggcggt ggatgccgga gtggattgac cggacctaca cgatgctggg
     1141 ctattgcgcg gtgccggtcg cgctgctctt gaccggcgcg cttttcgccg actacgcgca
     1201 gccggcgcat tttctggaaa gcaaacggac gttcggcgtc agcctgctgg tgcggctggg
     1261 cgtcatgccg gtgttcttcc tgctgctggc ctggctgttg ccgctggaaa cccacctgcg
     1321 acaggtgctc gtggtgcagg gcgccatgcc cgcggcggtc gccccgctgg cggtggcgcg
     1381 attctacggc ggcgacgtgc cgacgggctt caaggtcatc ctcggcacga cgctggcggg
     1441 atttgtcacg gtccccctgt ggcttttttg gggcatccac tttctccgtc tgcattgact
     1501 cacgcggaga cacgggggcg cggaggaaaa acttggaact ttatccccaa ccctttcctc
     1561 ctgtctttct ccgcgtcttc gcgcctccgc gtgagattcc atccggggca ttgaattcag
     1621 gccggaatct ggcagagtag ggggcgtgca accactcggc tccattacat tggcggaatt
     1681 cagcggcttc ctcgcggcga agtcgcagcc ggcgtaccgg gccaagcagg tgttcgagtg
     1741 ggtgtatcgc aagcgggagt tcgggatcga tcgcatgtcg aacctgcccg ccgacctgcg
     1801 ctccacgctg gcggcggaat tcgatctctc gctgcccgag gtggtccgcg tgcagggttc
     1861 cgaggacacc acgcgcaaat ttttgttccg ctttccggac ggcgaccttg tcgagtccgt
     1921 cctgattccg gcatccccgg cgctttacgg cgagaaatcc gaccggcgga ccatctgcct
     1981 ttcctcgcag gtcggctgcg cctacggctg caagttctgc gcgagcggat tggacggatg
     2041 gaaacgcaac ct
//
LOCUS       DATDAY010000099         2483 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_1467466, whole genome shotgun sequence.
ACCESSION   DATDAY010000099 DATDAY010000000
VERSION     DATDAY010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2483)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2483)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2483
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1467466"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..615)
                     /locus_tag="VIS74_01925"
     CDS             complement(<1..615)
                     /locus_tag="VIS74_01925"
                     /inference="COORDINATES: protein motif:HMM:NF018437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3D domain-containing protein"
                     /protein_id="HEY5792025.1"
                     /translation="MTNPLTLASAAFFAFAAAAFASKDAGRKSVPEVCQRFENIRTTA
                     YSTGPQHNGRYGARNAIDGPLKSGKVSSAAADWSRWPLGTRFRVAETGGEYIVDDIGI
                     AMAGTGTIDLFKPSEAGVDRWGVRHVTIEVLEWGSLEKSLRILEERTRFAYIRKMVRD
                     LKALDETTAIVVLTGYGSIATAIESLRLGAIHYLSKPVDVDQILA"
     gene            832..2334
                     /gene="mqo"
                     /locus_tag="VIS74_01930"
     CDS             832..2334
                     /gene="mqo"
                     /locus_tag="VIS74_01930"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: protein motif:HMM:NF009875.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008924 - malate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HEY5792026.1"
                     /translation="MKYSKHLENPDVILIGSGVMSANLAALLKCLEPRLRIQVYEVTD
                     ELAQESSHGWNNAGTGHAGICELSYTPHREAGGTVNVKKAIEIFEQFEKSRQFWSYAV
                     GSGMIDRPKDFINPVPHLSFVHGPEQVDYLRARFAGLTAHHFFQGMEYSTDRATIARW
                     APLLLEGRGEGAIAATKMNGGTDVNFGEISRKLLNWLYLQDGGGIASRHRVIGLERVG
                     PDWEARIRDLESGEIRRNRAPFVFIGAGGGSLPLLQKSGIPESKGLGGFPIGGQWLVC
                     ENPEIVQKHQAKVYGQALGAAPTMAVPHLDTRVLDGKKTLLFGPFAAWTTKFLHREGS
                     LLDLPLSVKPDNLLTLLKIGLSNLDLIKYLVQQGTQSMADRLEVLHVFYPAARAADWK
                     LIDAGIRVQAIKKTDGEAGIVHYGTEVVTNADRSISALLGASPGASVSVHIVLEVIQK
                     CFGHLLKTPEGRSRMRAMIPAWDQDLKLAENAERYRALSRRADKILALLS"
BASE COUNT          495 a          796 c          744 g          448 t
ORIGIN      
        1 cgcaagaatc tgatcgacgt cgaccggctt gctcagatag tggatggcgc cgagccgcag
       61 gctctcgatg gccgtggcga tgctgccata gccagtgagc acgacgatcg ccgtcgtctc
      121 gtcgagcgcc ttgagatcgc ggaccatctt gcggatgtag gcaaagcggg tgcgctcctc
      181 cagaatccgc aggctcttct cgagcgagcc ccactccagc acttcgatcg tcacatggcg
      241 caccccccag cggtccactc cggcttcact gggcttgaaa agatcaatgg tgccggtgcc
      301 ggccatggcg atgccaatgt catcgacgat gtattcgccg cccgtctcgg ccacgcggaa
      361 acgcgtgccc agcggccagc gggaccagtc cgcggcggcg ctgctgacct tgccggattt
      421 gagcgggccg tcgatggcgt tgcgggcgcc gtagcggcca ttgtgctgcg ggccggtgct
      481 gtaggcggtg gtccggatat tttcaaaacg ctggcagacc tccggaaccg atttccggcc
      541 agcatccttc gaagcgaatg ccgcggccgc aaacgcaaag aacgcggcgg aggcaagggt
      601 gagaggattc gtcatagagc cgggaaaagt agggagcatc cccccgagcg caacccctcc
      661 acggcatttt atttgcaaat cattggctat aagcggccga aaaatgaaaa aaattatcag
      721 tcactgaaat tgcaccctta cttccgtcct tacttccgcc gtttggcgtg caggatggag
      781 ctttacattg caccccgcag gttggttgat agctcccatt cccgcgaccg catgaagtat
      841 tccaagcacc tcgaaaatcc cgacgtcatt ctcatcggaa gcggcgtcat gtccgccaac
      901 cttgccgctt tgctcaaatg tctggagccc cggctgcgca tccaggttta tgaggtcacc
      961 gacgaactcg cgcaggaaag ctcccacggc tggaacaatg ccggcacggg tcatgcgggc
     1021 atctgcgagc tgagctatac cccgcaccgc gaggccggcg gcacggtgaa cgtgaagaag
     1081 gcgatcgaga ttttcgagca attcgaaaag tcccggcagt tctggagcta cgcggtgggc
     1141 agcgggatga tcgaccggcc gaaggatttc atcaaccccg tgccgcatct cagcttcgtg
     1201 cacgggccgg agcaggtgga ttacctgcgg gcgcgcttcg cgggattgac cgcgcaccat
     1261 ttcttccagg gaatggaata ctccacggac cgcgcgacca tcgcccgctg ggcgccgctg
     1321 ctgctggaag gccgcgggga gggggcgatc gccgccacga aaatgaacgg cggcaccgat
     1381 gtgaacttcg gcgagatttc ccgaaagctg ctgaactggc tctatctaca ggacggcggc
     1441 ggaatcgcca gccgccatcg cgtcatcggc ctggaacgcg tgggcccgga ctgggaggcg
     1501 cgcatccggg atctggaatc cggcgaaatc cgccggaacc gcgcgccctt cgtcttcatc
     1561 ggcgccgggg gcggcagcct gccgctgctg cagaaatccg gcattccgga aagcaagggc
     1621 ctgggcggat tccccatcgg cggccagtgg ctggtctgcg aaaatccaga aatcgtccaa
     1681 aaacaccagg cgaaggttta tgggcaggcg ctcggcgccg ctccgaccat ggccgtgccg
     1741 cacctcgaca cacgcgtgct ggatggcaaa aagacgctcc tcttcggccc cttcgccgcg
     1801 tggaccacga agtttctcca ccgggagggc agcctcctcg atctcccgct ttcggtgaag
     1861 ccggacaatc tgctgacgct gctcaagatc gggctcagca atctggatct catcaaatac
     1921 ctcgtccagc aaggcaccca aagcatggcg gatcgcctgg aagtgctcca tgtcttttac
     1981 cccgcggcca gggcggcgga ttggaaattg atcgatgccg gcatccgggt tcaggccatc
     2041 aaaaaaaccg acggcgaagc gggcatcgtc cattatggca ccgaggtcgt cacgaatgcc
     2101 gaccgttcca tttccgcgct gctgggcgcc tcgcccggcg cctccgtctc cgtccacatc
     2161 gtgctcgaag tcatccagaa atgttttggc catctattga aaactccgga aggccggagc
     2221 cgcatgcggg ccatgattcc ggcctgggac caggatttga agctggccga aaacgcggaa
     2281 cgctaccgcg cactcagccg ccgggccgac aaaattttag cgcttttatc ctgacatgcc
     2341 gcaaaaccgg ggtaatccat gaaacccctc cccacctttc tcgcgggggc gctggtgatt
     2401 gccctcgccg gctgctcgct gttcaaaccc aagcccgccc cgcccgtccc tcccgaacag
     2461 cagatcgtca accagatgat tcc
//
LOCUS       DATDAY010000100         2360 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_72123, whole genome shotgun sequence.
ACCESSION   DATDAY010000100 DATDAY010000000
VERSION     DATDAY010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2360)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2360)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2360
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_72123"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..991)
                     /locus_tag="VIS74_01935"
     CDS             complement(<1..991)
                     /locus_tag="VIS74_01935"
                     /inference="COORDINATES: protein motif:HMM:NF037004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEY5792027.1"
                     /translation="MVLPLVLGAFVMGRAAAEEDAAEKRQVVTAVSRLLEQGHYTREK
                     LNDEMSAKFLDSYLRLLDYNRLYFLQSDIDEFKRKYGTTLDDSLLRGDLEPASAIYNR
                     FKQRLEARIKSNLERIPAKQDYTSSRTVQINRQKAEWPKDQADADQLWADRLEAELLQ
                     EELSKSREDSPAKILTRRYNQILRNIHEQDEEDIIKTYLSALAQTYDPHSDYMSPSDM
                     ENFSISMRLSLVGVGAVLRSDEGYARVVEVVPGGPADRDGRLRVNDRIAAVAQGEKDF
                     EDVMDMKLDKVVEKIRGEKGTTVRLLVVPSDAADPSKRQVIDIVRDEVKLKEQE"
     gene            1058..1702
                     /locus_tag="VIS74_01940"
     CDS             1058..1702
                     /locus_tag="VIS74_01940"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY5792028.1"
                     /translation="MNSDSRSDREGALKFKTFTGGIFDTNGYLLQTPAGNVLVDAPDG
                     AAEAFAGESISHLLLTHGHYDHVVDAARIQREHRCPVYFHPDTTALVSTAGAFRKFGF
                     ALDIEPFRADHLITEGPAQRFGELEMDVFEVPGHCPGSLCFYHRPSGLLFGGDVLFAG
                     AIGRWDLPGGNLQLLLSGIRQKLFPLPENTRVLSGHGPETTLGREKRANPFFQR"
     gene            1776..>2360
                     /locus_tag="VIS74_01945"
     CDS             1776..>2360
                     /locus_tag="VIS74_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEY5792029.1"
                     /translation="MPRYTYVALDSRGQETTGVIEAGTQNEAIGSLRQSGYFPKSIAE
                     ESKAKLARNAKTAKAPAAKGLSKDISLNIPFLQRKTIKGKALMIFTRQLATLIDAGLP
                     LLRGLTVLSKQEPDPVLRRTILNLAESVQSGGTFSESLGGHPKIFNKLYVNMVKAGEL
                     GGVLEIVLIRLAEFQEKAQKIKNKVMSAMFYPIIV"
BASE COUNT          469 a          742 c          674 g          475 t
ORIGIN      
        1 cctcctgttc cttgagcttc acctcgtcgc gaacgatgtc gatgacctgg cgtttggacg
       61 gatccgccgc gtccgacggc accaccagca ggcggaccgt ggtgcccttt tcgccgcgga
      121 ttttttccac gaccttgtcg agcttcatgt ccatgacgtc ctcgaaatcc ttctcgccct
      181 gggccacggc ggcgatgcga tcattcacgc gcaggcggcc gtcgcgatcc gccgggccgc
      241 cggggaccac ctcgaccacg cgggcatacc cctcatccga acgcagcacg gcgccgacgc
      301 ccacgaggga aagccgcatc gaaatggaaa agttctccat gtcggacggg ctcatgtagt
      361 ccgaatgcgg gtcgtatgtc tgggccagcg cggagaggta ggttttgatg atgtcctcct
      421 cgtcctgctc gtggatgttg cggaggattt gattgtaacg gcgcgtcagg atcttggccg
      481 gcgagtcctc gcggcttttg ctgagttcct cctgcagcag ctcggcttcg aggcgatccg
      541 cccagagctg gtcggcatcg gcctgatcct tcggccattc cgccttctgg cggttgattt
      601 gcacggtccg gctggaggtg tagtcctgct tggcggggat gcgttcgagg ttgctcttga
      661 tgcgggcttc gaggcgctgc ttgaagcggt tgtagatggc cgaggccggc tccaaatcgc
      721 cgcgcagcag ggagtcatcg agcgtggttc cgtatttccg cttaaattcg tcaatgtcgc
      781 tctggagaaa ataaagccgg ttgtaatcca gcaggcggag ataactgtcg agaaacttgg
      841 cggacatttc gtcgttcaat ttctcgcggg tgtaatggcc ttgctccagc aaccgggaaa
      901 ccgccgtgac gacctgccgc ttttccgcgg catcctcctc ggccgcggcc cgccccatta
      961 caaaagcgcc gagcaccagc ggcagaacca ttgactgacg aataggaaaa cgcattataa
     1021 aagacatact aggttactcg gaagcgggac aaatgcaatg aattcagatt caagatcaga
     1081 ccgcgaaggc gcgctaaagt tcaaaacttt taccggaggc atctttgaca ccaacggtta
     1141 cctcctccaa acgcccgccg gaaacgtgct ggtggatgcg ccggacggag cggcggaagc
     1201 ttttgccggc gaatccattt cccacctgct gctcacccac ggccattacg atcatgtcgt
     1261 ggacgcggcg cgcatccagc gcgagcaccg ctgcccggtt tattttcatc ccgacaccac
     1321 ggcgctggtg agcacggcgg gcgccttccg caaattcggc ttcgctttgg acatcgagcc
     1381 cttccgggcc gatcacctga tcaccgaagg ccccgcccaa cgcttcggcg aactggaaat
     1441 ggatgtcttc gaggtgccgg ggcattgccc gggcagcctg tgtttttacc accgtcccag
     1501 cggcctgctt ttcggcggcg acgttctttt cgccggcgcg atcggccgat gggatttgcc
     1561 cggcggcaac ctgcaactcc tgctgagcgg aatccgccaa aagctgtttc cgctgccgga
     1621 aaacacccgc gtgctttccg gccatgggcc ggaaacgacc cttggccgcg aaaagcgggc
     1681 gaatcccttt ttccagcgct aaacgccggc ttctacgctg gacaaacgcg ccgggcgcgg
     1741 tttattgaac tgagtcaccc tctttacata tatatatgcc gcgttatacc tacgttgcgc
     1801 tcgactcgcg gggccaggaa accactggcg tcattgaagc cggcacccag aatgaagcaa
     1861 tcggctccct ccgccagtcc ggctattttc cgaagagcat cgcggaggaa tccaaggcca
     1921 agctggcaag aaacgcgaaa accgccaagg ccccggcggc aaagggatta agcaaggata
     1981 tcagcctcaa tatccccttc ctccagcgca agacaatcaa gggcaaagcc ctcatgattt
     2041 ttacgcgcca gctggcgacg ctcatcgacg ccggcctccc gctcctgcgc ggcctcacgg
     2101 ttctctccaa acaggagccc gatcccgtcc tgcgccggac catcctcaat ctcgcggaat
     2161 ccgtgcaaag cggcggcacc ttctccgaaa gcctgggcgg gcaccccaag atcttcaaca
     2221 aactttacgt gaacatggtg aaggccggcg aactcggcgg cgtgcttgaa atcgtgctca
     2281 tccgcctggc ggagttccag gaaaaagccc aaaagatcaa aaacaaggtc atgtccgcga
     2341 tgttctatcc gatcatcgtg
//
LOCUS       DATDAY010000101         6115 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_376529, whole genome shotgun sequence.
ACCESSION   DATDAY010000101 DATDAY010000000
VERSION     DATDAY010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 6115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6115)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6115
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_376529"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..214
                     /locus_tag="VIS74_01950"
     CDS             <1..214
                     /locus_tag="VIS74_01950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792030.1"
                     /translation="VFSRLEVIELAAADYRNALVLSAGRGLTSGVIYDALHVLCAMKS
                     HCERIYTYNVSHFRRLAPEALTICLP"
     gene            381..743
                     /locus_tag="VIS74_01955"
     CDS             381..743
                     /locus_tag="VIS74_01955"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="clan AA aspartic protease"
                     /protein_id="HEY5792031.1"
                     /translation="MITLKNPRIPELQPVEVEALADTGSVHLCIPEHVRLQLRLEERD
                     RKEVTLADGSKRLIPYVGPIEVCFKNRIGFGGAIVRGDQVLLGAIPMEDMDLVVHPKT
                     RSIDVNPENPNFACSIAK"
     gene            1079..4399
                     /locus_tag="VIS74_01960"
     CDS             1079..4399
                     /locus_tag="VIS74_01960"
                     /inference="COORDINATES: protein motif:HMM:NF018190.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HEY5792032.1"
                     /translation="MIPLKFPLFLLFSTGLFLPLAQGGDAAIPNAAFEAAGSPVAGWK
                     ITGDGSLDEKEKVEGARALVLRRDPVNLPATSAISESFPITPGVWELSGAYANDLYSP
                     DVSFNVSVGIQTFDESGKALKQRRLLAVSNKNAWTHFKERLDIPAGAASAVVKVEFNK
                     THGDFRLDALALKYIGASIPMEGGDRKVIFQTNRVGNLFYPGDSARLEMTLETPAQLS
                     GDALRVDWQVTDFYAAPASAPGQSPLVSAGKTARGWNLYRAVLDLSTLPLKVGPYYEL
                     RTSVNLGASSPARDTASFAILPEAASKQLDPLASPFGAHTWNATVYEYFPLAARLGIR
                     RALVFWGWPEKPPYTPLFAKDKGDGYLRRIGWPKRFGIAPYGVLYPIMNIEHREGVDY
                     SDEALREGIRQSIEKFKGDGLWGFQIGNEPPAFNPEMVKRDVEAYKVAYEAIKKTDPN
                     FVVIGTAVGASEEFFKAGFQAYQDAYNVHAYSDLGELRLAMKKYQQLFDKYGGKKPIW
                     STEIGSKSQGLPRDVIARDIIRKAVCFFADGGGFFTWFAVGGMPDPEGERAGSYSDSM
                     DLFGSKFGMHLPRLDAVAFYHLINILGAKTFVTELEYPDGLHGFLFRDKQANSLFVFW
                     KDAGGKDVFVQMPGIHEVALTWMNGATRQLEANGQGINLRIGEDPVFLAFRSDETELP
                     KELGAPGSVKIEALPEVLTQGETAEVKVRVGDRQSLQLSGPEPWKIEAAVETPNPDGS
                     RLLTYRIPVPGDTTARAATFTLSDETVTTAGDTELRFSIPVKSKIEVDVLPVAGAKEG
                     DAAIRVRLSNHSDTAQPVNWKAEILNELPMAGGTYRVTDAKPSSAFFQGVAADKLTLK
                     PRETWEIILPMGGTDRQTIYKIRAEATDSTGNVVPRERSVGGFARAVRVSGKPAAEAW
                     AKAPVYLLNEARQFCIPRGTSRPWEGEKDLSGTMQFLWDDQNLYVRSEVVDNVFANPK
                     DDESLWNQDGLQFLFDPYRGELQSRGRYDYSMGLGQKGLQAWRHMSASPGIKAGPAPE
                     IQLTVTPASKDNGNRTYEVTIPWAQLAPFQPKVGGDLGTTMIINEDDGEGRKSTIGWY
                     GGVHLKEAAFVGDLILAE"
     gene            4451..>6115
                     /locus_tag="VIS74_01965"
     CDS             4451..>6115
                     /locus_tag="VIS74_01965"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF014156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HEY5792033.1"
                     /translation="MTAAVSAIHFDSESRSFVLRLKTSAYLLRILPEGAVRHVGFVPL
                     PPEAPATLAAAHLTEYKDANFVWDWQGTPFEYPAFGDVSYREAAFKVAFTQALAELTA
                     EEAPHVPVRDVRLRYERHEIHPDAEPLGAPAHGHPPQVATPRETLCLRLKDELGDFYV
                     TLFYRLTPEQDLIERWVEVENRTNAPVEIETVDFGVLNLPCGHYELTRAAGAWAREFA
                     PVTQPIEQGVFVLDQRGLNTGHAVNPFFHVCERGQATEEAGTVYFGAIAWSGNWRLRF
                     ESLPGGNCRIFAGYEASDFALTLGPGESHKTPAFLFGCSSEGRGGASRRLHRFLRERV
                     LPGHGPEEFRPVLYNSWEATYFDLSLENQTELARVAADIGVELFVVDDGWFGARRHDR
                     AGLGDWVVSQDVFPGGLQPLADEVRRLGMKFGLWVEPEMVNPDSDLYRAHPDWVLHFP
                     GRPRTTSRTQMILDFGRPEVVQYLLDALDALVRENGVDFFKWDMNRYVSEPGSVAGKA
                     IWRKHVEGLYRIWDALRRAHPHLDIQSCSGGGGRIDPGILARADQVW"
BASE COUNT         1149 a         1923 c         1897 g         1146 t
ORIGIN      
        1 tgtcttctca cgactggaag tcattgagct ggccgccgcc gattatcgca acgctctggt
       61 tctttcggcg gggagaggat tgacgagcgg ggtcatttac gacgcacttc acgtcctctg
      121 tgcgatgaag tcgcattgtg agcgaatcta cacctacaac gtctcccatt tccgccgcct
      181 cgctccggaa gccttgacga tctgccttcc gtgattgaac gccgagccgg ccctgccctt
      241 gatttttcat ccgcatgcat ctcattcatc cgtggttgaa aagatttgaa cccgcgggaa
      301 atccgggagg aaacgccata aaaggaattg gcacccctgg cctcgccgga tacactttca
      361 aggcggggct cattctgacc atgatcactc tcaagaatcc acgaattccg gaattgcaac
      421 cggtggaagt ggaagcgctg gcggatacgg gatccgttca cctttgcatc cccgaacacg
      481 tccggctcca acttcgcctg gaggagcgag accggaagga agtcaccctt gcggatggga
      541 gcaagcgcct gattccgtat gtcggtccca ttgaagtttg cttcaagaat cgcatcgggt
      601 ttgggggagc cattgtgagg ggggatcagg tgttgcttgg cgcaatcccc atggaagaca
      661 tggacttggt ggttcatccg aagactcgat cgatcgatgt taatccagag aatcccaatt
      721 ttgcctgctc gatcgcgaaa taaggcccca accttaggaa aatcgctgat tcaatcggag
      781 gaagggaaaa tatccgccga tttcaccgat ttttgccgat tttttgcggc gaggatcgcg
      841 gatttttaaa aatctgcgtc catctgcgcc gcaacgcacg cgaagcggcg gccaggcttt
      901 caaatcggtg gactcctatc cggtttcttt ttcaaaccgt cgatgaaaga tcaaaggctg
      961 tgaaatcgag tttatccgcg tggatcctgt tcatccgtga ttgaagcttg agtcgttcag
     1021 cgcgtttttt caccttgtcc gattgttata tcaatatatc gtttaaaact tccgtcccat
     1081 gataccgctg aaattccccc ttttcctcct gttttccacc ggtttatttc ttcccctcgc
     1141 ccagggcggg gatgccgcca tcccgaacgc cgcgtttgag gcagccggat ccccggtggc
     1201 gggctggaag atcacgggcg acggaagcct ggacgaaaag gagaaggtcg aaggcgcgcg
     1261 ggcgcttgtc ttgcggcggg atccggtgaa tctgccggcg acgagcgcaa tcagcgagtc
     1321 tttcccgatc acgccggggg tgtgggaact ctccggggcg tatgccaatg acctctacag
     1381 cccggacgtc tccttcaacg tctcggtggg catccaaacc ttcgacgaat ccggcaaggc
     1441 cctgaagcag cgccggctgc tggcggtgtc gaacaaaaac gcgtggacgc atttcaagga
     1501 acgcctggac atccccgccg gcgcggccag cgcggtggtg aaggtcgagt tcaacaagac
     1561 gcacggcgac ttccggctcg acgcgctcgc gctcaaatac atcggcgcct cgattccgat
     1621 ggaaggcggc gaccgcaagg tgatcttcca gacgaaccgg gtcggaaacc tgttttatcc
     1681 gggtgattcg gcgcggcttg aaatgacgct ggaaacgcca gcgcagcttt ccggtgacgc
     1741 gctccgggtg gactggcagg tgacggattt ttacgcggcg ccggcgtccg cgccggggca
     1801 aagccccctg gtctccgcgg gaaaaaccgc ccggggctgg aatctctacc gcgcggtgct
     1861 ggatctttcg acgctgccgc tgaaagtcgg gccgtattac gaactgcgca cgtcggtgaa
     1921 cctcggcgcc tcgtccccgg cgcgcgatac cgcgtcgttt gcgatcctgc cggaggcggc
     1981 ctccaaacaa ctcgatcccc tggcctcccc ctttggcgcg catacgtgga atgcgacggt
     2041 ttatgagtat ttcccgctcg cggcccggct gggcatccgg cgcgccctgg tgttctgggg
     2101 ctggccggag aagccgccct acaccccgct gttcgcgaag gacaagggcg acggttattt
     2161 gcgccgcatc ggctggccca agcgattcgg gatcgccccc tacggcgtgc tttacccgat
     2221 catgaacatc gagcaccgcg agggagtgga ttattcggac gaggccctgc gggagggaat
     2281 tcgccagagc atcgagaaat tcaagggcga cggcctctgg ggattccaga tcggcaacga
     2341 gccgcccgcc ttcaaccccg aaatggtcaa acgcgacgtg gaggcctaca aggtcgccta
     2401 cgaggcaatc aaaaaaaccg acccgaattt tgtcgtcatc ggcaccgccg tcggcgcgag
     2461 cgaggagttt ttcaaggcgg gattccaggc gtatcaggac gcctacaacg tccacgcgta
     2521 ttcggacctg ggcgaactgc gcctggcgat gaaaaaatac cagcagcttt ttgacaaata
     2581 cggcggcaaa aagccgatct ggtccaccga gatcggctcc aagagccagg ggctgccccg
     2641 cgacgtgatc gcgcgggaca tcatccgcaa ggcggtttgc ttcttcgcgg acggcggcgg
     2701 gttctttacc tggttcgccg tgggcggcat gcccgacccc gagggggagc gggcgggcag
     2761 ttattccgac tcgatggatc tcttcggctc gaaattcggc atgcatctgc cccggctgga
     2821 cgcggtcgcc ttttaccatc tcatcaacat cctcggggcc aagacgttcg tcacggaatt
     2881 ggaatacccg gacggcctgc atggcttcct tttccgggac aagcaggcga actcgctctt
     2941 cgtgttctgg aaggacgcgg gcggcaagga tgtcttcgtg cagatgcccg gcattcacga
     3001 agtggcgctg acgtggatga acggcgccac gcgccagctc gaagcgaatg ggcagggcat
     3061 caatctgcgc atcggcgagg atccggtatt cctggcgttc cgcagcgatg aaaccgagct
     3121 gccgaaggaa ttgggcgcgc cggggtccgt gaaaatcgaa gcgctgcccg aggttctcac
     3181 gcagggcgaa accgccgagg tcaaagtgcg ggtcggcgac cgccagtccc tgcagctctc
     3241 cggtccggag ccatggaaaa tcgaggcggc ggttgaaacg ccaaacccgg atggctcgcg
     3301 gctgctcacg tatcggattc cggtgccggg cgacaccacg gcccgcgccg ccaccttcac
     3361 gctctcggac gaaaccgtga ccaccgcggg cgacaccgag ctgcgcttct ccattccggt
     3421 gaaaagcaag atcgaggtgg atgtcctgcc cgtggccggc gcgaaggaag gcgacgccgc
     3481 gatccgggtt cggctctcca accattcgga caccgctcag cccgtgaatt ggaaggcgga
     3541 aattctcaac gagctgccca tggccggcgg cacctaccgg gtgaccgacg cgaagccgtc
     3601 cagcgccttc tttcaggggg tggccgcgga caagctgacg ctgaagccgc gcgagacatg
     3661 ggaaatcatc ctgccaatgg ggggaaccga ccgccagacc atttacaaaa tccgggcgga
     3721 agcgaccgac tccaccggca acgtggtgcc gcgggaacgc agcgtcggcg gattcgcccg
     3781 cgccgtgcgg gtcagcggca agcccgccgc ggaagcctgg gcgaaggctc ccgtctatct
     3841 gctcaacgaa gcccggcaat tttgcattcc gcggggaacc tcaaggccct gggagggtga
     3901 aaaggatctc tcgggaacga tgcagttcct gtgggacgac caaaacctct acgtccgctc
     3961 cgaggtggtg gataacgtct tcgccaatcc caaagacgac gaatcgctgt ggaatcagga
     4021 cggcctgcaa ttcctgttcg acccctaccg cggagaacta cagagccgcg gacggtatga
     4081 ttattccatg gggctgggcc agaagggcct ccaggcgtgg cgccacatgt ccgcatcgcc
     4141 gggcatcaag gccggtcccg cgcccgaaat ccagctcacg gtgacgcccg cgtccaaaga
     4201 caacggcaac cgcacctacg aagtcaccat tccctgggcg cagctggcgc cgttccagcc
     4261 caaagtcggc ggcgacctgg gcacgaccat gatcattaac gaggacgatg gcgagggccg
     4321 caaatccacg atcggctggt atggcggcgt gcacctcaag gaagccgcgt ttgtcggcga
     4381 cttgattctt gccgaataag cgcgcaaata tatccgtccc agccgccaga gttcctcgac
     4441 ccacttcctc atgactgccg ccgtttccgc cattcacttc gattccgagt cccgcagttt
     4501 tgtcctgcgg ctgaaaacca gcgcctatct cctgcgaatc ctgcccgaag gcgcggtccg
     4561 gcacgtgggt tttgtgcccc tgccgcccga ggcgcccgcc acgctcgccg cggcgcattt
     4621 gacggaatac aaggacgcca acttcgtctg ggactggcag ggaacgccgt ttgaatatcc
     4681 ggccttcggc gatgtttcct accgcgaggc cgccttcaag gtggcgttca cgcaggcact
     4741 ggcggagttg accgcggagg aggctccgca cgtcccggtc cgcgacgtgc ggctgcgtta
     4801 cgagcgccac gaaatccacc cggatgccga accgctgggc gcgcccgcgc atgggcatcc
     4861 cccgcaggtg gccacgccgc gcgaaaccct gtgcctccgc ctgaaggacg agctcggcga
     4921 tttttacgtc accctgtttt accgcctgac gccggagcag gatctcatcg agcgctgggt
     4981 cgaggttgag aaccggacga atgcgcccgt cgagatcgaa accgtggatt ttggcgtgtt
     5041 gaatctgccg tgcggccatt acgaattgac ccgcgccgcc ggcgcctggg cgcgcgaatt
     5101 cgcgccggtc acccagccga tcgaacaggg ggttttcgtg ctcgaccagc gtggattgaa
     5161 taccggccac gccgtcaatc ccttcttcca cgtctgtgaa cgcggccagg ccaccgagga
     5221 ggcgggaacg gtctatttcg gcgcgatcgc ctggagcggc aactggcggc tgcgcttcga
     5281 atccctgccc ggcggcaatt gccggatatt cgcgggttat gaagcctccg atttcgccct
     5341 cacccttggg cccggcgagt cgcacaagac gccggccttc ctgttcggct gcagctccga
     5401 gggacggggc ggcgccagcc ggaggctgca tcgtttcctg cgcgagcgcg tgctgcccgg
     5461 ccatggcccg gaggagttcc ggcccgtgct ttacaatagc tgggaggcca cctacttcga
     5521 tctctcgctg gaaaaccaga ccgagctggc ccgggtcgcc gccgacatcg gcgtcgaact
     5581 cttcgtggtg gacgacggct ggttcggcgc ccggcggcat gaccgggcgg gcctgggcga
     5641 ctgggttgtc agccaggatg tctttcccgg cggcctgcag ccgctggccg acgaagtgcg
     5701 ccggctggga atgaagttcg gcctctgggt tgagcccgag atggtcaatc ccgattccga
     5761 cctctaccgc gcccatccgg actgggtgct ccattttccc gggcgcccgc gcacgacgag
     5821 ccgcacgcag atgatcctcg atttcggccg ccccgaggtc gtgcaatatc tcctcgatgc
     5881 gctcgacgcg ctggtgcgcg aaaacggcgt ggattttttc aagtgggaca tgaaccgcta
     5941 cgtctccgag cccggctccg tggccggcaa ggcgatctgg cgcaaacacg tggaaggcct
     6001 ctaccgcatc tgggacgccc tgcgccgcgc ccatccgcat ctcgacatcc agagctgctc
     6061 cggcggcggc ggccggattg atccgggcat cctcgcccgc gccgaccagg tctgg
//
LOCUS       DATDAY010000102         2236 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_583580, whole genome shotgun sequence.
ACCESSION   DATDAY010000102 DATDAY010000000
VERSION     DATDAY010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2236)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2236)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2236
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_583580"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(113..424)
                     /locus_tag="VIS74_01970"
     CDS             complement(113..424)
                     /locus_tag="VIS74_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEY5792034.1"
                     /translation="MNIAKMLKQAQQMQSQLAETQSKLAEKTVEASSGGGKVTVTANG
                     AGDVTAIRIDPAIVSVGDVEMLEDLVLVAVKQAIANAKKMAGDEMGKITSGLNIPGLP
                     F"
     gene            complement(431..2035)
                     /gene="dnaX"
                     /locus_tag="VIS74_01975"
     CDS             complement(431..2035)
                     /gene="dnaX"
                     /locus_tag="VIS74_01975"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEY5792035.1"
                     /translation="MSYRVFARKYRPQVFEEVVGQEHITRTLQNAIRQNRLAQAYLLV
                     GPRGTGKTSTARILAKALNCEKGSTPNPCGECVSCREIAEGNSLDVLEIDGASNNSVD
                     NIRELRENAAYAPAKGPYKVYYIDEVHMLSAGAFNALLKTLEEPPPHVKFLFATTEVQ
                     KVPATIASRCQRFDLRRIPDDRIAKHLLQIAEKEKIEIEETAVQAIARGADGGLRDAE
                     SMLDQVVAFCGERISAADVFEVFGFTPAEVVESLAARILAADAPGALEVVNAQSDAGK
                     DLAQLLGGLIVQFRDTLVGQVRGAAAPGVSRDKLLDLLEHLADSESRMKWAPDKKLQL
                     DVAVIKAVHLIEQASLSEVIDALSDLRGGVLPSLPSPPAAPAAAKPTPPEPVAPPPAK
                     PAPVEIKVASVDPKQAWEQTVHELSAASAFKYAWLEHGNFLDEVNGVFRAEYPAEHES
                     MQESPWWMDILSELAAKLSTRTGIQGCRVEFRFSRAEPPAAPPAPAPAAPEAKEAPPV
                     DPMAEFRKDPLIQKALAMFKAEIVTP"
BASE COUNT          383 a          700 c          686 g          467 t
ORIGIN      
        1 gtttctgatt cggaaacccc atttcaatcg gccgggatca gcgatcccgg ctacagggtt
       61 cctgccggcg gtcgatgacc gccgatttcg gaaatttctt ctgagaatcg ctctaaaacg
      121 gaaggccggg aatattgagg ccggaggtga ttttgcccat ctcatcgccc gccatttttt
      181 ttgcgttggc gatcgcctgc ttcaccgcga cgagcacgag atcttcgagc atctccacgt
      241 cgccgacgct cacgattgcg ggatcaatcc ggatcgcggt cacgtcgccc gcgccgttgg
      301 cggtcacggt gaccttgccg ccgccggagg aggcctccac ggtcttctcc gccagtttgg
      361 attgggtttc ggccagctgc gattgcatct gctgggcctg tttgagcatt ttggcaatat
      421 tcatgatttt ttatggggtg acgatttccg ccttgaacat ggcgagcgcc ttctggatga
      481 gcggatcctt gcgaaattcc gccatcgggt caaccggcgg cgcctccttg gcctccggcg
      541 cggctggggc gggagcgggc ggagcggcgg gaggttccgc gcgggaaaac cgaaattcca
      601 cgcggcagcc ctggatgccc gtgcgggtgc tgagcttggc ggccagctcg gagagaatgt
      661 ccatccacca cgggctttcc tgcatggatt cgtgctccgc gggatattcc gcgcggaaaa
      721 ctccgttgac ttcgtccagg aaattgccgt gctccagcca ggcgtattta aaggccgaag
      781 ccgcggataa ttcatgcacg gtctgctccc aggcttgctt cggatccacc gaggcgacct
      841 ttatttccac cggcgcgggc ttggccggcg gcggcgccac gggttcaggc ggggttggtt
      901 tggccgccgc gggagcagcc ggcggcgatg gcaaactggg caggactccg ccgcgcaagt
      961 cggagagcgc gtcgatgacc tcgctcaggc tggcctgctc gatcaagtgc accgccttga
     1021 tgacggccac gtcgagctgc aattttttgt ccggcgccca cttcatgcgg ctctcggaat
     1081 cggccaggtg ttcgagcaga tcgagcagct tgtcccggga aacacccggg gccgccgctc
     1141 cccgcacctg gcccaccagg gtgtcgcgaa attggacgat caggccgccc agcaattggg
     1201 ccagatcctt gcccgcgtcg ctttgggcgt tcaccacctc cagcgcgccc ggcgcgtcgg
     1261 cggccaggat gcgggcggcc agcgattcca ccacctcggc cggcgtgaat ccaaacactt
     1321 caaacacgtc cgccgcgcta atccgctccc cgcaaaaagc caccacctga tccagcatgg
     1381 actcggcgtc gcgcaggccg ccatccgctc cccgggcgat cgcctggacc gcggtttcct
     1441 cgatctcgat tttttccttt tccgcgattt ggagcaggtg tttcgcgatg cggtcgtccg
     1501 gaatccggcg caggtcgaac cgctggcagc ggctggcaat ggtcgcgggc accttctgca
     1561 cctcggtggt cgcaaacaaa aacttcacgt gcggcggcgg ctcctccagc gtcttgagca
     1621 gggcgttgaa ggccccggcg ctgagcatgt gcacctcgtc aatgtaatag accttatacg
     1681 gccccttggc cggcgcgtag gcggcgtttt cgcgcagctc gcgaatgttg tccacgctgt
     1741 tgttgctcgc gccgtcgatt tccagcacgt ccaggctgtt gccttcggcg atctcccggc
     1801 aggagacaca ttcgccgcag ggattcgggg tcgagccctt ctcgcaattc agcgccttgg
     1861 ccagaatccg cgccgtggag gtcttgcccg tgccgcgcgg cccgacgagc aaataggcct
     1921 gcgccaggcg gttctgccgg atcgcattct gcaaggtccg cgtgatgtgt tcctggccca
     1981 cgacttcttc gaagacctgg ggacgatatt tccgcgcgaa aacccgatag ctcaccgtca
     2041 aagagtgatt aaaccaccgg ggttttacaa gattttcggc aaaggttttc gtccgccgat
     2101 ttcgccgatt tttttgacat gtgcgtcaaa cgcccagcga ggctcgcgcc atttgagagc
     2161 atcgcggaca tgaaggacat gaagaaaaaa acgaggacat tttgaccgcg gatgaagctt
     2221 taaaatatcc gcttta
//
LOCUS       DATDAY010000103         2595 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_62034, whole genome shotgun sequence.
ACCESSION   DATDAY010000103 DATDAY010000000
VERSION     DATDAY010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2595)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2595)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2595
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62034"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            131..715
                     /gene="pgsA"
                     /locus_tag="VIS74_01980"
     CDS             131..715
                     /gene="pgsA"
                     /locus_tag="VIS74_01980"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEY5792036.1"
                     /translation="MDATFNPMTLPNLITLFRLFLIPVFVLFAVYYGRSVATGSPHEW
                     QRLAAIIVFIVAAVSDCLDGYLARKWHQQSKLGTVLDPIADKGLLLTAIITLTLSNWT
                     YMFPLWFPVVVIARDVFIVVGYFVLRHFNSHLEVRPSLLGKSATAAQMVALAWVMLQL
                     PFHLYPIWIAGILTVISGLGYIIGGMRQLHPHDS"
     gene            858..2192
                     /gene="der"
                     /locus_tag="VIS74_01985"
     CDS             858..2192
                     /gene="der"
                     /locus_tag="VIS74_01985"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEY5792037.1"
                     /translation="MRTVAIVGRPNVGKSALFNRLAGRRISIVHDQPGVTRDRLGAVC
                     KLGSAPFEIVDTGGIGWAADEEFHEATRTAAEAAIASADVILFVVDGLAGLAPLDREL
                     AEKLRQSGRAVLLVVNKMDTAKHEAQAVEFAALGFDEMRIVSAAHGRGISELVAAAEA
                     KLPPGEALPEGHEPPRLAVVGRPNVGKSSLVNALLGDSRTIVSEVAGTTRDAVEVLWK
                     WNGVDYLLCDTAGLRHRRKHSTSVEVFSVMRTEEVIKRADVCVLVIDATEGVTAFDKK
                     IAGLIQEAQKAAIIVLNKWDLLPGEGREEHIAAVRRQLFFLDYAPVVVLSAKLGTQVK
                     RLHSTVQRVREQARQRCGTGELNRLLKRAMEQQAPPSKGSRSFKLLYATQAGSEQPRP
                     FAPPTIVCFVNAAALMTDPYQTYLIRQIRAKIPYEGLPIRLRYRNREAKDRE"
     gene            complement(2245..>2595)
                     /locus_tag="VIS74_01990"
     CDS             complement(2245..>2595)
                     /locus_tag="VIS74_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY5792038.1"
                     /translation="FLFRKEEVYKKTHVLSGGEKTRLNLIKFLVDPPNILLMDEPTTH
                     LDILTVESLILALENYEGTLVFISHDVHFIRKLATKVLHVNAGKIKAYPGGYDYFLEK
                     VGGLDNERAALTAG"
BASE COUNT          459 a          712 c          899 g          525 t
ORIGIN      
        1 gatttccgaa aaaggattca atgcaggggc ggttgtgcca accgcccagt gttaccaaag
       61 gcgcttggca caagcgcctc tgcaatgcat tgggtatttc ggaaatttct tttgagaatc
      121 actctaatga ttggatgcga cctttaatcc gatgacgctg ccgaatctca tcacactttt
      181 ccgcctgttc ctcattccgg tctttgtgct gtttgcagtg tattacggcc gcagcgtcgc
      241 caccggttcg ccgcacgaat ggcagcgcct ggccgccatc attgtcttca tcgtggccgc
      301 ggtctcggat tgcctggacg gctatctcgc ccgcaaatgg caccagcaaa gcaagctcgg
      361 caccgtgctg gatcccatcg ccgacaaggg gctgctgctg acggccatca tcacgctcac
      421 gctcagcaac tggacctaca tgtttccgct gtggtttccg gtcgtcgtca tcgcgcggga
      481 tgtgtttatc gtggtcgggt atttcgtgct ccggcatttt aattcccacc ttgaggtgcg
      541 ccccagcctg cttggcaaat ccgccacggc cgcgcagatg gtcgccctcg cgtgggtgat
      601 gctgcaactg ccgttccatc tttatcccat ctggatcgcc gggattctga ccgtgattag
      661 cggcctgggc tacatcatcg gcggcatgcg gcagctgcat ccgcacgaca gttaggttca
      721 cgcaaagacg cgaaggcgca aagcaagccg gcgggttggt tggggattag ttatgcgtgt
      781 gaatccgtgt ccatcggtgg ttcggatgcc tccggggttg atccggcggg ggtttccggt
      841 taggcagtgg aggttctgtg agaactgttg cgatcgttgg ccgcccgaat gtgggcaaat
      901 cggctttgtt taaccgcctg gccgggcgga gaatttccat tgtgcatgat cagccggggg
      961 tgacgcgcga ccggctgggg gcggtttgca agctgggctc agcgccgttt gagatcgtgg
     1021 acacgggcgg catcggctgg gcggcggatg aggaatttca cgaggcgacg cgcaccgcgg
     1081 cggaggcggc gatcgcctcg gcggacgtga ttttattcgt ggtggacggc ctggcgggcc
     1141 tggcgccgct ggaccgggag ctcgcggaga aattgcgcca gtccggtcgg gcggtcctgc
     1201 tggtggtgaa caagatggac acggcgaaac acgaggcgca ggcggtggag tttgccgccc
     1261 ttggtttcga cgaaatgcgg atcgtaagcg cggcgcacgg ccggggaatt tccgagttgg
     1321 tggcggcggc ggaggcgaag ctgccgccgg gggaggcatt gccggaaggc cacgagccgc
     1381 cgcggctggc cgtggtgggc cggccgaatg tgggcaagag ctcgctggtg aacgcgctgc
     1441 tcggagattc gcggaccatc gtcagcgagg tggccgggac aacgcgcgat gcggtggagg
     1501 tgctctggaa atggaacggc gtggattatt tgctctgcga cacggcgggg ctgcggcatc
     1561 ggcgcaagca cagcacgtcg gtggaggttt tcagcgtgat gcggaccgag gaggtcatca
     1621 agcgggcgga cgtgtgcgtg ctggtgattg acgcgacgga aggcgtgacg gcgttcgaca
     1681 agaagatcgc gggattgatc caggaggccc agaaggcggc gatcattgtg ctgaacaaat
     1741 gggatttgct gccgggcgag gggcgggagg agcacatcgc cgcggtccgg cgccaattgt
     1801 ttttcctcga ttacgcgccg gtcgtggtgc tgtcggccaa gctgggcacg caggtcaagc
     1861 ggctgcattc gaccgtccag cgcgtgcgcg aacaggcgcg gcagcgctgc ggcacggggg
     1921 agctgaaccg cctgctcaag cgggcgatgg aacagcaggc gccgccgagc aagggcagcc
     1981 ggagcttcaa gctgctctac gccacgcagg cgggcagcga gcagccgcgc cccttcgcgc
     2041 cgccgaccat cgtgtgtttt gtgaatgcgg cggcgctgat gaccgacccg tatcagacct
     2101 atttgatccg gcaaatccgg gcgaaaatcc cctacgaggg gctgccgatc cggctgcgct
     2161 atcgcaatcg cgaggcgaag gatcgggagt gacgggtgtc ctccgaatga ttgcgccctg
     2221 acgaaggcgg gaaaaccgaa ccgcttaacc cgcggtcagc gcggcgcgct cgttgtcgag
     2281 gccgccgact ttttcgagaa aataatcata gccgcccgga tacgctttga ttttgccggc
     2341 gttcacgtgg aggactttcg tggcgagctt gcggatgaaa tgcacgtcgt gggaaatgaa
     2401 caccagcgtg ccttcgtaat tttccagcgc caggatcagg gattccaccg tcaggatgtc
     2461 gagatgcgtg gtcggctcgt ccatgaggag gatgttcggc ggatcgacga ggaatttgat
     2521 gaggttcaat cgcgtttttt ccccgccgct gagcacgtgg gtctttttgt aaacctcctc
     2581 cttgcggaaa aggaa
//
LOCUS       DATDAY010000104         2969 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_2142082, whole genome shotgun sequence.
ACCESSION   DATDAY010000104 DATDAY010000000
VERSION     DATDAY010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2969)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2969)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2969
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2142082"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..436)
                     /locus_tag="VIS74_01995"
     CDS             complement(<1..436)
                     /locus_tag="VIS74_01995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792039.1"
                     /translation="MRAFAGGSRVLRWIIAAAALAMPLHAAEVTVVGYNVQNYLLMDR
                     QVDGQKVKDAPKPENEVTALIHVITQLKPDILGLCELGGSAQLADLQNRLKQAGLDLP
                     HAEWIQGADTDRHVVLLSRFPIVARNSVSDIPLTLNGQAAGMQ"
     gene            complement(455..691)
                     /gene="tatA"
                     /locus_tag="VIS74_02000"
     CDS             complement(455..691)
                     /gene="tatA"
                     /locus_tag="VIS74_02000"
                     /inference="COORDINATES: protein motif:HMM:NF014470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HEY5792040.1"
                     /translation="MMTPLIAFGSLGWPEILLIFLVVLVLFGAKRLPELARGLGRSLS
                     EFRKAKDEFDQEIRKSAQELQSEKPAETHPSKQA"
     gene            complement(932..1867)
                     /locus_tag="VIS74_02005"
     CDS             complement(932..1867)
                     /locus_tag="VIS74_02005"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEY5792041.1"
                     /translation="MSSDSKAFTEAAVWQSIQGEWRPLYGAFSERGISVEWHDFACED
                     ALDWSRSFHPQSLELCVNLTGCGSIGQGRNRTIVNPQSVHLYGTTGQPLAADRHAAQR
                     HQFLTIEMSGGWLQRQLGWAMEKANPTAVSFLDGRKRPQPSTTALSSRVRRLAEQLLD
                     PPHAGIAREVWYHAKVIELLAQSLFSEPGEELFCQRQKRVARERVERVKELLGADLEN
                     PPSLTELSRSIGCSAFYLSRIFSQETGTTLTQYLRKLRLGRAAELLQTGRYNVTEAAL
                     AVGYNSLSHFSKAFSEMFGCCPCVYPLGARRAARG"
     gene            1961..2338
                     /locus_tag="VIS74_02010"
     CDS             1961..2338
                     /locus_tag="VIS74_02010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HEY5792042.1"
                     /translation="MTETAPAAPQYRIGNEKLIRITAPASAKLRSLLERQGRLERGAL
                     RVAVIGGGCSGLQYKMDLVDGPANRDILVESNAVRVVIDPKSALFVSGSELDFSDDLQ
                     KGGFKVTNPNAVAHCSCGESFSA"
     gene            2348..2815
                     /locus_tag="VIS74_02015"
     CDS             2348..2815
                     /locus_tag="VIS74_02015"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HEY5792043.1"
                     /translation="MFDAFAAMGVSRHPWVDAAGLKEKFREAAAKLHPDSPGGDTAAF
                     HDLNEAFAVLRDPARRLRHLAALAYPEFAGKAAFTPNADLFARVHSALANGDLAAVKA
                     LQQELGARLKRLDESLPQIPPDPQKYLTAAAEYTFLQKWSDQLRERAFRLSNR"
BASE COUNT          555 a          904 c          930 g          580 t
ORIGIN      
        1 gctgcatgcc tgcggcctgg ccgttgaggg tgaggggaat gtcgctcacg gagttgcgcg
       61 cgacgattgg aaaacggctc aagagcacga cgtgccgatc ggtgtcggcg ccctgaatcc
      121 attccgcatg gggaagatcc agcccggctt gtttcaaccg gttttgcaga tcggcgagct
      181 gggcgctgcc acccagctcg cagaggccca ggatgtcggg tttgagctgg gtgatgacgt
      241 ggatgagtgc ggtgacctca ttttcgggct tgggcgcgtc cttgaccttt tggccatcca
      301 cctgccggtc catgagcaga taattttgga cattgtagcc caccaccgtg acctcggcgg
      361 cgtgcagggg catcgccaag gcggcggcgg caataatcca tcgcaaaacc cggcttccgc
      421 cggcgaaggc tctcatggga ggcgcggaaa agaatcaggc ctgcttggag ggatgggtct
      481 cggcgggctt ttcggactgg agctcctggg cggatttgcg gatttcctga tcgaattcat
      541 ccttggcctt gcggaactcg ctcaggctcc ggcccagccc gcgggccagt tcgggcaacc
      601 gcttggcgcc aaacaatacc agcaccacca aaaagatgag caggatttcc ggccagccca
      661 gcgaaccaaa tgcgatgaga ggagtcatca ttgaattaaa ctatcacgcc ccgccaaagt
      721 tgcaaagaaa agcaatgagg agccctggga tgtttttttc tcccgcgaag gcgcggaggc
      781 gcgaagagtt tggatcgtaa gggcggctta atgagctgca ccgctccttt ttagagaagg
      841 cggcgaagca tcgaaaccgc ccgcggtccc cctctttgct atctttgtgt gcttctgttt
      901 aaaaacctcc gcgccttcgc gggggataaa attaaccgcg ggccgcccgg cgcgcgccca
      961 gcggatagac gcacgggcag cagccgaaca tttcgctgaa ggctttgctg aaatggctga
     1021 ggctgttata gcccacggcc agagccgctt cggtgacatt gtatcggccg gtttggagca
     1081 gctccgccgc ccgtccgaga cggagtttgc gcaggtattg cgtcagcgtc gtgccggttt
     1141 cctgcgaaaa aatccggctc aaataaaacg cgctgcagcc gatgctccgg ctgagttcgg
     1201 tcaggctggg cggattttcc agatccgctc cgagcaattc cttcacccgt tccacccgtt
     1261 cgcgggccac gcgtttctgc cgctggcaga acaactcctc gccgggttcg gaaaaaaggc
     1321 tttgcgcgag cagttcaatg accttggcat gataccagac ctcgcgggcg atgccggcgt
     1381 gcggtggatc cagaagctgt tccgcaagcc ggcggacgcg gctggagagc gccgtcgtgg
     1441 agggttgggg gcgcttgcgt ccgtccagaa acgagaccgc agtcgggttg gccttctcca
     1501 ttgcccagcc cagctgccgt tggagccagc cgccggacat ttcgatggtc agaaattgat
     1561 gccgctgcgc ggcgtggcgg tcggcggcga gaggttgtcc ggtggtgccg taaaggtgga
     1621 cgctttgcgg attgacaatg gtgcggttgc ggccctggcc gatcgaaccg cagccggtga
     1681 gattcacgca gagttcgagg ctttgcggat ggaaactccg cgaccagtcc aaggcgtcct
     1741 cgcaggcgaa gtcgtgccat tctacgctga tgccgcgctc cgagaacgca ccgtaaagcg
     1801 gccgccattc tccctggatg gattgccaga cggcggcttc ggtgaacgct ttggaatcgg
     1861 aactcattcg actggcaaag ctcatatttg agaatcagtc tcaaaagcaa ggaaaaagcg
     1921 acttcttgaa agttgttccg cgcccgcgta agctcccggc atgactgaaa ccgccccggc
     1981 ggccccgcaa taccgcattg gcaacgaaaa gctcatccgc atcaccgcgc cggcgagcgc
     2041 gaaactgcgt tccctgctgg agcgccaggg ccggctggaa cggggcgcgc ttcgggtggc
     2101 ggtcatcggc ggcggctgtt cgggactgca atataagatg gacctcgtgg acggcccggc
     2161 caatcgcgac atcctcgtcg aatccaacgc ggtgcgcgtg gtcattgatc ccaagagcgc
     2221 cctgtttgtg agcggatcgg aactcgattt ttccgacgac ctgcaaaagg gcggcttcaa
     2281 agtcaccaat ccgaatgccg tggcgcattg ctcctgcggc gaaagttttt ccgcatgatg
     2341 aattccgatg tttgatgcct tcgccgccat gggcgtttcc cggcatccct gggtggacgc
     2401 cgccgggttg aaggaaaaat tccgtgaggc cgccgcgaaa ctgcatccgg attcgcccgg
     2461 gggcgacacc gccgcgtttc acgacctgaa cgaggcgttc gccgtccttc gcgatcccgc
     2521 ccgccgcctg cgccatctcg cggcgctggc ctacccggaa tttgccggca aggccgcctt
     2581 cacgcccaat gccgacctgt ttgcccgcgt ccattccgcc ctggccaatg gcgatctcgc
     2641 cgccgtgaag gccctccagc aggaattggg cgcccggctg aagcggctgg acgaatccct
     2701 gccgcaaatt ccgcccgacc cgcaaaaata tctcaccgcc gccgccgaat ataccttcct
     2761 gcaaaaatgg agcgatcaac tccgcgagcg cgcgttccgt ttgagcaatc gttagcgggc
     2821 aacgccgccc cgcgaatcgg tccgctggca gggtggcgtt gccaatcccc gggcgtagtc
     2881 gcatcaggcg tgaattcacg ctgcgggttc ccctttttta tttttggaat gggcacgcgc
     2941 ggcgcgcctg ccctaccgtg ctggtcatg
//
LOCUS       DATDAY010000105         2643 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_2170365, whole genome shotgun sequence.
ACCESSION   DATDAY010000105 DATDAY010000000
VERSION     DATDAY010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2643)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2643)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2643
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2170365"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..647)
                     /locus_tag="VIS74_02020"
     CDS             complement(<1..647)
                     /locus_tag="VIS74_02020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792044.1"
                     /translation="MSKSSGPPTPNPASIGSPRIRLAGLLGLLFLTAATAWPATVVKP
                     LKPLNRSVSPSRQFVVYCADAKIRLPLTALADDAAVQWQDWLGEKESWKTPIILNFPE
                     DARGRARPGVRTTLAEADGGQSKVQVDIQDPSLAFSPELRAEIFRALALEYSYRGAAQ
                     KSGKSYRLAPDWLAEGLAEEMRSRQDGVPARVVEGLLNAPKKPSVMDLLRGRPPTL"
     gene            complement(616..1365)
                     /locus_tag="VIS74_02025"
     CDS             complement(616..1365)
                     /locus_tag="VIS74_02025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEY5792045.1"
                     /translation="MILNTCPSCGFANDATRVFCHQCNSKLNRLDPAAKPLPPPVPAP
                     RRPAKPAAGEGFRPVSLIFSIVRIGIFAALLAALLLCLRPPADIPAPAQPDPALVAAL
                     QDRYAKTVASSPGPRQLILTSEELNTTFATQVTVRQNSSLAALSTSTRPFLALNHGTF
                     TLGLEILAVGQRVVLQSEFAVEGNPGHLTARMVRAQIGHLVIPPPVAPLIMRWTQTFA
                     NAFSRQLQEIEKAEKVVIGPGNVQIIWPPNP"
     gene            complement(1362..1634)
                     /locus_tag="VIS74_02030"
     CDS             complement(1362..1634)
                     /locus_tag="VIS74_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdB family zinc ribbon protein"
                     /protein_id="HEY5792046.1"
                     /translation="MPTYEYECQKCRKTFDVFQSMKDEPLKTCPDESCEGGLKRLLGT
                     GAGLLFKGSGFYITDYRSEGYKKAAKKDGGTDSAPAKPTTPKSDGK"
     gene            1726..>2643
                     /locus_tag="VIS74_02035"
     CDS             1726..>2643
                     /locus_tag="VIS74_02035"
                     /inference="COORDINATES: protein motif:HMM:TIGR03718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC/Alx family metal homeostasis membrane
                     protein"
                     /protein_id="HEY5792047.1"
                     /translation="MTAPILASMPFQWWALFFLIVAVLLALDLGIFQRKTHQLKMGEA
                     LVAVGFRVALALLFNLAIYLGWIGNYPTPAAQHQAGLEFLTGYLVEIALSVDNVFVFA
                     LVFKYFKVPGEYQHRVLFWGILGALAMRAVMIFAGVKLLESLSWINYVFALILIYGGI
                     KMIKDDKDDLDPLQNPLVRLVRRIFPVTPELRGQHFFVRQEGKFFATPLLVVLVAIES
                     TDLVFAVDSIPAVLAVTTDFFLVFTSNIFAILGLRALYFALAGVMDLFRYLHYGLSAI
                     LVFIGVKLALAHTPFKIETVQSLIVVFALL"
BASE COUNT          442 a          791 c          864 g          546 t
ORIGIN      
        1 agggtcggcg gccgcccgcg caggagatcc atgaccgagg gctttttcgg cgcgttgagc
       61 agcccttcga cgacccgggc cggcacgccg tcctggcgcg aacgcatttc ctccgccagc
      121 ccctcggcga gccagtccgg agcgaggcga taggatttgc cggatttctg ggcggcgccg
      181 cgatacgaat attccaaagc cagcgcgcga aagatttccg cccgcagctc ggggctgaac
      241 gccagggagg gatcctggat atccacctgc accttcgact ggccgccatc cgcttccgca
      301 agcgtggtgc gaactcccgg gcgcgcgcgt cccctggcat cctccgggaa attgagaatg
      361 atcggggtct tccacgactc cttctcgccc agccagtctt gccactggac cgcggcgtca
      421 tccgccagcg cggtgagcgg gaggcggatt ttggcatcgg cgcagtaaac cacgaattgc
      481 cgggacgggc tgaccgaacg gttcagcggc ttgagcggct tgaccaccgt ggccggccag
      541 gcggtggcgg ccgttaaaaa aagcaggccg aggaggccgg ccaggcggat tcggggggaa
      601 ccgatcgagg cgggattagg ggttgggggg ccagatgatt tggacattgc cggggccaat
      661 gaccactttc tcggcttttt caatttcctg aagctgccgg ctgaaggcat tggcgaatgt
      721 ttgggtccag cgcatgatca gcggcgcgac cggaggcggg atcacgaggt ggccgatctg
      781 ggcccggacc atgcgggcgg taagatggcc cggatttccc tccaccgcaa attcggattg
      841 gagcaccacg cgttgcccga cggcgaggat ttccaggccg agggtaaacg tgccgtgatt
      901 gagagccagg aagggccggg tgctggtgga gagggccgcc agcgagctgt tctgccggac
      961 cgtcacctgg gtggcgaaag tggtgtttag ttcttccgag gtcagaatga gttgccgcgg
     1021 gccggggctg gacgccaccg ttttggcgta acgatcctga agggcggcca ccagcgcggg
     1081 gtcgggctgg gcgggcgccg ggatatccgc cggcgggcgc aggcacaaga gcagcgcggc
     1141 cagcagcgcg gcgaagatcc cgattcgaac gatcgaaaaa atcagggaaa cgggccggaa
     1201 tccctcgcct gccgccggct tggcggggcg gcggggcgcg gggaccggcg gcgggagcgg
     1261 cttggccgcc ggatccaggc ggttcagctt ggagttgcat tggtggcaaa atacgcgggt
     1321 ggcatcgttg gcgaagccac agctggggca ggtgtttaag atcattttcc gtcgcttttt
     1381 ggagtggtgg gtttggcggg ggcggaatcg gtgccgccgt ccttcttggc ggcctttttg
     1441 tagccttcgc tgcggtagtc cgtgatgtaa aagccggagc ctttgaaaag gagccccgcg
     1501 ccggttccga gcagccgctt gagcccgccc tcgcagcttt catccggaca cgtcttcagc
     1561 ggctcgtcct tcatggactg aaagacgtca aacgtcttcc ggcacttctg gcattcgtat
     1621 tcgtaggttg gcatacaaat cgagaaccca tagcggcttt ccgattttcc cgcaatagcg
     1681 cgcttgcccg ccgcgcggaa tcccgctaaa gccctctggc gtgtgatgac agctccgatt
     1741 ctcgcttcca tgccctttca atggtgggcg ctcttttttc tcatcgtcgc agtcctgctg
     1801 gcgctggatc tgggcatctt ccaacgcaag acccatcaat taaaaatggg cgaagcgctg
     1861 gtcgcggtcg gcttccgcgt ggccctggcg ctgctgttta atctcgccat ctacctcggc
     1921 tggatcggga attatcccac ccccgccgcg cagcaccagg ccgggctgga atttctcacc
     1981 ggctacctgg tcgaaatcgc gctgagcgtg gacaacgtct tcgtctttgc gctggtcttc
     2041 aaatacttca aggtgccggg cgaataccag caccgcgtgc ttttctgggg catcctcggc
     2101 gcgctggcca tgcgcgcggt catgatcttc gccggcgtga agctgctcga atcgctaagc
     2161 tggatcaact acgtgttcgc gctgatcctg atctatggcg gcatcaagat gatcaaggac
     2221 gacaaggacg acctcgaccc cctgcagaat cccctggtcc ggctggtgcg ccggattttt
     2281 cccgtcaccc cggaactgcg ggggcagcat ttttttgtcc gacaggaagg taaatttttc
     2341 gccacaccgc tgctggtcgt gctggtcgcg attgaatcca cggacttggt cttcgcggtg
     2401 gattcgattc ccgcggtgct cgcggtgacc acggatttct tcctcgtgtt cacctcgaat
     2461 attttcgcca tcctgggcct gcgggcgctc tacttcgcgc tggccggcgt catggatctt
     2521 ttccgttacc tgcactacgg attgtccgcg atcctggtat tcatcggcgt aaagctcgcg
     2581 ctggcgcata cgccgttcaa aatcgagacg gtgcaatctt tgatcgtcgt cttcgcgctg
     2641 ctc
//
LOCUS       DATDAY010000106         2194 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_1989393, whole genome shotgun sequence.
ACCESSION   DATDAY010000106 DATDAY010000000
VERSION     DATDAY010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2194)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2194)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2194
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1989393"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..466
                     /locus_tag="VIS74_02040"
     CDS             <1..466
                     /locus_tag="VIS74_02040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792048.1"
                     /translation="GSMAMNYYTTPRMTRDIDMVVELSASDAEKMASAFSPDYLAESA
                     AIEDAIRHQGMFNLIHLESIIKVDCIIRKESPYREHEFARRQRANLGGFETWIVSKED
                     LIISKLDWAKESHSERQMDDIQNLLASGYDADYLGEWIKRLGLTSIWNMIPK"
     gene            484..726
                     /locus_tag="VIS74_02045"
     CDS             484..726
                     /locus_tag="VIS74_02045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792049.1"
                     /translation="MAQIYRERLMALSPAERWERGARMFDAARTMVLASFPPHLSITE
                     IRLKLLQRFYPELDTEPVRRAILAKVNKGSPDQFPT"
     gene            1714..1944
                     /locus_tag="VIS74_02050"
     CDS             1714..1944
                     /locus_tag="VIS74_02050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792050.1"
                     /translation="MVCRKGFLTNEEVRSFLEGEGFQAAVNSYFGWYRLADGVLIVDA
                     KPDNFILTECGIVPIDLQMALVDPGKLRAAFL"
     gene            complement(1925..>2194)
                     /locus_tag="VIS74_02055"
     CDS             complement(1925..>2194)
                     /locus_tag="VIS74_02055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792051.1"
                     /translation="SSLIAYLRGAWNQGKAGGSMGCGWGGGAPGDFGIEAEPVNPSAK
                     GTNADDAGRTCVRAINLRRITRLMGQVMNKNITLRLFDFSTGTPL"
BASE COUNT          489 a          614 c          607 g          484 t
ORIGIN      
        1 gggatcgatg gcgatgaact attacaccac gcctcgcatg acccgcgaca tcgatatggt
       61 cgtggagcta tccgcttcgg atgctgaaaa aatggcgtcc gcattttcgc cagactacct
      121 cgctgaatcc gccgccatcg aagacgccat ccgccatcag ggcatgttca acctgatcca
      181 tttggagagc atcatcaagg tggattgcat cattcgcaaa gagtcgcctt accgggagca
      241 cgaattcgcc cggcgccagc gagccaatct cggcggcttc gagacatgga ttgtcagcaa
      301 ggaagacctc atcatttcaa agctcgattg ggcaaaggaa agccattcgg aacgccagat
      361 ggatgacatt caaaacctgc tcgcctctgg atacgatgcc gattatctcg gcgagtggat
      421 taaacgactt ggactcacct ccatttggaa catgatccca aaatgaccga tacctcgcca
      481 gagatggccc aaatctaccg cgagcgcctg atggcccttt cacctgcgga gcgatgggag
      541 cggggcgccc gcatgtttga tgccgcccga acgatggtgc tggcttcgtt tcctccccac
      601 ctctcgataa ccgaaatccg cctcaaactc cttcagcgct tctatcctga actggatacc
      661 gaacccgtgc gccgggcgat tttggcgaaa gtcaataagg gaagccctga tcaattcccc
      721 acataaagcc aaccgccgcg cggcggttgg ctaaaattgg acagttattg ctcgcctgga
      781 cgcggcggat cggggaaatt gcgccgtttt tgccttcccg gacgaaggag gcgacggttc
      841 tggcgctcgg atcaagggcc gatccgcagg aggtctcccg ccgcagccgt aaagtctttt
      901 cggttgaatg ggacgcattt ccccggcggg gttggcaact ccgccccacc ataaaccgat
      961 ctcccggcag ggcggcgttg gcaacgccgc cgctcactca gcaatctgaa gcaaagaggt
     1021 tctgataccg aatgcagttg aaagcgggat gaatcgaaat gggacggcac tccgttgccg
     1081 tccgttgctt tccgtgaatc cgtttctttt ctgaaaaggg tataaatcag agttttcttc
     1141 atgcgttttt gacgcttgtg aagaggcgga gagcttggga tgctttcgat ccaaagcttg
     1201 acgtaatgaa acccggcctt acggaggaga aggcgatgaa actccagcct ccatccccgc
     1261 agctcttgca gcgctatctc gagctttcga ggatgccgcg caagcgattg actgcggagc
     1321 aagcgagagc gcaggtccag aagcatttgg cggaagccag gaaagaaatg tcgcggagtg
     1381 ggcgcggcga attggcgcgc tgattcccag tcaggaaatt gacggccttc cgcgggtttc
     1441 gaattccacc agcgaacatg aagtttttta ccgccgccgc gatgatcggg cggtaaaaag
     1501 aacttgggcc ggattttacg gccaggtgcc ggtgattctt gaggggagac ttgagagaaa
     1561 aaatgcgccg ccatccgagt atctgcggag gatggcctta caaatcgaag ttttcctcac
     1621 cgatctcagg ctggagggcg tgtgcatttc cgacaaaacc tccatgatca tcggacagcc
     1681 tcccggtgag ccgtctttcg tcatttctca accgtggttt gccgaaaagg ctttcttaca
     1741 aatgaggagg tccgatcctt ccttgaaggc gaaggattcc aagcagcggt caattcctat
     1801 ttcggatggt atcgccttgc cgatggggtg ctgatcgttg acgccaagcc ggataatttc
     1861 atcctgaccg aatgcggcat tgttcccatc gacttgcaga tggcgctcgt tgatcccgga
     1921 aaactcagag cggcgttcct gtagaaaaat caaacaaacg gagggtgatg tttttgttca
     1981 tgacttggcc cattaaccgg gttattcttc ggaggttaat tgcccggacg catgttctcc
     2041 ccgcatcgtc agcattggtc ccttttgccg atggattcac cggttccgcc tcgattccga
     2101 aatcgccggg cgcgccgccg ccccaaccac accccatgga ccctccggct ttgccttgat
     2161 tccacgcgcc gcggaggtaa gctatgagcg atga
//
LOCUS       DATDAY010000107         2460 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_1638764, whole genome shotgun sequence.
ACCESSION   DATDAY010000107 DATDAY010000000
VERSION     DATDAY010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2460)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2460)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2460
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1638764"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            92..622
                     /locus_tag="VIS74_02060"
     CDS             92..622
                     /locus_tag="VIS74_02060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792052.1"
                     /translation="MKKLSLILLTSAVLAGSALAGTEAKSFKKEIIEVEPTCNFRDME
                     FQIDGFYTGFFGSRGSRLHTGSGGGFGLNFFFARYFGIGYEAAWYSNNGVAEHMPAAG
                     SFFVRYPICSWNLAPYAMVGGGAAWDGTTIGYGNVGGGLEYRFTPNWGVFVDGRYFYG
                     GSGNVGNLRSGLRFAF"
     gene            complement(710..1144)
                     /locus_tag="VIS74_02065"
     CDS             complement(710..1144)
                     /locus_tag="VIS74_02065"
                     /inference="COORDINATES: protein motif:HMM:NF013625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HEY5792053.1"
                     /translation="MKTRWVGLAIALVFAAIGTSYAGNRHRNHYYRGDDCNYSRGYYN
                     HGPVYPRPFFRPPVVVFTTWAPRPVYTRTVVYQTVNPESRYGDSRLARAQARLARLGY
                     YSGYIDGDYGPQTARAIRLYQAENGLPITGRLDKWTRRSLGI"
     gene            1296..>2460
                     /locus_tag="VIS74_02070"
     CDS             1296..>2460
                     /locus_tag="VIS74_02070"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEY5792054.1"
                     /translation="MNPADKLGQTEERFRLALEAVAEGVWDWNLETGEFFFSDALVEM
                     LGYSRNEAPSQISFLKNWLHPDDRVQVIQQIQNLRSSPRPYQSQYRLRTKDGRWKWIL
                     DRGRVVQWDAAGRPLRAIGLHIDISEQKASEALIHLREERLRLATDAAHLGIWDSDLR
                     NQTCFWNARMFEIHDVIAGQSTDAQRDWENYVHPGDAERSRRTVLEALHAQRSFSLIY
                     RMLTPAGELKWLRVMGYVYRDSDGIPTRLVGFILDITTTHQMELALESSQNDLRMVEG
                     LAAMGSWSWDCLCDEVIWSEGLYALFGMDPAESLPNFKEHLRLYTPESWEKLRQSVEN
                     CLQTGDPYHLELEAIRSSGGHFFLAARGMAARDELGKIARLYGTVHEITDQKIA"
BASE COUNT          509 a          665 c          757 g          529 t
ORIGIN      
        1 aggggaattc ctgatggtca aatgatcgta aatttccata cttgatgcgg tatccaaggc
       61 tttgccgggg atattttaac ctccttaacc tatgaaaaaa ctctcactca tcctcctcac
      121 ctccgcggtt ttggcgggct ctgccctggc cggaaccgaa gctaaatcct tcaagaagga
      181 aatcatcgaa gtggaaccca cctgcaattt ccgcgacatg gaattccaga tcgacggctt
      241 ctacaccggc ttctttggca gccggggcag caggctgcat accggttccg gtggcggctt
      301 tggcctgaac ttcttcttcg cccgctattt cggcatcggt tacgaagccg cctggtatag
      361 caacaacggg gttgccgagc acatgccggc ggccggaagc ttcttcgtcc gttacccgat
      421 ctgctcctgg aacctcgcgc cttacgccat ggtcggcggc ggcgcggcgt gggacggaac
      481 caccatcggc tacggcaatg tcggcggcgg gctggaatat cgcttcacgc cgaattgggg
      541 tgtgtttgtg gatggccgct acttctacgg cggttccggc aacgtgggca atctgcgcag
      601 cggcctgcgt tttgccttct gatcgggctt tcccggcgat tgaaactaaa aaaccggaag
      661 cggctgccgc ttccggtttt ttgtttggag atgccttgtt tggagtgcgc tagattccca
      721 ggctgcgccg ggtccacttg tccagccgcc cggtgatggg aagaccattc tccgcctgat
      781 agaggcgaat ggcccgggcg gtttgcggac cgtaatcgcc gtcgatgtag cccgaataat
      841 accccagccg cgccagccgc gcctgagccc gggcgagtct ggagtccccg taacgggatt
      901 cgggattcac ggtctggtaa accaccgtgc gggtataaac cggccggggc gcccaggtgg
      961 tgaatacaac caccggcggt ctgaagaaag gacgcggata aacggggcca tgattataat
     1021 aaccgcgcga ataattgcag tcatctcccc ggtagtaatg attacgatga cggtttcccg
     1081 cgtagctggt tccgatggcc gcgaagacca gggcgatggc aagacccacc caacgagttt
     1141 tcatatgaga aaagctccgc ccattttggg gaaaatgcaa ctgggcggac ggaaattatt
     1201 ccatgttcat tcgcgcttgg ttgtgtttgg ataaagaacg gtatggggga agttcagatc
     1261 actcgctcag ccgctcgaaa gcgataccgt tattcatgaa ccccgccgac aaactcgggc
     1321 aaaccgaaga gcgtttccgg ctggcattgg aagcggtggc ggagggtgtg tgggattgga
     1381 atctggaaac cggcgagttt tttttcagcg atgccctggt ggaaatgctg gggtattcac
     1441 gcaacgaagc gccttcgcag attagttttt tgaaaaattg gctgcatccc gatgaccggg
     1501 tgcaggttat ccagcaaatt cagaatttgc gctcctcgcc aagaccttat cagagccaat
     1561 accggcttcg gaccaaggat ggccggtgga agtggattct cgaccggggc cgggtcgttc
     1621 aatgggacgc cgcaggccgc ccgcttcgcg cgatcggcct gcacattgac atttccgaac
     1681 agaaggcgtc cgaagcgctt attcatttgc gcgaggagcg gttgcggctg gcgaccgacg
     1741 ccgcgcattt gggaatctgg gattccgatc tgcggaacca aacctgtttt tggaatgcgc
     1801 ggatgtttga aattcacgat gtcatcgccg ggcagtccac ggatgcgcag cgggactggg
     1861 aaaattacgt gcatcccgga gatgccgaac ggtcgcggcg gacggttttg gaagccctgc
     1921 acgcgcaacg cagcttttcg ttgatctacc ggatgctcac ccccgcgggc gaactgaagt
     1981 ggctgagggt gatgggatac gtttaccggg attccgacgg gattcccacc cggctggtgg
     2041 gtttcattct ggacatcacg accacgcatc agatggagct ggcgctggaa agcagccaaa
     2101 atgacctccg gatggtcgag gggctggcgg caatgggaag ctggtcgtgg gactgccttt
     2161 gcgatgaggt gatttggtcg gagggcctgt atgcgctctt cggcatggac ccggcggagt
     2221 cactgccaaa cttcaaggag cacctgcggc tatatacgcc ggagagctgg gaaaagctcc
     2281 gccaatcggt ggaaaactgc ctgcaaaccg gcgatcccta tcaccttgag ctggaagcca
     2341 tccgttcgag cgggggacat ttttttctcg cggcgcgggg gatggccgcc cgtgacgagc
     2401 tgggaaagat cgcccgtctt tacgggacgg tccatgaaat cacggatcaa aaaatcgcca
//
LOCUS       DATDAY010000108         3593 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_1962561, whole genome shotgun sequence.
ACCESSION   DATDAY010000108 DATDAY010000000
VERSION     DATDAY010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 3593)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3593)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3593
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1962561"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1944
                     /locus_tag="VIS74_02075"
     CDS             <1..1944
                     /locus_tag="VIS74_02075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792055.1"
                     /translation="YARLPFIAKWSAERALPHTEFELRSLQLEQGVWVANGLKLRDRK
                     SGKTLVEMERGTVRLGVWDLWRRHLGEVRLVNPVLRVSPELMALMGGGGGAAGGNGSA
                     MTLPGWSVDRLVCDYGELYVAGLSKQPVNVTARFAFDWKNLSTGEPGASQVNRLQLWN
                     IAASKSEDYSTPFLRFNIVNLDFSLRQLLERRLALIELNGGFVRIGEEFQALLKASSG
                     GAPQSPAQAAAVWIANQLKVGDIGVRVDDPRPEVADIRFRLNTELTEFPLNDVAGEIA
                     QRPQRIELSNVQIVSPVDPDTRIITLRSVFLNFTLAGLLEQRIDRLTLLQPSIFISQD
                     LFVYMDQMKGADAAEPGQTAPPKPSWRIKELEIRYGRLLLGGEKIGQIGLPLEFEASV
                     RDILLDNLASLQLQTQLTIRPRDFEFAAMQLSMDHLRGDLRFSYPPEQRQSNVVNTLF
                     LDGIRWRQYRGQKLWLSVTFDKQTISALFGGEAYGGQLNGGLAFFFSGNNRWVGWLSG
                     RGVDLKGLTDVIAPQNFRMTGPADFKVLVDASGQKIERLKGDFTGTRNGKMTLSKLDD
                     FLARIPPEWNLLKQSSTRIALEALRDYRYDRVKGDFWFVNRQGVLNLRMEGPTGKRNF
                     EIFLHTDESAEGLWQKKSAKSGS"
     gene            1911..2456
                     /locus_tag="VIS74_02080"
     CDS             1911..2456
                     /locus_tag="VIS74_02080"
                     /inference="COORDINATES: protein motif:HMM:NF018701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1318 domain-containing protein"
                     /protein_id="HEY5792056.1"
                     /translation="MAKKIRKIWFLIPLALAACSAPSVNLATPEPIKVDIDMRLDVYQ
                     HADPNAVAVAAAAASKPKPAPAATPSSAQDPAVRQKNRAAEIQNFKNSRWVGEGREGL
                     LVIRQPLPDGDAGDYIAKVVQQENVDRNRLMREQADAQKKPLAEIQAQAAAEWRNRSF
                     SGEFIEVQQPDGAYKLVQKEG"
     gene            2495..>3593
                     /locus_tag="VIS74_02085"
     CDS             2495..>3593
                     /locus_tag="VIS74_02085"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY5792057.1"
                     /translation="MLNALRRLFLGAALLLAASAGLLLSDLASRTGAASADSAPEKTA
                     RVALLQHASQAILDQGRAGMLAGLAERGWTPGQNLEVRNYNAEGDMPVAQAIAREMAN
                     GGHQLLLTISTPSLQAVANANRATQIPHVFALVTDPVAAGVGVNRENPLDHPAWLAGY
                     GTMQPVAKAFATAREMNPSLRTVGVVWNAAEANSEAQVKLARKVCAALDIQLLEASVE
                     NTAGVAEAAASLGARGAEALWVNGDVTVITAIDSLLAAANKANIPVFTVIPPNVKSGA
                     LFDLGADYFEVGRLGGILAGEILSGKDPAAVPIENVMPEMLALNLRTAGKFSAKWRIP
                     PALRERARLIVNEDGSEQAAPPPKTAAAPNPS"
BASE COUNT          650 a         1141 c         1188 g          614 t
ORIGIN      
        1 tatgcgcggc tgccgttcat cgcgaaatgg tcggcggagc gcgcgctgcc gcacacggag
       61 ttcgaattgc gctcgctgca attggagcag ggcgtctggg tggccaacgg gctgaagctc
      121 cgcgaccgca aaagcgggaa gacgctcgtg gaaatggagc gcggcacggt ccggctgggc
      181 gtctgggatt tgtggcggcg gcatctcggg gaagtccggc tggtgaatcc ggtgctgcgc
      241 gtgtcgccgg agctgatggc cttgatgggc ggcggcggcg gcgcggcggg cgggaacggc
      301 tcggccatga cgctgcccgg ctggagcgtg gaccggctgg tttgtgatta cggtgagctg
      361 tatgtggcgg gcctgagcaa gcagccggtg aacgtcacgg cgcggttcgc gttcgattgg
      421 aaaaaccttt ccaccggcga gccgggggcc agccaggtca accgcctgca gctttggaac
      481 atcgccgcgt cgaagagcga ggattattcc acgccgtttc ttcggttcaa catcgtgaac
      541 ctcgacttca gcctgcggca attgctggag cggcggctgg ccttgatcga actcaacggc
      601 ggctttgtgc gcatcggcga ggaattccag gcgctgttga aggcgagttc cggcggcgct
      661 ccccaatccc ccgcccaggc ggcggcggtc tggattgcga atcaattgaa ggtcggggac
      721 attggcgtcc gggtggacga tccgcggccc gaagtggcgg acatccgctt tcggttgaac
      781 acggagctga ccgagtttcc gctgaacgat gtcgcggggg aaatcgcgca gcgcccgcag
      841 cggatcgaac tgagcaacgt gcagatcgta tcgcccgtgg acccggacac gcgcatcatc
      901 acgctgcgct cggtgttcct gaacttcacg ctcgccggct tgctggagca gcggattgat
      961 cggctgacgc tgctgcagcc gtcgattttc atctcgcagg atttgttcgt ttacatggat
     1021 cagatgaagg gcgccgatgc ggcggagccc ggccagaccg cgccgccgaa gccctcctgg
     1081 cgcatcaagg aattggaaat ccgctacggc cggctgctgc tcggcgggga aaagatcggg
     1141 cagatcgggc tgcccctcga gttcgaagcc agcgtgcgcg acatcctgct ggacaatctg
     1201 gcgtcgctcc agctccagac gcaattgacc atccggccgc gggattttga attcgccgcg
     1261 atgcagttga gcatggacca cctgcgcggc gacctgcggt tttcctatcc gcccgagcag
     1321 cggcaatcca acgtggtgaa cacccttttt ctcgacggca tccgctggcg gcaataccgg
     1381 ggccaaaagc tctggctctc ggtgaccttc gacaaacaga cgatctccgc gctcttcggc
     1441 ggcgaagcct acggcggcca gctgaacggc gggctggcct ttttcttttc gggaaacaac
     1501 cgctgggtgg gctggctgtc cgggcggggg gtggatctca agggcctcac ggacgtgatc
     1561 gcgccgcaga atttccggat gaccggaccg gcggatttca aggtgctcgt ggacgcgtcc
     1621 gggcagaaga tcgagcgctt gaagggagat tttacgggca cgcggaatgg caagatgact
     1681 ctctccaagc tcgacgattt cctggcgcgg attcctcccg agtggaacct gctcaagcaa
     1741 agctccacgc gcattgcgct ggaggccctg cgcgactacc gttacgaccg ggtgaaaggc
     1801 gatttttggt ttgtcaaccg gcagggagtg ctcaatctcc gaatggaagg cccgaccggg
     1861 aaacgcaact tcgaaatctt cctgcacacc gacgaatccg ctgaaggact atggcaaaaa
     1921 aaatccgcaa aatctggttc ttgatcccgc tcgcgctcgc ggcttgctcc gcgccatcgg
     1981 tcaatctcgc cacgccggaa ccgatcaagg tggacatcga catgcggctc gatgtctatc
     2041 agcacgccga tccgaacgcg gtcgcggttg ccgccgccgc cgccagcaaa cccaagccgg
     2101 cgccggccgc gaccccctcc agcgcccagg atccggcggt ccgccagaaa aatcgcgccg
     2161 cggagattca aaatttcaag aactcgcgct gggtgggcga ggggcgcgag ggtctgctgg
     2221 tcatccgcca gccgctgccc gacggcgacg cgggagacta catcgccaaa gtcgtccaac
     2281 aggaaaacgt ggaccgcaac cgcctgatgc gggagcaggc cgacgcgcag aaaaaaccgc
     2341 tggcggaaat ccaggcccag gccgccgcgg aatggcgcaa ccgttccttc tccggcgaat
     2401 tcatcgaagt ccagcagccg gacggcgcct ataagctcgt gcagaaggaa gggtagattt
     2461 tcttcatgag cgccatcgtt cccccgcgcc cgccatgctg aacgcgctgc gccggctttt
     2521 cctcggagcg gccctgctgc tggcggcatc ggcgggattg ctgctttccg atctggcttc
     2581 ccggacgggc gccgcctccg cggattcggc tccggaaaag accgcccggg tggccctgct
     2641 gcagcatgct tcccaggcca tcctggatca gggccgcgcg ggcatgctgg cgggattggc
     2701 cgaacgcggt tggacgccgg gccaaaatct ggaagtccgg aattacaacg cggaaggcga
     2761 catgccggtg gcgcaggcga tcgcccgcga aatggccaac ggcggccatc aactccttct
     2821 gaccatcagc accccctccc tccaggccgt ggccaatgcc aaccgcgcca cgcaaatccc
     2881 gcacgtgttt gccctggtga ccgatcccgt tgccgccggg gtgggcgtca atcgggagaa
     2941 tccgctggac catcccgcct ggctggcggg ttacggcacc atgcagcctg tggcgaaggc
     3001 gttcgcgacc gcgcgggaga tgaatccctc cctccgcacc gtgggcgtcg tctggaacgc
     3061 cgccgaggcc aattccgagg cccaggtaaa actcgcgcgg aaagtctgcg cggcgctgga
     3121 catccagctg ctcgaagcgt ccgtggaaaa caccgccggc gtcgccgaag ccgcggcttc
     3181 cctgggcgct cgcggcgcgg aggccctctg ggtgaacggt gacgtgaccg tcattaccgc
     3241 gattgattcg ctcctggccg ccgccaacaa ggcgaatatc cccgtcttca ccgtcatccc
     3301 gccgaacgtg aaatccggag ccctgttcga tctgggcgcg gattattttg aagtcggacg
     3361 gctgggcggg atcctggccg gggagatcct ctcgggcaaa gatcccgccg ccgtgccgat
     3421 tgaaaacgtg atgccggaaa tgctggcgct caacctgcgg accgcgggaa aattttcggc
     3481 gaaatggcgg attcccccgg cgcttcgcga acgcgcccgg ttgatcgtca acgaggacgg
     3541 cagcgagcag gccgccccgc cgccgaagac cgccgccgcg ccgaatccaa gcg
//
LOCUS       DATDAY010000109         3656 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_1419176, whole genome shotgun sequence.
ACCESSION   DATDAY010000109 DATDAY010000000
VERSION     DATDAY010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 3656)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3656)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3656
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1419176"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(110..805)
                     /locus_tag="VIS74_02090"
     CDS             complement(110..805)
                     /locus_tag="VIS74_02090"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HEY5792058.1"
                     /translation="MTAPFPSSIRFYSLLAACLLILPGCGILKDYQAYQANRAELKAF
                     KAMRKTWAAEHAAFRSQPGWKKRTYKNEALLKMASPENVRLEIALSEQRGLLLVSDSV
                     AMDFAVATGKRSHPTPAGSFAILSKSREYASNLYGKIYDATGVVTVADADTTRDTIPE
                     GGWFEGASMPYWMRLTNTGVGLHIGYVPGRPASHGCIRLPSAVAPVIFQRVRVGTPVV
                     IADTAPALGSGRN"
     gene            879..3332
                     /locus_tag="VIS74_02095"
     CDS             879..3332
                     /locus_tag="VIS74_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3656 domain-containing protein"
                     /protein_id="HEY5792059.1"
                     /translation="MPPSADVSRPELLAPAGDWDCLRAAVANGADAVFFGMPRFNARM
                     RADNFREEELPEVMRFLHAHGVKGYVTLNVLIFTGELPDAVAELALLNRCGVDAVIIQ
                     DVGLAEIARQMFPGLRVHASTQMTITSPEGVAFAGQLGVKQVVLARELSLREIKKFHS
                     PLPLEVFVHGALCVAYSGQCLTSESLGQRSANRGECAQACRMPYELVVDGALRDLGDK
                     RYLLSPQDLAAVREIPELIELGVKSFKIEGRLKSADYVAAVTQVYRKAIDAALNSEIP
                     NPKSEISPADWYKLEMTFSRGLFSGWMHGVNHQKLVSARFGKKRGPFAGRVTRVGRGW
                     VEVANEIPLRRGDGVVFDTGGDTNQEQGGRIYEIAGRRLSFEHGKMDFRKIAEGARVW
                     KTDDPVLEKELLASFQGRIPPREAGKRVVHFSLRGAAGESLEVTAECEARRVHCRSEI
                     PLQAALRQPLTAERAREQFAKLADTPFILGSLDFAVTDAVMLPMSELNRLRRETAAAL
                     ERRFIQPPSATLAEVMKSFRPAARSVAQTPELAVLCRSLEQIEPAVRAGADRVYLDFE
                     DIRRYREGVEAIRRLAATPVFLATPRIQKAGEQGFFKLIENAAPDGVLIRNIGAVPHF
                     AHSPLRKIGDFSLNVANPVTAFLFLRHGLEKLTVSYDLNADQVLDLLSEGPAGQLEIT
                     LHQHMPMFHMEHCVFAAFLSEGTDHTNCGRPCESHRVHLRDRVGMEHPLRADVGCRNT
                     LFNAVAQSGAAFYDSLRAAGAGSFRIELLEETPEASAEIVREYHRLLHGRISAGEVWR
                     RLKVRSQLGVTKGTLAAVE"
     gene            3383..3467
                     /locus_tag="VIS74_02100"
     tRNA            3383..3467
                     /locus_tag="VIS74_02100"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3417..3419,aa:Leu,seq:cag)
BASE COUNT          669 a         1149 c         1157 g          681 t
ORIGIN      
        1 gcgccgcgcc atcccgccgc gcctcgccga atggcgaggc aatcccgcga ggatgcgagc
       61 ttctctccca actaaaccgg atttccggat taacgggatt cccgattttt taattcctcc
      121 ccgaacccag cgccggagcc gtgtccgcga tcaccaccgg ggttcctacc ctcacgcgct
      181 gaaaaatcac cggagccacc gcgctgggca ggcggatgca gccatgcgag gccggccggc
      241 cgggcacgta gccgatatgc aggccgactc cggtgttcgt caaccgcatc caatacggca
      301 tcgaagcccc ttcaaaccag ccgccctcgg gaatcgtgtc ccgcgtcgtg tcggcatccg
      361 ccacagtgac aacccccgtc gcatcgtaaa tcttcccata taaattcgag gcgtattccc
      421 ggctcttgga gagaatggca aacgaaccgg ccggggtggg atgcgacctt ttgcccgtgg
      481 cgaccgcgaa atccatcgcc acggaatcgc tcaccaagag caggccgcgc tgttcggaga
      541 gcgcgatttc cagtctcacg ttttccggcg acgccatttt gagcagcgcc tcatttttat
      601 aagttcgctt cttccatcca ggctgggatc ggaaggccgc gtgctcggcc gcccaggttt
      661 tccgcatcgc cttgaacgct tttaactccg cccgattcgc ctggtaggcc tgataatcct
      721 tcaggattcc gcagccggga aggatcaaaa ggcaggcggc gagcagtgaa tagaaacgaa
      781 tcgatgaggg aaaaggtgcg gtcacgatca tcgggcaatt tagtggaggc ctcctgaatt
      841 cgcatggaaa aaaacggccg gaggggtaga gtttcttcat gccgccatcc gccgacgttt
      901 cccggcccga attgctcgct ccggcgggcg attgggattg cctgcgcgcg gccgtggcga
      961 atggcgccga cgccgttttt ttcgggatgc cccgctttaa tgcccgcatg cgcgccgaca
     1021 atttccgcga ggaggaattg cccgaagtca tgcggttcct ccacgcccac ggcgtcaagg
     1081 gctacgtcac gctgaacgtc ctcatcttca cgggcgaact tccggacgcc gtcgcggagc
     1141 ttgcgctgct caaccgctgc ggcgtggatg cggtcatcat tcaagacgtc ggcctcgccg
     1201 aaatcgcgcg gcagatgttt cccggcctgc gcgtccacgc gtccacgcag atgacgatca
     1261 cctcgcccga aggcgtggcc tttgccggcc agcttggcgt gaagcaggtc gtgctggcgc
     1321 gggaattgtc gctgcgggag atcaagaaat tccactcgcc gctgccgctg gaggtctttg
     1381 tccacggcgc attgtgcgtg gcgtattccg gccagtgcct gaccagcgaa tcgctcggcc
     1441 agcgcagcgc gaaccgcggc gaatgcgccc aggcctgccg gatgccgtat gaattggtcg
     1501 tggacggcgc gctgcgcgat ctcggcgaca agcgttacct gctctcgccg caggatctcg
     1561 ccgccgtccg ggaaattccc gaactcatcg agctcggggt gaagagtttc aagatcgaag
     1621 gccggctgaa atccgccgac tacgtggcgg cggtgaccca ggtgtatcgc aaggcgatcg
     1681 atgccgcgct gaattccgaa atccccaatc cgaaatccga aatctccccc gcggactggt
     1741 ataagctcga aatgacgttc tcgcgcgggt tgttctccgg ctggatgcac ggcgtgaacc
     1801 atcaaaagct cgtctccgcg cgtttcggca aaaagcgcgg tcccttcgcc ggacgcgtga
     1861 cgcgggtggg gcgcggctgg gtggaggtgg ccaacgaaat cccgctccgc cggggcgacg
     1921 gcgtggtctt cgacacgggc ggcgacacga atcaggaaca gggcgggcgc atttacgaaa
     1981 tcgccggacg gcggctttcc ttcgaacacg ggaaaatgga tttccggaaa atcgccgaag
     2041 gcgcgcgcgt gtggaaaacg gacgatcccg ttcttgaaaa ggaattgctg gcctcgttcc
     2101 agggccgcat cccgccgcgc gaggcgggca agcgggtcgt tcatttttcg ctgcgcggcg
     2161 ccgccgggga atcgctggag gtgaccgctg aatgcgaggc ccgccgggtt cactgccggt
     2221 cggaaattcc gttgcaggcc gccctgcgcc agccgctgac cgcggagcgg gcgcgcgaac
     2281 aattcgccaa gctggcggac accccgttca tcctcggctc gttggatttc gcggtgaccg
     2341 atgcggtgat gctgccaatg tccgaactca accggctgcg ccgcgagacc gctgccgcgc
     2401 tggagcggcg attcatccag ccgccgtccg ccacgctggc ggaagtcatg aagtcctttc
     2461 gcccggcggc ccgttcggtt gcgcaaactc cggaactcgc cgtgctctgc cgctcgcttg
     2521 agcagatcga gcccgcggtg cgggcgggag cggatcgcgt gtatctggac ttcgaggaca
     2581 tccgccgata ccgcgagggc gttgaagcga tccgccggct ggcggccacg ccggttttcc
     2641 tggccactcc ccggattcag aaggcgggcg agcagggatt tttcaagctc atcgaaaatg
     2701 ccgcgccgga tggcgtgctg atccgcaaca tcggagccgt gccgcatttt gcccacagcc
     2761 cgctgcggaa gatcggcgac ttttcgctga acgtcgcgaa tccggtgacc gcgtttttat
     2821 tcctccggca cggattggaa aaactgacgg tttcttatga tctcaacgcg gaccaagtgc
     2881 tcgacctgct gagcgagggc ccggccgggc agctggaaat cacgctgcac cagcacatgc
     2941 cgatgtttca catggagcac tgcgtgttcg ccgcgtttct gtccgagggc acggatcaca
     3001 cgaactgcgg ccggccctgc gaatcgcacc gcgtgcatct gcgggaccgc gtgggcatgg
     3061 aacatccgct gcgggccgat gtcggctgcc ggaacacgct ctttaacgcc gtcgcgcaaa
     3121 gcggcgcggc gttttacgat tcgctgcgcg ccgccggggc gggatcgttc cggatcgagc
     3181 tgctggagga aacgccggaa gcgagcgcgg agatcgtgcg ggaataccac cgcctcctcc
     3241 acgggcggat ttccgcgggc gaagtctggc gccggctgaa agtgcgctcc cagctgggag
     3301 tcaccaaagg cacgctggcg gcggtggaat agcggatttc gagtggacaa gcggcgaatt
     3361 tcacgccatc ttccacctcc ttgctcgtgt ggcgaaattg gcagacgcgt acgtctcaga
     3421 agcgtatggg gcaacccatg gaggttcgag tcctctcgcg agcaccattt attttcgatt
     3481 ggcgattttt gagtctggat tgacggatcg ctcttcacag cgcccggcga gagggctcga
     3541 acctcggttc gacggcaatg cgcggaagcg taaaaaactt tcccgcgaag ccgccgaacg
     3601 gcgctctggc cgattctctc atgccaaatg gatagttaga gcgattctca aaagag
//
LOCUS       DATDAY010000110         2591 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_1803442, whole genome shotgun sequence.
ACCESSION   DATDAY010000110 DATDAY010000000
VERSION     DATDAY010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2591)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2591)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2591
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1803442"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..269)
                     /locus_tag="VIS74_02105"
     CDS             complement(<1..269)
                     /locus_tag="VIS74_02105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792060.1"
                     /translation="MRDGNTKFSTRRPPAKDAARNEDRDRRLLVRESKVGQNVHPPLA
                     APEATRAPASVFSGRHSLPPVEADDKSWLRRFLFFIDEKKKAAKP"
     gene            310..>2591
                     /locus_tag="VIS74_02110"
     CDS             310..>2591
                     /locus_tag="VIS74_02110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792061.1"
                     /translation="MTSPIPFRFPFLPRFHSCRLAFLFATFAVLSGGHAQGDRIIADF
                     EAGTDGLTGGVARDPGAAKTGKASGRIDAVLANATSDPWVTVSKGLEFPNALTRLSFW
                     VKTRDADRLAVRITDATGQTFQLRPPLIRDGEWHEVAITTFEQGEGHQRFGGANDGKV
                     HWPAKSLHLILEKNYLSASGGNGTVWIDDLRVTLDPDRIVGNLDLVQDKLGNIFDSNE
                     PVRLFVDTQGDEVAWRVRDFWGNPLAEGVAPVKDKRAVIEPVKPGMGRLGYFEVNLDA
                     RKGGATLASTTTSYAVIAPADIHQMKDSPFGVMAHFAQGWDTDIMPLIAKAGVRVIRD
                     ELYWAEVEKKRGEYTFARYDPYMARAKELGLEPLIAMTFGNELYDHRSGPSTPAGYEG
                     YGNYGQAILKHYGPQIRWLEIWNEYNGSWSPPQANHDRPKYYTEMLKVASRKIKAVRP
                     DVKVLGGAAVLLPLPWFDGIFKNGGIEAMDGLVIHPYRWSPEGVDREIRDLRALAARY
                     GKKDMPVWVTETGSFDQTAEGRAIVARYLVKMCSLLLKEKSEMICWYLLRDYNEFKGM
                     GLLRDGNGPFGRYAPAPSYAAYANLIQRLHKAVPLGRDETPDALQVYRFDQGGIPVRV
                     AWALTPAEIAFEASSPVKVIDLMGGEQTLQPVGGRIHLTLDDEPVYLIGDVKPATGGP
                     LQLAAAQTIDYGGPFGLRYRLDGSASAEITFPGKAPVTLPPGQNQSVTLPGFDSARLG
                     NVRTRYTVTLGGKPVSTGVAL"
BASE COUNT          489 a          820 c          809 g          473 t
ORIGIN      
        1 catggcttcg ccgctttctt tttttcatcg atgaaaaaaa gaaagcggcg aagccatgat
       61 ttatcatcag cctcaactgg cgggagagaa tgccggcccg aaaataccga ggcgggagcc
      121 ctggtcgcct ccggcgccgc caaaggcggg tgaacatttt gcccgacttt tgattcgcgg
      181 acaagaagtc tccggtcacg atcttcattc cttgcggcgt cttttgccgg ggggcggcgg
      241 gtggagaatt tcgtattgcc atcacgcaat aatcttataa cattgcaaga tatctttctc
      301 agccctcccg tgacttcccc aatcccgttc cgctttccct tccttccccg tttccactcg
      361 tgccgcctgg cgtttttgtt cgcgacgttc gcggtcttat ccggggggca tgcccaaggc
      421 gaccgcatta tcgcggattt cgaggcggga acggatggcc tgaccggcgg ggtggcgcgt
      481 gaccccggcg cggcgaaaac cggcaaagcc tccggccgga tcgacgcggt tctggccaac
      541 gcgacctccg atccctgggt gaccgtctcg aaggggcttg aattcccgaa cgccctcacc
      601 cggctgagtt tctgggtgaa aacccgcgac gcggatcgtc tcgccgtgcg catcacggac
      661 gcgaccggcc agacgtttca gctccgtccg ccgctcatcc gggacggcga atggcatgaa
      721 gtagccatca ccaccttcga gcagggcgag gggcaccagc ggttcggcgg cgcgaacgac
      781 ggcaaggttc actggccggc aaagagcctc catctcatcc tggaaaagaa ttatctcagc
      841 gcctcgggcg gcaacggcac cgtgtggatc gacgacctcc gcgtgaccct cgatccggac
      901 cggatcgtgg ggaatctcga tctcgtgcag gataagctgg ggaatatttt tgattccaac
      961 gagccggtcc gcctgtttgt ggatacccag ggcgatgaag tggcctggag ggtgcgcgat
     1021 ttctggggga acccgctggc cgagggcgtc gcgccggtga aggacaaacg cgcggtcatt
     1081 gaacccgtca aaccgggaat gggccggctg ggctatttcg aagtgaatct cgatgcgcga
     1141 aaagggggcg ccacgctggc ttcgaccacc acctcgtatg cggtcatcgc tcccgccgac
     1201 atccatcaaa tgaaggattc tcccttcggc gtgatggccc atttcgcgca gggctgggac
     1261 accgacatca tgccgctgat cgccaaggcg ggcgtccggg tgattcgtga cgagctgtat
     1321 tgggccgagg tggaaaagaa gcgcggcgaa tacaccttcg cccgttacga cccctacatg
     1381 gcccgggcga aggaactcgg cctggagccc ctcattgcca tgactttcgg caacgaactc
     1441 tacgatcacc ggtccggccc cagcacgccg gcgggctacg agggttacgg aaattacggc
     1501 caggccattc tcaaacacta cggtccgcag attcgctggc tggaaatctg gaacgaatac
     1561 aacggcagct ggagtccgcc ccaggccaac cacgaccggc ccaaatatta caccgaaatg
     1621 ctcaaggtgg cgtcccggaa gatcaaggcg gtccgcccgg acgtgaaagt actgggcggc
     1681 gcggcggtgc tgctgccgct gccgtggttc gacggcattt tcaagaatgg cgggatcgaa
     1741 gccatggatg ggctggtcat tcatccctat cgctggagcc cggagggcgt ggaccgcgaa
     1801 atccgcgacc tgcgagccct ggccgcccgt tacggcaaga aggacatgcc ggtttgggtt
     1861 accgaaaccg gcagcttcga ccagacggcg gagggccgcg ccatcgtcgc ccgctatctg
     1921 gtgaagatgt gttcgctcct gctgaaggaa aaaagcgaaa tgatctgctg gtatctgctt
     1981 cgggactaca acgagttcaa gggcatgggg ctgctgcgcg acgggaacgg tccgtttggc
     2041 cgctatgcgc cggccccgtc ctacgcggcg tatgccaatc tcatccagcg cctgcacaaa
     2101 gccgtcccgc tgggccggga cgagacgccc gatgccctgc aggtttatcg cttcgaccag
     2161 ggcggaatcc cggttcgggt ggcctgggcg ttgaccccgg cggagatcgc cttcgaagcg
     2221 tcgtccccgg tgaaggtgat cgatctcatg ggcggcgagc aaaccttgca gcccgtgggg
     2281 ggccgcatcc atctcacgct cgacgacgag ccggtttatc ttatcgggga cgtgaagccg
     2341 gccacgggcg gaccgctcca actggccgcc gcgcagacca tcgattacgg cggaccattc
     2401 ggcttgcgct accgcctcga cggcagtgcc agcgcggaaa tcaccttccc cggcaaagcg
     2461 ccggtgaccc tcccgccggg ccaaaaccaa agcgtcacgc tccccggctt cgactccgcc
     2521 cggctcggca acgtgcgcac gcgctacacg gtgacccttg gcggcaagcc agtgtccacc
     2581 ggtgtcgccc t
//
LOCUS       DATDAY010000111         2616 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_1839584, whole genome shotgun sequence.
ACCESSION   DATDAY010000111 DATDAY010000000
VERSION     DATDAY010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2616)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2616)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2616
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1839584"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1641
                     /locus_tag="VIS74_02115"
     CDS             <1..1641
                     /locus_tag="VIS74_02115"
                     /EC_number="1.17.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HEY5792062.1"
                     /translation="IKAGLLRAGCDVPIVADIHFKPEAALEAAKWVEKVRINPGNYAD
                     SKKFAVREYTDSEYTAELGRIRERFAPLAALCKERGVALRIGTNHGSLSDRIMNRYGD
                     SPLGMVESALEFARIARELDYHHFIFSMKSSNPKVMIHAYRLLVARLNELDGEEMRNA
                     KCEGGNEAEIPHYQSWNYPIHLGVTEAGDGEDGRIKSAIGIGSLLSDGIGDTVRVSLT
                     EDSVYEIPVARALIQTAIPGAENSEIRNPKSEIPFDPFTYARRASERIEIQGTALGGE
                     ETIRVFTTQKKWDALAHKLDKLGDYRPELVLEQAGADAIDPRDDSAIEALNAAPAARL
                     VTVRDGLDLPVIAAYRLLAARVRPRHPILLKDTLGILDGGFRISDFLQTLLRAATPIG
                     ALLCEGIGDAVLVQGEEAPGQSLRLSYNILQAAGTRIFKTDYVACPSCGRTLFDLQST
                     TARIKASTQHLKGVKIAIMGCIVNGPGEMADADFGYVGGAPGKVNLYIGKTAVKFNIP
                     ETEAVDRLVDLIKEHHRWVEPAPAAAGSEMDLLALSAH"
     gene            1656..1889
                     /locus_tag="VIS74_02120"
     CDS             1656..1889
                     /locus_tag="VIS74_02120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792063.1"
                     /translation="MESFSLGERAQRALKIIASPQNFKVCEGCDSIVATRVATCPNCH
                     GYRFNEDAEAVSQQAQLLAGREQRTVTAQDLVN"
     gene            1947..2564
                     /locus_tag="VIS74_02125"
     CDS             1947..2564
                     /locus_tag="VIS74_02125"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HEY5792064.1"
                     /translation="MTPRLLRLALLALPLLGIGCASYDSRLTSTATQYLGANGAIVNG
                     PERKGYAESIAYWDGDGVSGAPSIVIDLSEQTSSFYKGGKLVGVSPISTGRSGYDTPA
                     GSFKILKKENREYRSNLYGDYVDDAGNVVVTNVGIKVDPMPPGTHFSGAPMPYFMRLT
                     NTGVGMHTGFLPGIPDSHGCIRMPDTMARTFSENVSVGTPVSIVP"
BASE COUNT          518 a          831 c          782 g          485 t
ORIGIN      
        1 atcaaagccg gcctgctccg ggccgggtgc gatgtgccga tcgtcgccga cattcatttc
       61 aagcccgagg ccgcgctgga agccgccaag tgggtggaga aggtccgcat caatccgggc
      121 aattacgccg attccaaaaa gttcgcggtc cgcgaataca ccgattcgga atataccgcc
      181 gagcttggcc gcatccgcga acgcttcgcc ccgctggccg ccctctgcaa ggagcgcggc
      241 gtcgccctgc gcatcggcac caaccacggt tcgctcagcg accgcatcat gaaccgctat
      301 ggcgattcgc cgctgggaat ggtggaaagc gccctggagt tcgcccgcat cgcgcgggag
      361 ctcgattacc accacttcat cttttcgatg aagtcctcga atccgaaggt catgatccac
      421 gcctaccggc tgctcgtggc gcggctcaat gaattggatg gcgaggaaat gcgaaatgcg
      481 aaatgcgaag ggggaaatga ggcggaaatt ccgcattatc aaagctggaa ctaccccatt
      541 catctcggcg tgaccgaagc cggcgacggc gaggatggcc ggatcaagag cgccatcggc
      601 atcggctcgc ttttgtcgga cggcatcggc gacacggtcc gcgtctcgct cacggaagac
      661 agcgtgtatg agattccagt ggcccgtgcg ctgatccaaa ccgccatccc cggcgcggaa
      721 aattccgaaa tccgaaatcc gaaatccgaa atccctttcg atcccttcac ctacgcgcgc
      781 cgcgcctcgg aacggatcga gattcaggga accgcgcttg gcggcgagga gacgatccgg
      841 gtttttacca cgcagaaaaa atgggacgcg ctggcccaca agctcgacaa actcggcgat
      901 taccgtccgg agctcgttct ggagcaggcc ggggcggacg ccattgatcc gcgggacgac
      961 tccgccatcg aagcgctgaa tgccgccccc gccgcgcgcc tggtcaccgt tcgcgacggc
     1021 ctcgatctgc cggtcatcgc cgcctaccgc ctgctggccg cgcgcgtgcg cccgcggcat
     1081 ccgatcctgc tcaaggacac gcttggaatt ttggatggcg gatttcggat ttcggatttt
     1141 ttgcaaacgc tgctgcgggc cgccacgccg atcggcgcgc tgctgtgcga gggcattggc
     1201 gatgccgttc ttgtccaggg cgaggaggct cccggccagt ccctgcgcct ttcctacaac
     1261 atcctgcagg cggccggcac ccgcattttc aagaccgatt acgtcgcctg cccgagctgc
     1321 ggccggacgt tgttcgacct gcaaagcacg accgcccgca tcaaggccag cacgcagcac
     1381 ctcaagggcg tgaaaattgc catcatgggc tgcatcgtca acggtcccgg cgaaatggcc
     1441 gacgcggatt ttggctacgt cggcggcgct cccggcaagg tgaatctcta catcggcaag
     1501 accgcggtga agttcaacat tcccgaaacc gaggcggtgg accggctggt ggatctgatc
     1561 aaggaacacc accgctgggt cgagcccgcg ccggcggcgg ccgggtcgga aatggattta
     1621 cttgcgttaa gcgctcacta ggggaaaatt aggtaatgga atcattttcc ctgggagagc
     1681 gtgcccagcg agcgctcaag atcatcgcca gcccgcagaa cttcaaggtc tgcgaaggtt
     1741 gcgactcgat cgtggccacc cgcgtggcga cctgcccgaa ttgccatggt taccgtttta
     1801 atgaggatgc cgaagcggtg agccagcagg cgcagttgct cgccggccgg gaacagcgca
     1861 cggtcacggc ccaggatttg gtgaattagc gcccccaagg gcaagcttga cccggaagat
     1921 tgaacactct atttgcatct cgatctatga ccccacgact gttgcgccta gccctgctgg
     1981 cccttcccct gttgggaatc ggctgcgcgt cttatgattc ccgcctcacc agcacggcca
     2041 cgcagtatct cggagcgaat ggggcgatcg tcaacggccc cgaacggaaa ggttatgcgg
     2101 aatccatcgc ttactgggat ggcgacgggg tgtccggagc cccgtccatc gttattgatc
     2161 tcagcgagca gacctcctcg ttttacaagg gcggcaaact cgtgggcgtt tcccccattt
     2221 ccaccggccg ctcgggatac gataccccgg ccggcagctt caaaatcctg aagaaggaaa
     2281 accgggaata ccgatccaac ctttacgggg actacgtgga tgacgccggc aatgtggtcg
     2341 tgaccaatgt cggcatcaaa gtcgatccga tgccgcccgg cacgcacttt agcggcgcgc
     2401 ccatgcccta ttttatgcgc ctgaccaata ccggggtcgg catgcacacc ggattcctcc
     2461 cgggcatccc cgactcgcac ggctgcatcc gcatgcccga tacgatggcc cggacttttt
     2521 ccgaaaatgt ctcggtcgga acgccggtaa gcatcgttcc gtagttttcg gaacgcctgt
     2581 agccgggatc gctgatcccg gccgatggca atttcg
//
LOCUS       DATDAY010000112         2393 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_225111, whole genome shotgun sequence.
ACCESSION   DATDAY010000112 DATDAY010000000
VERSION     DATDAY010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2393)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2393)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2393
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_225111"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..677)
                     /locus_tag="VIS74_02130"
     CDS             complement(<1..677)
                     /locus_tag="VIS74_02130"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEY5792065.1"
                     /translation="MSPRSKLKKLRFALAGTGAMANFHAQQLNAIPGVKIAAVCDVDE
                     ARVREFAAKYGVEEIYTDFDALLRQADFDALANVTPDAFHAPLTLKALAAKKHVLCEK
                     PLATHYADAKKMADAAKKAGVIHLVNFSYRKSGALQKAREFIQAGRIGRVVHVEGSYL
                     QSWLACDIWGPWRTTPAWLWRQSTRHGSKGVLGDVGVHLIDFASFPVGPIQKVHARLK
                     TFTGIKG"
     gene            complement(661..1344)
                     /locus_tag="VIS74_02135"
     CDS             complement(661..1344)
                     /locus_tag="VIS74_02135"
                     /inference="COORDINATES: protein motif:HMM:NF012774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HEY5792066.1"
                     /translation="MARRDAGGLIFGAFMRALACLWLLAAPLWAGEWQPLSDCRLRDS
                     PFNDGDSFHVTHGGKEYIFRLCYVDTPETRALRAYTERTTAQARYFGIKKSTLFKIAA
                     QASAFTRQTLNRPFTVWTDWTDARGNSQQPRFFGIIQTSGQDLAELLVARGFSRVYGY
                     SPDRPDGTPAAGYRAKLKQLEVRAFAEKAGAWAESKRKKPPKFSRSSRAHASPAAPSP
                     QATPHVPAI"
     gene            complement(1317..2294)
                     /locus_tag="VIS74_02140"
     CDS             complement(1317..2294)
                     /locus_tag="VIS74_02140"
                     /inference="COORDINATES: protein motif:HMM:NF016086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-E domain-containing protein"
                     /protein_id="HEY5792067.1"
                     /translation="MLSRVADSCFWLSRYIERAETNARLLDVNRQLLLDFEDQESEGS
                     RNYWTPILATLQDQEMFGKLYDRVDADSVLEYVTFEKKNPNSIVSCISAARENARTIR
                     EQISSEMWEQLNKVYLYLKSPAARSDYQQTPSDFYRSLMEGSHLFAGITDATMTHGEG
                     WLFLQAGKYLERADSTSRVLDIKYHLLLPRGEQVGGNVDITQWIAVLRSCSAREAYLK
                     IYEGQVTVAGVVEFLTLNASFPRSIRFSVDHVDRILHRISGQSTLSFTNDAERLSGLL
                     RSSIDYAQIGPILETGLHQYLDHVQQRLSEIAGALAKTYCEWLEETPAD"
BASE COUNT          421 a          670 c          834 g          468 t
ORIGIN      
        1 ttgcctttga tcccggtgaa ggtcttgagg cgggcgtgaa ccttctggat cggcccgacg
       61 ggaaaggagg cgaagtcaat gaggtggacg ccgacgtcgc cgagcacgcc cttgctgccg
      121 tgccgggtgg actggcgcca gagccaggcg ggggtggtgc gccacgggcc ccagatgtcg
      181 caggcgagcc agctttggag gtagctgccc tcgacgtgga ccacgcggcc gatccggccg
      241 gcctggatga attcgcgggc tttttgaagc gcgccggatt tgcgatagga aaaattcacc
      301 aggtgaatga cgcccgcctt tttggcggcg tcggccattt ttttggcgtc ggcgtaatgg
      361 gtcgccagcg gtttctcgca gagcacgtgc tttttggcgg ccagcgcctt gagcgtgagc
      421 ggggcgtgaa acgcgtccgg ggtgacattg gccagggcgt cgaagtcggc ctggcggagc
      481 agggcgtcaa agtcggtgta aatttcctcc acgccatatt tggcggcgaa ttcccggacc
      541 cgggcttcgt ccacatcgca aaccgcggcg atcttgacgc cgggaatggc gtttaattgc
      601 tgggcgtgga agttggccat ggcgccggtg ccggccaggg cgaagcgaag ttttttcagt
      661 ttagatcgcg gggacatggg gggtggcttg gggggaaggg gcggccgggg aggcgtgagc
      721 ccgggacgag cggctgaact tgggcggttt tttgcgtttg gattcggccc aggcccccgc
      781 tttttcggca aacgcgcgga cttcgagctg cttgagtttc gcgcggtaac ccgccgccgg
      841 cgtgccgtcc ggccggtcgg gcgaataccc gtaaacgcgg gaaaaaccgc gtgcgaccag
      901 cagctcggcg agatcctggc cggaggtttg aatgatgcca aaaaaccgcg gctgctggct
      961 gttgcccctg gcgtcggtcc aatccgtcca gaccgtgaag gggcggttca gggtttgccg
     1021 ggtgaaggcg gaggcctggg cggcgatttt aaaaagggtg gatttcttga ttccgaaata
     1081 ccgggcctgg gcggtggtgc gttcggtgta ggcccgaagc gcccgggttt ccggcgtgtc
     1141 cacgtaacaa agccggaaga tgtattcctt cccgccgtgg gtgacgtgga agctgtcacc
     1201 gtcgttgaaa gggctgtccc gaagccggca gtcggaaagc ggctgccatt ccccggccca
     1261 aaggggtgcg gcaagcagcc aaaggcaggc cagcgcgcgc atgaacgctc cgaaaatcaa
     1321 tccgccggcg tctcttcgag ccactcgcaa taggtcttcg ccagcgcccc ggcgatctcg
     1381 ctgagccgct gctgcacgtg gtcgaggtat tggtgcaggc cggtttcgag gatgggcccg
     1441 atctgcgcgt aatcaatgct ggaacgcagc aggccggaaa gccgctcggc gtcattggtg
     1501 aagctcagcg tgctctggcc ggagatgcgg tgcaggatgc gatccacgtg atccacgctg
     1561 aaacggatgg accgcgggaa ggaggcattc agggtgagga attcaaccac gccggcgacg
     1621 gtgacctgtc cctcgtaaat tttcagatac gcctcgcgcg cgctgcagga gcggagcacc
     1681 gcgatccact gggtgatgtc cacattgccg ccgacctgct cgccgcgggg gagcagcagg
     1741 tgatacttga tgtcgagcac gcgcgaagtg gagtcggcgc gttccaggta tttgccggcc
     1801 tggaggaaca gccagccctc gccgtgcgtc atggtcgcgt ccgtgatgcc ggcaaagagg
     1861 tgtgagcctt ccatgaggct gcggtaaaaa tcggagggag tttgctggta gtcgctgcgc
     1921 gccgccgggc ttttcaggta gagatagacc ttgttgagct gctcccacat ctcgctggag
     1981 atttgttcgc ggatggtccg cgcattttcg cgggcggcgg agatgcagga aacgatcgag
     2041 ttcgggttct tcttctcgaa ggtcacgtat tccaggaccg agtccgcgtc cacgcgatcg
     2101 tagagtttgc cgaacatttc ctgatcctgc agcgtggcca ggatcggggt ccagtagttg
     2161 cgggaaccct ccgactcctg gtcctcgaag tccaggagca gctggcggtt gacatccagc
     2221 aggcgggcgt tggtttcggc tcgttcgatg taacggctca gccaaaaaca ggaatcggcg
     2281 acacggctta acataaatta ttcttcgtcc agaacccagg tatccttgct tcccccgccc
     2341 tgcgaggaat tcaccaccag cgatcccttg cgcatggcga cacgggtgag ccc
//
LOCUS       DATDAY010000113         4462 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_2349252, whole genome shotgun sequence.
ACCESSION   DATDAY010000113 DATDAY010000000
VERSION     DATDAY010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 4462)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4462)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4462
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2349252"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..553)
                     /locus_tag="VIS74_02145"
     CDS             complement(<1..553)
                     /locus_tag="VIS74_02145"
                     /inference="COORDINATES: protein motif:HMM:NF013179.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEY5792068.1"
                     /translation="MAALLLIALAGFSLFLSPRAILIWTVVFSIPVTASVLGVADVLS
                     LPPGATFLRIASFFCAGGMAGLISAYRLHLSAEVGNFIKIFDALPVPLVVSDVDGEIQ
                     FANGACCELLRLPLEQVTASSYFSLFSDSQNRGREISRYLELFEDETSQSHVVLAIQL
                     AQGSIRRLATCFVVKIRAKPYLVT"
     gene            complement(881..2047)
                     /locus_tag="VIS74_02150"
     CDS             complement(881..2047)
                     /locus_tag="VIS74_02150"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEY5792069.1"
                     /translation="MRILQIFNRYLQFGGEESSVYQIGDALQSLHEVEYFLTSTESWI
                     TRRNGNRLAMLLDSFHNQYVLNRLRRYQTLGKFDIWQIHNVLPAMSSAVYAEAFRQGI
                     PIVHYLHNYRLSCVNGFFLNHGEPCMRCIQGNFFPAFQTACWRDSRWQSGAMGAVLMQ
                     MRSLQVFQKVARWVAISQRQKEIHVQMGLPEERIHVIPHFLECHVPPLPPSKEPVILF
                     VGRLSVEKGVFHLLNAWARLKTKSAQLLIVGDGPERPALEAQAAKLSLHGVSFRGFLS
                     PEQQTELWRRAVASVVPSIWEETFGKVVLEAWGRGRPVIGHRIGALPELIEPGVTGLL
                     AATGDAGALAGAMDQLLANPASSAEMGRQGHAMLLEKFSRERWLSQIRKIYETL"
     gene            complement(2051..3133)
                     /locus_tag="VIS74_02155"
     CDS             complement(2051..3133)
                     /locus_tag="VIS74_02155"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEY5792070.1"
                     /translation="MKSVLEVYLLPAYPAEGSHSMDNYWRALMEQAAVFERGGVSIQS
                     ALGIPPGQTRRRSRGERFMGKYLAYPLRAARIARGQRVHLLDHSFAHLLRFVPKSCPK
                     LVTVHDLVPLVDGRGLNGGQIARFRKTVECLRRADRLLAVSRFTAQALETVLSIPAAR
                     IQVIPMGVSVPVELPPRPAPDPSVFRLLSIGSGQARKNLDLLPEVIAPLIGRGRRLQL
                     VRIGSPLSGDLRERLGRLLEPQDLVERGEISVSAVKEEYRKAGALLFPSTLEGFGLPL
                     LEAMAQGCPVISSNASSLPEVGGDAVLYFSPADPLAAAAQVEKLILSPDLARSLIEAG
                     RQRARTFDWAVHAARLSEVYREMGGY"
     gene            complement(3130..4299)
                     /locus_tag="VIS74_02160"
     CDS             complement(3130..4299)
                     /locus_tag="VIS74_02160"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEY5792071.1"
                     /translation="MTILFLTKGYARYRRDLVEAVAEALSPRDTLLLATPSAKEELWE
                     VDWDSLSKNQGRVYYRDFPRAGLKDAFILLKRRVWPRPHRSEPAAARFRQLNPDLVVI
                     QEFSWPMLKVAICCRLAGIPCVVCSDIGEQTNWSYGQFSRRAWLVHALVSWIPTGLVA
                     HTPAAMNPIAAKRRPKIFIPHSIDLRRSRSALEHPQKTGDKVIGLVVAAYNERKGYDL
                     LAQAIRLLKEKGIRNMEFRWVGTENPAWLESVIATEGIGEGVKVLGVLKGEPLFQEFE
                     RADFFVLPTRADTYAVVVHEAAGFGLPLVISKFAGSASVLVNEGENGFVVDPNEIEVF
                     ADRLELLIGDEGLRQRFAQNSLRVGKKYCASQMGRQLAEWLLPHVRRDRSLLSSE"
BASE COUNT         1031 a         1416 c         1206 g          809 t
ORIGIN      
        1 gggtaaccag atagggtttg gcgcggattt tcaccacgaa acaggtcgcc aggcgccgga
       61 tgctcccttg cgccaattga atcgccagca ccacatggct ctgggaagtc tcgtcttcga
      121 acagctcgag gtatcgggaa atctcccgcc cgcggttctg ggaatcggaa aacaacgaaa
      181 agtagctgga tgcggttacc tgctccagcg gcagccgcaa caattcgcag caggcgccgt
      241 tggcaaattg aatctcgcca tccacgtccg aaaccaccag gggaaccggc agcgcgtcga
      301 agattttgat gaaattgccg acttccgcgg agaggtgtag gcggtaggcg ctgatcaagc
      361 cggccatgcc cccggcgcag aagaaagaag caatgcgcaa aaaagtggct cccggcggca
      421 gggaaagcac atccgccacg cccaggacgc tggccgttac gggaatgctg aaaacaacgg
      481 tccagatcaa aatcgcccgg gggcttaaga aaagcgaaaa acccgccagg gcgatcagca
      541 gcaatgcggc catgatccgg gcgctgggat tcagccactc gtgcagcaga accgccgcaa
      601 gaaccggaag cggagcaaaa ttgatccacc gcgaacggga tccggcccga tctgcaaaag
      661 gggagctcat ggacaaaacg ctgggaaata ctggctgggg agcacgggag ggatcatcac
      721 ctttagggaa ttatcaattt aatccgcaaa ttccatcgat tttcggttta attggacgcg
      781 ttcctctcgg cggcgttggc aacgccgctc caccggaagc tccgtctggc ggcagggcgg
      841 tgcggcaatg taaatggaaa acacgctaat cagcggattc tcagagcgtt tcataaattt
      901 tgcgaatttg cgacagccat cgctcgcggc tgaatttttc caacaacatc gcatggcctt
      961 gccgccccat ttccgcgctg ctggcggggt tggccagcag ctgatccatc gcgcccgcca
     1021 gcgcgccggc atctccggtg gccgccagca gccccgtgac acccggctcg atcaattccg
     1081 gcaatgcccc gatgcgatgc ccgatcaccg ggcggccacg cccccaagcc tccagcacca
     1141 ccttgccaaa agtctcctcc cagatggagg gcaccacgct cgccaccgcc cggcgccaga
     1201 gttccgtctg ctgctcgggg gaaagaaacc cccgaaaact caccccgtga agactcagct
     1261 tcgccgcctg ggcttccaat gccgggcgct ccgggccatc ccccacaatc aagagttggg
     1321 cggacttcgt tttcagccgg gcccaggcat tcaataaatg gaagaccccc ttttccaccg
     1381 agagccggcc cacgaagaga atcaccggtt ctttcgaggg cggcaatggc ggaacatggc
     1441 attccaaaaa atgcggaatg acatgaatcc gctcctcggg aagccccatc tggacatgaa
     1501 tttccttctg ccgctggctg atggcaaccc acctggccac cttctggaac acctggaggc
     1561 tgcgcatctg catgagcacc gcgcccatgg ccccgctctg ccagcggctg tcgcgccagc
     1621 aggcggtctg aaaggcgggg aaaaaattgc cctggatgca gcgcatgcag ggctcgccgt
     1681 gattcaggaa gaaaccgttc acgcaggaaa gccggtagtt gtgcagataa tgcacgatgg
     1741 ggattccctg gcggaaggcc tccgcataca ccgccgacga catggccggg agcacattgt
     1801 gaatctgcca gatgtcaaat ttgccaaggg tctgataacg acgcaaccgg ttcagcacat
     1861 attggttgtg aaaactgtcc aaaagcatcg ccagccggtt gccgttccgg cgcgtgatcc
     1921 agctttccgt cgaggtgaga aaatactcca cctcgtgcag gctctgcagc gcgtcgccga
     1981 tctggtaaac cgaactctcc tcgcccccga attgcaggta gcggttaaaa atttggagga
     2041 tgcgcatact tcaatatcct cccatttccc gataaacttc actgagccgg gcggcatgga
     2101 ccgcccagtc aaaggtccgc gctcgttggc gcccggcttc aatcaatgac cgggcgagat
     2161 cggggctcag gatgagtttc tcgacttgcg ccgccgccgc cagcggatca gcgggggaaa
     2221 aatagagcac cgcatccccc cccacttccg gcaacgagga ggcattactg gaaatcaccg
     2281 ggcagccctg cgccatagcc tcaagcaacg gcaggccaaa tccctccaag gtcgaaggaa
     2341 aaagcaaagc ccccgctttc cggtattcct ccttcacggc gctgacggaa atctcccccc
     2401 gctccaccag atcctgcggc tccaataatc gcccgagccg ttcgcgcaaa tcgccggaaa
     2461 gcgggctgcc aatcctcacc aattgcagcc gacggcctcg cccgataagc ggggcaatca
     2521 cctccggaag aaggtcgaga ttttttcggg cttgcccgct gccaatgctc aaaagccgaa
     2581 agaccgaagg atccggcgcc ggccggggcg gcagctcgac gggaacggaa acccccatgg
     2641 gaatcacctg gattcgggcc gcggggattg aaagaacggt ttccagggcc tgtgcggtaa
     2701 accgggacac cgccagcaag cgatccgccc gccgcaggca ctcaaccgtc tttcgaaagc
     2761 gcgcgatttg gcctccattg agcccgcgcc cgtccaccaa tggcaccaag tcatgaacgg
     2821 tcactaattt ggggcagctc ttcggaacaa accgcagcaa gtgcgcaaaa ctgtggtcta
     2881 acagatgcac cctctgtccg cgggcgattc gagccgcgcg caggggatag gcaaggtatt
     2941 tgcccataaa tctctccccg cgcgaacggc gccgggtctg gccaggagga atacccaaag
     3001 cactctgaat ggaaaccccg ccgcgctcaa atacggccgc ctgctccatg agggcacgcc
     3061 agtagttgtc catgctatgg cttccctccg ccggatacgc cggcaacaag tacacctcta
     3121 aaacggattt cattcggaac tcaaaagtga acgatccctt cgcacatgtg gcagcagcca
     3181 ctccgccagc tggcggccca tttgggaagc acagtatttt tttccgaccc tgagagagtt
     3241 ttgcgcaaac ctctggcgca acccttcatc gccgatcaaa agttccagcc tgtccgcaaa
     3301 tacttcgatc tcatttggat cgaccacaaa tccattttct ccctcgttca ccagcaccga
     3361 agcggaaccc gcaaatttgc tgatcaccaa aggcaatccg aaacccgccg cctcatgcac
     3421 gaccaccgcg taggtatccg cccgggtggg caggacaaaa aaatccgccc tctcgaactc
     3481 ctgaaacaag ggctccccct tcaggactcc caaaactttg actccctccc caatcccttc
     3541 ggttgcgatc accgactcca gccaggcggg attctcggtt cccacccaac ggaactccat
     3601 gtttcggatt cctttttctt taagaagccg gatcgcctgg gccaaaagat catagccctt
     3661 ccgctcatta taggcggcaa ccaccagtcc gatcacttta tctccggtct tttggggatg
     3721 ctccaaagcg gatcgcgacc tccgcagatc aatggaatgc ggtataaaga ttttcggacg
     3781 ccgcttggcc gcaatcggat tcatcgccgc gggggtgtgc gccaccaagc ccgtgggaat
     3841 ccacgaaacc agcgcatgca ccagccaggc cctgcgggaa aactggccgt aactccaatt
     3901 cgtttgttca ccaatgtccg agcagaccac gcagggaatc ccggctagcc ggcagcaaat
     3961 ggccactttc aacatgggcc aggaaaattc ctgaatgacc accaagtccg ggttcagttg
     4021 gcggaagcga gctgccgctg gttcgctgcg atgcgggcgg ggccaaaccc ggcgcttcag
     4081 gagaataaag gcgtccttca agcccgcccg gggaaaatcc cggtaataaa cccgcccctg
     4141 atttttcgaa agggaatccc aatccacctc ccaaagctcc tccttcgccg aaggcgtggc
     4201 caaaagaagc gtgtcacgcg ggctcaaggc ttccgcgacc gcctccacca agtcccgccg
     4261 ataccgggca taacccttgg taagaaacaa aatcgtcatc gcaaatatcc atttatcccc
     4321 ggacaactga aagggccggt gttttcattc ggcaataaaa ccagtttgaa ttgcgggggc
     4381 gggatgttca gattggtttt atggattccc gcttccttcg attcttcacc gccctcatcg
     4441 ggctggtcgt cgcgggttat ct
//
LOCUS       DATDAY010000114         4774 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_1250596, whole genome shotgun sequence.
ACCESSION   DATDAY010000114 DATDAY010000000
VERSION     DATDAY010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 4774)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4774)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4774
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1250596"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..452)
                     /locus_tag="VIS74_02165"
     CDS             complement(<1..452)
                     /locus_tag="VIS74_02165"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY5792072.1"
                     /translation="MPEPVRASRDLPVSVVIPTRNCAAAFERHWAALESWAPRCREIV
                     FVDSESSDGTLELVRKAAGSRANAVILSHPPGLYASWNAAIARCSADFTCIATVGDCV
                     APDGLAHLVETAERLGCDALLSPPGMRTGEGGPSAGRWPIHELAEARCP"
     gene            complement(445..1788)
                     /locus_tag="VIS74_02170"
     CDS             complement(445..1788)
                     /locus_tag="VIS74_02170"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY5792073.1"
                     /translation="MNESTPAPDANEPLIGVYGVGKTYRIWNASAARLRGTLMGAVGR
                     VFHPFGRIADSHQSKHSREFHALRGVTFEIQRGESVGIVGRNGSGKSTLLQIIAGTRL
                     PTHGHVSVRGRVAAILELGSGFRPDFTGRENVYINGAILGIPRSRIDEKFEEIAAFAD
                     IGDFIDQPVKTYSSGMMVRLVFAVQVALEPDIFIVDEALAVGDVFFQQKCAARMQELK
                     QRGTTLLFVSHSMQTVRSLCEKVLYLNDGQMQFFGSAIQGAQLYFSSSRPVHKAADKP
                     APSNEPPKEAADAFWRITESTAELAAAVTAVRVAPGQDLRAVRIGERAAFIVSVKSFT
                     EDELSLHVAFNLKNSFDRLVTAVGTHTTGSPPVMLAPWQSAEVRIEVELNVEAGDYTF
                     HFGVGLQEGTNGWQCYHSTPWMGPLTVSWPFDRETAPFYGPVGLPVSFLPLSGDA"
     gene            complement(1812..2663)
                     /locus_tag="VIS74_02175"
     CDS             complement(1812..2663)
                     /locus_tag="VIS74_02175"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY5792074.1"
                     /translation="MRKVFHRSYQLAADLIKSPVRCAVSLYGCRELLLHFIWRDFIGR
                     YRGAHFGALLSLASPIFMLAIYTIVFGYILNVRFSEEHTSKATFALALFSALTFFQLF
                     ADAISRAPVLIIENPNFVTKVIFPLEILPFSVVGTAFLHFVVSMGALLAGLLIFEHHL
                     SWSMAFAFLLVIPVLLYAAGCAWILAALGTFFRDIAALLPPLVLALTYLSAIFYPVSA
                     VPERLRPFLYANPFAVISEQAREIMIAGQPLGWVPWASVTLGGALVFMGGYLLFMRTK
                     RYFADSV"
     gene            complement(2685..4058)
                     /locus_tag="VIS74_02180"
     CDS             complement(2685..4058)
                     /locus_tag="VIS74_02180"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY5792075.1"
                     /translation="MRIAILDQSEPGWKAGEVFTCMMLASLDLADFGETEVVFLRRNP
                     DIIPPAKIAVSDIPWSPTREEWEAIQRRERLDAIVPVRDFSLEHILVAQVGWIPDFQH
                     VHLPEIFSAEELRARDRLFEEIARQCELVSFTCEAAAAEYRESHPAQAAKARVLRFPS
                     LMWRENLSADPARVARKFHLPAKFALAFNQMWKHKNHLVLPPAAAEVLRKHPDFYLVV
                     AGMPADYRDPQNRYLSAFLQACAECGVHDRIFLLGGISREDLVDLFRCAALIIQPSFH
                     EGWSTSVEDAKALGRPLACSELPVLREQAPHAAFFDPRHPASLAGALGPLWSAVRPGP
                     DLPNEARALAAARDDAQSYGESLLAVAEEAARIQRETLQTRMAAAKSALEGIFHPAAQ
                     GWWRIGSALALCARKPEAPPPLPESATLVESAGHYRMLEETRRALSLASRGLWFKLGR
                     ALRLVPR"
     gene            complement(4055..>4774)
                     /locus_tag="VIS74_02185"
     CDS             complement(4055..>4774)
                     /locus_tag="VIS74_02185"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEY5792076.1"
                     /translation="GNPVDMPRLMEIAARHRLTVIEDCAEAHGAEWEGRKVGSFGQLA
                     CFSFLSNKVIQCGEGGMVLTNDDRLAARMAYLRSLAFGPVRFFHEEPGYNFRLSAMQA
                     AMGVVQAGRIEEIVANKIDLFRRYRQRLEGVPWIQFPVEPRDGRHVHWMVGIRIRAGF
                     GITRDALRTSLTARGIDTRTLFCPMNQQPFLRAQPGYREVPCPVADRIWEDGLYLPST
                     TALTDGDLDEICAHVREAGNP"
BASE COUNT          954 a         1540 c         1549 g          731 t
ORIGIN      
        1 ggacagcggg cttccgccag ctcgtgaatg ggccagcgcc cggcgctcgg gccgccctcg
       61 ccggtccgca tgccgggcgg agaaagcaag gcgtcgcacc ccagccgttc ggccgtttcc
      121 acgaggtggg cgaggccgtc cggggccacg cagtctccca ccgtggcgat gcaggtaaaa
      181 tccgccgagc atcgggcgat ggcggcattc cacgacgcgt agaggcccgg cggatgcgag
      241 aggatgacgg cattcgcccg cgaacccgca gccttccgga cgagctccag ggtgccgtcc
      301 gaggattcgc tatcgacaaa aacgatttcc cggcagcgcg gcgcccagct ttccagcgcg
      361 gcccaatggc gctcgaatgc ggcggcgcaa ttgcgcgtgg gaatcaccac gctgaccgga
      421 agatcgcggg aagcgcggac gggttcaggc atcgccggaa aggggcaaaa aactgaccgg
      481 cagtcccacc gggccgtaga acggggcggt ttcccggtca aagggccagg agacggtcaa
      541 cggccccatc cagggcgtgg aatggtagca ttgccagcca ttggttcctt cctggagccc
      601 cactccgaag tggaacgtgt aatccccggc ttccacattg agttccacct cgatgcggac
      661 ctcggcgctc tgccaggggg cgagcatgac cggcggcgac cccgtggtgt gggtgcccac
      721 cgccgtgacg aggcggtcga aggagttctt gagattgaag gccacgtgca gggacaattc
      781 gtcttcggta aatgacttca cggaaacgat gaacgccgcc cgttcgccga tccgcaccgc
      841 gcgaagatcc tggccggggg cgacccgcac cgccgtgacg gcggcggcga gttcggcggt
      901 cgattccgtg atccgccaga aggcatcggc ggcttccttc ggcggctcat tcgaaggagc
      961 gggcttgtcc gcggccttgt ggacgggccg gctggaggaa aaatagagct gcgcgccctg
     1021 gatggccgag ccgaagaact gcatctggcc atcgttgaga taaagcactt tctcgcacag
     1081 gctgcgcacc gtttgcatgc tgtgggagac aaaaagcagg gtggtgccgc gctgcttgag
     1141 ttcctgcatg cgggcggcgc acttctgctg gaagaacaca tcgccgaccg ccagggcttc
     1201 atcgacgatg aaaatatccg gctccagcgc cacctggacc gcgaacacca ggcgcaccat
     1261 catgccgctg ctgtaggttt tcaccggctg gtcaataaaa tccccgatgt cggcaaacgc
     1321 cgcgatctcc tcgaatttct cgtcaatccg gctgcgcgga atgcccagga tcgcgccatt
     1381 gatatacaca ttttcccggc cggtgaaatc gggccggaag ccgcttccca gttccaggat
     1441 ggccgccacc cggccccgga cgctcacgtg gccatgcgtg ggcaggcggg tgccggcgat
     1501 gatctgcagc agcgtggatt tgcccgagcc attgcggccg acgatgccca cgctttcgcc
     1561 gcgctggatt tcgaaagtca cgccccgcag ggcatggaac tcccggctgt gtttgctctg
     1621 atgcgagtcc gcgatccggc cgaagggatg aaacacgcgg ccgaccgccc ccatcagcgt
     1681 gccgcgcagc cgcgcggcgg aggcgttcca gatgcggtag gtcttgccga cgccgtacac
     1741 gccgatcagg ggttcgttgg cgtcaggcgc cggggtgctc tcgttcatgc gatcgatggc
     1801 tccattcccg cttacaccga atccgcaaaa taacgcttgg ttctcataaa caaaagatac
     1861 ccgcccatga agaccagggc tccgccaagg gtcaccgaag cccacggcac ccagcccagg
     1921 ggctgtcccg cgatcatgat ttcccgcgcc tgctcggaaa tgaccgcgaa agggttcgcg
     1981 taaaggaagg ggcgcaggcg ctccggcacc gcgctgaccg ggtaaaaaat ggcgctcagg
     2041 taagtcaggg ccagcaccag gggcggcagc agcgcggcga tgtcccgaaa aaaggttccc
     2101 agcgccgcca gaatccaggc gcagcccgcc gcgtagagca ggacgggaat gaccagcagg
     2161 aaggcaaacg ccatgctcca ggaaaggtgg tgctcaaaga tcagcaatcc cgcgagcagg
     2221 gcgcccatgg acaccacgaa gtgcagaaag gcggtgccca ccacgctgaa gggaaggatc
     2281 tccagcggga agatcacctt cgtgacgaag ttcggatttt caatgatgag caccggcgcg
     2341 cgcgagatgg cgtcggcaaa gagctggaag aaagtcaggg cgctgaaaag cgccagcgcg
     2401 aaggtcgcct tgctcgtgtg ttcctcggaa aaccggacat tcaaaatgta tccgaagaca
     2461 atggtataaa tcgcgagcat gaaaatcggg ctggccagcg aaagcaacgc gccaaagtgc
     2521 gcgccccgat agcggccaat aaaatcccgc cagatgaagt gcaggagcaa ttcccggcag
     2581 ccgtagaggg aaacggcgca gcggaccggg gatttgatca aatccgcggc caattgatag
     2641 gaacgatgaa agacttttcg catgaatgga agagagagcc ggacttatcg cggcaccagc
     2701 cggagagccc ggcctaattt gaaccaaagg ccgcgggaag caagcgagag cgcgcgccgg
     2761 gtttcctcga gcatccggta atgcccggcc gactcgacga gcgtcgccga ttcgggaagc
     2821 ggcggcggag cctccggctt cctcgcgcaa agcgccagcg ccgagccgat ccgccaccag
     2881 ccctgcgcgg cgggatggaa aattccctcg agggcggatt tcgcggcagc cattcgggtt
     2941 tgcagggttt cccgctggat tcgcgccgcc tcctccgcga cggcgaggag ggattcccca
     3001 tagctttgcg catcatcgcg cgcggcggcc agcgcccggg cttcgttcgg caggtccgga
     3061 ccggggcgca ccgcgctcca cagcggcccg agcgcgccgg ccagggaagc cggatgccgc
     3121 ggatcgaaaa acgccgcgtg cggagcctgc tcccgcaaga ccggcagctc cgaacaggcg
     3181 agcggccgcc ccagcgcctt ggcgtcctcg accgaggtgc tccacccctc gtgaaacgag
     3241 ggctggatga tcagcgccgc gcagcggaac aaatcgacca gatcctcgcg gctgattcct
     3301 cccagcagga aaatgcggtc gtggacaccg cattccgcgc acgcctggag aaacgccgag
     3361 aggtagcggt tctggggatc gcggtagtcc gcgggcatgc cggcgacgac caggtaaaaa
     3421 tcgggatgtt tccgcagcac ctccgcggcc gccggcggca gcacgaggtg gttcttatgc
     3481 ttccacattt ggttgaacgc gagcgcgaac ttcgcgggca ggtggaactt gcgcgccacg
     3541 cgcgcgggat cggcgctgag attttcccgc cacatcagcg agggaaagcg cagcacccgc
     3601 gccttcgccg cctgggcggg atggctttcg cgatattcgg cggcggccgc ctcgcaggta
     3661 aacgacacga gctcgcactg ccgggcaatc tcctcaaaca aacggtcgcg cgcccgcaat
     3721 tcctccgccg aaaagatctc cggaaggtgg acgtgctgga aatcggggat ccagccgacc
     3781 tgggcgacca gaatgtgctc caggctaaaa tcgcggaccg gaacgatggc gtccaggcgc
     3841 tcccggcgct ggatcgcctc ccattcctcc cgggtcggcg accagggaat gtcggatacc
     3901 gcgattttgg cggggggaat gatgtccgga ttccggcgga gaaaaaccac ttcggtctcc
     3961 ccgaaatccg cgagatccag ggaggcaagc atcatgcacg tgaaaacctc cccggccttc
     4021 cagccgggtt cgctttgatc caaaatggcg attctcacgg attccccgcc tcccgaacgt
     4081 gggcgcaaat ttcatcgagg tcgccgtcgg tgagcgccgt ggtggagggc aaatacaggc
     4141 catcctccca aatgcggtcg gccaccggac agggcacttc gcggtaaccc ggctgcgccc
     4201 gcaggaaggg ttgctggttc atcgggcaga acagcgtccg ggtatcgatg ccccgggcgg
     4261 tcagcgacgt ccgcaacgca tcgcgggtaa tgccaaatcc cgcgcgaatc cggattccca
     4321 ccatccaatg cacgtggcgc ccgtcgcgcg gctcgaccgg aaactgaatc cagggcacgc
     4381 cctccaggcg ctgccggtag cgccggaaaa gatcgatctt gttcgccacg atttcttcga
     4441 tgcggccggc ctgcaccacg cccatggccg cctgcatggc cgaaagccgg aaattgtaac
     4501 cgggctcctc gtgaaaaaac cggaccggcc cgaaggcgag gctgcgcaag taggccatcc
     4561 gcgccgccag gcggtcgtcg ttcgtcagca ccatgccgcc ctcgccgcac tggatgacct
     4621 tgttcgacaa aaaactgaag caggccagct ggccgaaact gcccaccttt cggccctccc
     4681 actccgcccc gtgggcctcg gcgcagtctt cgatgacggt gaggcgatgc cgcgcggcga
     4741 tctccatcaa ccgcggcatg tccaccggat tgcc
//
LOCUS       DATDAY010000115         2415 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_2001118, whole genome shotgun sequence.
ACCESSION   DATDAY010000115 DATDAY010000000
VERSION     DATDAY010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2415)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2415)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2415
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2001118"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..516
                     /locus_tag="VIS74_02190"
     CDS             <1..516
                     /locus_tag="VIS74_02190"
                     /inference="COORDINATES: protein motif:HMM:NF012936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HEY5792077.1"
                     /translation="EFSPEEIYAEAIAAWDATPNAPLVDRATQYFVRLYLQDGILPKV
                     DRASMLHALEARSPFLDIELVDFVRRVPAKWKYHRGETKYLLKLALEPVLPREIIYRK
                     KKGFGTPIGTWFQQGRLAFDSPRLVPERLLESRLRAHRASVSDERQFLWNAWMLEAWG
                     KTRLSGRPAAA"
     gene            513..1628
                     /locus_tag="VIS74_02195"
     CDS             513..1628
                     /locus_tag="VIS74_02195"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEY5792078.1"
                     /translation="MKILTLCYEYPPLGGGGGRAAAAVAKALRERGHEVRLCTAALGG
                     RSFEENDGGVRVIRTGSFRRKADTCSVPEMGLYVATSFGPARRQIREWKPDVIHAHFA
                     VPTGALAWALQRLTGTPYVLTAHLGDVPGGVPEQTGALFRWIKPFTRPIWKNAAALTT
                     VSPFVAELAARAYGRLPEVIPNGIPLPPPPEPRRHQPPRILWVGRLSVQKNPRLALES
                     LAALGDLPWELHVVGDGPLRGELEDQSARLHLAERVTFHGWEDSPRVAELMRSSDLLL
                     MTSLSEGFPMVVVEALAHGLPVAATDIPALRGVILPGENGRLAAPPDLGAALRGLLKD
                     PETRARLAEGARASAARYDLREIARRYEAVLIKAAAR"
     gene            complement(1609..>2415)
                     /locus_tag="VIS74_02200"
     CDS             complement(1609..>2415)
                     /locus_tag="VIS74_02200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792079.1"
                     /translation="LLALAFMVCGLRQRRAADYSLGLGLLLGTGAALTLWPQYYFFRP
                     DLPHLSEFMVPFYVALGLMTYAAGAAMIRWRNPFVRAGLAALVLLALFDGIAYPYYAV
                     PKETAGGNAANRGRFSEFRGENGVNVRLKDWERDGIQALYEAVLKYSSPEDFITVYPY
                     SPTILLMTNRRSYEWNLYVDNATAPPDFFEKAIANIEWKRPAVIVIDNRDINKIEVSR
                     FQNWAKPLYDYIQAHYVDLGPFGRRSKLEVFVRPDKFQAVHANDVIAPRP"
BASE COUNT          424 a          856 c          745 g          390 t
ORIGIN      
        1 gaattttcgc cggaggaaat ttacgccgaa gccatcgccg cctgggacgc aactccgaac
       61 gcgccgctgg tggatcgcgc cacgcagtat tttgtgcgcc tgtatttgca ggatggcatt
      121 ctgcccaagg tggatcgcgc cagcatgctg cacgcgctcg aggcgcgctc gccttttttg
      181 gacatcgaac tcgtggactt cgtccgccgc gtgccggcca aatggaaata ccaccgcggc
      241 gagaccaaat acctgctcaa gctcgcactc gaacccgtgc tgccccgcga aatcatttac
      301 cggaaaaaga aggggttcgg cacgcccatc ggcacgtggt tccagcaggg ccgcctggct
      361 ttcgattccc cccgcctggt tcccgaacgc ctgctggaaa gccgcctccg cgcccaccgg
      421 gcaagcgtct ccgacgaacg gcagttcctc tggaatgcgt ggatgctgga ggcctggggc
      481 aaaacccggc tctccggccg gccagccgcc gcatgaaaat tctcacgctc tgctatgagt
      541 atccgccgct cggcggcggc ggcggccggg ccgccgccgc cgtggccaag gccctccgcg
      601 aacgcggaca cgaggtgcgc ctctgcaccg ccgcgctggg cggccgctcc ttcgaggaaa
      661 atgacggggg cgtccgggtg atccgcaccg gcagttttcg ccgcaaggcc gacacgtgct
      721 ccgtgccgga aatgggcctc tacgtggcga cgagtttcgg accggcgcgg cgccagatcc
      781 gggaatggaa gcccgacgtg attcacgcgc atttcgccgt tcccaccggg gcgctggcgt
      841 gggccctgca acgcctgacc ggaaccccct acgtgctcac cgcgcacctc ggcgacgtgc
      901 ccggcggcgt tcccgagcaa accggcgcgc tgttccgctg gatcaaaccc ttcacccggc
      961 cgatctggaa aaacgcggcc gcgctcacca ccgtgagccc cttcgttgcc gagctcgccg
     1021 cgcgggccta cgggcgcctg cccgaggtca ttcccaacgg catcccgctg ccgcctccgc
     1081 cggagccgcg ccgccatcag ccgccccgca ttctctgggt gggccggctg agcgtccaaa
     1141 aaaatccccg gctcgccctc gaaagcctcg ccgccctcgg cgatctgccc tgggaactgc
     1201 acgtggtggg cgacggcccc ctgcgcgggg aattggaaga ccaatccgcc cggctgcatc
     1261 tggcggaacg cgtgaccttt cacgggtggg aggactcccc ccgcgtcgcc gagctcatgc
     1321 gttcaagcga cctgctgctg atgacttcgc tcagcgaggg atttcccatg gtggtcgtcg
     1381 aagccctcgc gcacggcctg ccggtggccg ccaccgacat ccccgcgctg cggggagtga
     1441 ttttgcccgg cgaaaacggc cggctggccg cgccgccgga tctgggcgcc gcccttcgcg
     1501 gactgctcaa ggatccggaa acgcgcgccc gcctggccga aggcgcccgc gcttccgccg
     1561 cccgctacga tctgcgcgaa atcgcccgcc gctacgaagc cgtattgatc aaggccgcgg
     1621 cgcgatgacg tcgttcgcgt gaacggcctg gaatttgtcc ggccgcacaa aaacctccag
     1681 cttgctgcgc cgcccgaagg ggcccaaatc cacgtagtgc gcctggatgt aatcgtagag
     1741 cggcttggcc caattctgga aacgcgagac ctcgattttg ttgatgtcgc gattatcgat
     1801 gacgatcacc gccggccgct tccactcgat gttcgcgatc gccttctcga aaaaatccgg
     1861 cggcgccgtg gcgttgtcca cgtagagatt ccactcgtag gatcgccggt tcgtcatcaa
     1921 gagaatcgtc ggcgaatacg gatacacggt aatgaagtcc tccgggctgg aatatttcag
     1981 gaccgcctca taaagcgcct ggatgccgtc gcgttcccaa tccttgagcc gcacgttcac
     2041 gccattctcg ccgcggaatt ccgagaaccg cccgcggttt gccgcgttgc cgcccgccgt
     2101 ctccttcggc acggcataat agggatacgc gatgccgtcg aacagcgcca gcaggaccag
     2161 cgcggccagc ccggcccgga cgaacggatt gcgccagcga atcatggccg ccccggcggc
     2221 gtaggtcatc aggcccagcg cgacataaaa gggcaccatg aactcgctga gatgcggcag
     2281 atcgggccgg aaaaaataat actgcggcca gagagtcagc gcggcgcccg tccccagcaa
     2341 taatcccagg cccagcgaat aatccgccgc ccgccgctgg cgtaggccgc aaaccatgaa
     2401 cgccagagcc agcag
//
LOCUS       DATDAY010000116         3421 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_1383874, whole genome shotgun sequence.
ACCESSION   DATDAY010000116 DATDAY010000000
VERSION     DATDAY010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 3421)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3421)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3421
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1383874"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..579
                     /locus_tag="VIS74_02205"
     CDS             <1..579
                     /locus_tag="VIS74_02205"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEY5792080.1"
                     /translation="CDRLPAWVSGGNKSEKAEQAEAKGQLPAAVSLYENALDGTPATA
                     DIHRRLAVIYDSKLKDPISALHHYRRYLNLQAGEASPEIKSAVARLERELARKYAGAT
                     IGSSPKAANAPAPPPVDAKGFSTVPKTAAAEQLVGTETRTYKVQKGDTITAISRKFYK
                     TADRWKDIVDANFNQLNGKPDIREGQTLIIPE"
     gene            complement(916..1389)
                     /locus_tag="VIS74_02210"
     CDS             complement(916..1389)
                     /locus_tag="VIS74_02210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="R3H domain-containing nucleic acid-binding
                     protein"
                     /protein_id="HEY5792081.1"
                     /translation="MKQTAQEILDTLLGYLGFIVEIEEQERDGHPVLQIYTREAERLI
                     GHREQTLDDLQYLVNRILQAQDRDAPRVIVDVEHHRLIRDDLFIQKIRQMAESVRNTG
                     RPLQTEPLNSYDRRLVHNAFLNDPRITTWSPPDDSRIKRITLKPRATAGEAPSDA"
     gene            complement(1389..3122)
                     /gene="yidC"
                     /locus_tag="VIS74_02215"
     CDS             complement(1389..3122)
                     /gene="yidC"
                     /locus_tag="VIS74_02215"
                     /inference="COORDINATES: protein motif:HMM:TIGR03593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEY5792082.1"
                     /translation="MDRKTWLAVALSVIGLILWHFLYNVPEAERLNAQRAAHQAATVA
                     EAAAPPSATPTPALPAPEAPVMARRSQALTSEDSEYLFQNDTGGIEQVVLLKHEDEAT
                     GKVTLNADKDMPIGAYGLLPGQVLGGFEMSVDEKTNTVIFQKTEADGLLIEKRFSLPP
                     PDDKKGRYTVNLEVHFRNPTQAEIVRAGYFIATGGAASIHVNDLPTYTRFDWYRDGKM
                     SGIDVNWFNPGQIPLIGIQTSPAKLLYEQSTDQIAWAAVASQYFCTIVTAAPPEGAQV
                     WARRFNTYRKSGNTDIQGIQGGLGLKGFSLPAGQERVEKFQIYAGPKEYAVLSKLNPG
                     QKDVINFGFFGFVSEFLLWAMHGLHRALWQSYAAAIIALTILIKLCLWPLQNIATNSM
                     RRMAALAPKMTELREKYKDDPQKMNQETMKLYRDYGINPFSGCLPMLIQIPIFFGFYT
                     MLGTAVELRDSSFLWIQDLSQPDTIAHLFGFPINLLPLIMAVTMVWQMAITPKTGDAV
                     QQRIFYFMPVIFLVFCYNYASGLALYWTTQNIFSIVQLYMTRNKPLPVLEKKSVIAKR
                     EATLTKKKKRK"
     gene            complement(3126..>3421)
                     /gene="yidD"
                     /locus_tag="VIS74_02220"
     CDS             complement(3126..>3421)
                     /gene="yidD"
                     /locus_tag="VIS74_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEY5792083.1"
                     /translation="VLKFLLRLYQWTLSPLLAFIAGPGCGCRFEPRCSEYGIQALEAH
                     GPWRGSGLTLRRLLRCHPWGGFGCDPVPPATAPRMKAGEGFFHSKLETRNSKI"
BASE COUNT          663 a          974 c         1126 g          658 t
ORIGIN      
        1 tgcgaccgat tgcccgcctg ggtatccggc gggaacaaat cggaaaaagc cgaacaagcc
       61 gaggcgaaag gacagctgcc ggcggcggtg agcctttacg aaaacgcatt ggatggaacc
      121 ccggccacgg cggacattca ccggcggctg gcggtcattt acgattccaa gctgaaagac
      181 ccgatttccg cgctgcacca ttaccgtcgt tacctgaacc tgcaggccgg cgaggccagc
      241 ccggagatca aaagcgcggt ggcgcggctg gaacgcgaac tggcccgcaa gtatgccggc
      301 gccaccatcg gcagctcccc caaggccgcc aacgctccgg cgccgccccc cgtggatgcg
      361 aaggggtttt ccacggtgcc aaaaacggcg gcggcggagc aattggtcgg cacggaaacc
      421 cgcacttaca aggtgcagaa gggcgacacg atcaccgcga tctcgcgcaa gttttacaag
      481 acagcggacc gctggaagga catcgtggac gcgaacttca atcaactcaa cggcaagccg
      541 gacatccgcg aggggcagac gctgattatt ccggagtgag ccgcggcggc atccggagat
      601 tcgacggacg gctcggcgcc cgcccgcaac acttcgtgta gcgatgtggg cgggagccgt
      661 ccctgcctct cacgtggact tattagtatt tctcaaaaga gatttccgaa aagggatcaa
      721 tgcaggggca gccggagcga agaccgcctg tcccgcggaa gcgggacagc ccgaggggcg
      781 cacgaagtgc ggcaattgtg ccaaccgccc cgcgtaaacg gggatgcggg ggtccccgtt
      841 tccccgcaaa caaaggcggt tggcacaacc gcctcaaccc gtcgggtatt tcggaactcc
      901 cttttgagaa tcgctctaag cgtcggaagg cgcttcgccg gcggtggcgc ggggcttgag
      961 ggtgattcgc ttgatgcgcg aatcgtcggg cggcgaccag gtggtaattc gcgggtcgtt
     1021 gagaaaggcg ttgtgcacga gccggcggtc gtagctgttc agcggctcgg tctgcagcgg
     1081 gcggccggta ttccggacgg attccgccat ttgccggatt ttttggatga ataaatcgtc
     1141 gcggatcagg cggtggtgtt ccacgtccac aatgacgcgc ggcgcatcgc gatcctgcgc
     1201 ttggaggatg cggttgacga ggtattgaag gtcgtcgagc gtctgctcgc ggtgcccgat
     1261 gagccgttcg gcctcgcggg tgtaaatctg aagcacgggg tggccgtccc gttcctgctc
     1321 ctcgatttcg acgatgaagc cgaggtaacc cagcagggta tcgaggattt cctgggccgt
     1381 ttgcttcatt atttccgttt tttctttttc gtcagagtgg cttcccgttt ggcgatcacg
     1441 cttttcttct ccaggaccgg caagggttta ttgcgggtca tgtagagctg gacaatagag
     1501 aaaatattct gcgttgtcca atacagcgca aggccgctgg cgtagttgta gcagaagacc
     1561 aggaagatca ccggcatgaa atagaagatg cgctgctgca cggcatcgcc ggtcttcgga
     1621 gtgattgcca tctgccagac catcgtcacc gccatgatga gcggaaggag gttgatcggg
     1681 aaaccaaaca ggtgcgcgat ggtgtccggc tgcgagagat cctgaatcca caggaaggag
     1741 ctgtcgcgca gctccacggc ggttcccagc atcgtgtaaa agccgaaaaa gatcgggatc
     1801 tggatgagca tcggcaggca gcccgagaag ggattgatcc cgtagtcccg gtagagcttc
     1861 atcgtctcct ggttcatctt ctgcgggtcg tccttatatt tctcccgcag ttcggtcatc
     1921 ttcggcgcga gggccgccat ccgccgcatg gaattggtgg cgatgttctg cagcggccag
     1981 aggcagagct tgatgaggat cgtcagggca atgatggccg cggcataact ttgccacagc
     2041 gcgcggtgga ggccatgcat cgcccagagc aggaattcgc tgacaaagcc gaagaatccg
     2101 aaattgatga cgtccttttg gccgggattc agcttgctga ggaccgcgta ttccttcggc
     2161 ccggcgtaaa tctggaattt ttcgacgcgc tcctggccgg cgggcaggct gaatcccttc
     2221 aggccgaggc cgccctggat gccctggatg tcggtattgc cggacttgcg gtaggtatta
     2281 aaccggcgcg cccagacctg ggctccttcc ggcggcgcag ccgtgacgat ggtgcaaaaa
     2341 tactggctcg ccaccgcggc ccaggcgatc tggtcggtgc tctgttcgta gagcaatttg
     2401 gcagggctgg tctgaatgcc gataagcgga atctggccgg ggttgaacca attcacgtcg
     2461 attcccgaca ttttgccatc gcgataccag tcaaaccgcg tgtaagtcgg caggtcgttg
     2521 acgtgaatgg acgccgcgcc gccggtggcg atgaaataac ccgcgcggac gatctccgcc
     2581 tgggtggggt tgcggaaatg gacttccagg ttgacggtgt agcggccctt tttgtcgtcg
     2641 ggcggcggca gggaaaatcg cttttcgatc aggagcccat ccgcctcggt tttttggaaa
     2701 atgaccgtgt tcgtcttttc atccacggac atctcaaatc cgccaagcac ctggccgggc
     2761 aggagtccgt aggcgccgat cggcatgtcc ttgtcggcat tcagggtcac cttgccggtg
     2821 gcttcgtcct cgtgcttgag cagaaccacc tgctcgatgc cgccggtgtc attctggaaa
     2881 aggtattcgg aatcctcgct ggtcagcgcc tggctgcggc gcgccatgac gggagcttcc
     2941 ggcgccggga gggccggcgt gggggtggcg gacggcgggg ccgcggcttc ggccaccgtg
     3001 gcggcctggt gggcggcgcg ctgggcgttg aggcgttccg cctcggggac attgtaaaga
     3061 aagtgccaga ggatgagccc aatcacggag agggcgacgg cgagccaagt cttgcgatcc
     3121 attgattaaa ttttcgagtt tcgagtttcg agtttcgagt ggaaaaaacc ctcgccggct
     3181 ttcattcgcg gggcggttgc cggaggaacc gggtcgcaac caaagccccc ccagggatgg
     3241 cagcgcagca gccgccggag cgtcagcccg cttccccgcc agggtccgtg cgcttccagc
     3301 gcctggatgc catattccga gcaacggggc tcaaagcggc aaccgcaccc cggcccggcg
     3361 atgaaggcca gcaggggcga aagcgtccac tggtagagcc ggagcaggaa tttcaagacg
     3421 g
//
LOCUS       DATDAY010000117         4372 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_566755, whole genome shotgun sequence.
ACCESSION   DATDAY010000117 DATDAY010000000
VERSION     DATDAY010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 4372)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4372)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4372
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_566755"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..594
                     /locus_tag="VIS74_02225"
     CDS             <1..594
                     /locus_tag="VIS74_02225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792084.1"
                     /translation="GAIGQVGLIECAFAGHAPYDPESRLFANALGGGGLLDIGCYCVS
                     MARLVAGAAQGRDFAEPIRVAAMGRLDEREGTDLYTSALMEFPGAIFAALFTGIQLAH
                     ENQVRVFGSQGRITVTQPWLAGSKGARLIVRRRGGKPREIRTETKADLYGYEIDLVAK
                     HHAEKQAPPPAMTWEDSLGNARALDAWAKEIGLVYRF"
     gene            complement(794..1789)
                     /locus_tag="VIS74_02230"
     CDS             complement(794..1789)
                     /locus_tag="VIS74_02230"
                     /inference="COORDINATES: protein motif:HMM:NF013255.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEY5792085.1"
                     /translation="MKILLTGATGFVGRNLLLRLLREEKVARVIAAVRNPLKLRGQLK
                     HEGFAEIPAKLDILRAEAPEWEWDSREITHAIHSAGLISAARRDDYFRTNSHGTLRLL
                     ERLPPHLPTVILSSQAAAGPTPDGKPARAETDPAAPCTWYGESKWEMETRVLAQFPDR
                     PILFLRPPMVLGPRDTATLPLFRLARSPLRVKPGFAAKAFSWIGVGDLCAAIQQALAG
                     PFPSRAYFVASEGVVSDAQLIEAAGRAIRRRGIILPVPIPLLQGAAAVIGRHPGWSEK
                     IPNLTPDRVRDLLANRWVVDGSAFTRDFSWKPADQLAATLQTTAEWYRAQGLLAF"
     gene            complement(1786..2904)
                     /locus_tag="VIS74_02235"
     CDS             complement(1786..2904)
                     /locus_tag="VIS74_02235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792086.1"
                     /translation="MYSISVCQTRTDRSLFEKIPELLHGTDPSFTPPFPSSVAKFLEA
                     KSPYQRQHGKIQGFIARRNGVPCGRIAAITNRSHNAYHQDTTGFFGFFAAKNDPALAR
                     ALFAGAEAWLREQGCGRIRGPYNPSINDDCGLLTEGFEGIPFVAMPWNPSYYESLLDA
                     AEFKPIREMAAYMLDLSRGVPERIRKITARMRERSGWTIRGLRMNRLKEELATLYRLY
                     NCTLDRNWGFVPVTLEDFLASADDFRAIVDPEMILFAEKNGQAGAFAVAFPNFNEILL
                     QAKFTPPALRRLHIAWLMKTHRYHAQRLAVLGIAPGVRDQGLSAVLFAEQYERSARRY
                     QVSEVSWVEANNHEIQRGAEMMHGRKYRTYKIFERPLA"
     gene            complement(2795..4126)
                     /locus_tag="VIS74_02240"
     CDS             complement(2795..4126)
                     /locus_tag="VIS74_02240"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY5792087.1"
                     /translation="MAKDLTQKCREYRDSADARAQGFYPYFRRIDDTAGNHVLCDGAS
                     KIMIGSNNYLGLAQDERVVEAACAAVRRFGSGCTGSRLLNGNIQLHEQLEEALAEFLQ
                     RESVLLFSTGFFANQGALATLTEDDDAILCDRENHASIIDGCQFAPARLVPFRHNSAA
                     SLRNRLKRLPEAAGKLVVLDGVFSMSGDIADLPSILGVAKEFGALTYVDEAHALGVLG
                     PLGRGTAHHFGVNDDVDLVMGTFSKSLGSMGGFIAGSKEAIEYLRHKARCFIFTASLA
                     PAVAGGVLKALEIMQAEPERIDALWRNTRKMHEGFKQIGFKIGTTQTPIVPILIGSEA
                     QAFIFTQRLYEEGIFATPAIYPAVRYGEAIVRTSYMSSHTESDLDRVLSIFEKLAKEL
                     GTFDDPAYADPGRRKNVFDFRLPDSNGSQPVRENSGAASRDGSKLHATVSK"
BASE COUNT          787 a         1332 c         1446 g          807 t
ORIGIN      
        1 ggcgccatcg gccaggtcgg gctcatcgaa tgcgccttcg ccggccacgc gccctacgat
       61 cccgaaagcc gcttgtttgc gaatgcgctg ggcggcggcg ggctgctgga tatcggctgt
      121 tactgcgttt ccatggcgcg attggtcgcc ggagcggcgc agggccggga ttttgccgag
      181 cccatccgcg tggcggccat gggccggctg gacgagcggg agggaaccga tctctacacg
      241 agcgcgctga tggaattccc cggcgcaatc tttgcggccc tgttcaccgg aatccagctc
      301 gcccacgaaa atcaggttcg cgtgtttggc agccaggggc gcattacggt cacgcagccc
      361 tggctggccg gcagcaaggg agcccggctc atcgtgcgcc gccgcggcgg gaagccgcgg
      421 gaaattcgca cggaaacgaa ggcggacctt tacggctacg agatcgattt ggtggcaaaa
      481 catcatgccg aaaagcaggc gccccctccg gccatgacct gggaggacag cctgggaaac
      541 gcccgggcgc tcgacgcctg ggcgaaggaa atcgggctgg tttaccgttt ttaaaacctg
      601 tccggttgaa tgggacgcgt ttcccccggc ggggttggca accccgccgc tcgctgcgca
      661 atctgaatcg aaggggctct aaagcatttc tcaaaagaga tttccgaaaa gcgattcatt
      721 gtgaaggcgg ttggcacaag cgccactaca acgcgttggg tatttcggaa gcttctttta
      781 attaagaatc gctctaaaac gccagcagcc cctgggcgcg ataccattcg gccgtggttt
      841 gcagggtggc ggcaagctga tcggccggct tccaggaaaa gtcccgggtg aatgccgagc
      901 cgtccaccac ccagcggttc gcgagcagat cccggacgcg atccggggtc aggttcggaa
      961 ttttttccga ccagcccggg tggcgcccga tcaccgcggc ggctccctgc agcagcggga
     1021 ttggcacggg caggatgatc ccgcggcggc ggatggcccg gccggcggct tcgatcaact
     1081 gcgcgtccga gacgaccccc tccgaggcca caaaataagc ccgcgaagga aatggcccgg
     1141 ccagcgcctg ctgaatggcg gcgcagagat cgcccacgcc gatccagctg aaggccttgg
     1201 ccgcgaagcc gggcttgacg cggagcgggc tgcgggcgag gcggaaaagc ggaagcgtgg
     1261 cggtgtcgcg cgggcccagc accatgggcg gacgcaggaa cagaatcggg cggtccggaa
     1321 actgcgcgag cacccgcgtt tccatttccc atttcgattc gccataccac gtgcaggggg
     1381 cggcggggtc ggtttccgcg cgcgccggct tgccatccgg cgtcggcccc gcggcggcct
     1441 gggaggaaag aatcacggtc ggcaggtgcg gcggcaggcg ttcgagcagc cggagcgtgc
     1501 cgtgcgagtt ggtgcggaaa taatcgtccc gccgggccgc ggagatgagg ccggcgctgt
     1561 ggatcgcgtg ggtgatttcc cgcgaatccc attcccattc gggcgcctcg gcccgcagga
     1621 tgtccagctt cgcgggaatt tccgcgaagc cctcgtgctt gagctggccg cgcagcttga
     1681 gcggattgcg cacggcggcg atgacccgcg ccaccttttc ctcccggagc aggcgcagca
     1741 aaagatttcg gccgacgaag ccggtggcgc cggtcagcag gattttcatg ccagcgggcg
     1801 ttcgaagatt ttgtaggtcc ggtattttcg tccgtgcatc atttccgccc cgcgctggat
     1861 ttcgtggttg ttggcttcga cccaggaaac ttcggaaacc tggtagcgcc gggcggagcg
     1921 ctcgtattgt tcggcgaaca ggaccgcgct gagcccctgg tcccggactc ccggagcgat
     1981 gcccagaacg gccaggcgct gggcgtggta gcgatgcgtt ttcatgagcc aggcgatatg
     2041 cagccgccgc agcgcgggcg gcgtgaactt ggcctgaagc aggatttcat tgaagttcgg
     2101 aaaggccacg gcgaacgcgc cggcctgccc gtttttttcg gcgaagagaa tcatctccgg
     2161 atccacgatg gcgcgaaagt cgtccgcgga tgccaggaag tcctccagcg tcaccggaac
     2221 gaatccccag ttccggtcca gcgtgcaatt gtagaggcgg taaagcgtcg cgagttcctc
     2281 cttcagccgg ttcatgcgca gccctcggat ggtccagccg ctgcgttcgc gcatccgggc
     2341 ggtgattttc cggattcgtt ccggaacgcc ccggctcaga tcgagcatgt aggccgccat
     2401 ttcgcggatc ggcttgaatt ccgccgcgtc gagcagggat tcgtaatagg acggattcca
     2461 cggcatggcg acgaagggaa ttccctcgaa gccttccgtg agcagcccgc agtcatcgtt
     2521 gatgctggga ttgtacggcc cgcgaatgcg gccacagccc tgttcgcgca gccacgcttc
     2581 cgcgccggcg aaaagggcgc gcgcgagcgc gggatcattt ttggcggcga agaatccaaa
     2641 aaacccggtg gtgtcctggt gatacgcatt gtggctgcga ttcgtgatcg cggcgatgcg
     2701 cccgcagggc acgccgtttc ggcgggcgat gaagccctgg attttcccgt gctgccgctg
     2761 atacggactt ttcgcctcga gaaacttggc gacgctactt ggaaacggtg gcgtgaagct
     2821 tggatccgtc ccgtgaagca gctccggaat tttctcgaac aggctgcgat ccgttcgagt
     2881 ctggcagacg gaaatcgaat acatttttgc ggcggccggg atcggcgtaa gcgggatcat
     2941 cgaaggtgcc gagctccttg gcaagcttct cgaaaatgga gagcacgcgg tccaggtcgg
     3001 actccgtgtg ggagctcatg tagctggtcc ggacaatggc ctccccgtag cggaccgcgg
     3061 gataaatggc cggagtggcg aagatgcctt cctcgtagag ccgctgggtg aagatgaacg
     3121 cctgcgcctc gctgccgatg aggatcggca cgatcggcgt ctgcgtggtg ccgatcttga
     3181 aaccgatctg cttgaatccc tcgtgcatct tgcgcgtgtt gcgccacagc gcatcgatgc
     3241 gctcgggctc ggcctgcatg atctcgagcg ccttcaacac cccgccggcc acggcgggcg
     3301 caagcgaagc ggtgaaaatg aaacaccgcg ccttgtgccg cagatactcg atggcctctt
     3361 tcgagccggc gatgaagccg cccatcgagc cgagtgactt cgagaatgtt cccatcacga
     3421 gatcgacatc gtcgttcacg ccgaagtggt gcgccgtgcc gcggccgagc gggccgagca
     3481 cgccgagggc gtgcgcctcg tccacatagg tcagcgcgcc gaattccttc gccactccga
     3541 ggatggacgg caggtcggca atgtcgcccg acatgctgaa gacgccatcg agcacgacga
     3601 gcttgccggc cgcctcgggc aggcgcttga gacggttccg cagcgaggcg gcggaattgt
     3661 ggcggaaggg aaccagcctc gccggcgcga actggcagcc gtcgatgatg ctggcatggt
     3721 tctcacgatc gcagaggatc gcgtcgtcat cctcggtgag cgtggccagc gctccctggt
     3781 tcgcaaagaa gcccgtcgag aaaagcagga cggattcgcg ctggaggaat tcggcaagcg
     3841 cctcctcgag ctgctcgtgg agctggatgt tgccgttcag cagacgggag ccggtgcagc
     3901 cggagccgaa ccggcgcacg gcggcgcacg ccgcttcgac gacgcgttcg tcctgagcca
     3961 gaccgaggta attgttcgat ccgatcatga tcttcgaggc tccgtcacag aggacatgat
     4021 tgccggccgt gtcgtcgatg cggcgaaaat acggatagaa tccttgcgcc ctcgcatcgg
     4081 cggaatcccg atactcccgg catttctgtg ttaagtcttt cgccaccttg gatataatgc
     4141 cccgttcttt tgtccggatt tgtaaagtct tcgctcattt cgactccaaa cgccccgcgt
     4201 atcaatcaaa aaccggaggc aatgcttgcc tcaacgcggg gaatacccga ttatctgggg
     4261 ctgaccattt gtcaggtatc gttcgcgctg cctgcccgca tcgccgcttt cttttcgcat
     4321 cccattcctc tggaacgcag catggctcga taaggcgtcc gctcgagcgg ac
//
LOCUS       DATDAY010000118         2358 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_1602545, whole genome shotgun sequence.
ACCESSION   DATDAY010000118 DATDAY010000000
VERSION     DATDAY010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2358)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2358)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2358
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1602545"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..720)
                     /locus_tag="VIS74_02245"
     CDS             complement(<1..720)
                     /locus_tag="VIS74_02245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792088.1"
                     /translation="MTFLDRLERLLGHFAIPGLIRYVAALTGLVYLLGYASPDYLSYL
                     VLSPEKVMHGEVWRLISWIFVPGEGYGQWGPIWMAVGLMFLWFMGDIIEGAWGTFRLN
                     AFYFTGILACTLAAMLLPGAGTGLGANMFLNLSLILAVGTLVPDMQVLLFFIIPLKMK
                     WLAWFSVVLFALAFPVSPAATQAAMLISVGNYLLFFGPAFLRNRRRAQKTAVRRAQFE
                     QAAAPAETLHRCEVCGRTEVSN"
     gene            944..>2358
                     /locus_tag="VIS74_02250"
     CDS             944..>2358
                     /locus_tag="VIS74_02250"
                     /inference="COORDINATES: protein motif:HMM:NF018471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipase maturation factor family protein"
                     /protein_id="HEY5792089.1"
                     /translation="MIYDGDCRFCGKWIRRWKESTGEKVEYARSQDVGADFPEIPAER
                     YDAAVQWVEPGGQRAEGAEAVFRVLATANWRGRLLLWLFLKVGFFAWACRSSYAFVAR
                     HRAGFSLLTRWLWGESVERPTYAIASWLFLRALAVIYLIAFFSYWRQTDGLISSTGIL
                     PFQNFLLAARENLGMEAFWKAPTLGWLWQHDALPHLLCAGGVVLSVGLFFGWLPGPIL
                     ILLWAFYLSLTVMGQVFYNFQWDVLLLEAGFLAIFVAPWHRATWRHSGNPPSPARLLM
                     VWLLFRLIFSAGVVKFSSGDSTWAQLTALDFHFWTQPIPDWIAWYANLLPAPLKTAGV
                     VLMFGVELLLPLLFFTPRRLRFVAFSGTVLLQLLILATGNYGFFNLLTLALALWLMDD
                     LQWPGGLMSWVGSARVRYLPRRVAAGVALAIFVLTLAPFLGIFRQPMPLLRPLEWAYG
                     YIAPFRSINGYGLFAVMTTQRR"
BASE COUNT          410 a          686 c          784 g          478 t
ORIGIN      
        1 gttgctgacc tcggtgcggc cgcagacttc gcagcggtgc agggtctccg ccggcgccgc
       61 cgcctgttcg aactgggcgc gccggacggc ggttttttgc gcgcgccggc ggttgcggag
      121 aaaagccggc ccgaaaaaca acagataatt gccgacggag atgagcatcg ccgcctgggt
      181 ggcggccggg gaaacgggga aggccagggc gaagagaacc acgctgaacc acgcgagcca
      241 tttcatttta aggggaatga tgaaaaacag cagcacctgc atgtccggga ccagggtgcc
      301 gaccgccagg atgagcgata aattcagaaa catattcgcc cccaggccgg tgccggctcc
      361 gggcagcagc atggcggcca gcgtgcaggc gaggatgccg gtgaaataaa aggcgttgag
      421 ccggaaggtg ccccaggcgc cttcgatgat gtcgcccatg aaccacagga acatgaggcc
      481 gacagccatc cagatcgggc cccactggcc gtagccttca ccgggcacga agatccagga
      541 gatcagccgc cagacctctc catgcatcac cttctccggg ctgagcacga ggtaggaaag
      601 gtaatcgggc gaagcgtaac ccaaaaggta aacgaggccg gtcagggcgg cgacgtaacg
      661 gatgaggccg ggaatggcga aatggcccag gaggcgttca aggcggtcga gaaaagtcat
      721 gcgcgaagaa gctaaacgcc gcgcgaacgc atttcaactc cgggagaatg caaaccacgg
      781 atggacacag atgaacaccg atttaaggcg gaacagaaaa atttccaacc tcatccgtgt
      841 ttatcctgtc tatccgtggt tcaaacaatc gggaaactcc ttgactcgcc gcgggaagcg
      901 tttttgctcc atgcgagtgg ggaagaagcg cacacggcca attttgattt acgatggcga
      961 ctgccgtttt tgcggcaaat ggatccgccg ttggaaggaa agcaccggag agaaggtgga
     1021 atacgcccgt tcgcaggacg tgggcgccga ctttccggag atcccggccg agcgttacga
     1081 cgcggcggtg caatgggtcg aacccggggg gcagcgcgcc gagggagccg aggcggtctt
     1141 ccgcgtgctg gcgaccgcga actggcgcgg gcgcctcctg ctctggctgt ttttgaaggt
     1201 gggatttttt gcctgggcct gccggagcag ctatgccttc gtcgcgcgcc accgcgcggg
     1261 cttctccctc ctgacccgct ggctgtgggg cgagagcgtg gaacgtccga cctacgcgat
     1321 cgcttcctgg ctttttctgc gggcgctggc ggtcatttac ctgatcgcgt ttttttccta
     1381 ctggcggcag acggatggcc tgatcagctc caccggaatc ctgccttttc aaaacttcct
     1441 gctggcggcc cgggaaaatc tcggcatgga ggccttttgg aaggcgccca cgctgggctg
     1501 gctctggcag catgacgccc tgccgcatct gctgtgcgcg ggcggggtgg tgctatcggt
     1561 cgggctgttt ttcggctggc tgccggggcc gatcctgatc ctgctttggg cgttttatct
     1621 ttcgctcacg gtcatggggc aggtgttcta caatttccaa tgggatgtcc tgctgctgga
     1681 agccggcttt ctggcgatct tcgtcgctcc ctggcaccgc gcgacctggc ggcactcggg
     1741 gaatccgccc agccccgccc gcctcctgat ggtgtggctg ttgtttcgcc tgattttttc
     1801 ggcgggggtg gtaaagttca gttccgggga ttccacctgg gcgcagctca cggcgctgga
     1861 tttccatttt tggacgcagc cgattccgga ttggatcgcg tggtatgcga acctgctgcc
     1921 cgccccgctc aagaccgcgg gggtggtcct gatgtttggc gtcgagctgc tgctgccgct
     1981 gttatttttt acgccgcgcc gactgcgttt cgtggccttc agcgggacgg tgctgctgca
     2041 attgctgatc ctggcgacgg ggaattacgg cttctttaat ctcctgacgc tggcgctcgc
     2101 cctttggctg atggatgacc tgcagtggcc gggcgggctc atgagctggg tgggatcggc
     2161 gagggtgcgt tacctgccgc ggcgcgtggc cgccggagtg gcgctcgcca tttttgtgct
     2221 gaccctggcg ccgttcctgg ggatcttccg ccagccgatg ccgttgctca ggcccctgga
     2281 gtgggcttac ggatacatcg ctcccttccg ttcgatcaac ggctacggcc tctttgcggt
     2341 gatgaccacg caacggcg
//
LOCUS       DATDAY010000119         2423 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_1275580, whole genome shotgun sequence.
ACCESSION   DATDAY010000119 DATDAY010000000
VERSION     DATDAY010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2423)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2423)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2423
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1275580"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..299)
                     /locus_tag="VIS74_02255"
     CDS             complement(<1..299)
                     /locus_tag="VIS74_02255"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY5792090.1"
                     /translation="MARDFAAQGWRVAGCARSAEACRELREELGQLHDFQAVDVADDA
                     AVAEWAGRILARFGSPDLLINNAATIAPNAPLWEVARADFDRVIDVNVKGTVNVIR"
     gene            433..864
                     /gene="rplM"
                     /locus_tag="VIS74_02260"
     CDS             433..864
                     /gene="rplM"
                     /locus_tag="VIS74_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEY5792091.1"
                     /translation="MANSKTFSAKAETIDRKWWLIDAQDQLLGHVAVKAANLLRGKGK
                     TIFTPHIDSGDFVVVINADKVRIGGKKDVQKSYMSFSGYVGGHKSESFAARRERHPEL
                     IVERAVRGMIPHNRLGRSIYGKLKVYRGSEHPHTAQKIQNA"
     gene            869..1270
                     /gene="rpsI"
                     /locus_tag="VIS74_02265"
     CDS             869..1270
                     /gene="rpsI"
                     /locus_tag="VIS74_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEY5792092.1"
                     /translation="MSTATVNEYAATGRRKTAVARITLTPGKGNFEVNGKEFTEFFPS
                     LSLQNTILQPLEAVQSLHNYDFSATTAGGGITGQAGALRLAISRALLLVDETLRPILK
                     KEGFLTRDQRMKERKKSGQPGARKRFQFSKR"
     gene            complement(1381..2127)
                     /gene="cysA"
                     /locus_tag="VIS74_02270"
     CDS             complement(1381..2127)
                     /gene="cysA"
                     /locus_tag="VIS74_02270"
                     /EC_number="7.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter ATP-binding protein"
                     /protein_id="HEY5792093.1"
                     /translation="MSVEVTHVTKKFGKFAAVDDLSLKIETGELIALLGPSGSGKTTL
                     LRIIAGLEFPGEGHVIYAGEDVTEKSAYQRKAGFVFQHYALFHHLNVFENIAFGLRVR
                     PSRTRPKETEIRDRVLALLDKVQLKGLEKRLPSQLSGGQRQRVAFARALAIEPQVLLL
                     DEPFGALDAKVRKELRTWLRSFHDTTHLTSLFVTHDQDEAFEVADRVVVLHQGKIQQD
                     GTPREIRERPANDFVADFLGLYPESAASGI"
     gene            complement(2139..>2423)
                     /locus_tag="VIS74_02275"
     CDS             complement(2139..>2423)
                     /locus_tag="VIS74_02275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter permease CysW"
                     /protein_id="HEY5792094.1"
                     /translation="WRVTVPKIRWGLLYGVILCNARAMGEFGAVSVVSGHIRGQTNTM
                     PLQIEILYNEYQFASAFAVASLLSLLALLTLVLKTYAEWRAGRQLAAGTD"
BASE COUNT          507 a          789 c          685 g          442 t
ORIGIN      
        1 cggatcacat tcaccgttcc cttcacgttc acatcgataa cccggtcgaa atccgcgcgc
       61 gccacctccc aaagcggcgc attgggggca atggtcgcgg cgttattgat caacaaatcc
      121 ggcgagccga atcgcgccag aattcgcccg gcccattccg ccaccgcggc atcgtcggcg
      181 acgtccaccg cctgaaaatc gtgcagctgg cccaactcct cccgcagctc ccggcaggcc
      241 tccgccgagc gcgcgcagcc ggccacccgc cagccctgag cggcgaaatc ccgggccatt
      301 tcccggccca acccccgcga gcatccagtc aaaatgagaa tagattgtga ataacttccc
      361 atgcgctttt gaaggagagt agatttttaa ttgacactca agacgcgatc aatagaatta
      421 cccctccttt tcatggctaa ttcaaaaaca ttttccgcca aagccgaaac catcgaccgc
      481 aaatggtggc tcatcgacgc gcaggaccag ctcctcggcc acgttgccgt caaagccgcc
      541 aatctccttc gcggcaaagg caaaaccatt ttcacgcccc acattgatag cggcgacttc
      601 gtcgtggtca tcaatgccga caaggtccgc atcggcggca agaaggacgt tcagaagtcc
      661 tacatgagct tttccggcta cgttggcggc cacaagtccg aaagcttcgc cgcccgccgc
      721 gagcgccatc ccgagctgat cgtcgaacgc gccgttcgcg gaatgatccc gcacaaccgc
      781 ctgggccgct ccatttacgg caaactcaag gtgtatcgcg gcagcgaaca cccgcacacc
      841 gcgcaaaaga ttcagaacgc ttaatcccat gtccaccgca accgtcaacg aatacgccgc
      901 caccggccgc cgcaaaaccg cggtcgcccg catcaccttg actccgggca agggcaattt
      961 tgaagtcaac ggcaaggaat tcaccgaatt cttcccgagc ctgagcctgc agaacacgat
     1021 tcttcagccg ctcgaagccg tccagtcgct tcacaactac gatttctccg cgaccaccgc
     1081 gggaggcggc atcaccggcc aggccggcgc cttgcgcctc gccatttccc gtgcgttgct
     1141 gctcgtggac gaaaccctcc gcccgatcct gaaaaaggaa ggtttcctca cccgcgacca
     1201 gcgcatgaag gagcgcaaga agtcgggcca gcccggcgcc cgcaaacgct tccagttctc
     1261 gaagcgctaa tcggcccgga caatatttac gaaaaacggc gatcgaaaga tcgccgttgt
     1321 tttgtgcggc gcatcggcct catgagtccc ataaggccca tgggactcat gggagctctc
     1381 tcaaatcccg ctcgccgcgg actcgggata aagcccgagg aagtccgcca cgaagtcgtt
     1441 ggcgggccgt tcccggattt cccgcggcgt gccgtcctgc tggatcttgc cctgatgcag
     1501 gaccaccacg cggtcggcca cctcgaaggc ctcgtcctga tcgtgggtga cgaacaggct
     1561 ggtcaggtgc gtggtgtcgt gaaaactccg cagccaggtg cggagttcct tgcgcacctt
     1621 ggcgtcgagc gccccgaagg gttcatcgag cagcaggacc tgcggctcaa tcgccagcgc
     1681 gcgcgcaaag gccacgcgct gccgctggcc gccggagagt tgcgagggca ggcgtttttc
     1741 caatcccttg agttgcactt tgtccagcag ggccagaacg cggtcgcgga tttccgtttc
     1801 cttcgggcgc gtgcgggaag gccggacgcg caggccgaag gcgatgtttt caaacacatt
     1861 caggtgatga aacagcgcgt agtgctggaa cacgaacccc gccttgcgtt gataggcgct
     1921 tttctccgtg acatcttcgc cggcataaat gacgtgcccc tcgcctggaa attccaggcc
     1981 ggcaatgatg cgcagaagcg tggttttgcc cgagccgctg ggcccgagca gggcgatcaa
     2041 ttccccggtt tcgatcttta gggagagatc gtccaccgcg gcgaactttc cgaatttttt
     2101 ggtgacgtgg gtgacttcaa cactcatggc gtggatttct aatcggttcc ggcggccagc
     2161 tgccgtccgg cgcgccattc cgcataggtt ttcaaaacaa gggtgagcag ggccagcagc
     2221 gagagcagcg acgcgacggc aaacgcggag gcaaattggt attcgttgta gaggatttcg
     2281 atctggagcg gcatcgtgtt cgtctggccg cgaatgtggc cggagaccac ggacacggcg
     2341 ccaaattcgc ccatggcgcg ggcattgcag agaatcacgc cgtagagcag gccccagcgg
     2401 attttgggca cagtcacgcg cca
//
LOCUS       DATDAY010000120         4491 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_2331429, whole genome shotgun sequence.
ACCESSION   DATDAY010000120 DATDAY010000000
VERSION     DATDAY010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 4491)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4491)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4491
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2331429"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..237
                     /locus_tag="VIS74_02280"
     CDS             <1..237
                     /locus_tag="VIS74_02280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY5792095.1"
                     /translation="RAAGLTVYGGQNAPYIWVKGPEGQTSWQLFDRTLNEAHIVVTPG
                     SGFGGAGEGYFRISAFNSRANAEEAARRIQALPW"
     gene            complement(311..718)
                     /locus_tag="VIS74_02285"
     CDS             complement(311..718)
                     /locus_tag="VIS74_02285"
                     /inference="COORDINATES: protein motif:HMM:TIGR04256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HEY5792096.1"
                     /translation="MHPLYLKANVLSREVIGAAIEVHRLKGPGLIESIYEKCLSRELE
                     LRGINVIHQQSVEIEYKGFVFEETLRFDLLIEGCLLVELKSVQETLPIHKAQLLSYLK
                     LMDVPIGLLINFHQSKLTDGIHRMVLPDAATEN"
     gene            854..1780
                     /locus_tag="VIS74_02290"
     CDS             854..1780
                     /locus_tag="VIS74_02290"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HEY5792097.1"
                     /translation="MPKGPLLSEKEGMEPYLRELARIPALSDEEKRTIFAQTRAGNSE
                     ARERVVLAHLNLVVRTACQYAGYGLPLADLISEGNLALLRAAELFDPKHGVEFSTYAS
                     VWIKQRIHRAITAQARVVRIPVWRSQRLRKLGRLQDALSAELGREASSAELAEKLGLA
                     EDRLTRMAAEQVQVTELDETLRDDAFLEPGDRLSREELMEEIAACLSGLDDTELRIIS
                     LKFGLLDEESQSFREMAPRFGKSREWIRRVGEGALAKLRASFETIGTLPRSRIRRQKQ
                     ETARRLRQLQAGKLSLLHHALIYGLEPLIFTL"
     gene            1777..2472
                     /locus_tag="VIS74_02295"
     CDS             1777..2472
                     /locus_tag="VIS74_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HEY5792098.1"
                     /translation="MIAAILLFAGTMILSLWAAARVKSIYNRYSQLPAASGETGAEVA
                     AQILDRAGIRNVEIVAHNEMLGDHYDPIHKRLVLSEANYYGTSCSALGVAAHECGHAI
                     QDKIAFAPLRWRMAAVGTTTFANQIVLWLPLIGMFTGLIQPLTGALIMAIAWGVIMLF
                     NLITLPVEFDASTRAKQILAGMGHVATPEQMGAVRKVLDAAAWTYVAAFITSLAYLLW
                     HLLPLLMGRRDDE"
     gene            complement(2563..4173)
                     /locus_tag="VIS74_02300"
     CDS             complement(2563..4173)
                     /locus_tag="VIS74_02300"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter family protein"
                     /protein_id="HEY5792099.1"
                     /translation="MVDALLIAVAFLFGLLVRQFGLPPLVGFLMAGFVLQALGQTGGP
                     ALEALSNLGVTLMLFTIGLKLRLRSLLRPEIWGGTSLHTLGVVIGFGLVFVVLGVAGV
                     AGFNVWTALLLAFALSFSSTVFSIKSLEENGDMGSLHGRVAVGILVMQDVIAVLFLTV
                     STGKLPSWWAAALLASLILLRPAWSWLLARSGHGELIALCGLFLALVLGAKGFESVGL
                     KPDLGALFIGVLVGQHPKAKELGKSLLGLTDLLLVGFFLSIGLKGLPGPSGWLLAVLL
                     AVLLPLKSALFFGILTRFNLRARSSWIAGLNLGTYSEFGLIVLAPGVARGWVPAEWLV
                     IMAIAVSLSFLMAAPVCRRAEEFYDPISTWLKRFETPVHHPDDLPVQLQEERIAIFGM
                     GRVGVSAYNALQSRFPGRVIGFDRDPAQVEAHRAAERNVVLADATDSDFWERVSLRDH
                     MDLVVLAMPNHLANVHAAQTLKRHGYEGVVAATGKFDDEVKELRELGVDTAFNLYSEA
                     GAGFAHHVYNVFRQQRPDLVSSYRKEAN"
BASE COUNT          931 a         1444 c         1287 g          829 t
ORIGIN      
        1 cgggccgcgg gcctgaccgt ctatggcggc cagaatgcgc cttacatctg ggtgaagggg
       61 ccggaggggc agacgagctg gcagcttttc gaccgcaccc tgaacgaggc gcacatcgtg
      121 gtcactcctg gcagcggctt cggcggcgcg ggcgagggtt atttccgcat ctcggccttc
      181 aacagccgcg ccaacgcgga ggaagccgcc cggcgcattc aggcgctgcc ttggtgattt
      241 tttgaacaga aggacacaaa gagcacaaag atatttatct gatccctttg cgacctttgt
      301 gtccttctgt tcaattttca gtagccgcat caggcagcac catgcgatgg atgccatcag
      361 tcaacttgga ttgatggaag ttgatcaata atccgatcgg aacatccatc agtttcaggt
      421 agctcagaag ctgcgctttg tgaatcggca gggtttcctg gactgatttc aattcgacga
      481 gcagacagcc ttcaatcagc aggtcaaatc gcaaggtttc ttcaaagacg aagcccttat
      541 attcgatttc gaccgattgt tggtggatta cgttgatccc tctgagttca agttcacggg
      601 acaggcattt ttcgtaaatg ctctcgatca acccagggcc cttcaaacga tgcacctcga
      661 tggccgcgcc gatgacctcg cggctcaaaa cattcgcctt gagataaaga ggatgcacaa
      721 aagtttttga acagaagggc acaaagaaca caaagatatt tatctgatcc ctttgcggcc
      781 tttgtgtcct tctgttcaat tccccgttcc atttgtcact ccggcgggtt gtgctattca
      841 ttttaaaatc accatgccca aaggaccgct tttatccgaa aaggagggaa tggagccgta
      901 tctccgcgaa ctggctcgca tccccgcttt gtcggacgag gagaaacgga caatcttcgc
      961 ccagacgcgg gcgggaaatt ccgaggcgcg ggaacgcgtg gtgctcgcgc atttgaatct
     1021 ggtcgtccgc accgcctgcc aatacgccgg ctacgggctg ccgctggcgg atttgatttc
     1081 cgagggcaac ctcgcgctgc ttcgcgcggc ggaactgttc gatcccaaac acggcgtcga
     1141 gttttccacc tacgccagcg tctggatcaa acaacgcatc catcgcgcca tcaccgccca
     1201 ggcgcgcgtg gtccgcattc cggtctggcg ttcgcagcgg ctgcggaaat tgggccgcct
     1261 gcaggatgcg ctgagcgccg agctcggccg ggaagcctcc tccgcggagc tggccgagaa
     1321 actcggactc gccgaggacc gcctcacgcg gatggccgcc gagcaggtgc aagtcacgga
     1381 gctggacgag accttgaggg acgacgcatt cctcgaaccc ggcgaccgcc tttcgcgcga
     1441 ggagttgatg gaggaaatcg ccgcctgcct cagcggcctc gatgacacgg agctgcggat
     1501 tatttcgctg aaattcggac ttctggacga ggaaagccag tccttccgcg agatggcccc
     1561 gcgctttggc aaaagccggg aatggatccg ccgcgttggc gaaggcgcgc tggccaagct
     1621 ccgggcctcc tttgaaacga tcggcacgct tccccgcagc cggatccggc ggcaaaagca
     1681 ggaaaccgcc cggcggctgc gccagcttca ggccggcaag ttgtcacttc tccatcacgc
     1741 gctaatttat gggttggaac cattgatatt caccttatga ttgcagccat ccttcttttc
     1801 gccggcacca tgattttgtc cctgtgggcc gccgcgcggg tcaaatccat ttataaccgc
     1861 tacagccagc ttcccgccgc ctcgggcgag acgggcgccg aagtggcggc gcaaatcctg
     1921 gatcgcgccg gcattcggaa tgtcgaaatc gtcgcgcaca atgaaatgct cggcgaccat
     1981 tacgatccca ttcacaagcg cctggtgctt tccgaagcca attactacgg cacctcctgc
     2041 tccgcgctcg gcgtcgccgc ccacgaatgc ggccacgcca ttcaggacaa gatcgccttc
     2101 gccccgctgc gctggcgcat ggcggcggtg ggcaccacca ccttcgcgaa ccagatcgtg
     2161 ctctggctgc cgctcatcgg catgttcacc ggcctgattc agcccctgac cggcgcgttg
     2221 atcatggcca tcgcctgggg ggtcatcatg ctctttaatc tcatcaccct ccccgtggag
     2281 ttcgacgcct ccacccgcgc gaagcaaatc ctcgccggca tgggacatgt cgccacgccg
     2341 gaacaaatgg gcgccgtccg caaggtgctg gacgccgcgg cgtggaccta cgtggccgcc
     2401 ttcatcacct cgctcgccta cctgctctgg cacctgctgc cgctgctgat gggccggcgc
     2461 gacgacgaat agcggaaaac ggattgaaca gaaggaagca aaggcagcaa agaatggaat
     2521 taaccccttt gctatctttg cttccttctg ttcaaaaata cttcagttag cttctttccg
     2581 gtagctggag accagatccg ggcgctgctg gcggaagaca ttgtaaacgt ggtgcgcgaa
     2641 gcccgcgccg gcttcgctgt agagattaaa ggcagtgtcc acgccgagtt cgcgcagctc
     2701 cttcacctcg tcgtcaaact tgccggtggc ggcgaccacg ccttcgtagc catgccgctt
     2761 gagggtctgg gcggcgtgca cattcgccag gtggttcggc atggccagga ccacgagatc
     2821 catgtgatcg cgcaggctga cccgctccca aaagtccgaa tccgtcgcgt cggccagcac
     2881 gacgttgcgc tcggcggcgc gatgcgcctc cacctgcgcg ggatcgcggt cgaacccgat
     2941 cacccggccg ggaaaccggg actgcagcgc attgtaggcg gaaaccccca cccgccccat
     3001 gccgaaaatg gcgatgcgct cctcctgcaa ctgcaccggc agatcgtcgg gatggtgcac
     3061 cggggtctca aagcgcttca gccaggtgct gatgggatca taaaattcct ccgcccggcg
     3121 gcagaccggg gccgccatca ggaaactcag cgagacggca atcgccataa tcaccagcca
     3181 ttccgccggc acccatcccc tcgccacgcc gggggccagg acgatcaggc caaattcgct
     3241 gtaggtgccg agattgagtc cggcgatcca ggaactgcgc gcccggaggt tgaaccgggt
     3301 gaggatgcca aaaaagagcg ccgacttcag cggcagcagc accgcgagca acaccgccag
     3361 cagccagccc gagggtccgg gcaatccctt cagcccgatg ctcaggaaaa accccacgag
     3421 cagcaaatcc gtcagcccga gcagggattt gcccagctcc ttggccttgg ggtgctggcc
     3481 cacgagcacg ccgataaaga gggcgccgag gtccggcttc agcccgacgg actcaaaccc
     3541 cttcgcgccc aggaccaggg cgagaaacaa tccgcagagc gcgattaatt ccccgtgccc
     3601 ggaccgggcg agcagccagc tccacgcggg ccggagcaaa atgagcgagg ccagcagcgc
     3661 cgccgcccac cacgaaggca gcttgccggt ggagacggtt aggaacaaaa ccgcgatcac
     3721 gtcctgcatg acgaggattc ccaccgccac ccggccgtgg agcgagccca tgtcgccatt
     3781 ctcctccagg cttttgatgg aaaagaccgt gctggaaaaa ctgagcgcga acgccagcag
     3841 gagggcggtc cagacgttga aacccgccac tccggcaacc cccagcacca caaatacgag
     3901 cccgaatcca atcacgactc ccagcgtatg cagcgacgtt cctccccaaa tctcggggcg
     3961 caggaggctg cgaaggcgca gtttcagccc gatggtgaaa agcatgagcg tcactcccag
     4021 gttggacaag gcctccagcg cgggtccgcc cgtttgcccc agggcctgga gcacaaagcc
     4081 ggccatgaga aatcccacca acggcggcag cccgaactgc cggaccagca gtccgaacag
     4141 aaacgcgacg gctatgagta aagcatccac catttccctc cgggccattg ggattacgga
     4201 ccggcgcaaa aattagccag cccttttccg atttttcttc aatccggcgt ggctcaaaag
     4261 gttaacacag gccatttttc ccctcccaaa cgtcacaata ttgtcacatt ctagcggttg
     4321 aggccggggg atgcgcgggc agactttggc ctttcaaccc aaattcatca tcatgactac
     4381 ctctctacgt acaggcattg tcgcgttcag cgcgctggct ctggccaccg ccgcccaggc
     4441 ggaagtcgcc ctctccggag cgggcgcttc tttcccggct ccgctctacc a
//
LOCUS       DATDAY010000121         2912 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_1070491, whole genome shotgun sequence.
ACCESSION   DATDAY010000121 DATDAY010000000
VERSION     DATDAY010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2912)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2912)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2912
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1070491"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..761
                     /locus_tag="VIS74_02305"
     CDS             <1..761
                     /locus_tag="VIS74_02305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY5792100.1"
                     /translation="LPMFDHCQDYNIVGNGVMHEGEWSLRLGLPGWPRFGEEIIVARN
                     IQLAELTGTRIHCQHISSGGSIRLLREAKARGVPVSGEVCAHHVYFTDEALKGYDANF
                     KMNPPLREAGDIDRIVEGIADGTIEVLCSDHAPHAAYEKEVELDQAPFGILGLETELA
                     AFAEVLIHRRGVVGWPKLIELYTVNPAKLLKLPKGTLSEGADADVTLIDPDGEWTCDK
                     AESASLSRNNAFHGEKFRSRAVRTIVGGATVWSL"
     gene            794..1933
                     /gene="tgt"
                     /locus_tag="VIS74_02310"
     CDS             794..1933
                     /gene="tgt"
                     /locus_tag="VIS74_02310"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006983251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEY5792101.1"
                     /translation="MSFELLATDPSSKARAGLLTTRRGVIETPVFMPVGTQATVKAVS
                     PRELHELKAQIILGNTYHLHVRPGEKLIHELGGLHKFMGWDRPILTDSGGFQVFSLAR
                     IRKVTEEGVHFQNHLDGTPTFIGPEQSMEIQRNLDSDIVMAFDECPPHPSSHADAARS
                     LALTLRWAKRCREWWDAQPDASKPLVYGIVQGSSFADLRREAAEALVEMNFDSYAIGG
                     VSVGEPEPEMMLAVESAVPYLPENKSRYAMGLGTPPQMVEMIARGVDQFDCVLPTRLA
                     RNGTAFTAGGTIALKNAVYTRDDSPIEAGCECYACRNFSRAYLRHLIKAEEILGLRLV
                     SLHNLHFYLHLVRRCREHILAGTFAEFRKNFVPGYAVRKNGEEPA"
     gene            complement(1941..2297)
                     /locus_tag="VIS74_02315"
     CDS             complement(1941..2297)
                     /locus_tag="VIS74_02315"
                     /inference="COORDINATES: protein motif:HMM:NF018021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HEY5792102.1"
                     /translation="MNQMEPAAAEELARIRGELPPELNRALDKVSFIFERIPSPAEIA
                     EGIEPDQLGVFDGNALGETEFPMPTRIVLWLENLWDLAEGDADIFREEVRITLLHEIG
                     HFFGWGEDEMEARGLD"
     gene            2394..>2912
                     /locus_tag="VIS74_02320"
     CDS             2394..>2912
                     /locus_tag="VIS74_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEY5792103.1"
                     /translation="MSAATQPINPLREGLSNRAMPEPCTIVIFGATGDLTHRKLVPAL
                     YNLAANGSLPGSVSVVGFARRDKTDEIFRKELEEAAQKFSRQKLKPELWENFAANLFY
                     HRSEFDQAEGYEALAKRLDELDAQRGTRGNRLFYLSVAPDQFPIILEHLRKSGLNKAQ
                     PGSWARVIVEKPF"
BASE COUNT          571 a          901 c          912 g          528 t
ORIGIN      
        1 gcctgccgat gttcgatcac tgccaggact acaacattgt cgggaatggc gtgatgcacg
       61 agggcgagtg gagccttcgg ctggggctgc ccggctggcc gcggttcggg gaggaaatca
      121 tcgtggcgcg gaacatccag ctcgcggagc tgacgggcac gcggattcat tgccagcaca
      181 tcagttccgg cggaagcatc cgcctgctgc gcgaggccaa ggcgcgcggg gtgccggtct
      241 cgggcgaggt gtgcgcgcac cacgtttatt tcacggacga ggcgcttaag ggatatgacg
      301 caaacttcaa aatgaatccg ccgttgcgcg aggcggggga cattgaccgg atcgtggaag
      361 gcatcgcgga cgggacgatc gaggtgcttt gcagcgacca cgcgccgcac gcggcttacg
      421 aaaaggaagt ggagctggat caggcgccgt ttggaatcct cgggctcgaa accgaactgg
      481 ccgccttcgc cgaggtcttg attcaccggc gcggggtggt gggctggccg aagttgatcg
      541 aactttacac ggtgaatccg gcaaaactgc tcaagctgcc gaagggcacg ctctccgaag
      601 gcgcggatgc cgacgtgacg ctgatcgatc cggacggcga atggacctgc gacaaggccg
      661 aatcggcgtc gctgagccgg aacaacgcgt ttcatgggga gaagttccgg agccgggcgg
      721 tgcggacgat cgtgggtggg gcgacggttt ggagtttata ggtcccatcg gacttataag
      781 acccatggga cctatgtctt tcgagctgct ggcgaccgat ccgagtagta aagcgcgcgc
      841 ggggctcctg accacccggc ggggagtcat cgagactccg gtgttcatgc cggtgggcac
      901 ccaggcgacc gtcaaggcgg tgagcccgcg ggaactccac gaactgaaag cgcagatcat
      961 cctcggaaac acctaccacc tgcatgtgcg gccgggggaa aagctgatcc acgagctcgg
     1021 cggactgcac aagttcatgg ggtgggaccg tccgatcctg accgacagcg gcggattcca
     1081 ggtcttctcg ctcgcgcgga ttcgcaaggt gaccgaagaa ggcgtccatt ttcaaaatca
     1141 cctcgacggc acgccgacct tcatcgggcc ggagcagtcc atggaaatcc agcgcaatct
     1201 cgattcggac attgtcatgg cattcgatga atgcccgccg catcccagct cgcacgcgga
     1261 tgccgccagg agcctcgcgc tcacgctgcg ctgggcgaag cgctgccgcg aatggtggga
     1321 tgcccagccg gatgcctcca agccgctcgt ctatggcatc gtgcagggaa gcagcttcgc
     1381 cgacctgcgc cgcgaagccg cggaggcgct cgtggaaatg aacttcgaca gctacgccat
     1441 cggcggggta agcgtgggcg agccggagcc ggaaatgatg ctcgcggtcg aaagtgcggt
     1501 tccctatctg ccggaaaaca aatcccgcta cgccatgggg ctcggcaccc cgccgcagat
     1561 ggtggaaatg atcgcccgcg gagtggatca attcgactgc gtgctgccca cgcgcctggc
     1621 gcggaatgga accgcgttca ccgccggggg cacgatcgcg ctgaagaacg cggtttacac
     1681 ccgtgacgat tcgccgatcg aagccggctg cgaatgttac gcctgccgga atttttcgcg
     1741 cgcctacctg cggcatctca tcaaggccga ggaaatcctg gggctgcggc tcgtcagcct
     1801 gcacaacctc cacttctacc tccacctcgt ccgccgctgc cgcgaacaca ttctggcggg
     1861 caccttcgcg gaattccgca agaactttgt gccgggttac gccgttcgca aaaacggcga
     1921 agagccggcg tgaccggcgc ttaatccaac ccgcgcgctt ccatctcgtc ctcgccccag
     1981 ccgaagaagt ggccgatctc gtgcagcagc gtgatgcgga cctcttcgcg gaaaatatcc
     2041 gcatcccctt cggccaagtc ccacaggttt tccagccaga gcacgatgcg cgtcggcatg
     2101 ggaaattcgg tttccccgag cgcgttgccg tcgaacacgc ccaattgatc cggctcgatt
     2161 ccctcggcga tttccgccgg ggagggaatg cgctcgaaaa tgaaggacac cttgtccagc
     2221 gcgcgattga gctcgggcgg cagctcgccg cgaatccgcg ccagctcttc cgccgcggcc
     2281 ggttccattt gattcatcga aatcattaat attttgcaaa ttccagtggg tagtgcggct
     2341 tgccatcact tcccgccggg tttaagagtt cagctcactc gaaattactc aggatgtctg
     2401 ccgccactca acccatcaat ccgctgcgcg aaggtctttc caatcgcgcc atgcccgagc
     2461 cctgtaccat cgtcatcttt ggcgccaccg gagatctgac ccaccgcaag ctggttcccg
     2521 cgctctacaa tcttgccgcg aatggctcgc tgcctggctc cgtgagcgtg gtgggttttg
     2581 cccgccgcga caaaaccgac gagattttcc gcaaggaact cgaggaagcc gcgcagaaat
     2641 tctcccggca gaaattgaaa cccgagcttt gggaaaactt tgcggcaaat ctgttttatc
     2701 atcgttccga attcgaccag gccgagggtt acgaagcgct ggcgaagcgg ctggatgaac
     2761 tcgacgcgca gcgcggcacg cgcggcaacc ggctgtttta cctctcggtc gcgccggacc
     2821 agtttccgat catcctcgag catctccgca agagcggctt gaacaaggcg cagccgggca
     2881 gctgggcgcg cgtcatcgtc gaaaaaccct tc
//
LOCUS       DATDAY010000122         2319 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_2093284, whole genome shotgun sequence.
ACCESSION   DATDAY010000122 DATDAY010000000
VERSION     DATDAY010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2319)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2319)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2319
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2093284"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1067)
                     /gene="mdoH"
                     /locus_tag="VIS74_02325"
     CDS             complement(<1..1067)
                     /gene="mdoH"
                     /locus_tag="VIS74_02325"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF003958.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HEY5792104.1"
                     /translation="MTPAQPQKLTADFVAFRRASFFGAILATTAIGTWLMGRIFLPEG
                     ISVLEWIQLGLFALLFQQIATGFWLAFFGFAVTLLGGDRVRITRIQTTDKPPSPSAPT
                     AIVLPIYNEEVERVFRGVESMWRGLQAAGGTDGFDFFILSDSNRPESWLREEEAWVNL
                     CKRLHAFGRIFYRKRRAPRNGKSGNVADFCRRWGARYRYMIVLDADSVMTGRLLHHLV
                     AMMEQNPRTGLIQTSPQLAMGRTPFRRMQQFAARVYAPLFAAGSNYWHLFGANYWGHN
                     AIIRTQPFIEHCDLPELPEKETKHRHILSHDTVEAALMRKAGYDVWFAYDEEGSYEEG
                     PPNLSDSLARDRRWCQGNLQH"
     gene            complement(1064..1612)
                     /locus_tag="VIS74_02330"
     CDS             complement(1064..1612)
                     /locus_tag="VIS74_02330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792105.1"
                     /translation="MDAVNDAGWDEALSRLLAFLSALEMGGVEQRLRVALRIVDRARN
                     QAPADADPVRVTMSLFHEEMQQWFAQALEDPAISEDRRVALGVVALRLAPTQARWLDT
                     FPQNPPPAELRAALRGISLRTGPDLALSSMTPQEMDFGTMEDIAHETWHQFEWGPLLR
                     AVLIWTAIFFGALAAYNHFFPS"
     gene            complement(1600..>2319)
                     /locus_tag="VIS74_02335"
     CDS             complement(1600..>2319)
                     /locus_tag="VIS74_02335"
                     /inference="COORDINATES: protein motif:HMM:NF016252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein"
                     /protein_id="HEY5792106.1"
                     /translation="NTSNTFGDFRPEVHDSDGLLMQTGQGEWIWHPLAWSKQLQSNAF
                     KDDHPKGFGLMQRDRDFSHYQDLEAVYHKRPSAWIQPLSGFGQGEVRLIQLAARDEFM
                     DNIVSFWTPARPPEPLQPVEIEYVIRWLGDAADLSPLGRCLSTRQDYQEQPYYRHFFL
                     EFAGGDLGKLGEPPLVDVNSPTGAAINQIKIEKNDFNQSWRVSFETSTADNSKAVDLL
                     CRLVTKEGAPLTETWSYTWMP"
BASE COUNT          424 a          708 c          766 g          421 t
ORIGIN      
        1 aaatgctgca ggttgccctg gcaccagcgg cggtcgcgcg cgaggctgtc ggagagattc
       61 ggcggccctt cctcgtagct gccttcctcg tcgtaggcga accagacatc gtaaccggct
      121 ttccgcatca acgcggcctc cacggtgtca tggctgagaa tgtgccggtg cttggtttcc
      181 ttttccggca gttccgggag gtcgcagtgc tcgatgaagg gctgcgtgcg aatgatggca
      241 ttatgccccc agtaattcgc gccgaagagg tgccagtaat tcgaccccgc ggcgaagagc
      301 ggcgcgtaaa cccgcgcggc gaattgctgc atccgccgga agggcgtccg tcccatggcc
      361 agctgcgggg aggtctgaat caggcccgtg cgcgggtttt gctccatcat ggccacgaga
      421 tggtggagca ggcgtccggt catgacgctg tccgcatcga gcacgatcat gtagcggtag
      481 cgcgctcccc agcgccggca gaagtccgcg acgttgccgc tttttccgtt ccgcggcgcc
      541 cggcgtttgc ggtaaaaaat ccggccgaag gcatggaggc gcttgcagag attgacccac
      601 gcctcttcct cgcgcagcca gctttcgggg cggttcgagt cgctcaggat gaaaaaatcg
      661 aacccgtcgg ttccccccgc ggcctgcaac ccgcgccaca tgctttccac gccgcggaag
      721 acgcgttcga cttcctcatt gtagatcggg aggacaatgg ccgtcggggc tgaaggcgag
      781 ggcggcttgt cggtggtttg gatccgggtg attcggacgc ggtcgccgcc cagaagggtc
      841 acggcaaagc caaaaaaggc cagccagaat ccggtggcga tctgctggaa gagcagcgca
      901 aacaagccga gctgaatcca ttccagaacg gaaatgccct cgggcagaaa gatgcgcccc
      961 atcagccagg ttccgatggc cgtcgtcgcg agaatggctc caaaaaaact ggcgcggcgg
     1021 aacgccacga agtcagccgt caatttttgc ggttgggccg gggtcatgag ggaaagaaat
     1081 ggttataggc ggcgagcgcg ccgaaaaaaa tggcggtcca gatcagcacc gcgcgaagga
     1141 gtggccccca ctcgaattgg tgccaggttt cgtgggcgat gtcttccatg gtgccgaaat
     1201 ccatttcctg cggggtcatg ctggagagcg ccagatccgg gccggtgcgc agcgaaattc
     1261 cccggagagc cgcccgcagt tcggcgggcg ggggattttg cgggaaggtg tcgagccagc
     1321 gcgcctgggt cggcgccagc cgcagcgcca cgacgccgag ggccacgcgg cggtcttcgg
     1381 agatggcggg atcctccaat gcctgcgcga accattgctg catctcctcg tgaaagaggc
     1441 tcatcgtgac ccgcaccgga tcggcgtccg ccggggcctg gttacgcgcg cggtccacga
     1501 tccgcagcgc cacgcgcagc cgctgttcga ctccgcccat ttccaacgcc gaaagaaagg
     1561 cgagcaggcg gctgagcgcc tcgtcccatc ccgcatcatt cacggcatcc atgtgtaact
     1621 ccaggtttcg gtcaggggag ccccctcctt ggtcacgagg cggcagagca gatccacggc
     1681 cttggagttg tccgccgtgc tggtttcgaa gctgacgcgc caggactggt tgaagtcatt
     1741 tttttcgatc ttgatttggt tgatggccgc gccggtgggg gagttgacgt ccaccagcgg
     1801 cggttcgccg agcttgccga gatcgccgcc cgcgaattcg aggaagaagt ggcgatagta
     1861 gggctgctcc tggtaatcct gccgggtgga aaggcagcgt ccgagcggcg aaaggtccgc
     1921 cgcgtcgccc agccagcgga tcacgtattc gatctcgacc ggctgcagcg gttcgggcgg
     1981 ccgcgcgggc gtccagaaac tcacgatgtt gtccatgaac tcgtcccgcg ccgcgagctg
     2041 aatcaaacgg acctcgccct ggccgaatcc cgacaacggc tgaatccacg cgctgggccg
     2101 cttgtgatac acggcctcca agtcctgata gtggctgaaa tcccggtcgc gctgcatcag
     2161 cccgaagccc ttggggtgat cgtccttgaa cgcgttcgac tgaagctgct tcgaccaggc
     2221 gaggggatgc cagatccatt cgccttggcc ggtttgcatc agcaggccat cggaatcgtg
     2281 gacctcggga cggaagtcgc caaacgtgtt gctcgtatt
//
LOCUS       DATDAY010000123         3525 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_439403, whole genome shotgun sequence.
ACCESSION   DATDAY010000123 DATDAY010000000
VERSION     DATDAY010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 3525)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3525)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3525
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_439403"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..458
                     /locus_tag="VIS74_02340"
     CDS             <1..458
                     /locus_tag="VIS74_02340"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY5792107.1"
                     /translation="SKELMEPLRERIAGLKMGLEHRLDNAIGSLSGGQRQALTLLMAT
                     WLKPELLLLDEHTAALDPKSADQVISLTREVVSRDRLTTLMVTHSMQQAVSLGDRIVM
                     MNRGRVLHDFRGAEKKRLRAEDLMDRFEAVRRREQLDESAAAMLRQHYV"
     gene            522..1847
                     /gene="ffh"
                     /locus_tag="VIS74_02345"
     CDS             522..1847
                     /gene="ffh"
                     /locus_tag="VIS74_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEY5792108.1"
                     /translation="MFSSLSDKIQGVLKDLRGHGRISESNITPALREIRLGLLEADVH
                     FQVAKDFIARVKEKSLGEEVLHSVAPGQQIVKIFHDELTELLGGDAAPLKLDKPGRIL
                     IVGLNGAGKTTSSAKLAKLLKKDGKSPVLIACDLARPAAIDQLATLAGQIGVPVFRPE
                     PGEKDVIRTTRRALDWAAEQTGHNVQIFDSAGRQELDEALIQELVRLKEVVQPQEILL
                     VCDAATGQQAVSVAEKFHAALGLTGIILTKLDGDARGGAALSLRQVTQRPIKFAGTGE
                     KLDQFEAFFPERMAGRILGMGDVVSLVERAAEAITEEDAMRFERKFRTASFDLADFLE
                     QFKLLKKMGPLENILGMLPGMDKLKGSSVDESQLKRIEAIILSMTPGERAKPDMLNAR
                     RRQRVARGSGTTVTEVNNLLQRFNSMRKMMKKAGDFKKLAARARGSFKF"
     gene            1939..2196
                     /gene="rpsP"
                     /locus_tag="VIS74_02350"
     CDS             1939..2196
                     /gene="rpsP"
                     /locus_tag="VIS74_02350"
                     /inference="COORDINATES: protein motif:HMM:TIGR00002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEY5792109.1"
                     /translation="MAVAIRLRREGARNHPYYKIIVADQRSPRDGKFIEILGTYDPKR
                     EGENFSIALDRAEYWVGVGARPSQTVNSLINKARKKTAIAA"
     gene            2222..2452
                     /locus_tag="VIS74_02355"
     CDS             2222..2452
                     /locus_tag="VIS74_02355"
                     /inference="COORDINATES: protein motif:HMM:NF024483.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HEY5792110.1"
                     /translation="MEPFLRYVIGQLVEFPDDVVISETESEGMVRIHVAMGKSDLGRI
                     IGRGGHTIQALRTLLAAAGEKRGVKVFLNIQE"
     gene            2491..>3525
                     /locus_tag="VIS74_02360"
     CDS             2491..>3525
                     /locus_tag="VIS74_02360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792111.1"
                     /translation="MAKKILPLLAAVTVLLIGAGLVFFKFVVGPANPASFLPADTLFY
                     AAFPDFLQTAGRWNKTSLAKISQEPDVKKFLEKPLGKLLAAGKKDGSTVGRVLLDLKP
                     SRLFVSAQKLTEQEFQAVVGLQYWGGAASFQRALKELTAALAAGQPVESRTEKHGDFE
                     IEIFQLPQFQLAAARWKNWGFLANNPAALRAVLDSATQPGISKLAADPVYESTIRPLA
                     AQPDFLAFVRVEPVLDTVLAAGKKLGASAIPQQVNAIREAQAVGFSTKLAGADLQDVL
                     FIRTQKPLPLPPRLATRDIEFTNVSSIAFFEFALDWDRLKPDENLAPSPGLTADALAL
                     LDRLHAEARDA"
BASE COUNT          725 a         1071 c         1084 g          645 t
ORIGIN      
        1 tgtcgaagga attgatggaa cccttgcggg aacgaatcgc gggtttgaaa atggggctcg
       61 aacaccggct cgacaatgcc atcggctccc tttccggcgg ccagcgccag gcgttgactc
      121 tgctcatggc cacctggctg aagcccgaat tgctgctgct ggacgagcac accgccgcgc
      181 tcgatcccaa aagcgcggat caggtcattt cgcttacccg cgaggtggtg agccgggacc
      241 ggcttacgac cctcatggtg acccattcca tgcagcaggc ggtgagcctg ggggatcgca
      301 tcgtcatgat gaaccgcggc cgggtgctgc atgattttcg cggcgcggag aaaaaacggc
      361 tgcgggccga ggatctcatg gaccggttcg aggcggtgcg ccgccgggag cagctggatg
      421 agagcgccgc cgcgatgctg cggcaacatt acgtttgagg actccggccg cgggaagaaa
      481 tgagttgaac tggccggcaa aaacccgatg gtgattcgcg aatgttcagc tctctctccg
      541 ataaaatcca gggcgtcctc aaggatctgc gcggccatgg ccggatcagc gaatccaaca
      601 tcacgcccgc gctccgcgaa atccggctgg gattgctgga agcggatgtc cattttcagg
      661 tcgccaagga tttcatcgcg cgggtgaagg aaaagtcgct cggcgaggaa gtgctccaca
      721 gcgtggcgcc gggccagcag atcgtcaaaa tcttccacga cgaattgacc gagctcctcg
      781 gcggcgacgc cgccccgctc aaactcgaca agccgggccg catcctcatc gtcggcctca
      841 acggcgcggg taaaacgacc tcctccgcga agcttgcgaa acttttgaag aaggatggca
      901 aatcgccggt gctgatcgca tgcgacttgg cgcggccggc ggccatcgac cagctcgcca
      961 cgctcgcggg ccagatcggc gtgcccgtct tccggccgga gccgggggaa aaggacgtca
     1021 tccgcaccac gcggcgcgcg ctggattggg cggcggaaca gaccggccac aacgtgcaga
     1081 ttttcgactc cgcgggacgg caggagctgg atgaggcatt gatccaggaa ctcgtgcggc
     1141 tcaaggaagt cgtccagccg caggaaatcc tgctggtctg cgatgccgcc accggccagc
     1201 aggcggtgag cgtggcggaa aaattccacg ccgcccttgg cttgacgggc atcatcctga
     1261 ccaagctcga cggcgacgcc cgcggcggcg cggcgctttc cctgcgccag gtgacgcagc
     1321 ggcccatcaa attcgccgga acgggcgaaa aactcgatca attcgaggct tttttccccg
     1381 agcgaatggc cggacgcatc ctcggaatgg gggacgtcgt ttcactggtc gaacgggccg
     1441 cggaggcgat caccgaggag gacgccatgc ggttcgaacg caaattccgc acggccagct
     1501 tcgacctcgc cgactttctg gagcagttca agctgctcaa gaaaatgggg ccgctcgaaa
     1561 atatccttgg catgttgccg ggaatggata agctaaaagg ttcctcggtt gacgaaagcc
     1621 aactgaagcg catcgaggcg atcattctct cgatgactcc tggggagcgc gccaagccgg
     1681 atatgttgaa cgcgcggcgc cgccagcggg tcgcgcgcgg cagtggcacg acggtgaccg
     1741 aggtaaataa tctcctgcaa cggtttaact ccatgcggaa gatgatgaaa aaagcggggg
     1801 acttcaaaaa gctcgccgct cgagcaaggg ggagttttaa gttttaagtt tgttcgtttt
     1861 taacgaaaaa acgcaaactc gcttaaagct aacttttaca atcacttaaa ctttagactt
     1921 acaaacttaa aactcattat ggccgtagca atccgactcc gccgcgaagg cgctcgcaac
     1981 cacccgtatt acaaaatcat cgtggctgac cagcgcagcc cgcgggatgg caaattcatt
     2041 gaaatcctcg gcacctatga cccgaagcgg gaaggcgaga atttcagcat cgcccttgac
     2101 cgcgccgagt attgggtggg cgtgggcgcc cgtccttcgc agacggtcaa cagtctcatc
     2161 aataaggccc gcaaaaaaac cgccatcgcg gcctgagcgg cggctgcttc caccctgatc
     2221 cgtggaaccc ttccttcgct acgtcatcgg ccagctcgtg gagtttccgg atgatgtggt
     2281 catttccgaa accgaaagcg aagggatggt tcgcatccac gtggccatgg gcaaatccga
     2341 cctcggccgc atcattggcc ggggtggcca taccattcaa gccctgcgca cgctgctggc
     2401 cgccgccggc gaaaagcgcg gggtaaaggt ttttctgaac attcaggagt agcccggccg
     2461 ggcaatcccg gcgccgctct ccgaagtgaa atggccaaaa aaatccttcc cctcctcgcc
     2521 gcggtcaccg ttttgctgat cggtgcgggc ctggtgtttt tcaagttcgt tgtcggaccc
     2581 gccaatccgg cgtcctttct cccggcggac accctttttt atgcggcgtt tccggatttc
     2641 ctgcaaacgg cgggacgatg gaacaaaacc tcgctggcca agatcagcca ggagccggat
     2701 gtcaaaaagt tcctggaaaa gccgctggga aagctgcttg cggccgggaa gaaagacggc
     2761 tccaccgtgg gccgggtgct gctcgacctc aagccaagcc gcctgtttgt cagcgcgcaa
     2821 aagttgacgg agcaggaatt tcaagccgtg gtcggcctgc aatactgggg cggcgccgcc
     2881 agctttcagc gggcgttgaa ggagctgacc gcggcgctgg ccgcgggcca gccggtggaa
     2941 tcccgcacgg agaagcacgg cgacttcgaa atcgaaattt tccagcttcc gcaatttcag
     3001 ctggcggcgg cgcgttggaa aaattgggga tttctcgcga ataatccggc ggcgctccgg
     3061 gcggtgctgg attcggcaac gcagcccggc atttccaaac tcgcggccga tcccgtgtat
     3121 gagtccacga tccggccgct ggcggcgcag ccggattttc tcgcatttgt ccgggtggag
     3181 cccgtcctgg ataccgtcct cgcggccggc aaaaaactgg gcgcttccgc cattccccag
     3241 caggtaaacg ccattcgtga agcccaggcg gtcggatttt ccaccaaact tgccggcgcg
     3301 gatttgcagg atgtgctgtt tatccggacc caaaagccgc tgccgctgcc gccgcgcctg
     3361 gccacccggg acatcgaatt caccaatgtg tcctcgatcg cgttttttga attcgcgctg
     3421 gattgggacc ggctcaagcc ggacgaaaat ctcgcgccct cgcccggact caccgccgac
     3481 gcgctggcct tgctggaccg cctgcacgcc gaggcgcggg acgcc
//
LOCUS       DATDAY010000124         2300 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_775422, whole genome shotgun sequence.
ACCESSION   DATDAY010000124 DATDAY010000000
VERSION     DATDAY010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2300)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2300)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2300
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_775422"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..314)
                     /locus_tag="VIS74_02365"
     CDS             complement(<1..314)
                     /locus_tag="VIS74_02365"
                     /inference="COORDINATES: protein motif:HMM:NF013428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEY5792112.1"
                     /translation="MELPGIPKIASGKVREIFDLGNELLFVATDRLSAFDVVMPNPIP
                     GKGAVLTQLSNFWFARTQSIVPNHLTDRRPEDVVTSAADRAAVAGRSVVARRLKPLPI
                     EAV"
     gene            392..790
                     /locus_tag="VIS74_02370"
     CDS             392..790
                     /locus_tag="VIS74_02370"
                     /inference="COORDINATES: protein motif:HMM:NF027267.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0001558 - regulation of cell growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PrlF family
                     antitoxin"
                     /protein_id="HEY5792113.1"
                     /translation="MTSRQIWNCLAGVMDIHYLSGMAMVLEAEANLTAQNQITIPAAV
                     RKALNLQGGQSRVKFQILPEEGRVLVLRVEGGDAQEDPALEPFLNLLARDLRKHPKRI
                     IPFPAELVKKARLLVKGVDVNLDGPLTGAD"
     gene            844..1320
                     /locus_tag="VIS74_02375"
     CDS             844..1320
                     /locus_tag="VIS74_02375"
                     /inference="COORDINATES: protein motif:HMM:NF023093.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system YhaV family
                     toxin"
                     /protein_id="HEY5792114.1"
                     /translation="MIEVNGWKIYAHPLFIDQFEALIAEVEQLRAADPEGYRDKKKTK
                     LLAAVQKMAFEVIPRNPADRMFLQGNTLGPSYRHWHRGKFFEGRYRLFFRYSSTAKVI
                     VLAWVNDAETLRTYGSKADAYAVFKAMLARNRPPDEWGALLKESKEAASRTKRPPK"
     gene            complement(1342..>2300)
                     /locus_tag="VIS74_02380"
     CDS             complement(1342..>2300)
                     /locus_tag="VIS74_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY5792115.1"
                     /translation="KVRASGVDLGQVGRDLSAMVGGNYVNRFSIQGRSYKVIPQVKRT
                     ERLNPDQLDDIYVTGPDKQLVPLSTFATLKQTTQPRELKRFQQLNSAMIQGALYPGVP
                     LDLALKVLEAEAAKILPPGFVIDYGGQSRQLRVEGSKFLATMVLSVILIFLVLAAQFE
                     SFRDPLIILFGSVPLAISGALVFTVLGLTTLNIYSQVGLITLVGLVSKNGILIVEFAN
                     KLQAQGMAKLAAIIEAAATRLRPILMTSVAMIAGNIPLMIASGPGAGARNSIGIMLVS
                     GLIIGTAFTLFVVPAIYMLVAKDRSRARQKPQPPPEAAPTLA"
BASE COUNT          457 a          640 c          759 g          444 t
ORIGIN      
        1 accgcttcga tcggcagcgg cttgagtcgc ctggccacga cggaacgccc ggcgacggcg
       61 gcacgatcag cggcactggt cacgacgtcc tccggccgcc ggtccgtaag atggttcggg
      121 acgatcgact gcgtgcgcgc gaaccagaaa ttcgacagct gggtgagtac cgcgcccttt
      181 ccggggatag gattgggcat cacgacgtcg aaggccgaca gccggtcggt ggcgacaaac
      241 agcagttcat ttccgagatc gaaaatttcg cggaccttgc cgctggcgat tttcggaatt
      301 ccgggtaact ccaatgcaac caaatccata agggtgacgc taacgtgccg gacgggcgtt
      361 gccaagcaga aggagcctac gcgaaatccg catgaccagc cggcagatct ggaattgcct
      421 tgcaggggta atggatattc attacctttc tggcatggcg atggttttag aggcggaggc
      481 caatttgacc gcccagaatc aaatcacgat tccggcggcg gtgcggaagg cgctcaatct
      541 ccagggaggg caaagccggg ttaaattcca aatccttccc gaagaggggc gggttctggt
      601 tctgcgggtg gaagggggcg atgcgcaaga ggatcccgct ctggagccat ttttgaactt
      661 gctggcacgg gatctgcgca agcatccgaa gcggatcata cccttcccgg ccgagttggt
      721 taagaaggct cgtttgctgg tcaagggcgt cgatgttaat ctcgacggtc cgcttaccgg
      781 cgcggattag agccgcttgt gccttcccct cgccgcccca aagcctcgga ttccgcgcct
      841 cctttgatcg aggtaaacgg atggaagatt tatgcccatc cgctttttat cgatcagttt
      901 gaggctctga ttgcggaggt cgagcagctt cgggcggccg atccggaagg ctatcgggat
      961 aagaagaaga ccaaacttct tgcggcggtt caaaaaatgg ccttcgaggt gatcccgcga
     1021 aatccggcgg atcgaatgtt tttgcaaggc aacacgctgg gaccgtccta tcgtcattgg
     1081 catcgcggaa agtttttcga ggggcgctac cggctgtttt tccgttacag cagcacggcc
     1141 aaagtgatcg ttctggcttg ggtgaacgat gcggaaaccc tgcgaaccta cgggagcaag
     1201 gccgatgcgt atgcggtatt taaggcgatg ctcgccagga atcgccctcc cgacgaatgg
     1261 ggggctttgc ttaaggaatc caaggaagcc gcttcccgga cgaaaaggcc tccgaaatag
     1321 gtcgaatcag gatctggctt ctcaggccag ggtgggagcc gcttcgggcg gcggctgggg
     1381 tttttgccgc gcgcggctgc ggtccttggc cacgagcatg taaatggccg ggacgacaaa
     1441 cagggtgaac gccgtgccga tgatgaggcc ggacacgagc atgatgccga tgctgttgcg
     1501 ggcgcccgcg ccggggccgg aggcgatcat gagcgggatg ttccccgcga tcatggccac
     1561 ggaggtcatc aggatcggcc gcaggcgcgt cgccgcggct tcgatgatgg ccgcaagctt
     1621 ggccatgccc tgcgcctgga gcttgttggc gaattcgacg atcaggatgc cgttcttcga
     1681 caccaggccg acgagcgtga tcaatccgac ctggctgtaa atatttagcg tcgtcagccc
     1741 gaggacggtg aagaccagcg cgccggagat cgccagcggg accgagccga agaggatgat
     1801 cagcggatcc cggaaacttt caaactgggc ggccagcacc aggaagatga ggatgacgga
     1861 gagcaccatg gtggccagga atttgctgcc ttccacgcgg agctgccggg attggccgcc
     1921 gtaatcgatg acaaacccgg gcggaaggat cttcgccgct tccgcttcga gcaccttcaa
     1981 cgccaggtcc agcgggacgc cgggatacag cgcgccctgg atcatggccg agttcagctg
     2041 ctggaagcgc ttgagttcgc ggggctgcgt ggtttgcttg agcgtggcaa aggtcgaaag
     2101 cggcacgagc tgcttgtccg gcccggtgac gtagatgtcg tcgagctggt ccgggttcaa
     2161 ccgctcggtc cgcttgacct gcgggatgac cttgtagctg cggccctgga tgctgaagcg
     2221 gttgacgtag ttgccgccca ccatggcgga gaggtctcgg cccacttggc ccaggtccac
     2281 tccggaagcg cgcaccttgt
//
LOCUS       DATDAY010000125         2694 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_1431936, whole genome shotgun sequence.
ACCESSION   DATDAY010000125 DATDAY010000000
VERSION     DATDAY010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2694)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2694)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2694
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1431936"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..906
                     /locus_tag="VIS74_02385"
     CDS             <1..906
                     /locus_tag="VIS74_02385"
                     /inference="COORDINATES: protein motif:HMM:NF016992.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HEY5792116.1"
                     /translation="GDRMLVAAQIFPAALPAEFSHPAFAFFNASPLFAALAPNGLHLW
                     REGADLVAVFTRGEEPVFWETLPATASRAEIFSWLERAGLSLAEEEVIPQSLTFHDFT
                     GTAATPSYCESAPASPAPHPLLREALPACDWKSPARMAREASNRKKKNLRGIAEFAAA
                     VYLGSFLTFAGWAGWQHWRAAKLRDEIAAIEPEVRRLQKAGKQWDILVPTVEGSFFPL
                     EILHRIIQKMPPDGVRLTQYDFDNPKIGIQGEAKSVSAAAELLAGLSGTDGLSHVRWE
                     MPPPALLPNNTARFAISGELTDEKN"
     gene            893..1435
                     /locus_tag="VIS74_02390"
     CDS             893..1435
                     /locus_tag="VIS74_02390"
                     /inference="COORDINATES: protein motif:HMM:NF022201.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspMb/PilO family protein"
                     /protein_id="HEY5792117.1"
                     /translation="MRKISKNEIRLLLILGGAVALMANVLLLRHGIGIVMRDRDEIAA
                     LRTQYASYLHVLDQASDWKARGEWRKQHPLPIYDASKSDSDFVERVQRSLTASGLHIQ
                     EQQLKESVKKDAFVAISLNLKITGELENLVRWMTSLQKAGSYSEIRNFTLKAADDPST
                     MVATMDLFQFFAPSGARNLP"
     gene            1432..1980
                     /locus_tag="VIS74_02395"
     CDS             1432..1980
                     /locus_tag="VIS74_02395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792118.1"
                     /translation="MTTLRRIFLPAAFFALLAGANGRAGSAYDPEIYSADRYEQIWTR
                     RPFSPATPDGTGPSQGIEQQYALAGLIKMADQWVAFVLDRKTLERQAVSATANEANMQ
                     LVSVQEQADPRNSTIVLKAGSQSGTLRFDPALLSSTNAPVPANPEGSPKPSAAVTAEP
                     APASGPPSPTRVIRRTLVHLIK"
     gene            1977..>2694
                     /locus_tag="VIS74_02400"
     CDS             1977..>2694
                     /locus_tag="VIS74_02400"
                     /inference="COORDINATES: protein motif:HMM:NF045340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792119.1"
                     /translation="MNHLLRIFAALIFCLAASALHAQKNPPAAEELVKVNLPSSPVAE
                     VLSVYEMLSGKRLIRDATLSGPNLSIVVANPLPKSEAVSLLEAALILNGYTIVPVDAR
                     TVKVLGSAKNPATEGVALYAHESQLPREDQVVSYFMPLRHLPAVDAVKIFAPYATIRP
                     FGSIIAVPNANAVVITENTPLIRRLIALQRLIDIPGQKILTEFVTLKRADAERVAELV
                     GKLLDEDGKERNATVAPVTAA"
BASE COUNT          518 a          941 c          755 g          480 t
ORIGIN      
        1 ggcgaccgca tgctcgtggc cgcgcagatt tttcccgccg ctctgccggc ggaattttcc
       61 catccggcct ttgcgttttt caatgccagc ccgcttttcg ccgccctcgc gcccaatggc
      121 ctgcatctgt ggcgggaagg cgccgatctc gtggccgtct ttacgcgagg cgaagagccc
      181 gtcttctggg aaacgcttcc cgccacggcg tcccgggccg agattttctc gtggctggaa
      241 cgcgccggcc tctccctcgc ggaagaggaa gttatcccgc agtccctgac cttccacgac
      301 ttcaccggca ccgccgccac gccgagctat tgcgaaagcg cgcccgcctc ccccgccccg
      361 catcccctgc tgcgcgaggc acttcccgcc tgcgattgga aatccccggc gcgaatggcc
      421 cgggaagcct cgaaccggaa aaagaaaaac ctccggggca tcgcggagtt cgctgcggcc
      481 gtttatctcg gttcctttct gaccttcgca ggctgggcgg gctggcagca ctggcgggcc
      541 gcgaagttgc gcgacgagat cgccgccatc gagccagaag tccggcgcct gcaaaaggcc
      601 ggaaagcagt gggacattct tgtgccgacg gtggaaggat cctttttccc cctcgaaatc
      661 ctgcaccgca tcatccagaa aatgccgccc gacggcgtcc gcctcacgca gtacgatttc
      721 gacaacccga aaatcggcat ccagggagag gcgaagagcg tctccgccgc cgccgaactt
      781 ttggccggcc tttccggcac ggacgggctt tcgcacgtcc gctgggaaat gccgcccccg
      841 gcgctcctgc ccaacaacac cgcccgattc gccatttccg gcgagctgac cgatgagaaa
      901 aattagcaaa aacgaaatcc ggctgctgct gattctcggc ggagcggtcg cgctgatggc
      961 aaatgtcctg cttctgcgcc atggcatcgg gatcgtgatg cgggaccgcg acgagatcgc
     1021 cgcgctgcgg acgcaatacg ccagctatct ccatgtattg gaccaggcct ccgactggaa
     1081 ggcgcgcggc gaatggcgca agcagcaccc ccttccgatc tacgacgcct cgaagtccga
     1141 ctccgacttc gtcgaacgtg tccagcgcag cctcaccgcg tccggactgc acatccagga
     1201 acagcagttg aaagaatctg tcaaaaagga cgcattcgtg gcgatctccc tgaatctgaa
     1261 aatcacgggc gaactcgaaa atctggtccg ctggatgacg tccctgcaaa aggccggcag
     1321 ctactcggag atcaggaact tcaccctcaa ggcggccgat gatccttcaa ccatggtcgc
     1381 caccatggat cttttccaat tctttgcgcc ttcaggagcc cggaacctgc catgaccacc
     1441 cttcgccgga tcttccttcc cgcggcattc ttcgcgctcc tcgccggggc gaacggaagg
     1501 gcgggaagcg cctacgaccc ggagatttac agcgcggacc gctatgaaca gatttggacg
     1561 cgccgcccct tcagtccggc cacccccgac ggcacaggcc cttcccaggg tatcgagcag
     1621 caatatgcgc tggcgggact gatcaagatg gcggaccagt gggtcgcctt cgttctcgac
     1681 cgaaaaacgc tggaacgcca ggccgtctca gccacggcga acgaggcgaa catgcagctt
     1741 gtctcggtcc aggaacaggc cgatccccgg aattcgacca tcgtgctgaa agccggcagc
     1801 cagtccggga cgctgcgttt tgatccggca ctgctcagtt cgacaaacgc gccagttccc
     1861 gcgaatcccg agggctcccc caaacccagc gcggcggtca ccgccgagcc cgcgcctgct
     1921 tccggccctc cgtctcccac acgcgtcatt cgccgcaccc tcgtccacct gatcaaatga
     1981 accacctcct tcgaattttc gccgctttga ttttctgcct cgccgcgagc gccctacatg
     2041 cccaaaaaaa tccacctgcc gccgaggaac tggtcaaggt caacctcccc agcagcccgg
     2101 tagccgaagt gctcagcgtt tatgaaatgc tctccggcaa gcggctcatc cgtgacgcca
     2161 ccctgtcggg cccgaacctg tccatcgtgg tggccaatcc tcttcccaag agcgaggccg
     2221 tctccctcct ggaggctgct ctcatcttga acggatacac catcgtcccg gtggacgcgc
     2281 gcacggtgaa ggtcctcggc tcggccaaaa atcccgccac ggaaggagtg gcgctttatg
     2341 cccacgagtc ccagttgccc agggaagatc aggtcgtgag ctacttcatg ccgctgcgcc
     2401 accttcccgc ggtcgatgcg gtgaaaatct tcgcgcccta cgcgacgatc cgcccctttg
     2461 gcagtatcat tgcggtaccc aatgccaacg cggtggtgat caccgaaaat accccgctca
     2521 tccggcgcct catcgcgctg cagcgcctca tcgacattcc cgggcagaaa atactcaccg
     2581 agttcgtcac gctcaaacga gccgatgccg agcgtgtcgc ggaactcgtg ggcaaactgc
     2641 tggacgagga tggcaaggag cggaatgcaa cggtcgcccc ggtaacggcg gcgc
//
LOCUS       DATDAY010000126         2210 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_1327159, whole genome shotgun sequence.
ACCESSION   DATDAY010000126 DATDAY010000000
VERSION     DATDAY010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2210)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2210)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2210
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1327159"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1625)
                     /locus_tag="VIS74_02405"
     CDS             complement(<1..1625)
                     /locus_tag="VIS74_02405"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEY5792120.1"
                     /translation="MRFFLRSLKEGITRHPGLAAALCLALLLSVIGMNWGRVECWNPD
                     QMAHRKISNQGLPKSYLKPPLHTYLNYGLVVWPVERVMGGILRLDKTWQYPVLLAGSR
                     LITLAMFAASVALLYGLALGASGKTAATVLALWAATSAGFIAFNHYLTADMPLIFWML
                     ASLFMAVRAAKTGRTLDAALAGLLAGLAAADKYNGLGVAAAIPAALFAAQGWRFIRRP
                     APWVAGLAVLGGFALGNPGAVFDAQRFVEDFQYNLLTTPVYTGKTEGSGYLAFLRNFP
                     MLLGWPATVLLAAALGIAGGAAVMRRWRREEAILVAAAAVVFGFYFMTIGRFPRMATR
                     FVLPAVPFLMLCALPALRLIPWRHPALLATAAALLVYNLACCLETGRHYLDDPRMAAQ
                     EWVKARVPEGARVENTYAPAWNKLRGVRVKAYSLPQAKGRVDRFNRLFEDNEIIQKGL
                     SRYETGDSGDTFTEAALKRRNPDFITYTSFAFDYTGNEAALEFYRKLDAQSFGYRKVF
                     DQRARPIPWWSYPQNLDFIPARMVILQRDGPAS"
     gene            1661..>2210
                     /locus_tag="VIS74_02410"
     CDS             1661..>2210
                     /locus_tag="VIS74_02410"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEY5792121.1"
                     /translation="MANSEAARGGLEMTREPSISVVIPAYNRERMIGRALRSVQHQRL
                     GAREVILVDDASTDGTLETARAMMPEIRIFRQPRNAGPAAARNVGLAAAAGEWIAFLD
                     SDDEWLPDKLSRQWEALCQDPALDVCATGHWLQRAEGTRREVLPANPPDWTRALHSAQ
                     SFHGASTPLVRKALADRVGGQDE"
BASE COUNT          403 a          719 c          755 g          333 t
ORIGIN      
        1 caggaagccg gtccgtcgcg ctggaggatc accatgcggg ccgggatgaa gtcgaggttc
       61 tgcgggtaac tccaccacgg aatcggccgg gcgcgttggt caaagacctt gcggtaaccg
      121 aaggattggg cgtcgagctt ccggtagaac tccagcgccg cttcgtttcc cgtgtaatcg
      181 aaggcgaagg aggtgtaggt gatgaaatcc ggattccggc gcttcagggc cgcctcggta
      241 aaggtatccc ccgaatcgcc ggtttcgtag cggctgagcc ctttttgaat gatttcgttg
      301 tcctcgaaga gccggttgaa gcgatccacc cgtcccttgg cctgcgggag cgaataggcc
      361 ttcacgcgga ctccgcgaag tttgttccag gcgggcgcgt aggtgttttc cacgcgggcg
      421 ccttccggca cgcgggcctt cacccattcc tgggcggcca tccggggatc gtcgaggtaa
      481 tgccggccgg tttccaggca gcacgccaga ttatagacca gcaatgcggc cgccgtggcc
      541 agcagggcgg gatgccgcca ggggatcagc cgcagggcgg gcagcgcgca aagcatcagg
      601 aacggcaccg cgggcagcac aaagcgggtc gccattcgcg gaaaccggcc gatggtcata
      661 aaatagaacc cgaacaccac cgcggccgcc gcgacaagga tggcttcctc ccgccgccag
      721 cgccgcatca ccgccgcccc gccggcgatt cccagggccg ccgccagcag gacggtggcc
      781 ggccagccga gcagcatcgg aaagtttcgc aaaaaggcca ggtaaccgct gccttccgtc
      841 ttgccggtgt aaaccggggt ggtcagcagg ttgtattgga aatcctccac gaaccgctgg
      901 gcgtcgaaaa cagcccccgg attccccagc gcaaatccgc ccagcacggc caaacccgcc
      961 acccagggcg cgggccgccg gatgaagcgc cagccctggg ccgcgaacag ggcggcggga
     1021 atggccgccg ccaccccgag cccgttgtat ttgtccgccg cggccagccc ggccagcagg
     1081 cccgccaggg cggcgtcgag cgtccggccg gttttcgccg cccgcacggc catgaaaagg
     1141 gaggcgagca tccagaaaat cagcggcatg tcggcggtga ggtagtggtt gaaggcgatg
     1201 aagcccgcgc tggtcgccgc ccacagggcc aggaccgtcg cggcggtctt tcccgaggcg
     1261 cccagggcca gcccgtaaag cagggccacc gaggcggcaa acatcgcgag ggtgatcagc
     1321 cgggagccgg ccagcaggac gggatattgc cacgtcttgt ccagccgcag gatgcctccc
     1381 atgacccgtt ccaccggcca aaccaccagg ccataattta gataggtatg cagcgggggt
     1441 ttgaggtagc ttttcggcag accttgattc gagattttcc gatgcgccat ttgatccgga
     1501 ttccaacatt ccacccggcc ccagttcatg ccgatgacac tgagcagaag ggccaggcaa
     1561 agcgcggcgg cgaggccggg gtgccgcgtg attccctctt tcagggaacg caggaaaaat
     1621 cgcatccggc cacgatactt gcggattccc cggggactca atggcaaact cggaggcggc
     1681 ccgcggcggg cttgagatga cccgcgaacc ttccatcagc gtcgtcattc ccgcctacaa
     1741 ccgcgagcgg atgatcggcc gggcgctgcg cagcgtgcag catcaacggc tcggcgcgcg
     1801 cgaggtcatc ctcgtggacg acgcctccac ggatgggacg ctggagaccg cccgcgcgat
     1861 gatgccggaa atccggattt ttcgccagcc gcggaacgcg ggcccggcgg cggcccggaa
     1921 cgtgggcctg gcggcggcgg cgggcgaatg gatcgctttc ctggattccg acgacgagtg
     1981 gctgccggac aaactttccc gccaatggga ggccttgtgc caggatccgg cgctggacgt
     2041 gtgcgcgacc gggcattggc tgcagcgggc ggagggaacc cggcgggaag tccttcccgc
     2101 caatccgccg gactggacgc gcgccctgca ttccgcgcag agttttcacg gcgcctccac
     2161 gccgctggtg cgcaaggcgc tggcggaccg ggtggggggg caggacgagc
//
LOCUS       DATDAY010000127         4181 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_1608677, whole genome shotgun sequence.
ACCESSION   DATDAY010000127 DATDAY010000000
VERSION     DATDAY010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 4181)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4181)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4181
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1608677"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..793
                     /locus_tag="VIS74_02415"
     CDS             <1..793
                     /locus_tag="VIS74_02415"
                     /inference="COORDINATES: protein motif:HMM:NF012609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PEP-utilizing enzyme"
                     /protein_id="HEY5792122.1"
                     /translation="IHSTEPSPTERAERRAAEKLKKHPIRRLIFQQILRQARERVRDR
                     ENLRFERTRVFGQVRRIFVEIGRRLYAMDVLPEPRDVFYLAAEESLGFIEGASLTQDL
                     GRLAGLRKKEFAGHRERPMADRFETRGAVYAGNSFASRLAPAPPPDGDHVNGIGCCPG
                     IVRGKVRVILDPRNALIHPGEILVAPRTDPGWIMLFPAAAGLLVEHGSLLSHSAIVAR
                     EMRIPAVVSISGITSWLKTGDEVELDGSSGRVTRIRSSGGNADGK"
     gene            783..1880
                     /locus_tag="VIS74_02420"
     CDS             783..1880
                     /locus_tag="VIS74_02420"
                     /inference="COORDINATES: protein motif:HMM:NF023327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3419 family protein"
                     /protein_id="HEY5792123.1"
                     /translation="MESEAASHADFTILRYAQVWEDADILIQGLAIQPDDVCLSIASA
                     GDNALSLIGAGARRVVALDLNPAQLAALELRVAAFRTLEHGEILELAGSSPSERRLEL
                     YFKCRDCLSPPARIFWEARHSDIQAGIGQAGKFERYFKLFRTRIIPWIHNARIVDELL
                     KAKPRSAREAFYEKTWNNWRWQLLFRFFFSRTIMGRMGRDPSFFRYVEGSVAEKILSR
                     TRHALTELDPSANPYLQWILKGRHLTALPHYLRPENFEKIRAGLPRLEWRQASVEAFL
                     SKTAKGTFSKFNLSDIFEYMSADQSTALLEGLITAARSRSRIAYWNMLAPRNASEALR
                     GRIQPLTESARTLHLQDKAFFYSAFVLEEIQ"
     gene            1877..3199
                     /locus_tag="VIS74_02425"
     CDS             1877..3199
                     /locus_tag="VIS74_02425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792124.1"
                     /translation="MNPWLGIGGVIVLLGILMRLVQWAQKRGLPPELSRKSVHIGMGL
                     ICAAFPWIFSNVWPVGLLSILAVSLLGGIRLLPALREKYGAVLGGVKRTSLGEIYFPI
                     AIALTFWLAEGDRLLFVIPVLILTFADAVGALVGIRYGLSRYRTDEGQKSAEGSLAFF
                     TVAFLTAHIPLLLFTTIGRSETLLIALTLGFLVMLLEAISWRGLDNFIIPLGAFFILK
                     LYAGMTAADLLIRFAVLLALVALVILGRNRTTLSDSAVLAGALAGYLIWALAGWVWLV
                     APVILFGIYIALPSFPAEERPIQSLRAVTRVMFGGFVWLFLSELMKRPEWLLPFAVCF
                     AAHAGNIIFARLRVVRTEIGFARSYAIALLGAFLPFSLICLAAGAGILIAAWGALFLA
                     IAISVAIFSRGWTGPIIAENGFRLWLTETLVAPLASLIGLLPLLKIPS"
     gene            3196..4176
                     /locus_tag="VIS74_02430"
     CDS             3196..4176
                     /locus_tag="VIS74_02430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792125.1"
                     /translation="MTSLRLQFIHEPPANSRLSPWQPEWFQQQADAHLAVLDPDQFEV
                     ARGSLWWTHAPPHPGHRPGILGHFAAASLEAARKLLDGACERLREAGCTLAIGPMDGN
                     TWRKYRLVTEPGTEPPFFLEPANPPEWPGYFVSAGFAVLATYTSSLNSDLSKRDPRAD
                     RAAERLRKAGVAIRPLDPADFADELRKIHAVSAVSFTHNFLYTELAESTFLAQYLPIQ
                     SKVRPELVLLAEHEGRPVGYLFAIPDFAQAQRGETIDTVIGKTLAVLPGKSYGGLGVV
                     LTDELQRTAHRLGYRRVIHALQHEQNNVRNMSEFYGPVMRRYALFARALE"
BASE COUNT          720 a         1322 c         1219 g          920 t
ORIGIN      
        1 gatccattcc accgaaccgt cgcccaccga acgagcggag cgccgggcag ccgaaaaact
       61 caaaaagcat ccgattcgcc ggctcatctt tcaacaaatt ctgcggcagg cccgcgaacg
      121 ggttcgggac cgggaaaatc tccgctttga acgcacccgg gtcttcgggc aggtccggcg
      181 aatctttgtc gagatcggcc ggcgccttta cgccatggac gtattgcccg aaccccggga
      241 tgtcttttat ctcgccgccg aggaaagcct cggattcatc gaaggcgctt cgctgacgca
      301 ggatctcggc cgcctcgccg gcctccggaa aaaggaattt gccggccacc gggaaaggcc
      361 tatggccgac cgctttgaaa cccgcggagc cgtttatgcg ggcaactcct tcgcgagccg
      421 gctggcgccc gcgccgccgc ccgacggcga tcacgtcaat ggaattggct gctgccccgg
      481 catcgtccgg ggaaaggttc gggtgattct cgatccccga aatgccctca ttcatcccgg
      541 cgaaattctc gtcgctccgc gcaccgaccc cggctggatc atgctgtttc ccgccgccgc
      601 cggcttgttg gtggagcatg gcagcctgct ttcccattcc gccatcgtcg cgcgcgagat
      661 gcgcatcccg gcggtggttt caatctccgg catcacttcg tggttgaaaa ccggggatga
      721 agtggaactc gacggaagtt cgggacgcgt cacgcgcatt cggtcatctg gaggaaacgc
      781 cgatggaaag tgaggcggca agccatgcgg attttaccat cctgcgttac gcccaggtat
      841 gggaggatgc ggatattctc atccaggggc tggcgatcca gccggacgat gtctgtcttt
      901 ccatcgcctc ggcgggcgac aatgccctca gcctgatcgg agccggagcg cggcgcgtgg
      961 tcgcgttgga tctgaacccc gcccaactgg cggcgctgga acttcgagtg gccgcgtttc
     1021 gaacgctcga acacggggaa atcctggaat tggccggctc cagcccgagc gaacgccgcc
     1081 tcgagctgta tttcaaatgc cgggactgcc tttccccgcc ggcgcgaatc ttttgggaag
     1141 cccgccattc cgacatccaa gcggggatcg ggcaggcggg aaaatttgaa cgttatttca
     1201 aactatttcg aacgcggatc attccctgga ttcacaacgc cagaatcgtg gatgaattat
     1261 tgaaagccaa gccccgttcc gcgcgggagg cattttacga aaaaacatgg aacaactggc
     1321 gctggcagtt gctgtttcgg tttttctttt cccggacgat catgggacgc atgggccggg
     1381 atcccagctt tttccgatac gtggagggaa gcgttgcgga aaaaatcctt tcccgaacgc
     1441 gccatgcgct gaccgaactg gatcccagcg ccaatcccta tttgcaatgg attctcaaag
     1501 ggcggcattt gaccgccctg ccccattact tgcggccgga aaatttcgaa aaaatccgcg
     1561 caggcctgcc ccgcctggaa tggcgccaag cctcggtgga ggcgtttctt tcaaagaccg
     1621 ccaaaggcac gttcagcaaa tttaatctca gcgacatctt cgaatacatg tccgcggacc
     1681 aatccacggc gctgctggaa gggttgatca ccgccgcccg ttcccggtcg cggattgcgt
     1741 attggaacat gctcgctccg cgcaacgctt cggaagccct gcgcggccgg attcaaccgc
     1801 tcacggaatc ggcgcgaacc ctgcacttgc aggacaaggc gtttttttac agcgcctttg
     1861 tcttggagga aattcaatga atccgtggct gggaatcggc ggcgtcatcg tcctgctcgg
     1921 cattctcatg cggctcgtcc aatgggcgca aaaacggggg ctccctcccg aactgtcccg
     1981 caaatcggtt cacatcggaa tggggctcat ctgcgccgcg tttccctgga ttttctcgaa
     2041 tgtctggccg gtcgggttgc tgtccattct ggccgtgagc cttctcggcg gcattcgcct
     2101 ccttcccgcg ctgcgggaaa aatatggagc ggttctcggc ggcgtcaaaa gaacctccct
     2161 tggagaaatc tattttccca tcgccatcgc cctcacgttc tggctggcgg agggcgaccg
     2221 gctgttgttt gtgattccgg tgctgatcct cacgttcgcg gacgcggtgg gagcgctcgt
     2281 gggaatccgc tacgggcttt cccgctatcg caccgatgaa gggcagaaaa gcgcggaagg
     2341 gtcgctcgcc ttttttaccg tggcattcct gaccgcgcac atcccgctgc tgctgtttac
     2401 gacgatcggc cgttcggaaa cgctcttgat cgccctcacg ctgggctttc tcgtcatgct
     2461 gctcgaagcc atttcgtggc ggggattgga caattttatc atcccgctcg gcgcgttttt
     2521 cattctcaaa ctttatgccg gcatgacggc cgccgatctt ctgattcgtt ttgcggtgct
     2581 gctcgcgctc gttgccctgg taatccttgg ccggaaccgc accacgctga gcgacagcgc
     2641 ggtgctcgcg ggcgcgctgg ctggttatct catctgggcg ctggccggct gggtctggct
     2701 ggtcgccccg gtcattctgt tcggcattta cattgccctt ccctccttcc ccgcggagga
     2761 acgcccgatt caatccctgc gggccgtcac gcgggtcatg ttcggcggat ttgtctggct
     2821 gtttctgtcc gaactcatga agcggcccga atggctgctg cctttcgccg tttgttttgc
     2881 cgcccatgcc ggaaacatca tttttgcccg cctgcgggtg gtgcggacgg agatcggctt
     2941 cgcccgttcc tatgccatcg cgttgctggg agcgttcctt ccgttctccc tcatttgcct
     3001 cgcggcgggc gctgggattt taatcgccgc ctggggggcg ctcttcctcg ccattgccat
     3061 ttcggtggcg attttttccc ggggatggac cgggccaatc atcgccgaaa atggattccg
     3121 gctttggctc accgagaccc tggtggcgcc gctggcttcg ctgattggat tgctgcctct
     3181 cctcaaaatt ccctcatgac ttcccttcgc cttcaattca ttcacgaacc gcccgcgaac
     3241 tcccgcctgt ccccgtggca gccggaatgg tttcaacagc aggccgacgc ccatctcgcc
     3301 gtcctcgatc cggatcaatt cgaggtggcg cggggttcgt tgtggtggac gcatgctcca
     3361 ccgcatcccg ggcatcgtcc gggaatcctc ggccacttcg ccgccgcttc cctggaggcg
     3421 gcccggaaac tgctggacgg agcttgtgag cgcctgcgcg aggccggatg cacgctggcg
     3481 atcgggccga tggatggaaa cacgtggcgg aaataccgcc tggtaaccga gcctggcacc
     3541 gaaccgccct ttttcctgga gccggccaat ccacccgaat ggcccggcta ctttgtttcc
     3601 gccggatttg cggtgctcgc gacttacacg tcgtccctta attccgattt gtccaaacgc
     3661 gacccgcgcg ccgatcgggc ggcggagcgt ctgcgcaaag ccggcgttgc cattcgtccc
     3721 ctcgatcccg ctgacttcgc ggacgaattg cggaaaattc acgccgtttc cgcggtgagt
     3781 tttacccata actttcttta caccgagctc gccgaatcga cgtttctggc ccagtatctg
     3841 cccattcaat ccaaggtccg gcccgagctg gtcttgctcg cggagcacga gggacggccc
     3901 gttggctacc tttttgccat tcccgatttc gcccaagccc aacgcggcga aacgatcgat
     3961 accgtgatcg gaaagacgct ggcggtgctg ccgggcaaga gctacggcgg cctgggcgtg
     4021 gttctcaccg atgaattgca acgcaccgcc catcgcctcg gttatcgccg cgtgatccat
     4081 gcgctgcagc acgaacagaa taatgttcgc aacatgagcg aattttacgg accggtcatg
     4141 cggcggtatg ccctcttcgc ccgggcgctg gaatgaatac g
//
LOCUS       DATDAY010000128         4643 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_12596, whole genome shotgun sequence.
ACCESSION   DATDAY010000128 DATDAY010000000
VERSION     DATDAY010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 4643)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4643)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4643
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12596"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..648)
                     /locus_tag="VIS74_02435"
     CDS             complement(<1..648)
                     /locus_tag="VIS74_02435"
                     /inference="COORDINATES: protein motif:HMM:NF012974.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase N-terminal domain-containing
                     protein"
                     /protein_id="HEY5792126.1"
                     /translation="MPSDSPALTAENPVKANGAPAAKNPSRAKGSSQKAGSYLPDIHR
                     KLPQSLDAEKAVLSSILLSPNDVIDICAQRITAKHFYFPAHAQVYQIILDLWQASKPV
                     DLITLTQDLSDRGMLDQVGGAAAISEIYTFVPTAANVDYYLDILTEKGLLREIITTCT
                     EFAGRGYDELNDAKGFLDELEKDILKIGEERYRAELPTMKELVNEAIVGIEELYKN"
     gene            complement(655..1185)
                     /gene="rplI"
                     /locus_tag="VIS74_02440"
     CDS             complement(655..1185)
                     /gene="rplI"
                     /locus_tag="VIS74_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006983787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEY5792127.1"
                     /translation="MAYSQVILIDKISNLGAEADLVKVRRGYARNFLSPQGKALEVTP
                     SALRQVSHLKAKRAEREAREVTAAEELGSRISKLSLGFVLETGETGKAFGSVTAKDIQ
                     DRLVQELGGIELPRHAVTLDRAIKESGEHEVSVKLHPDVTAKVKVSIQAPVKEVVAEE
                     AEEGKSFRRKPKSDEA"
     gene            complement(1340..1672)
                     /locus_tag="VIS74_02445"
     CDS             complement(1340..1672)
                     /locus_tag="VIS74_02445"
                     /inference="COORDINATES: protein motif:HMM:NF037832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5615 family PIN-like protein"
                     /protein_id="HEY5792128.1"
                     /translation="MKLLLDENLSDRIVPRILDLFPDSTHVKLLGLTRTNDTLIWSFA
                     QQHGFTIVSKDADFHQRSLVFGHPPKFVFLRVGNCPTSRIIQLLRFSGVLLSAFESDP
                     DTSILVLP"
     gene            complement(1669..1893)
                     /locus_tag="VIS74_02450"
     CDS             complement(1669..1893)
                     /locus_tag="VIS74_02450"
                     /inference="COORDINATES: protein motif:HMM:NF016167.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HEY5792129.1"
                     /translation="MDYEHIITVEPDKRSGKPCIRGMRITVYDVLEYLASGMTEDEIL
                     ADFSELTRQDIKACLAFAADRERKLFTAAA"
     gene            complement(2029..2865)
                     /locus_tag="VIS74_02455"
     CDS             complement(2029..2865)
                     /locus_tag="VIS74_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HEY5792130.1"
                     /translation="MSDPVWPIQSVRWFSLSDRGRIRRNNEDSFLGLQFNAQEIYHLG
                     KIGEALPGAMDFVFAVSDGMGGANAGEFASRIAVEKITRRLPPAFKQKATGVDAGFSD
                     VLTELFAEIHKALTYLAWGDPDCAGMGATLTLGWLTPGWFYFAHIGDSRIYYLPKREG
                     GIKQLSHDDTYVGWLFRQGRMNEREARQHPGKNALQRALGAGHQFVEPQVGAVGYEPG
                     DQFLLCTDGLVDGLFDYQILERLRDPAYEEGEPNPARRLVNAALERSGRDNTTALLVE
                     LV"
     gene            complement(2862..3254)
                     /locus_tag="VIS74_02460"
     CDS             complement(2862..3254)
                     /locus_tag="VIS74_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HEY5792131.1"
                     /translation="MQQVKDTLRAMVRIGYDGRVHKTFRGPLAEERFANEAKVLRYLE
                     ERGCPFVPRLLSSDAEKLTLVTTSCGNRVERIDEARAREVFDELLPYGVRHDDAEARN
                     ITYRQTDGRFCVIDFEFATILDEIDEKA"
     gene            3427..4446
                     /locus_tag="VIS74_02465"
     CDS             3427..4446
                     /locus_tag="VIS74_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase beta-grasp
                     domain-containing protein"
                     /protein_id="HEY5792132.1"
                     /translation="MAKYKLEYLWLDGYQPVANLRGKTLIKEFDSFPTLEQLPNWGFD
                     GSSTRQAEGRSSDCVLKPVAVYPDSTKINGALVMCEVLLPDGSPHPSNARATIMDDSG
                     TWFGFEQEYFLYKDGRPLGFPKEGYPYPQGLYYTGVGYSSVGDIAREIVDTHLDLCLD
                     AGINHEGINAEVAKGQWEFQIFGKGSKKAADEVWVARYLLDRLCESYGVDVNYHCKPL
                     GMDVDWNGSGMHSNFSTDYMRETGGKEYFEALMAAFDKYKNEHIAVYGPDNHLRLTGL
                     HETQSIDKFNYGVANRGASIRVPHSFVNNGYKGYLEDRRPNSLGDPYKIACLILQTIA
                     TVPTK"
BASE COUNT          984 a         1401 c         1313 g          945 t
ORIGIN      
        1 gtttttgtaa agttcctcga tgccgacgat cgcctcgttg accagctcct tcatcgtcgg
       61 caattcggcg cgataccgct cttccccgat tttcaggatg tccttttcga gctcgtcgag
      121 gaatcccttc gcgtcgttga gctcatcata cccccgcccg gcgaattccg tgcaggtcgt
      181 gatgatttcg cggagcaacc ccttctcggt gaggatgtcg agataatagt ccacgttcgc
      241 cgcggtcggc acaaacgtgt aaatctcgga aatcgccgcg gcgccgccga cctgatcgag
      301 catgccgcgg tcgctgagat cctgcgtcag cgtgatgaga tcaaccggct tcgacgcctg
      361 ccatagatcg agaatgatct gataaacctg ggcgtgcgcg ggaaaataaa aatgcttcgc
      421 cgtgatccgc tgcgcgcaga tgtcgatgac atcgttcggc gaaagcagga tcgaactgag
      481 gaccgccttt tccgcatcga gactctgcgg cagcttcctg tgaatatccg gcaaataact
      541 gcccgccttt tgtgaagaac ccttcgcgcg ggaagggttc tttgcggcgg gcgccccgtt
      601 ggctttgacg ggattctcgg cggtcaaagc cggagaatca gagggcatcc cggattacgc
      661 ttcgtcggac ttgggcttgc ggcggaaaga tttgccctct tccgcttcct cggcaaccac
      721 ttccttcacc ggcgcctgga tgctgacctt gaccttcgcc gtcacgtccg gatgcagctt
      781 gacggaaact tcgtgctcgc cgctttcctt gatcgcccgg tccagcgtca ccgcgtggcg
      841 gggcagctcg atgccgccga gttcctgaac gagccggtcc tggatgtcct tcgcggtaac
      901 cgaaccaaag gccttgccgg tttcgccggt ttccaggaca aatccgaggg acaatttgct
      961 gatgcgggag cctagttcct cagccgcggt cacttcccgg gcctcgcgtt cggcgcgttt
     1021 ggccttgaga tggctcacct ggcggagggc ggaaggagtg acttcgagcg ccttgccttg
     1081 gggactcagg aaattgcgcg cataaccgcg gcggaccttc acgaggtccg cttccgcgcc
     1141 gagattggaa attttgtcga tcaggatgac ctgtgagtat gccataaaat taagtggtca
     1201 aaaagggtgg aaattatgcg gcccaccgcc cggattgtca accctgcgtt ttggaaatat
     1261 tggccaagcc gctttcccgc gatggggatg acggcggaaa gagccgcaga cgggcaatcc
     1321 gggatggtta gcttcgttgc tacggaagaa ccaagatgct ggtatccgga tcggactcga
     1381 atgccgatag gaggacgccg ctgaaacgca acaattgaat gattcggctg gttggacagt
     1441 tgccgacgcg aagaaagaca aacttcggag gatggccaaa caccaaacta cgctgatgaa
     1501 agtcagcatc tttcgagacg attgtgaagc cgtgctgctg agcgaatgac caaataagcg
     1561 tgtcgtttgt ccgggtgagt ccgagcaact tgacgtgggt tgaatcggga aagagatcaa
     1621 gaatccgcgg aacgattcgg tctgacaggt tctcatccaa caggagcttc acgcggcagc
     1681 ggtgaacagt ttgcgttcac gatcagcggc gaacgccaaa cacgctttaa tatcctgacg
     1741 ggtcaactcg gagaaatcgg ccagaatctc atcctcggtc atgccggaag cgagatactc
     1801 caggacgtca taaacggtga tgcgcatccc ccggatgcac ggcttaccgc tgcgcttgtc
     1861 gggctcaacg gtgatgatgt gctcgtagtc catggataaa acgtatctgc tggagccggt
     1921 tgcggcaaga tttcctcggg atgagagctt cgtccgccaa tcgcgccgat tttcgcggat
     1981 aaaaccgaga ctttttcaga atctgcgaaa tctgcggacg accttgcatt aaaccaactc
     2041 gaccaagagc gccgtggtat tatcccggcc ggaacgttcc agcgcggcgt tcaccaggcg
     2101 gcgggcgggg ttgggctcgc cctcctcgta ggcgggatcg cgcaggcgct cgaggatttg
     2161 gtaatcaaac aatccgtcca ccagtccgtc ggtgcagagc aggaactgat cccccggttc
     2221 gtagcccacc gccccgacct gcggctcgac gaattgatgc cccgctccca gcgcccgctg
     2281 gagggcgttc ttgccggggt gctgccgggc ctcgcgctcg ttcatccgcc cctggcggaa
     2341 cagccagccc acgtaggtat cgtcgtggct gagctgcttg atccccccct cccgtttcgg
     2401 caggtaatag atccggctgt ccccgatatg ggcgaaataa aaccagcccg gcgtgagcca
     2461 gccgagggtg agagtggcgc ccatgcccgc gcaatccgga tccccccagg ccagataggt
     2521 cagcgccttg tgaatctcgg cgaaaagctc ggtaagcacg tcggaaaaac ccgcgtccac
     2581 gccggtggcc ttctgcttga aggcgggcgg caggcggcgg gtgatttttt ccaccgcgat
     2641 ccggctggcg aattcccccg cgttggcgcc gcccatgcca tcgctcacgg caaagacaaa
     2701 atccatcgcg ccggggagag cctccccgat tttccccaga tggtaaatct cctgggcgtt
     2761 aaactgcagg cccagaaagg aatcctcatt attccggcgg attcgtcccc ggtcggacag
     2821 gctgaaccaa cgaaccgatt ggatgggcca gaccggatca ctcatgcctt ttcgtcgatt
     2881 tcgtcgagaa tggtcgcaaa ctcgaaatca atgacgcaaa aacgcccgtc ggtttgccgg
     2941 taggtgatgt tccgcgcctc ggcatcgtcg tggcgcaccc cgtagggcag caattcgtca
     3001 aagacctccc gcgcccgggc ttcatcgatc cgctcgaccc ggttgccgca gctcgtggtg
     3061 acgagggtca gcttttccgc atcggacgaa aggaggcggg ggacaaaggg gcacccccgc
     3121 tcctccagat aacgcagcac cttggcttcg ttggcaaaac gctcctccgc caggggcccc
     3181 cggaaggtct tgtgcacccg cccgtcgtaa ccgatccgaa ccatcgcccg gagagtatcc
     3241 tttacctgct gcatgcgctt accatccgga aatcgccgtg ccccgtccac tgaatctttt
     3301 ttttgcaatt ctggcattct ttctgctatc aacgggttcc cctgatgaag cgctgcaaaa
     3361 ggggaaacca aagaaaatgg cggccggcgc ccgcttaaaa aacccaagac acgataccca
     3421 aacattatgg cgaagtataa actggaatac ctctggctcg acggttatca gccggtggct
     3481 aatctccgtg gcaaaactct catcaaagaa ttcgacagct ttccgacgtt ggaacagctt
     3541 ccgaactggg gcttcgacgg aagctcgacc cgccaggccg aaggccggag ctcggattgc
     3601 gtgctgaaac ccgtcgccgt ttatcccgac agcaccaaga tcaatggcgc cctcgtcatg
     3661 tgcgaagtgc ttctcccgga cggctctccg catccgtcca atgcccgggc gacgatcatg
     3721 gacgattccg gcacctggtt tgggttcgaa caggaatatt ttctctacaa ggacggccgt
     3781 ccgctgggtt tcccgaagga aggctaccct tatccgcagg gcctctacta caccggcgtc
     3841 ggctacagct ccgtcggtga catcgcccgc gaaatcgtgg acacgcacct ggatctctgt
     3901 ctggatgccg gcatcaacca cgaaggcatc aacgcggaag tcgccaaggg ccagtgggaa
     3961 ttccagatct tcggcaaagg ctccaagaaa gccgccgacg aagtctgggt cgcccgctac
     4021 ctcctcgacc gcctgtgtga aagctacggc gtggatgtga actaccattg caagccgctc
     4081 ggcatggacg tggactggaa cggctccggc atgcactcca acttctcgac cgactacatg
     4141 cgcgaaaccg gcggcaagga atacttcgaa gccctcatgg cggcgttcga caaatacaag
     4201 aacgagcaca ttgccgtgta tggcccggac aaccacctgc gcctcaccgg cctgcacgaa
     4261 acgcagtcca ttgacaaatt taactacggc gtggccaacc gcggcgcatc catccgcgtc
     4321 ccgcacagct tcgtcaacaa cggctacaag ggctacctcg aagaccgccg cccgaattcg
     4381 ctgggagatc cctacaagat cgcctgccta atcctgcaga cgatcgccac ggttccgacc
     4441 aaataaccgt aattccgatc caaaaaaacg ccgctctgga aacggagcgg cgtttgtttt
     4501 ttcaactaaa cgggattgcc ggattcaccg gattagcggg atatttttct ccatttttat
     4561 tcatcccgtt aatccagaga atccggttaa tcccgtgtag ttgaaaaaac aaacgccgct
     4621 ccgtttccag agcggcgttg ttt
//
LOCUS       DATDAY010000129         2014 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_1114749, whole genome shotgun sequence.
ACCESSION   DATDAY010000129 DATDAY010000000
VERSION     DATDAY010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2014)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2014
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1114749"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..240)
                     /locus_tag="VIS74_02470"
     CDS             complement(<1..240)
                     /locus_tag="VIS74_02470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000651584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HEY5792133.1"
                     /translation="MSDISQLLENNRKWSERINQEKPDYFPTLSKQQSPNYLWIGCSD
                     SRVPANEILGLLPGEVFVHRNVANLVHSSDLNCLSV"
     gene            complement(282..1040)
                     /locus_tag="VIS74_02475"
     CDS             complement(282..1040)
                     /locus_tag="VIS74_02475"
                     /inference="COORDINATES: protein motif:HMM:NF019500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HEY5792134.1"
                     /translation="MSRSTPTAFPRFLREYPLGVLGFALLVTILFAPLIASLDWIFST
                     MRGPARLLPFTLLVAAAATFAIWNTARSRAITVCLASLAAILVGISSLVFHEALIAAH
                     LIAQTVFLGYAVAVVTRTVFTARVVDGNILCGAACVYLLAGVLFGYIYSFIEYLHPGS
                     FVVTASNLNLPQRQLIEAPGWLIYFSFTTLTTVGFGDILPSSNVARSAAVFEAVLGQI
                     MLVVMIARLVGLHVAHSTHDQPRRFKVGDEPDRT"
     gene            1141..1776
                     /locus_tag="VIS74_02480"
     CDS             1141..1776
                     /locus_tag="VIS74_02480"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEY5792135.1"
                     /translation="MTLLGIDIGGTGIKGALVNVKTGELVSERFRLATPQPALPNAVA
                     DAVQQIVEHFKYTGPTGVTFPAVVKEGVIYSAANVDKSWIGTNAGDLFRQHLGGPVSV
                     VNDADAAGVAEVRFGAGKNRSGVVILLTLGTGIGSAIFLDGKLLPNTEFGHLQIRGKD
                     AEKRASEKVREDKKLSWRQWSKRLSEFLEQMEKLFSPDLFILGGGVSKKAD"
BASE COUNT          439 a          560 c          656 g          359 t
ORIGIN      
        1 caccgagagg cagttgagat cggacgaatg aacgaggttc gcgacgttgc gatgcacgaa
       61 cacttcgccg ggcagcagac cgagaatctc gttcgccggc acgcggctgt ccgagcatcc
      121 gatccacaga tagttcggac tctgctgctt ggagagggtc gggaaatagt caggcttttc
      181 ctgattgatc cgctcggacc atttccggtt gttttccagg agttggctta tgtcagacat
      241 tgcagcgcat agtgttcgga agtgggcggc ggatcaaact tttatgtgcg gtccggttca
      301 tcgccgactt tgaaccgcct cggctggtcg tgggtggaat gggccacgtg caggccgacc
      361 aaccgggcga tcatgaccac gagcatgatc tggcccagca cggcttcgaa taccgccgcg
      421 ctgcgggcga cattgctcga gggtaggatg tcgccaaaac ccaccgtggt cagggtcgta
      481 aagctgaaat agatcaacca gccgggggcc tcgatcagct ggcgctgggg gagattcaga
      541 ttgctggccg tgacgacaaa actgcccgga tgcaggtatt cgatgaatga ataaatgtag
      601 ccgaacaaca ccccggccag caggtaaacg cacgcggccc cgcagaggat gttgccatcc
      661 accacccgcg cggtgaagac cgtacgggtg acgaccgcga cggcgtaacc cagaaacacg
      721 gtctgggcga tcaggtgcgc ggcgatgagg gcttcgtgga aaacgaggct gctgatcccg
      781 accaggatgg ccgccaggct ggccaggcaa accgtgatcg cccggctccg cgccgtattc
      841 caaatggcga aggtggcggc ggcggcgacc agcagggtga agggcagcag ccgggccggg
      901 ccgcgcatgg ttgaaaaaat ccagtccaga ctggcgatga gcggcgcgaa gaggatcgtc
      961 acaagcagcg cgaagcccag cacgccgagc gggtattcac gcagaaaccg gggaaatgcg
     1021 gtgggggtgg atcggctcat cagcggcctc ttttaagcag gatttggagt ttgcggcacg
     1081 gagaaagtgc gccagcaaaa gctggcgttt ccggacgggg cgaatcatac tgagccgacc
     1141 atgactctcc tcggaattga catcggcgga acgggcatca aaggtgcgct ggtcaacgta
     1201 aaaacgggtg aattggtgtc ggagcgcttc cgattggcca cgcctcagcc cgcgctgccg
     1261 aatgccgtgg cggacgcggt ccagcaaatc gtggagcatt ttaaatacac cggcccgacg
     1321 ggggtgacgt ttcccgcggt ggtgaaggag ggcgtcattt actcggcggc gaacgtggac
     1381 aagagctgga tcggcaccaa tgcgggcgat cttttccgcc agcatctggg cgggccggtc
     1441 agcgtggtta atgacgccga cgcggccgga gtcgccgagg tccggtttgg cgcgggcaaa
     1501 aatcgttccg gcgtggtcat cctgctgacc ctgggaaccg ggatcggatc cgccattttc
     1561 ctcgatggca aactgctgcc aaacacggag tttggccacc tgcaaatccg gggaaaggat
     1621 gccgagaaac gcgcctcgga gaaggttcgc gaagacaaaa aactttcctg gcggcaatgg
     1681 tccaagcggc tcagcgaatt tttggagcag atggaaaaac tcttctctcc ggacctgttc
     1741 atcctcggcg gcggggtcag caaaaaggcc gactaatttc tcgcttacct caaaaccaaa
     1801 acggaggtca tcattgtgcc ggcccagatg cacaatgaag cggggatcat cggagccgcc
     1861 tacctggcgg cccaaaacaa ggcggattga tttatttcgc cgccttctgg atccaatcga
     1921 tcaccgcggc gggtccgccg ggctggtaac ccacattgcg ggcgagttcc ttgcccccgc
     1981 tgttgagcac gaccagagtg ggaaatcctt cgat
//
LOCUS       DATDAY010000130         4157 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_1071140, whole genome shotgun sequence.
ACCESSION   DATDAY010000130 DATDAY010000000
VERSION     DATDAY010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 4157)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4157)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4157
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1071140"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..865)
                     /locus_tag="VIS74_02485"
     CDS             complement(<1..865)
                     /locus_tag="VIS74_02485"
                     /inference="COORDINATES: protein motif:HMM:NF017011.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792136.1"
                     /translation="MHPDFVVSPLDYGLWRQPGLRTFLGAPLRICVGAAAPEAVRVGW
                     GRKRSGRRAERLSARNGSRKLLLEDGFLRSIGFGPGEPPLSLVLDDRGIYYDATAPSR
                     LEALIGEPLSSAQSQRAADLMRAWREGRVSKYNQAREYRGPLPEKFVLVVDQTYGDAS
                     IHCGLASASSFARMLRAALAENPLSDIVLKTHPEVAAGRKAGHFDLAALAADSRVRLL
                     GEDVHPAGLLERAESVYTVTSQMGFEALIWQRPVRTFGMPFYAGWGLTGDELPRPNRR
                     GKATLEQLVHAA"
     gene            complement(893..1951)
                     /locus_tag="VIS74_02490"
     CDS             complement(893..1951)
                     /locus_tag="VIS74_02490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HEY5792137.1"
                     /translation="MDLEKIDWGNVIWIAVAVISLAFFIRGCSRGVIRALITLVSLVV
                     SALAGLVVGKISMLISAWLLPDWPPLFTGLIIGFGLAFGVYALFLRASEFIYGVFEDG
                     VSEMINDPSALHIRLLFGLGGGIVNFFTGLLVVWGGITLVVALGNVAEVGLLRSNITP
                     NWIEDSLINAKNAIQGDAQEGLAKGMSALPLNTADMAVKDAGTLAKDAGVFAKDAAIF
                     LQQRSAEISQAITQLVEASKDPQTMARLKENPEVRKLLENPKIAALLSSAEVNEAVAK
                     KDFVGLARNPLFLSVISDFTLAQQLIKVDFSKILNEVPAQPLPSPSASPSATPSPSAT
                     PSASPSPEPGATPVPAQS"
     gene            complement(2139..3323)
                     /locus_tag="VIS74_02495"
     CDS             complement(2139..3323)
                     /locus_tag="VIS74_02495"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEY5792138.1"
                     /translation="MYLQCDVSRLLRRYKDKITHTGIDRVNLDYAKWVIRSGGGLCIQ
                     RGHQLQTVSRAMGRVLLRPSNRARVFPPDMRRNKVIALRRLLSSLAKLGGPVKSPASA
                     IVLNTSHAWLDRTDVWSWLIKRGSRVVPFIHDLIPIEFPEYARPIEKKRHELRIDNAL
                     RHSAAIVVNSECTHSSLAAYADQTGLRLPPILVAPLGQNLKVRPHEPIPENGKPYFVF
                     LGTIEPRKNHLLILTVWREMVKRLGENTPRLIVAGRRGWECEQIVDLLDRCEQLRPHV
                     TELNNAPDEEIATLLKGARALLLPSFAEGFGMPIQEALAAGTPVIASPLPTFLEIAGD
                     IPEYAEPFDGAKWFDLIVDYAAYDSIRRREQLVRMDQFQETTWKSHFHQLESFLRSLP
                     RK"
     gene            complement(3342..>4157)
                     /locus_tag="VIS74_02500"
     CDS             complement(3342..>4157)
                     /locus_tag="VIS74_02500"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEY5792139.1"
                     /translation="TTLDNKRRYYRMLRKIIAQADHIVTVSECSKKDLIDIMGIPEEM
                     ITNTYQAVSIPAKYRDKPEDVVKREIEGTFGLKYKGYFIFYGSLEPKKNIGRMIEAYL
                     GSDIETPLVLLGAQAWKAEEELRLLDSDHIKSLIQSGNETRVKRRVIQLQYAPFPLLV
                     SLIRGAKATFFASLYEGFGLPVLESMLLGTPVLTSNTSSIPEVAGDAALMVNPYDTRE
                     MAEAMRALDENAELRASLSFKGLSQALLYSEERHEAKIAEVYSKVLGTKYFSR"
BASE COUNT          887 a         1264 c         1178 g          828 t
ORIGIN      
        1 aggcggcatg gaccaactgt tcgagcgtgg cttttccccg ccggtttgga cgaggaagct
       61 catctccggt taatccccac cccgcgtaaa agggcatgcc gaatgtccgg accggccgct
      121 gccagatcaa cgcctcgaac cccatctgcg aggtcacggt gtaaacggat tccgcccgtt
      181 ccaaaaggcc ggcggggtga acgtcctccc ccaaaagccg caccctcgaa tccgcggcca
      241 gcgcggccag atcgaaatgg ccggccttgc gcccggcggc cacttcggga tgcgttttca
      301 acacgatgtc gctcaagggg ttttccgcca gcgcggcccg cagcattcgc gcgaagctag
      361 aggcggacgc caggccgcag tggatagagg catccccgta agtctgatcc accaccagga
      421 cgaatttctc cggcagcggg ccgcgatact cccgggcctg gttgtatttc gacacccgcc
      481 cctcgcgcca ggcccgcatc aaatcagccg cccgctggct ttgggcggaa gaaagcggct
      541 cgccgatcaa tgcttccagc cgggaaggcg cggtggcgtc gtaatagatg ccccggtcat
      601 ccaacacgag ggaaagcggc ggctccccgg gaccgaaccc gatggatcga agaaaaccgt
      661 cctccagcag gagcttgcgg gacccgttcc gggcggataa acgctcggcc cgccgcccgc
      721 tgcgtttcct tccccagccg acccggaccg cttcgggagc cgcggcgccc acgcaaatgc
      781 gcaagggagc gccgagaaag gtccgcaacc ccggctggcg ccacagcccg taatccaacg
      841 gggaaaccac gaagtcggga tgcatcgaag gatggttccc gaaccggatt tcctatgatt
      901 gggcgggaac cggagtggcg cccggctccg gacttgggct ggcgctcgga gtggcgctag
      961 ggctcggagt cgcgctgggg ctggcactag ggctaggcaa gggttgggcc ggcacctcgt
     1021 tcagaatctt gctaaaatcc actttgatca gctgttgcgc caaagtgaaa tccgaaatca
     1081 cgctcaggaa aagcgggttg cgcgcgagcc cgacaaagtc cttcttggca accgcctcgt
     1141 tgacttccgc gctggacaga agggcggcga ttttcgggtt ctccagcagt ttcctgacct
     1201 ccggattttc cttgaggcgg gccatggttt gcggatcctt ggaagcctcg accaactggg
     1261 taatggcttg gctgatctcc gccgatcttt gctggaggaa aattgccgca tctttggcaa
     1321 aaactcccgc atccttggca agagttcccg catctttaac ggccatatcc gccgtattca
     1381 acggcagggc gctcattccc ttggccaagc cctcttgggc atcgccctgg atggcgttct
     1441 tggcattgat cagggaatct tcgatccagt tgggggtgat gttggatctc aacagcccca
     1501 cttcggccac gttgcccaaa gccaccacga gggtgatgcc tccccagaca acgagcaatc
     1561 ccgtgaagaa attgacaatg cctccaccca gaccaaagag aaggcggata tgcagggcgc
     1621 tgggatcgtt aatcatctcg ctgacgccat cctcaaagac gccatagata aattccgaag
     1681 cccgcaggaa aagcgcatat accccaaacg cgaggccaaa gccgatgatc aatcccgtaa
     1741 agagcggcgg ccagtccggc agaagccagg ccgaaatcaa catggagatt tttccgacga
     1801 ccaggccggc cagcgcgctc acgaccaggg ataccagcgt gatgagcgcg cggatcaccc
     1861 cgcgagagca gccccggata aagaaggcca atgaaatgac cgccaccgcg atccagatta
     1921 cgtttcccca gtcgatcttt tctaagtcca taaggtaata atttgcgagt cagctaatgc
     1981 ggtggcgtcc gtccccggtg gttgttcgag gttccggatg ccagccatgg ttttctagtt
     2041 gggcggtaag tggtcaagcc tattttgcca tcgggaagcc aagttattct gcatatccgg
     2101 cggcaaaacg gccagcaaac tctccgggtt tttgtctttc attttctggg cagggaacga
     2161 aggaaggatt cgagttgatg gaagtgggac ttccaggtgg tctcctggaa ttggtccatt
     2221 cggaccagtt gttcccggcg gcggatcgaa tcgtaggcgg cgtaatccac aatgagatcg
     2281 aaccacttcg cgccatcgaa cggctccgcg tattcgggaa tgtcgcccgc gatttccaaa
     2341 aacgtgggca agggcgaggc aatcaccgga gttcccgccg ccaaagcctc ctgaatcggc
     2401 atgccaaacc cctcggcaaa tgacggcagc aacagcgcgc gggcgccctt gagcaaagtg
     2461 gctatctcct catccggggc gttgttgagt tccgtcacgt gcggcctgag ctgctcgcac
     2521 cggtccagca aatccacgat ttgctcgcac tcccagcccc gccggcccgc cacgatcagc
     2581 cggggagtat tctcccccaa ccgtttcacc atctcccgcc agaccgtcaa aatgagcagg
     2641 tggttctttc tcggctcaat ggttcccaaa aaaacaaaat acggctttcc gttctcgggg
     2701 atgggctcat ggggccgcac tttcagattt tgcccgagcg gcgccaccaa aatcggcggc
     2761 agccgaagtc cggtttgatc ggcgtaagcg gccagcgagg aatgcgtgca ttcggaattc
     2821 accacaatcg ccgcgctatg gcgaagggca ttgtcaattc tcaactcatg cctttttttc
     2881 tcgatcgggc gggcatattc cggaaactcg atgggaatca gatcgtgaat aaagggaacc
     2941 acccggctgc cgcgtttaat cagccagctc cacacatccg tccgatccag ccaggcatgg
     3001 gaagtgttca ggacaatggc cgaggcggga gatttcaccg gaccgcccaa cttcgctagg
     3061 gaggaaagca gccggcgcag ggcgatgact ttgttccgcc gcatatccgg cggaaaaacc
     3121 cgggcccggt tggaagggcg cagcaacacc cgtcccatgg cccgggagac ggtttgcaat
     3181 tgatggccgc gctggatgca taatcctcca ccgctccgga tcacccactt ggcgtagtcc
     3241 agattgaccc gatcaatgcc cgtatgggtg atcttgtcct tataacggcg cagcaaccgg
     3301 cttacatcgc attggagata cacaatcggc taaatcatta attatcggga aaaatacttg
     3361 gttcccaaca ctttcgaata gacctcggca atcttggcct catggcgttc ctcgctgtag
     3421 agcagcgcct ggcttaatcc tttaaagctc aagctagccc gcagctccgc gttttcgtcc
     3481 agcgcgcgca tggcttcggc catttcccgc gtgtcatacg gattgaccat caacgccgcg
     3541 tcgcccgcca cttcgggaat ggagctcgta ttggaagtca gcaccggcgt tcccaacagc
     3601 atggactcca gaaccggcaa gccaaaccct tcgtaaagcg aagcgaagaa ggtcgccttc
     3661 gcgccgcgga tcaaactgac gagcagcgga aagggcgcgt attgcagctg aatgacccgg
     3721 cgcttcaccc gcgtctcatt tccggactgg atcagtgact tgatgtgatc ggaatcgagc
     3781 aaccttaact cttcctcggc tttccaagcc tgggctccca ggagcaccag cggcgtctcg
     3841 atgtcgctgc cgaggtaagc ctcgatcatg cgtccaatgt tctttttggg ttcaagcgaa
     3901 ccgtaaaaga taaaatatcc cttgtatttc aggccaaagg ttccctcgat ctcccgcttg
     3961 acgacatctt ccggcttgtc ccggtatttg gccggaatcg acaccgcctg gtaggtattg
     4021 gtgatcattt cctccggaat gcccatgata tcgatcagat cttttttgga acattccgag
     4081 accgtgacga tgtggtccgc ctgggcgatg atttttcgca gcatgcgata ataccggcgc
     4141 ttgttgtcca gagtcgt
//
LOCUS       DATDAY010000131         3098 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_2353276, whole genome shotgun sequence.
ACCESSION   DATDAY010000131 DATDAY010000000
VERSION     DATDAY010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 3098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3098)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3098
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2353276"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..384)
                     /locus_tag="VIS74_02505"
     CDS             complement(<1..384)
                     /locus_tag="VIS74_02505"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792140.1"
                     /translation="MSEALRRLAANRWIFPVLWGLGLALAFAIPQYQLLNGYTVFVLT
                     TLCINVILCASLNLVNGYMGEFSVGHAGFMALGAYASAVLTTKLLPAAQVAWLFPLVV
                     VAGGALAALIGFFLAVLSFKTRGDYL"
     gene            complement(381..1259)
                     /locus_tag="VIS74_02510"
     CDS             complement(381..1259)
                     /locus_tag="VIS74_02510"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY5792141.1"
                     /translation="MDYFSQQAINAIQLGSIYALIALGYSMVYGVLTMINFAHGDVFM
                     VGAFACLLIAALIPGVPFVLLLILVMLAIAVLGALIERFAYRPLRAAPRVSAIITALG
                     CGLVIQNTMLGFSPYPRAMPPLFPSTTYHFWGITISTLQMLIIGISIVLMLGLDFLIR
                     KTRFGIAMRAISFNSSIVPLMGVPVNQMITLTFALGAALGGAAGMLYALAYPVVSATM
                     GGLIGWKAFVAAVIGGIGNIRGAVLGAYILGSVEILTVAFLPSTYRDLIAYSLLLVIL
                     IFKPYGLLGKPPITKV"
     gene            complement(1361..2497)
                     /locus_tag="VIS74_02515"
     CDS             complement(1361..2497)
                     /locus_tag="VIS74_02515"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY5792142.1"
                     /translation="MVVAIVGLASCGPSGPAGVKIGLNVELTGEMPAVGASSKNAAEL
                     FVEQANAAGGVPVGDKAHPIQLSVGDNAAKADQAAAIAQRLISQDGVIAMVGPNASAC
                     AIPASEIAEALKCPMVTPWSTNPKTTVDSVSGKSKQYVFRACFIDTFQAVVLAKFAGN
                     DLKAKRVAVLYDVASEAPNGQATLFKKTFEADGGQIVAFETYTTGDRDFSAQLTKIRE
                     TEPDLIFLPAYYNDVPLVAQQARRLGITVPFLGSDAWSSPEIIKLGGADIDGSYFCNH
                     YSTQIATPAAQKFVADYSAKYGQAPDDVAALTYDAFGLLVEAIKHAGVLDRQAVRDAL
                     AKIPSYEGVTGTMRFEPGSGDPIKSAVVLQIKDGKFVWVANAKP"
     gene            2733..>3098
                     /locus_tag="VIS74_02520"
     CDS             2733..>3098
                     /locus_tag="VIS74_02520"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM domain-containing protein"
                     /protein_id="HEY5792143.1"
                     /translation="MNAFDWALVLSAAVLALLSAVISAAETALFALPEERRKKMRKRY
                     PAAAKRLEKLLENPAHSLHALLLADTLVNLPLIFVSLIIARKMAFTAKLPDWIVAAAV
                     FFVVILIGELIPKFLAVTMP"
BASE COUNT          672 a          964 c          892 g          570 t
ORIGIN      
        1 cagataatct ccgcgcgtct tgaaggacag caccgcgaga aaaaatccaa tgagggccgc
       61 cagcgctccg cccgccacca ccacgagggg aaacagccag gcgacctgcg cggcgggcag
      121 cagcttcgtc gtcaaaaccg ccgacgcgta ggcgcccagg gccatgaagc cggcgtggcc
      181 cactgaaaat tcacccatgt agccgttcac cagattcagg ctcgcgcaga gaatgacatt
      241 gatgcacagc gtcgtgagca cgaagaccgt gtagccgttg agcagctggt attggggaat
      301 cgcaaacgcg agcgccagcc cgaggcccca cagcaccgga aagatccagc gattcgccgc
      361 caaacgccgc aaagcctcgc tcataccttg gtgatcggcg gcttgcccag cagcccgtag
      421 ggtttgaaaa tcagaatcac cagcagcaac gagtatgcga tcaaatcgcg gtaggtggat
      481 ggcaaaaacg ccacggtcaa aatctccacc gacccaagaa tgtaggcgcc gagcaccgcg
      541 ccgcggatat tgccgatccc gccaatgacg gccgcgacga acgccttcca gccgatcaag
      601 ccgcccatcg tcgcgctcac caccggatac gcgagcgcgt agagcatgcc cgccgccccg
      661 cccagcgcgg cgcccagggc gaaggtgagc gtgatcattt ggttcaccgg cacgcccatg
      721 agcggcacga tgctggagtt aaaggaaatc gcccgcatcg cgatgccgaa acgcgttttg
      781 cggattaaaa aatccaggcc cagcatcagg acgatcgaga tgccgatgat gagcatctgc
      841 agcgtggaaa tggtgatgcc ccaaaaatga taggtcgtgc tgggaaagag cggcggcatc
      901 gcgcgggggt agggcgaaaa gcccagcatg gtattttgga tcaccaatcc gcagcccagc
      961 gccgtgatga ttgccgagac gcgcggcgcg gcgcgaagcg gccgatacgc aaaccgctcg
     1021 atcaacgccc ccagcaccgc gattgccagc atcaccaaga tgagcagcag cacgaacggc
     1081 acgccgggga tcaacgcggc gatcaacagg caggcgaaag cgccgaccat gaagacgtcg
     1141 ccgtgcgcga agttgatcat cgtcagcacc ccataaacca tgctgtaacc cagcgcgatc
     1201 agggcataaa tcgagcccaa ctggatggcg ttaatggctt gctgcgaaaa gtaatccacg
     1261 gttcggtcaa tgcgaaatag ggaatgcgaa atgcgaaatg ccggatgcgc cgccggtctt
     1321 cacttcgcat ttcccatttt gcattcctaa tttcttgaaa ctacggcttc gcgttggcca
     1381 cccagacaaa tttgccgtcc ttgatctgga gcaccaccgc gctcttgatc ggatcccccg
     1441 agcccggctc gaaacgcatc gttccagtca cgccctcgta actcggaatc ttcgccagcg
     1501 cgtcgcgcac cgcctggcgg tcgaggacgc ccgcgtgctt gatcgcctcc accaggaggc
     1561 cgaaggcgtc gtaggtcagc gccgccacgt catcgggcgc ctgcccgtat ttcgcggaat
     1621 agtccgccac gaatttttgc gccgccggcg tggcgatctg cgtggaataa tgattgcaga
     1681 aatacgagcc gtcgatgtcc gctccgccga gtttgatgat ttcgggagaa ctccacgcgt
     1741 cgctgcccag gaaggggacc gtgattccca gccgccgcgc ctgctgggcg accagcggca
     1801 catcattgta gtaggcgggc aggaaaatga ggtccggctc ggtttcgcgg attttggtca
     1861 gctgcgcgga aaagtcgcgg tcgccggtcg tgtaggtctc gaaggcaacg atctggccgc
     1921 catcggcttc aaaggttttt ttgaacagcg tggcctgccc gttcggagcc tccgacgcga
     1981 cgtcgtaaag cacggcaacc cttttcgcct tgagatcatt gccggcgaat ttggccagca
     2041 ccaccgcctg gaaggtatca ataaagcagg cccggaaaac gtattgcttg gattttccgg
     2101 agaccgaatc caccgtcgtt ttcggattcg tggaccaggg agtgaccatc ggacacttca
     2161 gcgcttcggc aatttccgaa gccggaatcg cgcaggcgct ggcattcggg cccaccatgg
     2221 cgatcacgcc gtcctgcgag atcaggcgct gggcaatcgc cgcggcttga tccgctttcg
     2281 ccgcgttatc accgacggaa agctgaatcg ggtgcgcctt gtctcccacc ggaacgccgc
     2341 ccgccgcgtt ggcttgctcg acaaataatt ccgccgcgtt tttgctggag gctcccacgg
     2401 ccggcatttc tccggtgagt tcgacattga gtccgatttt caccccagcc ggacccgagg
     2461 ggccgcagct ggccaatcca acaatggcaa caaccaaccc gggaagaaga aatttttcca
     2521 tacgcgtcag gttcgcatga agtgcaggat tggccccaca aggcaagact ctaatcaaaa
     2581 acgaggcaac cccggccgga gggcattcga taatccgaag ctggccgggc cgttcacttt
     2641 ttttcatgtc caagccgggg tctttgagtc acatttggcg cactcaaccg gcccattttt
     2701 atcgaggtga aacttttccc aatcaattcc aaatgaatgc tttcgattgg gcacttgtcc
     2761 taagcgccgc agtcctcgcc ctgctgtccg cggtcatttc cgccgccgaa actgccctgt
     2821 ttgccctgcc ggaggaacgc cgtaaaaaaa tgcggaagcg ttatccggcg gcggcgaagc
     2881 ggctggaaaa gctgctcgaa aacccggcgc actcgctgca cgccctgttg ctggcggaca
     2941 cgctggtaaa tcttcccctg atttttgttt ccctgatcat cgcccgcaaa atggccttca
     3001 ccgccaagct gccggactgg atcgtggcgg cggcggtctt ttttgtcgtc attctcattg
     3061 gcgagctgat tcccaaattc ctcgcggtca cgatgccg
//
LOCUS       DATDAY010000132         3566 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_1432719, whole genome shotgun sequence.
ACCESSION   DATDAY010000132 DATDAY010000000
VERSION     DATDAY010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 3566)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3566)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3566
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1432719"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..324)
                     /locus_tag="VIS74_02525"
     CDS             complement(<1..324)
                     /locus_tag="VIS74_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979677.1"
                     /note="catalyzes the formation of biotin from
                     dethiobiotin; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase"
                     /protein_id="HEY5792144.1"
                     /translation="MSLTYSELEAIYRRPFFDLLWDARSVHREHWPEHDVQLCTLLSV
                     KTGGCSEDCSYCAQSARYSTGVKAERLMTTEQIMPYARQARETGSTRFCMGAAWKGVR
                     CGDRKF"
     gene            complement(470..1558)
                     /locus_tag="VIS74_02530"
     CDS             complement(470..1558)
                     /locus_tag="VIS74_02530"
                     /inference="COORDINATES: protein motif:HMM:NF018613.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY5792145.1"
                     /translation="MKSILILTSAYGEGHNAAARNLAAAIRTLAPDARVEVADVFAEV
                     YGKWNRLSVRLYHFVINRLPWLWQHLFDWLHRTTFVPGQIGLFRKAARRLREKLEALK
                     PEVVVSTYPGNNFLLDHLLRRSVRRPFQTVTIVTDSITINSAWIQGHSDFWVTANAAT
                     TEIIRALGVPPAKLRTLGFPVPPEFAELGGVRPPGPPWRVLYAINAGRHLAPAIVEKL
                     LEVPGIELTVTVGRDETLAARLREVGQRAGREFPVLGWVSHMPELMATHHVLIGKAGG
                     ATVQESLAAGTPMIITQIVPGQEEGNARLVIENGAGCLAQTPEEIARTLARIFAGGAN
                     EWSRWSGAARSLGTPRGALETARFVLEQ"
     gene            complement(1566..2639)
                     /gene="corA"
                     /locus_tag="VIS74_02535"
     CDS             complement(1566..2639)
                     /gene="corA"
                     /locus_tag="VIS74_02535"
                     /inference="COORDINATES: protein motif:HMM:TIGR00383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HEY5792146.1"
                     /translation="MLRLNYQLPGTPPATLLLREDLHGRKPIVTLIRYDESDVLETQI
                     DDLADLDRYFDPEKNNWINVCGLGDLEILKNLGNRFNLHPLALEDVLNTTQRPKVEEY
                     GDTLFVVSEMVYFDGKHRLTSEQLSLFLGKNFVLTFQEEMGSDIFETIRARLRSGRGY
                     SRKMKSDYLAYALLDAAVDQLFPILEAVGDQLDESEEELLDKPNRESMRNIFKVQRML
                     LQLRRAAWPHREIFNAMMRDESGLIHPETHHFLRDCYDHSTQIIDMIETYRDLAAGLM
                     DVNFASLDARTNEIMRVLTMVSTFFLPLSFLAGVYGMNFNTDESRFNMPELNWPFGYL
                     YFWGLALMVAGGMWLFFKRKKWL"
     gene            complement(2670..>3566)
                     /locus_tag="VIS74_02540"
     CDS             complement(2670..>3566)
                     /locus_tag="VIS74_02540"
                     /inference="COORDINATES: protein motif:HMM:NF012795.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HEY5792147.1"
                     /translation="GMKISELGEDGLIARLTATLAGSGDVIAGPGDDCAVIRGAKGEP
                     YWLLKTDSVVEDIHFRRTQPPGKVGWKALCRAISDIGAMGGEPRFALISLASPGEVAV
                     EYWQKFYRGLARAARRYGVCVVGGETSRSPGPIVVTVSLAGRVRKDRLVLRRGGKPGD
                     WLFVTGRLGGSLRGRHLTFEPRVREAAWLARHFRPSAMMDLSDGIAKDLPRLAAQSRV
                     GFSIDPEAIPRNRGCGVAEALGDGEDYELLFAIPPERAEALPRAWRKKFPRLPLTRIG
                     ELCRRSSLPSFAGQGFDHFRVD"
BASE COUNT          706 a         1180 c         1061 g          619 t
ORIGIN      
        1 gaacttccgg tccccgcagc gcacgccttt ccatgccgcg cccatgcaga aacgggtgga
       61 gccggtctcg cgggcctgcc gcgcgtaggg catgatctgt tcggtcgtca tgaggcgctc
      121 cgccttcacg ccggtggaat aacgggcgct ctgcgcgcag taggagcaat cctcgctgca
      181 tccgccggtc ttcacgctca gcagcgtgca aagctggacg tcatgctccg gccaatgctc
      241 gcggtgaacg ctccgcgcat cccacagcag atcaaaaaag ggacggcggt aaatggcttc
      301 cagttcagaa taagtcagac tcacgatgcg aaaaaactac cgaatgccgg gcggggcgtc
      361 aattcatgcc gaatggaatc ccggggagac gctatcgccg gttcgatcca tcgggaaaac
      421 catcacgcaa agccgccagg gcgcaaaaag aaacgaagtt tttcttcctt tactgctcca
      481 gcacaaaccg ggcggtctcc aacgcccctc gcggggtgcc gaggctccgc gccgcgccgc
      541 tccaacggct ccattcattc gcgccgccgg cgaaaatccg ggcaagcgtc cgggcgattt
      601 cctccggggt ttgggccagg cagccggcgc cgttttcgat gaccagccgc gcattgccct
      661 cctcctggcc ggggacgatc tgggtaatga tcatcggcgt gcccgcggcg aggctctcct
      721 gcacggtggc gccgccggcc ttgccgatca atacgtgatg cgtggccatc aattccggca
      781 tgtgggacac ccagccgaga acgggaaact cgcgcccggc gcgctggccc acttcccgca
      841 accgggcggc aagcgtttcg tcgcgcccga ccgtcacggt taattcgatc ccgggaactt
      901 ccagcagttt ttccacgatg gccggagcga ggtggcggcc ggcattgatg gcataaagca
      961 cgcgccaggg cggtcccggc ggacggaccc cgcccaactc ggcgaactcc ggcggcaccg
     1021 gaaatcccag ggtgcgcaac tttgccggag gcactccgag cgcgcgaatg atttccgtcg
     1081 tggcagcatt ggcggtgacc cagaaatccg aatgcccctg gatccaggcg gagttgatgg
     1141 tgatcgaatc cgtcacaatc gtgaccgtct ggaacggccg ccgaacgctt cggcgcagga
     1201 ggtgatccag caggaagtta ttgcccggat acgtgctcac gaccacctcc ggcttgagcg
     1261 cctccaattt ttcccgcaag cgccgcgcgg ctttgcggaa gaggccgatc tgccccggca
     1321 caaacgtggt gcggtgcagc cagtcgaaca gatgctgcca gagccagggc aggcggttga
     1381 tgacgaaatg atacagccgg acggaaagcc ggttccattt tccgtaaacc tccgcaaaca
     1441 cgtcggccac ttccacccgc gcatcgggag cgagcgttcg gatggcggcg gcgagattcc
     1501 gcgcggccgc gttgtgcccc tcgccatagg ccgaggtcag gatcagtatg gatttcatcg
     1561 accgctcaca gccatttttt gcgtttgaag aacagccaca tgcccccggc aaccatcagc
     1621 gcgaggcccc aaaaatagag atacccgaac ggccaattta actccggcat gttgaaacgg
     1681 ctctcgtcgg tgttgaaatt catgccgtaa acccccgcga gaaaactcag cggcaggaaa
     1741 aaggtgctca ccatggtgag cacgcgcatg atctcattcg tccgcgcatc gaggctcgcg
     1801 aagttcacat ccatcaaccc cgcggcgagg tcgcggtagg tttcgatcat gtcaatgatc
     1861 tgcgtcgagt ggtcataaca gtcgcgcaga aaatggtgcg tttccgggtg aatcagcccg
     1921 ctctcgtcgc gcatcatggc attaaaaatc tcgcgatgcg gccaggccgc gcggcggagc
     1981 tgcagcagca tgcgctgcac cttgaagatg ttccgcatgg actcccggtt gggcttgtcg
     2041 agcaactcct cctcggattc gtcgagttgg tcgcccaccg cttcgagaat gggaaataat
     2101 tgatccaccg ccgcgtcgag cagcgcgtag gcgagataat cgctcttcat cttgcgcgag
     2161 taaccccgcc ccgaccgcag ccgagcccgg atggtttcga aaatgtcgga acccatttcc
     2221 tcctgaaagg tcagcacgaa attcttgccg agaaacaagc tgagctgttc gctcgtcagg
     2281 cggtgtttcc cgtcgaaata caccatctcg ctgaccacaa agagggtgtc cccgtattcc
     2341 tcgaccttgg gccgctgcgt ggtgttcagt acatcctcga gcgccagggg atgcaggtta
     2401 aagcggttgc ccaggttctt gagaatctcg agatcgccca ggccgcagac attgatccaa
     2461 ttatttttct ccggatcaaa ataccggtcg agatcggcca gatcatcgat ctgcgtttcc
     2521 aaaacgtccg actcatcgta gcggatcagc gtgacgatcg gttttcggcc atgcaagtcc
     2581 tcgcgcagca acagggtcgc cggcggggtt cccggcagct gataattcag gcgcaacatg
     2641 caggctaaaa gaagcccggt ccgcgcggat caatcaactc gaaaatggtc aaatccctga
     2701 ccggcgaacg acggcaaact cgagcggcgg cataattcgc caatccgcgt caacgggagg
     2761 cggggaaact ttttccgcca ggctcgcggc agcgcctccg cccgctccgg cggaatcgca
     2821 aacagcaatt cgtagtcctc gccgtcgccc aaagcctcgg ccacgccgca tccgcggttc
     2881 cgcgggatcg cctccgggtc gatggaaaag cccacccggc tttgcgccgc caggcgcggc
     2941 aggtctttgg cgatcccgtc gctcaggtcc atcatggcgc tggggcggaa atgccgcgcc
     3001 agccaggcgg cctcccggac gcggggttcg aaagtcagat gccggccgcg caatgaaccg
     3061 cccagccgcc cggtgacaaa cagccagtcg cccggcttcc cgccgcggcg cagcaccagg
     3121 cgatcctttc gcacccggcc ggcgagcgaa accgtcacca cgatcggccc cggggagcgc
     3181 gaggtctcgc cccccaccac gcaaacgccg taacgccgcg ccgcccgggc caggccgcga
     3241 tagaattttt gccagtattc cacggccacc tcgcccggcg acgccaggct gatcaaggca
     3301 aaccgtggtt cgccgcccat ggcgccaatg tcgctgatgg cccggcacag cgccttccag
     3361 ccgactttcc cgggcggctg ggtgcgccgg aaatgaatgt cctccaccac gctgtccgtc
     3421 ttcaaaagcc aatagggctc gcccttcgcc ccgcggatga cggcgcagtc gtcgccgggg
     3481 ccggcaatca cgtcgcccga acccgccagc gtggccgtca ggcgggcgat caagccatcc
     3541 tcccccagct cggagatttt cattcc
//
LOCUS       DATDAY010000133         2535 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_1803348, whole genome shotgun sequence.
ACCESSION   DATDAY010000133 DATDAY010000000
VERSION     DATDAY010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2535
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1803348"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..988
                     /locus_tag="VIS74_02545"
     CDS             <1..988
                     /locus_tag="VIS74_02545"
                     /inference="COORDINATES: protein motif:HMM:NF015094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HEY5792148.1"
                     /translation="FRKFSTEKVLTMEFMSGIRVDDRPALKRANIDPSALAERITKLI
                     YRQIFDFGFFHGDPHPGNVAIQRDGAVGLYDYGMMGTFTPDFRVSVANLISGLAEKNH
                     RQVMRSILEMSEEGYVSDPEKMLTDVESFSDQHLNHPLKDIKLGEVLNRLLELLRNHH
                     LRMRRSFYLGVKALTQVEAIGRELDPELNFILMGEPYATEVITGKYKPGRMLKLLERL
                     FAESVDFLEQFPHDFRTFYLRAKRGDFNIPLQHKIDPEGFEPLRKTLDSIANRLANAI
                     LAASVLICSSIIVLSHIPPLFHGISLIGIIGLACGGYLCLRLILSIWRHGGL"
     gene            complement(1040..1354)
                     /locus_tag="VIS74_02550"
     CDS             complement(1040..1354)
                     /locus_tag="VIS74_02550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792149.1"
                     /translation="MPAAKKKTQTAAPKKAAPKTTAQKKAAAVRSSPEEKLATNALKW
                     IDEAADLLRKGVNTTASSSAKARFEAKQKAHTLLTKAHGTLSQALTDGTSLLQKAIKK
                     LP"
     gene            1461..1937
                     /locus_tag="VIS74_02555"
     CDS             1461..1937
                     /locus_tag="VIS74_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEY5792150.1"
                     /translation="MEALIQLLQQNALIPRDEAARLLDLTVEEVNARIAQLEKDGVII
                     GYQTVINREKWNPDTVTAVIEVKITPERDGGFDRIAQRIARFDEVKSCYLVSGGYDLL
                     VMVEARTLREVAAFVAEKLSTIGAVQSTGTHFRLKTYKENGVFHHFESAPERLAVS"
     gene            1934..>2535
                     /locus_tag="VIS74_02560"
     CDS             1934..>2535
                     /locus_tag="VIS74_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY5792151.1"
                     /translation="MSIRIADHVKEIPRSGIRDFFEVVQTMADVVSLGIGEPDFATPW
                     NIREAAIYSLEKGRTGYTSNLGLPRLRRAIANYVDEHFGVRYDAPSEVLVTVGVSEAI
                     DLALRALLNPGDEVIYHEPCYVSYRPSIALAHGVPVAVATRGAEGFVLRAAEIAAKIT
                     PRTRVLMLNFPTNPTGATMPAEELEKIAALAVKHDLIVLT"
BASE COUNT          463 a          747 c          801 g          524 t
ORIGIN      
        1 tttccgcaaa ttcagcacgg aaaaggtgct gaccatggaa ttcatgagcg gaatccgggt
       61 ggacgaccgg ccggcgctca agcgcgcgaa catcgatccc tccgcgctgg ccgagcggat
      121 cacgaaattg atctaccggc agattttcga cttcggattt ttccacggcg acccgcatcc
      181 gggcaatgtg gccatccagc gcgacggcgc ggtggggctt tacgattatg gaatgatggg
      241 caccttcacc ccggatttcc gcgtgagcgt ggccaatctc atttccggcc tcgcggaaaa
      301 aaaccaccgc caggtgatgc gctccattct cgaaatgtcg gaggaaggtt acgtgagcga
      361 tccggaaaaa atgctgacgg acgtggagag tttttccgat cagcacctca accatccgct
      421 caaggacatc aagctcggcg aggtgctcaa ccggctgctg gagctgctgc gcaaccacca
      481 tctccggatg aggcgcagtt tttacctcgg cgtgaaggcg ctgacgcagg tcgaggccat
      541 cggccgcgaa ctcgacccgg agctgaattt catcctcatg ggcgagccct acgccacgga
      601 ggtgatcacc ggaaaataca agcccgggcg catgctcaag ctcttggagc ggctgtttgc
      661 ggagtccgtg gatttcctgg agcaattccc tcacgatttt cgcacgttct acctgcgggc
      721 caagcggggc gattttaata tcccgctgca gcacaagatc gatccggagg gcttcgagcc
      781 gttgcgcaaa acgctcgact cgatcgccaa ccgcctggcg aacgccattc tggccgcgtc
      841 ggtgctgatc tgttcgagca tcatcgtgct ctcgcacatc cccccgcttt ttcacggcat
      901 ctcgctgatc gggatcatcg gcctcgcctg cggcggctac ctgtgcctgc gcctgatcct
      961 ctcgatctgg cggcacggcg ggttgtgagc cggtcatggc ggggtcatcg accccgccct
     1021 acccttgaat gggaacgcct tacggcagct tcttgatcgc tttttgcagc agcgaggtgc
     1081 cgtcggtcag cgcctggctg agcgtgccgt gggctttggt cagaagcgtg tgcgccttct
     1141 gcttggcttc gaaccgggcc ttcgcgctgc tggaagccgt ggtgttcact cccttgcgga
     1201 gcaggtcggc ggcttcgtcg atccatttca acgcattggt ggcgagcttt tcctccgggc
     1261 tggagcggac ggcggcggct tttttctgag ccgtggtttt gggcgccgct tttttgggag
     1321 cggcggtttg ggttttcttt ttggcggctg gcataaaata agattacgtc tccaaatagc
     1381 gaaagccaag gcagatctgg cggcataaaa ttgcgaaggc ttgccaagcg gcggccggcg
     1441 tagcatcctg tcggtttttc atggaagcgc tcatccaact cctccaacag aatgccctca
     1501 tcccgcggga tgaggccgcg cggttgcttg atctcacggt agaggaggtg aatgcccgga
     1561 ttgcgcagct ggaaaaggat ggcgtcatca ttggttacca gaccgtcatc aatcgtgaaa
     1621 aatggaaccc ggacaccgtg acggcggtca tcgaggtgaa gatcaccccg gagcgtgatg
     1681 gcggatttga ccggatcgcc cagcgcatcg cccggttcga cgaggtgaag agctgttatt
     1741 tggtcagcgg cggttacgac ctgctcgtca tggtcgaggc gcgcacgctg cgcgaagtgg
     1801 cggcgtttgt ggccgaaaag ctcagtacca tcggagcggt gcagtccacc ggcacgcatt
     1861 tccgcctcaa gacctacaag gaaaatgggg tgttccatca cttcgaatcc gcgccggagc
     1921 ggctggcggt ctcatgagca tccgcatcgc cgatcacgtg aaggagattc cccgttcggg
     1981 aatccgggat tttttcgagg ttgtccaaac gatggccgac gtcgtctcgc tcggcatcgg
     2041 cgaaccggat tttgccacgc cgtggaatat ccgggaggcg gcgatttatt cgcttgaaaa
     2101 gggccgcacc ggttacacct cgaatctcgg cctgccgcgc ctgcgccggg cgatcgccaa
     2161 ttacgtggac gaacatttcg gggtgcggta tgacgcgccc agcgaggtgc tggtgacggt
     2221 cggcgtgtcg gaggcgatcg atctcgccct gcgcgcgctg ctgaatccgg gcgatgaggt
     2281 gatctaccac gaaccctgct acgtttccta ccgcccgagc atcgcgctgg cccatggggt
     2341 tcccgtggcg gtggccacgc ggggcgcgga gggatttgtg ctgcgggcgg cggagatcgc
     2401 cgcgaagatc accccgcgca cccgggtgct gatgctgaat ttccccacga atccgaccgg
     2461 cgcgacgatg ccggcggagg agctggaaaa aatcgccgcg ctggcggtga agcacgatct
     2521 catcgtgctc accga
//
LOCUS       DATDAY010000134         2089 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_2507732, whole genome shotgun sequence.
ACCESSION   DATDAY010000134 DATDAY010000000
VERSION     DATDAY010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2089)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2089)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2089
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2507732"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..301
                     /locus_tag="VIS74_02565"
     CDS             <1..301
                     /locus_tag="VIS74_02565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792152.1"
                     /translation="AIVGGDMANIHDDPAALKALQDDIYREKILRARAMTPEQRLADV
                     FELSNQQFGMMLAGAMHRLGTRDEAAGWREVARWMDRLDRAREYGFYATAKPADA"
     gene            298..861
                     /locus_tag="VIS74_02570"
     CDS             298..861
                     /locus_tag="VIS74_02570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792153.1"
                     /translation="MTLQDFALKVLEATETAELDFMVVGALAAGAYGVPRATKDVDLL
                     VAVTRETGLTAVMKILDPLIGFDAQVAFDTLTWGRRHVGTSRTSPPYKVEFFELFDDP
                     FVQEEFRRRREVFVPMLNRAAWLPSAEDVVVQKLRWGRSKDLDDARDILAVQGAESLD
                     MVYIEDWCARHGTTGRLQDALANIPPL"
     gene            1062..>2089
                     /locus_tag="VIS74_02575"
     CDS             1062..>2089
                     /locus_tag="VIS74_02575"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEY5792154.1"
                     /translation="MTFLRKSLLLVAGVILAALLVIAGPPVVFLAAVWWNDEREPLPA
                     EAAGRGDASRLNTGQPAEVLAIDAEAAERQVADLVLRAAREGRKISIAGARHSMGGHT
                     LYPGGIALDMLPFRNMSLDEARKIFTVGAGARWSEVIPFLDARGLAVAVMQANNDFTV
                     GGSVSVNCHGWQPSSPPIASTVEAFRLVTADGREMRCSRTENAELFSLALGGYGLFGV
                     ILNVELRVTPNAFYRAKSERVTPADYARRFRELTATDDTGLAYGRISVAPESFLREAV
                     ITTLRREETGRAFANTLTSKKPEVLKRLVFRGSVGSDYGKNLRWRLERLVGETGGAVL
                     SRNQIMNEP"
BASE COUNT          369 a          650 c          668 g          402 t
ORIGIN      
        1 cgctatcgtc ggaggagaca tggcgaacat ccacgacgat ccggccgcgc tcaaggcgct
       61 tcaggacgac atctaccggg agaaaatcct ccgcgcccgg gcgatgactc ccgagcaacg
      121 gctcgcggat gtcttcgagc tctcgaacca gcaatttggc atgatgctcg ccggggcgat
      181 gcaccgcctt ggcacgcggg atgaggcggc tggctggcga gaggtggcgc gctggatgga
      241 ccgtctcgac cgcgcccgtg aatacggctt ttatgccacc gcgaaacccg cagacgcatg
      301 acgctccagg attttgccct caaggttctt gaagcgacgg aaaccgcgga attggatttc
      361 atggtggtgg gggctctcgc ggccggcgca tacggcgttc cccgcgccac caaggacgtg
      421 gatctgttgg tggctgtgac ccgcgagacg ggtctcaccg cggtaatgaa gatcctcgat
      481 ccacttatcg gttttgacgc tcaagtggct ttcgacaccc tcacatgggg caggcgtcat
      541 gtcggcacga gccggacctc tccaccttat aaagtggaat tcttcgagct ctttgacgat
      601 ccctttgtgc aggaggaatt ccgccgccgc cgggaagttt ttgttcccat gctgaaccgc
      661 gccgcgtggc tgcccagcgc cgaggatgtt gtcgtgcaaa agctccgctg gggccgctcc
      721 aaagaccttg acgacgcccg cgacattctc gccgtgcagg gtgccgaatc gctggacatg
      781 gtctatatcg aagactggtg cgcccggcac ggcacgaccg gccgcctgca agacgccctc
      841 gccaatattc ctccgctgta aactatggct cagacgaagg tcatttcgcg gagtccgagg
      901 tagacaatcg tatagggatt ggctgccttg acaccaagat aaaaggataa cgggccttcc
      961 cggggaacaa aaagcagtcc gggagcaatt tcgggcgact gcgcgtttgt gattccaggc
     1021 tggctgccct atgcaggtgg tgcgcctgcc gcccgtttac catgactttt ctccgcaagt
     1081 ccctcctcct ggtggccggc gtcatccttg ctgccttgct ggttatcgcc ggacctccgg
     1141 tggttttcct cgccgcggtc tggtggaacg acgaacgcga acccttgccc gccgaagcgg
     1201 ccggacgcgg ggacgccagc cggttgaaca cgggccagcc ggccgaagtc ctcgccatcg
     1261 acgctgaagc ggcggaacgg caggtggctg acctcgttct tcgcgctgcc cgggagggcc
     1321 ggaaaatcag cattgccggg gcgcgtcact ccatgggcgg tcacacgctg tatccgggcg
     1381 gtatcgcgct ggacatgctg ccctttcgca acatgtccct tgatgaggcg cggaaaattt
     1441 tcaccgtcgg cgcgggggcg cgctggtcgg aggtgattcc gtttctcgac gcgcgcgggc
     1501 tggcggtggc ggtgatgcag gcgaacaacg atttcaccgt gggcggatcg gtctcggtga
     1561 actgccacgg ctggcaaccc tcttcaccgc ccattgccag cacggtggag gcgttccgcc
     1621 tcgtgacggc ggatggccgt gagatgcgct gcagccgcac ggagaacgcg gaattatttt
     1681 ccctcgcgct cggcggttac gggcttttcg gtgtgatcct taacgtcgag ctgcgcgtga
     1741 cgccgaacgc cttttaccgc gcgaagtcgg agagggtcac gccggcggac tatgcgcgcc
     1801 gtttccgcga gctaaccgct accgacgaca ccgggctggc ttacggccgc atcagcgtgg
     1861 cgccggaatc gttcctgcgc gaggcggtca ttacgaccct gcgccgcgag gaaaccggcc
     1921 gggcgttcgc caacaccctc acttcgaaaa aaccagaggt cctcaaacgc ctcgttttcc
     1981 gcggttccgt gggcagcgat tacggaaaaa atctccgctg gcgcctcgag cgtttggtcg
     2041 gcgaaacggg tggggccgtg ctttcgcgca atcagatcat gaacgaacc
//
LOCUS       DATDAY010000135         3071 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_1965043, whole genome shotgun sequence.
ACCESSION   DATDAY010000135 DATDAY010000000
VERSION     DATDAY010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 3071)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3071)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3071
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1965043"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(8..487)
                     /locus_tag="VIS74_02580"
     CDS             complement(8..487)
                     /locus_tag="VIS74_02580"
                     /inference="COORDINATES: protein motif:HMM:NF020419.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HEY5792155.1"
                     /translation="MRLVACLEKADIAWCAIGGVAVNYWAKEPMVTRDVDLVVASPSI
                     EKTRQLLEKEGFAATVFPGSINFQGHSKVSIQLSTEDFYQEFPARAVPADVHGILLRV
                     ASLEDTLAGKILAWRDPERRQSKRLKDLGDIVRLVEAHSRLWITLPDELRKLVEMPE"
     gene            complement(688..2055)
                     /gene="nuoF"
                     /locus_tag="VIS74_02585"
     CDS             complement(688..2055)
                     /gene="nuoF"
                     /locus_tag="VIS74_02585"
                     /inference="COORDINATES: protein motif:HMM:NF010120.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HEY5792156.1"
                     /translation="MPAISPIPHPKERRLIFGNLTRPGYAPDIGTYLAHGGYESLRKA
                     VTMKPGEIVEMVKAANLRGRGGAGFPCGAKWGFIKYDDGKAHYLVVNGDESEPGTFKD
                     RYILHQDPHQLIEGVIIAAFALNIQLAYIYIRCEFPQAAKIVENALREARARGLAGKN
                     ILGAGFDCDVYVHQGAAAYICGEETSMLESLEGKRPYPRIKPPYFPAVFGLYQKPTVV
                     NNVETICHIGHILRLGPEEYAKMGTPGDGGTRTLGVSGDVLRPGAYELPAGAVTLGEL
                     IYDICGGLRPGRKLKAVIPGGSSAKVLRGDEKIKLKEKQPDGSLIEKEVAITDLRMDA
                     ATMAAAGTMIGSGGVMVMDDSRNMVWALNNLNQFYAHESCGQCTPCREGSLWMSKMTT
                     RMLNGSGVANDPATLKNVADNIAGRTICAFGEACSWPTQSFVLKFPEEFAEAARGEAP
                     GGVRF"
     gene            complement(2172..2708)
                     /locus_tag="VIS74_02590"
     CDS             complement(2172..2708)
                     /locus_tag="VIS74_02590"
                     /inference="COORDINATES: protein motif:HMM:NF013426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HEY5792157.1"
                     /translation="MMTEVTPTQAELLPQSLIEEMAEAITHYPVAKRSASLPLLHLWQ
                     NHFGYIDHSGIEWIAAKLELKPIHIFELVTFYPWFRQQAPGRTIIRVCRTLACALAGS
                     YEVHESLCRAANIDPEAALHSHGGLPTSADGQFSIEFVECLASCGTAPVCLVNDDLHE
                     NVTPAGAPELLRQYEGGK"
     gene            complement(2840..>3071)
                     /locus_tag="VIS74_02595"
     CDS             complement(2840..>3071)
                     /locus_tag="VIS74_02595"
                     /inference="COORDINATES: protein motif:HMM:NF028441.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HEY5792158.1"
                     /translation="RGTAYGVVRLWVDRESGAILRMEGFDGSGRLAKRFEVITAQKLE
                     NQWFLKTMRVESFVPETGKIRDRAYLEVSVPE"
BASE COUNT          559 a          930 c          962 g          620 t
ORIGIN      
        1 gaatgggcta ctccggcatt tccaccagtt ttcggagctc gtccggaagg gtaatccaaa
       61 ggcgcgaatg ggcttccacc aaacgaacga tatcccctag atccttgagg cgtttgcttt
      121 gccggcgctc cggatcgcgc caagccagga tttttcccgc cagcgtatcc tccaaggagg
      181 ctacccggag gaggatgcca tgcacatccg ccggcaccgc ccgggcggga aattcctgat
      241 aaaaatcctc cgtgctcaac tgaatgctga cctttgaatg cccttggaaa ttgatcgacc
      301 cgggaaaaac cgtggcggca aatccttcct tctccaagag ctgccgggtt ttttcaatgg
      361 acggactcgc caccaccaaa tccacatccc gcgtgaccat gggttctttg gcccaataat
      421 tgacggccac tccgccgatg gcgcaccagg caatgtccgc cttttccaga caagcgacaa
      481 gccgcatgac atcatccgtc cccccggcgg tttgccagtc atagaattgt tttgcggtca
      541 ctcccccgca ttaccgcaag accggacgtg aaaacagcag ataaatggag ccctagattt
      601 caccgatttt tagaaaaatg acggacggct cggcgagccg tccctgcctc tcaggctttc
      661 tccgcgcctc agcagtgaaa cccgcttcta aaaccttacc ccgcccggcg cttcgccgcg
      721 ggcggcttcc gcgaattcct ccgggaactt gagcacgaaa ctctgcgtgg gccaggaaca
      781 ggcctcgccg aaggcgcaaa tggtccggcc ggcgatgttg tcggccacgt ttttgagcgt
      841 ggcgggatcg ttcgccacgc cggagccgtt gagcatgcgc gtggtcattt tcgacatcca
      901 caggctgccc tcgcggcagg gggtgcactg gccgcagctt tcgtgggcgt aaaattgatt
      961 caggttgttg agcgcccaga ccatgttgcg ggaatcgtcc atgaccatga ctccgccgct
     1021 gccgatcatg gttcccgcgg cggccatggt ggcggcatcc atccggagat cggtgatcgc
     1081 cacctccttt tcgatcaggg agccgtccgg ctgcttttcc ttgagtttga ttttttcatc
     1141 gccgcggagc accttggccg agctgccgcc ggggatgacg gccttgagct tgcgtccggg
     1201 gcggaggccg ccgcagatgt cgtagataag ctcgccgagc gtgacggcgc cggcgggcag
     1261 ctcgtaagcg ccggggcgga ggacatctcc gctgacgccg agggtgcgcg tgccgccgtc
     1321 gccgggcgtg cccattttcg cgtattcctc gggcccgagg cgcaggatgt ggccgatgtg
     1381 gcagatcgtc tccacgttgt tcacgacggt cggcttctgg taaaggccga agacggctgg
     1441 gaaatagggc ggtttgatgc gcggatacgg gcgcttgcct tccagcgact ccagcatgct
     1501 ggtttcctcg ccgcagatgt aggccgccgc gccctggtgg acatacacgt cgcaatcgaa
     1561 accggcgccg aggatgtttt tgccggcgag tccccgcgcc cgcgcctccc gcagggcgtt
     1621 ctcgacaatc ttcgcggcct gcgggaattc gcagcggatg tagatgtagg cgagttggat
     1681 gttgagggcg aaggcggcaa tgatcacgcc ctcgatgagc tggtgcggat cctggtggag
     1741 gatgtagcgg tccttgaacg tgcccggctc ggattcgtcg ccgttgacga cgaggtaatg
     1801 cgccttgccg tcgtcgtatt tgatgaatcc ccacttggcg ccgcacggga agccggcgcc
     1861 gccgcgcccg cgcaggttgg cggccttcac catttccacg atctcgccgg gtttcatcgt
     1921 caccgccttg cgcagggatt cgtagccgcc gtgggcgaga tacgtcccga tgtccggcgc
     1981 gtaacccggc cgggtcagat tcccgaaaat aaggcggcgc tccttgggat gagggatggg
     2041 ggaaatcgcg ggcatggcta gaaaatgacg aatgacgaag cccgaatgac gaaaggaacg
     2101 gtcctccgga aatcgggggt taaggatgaa aattcgggct tcatcattcg ggcttcgtca
     2161 ttcgggcttc gtcatttccc cccctcgtat tggcgcagga gttcgggggc tcccgccggg
     2221 gtcacgtttt cgtgcaggtc gtcgttcacc aggcagaccg gggcggtgcc gcagctggcg
     2281 aggcattcga cgaactcgat gctgaattgg ccgtcggcgc tcgtcgggag cccgccgtgg
     2341 gaatggagcg cggcttcggg atcgatgttc gccgcgcggc aaagcgactc gtgaacctcg
     2401 tagctgcccg cgagcgcgca ggcgagcgtg cggcagacgc gaatgatcgt tcttcccggc
     2461 gcctgctggc ggaaccaggg ataaaaggtc accagttcga aaatgtgaat cggcttgagt
     2521 tcgagctttg cggcaatcca ttcgatgccg ctgtggtcaa tgtagccgaa gtgattctgc
     2581 cagaggtgca gcagcggcag cgaggcgctg cgcttggcaa ccggataatg ggtgatggct
     2641 tccgccattt cctcaatcag ggactgggga agaagctccg cctgggtggg ggttacctcg
     2701 gtcatcatgg attcgctcgg tattggtcga atgattcccc aaaatccccg cagcgtccac
     2761 cttcaaattt gaattgaacc gccgaggcgc ggaggaaaga tgaaataaac atcctctcgg
     2821 cgtctccgcg cctcggcggt tattccggca cgctcacctc gagatacgcg cggtcgcgga
     2881 ttttgccggt ttcggggaca aaactttcca cccgcatggt tttgagaaac cattgatttt
     2941 cgagcttctg cgcggtgatg acctcgaagc gcttggccag gcggccggag ccgtcgaaac
     3001 cctccatgcg caggatggcg ccgctctccc gatccaccca cagccgcacc acgccatacg
     3061 cggtgccccg g
//
LOCUS       DATDAY010000136         2926 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_32815, whole genome shotgun sequence.
ACCESSION   DATDAY010000136 DATDAY010000000
VERSION     DATDAY010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2926)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2926)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2926
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_32815"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(553..1020)
                     /gene="rplQ"
                     /locus_tag="VIS74_02600"
     CDS             complement(553..1020)
                     /gene="rplQ"
                     /locus_tag="VIS74_02600"
                     /inference="COORDINATES: protein motif:HMM:TIGR00059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEY5792159.1"
                     /translation="MRHQKKTVKLGRSTAHRDALLANQVCSLIEHKRIKTTLAKAKAV
                     RPLAEKMVTLGKRGDLHARRTAVSYLGQKDAVKRLFEVIAPAATDRKGGYTRIIKLGP
                     RQSDSALMAYLEWVDVPVEASKEETAVEVPAGAKKAAKPKAENPAPAEKTTEE"
     gene            complement(1042..2019)
                     /locus_tag="VIS74_02605"
     CDS             complement(1042..2019)
                     /locus_tag="VIS74_02605"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006983635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEY5792160.1"
                     /translation="MPKILAKDESTATDIYAKFVAEPFEAGYGHTIGNSLRRVLLSSL
                     EGAAITSVKIEGAAHEFTSLPGVVEDVVDIILNLKKVKFKAADHEVRTVKIDLTREGA
                     ITAADIQIPQGIEIVNPNQHIVTLDKKQKFDAEFEVRVGRGFATGDENKHSDMPIGVI
                     AIDSIFSPVTRVKYAVENTRVGQRTDYDKLILEVWTDGRITPDEALLQASAILRHHLD
                     VFVSFNDEQVEFDETPQEVSQENTRLKKLLNMSVNEIELSVRAANCLNNANITTVGQL
                     AMKTKAEMLKYRNFGKKSLNEIKDKLVQLNLGLGLKFEPGLVEALPEAK"
     gene            2389..>2926
                     /locus_tag="VIS74_02610"
     CDS             2389..>2926
                     /locus_tag="VIS74_02610"
                     /inference="COORDINATES: protein motif:HMM:NF014877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792161.1"
                     /translation="MSTSHVNPLAGKPAPSSILVNLPRLLTAYFSAKPDPAISTQRAA
                     FGTSGHRGTSFAKSFNEDHIAAISQAIAEYRAAKGINGPLFLGIDTHPLSEPAQATAV
                     EVLAANGVEVVLAADPGYTPTPVISHAILKYNAGRTAGLADGIVVTPSHNPPQDGGFK
                     YNPPNGGPADTDITAVVEK"
BASE COUNT          525 a          876 c          869 g          656 t
ORIGIN      
        1 aaggcgcccg cttccaaaaa gggccgcgtt taccggcccg acgcccagct gcaccagacg
       61 gccctccggc aattgcggag gcaggagcaa ttggaaaaac tgcttttgcc cggccgccgc
      121 cggcggtgat cgccgcatag agcaactctt aaaaggaagc ttccaaaata ccaaacgcgt
      181 tgtagaggcg gttgcgccaa ccgcctgtcg taaccaaagg cgcccaatgg ggtggtgggg
      241 ccccatttcc ccacccgaca agcgcctcaa tgaaccgctt ttcggaaatc tcttttgaaa
      301 aatcctagag cggtcgccag gattcgtttc cgaaatggag ggcactgcat tgctggcctt
      361 cggtcaccct gcgggcggca cttcgtgccg gctgtctccg tcccgtcggc tgtcagtgcc
      421 cttgaattca tgggaaacgg cgaagcgttt ccctccaggt tttcggaaat ttatttcggc
      481 atctgctcta taaaaacgaa aagccggaaa cggtttcccg tttccggctt tggggcaagc
      541 gtttgaaaaa gcttactctt cggtcgtttt ctcggccgga gcggggtttt ccgccttggg
      601 cttggccgcc ttcttggctc cagcgggcac ttccaccgcg gtttcttcct tgctcgcttc
      661 caccggcaca tccacccact cgaggtaggc catcagcgcg gaatcgctct gacggggccc
      721 aagcttgatg atgcgggtgt aaccgccctt gcggtcggtc gcggcgggcg cgatgacttc
      781 aaaaagccgc ttcacggcgt ctttctggcc gaggtagctg accgccgtgc ggcgggcgtg
      841 caggtcgccg cgcttgccga gcgtgaccat tttttcggcc agtggacgga cggctttcgc
      901 cttcgcgagc gtggtcttga tgcgtttgtg ctcgatgagg ctgcagacct gattggccag
      961 cagcgcgtcg cggtgcgccg tggaccggcc gagcttcacc gtttttttct gatgtctcat
     1021 gggacgtggc ttccttgtaa cttatttggc ttcgggcagc gcttccacga ggccgggttc
     1081 aaacttcagg ccaaggccga ggttaagctg cacgagcttg tccttgatct cgttgagcga
     1141 cttcttgccg aagttgcggt atttgagcat ctcggctttg gtcttcatgg cgagctggcc
     1201 gaccgtggtg atgttggcgt tgttcaggca attggccgcg cgaacggaaa gttcgatctc
     1261 gttgacgctc atgttgagga gcttcttgag acgggtgttc tcctggctga cttcctgcgg
     1321 ggtttcgtcg aattcgacct gctcgtcatt gaagctcacg aacacgtcga ggtggtggcg
     1381 caggatggcg gacgcctgca ggagcgcctc gtcgggggtg atgcgtccat ccgtccagac
     1441 ttcgagaatg agtttgtcgt agtccgtgcg ctggccgacg cgggtgtttt ccaccgcata
     1501 cttgacgcgg gtgacgggcg agaagatgga gtcgatcgcg atcacgccga tcggcatgtc
     1561 ggaatgtttg ttttcgtcgc cggtggcgaa gccgcggccc acgcgcactt cgaattccgc
     1621 gtcgaacttc tgcttcttgt cgagcgtgac gatgtgctgg ttgggattga cgatctcgat
     1681 gccctgcggg atctgaatat cggcggcggt gatggcgcct tcgcgggtca agtcgatctt
     1741 caccgtgcgg acttcgtggt ccgcggcctt gaatttaacc ttcttgagat tgaggatgat
     1801 gtccaccacg tcttcgacca cgccgggaag gctggtgaat tcatgggcgg cgccttcgat
     1861 tttcaccgag gtgatggccg cgccttcgag cgaggaaagc agcacgcggc ggagcgagtt
     1921 gccgatggtg tggccgtaac ccgcttcaaa gggttcggca acgaatttgg cgtagatatc
     1981 cgtcgccgtg gattcatcct tggcgaggat tttgggcatt tcgaatcttc cgagacgaat
     2041 aggcatgtgt attttaagga tccgatggga atcggacgtt ctttctccag ttgccgggtt
     2101 acccctggcg cttaatggca ggctcccaaa gagagccgcc cagggaccaa cggcggattt
     2161 tgttcagcgc agagggaaaa cggtagtgga atccgcccaa aaggcaagca gggattttcc
     2221 gcaaccttct ttaatctgtc tttttaaccg caaagaacgc agagacccaa agaaaaactg
     2281 aaatgatcag gcatcccttt ccggtttcct ctattctccg cgttctttgc ggttaatccc
     2341 atcaatccga ttgaggtcgg ccgcggcttc atttaatcct gctccttgat gagcacatcc
     2401 cacgtgaatc cgctcgccgg gaagccggct ccctcgtcca ttctcgtcaa tctgccgcgg
     2461 ctgctgaccg cgtatttttc ggcaaagccc gaccccgcca tttccaccca gcgcgccgcc
     2521 ttcggcacct ccgggcaccg cggcacgtcg ttcgcgaaat ccttcaacga ggaccacatc
     2581 gccgccatca gccaggcgat cgcggaatac cgcgcggcga agggaatcaa cggcccgctt
     2641 ttcctcggca tcgacacgca tccgctttcc gaacccgcgc aggcgaccgc cgtcgaagtg
     2701 ctcgcggcca atggcgtgga ggtcgtcctc gccgccgatc ccggctacac gccgacgccg
     2761 gtcatttccc acgcgatttt gaaatacaac gccggccgca ccgcggggct ggccgacggc
     2821 attgtcgtca ccccctcgca caatccgccg caggacggcg gcttcaaata caacccgccc
     2881 aacggcggcc cggcggatac cgacatcacc gccgtcgtcg aaaaac
//
LOCUS       DATDAY010000137         2424 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_2132804, whole genome shotgun sequence.
ACCESSION   DATDAY010000137 DATDAY010000000
VERSION     DATDAY010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2424)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2424)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2424
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2132804"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1359
                     /gene="murJ"
                     /locus_tag="VIS74_02615"
     CDS             <1..1359
                     /gene="murJ"
                     /locus_tag="VIS74_02615"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HEY5792162.1"
                     /translation="EKEGDAPAWALASKMLTLAAVFMSGISLLGVLFARPLIGLLAPG
                     FAPEDAALTIWLTQIMYPFILLVSLAALVMGMLNSKNIFGVPAMASSFFNLGSIIGGV
                     WIGWSLDPRFGETALTGLAIGTLAGGLLQFAIQLPSLRKAGFRFRPDFRWKDEGVRKI
                     LILMIPSVIAASAVQVNVMVNSSFASIVGKEAVAWLNSAFRFMQMPLGMFGVAVATIT
                     LPVISRIAAHADRSDFGPTLGKAMRLSLFLTLPSAAGLAVLAEPIINLIYRWGAYTAS
                     DVSQTAHALRFYVAGLVAYACIKVLSPAFYALDRKWTPMIVSFASIGVNLGLNYLFIF
                     KFGWGHWGLALSTSLSATGNFLALYGLMHGHAGTLATRRLLTSALKCGFASLALAAAG
                     WGLLIYLREYLFHPNLIFRAAATLGSVGLCGAVYAAACWLLRVEEMRTAFDAILRKVR
                     RR"
     gene            complement(1453..1857)
                     /locus_tag="VIS74_02620"
     CDS             complement(1453..1857)
                     /locus_tag="VIS74_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEY5792163.1"
                     /translation="MVLTWLTKYREFGLLLLRIGLGVMFFYHGWPKLSGGPEVWTKVG
                     SAMSAVGVTFLPVFWGFMAAFSETVGAVLLVLGFLFRPACMLLTITMAVALTMHLKHG
                     DDFANWSHAAELGIVFLSLLLIGPGKFSVDRS"
     gene            complement(1996..2196)
                     /gene="thiS"
                     /locus_tag="VIS74_02625"
     CDS             complement(1996..2196)
                     /gene="thiS"
                     /locus_tag="VIS74_02625"
                     /inference="COORDINATES: protein motif:HMM:TIGR01683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HEY5792164.1"
                     /translation="MVNIQINGGRQDVRAAEVAGLMAELGLDPRTVLVEHNGRALHRR
                     EWPQVALAADDRIEILRVVAGG"
     gene            complement(2189..>2424)
                     /locus_tag="VIS74_02630"
     CDS             complement(2189..>2424)
                     /locus_tag="VIS74_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HEY5792165.1"
                     /translation="MSAGSHTEPGGYTGQGVENLHRTLKGSRIVALETPADSATGQFG
                     ISDGRRPAEVAEVLRTLGLEPVWKDWDAAILHG"
BASE COUNT          411 a          785 c          743 g          485 t
ORIGIN      
        1 gaaaaggaag gcgacgcccc ggcgtgggcg ctggcgtcga agatgctcac gctggcggcg
       61 gtgttcatga gcggcatcag cctgctcgga gtcctctttg cgcggccgtt gatcggcctg
      121 ctggcgccgg gattcgcgcc ggaggacgcg gcgctcacga tctggctgac gcagatcatg
      181 tatcccttca tcctgctcgt ctcgctggcg gcgctggtga tgggcatgct caattccaaa
      241 aatattttcg gcgtgccggc catggcctcg agctttttca acctcggctc catcattggc
      301 ggcgtctgga tcggctggtc gctggatccc cggtttggcg aaaccgccct gaccggcctg
      361 gccattggca cgctcgccgg cgggcttttg caattcgcca ttcagcttcc cagcctgcgg
      421 aaggcgggtt ttcggttccg cccggatttc cgctggaagg acgagggcgt gcggaagatt
      481 ttgatcctga tgattccttc ggtcatcgcc gccagcgcgg tgcaggtgaa tgtgatggtc
      541 aacagcagtt tcgcgtccat cgtcggcaag gaggccgtgg cgtggctcaa cagcgcgttc
      601 cgcttcatgc agatgccgct gggcatgttt ggcgtggcgg tggcaaccat cacgctgccg
      661 gtcatctccc ggatcgccgc gcacgcggac cgctcggact tcggcccgac gctcggcaag
      721 gccatgcggc tctcgctctt tcttaccctg ccctccgccg ccggcctggc ggtgctcgcg
      781 gaaccaatca tcaatctcat ctaccgctgg ggcgcctaca ccgcctccga cgtgtcccag
      841 accgcccacg cgctgcgttt ctacgtcgcc gggctggtcg cctatgcgtg catcaaggtg
      901 ctttcgcccg cgttctacgc cctggatcga aagtggacgc cgatgatcgt cagcttcgcc
      961 tcgatcggcg ttaatctggg attgaactac ctgttcatct ttaaattcgg ctggggccac
     1021 tgggggctgg cgctgtccac gtcactgtcg gccacgggaa atttcctcgc gctttacggt
     1081 ttgatgcacg gccatgccgg cacgctggcg acccgccggc tgctgacgtc ggccctcaag
     1141 tgcggtttcg cctcgctggc gctcgccgcc gcgggctggg ggctgctgat ctacctgcgg
     1201 gaatatttat tccatccgaa tctgatcttt cgggcggccg ccacgctggg atcggtcggc
     1261 ctctgcggcg cggtttacgc cgcggcctgc tggctgctgc gcgtggagga aatgcgcacc
     1321 gcgttcgacg ccattttgcg gaaagtccgc cggcggtagg aggaattaac cacagagatt
     1381 tggaaaaaga atggcaatca accgcctggc ctttctctgt gctctttgtt ctctgcggtt
     1441 aaaaaatccg gattaactcc ggtccacgct gaactttccg gggccgatca ggaggaggct
     1501 caggaaaacg atcccgagtt ccgccgcgtg cgaccaattc gcgaaatcgt ccccgtgctt
     1561 gaggtgcatg gtcagcgcca cggccatggt gatggtcaga agcatgcagg ccggccggaa
     1621 cagaaacccc agcacgagca gcacggctcc caccgtttcg gaaaacgccg ccatgaatcc
     1681 ccagaacacc ggcagaaacg tgacgcccac cgcgctcatg gccgaaccca ccttggtcca
     1741 gacctccggc ccgccggaaa gtttgggcca gccgtgatag aaaaacatca ctcccaggcc
     1801 gatgcgaagc agcagcagcc cgaattcccg gtatttggta agccaagtca gaaccatggg
     1861 gcggaaaact ggcgggaatc cgccaaaaag gcgagcggat aaatcattga acagaaggac
     1921 accaagacca caaaggatca acatcctcct ctttttgtgt gctttgcgtc cttctgttca
     1981 aaaactctcc ttgttctatc cccccgccac gacccggagg atttcgatcc ggtcatcggc
     2041 ggccagcgcc acctggggcc attcgcggcg gtgaagcgcg cggccattgt gctcgaccag
     2101 cacggtgcgg ggatcgagcc cgagttccgc catcaatccg gccacctccg ccgcccgcac
     2161 gtcctggcgg ccgccattga tctggatgtt aaccatggag aatcgcggcg tcccagtcct
     2221 tccagacggg ctccaggccg agggttcgga gcacctccgc cacttcggcg ggccggcggc
     2281 cgtcggaaat gccgaactgg ccggtggccg aatccgccgg ggtttccagc gccacgatgc
     2341 ggctgccctt gagggttcga tgcaaattct ccacgccctg cccggtgtag ccgccgggtt
     2401 cggtgtggct gccggcgctc atca
//
LOCUS       DATDAY010000138         3338 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_55738, whole genome shotgun sequence.
ACCESSION   DATDAY010000138 DATDAY010000000
VERSION     DATDAY010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 3338)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3338)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3338
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55738"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..598
                     /locus_tag="VIS74_02635"
     CDS             <1..598
                     /locus_tag="VIS74_02635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792166.1"
                     /translation="FWFSPYLYDNLPTINGAWLEIVNPENRDELKTFSILTMSDRLIG
                     LKTLRESTGYTWTGRFGNKIGLEDANSTKEYGSHDGINQVLLELGILGLLLCIAFLVF
                     ATLYAHKRILSCGDPQAIRIGAICMAFLSPFIAANLLTGSTIHVTPINFWMFTLIGTA
                     FTAARLAQEPKEETPLSAPAETIHSPRAGVELTKGSPV"
     gene            603..1304
                     /locus_tag="VIS74_02640"
     CDS             603..1304
                     /locus_tag="VIS74_02640"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY5792167.1"
                     /translation="MRYSTKRRLRGIAYSAQSARWLTQGSVIKHFWQGKVSPNENYLD
                     VGCGGGTYAIEFFLKRGAQATLIEYDPVLRKLAEDQVSQTGFAAQARILNGDAENLEL
                     PASHFAGIQCIEVLEHLHHPAAALQGMRAAAKAGAWLVVSVPHPPEWEPNEGHVKEGY
                     TREELVQLIESSGWKVVETRYCMLLLSRLFAWLAARGLKPHGLNWLLGIENWIPESLR
                     SHFLPYDIVALAKAV"
     gene            complement(1330..2082)
                     /locus_tag="VIS74_02645"
     CDS             complement(1330..2082)
                     /locus_tag="VIS74_02645"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY5792168.1"
                     /translation="MKTYYEDYWSKNAEWSPSAGGLTPTEKKLLLPLLKPKTVFLDYG
                     CGDGRRYGKTLTELGVDYRGFDVSSKAVEDARADGVNAALLINGFQTSLADESCEVAA
                     CFEVLEHLMEPDLAVREILRCLAPDGTGIFSVPNAANWFQRMEFALTGFWNPGGSPLT
                     GRRSPWRDPHIRFFSPTVLRRLLLESGFAEVQLRGDPFSLKNAPYFYRAPKAGKLVDG
                     LSWPLRWLGLVFPSLFSTRIFAAARKTSNSQA"
     gene            complement(2133..>3338)
                     /locus_tag="VIS74_02650"
     CDS             complement(2133..>3338)
                     /locus_tag="VIS74_02650"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY5792169.1"
                     /translation="NHCGMDSSGKSRVIFVDPNFRRSSPAMRSIVLALPFLEQAGIEV
                     EAWAHQVEPDAGVGRWHKFAFPRLPAPLNYWLFSFQATLYRLLVFPRRPRAERRRTVI
                     ISTGFYSWIADYTFIHFSNLDWFCRQRQLGVGNLRDALEWVRSLVSLGVDYLTANNPW
                     SGGLCPVSRSVAADFQRLAQYPKTIRVLPNARNERGFSPAQRLENRPAARSHHGFGSA
                     DAVFAMTAMGHLRRKGFYNAIRGLQELRKGSGRKDVRFLFIGGTAPVLAAVRDWLEQT
                     IPDWKEWVRLTGMVDNVPFHLSAADAFLFPSYSEAFSLAEIEASSMGLRLYLTPHHGS
                     EMILEPEMNGRLIPWEPDGIARILREDLDAGRVKCHSGFVGQSHSLEEYGARLVDLVQ
                     TALAGRAPL"
BASE COUNT          730 a         1028 c          900 g          680 t
ORIGIN      
        1 gttttggttt tcgccctatc tctacgacaa cttgcccacc atcaacggcg catggctcga
       61 gatcgtgaat ccggaaaatc gggatgagtt gaaaaccttc agcattctga ccatgtccga
      121 ccgactgatc gggttgaaga ccctgaggga aagcaccggt tacacgtgga ccggacgctt
      181 tggcaacaag atcggtttgg aggatgccaa ttcgaccaag gaatatggaa gccatgacgg
      241 catcaaccaa gtgcttctgg agctgggtat tttgggctta ttgctctgca tcgcttttct
      301 ggttttcgcg acgctttacg ctcataaacg gatcctgagt tgcggggatc cgcaagccat
      361 tcggattggc gccatctgca tggcgttttt aagcccattc atcgcggcca atctattgac
      421 cgggagcacc attcacgtta ccccgatcaa cttttggatg tttaccctca tcggaaccgc
      481 ctttaccgcg gctcggctcg cccaagagcc caaggaagaa actccgcttt ccgctcctgc
      541 cgagacgatt cattcccccc gtgctggcgt ggaactgacg aaggggtcgc ccgtttaagt
      601 ttatgcgtta ttccaccaag cgccggctgc gcggcattgc ctacagtgcc cagagcgccc
      661 gctggcttac ccagggttca gtcatcaaac atttttggca gggcaaagtc tcgccgaacg
      721 aaaattacct ggatgtcgga tgcggcggcg gcacatacgc gattgaattt ttcttgaagc
      781 gaggagccca ggctaccctg atcgaatatg atcccgtcct gcgaaaactc gcggaggatc
      841 aagtcagcca aacgggattc gccgcccagg cccggatcct caatggagat gccgaaaatc
      901 ttgagctccc cgccagccac ttcgccggca ttcaatgcat cgaggttctc gagcatctgc
      961 atcatccggc tgccgccttg cagggcatgc gggccgccgc caaagccggc gcctggctgg
     1021 tggtctcggt tccgcatccg ccggaatggg aacccaacga aggccacgtc aaggaaggtt
     1081 acacccggga ggaattggtt cagctgatcg aatccagcgg ctggaaagtc gtcgaaaccc
     1141 ggtattgcat gctcctgctc tcccgtcttt tcgcctggct ggccgcccgt ggtctcaagc
     1201 ctcatggttt gaattggctg ctgggcattg agaattggat ccccgaatcc ctgcgttccc
     1261 actttttgcc ttacgacatc gttgccctgg cgaaggccgt ttgattcttc cccgggttat
     1321 ccgtgcgcat caggcctggc tatttgatgt cttgcgggcg gcggcaaaaa ttcgtgtgct
     1381 gaacaaggag gggaaaacca agcccagcca gcgcagcggc cagctcagcc cgtccacaag
     1441 cttcccggct ttcggagccc ggtaaaaata gggggcgttt ttgaggctga aaggatcccc
     1501 gcgaagctgc acctcggcaa aaccgctctc caaaaggagc cggcggagaa cggtcggcga
     1561 aaagaaccgg atgtgcggat cccgccaggg actccttcgg cccgtcaatg ggcttccccc
     1621 ggggttccag aaaccggtga gcgcaaattc cattcgttga aaccagttcg ccgcgttggg
     1681 gacgctgaat atgcccgtgc catccggcgc caagcaccgc aaaatttccc ggaccgccaa
     1741 gtcgggctcc atcaaatgct cgagaacctc gaagcaggcc gccacctcgc aactttcatc
     1801 cgccagcgag gtttggaatc cattgatcag caacgccgcg ttcacgccat cggctctcgc
     1861 atcctcgacg gctttggagg agacatcgaa tccccgataa tccaccccca gctcggtcag
     1921 cgtctttccg taacggcggc catccccgca tccgtaatcc agaaataccg ttttcggctt
     1981 cagcaaaggc agaagcagtt ttttttcggt gggagtcaac ccgcccgcgc tgggcgacca
     2041 ttccgcgttt ttgctccagt agtcctcgta ataagttttc atgttgaata ctcgacgttc
     2101 aaattcctga cagggtggga atcttttccc attcaaagtg gagcccggcc agccaaggcc
     2161 gtctgaacca aatccaccag cctggcgcca tattcttcca aggagtggga ctggccgaca
     2221 aagccggagt ggcatttcac ccgcccggca tcaaggtctt cccgcagaat ccgcgcaatc
     2281 ccatccggct cccagggaat caagcgtcca ttcatttccg gctcgagaat catttcactt
     2341 ccatggtgcg gtgtcagata cagccgaagc cccatgctgc tggcttcgat ctccgccagc
     2401 gaaaaggctt cggagtagga aggaaaaaga aaggcatccg ccgcgcttaa atgaaacggt
     2461 acgttgtcca ccatgcccgt taacctgacc cattccttcc aatccggaat cgtttgctcc
     2521 agccaatccc tcaccgccgc aagcaccggc gcggttccgc cgatgaacag gaagcggaca
     2581 tcctttcggc cgctcccctt tcgaagctcc tgaaggcccc gaatggcatt ataaaatccc
     2641 ttccggcgca gatgccccat cgccgtcatg gcaaaaacgg catccgcact tccaaagccg
     2701 tgatggcttc tcgcggcggg ccggttttcg agccgctggg ccggggaaaa ccccctctca
     2761 ttccgcgcat tgggcaaaac ccgaatcgtt ttcgggtatt gcgcgagccg ctgaaaatcg
     2821 gccgccaccg aacggctgac cggacaaaga ccgccggacc agggattgtt tgcggtcaga
     2881 taatccacac ccaacgaaac caggctccga acccactcaa gcgcgtcgcg gagattgccg
     2941 actcccagct gccgttgccg gcaaaaccaa tccaggttgg aaaaatggat gaaggtgtaa
     3001 tcggcgatcc agctgtaaaa cccggtcgaa ataatcaccg ttcgcctcct ttccgcccga
     3061 ggacgccggg gaaacaccag caagcggtaa agggtggcct ggaaggaaaa gagccaatag
     3121 ttcaaaggag cgggcagccg gggaaacgcg aacttgtgcc accgccccac tccggcgtct
     3181 ggctcgactt gatgggccca tgcttcgact tcgatgccgg cctgttcgag aaacggaagg
     3241 gccagcacaa tgctccgcat cgccgggctt gatcgccgaa aattgggatc cacaaagatc
     3301 acccgcgact taccggagga atccatgccg cagtggtt
//
LOCUS       DATDAY010000139         2389 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_2164225, whole genome shotgun sequence.
ACCESSION   DATDAY010000139 DATDAY010000000
VERSION     DATDAY010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2389)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2389)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2389
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2164225"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            30..1703
                     /locus_tag="VIS74_02655"
     CDS             30..1703
                     /locus_tag="VIS74_02655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792170.1"
                     /translation="MRIAVGFTTGAFAACTISVFGALAVGTLTPALAWLSLLAGALLG
                     LLAARQTAAEPAPGPRFWDWAAGLLFAAVSLRSFLWLLFEANGELFIQSRYNLGDLAL
                     HLNFIQYFASGAPFWPESPIFQGEPLRYPIGPDLFNSLLLLAGVPLQPGLVWAGLIGA
                     GLTAHALWRWGGAFGVASLLMVGGLAGISFFHTGILADYLAHMEWKNPFLSLLVPQRA
                     FLFALPAGLILLQHWRRLLSGEPGRLPRWVEVLLYAAMPLFSVHTFLFLSGALLLRFW
                     IHRNSRASALRCVAIAFVPATILVWLVSGGFGGKSFIGWHPGWMQSGNPWAFWFWNFG
                     LMIPLMVGAVIVATRERDRAAAPFVFAGAFFFLLCGFVRFSPWPWDNTKLMLWSWVAM
                     APFIWKKLLLPLPVWGRAGVCGVLFFTGFLSLLGAMGSENRFSFARVDELHAAEAAVQ
                     AIPPAARFVIKPDYNHPLLLLGRPVVCGYDGHLWSHGYDYGAKYEAAEAAMRGQISWP
                     EAAKAFDFDYVFSGPREEAAYGQSAEPMPQVIPKTELISDGGFIEPAPP"
     gene            complement(1769..>2389)
                     /locus_tag="VIS74_02660"
     CDS             complement(1769..>2389)
                     /locus_tag="VIS74_02660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792171.1"
                     /translation="IAAFIFGSAIFAGSPSLNASTQLQVQVYHSDFLATRALDRVNIY
                     WARTALYWLLCLVPIFIWLGLAFFKPAFVLEVKPQTADGYLTALPGSFFQQAGKSGNS
                     VLMVPLGGFWLKLWASTLLVYAVTGWQAFIFLILPSPRRRWWYWSAFVICLALLVIFP
                     IYARQLMESAFLFFVNHPAGFLVPLVFLAPAALEFGRRRFLRQEFI"
BASE COUNT          406 a          752 c          753 g          478 t
ORIGIN      
        1 gcttggcaca cgcgcctcta caacgtcgca tgagaatcgc ggttgggttt acaacgggcg
       61 cgttcgccgc gtgcacaatc tcggtctttg gcgcgctggc cgtgggaacg cttactccgg
      121 ctttggcttg gctcagcctc ctcgccggcg cgctgctcgg cctcctcgcc gcgcggcaaa
      181 ccgcggcgga gcccgcgccc ggaccgcggt tttgggactg ggcggcgggg ctcctcttcg
      241 ccgccgtcag tctgcgttcc tttctctggc tgctctttga ggcgaatggc gagcttttca
      301 tccaatcccg ctacaatctc ggcgacctcg cgctgcatct gaatttcatc cagtatttcg
      361 ccagcggagc gcctttctgg ccggaaagcc cgatcttcca gggcgagccg ttgcgttatc
      421 ccatcggccc ggatttgttt aattccctgc tgctgctcgc cggcgttccg ctgcaacccg
      481 gcctcgtctg ggccgggctg atcggcgcgg ggctgaccgc ccatgcgctc tggcgctggg
      541 gcggcgcctt cggcgtggcc agcctgctca tggtcggcgg gctggccggc atctcgtttt
      601 ttcacaccgg gatactcgcc gactacctcg cccacatgga gtggaagaat ccattcctca
      661 gcctcttggt cccgcagcgc gcgtttttgt tcgcccttcc ggcgggactg atcctgctcc
      721 agcactggcg gcgcctgctc tccggggagc cgggccggct gccccgctgg gtggaggttc
      781 tgctctacgc ggcgatgccc ctgttcagcg tgcatacgtt tttgttcctg agcggcgcgc
      841 tgctgctgcg gttttggatc catcggaata gccgcgcttc ggcgctgcgg tgcgtggcca
      901 ttgccttcgt gccggccacg atcctcgtct ggctcgtcag cggcgggttc gggggaaaga
      961 gcttcatcgg ctggcatccc ggctggatgc agagcggcaa tccctgggcg ttctggttct
     1021 ggaatttcgg gctcatgatc ccgctcatgg ttggcgcggt gatcgttgcc acccgggagc
     1081 gcgaccgcgc cgccgctccg ttcgtctttg ccggcgcctt cttttttctg ctctgcgggt
     1141 ttgtgcgctt cagtccctgg ccctgggaca ataccaagct catgctctgg tcctgggtgg
     1201 ccatggcccc gttcatttgg aaaaagctgc tgctcccgct gccggtctgg ggccgcgcgg
     1261 gcgtgtgcgg agtgctcttt ttcaccggtt tcctctcgct gctcggcgcc atgggatcgg
     1321 aaaaccgctt cagcttcgcg cgggtggacg agctgcacgc ggcggaggcg gcggtccaag
     1381 cgattccgcc cgccgcgcgt tttgtcatca agccggacta caatcatccg ctgctgctgc
     1441 tcggccggcc cgtggtgtgc ggttacgacg ggcacctgtg gagccacggt tatgactacg
     1501 gcgccaaata cgaggccgcc gaagcggcga tgcgggggca aatctcgtgg ccggaagcgg
     1561 ccaaggcttt tgacttcgac tacgtatttt cgggaccgcg cgaggaagcc gcctacggac
     1621 agtcagccga accgatgccg caggtcattc caaagacgga gttgatcagc gacggcgggt
     1681 tcatcgaacc cgcgccgcct tgattgggca ggggacggct cgatgagccg tccgacttgt
     1741 ttcccgcctt cccaagcggg acaccgcttc agataaattc ctgccgcaaa aatcttctcc
     1801 ggccgaattc cagcgcggct ggcgccagaa agacaagcgg gacgaggaac ccggcagggt
     1861 gattaacaaa gaaaagaaag gccgattcca tgagctgcct ggcgtagatg ggaaaaatca
     1921 cgagaagcgc cagacagatg acaaaggcgc tccaatacca ccagcggcgg cggggacttg
     1981 gcaggatcag gaagatgaat gcctgccatc ccgtcaccgc atagaccaaa agagtgctgg
     2041 cccagagttt cagccaaaaa ccgccgagcg gaaccatcaa aaccgaattt ccggatttgc
     2101 cggcttgctg gaagaagctc cccggcagag cggtcagata gccgtccgcg gtttggggtt
     2161 tcacctcgag aacgaacgcg ggtttaaaaa aggccaaccc cagccagatg aaaatcggga
     2221 ccaggcacaa aagccaatac aaggcggtcc gagcccagta gatattcact cgatccagcg
     2281 cccgggtggc gaggaaatcg gaatgataca cctgaacctg caactgggtc gatgcgttca
     2341 gcgaaggcga cccggcaaaa atggccgaac cgaaaatgaa cgcggcgat
//
LOCUS       DATDAY010000140         2977 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_645273, whole genome shotgun sequence.
ACCESSION   DATDAY010000140 DATDAY010000000
VERSION     DATDAY010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2977)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2977)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2977
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_645273"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1330
                     /locus_tag="VIS74_02665"
     CDS             <1..1330
                     /locus_tag="VIS74_02665"
                     /inference="COORDINATES: protein motif:HMM:NF024371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3857 domain-containing protein"
                     /protein_id="HEY5792172.1"
                     /translation="PPPPHSTSTVSPDEIEIPKAEGDALFVAQPATEETRRILAEAGA
                     APNPLGYPAVLLRAENDLTMDATGATTEEETIRVFLKDTEALDRWASRSFPLDPPMIE
                     TKVLAARIILPDATACVLNPKKFSNACGGGVCGLTFPKAQAGSVVELSTIQSTRPAYE
                     LPMFYREFFLQEDIPTLSRRITLRLPKDKTYATYLKNAQAAPEASETEFSRVLTWKFG
                     PLAAFEPLPDSQPEREIVAWLGLSSVGSWEEFIRWYRHISEGAFEAGPKVQAKAKEIT
                     ATYPGRMERIRAAFEFVSALRYRFIELGIGGYRPRTPEQVMENLYGDCKDKANLLIAL
                     LRSMGIPADFVLINRLDSTDVAFPGWQFNHAIASVPPGEGQPETLWLDTTDTITPFGY
                     LAPGNFGRDALVFRDSDAVFLKVTSASTGPSRTLQRWALNETEPGRWNGT"
     gene            1340..1879
                     /locus_tag="VIS74_02670"
     CDS             1340..1879
                     /locus_tag="VIS74_02670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792173.1"
                     /translation="MTGLSDYFARLGLKGSPPLSRDYLLRQKLNSLWPEADFSKISIS
                     DVADIAVPLRLESRVSAAAARWPGIESELLRSVAAPERDRDLLLNDGQPLVIEQRVRL
                     ELAGAPVSRAGEVFEKQAAGQHFSIRYEWKDERTVERVALCEIRQPRISANAYPAFRA
                     AFRQWMAALDQPLPASKNL"
     gene            1876..2874
                     /locus_tag="VIS74_02675"
     CDS             1876..2874
                     /locus_tag="VIS74_02675"
                     /inference="COORDINATES: protein motif:HMM:NF040105.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY5792174.1"
                     /translation="MMTTLPPPDTTDLAPLQTLPETYQRLATAIRQRIIGQDALIRDI
                     FVALLSRGHCLMVGVPGLAKTLLVKTLGEIFDLSFHRIQFTPDLMPSDITGSEIIEQS
                     SDGRREFRFVPGPLFTQLLLADEINRTPPKTQAALLEAMQERQVTVAGKTYPLGAPFF
                     VLATQNPIEQEGTYPLPEAQLDRFMFCLHVGYPSLTEEIDILQRTTTNEEAAVPSVLA
                     ESEILSLQSAVRQIVVPHSVAEFAVRIVHASRPGNPLAPEAVKKYVRWGAGPRASQYL
                     ALAGKALAAMDGRFNVAVEDIVAAVPMVLRHRVLTNFRAEADGVEIEGILNLLVPR"
BASE COUNT          522 a         1088 c          853 g          514 t
ORIGIN      
        1 gccccccccg ccccactcca cctccacggt cagcccggat gaaatcgaaa tccccaaggc
       61 cgagggggat gcgcttttcg tggcccagcc cgccaccgag gaaacgcgcc ggattctggc
      121 cgaagccggg gccgctccca atcccctggg ttacccggcc gtgctcctgc gcgccgagaa
      181 cgacctcacg atggacgcca ccggcgccac gaccgaggag gaaacgatcc gggtcttcct
      241 caaggacacc gaagccctcg atcgctgggc cagccggtcc tttcccctgg atccgccgat
      301 gatcgaaacg aaggtgctgg ccgcgcgcat cattctgccc gacgccaccg cctgcgtcct
      361 caacccgaaa aaattcagca acgcctgcgg cggcggggtt tgcggcctca ccttccccaa
      421 ggcgcaggcc ggctcggtcg ttgaactgag cacgatccag agcacccggc ccgcctacga
      481 gctgccgatg ttttaccgcg aattctttct tcaggaggat atcccgaccc tcagccgccg
      541 gatcacgctc cgcctgccga aggacaaaac ctacgcgacg tatctgaaaa acgcccaggc
      601 tgcgccggag gcctcggaaa cggaattctc ccgggtgctg acctggaaat tcggcccgct
      661 ggccgccttt gagccgctgc ccgattccca gccagagcgc gagatcgtag cctggctggg
      721 cctcagttca gttggctcct gggaggaatt tatccgctgg tatcgccaca tttccgaggg
      781 cgccttcgag gccggcccga aggtccaggc gaaggccaag gaaatcacgg ccacctatcc
      841 gggccgaatg gaacgcatcc gcgccgcctt cgagttcgtt tccgccctgc gctatcgttt
      901 catcgaactc ggcatcggcg gttatcggcc gcgcacgccg gaacaggtca tggaaaatct
      961 ctacggcgac tgcaaggaca aggccaatct cctgatcgcc ctgcttcggt ccatgggaat
     1021 cccggcggat tttgtgctga tcaaccgcct ggattcgacc gacgtggctt tccccggctg
     1081 gcagttcaat cacgccattg ccagcgtgcc gcccggcgag ggccaaccgg agaccctctg
     1141 gctcgatacc acggatacca tcacgccctt cggctatctg gctcccggca acttcggccg
     1201 cgacgcgctg gtcttccggg attcggacgc cgtcttcctg aaagtcacct ccgcttccac
     1261 cggacccagc cgcaccctcc agcgatgggc cctgaacgaa accgaaccgg gccggtggaa
     1321 cgggacttag tccgagagct tgaccggcct ctccgattat tttgcgcggc tgggcctcaa
     1381 gggcagcccg cccttgagcc gggattatct gctccggcaa aaattgaatt cgctctggcc
     1441 ggaagcggat ttttccaaaa tctcgatctc cgatgtggcc gacatcgccg tgccattgcg
     1501 cctggaaagc cgggtttcgg ccgcggccgc gcgctggccc ggcatcgaat ccgagctgct
     1561 ccgcagcgtc gccgctcccg aacgggaccg ggatctcctc ttaaatgacg gccagccgct
     1621 cgtcatcgag cagcgcgtgc ggctggagct ggccggcgct ccggtttccc gggccggcga
     1681 ggtttttgag aaacaagccg ccggccagca tttctccatc cgctacgaat ggaaggacga
     1741 gcgcaccgtc gagcgcgtgg ccctttgcga aatccgccag ccgcgcattt ccgccaacgc
     1801 gtatcccgcc ttccgcgctg ccttccgcca atggatggcc gccctcgatc aacccctgcc
     1861 cgcttccaaa aatctatgat gaccaccctg ccgccgccgg acaccaccga cctcgctccg
     1921 ctgcaaaccc tgcccgagac ctatcagcgc ctggccaccg ccatccgcca gcgcatcatc
     1981 ggccaggatg ccctgatccg cgacattttc gtcgccctgc tctcccgcgg gcactgcctc
     2041 atggtcggcg tccccggact ggccaagacg ctgctggtca agacgctggg ggaaatcttc
     2101 gacctcagct ttcaccgcat tcaattcacc cccgacctga tgcccagcga catcaccggc
     2161 tcggagatca tcgaacaaag ctccgatgga cgccgcgagt tccgcttcgt gcctggcccg
     2221 ctgttcacgc agctgctgct ggcggacgaa atcaaccgca cgccgcccaa gacgcaggcc
     2281 gccctgctcg aagccatgca ggaacgccag gtgaccgtcg cgggcaagac ttatccgctg
     2341 ggcgccccct tcttcgtgct ggccacgcag aaccccatcg aacaggaagg cacctatccg
     2401 ttgcccgagg cgcagttgga ccgcttcatg ttctgcctgc acgtcggtta tccgtccctc
     2461 acggaggaaa tcgacattct gcaacgcacc accaccaacg aggaggcggc cgtgcccagc
     2521 gtgctggccg aatccgaaat cctctccctg caaagcgctg tgcgccagat cgtcgtcccg
     2581 cacagcgtgg cggaattcgc cgtccggatc gtccacgcct cccgcccggg caatccgctc
     2641 gcccccgagg cggtgaaaaa atacgtgcgc tggggcgccg ggccgcgcgc ctcgcaatac
     2701 ctcgccctgg ccggaaaggc gctggcggcc atggatggac gcttcaacgt ggccgtggag
     2761 gacattgtcg cggccgttcc catggtgctg cggcaccggg tgctgaccaa tttccgcgcc
     2821 gaggccgatg gggtcgagat tgagggcatc ctgaacctgc tcgttccccg ctagattttc
     2881 cacggacatg agcgcccggc gccccatcgc ggattaccag ccccctccga gcgagccgct
     2941 cgatctcgcc ctgctggccg cgcttcccaa tctgccg
//
LOCUS       DATDAY010000141         2901 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_1445219, whole genome shotgun sequence.
ACCESSION   DATDAY010000141 DATDAY010000000
VERSION     DATDAY010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2901)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2901)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2901
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1445219"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..799
                     /locus_tag="VIS74_02680"
     CDS             <1..799
                     /locus_tag="VIS74_02680"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY5792175.1"
                     /translation="IIGSRVAVNYRDAGHPVWLWNRSPKPLPGFLGSPQEVARRAARI
                     FIYVADGPASLAVIDQMAPALTPGHVIVNHATIGPDEARECARRAAAAGARYLDAPFT
                     GSRDAAAQRQIVYYLGGDEAVQRAVEPILRLNAKAILPVGGVGDASLMKLATNLVSAS
                     IVSALAEAVAVVRAAGGDPEKFALALENNASRSATTDLKLPAMLRQDFAPRFSLKHML
                     KDTRLVRGLAASHGLPLPSTEAFIAKAAALAERGFGDEDFSVLGKTE"
     gene            796..1617
                     /locus_tag="VIS74_02685"
     CDS             796..1617
                     /locus_tag="VIS74_02685"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792176.1"
                     /translation="MKPACVEILDRARVRVSGADAARYLNGQLSNDLRKLPPGAALRA
                     CLLTAKGKLVADPFVWREGEDFILEAHASLQEALLARLERYIVADDVTVEPLEVVPAT
                     YHVLGVAEPAGRAINRLGVPGFDAAEPPAGIPRLDFADAETIRIRHAMPLWGKELDAD
                     VLPAEAGLDVLAVDFHKGCYVGQEVVSRMETAGRTRRQLAVLQTRPLLPEGTELFAPG
                     GEHAIGRLTSSTPEAGGGVALAWLTPAFAAAGTRLKPAAGESDSLTDIEVIEKLP"
     gene            1619..2251
                     /locus_tag="VIS74_02690"
     CDS             1619..2251
                     /locus_tag="VIS74_02690"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEY5792177.1"
                     /translation="MRFLTLLAILFSTATALHAQLGPDVAVFQIQFGRDKALQQVVIG
                     LYDSATPATVDNFKTLIAEKYYNGMRFHRVFPNSMVQTGDPLSKRGPDDRSGTGGPGY
                     TVPAEIRKPHARGSVAMSRISGPSNPAKASSGSQFYVCLQPLPKLNGQYTVFGEVLQG
                     LDVLEKISNARTDTNDFPVDKIVIRKVTLEPRGVFEAAPAPAAPLPQTPN"
     gene            2325..2410
                     /locus_tag="VIS74_02695"
     tRNA            2325..2410
                     /locus_tag="VIS74_02695"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2359..2361,aa:Tyr,seq:gta)
BASE COUNT          509 a         1037 c          866 g          489 t
ORIGIN      
        1 aatcatcggc agccgcgtcg cggtgaatta ccgcgacgcc ggccatcccg tctggctttg
       61 gaaccgctcg cccaagccgc tcccgggctt cctgggctcc ccgcaggagg tcgcccgccg
      121 cgccgcgcgg attttcatct acgtggcgga tggcccggcc agcctggccg tcattgacca
      181 gatggccccg gccctcacgc ccgggcacgt gatcgtgaac catgccacga tcggcccgga
      241 cgaggcgcgc gaatgcgccc ggcgcgccgc cgccgccggc gcgcggtatc tcgatgcgcc
      301 cttcaccggc agccgcgacg ccgcggcgca gcgccagatc gtctattacc tcggcggaga
      361 cgaggcggtc caacgcgcgg tcgaaccgat tctccgcctc aatgcgaaag ccattctgcc
      421 cgtcggcggg gtcggcgacg ccagcctgat gaagctggcc accaacctgg tcagcgccag
      481 cattgtctcc gcgctcgccg aggcggtcgc cgttgtccgg gcggccggcg gcgatccgga
      541 aaaattcgcg ctcgcgctgg aaaacaacgc ctcccgctcc gccacgaccg atctcaagct
      601 gcccgccatg ctgcggcagg atttcgcccc ccgcttttcg ctcaaacaca tgctgaagga
      661 cacgcggctg gtccgcggcc tcgccgccag ccacggcctc ccgctgccct ccaccgaggc
      721 gttcatcgcc aaagccgccg cgctggccga gcggggattc ggcgacgagg acttttccgt
      781 cctgggaaaa acggaatgaa acccgcctgc gtcgaaattt tggatcgcgc ccgggtccgg
      841 gtctccggcg cggacgccgc gcgttatttg aacgggcagc tttccaacga cctgcggaaa
      901 cttccgcccg gcgccgccct gcgcgcctgc ctcttgaccg ccaagggcaa gctggtcgcc
      961 gatccctttg tctggcgcga gggcgaggat ttcatcctcg aagcgcacgc ctccctccag
     1021 gaggcgctcc tggcccggct ggagcgttac attgtcgccg acgacgtcac cgtggagccg
     1081 ctggaagtcg ttcccgcgac ctatcacgtc ctcggcgttg ccgaaccggc cggccgcgcc
     1141 atcaaccggc tcggcgtgcc gggattcgat gccgccgaac cacccgcggg gattccgcgg
     1201 ctggatttcg cggatgcgga aaccatccgc atccggcacg ccatgccgct gtggggcaag
     1261 gaactcgatg cggacgtgct gcccgccgag gcggggctcg atgtcctcgc cgtggatttt
     1321 cacaagggct gctacgtcgg ccaggaagtc gtttcccgca tggagaccgc cggacgcacc
     1381 cgccgccagc tcgcggtcct ccaaacccgg cccctcctcc cggaaggaac cgaattgttc
     1441 gcgcccggcg gcgaacacgc gatcggccgc ctcacctcat ccaccccgga agccgggggc
     1501 ggcgtggcgc tggcctggct caccccggcg ttcgcggccg ccggaacccg cctaaaaccg
     1561 gctgccggtg aaagcgatag cttgacggac atcgaagtca tcgaaaaact cccgtgacat
     1621 gcgatttctg acgcttctcg ccattttatt ttcgaccgcc accgcgttgc acgcgcagct
     1681 cggcccggat gtcgccgttt tccaaatcca gttcggacgc gacaaggcgc tgcagcaagt
     1741 cgtgatcggc ctctacgatt cggcaacgcc ggcgacggtg gacaacttca aaacgctgat
     1801 cgccgaaaaa tattacaacg gcatgcggtt ccatcgcgtg tttcccaatt cgatggtgca
     1861 aaccggggat ccgctcagca agcgcggccc cgacgaccgc tccggcaccg gcggccccgg
     1921 ttacaccgtg cccgcggaga tccgcaagcc gcacgcccgc ggctccgtcg ccatgtcccg
     1981 catctccggc ccgtccaatc cggccaaggc cagcagcggc agccaattct acgtctgcct
     2041 gcagccgctg cccaagctga acgggcaata caccgttttc ggtgaagtgc tgcaagggct
     2101 cgacgtgctg gaaaaaatca gcaacgcgcg cacggacacc aacgatttcc ccgtggataa
     2161 gatcgtgatc cggaaagtca cgctcgagcc gcgcggggtt ttcgaggcgg ctcccgcgcc
     2221 cgcggcgccc ctgccgcaga cgccgaatta agaaagcccg aacttttctc tggaaatttt
     2281 ggcggagtcg ttcattcttt cccccctttc cccatcacct ttacgggtag gtaccgaagt
     2341 ggccaaacgg ggcggactgt aaatccgctg gcttacgcct tcactggttc gaatccagtc
     2401 ctgcccacca cttccgctag cgcggaagag cgaggagtga aggggcgggg ctaaagggaa
     2461 aagtcgctcc cggactgggt tcgaacgccg ttcgaccgcc ccgcgcgaag cgcaatttga
     2521 atcccgcgaa gccttgatgc ggagccccgc tgcggcgggg ataatccagt cctgcccacc
     2581 acttccgcta gcgcggaaga gtgaaagggc cggagccaaa gggaaaagtt gctcgccaga
     2641 ccgggtttga acgccgcgcg cgaagtgcga ttcgaatccc gcaaaaccat gggaccgcct
     2701 ttgggaccga ggccttctca tttcccattc ttcacttgct tcccgctctt ccttcacttt
     2761 gatgatggaa acgcaaccca tgaatccacc ccaatcgacc cctccttatt cgaacaaacg
     2821 cggccgactt ttgcccgcaa tcctcctcgc gggcataatg atcggcatga atctacaggc
     2881 ggcggcgctg gcgccgattc c
//
LOCUS       DATDAY010000142         4330 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_324150, whole genome shotgun sequence.
ACCESSION   DATDAY010000142 DATDAY010000000
VERSION     DATDAY010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 4330)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4330)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4330
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_324150"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..885
                     /locus_tag="VIS74_02700"
     CDS             <1..885
                     /locus_tag="VIS74_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HEY5792178.1"
                     /translation="ILERTREHCFRSLHDARLEPEELQEVILVGGATRMPLVRRLAAE
                     WFGREPNLSQHPDEAVALGAVIQAGILEGALRHVTLLDVTPLSLGLETFGGLMNVLIP
                     RNTTIPCKAGEMFTNAVANQRSMRITVMQGEREIARDNFQLGEFEIPFTPAPKGQARV
                     GVQFEIDANGLLKVLARDIATGQDKIVEIHSAVDVAGTEVERMISDSVDHAFDDMAER
                     IFIETKLKAEELLAAVEAARLQIELTEAEEAQIAALQAEVKNALQTRETQRLKKAVAE
                     LDDLTEPLAARILEKLMG"
     gene            1017..1535
                     /locus_tag="VIS74_02705"
     CDS             1017..1535
                     /locus_tag="VIS74_02705"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY5792179.1"
                     /translation="MSDDEFFQQTLARYERPLISYAKSITGDWESAKDAVQETFLRLS
                     RENLKTLEPRLAAWLFAVCRTRAIDYQRKIVRFVSSEDGALESTPADGPEPPAQLVQA
                     EDRAQLSELLARLPENQREVVKLKFEAGLSYREISEVTRHSVSNVGFLLHTAIQTLRR
                     QWNQTTKLPTLS"
     gene            1532..3811
                     /locus_tag="VIS74_02710"
     CDS             1532..3811
                     /locus_tag="VIS74_02710"
                     /inference="COORDINATES: protein motif:HMM:NF023459.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="von Willebrand factor type A domain-containing
                     protein"
                     /protein_id="HEY5792180.1"
                     /translation="MKTEDIRITQYTLGELAGAEREAFEKEMAADPGLLEEVRLTQQF
                     CESLPPNPESAAAFSEAERQALMAKWTEARAERGGRKVWPRVFLYGGLAATAACVVAS
                     LSIPSVTGALTRRGPEMAKIETREREFNSLSVAQAAATPASLPAETSGLVAANERMLQ
                     APLEESKNSADSPAMLEKSEQAQMLAAAPSAGQPQAPADERQVLQEALPYLATAAPAV
                     TVNRKSLDGNMFTVTESAGAAPLAEIQTLSVSGSGVAGFAGKAKDRLARVEFDHSRAK
                     KAEAFNTEGYDYLEENRFLSARENPLSTFSIDVDTASYSNVRRFLQQQGQLPPKGAVR
                     IEELVNYFPYSYPQPEGTDPFSVNVEVGKAPWNPRHDLVRIALKGREIAAGKRPASNL
                     VFLVDVSGSMDEPDKLPLLQRSLVELTLRLAPKDRVAIVVYAGSSGLVLPSTADKAAI
                     RDALERLQAGGSTNGGEGIKLAYREARANFLEDGNNRVILCTDGDFNVGVTSEQALVD
                     LIEKERASGVFLSVLGFGTGNLKDSQMEKLADRGNGNYAYIDSPAEGRKVLGEQMGAT
                     LFTIAKDVKIQVEFNPAAVEGYRLIGYENRLLAKEDFNDDKKDAGEIGSGHTVTAFYE
                     VVPAGQPLPDAASVDELKYQPVDSKNPQSAIRNPQSNELLTVKLRYKQPDGGTSQLLQ
                     APVANQAKEWNACSEDFRFGAAVAAFGLKLRHSKTVKAVDWAQIQKLARTSLGEDPGS
                     YRAEFLTLIEKARQIDENP"
     gene            3863..>4330
                     /locus_tag="VIS74_02715"
     CDS             3863..>4330
                     /locus_tag="VIS74_02715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792181.1"
                     /translation="MSQTLLPANSIARWLLILILLAGAYFFYGFLIPVLAALIVGFAS
                     WPLYRRLVARCRGRTALAATAALLIVLLVVLTPLTWFLVYSAQEINSGVSWLMEANRQ
                     GMPVPDWIRALPVVGPRAAAQWAEFLSPPNGLSHTVQMVSGENIEKIYRWVVGA"
BASE COUNT          867 a         1380 c         1359 g          724 t
ORIGIN      
        1 atcctcgaac gcacgcgcga gcattgcttc cgttccttgc acgacgcacg gctggagccc
       61 gaggagttgc aggaagtcat tctcgtcggc ggcgccacgc gcatgccgct ggtccgccgg
      121 ctcgccgcgg agtggtttgg ccgcgagccg aatctcagcc agcatcccga cgaagcggtc
      181 gcactgggcg cggtgatcca ggccggcatc ctcgagggcg cgctgcgcca tgtcacgctg
      241 ctcgacgtca ccccgctttc gctgggcctg gaaaccttcg gcggcctcat gaatgtgctc
      301 atcccgcgga atacgacgat tccctgcaag gccggcgaga tgttcaccaa cgccgtcgcc
      361 aaccagcgga gcatgcgcat cacggtgatg cagggggagc gcgagatcgc ccgggacaat
      421 ttccagctcg gcgaattcga aattcccttc acgcccgcgc cgaagggcca ggcgcgggtc
      481 ggcgtgcagt tcgagatcga tgccaacggc ctgctcaagg tgctcgcgcg cgacatcgcc
      541 accggccagg acaaaatcgt cgaaatccac agcgccgtgg acgtggccgg caccgaggtc
      601 gagcgaatga tctccgactc cgtggatcac gccttcgacg acatggccga gcggatcttc
      661 attgaaacga agctgaaggc cgaagagctg ctggccgccg tggaggccgc gcggttgcag
      721 atcgaattga ccgaggcgga ggaggcgcaa atcgccgcgc tccaggccga ggtaaaaaac
      781 gctctccaga cccgcgaaac ccaacgattg aaaaaagctg tcgccgagct ggacgacctt
      841 accgaacccc tggccgcccg gattctcgaa aaactgatgg gataaatctg aacagaagaa
      901 agcaaaggaa gggaagaggg caatgccgga ttttcccctt tgcttgcttt gccgccttct
      961 gttcaaaaaa tttccctaat aaactttggc cccgcccgtt gactcattga aaccccatga
     1021 gtgacgacga atttttccaa caaacgctgg cgcgatatga gcggccgctc atcagctacg
     1081 cgaaatccat tacgggcgac tgggagagcg cgaaggacgc cgtgcaggaa accttcctcc
     1141 ggctcagccg cgaaaatctc aagaccctcg agccacgcct cgccgcgtgg ctttttgccg
     1201 tctgtcgaac gcgggcgatc gactatcaac gcaaaatcgt gcggttcgtg tcgtccgagg
     1261 acggcgcgct ggagtccacc ccggcggacg ggcccgagcc gcccgcgcag ctggtccagg
     1321 cggaggaccg ggcgcaattg tccgaactgc tcgcgcgcct gccggaaaac cagcgcgaag
     1381 tcgtcaaact caaatttgaa gcgggcctga gctaccgcga aatcagcgaa gtcacgcggc
     1441 acagcgtcag caacgtcggc ttcctcctcc ataccgccat ccaaaccctg cggcggcaat
     1501 ggaaccaaac caccaaactc cccaccttgt catgaagacg gaagatattc gcatcaccca
     1561 atacaccctc ggcgaactgg ccggggccga gcgcgaggcg ttcgaaaaag aaatggccgc
     1621 cgatcccggg ttgctggagg aagtccggct gacccagcag ttctgtgaat ccctgccgcc
     1681 gaatccggag tccgcggcgg ccttttcgga ggcggagcgg caggctttga tggccaaatg
     1741 gaccgaagcg cgggccgaac gcggcggccg caaggtctgg ccgcgggttt tcctctacgg
     1801 cggcctggcc gccacggcgg cgtgcgtggt ggcgagcctt tccattccct cggtcacggg
     1861 cgcgctcacg cgccgcggtc cggaaatggc gaaaatcgaa acgcgcgagc gggaattcaa
     1921 ttccctctcc gtggcccagg ctgccgccac gccggcatcg cttcccgccg agacttccgg
     1981 gttggtggcg gccaacgaga ggatgcttca ggcccccttg gaagagtcca aaaacagcgc
     2041 agactcgccc gcgatgctgg agaaatccga gcaggctcaa atgctggcgg cggctccgtc
     2101 cgccgggcag ccccaagccc cagcggatga aaggcaggtg ctccaggaag cgctccctta
     2161 cctcgccacg gccgcgccgg cggtgaccgt gaaccgcaag tcattggatg gtaacatgtt
     2221 cacagtcacg gagtcggccg gcgcagcccc gttggcggaa atccaaaccc tctccgtcag
     2281 tggaagcggg gtggcgggat tcgcgggcaa ggccaaggat cgcctcgcga gggtggaatt
     2341 cgaccattcc agggcgaaga aagccgaagc cttcaacacc gagggttacg actacctgga
     2401 ggaaaatcgg ttcctcagcg cccgggaaaa tccgctttcg acgttttcga tcgacgtgga
     2461 caccgcgagc tacagcaacg tccggcgttt ccttcagcag cagggccagc tcccgccgaa
     2521 gggggcggtg cggatcgagg aactggtgaa ctattttcct tactcctacc cgcaacccga
     2581 ggggacggat cccttttccg tcaatgtcga ggtcggcaag gcgccgtgga atccgcggca
     2641 cgatctcgtg cgcatcgccc tcaagggccg ggaaatcgcc gccggcaagc gccccgccag
     2701 caacctcgtg ttcctcgtcg atgtctccgg ctcgatggac gaacccgaca agctgccgct
     2761 cctacagcgt tccctggtgg agctcacctt gcggctggcc ccgaaggatc gcgtcgccat
     2821 cgtcgtctat gcgggatcgt ccggattggt gctgccttcc acggcggaca aggccgccat
     2881 tcgcgacgcg ctggagcgtc tccaggccgg cggctcgacc aacgggggcg aaggcatcaa
     2941 gctggcctac cgcgaggcgc gggcgaattt cctcgaagac ggcaataacc gcgtgatcct
     3001 ctgcaccgac ggcgatttta atgtcggcgt caccagcgag caggcgctcg tggacctcat
     3061 cgaaaaagag cgcgcgtccg gcgtctttct ctccgtgctg ggcttcggca cgggcaacct
     3121 caaggacagc cagatggaaa aactcgctga caggggcaac ggcaactacg cctacatcga
     3181 ctcgcccgcc gaaggccgca aggtgctcgg cgagcagatg ggcgcgacgc ttttcaccat
     3241 cgccaaagac gtgaaaatcc aggtggaatt taatcccgcc gcggtcgagg gctatcgcct
     3301 gatcggctac gaaaaccgcc tgctggcgaa ggaggatttc aacgacgaca aaaaggacgc
     3361 cggcgagatt ggctccggcc acaccgtgac cgcgttctac gaggtcgtgc ccgccgggca
     3421 gcccctgcca gacgcggctt cggtggatga attgaaatac cagcccgtgg attccaaaaa
     3481 tccgcaatcc gcaatccgca atccgcaatc gaatgagctg ctcaccgtga agctgcgcta
     3541 caaacagccg gatggcggca cgagccagct cctccaggcg cccgtagcca atcaggcgaa
     3601 ggaatggaac gcgtgctcgg aggatttccg cttcggcgcg gcggtggcgg cctttggcct
     3661 gaaactgcgg cactcgaaaa cggtgaaagc cgtggactgg gcgcagatcc agaagctcgc
     3721 ccggacgagc ctcggggaag atccgggcag ctaccgcgcc gagttcctga cgttgatcga
     3781 aaaagcccgg cagattgacg aaaatccata gacctccgcc ggaattgctg gaataagggg
     3841 tgtcggtatt gccgatgccc ccatgtctca aacgctgctg cctgcaaatt caatcgcccg
     3901 ctggctgttg attctcatat tgctcgcggg agcgtatttt ttttacgggt ttttgattcc
     3961 ggtgctcgcg gcattgatcg tgggattcgc gagctggccg ctgtatcggc ggctggtggc
     4021 gcgctgccgc ggccgcaccg ccctggcggc gacggccgcc ctgctcatcg tgctgctggt
     4081 ggtgctgacg ccgctgacgt ggtttttggt ttactcggcg caggagatca actcgggcgt
     4141 atcctggctg atggaagcca accgccaggg gatgccggtg ccggattgga ttcgcgccct
     4201 cccggtggtc ggcccgcggg cggcggcgca atgggcggaa ttcctgagcc cgccgaacgg
     4261 cctgagccac acggtgcaaa tggtcagcgg cgagaatatc gagaaaatct accgctgggt
     4321 cgtcggcgcc
//
LOCUS       DATDAY010000143         3680 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_1396208, whole genome shotgun sequence.
ACCESSION   DATDAY010000143 DATDAY010000000
VERSION     DATDAY010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 3680)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3680)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3680
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1396208"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..755
                     /locus_tag="VIS74_02720"
     CDS             <1..755
                     /locus_tag="VIS74_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sulfite reductase subunit alpha"
                     /protein_id="HEY5792182.1"
                     /translation="AELDKYIWGRGVIDILTEFDAVFTPEEFVKALRKLQPRLYSIAS
                     SPRAVGEEVHLTVAIVRYESHGRRREGVCSSFLADRAGGGKVPVFVHSAKHFRVPENP
                     ETDVIMVGPGTGIAPFRAFLQERKAAGAKGRNWLFFGDQRSSTDFLYEDEFKAYQAEG
                     ILTKFDTAFSRDQEYKIYVQHRMAEHAAELYAWLEGGAYFYVCGDANRMAKDVDVALH
                     QAVQTAGGKTEEGAKEYVAKLKTEKRYRRDVY"
     gene            845..2236
                     /gene="mpl"
                     /locus_tag="VIS74_02725"
     CDS             845..2236
                     /gene="mpl"
                     /locus_tag="VIS74_02725"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HEY5792183.1"
                     /translation="MSAISSPKHVHFLGICGTAMGAVAAGMKELGFEVTGSDENVYPP
                     MSTFLESKGIRLMAGYRAENLPESADLFVVGNTIRRGNPEVEALLNRKWPYLSLPETL
                     KFFFLRGRHNLVVTGTHGKTTTTSLLTWIFESAGRNPSWLIGGIPHNLGQGCCLRESK
                     HFILEGDEYDTAFFDKRSKFVHYLPELVIVNNLEFDHADIFNNLEEIKTSFRRLLNIV
                     PNEGMVLLNADDPNCVDLARTCPAPILEVGFSPNAGNQIRHVSYGADGSRFEVFGETF
                     EVPLLGEFNVRNAAMAASAAHFYGITPAEIRKAFAEFKGIKRRQEIRGEVRGIKVIDD
                     FGHHPTAIKQTLQGLRARYADARLWAIFEPRSNTTRRAVFQHQLPQAFELADGVLIAQ
                     VARLEQLAPEERLNPEKVVADIAAQGKPAFYEPTADDIVEKLKPLAKAGDVVVVFSNG
                     GFDGIHQKLLERL"
     gene            complement(2304..3248)
                     /locus_tag="VIS74_02730"
     CDS             complement(2304..3248)
                     /locus_tag="VIS74_02730"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM domain-containing protein"
                     /protein_id="HEY5792184.1"
                     /translation="MIWFAIIACIIIAFLFSGIESGVVSINRVRLRHNAGLGAPGARE
                     LEALLAHPGRLIVTVTLVTTGSKVLALSLLFSVFARHFGHLGEAAALVACLPVFAVLV
                     EVIPKAIFSRFPYRTLVFFARILQIANWLVYPISALAEWIARPLVHASQETHARHITS
                     VEGIRRAVNESTSKSALQPLQKHLMHSVLNFREIRAGKLMLPIEKVVSVTGESWVSDA
                     LDLARQTQIDRLPVLNANGEVTGLLQIFDLLIDGVKSGRVQSYARRVIAVRPDEPAIE
                     VLIKLRAARLAMAVVTDANGKALGILPAEDLVRKMLLG"
     gene            complement(3245..>3680)
                     /locus_tag="VIS74_02735"
     CDS             complement(3245..>3680)
                     /locus_tag="VIS74_02735"
                     /inference="COORDINATES: protein motif:HMM:NF015436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transporter associated domain-containing
                     protein"
                     /protein_id="HEY5792185.1"
                     /translation="TMNALELLRNFLNRKQALAILLDEYGGVAGVVTLSDLLEELLGE
                     EGASAQSELYIERIHADRLLASGNARLDDLGEFLGLDFKDENADTLGGLIVERLGQLP
                     KPGASLKIDGWLITVRRVTRKRIRETLIERPAKESSTPEAAP"
BASE COUNT          712 a         1091 c         1188 g          689 t
ORIGIN      
        1 aggccgagct cgacaaatac atctggggac gcggggtcat cgatatcctc acggaattcg
       61 acgcggtttt cacgcccgag gaattcgtca aggcgctccg caagctgcag ccgcggcttt
      121 attcaatcgc ctcctcgccc cgggcggtgg gcgaggaagt gcatctgacg gtggcgatcg
      181 tgcgctacga aagccacggc cgcaggcgcg agggcgtctg ctcgtccttc ctcgcggacc
      241 gcgcgggcgg gggcaaggtg cccgtatttg tgcactccgc gaagcatttc cgcgtgccgg
      301 aaaatccgga gacggacgtc atcatggtcg gtcccggcac cggcatcgcg cccttccgcg
      361 ccttcctgca ggaacgcaag gccgccggcg ccaagggccg caactggctg ttcttcggcg
      421 atcagcgttc gtccacggat ttcctctacg aggacgagtt caaggcgtat caggccgagg
      481 gaatcctgac gaaattcgac accgcgtttt cacgcgatca ggaatacaag atttacgtcc
      541 agcaccgcat ggccgagcac gccgcggaac tgtatgcctg gctcgaaggc ggcgcctatt
      601 tctacgtctg cggcgatgcc aaccgcatgg cgaaggacgt ggatgtcgcg ctgcatcagg
      661 ccgttcagac ggcgggcggc aagaccgagg aaggggcgaa ggaatacgtc gccaagctca
      721 agaccgaaaa gcgctaccgc cgcgacgttt actgagattt tccgacagaa ttaacggaat
      781 ttacaaaatt ccttgggcag gaaattctgt ccattctgtt aattctgtct aaatcccttc
      841 cgccatgtcc gccatttctt ctcccaagca cgttcatttt ctcggcatct gcggcaccgc
      901 catgggcgcg gtggcggcgg ggatgaagga gcttggattt gaggtcaccg ggtcggatga
      961 aaatgtgtat ccgccgatgt ccacgttcct ggagagcaag ggcattcgtt tgatggccgg
     1021 ttaccgcgcc gaaaacctgc cggagtccgc ggatttgttc gtggttggca acacgattcg
     1081 ccgcggcaac ccggaggtgg aggcgctgct gaaccgcaag tggccttatt tgtcgctgcc
     1141 cgagacgctc aagtttttct ttttgcgcgg aaggcacaat ctcgtggtga ccggaaccca
     1201 tggcaaaacg acgacgacct ccttgctgac gtggatcttt gaaagcgccg gccgaaatcc
     1261 cagctggctc attggcggca ttccgcacaa tctgggccag ggctgctgcc tgcgcgaatc
     1321 gaagcatttc atcctcgaag gcgacgaata cgacaccgcg tttttcgaca agcgctccaa
     1381 gttcgtccat tacctgcccg agctggtgat cgtgaacaat ctggaattcg accacgccga
     1441 catcttcaac aacctcgagg aaatcaagac gagcttccgc cggctgctga acatcgttcc
     1501 gaacgagggc atggtgctgc tcaatgcgga tgatcccaat tgcgtggacc tcgcccgcac
     1561 ctgccccgcg ccgattctcg aagtgggttt ctcgccgaac gcgggcaatc agatccgcca
     1621 tgtcagctac ggagcggatg gctcgcgttt cgaggtgttc ggcgagacgt tcgaggtgcc
     1681 gcttttgggt gaatttaacg tccgcaatgc ggccatggcc gcctcggcgg cgcattttta
     1741 cggcatcacg ccggcggaga ttcggaaggc ctttgcggaa ttcaagggca tcaaacgccg
     1801 gcaggaaatt cgcggcgagg tgcgcggcat caaggtgatc gacgactttg gccatcatcc
     1861 gaccgccatc aaacaaacgc tccagggcct gcgcgcccgt tacgccgacg cccggctgtg
     1921 ggcgattttt gagccgcggt ccaataccac gcgccgcgcg gtgtttcagc accagttgcc
     1981 gcaggcgttc gaactcgcgg atggcgtgct gatcgcccag gtggcccggc tggagcagct
     2041 cgcgccggag gagcggctga atcccgaaaa agtcgtcgcg gacatcgccg cgcagggcaa
     2101 accggcgttt tacgagccga cggcggacga catcgtggaa aagctcaagc cgctggcgaa
     2161 ggcgggcgac gtggtggtcg ttttcagcaa cggcgggttc gacggcatcc accagaagct
     2221 gctggagcgg ctgtaaggaa ttgcggattg cggatttggg agtgcggaat ggagtggcgc
     2281 gggggtaatc cgcatcgaac gtttcacccc agcagcattt tccgcacgag atcctcggcg
     2341 gggaggatgc ccagggcctt gccattggca tccgtcacca ccgccatggc gaggcgggcg
     2401 gcgcgcagct tgatgaggac ttcgatggcg ggttcgtccg gccgcacggc gatcacgcgc
     2461 cgggcgtagg actggacgcg gccggacttc acgccatcga tgagcagatc gaaaatctgc
     2521 agcagtccgg tcacctcgcc attggcgttg agcacgggca ggcggtcgat ctgggtctgg
     2581 cgcgcgaggt cgagcgcgtc gctcacccag ctttcacccg tcacggagac cactttttcg
     2641 attggcagca tcaatttccc ggcgcggatt tcccggaaat tcagcacgga gtgcatgagg
     2701 tgcttttgca gcggctgaag ggcgcttttg ctcgtggatt cgttgaccgc gcggcggatg
     2761 ccttccaccg acgtgatgtg gcgcgcgtgc gtttcctgcg aggcgtgaac cagcgggcgc
     2821 gcgatccatt ccgccaaggc ggagatggga tagaccagcc agttggcaat ctgcaggatc
     2881 cgcgcgaaga aaaccagcgt gcgatacgga aagcggctga agatcgcctt cggaatcact
     2941 tcgacgagca ccgcgaatac cggcaggcag gcgacgaggg cggccgcttc gcccaggtgg
     3001 ccaaaatgcc gggcaaacac gctaaagagc agcgacagcg ccagcacctt ggagccggtg
     3061 gtgacaagcg tgaccgtgac gatgagccgg cccggatgcg ccagcagcgc ctccagttcc
     3121 cgggcgccgg gggcgccgag ccccgcgttg tggcgcagcc ggacccggtt gatggaaacc
     3181 actcccgatt cgattcccga aaagaggaag gcgatgatga tgcaggcaat gatggcgaac
     3241 cagatcatgg ggcggcctcc ggggttgagg attccttcgc cgggcgttcg atcagggttt
     3301 cgcggatgcg tttgcgggtg acgcgccgca cggtgatcag ccagccgtcg attttcagcg
     3361 aggcgccggg cttgggcaat tggccaaggc gctccacgat caatccgccg agcgtatcgg
     3421 cattttcgtc cttgaaatcg aggccgagga attcgcccag atcgtcgagg cgggcattgc
     3481 cgctggcgag caggcgatcg gcatggatgc gttcgatgta gagctcgctt tgcgcgctgg
     3541 cgccctcctc gccgaggagc tcctccagca aatcagacaa tgtgaccacg ccggcgacgc
     3601 cgccatactc gtcgagaaga atggcgagcg cctgcttgcg gttcagaaaa tttcggagca
     3661 gttccaatgc gttcatggtt
//
LOCUS       DATDAY010000144         3193 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_1145045, whole genome shotgun sequence.
ACCESSION   DATDAY010000144 DATDAY010000000
VERSION     DATDAY010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 3193)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3193)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3193
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1145045"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..200)
                     /locus_tag="VIS74_02740"
     CDS             complement(<1..200)
                     /locus_tag="VIS74_02740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792186.1"
                     /translation="MTVLAIEDPAAVNGMPTVSSLWKLLHHFDCDNVMAAPTGTVSAK
                     EDSAATPSRKTRQCRRPQTSNAR"
     gene            417..1367
                     /locus_tag="VIS74_02745"
     CDS             417..1367
                     /locus_tag="VIS74_02745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792187.1"
                     /translation="MPSRTPEPSPSPSPVSASSTEFQDAAETSSEIARATSENAVREA
                     GRLEGALANLAKARSDENLALSREALSTARAAYFQMESAVLFDGDEFGREAASLPEPV
                     ALPGNGEDNRFSQIESLLKSYEHPASEGLVVSRFSLERNQTVADLKIAAENLRLAMEK
                     FSASWGKDDPANFRNAVFLSDPEAAIGQVLQGLMKTSDWIAAQAGALPAGEIAPRLRA
                     IQSYCLGMYDDGAGRVTGRGLTALVASRSPGEAEDLASHLAGSLRDLEEGPPDPNRVR
                     SQFLEISGRMQRAAEALGFRVESSVDDSVVTDSTSGRTPN"
     gene            complement(1333..2565)
                     /locus_tag="VIS74_02750"
     CDS             complement(1333..2565)
                     /locus_tag="VIS74_02750"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEY5792188.1"
                     /translation="MATYTFRALAEHGGQETGRIESLDKAAAFRILRERKLRPLSLER
                     LGAENPVDAPVRPGGRSWSRLSNAAVHSFTEELAQLLEAGLQLERALRVIEGRKEKTA
                     IPGIAGFVRQQIREGKRFSSALRNTGGAFSDLYCNMVAAGEAAGALPSILRRQSQYLA
                     LISELRRRVVMALVYPSVVFSAGVVLLLIFMTFLLPQLTMLLSKTGQTLPLVTRILIG
                     TSEFFGHYWWALLAAAALIVAGLRMWRKTPKGRKIWDRAILEAPLLGPILRIQFLAEF
                     LQTLSTLVSNGITLLHGLTLMQQATRNVSLQALIGALADKVGEGAAFSRVLRQNAFFP
                     PVLTDIIAVGEETGDLGGALERAAVRYDQEFTMRIQQLTTLIQPLTILVVALFVGLVA
                     YSMITGILSSVSGLRLNQ"
     gene            complement(2565..>3193)
                     /locus_tag="VIS74_02755"
     CDS             complement(2565..>3193)
                     /locus_tag="VIS74_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HEY5792189.1"
                     /translation="KLDGVVQIAVKPEINLTFATGLRSILRGDPNVIMVGEMRDFETA
                     EIAIRAALTGHLVFSTLHTNDSVGGITRLIDMGVEPFLVGSSVRAFIAQRLVRRLCPL
                     CKIPESIPSDRLEELGFSGGTSLFKAHGCEACRNSGYQGRIAIYEICQVTPALQDMIQ
                     RKVPGGELKKLAAAEGMHSLRENGWERVKEGITSIGEVMRVTAAEARN"
BASE COUNT          578 a          991 c         1050 g          574 t
ORIGIN      
        1 cgggcgttcg aggtttgcgg cctgcggcat tggcgcgttt tgcgagatgg tgtcgcggcg
       61 gaatcttctt ttgcgctgac ggttccagtt ggagctgcca tcacgttgtc gcagtcgaag
      121 tggtgaagca acttccacaa cgacgaaacg gtgggcattc cgttgaccgc ggcgggatct
      181 tcgattgcga ggacggtcac gaaattcgtg agcgattcac agtcatgtcc agatccgagt
      241 cagcgtgtcc ggccttaacg cgcgccgtgg gagctatccc gtcagcgcgc cacgggcggc
      301 ggatcgcaga aatctctcgc tacctgcaaa tcctggccct gttagccttc ctttgttcct
      361 gccggtacga gaccgacccc gttcctgcac cgacgccgat tcctttcccg acgcccgtgc
      421 cgtcacgcac tcccgaaccc tcgccttcgc caagcccggt gagcgcctcc agcacggaat
      481 ttcaggacgc cgcggagacg agttcggaaa ttgcccgggc aacgagcgag aatgcggtcc
      541 gcgaggctgg ccgcttggag ggggccttgg cgaatcttgc gaaagcacgg agcgatgaaa
      601 atctggccct ttcccgcgag gctctttcca ccgcccgggc ggcctatttc cagatggaga
      661 gtgccgttct cttcgacggc gacgagttcg gccgcgaggc cgcttcgctt cccgagccgg
      721 tcgcgcttcc ggggaacggg gaggacaacc gcttttcgca gatcgagagc ctcctcaagt
      781 cctacgagca tcccgcctcg gagggcctgg tggtttcccg cttttcgctg gagcgcaacc
      841 agacggttgc cgacctcaaa atagccgccg aaaacctgcg gctggccatg gagaaatttt
      901 ccgcctcgtg gggaaaagac gacccggcca actttcggaa cgccgtgttt ctctcagacc
      961 cggaggcggc gatcggccaa gtcttgcaag gcctgatgaa aacctcggac tggattgcgg
     1021 cgcaagccgg cgcgcttcct gccggcgaga tcgcgccgcg cctgcgcgcg atccaaagct
     1081 attgcctggg aatgtacgat gacggtgccg gcagagtgac gggccgcggc ctcacggcgc
     1141 tcgtcgcctc gcgctcgccc ggggaggccg aagacctcgc cagccatctg gccgggtccc
     1201 tgcgcgatct ggaggaaggg ccgcccgatc cgaaccgcgt ccgctcccaa tttctcgaaa
     1261 tctccggccg catgcagcgg gccgccgaag cgctgggctt ccgtgtggag agttccgtgg
     1321 acgactccgt tgtcactgat tcaacctcag gccggacacc gaactgagaa ttccggtgat
     1381 catggaatag gccaccagcc cgacaaaaag cgccaccacc aggatggtga gaggttggat
     1441 cagggtggtc agttgctgga tgcgcatggt gaattcctgg tcgtagcgca cggccgcccg
     1501 ttccagcgct ccccccaggt cgccggtctc ctcgccgacg gcgatgatgt cggtgagaac
     1561 gggcggaaag aacgcgtttt gccgcaggac gcgtgaaaac gccgcacctt cacccacctt
     1621 gtccgccagc gcgccgatca gggcctgcag ggacacattg cgagtcgcct gctgcatgag
     1681 cgtgagcccg tgcaggagcg tgatgccgtt ggagaccagg gtggaaaggg tctggaggaa
     1741 ttccgcgagg aactggatgc ggagaatcgg gccgagcagc ggcgcttcca gaatggcgcg
     1801 gtcccagatt ttccggccct tcggagtctt tcgccacatc ctcaggccgg ccacgatcag
     1861 ggcggcggcg gcgagaagcg cccaccagta gtggccgaaa aactcgctgg ttccgatcag
     1921 gatgcgggtc acgagcggaa gggtctggcc ggtcttgctg aggagcatgg tcagttgcgg
     1981 cagcaggaac gtcatgaaaa tcagcagaag caccacgccc gccgagaaga ccacggaggg
     2041 ataaacgagg gccatcacga cccggcggcg caattcggag atgagcgcga ggtattggct
     2101 ctggcggcgc aggatgcttg gcagggcgcc cgccgcttct cccgcggcga ccatgttgca
     2161 atacaggtcc gaaaaagccc cgccggtgtt cctcagggcc gacgagaacc gcttgccttc
     2221 gcggatttgc tggcggacga aacccgcgat cccgggaatg gcggtctttt ccttgcgccc
     2281 ctcgatcacg cgcaatgcgc gctccaactg gaggcccgcc tcgaggagct gggcgagttc
     2341 ctcggtgaag gaatgcacgg ccgcgtttga aagcctggac catgatctcc cgcctggccg
     2401 gacgggagcg tccaccggat tctccgcgcc gaggcgctcg agagaaagcg gccgcagttt
     2461 tcgctcccgc aggatgcgga acgcggcggc cttgtcgagg gattcgatcc ggccggtttc
     2521 ctgcccgccg tgttccgcca gagcgcgaaa ggtgtaggtc gccatcagtt ccgggcctcg
     2581 gcggcggtca cacgcatcac ctcgccgatg gacgtgatgc cttccttcac gcgttcccag
     2641 ccgttctcgc ggagggaatg catgccttcg gcggcggcga gttttttcaa ttctccaccg
     2701 ggcaccttgc gctggatcat atcctgcagc gcgggcgtga cctggcagat ttcgtagatc
     2761 gcgatgcggc cctggtagcc gctgttgcgg caggcctcgc agccatgggc tttgaaaagg
     2821 gaggtcccgc cggaaaaccc gagttcttcg aggcgatcgg aggggatgga ttcgggaatt
     2881 ttgcaaagcg gacagagacg cctcaccagc cgctgggcga tgaaggcccg gacggaggag
     2941 cccacgagaa acggctccac tcccatgtcg atcaggcgcg tgatgccgcc cacagagtca
     3001 ttcgtatgca aagtactgaa aacgagatgg ccggtgagcg cggcgcggat ggcgatttcc
     3061 gccgtttcga agtcgcgcat ttcgccgacc atgatgacat tgggatcgcc gcggagaatg
     3121 ctgcgcaatc cggtggcaaa ggtgaggttg atctcgggct tgacggcgat ctgcacgacg
     3181 ccgtcgagtt tgt
//
LOCUS       DATDAY010000145         4329 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_1568035, whole genome shotgun sequence.
ACCESSION   DATDAY010000145 DATDAY010000000
VERSION     DATDAY010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 4329)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4329)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4329
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1568035"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            504..863
                     /locus_tag="VIS74_02760"
     CDS             504..863
                     /locus_tag="VIS74_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
                     /protein_id="HEY5792190.1"
                     /translation="MAQLGIRAEDLVETFARSSGPGGQNVNKVSTAVTLLHKPSGLRV
                     VASDSRSQITNRKLALERLLDEIERRKELQRQSRLADISKARRQRAKRSRGTKRKLVE
                     SKRRRGETKKMRGKVSD"
     gene            1341..1724
                     /locus_tag="VIS74_02765"
     CDS             1341..1724
                     /locus_tag="VIS74_02765"
                     /inference="COORDINATES: protein motif:HMM:NF022495.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2750 domain-containing protein"
                     /protein_id="HEY5792191.1"
                     /translation="MSDDSASAEENYEAFIARVRETGKVWGLCRDSEEWAYSESNEYE
                     DAEVLLFWSDRERALAHQQDEWKQHRPTAIELEDFLERWLPGMDDDGALVGPDWDANF
                     EGLEVTPEELAERLIDEGEEGDEES"
     gene            1769..2686
                     /locus_tag="VIS74_02770"
     CDS             1769..2686
                     /locus_tag="VIS74_02770"
                     /inference="COORDINATES: protein motif:HMM:NF013431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792192.1"
                     /translation="MAATHHTFEKDGIIYDQLTAGDLSIAVARRGAELISLRQGEMGF
                     LYRDGLTDKPAAGWANHATVMGYFLHRLWKEESVYRGQAIRSGNHGFIRHFVFDPPAF
                     DAAAGSLTYEVQPERIPPEAYPLKVRLRLAYRLAGGRLTVEFHFINEEAELDAHVSFG
                     LHPGFAVASVQTARVEFPAGTYLRHLAPGNFLDGQIEPVAHAGGEMPFDKALLPGSFL
                     LELSQVPERTFVLSDPETGRRVTLDFAEVPYLTVWSEGADYICIEPCWGLPDSNPPAP
                     FEQKAGIEKISAGAALIRAFAIAPEQIKG"
     gene            2691..2852
                     /locus_tag="VIS74_02775"
     CDS             2691..2852
                     /locus_tag="VIS74_02775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792193.1"
                     /translation="MKLIGFFLKSLLFVGLTFAFLVLFQHGPAHFANHCLAEFDYWQS
                     KIANTASGN"
     gene            2962..4293
                     /locus_tag="VIS74_02780"
     CDS             2962..4293
                     /locus_tag="VIS74_02780"
                     /inference="COORDINATES: protein motif:HMM:NF024579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792194.1"
                     /translation="MQLKNFGYTIQLLQTVLKEVPEFLGARQLARKAAVAKTSNKKSL
                     MSGLSSASFSTMKVQSLVKKNPVAALEAVEKLLESDPYSPAANQLLREAALAAKLPEI
                     AALALETIIEGNPRDTKTMHELAKHYMAHEEPARAVDIYARILEITPSDLLAVKGGKD
                     ASARASMQRGGWETAETYRDLIKDKEIAVSLEQQGRVVRSEEMIDQQLAELHEKIQQE
                     PQSVDTARKIATLYEDKEDFINATEWYDYTIQLTAGSDSALIRKAADLRVKHYDLTLQ
                     QWEDYLATLEPDSTEAGNAAAEIERYKAQRAELILAEAKLRVERNPTDLVLRFELGEI
                     LVANGNYQEAIPELQKARNNPNVRLRAMNLLGKCYQGKHMLDLSAKMFTEAASEISAM
                     DSTKKEITYNLGLVYEEMGDTQKSVDCMKQIYEVDYGYRDVAARVEGSYAG"
BASE COUNT          951 a         1220 c         1376 g          782 t
ORIGIN      
        1 gcgtccggta ttcctgcatc agcgcgatct cccgctccgt ccgggcgacg gcgggattga
       61 tggtgctgca ctcggctcgt atgtgctcca caatctggtg ctgttcatcg agcggcggga
      121 gggccaactg gaagttctta atctgctcaa tattcacatg gggagcggtc gatcccatca
      181 catacaacga cgcttggaga tagacaccgt aagattgttc cgcaccttgt cggcagcgag
      241 ccagagcctg ccccttgagc tgagggagga agcgacggag tttgatcggg cagagtggcg
      301 ggcaggaggc acaagcctga aaccgtgtca aattcaaaga gttttgacga ggaaaacccc
      361 attcaggtcg cctcgatcaa aatctggaga ttttcacacg taagtgccga cagcttgagg
      421 cagtcatcgc gagtccgcat gcaattacgg tccttgcgaa accgcaaaga tcccctgaca
      481 ctgaacgcat gtttgcggaa cgcatggctc aacttggcat tcgcgcggag gatctcgtcg
      541 agacctttgc gcgttcgtcg gggccaggcg gccaaaatgt aaacaaggtt tcgacagccg
      601 ttaccctgct gcacaaaccc agcggtttgc gggtggtggc gagtgattcc cggtcgcaga
      661 tcaccaaccg gaaactggcc ctggagcggc tgctcgatga aattgagcgg aggaaggaac
      721 ttcagcgcca gtcccggctg gccgatattt ccaaggcccg gcgccagcgc gccaaacgct
      781 cccgcggcac gaagcggaag ctggtggaat ccaaacgccg ccgaggcgaa acaaagaaaa
      841 tgcgcggaaa agtctcggat tagagcgatt ctcaaaagag attcccgaaa agggattcaa
      901 tgcaggggca gccggagcga agacagcctg tcccgcggaa gcgggacagc ccgaggggcg
      961 cacgaagcgc gacaattgtg ccaaccgccc cgcgtaaacg gggatgcggg gtccccgttt
     1021 ccccgcaaac aaaggcggtt ggcacaagcg cctcaatgaa tcgctttttc ggaaatctct
     1081 tttgagaaat actataacag agccggaact ccccttggaa aatctgcgcc gccaaacttg
     1141 gttggcattt ccgcagatgg ctagctttgg atacgtctgg catgcatata ggcgacccgc
     1201 agcgccgcag ccgttcgtaa agcgcgtccc aattcgcatc catgcggtgg atttgcccga
     1261 aaaccgcggc gaatgcagcc atgctttggt cgcgaacctg gcttgagcaa ttcgggcgat
     1321 tccggttagg ctggcgcact atgagcgacg attcggccag cgcggaggaa aattacgaag
     1381 ccttcatcgc ccgcgtgcgg gagacgggca aggtgtgggg attgtgccgg gactcggagg
     1441 agtgggcgta ttccgaatcc aacgaatacg aggatgcgga ggtgctgctt ttctggtcgg
     1501 accgggaacg cgcgctcgcg catcagcagg acgaatggaa acagcaccgg ccgacggcca
     1561 ttgagctgga ggattttttg gagcgctggc tgccgggcat ggatgatgac ggggcgctgg
     1621 tggggccgga ctgggacgcg aatttcgaag gcctcgaagt cactccggag gaactggcgg
     1681 agcggttgat cgacgagggc gaagaaggtg acgaggaatc ctgaattctt cgcttggatc
     1741 ggcgcggcgg gcggcgaata ttgcccccat ggctgccacg caccacactt ttgaaaagga
     1801 cggaatcatt tatgaccagc tgaccgcggg ggatttatcc atcgcggtgg cgcggcgggg
     1861 ggcggagttg atcagcctgc gccaggggga gatgggtttt ctttatcgcg acgggctcac
     1921 ggacaagccg gcggcgggat gggcgaatca cgcgacggtc atgggttatt tcctgcaccg
     1981 cttgtggaag gaggagtcgg tgtatcgcgg ccaggcgatt cggagcggca accatggatt
     2041 catccgccat ttcgtttttg atccgccggc gtttgacgcg gcggcgggct cgctgaccta
     2101 cgaggtgcag ccggagcgga ttccgccgga agcgtatccg ctgaaggtgc gcctgcgcct
     2161 ggcgtatcgc ctggcgggcg ggcggctcac ggtggagttc catttcatca atgaggaggc
     2221 ggaactcgac gcgcacgtga gcttcgggct gcacccgggc ttcgcggtgg cgtcggtgca
     2281 aacggcgcgg gtggagtttc ccgcgggcac gtatctgcgc catctggctc ccgggaattt
     2341 tttggacggc caaatcgagc cggtcgcgca cgcgggcggg gagatgcctt tcgacaaggc
     2401 gctgctgccg gggtcgttcc tgctggagct cagccaggtg ccggagcgga cgtttgtcct
     2461 gagcgatccg gaaacgggcc ggcgcgtgac cctggatttc gcggaggtgc cttatctgac
     2521 ggtctggagc gagggggcgg actacatttg catcgagccg tgctgggggc tgccggacag
     2581 caatccgccg gcgccgtttg aacaaaaagc gggcatcgag aaaatttccg cgggtgcggc
     2641 gttgattcgg gctttcgcaa tcgccccgga gcagataaaa ggatgaccgc atgaaattga
     2701 tcggattttt tttgaagagc cttcttttcg tgggattgac gttcgcgttc ctggtgctgt
     2761 tccagcacgg gcccgcccac tttgcgaacc actgcctggc ggaatttgat tattggcagt
     2821 ccaagatcgc aaacaccgcg tctgggaatt gacactccgg cagcccggag ggatattgtc
     2881 cacttccccg ctatggccgt caaaaccgag aaagacctcc ccgaacacct gcgggccatg
     2941 tggctaaaag ccatgagtgc catgcagctc aaaaatttcg gctacaccat ccagctcctt
     3001 cagacggtgc tgaaggaagt gccggaattc ctcggcgccc gccagctcgc ccgcaaggcc
     3061 gccgtggcca aaacctccaa taagaaaagc ctgatgagcg ggctctccag tgcttcgttt
     3121 tcgacgatga aggttcagag cctggtgaaa aagaatccgg tcgcggcgct cgaggcggtg
     3181 gaaaaactcc tggagtccga cccctacagc ccggccgcaa accagttgct gcgcgaagcg
     3241 gccctggcgg ccaagctgcc ggaaatcgcc gcgctggcgc ttgaaaccat catcgaaggc
     3301 aatccgcggg acacgaaaac catgcacgag ctggccaaac attacatggc ccacgaggaa
     3361 ccggcccgcg cggtggatat ttatgcccgg attctcgaga tcaccccgtc cgacctgctt
     3421 gcggtcaagg gcggcaagga cgcgtcggcg cgcgcctcca tgcagcgggg cggatgggaa
     3481 accgccgaaa cctaccggga tctcatcaag gacaaggaga ttgcggtttc cctggagcag
     3541 caaggccgcg tggtgcgcag cgaggagatg attgatcagc agctcgcgga gttgcacgag
     3601 aaaatccagc aagagccgca aagcgtggac accgcgcgca agatcgccac cctctacgaa
     3661 gacaaagagg atttcatcaa cgccacggag tggtatgatt acacgatcca gctcaccgcc
     3721 ggatcggact ccgcgctcat ccgcaaggcc gccgacctgc gggtcaaaca ctacgatctc
     3781 accctccaac aatgggagga ttatctcgcc acgctcgaac cggacagcac ggaagccggg
     3841 aacgcggccg ccgagatcga gagatacaag gcccagcggg cggaactcat tctggcggaa
     3901 gccaaacttc gagtggaacg aaaccccacc gacttggtgc ttcgcttcga attaggggaa
     3961 atcctggtgg caaatggaaa ctaccaggaa gccattcccg agctgcaaaa ggcccggaat
     4021 aatcccaatg tccgcctccg cgcgatgaac ctgctcggga aatgctatca gggcaaacac
     4081 atgctcgatc tttccgccaa gatgttcacc gaagccgcgt cggaaatctc cgccatggat
     4141 tccaccaaaa aggagatcac ctacaacctc ggcctcgttt acgaagagat gggcgacacg
     4201 cagaaatccg tggactgcat gaagcagatt tacgaggtgg actacggcta ccgcgacgtc
     4261 gccgcccggg tggaaggttc ctacgccgga tagggcggat tcgattaaag tttgcgcaaa
     4321 atggagggc
//
LOCUS       DATDAY010000146         2808 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_1650593, whole genome shotgun sequence.
ACCESSION   DATDAY010000146 DATDAY010000000
VERSION     DATDAY010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2808)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2808)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2808
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1650593"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(480..2072)
                     /locus_tag="VIS74_02785"
     CDS             complement(480..2072)
                     /locus_tag="VIS74_02785"
                     /inference="COORDINATES: protein motif:HMM:NF024821.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY5792195.1"
                     /translation="MEIRPLKLSPASQRKAEATALFARGLFDQESEGPEVALESYRKV
                     LALDPGYTGLAVHVAQEDLRRGDVARALATLKDSLRANPRSPVASIALAKIYLLNLRK
                     PETAELYAQKALAAARSIDSYECLWDVYQFTGQRRKADQLLVKASQDDSRDPEFWLEL
                     TRLFQRQTALVSGPEEEWSARANAAADKAAELGFNQPLILNKAAEYFFLSGQLAKAAP
                     LYERILKLDPDFPDVLEKLAGAYMGIPDYEHALPVLRKLIGENPQSLNAYDQLAQLYR
                     SRQEDAQALEAMQKALLLAPYVYERHVAVIGLMLDLQRYETALMYANGAAEKFPQSGL
                     LTYLRAISLCRLERAAEALPLFERALVEGSTQQGGDFLDAMFYFEYGIAAEQAKAYAR
                     AAELFKKSIELDPGNAPRTCNYLGYMWVERGENLAEAEMLIRRALKTDPENGAYIDSL
                     GWLYFKQGKYADALIELLRAAERLSQPDTVVYEHIGDAYRQLGRNPEALLYWQKAAQL
                     DPENKALAKKIDESAEKVASKP"
     gene            complement(2359..>2808)
                     /locus_tag="VIS74_02790"
     CDS             complement(2359..>2808)
                     /locus_tag="VIS74_02790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HEY5792196.1"
                     /translation="FSWGATEVKVPDLVDAIIDITETGSSLRANKLRIVETLLVSYPV
                     LAANQDAWVDEWKRAKISQLALLLKAALNARDLVGLKLNLADEKLKNLLEALPALRNP
                     TVSPLAQAGWVAVETVIEERVVREIIPQLKALGAEGIIEYPLNKVVY"
BASE COUNT          521 a          907 c          804 g          576 t
ORIGIN      
        1 cgaagcgctc acggcaagcc tcaaggagca agccttgcag attcaaagag tgagcctcca
       61 gctcgccgcg ggccaacccg cccccgcccc gcgcctggcg ctcagcactc cctaaatccc
      121 acgcccagcc ccaaaaccga cgcaggcgct ctcctttcgg aaaccgccac ccgagttttc
      181 gcggcgaacc tccaggctcc agcgaaccgc tgagtcaatg gcccaactcc gcctccttcg
      241 ctccaaccct ctttggcagg ctctctggaa cggacccttt gacctcaccc acttttaatc
      301 actcccgatg gcgttagcgc aatgggggaa aatttgtgcc aagccgccgt tccgggcctc
      361 ctgaaataaa tccgccgatt acgcagattc cgccgatttt tgggaatctt taggaaagct
      421 ggcggatgaa ttccgatctt tgagactttt tcgctgccgc ttggcctcgg gccagcgcct
      481 caaggcttgc tcgccacttt ttcggcggat tcgtcgattt tcttcgccag cgccttgttt
      541 tccgggtcga gctgcgccgc cttctgccag taaagcagcg cctcgggatt ccggcccagc
      601 tggcggtagg cgtccccgat gtgctcatac acgacggtgt ccggttgcga cagccgctcg
      661 gcggcccgca ggagctcgat cagcgcatcg gcgtatttcc cctgcttgaa atagagccag
      721 ccgaggctgt cgatgtaagc gccgttttcg ggatcggtct tgagcgcgcg ccggatcagc
      781 atctccgcct cggccaggtt ttcgccgcgt tcgacccaca tgtagcccag gtaattgcac
      841 gtccgggggg cattgccggg gtcgagttcg atggactttt tgaacagctc cgccgcccgg
      901 gcatacgcct tcgcctgctc ggccgcgatg ccgtattcaa aataaaacat ggcgtcgagg
      961 aaatcccccc cctgctgggt gcttccctcc acgagcgccc gctcaaacaa gggcagggct
     1021 tccgccgccc gttcgagccg gcagaggctg atggcgcgga ggtaggtcag caggccggat
     1081 tggggaaatt tttccgccgc cccattcgca tacatcagcg cggtctcata acgctgcaaa
     1141 tccagcatga gtccgatcac cgccacatgg cgctcataca cgtagggcgc cagcagcagc
     1201 gccttctgca tggcctccag cgcctgggcg tcttcctgcc gggaacggta gagctgcgcc
     1261 agctggtcat aggcattgag cgactgggga ttttccccga tcagcttgcg caacaccggc
     1321 agggcatgct cgtagtcggg aatccccatg taggcgccgg ccagcttttc gagcacatcc
     1381 gggaaatccg gatccagctt gagaatgcgc tcatagagcg gcgccgcctt ggcaagctgg
     1441 ccgctgagga aaaaatactc cgccgccttg ttcaggatga gcggctggtt gaagcccagc
     1501 tccgccgcct tgtccgccgc cgcgtttgcc cgggcgctcc attcctcctc agggccggaa
     1561 accagggcgg tttgccgctg aaacagccgg gtgagttcga gccaaaactc gggatcgcgc
     1621 gaatcgtcct gcgaagcctt caccagaagc tggtcggctt tccggcgctg tccggtgaat
     1681 tgatagacgt cccacaggca ttcgtaggag tcaatcgaac gggcggccgc cagcgccttc
     1741 tgggcataaa gctccgccgt ctccggtttg cggagattca gcaggtagat cttagccagc
     1801 gcgatcgagg cgaccggact ccgcggattc gcccttagcg agtccttgag cgtggcaagc
     1861 gcccgcgcga catccccgcg gcgcaggtct tcctgggcga cgtggacggc gaggccggta
     1921 tagccgggat ccagcgccag caccttgcgg tagctttcca gcgccacctc gggcccttcg
     1981 ctttcctggt cgaagagtcc gcgggcaaac aacgcggtgg cctccgcctt tcgctggctg
     2041 gccggactca gtttgagcgg ccggatttcc accgcgccgt cgtccaggaa ggcgtccgtt
     2101 tccgaattcg cccacgcccc gctagcggag acaagagcca gcagaaggaa accactcctg
     2161 cgaatgatcc cccgcctcca acaattccga gccgctccag cgcgcatgct ggaactttag
     2221 caattaagat ttgtagcgca aacgcttctt gtgccgattt tccttcatcc gcttacggcg
     2281 cttgtgcttt gcgatctttg cttttcttct ttttttcagg gaacccatgt ggaggcgaga
     2341 cttctaaact tctaatgact aataaacaac cttgttaaga ggatattcga taattccttc
     2401 ggcaccaagg gctttaagtt gaggaataat ctcgcgaaca acgcgttctt cgatgacagt
     2461 ttccactgca acccagccgg cctgtgccag aggcgaaacg gtaggattcc tcaaggcggg
     2521 aagcgcctca agcaaattct tcaatttttc atctgccagg ttcaatttga gcccgacgag
     2581 gtcccgtgca ttgagcgcgg ctttgagcag cagggccagc tgggaaatct tcgcgcgctt
     2641 ccattcgtcc acccacgcgt cctggttggc ggcgaggacc gggtaggaaa ccagcagggt
     2701 ttcgacgatg cgcagcttgt tggcgcgcag cgaggaaccc gtctcggtga tgtcgatgat
     2761 ggcgtccacc agatccggca ccttcacctc ggtggcgccc caggagaa
//
LOCUS       DATDAY010000147         2141 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_1262995, whole genome shotgun sequence.
ACCESSION   DATDAY010000147 DATDAY010000000
VERSION     DATDAY010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2141)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2141)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2141
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1262995"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..197
                     /locus_tag="VIS74_02795"
     CDS             <1..197
                     /locus_tag="VIS74_02795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792197.1"
                     /translation="AHRIEAFDEVAGWRTLAAGTTIGHQRLHRWEPLRTRHLRIILVR
                     FLAPPALASVGIYGGRESAE"
     gene            283..358
                     /locus_tag="VIS74_02800"
     tRNA            283..358
                     /locus_tag="VIS74_02800"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:316..318,aa:Ala,seq:ggc)
     gene            724..1056
                     /locus_tag="VIS74_02805"
     CDS             724..1056
                     /locus_tag="VIS74_02805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792198.1"
                     /translation="METRVNYTDEPKDGLELPASGFKVLTRAEEKAWGLEPPAPGTEY
                     ERTEKDGVVRLAPKRGGARPGAGRKPTGHVRMHILVSKEARQKILRLAKKQKITISEA
                     VDRMAKSA"
     gene            1136..>2141
                     /locus_tag="VIS74_02810"
     CDS             1136..>2141
                     /locus_tag="VIS74_02810"
                     /inference="COORDINATES: protein motif:HMM:NF018305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEY5792199.1"
                     /translation="MARRMTGNSPIERDDFRETFGSGHPGSRHDPLEYSPREWRGLEK
                     LPLFWRLQLGGWLGWTVWSLPINFAIYADIGTAFLISCVREITGFALTLGLREIYRRT
                     PVGRLGVWAMAPLIVVICLLAAGIDVASLIYLQGLLGLGAEDYNQTDMLGYLFCLRTL
                     LFVSWSGLYLSFRGLLATKAQADLLFQAEKLRHEAELQMLRAQINPHFLFNAFNTILS
                     EGKGNPGVVALVKALSEYLRYSLSCRHEGFVSLGAEIDAITSYLEVEKARFGGDLILE
                     IAVTDTARAAGVPGVIIQPLVENAIKYGRETCTPPLKVRVAAETGPHALRIEVSNTGR
                     W"
BASE COUNT          403 a          669 c          657 g          412 t
ORIGIN      
        1 cggcgcaccg catcgaggcc ttcgacgaag ttgccggatg gcggacgctg gcggccggca
       61 ccaccattgg ccaccagcgg ctgcaccggt gggaaccgct ccgaacccgc cacctccgga
      121 tcattctggt gcgtttcctc gccccgccgg cgctcgcctc ggtcggcatc tatggcggtc
      181 gcgaatccgc cgaatagaaa atccatagat tccgcttgcc tcgtttcgag gcagaaacta
      241 ccttcactca cccgctcggg aaaccgaacg gattttttat ctggggtctt agctcagttg
      301 gtagagcacc acaatggcat tgtgggggtc aggggttcga atcccctagg ctccaccaga
      361 ttttcgggtg gagttgtgtg aggcaaaaga ttcgaacctc gggttcggcc gcatccgcgc
      421 gaagcgcgaa acctttttac ccgcgcagct ggcactccgc aggtcgcgcc gtgacaatcc
      481 cccaggctcc gccagatttc cggaggccga tgacctttaa aggtattggt tctgggttcg
      541 agtcccagcc aacccacttt gaatgtgacc agccatgtcc ggtgtttcgc ctgcggggga
      601 aaaaagtgcg ctggcagatg gtggggttcg atgggcgggg aatattgacc gtgcgcttta
      661 cccttcgcgc cggcaaaatg cgggtcattg gcgcagggta ttggagggcc caaaggaaag
      721 tgtatggaaa ccagagttaa ctacacagac gaacccaagg atgggttgga gcttccggcc
      781 agtggcttca aggtgctcac tcgtgccgag gaaaaggctt ggggccttga accgcccgcg
      841 ccgggaactg agtatgagcg caccgaaaaa gacggcgtgg tgagactcgc gccgaagcgc
      901 ggcggcgccc gtcccggcgc cggccgaaag ccgactggcc atgtgcgcat gcacattctg
      961 gtctccaagg aggcccgcca gaaaatcctc cgcctcgcga aaaaacaaaa aattacgatt
     1021 tcggaagcgg tggacagaat ggcgaagtcg gcttaagcaa tcaaaaggtt gtccgcaagg
     1081 aagcgcaagc cggcgcgaat ccggcattgc acccgggcaa tttcgtccta ctctcatggc
     1141 ccgtcgtatg acgggcaatt caccaatcga gcgggacgat tttcgcgaga cgtttggctc
     1201 gggccatccc ggatcgcgcc atgacccgct ggagtattct ccgcgcgaat ggcgggggct
     1261 cgaaaaactg ccgctcttct ggcggctgca actcggcggc tggctcggct ggaccgtctg
     1321 gtccctgccc atcaatttcg ccatctacgc ggacatcggc accgctttcc tcatcagctg
     1381 cgttcgcgag atcaccggct tcgccctcac cctcggcctg cgtgagattt accgccgcac
     1441 gccggttggc cggctggggg tttgggcgat ggcgccattg atcgtcgtca tctgcctgct
     1501 cgcggcgggg atcgacgtcg ccagcctcat ttaccttcag ggcctgctcg ggctcggggc
     1561 ggaggactac aaccaaaccg acatgctggg ttatctcttt tgcctgcgca ccctgctgtt
     1621 tgtttcctgg agcgggctct acctctcctt ccgcggcctg ctggccacga aggcgcaggc
     1681 cgatctcctg tttcaggcgg aaaaactgcg ccacgaggcg gaactccaga tgcttcgcgc
     1741 ccaaataaat ccccactttt tgttcaacgc cttcaatacc atcctcagcg agggaaaggg
     1801 caaccccggc gtcgtcgcgc tcgtcaaggc gctgtccgaa tacctgcggt attcgctgtc
     1861 ctgccggcac gaggggtttg tctcgctcgg cgcggagatt gacgccatca ccagctacct
     1921 cgaagtcgag aaggcccgct tcggcggcga tctcattctg gaaattgccg tcacggatac
     1981 cgcccgggcc gcgggcgtgc ccggggtgat cattcagccg ctggtggaga acgccatcaa
     2041 atacggccgg gaaacctgca ccccgccgct caaggtgcgg gtggccgcgg aaaccgggcc
     2101 gcacgcgctg cgcatcgagg tgagcaacac cggccgctgg c
//
LOCUS       DATDAY010000148         4340 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_1530126, whole genome shotgun sequence.
ACCESSION   DATDAY010000148 DATDAY010000000
VERSION     DATDAY010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 4340)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4340)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4340
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1530126"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..653
                     /locus_tag="VIS74_02815"
     CDS             <1..653
                     /locus_tag="VIS74_02815"
                     /inference="COORDINATES: protein motif:HMM:NF020952.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEY5792200.1"
                     /translation="MPFIVVIIDELADLMQTAPADVEAAIARITQMARAAGIHLIVAT
                     QTPRAQVVTGVIKANIPSRIAFQVASSLDSRVILDDNGAEKLLGQGDMLYLPPGTSRM
                     TRSQGVLVTDEEIRRVVDFVSTQGKPAFEASIHEKLTNFSAGDEDVTDEDEELVDKCL
                     EIIRQERRASTSMLQRRLRLGYTRAARVVDILEQRGIVGPRDGARDREILVDLDSL"
     gene            659..1366
                     /locus_tag="VIS74_02820"
     CDS             659..1366
                     /locus_tag="VIS74_02820"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY5792201.1"
                     /translation="MGRGSEIQFASLGKRLALARETRGLSIEQVAKSTRIRPHLLRHL
                     EGDDFTHFSHPSYLRLYFMDYARFLNVPLQEIRDWLPDAGCPGSENYEYITGLGAEGA
                     GQIRKEYHYPRSKGGDFLRRTVRGVLVLAMLGLVTALALLIRDLVRIGPSGVSAGSAI
                     AADAAIPAEPADLSTAGDSEFVRAESAEAKLGAGEMTGLSDPSAPSASPASVLEQPAN
                     LSLISPAVSAPAEAQIR"
     gene            1373..2758
                     /gene="rimO"
                     /locus_tag="VIS74_02825"
     CDS             1373..2758
                     /gene="rimO"
                     /locus_tag="VIS74_02825"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HEY5792202.1"
                     /translation="MPAPISIAPPRKVGMISLGCAKNLVDAEIMLGDAAMRGFEITSD
                     PNEADVLVVNTCGFIDSAKQESIEAILEAHQQRGLQKREGQKLVVSGCLSQRFSKELS
                     HELPEVDAFIGLDQVGKTGEILEKLFAAPAGPEPLNLVTRGSRYIPDWNTPRFRLTPA
                     HSAYVKIAEGCNHPCSFCVIPQMRGRHRSRTIDSVTAEVRNLVENGVREINLISQDTT
                     YFGMDFWEQKAGPRQPVDSGRGPTLTRLLDSLAEVEGDFWIRLLYTHPAHWSPELIRA
                     IAQNPKVAKYVDMPLQHIHEEMLGTMRRETSRQHIEDLIDSLRAGIPGLGIRTTFIVG
                     FPGETDAHFESLLEFIETRRFERLGVFSYSQEDGSRAAKMEAQIPAKVKNARHRKAMK
                     LQQKIAAEIARGQVGRELRVLVETPQTARTEHDAPEVDCRVILEEPAAVGEFRTVRVT
                     GTQVYDLIARA"
     gene            2825..3097
                     /locus_tag="VIS74_02830"
     CDS             2825..3097
                     /locus_tag="VIS74_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WXG100 family type VII secretion target"
                     /protein_id="HEY5792203.1"
                     /translation="MAQAIMDPGEVRRFAQELKRFNEDVQGRAASLQARFAALGSSWQ
                     DQEHQQFSEEFITTMKVLRKFMEISEKHTPYLLRKAQRIEDYLDQR"
     gene            3100..3624
                     /locus_tag="VIS74_02835"
     CDS             3100..3624
                     /locus_tag="VIS74_02835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792204.1"
                     /translation="MATQARIDSIETLELFRSRLIVFTNKMQLQNGEIGNEVRRTRAW
                     LQTEQRMKWEGEIRRRRQILDQAEQELLSARMSSLHGDLSGRQNAVRKAARALAEAED
                     KLRKVREWSRNFDAATDPLVRKLDGLGRLIDSEMPKAIAFLSQTQRILAGYLETAVPA
                     EHPAGPPPEEPGQP"
     gene            3621..3833
                     /locus_tag="VIS74_02840"
     CDS             3621..3833
                     /locus_tag="VIS74_02840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792205.1"
                     /translation="MSGRVNAAPLVQAGRDLLLEWEQVRERWSDANRREFEKQFLAEL
                     PLEIARVAEVVAEIDVILRKVRNDCE"
     gene            3826..>4340
                     /locus_tag="VIS74_02845"
     CDS             3826..>4340
                     /locus_tag="VIS74_02845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792206.1"
                     /translation="MNSSIHPRAQRSFELRDNLKELAAEFSQRREQLARDIARRRREE
                     NQGFQAGMDRIEAELAAETARLEAEFANAERRGRERFEARRRRIERIRDSGNANLLDQ
                     GRRARERWLGNLQMKHFYATRNLPVALQAEETAAAEFLAALSREEAALADLRARARQA
                     FGDPAAFFRAES"
BASE COUNT          839 a         1331 c         1408 g          762 t
ORIGIN      
        1 aaatgccgtt catcgtcgtg atcatcgacg aactggccga cttgatgcag accgctccgg
       61 cggacgtgga ggcggcgatt gcccgcatca cccagatggc gcgcgcggcg gggattcatt
      121 tgatcgtcgc cacccagacc ccgcgggcgc aggtggttac cggggtgatc aaggcgaaca
      181 ttccgagccg catcgccttc caggtcgcct cgagcctgga cagccgcgtg attctcgatg
      241 acaacggcgc ggagaagctc ctgggccagg gcgacatgct gtatctcccg ccgggcacct
      301 cccgaatgac gcgctcccag ggcgtgctcg tgaccgatga ggaaatccgc cgcgtggtgg
      361 actttgtttc cactcagggc aagccggctt ttgaggccag cattcacgaa aaattgacca
      421 atttttccgc cggagacgag gacgtgaccg acgaggatga ggagttggtg gacaaatgcc
      481 tggagatcat caggcaggag cgccgggcct cgacttcgat gctgcagcgc cgcctccgcc
      541 tgggttatac gcgggcggcg agagtggttg acattttgga gcaacgcggc attgtcggtc
      601 cgcgagacgg ggccagggac cgcgaaattc tggtggattt agattcgtta tgagcgcaat
      661 ggggagggga agtgagattc aatttgccag tctcgggaaa agactggcgt tggcccggga
      721 gacaaggggt ttgtccatcg agcaagtggc caaaagcact cgcatccggc cgcatttgct
      781 gcgtcacctg gaaggggatg atttcaccca tttttcgcat ccttcctacc tgcggcttta
      841 tttcatggat tacgcccggt ttctgaacgt gccgctgcag gaaatccggg attggctgcc
      901 ggatgccgga tgtccgggca gcgagaatta tgaatacatc accggtttgg gcgcggaggg
      961 tgccgggcag atccggaagg aataccatta tccccgcagc aaaggcggcg atttcttgcg
     1021 ccggaccgtc cggggcgtgc tcgttcttgc catgctcggc ctggtcaccg ccctggcgct
     1081 tctcatccgg gacttggtga gaattggccc ctccggcgtc tccgccggat ccgcgatcgc
     1141 ggcggatgcg gccatccccg ccgaaccggc ggacctttcc acggccggcg attccgaatt
     1201 cgtcagggcc gaatcggcgg aagcgaaact gggcgccgga gaaatgaccg ggctttcgga
     1261 cccgtccgcc ccctccgcca gcccggcctc ggtcctggag caaccggcga atctttccct
     1321 gatctcaccc gccgtttcag ccccggcgga ggcgcagatt cgataaccgc cgatgccggc
     1381 cccaatttcc atcgccccgc cccgcaaggt gggcatgatc agcctcggct gcgcgaaaaa
     1441 cctcgtggat gccgagatca tgcttggcga tgccgccatg cgcggattcg agatcacttc
     1501 ggatccaaac gaggcggatg tgctggtggt gaacacgtgc ggcttcattg attccgccaa
     1561 gcaggagtcc atcgaagcca ttctcgaggc ccaccagcag cgcggcctgc agaaacggga
     1621 agggcagaag ctcgtcgtca gcggatgcct ctcgcagcgt ttttcgaagg aactcagcca
     1681 tgagctgccg gaagtggatg ccttcatcgg cctcgatcag gtcggcaaga ccggggaaat
     1741 cctggaaaaa ttattcgcgg cgccggccgg cccggagccg ctgaatctgg tgacgcgcgg
     1801 ctcgcgctac attccggatt ggaatacgcc gcgttttcgc ctgacgccgg cgcattcggc
     1861 ctacgtaaaa atcgcggagg ggtgcaatca cccctgcagc ttttgcgtga ttccgcagat
     1921 gcgcgggcgg catcgcagcc ggacgattga ttccgtgacc gcggaggtgc ggaatctggt
     1981 cgaaaacggc gttcgggaaa tcaatctcat cagccaggat acgacctatt tcggcatgga
     2041 tttctgggag cagaaagccg ggccgcgcca gccggtggac tccggacgcg ggccgacgct
     2101 gacgcgcctg ctcgattccc tcgccgaggt cgagggggat ttctggattc gcctgcttta
     2161 cacacatccc gcgcactgga gcccggagct gatccgggcg atcgcccaaa atcccaaggt
     2221 cgccaaatac gtggatatgc cgcttcagca cattcacgag gagatgcttg gcacgatgcg
     2281 ccgcgaaacc agccgccagc acatcgagga tctcattgat tcgctgcgcg cggggattcc
     2341 agggttgggg atccgcacga cgttcatcgt cgggtttccc ggcgaaacgg acgcgcattt
     2401 cgaatcactg ctggagttca tcgaaacccg ccggtttgag cgcctcgggg tattttccta
     2461 ctcccaggaa gacggctcgc gcgcggcgaa aatggaggcg cagattcccg cgaaggtcaa
     2521 aaatgcgcgg catcgcaagg cgatgaagct gcagcaaaaa atcgccgcgg aaatcgcccg
     2581 cgggcaagtc ggccgggaac tgcgcgtgct ggtcgaaacc ccgcagaccg cccgcaccga
     2641 gcacgacgcg ccggaagtgg attgccgggt gattctggag gaaccggcgg ccgtcggcga
     2701 attccggacg gtccgagtca ccggcacgca ggtctatgac ctgattgcgc gggcataggt
     2761 cctgcgcgcg aaaattcgcg ggcgtggttg ataattgccg cgttttgcgc cagattgagg
     2821 cgcgatggct caggcaatta tggatcccgg cgaggtgcgg cgtttcgcgc aggaattgaa
     2881 acgctttaat gaggacgtgc agggccgggc ggcctcgctc caggcgagat tcgccgctct
     2941 ggggagctcc tggcaggatc aggaacatca gcaattttcc gaggaattta tcaccacgat
     3001 gaaggttctg agaaagttta tggagatctc cgaaaaacac acgccctacc tcctgcgcaa
     3061 ggcgcagcgc atcgaagact acctggacca acggtaacca tggcgactca ggcccgcatt
     3121 gattccatcg aaacgctgga gttgttccgc tcccgattga tcgtcttcac gaacaagatg
     3181 cagctgcaga atggcgagat cggcaacgaa gtgcgccgga cccgcgcctg gctgcagacc
     3241 gagcagcgga tgaaatggga gggggaaatc cgccgccgcc gccagatcct cgatcaggcc
     3301 gagcaggagc ttctcagcgc ccgcatgtcg tccctgcacg gggatctttc cggccgccag
     3361 aatgccgttc gcaaggccgc ccgcgccctg gccgaagccg aggacaagct gcgcaaggtc
     3421 cgggaatgga gccgcaactt tgacgccgcg acggatccgc tggttcgcaa gctcgacggg
     3481 cttggccggt tgatcgattc cgaaatgccc aaagccatcg ccttcctttc ccaaacccag
     3541 cggattctgg ccggctatct ggaaaccgcc gttccggccg agcatcccgc cggcccgccc
     3601 ccggaggagc ccgggcagcc atgagcggcc gggtaaacgc ggcgccgctg gtccaggcgg
     3661 gccgggatct tctcctggaa tgggagcagg tccgggagcg ctggagcgac gccaaccgcc
     3721 gggaatttga gaagcaattc ctggccgaac ttcccctcga gatagcccgg gtggcggagg
     3781 tggtggcgga aatcgacgtg atcctgcgaa aagtccgaaa tgattgtgaa tagttccatc
     3841 cacccgcgcg cccaacgcag tttcgaactc cgggataacc tgaaggaact cgccgccgag
     3901 ttttcgcaaa ggcgggagca actggcccgg gacatcgccc gccgccgccg cgaggagaat
     3961 caaggctttc aggccggcat ggatcggatc gaagcggaat tggcggcgga aaccgcccgc
     4021 ctcgaggcgg agtttgccaa tgccgaacgg cgggggcgcg agcgttttga agcccgccgc
     4081 cgccggattg agcgcatccg cgactccggc aacgccaatc tgctcgacca gggccgccgg
     4141 gcccgcgaac gctggctggg caacctgcag atgaagcatt tttacgccac gcgcaacctg
     4201 ccggtcgcgc tgcaggcgga ggaaacggcg gcggcggagt ttttggccgc gctttcccgc
     4261 gaggaagccg ccctggcgga tctgcgcgcc cgggcccgcc aggcgttcgg cgaccccgcc
     4321 gcttttttcc gggcggaatc
//
LOCUS       DATDAY010000149         2922 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_1130872, whole genome shotgun sequence.
ACCESSION   DATDAY010000149 DATDAY010000000
VERSION     DATDAY010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2922)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2922)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2922
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1130872"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..587
                     /locus_tag="VIS74_02850"
     CDS             <1..587
                     /locus_tag="VIS74_02850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792207.1"
                     /translation="LTRAEALRWLGAFLPLSVIGSAASNLYSTAFLKRFPFPTDFGHE
                     GDAAWGVIIAPFVKLAVTPRVCADFVVHGRGHDLPAAGQAARFEQMAALARETFRPVC
                     TDPELREVFHHLSAAWEANHRVWRWLAALDGIADTAEEQRKYIHLLEADAARLKREVE
                     LLGGMPVSRWTPFLQSKHMAGLLRPLKRWLRLSK"
     gene            584..1519
                     /locus_tag="VIS74_02855"
     CDS             584..1519
                     /locus_tag="VIS74_02855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792208.1"
                     /translation="MSAGPGFLNRESPPQARVVIARSAGMLGNRLFLSAYFLAHASVH
                     GYRLFNPALGEYAALFESTWADPWCRYPARAGGSEKTGGSARSRCRLVGAAAAILSRL
                     PGGVAGVRALDIARSHDAADVPYDLNGPEFMSALRQSRCLLVQGWKFRDDAHLKTQHG
                     VIRDYFTPRSAIRARVREIIERARAGVDVLIGVHVRQGDYRAWQGGRFYFDTPLYAAW
                     MRAAAALFPGARVGFLICSNAAQDPAAFAGLRVSFGSGVVVEDLYALAECDRLMGPPS
                     TFSLWASYYGRVPLHMLTRADQAIHPVSFTFHDAA"
     gene            1527..2447
                     /locus_tag="VIS74_02860"
     CDS             1527..2447
                     /locus_tag="VIS74_02860"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY5792209.1"
                     /translation="MITPTLNAAATVREAVESVLAQGMAGVEHLVMDGASTDGTLEVL
                     AEFPHLQVHSARDGGLYQAMNAGIRRASGRWLVFLQADDVLLPGALAAVAAAIQRNPG
                     VEIVNGGAEARRGFRPDSELVWRRSEPRECELTAANVVLGEPMVNARVFRRDLVGQLG
                     GFSTDYRLASDRDFLLRAVELNCPQVTIPHLVYRYCWHAGSRTMTEGNALSRLLTEEN
                     LRIAEAHLARGGEEARPPLRQWHRQQSVQAAMMALEALDARGWLAAARRGLKVDRAWG
                     AAFLREFGSSLRGFIGRGFRTRSQVWRAGR"
     gene            complement(2457..>2922)
                     /locus_tag="VIS74_02865"
     CDS             complement(2457..>2922)
                     /locus_tag="VIS74_02865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792210.1"
                     /translation="PAQLWALAHFPQGAKVENSYAPSWKRLGKLKITEERLPAATGRA
                     ELFKKMLGKDSAAIQEGITRHETGDLGDTFTLEALRRRNPDFVAYSTQVFEFSGSDEA
                     QRFYGALDAGVAGYGKVFEAASRPAAAWSYPSELDFIPARMVILERNPPPGE"
BASE COUNT          411 a          935 c         1019 g          557 t
ORIGIN      
        1 ccctgacgcg ggcggaagcc ctccggtggc tcggcgcctt cctgccgttg agcgtgatcg
       61 gcagcgccgc cagcaatctg tattccacgg ctttcctgaa gcggttcccg tttcccaccg
      121 attttggcca cgagggggat gccgcctggg gggtgatcat agcccccttc gtcaaactcg
      181 cggtgacgcc gcgggtctgc gcggattttg tggtgcacgg gcgggggcat gaccttcccg
      241 ccgccgggca ggcggcgcgg tttgagcaaa tggcggccct ggcccgcgaa accttccggc
      301 ccgtctgcac ggatccggag ctgcgggagg tgttccatca tctctccgcc gcgtgggagg
      361 ccaaccatcg cgtctggcgc tggctggcgg cgcttgatgg catcgcggac accgcggagg
      421 aacagcggaa atacattcat ctcctcgaag ccgatgccgc ccggctcaag cgggaggtgg
      481 aactgctggg cggcatgccc gtttcccgct ggacgccgtt cctccaatcg aaacacatgg
      541 ccggcctcct gcgcccgctg aagcgctggc tgcgcctctc caaatgagtg ccggccccgg
      601 tttcctgaac agagaatccc ctccgcaagc ccgcgtggtg attgcccgca gcgccggcat
      661 gctgggcaac cggctgtttt tgagcgccta ttttctcgcg cacgccagcg ttcatggtta
      721 ccggctgttt aatcccgcgc tgggcgaata cgccgctctg tttgaatcca cctgggcgga
      781 tccgtggtgc cgctaccccg cgcgggcggg cggatcggag aagacggggg ggagcgctcg
      841 aagccggtgc cggttggtgg gagcggcggc ggcgattctc tcgcggttgc ccggcggagt
      901 ggccggcgtg cgggcgctgg acatcgcccg ctcccacgat gcggcggacg tgccttacga
      961 tctgaacggc ccggaattca tgagcgcgct ccggcagtcc cgctgccttc tggtgcaggg
     1021 ttggaaattc cgggatgacg cccatctgaa aacgcagcac ggggtgatca gggattattt
     1081 taccccccgt tccgccatcc gcgcgcgcgt ccgcgaaatc atcgaacgag cccgggcggg
     1141 agtggatgtc ctgatcggcg tccacgtccg gcagggggat taccgggcct ggcagggcgg
     1201 ccggttttat tttgacaccc cgctttacgc ggcctggatg cgcgcggcgg cggcgctatt
     1261 tcccggcgcg cgcgtgggtt tcctgatttg ttcgaatgcg gcccaggatc cggcggcctt
     1321 tgcgggtttg cgggtttcct ttgggtccgg cgtggtggtt gaggaccttt acgcgctggc
     1381 ggagtgcgat cgtttgatgg gaccgccgag caccttttcc ctctgggctt cctactacgg
     1441 gcgcgtgccg ctgcacatgc tgacccgcgc ggatcaggcc attcaccccg tgagttttac
     1501 cttccatgac gccgcctgaa ttcacggtga tcactcccac cctcaacgcc gcggcgaccg
     1561 tgcgggaggc cgtcgaaagt gtgctcgccc aggggatggc cggggtggag catctcgtca
     1621 tggacggcgc ctccacggac ggaacgctgg aagtcctcgc ggaatttccc catctccagg
     1681 tccattccgc gcgggacggc gggctttatc aggcgatgaa cgcggggatt cgccgggcgt
     1741 cgggccgctg gctggtcttt ttgcaggccg atgatgtgct gctgcctggc gcgctggcgg
     1801 cggtggcggc ggcgattcaa cgcaaccccg gcgtggagat cgtcaatggc ggcgcggagg
     1861 cgaggcgggg tttccgtccg gacagtgagt tggtctggcg gcgaagcgaa ccgcgggagt
     1921 gcgagctgac cgcggccaat gtcgtgctgg gggaacccat ggtgaatgcg cgggttttcc
     1981 ggcgcgatct cgtcggccag ctgggcggtt tttccacgga ctaccgcctg gcctcggatc
     2041 gggatttcct gctgcgggca gtcgagctca attgtccgca ggtgactatt ccgcacctgg
     2101 tttaccgcta ttgctggcat gccggatcgc ggaccatgac ggagggcaat gcgctcagcc
     2161 ggctcctgac ggaggaaaat ctccggattg ccgaggcgca tctcgcccga ggcggggagg
     2221 aagcccgccc gccattgcgg caatggcatc gccagcaatc cgtgcaggcg gccatgatgg
     2281 cgctcgaggc gctggacgcg cgggggtggc tggccgccgc ccggcgcggt ctgaaagtgg
     2341 accgcgcctg gggcgccgcc tttctgcggg agttcggctc gtcgctccgc ggtttcatcg
     2401 ggcggggatt ccgcacccgc agccaggtct ggcgcgcggg ccgctgattc ccgcccttat
     2461 tccccgggcg ggggattccg ttcaaggatg accattcgcg cgggaatgaa atccagctcg
     2521 gaggggtagg accacgccgc ggccggccgg gaggccgcct cgaaaacctt gccatacccg
     2581 gccaccccgg cgtcgagcgc cccgtaaaaa cgctgggcct cgtcgctgcc gctgaactcg
     2641 aaaacctggg tggaataggc cacgaaatcg ggattccggc gccgcagggc ttccagtgtg
     2701 aacgtatctc cgagatcccc ggtttcatgc cgggtgattc cctcctggat cgccgcggag
     2761 tccttgccga gcattttctt gaacaattcc gcccggccgg tcgccgcggg cagccgttct
     2821 tcggtgattt tgagtttgcc cagccgtttc cacgagggtg cgtagctgtt ttccaccttg
     2881 gctccctggg ggaaatgcgc gagcgcccag agctgggccg gc
//
LOCUS       DATDAY010000150         2036 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_1957618, whole genome shotgun sequence.
ACCESSION   DATDAY010000150 DATDAY010000000
VERSION     DATDAY010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2036
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1957618"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..509
                     /locus_tag="VIS74_02870"
     CDS             <1..509
                     /locus_tag="VIS74_02870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792211.1"
                     /translation="AKTSVAAATPPPAPAAPGEASVLPAYTVSTFKSVFHRPEFWIAN
                     GAAGVAFLALLGYWSFLRISGGKAAQVAQARRQSRKLLDEMAARGTETRDFCEKGAAF
                     LESRLGAASPEALAGAAFSPEEAARLVPMLTLRDEARYGVGRGNGQLSAETRAEILDI
                     LKKWEAKT"
     gene            506..1258
                     /locus_tag="VIS74_02875"
     CDS             506..1258
                     /locus_tag="VIS74_02875"
                     /inference="COORDINATES: protein motif:HMM:NF019844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HEY5792212.1"
                     /translation="MNGLSRWIAGLMGLGIAFLPLRAADEFYEANEQYGAANYEQAAR
                     TYRLALDKGELSAEIYYNLALAESKAGKSAEASLNFQRALTLNPALEPARRELLALAS
                     RTGMEIPPATWVDRLAALAPAASWWTGGAVAAWAGAFILLGGLFARRRRGTLILLGAV
                     VFLAGKGTLFLAWSSDPLIQNRPLAVITAEGTDHLRSAPVENSATVTRLPGGSTVALL
                     AERGKWAYCAALDGKKGWIERDNLQPVMPRES"
     gene            1255..>2036
                     /locus_tag="VIS74_02880"
     CDS             1255..>2036
                     /locus_tag="VIS74_02880"
                     /inference="COORDINATES: protein motif:HMM:NF022750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HEY5792213.1"
                     /translation="MIRALLLLVVVGTAGAADWNSQLTSPEAGPFPEVRPFDAKFTFG
                     WSGIPAAAGEASLRHEGEEYQVEARGGTEGLAAQLWKIRVENRTRARRQQFISEEVRQ
                     IEQYASYRISITGKFQTDGVWRERLREPSSNPGGWKFLRVSPLRDMASAMLFVRSQPL
                     RNGDEIRLLAFPGDSPYLVRVQIEGRESIRVMNETRPAIRLRLFLQKIETSGPNKGKL
                     QEHAKFRSGVVWVSDDENRFPLRAEVNLFIGYVYVEVTALEF"
BASE COUNT          344 a          643 c          691 g          358 t
ORIGIN      
        1 cggcgaaaac ttcggtggcc gccgccactc ccccgccggc tcccgcggcg ccgggcgaag
       61 cctccgtgct gccggcctac accgtttcca ctttcaagtc cgtgttccac cgccccgaat
      121 tttggatcgc caatggggcg gcgggcgtgg cgtttctggc cctgctgggt tactggagtt
      181 ttctgcggat ttccggcggg aaagccgcgc aggtggccca ggcccggcgc cagtcccgga
      241 agctgttgga cgagatggcg gcccgcggaa cggaaacccg ggatttctgc gaaaagggcg
      301 ccgcgttttt ggaaagccgc ctgggcgcgg cttcgccgga ggccctggcc ggcgcggctt
      361 tttccccgga ggaagccgcc cggctggttc cgatgctcac gctgcgcgac gaagcccgct
      421 acggcgtagg ccgcggcaac ggccagctct ccgccgaaac gcgcgcggaa attctggaca
      481 tcctgaaaaa atgggaggca aaaacatgaa cgggctgagt cgctggattg cgggattgat
      541 gggtctcggg atcgccttcc tgccgctgcg ggccgcggac gaattttacg aggccaatga
      601 gcaatacggc gcggccaatt acgaacaggc cgcgcggacg tatcgcctcg cactggataa
      661 aggcgagctc tccgcggaga tttattacaa tctcgcgctt gcggaatcca aggctggaaa
      721 atccgccgag gccagcctga attttcagcg cgcgctgacg ctgaaccccg cgctcgaacc
      781 cgcgcggcgg gagttgctgg ccctcgccag ccggacgggc atggaaatcc ccccggccac
      841 gtgggtggac cgcctggcgg cgctggcgcc ggccgcctcc tggtggaccg gcggcgcggt
      901 ggcggcctgg gcgggcgcgt ttattttgct cggagggctc ttcgcccggc ggcggcgggg
      961 gactttgatt ttgctcggag ccgtggtctt tctcgccggc aagggcacgc tctttctcgc
     1021 gtggtcgagc gatccattga ttcaaaatcg cccgctggcg gtcatcaccg ccgagggaac
     1081 ggaccatctg cgcagcgcgc cggtggaaaa ttccgccacc gtcacccgcc tgccgggcgg
     1141 ctcgaccgtg gcgctcctgg cggagcgcgg aaaatgggct tactgcgcgg cgctggatgg
     1201 caaaaagggc tggatcgaac gggataacct tcaacccgtg atgccgcgcg aatcgtgatc
     1261 cgcgcgctgc tgctgctcgt cgtcgttgga accgccgggg cggcggattg gaattcgcag
     1321 ctgacttctc cggaggccgg gccgttcccg gaggtgcggc catttgacgc gaagttcacg
     1381 tttggctggt cgggcattcc cgcggcggcc ggggaggctt ccctgcgtca cgagggggag
     1441 gaataccaag tcgaagcccg cggcggcacg gaaggactcg ccgcgcagct ttggaaaata
     1501 cgagtggaaa accggacgcg cgcgcggcgg cagcaattca tttccgagga ggtccgccag
     1561 atcgagcaat acgcgtccta ccggatttcc atcaccggaa aattccagac cgacggcgtc
     1621 tggcgcgagc gtttgcggga gccgtcgtcg aatccgggcg gctggaaatt tctgcgcgtc
     1681 agcccgctgc gcgacatggc gagcgccatg ctgttcgtgc gcagccagcc cctgcggaac
     1741 ggcgacgaaa tccgcctgct ggccttcccc ggcgattcgc cgtatctcgt ccgcgttcag
     1801 atcgaaggcc gcgaaagcat tcgggtgatg aatgaaaccc gcccggcgat tcgcctgcgt
     1861 ttgttcctgc aaaaaatcga aacctccgga ccgaacaagg gcaaattgca ggaacacgcc
     1921 aaattccgca gcggcgtcgt gtgggtttcg gacgatgaaa accgcttccc cctgcgcgcg
     1981 gaggtgaatc tcttcatcgg ctacgtttat gtggaggtca ccgcgctgga gttcca
//
LOCUS       DATDAY010000151         6581 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_2013886, whole genome shotgun sequence.
ACCESSION   DATDAY010000151 DATDAY010000000
VERSION     DATDAY010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 6581)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6581)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6581
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2013886"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..906)
                     /locus_tag="VIS74_02885"
     CDS             complement(<1..906)
                     /locus_tag="VIS74_02885"
                     /inference="COORDINATES: protein motif:HMM:NF021364.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding domain-containing protein"
                     /protein_id="HEY5792214.1"
                     /translation="MKPAKPAVSSNPPPPPPDLLGLQRLMAAAVMRPLDARDRTRKRW
                     SDGRPTAEIAEGFIKPNDRLTSIERLEIYNRQYWFRLLDCLYDDYPGLRAVLGSGRFM
                     RLLRAYLTAHPSESFTLRNLGAGMVAFLRRNPKWTEPHSELAVDMARLEWAQIEAFDN
                     PARPPLQLGQLSNRPPGEIFLRLQPHLTLLDLAFPIDEWVVKLLREDDRMRSEASNAV
                     EAPDPAVRRSIARKIPRSPIWLAVHRVENEVYFKRLEAAQFQMLRALESGLSLEAACA
                     EVPGLTPDQLQAWFANWAALGWFWMK"
     gene            complement(854..1699)
                     /locus_tag="VIS74_02890"
     CDS             complement(854..1699)
                     /locus_tag="VIS74_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 domain-containing protein"
                     /protein_id="HEY5792215.1"
                     /translation="MPANRFNGSTDYGIGIGLRVPHYRHILEKKPVVDWFEIISENFM
                     VDAGRPLEVLDQILEQYRVVQHGVALYFGSAQPLNRGHLKKLKRLVKRTNTPWLSDHL
                     CWGSVDGRYTHDLLPMPYTFEAARVTARRIREARDFLEVPICVENVSSYAEYHVSEMT
                     EWEFLTEVVERADCGILLDVNNIYVSSQNHGFDPYDYLNHIPHHRVGQIHIAGHSKFE
                     KYILDTHDHPVLDPVWKMYDHAIRQTGRTATLLEWDDRIPSFDEVHHEARKASRFLEP
                     APAAA"
     gene            complement(1755..2015)
                     /locus_tag="VIS74_02895"
     CDS             complement(1755..2015)
                     /locus_tag="VIS74_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792216.1"
                     /translation="MNTKHLLAGAAVAGLLTGASASAFAKTTNDNQSGTKVTTLADKG
                     THSCKGQNACKGQGGCKSGDNSCKGKNSCKGKGGCATDGSHK"
     gene            complement(2101..2598)
                     /locus_tag="VIS74_02900"
     CDS             complement(2101..2598)
                     /locus_tag="VIS74_02900"
                     /inference="COORDINATES: protein motif:HMM:NF019301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEY5792217.1"
                     /translation="MNATFSFFARLIGWYKTFIICAESAQSALLLLIRLVWGLQFAQA
                     GWGKCQDIPRVAGFFTEIGIPFPELNAHVVAGTELFGGIFLALGLVSRLVTLPLIVSM
                     AVAYLTTEREALQALWGNPDPFLTAAPFLFLFASILILVFGPGKISLDYWIGKKLNYF
                     SSRAE"
     gene            complement(2676..3425)
                     /locus_tag="VIS74_02905"
     CDS             complement(2676..3425)
                     /locus_tag="VIS74_02905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792218.1"
                     /translation="MDNEKARLILSAYRPGGSDAGDPVFAEALEQARRDPELGAWLAQ
                     QRAFDSRMKAGLQSIPIPRNLKASLLVSGKVSDFPASRSFSKTFPWLAVAAGLVALVW
                     LTFFLLSPPSPRVSFARVDAQLRSLADAHQHAFETPMHDLQKIRAWLVDHGAPHDFTL
                     PDALAALPGLGCEVISVEGIKVSILCFPRENGRVAHLYVINRAQLLDPPSNPEPAFQQ
                     KGAYAQAAWSDGKQTYLLVERGDYEAIKGLL"
     gene            complement(3418..3972)
                     /locus_tag="VIS74_02910"
     CDS             complement(3418..3972)
                     /locus_tag="VIS74_02910"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEY5792219.1"
                     /translation="MSTRDFEILVEAHYEDLYRFAYSLARNPDEACDLTQQTFAVYAE
                     KADQIREPLKRKSWLFTTLYREFLKTRSRGCRVVSMEEADLEIHQAVVESGVQRETEQ
                     QEMLEELAALDADQRAILTLFYLDQHSYKDIAGILGIPAGTVMSRLSRAKEALRARFE
                     KGRKTDGIKIVPIRSDRRKEEGHG"
     gene            4098..4937
                     /locus_tag="VIS74_02915"
     CDS             4098..4937
                     /locus_tag="VIS74_02915"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY5792220.1"
                     /translation="MSEAAIVVTPPPQIKKPWRLPGGPKLQNWLFGWWTIGVFIFLYV
                     PIVLLVIFSFNESRLNIRWEGFWLKWYGVLFQNERILGAFKNSLIVAAATTVLSTFIG
                     TVGAWMLHRYRYPFKQFLDLLVLVPMVMPEVLMGVSLLLFFVSMKIGLGYLTLIIAHT
                     TFCFPFVLVGVQARLQGIDPFLEEAAMDLGATPIQAFWKVIVPYLMPAIISGALMSFT
                     LSFDEYIVTVFTMSAQAQTFPVTVYGMVKRGLDPSLNALSTLFILATVILVLFTETLK
                     KLK"
     gene            4939..6081
                     /locus_tag="VIS74_02920"
     CDS             4939..6081
                     /locus_tag="VIS74_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HEY5792221.1"
                     /translation="MKRSALAPTIGALAAVVLLAGCDKPATTTQAPTPEPAPAEMAAP
                     DATPAVAATPAAPTNTEEKVLNLYCWSEYVPQSVIDEFQKATGIKVAVENYASNEEML
                     TKLLAGGGTYDLIQPSEYVIEALIKEKLLEPLDHAAIPNLKNIAPEFQNMAFDPGNKY
                     SVPWMAGTVGIVINTGLVKDDIKTYDDVFQAKNKGNIVVLDDAREIVSWALASQGIPI
                     NDVTDENLEKIKPILSKWLPLVKVYDSDSPKTSMLNGDVALGVVWSGEGALLLNEDKK
                     FKWVLPVGAHLFVDSLAIPKGAKHPKNAEAFINFILLPEVSKLISADFPYLNPNAPAR
                     ALLTPEEQANTASFPTAEELQKMEIFTDIGPQATKVDEIVTTVKSQ"
     gene            6091..>6581
                     /locus_tag="VIS74_02925"
     CDS             6091..>6581
                     /locus_tag="VIS74_02925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792222.1"
                     /translation="MATTVIPQGGGSDHQRRPGLGAWLFLAPMLVWLILFVVVPSVLL
                     VIMSFAERGTLGRVVYNFSFDNYTRVFSSWPILKIFLLSIWYALLTTVICVIVGYPVG
                     YFIGRLPERWRNLCLTLVMIPFWTSFLIRTYAWVSLLSKEGLINGLLLSAHIIPSPLG
                     MMYT"
BASE COUNT         1336 a         2004 c         1915 g         1326 t
ORIGIN      
        1 cttcatccag aaccacccca gcgcggccca gttggcgaac caggcctgca actgatccgg
       61 cgtgaggccg ggaacttccg cgcaggcggc ttccaggctc agcccggact cgagcgcccg
      121 cagcatttgg aactgggcgg cttccaggcg cttgaaatac acctcgttct ccacgcggtg
      181 caccgccagc cagatcggcg aacgcgggat tttccgcgcg atgctccggc ggaccgccgg
      241 atccggcgcc tccaccgcgt tgctggcttc cgagcgcatc cggtcatcct cgcgcaggag
      301 tttcaccacc cattcgtcaa tgggaaacgc gaggtccagc aaagtcagat gcggctggag
      361 ccgcagaaaa atttcgccgg gcgggcggtt ggacagctgc ccgagctgca gcggcggccg
      421 ggccgggttg tcgaaggcct cgatctgcgc ccattccagc cgggccatgt ccaccgccag
      481 ttccgaatgg ggctcggtcc atttcggatt ccgccgcaaa aaggcgacca tgccggcgcc
      541 gagattccgc agggtgaagg attcggaggg atgcgccgtg agataagcgc gcagcagccg
      601 cataaaccgg ccgctgccca gcacggcgcg caggccgggg tagtcatcgt agaggcagtc
      661 cagcagccgg aaccaatatt gccggttgta gatttccagg cgctcgatgg aggtcagccg
      721 gtcattcggc ttgatgaagc cctccgcgat ttcggcggtg ggccggccgt cgctccagcg
      781 cttccgggtg cggtcccgcg catccagcgg ccgcatgacc gcggcggcca tcaaccgctg
      841 caatccgagc aggtcaggcg gcggcggggg cgggttcgag gaaacggctg gctttgcggg
      901 cttcatggtg gacttcatca aaggaaggaa tgcggtcgtc ccactccagc agcgtcgcgg
      961 tgcggccggt ctggcggatg gcatgatcat acattttcca gaccgggtcg agcaccgggt
     1021 ggtcgtgggt gtcgaggatg tatttctcga atttggaatg cccggcgatg tgaatctggc
     1081 ccacgcgatg atgcgggatg tgattcaagt agtcgtaggg gtcaaatcca tgattttggg
     1141 aggagacgta aatattgttc acgtcgagca ggatgccgca gtccgcccgc tccacgacct
     1201 cggtgaggaa ctcccattcg gtcatttcgg acacgtggta ttccgcgtag ctgctgacat
     1261 tctccacgca gatcgggacc tccagaaaat cccgcgcctc gcgaatccgc cgcgcggtga
     1321 cgcgggcggc ctcgaacgtg tagggcatgg gcagcaggtc gtgggtgtaa cgcccgtcca
     1381 cgctgcccca gcagaggtga tcggaaagcc acggcgtgtt cgtgcgtttg accagccgct
     1441 tgagtttttt gagatggccg cggttcagcg gctgcgcgga gccgaaataa agcgccacgc
     1501 cgtgctggac gacgcggtat tgctcgagga tttgatccag cacttcgagc ggccggccgg
     1561 catccaccat gaaattttcg gaaatgatct cgaaccagtc cacgaccggc ttcttttcca
     1621 ggatgtgccg gtaatggggc acgcgcaggc cgatgccgat gccgtaatcc gtggaaccat
     1681 taaaacgatt cgcaggcata tcgttaaagc gaactggagg gaagaagcgg ccctcccccc
     1741 agtccttcga tcccttactt gtgcgaaccg tcggttgcgc agccgccctt gcctttgcaa
     1801 gagtttttgc ctttgcaact gttgtctccg gacttgcagc cgccctggcc cttgcaggca
     1861 ttttgtccct tgcaggagtg ggtgcccttg tcggccaagg tcgtgacctt agtgccggat
     1921 tggttgtcgt tcgtggtttt cgcaaaggcc gaagccgatg cgccggtgag cagtccggca
     1981 acagccgcgc ccgcgaggag atgtttggtg ttcatggttt gtgtctttca gtaggtataa
     2041 cgctgaaaac ccgcccggcg gattcatcat ttcgtaaaat aagagaaacg agcgaatgat
     2101 ttattccgcc cggcttgaaa aataattgag ttttttgccg atccaataat cgagggagat
     2161 ttttcccggc ccgaatacca ggattagaat ggaggcgaat aaaaataaaa agggcgcggc
     2221 cgtgaggaac ggatcgggat tgccccacaa ggcctggagc gcctcccgtt cggtggtgag
     2281 ataggccacg gccatcgaga caatgagcgg caatgtgaca agcctggaaa ccaatccgag
     2341 cgccaggaag attcccccga aaagctccgt gcccgcgacg acgtgggcgt tcaactccgg
     2401 aaacggaatt ccgatctccg taaagaagcc cgccaccctg gggatatcct ggcatttccc
     2461 ccagcccgct tgggcgaatt gcagccccca gaccagccgg atgagaagca gcagggcgga
     2521 ttgagccgat tcggcgcaga tgatgaaggt tttataccaa ccgatgagcc gggcgaaaaa
     2581 tgagaaggtt gcgttcatat cccatttgag cgatgggagc cggttttatt ccggaaattt
     2641 cccggcccag cctacctcag cccgccgcca gcaaatcaga gcaatccctt gattgcctcg
     2701 taatcgccgc gttcgacgag cagataagtc tgcttgccgt ccgaccaggc cgcctgggcg
     2761 taagccccct tttgctgaaa cgcgggctca ggattggagg gcggatcgag cagttgagcc
     2821 cgattgatca cgtagagatg cgcgacccgg ccattctccc gcggaaagca gagaatggaa
     2881 accttgatcc cctcgactga aatcacctcg caaccgagtc cgggaagcgc agccagggca
     2941 tcgggaagcg taaaatcatg cggcgcaccg tgatccacca gccaggcgcg aattttttgc
     3001 agatcgtgca tcggcgtttc gaaggcgtgc tgatgtgcgt cggcaagcga gcggagctgc
     3061 gcgtccacgc gggcgaaact cacgcgcgga ctcgggggcg acagcagaaa aaacgtcaac
     3121 caaaccagcg cgacaagtcc cgccgcaacc gccagccagg gaaaggtttt tgaaaacgaa
     3181 cgcgaggctg gaaaatccga caccttgccg gaaaccagca gcgaggcctt cagatttcgg
     3241 ggaatcggaa tggattggag ccccgccttc attcgcgaat caaaggcgcg ttgttgcgcc
     3301 aaccaggctc cgagttcggg atcacgccgg gcctgttcaa gcgcttccgc aaacaccgga
     3361 tccccggcgt cgctcccgcc ggggcgatac gcgcttaaga tcagcctggc tttttcgtta
     3421 tccatgtcct tcctcctttc tccgatccga gcgaatcggg acgatcttta ttccgtccgt
     3481 ttttcggccc ttctcaaatc tggcccgcag cgcctctttg gcccgggata atcgcgacat
     3541 caccgtgccg gcgggaatcc cgagaatccc ggcgatgtcc ttgtaggagt gctggtccag
     3601 atagaaaagc gtgagaatcg cgcgctgatc cgcatccaga gccgccaatt cctcgagcat
     3661 ctcctgctgc tccgtttccc gctgcacgcc ggattccacg acggcctggt gaatttccaa
     3721 atccgcttcc tccatgctga ccacgcggca tccgcgggat cgggttttca gaaactcccg
     3781 ataaagcgtg gtaaagagcc agctctttcg ctttaggggt tcccggatct gatcggcttt
     3841 ttcggcgtaa accgcgaagg tctgttgcgt cagatcgcag gcctcgtccg gattccgggc
     3901 caggctgtag gcaaaacgat agagatcctc gtagtgcgcc tcgaccagaa tttcaaaatc
     3961 gcgcgtggac atgcctcttt tttcattgga gcaggaagcg cgggttctat tccatgaaaa
     4021 ataaaattat cccatccacc tggctccggc tcattttcat gctggacgcg tgagggccag
     4081 gtgtttaaat cggactcatg tctgaggcgg ccattgtagt cactcccccg ccccaaatca
     4141 aaaagccctg gagattgccc ggcggcccca agctgcaaaa ctggctcttc ggatggtgga
     4201 ccatcggcgt ctttattttt ctgtatgtgc cgatcgtcct gctggtcatt ttttcgttta
     4261 acgaatccag gctgaacata cgctgggagg gtttttggct gaaatggtat ggcgtgcttt
     4321 ttcaaaatga acgcatcctc ggcgcgttca agaacagcct gatcgtcgcc gccgccacga
     4381 cggtgctttc caccttcatc ggcacggtgg gcgcgtggat gctgcatcgc taccgctacc
     4441 ccttcaaaca attcctggat ttgctggtgc tggtgccgat ggtcatgccg gaggtgttga
     4501 tgggcgtcag cctgctgctg ttttttgtaa gcatgaagat cggcctcggc tacttgacgc
     4561 tgatcatcgc ccacaccacg ttttgttttc ccttcgtgct ggtgggcgtg caggcgcggc
     4621 tgcaggggat cgatcccttc ctggaagaag ccgccatgga tctcggggcg acgccgatcc
     4681 aggcattttg gaaagtcatt gtgccctacc tgatgccggc gatcatttcc ggcgcgctga
     4741 tgtccttcac gctttcgttc gacgaataca tcgtcaccgt tttcaccatg agcgcgcagg
     4801 cgcagacgtt cccggtcacc gtttacggca tggtgaagcg ggggctggat ccgagcctca
     4861 acgcgctgag caccctcttc attctggcca ccgtcattct cgtgctgttc accgaaaccc
     4921 taaaaaagct caaataacat gaaaagaagc gcactcgcac ccaccattgg cgcactggcg
     4981 gcagtcgttc tgctcgccgg ctgcgataaa cccgccacca ccacgcaggc tcccaccccg
     5041 gaaccggcgc cggccgagat ggccgctccc gacgccaccc cggcggtggc ggcgactccc
     5101 gccgccccaa ccaacacgga ggaaaaggtc ctgaacctgt attgttggtc ggaatacgtg
     5161 ccgcagtcgg tcattgacga attccaaaag gcgaccggca tcaaggtcgc ggtggaaaat
     5221 tacgcctcca acgaggaaat gctcaccaag ctcctcgccg gcggcggcac ctacgatctc
     5281 atccagccca gcgaatacgt gatcgaggcg ctcattaaag aaaaactgct cgagccgctc
     5341 gaccatgcgg cgatcccgaa tctcaagaac atcgcgccgg aattccagaa catggcgttc
     5401 gatccgggca acaaatactc cgtgccgtgg atggccggaa cggtcggcat cgtgatcaac
     5461 accggcctcg tcaaggatga catcaaaacc tacgacgacg tgttccaggc gaagaacaag
     5521 ggcaacatcg tcgttctcga cgacgcccgg gaaatcgtga gctgggcgct ggcctcgcag
     5581 ggcatcccga tcaacgacgt gacggatgaa aacctcgaaa aaatcaaacc catcctctcc
     5641 aagtggctcc cgctggtgaa ggtctatgac tccgacagtc cgaaaacctc gatgctgaac
     5701 ggcgacgtcg ccctgggcgt ggtctggagc ggggaaggcg cgctgctgct caacgaggac
     5761 aagaaattca aatgggtgct gcccgtgggc gcgcacctct tcgtggacag cctcgccatt
     5821 ccgaaaggcg ccaagcaccc gaaaaatgcg gaggccttca tcaacttcat cctcctgccg
     5881 gaggtcagca aactcatctc ggcggatttc ccctacctga atccgaacgc tccggcccgg
     5941 gcgctgctca ctccggaaga gcaggcgaat accgccagct tcccgaccgc ggaggaactc
     6001 cagaagatgg aaatcttcac ggacatcgga ccgcaggcca ccaaggtgga cgagatcgtc
     6061 accaccgtca aaagccagta gttcccgtcc atggccacca cagtcattcc acaaggtggt
     6121 ggttccgacc atcaacgcag accgggcttg ggcgcgtggc ttttcctcgc gcccatgctc
     6181 gtctggctga tcctgttcgt cgtcgtcccc tcggtgctgc tcgtcatcat gagcttcgcc
     6241 gagcggggga cgctcgggcg ggtcgtttac aatttttcct tcgacaatta cacccgcgtt
     6301 ttttcgagct ggccgattct gaaaatcttc ctgctctcga tctggtatgc gctcctgacc
     6361 acggtcatct gcgtcatcgt cggttacccc gtcggctatt tcatcggccg gctcccggaa
     6421 cgctggcgaa atctctgcct cacgctcgtc atgatcccct tctggacgag ctttctcatc
     6481 cgcacctacg cgtgggtgag cctgctgagc aaggaaggcc tcatcaatgg cctgctcctc
     6541 tccgcccaca tcattcccag cccgctcggc atgatgtata c
//
LOCUS       DATDAY010000152         2634 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_1304872, whole genome shotgun sequence.
ACCESSION   DATDAY010000152 DATDAY010000000
VERSION     DATDAY010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2634)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2634)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2634
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1304872"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..681
                     /gene="hprK"
                     /locus_tag="VIS74_02930"
     CDS             <1..681
                     /gene="hprK"
                     /locus_tag="VIS74_02930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HEY5792223.1"
                     /translation="PCLVIARNALIPPALLEVASEAEVPVFRTHMITMKFINAATIAL
                     ENEFSPATSEHGSMVDILGVGTLIRGKSGIGKSECVLELIERGYSLVSDDITRFRLAD
                     GRELVGTASELSRFHMEVRGIGIINVAAIFGAGSVRPEKKLDLVVTLKEWHELEDVDR
                     IGLDQDHYEILGIKVPHITIPVKIGRDLGRLIEVAALDTKLKGMGQNSALEFNQRLLQ
                     MMQDQRKK"
     gene            678..989
                     /locus_tag="VIS74_02935"
     CDS             678..989
                     /locus_tag="VIS74_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HEY5792224.1"
                     /translation="MMGLLSRKSSPGDASRNARDLVIQNRNGLHARPAAMFVKISSRF
                     KAEVWVEKDGEKVNGKSIMGLMMLAAGKGSTLHVSAEGSDAEAVLHELDQLIHNRFGE
                     E"
     gene            1099..>2634
                     /gene="ptsP"
                     /locus_tag="VIS74_02940"
     CDS             1099..>2634
                     /gene="ptsP"
                     /locus_tag="VIS74_02940"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HEY5792225.1"
                     /translation="MQGTITPDKTEKRFQGVAIAPGIAHAESLVHWLEDEEIPYRKIA
                     AAEIPGEVARFEGALISTRGELLEIQERIATSIGAKDASIFDAHLLVVEDRTLIDEVL
                     KSLEREQCNIEYIFHKVAEKYIRTLSAIDDPYLRERVVDIEDVARRVIRHLMGKDPRY
                     VSAHDRPHIIVAHTLTPSDTAMMNRSLVLGFATEAGSKTSHTAIMARSLDIPAIAGLH
                     NICEEIDSGDDLLIDGYTGLLILNPSPETLEQYGRIELQKEAVAERLELIRDTVSTTP
                     DGKHIILSANIELPEEMEDVIESGAEGVGLYRTEFLYLNRSKPPDEEEQYETYRTVAE
                     KCSPYSVIIRTLDIGGDKSAESLDLPEEQNPFLGCRAIRFCLDNPEIFKVQLRAILRA
                     SAHGNVRLMYPMVSGESELLQANALLEEAKAELRKEGAPFRENIDVGAMIEVPSAALA
                     ADTLAPHVKFFSIGTNDLIQYAIAVDRVNERIAHLYDPTHPAIIRLIQMIVQAAHRQG
                     IWVG"
BASE COUNT          578 a          843 c          757 g          456 t
ORIGIN      
        1 ccgtgcctgg tcatcgcccg gaatgccttg atcccccccg cgcttctgga ggtcgccagc
       61 gaggcggaag tccccgtttt ccgcacgcac atgatcacca tgaagttcat caacgccgcc
      121 accatcgcgc tggaaaatga gttttcgccc gccacttccg aacacggcag catggtggac
      181 atcctcggcg tgggcacgct catccgcggc aagagcggca tcggcaaaag cgagtgcgtg
      241 ctggaattga tcgaacgcgg ttacagcttg gtcagcgacg acatcacccg cttccgcctc
      301 gcggacggcc gcgaactggt cggcaccgct tcggaactct cccgcttcca catggaggtg
      361 cgcgggatcg gcatcatcaa cgtggccgcc attttcgggg ccggctccgt ccgcccggaa
      421 aaaaaactcg acctggtcgt gaccctcaag gaatggcacg aactggagga cgtggaccgc
      481 atcggactgg atcaggatca ctatgagatc ctggggatca aggtcccgca catcaccatt
      541 ccggtaaaaa tcggccggga tctgggacgc ttgattgaag ttgccgccct ggatactaaa
      601 ttaaagggca tgggacaaaa cagcgccctt gaattcaatc aacgtttgct gcaaatgatg
      661 caggatcaac ggaagaaatg atgggcttat tatctcgaaa atcatccccg ggtgacgcct
      721 cgcgcaatgc gcgggatctc gtcattcaaa atcgcaacgg actccatgcc cggcccgcgg
      781 ccatgtttgt caaaatctcc agccgcttca aggccgaggt ctgggtggag aaggacggcg
      841 aaaaggtaaa tggcaaaagc atcatggggc taatgatgct cgccgccggc aagggctcga
      901 ccctgcacgt ttccgccgaa ggcagcgatg ccgaggcggt gctccacgag ctcgaccagc
      961 tcatccacaa ccgcttcgga gaggaataag cgagggtttc gattgggata ctccccccgg
     1021 cgtttcaatc gaaaacttcg ctttccagat ttccattccg catttgaccg gccgctttcc
     1081 cgatgctacg cctgacgcgt gcagggaacg atcaccccgg ataaaacgga aaaacggttt
     1141 caaggcgttg ccatcgcgcc cggaatcgcg catgccgaaa gcctggttca ttggctggag
     1201 gacgaggaaa tcccgtatcg caaaatcgcc gccgcggaga ttcccggcga agtcgcccgc
     1261 ttcgagggcg cgttgatttc gacccgcggc gaactgctgg aaatccagga gcggatcgcc
     1321 acgtccatcg gcgcgaagga cgccagcatt tttgacgcgc atctgctggt ggtcgaggac
     1381 cgcacgctca tcgacgaggt gttgaagtcg ctcgaacgcg agcaatgcaa catcgaatac
     1441 atcttccaca aggtcgcgga gaaatacatc cgcacgctca gcgcaattga cgatccgtat
     1501 ctgcgcgagc gcgtggtgga tatcgaagac gtcgcccgcc gggtgatccg ccatctcatg
     1561 ggcaaggatc cgcgctacgt cagcgcgcac gaccgcccgc acatcatcgt cgcccacacg
     1621 ctgaccccgt cggacacggc gatgatgaac cgctcgctgg tgctgggatt cgccaccgaa
     1681 gccggcagca aaacgtcgca taccgccatc atggcccggt ccctcgacat cccggcaatc
     1741 gccggcctgc acaatatctg cgaggagatc gacagcgggg acgacctgct gatcgacggc
     1801 tacaccggcc tcctaatcct caaccccagc ccggagacgc tggagcaata cggccgcatc
     1861 gaactgcaaa aggaagccgt ggccgagcgc ctcgagctga tccgcgacac ggtctcgacc
     1921 acgccggacg gcaagcacat catcctctcc gccaacatcg aactgccgga ggaaatggag
     1981 gatgtcatcg aatccggcgc cgaaggcgtg gggctctacc gcacggagtt tctttacctc
     2041 aaccgctcca aaccgcccga tgaggaggag cagtatgaga cttaccggac ggtcgccgaa
     2101 aagtgctcgc cctacagcgt gatcatccgc acgctcgaca tcggcgggga caaatccgcg
     2161 gaaagcctgg atctgccgga agagcaaaac cccttcctcg gctgccgcgc gatccgtttc
     2221 tgcctcgata atcccgaaat cttcaaggtg cagctgcgcg ccatcctgcg cgcctcggcc
     2281 catggaaacg tgcggctcat gtatcccatg gtttccggcg aaagcgaact cctgcaggcc
     2341 aacgcgctgc tggaggaggc caaggccgaa ctccggaagg aaggcgcccc cttccgcgag
     2401 aacatcgacg tcggcgccat gatcgaagtc cccagcgcgg cattggcggc ggacacgctc
     2461 gcgccccatg tgaagttttt cagcatcggc acgaatgatt tgatccaata cgccatcgcg
     2521 gtggaccggg tcaacgagcg catcgcgcac ctctacgatc ccacgcatcc ggccattatc
     2581 cgcctcattc aaatgatcgt gcaggccgcc caccgccagg gcatctgggt tggg
//
LOCUS       DATDAY010000153         8344 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_1216848, whole genome shotgun sequence.
ACCESSION   DATDAY010000153 DATDAY010000000
VERSION     DATDAY010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 8344)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8344)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8344
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1216848"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..616)
                     /locus_tag="VIS74_02945"
     CDS             complement(<1..616)
                     /locus_tag="VIS74_02945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792226.1"
                     /translation="MNSKILHRSYSCTGRFLFFVFSLMISGFFPLSGNAAEPVAGDSC
                     AVSGAFTHAGGPEQTGAGHFLVCNGSAWVSVIDHTASGRTDIQINNDTGSCTTAKTGR
                     LRYNDSTDVWEYCTGSAWSPFEQAGSAVCNVFGQVPGYVCPDGTVFAGFPPNLYEPIF
                     TTRCDAGQSWTGSCTGTRVTKPWNNGNSSGYVTTSQTGLGDGKAN"
     gene            complement(606..890)
                     /locus_tag="VIS74_02950"
     CDS             complement(606..890)
                     /locus_tag="VIS74_02950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792227.1"
                     /translation="MPYRPFLLAVVLPALLFGSFTSSPSAAETDGARTRLAVDEEKNI
                     VHVIVEGREVGRFDKDGLHVEGDITYSGSISDIGPGPDSAGKKQESADEQ"
     gene            1401..1586
                     /locus_tag="VIS74_02955"
     CDS             1401..1586
                     /locus_tag="VIS74_02955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792228.1"
                     /translation="MNLILTHELRNRCERELSVLFLMVSREMAKTARCSPERRNALAS
                     LENISRARMQRMTEGPA"
     gene            1687..2280
                     /locus_tag="VIS74_02960"
     CDS             1687..2280
                     /locus_tag="VIS74_02960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792229.1"
                     /translation="MSAQDIRAALAAAVSPKYRLDRDDWVKVDGEKLYRIVALKDFGD
                     VEAGQRGGYIAGGHNLSQTGNCWVSGLGRVMKNGRVEKNALVCDFAEITDEALLTDDA
                     QASGRGHVGGRARILDCGAVSDRARVVGAAELSDRARAGGDALISGRSTLSGAAFVHG
                     TCVVAGTQNFSGDVDLDGGTWMGSAVPAESVLTVSGP"
     gene            complement(2299..2682)
                     /locus_tag="VIS74_02965"
     CDS             complement(2299..2682)
                     /locus_tag="VIS74_02965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792230.1"
                     /translation="MPAANPNQEGRAKLNFELEEDDPEVSKETVERKIQEISKKSGFT
                     ARASTGAPGEKPLPVLKKAAAIEERPRRRRAKTGRTYPFTTKIKPETYAKICTMADEA
                     TDREGRPVSLAEIIERALESLAEQK"
     gene            complement(2697..3398)
                     /locus_tag="VIS74_02970"
     CDS             complement(2697..3398)
                     /locus_tag="VIS74_02970"
                     /inference="COORDINATES: protein motif:HMM:NF018691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEY5792231.1"
                     /translation="MAKTPVIAFVSPKGGVGKTTATLTLASELVKQLEKPVTIIDADP
                     NYPFKRWVKMGRKPALLNVVFDESEETILENIESARQNSGAVLIDLEGTKNMRVTYAV
                     SQADLVLIPVQGSILDANEAAEAIKLVKRTEQGFNRKIDFAILFTRMPATIVSRNFSD
                     IARQFSDGKIPVLETRLVEREAFKTMFSTGRALHDLTDQHVSGLPNAMLDSYALAEAV
                     IGRINNNRKKVAQAA"
     gene            3738..5216
                     /locus_tag="VIS74_02975"
     CDS             3738..5216
                     /locus_tag="VIS74_02975"
                     /inference="COORDINATES: protein motif:HMM:NF014581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HEY5792232.1"
                     /translation="MRASARLRWERGKHFLGVIDASFGEEKLEDGRLDRFAVGGQAVG
                     IATDMHVVTIAGSRAGKGRAAILPTLLTYPGSVVAIDPKGELASKSALRRAAMGQKCH
                     VLDPFEVAGEGAEALRCSYNPMESIHADNPRIVEDAGLLADAIIQPVPGSTDPHWDES
                     AKNFLEGIILHVGTHSNYKDRRDLVMVNRVLASIKPEYSELLQKEMEENLAPKDAVID
                     AAAAFFGKPDRERESVLSTLRRHTRFLGYESMQNVLGRASISLANLKTAPTTVYLCLP
                     AMRLGTCSRWLRMVINMTLAALEEDLTRPKLPVLLVLDEFAVLGHMKTIEDAAGQIAG
                     LGCKLWPILQDLGQLKALYRERWETFLGNAGIVQCFGNSDLTTLEWISRRLGETTVRT
                     ETKAVLGYVQKFEKNEKGISHAANVHPLLTPEEISRYFGRDDPFLRQLIIIAGYPPLI
                     LQRAYYDRHEIFSKTERAVDRRLEASPRGFVGQKGENIRKRH"
     gene            5359..5877
                     /locus_tag="VIS74_02980"
     CDS             5359..5877
                     /locus_tag="VIS74_02980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792233.1"
                     /translation="MWVFATGANGGSGYFWAFFFVACVVVKTQARGEWVRSRIARRFG
                     STSLVYVGFKDISFGGAIENPTILRSPRGRVGAELRPHERAPMAAAMLQHAPAPNPAA
                     AQSNAWKAAVWQNSFMVYVTYESRIGMQIVRVAEICDQMMALRFVAAINAAEALVQED
                     MTNRTRQRGAWD"
     gene            complement(5807..6802)
                     /locus_tag="VIS74_02985"
     CDS             complement(5807..6802)
                     /locus_tag="VIS74_02985"
                     /inference="COORDINATES: protein motif:HMM:NF024554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3991 and TOPRIM domain-containing protein"
                     /protein_id="HEY5792234.1"
                     /translation="MTRSDELEAFKTQINMVEYAAHLGFEIDRKTSRNWIAMNGPGGK
                     ILISMAPDNHWVYCPVGDKGGGSIIDLDQQHNGGDISDVRKRLRGWINDPSLVMARMA
                     KPPCPVPSSIARDILRVCARLEAMERADGRHPYLQQERRIPGPVLAAARFADRIFIDQ
                     YRNAIFPHRNRAGICGYEIKNRDFTGFAAGGEKGLWSSRTSADDRALVIAETAIDALS
                     YHALKLPELTRYISTAGALNNTQPDLIRSAIEKMPVGSKIILAVDNDEGGDSLAEKIT
                     GIYDGISHPGGALLQDRPARRGADWNDILKSAVNPRPRVAGSDSSCLPAPTLPRH"
     gene            complement(7108..8283)
                     /locus_tag="VIS74_02990"
     CDS             complement(7108..8283)
                     /locus_tag="VIS74_02990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792235.1"
                     /translation="MAYDSKKPLTPAVPRDFVTAPIFAATKQTEPRTIIVNGVRWIIG
                     DEHPTKHEPSPAFDIRHGRACLTALSFRDRMQNGRDINFSMNEFCHRYAQSRGGRYSR
                     DILDILFDLRETWVRRISPDGRIERFTIIGEIKIDERPIRRRDDARLLETQGELWLDR
                     VSLSPEFFGLLQQWETLAKIRLDVLNGLTSPTAQAIYTFLPSRAVHHDEGQPFAIRLT
                     NLLEQISAPVSPHKSVRKRLFTQNTHSILSQLDGAQIMNGTLRASIDETKDGKDFNLL
                     AWAENVREPRPLPAFSAPNSKLLDMWLAKGRSKKEFDRRMKRRAPLSGYAEELLEKAK
                     VKVDDGNRAFFEIASGFLSEDRFHGILAEAKGDTLEGDPGKNPTGRLIHRLMKAIEG"
BASE COUNT         1515 a         2298 c         2726 g         1805 t
ORIGIN      
        1 tgtttgcttt gccatccccc agcccggtct ggcttgtggt cacatagcca gatgagttgc
       61 cgttattcca cggcttcgtt acccgcgttc ccgtgcagct tcccgtccag ctctgccccg
      121 cgtcacaacg ggtggtgaaa atgggctcat acaggttcgg cggaaacccg gcgaacaccg
      181 tgccgtcagg gcagacatag ccgggaactt gtccgaagac attgcagaca gcgctcccgg
      241 cctgctcaaa agggctccag gcgctccccg tgcagtattc ccacacgtcg gttgaatcgt
      301 tgtagcgcag gcggcctgtt ttggccgtgg tgcaggagcc ggtatcgttg ttgatttgga
      361 tatcggttcg gcctgacgcc gtatggtcga tgacggatac ccaggcgctg ccgttgcaga
      421 caagaaaatg ccccgcgccc gtctgctccg gcccgcccgc atgggtgaac gcgccagaga
      481 ccgcgcagga atcgccggcg accggctcgg ccgcgtttcc tgaaagagga aagaaacccg
      541 aaatcataag gctgaagacg aaaaacagga accggcccgt acaggaataa gacctatgaa
      601 gaattttact gttcatcggc ggactcctgc ttttttccgg cggagtccgg tccggggcct
      661 atatcggaaa tcgaaccgct ataggtaata tcgccctcaa catggaggcc gtctttgtcg
      721 aaccgtccga cctcgcggcc ctcgacgata acgtggacga tattcttttc ctcatcgacg
      781 gccaggcgcg tccgcgcgcc gtccgtttcg gcggcggagg gagaagaagt aaaagaacca
      841 aaaaggagcg ccggaagaac cacggcaagc aggaaaggcc tgtaaggcaa cctcaggggg
      901 cggatttctg cggagcggtt accccccccc agatttatgg taaattacgg tctgcattgt
      961 ttctattccc ttttttaatg taattatttt tcgccatggt acatgctttc gtatcaaaag
     1021 ttaaggccct aacctggtca tgagccggtg aaccgttccc agatgccagc gcccgccctt
     1081 ggcggtcggg caggcgtccc cctcctgtcg tcatcatcag aaccgctgaa agagcgcgct
     1141 ggcgcatcag ttccagggca gtgcaggcgt caggaagttt cctttccccc tcccctgccc
     1201 attcccgccc tggtcctttt caatcgtccg atgctgcccg aatccggcca gagagatcaa
     1261 cggcctgccc atcagaggat ctgcgttggc cgtttccccc tggaaagtag gttgtccggg
     1321 gtgatcttct gcccgtctcc ggggctgctt cttcctcatg aaaggcccca atggcggggc
     1381 ctgtagcata ggaggaaaaa atgaacctga tcctgaccca tgaactgcgc aaccggtgcg
     1441 agcgcgaact ttcagtgttg ttcctgatgg tgtcgcgcga gatggcgaag acggcgcgct
     1501 gctcgcccga gcgccggaac gccctcgcct ccctggagaa catcagccgg gcgcggatgc
     1561 agcggatgac ggagggcccg gcttaggccg gacccgttct tcgcgtttac ggtcgcgcca
     1621 tgcgtccgca tgcagttgac ggaatgttaa tcccctcccc cgcagaatgg tctgcggagg
     1681 atgggcatga gcgcacagga tattagggcc gccctggcgg cggcggtatc gccgaaatac
     1741 cggctcgatc gcgacgactg ggtgaaagtc gatggcgaga agctctaccg gatcgtggcg
     1801 ttgaaagatt tcggcgatgt cgaggcgggg cagcggggcg ggtatatagc gggcgggcat
     1861 aatttatcgc agaccggcaa ttgctgggtt tccgggctcg gccgcgtgat gaaaaacggg
     1921 cgggtggaaa agaacgccct ggtgtgcgat ttcgcggaaa tcacggatga agccctgctg
     1981 accgatgacg cgcaggcgag cggtcgcggc catgtcgggg gtcgcgcgcg gattctggat
     2041 tgcggcgcgg tttccgaccg ggcgcgagtc gtcggcgcgg cggaattatc ggacagggcg
     2101 cgcgccggcg gcgacgcgct gatatcgggg cgcagcacgc tgtcgggcgc ggcgttcgtg
     2161 catggaacct gcgtggtcgc cgggacgcag aatttcagcg gggacgtcga tctggatggc
     2221 gggacgtgga tggggagcgc cgttccagct gaatccgtat tgacggtttc agggccgtga
     2281 agtcccctcc cctcctcctc atttctgctc tgcgaggctt tcaagagccc tttcaataat
     2341 ctcggcgagg ctgacaggcc tcccttccct gtccgtggcc tcatcggcca ttgtgcagat
     2401 tttggcgtag gtctcgggtt tgattttggt ggtgaacgga taagtccgcc cggtcttggc
     2461 gcgccggcgg cgggggcgtt cttcgatggc cgcggctttt ttgaggacag gaagcggttt
     2521 ttcgccaggc gcgccggttg aggctctggc tgtaaagccg gattttttac tgatttcctg
     2581 gattttgcgc tcgacggttt ccttcgagac ctcgggatcg tcctcctcaa gctcgaaatt
     2641 gagttttgcg cggccttcct gattagggtt tgctgctggc atatccgttt cctcctttag
     2701 gccgcctggg ccactttttt gcggttgttg ttgatgcggc cgataacggc ttcggccagg
     2761 gcgtatgaat ccagcatggc gttcggcagg ccgctgacat gctggtctgt cagatcatgg
     2821 agcgccctgc ccgtcgaaaa catggttttg aaggcctccc gctcgacgag ccgggtttcg
     2881 aggaccggaa ttttcccgtc cgagaactgg cgggcgatat cggagaaatt tcgggaaacg
     2941 atggtggccg gcatccgggt gaacaggatg gcgaagtcga ttttgcggtt aaagccctgc
     3001 tcggtgcgct tgaccagctt gatggcctcg gcggcttcgt tggcgtccag aatcgaaccc
     3061 tgcacgggga tgagaacaag atcggcctgg ctgacggcat aggtcacgcg catgttcttg
     3121 gttccctcga gatcgatcag gacagccccg gaattctgcc tggcgctttc gatgttttcg
     3181 agaatcgtct cttccgactc atcaaaaacg acgttcagaa gcgccggttt gcggcccatc
     3241 ttgacccatc ttttgaaggg atagttcggg tccgcgtcga tgatggtgac cggcttttca
     3301 agttgtttaa caagctcgct ggccagtgtg agcgtcgcgg ttgtcttgcc gacgccgcct
     3361 ttgggcgaaa caaatgcaat gacgggtgtt tttgccattc tttcttcctt cctgtggatt
     3421 gcggatatct ggaacaggta ttgaaaacct atggccgatt ataaacaagt ttaaatatta
     3481 cagcaagcta tagtattgcc gaaatctatc tgaaagcttt ccgctggatt gcggaaagct
     3541 ttcagcaata ccttttgttg ccttaaggca ggcctggcgg attttcggcg ggggcagcag
     3601 ggaaaaagaa ttttcagcgc gccctgtctg atgcacattg cgcgattcct gacgatctcc
     3661 atagcgacct gccgcgcggc gggcataagg cgggggaggg ggaaagccct tccgcgtcat
     3721 tcacgccgac ggacagcgtg cgggcctccg cgcgcctgcg gtgggagagg gggaaacatt
     3781 ttctgggggt catcgacgct tcgttcggcg aagaaaagct ggaagacggg cggctggacc
     3841 ggttcgcggt cggcgggcag gccgtgggca tcgcgacgga catgcacgtc gtcaccatcg
     3901 cggggagccg ggccggaaaa ggccgcgcgg cgattttgcc gacgcttctg acatatccgg
     3961 gctcggtcgt cgccatcgat cccaaaggcg agctggccag caagagcgcc ctgcgccgcg
     4021 ccgcgatggg ccagaaatgc catgtgctcg atccgttcga ggtggcgggg gagggggcgg
     4081 aggcgctgcg ctgctcttac aatccaatgg agtcaattca cgcggacaat ccgcgcatcg
     4141 tggaggatgc ggggctgctc gccgacgcga tcatccagcc ggttccgggc agcacggacc
     4201 cgcactggga cgagtcggcg aagaattttc ttgaaggaat catcctgcat gtcggaacgc
     4261 attcaaatta taaagaccgc cgcgacctgg tcatggtgaa cagggttctc gcatcgataa
     4321 agcctgagta ttccgaactt cttcaaaagg aaatggaaga aaatctggcg ccgaaggacg
     4381 ccgtcatcga cgcggcggcg gccttcttcg gaaagccgga ccgcgagcgg gagagcgtgt
     4441 tgtcgaccct gcggcggcat acgcggttcc tgggctatga gagcatgcag aacgtgctgg
     4501 gccgcgcgtc gatatcgctt gcgaatctca agacggcgcc gacgacggtt tatctctgcc
     4561 tgccggcgat gcgtcttggc acctgttcgc ggtggctgcg catggtcatc aacatgacgc
     4621 tcgccgcgct cgaggaggat ctgacgcgcc cgaaactgcc cgtgctgctg gtgctggatg
     4681 agttcgcggt gctgggccac atgaagacga tcgaggacgc cgcggggcag atcgccgggc
     4741 tggggtgcaa gctgtggccg atattgcagg atctcggcca gctcaaggcg ctctacaggg
     4801 agcgctggga aaccttcctc ggcaatgccg ggatcgtgca atgcttcggg aattcggacc
     4861 tgacgacgct tgagtggata tccaggcgcc tcggcgagac gacggtgcgc accgaaacga
     4921 aagccgtgct gggctatgtg cagaagtttg aaaaaaacga gaaagggatc agccacgcgg
     4981 ccaacgtgca tccgctgctg acgccggaag agatttcccg gtattttgga cgtgacgatc
     5041 cgtttctgcg gcagttaatc atcattgcgg gatatccacc cctgatcctg cagcgggcct
     5101 attacgaccg ccatgagatt tttagcaaaa ctgagcgcgc tgttgacagg aggcttgagg
     5161 cttccccgcg cggcttcgtg ggacaaaaag gagaaaatat ccggaagcgg cattgactca
     5221 tggccgcatc tttacgcgtg cgaggccggc attatcgtgg aggccgcggg gtatttgccg
     5281 ccgggaacct atataaaaag ggtcggattt atcgagttcg tcgcagtgct gggagcgctc
     5341 gtctcgtttt ttccatcgtt gtgggttttc gccaccggcg cgaacggggg gagcggttat
     5401 ttctgggcgt ttttttttgt ggcctgcgtc gttgtcaaga cgcaggcgag gggggaatgg
     5461 gtccgcagcc ggatcgcgcg gcgctttggg tcgacgtcac tggtctatgt cggatttaag
     5521 gatatcagct ttggcggcgc gattgaaaat ccgacgatcc tgcgcagccc gcggggaaga
     5581 gtgggggcgg aactcaggcc gcatgagcgc gcgcctatgg cggcggcgat gctgcagcac
     5641 gcccccgcgc cgaacccggc cgctgcgcag tcaaacgcct ggaaggcggc ggtatggcag
     5701 aattccttca tggtttatgt gacctacgaa tcccggatcg gcatgcagat cgtccgcgtc
     5761 gccgaaattt gcgaccagat gatggcgctc cggtttgtcg cggcgatcaa tgccgcggaa
     5821 gcgttggtgc aggaagacat gacgaatcgg acccggcaac gcggggcctg ggattaaccg
     5881 cagatttaag gatatcgttc cagtcggcgc cgcgccgggc cggacggtcc tgcagcagcg
     5941 cgccgccggg gtgagagatg ccgtcataga tgccggttat tttttcggca aggctgtcgc
     6001 cgccctcgtc gttatcgacc gcgaggatga tcttgcttcc gaccggcatc ttctcgatgg
     6061 cggagcggat caggtccggc tgggtattat tcagcgcgcc cgccgtgctg atataacggg
     6121 tcagttccgg cagtttgagc gcgtggtagc tcaaagcgtc gatggcggtt tcggcgatga
     6181 caagcgcgcg gtcgtccgcg ctggtgcggc tgctccacaa tcctttttcg ccgccggcgg
     6241 cgaagccggt gaaatccctg tttttgattt cataaccgca gattccggcg cggttcctgt
     6301 gcggaaaaat ggcattgcgg tactggtcga tgaaaatccg gtcggcaaaa cgcgcggcgg
     6361 cgaggacagg gccggggatg cggcgctcct gttgcagata ggggtgcctg ccgtcggcgc
     6421 gctccatcgc ttccagccgg gcgcagacgc gcagtatgtc cctggcgatg gacgagggga
     6481 ccgggcaggg cggttttgcc atgcgggcca tgacaaggga aggatcgttg atccagccgc
     6541 gcaggcgttt gcgcacatcg ctgatatcgc cgccgttatg ctgctggtcg aggtcgatga
     6601 tgctgccgcc gcctttgtct ccgacggggc aataaaccca gtgattgtcc ggagccatgc
     6661 tgatcaggat cttgccgccc ggcccgttca tcgcaatcca gttgcgggac gttttccggt
     6721 cgatttcaaa gcccagatgg gccgcgtatt cgaccatatt gatctgcgtc ttgaaggcct
     6781 cgagttcgtc gctccgggtc attttctttt ttcctgccgc cccttgtttt caaattctgc
     6841 acggagaggt tttaatattc gctgaaaatc atgcgggaat ttttttgaaa agggaatggt
     6901 gaaatattta gggggaagtt aaatggggag gtgtgagtaa gtttcgtaag tggctgaaga
     6961 tcaaggggaa atgggaaagg cgtttgttta gctgtcccaa ccctttgttt agctgtccca
     7021 acccttacgg gcggtttttg tttagctgtc ccaacccttg ggcggcgcgt tttgcgaatc
     7081 gcccgccgaa tcgttttcgg cgtcggtcta accttcgatt gctttcatga ggcggtgaat
     7141 caggcggccc gtcggattct ttccggggtc gccctccagc gtgtcgccct tcgcctcggc
     7201 gaggatgcca tggaagcggt cttcgctgag aaagccgctg gcgatctcga agaaagcgcg
     7261 gttgccgtca tcgactttaa cctttgcctt ttccagcagc tcctcggcgt agccggataa
     7321 aggcgcgcgg cgcttcatgc ggcggtcgaa ttccttcttt gaccggccct tcgccagcca
     7381 catatcgagc agtttggaat tcggcgcgga aaatgccggg agggggcggg gttcacggac
     7441 attctccgcc caagccagca ggttgaagtc cttaccgtcc ttcgtttcgt ctatcgatgc
     7501 gcgcagcgtg ccgttcatga tctgcgcgcc gtcgagctgc gacaggatgg agtgggtgtt
     7561 ctgcgtgaaa agccttttac ggacggattt gtggggcgat accggggcgc tgatctgctc
     7621 cagcaggttg gtcaggcgga tcgcgaaggg ctggccctcg tcgtgatgga cggcgcggct
     7681 gggcaggaag gtatagattg cctgcgcggt cgggctggtg agtccgttga gaacgtcgag
     7741 gcggattttc gccagggttt cccattgctg cagaaggccg aaaaattccg gggagaggct
     7801 tacgcggtcc agccagagtt cgccctgcgt ttcaagaagg cgggcgtcat cgcgccgccg
     7861 gatggggcgt tcgtcgatct tgatttcgcc gatgatggtg aagcgttcaa tgcggccgtc
     7921 cgggctgatg cgcctgaccc aggtttcgcg caggtcgaac aggatgtcga gaatgtcccg
     7981 gctgtagcgg ccgccgcggc tctgcgcgta gcggtggcag aattcgttca tgctgaaatt
     8041 gatgtcccgc ccgttctgca tccggtcccg gaacgaaagc gcggtcaggc aggcgcggcc
     8101 atgccggatg tcgaaggcgg gggagggttc gtgttttgtc gggtgctcgt cgccgatgat
     8161 ccaccggacg ccgttgacga tgatcgtgcg cggctcggtc tgcttcgtcg ccgcgaagat
     8221 aggcgccgtc acgaagtcgc gcgggacggc gggggtcagg ggctttttgg agtcgtaagc
     8281 catggcggtg tcagtgctgc tccgccgccg ctgcagtcgc cacggtgctg ccgcgcggga
     8341 caat
//
LOCUS       DATDAY010000154         2687 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_67558, whole genome shotgun sequence.
ACCESSION   DATDAY010000154 DATDAY010000000
VERSION     DATDAY010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2687)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2687)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2687
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67558"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..291
                     /locus_tag="VIS74_02995"
     CDS             <1..291
                     /locus_tag="VIS74_02995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792236.1"
                     /translation="YYGSNKQKSLYLWVPLTRPEYESLEAALRKREKAVSVPQYLQSV
                     YNKEVKELLKPAGSYENLDAIFEQKKDGEVLEALKLKMRARVEEFLSQTKPA"
     gene            complement(379..1644)
                     /locus_tag="VIS74_03000"
     CDS             complement(379..1644)
                     /locus_tag="VIS74_03000"
                     /inference="COORDINATES: protein motif:HMM:NF015177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalase family glycosidase"
                     /protein_id="HEY5792237.1"
                     /translation="MSAQIRTLREQLSGDYQGMFRPAGDALTHPFLTPGSRQYADVLW
                     DWDSWLSDVALRQILLEASDPEKTAEALDYEKGCVLNFLEVARSRPRPDGWIPINITR
                     AGCTPPDDVYAHNMHKPCLAQHAAFLVRESGGDAEWLRDYAYGLESFVNNYRNHHRHE
                     CGIYFWQDDGGIGVDNDPCTYGRPPRSSGSIFLNCLMYRELIALVYVLEQLSLHEVAA
                     QYQDDARELAEAIQKYCWDERDGFFYSVDLNLAPVERGRWRLHAGQPRDWPCLIQRIG
                     VWSGFLALWAGLATPEQAKRIVKEHYWDTRTFCAPYGVRTLSKLEKMYNLRASGNPSS
                     WLGPIWGISNYLTFRGLVQYGFTAEARDLAEKTIKLFARDIERFGALHEYYQPENGEP
                     ILNRGFQNWNYLVLNMAAWFEGRPFVAEF"
     gene            complement(1913..2155)
                     /locus_tag="VIS74_03005"
     CDS             complement(1913..2155)
                     /locus_tag="VIS74_03005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792238.1"
                     /translation="MKCQIYFAGKISAVEKSKHTDLPPHLPLHHYTANTFYFTTQGEM
                     RSGNRGAIFEVGGGQAVKVTLSCHNFNNKGLGEFTR"
     gene            2219..>2687
                     /locus_tag="VIS74_03010"
     CDS             2219..>2687
                     /locus_tag="VIS74_03010"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEY5792239.1"
                     /translation="MATITGTNGNDTLHGTSGDDLINALGGTDILYSDAGSDTINGGA
                     GINYYNADYGDRTSGLTMTYDSASGNGQIVVGGEVDAFTAIARFGYFYGTAFNDSIVG
                     TADADEIHGNAGNDTLSGGAFYDTLLGGDGNDVLRGGVYEDSLYGGNGNDLLKG"
BASE COUNT          545 a          730 c          867 g          545 t
ORIGIN      
        1 tactacggca gcaacaagca aaagtcgctt tatctctggg tgcccctcac gcggccggaa
       61 tacgagtccc tggaggcggc gctgcggaag cgggaaaaag cggtttccgt gcctcagtat
      121 ttgcaatcgg tctataacaa ggaagtaaag gagctgttga agcctgcggg ttcctacgag
      181 aaccttgatg ccatcttcga gcaaaaaaag gacggcgaag tgctggaagc cctcaagctc
      241 aagatgcggg cgcgcgtcga ggaatttctg agccagacca agccggcgta agccggcaca
      301 agagcgaagc gacggacagc tcatcgagct gtccctgcct tcaaatggta gggcgggttc
      361 aatgaacccg ccgtgactct aaaactcggc gacgaacggc cggccctcga accacgcggc
      421 catgttcagc accaagtaat tccagttctg aaatccgcgg ttgaggatcg gttcgccgtt
      481 ttccggctgg taatactcgt gcagcgcgcc gaaacgttcg atgtcccggg cgaagagctt
      541 gatggttttt tccgccaggt cgcgggcttc cgcggtgaat ccgtattgca ccaaaccgcg
      601 gaaggtcagg tagttggaga ttccccagat cgggcccagc caggaggagg ggtttcccga
      661 agcgcggaga ttatacattt tttccagttt ggaaagcgtc cgcacgccgt agggggcgca
      721 gaaggtgcgg gtgtcccaat aatgttcttt gacgatgcgc ttggcttgtt ccggggtggc
      781 gagaccggcc cagagggcga ggaacccgga ccagacgccg attcgttgaa tgaggcaggg
      841 ccaatcccgg ggctgcccgg catgcaggcg ccaccgccct cgttccaccg gcgccaggtt
      901 cagatccacg ctgtagaaaa aaccatcccg ctcgtcccag caatatttct ggatggcttc
      961 ggcgagttcg cgggcgtcgt cctggtattg cgccgccact tcgtgcaggg aaagctgctc
     1021 cagcacatac accagcgcga tgagttcgcg atacatcagg cagttcagga aaatggagcc
     1081 gctgctgcgc ggcgggcggc cgtaggtgca gggatcgttg tccacgccga tgccgccgtc
     1141 gtcctgccag aaataaatgc cgcattcgtg ccggtggtgg ttgcggtaat tgttgacgaa
     1201 cgactcgagc ccgtaggcgt agtcccgcag ccattcggcg tcgccgccgc tctcgcgcac
     1261 gaggaaggcc gcatgctggg ccaggcaggg tttgtgcatg ttgtgcgcat agacgtcgtc
     1321 gggcggagtg catccagccc gggtgatgtt aatgggaatc cagccatccg gccggggccg
     1381 ggaacgcgcg acttccagga aattgagcac gcagcctttt tcgtaatcca gggcttccgc
     1441 ggttttttcg ggatcgctgg cttcgagcag aatctgccgc agggcgacat cgctcagcca
     1501 cgaatcccaa tcccagagca catccgcgta ttggcggctg ccgggggtga ggaatggatg
     1561 ggtgagggcg tccccggccg gccggaacat gccttgataa tccccggaaa gttgctcgcg
     1621 cagagtgcgg atttgcgcgc tcaaggcaga atcggagtgg gcggattccg gaaagcgggg
     1681 ggccggcgcc gcttcttcaa gcaaaggagg tttttctaat ttcatggatg gggtaagcgt
     1741 aacgctttcc gcagggtcag cgcgaaagaa gaggccttta gtataagaat tgccccgttc
     1801 tgtcaacttc gtcattcgtt tctttttctg cggggaactc cctggttggg gaagggggcg
     1861 cggacgctcg ggctgggttc ccggcggccc ttgcgaagct gccttcgtcc gctcagcggg
     1921 taaactcacc caggcccttg ttgttaaagt tgtggcagct aagggtgacc ttgacggcct
     1981 ggccgccgcc gacttcgaag atcgctccgc ggttgccgct gcgcatctcg ccctgcgtgg
     2041 tgaagtagaa ggtgtttgcg gtgtaatggt ggagcggaag gtgaggcggg agatcggtgt
     2101 gtttcgattt ttcaacggct gatattttgc cggcgaaata aatttggcat ttcactggaa
     2161 aatggctgat ttccagtggt aggctttggc aagtctcttt ccccccccct taaaacttat
     2221 ggcaacgatc accggaacca acggcaacga cacgctccac ggaacgagtg gggatgactt
     2281 aatcaacgca ttgggaggca cggatatcct ttactccgat gcgggcagcg acacgatcaa
     2341 cggcggcgcc gggattaatt attacaacgc cgactacggc gaccggacca gcgggctgac
     2401 gatgacctac gattcggcat cgggcaacgg gcagatcgtg gtgggcggcg aggtggacgc
     2461 atttaccgcc atcgcgcggt tcggctattt ttacggaacg gcgtttaatg actccatcgt
     2521 gggcacggcg gacgcggatg agatccacgg caacgcgggc aacgacactc tctccggcgg
     2581 agccttttac gatactttgc ttggcggaga cggcaacgat gtgctccggg gcggcgtcta
     2641 tgaagactcg ttgtatggag gaaacggcaa cgacttgctg aaaggag
//
LOCUS       DATDAY010000155         4260 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_817750, whole genome shotgun sequence.
ACCESSION   DATDAY010000155 DATDAY010000000
VERSION     DATDAY010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 4260)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4260)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4260
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_817750"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..287)
                     /locus_tag="VIS74_03015"
     CDS             complement(<1..287)
                     /locus_tag="VIS74_03015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792240.1"
                     /translation="MNPVPLCLLLLVISVLPLRAADTAYEALSVVRNKLTADGLKNVI
                     EVKGERGADQPPAWTILMNDSSARGGIRELVVSKGAVVSERTPLGGFSGAG"
     gene            complement(332..1117)
                     /locus_tag="VIS74_03020"
     CDS             complement(332..1117)
                     /locus_tag="VIS74_03020"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEY5792241.1"
                     /translation="MNARSLPVAPVTKWLMAACVAGFAGQVLLGTGQNGMVEVFLGLS
                     GRGLAAGFWWQPVTYLFLHGGVLHLLMNMVGLWFAGREVEYTFGRARYLATFFAGGIL
                     GGFLQVALAGNPDIPLVGASASVCAVLLAFTAIYPNLPITALLFFVVPVQLKAKYLGY
                     LVLGISLVLGVTGIQPGIGHYAHLGGGLAGLLAACWLGFGRGFLPRFSFLRRRSVDAG
                     GLTVPPPEAENLDAILHKVIRHGLHTLTPEERAVLERWNRRRR"
     gene            complement(1114..1983)
                     /locus_tag="VIS74_03025"
     CDS             complement(1114..1983)
                     /locus_tag="VIS74_03025"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEY5792242.1"
                     /translation="MKNNKVLVILNPAARGDRARLLKQRIESLSLRAVIRTTRAAGDA
                     EALAERGVEQGFETIVAAGGDGTVNEVVNGIGESGVPLGILPVGTMNVFAMELGIPPG
                     LEEAWRVIDQGRVREIDLPVCNGEFFVQLGGVGLDAEVVKATTVESKKALGPLSYVLS
                     LAQISAQVPPRLHITAEGVGKREGRFVLVGNGRFYGGPLVLFKDARVDDGLLDVVIFK
                     NQSPWDVVRYVQAIVFGSHPALPDVEYFQTRAIRVEGHESIPYEVDGELSGTLPCEFS
                     FAESKLRVRAPAK"
     gene            2021..3445
                     /locus_tag="VIS74_03030"
     CDS             2021..3445
                     /locus_tag="VIS74_03030"
                     /inference="COORDINATES: protein motif:HMM:NF024893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HEY5792243.1"
                     /translation="MHRISKLILTVAASLLGLVVMSFVGANLYLQSSEVQNRIRTDVE
                     RAIGAPLQVYGTSLTPWSGLTLSNLAVFDPQKPDTLLFKARSMNAKLNWWLLLSGRVE
                     VTSVTLNEPLLIVESGRGITLPSPRQEIPGGAPPPSAPVEPAPATNAISSAEPKTPPR
                     HSRPLRIAVKRFALRNGMVVVKDPNNRHLVEIHGLSADSQILSETDVRGTLQAEEIFL
                     AQHFVIRHAKADYTFSGEQLNLSPITAELAQGAIKGIANVQTVTREASIALTLDQAQV
                     PTLLEEAGLPSEGSRGVLAGNLELAGILGKDETYAGQGAFSLTSGTLQPLDFIRKVGE
                     VLQIDELQLLQLETASLRVAIGHRRTQIEELVLKSKNLILSGTGPVRFDGRLNLNAQL
                     KINEKLQRNLQGLLNRNFTESDLPGYKQLTFKITGTLSKPNTDDLLEKLTGIRGLSGE
                     VGNFLQGLFRKPKPSPSPKSSTTP"
     gene            3452..4012
                     /gene="rsmD"
                     /locus_tag="VIS74_03035"
     CDS             3452..4012
                     /gene="rsmD"
                     /locus_tag="VIS74_03035"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:TIGR00095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEY5792244.1"
                     /translation="MRIVAGTAGGIQLIRPPEHVRPTMDRVRGAIFSSLGEKVADATV
                     LDLFAGSGGLGLEALSRGAARTTFVDSDSKCGQIIRQNLTRCHLAAAVQNMDVFRFLE
                     LYAEENSFDLIFADPPFTKKPGDFDFATALLTNPHLKRALHPEGIFILEKLYSSPRPA
                     DLGGWQVRREKRYGECEVLFLARPEL"
BASE COUNT          867 a         1498 c         1165 g          730 t
ORIGIN      
        1 ctcccggccc cgctgaatcc acccaggggg gtgcgctccg aaaccaccgc gcccttgctg
       61 accacgagct cccggatgcc gccgcgggcg gaggaatcat tcatcaaaat ggtccaggcc
      121 ggcggctggt cggccccgcg ctcgcccttg acctcgatga catttttcaa cccgtcggca
      181 gtcagcttgt tccgaaccac ggaaagcgcc tcgtaggcgg tatccgccgc ccgaagcggg
      241 aggactgaaa tgacgagcag gagaaggcag agcgggaccg ggttcacgag taaaagctta
      301 ccatattctc cagcgggatc aatccgaagc attaacgccg gcggcggttc cagcgctcca
      361 aaaccgcccg ctcctcgggg gtcagcgtat gcaggccgtg ccggatcacc ttgtggagga
      421 tggcgtccag attttccgcc tccggcggcg gcaccgtcaa accgccggca tccacggaac
      481 gcctccgcag gaaggaaaac cgcggaagga atccgcggcc aaatcccagc cagcaggcgg
      541 ccagcaggcc ggcaagcccg ccgccgagat gcgcgtaatg cccgatgccg ggctgaatcc
      601 ccgtcactcc cagcaccagg gaaatcccca gcacgagata cccgaggtat ttcgccttca
      661 attgaaccgg caccacgaaa aacagcagcg ccgtgattgg cagattcgga taaatggccg
      721 tgaaggccag cagcaccgcg caaaccgacg ccgaagcgcc aacgagggga atgtccggat
      781 ttcccgccag cgccacctgc aggaatccgc ccaggattcc tccggcaaaa aaggtggcca
      841 gataccgcgc gcgcccgaag gtgtattcca cctcgcgccc ggcaaaccag agcccgacca
      901 tgttcatgag caaatgcagc actccgccgt gcagaaagag atacgtcacc ggctgccacc
      961 aaaacccggc ggccagtccg cggccgctga gccccaggaa gacctccacc atcccattct
     1021 gccccgttcc gagcaggact tgcccggcga atcccgccac gcaagcggcc atcagccatt
     1081 tggtgaccgg agccaccggc agggagcggg cattcatttg gccggggcgc gcacgcgcaa
     1141 tttggattcc gcaaaggaaa attcgcaggg cagcgtgccg gacaattccc catccacttc
     1201 gtagggaatg gactcatgcc cttccacccg gatcgcgcgg gtttgaaaat actccacatc
     1261 gggcaacgcg ggatggctgc cgaaaacgat ggcctgaacg taacggacca catcccaagg
     1321 ggattggttc ttgaagatca cgacatccag caggccgtca tccacccggg cgtccttaaa
     1381 gagcacgagc ggcccgccgt agaaccggcc gttgccgacc agcacaaagc gcccctcgcg
     1441 cttccccact ccttcggccg tgatgtggag ccgcggcggc acctgcgccg agatctgggc
     1501 cagcgagagc acgtaactca atgggccgag cgcctttttg gactccacgg tggtcgcttt
     1561 caccacctcc gcgtccaggc cgaccccgcc cagctgcacg aagaattcgc cattgcaaac
     1621 cggcagatcg atctcgcgca cccgcccttg atcgatcacc cgccaggcct cttccagccc
     1681 cgggggaatc cccagctcca tggcgaagac gttcatggtt cccaccggca aaattcccag
     1741 cggcacgccg gattcgccga tgccgttgac gacctcgttg accgtgccat ccccgcccgc
     1801 ggcgacgatg gtttcaaacc cctgctcgac cccgcgttcg gccagcgcct cggcgtcccc
     1861 ggcggcgcgg gtggtgcgaa tgacggcccg cagggacagg ctctcgatcc gttgtttgag
     1921 caggcgggcc cgatctcccc gcgcggcggg atttaaaatg acaaggactt tattattctt
     1981 cacgcttcac ctcaggccaa gagttgcgta gcttcccgcc gtgcacagaa tttccaagtt
     2041 aattcttact gttgccgcat ccctcctcgg attggtggta atgagtttcg ttggagccaa
     2101 tctctacctg caatccagcg aagtccagaa ccgcattcgc acggatgtcg agcgggcaat
     2161 cggcgctccc ctccaggttt acggcaccag cctcaccccc tggtcgggcc tcacgctcag
     2221 caacttggcg gtcttcgatc cgcaaaagcc ggacacgctg ctcttcaagg cccgcagcat
     2281 gaacgcgaag ctgaactggt ggctgctgct gagcgggcgc gtcgaggtga cttccgtcac
     2341 gctgaacgag ccgctgctga tcgtggaatc cggccgcggc atcacccttc cctccccccg
     2401 gcaggaaatc cccggcggcg ccccgccgcc gtccgcgccg gttgaaccgg ccccggcgac
     2461 gaacgccatt tccagcgccg agcccaaaac cccgccccgt cactcccggc ccctgcgaat
     2521 cgcggtgaaa cggtttgcct tgcgcaacgg catggtggtc gtcaaagacc cgaacaaccg
     2581 ccatctggtg gaaatccacg gactgagcgc cgattcccaa atcctctccg aaaccgacgt
     2641 ccgcggcacc ctgcaagccg aggaaatctt cctggcccag cacttcgtca tccgtcacgc
     2701 caaagccgat tacacattct ccggcgagca actgaatctc tcccccatca ccgccgaact
     2761 cgcccagggc gcgatcaagg gaatcgccaa tgtccagacc gtcacgcggg aagcgtcgat
     2821 cgcgctgacc ctcgaccagg cccaggtgcc cacgctgctg gaggaagccg gcctgcccag
     2881 cgaaggaagc cgcggagtcc tggccggaaa tctggaactg gccggcatcc tgggcaagga
     2941 cgaaacctac gccggccagg gcgccttttc gctgacctcc ggcaccctgc agccactgga
     3001 tttcatccgg aaagtcggcg aggtgctgca aattgatgaa ttgcaattgc tgcagctcga
     3061 aaccgcctcg ctgcgcgtgg cgatcggcca ccgccgcacc cagatcgagg agcttgtcct
     3121 caaatccaaa aatctcattc tcagcggaac gggcccggtg cgattcgacg gccgcctcaa
     3181 cctcaacgcg cagttgaaga tcaacgaaaa actccagcgc aatctccagg gattgctcaa
     3241 ccggaatttc accgaatccg acctccccgg ttataaacaa ctgaccttca agattaccgg
     3301 cacgctttcc aaacccaata ccgacgatct gctcgaaaaa ctcaccggca tccggggcct
     3361 gagcggcgag gtgggaaatt tcctccaggg actcttccgc aaacccaaac cgagtccctc
     3421 gcccaagtcc tccaccaccc cctgagctcg catgcgcatc gttgccggaa ccgcgggcgg
     3481 cattcaactc attcgcccgc ctgaacacgt ccgccccacc atggaccggg tgcgcggcgc
     3541 cattttttcc agtctcgggg aaaaggtggc cgatgccacc gtgctcgacc tttttgccgg
     3601 cagcggtggc ctgggcctcg aggcgctgag ccgcggcgcg gcgcggacga ccttcgtgga
     3661 cagcgactcg aagtgcggcc agatcatccg gcagaatctc acgcgctgcc acctcgccgc
     3721 cgcggtgcaa aacatggacg tcttccgctt cctcgagctt tacgccgagg aaaactcgtt
     3781 cgacctcatc ttcgccgacc cccctttcac caagaaaccc ggcgacttcg atttcgccac
     3841 cgcgctattg accaatccgc acctcaagcg cgccctgcat cccgaaggca tcttcattct
     3901 cgaaaagctc tactccagcc cccgccccgc cgacctgggc ggctggcagg tgcggcgcga
     3961 aaaacgctac ggggaatgcg aggtcttgtt tctcgcgcgg cccgaactct gaaaacatgg
     4021 gtttattttg gattttagat tttggatttt ggattgatga ggcatccgct tcatcccgtt
     4081 ccgttcgcgt ctcgtcccaa tcgcaaatcc aaaatccaaa atccaaaatc ggatgattct
     4141 tttcctctac aacctcttct tccccctcgt cctgctgttc atgctgccgg gctttctgct
     4201 gcgcatggtc cggcgcggca actaccggca caaattcggc cagcggttcg ccctttattc
//
LOCUS       DATDAY010000156         2524 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_1002915, whole genome shotgun sequence.
ACCESSION   DATDAY010000156 DATDAY010000000
VERSION     DATDAY010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2524)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2524)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2524
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1002915"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..481)
                     /locus_tag="VIS74_03040"
     CDS             complement(<1..481)
                     /locus_tag="VIS74_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HEY5792245.1"
                     /translation="MPTYIEMPKLSDTMTEGTLAKWRKKEGDKVEIGDILAEVETDKA
                     TMEMEAFDEGVLHKILIQDGGKAPVGGPLAVLLGEGEDASSISDKPAAPAEGKKAAAP
                     SAPATEKKEAVPVPAVTAPDNSRVKASPLAKKIAKERGVGLAGVTGTGPGGRVVAKDV
                     "
     gene            complement(541..1515)
                     /locus_tag="VIS74_03045"
     CDS             complement(541..1515)
                     /locus_tag="VIS74_03045"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF006667.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEY5792246.1"
                     /translation="MPVISYRKALNDAFAEEIERDENVLLIGEEVAQYNGAYKVTEGL
                     WAKYGDKRLVDAPISEAGFVGLGIGASMLGLRPVMEFMFWSFAYVAWDQIINNAGQVR
                     YMSGGLINCPIVLRGPANGGTNVGATHSHTPENFIANTPGLKVVCPATAYDAKGLMKS
                     AIRDNDPVCFMENTLLYGETWEVPEEEYLVPIGKADVKREGTDLSLIAHGRAVITALK
                     AAQKLQEEHNLNVEVLDLRSIRPLDEEAILETVRKTHRVVLVDENKPFCGVSAQIASI
                     IQEKAFDDLDAPILRVCSLDAPAIYSPAVEPLQLPTVERVIETVLKIS"
     gene            complement(1619..>2524)
                     /locus_tag="VIS74_03050"
     CDS             complement(1619..>2524)
                     /locus_tag="VIS74_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEY5792247.1"
                     /translation="LYIGQESVAVGQISLLGDHDHVITAYRDHGHALAVGMGMNECMA
                     ELFGKATGCSKGKGGSMHYFAPDKNYWGGHGIVAGQTPLGLGIAYALKYKGLKGSCLT
                     YLGDGAVNQGVFHESLNLAGLWNIPVIYVIENNKYSMGTSLERSSAIRNCLAERADGY
                     GIVWDIANGEDIYEVRAKTWNAITRAHEESRPTVLEVDTYRYYGHSVADANAKKYRSP
                     EEIERYKSHHDPINLWRKRLIEEEVLDEAGADAIDKAAKQEADASVQFAVESDDPTLE
                     SLFEDVYWEVDNHTERGQTGRHFFN"
BASE COUNT          452 a          753 c          772 g          547 t
ORIGIN      
        1 gaacgtcctt cgcgaccacg cggccgccgg ggccggtgcc ggtcacgcca gcgagaccga
       61 ctccgcgttc ctttgcgatt ttcttcgcga gcggcgaggc cttgacgcgg gagttgtcgg
      121 gcgcggtgac ggcgggaacc ggaacggctt ccttcttctc cgtggcggga gcggacgggg
      181 ccgccgcttt tttaccctcg gcgggcgccg cgggtttgtc gctgatgctg gaggcgtcct
      241 cgccttcgcc gagcaggacg gccagcggcc cgccgaccgg ggccttgccg ccgtcttgaa
      301 tcaaaatctt gtggagcacg ccctcgtcga aggcctccat ttccatcgtc gccttgtcgg
      361 tttcgacctc cgcgaggatg tcgccaattt ccaccttgtc gccctccttc ttgcgccact
      421 tggcgagggt tccctcggtc atcgtgtcac tgagcttggg catttcaata taagtcggca
      481 tagatgggaa aattaaaaat gaagaaaggc gaaaggcgaa gcccgaattg cggcggcttc
      541 tcagctgatt ttcagaacgg tttcgatgac gcgctccacg gtgggaagct gcaggggttc
      601 cacggccggc gaataaatgg ccggagcgtc cagtgagcag acccgcagga tcggggcatc
      661 gagatcgtcg aaggcctttt cctggatgat cgaggcgatt tgcgcggaca cgccgcagaa
      721 cggcttgttc tcgtccacca gcaccacgcg gtgcgtcttg cgaacggttt cgagaatggc
      781 ctcctcgtcc agcgggcgga tggaacgcag atcgagcact tcgacattga ggttgtgctc
      841 ctcctggagt ttctgcgcgg ccttgagcgc ggtgatgacc gcccggccgt gggcgatgag
      901 ggaaagatcc gtgccctcgc gcttgacgtc ggctttgccg atgggaacga ggtattcctc
      961 ctcgggcact tcccaggttt ccccgtagag aagggtattt tccatgaagc agaccgggtc
     1021 gttgtcgcgg atggccgact tcatgagccc cttggcatcg taggcggtcg ccggacagac
     1081 gaccttgagg ccgggggtgt tcgcgatgaa attctccggc gtgtgcgagt gggtcgcccc
     1141 cacgttggtg ccgccgttgg ccgggccgcg aagcacgatc gggcaattga tcaagccgcc
     1201 gctcatgtag cgcacctggc cggcgttgtt gatgatctga tcccacgcca cgtaggcaaa
     1261 gctccagaac atgaattcca tgaccgggcg caggccaagc atcgaggcgc cgatgcccag
     1321 gcccacgaaa ccggcttcgc tgatgggcgc atccacaagg cgtttgtccc cgtatttggc
     1381 ccaaaggcct tcggtcacct tgtaggcccc attgtattgg gccacttcct cgccgatgag
     1441 aagcacgttt tcgtcgcgct cgatttcttc ggcgaacgca tcgttcaggg ctttgcggta
     1501 tgaaatgacg ggcataaact aaaaactaaa cggaattgac gggattttca ggatttacgg
     1561 gaagttggcg gggcattttt cgaaccccgc atggcttccg atcttccaag gtgcgggctt
     1621 aattgaagaa atggcgtccg gtctggccgc gttcggtgtg gttgtccacc tcccaataca
     1681 cgtcttcaaa aaggctttcc aaggtcggat catcactctc gaccgcaaac tgcacggagg
     1741 cgtccgcctc ctgcttggcc gccttgtcaa tggcatccgc gccggcttca tccagcactt
     1801 cctcttcgat caggcgcttg cgccagagat tgatcggatc atgatgcgac ttatagcgtt
     1861 cgatttcctc ggggctgcgg tattttttcg cgttcgcatc cgcgacggaa tgaccgtaat
     1921 agcggtaggt gtccacttcg agcaccgtag gacggctttc ctcgtgcgcc cgcgtgatgg
     1981 cgttccaggt cttggcgcgg acttcgtaga tgtcctcgcc gttggcgatg tcccagacaa
     2041 tgccgtagcc atccgcgcgt tccgccaggc aattgcggat cgcgctggaa cgctccaggc
     2101 tggtgcccat cgaatacttg ttgttttcga tgacgtagat gaccggaatg ttccaaagcc
     2161 ccgccagatt cagggattcg tgaaaaaccc cctggttcac ggcgccgtcg cccagataag
     2221 tgaggcagga tcccttgagt cccttgtatt tcaaggcata agcgatgccc aggccgagcg
     2281 gggtctggcc ggcgacaatg ccgtggccgc cccagtaatt tttatccggc gcaaaataat
     2341 gcatcgatcc gcccttgccc ttcgagcagc cggtagcctt gccaaagagt tcggccatgc
     2401 actcgttcat gcccatgccc accgccaggg cgtggccgtg atcgcgatag gcggtaatca
     2461 cgtgatcgtg atcgccgagg agggaaatct gccccaccgc caccgattcc tggccgatgt
     2521 aaag
//
LOCUS       DATDAY010000157         4877 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_390332, whole genome shotgun sequence.
ACCESSION   DATDAY010000157 DATDAY010000000
VERSION     DATDAY010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 4877)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4877)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4877
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_390332"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            100..843
                     /locus_tag="VIS74_03055"
     CDS             100..843
                     /locus_tag="VIS74_03055"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEY5792248.1"
                     /translation="MKFRAISVWAASAGVFWLSGCDQVESWDNWMPGKPKIENVWKAP
                     TEITDFKELYAESCQGCHGMGNVVGAAIAMDNPTYLSLVTLEQLTEIISNGVKGTAMP
                     AFAIRNGGLLTDEQIHILAENILAGKNGQPAPAGPLPPYAAPLGSPAAGEKVYAAYQA
                     EAEKRSPGLTKDGFLTNPAFLDLVTDQYLRTLVIAGQPELGLLNWREAIPGQPLSDQE
                     IADLVAWLASKRVNEFGQPVPLTSAPSQP"
     gene            851..1426
                     /locus_tag="VIS74_03060"
     CDS             851..1426
                     /locus_tag="VIS74_03060"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEY5792249.1"
                     /translation="MNPAANPEEPKPCACEGHGAPEPEKKSRRDFLFHIGMGLNVLAG
                     ALITVPILGYVLGVVLKKTPLKPVSLGKVTNFPEGATRLASFQNPYHREEDGPTAHIP
                     CWVRRIQGNDFQVFAINCTHLGCPVRWFEEAKLFMCPCHGGAFYQDGRHASGPPPRGL
                     YTYEYQVLNGELFIMTGILPTLASEGHHDKV"
     gene            1423..2946
                     /locus_tag="VIS74_03065"
     CDS             1423..2946
                     /locus_tag="VIS74_03065"
                     /inference="COORDINATES: protein motif:HMM:NF012263.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HEY5792250.1"
                     /translation="MIELGKKVYAWFDDRLKLTRLHEHTSGHPIPGNSASWFYVFGSA
                     TLLCFVIQVLTGTLLAFSYVPSAAEAYSTLQFLTFEAPLGWYLRAIHYWGSNIMCAMM
                     LIHMTQVYLFGAYKYPRELTWVTGVLLLLCTLGAAFTGQVMRFDEDAYWGLGIGAAIA
                     GRTPVIGEQVVTLMLGGPILASETLGRFFSVHVFVIPGAIIALVSVHLRLVLLKGINE
                     YPKPGRVVRRETYDREYDEVIKKTGIPFFPNGIGKDLIFSAFTIILILFCAAVFGPKG
                     PLGPPDPVEIDTLPRPDFYFLWIFAVAALMPVWLEDFGLLVAPVLGILFLIALPFFNG
                     TGEKSWRRRPVAVLSVIFIWLSMALFTWLGYTSPWSPAMNAWTGDPIPEQFLKGRTPL
                     ELQGALVLQNKQCRNCHAIDGIGGQRGPDLADVGARLTKDQLIRQVIQGGGNMPAYGK
                     NLSPQEVAALAAYMVTLRPETINPARDSTFPAKPPEVSKEKKKEHAAVHAGSLADPS"
     gene            2953..>4877
                     /locus_tag="VIS74_03070"
     CDS             2953..>4877
                     /locus_tag="VIS74_03070"
                     /inference="COORDINATES: protein motif:HMM:NF021214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HEY5792251.1"
                     /translation="MNSAAEAAFLSWNFEPLQLGFLALAAGLYGRGWIKLNRQVPHRF
                     PVWRLGCYLGGILTLFLALASPLDAFASLLLLAHMTQHLMLTMVAAPLLLLGAPMLPM
                     LCGLPRIVAREVVGPFLSWPFLRKVGNLLTHPVFAWLAFVISNVVWHAPPLYELALRS
                     PAWHKVEHLCFLGTALLFWWFVIQPWPSRPRWPRWAVVPYLLLADLQNTALSAFLSFY
                     ESVLYPTYAAAPRLGRLTALEDQAGAGALMWVPGSMIFLIPVTRIAVDFLSRKRRMSA
                     LPPRVRPPQPPRERLDLLRLPVIRGKYFRQTLQIALLVLAALVVLDGLRGPQTSAMNL
                     AGVLPWTHWRGFTVVALLMVGNLFCMACPFTFVRDLGRRFLPARLAWPRALRSKWIAV
                     ALIVLYLWSYEIFDLWDNPAATAWIILGYFLAAFLIDGFFRKASFCKYVCPIGQFHFV
                     QSLVSPFEIKVREPEACAACRTHDCIKGNEQQRGCELQLFQPRKSGNMDCTFCLDCVK
                     ACPKDNVGMLPVWPGGDLLHDSFRSSVGKYSRRPDLAALMLVLVFGAFANAAGMLVPV
                     LRLAEKYPLPVTGVVLFLALTVAPLGAAIICGWLSRLLGGIRTKLSEVIGNQVPALVP
                     LGFSMWLAHFLFHLFTAS"
BASE COUNT          751 a         1578 c         1520 g         1028 t
ORIGIN      
        1 cggctacgtg gtttaccaca ccggcaaggt ccgcacgctg caaagcctga cggtctccgc
       61 ccggctgggc gtcgaggcgc aacaccgggg agagcattca tgaagtttcg ggcaatttca
      121 gtttgggcgg ccagcgcggg cgttttctgg ctttcgggtt gcgatcaggt ggagagctgg
      181 gacaattgga tgcccggcaa gccgaagatc gaaaacgtct ggaaggctcc cacggagatt
      241 acggatttca aggagctcta cgcggaaagc tgccagggct gccacggaat gggcaatgtc
      301 gtgggcgccg ccatcgcgat ggacaacccc acctaccttt ccctcgtgac gctggagcag
      361 ttgacggaaa tcatttccaa tggcgtgaag ggcaccgcca tgcccgcctt cgcgatccgg
      421 aacggcggcc tgcttaccga tgagcagatt cacatcctcg cggagaacat cctcgcgggc
      481 aagaacgggc agccggctcc cgcgggtccg cttccgccct acgccgctcc gctgggcagc
      541 ccggcggcgg gtgagaaggt ctatgccgcc taccaggccg aagccgaaaa acggagcccc
      601 ggccttacca aggatggctt tctgaccaat cccgccttcc tcgatctggt gacggatcaa
      661 tacctccgca cgctggtgat cgccggccag ccggaactgg gcctgctgaa ctggcgcgaa
      721 gccatccccg gccagccctt gagcgaccag gagatcgccg acctggtggc ctggctcgct
      781 tcgaaacggg tcaatgaatt cggccagccc gtgccgctga catccgcacc cagccaacct
      841 tgagtttttt atgaatccag cagccaatcc cgaggaaccc aaaccctgcg cctgcgaggg
      901 tcacggcgct cccgagccgg agaaaaagtc gcgccgcgat tttctctttc atatcggcat
      961 gggtttgaac gtgctggccg gcgcgttgat caccgtgccg attctcggtt acgtccttgg
     1021 cgtggttctc aaaaagaccc cgcttaagcc ggtttccttg ggcaaggtca cgaattttcc
     1081 cgaaggcgcc acccggctgg ccagctttca gaacccctac caccgcgaag aggacggccc
     1141 gaccgcgcac atcccctgct gggtgcgccg gattcagggc aatgacttcc aggtttttgc
     1201 catcaattgc acgcacctcg gctgcccggt ccgctggttc gaggaagcca agctgttcat
     1261 gtgcccctgc cacggcggcg cgttttacca ggacggacgc cacgcctccg gcccgccgcc
     1321 gcgcggactc tacacttacg aatatcaggt gctcaacggc gaacttttca tcatgacggg
     1381 aattctgcct acgcttgcca gcgagggcca ccacgacaaa gtatgatcga gctcggcaaa
     1441 aaagtttacg cgtggtttga cgaccggctg aagctcaccc ggctgcacga gcacacctcc
     1501 gggcacccca ttcccggcaa tagcgcaagc tggttttacg tcttcggcag cgccacgctg
     1561 ctctgcttcg tcattcaggt tctgacgggc acgctcctgg ccttttccta cgtgccctcg
     1621 gcggcggagg cgtattcgac gctgcaattc ctgaccttcg aggccccgct gggctggtat
     1681 ctgcgcgcga tccattattg gggatcgaac atcatgtgcg ccatgatgct cattcacatg
     1741 acgcaggtgt atcttttcgg cgcttataaa tacccgcgcg aactcacctg ggtgaccggc
     1801 gtgctcctgc tgctctgcac gctgggtgcc gcctttaccg ggcaggtcat gcggttcgat
     1861 gaagacgcct actggggcct cggcatcggc gcggccatcg ccggccgcac gccggtcatc
     1921 ggcgaacagg tcgtcacgct catgctcggc ggcccgatcc tcgcctccga aacgctgggc
     1981 cgctttttct cggtgcatgt gtttgtcatc ccgggcgcca tcattgcgct ggtgagcgtc
     2041 cacctccggc tggtgctcct caagggcatc aacgaatacc cgaaaccggg ccgcgtggtg
     2101 cgccgcgaga cgtatgaccg cgaatacgac gaggtcatca aaaaaaccgg cattcccttt
     2161 ttcccgaacg gcattggcaa ggatctgatt ttctccgcgt tcacgatcat tttgatcctg
     2221 ttctgcgcgg cggtgtttgg cccgaagggg ccgctgggac cgcccgatcc ggtggaaatc
     2281 gacacgctgc cgcgcccgga cttttatttc ctgtggatct tcgcggtcgc ggcgctcatg
     2341 cccgtgtggc tggaagactt cggcctgctg gtggcgccgg tgcttggcat cctgttcctg
     2401 atcgctctgc ccttctttaa tggcaccggc gaaaagagct ggcgccgccg cccggtggcc
     2461 gtgctttcgg tcattttcat ctggctctcg atggccctgt tcacctggct gggttacacc
     2521 tcgccgtggt cgccggcgat gaatgcctgg acgggcgatc ccattcccga gcaatttctc
     2581 aagggccgca ccccgctgga actgcagggc gcgctcgtgc tgcaaaacaa gcagtgccgg
     2641 aactgccatg ccatcgatgg cattggcggc cagcgcggtc cggatttggc cgacgtgggc
     2701 gcccgcctga ccaaggatca attgatccgc caggtcattc agggcggcgg caacatgccg
     2761 gcttacggaa agaatctctc gccgcaggaa gtggcggcat tggcggctta catggtgacg
     2821 ctgcggccgg agacgatcaa tccggcccgc gattccacct tcccggccaa accgcccgag
     2881 gtctccaaag agaagaagaa ggaacacgcc gcggttcacg ccggatccct ggcggatcct
     2941 tcctgatccg cggtgaattc ggcggcggaa gctgcgttcc tgtcctggaa ctttgagccg
     3001 ctccagctgg gcttcctggc gctggcggcc ggcctgtatg ggcgcggctg gatcaagctg
     3061 aaccggcagg tgccgcaccg gtttccggtc tggcggctgg ggtgttacct cggcggcatt
     3121 ctgacgctgt tcctcgcgct ggcttcgccg ctggatgcct tcgcctccct gctgctgctg
     3181 gcccacatga cccagcatct gatgctgacc atggtggcgg cgccgttgct gctgctgggc
     3241 gcgccgatgc tgccgatgct ctgcggattg ccgcgcatcg tcgcccgcga ggtggtgggc
     3301 cccttcctgt cctggccgtt tttgcggaag gtgggaaatc tcctgacgca tccggtcttc
     3361 gcctggctgg cgtttgtgat ctcgaacgtg gtctggcacg cgccgccgtt atacgaattg
     3421 gccctgcgct cgcccgcctg gcacaaggtg gagcatcttt gcttcctcgg cacggcgctt
     3481 ttgttctggt ggtttgtcat ccagccctgg cccagccgcc cccgctggcc ccgctgggcg
     3541 gtggtcccct atctgctgct ggcggatttg cagaacaccg cgctttccgc ttttctgtcc
     3601 ttctacgagt cggtgcttta tccgacctac gccgccgcgc cgcggctggg caggctcacc
     3661 gcgctcgagg atcaggccgg cgccggggcg ttgatgtggg tgccgggctc gatgattttt
     3721 ctcattccgg tgacgcgcat cgccgtggat ttcctttccc gcaagcgccg gatgtccgcg
     3781 ctcccgccgc gggtgcggcc gccgcagccg ccgcgcgagc gcctggattt gctgcgcctg
     3841 ccggtgattc ggggaaaata tttccggcaa acgctgcaga ttgcgctgct ggttctggcg
     3901 gcgctggtgg tgctggacgg cctgcgcgga ccccagacca gcgcgatgaa cctcgccggc
     3961 gtgctgccct ggacccactg gcgcggattc accgtcgtgg cgctgctgat ggtggggaat
     4021 ctcttctgca tggcctgccc gttcacgttc gtccgggatc tggggcggcg gttcctgccc
     4081 gcccgtctgg cctggccgcg ggcgctgcgc tccaagtgga tcgcggtggc gctcatcgtg
     4141 ctttacctgt ggtcctacga aattttcgac ctctgggaca atccggcggc gacggcgtgg
     4201 atcatcctgg gctatttcct ggcggcgttt ctcatcgacg gctttttccg caaggccagt
     4261 ttctgcaaat acgtctgccc gatcggccag ttccattttg tgcagtcgct ggtctcgccg
     4321 ttcgagatca aggtgcgcga gccggaggcg tgcgcggcct gccggacgca tgactgcatc
     4381 aagggcaacg aacaacagcg cggctgcgag ctgcagcttt tccagccgcg caaatcgggg
     4441 aacatggact gcacgttttg cctcgattgc gtgaaggcct gcccgaagga caatgtggga
     4501 atgctgcccg tctggcccgg cggggatttg ctgcacgatt ccttccgctc ctcggtaggc
     4561 aaatactccc gccgcccgga tttggcggca ttgatgctcg tcctcgtctt cggcgccttt
     4621 gccaatgcgg cgggcatgct cgtgccggtg ctccggctgg cggaaaaata tccgctgccg
     4681 gtgaccggcg tggttttgtt cctggcgctg acggtggcgc cgctcggcgc ggcgatcatc
     4741 tgcggatggc ttagccggct gctgggcggc atccgcacga aactttccga ggtgatcggc
     4801 aatcaggttc cggcgctcgt gccgttggga ttttccatgt ggctcgcgca ttttttgttt
     4861 cacctcttta cggcctc
//
LOCUS       DATDAY010000158         6924 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_1321007, whole genome shotgun sequence.
ACCESSION   DATDAY010000158 DATDAY010000000
VERSION     DATDAY010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 6924)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6924)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6924
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1321007"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(122..319)
                     /locus_tag="VIS74_03075"
     CDS             complement(122..319)
                     /locus_tag="VIS74_03075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792252.1"
                     /translation="MGLAFGPPALRQWRILSQRGSKALDIVAEIEAEQALVAANRELI
                     TRFERKIQATLARIWGETETA"
     gene            complement(390..938)
                     /gene="tnpA"
                     /locus_tag="VIS74_03080"
     CDS             complement(390..938)
                     /gene="tnpA"
                     /locus_tag="VIS74_03080"
                     /inference="COORDINATES: protein motif:HMM:NF013921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HEY5792253.1"
                     /translation="MRKRTSKDQSPESGARIPTAPTAHRHPSLGQRPRNHLSFLLVHV
                     VFSTKERAPILDASVRPALHAYLATVARNADCECYRVGGIEDHVHLAIRLSRTITTAK
                     LIEELKTSSSKWLKTQSPALAAFAWQRGYGAFSVGPPDLDALRHYIDTQEEHHRTRTF
                     QEEYRAFLTKYGIEFDERYVWD"
     gene            complement(1511..1687)
                     /locus_tag="VIS74_03085"
     CDS             complement(1511..1687)
                     /locus_tag="VIS74_03085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792254.1"
                     /translation="MNDILDLDLPEIAPGEGPLPLAEPSYELERDHARFLLRSRGARE
                     DLNQTPNPEPFVLD"
     gene            complement(1671..2225)
                     /locus_tag="VIS74_03090"
     CDS             complement(1671..2225)
                     /locus_tag="VIS74_03090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792255.1"
                     /translation="MPEDRDIFQICVDEAAAAGLDFLLIGGHAINARGYTRTTLDFDF
                     LIAGRDLRAWSKIFESIGYRLITENIKAFAQFEPREGEGFRVDLMLVDDSTFAKLLAG
                     SEWRDYGRRRVRVAGVLHLIALKLHALRSEERASLGKDYLDILNLIRLNRVDIHSVEF
                     EEVVNRYGSPSIRDRIRQDRERYP"
     gene            complement(2378..2968)
                     /gene="rdgB"
                     /locus_tag="VIS74_03095"
     CDS             complement(2378..2968)
                     /gene="rdgB"
                     /locus_tag="VIS74_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HEY5792256.1"
                     /translation="MPLPLLLSTRNAHKLQEVREILGNAFEVTGLAAIPNFPEVEETG
                     ETFEENAALKAVAASLHFDGWVIADDSGLEVDALGGAPGVYSARYAGGKSNDAANNAL
                     LLKNLETARGKERSARFRCVIVVARAGRKLAAISGAGEGVIINQPKGESGFGYDPLFV
                     PEGYCETFAQLGAAVKNTLSHRARALEKLRDWRGWF"
     gene            3029..6304
                     /locus_tag="VIS74_03100"
     CDS             3029..6304
                     /locus_tag="VIS74_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEY5792257.1"
                     /translation="MSSQRKQFPFPEFEPAWQKRWLETGAFRAANPGDADFDAAKPKY
                     YVLDMFPYPSGEGLHVGHVEGYTATDIIARFKRMQGFNVLHPMGWDAFGLPAEQFAIK
                     TGQHPRVTTERNIARFKTQLRGIGFSYDWDREISTTDPGYFKWTQWIFRKLYHSWFNP
                     VTQKAEPIETYAGNDPDGVRLAFVSEVPVNWCPELGTVLANEEVVEGKSEVGGFPVER
                     RPLRQWMLRITAYAERLIDELDGLDWPESIKLLQRNWIGKSQGAMVEFSICGEMGPPH
                     PHQDFEPSEARQTAEGSPKGELASQFSIEVFTTRPDTLFGATYMVLAPEHMLVPKITT
                     VEQRAAVEVYQKQVGSKSDLERTDLAKDKTGVFTGAYAINPVNGERIPIWIADYVLMG
                     YGTGAIMAVPAHDERDFEFARKFGLEIRPVVQPASGIGFQPMRSFDKVEIREGANLPH
                     WTQEKATYSATFRLADSLPESALAELSEDRRYLIAKAGRGEPLSSAEAAEWRKLFTEK
                     IESLLDAGHGDCPLGKPEIATLVENALKHFDGERYDLLAWCVMPNHVHVVFTPIGDHS
                     LSGILHSWKSFTANKANEALGRTGEFWQRESYDHLIRNSEEFSRQVQYALANPEKAGL
                     RDWKWAGRKETHRQDADATEVMDCYAGEGTAVNSQFLDGLPTAEAKQRITAWLEEKGI
                     GRGTVNYKLRDWLFSRQRYWGEPFPIVWENGRHRSVDETELPVLPPEMDDFKPTGTPE
                     PPLSKVKEWLHYSEAATRETNTMPQWAGSCWYYLRFCDAENPNRFVGEEAERYWMGAN
                     PKSQGVDLYVGGTEHAVLHLLYARFWHKALFDLGCVSTREPFHKLVNQGIILGPDNQK
                     MSKSRGNVVNPEQVIVEFGADSLRLYEMFMGPLEQMKPWSDKGVEGVYRFLARVWRLA
                     MEETQEGGWVLAPELAELELTPAQLRVAHATIKKVTEDIQAFAFNTAIAQMMVFTNEF
                     VNANPRPRAALRILLPLLSPFAPHLAEELWERLEFEGRASAQPWPVHDPRLLIVDEVE
                     YVVQVNGKVRDRLTVKKDAADAEIETLARASEKVKEFTAGGAVVKVIIVRGKLVNLVV
                     RSN"
     gene            6464..>6924
                     /locus_tag="VIS74_03105"
     CDS             6464..>6924
                     /locus_tag="VIS74_03105"
                     /inference="COORDINATES: protein motif:HMM:NF016830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HEY5792258.1"
                     /translation="MKRLLLPYAVVLSFLAAAPLPAESGHLNINGETNGHPRFWDYYT
                     PCPSNYIGSSLWWQGETLTGDWWGTRNWLKDSGVEVVFNYTTNLAGNPVGGQSQGFTY
                     TDNIYFGLQLDLEKLAGWNGAQFTISGLDRNGTSLSQNFLGNQFTVQQIYGG"
BASE COUNT         1374 a         2042 c         2176 g         1332 t
ORIGIN      
        1 tggcgcccgg cgaaattggg aggggcaaag ccccgcatcc atcccagcct aggccatcgg
       61 cctaggtttt ggttcgcgca aaacgaccag ggctgacagc ccgattcatc gtgcggcgcg
      121 ctcatgcggt ttccgtttcc ccccagatgc gggcgagggt ggcttggatc tttcgctcga
      181 aacgggtgat cagttcgcgg ttggcggcca ccagcgcctg ctcggcttcg atctcggcca
      241 cgatgtcgag ggccttcgac cctcgctggg ataagatgcg ccattggcgc agggctggtg
      301 ggccaaaggc caatcccatc ccagcctggg gcaacgcccc aggaaacgtg accaagaaaa
      361 catcaagggc tgaaagcccg ttccatcgct caatcccaca catagcgctc atcaaattcg
      421 attccgtatt tcgtgagaaa cgcacggtat tcctcctgaa atgtccgcgt tcggtgatgc
      481 tcctcctgcg tgtcgatgta atgcctcaac gcatccaaat ccggcggccc gacggaaaat
      541 gccccgtagc cacgttgcca tgcaaaggct gccaatgcgg gcgattgagt tttcagccat
      601 ttcgaggatg aggttttcaa ctcctctatc agtttcgccg tggtgatggt gcgagacagc
      661 cggattgcaa gatgcacatg atcctcgatg cctccgacac ggtaacattc gcaatcagcg
      721 ttcctcgcga ccgtggcgag ataggcatgg agcgcgggac ggacggaggc atcgaggata
      781 ggcgcgcgtt ctttggtgct gaagacaacg tggacgagga gaaatgaaag atggttcctg
      841 gggcgttgcc ccaggctggg atggcgatgc gccgttggcg cggtcggaat cctcgcccca
      901 gactcgggcg attgatcttt ggatgttctt ttcctgatcc gcgatctcgg ctttgaggcg
      961 tttgatctcg ttctggtagc cctcgatctc cgccacgatc tccttctgca cctccagcga
     1021 cggcaggggg atgcgtgagt cgaagatccg aggcatattc caaacgaagc cggaagtgtt
     1081 tcagcccttc ccaatttttt ccagcgggaa cgccccgatt tgcatggcca agcccgcccc
     1141 cgaacatgaa cttccaaata ccacgcgatt cgggatggcg gaagccctcc ggctgatcac
     1201 ttcgcggctt ggcggctttg cgtgagacgg gtcttgctcc caggctttat ttcggcggca
     1261 agggcctgcc tccctccatt tgaatgagcg ggtgtggcat cggcatcctg ccgatgccac
     1321 ttctaaaacg agccgcgcca gtcgcggagt tttcccatgg acgcggtgaa tccttctgaa
     1381 acttggttgt tcgcacaacc tccggccttc ggcggtgaaa tggttttccc ctacagccgt
     1441 tcgccgtaaa gaaggggcca aggggcggga agggaagaag gtcgtcactg tcctgaagag
     1501 agtcccggaa tcaatcgagc acgaagggtt cgggattcgg cgtttggttc aagtcttcac
     1561 gcgcgccccg ggagcggaga agaaaccgcg cgtgatccct ttccagctca taggaaggct
     1621 ccgccaaggg cagcggcccc tcgcccggag cgatttccgg aaggtcgaga tcaaggatat
     1681 cgttcacggt cttgccggat gcggtctcga atgcttgggc ttccataacg attgacgact
     1741 tcttcgaatt ccaccgagtg aatgtcaacc cggttcaggc ggatgagatt gagaatgtcc
     1801 agataatcct tccccagcga agcgcgctcc tcgctgcgca gggcatgaag tttgagcgcg
     1861 atcagatgaa gcaccccggc gacccgcacg cgccttcggc cataatcccg ccattcgctg
     1921 ccggccagca gcttggcaaa tgtggaatca tccaccagca tcaaatcgac ccggaatccc
     1981 tccccctccc gcggctcgaa ctgggcaaag gccttgatat tctcggtgat gagccggtag
     2041 ccaatggact cgaaaatttt gctccacgcc cgcaaatcgc ggcccgcgat caggaaatcg
     2101 aaatccaagg tcgtgcgcgt ataaccccgg gcattgatcg catggccgcc gatcaggaga
     2161 aaatccaagc cggccgccgc cgcttcgtcc acgcagattt ggaaaatgtc gcgatcttca
     2221 ggcatccccg ccaaacatag cgacagaaga gggtggcgtc gatcatcgtt gagagtttgg
     2281 aaaccatcgg ccgcttccgc tcccttttca cttcggcggc aagggcctgc cgccctccaa
     2341 atcgaacgag cgcgagcact tccagccggt gcgacttcta aaaccagccg cgccagtcgc
     2401 ggagtttttc caatgcccgg gcgcggtggc tgagcgtatt tttcaccgcc gcgccgagct
     2461 gggcaaaggt ttcgcagtag ccttcgggca caaagagcgg atcatacccg aagccgcttt
     2521 cgcctttggg ctgattgatg atcactccct cgcccgcgcc gctgatggcg gcgagcttcc
     2581 ggccggcgcg ggcgacgacg atgacgcagc ggaatcgcgc ggagcgctcc ttgccgcgcg
     2641 cggtttcgag atttttcagg agcagcgcgt tgttcgcggc gtcgttggat tttcccccgg
     2701 cgtagcgggc ggaatagacg ccgggcgcgc cgccgagcgc gtccacctcg aggccggagt
     2761 cgtcggcgat gacccagccg tcgaaatgca gggacgccgc cacggctttg agcgcggcat
     2821 tttcctcgaa ggtttcgccg gtttcctcga cttcgggaaa gttgggaatg gccgccagac
     2881 cggtcacttc gaaggcattt ccgaggattt cgcggacctc ctggagcttg tgggcgttgc
     2941 gggtggaaag aagcagggga agcggcataa atttcgtgat tcctctttac gccggagcat
     3001 tgcagagtat ccagcccatt tccttttcat gtcgtcccaa cgcaaacagt ttccgtttcc
     3061 cgaatttgaa cccgcctggc aaaagcgctg gctggagact ggcgcgttcc gcgcggccaa
     3121 tcccggcgac gcggacttcg acgccgcgaa gccgaaatac tacgtgctcg acatgttccc
     3181 gtatcccagc ggcgaggggc tgcacgtggg ccacgtggag ggttacacgg ccacggacat
     3241 catcgcgcgc ttcaagcgca tgcagggctt caacgtgctg catcccatgg gctgggacgc
     3301 gttcgggctg cccgcggagc aatttgccat caagaccggc cagcatccgc gggtcacgac
     3361 ggagcggaac atcgcgcgct tcaagacgca gttgcggggc atcgggttca gctacgattg
     3421 ggaccgcgag atcagcacga cggacccggg atattttaaa tggacgcagt ggatttttcg
     3481 gaagctttac cattcgtggt tcaatccggt gacgcaaaaa gcggagccga tcgaaaccta
     3541 cgccgggaat gatcccgacg gcgtgcgcct ggcgttcgtc tcggaggtgc cggtgaactg
     3601 gtgcccggaa ctcggcacgg tgctggcgaa tgaggaggtc gtggagggca aaagcgaggt
     3661 gggcggattt ccggtcgagc gccggccgct gcgccaatgg atgcttcgga tcacggctta
     3721 cgcggaacgc ttgattgatg agctggacgg gctcgattgg ccggagtcga tcaagctgct
     3781 tcagcggaac tggatcggga agtcgcaggg agccatggtg gaattttcga tttgcgggga
     3841 gatggggccc ccgcatcccc atcaggattt tgagccgagc gaagcgagac agactgccga
     3901 aggcagccca aagggcgagc ttgcgagtca attttcgatt gaggttttca ccacgcggcc
     3961 ggatacgctg tttggggcga cttacatggt gctggcgccg gaacatatgc tggtgccgaa
     4021 gatcacgact gtggagcagc gcgcggcggt ggaggtttac cagaagcagg tggggtcgaa
     4081 gtcggatctg gagcgcacgg atctggcgaa ggacaagacg ggcgtgttca ccggggctta
     4141 tgccatcaat cccgtcaacg gcgagcgcat tcccatctgg atcgcggatt atgtgctgat
     4201 gggctacggg acgggcgcga tcatggccgt gccggcgcac gacgagcgcg acttcgaatt
     4261 cgcgcggaaa tttgggttgg aaatccggcc ggtcgtgcag cccgcaagtg gcatcggctt
     4321 ccagccgatg cgttccttcg acaaagtcga aatccgggaa ggcgccaacc tgccgcattg
     4381 gacgcaggag aaggccactt actccgccac cttccggctg gccgattccc tccccgagtc
     4441 cgcgctggcg gagctctccg aagaccggcg ttatctcatt gccaaagccg gacgaggcga
     4501 gccgctgagc tccgccgaag ccgcggaatg gagaaaactc ttcacggaaa aaatcgagag
     4561 cctgctcgac gcgggtcacg gtgattgccc actcggcaaa ccggagatcg caaccctcgt
     4621 ggaaaatgcc ctcaagcact ttgatggcga acgctatgac ttgctggctt ggtgcgtgat
     4681 gccgaaccat gtccacgtcg tttttacccc gatcggggac cattcacttt ccggcattct
     4741 gcactcgtgg aaatcgttca cggcaaataa ggccaatgag gcgctcggac gaacgggtga
     4801 attctggcag agggagtctt acgatcatct catccggaac tcggaagagt tttcccggca
     4861 ggttcaatac gcgctggcaa atccggagaa ggcggggctg cgggattgga agtgggcggg
     4921 ccggaaagaa acgcatcggc aggatgccga tgccacggag gtgatggatt gttacgcggg
     4981 cgagggcacg gcggtgaact cgcagttcct cgacggcctg ccgacggcgg aggcgaagca
     5041 gcggatcacg gcgtggctgg aggagaaggg catcggccgc ggcacggtca attacaagct
     5101 gcgcgactgg ctgttctcgc gccagcggta ttggggcgag ccgttcccga ttgtctggga
     5161 aaatggccgg catcgcagcg tggacgaaac cgagttgccc gtgctgccgc cggaaatgga
     5221 cgacttcaag ccgaccggca cgcccgagcc gccgctctcg aaggtgaagg aatggctcca
     5281 ttattccgaa gccgccacgc gcgaaaccaa tacgatgccg caatgggccg gttcgtgctg
     5341 gtattacctg cgcttctgcg acgccgaaaa tcccaaccgc ttcgtcggcg aggaagccga
     5401 acgctactgg atgggcgcca atccaaaatc ccagggcgtc gatctctacg tcggcggcac
     5461 ggagcacgcc gtgctgcact tgctctacgc gcggttctgg cacaaggcgc tgttcgatct
     5521 cggctgcgtc agcacgcgcg agccgttcca caagctcgtc aaccagggca tcattctcgg
     5581 cccggacaac caaaagatgt ccaagagccg cggcaatgtc gtgaatcccg agcaggtgat
     5641 cgtggaattc ggcgcggact cgctgcggct ttacgagatg ttcatggggc cgctggagca
     5701 gatgaaaccc tggagcgaca aaggcgtcga gggcgtgtat cgcttcctcg cccgcgtgtg
     5761 gcggctggcc atggaggaaa cgcaggaggg cggctgggtt cttgcgccgg agctggccga
     5821 gctcgagctc acgcctgcgc agttgcgcgt ggcgcatgcg accatcaaaa aggtcaccga
     5881 ggacatccag gccttcgcgt ttaatacggc gatcgcgcag atgatggtct tcaccaacga
     5941 attcgtgaat gcgaacccgc gcccgcgggc ggcgctgcgc attctgctgc cgctgctctc
     6001 gccgttcgcg ccacacctgg cggaggaatt gtgggagcgg cttgagttcg aaggacgcgc
     6061 ttccgcccag ccctggccgg tgcatgatcc gcggcttttg atcgtggacg aggtggaata
     6121 cgtcgtgcag gtcaacggca aggtccgcga ccggctcacc gtgaaaaaag acgcggcgga
     6181 cgcggagatc gaaaccctcg cgcgggcgtc ggagaaggtg aaggaattca ccgcgggcgg
     6241 cgccgtcgtg aaggtcatca tcgtgcgggg caagctggtg aatctcgtcg tgcggagcaa
     6301 ctaaacggga ttgcgggatt ttccggatta acgggagaaa attgcaaaac gggttttgca
     6361 aatccggtga atccgtgatc ccgtttagtt cctgtcgccg aggcgcaacc ggcattgcca
     6421 ccgcccgacg tttttgattg aatccgttcc catctcccca cttatgaagc gcctcctcct
     6481 cccgtatgcc gtcgttttgt cgttcctcgc cgccgcgccg ctgccggcgg aaagcggcca
     6541 tctcaacatc aacggcgaaa cgaacggcca tccccgattc tgggattatt acactccctg
     6601 tccgtccaac tacatcgggt ccagcctctg gtggcagggg gaaacgctca ccggcgactg
     6661 gtggggcacg cggaactggc tgaaggactc cggcgtggaa gtcgtcttca attacaccac
     6721 gaacctcgcg ggcaatcccg tcggcggcca aagccagggc tttacctaca cggacaacat
     6781 ttacttcggc ctgcagctcg atctcgaaaa attggcgggc tggaacggcg cgcagttcac
     6841 catcagcggc ctggaccgga atggcaccag cctttcgcag aactttctcg gcaatcagtt
     6901 caccgttcag cagatctacg gcgg
//
LOCUS       DATDAY010000159         3390 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_1808994, whole genome shotgun sequence.
ACCESSION   DATDAY010000159 DATDAY010000000
VERSION     DATDAY010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 3390)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3390)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3390
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1808994"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1298)
                     /locus_tag="VIS74_03110"
     CDS             complement(<1..1298)
                     /locus_tag="VIS74_03110"
                     /inference="COORDINATES: protein motif:HMM:NF024973.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HEY5792259.1"
                     /translation="MGRALFRVAGLGLLAVVSLAAAQAVEVVSSLSEDTVPVGRPVQF
                     QVQVQGASSADVPDNFAVDGLDIRFAGQSNQVQMRNFQVSMSVLYTFLVIPERAGNFT
                     IPGLSVVVGGKVYKTEAHRLQVTGSGSLPFAPPPQFQTRPQVQFPVAPPVPPPIPPPS
                     RTPSLDKLAYAELIIPESKAFVGQNIPVEIRFYFDSRYRIEVNSRPGFGDEGFTAEKL
                     SEPREKTQVIGGIPFNVFSFKTAITPIKPGPLKISPALLQAVITVPSDRAPANDFFGS
                     LFGTMGGEQREVRIESNPVDLEVKPLPKEGRPAGFAGAIGRFALDAEASPHETGAGEP
                     VTLKLRVKGQGNFNAIAEPELTGKDGWRIYPGATDFTPSDDTGFSGTKTFESMLVAVE
                     PKTQTPGAEFSYFDPSAEKYVTLAATPVPVKAQAGKAAPPA"
     gene            complement(1336..3306)
                     /locus_tag="VIS74_03115"
     CDS             complement(1336..3306)
                     /locus_tag="VIS74_03115"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEY5792260.1"
                     /translation="METTAVKFSFGEPVWLFALAVLPVIALLFFLSQRRSEKLIARIV
                     SPRLKQQLAGAVSVGLRFLRAVLILSALALGIVCLAKPRTGYIEQEIKQRGRDVILAF
                     DVSRSMLATDVLPARLARAKLFARDLIGYLGNDRVGVVAFAGSAFLQAPLTQDQTAVL
                     ATIDELDTDLIPRGGTNLAAAIDTALKAFGDAEGVTRALVIVTDGEELDADGLAAAHR
                     AAEKHIRILTVGVGSEEGSLIPLRTPEGKADFVRDSGGKPVMTRLDAARLREIAEIAG
                     GFYQALGPDAAAAVYEKGIQPLEEVEQKSSATRVPIENYQIPLGAAIGLILLWLLLGE
                     KKWPAFFARKRTKTAIPKAALAGAFLLGLPAAGWAQSADKYQSGDFAGALGDYERQLA
                     KHPNADKFSFNAGASAYRLGDFGKAVDHFTKALLAEDKTLQEDAAYNLGNALVRRGEK
                     AAETEAKKKDWENAIEYYNQALKLNSKDNRAKENIEVVKKLLEDLQKQDQQQKQDQQQ
                     KQDQQQKQDQQQKQDQQQKQDQQQKQDQQQKQDQQQKQDQQQKQDQQQKQDQEQPQQE
                     PEPPQNQAAPSKGDQPLPDLPEPGEKKQGELSAQNPSEEQKEAEAAEAAEGPDGKMSE
                     AQARALLRSLQNEEDRVRVMERKAQQETTRDW"
BASE COUNT          561 a         1108 c         1029 g          692 t
ORIGIN      
        1 gccggcggtg cggcttttcc ggcctgggct ttcaccggca cgggagtggc ggcaagcgtg
       61 acgtattttt ccgccgaggg atcgaagtag gaaaactccg cgccgggcgt ttgggtcttg
      121 ggctccacgg cgacgagcat gctctcgaac gtcttcgtgc cgctgaagcc ggtatcatcg
      181 gacggcgtaa aatccgtcgc cccggggtaa atgcgccagc cgtccttgcc ggtcagctcg
      241 ggttcggcga tggcgttaaa attgccctgt cccttgaccc gcagcttcag ggtgaccggc
      301 tcgcccgccc cggtttcgtg gggagaagct tcggcatcga gggcgaatcg gccgatggct
      361 ccggcaaatc ccgcggggcg gccctccttc gggagcggct tgacctcgag gtccaccgga
      421 ttgctttcga tccgcacctc acgctgctcg ccgcccatgg ttccgaagag gctgccaaaa
      481 aaatcattgg ccggcgcgcg gtcgctcggc acggtgatca ccgcctgaag cagggccggg
      541 gaaatcttga gcgggcccgg cttgatgggg gtgatcgccg ttttgaagct gaagacatta
      601 aacggaatgc cgccgatgac ctgcgttttt tcccgcggct cggaaagttt ttccgcggtg
      661 aatccctcgt cgccaaagcc ggggcgcgag ttgacttcga tgcggtaacg gctgtcaaaa
      721 taaaaccgga tttccaccgg gatgttttgc ccgacgaacg ccttgctctc gggaatgatc
      781 aattcggcat aggcgagttt gtccagcgac ggggtccgcg aaggcggcgg gatgggcggg
      841 ggaaccggcg gcgccaccgg aaattgaacc tgcggccggg tctggaactg gggcggcggc
      901 gcaaatggca ggctgcccga gccggtcacc tggaggcggt gcgcttcggt tttatagacc
      961 ttgccgccca cgaccacgct caagccgggg atggtgaaat tccccgcgcg ctccggaatg
     1021 accaggaagg tgtagagcac ggacatcgaa acctggaaat tgcgcatctg cacctggttc
     1081 gattgtccgg caaagcggat gtccaagccg tccacggcaa aattatcggg cacgtccgcc
     1141 gaagacgccc cctgcacctg gacctgaaat tgaaccggcc gccccacggg caccgtgtcc
     1201 tcggagagac tggagacgac ctcgaccgcc tgggcggcgg ccagggaaac gacggccagc
     1261 agccccaggc cggccacgcg gaaaagcgcg cgccccattc ctccaatgga ttttttctgt
     1321 atcgccataa cctgcctacc aatcgcgggt ggtttcctgc tgtgcctttc gttccatgac
     1381 tctaacccga tcctcctcat tttgcaggga tcttagcaag gctcgggcct gggcttcgct
     1441 cattttgcca tccggcccct cggcggcttc cgcggcctcg gcttcctttt gttcctcgga
     1501 ggggttctgg gcgctgagtt ccccctgctt tttctctccc ggctccggca aatcgggcaa
     1561 tggctggtcg cccttgctgg gcgcagcctg gttttgcggg ggttccggtt cctgttgcgg
     1621 ctgctcctga tcctgcttct gttgctgatc ctgcttctgt tgctggtcct gcttctgctg
     1681 ctggtcctgc ttctgttgct gatcctgctt ctgctgctgg tcctgcttct gctgctgatc
     1741 ctgcttctgc tgctgatcct gcttctgttg ctgatcctgc ttctgttgct gatcctgctt
     1801 ctgctgctgg tcctgcttct gcaaatcctc gagcagtttt ttgacgacct cgatgttttc
     1861 cttcgcccgg ttatccttgg aattcagctt gagggcttgg ttgtaatact cgatggcgtt
     1921 ctcccagtcc ttcttcttcg cttcggtctc cgccgccttc tcgccccggc gcacgagcgc
     1981 gttgcccaaa ttataagccg cgtcctcctg cagggttttg tcttccgcca gcagcgcctt
     2041 ggtaaaatga tccaccgcct tgccgaaatc tcccaggcga tacgccgagg cgccggcatt
     2101 gaagctgaat ttgtccgcgt tggggtgctt cgcgagctgc cgctcgtaat cccccaacgc
     2161 gcccgcaaaa tcgccggact gatatttgtc cgcggactgc gcccagcccg cggccgggag
     2221 tcccagcaga aacgccccgg ccagcgcggc tttcgggatc gccgttttgg tccgtttccg
     2281 ggcgaagaag gccggccatt ttttctcgcc gagcaacagc cagagaagga tcagtccaat
     2341 ggccgccccg agcgggattt ggtaattctc gatcggcacg cgggtcgcac tcgacttttg
     2401 ctcgacctcc tccagcggct ggatgccttt ttcataaacc gccgcggcgg cgtccgggcc
     2461 aagcgcctga taaaatccgc cggcgatttc cgcgatctcg cgcagccgcg ccgcgtccag
     2521 gcgcgtcatg acgggcttgc cgcccgaatc ccggacgaaa tccgccttgc cctccggcgt
     2581 ccgcagggga atgagggatc cctcttctga tccgacgccg accgtcagaa tccggatgtg
     2641 cttctcggcc gcgcggtggg ccgccgccaa accgtccgca tccagctcct cgccgtcggt
     2701 gacgatcacc agcgcccgcg tcacgccttc ggcatcgcca aaggccttga gggcggtatc
     2761 aatggccgcc gcgagattcg tgccgccgcg gggaatcaaa tccgtgtcca attcatcaat
     2821 ggtcgccagc accgcggtct ggtcctgcgt gagcggcgcc tgcaagaaag cggagcccgc
     2881 gaaggcgacg acccccaccc ggtcgttgcc cagatacccg atcaaatccc gcgcaaacag
     2941 cttggcccga gccaggcggg cgggcagcac gtcggtcgcg agcatcgagc gcgacacgtc
     3001 gaaggccagg atgacatcgc gcccgcgctg cttgatttcc tgctcgatgt agccggtccg
     3061 cggcttcgcc aggcagacga ttcccagcgc gagcgccgac aggatcagca ccgcccgcag
     3121 gaagcgcagc cccacgctga ccgcgccggc cagctgttgt ttcagccgcg gcgaaacgat
     3181 gcgcgcgatc aatttctcgc tccgccgctg gctcagaaag aagagcagcg cgatcacggg
     3241 aagaaccgcc agcgcaaaca gccagaccgg ctcgccaaaa ctgaatttta cggcagtcgt
     3301 ctccatacgg tttgggaaag caaaatctcc agggccaggc aaagcaaccc caagcccacg
     3361 aaccacggga agagatcctg gtattgcgcg
//
LOCUS       DATDAY010000160         2859 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_158341, whole genome shotgun sequence.
ACCESSION   DATDAY010000160 DATDAY010000000
VERSION     DATDAY010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2859)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2859)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2859
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_158341"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..846)
                     /locus_tag="VIS74_03120"
     CDS             complement(<1..846)
                     /locus_tag="VIS74_03120"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HEY5792261.1"
                     /translation="MTGYGRGEAAGAGCRVVVECSSVNRKQLDAAALLPREYAGLEPR
                     IREQISKSVQRGRVQAAVTIQAERAAVGGMLDPVAARLFAQELRALRQELELGGEVTL
                     DQVLRHPAVLRESSAPADSGAVWPVVEKALAQALTAMLKMRREEGRHLGEDLARRIAG
                     ISKLAAKIAKRAPVVPRLHRAQLRKRLQAARLQAEISEARLAEEIALFAERCDVSEEV
                     TRVQSHLKQFSQKLGQAGAQGRELEFLAQELTREFNTTGAKASDAAITKLVVEAKAEL
                     EKIREQ"
     gene            complement(1024..1773)
                     /locus_tag="VIS74_03125"
     CDS             complement(1024..1773)
                     /locus_tag="VIS74_03125"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEY5792262.1"
                     /translation="MKPSLQLGVQSHCFRHFKDNALVAQKVRGLGLDRIELSGAHADF
                     SQPEAFGPVVETYRQAGISIVSLGVQTFVGADSEKAWFECAAAAGARHISAHFQVDSY
                     LKAIPRVRAWCREFGMKAGIHCHGGYTFGGQPDVIRHLLGLGGPEIGVFIDTAWVLQI
                     GPWRGNPVEWAREFAGQITGVHYKDFVFDRRGQWKDVIVGQGNLDLPAFVRALDEGGF
                     EGIAIVEYEGDVENPEPALQACVEAMRKLAG"
     gene            complement(1884..>2859)
                     /locus_tag="VIS74_03130"
     CDS             complement(1884..>2859)
                     /locus_tag="VIS74_03130"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEY5792263.1"
                     /translation="LASVHAVQDMNAARVGELCASCGIPKACATAAELLADPEVDAVY
                     IAVPNKFHVPLAIQALEAGKHVILEKPFAMNYAEALLAAEAARKCGRVLTLGMNQRFG
                     QNPQKIKALVEKGVFGEIYHAKAYWLRRSGIPRLGTWFGNRDIAGGGCLYDIGVHLLD
                     LCLYVIGRFDATAVSGNTYTKFGNRGLGEGGWGKSDREDARFDVEDFASAHLRFPDGL
                     SVSLDAAWACHTSQAHRLDVELFGTEAGAQVMDARIFRRDAQLGEYVIAEGFPAAVRY
                     PHGERFHNFINHLLEGEELCVTLDQALAVQRILDGIAESSRLGREVVL"
BASE COUNT          526 a          973 c          859 g          501 t
ORIGIN      
        1 ttgttcgcga atcttctcta gctccgcctt ggcctcgacg acaagcttcg tgatggcggc
       61 atcggacgcc ttcgctccgg tcgtgttgaa ttcacgcgtc aattcctgcg cgagaaactc
      121 cagctcccgg ccctgcgcgc ccgcctggcc gagcttttgg gaaaattgtt tgaggtggct
      181 ctggacgcgc gtcacctcct cgctcacgtc gcaccgctcc gcaaagaggg cgatctcctc
      241 cgccagccgc gcctcgctga tctccgcctg gagccgcgcg gcctgcaggc gcttgcggag
      301 ctgcgcgcgg tgcagccgcg gcaccaccgg cgcgcgtttg gcgattttcg cggcgagttt
      361 ggaaatcccg gcgatgcggc gggccagatc ctcgcccaaa tgccgccctt cctcgcggcg
      421 catcttcagc atcgcggtca gcgcctgcgc cagcgccttc tccaccaccg gccagacggc
      481 gccgctgtcc gcgggagcgg agctctcccg cagcaccgcg ggatggcgca acacctgatc
      541 cagcgtcacc tcgccgccaa gctccagctc ctgccgcaac gcccggagct cctgcgcaaa
      601 taagcgcgcc gccaccggat ccagcatgcc gccgacggcg gcccgttcgg cctggatcgt
      661 gaccgcggcc tgcacgcggc cgcgctggac ggacttggaa atctgctcgc gaatccgcgg
      721 ttcgaggccc gcgtattccc tcggcaaaag cgcggccgcg tccagctgct tgcgattgac
      781 ggacgaacac tcgaccacca cgcggcaacc cgccccggcg gcttcgccgc gtccataacc
      841 ggtcatgctt tgcatccgca gaagctacgc aaagcgaacg acccgtgtcc agtgagccgt
      901 taacggcggg aaagcggaac gaattttgaa cagaaggcca caaaggccgc aaaggatttg
      961 aacattttcc tttgtgatct ttgtggcctt ctgttcaaaa attaaaaaac ccttcctctt
     1021 ccgtcaaccc gccagctttc gcatggcttc cacgcaggcc tggagcgcgg gctcggggtt
     1081 ttccacatcg ccctcgtatt cgacgatcgc gattccctca aatccgccct cgtcgagcgc
     1141 ccgcacgaag gccggcaggt ccagatttcc ctggccgacg atgacatcct tccactggcc
     1201 gcggcgatcg aagacaaaat ccttgtaatg cacgccggtg atctgcccgg cgaattcccg
     1261 ggcccattcg acggggttgc cccgccaggg gccgatctgc aacacccagg cggtatcaat
     1321 gaacacgccg atctccggcc cgcccaagcc gagcagatgg cggatcacgt ccggctgccc
     1381 gccgaacgtg taaccgccgt ggcagtgaat gccggccttc atgccgaact cgcggcacca
     1441 ggcccgcacc cgcgggatcg ccttcaggta gctgtccacc tggaaatgcg cggaaatgtg
     1501 ccgcgcaccg gccgccgccg cgcactcgaa ccaggctttt tccgaatccg cgccgacaaa
     1561 ggtctgcacg ccgagcgaga cgatggaaat gcccgcctgg cgatacgttt cgaccaccgg
     1621 accgaacgct tccggctggc tgaaatcggc atgcgcgccg ctcagctcga tgcggtcgag
     1681 cccgaggccc cgcacctttt gggcgaccag ggcgttatcc ttgaaatggc ggaagcaatg
     1741 gctctgcacg ccgagttgga ggctgggttt cataagagtg ggcattttta accgcaaaga
     1801 acgccgagag cacaaaaaat tggcaagggc attggttctt ttgggttctt tgcgttcttg
     1861 caggctaatc aacggggtgg aaattaaagg acgacttcgc ggcccaaacg ggaggattcg
     1921 gcgatgccgt caaggatgcg ctggacggcc agggcctgat cgagggtcac gcagagttcc
     1981 tcgccctcca ggaggtgatt gatgaaatta tggaaccgct cgccgtgcgg atagcggacc
     2041 gcggcgggaa acccttcggc gatcacatac tcgcccagtt gggcgtcgcg gcggaaaatc
     2101 cgggcatcca tgacctgcgc gccggcctcg gtgccaaaca actccacgtc caggcgatgg
     2161 gcctggctcg tgtggcaggc ccaggccgcg tccagcgaga cgctcaagcc atccgggaag
     2221 cggagatgcg cggaggcaaa atcctccacg tcaaaccggg cgtcctcccg atccgatttt
     2281 ccccagccgc cctcccccag gccgcggttg ccaaacttgg tgtaggtgtt gccggaaacg
     2341 gccgtggcgt cgaaccgccc aatgacgtaa aggcagagat ccagcaggtg cacgccaatg
     2401 tcgtagaggc agccgccgcc ggcgatgtcg cgattgccaa accaagtgcc cagccgggga
     2461 ataccggagc ggcgcagcca atacgccttc gcgtggtaaa tttcgccaaa cactcccttt
     2521 tccaccagcg ctttgatttt ctgcgggttc tggccgaaac gctgattcat gccgagcgtg
     2581 agaacgcggc cgcattttcg cgcggcctcc gccgccagaa gggcctccgc ataattcatc
     2641 gcgaagggct tttcgaggat gacatgcttg ccggcctcca gcgcctggat ggccagcgga
     2701 acgtgaaact tgttcggcac cgcaatgtaa accgcgtcca cctcgggatc ggccagcagc
     2761 tccgccgccg tggcgcaagc cttgggaatt ccgcagctcg cgcacagttc gccgacccgg
     2821 gcggcgttca tgtcctgcac cgcgtgcacg ctcgcgagg
//
LOCUS       DATDAY010000161         3248 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_1589357, whole genome shotgun sequence.
ACCESSION   DATDAY010000161 DATDAY010000000
VERSION     DATDAY010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 3248)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3248)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3248
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1589357"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..674
                     /locus_tag="VIS74_03135"
     CDS             <1..674
                     /locus_tag="VIS74_03135"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HEY5792264.1"
                     /translation="VPAGSLGYWQDRLKARGVSTQAVFPRFGEDVLRFADPDGMIVEL
                     IDSGSAESGPAVQGFHSVSAALAETEGTARLLKDVFGYDSVQESGNRLRLSSPDASAF
                     GSRIDLVRAPDAPPGRAGAGTVHHLAFRVPDDEAQHAWRERLVRLGYPVSPIMDRSYF
                     HSIYFREPGGILFEIATDNPGFAADEPLAELGNKLCLPSWMERSRSRIETLLPPLHLS
                     TAASV"
     gene            671..1294
                     /locus_tag="VIS74_03140"
     CDS             671..1294
                     /locus_tag="VIS74_03140"
                     /inference="COORDINATES: protein motif:HMM:NF014305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY5792265.1"
                     /translation="MKTPTPDFPHVFVPGNGARALLLLHGTGGNETSLIPLGQSLLPG
                     AALLSPRGKVLENGMPRFFRRLAEGIFDEADLIRRTHELADFLIHARKEYGLDPKRLF
                     AVGYSNGANIAGSLLLLRPEVLGGAALLRPMVPLIPEKLPNLPGVPVLISAGNQDPIC
                     ASSEARELADLLRRAGAEVSLEFENAGHGLTEHTVRVAQEWLQQRLP"
     gene            1358..1672
                     /locus_tag="VIS74_03145"
     CDS             1358..1672
                     /locus_tag="VIS74_03145"
                     /inference="COORDINATES: protein motif:HMM:NF018563.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HEY5792266.1"
                     /translation="MEFANVTAIAKANVYFDGKVVSHPLLFPDGSKKTLGLIFPGTYH
                     FGTQAAELMQITDGACEVTLDGQSEPASYSSGTQFEVPADSGFTIKVAEGICQYVCSF
                     LP"
     gene            complement(1713..1871)
                     /locus_tag="VIS74_03150"
     CDS             complement(1713..1871)
                     /locus_tag="VIS74_03150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792267.1"
                     /translation="MKTFTQFIRRIRRPRPTFYQQLELPLRGSRLTRDDVFVLGELQR
                     LREQLAKA"
     gene            1932..2936
                     /locus_tag="VIS74_03155"
     CDS             1932..2936
                     /locus_tag="VIS74_03155"
                     /inference="COORDINATES: protein motif:HMM:NF024676.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HEY5792268.1"
                     /translation="MEKISGGLAAKHSRPPLVRMIRIHESLQGGRQPNCSQLARELEV
                     STKTAQRDIEFMRDQLQLPIEYDAAAHGYFYAEAVTQFPTLTVTQGELVALLVAQKAV
                     RQYRGTPFEKPIASAFEKLAAGMKEQTGVSLHELSEAFSFKPASLAAGELRAFETIAQ
                     AVVKMREIEFDYQSLTGSQAQPRRIRPYHLGCIADQWYVIGFDTAREALRTFALSRTR
                     AVKATARAFERPADFSIQEFLSGSFAAFQAQKIETVKLRLDAFAARLAAERRWHSSQE
                     ITPQTDGFSLLTLKVGLAPDLENWILSWGEHATVLEPEPLVKNIAGKLRVMAEHYGLP
                     "
     gene            2933..>3248
                     /locus_tag="VIS74_03160"
     CDS             2933..>3248
                     /locus_tag="VIS74_03160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792269.1"
                     /translation="MTRKLLFTAAAVGLIGAGLIGYSFTLPFYTDPAAPSRLSSELHP
                     GQIAEWYARLAAYETPRKALFNWGQGLLSLGLAIGTAIGVLGLYLRNPRFRNAEAFFI
                     GWN"
BASE COUNT          555 a         1077 c         1006 g          610 t
ORIGIN      
        1 ccgtccccgc cggctcgctc ggttactggc aggaccggtt gaaggcgcgc ggcgtgtcca
       61 cccaagccgt cttcccgcgc tttggcgagg acgtgctgcg tttcgcggac cccgatggca
      121 tgatcgtgga gttgatcgac agcggttccg cggagagcgg gcctgccgtt cagggctttc
      181 acagcgtctc cgccgcgctg gcagaaaccg agggcaccgc gcggttgttg aaggacgtct
      241 tcggctacga ctccgtgcag gaatccggca atcgcctgcg cctgagctcc ccggatgcct
      301 cggccttcgg cagccggatc gacctcgtgc gggcgccgga cgcgcctccg ggccgcgccg
      361 gggcgggcac cgtgcaccac ctcgcgttcc gcgtgcccga tgacgaggcc cagcatgctt
      421 ggcgcgaacg gctggtccgg ctcgggtatc cggtcagccc gatcatggat cgcagctatt
      481 ttcattcgat ctatttccgc gaacccggcg gcattctctt cgaaattgcc acggacaacc
      541 cgggctttgc cgcggatgag ccgctggccg aattgggcaa caagctctgc ctgccgtcct
      601 ggatggaacg ctcgcgttcg cgtatcgaaa cgctgctgcc gccgctgcac ctttccaccg
      661 ccgcttccgt atgaaaaccc ccacgcccga cttcccgcat gtctttgtcc ccggcaatgg
      721 cgcccgcgcc ctgctcctgc tgcacggcac gggcggcaac gaaacgtccc tgatcccgct
      781 tgggcagtcg ctcctgccgg gtgcggccct gctcagtccc cgcggcaagg tgctcgaaaa
      841 cggcatgccg cgctttttcc ggcggctggc cgaaggcatt ttcgacgagg cggacctgat
      901 ccggcgaacc cacgagctcg ccgacttcct cattcacgca aggaaggaat acggcctgga
      961 tccgaagcgt ttgttcgccg tcggttactc caacggcgcc aacatcgccg gcagcctctt
     1021 attgctgcgc ccggaggtgc tcggcggcgc ggcgctcctg cggccgatgg tgccgctcat
     1081 tccggaaaaa ttgccgaatc ttcccggcgt cccggtgttg atttccgccg gcaatcagga
     1141 cccgatctgc gccagctcgg aggcccgcga actggcggat ctgctccgcc gggcgggcgc
     1201 ggaagtgagc ttggagttcg aaaacgccgg ccatggactc accgagcaca cggttcgggt
     1261 ggcgcaggag tggctgcaac aacgccttcc ctgagcgcaa gggcgcgcgc ggatattcat
     1321 tgagtcgcga tccggcctcg ggcacgatgc cgcgcgtatg gaattcgcca atgtcaccgc
     1381 aatcgccaag gccaacgttt atttcgatgg caaggtggtt tcgcatccgc ttttgttccc
     1441 ggacggctcg aaaaaaacgc tggggttgat tttccccgga acgtatcact tcggcaccca
     1501 ggcggcggag ctcatgcaga tcaccgatgg cgcttgcgag gtcaccctcg acgggcagtc
     1561 ggaacccgcg agttattcca gcggcacgca gtttgaagtg cccgcggact cgggttttac
     1621 catcaaggtc gccgaaggca tctgccaata cgtttgttcg tttttgcctt gatccaagtt
     1681 atgagtggca tcggcgggat gccgatgcca cttcacgcct tcgcgagctg ctcgcggagg
     1741 cgctgcagct cgcccaggac aaagacatcg tcccgggtga ggcgcgaacc ccgcaggggc
     1801 agttcgagct gctgatagaa cgttgggcgc ggccggcgaa tgcggcggat gaattgagtg
     1861 aaggttttca taagtttcgg gtttgacttt accggcacgg atcggacatt tgatgtccga
     1921 tgccaaaaaa agtggaaaaa atttccggcg ggctggccgc caagcattcc cgtccgccgc
     1981 tcgtgcggat gatccgcatc cacgagtcgc tccaaggcgg ccgccagccc aattgctcgc
     2041 agctggcgcg ggagctggag gtttccacga aaaccgcgca gcgcgacatc gagttcatgc
     2101 gcgaccagct ccagctgccg atcgaatacg atgcggcggc gcatggttat ttctacgcgg
     2161 aggcggtcac gcaatttccc accctcaccg tcacgcaggg cgaactcgtc gcgctgctcg
     2221 tcgcccagaa agccgtgcgg caataccgcg gcacgccctt tgaaaaaccg atcgcctccg
     2281 ccttcgaaaa acttgcggcg ggcatgaagg aacaaaccgg cgtgtcgctc catgaactga
     2341 gcgaggcctt ttccttcaaa cccgccagcc tcgcggccgg ggaactgcgg gcgtttgaaa
     2401 cgatcgccca ggcggtggtg aaaatgcgcg agatcgagtt tgactatcaa tccctcaccg
     2461 gcagccaggc gcagccgcgc cgcatccgcc cgtatcatct cggctgcatc gcggatcaat
     2521 ggtatgtcat cggcttcgac accgcgcggg aggcgctgcg gacgttcgcc ctgtcgcgaa
     2581 cccgggcggt gaaggccact gcgagggctt tcgagcggcc cgcggatttt tcgatccaag
     2641 aatttctttc cggcagcttc gccgccttcc aggcgcagaa gatcgagacc gtgaagctcc
     2701 ggctcgacgc cttcgccgcc cggctggccg ccgaacgccg ctggcactcc agccaggaga
     2761 tcacgccgca aaccgatggt ttctccctgc tcacgctgaa ggtcggcctc gcgcccgatc
     2821 tggaaaactg gattctctcc tggggcgaac acgccaccgt gctggagccg gagccgctcg
     2881 tcaaaaatat cgctggcaaa ctccgggtga tggccgaaca ctacgggctg ccatgacccg
     2941 caagcttctc tttaccgccg ccgccgttgg cttgatcggc gccggtttga tcggctattc
     3001 gttcacgctg ccgttctaca ccgacccggc cgcgccctcc cggctgtcca gtgagctgca
     3061 cccgggacag atcgcggaat ggtatgcgcg gctcgcggct tatgaaacgc cgcgcaaggc
     3121 gctattcaac tggggccagg ggctgctcag cctgggcctg gcaatcggga ccgccatcgg
     3181 cgttctgggc ctctacctcc ggaatccgcg ctttcgcaat gccgaagcct ttttcatcgg
     3241 ctggaacg
//
LOCUS       DATDAY010000162         5114 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_793784, whole genome shotgun sequence.
ACCESSION   DATDAY010000162 DATDAY010000000
VERSION     DATDAY010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 5114)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5114)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5114
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_793784"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..224)
                     /locus_tag="VIS74_03165"
     CDS             complement(<1..224)
                     /locus_tag="VIS74_03165"
                     /inference="COORDINATES: protein motif:HMM:NF013468.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sua5/YciO/YrdC/YwlC family protein"
                     /protein_id="HEY5792270.1"
                     /translation="MNTRIVSPDAIGEVVAALERGEPAALPTETVYGLAADALNPLAC
                     AKIFEAKERPLWDPLIVHLPGVGEALVPSA"
     gene            complement(221..715)
                     /gene="purE"
                     /locus_tag="VIS74_03170"
     CDS             complement(221..715)
                     /gene="purE"
                     /locus_tag="VIS74_03170"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEY5792271.1"
                     /translation="MPKTAKALVGVVMGSQSDWSTLREAVQILEEFGVPHEYRVVSAH
                     RTPDLLSEYAKTAAGRGLRAIIAGAGGAAHLPGMLAAHTPVPVLGVPVESKALKGMDS
                     LLSIVQMPAGIPVATFAIGVAGAKNAALFAVAQLAAGDAALAKKLQAFRQRQTDAALA
                     SELS"
     gene            804..1007
                     /locus_tag="VIS74_03175"
     CDS             804..1007
                     /locus_tag="VIS74_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_465540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEY5792272.1"
                     /translation="MARGKVKWFNDKKGFGFITDPEVEGDIFVHFSQIEDQGYRTLKE
                     GEEVEYELYYDEKGSRARKVSRV"
     gene            1508..2041
                     /locus_tag="VIS74_03180"
     CDS             1508..2041
                     /locus_tag="VIS74_03180"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: protein motif:HMM:NF013375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEY5792273.1"
                     /translation="MLRNIVLIGFMGCGKSSVGRRLAGMTGHRFLDTDELIVEKRGQP
                     ISEIFAINGEAAFRDLESAELESLCGVGGIILATGGGIVLREENQARLREIGTVVWLD
                     ARPDLLFERVSRNQKRPLLQTENPRQTFDRLLNARRSIYGATADFRVDSTGLTHDETA
                     QLILEECRRFEGCRIRG"
     gene            2091..3134
                     /gene="gap"
                     /locus_tag="VIS74_03185"
     CDS             2091..3134
                     /gene="gap"
                     /locus_tag="VIS74_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEY5792274.1"
                     /translation="MTKIGINGFGRIGRLVFRAIVEQGLLGKEVQVVAVNDLVPADNL
                     AYLVKYDSTQGKFAGEVYSEKSSPDAPEDDVLVVDGQKIKCLAVKEGPAALPWKELGV
                     DIVIESTGLFTDAEKAKGHLAAGAKKVIISAPAKGEDITIVMGVNHEKYDHANHHIIS
                     NASCTTNCLAPVVHVLLTEGFGVDEGLMTTVHSYTATQKTVDGPSKKDWKGGRSAAIN
                     IIPSTTGAAKAVGLAIPEVKGKLTGMSFRVPTPTVSVVDLTVKTVKETSYKEIAAAMK
                     KASETYLKGVLAYTEDEVVSSDFIHDKSSSIFDAGSGIELNSKFFKLISWYDNEWGYS
                     NRCVDLLKFILSK"
     gene            3257..4501
                     /gene="pgk"
                     /locus_tag="VIS74_03190"
     CDS             3257..4501
                     /gene="pgk"
                     /locus_tag="VIS74_03190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEY5792275.1"
                     /translation="MKKTVRDISLQGKRAFVRVDFNVPQDKATGAITNPQRIVAALPT
                     IKYLLEQGASVVLASHLGRPDGKRVEKYTLKPVAEKLQELLGQPVTFLPDCVGPEVEA
                     ACAALKPGEVVLLENVRYHLEEEGKAKQADGTSLKADPAKVAEFRASLTRLGDVYVND
                     AFGTAHRAHSSVVGVDLPDKVAGFLMEKELTAFAAVIEHPQRPLLAILGGAKVADKIP
                     LIRNLLDQADAIIIGGGMAFTFLKTLDGMKIGESLFDPEGAKIVGELVEKAKAKGVKL
                     YLPVDFLCADKFDPEANTQPATQAEGIPDGWQGLDGGPETAKLFAEAIAEAQTIVWNG
                     PAGVFEFDKFAATTKAMAAAIAEATARGATTVVGGGDTATAAKKFGVVDKVTHCSTGG
                     GASLELLEGKELPGVAALDDKN"
     gene            4588..>5114
                     /gene="tpiA"
                     /locus_tag="VIS74_03195"
     CDS             4588..>5114
                     /gene="tpiA"
                     /locus_tag="VIS74_03195"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEY5792276.1"
                     /translation="MRKKIIAANWKMNMTVEETETFLRDFRLEIEDVTEAEIVIAPPF
                     TALAKLSELLGGSQKIRLGAQNFYYEKSGAYTGEISATMLRELFVRYVIIGHSERRQI
                     FGETDALINKKVHTALASELRPILCVGETLEEREAGQEKAVLETQLQGGLAGVSAEQL
                     LDVVIAYEPVWAIGTG"
BASE COUNT         1082 a         1474 c         1608 g          950 t
ORIGIN      
        1 aaggcgcttg gcacaagcgc ctctccaacg ccgggcaaat gaacaatcaa cggatcccac
       61 agcggccgct ccttggcctc gaagattttc gcgcaggcca gcggattcag cgcatcggcg
      121 gcgaggccgt aaacggtttc cgtcggcagg gccgccggtt ccccgcgttc gagcgccgcc
      181 acgacctccc cgatggcgtc cgggctgacg atccgggtgt tcacgaaagc tcgctcgcca
      241 gcgccgcgtc ggtctgccgc tggcggaagg cctggagctt cttcgcgagc gcggcgtccc
      301 cggcggccag ctgggccacc gcgaagagcg cggcattttt ggctcccgcc acgccgatgg
      361 caaacgtcgc caccggaatg ccggcgggca tttgcacgat ggagagcagc gaatccatgc
      421 ccttgagcgc cttgctttcg accggcacgc cgagcaccgg cacgggggtg tgggcggcca
      481 gcattcctgg caggtgcgcc gcgccgcccg ctccggcaat gatggcccga aggccgcggc
      541 cggccgccgt cttggcgtat tccgaaagca ggtccggggt gcggtgggcc gagacgacgc
      601 ggtattcgtg cggcacgccg aattcttcga ggatctgcac ggcttcgcgc agggtgctcc
      661 agtccgactg gctgcccatg acgactccca cgagggcttt ggcggtcttt ggcatgatga
      721 cgaaattgtt aaaaatgggc gattgaaccc agccgggggc gggtcagtct ttcccggagt
      781 ttatttcgcg aaattcagca cccatggcga gaggaaaagt aaagtggttc aatgacaaaa
      841 agggttttgg gttcatcact gacccagagg tcgaaggcga catcttcgtt catttttcac
      901 agatcgagga ccagggctac cgcacgctga aggaaggcga ggaagtcgaa tacgagcttt
      961 attacgatga aaaaggctcg cgagcgcgga aggtgtcgcg ggtgtgaggc ttatttgctt
     1021 ttttaaaagc gagttaatgt aggggcggtt gtgccaaccg cccggcgtaa acggggatgc
     1081 ggggttcccg tttccccgca aaacaaaggc gcccaatggg gtggtggggc ctcatttccc
     1141 cacccgacaa gcgcctctac aacgcgcttg ggatattgga agcttctttt aagaatcgcg
     1201 ctttccgctc gcttcattct tgaaaccgaa tgccttcctg ccggagcgcg tgaacaagcc
     1261 gtgcgagcgc agaaagtttt cgtgggcgag ccacctttcg tccggagtca tcgcgaagct
     1321 tcgggcgaag agaatccgct cgtcgtcgct cagcccccgg aaccgcttca gacgctttaa
     1381 ctcggcttcg agtttttttt gtccacccgt tgaccctgcc agcttcggcg ggatttgtcg
     1441 agggaaccct ccgcgcctcc cggcgaaaaa ggctcgcgtg cccaccgggt tatgcgagct
     1501 tggaggggtg ctgcgaaata ttgtgctgat tgggttcatg ggctgcggaa aaagttcggt
     1561 cggtcggcgg ctggcgggaa tgaccggcca tcggtttctg gataccgatg agttgatcgt
     1621 cgaaaagcgc ggccagccga tcagtgaaat tttcgccata aatggggaag cggcgttccg
     1681 ggatctggaa agcgccgaac ttgagtcgct gtgcggagtg ggcggcatca tcctcgcgac
     1741 cggcggcggc atcgtgctgc gggaggaaaa tcaggcgcgc ctgcgcgaaa tcggcacggt
     1801 cgtctggctc gacgcgcggc ccgaccttct ctttgaacgg gtttcccgga atcaaaagcg
     1861 gcccctgctg caaacggaaa atccgcgcca gaccttcgac cggctgttga acgcacggcg
     1921 ttccatttat ggggcaaccg ccgattttcg ggtggattcc accggcttga ctcatgacga
     1981 aaccgcgcaa ctcattctgg aggaatgccg ccgttttgaa ggttgcagga ttcgcggttg
     2041 accatcgtgc cccggcgcat tattcgcatt gaggaaacca aacacacatt atgacaaaaa
     2101 tcggtatcaa cggcttcggc cgaatcggca gactcgtctt tcgggccatc gtggaacagg
     2161 gattgctggg caaggaggtt caagtggtgg cggttaatga tctcgtgcct gcggataacc
     2221 ttgcgtatct cgtgaaatac gactccacgc agggcaagtt tgccggggaa gtttacagcg
     2281 aaaaatccag ccccgacgcc cccgaggacg atgtgctcgt ggtggatggc caaaagatca
     2341 agtgtctcgc cgtcaaggaa ggcccggccg cgctgccgtg gaaggaactc ggcgtggaca
     2401 tcgtcatcga atccaccggc ttgttcaccg atgccgaaaa ggccaagggc cacctcgccg
     2461 cgggtgcgaa gaaggtcatc atctccgcgc cggccaaggg cgaggacatc acgatcgtca
     2521 tgggcgtgaa ccatgaaaaa tacgatcacg cgaatcacca catcatttcc aacgcgagct
     2581 gcacgaccaa ctgcctggct ccggtcgttc acgtgctgct cacggaaggt ttcggcgttg
     2641 atgaaggcct catgacgacc gttcacagct acaccgccac gcagaagacc gtggacggtc
     2701 cctccaagaa ggactggaag ggcggccgtt ccgcggcgat caacatcatt ccgagcacga
     2761 ccggcgccgc caaggccgtg ggcctggcca ttccggaagt caagggcaag ctcaccggca
     2821 tgtccttccg cgtgccgacg ccgaccgtct ccgtggtgga cctgaccgtg aagaccgtca
     2881 aggaaaccag ctacaaggaa atcgccgcgg ccatgaagaa agccagcgag acctacctca
     2941 agggcgtgct cgcctacacc gaggacgaag tggtttcgag cgacttcatc cacgacaaga
     3001 gctccagcat cttcgacgcc ggcagcggca tcgagctgaa cagcaagttc ttcaagctca
     3061 tcagctggta tgacaacgaa tggggttact ccaaccgttg cgtggacctc ttgaagttca
     3121 tcctgtcgaa ataaaataac gaataaccac agaggacacc gagagcactg agttaagaag
     3181 gcggaaaaac tcgcattttt tcggtaattc ttccctctgt gttcccggtg ccctctgtgg
     3241 ttaaaatttt taatccatga aaaagaccgt tcgcgatatt tcgcttcagg gaaaacgcgc
     3301 gtttgtccgc gtggacttca acgtcccgca ggacaaggcc acgggagcca tcaccaatcc
     3361 gcagcggatc gttgcggcgc tgcctaccat caaatatttg ctggagcagg gagcctcggt
     3421 ggtgctggcc agtcatctgg ggcggccgga cggcaagcgg gttgaaaaat acacgctcaa
     3481 gccggtcgcc gagaagcttc aggagctgct cggccagccg gtgaccttcc tgccggattg
     3541 cgtcgggccg gaagtggaag cggcgtgtgc ggcgctcaag ccgggcgaag tggtcctgct
     3601 ggaaaacgtc cgttatcacc tggaggagga aggcaaggcc aagcaggcgg acggcacttc
     3661 cctcaaagcc gatccggcga aggtggcgga gttccgcgcc tcgctcaccc ggctgggcga
     3721 tgtgtatgtg aacgacgcct tcggcacggc gcaccgcgcc cattcgtcgg tcgtcggcgt
     3781 ggatttgccg gacaaggtgg cggggttttt gatggaaaaa gaactgacgg ccttcgccgc
     3841 ggttatcgag catccccagc gcccgttgct ggccatcctc ggcggcgcca aggtggcgga
     3901 caaaatcccg ctcatccgca acctgctcga ccaggcggat gccatcatta tcggcggcgg
     3961 catggcgttc accttcctga agacgctgga tggcatgaag atcggcgaaa gcctttttga
     4021 tccggaaggc gcgaagatcg tgggcgaact cgtcgaaaag gccaaggcca agggcgtgaa
     4081 gctctatctg ccggtggact tcctttgcgc cgataaattc gacccggaag ccaataccca
     4141 gccggccacc caagccgagg gcattccgga tggatggcag gggctcgacg gcggtcccga
     4201 gacggcgaaa ttgttcgcgg aggcgatcgc cgaggctcag accatcgttt ggaacggtcc
     4261 ggcgggcgtg tttgagttcg ataaatttgc ggccacgacc aaggccatgg cggcggcgat
     4321 tgccgaagcc acggcgcgcg gcgcgaccac ggtggtgggc gggggagaca ccgcgacggc
     4381 cgccaagaaa ttcggcgtgg tggacaaggt cacccattgt tcgaccggcg gcggcgcctc
     4441 gctggaactg ctcgaaggca aggagctgcc cggagtggcg gcgctcgacg acaaaaacta
     4501 aactggattt ccggattgaa aattgagact taactcctgt gaatccggca aatccagtta
     4561 atcccgttta gctcaaagaa agaaaccatg cgcaaaaaaa tcatcgcagc gaattggaaa
     4621 atgaacatga ccgtcgagga gacggagacg ttcctgaggg atttccggct ggaaatcgaa
     4681 gacgtgaccg aggcggaaat cgtcattgcc ccgcccttca ccgcgctggc caaactttcc
     4741 gaattgctcg gcggctcgca aaaaatccgg ctcggcgccc agaactttta ttacgaaaag
     4801 tcgggagcct acaccggcga gatcagcgcg acgatgctgc gcgagctttt cgtgcgttac
     4861 gtgatcattg gccacagcga acgccggcaa atctttggcg agacggatgc gctcatcaac
     4921 aaaaaggtgc acaccgcgct ggcctccgag ttgcggccga tcctttgcgt gggggaaacc
     4981 ctggaggagc gcgaggctgg ccaggaaaag gcggtgctcg aaacccagtt gcagggaggc
     5041 ctggcgggag tcagcgccga acagctgctg gatgtggtca ttgcttacga acccgtttgg
     5101 gccatcggca ccgg
//
LOCUS       DATDAY010000163         4552 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_2043963, whole genome shotgun sequence.
ACCESSION   DATDAY010000163 DATDAY010000000
VERSION     DATDAY010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 4552)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4552)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4552
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2043963"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..649
                     /locus_tag="VIS74_03200"
     CDS             <1..649
                     /locus_tag="VIS74_03200"
                     /inference="COORDINATES: protein motif:HMM:NF016060.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HEY5792277.1"
                     /translation="PLSDPMSGFFVLRRRVFEESVRRLSAKGFKILLDIVLSSPRPLK
                     LAEVPYTFRTRQHGESKLDAVVAFEYLMLLIDKLVGRFIPVGFLLYTLVGASGVVLHL
                     AVLALLFRGMGWGFEAAQAGATFVAMLSNFAFNNLLTFRSSRLKGAAFLPGLALYLAV
                     CGVGAICNVQLAEYLFQHGIPWWVAGTIGGLVGAVWNYAVSAHLIWTWLRQRFTR"
     gene            697..2493
                     /locus_tag="VIS74_03205"
     CDS             697..2493
                     /locus_tag="VIS74_03205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792278.1"
                     /translation="MNEGNPAAPSDGLNRAAGWIVAGMCALFTWHVLAWSMEFGKLSV
                     PPDYDDSHSLVEGAVRSATLRAHGPGAAVQEYLTRMPHSFLHYYLAAGLFAGFGVDQR
                     VPYWANGLLLLGVLLTFVRQLPPLPALQKLGFVAAFLGVPISFHLIHDFRSECTMAGF
                     LFIACGAAVQWVWAEKSAGRWLALAAVFFALAFAVKPVMFPYVGGMLGLCSLLAVGVN
                     WLHPRRKWTLLARQLALLWGISLLPFLCHFYLFREQILKYIWNVAFASDFYKLSASGG
                     ARWLFHWQGYSGVWHLSWVKTVFAAIAASGVLLGCIPHLRGYAPGKIGLSYAFLTAGS
                     FLGIAINAVNQPFFGMTFQLLLLSTALLTLAHYYSRWEQPWIVAGITLLLGLYWLKAT
                     LTLSFLIPAALVIALGAGLCWLTRLPGKQWVGVLGSGMLALYCWKVTQIAPYQNYRDR
                     TAAEAGPAGLVWRQEGPKQAYEILKPFWNPARPPLVWVSAYGWVDGNTIGWEAAKDGK
                     GWKIYNRGELPIDPDKKFPTQAEIIIVPEAGITGELATPDLSEAAALPDRLRADPDFL
                     LLAEIAASNGKKIQVFQRRISRQAADPASLGH"
     gene            2506..4110
                     /locus_tag="VIS74_03210"
     CDS             2506..4110
                     /locus_tag="VIS74_03210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792279.1"
                     /translation="MATSRSLGLSGLCLLIALILTGVVVSYSSLHGKLAIPPDYDDSH
                     SLVEGGLRLLTFQNNGLEAAWHEYLTRLPHSFLHYYFAALCMGIGGISEPVVYWANGI
                     FLFAALLGLTSLLSSLPLLRSIPLIAAFACVPAAFNLVFDFRSECAMAALLFAGCCVL
                     IRTIWAPPRFLISGAGITGLLFALCLGLKPAMFPYIIGMAGAGTLLWFGIGWRRQKWR
                     EIILPAAVLWLISVVPFAFHYVLNAEQIFGYILFNAFQSDFWKQGGSLYEQLTFHFAG
                     FPGKFQLGIFAWPLLFLVLISNILAIQQRKAGDSLLRRYLFSASFLTLFAYLGVAVNS
                     MNQNYFGMTFHFLLTASALLGLAWAATLPPGKVGTGLCWAALGLALIAWKIPVSQDYV
                     ARTRAVGGDAALMWRREAPAHLLEIVQPYWREASPPKAWVAAYGWVDGNTISWEAIRK
                     GLPWKFWNYYENALPPEESFPSWGEILVVPEPGVMGTIDLPSVKALPQILSDIEKNPN
                     IQQIGQITDPTGKAVRVFRRMNEISH"
     gene            4200..>4552
                     /locus_tag="VIS74_03215"
     CDS             4200..>4552
                     /locus_tag="VIS74_03215"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY5792280.1"
                     /translation="MKFSIVTPSFNQASHLEGTLRSVLDQPGVDLEYLVVDGGSTDGS
                     REVIERHAGRLAWWCSERDGGQYAAINKGFARTTGDILGWLNSSDLYLPWTLITVQQI
                     FTQFPEVQWITSLRKL"
BASE COUNT          728 a         1336 c         1372 g         1116 t
ORIGIN      
        1 gccgctgagc gatccgatga gcggattttt tgtgctccgc cgccgggtct tcgaggagtc
       61 ggtgcgccgg ctctccgcga aggggttcaa gattctcctg gatatcgtgc tctccagccc
      121 ccgtcccctg aagctggcgg aggttcccta cacgttccgc acgcggcagc atggcgaaag
      181 caagctggat gcggtcgtgg cgttcgaata tctcatgctc ctcatcgaca agctggtcgg
      241 ccggttcatc ccggtggggt tcctgcttta cacgctcgtg ggagccagcg gcgtggtgct
      301 ccacctggcc gtgctggcgc tgctctttcg gggaatgggc tggggttttg aagcggccca
      361 ggccggagcg accttcgtcg cgatgctttc gaattttgcc ttcaacaatc tcctgacgtt
      421 tcgctcctcc cggctgaagg gggcggcgtt cctgccgggg ctggccctgt atctggcggt
      481 ctgcggagtg ggggcgatct gcaacgtcca gcttgcggaa tatctttttc agcacggcat
      541 tccctggtgg gtagcgggaa caatcggcgg attggtcgga gcggtctgga attacgccgt
      601 ttccgcccac ctcatctgga cgtggctgcg gcagcggttt acgcgctgag ggattcggga
      661 aacggcggaa aaactcgggc gaggagagat gaatcaatga atgagggaaa cccagccgcg
      721 ccttcggacg gcctgaatcg cgccgcgggc tggatcgtgg ccgggatgtg cgccctgttt
      781 acctggcacg tgctggcttg gtccatggag ttcggcaaac tttccgttcc gccggattac
      841 gacgacagcc atagtttggt ggagggggcc gtccgttcgg ccaccttgcg ggcgcacggc
      901 ccgggggctg cggtgcagga atacctcacc cgcatgccgc attcgttcct gcactactac
      961 ctggccgccg gtttgtttgc cggatttggc gtggaccagc gcgtgcccta ttgggcgaat
     1021 ggcctccttt tgttgggagt gctgctgact tttgtccgcc agcttccccc gctgccggcc
     1081 ctgcaaaaac tcgggtttgt ggctgccttc ctgggggttc cgatctcctt tcatctcatc
     1141 catgatttcc gctcggaatg cacgatggcg ggttttctct tcatcgcttg cggggcggcg
     1201 gtgcaatggg tctgggcgga gaaatccgcc gggcgctggc tggcgctggc ggcggttttc
     1261 tttgccctcg cctttgccgt caagccggtc atgtttccct acgtgggcgg aatgctcggg
     1321 ttatgctccc tgctggcggt gggagtgaac tggctgcacc cgcggcgcaa atggacgttg
     1381 ctggccaggc agctcgccct gctctggggg atcagcctgt tgcccttcct ctgccacttt
     1441 tatttgttcc gggaacaaat cctgaaatac atctggaatg tcgcctttgc ctcagacttc
     1501 tacaaactgt ccgccagcgg cggcgcccga tggcttttcc actggcaggg ttactccggt
     1561 gtctggcatt tgagctgggt gaagacggtc ttcgcggcca ttgccgcttc gggcgtactg
     1621 ctgggatgca tcccccacct tcgcggttat gcccccggaa aaatcgggtt gtcctacgcc
     1681 tttctgaccg ccggctcctt tctgggaatc gccatcaatg cggttaacca gccttttttc
     1741 gggatgacct tccagttgct gctgctcagc acggccctgc tcacgttggc ccattattat
     1801 agccgatggg aacagccctg gattgttgcc ggcatcaccc tgttgcttgg cctctattgg
     1861 ttgaaggcaa ccctgacttt atccttcctc attccggcgg cgctggtcat tgctttgggc
     1921 gccgggctct gctggctgac ccggttgccg gggaagcagt gggtcggagt gctcggctcg
     1981 ggaatgctgg ccctctattg ctggaaagtc acccagatcg ctccctacca gaattaccgg
     2041 gatcgcacgg cggccgaagc cggtccggcg gggctggtct ggcgccagga aggcccgaag
     2101 caggcatatg aaatcctcaa gcccttctgg aatcccgccc gcccgccgct cgtctgggtg
     2161 tccgcctacg gctgggtgga tggcaacact atcggatggg aggcggcaaa ggatggaaag
     2221 ggctggaaaa tctataatcg cggggaattg ccgatcgacc cggacaaaaa gtttccgacg
     2281 caggcggaaa tcatcatcgt tcccgaggct ggcatcacgg gcgaactggc cactcccgac
     2341 ctcagcgaag ccgccgcgct gcccgatcgt ctgcgggccg atccggattt tcttctcctg
     2401 gcggagatcg cggcgtccaa cggcaaaaaa atccaggttt ttcagcgccg aatttcccgg
     2461 caggcggccg accccgcctc tctgggccac tgatcatttt ccctgatggc gacgtcgcgt
     2521 tctctcgggc tctccggctt gtgcttgttg attgcgctca tcttaaccgg cgtggtggtc
     2581 agctattcca gcttgcatgg caagctggcg atcccgcccg attatgatga cagccatagt
     2641 ttggtggaag gcgggctccg attgctcacc ttccaaaata atgggttgga ggcggcatgg
     2701 cacgaatact tgacccgcct gcctcactcc tttctccatt attattttgc cgccctttgc
     2761 atgggcatcg gcgggataag tgagccggtg gtttactggg ccaacgggat ctttttgttc
     2821 gcggcattgc ttggattaac cagtcttctc tcctcgctgc cgcttctccg gtccatcccg
     2881 ctgattgcgg cattcgcctg cgtgccggca gcctttaatt tagtttttga tttccggtcg
     2941 gaatgcgcca tggccgcctt gctgtttgcc ggctgctgcg tcctcatccg gacgatctgg
     3001 gctcccccga ggtttttaat ctccggggcg ggcatcaccg gccttctttt tgccctctgc
     3061 ctggggctca agccggccat gtttccctac attatcggca tggctggagc ggggacgctc
     3121 ctttggttcg gaatcggatg gcgccggcaa aaatggcgtg aaataatcct gcccgccgcc
     3181 gtcctgtggc taatttcggt tgttcccttt gcctttcact acgtcctcaa cgccgaacag
     3241 atctttggct acattctctt caatgcgttt caatccgatt tttggaaaca gggaggcagc
     3301 ctttatgaac agctcacctt tcacttcgct ggattcccgg gaaaatttca acttggaatt
     3361 tttgcctggc cgcttttgtt cctcgtcctg atttccaata ttttggcgat tcagcaaaga
     3421 aaagccgggg attccctttt gcgacgctat ctgttttcgg cttcttttct tacgctgttc
     3481 gcctatctgg gcgtggcggt caactcgatg aatcaaaact acttcgggat gacgtttcat
     3541 tttcttttga ccgcttccgc cctgctgggt ttggcctggg cggccactct gccgccgggc
     3601 aaagttggaa cgggactatg ttgggcggct ctcgggctgg ctctgatcgc gtggaaaatt
     3661 ccggtctcgc aggactatgt tgcccgcacc cgcgcggtcg gcggcgatgc cgccctgatg
     3721 tggcggcgag aggcgcccgc gcatctcctt gagatcgtcc agccctattg gcgggaggcc
     3781 tcgcctccca aggcttgggt cgcggcttac ggatgggtgg acgggaatac gattagttgg
     3841 gaagcgatcc gcaagggatt gccgtggaag ttttggaact attatgaaaa tgctctgccg
     3901 ccggaagagt cctttccaag ctggggcgag attctggtgg tgccggaacc cggagtgatg
     3961 ggaaccattg atctgccttc ggtgaaggca ctgccacaaa ttctgagcga catcgaaaaa
     4021 aacccgaata tccagcagat tggccagata accgatccca caggcaaagc cgttcgggtt
     4081 tttcggcgga tgaatgagat ttcccattga gccccctcgt tttccgacgc tatttattcc
     4141 ggtcttcggg ctggctggcc gccaccgccg ggcttaacct cattgttttt taggctgcta
     4201 tgaaattttc catcgtcact ccgtccttca atcaggcgtc ccacctcgag ggcaccctgc
     4261 gcagcgtcct ggatcagccg ggtgtggacc tcgagtattt ggtggtggat ggcggctcca
     4321 ccgatggcag ccgggaagtc atcgaacgtc acgccggccg gctggcgtgg tggtgcagcg
     4381 agcgcgacgg cgggcaatat gcggccatca acaagggctt tgcccggacc acgggcgaca
     4441 tcctgggctg gctgaattcc tcggatctct acctgccatg gaccttgatc acggttcagc
     4501 agattttcac ccagtttccc gaagtgcaat ggatcacttc gctgcggaaa ct
//
LOCUS       DATDAY010000164         2238 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_2372512, whole genome shotgun sequence.
ACCESSION   DATDAY010000164 DATDAY010000000
VERSION     DATDAY010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2238)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2238
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2372512"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(66..434)
                     /gene="rplT"
                     /locus_tag="VIS74_03220"
     CDS             complement(66..434)
                     /gene="rplT"
                     /locus_tag="VIS74_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEY5792281.1"
                     /translation="MPRATNSPASRARRKRTIKEAKGYRGRRSKLFRYAKDARMKGKY
                     WSYRDRKTRKRNFRALWITRLSAAVRAQGLTYSRFIEGLKAASIGLDRKVLADLAVND
                     ETAFAQIVAKAQDALKAKAA"
     gene            complement(562..771)
                     /gene="rpmI"
                     /locus_tag="VIS74_03225"
     CDS             complement(562..771)
                     /gene="rpmI"
                     /locus_tag="VIS74_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEY5792282.1"
                     /translation="MPRSIARSKTRKSVAKRFKLTATGKVKRSHGGKRHLLNSKSAKR
                     KRRLAQSTLVSDTVTARIKECLPFH"
     gene            complement(1074..>2238)
                     /locus_tag="VIS74_03230"
     CDS             complement(1074..>2238)
                     /locus_tag="VIS74_03230"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: protein motif:HMM:NF015531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HEY5792283.1"
                     /translation="KAEVIDLKGSDGKTYRIGWITLPSFYANMDRSDGSSAKSTTEDV
                     ARLLTRLNAEGIEGLVMDLRRDGGGSLEEAIRLTGLFIPSGPVVQAKDGNGRVTVSRD
                     DESKVAYSGPMVVLANRLSASASEIFAAALQDYGRAVVVGDERTFGKGTVQQVIDVGR
                     FMPFFSLAAADAGSLKLTVQKFYRVKGGSTQLNGVASDIVLPSLTDTPEIGESSMPNA
                     MPYDEVAARPIDRFLGHSLYLDELRRRSQERVGQEPEFRYYQDDIARLKKRLEENRIS
                     LNKSSREAELAADKKRGADRNAERKARGPVLAATAYELTLDTVEAPKLQQVAFDRKPK
                     RSSYLDAEEEPDAKKEDGAEDIVTPDPIRNEALCIAEDLINLSGVKKTAKANN"
BASE COUNT          374 a          674 c          684 g          506 t
ORIGIN      
        1 cagaattaac agaattcaga aaatttaatt ctgaagattc tgttaattct gtcgaagatt
       61 ccgggttagg cggccttggc cttgagggcg tcctgggcct tggcgacgat ctgcgcgaag
      121 gcggtttcgt cgttgacggc caagtcggcg aggaccttgc ggtcgagccc gatgctggcg
      181 gccttcagcc cttcgatgaa gcggctgtag gtgagtccct gcgcgcggac ggcggcgctg
      241 agacgggtga tccacagggc gcggaaattg cgcttgcggg tcttgcgatc gcgatacgac
      301 caatatttgc ccttcatgcg ggcgtccttg gcatagcgga acagcttgga acggcggcca
      361 cggtatccct tggcctcctt gatggtgcgt ttgcgccggg cgcggctggc cggcgagttg
      421 gttgctcttg gcatttatct aaccccgcga tttctcgcgg agcctccggg aacagattgt
      481 tggttggttg ggatccggag cctcacctgt tccgttatcc ccagcctttc gccggggcag
      541 gctcacgaat cctttatcgg atcaatggaa tggcaagcat tccttgatcc gggcggtgac
      601 ggtgtcactc accagggtgg actgcgcaag ccgacgcttg cgcttggcgc tcttgctgtt
      661 gagcaagtgg cgctttccgc catggctacg cttcaccttg ccggtcgccg tcagcttgaa
      721 gcgtttggca accgacttgc gcgttttgga acgtgcgatg gatcttggca taagaggggt
      781 taaactagcc gaagtttcgg aattgggaag cgcaaaattg gggaattttt agccaattcc
      841 ccccatttcg ggcgctttcc cggacaaccg cttatcgtat ttcgcaaaag agatttccaa
      901 aatacgattc aatgtagggg cggttgtgcc aaccgcccag cgtaaacggg gatgcggggt
      961 ccccgtttcc ccgcaaacaa aggcgcccaa tggggtggtg ggaccccatt ccccacccga
     1021 caaccgcctc tacaacccgt tgggtatttc ggaaatctct tttgagaatc gctctaatta
     1081 ttcgccttgg cggttttttt gacgccgctg agattgatca gatcctcggc aatgcagagg
     1141 gcttcattgc gaatcgggtc gggagtcacg atgtcctccg cgccgtcctc ctttttcgca
     1201 tccggctcct cctccgcgtc gagatagctg cttcttttcg gtttgcggtc gaaggccacc
     1261 tgctggagtt tgggcgcttc caccgtgtcg agcgtgagct cgtaggcggt ggcggccagc
     1321 accgggccgc gggccttgcg ttcggcattc cgatcggccc cccgtttttt atccgcggcc
     1381 agttccgcct cgcggctgga tttgttcaac gaaatccggt tctcctccag ccgctttttc
     1441 agccgggcga tgtcgtcctg ataataccgg aactccggct cctggccgac gcgttcctgc
     1501 gaacgccggc gcagttcgtc gaggtaaagc gagtgcccca ggaagcggtc aatcggccgg
     1561 gccgccacct cgtcgtaggg catggcgttg ggcatgctgc tttcgccgat ctccggggtg
     1621 tcggtcaatg acggcagcac gatgtcggag gccacgccgt tgagctgcgt ggacccgccc
     1681 ttgacgcgat agaatttttg caccgtcagc tttaatgagc ccgcatccgc cgcggcgagg
     1741 ctgaagaacg gcatgaagcg gcccacgtcg atgacctgct gcacggtgcc cttgccgaag
     1801 gtacgctcat cccccacgac caccgcccgg ccgtagtcct gcagcgcggc ggcaaaaatt
     1861 tcgctcgcgg aagcgctcag gcggttggcc agcaccacca tggggccgct gtaggcgacc
     1921 ttgctttcat cgtcccggct gacggttacg cggccgttgc catccttggc ctgcacgacc
     1981 gggccggagg gaatgaacaa tccggtcaaa cgaatcgcct cctccaacga tccgccgcca
     2041 tcccggcgca gatccatcac gagcccttcg atgccttcgg cgttcaggcg ggtgaggagc
     2101 cgggcgacgt cttcggtcgt gctcttcgcg ctggagccgt cgctgcggtc catgttcgca
     2161 tagaaggacg gcagcgtgat ccagccgatg cggtaggttt tgccatcgct tcccttcagg
     2221 tcaatcacct cggccttc
//
LOCUS       DATDAY010000165         2400 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_939577, whole genome shotgun sequence.
ACCESSION   DATDAY010000165 DATDAY010000000
VERSION     DATDAY010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2400)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2400)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2400
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_939577"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1747)
                     /locus_tag="VIS74_03235"
     CDS             complement(<1..1747)
                     /locus_tag="VIS74_03235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792284.1"
                     /translation="MKSKITFLSTLAAAVTALLNVHAESADSPVPLYVNQSGYDLGWP
                     KRFTAPTLPDGTRFVVRKKDGGSALFAGTIAGNRGDFSAFEPADPGEYVVEAEGLVSV
                     PFSIAPWWIERVTYQNAVDFMIESRNHLGTHKLPRPLSYGWRDDHAFAFEVNTLVAQY
                     FSNPDAYRRMPHQITYEKPAPEQVAFWGALEPYQEGAPDIVKLIHWGTDVIVTQNLTH
                     ELFKEQAAWFLYAWPQLKEWIPEAAYRTALRFALDNWGENTIALKYPYNGSRGHDLFA
                     LKNRHGGTKGELPPGHSLQPNLFMYEVAKREKLPGVEKFFDAAYRQCEWQIQNLDWND
                     PLVTKGQRMSEHITIPGMVYFLQTYPDRAPKGLREKIEEWAAVAVRRSQNLWDFRKLD
                     DGDGWVPKGEAKNMWNEPGNVAGFPACALAAAEVVPDPELRRQLVILAAAHLDDVFGR
                     NPLGRHFSHDAAKEIEGVERGWPTEYPGGNGMLQPVRFVLDGAPKQEHYPYHPEAGPG
                     YTEGWVNFNTAYNASLAWMAYARTSVALFDAGFAKPLATLSPGETLGIELIAPINFDY
                     ARTEEAAVRVAASSGD"
     gene            complement(1813..>2400)
                     /locus_tag="VIS74_03240"
     CDS             complement(1813..>2400)
                     /locus_tag="VIS74_03240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792285.1"
                     /translation="LDGVKNLAVRNCFIRCEDDGFGWHAVDAKANGEPVTENCLAEDC
                     VIWNTGFGNGLRVGASLETQLFRNITFRNIDVLRHADAAIRSDHSDWARCENIRFENF
                     AVESPGKTVEIVIAQTHYSNNNGYRDERGHFDGLHFINVTAPAGTIELRGFDADHRID
                     RVTFENCSLGGQPIDGPEDITLNEFVTEVSFSHRP"
BASE COUNT          427 a          740 c          767 g          466 t
ORIGIN      
        1 cgtcgccgct gctggcggcg acccgcacgg ccgcttcctc ggtccgggcg taatcgaagt
       61 tgatcggcgc gatgagctcg atcccaagag tctcgccggg cgaaagggtg gcgagcggct
      121 tggcgaaacc cgcgtcgaaa agcgcgaccg acgtgcgggc ataagccatc caggccaggc
      181 tcgcattgta ggcggtgttg aaattcaccc agccctcggt gtagcccggc ccggcttcgg
      241 ggtgatacgg gtagtgctcc tgcttgggcg cgccgtcgag gacgaagcga accggctgca
      301 gcatgccatt tccgccggga tattcggtgg gccagcccct ttcgactccc tcgatttcct
      361 tcgcggcatc gtgcgaaaaa tggcgcccca gcggattgcg gccgaacacg tcgtcaaggt
      421 gcgcggccgc caaaatcacc agctgccgcc gcaactccgg gtccggcacg acctcggccg
      481 ccgcgagcgc gcaggccgga aagccggcga cgttgccggg ttcgttccac atgtttttcg
      541 cctcgccctt gggcacccag ccatcgccgt cgtccagctt gcggaagtcc cacaggttct
      601 gcgagcgccg caccgcgacg gcggcccatt cctcgatttt ttcccgcagg cccttcggcg
      661 cgcgatccgg ataggtttgc aggaaataga ccatgccggg aatcgtgatg tgctcgctca
      721 tgcgctggcc cttggtcacc agcgggtcgt tccaatcgag attctggatt tgccactcgc
      781 actggcgata cgccgcgtca aaaaactttt cgaccccggg cagtttttcg cgtttcgcca
      841 cctcgtacat gaacaggttc ggctgcaggg aatggccggg cggcagctcg cccttggttc
      901 ctccatggcg atttttcaag gcaaacaaat cgtgcccgcg gctgccgttg taagggtatt
      961 tgagcgcgat ggtgttctcg ccccagttgt ccagggcgaa gcgcagcgcc gtgcgatagg
     1021 cggcttccgg aatccactcc ttaagctgcg gccaggcgta aagaaaccag gccgcttgtt
     1081 ccttgaagag ctcgtgagtc aggttttggg tgacaatgac atccgtcccc caatggatga
     1141 gcttcacgat gtcgggcgcg ccttcctgat agggctccag cgcgccccaa aatgccacct
     1201 gctccggcgc cggcttttca taagtgatct ggtgcggcat gcgccggtag gcgtccggat
     1261 tggaaaaata ctgcgccacg agcgtgttca cctcgaaggc aaaggcgtgg tcgtcccgcc
     1321 agccatacga caatgggcgc ggcagcttgt gcgtgccgag gtgattgcgg gactcgatca
     1381 tgaaatcgac cgcgttctgg taggtcaccc gctcgatcca ccacggggcg atggaaaaag
     1441 gcaccgagac caagccttcc gcttccacga catattcgcc cggatcggcc ggctcgaacg
     1501 cggaaaaatc gccccgattc ccggcgatcg tgccggcaaa caatgcgctg ccgccatcct
     1561 tcttccgcac cacgaaccgg gttccgtccg gcagcgtggg agcggtgaaa cgcttcggcc
     1621 agccgagatc gtagccgctc tggttcacgt agagcggcac gggggaatcc gcggactcgg
     1681 cgtggacatt taggagagcc gtcacggccg cggcgagggt tgagaggaag gtgatcttgc
     1741 ttttcatcga cttcggaggg aggggagtta gtccggcgcc cggcggggcc atttccacga
     1801 atgaggcgtc tttcatgggc ggtgggaaaa tgaaacctcc gtgacaaact cattgagggt
     1861 gatgtcctcg ggaccgtcga tcggctggcc gccgagggag caattttcaa aggtcacgcg
     1921 gtcgatgcgg tgatcggcgt cgaatccccg gagctcgatg gtccccgccg gcgcggtgac
     1981 gttgataaaa tggaggccgt cgaaatggcc ccgctcgtcg cggtagccgt tgttgttgct
     2041 gtagtgggtc tgcgcgatga cgatttcgac cgtcttcccc ggcgactcga cggcaaagtt
     2101 ttcaaaccgg atgttttcgc accgggccca gtccgagtga tcgctacgga tggcggcatc
     2161 ggcatgcctg agcacgtcga tgttgcggaa agtgatgttc cggaacagct gggtttccag
     2221 ggaggcgccg acccgcaggc cattgccgaa gccggtgttc cagatgacgc agtcctcggc
     2281 caggcagttt tcggtgaccg gctcgccatt ggctttcgca tccaccgcgt gccagccgaa
     2341 gccgtcgtcc tcgcagcgga tgaaacaatt gcgcactgcc aaattcttca cgccgtcgag
//
LOCUS       DATDAY010000166         3214 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_1941789, whole genome shotgun sequence.
ACCESSION   DATDAY010000166 DATDAY010000000
VERSION     DATDAY010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 3214)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3214)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3214
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1941789"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..708
                     /locus_tag="VIS74_03245"
     CDS             <1..708
                     /locus_tag="VIS74_03245"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HEY5792286.1"
                     /translation="WIDAHEVTIGQYAEFLDWVKKNPNRLVDIAHPKAPKGKDYIPDD
                     WADQTQIDMPGYYSRALKWGKYKEADLDLNSPVFGVDFFDACAYAKWKGRRLPTELEW
                     EKAARGTKGNLYPWGNEPNPKWVNSGADFKPNPKEGGGVDGWKKWNPVDAVGKDKSEF
                     GVMDLAGNVSEWTDSWVPSEDPALGGMQVPVIRGGNWRAEDYKLTRRSTALMAEKPDW
                     RVGFRTASEKAPDKPAN"
     gene            716..1654
                     /locus_tag="VIS74_03250"
     CDS             716..1654
                     /locus_tag="VIS74_03250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792287.1"
                     /translation="MRFRLFLIAWLAGGAALHAQVRVEVSVNRGLYIRYEPIVATVSL
                     TNLSGRELELSDDGSQPWFGFQIQTSDGRPVSTRTAGYQLNPAQVGPGQSLTRQINLT
                     PIYAMDQFGGYRIRASVFLKSDRQFYSSPPISLEITDGRQVWQQKVGVPVGQPDEGTT
                     RTITLLAHRLPQSTALYIRIEDPERGRIFCTHRIGRFVNFGPPTVELDSQNQVHILQL
                     LAPRNYLYSHFGLNGEVLDRKLYTSTGTPPRMQRTPDGFVQVVGGQFLDPNAAPAEKP
                     EESINARPVPLPNLPGSSVKPPSPAPAPSIPKAKKD"
     gene            complement(1674..2504)
                     /gene="ispE"
                     /locus_tag="VIS74_03255"
     CDS             complement(1674..2504)
                     /gene="ispE"
                     /locus_tag="VIS74_03255"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR00154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HEY5792288.1"
                     /translation="MLRVFASAKVNLWLHIADRRPDGFHEIETLMVPIGLRDEIQVER
                     AGTTGLLCSDPSVPDGEENLAIRAVRILEAETGRSLPVQITLQKNIPAGAGLAGGSSD
                     AVAVLKAVNSLYELNLDESRLIAAAARLGSDTAFFVRGAPALCRGRGEILAGAGDLRP
                     LRDRWLLLLKPPFGVPTPWAYSRWAALRPPAGESQSLGPFTLRNDLETPVFEKYTALP
                     VMKQWLLAQPGTEAALMSGSGSTLFAILPSEETGWELEKRARAFLGTPFWTCLTRFQI
                     "
     gene            2522..>3214
                     /gene="pyrF"
                     /locus_tag="VIS74_03260"
     CDS             2522..>3214
                     /gene="pyrF"
                     /locus_tag="VIS74_03260"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF001273.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEY5792289.1"
                     /translation="MKLPAKEKIITALDFDTADEARRLLDELAGEAAWVKIGLQLYSR
                     TGREVVDLALERGFRVFLDLKFHDIPNTVHEAIRSVATLGVHLTTIHASGGRAMMQEA
                     VKAAEGTNLLPLAVTVLTSLGAEDLHKAGILHTPGEQVAILAGLAKESGIRALVCSAQ
                     EAGMLRKKFGNHFTLVTPGIRRAADAAGDQKRVITPGDAIRNGADYLVVGRPITGAAS
                     PREAFLSIVDEIN"
BASE COUNT          616 a          973 c         1086 g          539 t
ORIGIN      
        1 tggatcgatg cgcacgaggt gaccatcggc caatacgcgg aattcctcga ctgggtgaaa
       61 aagaatccga accgtctcgt ggacatcgcc catccgaagg cgccgaaggg caaggattac
      121 attccggacg actgggcgga ccagacgcag atcgacatgc ccggctatta ctcgcgcgcg
      181 ctcaaatggg gcaaatacaa ggaggccgat ctggatctta attcgccggt tttcggggtg
      241 gatttcttcg acgcctgcgc ctacgcgaag tggaagggcc ggcgcctgcc gaccgagctg
      301 gaatgggaaa aagccgcccg cggaaccaag ggcaaccttt atccctgggg caacgagccg
      361 aacccgaaat gggtgaacag cggcgccgat ttcaaaccga atccgaagga aggcggcggc
      421 gtggacggct ggaaaaaatg gaacccggtg gacgccgtgg gcaaggacaa gagcgaattc
      481 ggggtcatgg atctggccgg caatgtgtcg gaatggacgg actcctgggt gcccagcgag
      541 gatcccgccc ttggcggcat gcaggtgccg gtcatccgcg gcggcaactg gcgcgcggag
      601 gattacaaac tcacccggcg cagcacggca ttgatggcgg aaaaacccga ttggcgggtg
      661 ggcttccgga ccgcttcgga gaaggctccg gacaagccgg cgaactaggc gtcttatgcg
      721 gttccggctt tttctcatcg catggcttgc cggcggcgcg gcgctgcacg cgcaggtgcg
      781 tgtcgaggtc agtgtgaatc gcggcctgta cattcggtat gagccaatcg ttgccacggt
      841 gagcctgacc aacctcagcg gccgggagtt ggagctttcg gacgacggca gccagccgtg
      901 gttcggtttt caaatccaaa cgtccgacgg acggcccgtt tccacccgca ccgccggtta
      961 tcaactcaac cccgcgcagg tcgggccggg acaatccctg acccggcaga tcaacctcac
     1021 cccgatttat gcgatggacc aatttggcgg ctaccgcatc cgggcatcgg tctttctgaa
     1081 aagcgaccgg caattttaca gctccccgcc gatcagcctg gaaatcaccg atggccggca
     1141 ggtctggcag caaaaggtgg gcgtcccggt gggccagccc gatgagggaa cgacccgcac
     1201 gatcacgctg ctcgcgcacc ggctgccgca aagcacggcg ttgtatatcc ggatcgagga
     1261 tccggaacgc ggccggattt tctgcacgca ccgcatcggg cgtttcgtga atttcggccc
     1321 gccgacggtg gagctggatt cgcagaatca ggttcacatc ctgcaattgc tcgcgccgcg
     1381 gaattatctg tattcgcact tcggattgaa tggcgaggtg ctggaccgca agctttacac
     1441 ctccaccggc accccgccgc ggatgcaacg gacgccggat ggttttgtgc aggtggtcgg
     1501 cggccaattc ctggatccta acgccgcccc ggcggagaaa cccgaggaat ccatcaatgc
     1561 ccgtccggtt ccgttgccca acctgcccgg cagctcggtg aagccgcctt cgccagcccc
     1621 ggcgccttcg attcccaagg caaagaagga ttgagttccg gcgcttgagc ccatcaaatc
     1681 tggaaccgcg tcaggcaggt ccagaagggg gttcccaaaa acgcgcgcgc gcgtttttcc
     1741 agctcccagc cggtttcctc ggaggggaga atggcgaaaa gcgtggaacc gctgccggac
     1801 atgagcgcgg cttccgtgcc gggctgggcg agcagccatt gcttcatcac gggcagcgcg
     1861 gtgtattttt cgaacacggg cgtttcgaga tcgtttcgca gggtgaaggg tccgagggac
     1921 tggctttccc cggcgggcgg acgcagcgcg gcccagcggg aatacgccca cggcgtgggc
     1981 acgccgaacg gcggcttgag gagcagcagc cagcggtccc ggagcgggcg gaggtcgccg
     2041 gcgccggcga ggatttcccc gcgtccgcgg cagagcgcgg gggcgccccg gacaaaaaag
     2101 gcggtgtccg aacccaggcg cgccgcggcg gcgatgaggc gggactcgtc cagattcaat
     2161 tcgtagaggc tgttgacggc cttgaggacc gccaccgcat cgctgctccc cccggcgagg
     2221 cccgcgccgg cgggaatgtt tttttggagc gtgatttgga cggggaggga gcggcccgtt
     2281 tccgcctcaa ggatccgcac cgcgcggatc gccagatttt cctccccgtc gggcacggag
     2341 ggatcgctgc aaaggagccc ggtcgtcccg gcccgttcca cctggatttc gtcgcgcagg
     2401 ccgatcggca ccatgagggt ttcgatctca tgaaatccgt ccggccggcg gtcggcgata
     2461 tggagccaaa gattaacctt ggcggatgcg aaaacccgaa gcatggatgg agttgaaaac
     2521 gatgaagctt ccagcaaaag aaaaaataat caccgcgctc gattttgaca ccgccgacga
     2581 ggcccggcgg ctgctcgatg agctggcggg cgaagcggcc tgggtgaaaa tcggcctgca
     2641 gctttattcc cgcaccgggc gcgaggtggt ggacctggcg ctggagcgcg ggttccgggt
     2701 attcctcgac ctgaaatttc acgatatccc gaataccgtg cacgaggcga tccggtccgt
     2761 ggccacgctg ggagttcacc tgaccacgat ccacgcttcc ggcgggcggg ccatgatgca
     2821 ggaggccgtg aaggcggcgg aggggacgaa tttattgccg ctggcggtga cggtgctgac
     2881 cagcctcggg gcggaggatt tgcacaaggc gggcatcctg cacacgcccg gcgagcaggt
     2941 ggcgatcctg gccggcctgg cgaaggagag cggcattcgc gcgctggttt gcagcgcgca
     3001 ggaggccgga atgctgcgca agaaattcgg gaaccatttc acgctcgtca ccccgggcat
     3061 tcgccgcgcc gcggacgccg cgggcgatca gaagcgggtg atcaccccgg gggatgcgat
     3121 tcgcaatggc gcggattatc tcgtggtggg ccgcccgatt accggagcgg cttcgccgcg
     3181 cgaagcgttc ctgagcatcg ttgacgagat caac
//
LOCUS       DATDAY010000167         2066 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_31367, whole genome shotgun sequence.
ACCESSION   DATDAY010000167 DATDAY010000000
VERSION     DATDAY010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2066)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2066
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_31367"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1309)
                     /gene="nadB"
                     /locus_tag="VIS74_03265"
     CDS             complement(<1..1309)
                     /gene="nadB"
                     /locus_tag="VIS74_03265"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006983247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HEY5792290.1"
                     /translation="MKAYDYLVIGSGIAGLTFALKAAAKGTVALVTKRRLVDSNTAWA
                     QGGVACVVSHEDSFELHIADTLTAGAGLCNEAAVRAIITGGPERVRELMELGMHFDER
                     ELENGGRELDLGREGGHSKRRVLHSHDTTGREIENTLVSRLREHPQIDVLENHMAVDF
                     LTTGKLGYAMENSCVGVYVLNEATGEVETLRSDVTVLASGGCGKVYLYTTNPDVATGD
                     GLAMAWRAGASIANMEFIQFHPTCLFHPEAKSFLISEAVRGEGAVLRNAKGRRFMEKY
                     HPRAELAPRDIVARAIDAEMKRTGEKCVWLDITHQPAEFIVSRFPTIYETCRKFGFDM
                     AAQPIPVVPAAHYQCGGVKTDLNGETSLRGLYAIGEVACTGLHGANRLASNSLLEGMV
                     MANRAFEKTARNRHPRVDFALPEWRPGQAGDVDEMVVIYHNWDE"
     gene            complement(1423..>2066)
                     /gene="panC"
                     /locus_tag="VIS74_03270"
     CDS             complement(1423..>2066)
                     /gene="panC"
                     /locus_tag="VIS74_03270"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HEY5792291.1"
                     /translation="FSRYPRTLAADSRICEREGANAILAFRKEEMYPAGYSTYVNEEA
                     LSGGLCGASRPGHFRGVTTVVAKLFNLVQPDIAVFGQKDWQQLAIVRRMVRDLNFPLR
                     IAGVPTVREADGLALSSRNQYLTPAERAEAPKIHAALGAARRRAKAGEKRAGILLRQT
                     RRELAKIAGGELDYLELVDAETLEPVSRVERTATLAVAFKFGGARLIDNVQLK"
BASE COUNT          355 a          709 c          614 g          388 t
ORIGIN      
        1 tttcgtccca gttgtggtag atcaccacca tttcatccac gtcgcccgcc tggccggggc
       61 gccactccgg cagggcaaaa tccacccgcg gatggcggtt gcgcgcggtc ttttcaaacg
      121 cccggttggc catgaccatg ccttcgagca aggaattgct cgccaggcgg ttggcgccgt
      181 gcaagccggt gcaggcgact tcgccgatgg catacagccc gcggagggac gtttcgccat
      241 tgagatcggt tttgacgccg ccgcattgat aatgcgcggc cggcacgacc ggaatcggct
      301 gcgccgccat gtcgaatccg aacttccggc aggtttcgta gatggtcggg aagcggctga
      361 cgatgaactc cgccggctgg tgggtgatgt cgagccagac gcatttttcg cccgtccgtt
      421 tcatctcggc atcaatcgcc cgcgccacga tgtcgcgcgg cgccagctcg gcccgcgggt
      481 ggtatttttc catgaaccgc cggcccttgg cattgcgcag cacggcgccc tcgccccgaa
      541 ccgcctcgct gatgagaaaa gacttggcct cgggatggaa caggcaggtc gggtggaact
      601 ggatgaactc catgttcgcg atgctcgccc ccgcccgcca cgccatggcg aggccgtcgc
      661 cggtggccac gtcgggattg gtcgtgtaga gatagacctt gccgcagccg ccgctggcca
      721 gcacggtcac gtcgctgcgc agcgtttcga cttcgccggt ggcttcgttc agcacgtaaa
      781 cgcccacgca ggagttctcc atggcgtagc ccaattttcc cgtcgtcagg aaatccaccg
      841 ccatgtgatt ttcgagcacg tcgatctgcg gatgctcccg cagccggctc acgagcgtgt
      901 tttcgatctc gcgcccggtt gtgtcgtgtg aatgcagcac gcggcgcttg gagtggccgc
      961 cctcccggcc gaggtcgagc tcgcgcccgc cattttccag ctcccgctca tcgaaatgca
     1021 tgccgagctc catcaactcc cgcacccgct ccggaccgcc ggtaatgatg gcgcgaaccg
     1081 ccgcctcatt gcacaggccc gctcccgccg tcagcgtgtc ggcgatgtgc agttcaaagg
     1141 aatcctcgtg gctgaccacg caggccaccc cgccctgcgc ccaggccgtg ttcgaatcca
     1201 ccaggcggcg tttggtcacc agcgccaccg tccccttcgc cgccgccttg agcgcaaacg
     1261 taagcccggc gatgccgctg ccgatgacga ggtagtcgta tgctttcatt tagccgcaag
     1321 gggatcttag acagaacgaa ccgaatttac aaaatgagga ctcaaccggc atcaccttaa
     1381 aattctgtcc attctgtcaa ttctgtcgaa acctttccct gcctatttca actgcacgtt
     1441 gtcaatcagc cgcgcgccgc cgaacttgaa ggcaaccgcg agcgtggcgg ttcgctcaac
     1501 gcgcgaaacc ggctccagcg tttccgcatc cacgagttcg aggtaatcca gctcgccgcc
     1561 ggcaatcttc gccagctccc ggcgcgtctg ccgcaataaa atccccgccc gcttttcccc
     1621 cgcctttgcc cgtcgccggg ccgcgcccag cgcggcgtgg atcttcggcg cctcggcgcg
     1681 ctcggcgggc gtcaggtatt gattgcgcga gctgagcgcg aggccgtcgg cttcgcgcac
     1741 ggtcggcacg ccggcgatgc gaaggggaaa attcagatcg cgcaccatgc ggcggacgat
     1801 cgccagctgc tgccagtcct tctggccgaa caccgcgatg tccggctgga cgagattgaa
     1861 taatttcgcc accaccgtgg tgacgccgcg gaaatgcccc ggccgggacg cgccgcacaa
     1921 tccgccggac agcgcctcct cgttgacata ggtcgaataa ccggcgggat acatctcctc
     1981 tttccggaag gccagaatgg cattcgcccc ctcgcgttcg cagatccgcg aatccgccgc
     2041 gagcgtgcgc ggataacgcg aaaaat
//
LOCUS       DATDAY010000168         2969 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_1267422, whole genome shotgun sequence.
ACCESSION   DATDAY010000168 DATDAY010000000
VERSION     DATDAY010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2969)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2969)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2969
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1267422"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..985)
                     /locus_tag="VIS74_03275"
     CDS             complement(<1..985)
                     /locus_tag="VIS74_03275"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY5792292.1"
                     /translation="MKVAFAFMSMPVGGAEDFALTISRQPRFAGETEFQFVCLRELGV
                     LGGEFAREGGRVECLPAAKSRRFSLPGVLRFARWLREQGIDLLHSQTYHAHTYAVPAA
                     WLAGIPVVLHQQKTLEKMKWRRALAFGMLARRADGMIALSKRTAEEIHRGFGIPHERL
                     FVLPNLVDETLFTPVDAAERRQLRVGLGLPPDRFLIGTVASLNAVKNHPATLAVAKGL
                     RDSGLEAAVWIFGEGKERSALEAAIAAEALGEHITLAGNRRPIHPWVQALDLFVLPSH
                     WEGQSLALLQAVACGIPVVASAIEGNLAVLGADHPGLFPAGGTAAYLQVCRH"
     gene            complement(999..1454)
                     /locus_tag="VIS74_03280"
     CDS             complement(999..1454)
                     /locus_tag="VIS74_03280"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEY5792293.1"
                     /translation="MKTILVPLDFSDLYPKVVDVAGQLARELDASLYLLQVVEPVTSY
                     VPVGAAMDVIVVPPPAPVAADLAEEKKTLEAVAAPLRADGLNVQTEVILGLPVDEILV
                     RAKTLPADFIVLGSHGHGALYHLFTGSVVTGVLKQAEIPVIVVPARRAA"
     gene            1520..2857
                     /locus_tag="VIS74_03285"
     CDS             1520..2857
                     /locus_tag="VIS74_03285"
                     /inference="COORDINATES: protein motif:HMM:NF023428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEY5792294.1"
                     /translation="MEDLFAAQEAERLQSAAPLAARMRPRTLADYIGQTHILGPGKLL
                     RRAIEADRISSILLYGPPGVGKTSLAQIIANSTHSRFERLSGVESTVADIRKTIANAA
                     LRLRNQDEKTILFIDEIHRFNKAQQDVLLPDVESGIVRLIGATTQNPFFYVNSALVSR
                     SQVFQLEPLSGADLLALMRQALADPERGLGTQKIELAPEAANHLAKLSDGDARKCLNS
                     LEIAAITTRPGGDGIIRIGLAEAEESIQRKAIVYEGDGDAHYDTISAFIKSMRGSDPD
                     AAIYWLAKMLHAGEDIRFIARRIVICASEDIGMADPQALVVAVAAQQACEFVGMPEAQ
                     IPLAQATVYLATAPKSNRSYAAIGAAMKDVASGRTLAVPKHLRDSHYQGAKQFGHGLD
                     YQYSHDFEGGYVPQAYLPEGRRYYEPTENGHEKRVKERLDYWRKLFEEAQKKP"
BASE COUNT          596 a         1015 c          929 g          429 t
ORIGIN      
        1 cgtggcggca gacttgcaaa tacgccgcgg tgccgcccgc cgggaacagc ccgggatgat
       61 ccgcgccgag caccgcgagg ttgccctcga tggcgctggc cacgacggga atgccgcacg
      121 ccaccgcctg gagcagggcc agcgactgcc cttcccaatg cgaaggcagc acgaacaaat
      181 ccagcgcctg aacccacgga tgaatcggcc gccggttgcc cgccagcgtg atgtgttcgc
      241 cgagcgcctc cgccgcgatc gcggcttcca gcgcggaacg ctccttcccc tccccgaaaa
      301 tccacaccgc cgcctccagc ccggagtccc gcaggccttt cgccacggcc agcgtcgccg
      361 gatggttctt cacggcattg agcgaggcca cagtcccgat gagaaaacga tccggcggca
      421 gccccagccc cacgcgaagc tggcggcgtt cggcggcgtc cacgggcgta aaaagggtct
      481 catccaccaa gttcggcagc acaaaaagcc gttcgtgggg aatgccgaat ccgcggtgaa
      541 tctcctccgc cgtgcgcttc gagagggcga tcatcccatc cgcccggcgc gcgagcattc
      601 cgaacgccag ggcgcggcgc catttcattt tctcgagcgt cttttgctgg tgcagcacga
      661 cgggaatccc cgccagccac gccgccggaa cggcgtaggt gtgggcgtgg taggtctgag
      721 agtgcagcaa atcaatgccc tgctcccgca gccagcgggc gaaccgcagc accccgggca
      781 gggaaaaacg cctggacttg gcggccggca ggcattccac gcggccgccc tcccgggcaa
      841 attccccgcc aagcacgccg agttcgcgca ggcagacaaa ttgaaactcc gtctccccgg
      901 cgaagcgcgg ctggcgggag atggtgaggg caaaatcctc cgcgccgccc accggcatgc
      961 tcatgaacgc aaacgcaacc ttcatttgga atcgtgaatc aagccgcccg ccgcgccggc
     1021 acgacgatca ccggaatttc ggcctgtttg agcacgcccg tgaccacgct gccggtaaag
     1081 agatggtaaa gcgcgccgtg cccatgcgag ccgagcacga tgaaatccgc cggcaacgtt
     1141 ttcgcgcgga ccagaatctc gtccaccggc aatcccagga tcacctcggt ctgaacgttc
     1201 aatccatccg cccgcagcgg agccgccacg gcttcgagcg ttttcttttc ttccgccaga
     1261 tcggcggcca ccggagccgg cggcggcacg acgatcacat ccatggccgc gcccaccggc
     1321 acgtaactgg tcaccggctc caccacctgc agcaaataaa gggaggcatc gagctcccgc
     1381 gccaattgac ccgcgacgtc caccaccttc ggatacaaat cgctgaaatc gagggggaca
     1441 agaatcgtct tcataaataa aggttcgcat acgcgactcg cgcttgcgag caaaagcgaa
     1501 cccgcggata ctgccgggaa tggaggattt attcgccgcc caggaagccg agcgcctgca
     1561 aagtgccgct cccctggccg cgcgcatgcg tccccggacc ctggcggact acatcgggca
     1621 gacgcacatc ctggggccgg gcaagctgct gcgccgggcc atcgaggccg accggatttc
     1681 ctcgatcctg ctctacgggc cgccgggcgt gggcaagacc agcctcgcgc agatcatcgc
     1741 gaattccacc cacagccggt tcgaacggct cagcggcgtg gagagcacgg tggcggacat
     1801 ccgcaagacc atcgcgaacg cggcgctgcg gctccgcaat caggatgaaa aaaccatctt
     1861 gttcatcgat gaaatccacc gcttcaacaa ggcgcagcag gacgtgctgc tgccggacgt
     1921 ggagagcggc atcgtccgcc tcatcggcgc gacgacgcag aatccgttct tttacgtcaa
     1981 cagcgcgctg gtctcgcgct cgcaggtctt ccagctcgaa ccgctctccg gggcggatct
     2041 cctcgcgctc atgcggcagg cgctggccga tccggaacgc gggctgggaa cgcaaaagat
     2101 cgaactcgcc ccggaggccg cgaaccatct ggcgaagctg agcgacggcg acgcgcgcaa
     2161 atgcctcaac tcgctggaga tcgccgccat taccacgcgg cccggcgggg atggcatcat
     2221 ccgcatcggg ctcgccgagg cggaggaaag catccagcgc aaggcgatcg tttacgaagg
     2281 cgacggcgac gcgcattacg acaccatcag cgccttcatc aaaagcatgc gcggttccga
     2341 tccggatgcg gccatttact ggctcgcgaa aatgctgcac gccggcgagg acatccgctt
     2401 catcgcgcgg cggatcgtga tctgcgcgag cgaggacatc ggcatggccg atccgcaggc
     2461 gctggtcgtg gccgtcgccg cccagcaggc gtgcgagttc gtgggcatgc ccgaggcgca
     2521 gattcccctc gcccaggcga cggtttacct cgccaccgcg ccgaagagca accggagtta
     2581 tgcggccatc ggcgcggcga tgaaggacgt cgcctccggc cgcacgctgg ccgtgccgaa
     2641 gcacctgcgc gacagccact accagggcgc gaagcagttc gggcacggat tggactatca
     2701 atacagccac gacttcgaag gcggctacgt gccgcaggct tatctgccgg aggggcggcg
     2761 gtattacgag ccgacggaaa atggccacga aaaacgcgtg aaggagcggc tggattactg
     2821 gcggaaactc ttcgaggaag cgcagaaaaa gccatgagga atttcgagga aagcctcgcg
     2881 gagctggacc gcgaatcgct ccgccgccgc tgccggacca gcacgccgct gccgcgggga
     2941 cggatcgaac gcgagggcaa aatctgtct
//
LOCUS       DATDAY010000169         2580 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_837410, whole genome shotgun sequence.
ACCESSION   DATDAY010000169 DATDAY010000000
VERSION     DATDAY010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2580)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2580)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2580
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_837410"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1674
                     /locus_tag="VIS74_03290"
     CDS             <1..1674
                     /locus_tag="VIS74_03290"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY5792295.1"
                     /translation="ISQLNDISARKQMERELREREEHFRRLADDTPAMLWMSGTELSS
                     HHFNKSALGFIGRNLEPEKGLGWLAAVHPDDRGDCLKACERGFAQRARFHLVYRLRRW
                     DGEYRIIQDISSPRYDNAGAFCGYIGVCTDITDQLNTETELRATLEIAREANQAKSTF
                     LAMMSHEIRTPLHSIIGFTHLLLDSGIPEDHRNLVSPILSGGEMLLAVINDILDYSKI
                     EAGKVELERLKFSLPQCVQEVSGLFSAKAGEKNLKFNHTMCSDVPELVVGDLNRLRQI
                     LTNLLSNAVKFTAHGHVSIAVSGEGFTENRHLLHFIIRDTGIGFPREKVADLFQPFRQ
                     LDTSTTRKYGGTGLGLAICKRLVDAMQGRIHVESSPNEGSTFHVHLPFEAADRQTPAL
                     PAARVHSPQNQALRPITLLSTKARALNILVAEDNETNRRLIAQLLNRMGLPSAQLVND
                     GHSALRACALQTFDLILMDCQMPVMDGYEATRRLRQTAAGKKTMVVALTAAALAGDKE
                     KALQAGMNDYLTKPIRPELLAEVLERAVPPGDDGAPLIRVIPPRPSRRE"
     gene            complement(1643..2122)
                     /locus_tag="VIS74_03295"
     CDS             complement(1643..2122)
                     /locus_tag="VIS74_03295"
                     /inference="COORDINATES: protein motif:HMM:NF020989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2017 family protein"
                     /protein_id="HEY5792296.1"
                     /translation="MAAPESGSVIVLGELPPHLVLMLQQIPIAEELSSADAEDRLFSE
                     PSTDPELVADWKAYVQPELLESFRTARQVVESDLKRMLEMEGRRSLEIPLSHADAWLN
                     ALNQLRLAIAAAHHLSDEELSREEAPQAQTTRELAILRIHFYGYLQHVILDAMGAGE"
     gene            complement(2125..2400)
                     /gene="clpS"
                     /locus_tag="VIS74_03300"
     CDS             complement(2125..2400)
                     /gene="clpS"
                     /locus_tag="VIS74_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006983651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HEY5792297.1"
                     /translation="MATETNEQAKTRNALGSVWQVVVHNDPVNLMSYVTLVLQRVFGY
                     PKEKAREHMLEVHQKGRSIVWSGEREKGELYVQQLHGFLLLATLERP"
     gene            complement(2401..>2580)
                     /locus_tag="VIS74_03305"
     CDS             complement(2401..>2580)
                     /locus_tag="VIS74_03305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792298.1"
                     /translation="IVHALRAAAFHVTGPAVAGSYLYQEAVGGLFPNHPGQVGACVEK
                     AMWMERAELPALRVA"
BASE COUNT          506 a          883 c          725 g          466 t
ORIGIN      
        1 atttcccagc tcaacgacat ctcggcccgc aagcagatgg aacgggaatt gcgggagcgc
       61 gaggaacatt tccgccggct ggccgatgac acccccgcca tgctctggat gtcaggcacg
      121 gaattgtcct cccatcattt caacaaaagc gcgctcggtt tcatcggccg caatctggag
      181 ccggaaaaag gcctcggctg gctggccgcg gttcatcccg atgaccgcgg cgattgcctg
      241 aaagcctgcg agcggggatt cgcccagcgc gcgcgtttcc acctcgttta ccgcctgcgg
      301 cgctgggacg gggaataccg gatcattcag gacatcagca gcccccgcta cgacaacgcc
      361 ggcgccttct gcggttacat cggcgtctgc acggacatca ccgatcagtt gaacaccgaa
      421 accgagctgc gggccacgct ggaaatcgcg cgggaggcga accaggcgaa aagcacgttc
      481 ctcgccatga tgagccatga aatccgcacg cccctgcaca gcatcatcgg cttcacccat
      541 ctcctgctcg acagcggcat cccggaggat caccggaacc tcgtcagccc gattctttcc
      601 ggcggcgaga tgctcctggc ggtcatcaac gacatcctgg actactcgaa gatcgaagcc
      661 ggcaaggtgg aactggagcg gctgaagttt tcgctgccgc aatgcgtcca ggaagtcagc
      721 ggattatttt ccgcgaaggc cggagaaaag aacctgaaat ttaatcacac gatgtgttcg
      781 gatgttcccg agctggtcgt gggcgatctg aaccggctcc gccagatcct gacgaacctg
      841 ctttccaacg ccgtcaagtt caccgcgcac ggccacgtgt ccatcgcggt ctccggagaa
      901 ggcttcacgg aaaaccggca tcttctccat tttatcattc gggacaccgg catcgggttc
      961 ccccgggaaa aagtcgccga tctctttcaa cccttccgcc agctcgacac ctcgaccacg
     1021 cgaaaatacg gcggcaccgg cctggggctg gccatctgca aacggctggt ggacgccatg
     1081 cagggccgca tccatgtcga aagctccccg aacgaaggct ccacttttca tgtccatctt
     1141 ccgttcgaag ccgcggaccg gcaaaccccc gcgcttcccg ccgcccgggt tcattccccg
     1201 caaaatcagg cgctgcggcc catcacgctt ctttccacca aagcccgcgc cttgaatatc
     1261 ctggtcgccg aggacaatga aaccaaccgc cgcctcatcg cccaactgct caaccgcatg
     1321 ggcctcccct cggcccagct ggtcaatgac gggcactccg cgctgcgggc ctgcgcgctg
     1381 caaacgttcg atctgattct catggattgc cagatgccgg tcatggacgg ctacgaggcc
     1441 acccggcggc tgcgccagac cgccgccggg aaaaaaacca tggtcgtcgc gctcaccgcc
     1501 gccgcgctgg cgggggacaa agaaaaggcc ctgcaggcgg gaatgaatga ttacctgacc
     1561 aaacccatcc gccccgaact cctggccgag gttctggaac gcgcggtccc ccctggggat
     1621 gacggagccc cgttaatccg cgtcattccc ccgcgcccat cgcgtcgaga atgacgtgct
     1681 gcaggtagcc gtaaaaatga atccgcagga tcgccagctc ccgcgtcgtt tgcgcctggg
     1741 gcgcctcctc gcggctgagt tcctcgtcgc tcaggtggtg ggccgcggcg atggccagcc
     1801 gaagctggtt cagggcgttt agccaggcgt cggcgtggct gagcgggatt tccagcgagc
     1861 gccgtccctc catttccagc atgcgtttca ggtcgctctc caccacctgc cgcgcggtgc
     1921 ggaaggattc cagcaattcc ggctgcacgt aagccttcca gtcggccacc agctcgggat
     1981 ccgtcgaagg ttccgaaaac aaccggtctt cggcatccgc ggaggaaagc tcttccgcga
     2041 tcggaatctg ctgcagcatc agcaccagat gcgggggcag ctcgccgaga acaatgaccg
     2101 atccggactc aggcgccgcc atttttaagg ccgctccaag gttgcgagca ggaggaaccc
     2161 atgcagttgc tgcacataca gctccccctt ctcccgttcg ccgctccaga caatggaacg
     2221 ccccttttgg tgcacctcca gcatgtgctc gcgggccttt tccttgggat acccaaacac
     2281 ccgctgcaga accagggtga cataactcat cagattcacc gggtcgttat ggaccaccac
     2341 ttgccagacg gagccaagcg cgttccgggt tttggcctgt tcgttggttt ccgtcgccat
     2401 ttatgccacg cgcagcgccg gcagctccgc ccgctccatc cacattgcct tttccacgca
     2461 cgcgcccacc tgcccgggat gatttgggaa caagccgccg acggcttcct gatacaaata
     2521 actgccggcc accgcgggtc cggtcacatg aaacgctgcc gcccgcagcg cgtgaacgat
//
LOCUS       DATDAY010000170         2438 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_834561, whole genome shotgun sequence.
ACCESSION   DATDAY010000170 DATDAY010000000
VERSION     DATDAY010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2438)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2438)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2438
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_834561"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..631
                     /locus_tag="VIS74_03310"
     CDS             <1..631
                     /locus_tag="VIS74_03310"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HEY5792299.1"
                     /translation="GGLVVAIAAMGSSMPAIAQPVKPGTGVVVPAGQPQPSPVPRKQK
                     PASELISKQDKPKVIPRVLEKAAPGNARIYISLGKQRAYLYVGDEIAVDTPISSGKRA
                     GMTPKGDYTVLEKDLDHRSSIYGNFVDRKGRVVRSGVSTKIDSAPSGTVFSGAPMKYF
                     LRLTWTGVGMHVGILPGYPASHGCVRMPEETAKLFFENVQTGVPATVGD"
     gene            complement(761..1297)
                     /locus_tag="VIS74_03315"
     CDS             complement(761..1297)
                     /locus_tag="VIS74_03315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792300.1"
                     /translation="MGRFLRLLPIIVLLASARAQEPEPAPPPPPPPPPAPAKESKPPG
                     PPTDYKERWEKLPPQEQERLSKHLTQHLKKWEDLSEAEKDRLRERGKQLFERGRKEVE
                     KAISQLDFELSPDKRREFIKTYMEKRREIERKLREETEPRRQQLIAEAIQALKKDFGG
                     KAAEPAPSATPAGAAGTP"
     gene            complement(1267..1605)
                     /locus_tag="VIS74_03320"
     CDS             complement(1267..1605)
                     /locus_tag="VIS74_03320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792301.1"
                     /translation="MDQTPKPPEENDALWELLGNARPPQASPFFASRVVHAARAESRE
                     RVPLLHFLLRPALALAAVAGLGFAAALFLEPRGSTQLASQDFEILSNLENLAALDTPT
                     QWDDSSAYFQ"
     gene            complement(1655..>2438)
                     /gene="rpoD"
                     /locus_tag="VIS74_03325"
     CDS             complement(1655..>2438)
                     /gene="rpoD"
                     /locus_tag="VIS74_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEY5792302.1"
                     /translation="YTKRHHELKDWHRKALRAKTEMVEANLRLVISIAKKYTNRGLSF
                     LDLIQEGNMGLMKAVEKFEYRRGYKFSTYATWWIRQAITRSIADQARTIRIPVHMIET
                     INKLIRVQKQLVQEYGREPNPEEVAEEIQLPVERVRAVLKMAQQPISLQAPVGDSEDT
                     SFGDFIEDKSAENPADQAAIVLLKDKIKDVLESLTERERQVLEQRFGLVDGYSRTLEE
                     VGRQFNVTRERIRQIEAKALRKMRHPTRIRQLEGFLEPQDLY"
BASE COUNT          384 a          729 c          815 g          510 t
ORIGIN      
        1 cggcgggctg gtggtggcaa tcgccgccat ggggtccagc atgccggcga ttgcccagcc
       61 tgtgaagccg ggaacgggcg tcgtggttcc ggcgggccag ccgcagcctt cccccgtgcc
      121 gcgcaagcaa aagccggcct ccgagttgat ctccaagcag gacaaaccga aggtgattcc
      181 ccgggtattg gagaaagccg cgcccggaaa tgcgcggatt tacatctcgc tgggcaagca
      241 gcgggcctac ctgtatgtcg gcgacgagat cgcggtggat acgcccattt cctccggcaa
      301 acgggcgggc atgacgccga agggtgacta cactgtgctc gaaaaggatt tggatcaccg
      361 ctcgagcatt tacgggaatt ttgtggatcg caaaggccgc gtggtgcgtt ccggggtgag
      421 cacgaagatt gattccgcgc ccagcggcac ggtcttcagc ggcgcgccga tgaagtattt
      481 cctgcggctg acctggacgg gcgtggggat gcacgtgggg attctgcccg gctaccctgc
      541 ctcccacggc tgcgtgcgca tgcccgagga aacggccaag cttttctttg aaaacgtcca
      601 gaccggcgtg ccggcgaccg ttggggatta gcgctttttc gggttaatcg gacgcgctct
      661 ccccggcggg gttggcaacc ccgccccacc acgaacccgt ctgccggtag ggcggcgttg
      721 ccaacgccgc cgttcatggc ggaactgaat cgaaaaagtc ttaaggtgta ccggcagcac
      781 ccgccggcgt ggcggagggc gccggttccg cggctttgcc gccgaagtct ttttttaatg
      841 cctgaatcgc ttccgcgatg agctgctggc ggcggggctc ggtttcctcg cgcagcttgc
      901 gctcaatttc ccgccgtttt tccatgtagg ttttgatgaa ttcccgccgc ttgtcggggg
      961 agagctcgaa gtccagctgg gaaatggcct tttccacctc cttgcggccg cgttcaaaca
     1021 attgcttgcc gcgttcccgc agccggtcct tctccgcctc gctcagatcc tcccatttct
     1081 tgaggtgctg ggtgaggtgc ttggaaaggc gctcctgttc ctgcggcggc agtttttccc
     1141 agcgctcctt gtaatccgtt ggcggcccgg gcggtttgct ttccttggcg ggcgccggcg
     1201 ggggaggggg cggcggcgga ggcgccggtt cgggttcctg cgcgcgggcc gaggccagca
     1261 ggacaattat tggaagtagg cggaggaatc gtcccattgc gttggcgtat ccaaggcggc
     1321 cagattctcg agattcgaga gaatttcaaa gtcctggctc gccagctgcg tggagccccg
     1381 gggttcgagg aacaaggcgg cggcgaatcc cagtccggcg acggcggcca gggccagggc
     1441 cggacgaagc aaaaagtgga ggaggggaac tcgttcccgc gattccgcgc gggccgcgtg
     1501 gacaacgcgg ctggcaaaaa acggcgaagc ctggggtggc cgggcgttcc ccagcagctc
     1561 ccagagcgca tcgttttctt ccggcggttt cggtgtctga tccacggtgg atttaactcc
     1621 ggtcggcgga ggaagtttcc gttttttccc cggatcaata caaatcctgc ggctcgagga
     1681 aaccctcaag ctggcggatg cgggtcgggt ggcgcatctt gcgcagcgcc ttggcttcga
     1741 tctggcggat gcgttcgcgc gtgacgttga actggcgccc gacttcctcc agcgtgcggc
     1801 tgtagccgtc cacaaggccg aaacgctgtt cgagcacctg gcgctcgcgt tcggtcagcg
     1861 actccagcac gtccttgatc ttgtccttga gcaggacgat cgccgcctgg tcggcgggat
     1921 tttccgcgct tttgtcctcg atgaaatcgc cgaagctcgt gtcctcgctg tcgcccacgg
     1981 gcgcctggag cgagatcggc tgctgggcca ttttcaacac ggcgcgcacg cgctcgacgg
     2041 gcagctggat ttcctcggcc acttcctccg gattcggctc gcggccgtat tcctgcacca
     2101 gctgcttctg cacgcggatg agcttgttga tcgtttcgat catgtgcacc gggatgcgga
     2161 tggtgcgggc ctggtcggca atggaacgcg tgatcgcctg gcggatccac cacgtggcgt
     2221 aggtcgagaa cttgtagccg cggcggtatt caaacttttc caccgccttc atgaggccca
     2281 tgttgccttc ctggatcagg tcgaggaacg aaaggccgcg attcgtgtat tttttggcga
     2341 tcgaaatgac caggcgcagg ttggcctcga ccatctcggt cttcgcgcgc agggccttgc
     2401 ggtgccagtc cttgagttcg tggtggcgct tcgtgtag
//
LOCUS       DATDAY010000171         3664 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_742245, whole genome shotgun sequence.
ACCESSION   DATDAY010000171 DATDAY010000000
VERSION     DATDAY010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 3664)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3664)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3664
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_742245"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2155
                     /locus_tag="VIS74_03330"
     CDS             <1..2155
                     /locus_tag="VIS74_03330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792303.1"
                     /translation="IGGFEDLGIPEYAEGVGSGLLNHPTGSVTLVNPTPDASTFAVSS
                     RPPNNRAYRGKLSGKAILPWLPAPRINEVTLCLRPRQAYASNADGSVGAAIPDQYYLE
                     MWVEVEVQTGAHDGKPANKQRMPISVVSHHVGLTFGLTHFDYKLANGAYNLQVDRYLK
                     PDHLRFLDGGASPPAGLTTLPIVSGEYKTIVSPANSHIIIPSSPHYTSNKEVQFHPTT
                     GNNPKVPIDKLHPRAWDPNGANPHHRPPTLSGQISVEIDMRLFLFGSTGTNVNAVYSM
                     VPVWDSQDTAPFAPPAGQADRLRWSFSLDLPAIGDGNKVTRSLEVIDNRLNGNSAAWD
                     VPSATPPTAEQLDENTLGQRNDRLAVFGTYDDSEISMHDFLQKSYEDVHRRSTIGALA
                     NVPIGMQRNRPFETLKFQPGGGGNPPDWLLLDLLSPSFRPFTFLHSATGKINPNARVA
                     PWSSGGDYTNDTVARWKPLQALLQGWNRSFNPVSASVTLPASGPSDLVKNILNHRFVG
                     AQYGAPDTYDYIGELCEVEGVADAGADNWEKEALIRNLANLLTTRSNSFTVHGVVQLV
                     KKRPGASNWAEWEPGDIITAQKRFHCGIERHVWPGVDGVPGNGTVQNGTLTKTAKPYG
                     MAAYDSSQPFIGYRPWCFVPVPKPAQGHYPIADAAFPAVDGPDAPNLWPVTGLQYLGA
                     NGWGKVPYTATSLEESANPARAWMRYRASDIVYDQ"
     gene            2109..2843
                     /locus_tag="VIS74_03335"
     CDS             2109..2843
                     /locus_tag="VIS74_03335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792304.1"
                     /translation="MPGCATAPATSFMINKFTAPLRPLLVLGILTLGWMPSGFGRAPV
                     IGMPAKDLLASLPAAPAQWEMKESWAKNDFSDWLTTIAQREFAGPVPLDPTTKLPAPK
                     LRPTVRFRITDTGYWPIRIFRYQNFEPGTSEGVERKLLKDSPAFVVAKSDESGTSIRI
                     SLLLRERFLVELTTKNLEMKDAEAQLDRVGFGKLRAVPANGETKLPQPMTLTCIDEMH
                     PQRTRTYQTFHMSLEEMEKARSIPPR"
     gene            2858..3550
                     /locus_tag="VIS74_03340"
     CDS             2858..3550
                     /locus_tag="VIS74_03340"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEY5792305.1"
                     /translation="MRGAIQLSRRWLRPRGFSLIELLTVIAVIAIIAVLIVPAFNRIG
                     QANRLSSAAFALMNTLSQARGEAIAQNTLVQLRVAATDVSGEQTAYRYYSLWRLDRDA
                     NGVESFQPLTRWEAIPQGVYLETDAAPMTKRAQKNSKYAARDYTGTHALNEPALNNRV
                     TGVPFRSGAVEAVYVEFLPDGSLNFPSLTTAAVYFMLSEGAPGSQASSTNPNWRAVFV
                     TALSGALAIREP"
BASE COUNT          757 a         1105 c         1059 g          743 t
ORIGIN      
        1 catcggcggt ttcgaagacc tcggcatccc ggaatatgcg gaaggagtcg ggagcggatt
       61 gctgaatcat ccgactggaa gtgtcaccct tgtcaatccg acgccggacg cttccacttt
      121 tgccgtatcg tcgaggcccc ccaataaccg ggcttatcgc ggaaagttga gcgggaaggc
      181 gatccttccc tggctcccgg cgccgcgcat caacgaagtg accctctgcc tgcggccacg
      241 ccaggcgtat gcatccaacg ctgacggatc agtgggggcg gccattccgg atcagtatta
      301 tttggagatg tgggtggagg tggaggttca gaccggcgcc cacgacggca agcccgcaaa
      361 taaacaacga atgcccatca gcgttgtctc gcaccatgtc ggactcacct tcggcctgac
      421 ccatttcgac tacaagctcg ccaacggagc ctacaatttg caagtggacc ggtatctgaa
      481 gccggaccac cttcgcttct tggatggggg cgcgtcaccc ccggcgggat taaccacgct
      541 tccgatcgtc tccggcgaat ataagacgat cgtttctccc gccaattccc atattatcat
      601 cccttcgagc ccgcattata cctcgaataa ggaggttcag tttcacccga cgacaggtaa
      661 taatcccaag gttcccatcg acaaactcca cccccgagcg tgggatccaa atggcgccaa
      721 cccgcatcat cggccgccga ccttgtcggg tcaaatctcc gtggaaatcg acatgcgttt
      781 gttccttttt ggaagcacgg gaaccaatgt caacgcggtt tattcgatgg ttcccgtctg
      841 ggattcgcag gacaccgcgc cgtttgcgcc tccggcaggc caggccgacc gcctgcgttg
      901 gagtttctcg ctggatctgc cggcgattgg cgacggcaac aaggtaaccc gcagcctcga
      961 ggtcatcgac aaccgcctca acggcaactc cgctgcctgg gatgtgccca gcgccacgcc
     1021 tcccaccgct gagcaattgg atgagaatac gctggggcag agaaatgacc ggcttgccgt
     1081 tttcggaacg tatgatgaca gcgaaatctc aatgcatgat ttccttcaaa aaagttacga
     1141 ggatgtccat aggcgatcca cgatcggggc gctggcgaac gttcccatcg ggatgcagcg
     1201 gaaccggcca ttcgagacgc tcaaattcca gccgggcggg ggggggaatc cgccggactg
     1261 gctgctgctc gatctccttt cgccctcgtt tcgtcccttt acctttctgc acagcgcaac
     1321 gggcaagatc aacccaaacg cgcgggtcgc gccgtggtct tccggcggcg actatacgaa
     1381 cgataccgtc gctcgatgga aaccgctgca agctttgctc caggggtgga atcgaagttt
     1441 taatccagtg agcgcttccg tcacgctccc ggcctcggga ccttccgatc tcgtaaaaaa
     1501 catcctgaac catcgcttcg tcggagcaca atacggcgcg cctgacacgt atgactacat
     1561 cggcgaactc tgcgaggtgg agggggtcgc ggatgcgggc gccgacaact gggaaaaaga
     1621 ggcgttgatc cgcaatctgg caaacctgct gactacccga agtaattcgt tcacggtgca
     1681 cggggtggtc cagcttgtaa aaaagcggcc cggcgcctct aactgggccg agtgggagcc
     1741 tggcgatatc attacggccc aaaaacgctt ccactgcggc atcgaacgtc acgtttggcc
     1801 cggggtggat ggggttcccg ggaacggaac cgttcagaac ggaacgctca caaaaactgc
     1861 aaaaccctac ggaatggctg cttatgacag cagccagcca ttcatcggtt accggccttg
     1921 gtgcttcgtt cccgtcccga aaccggctca gggccattac ccgatcgccg atgcggcgtt
     1981 tccggccgtg gatggccccg atgcgccaaa cctttggccg gttaccggcc tgcaatatct
     2041 gggggcaaac ggctggggta aggtgcctta tacggcgacc tcgctcgagg agtccgcaaa
     2101 ccccgcgcgt gcctggatgc gctaccgcgc cagcgacatc gtttatgatc aataagttca
     2161 ccgcgccgct gcgcccactg ctcgttttgg gaatactgac gctcggctgg atgccttcgg
     2221 gattcgggcg agccccggta atcggcatgc ccgcgaagga ccttctcgcc agcctccccg
     2281 ccgctccagc gcaatgggag atgaaggagt cttgggcaaa gaatgatttt tcagactggc
     2341 tgaccacgat cgcccaaagg gagtttgccg gcccggtccc gcttgatccg accacaaagc
     2401 tgccggcgcc aaaactgcgg cctacggtcc gttttcgaat cacggatacg ggctactggc
     2461 caatcaggat cttcaggtat cagaattttg agcccggaac aagcgagggc gtggagagga
     2521 agctgctcaa ggattcaccg gcctttgtgg ttgcgaaaag tgatgaatcc ggcaccagca
     2581 tccgcatcag tttgttgtta cgggagcgtt tccttgtgga attgacaacc aagaatctgg
     2641 agatgaagga cgccgaagcc caactcgatc gcgtcggttt cggcaaactt cgcgcggtgc
     2701 ccgcaaatgg agaaacgaag ctgccgcagc cgatgacgct gacatgcatc gacgagatgc
     2761 atccccaacg cacccgcacc tatcaaactt ttcatatgtc gctggaggaa atggagaaag
     2821 cgaggtccat tccaccgcgg tgactgtaaa aaatgagatg aggggcgcga ttcagctctc
     2881 ccgcagatgg cttcggccgc gcggattttc gctcatcgag cttttgaccg tgatcgcggt
     2941 gatcgcaatc atcgcggtat taatcgttcc cgcgtttaat cggatcggcc aggctaaccg
     3001 gctttcctcg gcggcgtttg cgttgatgaa cacgctatct caagcgcgcg gcgaagcgat
     3061 cgcccagaac acgctggttc agcttcgcgt ggcggcaacg gatgtgtccg gcgagcaaac
     3121 cgcttaccgt tactacagcc tttggcggct cgaccgggat gcgaacggcg tggagtcctt
     3181 tcagcctttg acccgatggg aggcgatccc ccagggcgtg tatttggaaa ccgacgctgc
     3241 tccgatgaca aaacgggcgc agaagaactc gaaatacgcc gcccgcgact acacgggcac
     3301 tcatgcgctc aacgagcccg ccttaaacaa tcgggtcacc ggcgttccct tccggtcagg
     3361 cgcggttgaa gccgtttacg tggaattttt gcccgatggt tcgttgaatt ttccttcgct
     3421 tacgaccgcc gcggtgtatt tcatgctctc ggagggagcc cccggctcgc aggccagttc
     3481 gactaatccg aactggcggg cggtttttgt cacggctctc tccggagcgt tggcgattcg
     3541 tgagccgtaa atgggagcgg tgggaacggc cggacaaatc gtttccctca ccgccgctgg
     3601 aagccttttc ggtttaattc gatgcgtttc cccggcgggg ttggctaccc cgcccccacc
     3661 gcga
//
LOCUS       DATDAY010000172         3914 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_1169432, whole genome shotgun sequence.
ACCESSION   DATDAY010000172 DATDAY010000000
VERSION     DATDAY010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 3914)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3914)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3914
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1169432"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1843)
                     /locus_tag="VIS74_03345"
     CDS             complement(<1..1843)
                     /locus_tag="VIS74_03345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792306.1"
                     /translation="MRCFLIIALCLAAAGSAARAQFGSFSDLPVEITSDGETRFEGGV
                     AIAENNVQIHYGDISIYTDYAEYNPDTRDVLLVGNIRLYSPEGMLTGQRSLFNLETRQ
                     MRSLEMQGEYFPLRFRAFNLRAPSLREFRVRDALMTTDDNSEPDFFVKSKTVRIYPNS
                     RVVFLNSTLYVKKLPIFWFPYIFANLDNTGFEFLPGYDSIWGAFLLTAYSFPIGSGNN
                     LTGKVHVDYRTERGFALGFDAKNAFGKDNRNYGELETYYAWDQDPGMGTNGNARPADI
                     TPDEGRYRVAYKQRLFLTDDIYGTAYINWLSDRFMLEDFFPNLFRTDPQPDTYVALTK
                     WDEFYSMNLLARWQINEFQQTTERRPEFSLDFKQHRLFGLPVYYDGENSFGSYKRAFE
                     DTSIFPDYEAVRFDTYHQFSAPVMLFNWLSLVPRAGMRFTYYSRTGSFKQVVDDPFNP
                     DVADSVESSPLNTPTSDLETGGGTFRPVFNTGLEASFKFSRTYERWQSRLLGLDGVRH
                     VVQPYANYSLVYNAGDSPDQILQFDRVVPSTQLTPITFPQFTAVDTIDTWNILRLGVR
                     NRIQTRRDNATFQYFSIDSFLDVNFENPYSDADVSNYFNIVNFNPVPW"
     gene            complement(1937..2911)
                     /gene="galE"
                     /locus_tag="VIS74_03350"
     CDS             complement(1937..2911)
                     /gene="galE"
                     /locus_tag="VIS74_03350"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEY5792307.1"
                     /translation="MKRIFVAGGAGYIGSICVEQLLNEGYEVAVFDNLSEGHRLALDS
                     RAIFFQGDLAERGSIDLALREFKPDAAMHFAANALVGESMQNPSKYFRNNVCNGLNLL
                     DGLLEHGVRKLVFSSTCATFGIPTRVPIDETLPQNPINPYGESKLLFEKILRWYDEIH
                     DLKFVALRYFNAAGATEKFGEDHRVETHLIPNILKVALGQKPAAQIFGTDYETPDGSC
                     IRDYIHVLDLASAHILALKHPQSDFFNLGTGGGTSVKEIIRVCREVTGREIPVVEQAR
                     RAGDPARLIAGSAKIRAALGWEPKYQGVQAIIESAWSWHVAHPNGYGD"
     gene            2981..3901
                     /locus_tag="VIS74_03355"
     CDS             2981..3901
                     /locus_tag="VIS74_03355"
                     /inference="COORDINATES: protein motif:HMM:NF014336.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1/P1 nuclease"
                     /protein_id="HEY5792308.1"
                     /translation="MKTEAAFFTVLLAAFSPLHAWDPAGHMLTTQIACGQLTPAAQAA
                     VEASIAAFDQKNQTSYTFVSAACWMDDIRGTQKSYAPWHYINLPFTPDADPKPGGGQP
                     PNVLWGIEYALGILKGQTADPNIDKDQALVILLHLIGDCHQPLHAASRPGDAGGNKVA
                     LANLKDPLLEIFPHYANLHFFWDSAYRRGFTEGFAVADYAPPFFPYNQALAGHAAALP
                     LVREKSRELLEKYPPESLDSGGAPADWVRESHQLGYDLGYQKLPGGETANPATLDAAY
                     VEAARECARKRLVQAGARIARTLNAIYPGP"
BASE COUNT          819 a         1156 c         1209 g          730 t
ORIGIN      
        1 gccaggggac cggattgaaa ttcacgatat tgaaatagtt cgagacgtcg gcatccgagt
       61 aaggattctc gaaattcacg tccaggaagc tgtcgatgga gaaatactgg aaagtcgcgt
      121 tatcccggcg cgtctgaatg cggttgcgca cgcccagccg gagaatgttc caggtatcaa
      181 tggtatccac ggccgtgaac tgcgggaagg tgatcggcgt cagctgggtg gagggaacca
      241 cgcggtcaaa ttgcaggatc tgatccggcg aatcccccgc gttgtaaacc agcgagtaat
      301 tcgcatacgg ctggacgaca tgccggacgc catccagccc gagcagccgg ctctgccacc
      361 gttcataggt gcgggaaaat ttaaacgacg cttcgaggcc cgtgttaaac accgggcgga
      421 aggtgccgcc gccggtctcg aggtccgaag tcggagtgtt cagcgggctg ctctccacgg
      481 agtcggccac atcgggattg aacggatcat cgaccacctg cttgaaggaa ccggtccggc
      541 tgtagtaggt gaaacgcatg cccgcccggg gcacgagcga gagccagttg aagagcatga
      601 cgggggcgga gaattgatgg taggtgtcga accgaaccgc ctcgtaatcc gggaagatgc
      661 tggtgtcctc aaacgcgcgc ttgtagctgc cgaaggagtt ttccccatcg taataaaccg
      721 gcaggccgaa gagccggtgc tgcttgaagt cgagggagaa ttccggccgg cgctcggtgg
      781 tttgctggaa ctcgttgatc tgccagcggg cgagcagatt catggaataa aattcatccc
      841 acttggtgag ggcgacgtag gtgtcgggct gcgggtccgt gcggaaaagg ttcgggaaga
      901 agtcctcgag catgaaacgg tcgctcagcc agttgatgta ggcggtgccg taaatgtcat
      961 ccgtcaggaa gaggcgctgc ttgtaagcga cccggtaacg gccttcgtcc ggagtgatgt
     1021 cggcggggcg ggcattgcca ttggttccca tgccgggatc ctggtcccag gcgtagtagg
     1081 tttccagctc gccgtagttg cgattgtcct tgccgaaggc gttcttggcg tcgaatccca
     1141 gcgcaaaacc gcgctcggtg cggtaatcca cgtgaacctt gccggtgagg ttgttgccgc
     1201 tgccgatcgg aaaactataa gcggtaagca ggaaggcgcc ccagatcgaa tcatacccgg
     1261 gcaggaactc gaagccggta ttgtcgaggt tcgcgaagat gtaggggaac caaaagatcg
     1321 ggagcttctt gacgtaaagc gtggagttca gaaaaaccac gcggctgttg ggataaatcc
     1381 ggaccgtctt ggacttcaca aaaaagtccg gttcggagtt gtcgtccgtg gtcatgagcg
     1441 cgtcccgcac ccggaactcg cgcagcgagg gcgcccgcag gttgaacgcg cgaaagcgga
     1501 gcgggaaata ttccccctgc atttcgagcg agcgcatctg gcgggtttcc aggttaaaaa
     1561 gcgagcgctg gccggtgagc atgccttccg gcgagtagag gcggatgttt cccacgagca
     1621 gcacatcccg ggtgtcggga ttgtattccg cgtagtccgt gtagatgctg atgtccccgt
     1681 agtggatctg gacattgttt tccgcaatgg ccaccccgcc ctcgaaacgg gtttccccat
     1741 ccgaggtgat ctccaccggg agatcgctga agctcccaaa ctgcgcccgc gcggcggaac
     1801 cagccgcggc aaggcaaagg gcgataatca ggaaacaacg catgggcaga ggcgcagagt
     1861 aatgggcgag tttcgcaatt caatgcgaaa atggcagcta aaaaacaccg ccgccctcat
     1921 tccggcattc gggtggctag tccccataac cgttcggatg ggcgacatgc cagctccagg
     1981 cgctttcaat aatggcctgc acgccctggt atttcggctc ccagccgagg gcggcgcgga
     2041 tcttggcgga gccggcgatc aaccgggcgg gatcccccgc gcggcgggcc tgttcgacga
     2101 cgggaatctc ccggccggtc acttcgcggc agacgcgaat gatttccttc accgaggttc
     2161 ccccgccggt tccgagatta aagaagtcgc tctggggatg cttcagcgcc agaatgtgcg
     2221 cgctggccag atccaggacg tggatgtagt cgcggatgca gctgccgtcc ggcgtctcgt
     2281 aatcggttcc aaaaatctgg gcggcgggtt tctggccgag cgccactttg aggatgttgg
     2341 gaatcaggtg cgtttccacg cgatgatcct ccccgaattt ttcggtcgct cccgccgcgt
     2401 tgaaataacg cagggccaca aatttcagat cgtgaatctc atcataccac cgcaggattt
     2461 tttcaaacaa cagcttggat tcgccgtagg gattgatggg attctgcggc agcgtttcgt
     2521 cgatcggcac gcgcgtgggg atgccgaacg tcgcgcaggt ggaggaaaac accagctttc
     2581 tcacgccgtg ttccagcagg ccgtcgagca ggttgaggcc attgcagaca ttgttgcgaa
     2641 aatatttcga gggattctgc atggactcgc cgaccagcgc gttcgcggca aagtgcatgg
     2701 cggcgtccgg cttgaattcg cgcagggcca gatcaatcga accgcgctcg gcgagatcgc
     2761 cctggaaaaa aatcgcccgg ctgtcgaggg ccaggcggtg gccttcgctg aggttgtcaa
     2821 aaaccgccac ttcgtaccct tcgttgagca gctgctcgac acaaatgctg ccgatatagc
     2881 ccgctccacc cgctacaaaa attcgtttca tgcgggcgca tcgtaaacag cccgtttcga
     2941 gcttggcaat agcaagaatg aggaatacca actcggctcc gtgaaaaccg aagctgcttt
     3001 cttcacggtt ctgctggccg ctttctcgcc gctccacgcc tgggatccgg ccggccatat
     3061 gctgaccacc cagatcgcct gcggccagct caccccggcc gcccaggcgg cggtggaggc
     3121 aagcattgcc gcattcgatc aaaaaaacca aaccagctac accttcgtga gcgccgcctg
     3181 ctggatggac gacatccgcg gcacccagaa gtcctacgcg ccctggcatt acatcaatct
     3241 gcccttcacg ccggacgcgg accccaagcc gggcggcggc cagccgccca atgtcctgtg
     3301 gggaatcgaa tacgccctcg gcatcctgaa ggggcagacc gcggatccga atatcgacaa
     3361 ggatcaggcg ctcgtcattc tgcttcacct catcggcgac tgccatcaac ccctgcacgc
     3421 cgccagccgc ccgggcgacg ccggcggaaa caaggtggcc ctcgccaatc tcaaggatcc
     3481 gctcctggaa atctttccgc actacgcgaa cctgcatttc ttctgggaca gcgcctaccg
     3541 ccgcggcttc acggagggct ttgcggtcgc ggattacgca cccccatttt tcccctacaa
     3601 ccaggctctg gccggtcacg ccgccgccct gcctttggtc cgggaaaaaa gccgggagct
     3661 gctcgaaaaa tacccgcccg aatcgctcga cagcggcggc gctccggcgg actgggtgag
     3721 ggaaagccac caactcggct acgacctcgg ttaccaaaaa cttcccggcg gcgaaaccgc
     3781 caatcccgcc acgctggacg ccgcctacgt ggaggcggcc cgggaatgcg cccggaaacg
     3841 cctcgtgcag gcaggcgcgc gcattgcccg gacgctgaac gcgatttatc ccggacccta
     3901 aaaaagtgga gggg
//
LOCUS       DATDAY010000173         2198 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_1317487, whole genome shotgun sequence.
ACCESSION   DATDAY010000173 DATDAY010000000
VERSION     DATDAY010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2198)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2198)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2198
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1317487"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..474)
                     /locus_tag="VIS74_03360"
     CDS             complement(<1..474)
                     /locus_tag="VIS74_03360"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY5792309.1"
                     /translation="MPKTILKGSVVLIGGGGKNLGGLLSRTFAAEGAKVALHFNSESS
                     RAEAEKTVADITAAGGEALAFQGDFTKPPEIAAFFAAAKAHFGGIDIAINTVGKVLKK
                     PFLETTEEEYDEMAAVNAKAAYFFIQEAGRHLNDGGKILTVVTSLLAAYTGLYSTY"
     gene            complement(563..1420)
                     /locus_tag="VIS74_03365"
     CDS             complement(563..1420)
                     /locus_tag="VIS74_03365"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEY5792310.1"
                     /translation="MNGTAKSLTKPYLRRVSGSGAAIEGLVLERVIEGGNAGDLHPPD
                     GPSKHVLVIPTSSARLDWKADGRGRAAWFQPGDILINPRGNFVAPRWSREVEFFLLGI
                     DPEFLAGCPDEWAVPPAGIDPRFHFRDELLFHLVRALASEFEGPAPADRLYTDALAGA
                     LMAHLLKGVLRRSAVSPKGGLSARQLAQVRDFIEAHLAGEIRLPALARQANLSPSHFL
                     ALFRRSTGLSPHQYLMRRRVERARSLLRQRNLSLVEIAQCCGFADQSHLTRMVRRYTG
                     ATPRQLREG"
     gene            1558..1857
                     /locus_tag="VIS74_03370"
     CDS             1558..1857
                     /locus_tag="VIS74_03370"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HEY5792311.1"
                     /translation="MSKIRVIARAVAQPGQETKVRELLQGMLAPTRAEAGCEFYELFE
                     SHEPGTFYFYELWESRAALDRHAATPHFLSMVAGVKDRLAAPFEVSLVRNLGEES"
     gene            1854..>2198
                     /locus_tag="VIS74_03375"
     CDS             1854..>2198
                     /locus_tag="VIS74_03375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792312.1"
                     /translation="MKIVRLFLAGLAGSAVFLRAAELSEVAQSPKYAWSGVAVSKTGR
                     IFVEFPRFQDLDGNPSVAEIRPDGTLRPFPGGAWNEWAPGKPAGNAFVSTNALHLFES
                     DPDSLWVIDTAAP"
BASE COUNT          403 a          696 c          700 g          399 t
ORIGIN      
        1 ataggtggaa tagagcccgg tgtaagccgc gagaagcgag gtgacgacgg tgaggatttt
       61 cccgccgtcg ttgagatgcc ggccggcttc ctgaatgaaa aaataggcgg ccttggcgtt
      121 gacggcggcc atttcgtcgt attcctcctc ggtggtttcg aggaagggtt tctttaaaac
      181 cttccccacg gtgttgatcg cgatatcgat gcctccgaag tgcgccttgg cggcggcgaa
      241 gaaggccgcg atctccggag gcttggtgaa gtcgccctgg aacgcgagcg cctccccgcc
      301 ggccgcggtg atgtccgcca cggtcttttc cgcctcggcc cgcgaggatt cactgttgaa
      361 gtgcagcgcg accttggctc cctccgccgc gaacgtgcgg ctgaggagtc cgccgagatt
      421 ttttcctccc ccgccgatga ggacgacgga acctttcaag atggtcttgg gcatagaatg
      481 ggaattgttt caaccaagct aaacgcggcc ggcgcggccg tcttgaacaa agccccgatt
      541 tttttgaaga aaatgccgga cttcagcctt cgcggagctg gcggggtgtg gctcccgtgt
      601 atcgccgcac catccgcgtc aggtggcttt gatcggcaaa tccgcagcac tgggcgattt
      661 cgacgaggga gagattccgc tgcctgagca ggcttctggc ccgctccacg cggcggcgca
      721 tcagatattg atgcggcgac aggcccgtgg accttcgaaa gagggccagg aaatgggagg
      781 ggctcaggtt cgcctgccgg gcgagcgccg gaagccggat ttccccggca agatgcgcct
      841 cgatgaaatc gcggacctgc gcgagctgcc gggcggacaa cccgcccttg ggggaaacgg
      901 cggaccggcg caagactccc ttcagcaggt gcgccatgag cgctccggcg agagcgtccg
      961 tgtataaccg gtccgccgga gcaggccctt cgaattccga ggccagcgcc cgcacgaggt
     1021 ggaacaacaa ctcgtcgcga aaatgaaacc gtggatcgat cccggccggc ggaaccgccc
     1081 actcgtcagg gcatcccgcg aggaattccg gatcgatgcc gaggaggaaa aactcgactt
     1141 cccggctcca gcgcggagcc acgaagtttc cccggggatt tatcagaatg tcgccgggct
     1201 ggaaccaggc cgcccgcccg cggccatccg ctttccagtc cagccgggcg gaagacgtcg
     1261 gaatgaccag cacgtgtttc gacgggccgt ccggcggatg aagatcgccg gcgttcccac
     1321 cttcgatcac ccgctccagg accagtccct cgatggccgc cccgctgcct gacacccgcc
     1381 tcagatacgg tttggtcagc gactttgcag tcccattcat ggctcgtctt ttagcatcgg
     1441 gcccgccgga aagcaaaaaa taacgcggcg ggccgccgcc gcaaaatccc cgatttgccc
     1501 ggcaaaacac cgcgcccggc gctttctccc gcgcggatct gggaaaatat tggaaatatg
     1561 agcaaaatcc gagtcatcgc ccgggccgtg gcccagcccg gccaggaaac gaaagtgcgc
     1621 gagctgcttc agggcatgct cgcccccacc cgggcggaag cgggctgcga gttctacgaa
     1681 ctcttcgagt cgcacgaacc ggggacgttt tatttttatg aattgtggga gagccgggcc
     1741 gcgctggacc ggcacgccgc cacgccgcat tttttgagca tggtggcggg cgtgaaagac
     1801 cggctggcgg ctcccttcga ggtcagcttg gtccgcaacc tcggggagga atcatgaaaa
     1861 tcgtccggct gtttctcgcc gggctggcgg gttcggcggt tttcctccgc gccgcggaac
     1921 tctccgaagt cgcgcaatcc ccgaaatacg cctggagcgg tgtggccgtc tcgaaaaccg
     1981 gccgcatctt cgtggagttt ccccgctttc aggatttgga tggcaatccc tcggtcgccg
     2041 aaatccggcc cgatggcacg ctccggccat ttcccggcgg cgcgtggaac gaatgggctc
     2101 ccggcaagcc ggccggcaac gccttcgtga gcacaaacgc gctgcacctt tttgagagcg
     2161 atcccgactc gctgtgggtc attgataccg ccgcgccc
//
LOCUS       DATDAY010000174         2295 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_942787, whole genome shotgun sequence.
ACCESSION   DATDAY010000174 DATDAY010000000
VERSION     DATDAY010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2295)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2295)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2295
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_942787"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..652
                     /locus_tag="VIS74_03380"
     CDS             <1..652
                     /locus_tag="VIS74_03380"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HEY5792313.1"
                     /translation="RAVKLTQETIKLAALIGARAVILHGGRIRALPLHRRLMRLVREK
                     KFLTKEYAREKLRAVLQREKAGPLYLERLVSALTPLLALAAENGVHLGLENRERYEDV
                     PSEREILPLLARFNSPYLGYWHDFGHAQIKHNLALLDHADWLRLAGNRIFGGHLHDVT
                     FPNLDHLPPFTGELDYAQLIPLLPAGCPLILEMHPDTPAGQIQQAAAEWRRRFGTS"
     gene            649..1674
                     /locus_tag="VIS74_03385"
     CDS             649..1674
                     /locus_tag="VIS74_03385"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HEY5792314.1"
                     /translation="MKRLITTSLQIALTAALLYWVLHDPAKRAAMADSLRAAKPEWLA
                     AALVCIGLGFGLQIVRWNWLLKTLDIALPPLRVVRLYFVGLFFNLFIPGGTGGDLVKI
                     YYTAREKPDQKYGAAMSVILDRVFGMLALILIGGVIGTLFFHALMATPITRGMLFMVA
                     GIFAASLAVVILAWIVEAFQLAGRIPAWMPLRHSILELAAGFSAVARRPRTVLACIGI
                     SLPAHGLLFLCFYCVARALTDQLSLAAIFSVLPITQTIASLPISFGGLGVREEAFQQL
                     LGTLYHTPIDKAALISLGGYSMALIWGLAGGAVYLTYRTSQGGKVSLHAARQEAETLE
                     HDLEKSP"
     gene            1671..>2295
                     /locus_tag="VIS74_03390"
     CDS             1671..>2295
                     /locus_tag="VIS74_03390"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RibD family protein"
                     /protein_id="HEY5792315.1"
                     /translation="MRETGRPFVIANFAMSADGKISTRNRTPALFTSAHDKARLLKIR
                     AMGDAVIAGRHTVETDTMSLGIPGRPPEKCPLRVLISESGKISPKWKVFHSPGGAIVI
                     FTTPAISREARTALEAHPQATLHLAKTVRLPRVLKILRQQHGVRKLVCEGGPRLFRSL
                     LEIGALDQLRVTIAPRLFGGAAAPTLTGVSTHFLRSIPAFRLTRCEVI"
BASE COUNT          385 a          795 c          669 g          446 t
ORIGIN      
        1 ccgcgcggtc aagctgacgc aggaaaccat caaactggcc gccctcattg gcgcgcgcgc
       61 ggtcatcctt cacggcggac gaatccgggc gctgcccctg caccgccggc tcatgcggct
      121 ggtgcgcgaa aaaaaatttc tcaccaagga atacgcccgc gagaaactcc gcgcggtcct
      181 ccagcgcgaa aaggcggggc cgctttatct cgaacgcctg gtctccgccc tcacgccgct
      241 cctggccctg gcggcggaaa atggggtgca tctgggattg gaaaatcgcg aacgctacga
      301 ggacgttccc tcggaacggg aaatcctccc gctgctcgcg cgctttaatt cgccgtatct
      361 gggctactgg cacgacttcg ggcacgccca gatcaaacac aacctcgcgc tgctcgatca
      421 cgccgactgg ctgcggctgg ccggcaaccg cattttcggc ggtcatctgc acgatgtcac
      481 ctttcccaac ctcgatcatc tgccgccctt cacgggcgag ctggactatg ctcaactgat
      541 cccgctcctg ccggcgggct gccccttgat ccttgagatg catcccgaca cgccggcggg
      601 acagattcaa caggcggccg cggaatggcg ccgccgcttc ggaacttcat gaaacgcctc
      661 atcaccacct ccctccaaat cgccctcacg gcagccctgc tctattgggt gctccacgat
      721 cccgccaaac gagccgcgat ggcggattca ctccgcgccg cgaaaccgga atggctggcc
      781 gccgcgcttg tctgcatcgg cctcggcttc gggcttcaga tcgttcgctg gaactggctc
      841 ctgaaaactt tggacatcgc gctgcccccg ctgcgggtgg tgcggctgta ttttgtcggc
      901 ttgttcttca acctgttcat tcccggcggc acgggcggcg atctggtgaa aatttattac
      961 acggcccggg aaaaaccgga tcaaaaatac ggcgccgcca tgagcgtcat cctcgaccgc
     1021 gtgttcggca tgctcgcgct catcctgatc ggcggcgtga tcggcacgct cttcttccac
     1081 gcgctcatgg ccacgcccat cacccggggc atgctgttca tggtcgccgg catttttgcc
     1141 gcttcgctgg cggtggtgat tttagcctgg atcgtggaag ccttccagct ggccgggcgc
     1201 attccggcct ggatgccgct gcgccattcc atcctggaac tggcggccgg attttccgcg
     1261 gtggcccggc ggccccgcac ggtgctcgcc tgcatcggca tcagcctgcc ggcgcacggc
     1321 ctgctgttcc tctgttttta ttgcgtcgcc cgcgcgctga ccgatcaact cagcctggcc
     1381 gcgatttttt cggtgctccc gatcacccaa accatcgcct ccctgccaat cagcttcggc
     1441 gggctgggcg ttcgcgagga ggccttccaa caactgctcg gcacgcttta tcacaccccc
     1501 atcgacaagg ccgcattgat cagcctcggc ggttatagca tggccctgat ctggggactc
     1561 gccggcggcg cggtttatct cacgtatcgc acctcgcagg gcggcaaggt ttcgctgcac
     1621 gccgctcggc aggaggcgga aacccttgag cacgatctcg aaaaatcccc atgagggaaa
     1681 ccgggcggcc tttcgtgatc gcgaatttcg cgatgagcgc ggatggaaag atctccaccc
     1741 gcaatcgcac tccggcgtta ttcacctcgg cgcacgacaa ggcccgcctg ctgaaaatcc
     1801 gggcgatggg cgatgcggtc atcgctggcc gccacaccgt ggagacggac acgatgagcc
     1861 tcggcatccc cggccgcccg cccgaaaaat gcccgctacg cgtgctcatt tccgagtcgg
     1921 gaaaaatttc cccgaaatgg aaggtgtttc actcccccgg cggcgcgatc gtgattttca
     1981 ccaccccggc gatttcccgc gaagcgcgca cggccttgga agcccatccg caggcaaccc
     2041 tgcatctggc gaaaacggtc cggctcccgc gggtgctgaa aattctgcgc cagcaacacg
     2101 gggtccgcaa gctggtgtgc gaaggcggcc cgcgactctt ccgctccctt ttggaaatcg
     2161 gggcgctgga tcaattgcgg gtgacgattg cgccgcggtt gttcggcggc gccgccgcgc
     2221 ccacgctcac gggggtatcc acccattttc ttcgatccat cccggcgttt cgcctcacgc
     2281 gctgcgaggt gatcg
//
LOCUS       DATDAY010000175         2247 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_2046566, whole genome shotgun sequence.
ACCESSION   DATDAY010000175 DATDAY010000000
VERSION     DATDAY010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2247)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2247)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2247
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2046566"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1333)
                     /locus_tag="VIS74_03395"
     CDS             complement(<1..1333)
                     /locus_tag="VIS74_03395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEY5792316.1"
                     /translation="MAGSPAKGGSVSADPRVDLHVHSRHSTRSADWLLRRFDFPSSYT
                     DPLTLLARLREAGMRFVTITDHNTLAGCLEIAHLPDVFLSEEVTTHFPEDGSIVHLLV
                     WGITESQHKDIQKLRENIYDLRGYLAGNGVAHAVAHLFHSPDEKLHAANLQKLILLFK
                     HFEVVNGRYDGILSQSARHALEKLTPRRIEQFVARLGIEPTHEEPWKKIFVGGSDDHG
                     GVFPARAFTRTPPAASVPEFLDHLRAGRCLAEGRNGTPLTLAHSTYSTAFQFIRGKMS
                     ANQEAPGTALIEKVFARFMEGKDPTEFSFAEKFGFLMQGLATGKIFELAKAGNSSLWK
                     EMSAYFSRPEVKAALARETEGVKEPERRAFLMANLIANQLGFRFFSQFVAQITGGNLL
                     ESIQMISPLVPIAGILTPYFRAFSMPKRRTLRELSQAACGEIVPELRNVRRA"
     gene            complement(1300..2082)
                     /locus_tag="VIS74_03400"
     CDS             complement(1300..2082)
                     /locus_tag="VIS74_03400"
                     /inference="COORDINATES: protein motif:HMM:NF021601.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEY5792317.1"
                     /translation="MQNAPDPADPTVSGLVVSIHDVSPATREMVELMIADLRAAGVAK
                     TSLLVIPNHHHRGVSLEDPAFAGWLAELERQGHEVVVHGYYHRREAKAKEGAFTKWVT
                     RHYTAGEGEFFDLPESEAQPLLDRALAEFRAGGFAPKGFIAPAWLLGWEAELAVRSRG
                     FDYTTRIASLTDLRRNLVYHSRSLVYSARAFWRRQVSLLWNWFVLHRVRYWPLVRMGL
                     HPPDWKYPLIRAHALGCIRHAMKKRPPMTYWEWLEAQRKAGA"
BASE COUNT          423 a          721 c          700 g          403 t
ORIGIN      
        1 aagcccgccg aacattgcgc aactcgggca cgatctcgcc gcaggccgct tggctgagtt
       61 cgcgcagcgt ccggcgcttc ggcatggaaa aggcgcgaaa atacggcgtg aggatgccgg
      121 cgattggcac cagcgggctg atcatctgga tgctctcgag caaattgcct cccgtgatct
      181 gcgccacgaa ctgggagaaa aaccggaaac cgagctggtt ggcgatgagg ttcgccatca
      241 aaaaggcgcg ccgctcgggt tccttcaccc cctcggtttc gcgcgccagc gcggccttga
      301 cctcgggccg ggaaaaatag gcggacatct ccttccacag cgagctgttg ccggccttcg
      361 cgagctcgaa gatcttgccg gtggccagcc cctgcatgag aaatccaaac ttctccgcga
      421 agctgaactc cgtgggatcc ttgccctcca tgaaacgcgc gaacaccttc tcgatcagcg
      481 cggttccggg cgcctcctga ttggcggaca ttttcccccg gatgaactgg aacgccgtgc
      541 tgtaggtgct gtgcgcgaga gtcagcggcg tgccgttgcg cccctcggcc aggcagcggc
      601 cggcgcgcag gtgatcgaga aattccggca cggatgccgc cggcggcgtg cgggtgaacg
      661 cccgggcggg aaagacgccg ccgtggtcgt ccgaaccgcc cacgaagatt tttttccagg
      721 gttcctcgtg cgtgggctcg atgccgaggc gcgcgacaaa ttgctcgatg cgccgcggcg
      781 tgagcttttc caaggcatgc cgcgcgctct ggctcagaat cccgtcgtag cggccgttga
      841 ccacctcgaa gtgcttgaag agcaggatga gcttctgcag gttcgccgcg tggagctttt
      901 catccgggct gtggaacagg tgcgccacgg cgtgtgcgac gccattgccc gcgaggtagc
      961 cgcggagatc gtagatattt tcccgcagct tctggatgtc cttgtgctgg gattcggtga
     1021 tgccccacac gagcaggtgg acgatgctgc catcctccgg aaaatgggtg gtcacctcct
     1081 cgctgaggaa cacgtccggc agatgggcga tctccaggca gccggcgagg gtgttatggt
     1141 cggtgatcgt cacgaaccgc atgcccgcct cccgcagccg ggcaagcagc gtcagcggat
     1201 ccgtgtagct ggagggaaaa tcaaaacgcc gcagcagcca gtccgccgaa cgcgtgctgt
     1261 gccggctgtg aacgtggaga tccacccgcg gatcggcgct cacgctcccg cctttcgctg
     1321 ggcttccagc cattcccaat acgtcatcgg cggccgtttt ttcatcgcgt ggcggatgca
     1381 gccaagggcg tgagcgcgaa tcagcggata cttccagtcc ggcgggtgca gtcccattcg
     1441 caccagcggc caataacgca cgcggtgcag cacaaaccag ttccacagca ggctcacctg
     1501 ccgccgccag aaggcccgcg cgctgtaaac caggctgcgg gaatgataca cgagattccg
     1561 ccgcaggtcg gtgagggaag cgatgcgcgt ggtgtagtca aatccccggc tgcgcaccgc
     1621 gagttcggcc tcccagccga ggagccaggc cggggcgatg aaccccttcg gcgcaaatcc
     1681 gcccgcccgg aattcggcca gcgcgcggtc cagcagcggc tgcgcctcgg attccggcag
     1741 atcgaaaaat tccccctcgc ccgcggtgta atggcgcgtc acccatttgg tgaacgcccc
     1801 ttccttcgcc ttggcttccc tccggtgata atacccatgg acgacaactt cgtgcccctg
     1861 ccgctccaac tccgccagcc agccggcgaa ggcgggatcc tccagcgaaa cgccccggtg
     1921 atggtggttc ggaatgacca gcagcgaggt cttcgccacc cccgcggcgc ggaggtcggc
     1981 aatcatcaac tccaccattt cccgcgtggc gggactgaca tcgtggatgg aaacgacgag
     2041 tccggacaca gttggatcgg cgggatcggg tgcattttgc atttgcgaat ctcggttgat
     2101 acgtgaatgt tactttctat ccaagtaccc gatgtctcag gaagcaccga atccatcaaa
     2161 agataaccgc cagcgcaacg gcaataacaa caatgagcca caaatgaact ggcgcggcct
     2221 cattctcctc atgctggcga tcggcct
//
LOCUS       DATDAY010000176         3400 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_1266766, whole genome shotgun sequence.
ACCESSION   DATDAY010000176 DATDAY010000000
VERSION     DATDAY010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 3400)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3400)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3400
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1266766"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1678)
                     /locus_tag="VIS74_03405"
     CDS             complement(<1..1678)
                     /locus_tag="VIS74_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEY5792318.1"
                     /translation="MSSYKDTLNLPKTAFPMKAGLTEREPRMLARWEAEGIYEKIQAA
                     RAGSPDFILHDGPPFANGDVHMGTALNKVLKDLVVKSKTMAGYRAPFVPGWDCHGLPI
                     EFKVVKESRGLSPVQIRQRSEEYARKYIDIQRRQFRRLGVFGDWENPYLTLDPGYEAE
                     IIRSFGRFVEKGLVYLGKKPVLWSTGAQTALAEAEVEYAKRTDPAIYVKFAVNSETAP
                     KLFEAGFLKREASFVIWTTTPWTLPANVAIALHPNEPYGVQTYRKDGAEFDLILAYRR
                     MEAFTAETGLEPVGAPVGTWPGEDFRLAVAEAMHPFLARHSKIITADFVTMETGTGQV
                     HIAPGHGKDDYLAGQQNDLPTLSPVDDHGRFTDEVGMPQWVGKYVFDANKEIVEFLRE
                     QGSLLAVHDYSHDYPHCWRSKTPIVFRAVEQFFIRVDDIRGPALNAVDQTKWLPQWGR
                     NRIAGTVESRPDWCISRQRTWGVPLPVFYDAAGGPVIDQSVIEKVADLVKERGTNLWF
                     EKDDAWWAEAVGLPAGTTRRNDTLDVWIDSGVSHEAVLRHRPELRFPADIY"
     gene            1781..2749
                     /locus_tag="VIS74_03410"
     CDS             1781..2749
                     /locus_tag="VIS74_03410"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY5792319.1"
                     /translation="MTPHTKGLLFLFLVTLAWGLSFCLVKALSLALGEGWGPAAGIIS
                     IRFLLAALPLIGLQLLKGRWRPTAAEWRQGGLIAFFCATGMLLQAHALSYTLASTSAF
                     LTQGCAILVPLWVALTRRQLPPAKILLPLALVIAGVAILSGVDFRTFRMGRGEFETLL
                     SCVFFTGLMLTIESPRYARTDKALVTVVFLLLGGLGALPLALANGGGESLAILLGQGS
                     TLLVMAVLAFISTTLGFYWMVKWQPNVTATEAGIVYCLEPVWASLLSLFLPVILAGLM
                     GVAYANEQITLAMVLGGGLILSASVLLALVTPPPKTAAPDLPKAGA"
     gene            2767..3192
                     /locus_tag="VIS74_03415"
     CDS             2767..3192
                     /locus_tag="VIS74_03415"
                     /EC_number="1.11.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0006972 - hyperosmotic response [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0033194 - response to hydroperoxide
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEY5792320.1"
                     /translation="MKRTGSAHWQGNLNDGKGTVSTPSGALKETPYSFKARFETENGT
                     NPEELIAAAHAGCFTMALSLFLGNAGFTPESLDTVAEVSLDQVKSAWAVTAIKLTLKA
                     KVPGIEEAKFQELAAFAKANCPISKLLDAPITLEASLAA"
     gene            complement(3189..>3400)
                     /locus_tag="VIS74_03420"
     CDS             complement(3189..>3400)
                     /locus_tag="VIS74_03420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792321.1"
                     /translation="QRFSGDEIYARFGQQDLTADVNFRDVRNWAKAAGFRREFDGTQG
                     EFFARHLGRAGGAETGHFRVLALGR"
BASE COUNT          622 a         1076 c         1023 g          679 t
ORIGIN      
        1 gatagatgtc cgcgggaaac cgcagttcgg gacggtgccg cagcaccgcc tcatgcgaga
       61 cgccggagtc gatccaaacg tcgagcgtgt cattccgccg cgtcgtgccg gcgggcaggc
      121 caaccgcctc cgcccaccag gcgtcgtcct tctcgaacca aagattcgtg ccccgctcct
      181 tcacgaggtc cgcgactttt tcaatgaccg attgatcgat gaccgggccc ccggccgcat
      241 cgtaaaaaac gggaagcggc acgccccagg tgcgctggcg ggaaatgcac cagtcgggcc
      301 tcgactcgac cgtgccggcg atgcgattgc gtccccattg cggcagccat ttagtttgat
      361 ccacggcgtt taatgccggc ccgcggatgt catccacgcg aatgaaaaat tgttcgaccg
      421 cccggaagac aatcggcgtc ttcgaccgcc aacaatgcgg gtaatcgtgg gagtaatcgt
      481 gaaccgccag caacgagcct tgttcgcgca ggaactcgac gatctccttg ttcgcgtcga
      541 agacgtattt cccaacccac tgcggcatcc cgacctcgtc ggtgaacctg ccgtggtcat
      601 cgacggggga aagggtcggc agatcgttct gctggccggc aaggtagtca tccttgccgt
      661 ggccgggcgc gatgtgaacc tggccggtgc cggtttccat ggtgacgaaa tccgcggtga
      721 taattttcga atggcgcgca agaaacggat gcatcgcttc agcaaccgcc aatcggaagt
      781 cttctcctgg ccaagttcca acaggtgcgc caactggttc aaggccggtt tcagcagtaa
      841 atgcctccat ccggcgataa gccagaatca agtcgaattc agctccatcc ttgcgataag
      901 tctgaacccc gtaaggttcg tttggatgaa gcgcgattgc cacgttggct ggaagtgtcc
      961 atggcgtggt tgtccaaatt acaaacgagg cctcgcgttt taaaaatccg gcttcgaaca
     1021 atttcggcgc ggtctctgag ttgaccgcaa acttcacata gatcgccgga tccgtgcgct
     1081 tcgcgtattc cacctcggct tcggccagcg cggtctgcgc gcccgtgctc caaagcacgg
     1141 gttttttacc cagataaacg agtccttttt cgacgaagcg gccgaaggag cggatgatct
     1201 cggcttcgta gccgggatcg agcgtgaggt agggattttc ccaatcgcca aagaccccaa
     1261 gccggcggaa ctggcggcgt tgaatgtcga tgtatttgcg cgcgtattcc tccgagcgct
     1321 ggcggatttg caccggcgaa agaccgcggg attccttgac caccttgaac tcaatcggca
     1381 ggccgtggca gtcccagccc ggcacgaagg gcgcgcggta tccggccatc gtcttcgact
     1441 tcaccaccag atccttgagc accttgttga gcgccgtgcc catgtggacg tcgccattcg
     1501 cgaagggcgg gccgtcgtgc aaaatgaaat ccggcgatcc cgcgcgggcg gcctggattt
     1561 tttcataaat gccttccgcc tcccagcggg ccagcatccg gggttcgcgt tcggtcaggc
     1621 ccgccttcat cggaaaggcg gttttgggca gattgagagt gtccttataa gaactcatga
     1681 taaacggaaa agggaaaaac atagcggggt cttcgcaact ttacagtttt aagttttccg
     1741 tggccggaag caccggggtg gcctacaggt tccgtccgcc atgactccgc acaccaaagg
     1801 tctcctgttc ctgtttctcg tcacgctcgc gtggggactg agcttctgcc tggtgaaggc
     1861 gctcagcctg gccctcggcg aaggctgggg accggccgcg ggaattatca gcatccgctt
     1921 cctgctggcg gcgcttcccc tgatcggctt gcagttgctc aagggccgct ggcggcccac
     1981 cgccgcggaa tggcggcagg gcgggctcat cgcctttttt tgcgccaccg gcatgctgct
     2041 gcaggcccac gccctgagtt acacgctcgc ctcgacgtcc gcgttcctga cccagggctg
     2101 cgcgatcctg gtgccgctct gggtggcgct cacgcgccgc caattgcctc cggccaaaat
     2161 cctgctgccg ctggcgctgg tcatcgccgg ggtggccatt ctttccgggg tggacttccg
     2221 cacctttcgc atgggccgcg gggaattcga gaccctgctc tcctgcgtct tcttcacggg
     2281 cctgatgctg acgatcgaat ccccccgcta cgcgcggacg gacaaggcgc tggtcacggt
     2341 cgtgtttctc ctcctcggcg gactcggcgc cttgccgctc gcgctggcca atggcggggg
     2401 cgaaagcctc gccatcctgc tgggccaggg ctccacgctg ctggtcatgg cggttctggc
     2461 cttcatctcg acgacactgg gattttattg gatggtcaaa tggcagccga acgtgacggc
     2521 gacggaggcg ggaatcgtct attgcctcga acccgtgtgg gcctccctgc tgtcgctttt
     2581 ccttccggtg atcctggccg ggctcatggg agtggcctac gcgaatgaac aaatcacgct
     2641 ggccatggtg ctcggcggcg ggcttattct ctcggccagc gtgctgctcg cgctcgtgac
     2701 tccgccgcca aaaaccgccg ctccggactt gccgaaagcc ggagcctgag cgaaggtttt
     2761 cgcaccatga aacgcactgg ctccgctcac tggcaaggca acctgaacga tggcaaaggc
     2821 accgtttcca ccccgagtgg agcgctgaag gaaacgcctt attccttcaa ggcccgcttt
     2881 gagacggaaa atggcacgaa ccccgaggaa ttgatcgccg ccgcgcatgc cggctgcttc
     2941 accatggcgc taagcctgtt tctcggcaac gcggggttta ctccggaatc gctcgatacc
     3001 gtcgccgaag tctcgctcga ccaggtcaaa agcgcctggg cagttacggc catcaagctc
     3061 acgctgaaag ccaaggtgcc gggcatcgag gaggcgaagt tccaggagct ggcggccttt
     3121 gcgaaggcga attgcccgat ctcgaagctg ctcgacgcgc cgatcacgct cgaagcctcg
     3181 ctggcagcct aacgccccag cgccagcacc ctgaagtggc ccgtctccgc gccgccggcc
     3241 cggccgaggt ggcgcgcgaa gaattcaccc tgggtgccgt caaattcgcg gcggaatccg
     3301 gcggctttcg cccaattccg gacatcgcgg aaattaacgt cggcggtaag atcctgctgg
     3361 ccaaaccgcg cgtaaatttc atcccccgaa aagcgctggt
//
LOCUS       DATDAY010000177         3111 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_84603, whole genome shotgun sequence.
ACCESSION   DATDAY010000177 DATDAY010000000
VERSION     DATDAY010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 3111)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3111)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3111
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_84603"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..715
                     /locus_tag="VIS74_03425"
     CDS             <1..715
                     /locus_tag="VIS74_03425"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY5792322.1"
                     /translation="AHFNDFEVFPIQAPTLVFVSPGQVHHWLDAHRLRGHIICFTQQF
                     FDGDLPPPSPLLKHGFWFPHETPPILPLNGESAETKTLFEEMEREFHNRSTDFESAIT
                     ALLRMLFIRAERLYRETETPLESGRSSALVRDFRLALERHFRTTQSVNDYAKLLKVSA
                     DHLGEAVHARIGRPAGDLIRQRILLEAKRLLAHTEMSVSEAAYSLNFQDPAYFSRFFR
                     RLTGQSPGEFRDSLHTQGE"
     gene            complement(780..1169)
                     /locus_tag="VIS74_03430"
     CDS             complement(780..1169)
                     /locus_tag="VIS74_03430"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HEY5792323.1"
                     /translation="MSRSIQLDTNALIALADPAGAVVQLVRERVQEGRLPAASAVAWH
                     EFVRGPLLPDELLRVERVVGARIQPVTRGTAELAARLFNATGRRAFTADCFIAASAIE
                     SGAELLTANREDFNPFVPFGLQLAAAG"
     gene            complement(1166..1450)
                     /locus_tag="VIS74_03435"
     CDS             complement(1166..1450)
                     /locus_tag="VIS74_03435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792324.1"
                     /translation="MKTTLELPDDLYQAAKTRAASQKKKVKDLISEGLRAVLTAEPEA
                     ASDQEEARRVLAALDEILRCPPSAPGRTALLQAEVRRLRSEGWNREEAGS"
     gene            complement(1478..1831)
                     /locus_tag="VIS74_03440"
     CDS             complement(1478..1831)
                     /locus_tag="VIS74_03440"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEY5792325.1"
                     /translation="MQFGIMRTGSAAHYPEHHASISGLFASCPHPSRIAEEHDSVTSL
                     MAAVEAGHGIALVAQGLDCLASSRLKIRPLQPTPPPLIVGIACRKGADSPLTRNFMAA
                     ARKASLAEALICNLP"
     gene            2092..2883
                     /locus_tag="VIS74_03445"
     CDS             2092..2883
                     /locus_tag="VIS74_03445"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY5792326.1"
                     /translation="MLLKNKNAVIYGAGGAIGGAVARAFAREGARVFLTGCLRAPVEA
                     VAKDIVSAGGSAEAAEVDALDEQAVDKHLQSVIDQAGRVDISFNAVGIPNAKILGVPL
                     TELDVEQFSLPIMAYTKSYFLTARLAARRMVANKSGVIMTVTALHSRMGIPLVGGYGP
                     AMAAKEALTRDLSAELAPQGIRVVGLRPQGMPETRTIKEAFEPRAKASGMTWEQWQEL
                     LVSRTHPRRLMTLEEMANMAVFMASDKASGMTGTTVNLTMGSLDD"
BASE COUNT          636 a         1015 c          923 g          537 t
ORIGIN      
        1 cgctcacttc aacgactttg aagtcttccc catccaggca cccacgctgg tgtttgtcag
       61 ccccggccag gttcatcatt ggctggacgc ccaccggctc cgcgggcaca tcatctgctt
      121 cacccagcaa tttttcgacg gcgacctccc cccgcccagc ccgctgctca agcacggctt
      181 ctggttcccg cacgaaaccc cgcccatcct gccgctgaat ggggaatcgg cggaaaccaa
      241 gacgcttttt gaggaaatgg agcgcgagtt ccacaaccgg agcacggact tcgagagcgc
      301 catcaccgcg ctgctccgaa tgctcttcat ccgcgcggag cgcctgtacc gcgaaaccga
      361 gaccccgctc gaatccggcc gatcctccgc gctcgtgcgg gacttccgcc tggcgctgga
      421 gcggcatttc cgcacgaccc aatccgtgaa cgattacgca aaactcttga aggtctccgc
      481 cgatcacctc ggcgaagccg tccacgcgcg catcggccgg cccgccggcg atctcatccg
      541 ccagcgcatt ctgctcgaag ccaagcggct gctcgcgcac acggaaatga gcgtgtcgga
      601 agccgcctat tcgctgaact tccaggaccc ggcctacttc agccgcttct tccgccgcct
      661 caccggccag tcgccggggg aattccggga cagcctgcac acccaaggcg aataacccgc
      721 cgctggttca tcctcgatca caccggcggg cctcaagcca gacgcattta tttcccaact
      781 cagccggctg ccgccagttg aagaccgaag ggcacgaaag ggttgaagtc ctcccgattg
      841 gccgtgagca gttccgcgcc ggattcgatg gcgctggccg cgatgaaaca atcggcagtg
      901 aaagcgcggc ggccggtggc gttaaaaaga cgggcagcca gctccgccgt gccgcgcgtg
      961 actggctgaa tcctcgcgcc cacgacccgc tcgacccgca gaagttcgtc cggaagcagc
     1021 ggcccgcgca caaattcatg ccaggccacc gcgctcgccg ccgggagccg gccttcctga
     1081 acgcgctcgc gcacgagttg aacgaccgcg ccagccggat cggcaagggc gatgagggca
     1141 ttcgtatcca gttgaatgga ccggctcacg atcccgcctc ttcacgattc cagccttcgg
     1201 accgcaaccg ccggacttcg gcttgcagca gagccgtgcg gccgggcgcg gaaggcgggc
     1261 agcgcaaaat ctcgtccaaa gccgccaaaa cccgccgggc ctcctcctgg tccgaggccg
     1321 cttccggctc cgccgtcaac accgcgcgca acccctcgga aatcaagtcc ttgactttct
     1381 tcttttggga ggcggcgcga gttttggctg cctgataaag atcgtcggga agctccaatg
     1441 tggttttcat ttccataaat atggaagatt tccactttta tggcaaatta caaataaggg
     1501 cttcagcgag gctggccttt cgggcagcgg ccataaaatt ccgggtgagc ggtgaatccg
     1561 cgcccttgcg gcaggcgatc ccgacaatga gcggcggcgg agtcggctga agcgggcgga
     1621 ttttcaatcg cgaactcgcc aggcaatcca ggccctgggc caccagcgcg atgccgtgtc
     1681 cagcctccac cgccgccatc aggctggtga cgctgtcgtg ctcttccgca atgcgggacg
     1741 ggtgcgggca cgaggcaaac aggccggaga tcgaggcgtg atgctcggga taatgcgctg
     1801 cggaacccgt gcgcatgatg ccgaactgca ccgatgtcgc agatgaaatt tgagaagcgg
     1861 ggttcgaatc cccgtgggga cgccaactta agaaggcttt tatctaaaaa acacggctga
     1921 tctctgcgcg agtaatccgc ccgagctgtt cgggtgcgcg gtgacacgtg ccggcgtggt
     1981 ggagcaaaaa aacccaaagc gaccgatgag tttggaggcg agcggcagtc taaacaacag
     2041 acgccccaaa gcgtccgccc agcactcatt caacctcaat ccaaacaacc catgctatta
     2101 aaaaacaaaa atgcggtgat ctacggagcc ggaggcgcca tcggcggcgc cgtcgcacgc
     2161 gcctttgcgc gcgagggggc cagggttttt ctcaccgggt gcctccgggc gccggtcgaa
     2221 gcggtcgcca aggacatcgt ttccgccggc ggatccgccg aggcggcgga ggtcgatgct
     2281 ctcgacgagc aggccgtgga caagcaccta cagtccgtga tcgatcaggc gggccgcgtc
     2341 gatatctcgt tcaacgcggt cggcatcccg aatgcaaaga ttctgggtgt ccctctgacc
     2401 gagctggatg tcgagcagtt ctccctgccg atcatggcct acaccaagtc atacttcctg
     2461 accgcacgtc tggcggcccg gcggatggtc gcgaataaat cgggggtgat catgaccgtc
     2521 accgcactcc actcgcggat gggcatccca ttggtgggag gctacggtcc ggcgatggcc
     2581 gccaaggagg cgctcactcg agacctatcc gccgagcttg cgcctcaggg cattcgcgtg
     2641 gtcggtctgc ggccacaggg catgccggag acacgcacga tcaaggaagc cttcgagcct
     2701 cgcgccaagg catcgggaat gacctgggaa cagtggcaag agttgctcgt aagcagaacc
     2761 cacccgcgac ggcttatgac actcgaggag atggcaaaca tggcggtctt catggcttcc
     2821 gacaaggcca gcgggatgac gggaacgacc gtcaacttga ccatggggag tctggacgac
     2881 tagcgatcgc cgcgctcgct gcgggagcca ttcatgccgc gattgcggcc tctcaacagg
     2941 ccgattagtt ttagccattc gccaagtcaa aagttaaacc tgccaggatc tcaaaattgc
     3001 ctttcgccga cacaaatcat gagcgccaaa tcgacccagc aactgccgtc gccaccaccg
     3061 ccgttgggaa ctgtgacgaa gaccgatcga aagggttttg aagagatcgt c
//
LOCUS       DATDAY010000178         6020 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_1216155, whole genome shotgun sequence.
ACCESSION   DATDAY010000178 DATDAY010000000
VERSION     DATDAY010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 6020)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6020)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6020
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1216155"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1076)
                     /locus_tag="VIS74_03450"
     CDS             complement(<1..1076)
                     /locus_tag="VIS74_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor region1.1
                     domain-containing protein"
                     /protein_id="HEY5792327.1"
                     /translation="MKKKTQPPSKSAPSKKAAPTSVKKPKASAAEKPAPPKPEPKISE
                     LAALNAAASDPQKQIQEKVRELIKLAKEQGYVTYDDLNEALPEGVNDPEAVENIINQL
                     RSVEIEIIDSSDVDRVKEVKKDGDEDEEREEKADTKLDILDDPVRMYLKQMGQVPLLT
                     REQEVEISKRIEDAELRVQDTLYRFGFTARAHLELSQKLLDLRERFDRVILDKKIDSR
                     ERYMKALPKLCQSLKKQADQVTEIYRSRIAGRGKSASEADLRTFEKVLSTLQKLYPKF
                     YFKQKIIEDFVSMSDETLRAVQNLLHENARHSPLNSAEETKAKKNIAIQLKELQSRLW
                     MTPEEFTSAHKSLRDWHKKALRAKT"
     gene            complement(1081..2868)
                     /gene="dnaG"
                     /locus_tag="VIS74_03455"
     CDS             complement(1081..2868)
                     /gene="dnaG"
                     /locus_tag="VIS74_03455"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEY5792328.1"
                     /translation="MPLISEESIRQVAEANDIVEVIGSYFPLKKAGASLRALCPFHRE
                     KSPSFHVNPARQTYHCFGCGAGGGVIKFVMEYEQLDFPAAVRNLAQRAGITLIEEISA
                     DSDRGERARLLAMHAAAADWFHQHLMRSRDAAPAREYLKGRGLTQQIAKDWKLGYAPE
                     AWDALLDFLRGQQFSAAEILCGGLTTTRENESDPAKAYDRFRARLMIPIRNDYGEVIG
                     FSGRVLDPDAKTAKYVNSPETPLFTKGRVLFGLDRTKRALIEARAAIVCEGQIDLITA
                     YESGIPNVIAPQGTAFTLQQARLLKRFVDTVILCFDSDTAGEKAVDRSLPSLFDQDLT
                     VKMILLPAGEDPDSLIRKKGADAFAKLVGEAREVFDFKLKLAEQRGEFATAQSRSQLA
                     RRFAALLGFVTDPVLRRTIVALIAARLEVPIDQLSALIPAKPALPDSEEIAPAPANEP
                     PIVLGETMATLCRLALADPEARRWLARQDPTPLRELGPDFAVLQAILDSELPAGPAFL
                     AGLPARLQNAISVLDLEKLPPNPLEMAQKYWTGMLGQHLRIRQRAAQSRLRLPNLDAT
                     QMIEIQKEILDLDQRLMDISRPISSVKPA"
     gene            complement(2960..3958)
                     /locus_tag="VIS74_03460"
     CDS             complement(2960..3958)
                     /locus_tag="VIS74_03460"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HEY5792329.1"
                     /translation="MSLTGFSSRTAAIVLFFASGRLFAQESAVDAISSKVRQLFEEKK
                     SAVVQVEAEDTRGTIRGTGFYIDAMGTLFTLAAMADGTENVSVIAGGKKIPAKVLACD
                     TRSGIALLQTAGCSAFIVPGDSSQLAIASPVVMIGYPLDLDLSPGFGLISGFDRKFAD
                     VYFSTTHLRVNVPVMRGQGGSPILNMDGQVVGIVISSVDNGATCYVLPINAAEKILSD
                     YARFGEARHGWVGVKVDPAGEPVQGSSAVIGELDPETPASKSGLKKGDVLLRVGDIEI
                     HKPEDVLDASFFLTAGDTTEIQVLRDGKPVTLGVETVLHPWASKPALQAGPSLMGN"
     gene            complement(3939..4373)
                     /locus_tag="VIS74_03465"
     CDS             complement(3939..4373)
                     /locus_tag="VIS74_03465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792330.1"
                     /translation="MSDETQIQKLLRLKRYELPPEGYYTDFLREFQRRQRAEVLKSSV
                     WEILWDRLGHVLPDFRVPTYAYAAIGLMAVAASSFILTTGEVSPPLAVRSADPGVTFD
                     LNSKAAELQQPVEIPATRVVGTLPPHYVLQPRPAVKDEPYRF"
     gene            complement(4381..4965)
                     /locus_tag="VIS74_03470"
     CDS             complement(4381..4965)
                     /locus_tag="VIS74_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY5792331.1"
                     /translation="MESPETPDDVLISRTREGDPSAFDELVIRYTPRIYGLVYNMTSN
                     HEDTNDLLQEVFAKAYRSIHGFKGNSSFSTWLHTIAVNRTINFVKKRGRRQQMSLDDV
                     DANIENDPDFIEATASSDPVRETNLNELQKRLNEAMQKLSPNHRAVVTMFDIQGMPHA
                     EIAKVLGTSEGTVRSRLFYAHRELQNYLEEFRRS"
     gene            5071..5886
                     /locus_tag="VIS74_03475"
     CDS             5071..5886
                     /locus_tag="VIS74_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006983369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY5792332.1"
                     /translation="MTTCLAPLPLWPESAPGALGTDEKDIPTLAPYLPEPEIATGAAM
                     VVFPGGGYGMLADHEGEGYAKWLARHGIAALVVKYRLGSHGYRHPVMLNDAARAVRLA
                     RALAGEWDINPGRIGVIGSSAGGHLVSTLATKFDLGNPASPDPVERESARPDLAVLCY
                     PVITMGVHTHHGSKTHLLGAAPSDESVALLSSERQVTPGTPPCFVWHTWEDGAVVVEN
                     SLGFAAALREKGVRFELHVYERGGHGLGLGSPENPHRWTADCLAWLRERGFAR"
BASE COUNT         1067 a         1765 c         1931 g         1257 t
ORIGIN      
        1 gtcttggccc ggagcgcctt cttgtgccag tcgcgcaggc tcttgtgggc cgaggtgaat
       61 tcctccggcg tcatccagag gcggctctgc agctccttga gctgaatggc gatattcttc
      121 tttgccttgg tctcctccgc cgagttcaag ggggagtgcc gggcgttttc gtgcaggaga
      181 ttctgcacgg cgcgcagcgt ttcatcggac atggacacaa aatcctcgat gatcttctgc
      241 ttgaaataga atttcggata cagcttctgg agcgtcgaaa gcaccttctc gaaggtccgg
      301 agatcggcct cgctcgcgga tttgccgcgg ccggcgatgc gggagcggta gatttccgtc
      361 acctgatcgg cctgcttctt gaggctctgg cagagcttcg gcagcgcctt catgtaacgc
      421 tcgcggctgt cgattttttt gtcgaggatc acgcggtcga agcgctcgcg gaggtccagg
      481 agtttctggg agagctcgag gtgggcgcgg gcggtgaagc caaaccggta aagcgtgtcc
      541 tgcactcgca gctcggcgtc ctcgatccgt ttggaaattt ccacttcctg ctcgcgggtg
      601 agcagcggca cctggcccat ctgcttgaga tacatccgga ccggatcatc caaaatgtcg
      661 agcttggtat cggctttctc ctcgcgttcc tcatcttcgt cgccgtcctt tttgacctcc
      721 ttgacgcggt ccacgtcgga ggaatcaatg atttcgattt cgaccgagcg gagctggttg
      781 atgatgttct cgacggcttc cggatcgttc acgccttccg gaagcgcttc gttgagatcg
      841 tcataagtga cgtaaccctg ttccttggcg agcttgatga gttcgcggac tttttcctga
      901 atttgcttct gcgggtcgga ggcggcggca ttgagcgcgg cgagctcgga aattttgggt
      961 tccggcttgg gcggggccgg cttttcggcg gccgacgcct tgggtttttt tacggaagtg
     1021 ggggctgcct ttttggaggg agcagacttg gatggtggct gagtcttctt tttcaatggg
     1081 ttaggcaggt ttgaccgagg aaatgggccg cgaaatatcc attagcctct gatcaaggtc
     1141 aagtatttct ttctggattt cgatcatctg ggttgcgtcg agattgggaa gtcgcaaccg
     1201 gctttgggcg gcccgttggc gtattcgcaa atgttgtcct agcatacccg tccaatattt
     1261 ttgagccatt tcaagtgggt ttggcggcaa tttttccaga tcgaggacgg aaatcgcgtt
     1321 ttgcagacgg gcgggcaggc cggcgaggaa ggcgggaccc gccggaagct cgctgtccag
     1381 gattgcctgg agcacggcga agtccggtcc gagttcccgc aagggggttg gatcctggcg
     1441 ggcgagccag cgccgggctt ccgggtcggc cagggcgagg cggcagagcg tggccatggt
     1501 ttcgcccaac acgatcggcg gctcgttcgc gggcgcgggc gctatttcct ccgaatcggg
     1561 cagggccggt ttggcgggga tcagggcgct gagctggtcg atgggtacct ccagccgcgc
     1621 ggcgatcagg gcgacgatcg tccgccgcag cacgggatcg gtgacgaatc cgagcagcgc
     1681 ggcgaaccgc cgggcgagct ggctccggct ctgggcggtg gcgaactccc cgcgctgctc
     1741 ggcgagtttc agtttgaaat caaagacctc gcgggcttcc ccgaccagtt tcgcaaaggc
     1801 gtccgcgcct ttttttcgaa tgagggaatc cggatcctcc ccggcgggca gcaaaatcat
     1861 tttcaccgtc agatcctggt cgaacagcga cggcagcgag cgatccaccg ctttctctcc
     1921 cgcggtgtcc gagtcgaagc acaggatgac cgtatccacg aaccgtttca acagccgcgc
     1981 ctgctggagc gtgaaggccg tgccctgggg ggcgatgacg ttcgggatgc cggattcata
     2041 ggcggtaatg agatcgatct gcccctcgca gacgatcgcc gcgcgcgcct cgatcagcgc
     2101 gcgcttggtc cggtcgaggc cgaagaggac gcggcccttg gtgaagagcg gcgtctccgg
     2161 ggaattgacg tatttggcgg ttttcgcatc cggatccagc acccggccgc tgaatccgat
     2221 gacctcgccg tagtcgttgc ggatcggaat catgagccgc gcgcggaagc ggtcataggc
     2281 tttcgcgggg tcggattcgt tttcgcgggt ggtggtgagg ccgccgcaca ggatctcggc
     2341 ggcgctgaat tgctggccgc gcagaaaatc cagcagcgcg tcccacgcct ccggcgcgta
     2401 gcccaatttc caatccttcg cgatctgctg ggtgagcccg cggcccttga ggtattcccg
     2461 cgcgggcgcg gcgtccctgg agcgcatcag gtgttgatga aaccagtccg ccgccgccgc
     2521 gtgcatcgcc agcaggcggg cccgctcgcc ccggtccgag tccgcggaaa tttcctcgat
     2581 cagcgtaatg ccggcccgct gggcgaggtt ccgcacggcg gcgggaaaat ccagctgctc
     2641 gtattccatc acaaacttga tcacgccgcc gcccgcgccg cagccgaagc agtgataggt
     2701 ttgccgcgcc ggattgacgt gaaaggaggg gcttttttcg cgatggaacg ggcaaagcgc
     2761 ccggaggctg gcgccggctt tcttcagcgg aaaataggag ccgatgacct cgacgatgtc
     2821 attcgcttcg gcgacctggc ggatgctttc ttcggaaatg agcggcacgg aactaagcag
     2881 gattgataag attggcggga tgaacaggct ttttccttaa aatcttcatc ccgttaatcc
     2941 ggcaaatccg gaaatcccgt tagtttccca tcaatgacgg gccggcctgc aaggcgggct
     3001 tgctggccca gggatgcagc acggtctcca cccccagcgt gacgggcttg ccgtcgcgga
     3061 gcacctgaat ttccgtcgtg tcgccggcgg tcaggaaaaa ggacgcatcg agcacgtcct
     3121 cgggtttgtg gatttcgata tcgccgacgc gcagcaggac atcgcccttt ttcaatccgg
     3181 atttggaggc gggcgtctcc ggatcgagct ccccgatcac ggcgctggaa ccctgaaccg
     3241 gctcgccggc gggatccacc ttgacgccca cccagccgtg gcgggcttcc ccgaagcggg
     3301 cgtagtcgga aaggattttc tccgcggcat taatgggcag cacgtaacag gtggctccat
     3361 tatccacgct gctgatgacg atgccgacca cctggccgtc catgtttaga atgggagagc
     3421 ccccctggcc tcgcatcacc ggcacgttca cccgcaggtg ggtggtcgaa aaatacacat
     3481 cggcgaattt gcggtcgaag ccggaaatga ggccgaagcc gggcgagagg tcgagatcga
     3541 gcggatagcc gatcatgacc accggggagg cgattgccag ctggctggaa tctcccggca
     3601 caatgaacgc cgagcagccc gcggtctgca gcagggcgat gccgctgcgc gtgtcgcatg
     3661 cgagcacctt ggcgggaatc tttttgccgc cggcaatgac cgagacgttt tcggtgccgt
     3721 ccgccatggc cgccagcgtg aacagcgtgc ccatggcatc gatgtaaaag ccggttcccc
     3781 tgatggtgcc gcgagtgtcc tccgcctcga cctggacaac cgcggatttt ttttcctcaa
     3841 agagttgccg gaccttgctg gaaatcgcat ccactgcgct ttcctgggcg aagagccgcc
     3901 ccgaagcgaa aaaaagaacg atggcggcgg tgcgggaact aaaaccggta aggctcatct
     3961 ttcaccgcgg gccggggttg gaggacgtaa tgcggcggca gggtgcccac cacgcgggtg
     4021 gcgggaattt ccaccggctg ctgaagctcg gcagccttgg aattaagatc aaaggtgact
     4081 cccgggtccg cggagcggac ggcgagcggc gggctgacct cgcccgtggt gagaatgaag
     4141 gacgaagcgg ccaccgccat gagcccgatg gcggcatacg cgtaagtggg cacccggaag
     4201 tccggcagca catgccccag gcggtcccag agaatttccc acaccgaact cttcagaacc
     4261 tcggcccgct ggcggcgctg gaattcccgc aggaagtccg tgtaatagcc ctcgggaggc
     4321 agctcatagc gtttcagccg cagcaatttt tggatctggg tttcgtcact catggtgggc
     4381 ttagcttcgt ctaaactctt caagataatt ttgcaactcg cgatgcgcgt aaaaaaggcg
     4441 ggagcgaacg gttccttcgg aggtgcccag caccttcgcg atctccgcat gcggcatgcc
     4501 ttgaatatca aacatcgtca ccactgctct gtgattagga gacagtttct gcatggcctc
     4561 gttcaatctt ttttgaagtt cgttgaggtt ggtctcgcgc acgggatcgc tggaagccgt
     4621 ggcttcgatg aaatcgggat cgttctcgat attcgcatcc acgtcgtcca ggctcatctg
     4681 ctggcggcgg ccccgctttt tgacaaaatt gatcgtccgg ttcaccgcga tcgtgtgcag
     4741 ccaggtggag aagctggaat ttcccttgaa gccgtgaatg gagcgatacg ccttcgcgaa
     4801 aacctcctgc aataaatcgt tcgtgtcctc gtgattcgag gtcatgttgt aaaccaggcc
     4861 gtaaatccgc ggagtatagc ggatcaccag ctcgtcgaac gccgacggat ctccctcgcg
     4921 cgtgcgggag atcaggacat catcgggcgt ctcgggcgat tccattgccc gaaattgctt
     4981 aacacagctt ttttccccag cggtagaaaa aactgttcgt tttggaaaag gcgtgatttg
     5041 gcgcaagggt ctggcgtatt ccccatttgc atgacgacct gccttgctcc gctgcccctc
     5101 tggcccgaat ccgctcccgg cgctttgggg actgacgaaa aagacatccc cacgctggcc
     5161 ccctatctgc ccgaaccgga aatagccacc ggcgcggcga tggtggtttt tcccggcggc
     5221 ggatatggca tgctggccga tcacgaaggc gaaggttacg ccaaatggct ggcgcggcac
     5281 ggcattgccg ccctggtggt caaataccgc ctcggcagcc acggctatcg tcacccggtg
     5341 atgttaaatg acgccgcgcg ggccgtgcgc ctcgcgcgcg ccctggccgg ggaatgggat
     5401 atcaaccccg ggcggatcgg cgtcatcggc tccagcgcgg gcggccatct ggtttccacg
     5461 ctggcgacga aatttgattt gggcaatccc gcctcgcccg atcccgtcga gcgggaaagc
     5521 gcccggccgg atctggcggt gttgtgttat ccggtcatca ccatgggagt gcatacccat
     5581 catggatcga agacccattt gctcggcgcc gccccctcgg acgaatcggt ggcgctgctc
     5641 tccagcgaac ggcaggttac tcccggcacg ccgccgtgtt ttgtctggca tacgtgggag
     5701 gacggcgccg tggtcgtgga aaactccctg gggtttgcgg ccgcgctgcg ggaaaagggc
     5761 gtgcgtttcg agctgcacgt ttacgagcgc ggcgggcatg gcctcggcct cggttcgccg
     5821 gaaaatccgc atcgttggac ggcggattgc ctcgcctggc tgcgcgaacg cgggttcgcg
     5881 cgttgagtcc tgtagccggg atcggtgatc ccggccgatt gatttccgga tctgaacctg
     5941 ccgggaacgc cgatcccggc tacagcgaaa attccttacg ccagtcccgg cagcgtttcc
     6001 gtgagcagcc ggaccaaatt
//
LOCUS       DATDAY010000179         5095 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_2311819, whole genome shotgun sequence.
ACCESSION   DATDAY010000179 DATDAY010000000
VERSION     DATDAY010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 5095)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5095)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5095
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2311819"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..392)
                     /locus_tag="VIS74_03480"
     CDS             complement(<1..392)
                     /locus_tag="VIS74_03480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792333.1"
                     /translation="MHAHEIFQQLSAEGSAALFEHLYTADRPAYRAAAQILAARRKLR
                     PIFVERKPRPERDQWLREALSRRPNEDAALEVLQAWLLGAHRDLICTFLDDMGVGHDG
                     KGLIEELPDEPPEAKLAGGVDRLLAGFDR"
     gene            549..1475
                     /locus_tag="VIS74_03485"
     CDS             549..1475
                     /locus_tag="VIS74_03485"
                     /inference="COORDINATES: protein motif:HMM:NF017022.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChuX/HutX family heme-like substrate-binding
                     protein"
                     /protein_id="HEY5792334.1"
                     /translation="MIPRHRLRAFRSGPRACPSPIARHAAEENLSHTAAGETRVALGR
                     DWLPMLADLREVGPVLSITRNSHAILGREGPLPELERDREGASAGDAGGEFDFEFDHW
                     GRARAVHAKTAHGHLYSVEWLDAHGAVHHKTCLTSAADPRQFAGWVACHQHSPERPAP
                     PNPSARRGETSAEPGRAIHRGCVDYLLREMISLAQPVRVIVGNEASVQGHVFTPTLLR
                     HSDEWCFVSGGQTGLHLRTRNLAEVLLLETATSAGTFAVLKLYEPEGRLAAVIAPPLD
                     ADFSQWNAFIDGRIVFQPPTSSRQSPNHPFHT"
     gene            1472..1930
                     /locus_tag="VIS74_03490"
     CDS             1472..1930
                     /locus_tag="VIS74_03490"
                     /inference="COORDINATES: protein motif:HMM:NF012481.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HEY5792335.1"
                     /translation="MSEPITILYATMTGNSRDCAENTVKRLKQEGHTATAIDLAHYKV
                     GDISNEKVVLLTISTWGEGEAPDDGVNFLEHVKSLSAGSLPGLEFSVLALGDTSYDNF
                     CQCGKDFDTALEALGAKRLAPRMDCDVDFQENADAWLESVVAALAGKVAA"
     gene            1958..2470
                     /locus_tag="VIS74_03495"
     CDS             1958..2470
                     /locus_tag="VIS74_03495"
                     /inference="COORDINATES: protein motif:HMM:NF012436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HEY5792336.1"
                     /translation="MITSQPVIQLLNEQIRNEFQSHQIYLALSAWFETTPYKGFAAKY
                     RQQALEEHGHALKIYNFLADRDGRIRILALEEPRLKIQSVLDAAKSALEQEQAVSAQI
                     RNIYKVAQTEEDFETLSFLKWFLDEQVEEERTAKDLVEYVQAAGASTTALLELDQHAA
                     TEAAKPSPAV"
     gene            complement(2519..3679)
                     /gene="tcmP"
                     /locus_tag="VIS74_03500"
     CDS             complement(2519..3679)
                     /gene="tcmP"
                     /locus_tag="VIS74_03500"
                     /inference="COORDINATES: protein motif:HMM:TIGR04474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="three-Cys-motif partner protein TcmP"
                     /protein_id="HEY5792337.1"
                     /translation="MSPQDDELYFRREQSQIKHSILDKYLERFARIVGKRWDGILYVD
                     GFSGPWNINGDDFRDSSFDIALRQLRSARDTVRKTFQKDLKIKCIFLEKDAAAFSQLQ
                     NYAKQQTDVEVIPLNRDFEQAVPELERMIRKESQGFFPFIFIDPTGWAGFSMKLIAPL
                     IQIRPCEVIINFMTSFIQRFINDERAGIEASFKKLFGDDFYKQEIDGREGLDREDAIV
                     FTYAKRIAEVGGFDHVPITVVLHPTKDKSHFHLVYAMRNIAGLKVFKEAEQHALKLSE
                     QVRADAKRRAREASSGQSELFGGREIPETIYASELLEHYESLARSSVHELIRDKKEVS
                     YNNVYAIGMQFPMVQEKSLRSWITEIADVINLSPMKKVPKLNSEHRIRFRQI"
     gene            complement(3685..4473)
                     /locus_tag="VIS74_03505"
     CDS             complement(3685..4473)
                     /locus_tag="VIS74_03505"
                     /inference="COORDINATES: protein motif:HMM:NF019146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage Gp37/Gp68 family protein"
                     /protein_id="HEY5792338.1"
                     /translation="MSASSKIEWTDATWNPVRGCSKVSPGCKHCYAETFAERFRGVKG
                     HPFEFGFDLRLVPEKLGDPIRWSTPRRIFVNSMSDLFHEDVSDDYIEKICRVMLAANW
                     HTYQILTKRADRMSELLRTKLRKAANTSHIWWGVSVENRKQGLPRIELLRKARPHIAF
                     LSVEPLLEDLERFDLTNIHWVIVGGESGHGARLLDEKWVRSIRKQCQTAQIPFFFKQW
                     GGVRKAETGRILDGQTYDEFPRIVSNPVASAMNRIRLLEELVTA"
     gene            complement(4470..4973)
                     /gene="lexA"
                     /locus_tag="VIS74_03510"
     CDS             complement(4470..4973)
                     /gene="lexA"
                     /locus_tag="VIS74_03510"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: protein motif:HMM:TIGR00498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HEY5792339.1"
                     /translation="MQLDTSSKAAADHIAALKRKGYLADDSEGGRGLRLTEKAIGIPV
                     LGDIPAGSPVDVEERVEFSLPINTRPFGVSDRNSAFFLRVRGDSMIGRHIFDGDLVLV
                     EKSNRFKNRDVVAALIDNESTLKTFIRDAGQTWLKSENPKYPNLIPALDLQVQGVARS
                     VIRLFPS"
BASE COUNT         1135 a         1542 c         1392 g         1026 t
ORIGIN      
        1 cggtcaaatc cggccagcag ccggtcaacc cccccggcca atttcgcctc gggcggttcg
       61 tcgggcagtt cttcgatgag gcccttgcca tcgtgcccga cccccatatc gtccaaaaac
      121 gtgcagatca aatcgcggtg cgcgcccagg agccaggcct gcaggacctc gagggcggcg
      181 tcttcattgg gccggcggct gagggcctcg cgcagccatt gatcccgctc gggccggggt
      241 ttgcgttcga caaaaatggg ccgcagcttg cggcgggcgg cgagaatctg cgccgcggcg
      301 cggtaggccg gccggtcggc ggtgtaaagg tgttcaaata gggccgccga tccctcggcg
      361 gaaagttgct ggaaaatttc gtgcgcgtgc atgcggcgga gtttgggagc ggcgtttaat
      421 ccatcacgtg cccgccgggg gttcaatcca aaaccgcggc ccggcccgat tcctccgggc
      481 gcaaatccgc gtcaattttt cgcaatcccc catcagcgtc ccggatcaaa tcctgctacc
      541 ccggcgggat gatcccacgc caccgcctcc gcgccttccg ctccggcccc cgcgcctgcc
      601 cgagtccgat cgcgcggcac gcggcggagg aaaacctttc ccataccgcc gccggcgaaa
      661 cccgcgtggc gctgggccgc gactggctcc cgatgctggc ggacctccgg gaagtcgggc
      721 cggtgctctc cattacccgg aattcgcatg ccatcctcgg ccgggaaggt ccgctgccgg
      781 aactcgaacg cgaccgggaa ggcgcgagcg ccggcgacgc gggcggggaa tttgacttcg
      841 agttcgatca ctggggccgc gcccgggcgg ttcacgctaa aaccgcccat ggccaccttt
      901 actccgtgga atggctcgac gcgcatggcg ccgtccatca caaaacctgc ctgacttccg
      961 ccgccgatcc ccgccagttc gccggctggg tggcctgcca ccagcattcg ccggaacggc
     1021 cggccccgcc caatcccagc gcccgccgcg gcgaaaccag cgcggaaccc ggccgggcga
     1081 ttcaccgcgg ctgcgtggat taccttctcc gcgagatgat ttccctcgcg cagcccgtgc
     1141 gggtcatcgt cggaaacgag gcctcggtcc agggccacgt ttttacaccc acgctgctcc
     1201 ggcactcgga cgaatggtgt ttcgtgagcg gcggccaaac cggcctccac ctgcgcacga
     1261 gaaatctggc ggaggtgctg ctcctcgaaa ccgccacctc cgcgggaacc tttgccgtat
     1321 tgaaacttta cgaaccggaa gggcggcttg ccgccgtcat cgccccgccg ctggatgcgg
     1381 atttttccca gtggaatgcc ttcattgatg gccgtatcgt tttccagcca ccgacttcct
     1441 cccgccaatc acccaaccat ccattccata catgagcgaa cccatcacca tcctctacgc
     1501 gaccatgacc ggcaattccc gggattgcgc cgaaaacacc gtcaaacgcc tgaagcagga
     1561 aggccatacc gccaccgcca tcgatctggc ccattacaag gtcggcgaca tcagcaacga
     1621 aaaagttgtc cttctaacca tcagcacctg gggcgaaggc gaagcgccgg atgatggcgt
     1681 gaacttcctc gaacacgtga aatccctgtc cgccggttcg ctgcccggcc tggaattttc
     1741 cgtgctcgcg ctcggcgaca cgagctacga caacttctgc caatgcggca aggacttcga
     1801 caccgccctg gaagccctcg gcgccaagcg cctggccccg cgcatggatt gcgacgtgga
     1861 tttccaggaa aacgccgacg cgtggctgga atccgtggtc gccgccctcg ccggaaaagt
     1921 cgccgcctga gattcaccct tcaccccccc aaaccccatg atcaccagcc aacccgtcat
     1981 ccaattgctc aacgagcaaa tccgcaacga attccaatcc caccagattt acctcgccct
     2041 ttcggcctgg tttgagacga cgccctacaa gggattcgcc gccaaatacc ggcagcaggc
     2101 gctggaggaa cacggccacg ccctgaaaat ttacaacttc ctggcggacc gggatggcag
     2161 gatccggatt ctcgcgctgg aggagccccg gttgaaaatc caatccgtcc tcgatgccgc
     2221 caaaagcgcg ctggaacagg agcaggccgt gagcgcgcag attcggaaca tctacaaggt
     2281 cgcccagacc gaagaggatt tcgaaacgct ctccttcctc aaatggttcc tcgacgagca
     2341 ggtggaggaa gaaagaaccg ccaaggatct cgtggaatac gtccaggcgg cgggcgcttc
     2401 caccaccgcc ctgctggagc tcgaccagca cgccgccacc gaagcggcca agccctcgcc
     2461 cgccgtttag cgtcgcttaa cggaatttcg tgacctgcta acaacagaac aggcaaattc
     2521 aaatttgcct gaatcgaata cggtgctctg agttcaactt cggaaccttt ttcatcggac
     2581 tcaggtttat gacatccgcg atctcagtga tccacgagcg aaggcttttt tcctgaacca
     2641 ttgggaactg catgccaatc gcataaacgt tgttgtagga aacctctttc ttgtctcgaa
     2701 tgagttcatg gacggatgac cttgcaaggg actcgtaatg ttcgaggagt tcgctcgcat
     2761 agatcgtttc cggaatctcc cttccgccaa aaagctctga ttgcccactg ctcgcctcgc
     2821 gagcacgccg tttggcgtcc gcccggactt gctccgaaag cttgagagca tgctgctcag
     2881 cctctttgaa gactttgagt cccgcgatgt tacgcatggc atataccaga tggaaatggc
     2941 ttttgtcctt cgtgggatga agaaccaccg taatgggcac gtgatcaaaa cctccgactt
     3001 cggcgatccg tttggcgtag gtaaaaacaa tagcatcctc acggtcgagg ccctcgcgtc
     3061 cgtcgatttc ctgtttatag aaatcgtcgc caaacaattt tttgaagctg gcctcgatac
     3121 ctgcacgttc atcattgatg aatcgctgga taaaactggt catgaaattg atgatcactt
     3181 cacaggggcg aatttgaatg agcggagcga tcagcttcat ggaaaaacca gcccaaccgg
     3241 tcggatcgat aaaaatgaag ggaaaaaaac cctgggattc tttgcggatc atacgctcca
     3301 gttcgggcac agcctgctca aagtcccggt tgagcggaat gacctcaaca tccgtttgtt
     3361 gcttggcgta gttctgaagc tgggagaaag cagcggcatc tttttcgaga aagatgcatt
     3421 tgatcttcaa atccttctgg aaagttttcc gcacggtatc ccgagcgcta cggagttgcc
     3481 gaagcgcgat gtcgaaagaa gagtcgcgaa aatcgtcgcc attgatattc caaggccccg
     3541 aaaaaccatc aacatagaga atgccatccc acctcttgcc gacgattcgg gcgaatcgtt
     3601 cgagatattt gtcgaggatc gaatgtttga tctgcgactg ctcgcggcgg aaatataatt
     3661 cgtcgtcctg gggagacatc ggaatcaagc ggtgactaat tcttcaagaa ggcggatgcg
     3721 attcatcgcg ctggcaaccg gattggacac gatgcgcgga aattcatcgt aagtttgacc
     3781 atccaaaata cggcctgttt ccgccttgcg aacaccgccc cattgtttga aaaagaacgg
     3841 aatttgggcg gtctggcatt gtttgcgaat ggaacggacc catttttcat ccaacaggcg
     3901 agcgccatga ccgctctccc cgcctacgat cacccaatga atgttggtta gatcgaatcg
     3961 ttccaaatcc tccagcaacg gttccacaga aagaaaagcg atgtgaggac gggccttgcg
     4021 gagcagttcg atgcgtggca acccctgctt gcggttttcg acgctgaccc cccaccagat
     4081 atgcgaggta ttggcggctt tgcgcagctt ggttcgaaga agctcgctca tcctatcggc
     4141 cctttttgtg aggatttgat acgtgtgcca attagctgcc agcatcaccc ggcaaatctt
     4201 ctcaatgtag tcgtcggata catcctcatg aaagaggtcg ctcattgaat tgacaaaaat
     4261 ccgacgcggc gtgctccagc gaatgggatc tcccagcttt tccggaacca gacgcagatc
     4321 gaatccgaac tcaaagggat gtcccttcac cccgcgaaaa cgttccgcaa aagtttccgc
     4381 ataacagtgc ttgcaaccgg ggctgacctt cgagcatccc cgaacgggat tccaagtcgc
     4441 atcggtccac tcgattttgg aagaagcgct catgatggaa aaagtcggat gacgctgcgg
     4501 gcaacgccct gaacttggag gtccaaagcc ggaatcaggt tcggatattt cggattttcg
     4561 cttttcagcc aggtttggcc agcatcacga ataaatgttt tgagtgtgga ttcgttgtcg
     4621 atcaaagccg ccaccacatc ccgattctta aaacggttgg atttttcgac cagcacgaga
     4681 tcgccatcaa agatatgacg cccaatcatg gagtcgccac ggactcgaag gaaaaacgca
     4741 ctgtttcggt cggaaacgcc aaaaggacgc gtgttgatag ggagggagaa ttcaaccctc
     4801 tcctctacat cgactggact gccggccgga atgtctccca aaaccggaat gccaatggct
     4861 ttttcggtca gacgaagccc cctgccgcct tccgaatcat cggccagata gccttttcgc
     4921 ttaagagcgg cgatgtgatc agcagccgct ttggaactag tgtctaattg caaaagacag
     4981 atagattaag taaagagaag ggatgagtcg ccgagcgccc gaagtgtcgt gttccgagga
     5041 agatcgcaaa gagcttgaag ggatgagtcg aagcgggagg gaggaagcgc ggatg
//
LOCUS       DATDAY010000180         6530 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_1575042, whole genome shotgun sequence.
ACCESSION   DATDAY010000180 DATDAY010000000
VERSION     DATDAY010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 6530)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6530)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6530
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1575042"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..215)
                     /locus_tag="VIS74_03515"
     CDS             complement(<1..215)
                     /locus_tag="VIS74_03515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792340.1"
                     /translation="MSDAGILPDLQACVLCEDVRAEISGQQTLIGVIGAIPAPILPIG
                     FMKLCLWTRWCGGTGSFTQRSLILSAD"
     gene            complement(212..1054)
                     /locus_tag="VIS74_03520"
     CDS             complement(212..1054)
                     /locus_tag="VIS74_03520"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: protein motif:HMM:NF014703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEY5792341.1"
                     /translation="MEWWQSLILGLVEGLTEYLPVSSTGHLLLTQRLLGISASDAADA
                     YAICIQVGAILAVLGLYYKRVLQMARGALGQDPAGWKLGWNILLAFLPAAVIGFLLEK
                     LIKKYLFGGDTWGLWPVVAAWFVGGVAILAVAWMRRSSNGPAKPGLALEQLTIPMAVA
                     IGLGQCLAMWPGVSRSLVTIVGGVLAGLSLPAAVEFSFLLGVLTLSAATAYDFLKHGH
                     DMLAQFGTGTLVTGLAAAWISAVLAVKWMVGYLNRHGMGLFGYYRIGIAAVVAILLLG
                     GFLK"
     gene            complement(1093..1431)
                     /locus_tag="VIS74_03525"
     CDS             complement(1093..1431)
                     /locus_tag="VIS74_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019517557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HEY5792342.1"
                     /translation="MKKIEAIIKPFKMEDVKEALAEIGIEGMTVSEVKGFGRQKGHTE
                     IYRGSEYTVDFLPKVKFEIVVADGLVEKTVAAISTAAKTGKIGDGKVFILPIEHAVRI
                     RTEETGENAI"
     gene            1530..2087
                     /locus_tag="VIS74_03530"
     CDS             1530..2087
                     /locus_tag="VIS74_03530"
                     /inference="COORDINATES: protein motif:HMM:NF021363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2062 domain-containing protein"
                     /protein_id="HEY5792343.1"
                     /translation="MRPIEWRRTRVGRYLRHLPRPKHLRGSWLHRKLGDSLLARDLWH
                     PDPSRVAAGFSLGAFFAVMPIPFQSIPAALLAFLTRSNIAAALCAVWITNPFTAPFFI
                     YAQYRIGAWILRDPNPIRFQFQQAIDWNQFFSEAPKAFLAGSVAVGLISAAVVYPIVL
                     VAWKGLALWVRSAHRKHPSAGTGDD"
     gene            2144..2764
                     /locus_tag="VIS74_03535"
     CDS             2144..2764
                     /locus_tag="VIS74_03535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792344.1"
                     /translation="MKFLLLPLVLAACLASAHAERLIPSSVDPASAAAREKAFIPWST
                     PEESSAFWKKLSPNHVPVYYERKDGPLSRDIYIPNPGIGYWVLGGLTEKQLFKIHREK
                     LEIDDTLISASAYPDERGNRLYWALWAPRERAYLLTDPMKKLGIGQARIEFSWADRFL
                     LWAAEMKPFSGFITAASLALNGLLFLLVGAIVLGGKASRINSSSPG"
     gene            2842..4455
                     /locus_tag="VIS74_03540"
     CDS             2842..4455
                     /locus_tag="VIS74_03540"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEY5792345.1"
                     /translation="MIRRLLPILLLLAGNCAAPAAASKPLGEAAAGLAAQLPKGGIVT
                     GESAGGPAAYALAGNVGVAGVPPERILFEIGSISKVFTGLLLAQAVVEGRVKLDAPIR
                     DLLEPKQTFADARVGAITLRQLAAHTSGLPRLPEELDQGANPGDPYAHYDRARLEAGL
                     ARLQLAGDAPFPRSYSNYGAGLLGDLLSRLYGKPWETLVREKIAGPLGLPDTVVRLDA
                     DQQRRFAPPFSGNSPAHPWNLASLEGAGALRSTAADLMAFGAALLHPGQTPLREALEL
                     LLAPQSADGAIGLGISLSRLFGERGYEHSGGTGGFASGLYVLPESGSVKVILINNAVL
                     SDGAVLAATHSSAPPRESARAFAADELAEFTGVYPLDSMSRFTLLLRGGQLWVRLSGQ
                     PFFPLHPHATPDRFFLKVVPAEIQFERKEGAIQSLTLFQNGRETQAAKSPEPAPVIDF
                     RSDRELASLAGAYAVNPEMNFTVKVRHSTLFVKLNQQPALPVFETSKDWFEYDVVSAA
                     LEFERDKENRVVALWLFQNGAAFRAAKKD"
     gene            complement(4610..5293)
                     /locus_tag="VIS74_03545"
     CDS             complement(4610..5293)
                     /locus_tag="VIS74_03545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792346.1"
                     /translation="MENAEQADDTLPSQGKVFRAILKVAWLAVLLGLGVEAILLAAKV
                     SLGIDTAAGRFAADVVGKITWSVLICIGLAVGIIVTRTAALPMLLFGLISAPIATILA
                     RVTHKSILEAMNLAAPGTLAGTLAVSAIRAVEYGLLGLALCLLVRKSVQSPWAYAGTG
                     FGLGLIFGLMVQGTYVWLTKAPLSPPETMARLVNEIIFPIGCSLVLYVSVAIGKSKAD
                     SLEIGSHSG"
     gene            5475..5547
                     /locus_tag="VIS74_03550"
     tRNA            5475..5547
                     /locus_tag="VIS74_03550"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5508..5510,aa:Ile,seq:cat)
     gene            5592..>6530
                     /locus_tag="VIS74_03555"
     CDS             5592..>6530
                     /locus_tag="VIS74_03555"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019924.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HEY5792347.1"
                     /translation="MAASEMAPFARTGALADGMAALPSALRQAGHDVRVVLPYYRTIR
                     DGRDFSVRPTKIRFAVPVGEARYDCEIYEARAADGVQVFFIGRDEFFDRSGLYGSSGR
                     DYQDNAARFIFFNKCVIELAKRLTPVPDILHAHDWQAALLPLLAKSQSLPWPVVLSVH
                     DPAYQGNFWSYDFGLTNLPPDYFSARGLEFYGSMNFLKGGILFADRVVLPSELYVSEA
                     QTREFGCGLDAVFRENAGKLAGIPDGVDVTQWDPASGRHLAAPYSAAGLGGKRACRDQ
                     LLAQFSLQANPKGPVFAMVSRLVQEKGLDILLPALDR"
BASE COUNT         1176 a         2074 c         2043 g         1237 t
ORIGIN      
        1 tcgtccgcgc tcaggatgag cgagcgctgc gtaaagctgc cggtgccgcc gcaccagcgc
       61 gtccacaggc agagcttcat gaagccaatg ggcagtatcg gcgcgggaat ggcgccgatg
      121 acgccgatca gggtctgctg cccgctgatt tccgcgcgca catcctcgca cagcacgcag
      181 gcctgcaggt cggggaggat gccggcatcg ctcatttgag aaatccgccc agcaggagaa
      241 tggccacgac ggccgcgatg ccgatgcggt aatagccgaa aagccccatg ccgtggcggt
      301 tgaggtatcc caccatccat ttcaccgcca ggaccgcgga aatccacgcc gcggccaggc
      361 cggtcaccag cgtgccggtg ccgaactgcg cgagcatgtc gtggccgtgc ttgagaaaat
      421 catacgccgt cgcggcggac agggtgagca cgcccagcag gaagctgaac tccaccgccg
      481 ccggcaggct caggccggcc agcacgccgc cgacaatggt gaccaggctg cggctgactc
      541 ccggccacat ggccaagcat tggccgaggc cgatggccac ggccatgggg atcgtgagct
      601 gctcgagcgc cagacccggt ttggccggcc cgttcgagga acggcgcatc caggcgaccg
      661 ccagaatcgc gactccgccc acaaaccagg ccgccactac gggccacagg ccccaggtat
      721 cgccgccaaa gaggtatttc ttgatgagct tctcgaggag gaacccgatc accgccgccg
      781 gcagaaaggc gaggaggata ttccaaccaa gcttccaacc cgccggatcc tgtcccagcg
      841 cgccgcgggc catttgcagc acgcgtttgt aatacagtcc gagcaccgcg aggatcgcgc
      901 cgacctgaat gcagatggca taagcgtccg cggcgtcgct ggcggaaatg cccagcaggc
      961 gctgggttaa aagcaaatgc ccggtcgaac tgacgggaag atattccgtc aggccctcaa
     1021 cgaggcccaa aatgagggat tgccaccact ccatgaatca actcggcggc ggcaagccgc
     1081 tggacaaatc aattaaatcg cgttttcgcc ggtttcttcc gtgcgaatgc gaacggcgtg
     1141 ctcaatcggc agaatgaaca ccttgccgtc gccaatcttg cccgtctttg ccgcggtgga
     1201 aatggcggcg acggtttttt ccacaaggcc atcggccacg acgatttcaa atttgacctt
     1261 cgggagaaaa tccaccgtgt attcgcttcc gcgatagatt tccgtgtggc ccttctgacg
     1321 gccaaatccc ttgacctcgc tcacggtcat tccctcgatg ccgatttcag ccaaggcctc
     1381 tttgacatct tccatcttga acggtttaat gatggcttcg atttttttca tagcagcagc
     1441 tttgttttaa aagggactat tcattttgca agcgcagaag caagcagata aggccgttga
     1501 gccgggatta aatgctcatt ggaggcggga tgcgtcccat tgagtggcgg cggacgcgcg
     1561 tggggcgtta cctccgccat ctgccgcggc cgaaacacct gcgcggaagc tggctgcacc
     1621 ggaaactggg ggacagcctg ctggcgcgcg acctgtggca tcccgacccc tcgcgcgtgg
     1681 cggccggctt ttcactgggc gcgtttttcg cggtcatgcc cattcccttt caatccattc
     1741 ccgccgcgct gctggcgttt ctgacgcgtt ccaacatcgc cgccgccctg tgcgcggtct
     1801 ggattacgaa cccctttacc gcgccttttt tcatctacgc gcaataccgg atcggcgcct
     1861 ggatcctgcg ggatccgaat ccgattcgct tccaattcca gcaggccatc gattggaatc
     1921 agttcttcag cgaagcgccc aaggccttcc tggcgggctc ggtcgccgtg gggttgattt
     1981 cggcggcggt cgtttatccg atcgtgctcg tggcctggaa gggattggcc ctctgggtgc
     2041 ggtccgcgca ccgcaagcac ccttcggcgg gaaccggcga cgattgaatt gattccgcgc
     2101 ccggttttgt gcgactattt tgactccaaa cctgacgcat tgaatgaaat tcctcctcct
     2161 gccgctggtg ctggctgcct gcctcgcgtc cgctcacgcg gaacgcctga ttccgagttc
     2221 ggtggatcca gcctccgccg cggcgcggga aaaggcgttc attccctggt ccactcccga
     2281 ggaatcgtcc gccttttgga agaagttgtc gccgaaccat gtgccggtct attacgaacg
     2341 caaggacggg ccgctcagcc gggatattta cattccgaat ccgggcatcg gctattgggt
     2401 gctggggggc ctgacggaaa agcagctgtt caagattcac cgggaaaagc tggaaatcga
     2461 cgacacgctg atcagcgcct cggcgtatcc ggatgagcgg ggaaaccgcc tttactgggc
     2521 gttgtgggcg ccgagggagc gggcgtatct cctaacggat cccatgaaaa aactcggcat
     2581 cggccaggcc cgcatcgagt tttcctgggc ggatcggttt ctcctttggg cggcggagat
     2641 gaagcccttc tccggattca tcacggcggc ctcgctggcg ttgaatggac tgctgtttct
     2701 gctggtgggg gcaatcgtcc ttggcggcaa ggcgtcccgg atcaactcct cctctccggg
     2761 ttaatcgtcg ttgcgcggag gacgccgaat gcgcatttcc cgattgccgt ttcgcgcgga
     2821 tccaggcaag tttagcgcct gatgatccgg cgccttcttc ccatcctcct cctgctggcc
     2881 ggaaattgcg cggcgcccgc ggcggcttcc aaaccgctgg gggaggccgc cgccgggctg
     2941 gcggctcaat tgccaaaagg cggcatcgtc accggcgaat cggcgggcgg cccggcggcc
     3001 tatgccctgg ccgggaatgt cggggtggcc ggcgttccgc cggaacggat cctttttgag
     3061 atcggctcca tttccaaggt ttttaccggc ctgctgctgg cccaggccgt cgtcgaaggc
     3121 cgcgtcaagc tggatgcgcc catccgcgat ctcctggagc cgaaacaaac ctttgccgat
     3181 gcccgcgtgg gagcgattac cctccggcag ctcgccgcgc acaccagcgg gctgccgcgc
     3241 ctgcccgagg agcttgacca gggggcgaat cccggcgatc cctacgcgca ttacgaccgc
     3301 gcccggttgg aggccggcct ggcccggctc caactcgccg gcgacgcgcc ctttccccgg
     3361 agttattcca actacggcgc gggcctgctg ggcgacctgc tttcgcgcct ctacggaaag
     3421 ccctgggaaa ccctggttcg cgaaaaaatc gcggggccgc tcggcctgcc cgacaccgtg
     3481 gtccggctgg atgcggatca gcaaaggcgc ttcgcgccgc cgttcagcgg caattccccc
     3541 gcgcatccct ggaatctcgc ttcgctcgaa ggcgcgggcg cgcttcgctc caccgcggcg
     3601 gatttgatgg ccttcggcgc ggcgctgctc catcccgggc aaacgccgct tcgtgaagcc
     3661 ctcgagctcc tgcttgctcc gcaaagcgcg gatggtgcca tcggcctggg catctccctt
     3721 tcgcgcttgt tcggcgagcg cggctacgag cacagcggcg gcaccggcgg gtttgccagc
     3781 ggcctgtatg tgctgccgga gagcgggtcc gtgaaagtca tcctgatcaa caacgcggtg
     3841 ctttccgatg gagccgtcct cgccgccacc cactcctccg cgccgccccg ggaatccgcc
     3901 cgcgcgtttg ccgcggacga attggccgaa ttcaccggcg tgtatccgct ggattccatg
     3961 agccgcttca cgctgctgct tcgcggcgga cagctctggg tccggctcag cgggcagccc
     4021 ttttttccct tgcatccgca cgcgactccc gatcgctttt ttttgaaggt cgtgccggcg
     4081 gaaatccagt tcgagcggaa ggagggggca atccaatccc tcacgctttt tcagaatggc
     4141 cgggagaccc aagccgcgaa aagtccggaa ccggcgccgg tgatcgattt ccgctccgac
     4201 cgggaactcg cttccctggc cggagcctac gccgtgaatc cggagatgaa tttcacggtg
     4261 aaagtccgtc attccacgct tttcgtgaaa ctcaaccagc agcccgccct gccggtcttt
     4321 gaaacaagca aggattggtt tgaatacgat gtcgtctccg ccgcgctgga attcgagcgc
     4381 gacaaggaaa accgcgtcgt cgcgctctgg ctttttcaga acggcgcggc tttccgcgcg
     4441 gcgaagaaag attagcgcat tttcggttta attggacgcg ttcctcccgg cggcgttagc
     4501 aacgccgctc caccgaaaga tccgtccggc ggcagggcgg tgtcgccgac accgccggtc
     4561 acggcgacaa aataaaggga aaaacccgct gccgcgcggg gttgcggagt cagccggaat
     4621 gcgagccgat ctccagcgag tccgccttcg attttccgat cgcgacgctg acatacagca
     4681 ccagcgaaca gccaatcggg aagatgatct cattcacgag gcgggccatg gtttccggcg
     4741 gagacagcgg cgccttcgtc aaccaaacat aggtcccctg gaccatcagg ccgaagatca
     4801 agccgagccc gaacccggtt cccgcgtaag cccaggggga ttgaacggac tttctgacca
     4861 ggaggcagag cgccaggccg agcagtccgt attccaccgc gcggatggcc gagacggcca
     4921 gcgtgccggc gagcgtgccg ggggcggcca gattcatcgc ttccagaatg gatttgtgcg
     4981 tcacccgggc caggatcgtg gcgatgggcg cggagatcag gccaaagagc agcatgggaa
     5041 gcgccgcggt gcgcgtgacg atgatcccga ccgccagccc gatgcagatg aggacggacc
     5101 acgtgatctt tcccacgacg tcggcggcga accggccggc cgcggtgtca atccccaggg
     5161 aaaccttggc cgccaggagg atcgcctcga ctcccaaacc cagcagcacg gccagccaag
     5221 ccaccttcag gatggcccta aaaaccttcc cctggctcgg taacgtgtcg tccgcttgtt
     5281 cagcgttttc cacagcagga atcgcgtaac aaaacccgcc gggattggca agaccgattc
     5341 aagggaaccg ggaaatcggg gagtgttcaa aaaatgaacc gtgcggggtg cgaaaagccc
     5401 gcccggcggc ggcttcccaa cggatttgct tgccgcggga ccttgccgaa cctagtcaag
     5461 ggagaaccct tttagggggt gtagctcaac ggtcagagca ggcggctcat aaccgcttgg
     5521 ttgccggttc gaatccggcc gcccccatta tcacccctat taccaagaaa acagcgagaa
     5581 tgaatgtatt gatggccgca agcgagatgg cgccctttgc gcggacgggg gctttggcgg
     5641 atggaatggc ggccctgccc agcgcgctcc ggcaggcggg acacgatgtc cgcgtggttt
     5701 tgccctatta ccgaaccatt cgggatggac gggacttctc ggtccggccc accaaaatcc
     5761 ggtttgccgt tccggtgggg gaggcgcggt atgattgcga gatttacgag gcgcgcgcgg
     5821 ccgacggcgt gcaggtgttt tttatcgggc gggatgaatt cttcgatcgc agcggcctct
     5881 acggatccag cggccgggat tatcaggaca atgccgcgcg cttcatcttt ttcaacaaat
     5941 gcgtgatcga gctggccaag cggctcaccc cggttccgga tattcttcac gcccatgact
     6001 ggcaggcggc gctgctgccc ctgctggcga agtcccagag cctgccctgg ccggtggtgc
     6061 tcagcgtgca cgacccggcg tatcagggaa atttctggag ctacgatttc gggctgacga
     6121 atctgccgcc ggattatttt tcggcgcggg gtttggagtt ttatggaagc atgaatttcc
     6181 tcaagggcgg gattttgttc gccgaccgcg tggtgctgcc cagcgaactg tatgtcagcg
     6241 aggcgcagac ccgcgaattt ggctgcgggc tggacgccgt tttccgggag aacgcgggca
     6301 agctggcggg cattcccgat ggcgtggacg tgacccaatg ggatccggct tcgggccggc
     6361 atctggcggc gccgtattcc gccgccggcc tgggcggcaa acgcgcctgc cgggatcagc
     6421 tcctcgccca attctccctc caggccaatc cgaaggggcc ggttttcgcg atggtctcgc
     6481 ggctcgtgca ggagaagggg ctggatattt tgctgccggc gctcgaccgg
//
LOCUS       DATDAY010000181         4198 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_1420494, whole genome shotgun sequence.
ACCESSION   DATDAY010000181 DATDAY010000000
VERSION     DATDAY010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 4198)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4198)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4198
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1420494"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(61..2856)
                     /locus_tag="VIS74_03560"
     CDS             complement(61..2856)
                     /locus_tag="VIS74_03560"
                     /inference="COORDINATES: protein motif:HMM:NF024240.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY5792348.1"
                     /translation="MLFILSLLVSEPLSAATAGLAGNPPVITSPPLANGYAGLPFKYR
                     ITATNAPTLFLASGLPASLQINGRTGLISGTLANSGTYSIVIAAKNSSGTAKKWVNLF
                     IRTPTVVVKTQKGRPVLTVPVLCDAHGRTLYTYQPDAATPATAPNLPDAAPWQWARIS
                     DKPVAGPGINATLGSKIKDGKTILTINGLPAYYHTRESAGETTAWNLPDWTVFNADGL
                     PMEKAEVIVYGGTPSGIMAAIAAARHGHSVALIDINKHLGGVVSGGLTETDIGDSKTV
                     GGVVTDFFARIKRHYVLTYGANSPQDRKSHNGRNFEPHVAEAIFEQMLREWPNIRIWR
                     RHRYQSLTLHQGNIESLTVEHASLKFTRTFVGDLFIDASYEGDVMAGAKVPYRVGREA
                     RAEFGEYLAGVQAGPEESIGSADHRVMAYNYRVTLTPFTPNRVLFPKPANYDPEPWRP
                     TVGNRILKNGFKHFADLYNDAAAREDPNYKLDTNWGNLVGGNVAYPDGDWRTRDRIAA
                     RHRDHFLSLLYYLQNDPELPADFRADARKWGLPADEFTDNGHFPFQMYIREARRMVGR
                     YVLRERDLTQSRFKTDGVCSGSYGIDCHWIRIIYDEKGRRFADITPHLDVSCYDIPYA
                     SLTPENPANLLVSVCISATHVAYCSLRMEPVYMMLGQAAGSAAHLALESHTTVQEVNV
                     NQLRRLLRKEGAILDSGYMPAVHVTWTPARPKPGEKVTFQLAPGMIKDPLNQVWWDFE
                     GAGKVSAKGPSASHTFELGKLYQPNVLVTDAAGRRRLISVQVPVGAASRSDFTVDDFD
                     ATLSGKWNATYPKTPLLNGLLDPDIFFGPGIHRSDGKVSALFQPVIPRTGRYKVCVGF
                     RPAADQATNMPVTIRHAGGDTTIIVNERQETTPFNFTPIGTFTFNAGSNGYVKITNDG
                     ANGLVAIDGVRWIWLGE"
     gene            complement(3128..>4198)
                     /locus_tag="VIS74_03565"
     CDS             complement(3128..>4198)
                     /locus_tag="VIS74_03565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792349.1"
                     /translation="NIGINGLAPVFEPNNPDLVPVWHPWPGESVNLAISRPEAIAGAT
                     VTVNRASHEITLGKRQRLSKLDLSVQSSLGEDFLVELPAAAEITAVRLNQQALPVRKD
                     GPRVIIPLRPGSQNVAIDWKMDTPLGFRTAAEAVRLPVESANVNTTLRLPDDRWVLWT
                     FGPLRGPAVQFWVVLACSLIAAGLLGRLRHSPLNPFQWMLLALGLTQVPLPAAIVIIG
                     WLFFTQWRGRDSFQRLPGWGYNLFQLFFAGLTVAAAGVFIAVVAAGLLGDPEMFIRGN
                     GSSYTALKWYQARSGVDLPTPVCFSVSIWWYRLLMLVWALWLAASLIRWLAWAWRQFG
                     AGGYLRKSPPKVVLPPRVPPGA"
BASE COUNT          811 a         1176 c         1382 g          829 t
ORIGIN      
        1 gcctatgctt gcgctttgtc ctctggattt taaagcgaat tttccgggca gggagaagtt
       61 ttactctccc agccaaatcc agcgcacgcc gtcgatggcg accaggccgt tggcgccatc
      121 gttggtgatc ttcacatatc cgttgctgcc cgcattgaag gtaaacgtgc cgatgggcgt
      181 gaaattgaag ggggtggttt cctggcgttc gttgacgata atcgttgtgt ctccgccggc
      241 gtggcggatg gtcaccggca tattcgtcgc ctgatcggct gcgggccgga agcccacgca
      301 gaccttgtaa cggccggtgc gcgggatgac cggttggaac aaagcggaaa ccttcccgtc
      361 gctacggtga atgccgggac cgaagaaaat gtccggatca agcaggccat ttaaaagagg
      421 ggttttagga tacgtggcat tccattttcc ggaaagcgtg gcatcaaagt cgtccaccgt
      481 gaaatcgctt ctggaggcgg cccccaccgg cacttgcacc gagatcaatc ggcgccggcc
      541 ggcggcatcc gtgacaagca cgttgggttg gtatagcttt ccgagttcga aggtatggct
      601 ggccgacggg cctttggcgg atactttgcc ggcgccctca aaatcccacc aaacttgatt
      661 aagcggatct ttaatcatcc cgggagccag ctggaaggtg actttttcac ccggtttggg
      721 ccgggccgga gtccaggtga catggacagc cggcatataa cccgagtcca gaatcgcgcc
      781 ttccttccgg aggaggcggc gaagctgatt cacattgacc tcctgcacgg tggtatggga
      841 ctccagcgca aggtgggcgg cgctgccggc cgcctggccc aacatcatgt aaacgggttc
      901 catccgcagg gaacagtagg ccacgtgcgt ggcggagatg cagaccgaca cgagcaggtt
      961 cgccggattc tccggggtga gggaggcgta gggaatgtcg tagcagctga cgtcgagatg
     1021 gggcgtgatg tcagcgaatc gccgtccttt ttcatcatag ataatccgga tccagtgaca
     1081 gtcgatgcca tagctgccgc tgcatacgcc gtctgtcttg aaacggcttt gggtcagatc
     1141 gcgttcgcgc aaaacataac ggccgaccat tcgccgcgct tcacggatat acatctggaa
     1201 agggaaatgg ccgttgtcgg tgaactcgtc ggccggcagc ccccacttcc tggcatcggc
     1261 gcggaaatcg gcgggcagct ccgggtcgtt ttgcagataa taaagcaggc tgagaaagtg
     1321 atcccgatga cgcgccgcga tacggtcgcg cgtcctccaa tctccatccg gatatgcgac
     1381 gttgccaccg accaggttgc cccaattggt atcgagctta tagttgggat cctcgcgcgc
     1441 ggcggcatca ttgtaaagat ccgcaaagtg cttgaagcca tttttcagga ttctattccc
     1501 cacggttgga cgccagggtt cgggatcata gttcgcgggt ttgggaaaga gcacccggtt
     1561 gggggtgaat ggagtgaggg tgacgcggta attataagcc atcacgcggt ggtcggcgct
     1621 gccgatggat tcctccggtc cggcctggac gccggccaga tattcgccga attccgcccg
     1681 cgcttcccgg cccacgcgat agggcacctt ggctccggcc atcacgtcgc cctcataact
     1741 ggcgtcaata aagaggtctc ccacaaacgt gcgggtgaat ttcaggctgg catgctcgac
     1801 cgtgagcgac tcgatgttgc cctgatgaag cgtcagggat tggtagcgat gccggcgcca
     1861 gattcggatg ttcggccatt cccggagcat ttgctcgaaa atggcttccg ccacgtgggg
     1921 ttcaaaattc cggccgttat ggcttttccg atcctgcggc gagttggctc cgtaagtaag
     1981 aacgtagtgc cttttgatgc gggcaaagaa atcggtcacc actccgccga ccgttttgga
     2041 atcgccgatg tccgtttccg tcaacccgcc gctcaccacg ccgccgaggt gtttattgat
     2101 gtcgatgagc gcgaccgaat gcccatgccg ggcggcggcg atggctgcca taatgccgga
     2161 gggggtgccg ccatagacga tgacttcggc cttttccatg ggcaggccgt ccgcattgaa
     2221 aaccgtccaa tcgggcagat tccaggcggt ggtttccccg gcggattccc gggtatgata
     2281 ataagcgggc agtccattga tcgtcaggat ggttttgcca tctttgatct tggaacccag
     2341 agtggcgttg attccgggac ccgccaccgg tttgtcggaa atccttgccc attgccaggg
     2401 ggcggcatcc gggaggttcg gcgcggtcgc cggggtggcc gcatcgggct ggtaggtata
     2461 aagggtccgg ccgtgggcat cgcagagaac cggaacggtc aaaacgggcc gccccttttg
     2521 agttttgacc acaacggtgg gggtgcgaat gaagagattc acccactttt tggcagttcc
     2581 gctgctattt ttggcggcaa tgacgattga gtaagtgccg ctgttcgcca gggtgccgga
     2641 aattaggccg gtccggccat tgatttgcag gctggcggga agtccggaag ccaggaagag
     2701 agtcggggca ttggtcgcgg tgattcgata cttgaatggc aatccggcgt agccattggc
     2761 gaggggcgga ctggtgatga ctggcgggtt ccccgccagg ccagccgttg cggcggacaa
     2821 cggttccgaa acaagcaggc tcaagatgaa aagcattccc cggtattttg gaaagaggcg
     2881 aatcacgttg agtaattgat gagcctcacc ccatcagcgg gaccgccgtt gtcaatattt
     2941 gagtcgaaaa acttcaatcc gtttgcgaat gagtccggct gcgagtggca tcggcatcct
     3001 gccgatgcgt ggagtttgga tcatcggcag gatgccgatg ccactggcgc ttggcaccag
     3061 cgtctccccc gcgggttgag tttttcgttc ttttctgaat cggccgcgtt tctttttggg
     3121 aaatgcgtca agcccccggc ggcacgcgcg ggggcaggac gactttcggc ggggatttcc
     3181 gcaggtaacc gccggcgccg aactgccgcc aggcccaggc cagccagcgg atcagcgacg
     3241 ccgccagcca gagcgcccag acgagcatga ggaggcgata ccaccagatc gagacggaga
     3301 agcacacggg cgtgggcaga tcgaccccgc tccgggcttg ataccatttt agcgcggtgt
     3361 agctggagcc gtttccgcgg atgaacatct cgggatcgcc gagcaggccc gcggcgacga
     3421 cggcgatgaa gactccggcg gcggcaacgg tgaggccggc gaaaaagagc tggaagaggt
     3481 tgtatcccca acccggcaga cgctggaagg aatcccgccc gcgccattgg gtgaagaaca
     3541 gccagccgat gatgacgatg gcggcgggca gcggcacctg ggtgagcccg agggcgagca
     3601 gcatccattg aaacggattc agcggcgaat ggcgcaggcg gcccaggagc ccggcggcga
     3661 tcagcgagca ggccagcacg acccagaatt gcaccgcggg cccccgcagc gggccgaagg
     3721 tccaaagcac ccagcggtcg tcgggcaggc gcagggtggt gttcacgttc gcgctttcca
     3781 cgggcaggcg cacggcttcg gcggcggtgc gaaacccgag cggcgtgtcc attttccaat
     3841 caatcgccac attctgcgag ccggggcgca ggggaatgat cacccgcggc ccgtccttgc
     3901 gaacgggcag ggcttgctgg ttaaggcgga cggcggtgat ttccgccgcg gcgggcagct
     3961 ccaccaggaa gtcctcgccg aggctggact gcacggagag atcgagcttc gagaggcgct
     4021 ggcgtttgcc gagggtgatt tcgtggctgg cgcggttaac cgtcactgtc gcgccggcga
     4081 tcgcttcggg ccggctgatg gcgagattca cgctttcgcc cggccaggga tgccagacgg
     4141 gaacgaggtc gggattgttc ggttcgaaga ccggggccaa gccgttgatg ccgatatt
//
LOCUS       DATDAY010000182         4083 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_1280194, whole genome shotgun sequence.
ACCESSION   DATDAY010000182 DATDAY010000000
VERSION     DATDAY010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 4083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4083
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1280194"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..208
                     /locus_tag="VIS74_03570"
     CDS             <1..208
                     /locus_tag="VIS74_03570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792350.1"
                     /translation="EFLAVLRRQPEYAQIPVVVVTAKDLTDEDRLRLQGSVQDVMQKG
                     AMDRERLLNEVIELIAKTHTEPAK"
     gene            205..591
                     /locus_tag="VIS74_03575"
     CDS             205..591
                     /locus_tag="VIS74_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006983980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY5792351.1"
                     /translation="MIRILLVEDNEMNRDMLSRRLQRKNFEVEVAVDGREGLDKARAS
                     IPDLILMDMSLPVMDGWEATRQIKADEAVKRIPVIALTAHAMAGDREQALGAGCDDYD
                     TKPIEFPRLLSKIEAQLARIGKTPAQ"
     gene            705..2579
                     /locus_tag="VIS74_03580"
     CDS             705..2579
                     /locus_tag="VIS74_03580"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HEY5792352.1"
                     /translation="MADSSKETPLAKFRHDLRTPINHILGYSELIHEELADAGNTGHS
                     EDLLKIRGAAQSLLEQIGQNLTPDGIQRLLAGEPAAAVAAPSGEKPALANDAGWVDAE
                     AAIRGRILVVDDNAENRETLARRLRRQGHEARESPDGRHALELLRSESFDLVLLDVIM
                     PEVDGYTVLREIKADEALRDLPVIMISALDELDVVVRCIQAGAEDYLPKPFEPTLLRA
                     RIGAGLEKKFLHDQEKQYLERIEETQQRLNAELDEAANYVRSILPAPMTEPFRIDWRF
                     IPSTELGGDSFGYHWLDGEHFAIYLLDVCGHGVGAALLSVTAINVLRSGALGDADFHD
                     PARVLGALNETFPMEKQNNMYFTMWYGVYHVPTRTLRHASGGHPPALLVTGDGEARPI
                     ASQGTLIGVLPGMTFKSATDVIPEGGRLFLLSDGTYEVKNPDGSMLDYEVFRKFMGDN
                     GGAPDALDRLTGWIRSLNGEGPLDDDFSIVSFDLAPGAKIEFQIRNHFDELPALSEKV
                     TGFLQSHGAGGEAEFAANLAIEELVTNLVKYGYDDQKEHAICIRLSLEKDALHLEIVD
                     DGHEFNPFELPEPDTSLPAEEREIGGLGIHFVRHLLDHCEYRRENGMNRLSLRKNLTR
                     "
     gene            complement(2756..3301)
                     /locus_tag="VIS74_03585"
     CDS             complement(2756..3301)
                     /locus_tag="VIS74_03585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792353.1"
                     /translation="MKRLLAIFSFFLAATWAVAQPVLIVVAEGKQVALSPADAARLIL
                     KIEDIVVSANFNSRDHPADYFKEGDYWRPISKIQKGSYIRITYPEGHVFKTAGGEISA
                     KDVWIDLPHEARSSGGAVYPGPITLINGEDRILLTKESGYLVLGLGLDPAIYPHLPKI
                     LQENMDRGRKAYEEFEKKKNG"
     gene            complement(3390..3593)
                     /locus_tag="VIS74_03590"
     CDS             complement(3390..3593)
                     /locus_tag="VIS74_03590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HEY5792354.1"
                     /translation="MTLTAVLTPDPDGGYVAHNPETGTTTQGDSVSEALANLKEATEL
                     YLEEFPLDSTSPSLMTTFEVAHA"
     gene            complement(3743..>4083)
                     /locus_tag="VIS74_03595"
     CDS             complement(3743..>4083)
                     /locus_tag="VIS74_03595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792355.1"
                     /translation="RSEGKTNYRRTFTPPLIRFSDVIFYHFIHLGGAIITDIFTTSLK
                     EALAEHQIVLASLNAQHGGIPLHEKDEDALISKIESESYSTWMIESAIGFSGFVVGKA
                     ITGEETEQGA"
BASE COUNT          875 a         1290 c         1129 g          789 t
ORIGIN      
        1 tgaatttctc gccgtgctcc gccgccagcc ggaatacgcc caaattcccg tcgtcgtcgt
       61 gaccgcgaag gatctgaccg acgaagaccg cctgcggctg caaggcagcg tccaggacgt
      121 catgcaaaaa ggcgccatgg accgcgagcg gctcctgaac gaagtgattg aattgattgc
      181 caaaacccac accgaacccg ccaaatgatc cgcatcctcc tggtcgaaga caatgaaatg
      241 aaccgcgaca tgctcagccg ccggttgcag cgcaaaaatt tcgaggtgga ggtcgccgtg
      301 gacggccggg agggcctgga caaggcccgc gcgtccattc ccgatctcat tctcatggac
      361 atgagcctgc ccgtcatgga cggctgggag gccacccggc agatcaaggc ggacgaggcg
      421 gtgaagcgga ttcccgtcat cgcgctcacc gcgcacgcca tggccgggga ccgcgagcag
      481 gcgctcggcg ccggctgcga cgactacgac acgaagccga tcgaatttcc gcgcctgctt
      541 tccaagatcg aagcccaact cgcgcggatc ggaaaaactc ccgcgcaatg acctcccgcg
      601 cttcatcatt actccaggca gggcagccgc gccgcggctg cccattttgg atcgaaacaa
      661 gccggcaccc gcggcgcggc tgccctgcct ttcatcatta agggatggcc gactcctcca
      721 aggaaacccc gctggcgaag ttccgccacg atctgcgcac gccgatcaac cacatcctcg
      781 gctactcgga actcatccat gaggagctcg ccgacgccgg gaataccggg cattccgaag
      841 atctgctcaa gatccgcggc gccgcccagt ccctgctgga gcagatcggg caaaatctca
      901 cgccggatgg gattcagcgc ctgctggccg gcgaaccggc ggcggccgtg gccgcgccgt
      961 ccggcgagaa acccgcgctc gcgaatgacg cgggctgggt ggacgccgaa gcggccatcc
     1021 ggggccggat cctcgtcgtg gacgacaacg cggagaaccg cgaaaccctc gcccgccgcc
     1081 tccgccgcca ggggcatgag gccagggagt cgccggacgg caggcacgcg ctcgaactcc
     1141 tgcggagcga atccttcgat ctcgtgctgc tggatgtcat catgcccgaa gtggatggct
     1201 acacggtcct gcgcgagatc aaggcggatg aagccctgcg cgatctgccc gtcatcatga
     1261 tctccgcgct cgatgagctc gatgtcgtcg tgcgctgcat ccaggccggc gccgaggatt
     1321 acctgcccaa gcccttcgag cccacgctgc tccgcgcgcg catcggcgcc gggctcgaaa
     1381 agaaattcct ccacgaccag gaaaaacaat acctcgagcg gatcgaggaa acgcagcagc
     1441 gcctcaatgc cgaactcgac gaggcggcga attacgtgcg ctccattctg cccgcgccca
     1501 tgaccgagcc cttccgcatc gactggcgct tcattccctc caccgagctc ggcggcgact
     1561 catttggcta tcactggctc gacggcgagc acttcgccat ttacctgctc gacgtctgcg
     1621 gccacggcgt cggcgccgcg ctgctttccg tgaccgccat caatgtcctg cgctccggcg
     1681 cgctgggcga tgccgacttt cacgaccccg cccgcgtgct cggcgcgctg aacgagacct
     1741 tccccatgga gaagcagaac aacatgtatt tcaccatgtg gtatggcgtg tatcacgtcc
     1801 ccacgcgaac cctccgccac gccagcggcg gccatccgcc cgcgctcctc gtcaccgggg
     1861 atggcgaagc ccgcccgatc gccagccagg gcacgctcat cggcgtcctg cccggcatga
     1921 cctttaaaag cgccacggac gtcattcccg aaggcggccg ccttttcctg ctcagcgacg
     1981 gcacctacga ggtgaaaaac ccggacggct ccatgctcga ttacgaggtc ttccgcaaat
     2041 tcatggggga caatggcggc gcgcccgacg cgctcgaccg cctcaccggc tggattcgct
     2101 cgctgaatgg cgaaggcccg ctcgacgacg atttttccat cgtttccttc gacctcgcgc
     2161 ccggcgcgaa gatcgaattc caaatccgca atcacttcga cgaattgccc gcgctctccg
     2221 agaaggtgac cggcttcctc caaagccacg gagccggcgg cgaggcggaa ttcgccgcga
     2281 atctcgccat cgaggaactc gtcaccaacc tcgtcaaata cggctacgac gaccagaagg
     2341 agcacgccat ttgcatccgg ctgagcctgg aaaaggacgc gctgcatttg gaaatcgtgg
     2401 acgacggcca cgagtttaac cccttcgagc ttcccgagcc ggatacctcg ctgcccgccg
     2461 aggagcgcga gatcggcggc ctcggcatcc acttcgtccg ccacctgctc gaccactgcg
     2521 aataccgccg cgaaaacggg atgaaccggc tatcgctgcg gaaaaatctc actcgctgag
     2581 ctcgtccggc ccttttagag cgatcctcaa aagagatttc cgaaaagcga ttcactacag
     2641 gggcggttgt gccaaccgcc ccgcgtaaac ggggatgcgg ggtccccatt tccccgcaaa
     2701 caaaggcgcc caatggggcg gtggagcccc atttccccac ccggcaagcg ccttgttagc
     2761 catttttctt cttctcaaat tcttcataag ccttccgtcc tctatccata ttctcttgga
     2821 gaatcttggg aagatgcgga taaatcgccg ggtcaagtcc caaacctaaa actaaatatc
     2881 cactctcctt cgtcagtaat atcctgtctt caccgtttat cagtgtgatc ggaccaggat
     2941 atacggcacc accggatgac cttgcttcat gcggtaaatc aatccataca tcctttgcgg
     3001 agatctctcc tccggccgtt ttgaacacat gaccttccgg ataagtgatc cgaatatatg
     3061 atcctttttg aattttggaa attggccgcc aatagtctcc ttccttgaaa taatccgcgg
     3121 ggtgatccct cgaattaaag ttggctgaaa cgacaatatc ttcaatcttc aggataaggc
     3181 gggcagcatc agcaggcgaa agcgcaacct gcttgccctc ggctacaacg atcaatacag
     3241 gctgcgccac agcccacgtc gctgcaagaa agaagctgaa aattgcgagg aggcgtttca
     3301 tttctggcta acgtccttcg tcagccgcgc cgctttgcgg cccattttct gaagggcgga
     3361 gaggcactct actgccaatc ctggggagtt taggcatggg caacctcgaa agtggtcatc
     3421 agagacgggg aggtggaatc caaaggaaat tcctccaaat agagttctgt ggcctctttc
     3481 aggttggcta aagcttccga gacagaatca ccttgggtgg tcgtgcctgt ttctggattg
     3541 tgggccacgt agcctccatc cggatcggga gtcaatacgg cggtgagtgt cataaaagca
     3601 acttacgcgg gtgtgggcag aacggcaatt ttttccgaac gtgtaagctg agctatggcg
     3661 aacggggcct cacttcaggg ggaaacgggg aaagcaggcg ggaagtcgga acgtagtcca
     3721 cggaacgaat gtagccatta gctcaagcgc cttgttcggt ctcttctccg gtgattgctt
     3781 ttccgactac gaacccagaa aagccaatcg ctgattcaat catccaagta gaatagctct
     3841 cggattcgat ctttgaaatg agcgcatctt catctttctc gtgaaggggg attccaccat
     3901 gctgtgcatt caagctcgcc aaaacgattt gatgttctgc taatgcctct ttcagtgaag
     3961 ttgtaaaaat atctgtgata attgcgccac ctaaatggat gaaatggtaa aaaatgacgt
     4021 cgctgaatcg gatcaatggg ggtgtaaaag ttcttctgta attggtcttt ccctctgagc
     4081 gcc
//
LOCUS       DATDAY010000183         3550 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_2131657, whole genome shotgun sequence.
ACCESSION   DATDAY010000183 DATDAY010000000
VERSION     DATDAY010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 3550)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3550)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3550
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2131657"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            39..2774
                     /gene="glnD"
                     /locus_tag="VIS74_03600"
     CDS             39..2774
                     /gene="glnD"
                     /locus_tag="VIS74_03600"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: protein motif:HMM:TIGR01693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0018177 - protein uridylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HEY5792356.1"
                     /translation="MSITRDPIFDQAVAALTEQARKKQGSLIEIFKRFRKIQEHRLKI
                     WHNAGGGGREIARRRSDLVDVLFRELFEQIVSEVRPKASIEDSLTVVAFGGYGRREMN
                     PFSDVDIMFLTAKSNPGKTMEELIRRVMMALWDLGYKVGHSVRSIAQAIQQANADMLT
                     KTSMLECRHLLGSRELFNEFKDRFEQSCVQGHEAAYIEWRLTNMAELREKYGASVFMQ
                     EPNVKCGCGGLREYQNLLWISNVKEHAPTTAKLVEKKILRETERRHLEQGYDFLLRVR
                     TEMHYLNERPIDILTLRLQGLVATAFGYPQRNILRRCEAFMRDYYQHTRHVQLIAQAA
                     IARLEVQPEPTGASLLQMLHLKKTRAEKFDGFVAKDGYLYPVSRDIFNEDTARLMRVF
                     QHVQRRQLQLSPELRDLILRRTRLMDRTFQYARVNREVFLSILSHKGRVGPILREMHE
                     LGFLGKYLPEFGALTCLVQHEFFHRYTADEHTLVCIEKLDNILFSEDEKLRGYRALFQ
                     KQDDPAMLYLALLLHDTGKAANTRNHEEASAELAHRVARRLQLTPQRRRMLITLVDVH
                     YSLSQISQQRNLDDLTTITTFAGIVKNREILDALMLITLADGMGTSDEKWSDWKEALV
                     WQLYRRTVAYLEHGPAAFDLKQKNLTEIRSALRLPKAMEEEVEAHFQHMPGRYFRSFN
                     ADQVRQHLRLFHTFFERRAGGEDPIKPAVEWIQHPGQGHTEVCICGWDRHALLEKIAG
                     AFLSAEINILSADVFTRGDSLVLDLFRVCDTRHQPVTSPKDFARVESRLNEALRHEDY
                     DFTPLLAREARMKFYRLSQDAELPTRIVIENDAHPIYSIIEIQTPDRLGLLYDLLRAF
                     GAADVHIELSRITTEMDVAMDSFYVTELDGRKLTDKAAIARLQELLLKASARKRA"
     gene            complement(3352..>3550)
                     /locus_tag="VIS74_03605"
     CDS             complement(3352..>3550)
                     /locus_tag="VIS74_03605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792357.1"
                     /translation="SVACGFYYYLKIVRAMYWMPVGDSPERSAPIPVTRLTRLALVGL
                     IAATLVLGVYPEWILRVFSRF"
BASE COUNT          733 a         1180 c         1014 g          623 t
ORIGIN      
        1 ggtggaaatc gtgattcagg acgaataatc ccaccgcgat gtccatcaca cgggatccca
       61 ttttcgacca ggccgtcgcc gcgctcacgg aacaggcccg caagaaacag ggcagcctca
      121 tcgagatctt caagcgattc cggaaaatcc aggagcaccg cctcaaaatc tggcacaacg
      181 ccggcggcgg gggccgggag atcgcccggc gccgctcgga tctcgtggat gtgcttttcc
      241 gcgaactctt cgaacagatc gtcagcgaag tccggccgaa ggcttccatc gaggattctc
      301 tcaccgtggt ggcgttcggc ggatacgggc gccgggaaat gaatcccttc agcgatgtgg
      361 acatcatgtt cctcaccgcc aagtcgaacc cgggaaaaac catggaggag ttgatccgcc
      421 gggtgatgat ggcgctctgg gatctcggtt acaaggtcgg ccattccgtc cggtccatcg
      481 cccaggccat tcagcaggcc aacgccgaca tgctgacgaa gacctcgatg ctggaatgcc
      541 gccacctcct cggaagccgg gagctgttca acgagttcaa ggaccggttc gagcagagct
      601 gcgttcaggg gcacgaggcg gcctacatcg aatggcggct gaccaacatg gccgaactgc
      661 gggaaaaata cggcgccagc gttttcatgc aggagccgaa tgtcaaatgc ggctgcggcg
      721 gcctgcggga ataccagaac ctgctttgga tcagcaacgt caaggaacac gccccgacca
      781 ccgccaagct cgtggagaaa aaaatcctgc gcgaaaccga gcgccgccat ctggagcagg
      841 gctacgactt cctgctgcgg gtccgcacgg agatgcatta cctcaacgag cggcccatcg
      901 acatcctgac cctccgcctg cagggcctcg tggcgaccgc cttcggctac ccgcagcgga
      961 acatcctgcg ccgctgcgag gccttcatgc gggattatta tcagcatacg cggcatgttc
     1021 aactgatcgc gcaggccgcc atcgcccggc tggaagtcca gccggaaccc accggcgcca
     1081 gtctgctcca gatgctgcac ctcaaaaaga cgcgggcgga aaaatttgac ggttttgtcg
     1141 caaaagacgg ctatctgtat ccggtctcgc gggatatttt caacgaggac accgcccggc
     1201 tcatgcgcgt cttccagcac gtccagcgcc ggcagctaca gctttccccg gagctgcggg
     1261 atctcatcct gcgccggacg cggctcatgg accgcacctt tcaatatgcc cgggtcaacc
     1321 gcgaggtgtt cctttccatt ctctcgcaca aggggcgggt cgggcccatt ctccgcgaga
     1381 tgcacgaact cggcttcctg ggcaaatacc tgccggagtt cggcgcgctc acctgcctcg
     1441 tgcagcacga attttttcac cgctacaccg ccgatgaaca cacgctcgtg tgcatcgaga
     1501 aactcgacaa cattctcttc agcgaagacg aaaaactgcg gggctaccgc gccctgttcc
     1561 aaaagcagga tgacccggcg atgctctatc tcgccctcct gctgcacgac accggcaagg
     1621 ccgccaatac gcgcaaccac gaggaagcca gcgcggagct cgcccaccgc gtcgcccggc
     1681 ggctgcaatt gactccccag cggcggcgga tgctcatcac gctggtggac gtgcattatt
     1741 cgctctccca gatttcgcag caacgcaatc tcgacgatct gacgaccatc accaccttcg
     1801 ccggcatcgt gaagaatcgg gaaatcctcg acgccctcat gctcatcacg ctggccgatg
     1861 gcatgggcac aagcgacgaa aaatggtccg actggaagga ggccctcgtc tggcagctct
     1921 accgccgcac cgtcgcttac ctcgaacacg gcccggcggc ctttgacctc aagcagaaga
     1981 acctcaccga aattcggtcc gcgctgcgct tgcccaaagc catggaagag gaagtcgaag
     2041 cccacttcca gcacatgccg gggcgctatt tccgctcgtt taatgccgat caggttcgcc
     2101 agcacctccg cctgtttcac accttcttcg aacgccgcgc cggcggcgag gatccgatca
     2161 aaccggccgt cgaatggatc cagcatcccg ggcagggcca taccgaggtt tgcatctgcg
     2221 gctgggaccg gcacgcgctg ctcgaaaaaa tcgcgggcgc ctttctctcc gccgagatca
     2281 acatcctcag cgcggatgtg ttcacgcgcg gcgacagcct cgtgctcgat ctcttccgcg
     2341 tgtgcgacac ccggcaccag cccgtcacca gccccaagga tttcgcccgg gtcgaatccc
     2401 gcctcaacga ggcgctgcgg cacgaggatt acgacttcac gcccctgctc gcgcgcgagg
     2461 cgcgaatgaa attctaccgc ctctcgcagg acgcggagct gcccacgcgc atcgtgatcg
     2521 aaaacgacgc gcacccgatt tattccatca tcgaaatcca gacccccgac cgcctcggcc
     2581 tgctttacga cttgctccgc gcgttcggcg cggcggatgt ccacatcgaa ctctcccgca
     2641 tcaccacgga aatggacgtc gccatggaca gcttctacgt caccgaattg gatggccgga
     2701 agctcaccga caaagccgcc atcgcccgcc tgcaggagct gctgctgaaa gcttcggcgc
     2761 ggaagcgggc ctgaccgggc gatcctcgag tatttctcaa aagggatttc cgaaaaagga
     2821 ttcaatgcag gggcgcttgg cacaaccgct ctccaccgcg tttggtattt ttgggattaa
     2881 ccacagagaa cacggagggc acagagaggg gaaaatgggg atttcgggat tctttttcgg
     2941 cgtttgaggt gcgcggggcc agcgcgtccc gccgtgaacc cgtttcccgg tggggcggcg
     3001 ttgccaacgc cgccgctccc agcaaccgtt ttaagctcgc gtaaaatgaa gcaacggacg
     3061 gctcggcgtg ccgtccctgc cttttcatgg gcagggcggg ttcgccgagc ccgccgtggc
     3121 ttgggggcgg cgacgctcgt ggcgcgcggg gccagcgcgc cctaccccga atcagcctct
     3181 cgatgggacg ccgttgccaa cgccgccgct cccggcggca aagggacaac caccgaggac
     3241 accgagagta aaacgcggaa tgaaaaaatc ccgaggcttc ttccctctgt gtcctttgtg
     3301 ttctccgtgg tttattcgct gttttggggt tagcgcattt tcacaaatgc tctaaaagcg
     3361 gctgaacacc cggagaatcc actccggata caccccgagc accagcgtgg ccgcgatcaa
     3421 ccccaccagc gccagccggg tcagccgcgt gaccggaatc ggcgccgacc gctcgggcga
     3481 atcccccacc ggcatccaat acatggcgcg cacgattttc agataataat aaaatccgca
     3541 cgccaccgag
//
LOCUS       DATDAY010000184         2476 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_909613, whole genome shotgun sequence.
ACCESSION   DATDAY010000184 DATDAY010000000
VERSION     DATDAY010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2476)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2476)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2476
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_909613"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1322
                     /locus_tag="VIS74_03610"
     CDS             <1..1322
                     /locus_tag="VIS74_03610"
                     /inference="COORDINATES: protein motif:HMM:NF014156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 36 protein"
                     /protein_id="HEY5792358.1"
                     /translation="HWMKTLAPGESLVSPPATVSCVRGDLDDLCDRLTAVQHRAADRQ
                     PEIERDLPIVFNEWCTTWGDPRHEKVAAIAQRLRGSGVRYLVIDAGWYKQGTADWFYG
                     HGDWIPNRALFPHGLEATAAAIREAGLVPGLWFEMETCGRDSAAFSRADHLLQRDGQP
                     LTVGGRRFWNLNDPAAVAYLTERVIGLLERGNFGYLKVDYNETIGLGSDDPDGLGEGL
                     RKQIEGVYRFFDLIRRRLPHLVIENCASGGHRLEPSMLGRTAMSSFSDAHELPEIPII
                     AASLHRLLLPRQSQIWAVLHRADSDQRLAYSLAATFLGRMCLSGEIAGLSDSQMELVA
                     RAERLYRRAAPVIKHGSSRRFGRMGPSWRHPQGWQAVVRTAPDGKSALVVVHAFEGSP
                     EEVRIPLPPGGWEIEEEFPAEVSQVEPDGLTCPALADFSARVIRLRA"
     gene            1335..>2476
                     /locus_tag="VIS74_03615"
     CDS             1335..>2476
                     /locus_tag="VIS74_03615"
                     /inference="COORDINATES: protein motif:HMM:NF017280.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HEY5792359.1"
                     /translation="MSDSPSSMTPPATWEIPQLLRPPAAFPAGEISADGLRPLYYEGP
                     PFRGRPTRVFAFYGAPKAAGKAPGIVLVHGGLGTAYADWVRLWNSRGYAAIAFDHGGH
                     LPVKENGEWKINPDGGPLMDDGKQVRDPVPDQWMHHAVADTVLAHSLLRSFPEVDAER
                     TGITGISWGGVVTSIAVTLDPRFRFAAPVYGCGFISTLNDTGDCFVGRDFPPGDRALW
                     NALWDPEHFLARVRLPMLWVNGTNDFAFTPGAWQRSYRTAPGPRHLSLKVRMPHGHGG
                     PGESPEEIRAFADSVVGGGAGLPRIVEQGRDWLRYESPGPIHHAGLNYTEGRGPWQER
                     EWKTIPAELLASSRTVRAKVPAGASAWYFNLTDEQGLVISSEHEASP"
BASE COUNT          377 a          844 c          837 g          418 t
ORIGIN      
        1 gccattggat gaaaacgctc gcgccgggcg agtcgctcgt ttccccgccc gccacggttt
       61 cctgcgttcg cggcgatctc gacgacctct gcgaccgcct caccgccgtc cagcaccgcg
      121 cggcggaccg gcagccggag atcgagcggg atctgccgat cgtcttcaat gaatggtgca
      181 cgacctgggg cgatccgcgg cacgagaaag tggccgccat cgcgcagcgc ctgcggggct
      241 ccggggtccg ttacctcgtc atcgacgcgg gctggtataa gcagggaacg gcggactggt
      301 tttacgggca cggcgactgg attcccaacc gcgccctgtt cccgcacggc ctggaagcaa
      361 ccgccgcggc aatccgggaa gcgggcctgg ttcccggcct gtggtttgag atggaaacct
      421 gcgggcgcga ttcggcggct ttctcgcgcg cggaccatct gctccagcgc gacggccagc
      481 cgctgaccgt gggcgggcgg cgtttctgga acctgaacga cccggcggcc gtcgcttatc
      541 tgaccgagcg ggtgatcggc ttgctggagc gcggcaactt cggctacctc aaggtcgatt
      601 acaacgaaac catcggcctc ggcagcgacg atcccgacgg cctcggtgag ggcctgcgca
      661 aacagatcga aggcgtttac cgcttttttg acctcattcg ccggcgcctg ccgcatctcg
      721 tgatcgaaaa ttgcgcgtcc ggcggccacc gcctggagcc ttcgatgctc gggcgcaccg
      781 ccatgtcctc gttttccgac gcgcatgaac tgccggaaat tccgatcatc gccgccagcc
      841 tgcaccgcct gttgctgccg cgccagtcgc agatctgggc cgtgctgcac cgcgccgaca
      901 gcgaccagcg cctggcttac agcctggccg ccacgtttct ggggcgcatg tgcctctcgg
      961 gggagatcgc cgggctgtcc gattcgcaga tggaactcgt cgcgcgggcg gagcgccttt
     1021 accgccgcgc cgccccggtc atcaagcacg gttccagccg ccgtttcggc cggatgggtc
     1081 cgagctggcg ccatccgcag ggctggcagg cggtcgtccg gacggccccg gacggaaaat
     1141 cggccctggt ggttgtccat gctttcgaag gcagcccgga agaggtccgg attcccctgc
     1201 cacccggcgg gtgggaaatc gaggaggaat ttccggcgga ggtttcccag gtggaaccgg
     1261 acgggctgac ctgccccgcg cttgccgact tcagcgcccg cgtcatccgg ctgcgcgcgt
     1321 gaccgttttc gcttatgagt gattccccat cctccatgac cccgcccgcg acctgggaaa
     1381 tcccgcaact cctgcggccc cccgccgctt tccctgccgg ggaaatcagc gccgacggct
     1441 tgcggccgct ttattacgaa ggtccgccgt tccggggaag gccgacgcgc gtcttcgcgt
     1501 tttacggcgc gccgaaggca gccggaaagg cgccgggcat cgtgctcgtc cacggcggcc
     1561 tcggcacggc ctatgccgat tgggtgcggc tgtggaattc gcgcggctac gcggccatcg
     1621 cgttcgacca cggcgggcat ctcccggtga aggaaaacgg cgaatggaaa atcaatccgg
     1681 acggcgggcc gctcatggac gatggcaagc aggttcgcga cccggtgccg gaccaatgga
     1741 tgcatcacgc cgtggcggac acggtgctgg cgcactcgct gctgcgctcc tttccggaag
     1801 tggatgcgga gcggacgggc attacgggca tttcctgggg cggggtcgtc acctcgatcg
     1861 ccgtgacgct ggacccgcgc ttccggttcg ccgcgccggt ctatggctgc gggttcatca
     1921 gcacgctgaa cgacaccggg gattgtttcg tcggcaggga tttcccaccc ggggaccgcg
     1981 ccctctggaa cgcactctgg gatccagagc actttctggc ccgcgtccgc ctgccgatgc
     2041 tctgggtcaa tggcaccaac gacttcgcgt tcacgcccgg agcctggcag cgctcgtatc
     2101 ggaccgcgcc gggaccgcgc catctttcgc tgaaggtgcg gatgccccac gggcacggcg
     2161 gtcccggcga atcgcccgag gaaatccggg ccttcgcgga cagcgtggtt ggcggcggcg
     2221 ccgggctgcc gcgcatcgtg gaacagggcc gcgactggct gcgttacgag tcgcccgggc
     2281 cgatccatca cgcggggttg aattacaccg aaggccgcgg cccgtggcag gagcgggaat
     2341 ggaagacgat cccggcggaa ctcctggcgt cgagccgcac ggtccgcgca aaagtgccgg
     2401 ccggcgcctc ggcctggtat tttaacctca ccgacgaaca agggctcgtg atcagcagcg
     2461 agcatgaagc gtcccc
//
LOCUS       DATDAY010000185         2413 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_1031732, whole genome shotgun sequence.
ACCESSION   DATDAY010000185 DATDAY010000000
VERSION     DATDAY010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2413)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2413)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2413
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1031732"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..760)
                     /locus_tag="VIS74_03620"
     CDS             complement(<1..760)
                     /locus_tag="VIS74_03620"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY5792360.1"
                     /translation="MDPVRNPFAPGAGTPPPELAGRDELRETIRIALERTRLGRPAKS
                     ILMVGLRGVGKTVLLDRMREDAEAVGIHTLRVEAPESRSLPAILAPQLRQALLRLSRN
                     EQAKDLARRALRGLAGFAKALKMKYADIEVGFDFEPEPGLADNGDLEHDLQALLEAAG
                     AAAQKAGTALVMFVDELQYVEEEELAALITALHRAAQRRLPVVLVGAGLPQLRGRMGR
                     AKSYAERLFDFPEIGPLPPPAARTAIAKPADAEGA"
     gene            complement(960..2411)
                     /locus_tag="VIS74_03625"
     CDS             complement(960..2411)
                     /locus_tag="VIS74_03625"
                     /inference="COORDINATES: protein motif:HMM:NF013704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HEY5792361.1"
                     /translation="MRSARYARLLSAAPGWGAQRERPITEELLHPLQTMWPDIYKVGN
                     TAVERLGYPTQKPEALLERVIKASSNEGDLVADFFCGSGTTAAVAEKLGRKWIATDLG
                     KFGIHTTRKRLIQVQRELKAGGQPFRAFEVLNLGRYERQAYLNISGNLSAKKKEAALA
                     AKEVEFRNLILRAYQAEPFDGRFGETSLPGGDGSPSRPSLDGFFDGKKSGRLVVVGPI
                     NLPVGRLFVEEVITECRKRGASRVDVLAFEFEMGLFPAVLEEAKQKGIDLAPKTIPPE
                     VFDKRAVERGQVRFHDVAYVEATPRCGVVAGIADPGHSGKLTIAIELTDFSVYYSQGV
                     VDSITADLKEGKSEVVCEAGKLVKFSKDKDGIIKRDVLTKLWTDWVDYWAVDFDYESR
                     KEIIKVARNLGVEGAPPGAADAGEFLDFEERWTGAYIFENEWQSFRTRQSRELELLSA
                     PHTYAKPGRYTVAVKVIDIFGNDTMTLVPINAG"
BASE COUNT          438 a          754 c          716 g          505 t
ORIGIN      
        1 ccgcgccctc cgcgtccgcg ggtttggcaa tcgccgttcg cgccgccggt gggggaagtg
       61 gcccgatctc gggaaaatcg aagaggcgtt cagcgtagga tttcgcccgc cccatccgcc
      121 ccctcaactg cggcagaccc gcgccgacta acacgactgg caaacggcgc tgggccgcgc
      181 gatgcaatgc cgtgatgagc gcggcgagtt cctcttcttc gacgtattgc agttcatcga
      241 cgaacatcac caatgccgtg ccggctttct gcgcggctgc gccagcggct tcgagcaaag
      301 cctgtaaatc gtgctccaga tcgccgttgt cggccaggcc tggttccggt tcaaagtcaa
      361 aacctacctc gatgtcggca tacttcatct tcagcgcctt ggcgaaaccc gccagcccgc
      421 gaagggctcg ccgcgcgagg tccttggctt gttcattccg tgacaaccgc aataacgcct
      481 ggcgtagttg tggcgccaaa atggccggca acgaccggct ctcgggcgct tccacacgca
      541 aagtgtggat tcccaccgct tccgcatctt ccctcatacg gtcaagcaac accgtcttgc
      601 ctacgccccg caggccaacc atcaaaatgc tcttcgccgg acggccaagc cgcgttcgtt
      661 ccaatgcgat acgaatggtc tcgcgcagtt cgtcgcgccc ggccaattcg ggcggtggcg
      721 tgccggcacc gggtgcaaat gggtttctta ccggatccat tcacgtatgt tatgagcagt
      781 ttagcgaatt tacaatcatt ttataaatca cctaagattc atcaaaatag caaaatttcc
      841 gtgccgaaat gcccaacatg ttgcggaggc gcttgtctgg cggggaaacg ggagcccctc
      901 cgccccacat ttggccgcct tttcggaaat cttttttgag agaggctatc aattccgttc
      961 taccccgcat ttatcggcac gagggtcatc gtgtcgttgc caaaaatgtc gatgactttg
     1021 acggcgaccg tatagcggcc cggtttggca taggtgtggg gggcggaaag gagttccagt
     1081 tcgcggcttt ggcgggtgcg gaagctctgc cactcattct cgaagatata ggcgccggtc
     1141 cagcgctcct cgaagtcgag gaactcgccg gcatccgccg cgccgggcgg cgcgccctcc
     1201 acgccgaggt tccgtgcgac cttgatgatc tccttccggc tttcgtaatc gaagtccacc
     1261 gcccagtagt ccacccagtc ggtccatagc ttcgtgagca cgtcgcgctt gatgatgccg
     1321 tccttgtctt tgctgaattt caccagcttg cccgcttcac agaccacctc gctttttcct
     1381 tctttgagat cggcggtgat ggagtccacc acgccttggg agtaataaac ggagaaatcg
     1441 gtaagttcga tggcgatcgt gagtttgccg ctgtggccgg gatcggcgat cccggctaca
     1501 acgccgcagc gcggcgtggc ctcgacgtag gccacgtcgt ggaaacgcac ctggccgcgt
     1561 tccacggcgc gcttgtcgaa gacttccggc gggatggtct tgggcgcgag gtcgatgccc
     1621 ttctgtttgg cctcctccag cacggcgggg aaaaggccca tctcgaactc gaaggccagc
     1681 acgtccacgc gggacgcccc gcgcttgcgg cattcggtga tgacttcctc cacgaacagc
     1741 cgccccacgg gcaggttgat cgggccgacg accacgaggc gtccggattt cttgccatcg
     1801 aaaaatccgt ccagcgacgg acggctcggc gagccgtccc cgccgggtag ggacgtttcg
     1861 ccgaaacgtc catcgaaggg ttcggcctga taggcccgaa ggatcaaatt ccgaaattcg
     1921 acttccttgg ccgccaaggc ggcctccttc tttttggcgg agaggttgcc ggaaatgttg
     1981 agatacgcct ggcgctcgta gcgtccgagg ttgagcacct cgaaggcgcg gaagggctgg
     2041 ccgccggcct tcaactcgcg ctgcacctgg atgagccgct tgcgagtggt gtggatgccg
     2101 aacttgccga ggtcggtggc gatccatttg cgcccgagct tctcggccac ggcggcggtc
     2161 gtgccgctgc cgcagaaaaa gtccgccacc aaatcgcctt cgttgctgct ggctttgata
     2221 actcgctcca acaacgcttc aggcttctgg gttggatagc cgagcctttc aaccgcagtg
     2281 ttgccgacct tgtaaatgtc cggccacatc gtctggaggg ggtgtaaaag ttcttctgta
     2341 attggtcttt ccctctgagc gccccatccg ggggcggcgg agagaaggcg agcgtagcga
     2401 gccgaacgca gac
//
LOCUS       DATDAY010000186         3013 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_180988, whole genome shotgun sequence.
ACCESSION   DATDAY010000186 DATDAY010000000
VERSION     DATDAY010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 3013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3013
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_180988"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(108..935)
                     /gene="cysT"
                     /locus_tag="VIS74_03630"
     CDS             complement(108..935)
                     /gene="cysT"
                     /locus_tag="VIS74_03630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysT"
                     /protein_id="HEY5792362.1"
                     /translation="MAKKRHILPGFGLTLGYTLVYLTLIVLIPVSALVTKTASGGWEH
                     FWTTISSPQVVASYRISFGLSFIAALINGVFGVLAAWVLVRYEFPGRKLLDAIVDLPF
                     ALPTAVAGIALTALYSPKQWLGRYLNELGIAAIFNPLGILIALTFIGLPFVVRTVQPV
                     LQDLSADVEEAAACLGANRWQTFWRVIFPAILPALFTGVTLAYGRAVGEYGSVIFIAG
                     NLPFKTEITPLLIVSKLENYDYTGAAGLGFLMLLASLVILLISNGLQHWQAKRQGVA"
     gene            complement(1056..1859)
                     /locus_tag="VIS74_03635"
     CDS             complement(1056..1859)
                     /locus_tag="VIS74_03635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792363.1"
                     /translation="MNTYEITYQQLKERFEKSGQDVQGFLAPLIALSDDSSSLIVGYG
                     SDQTSIREDLIPYFHLVGRRIEGQEPLRALAVGSWVGTEPASSLAVARLVAALETRLS
                     LAHDMEVTAYPVLNIEAYREGAFLTGKQQLEGVHVWQDSKCSHVVVIERELQRYAYDL
                     ILNFRVNPHSADLTVEGWAESEVRGQIFSDPLARFAEVSPAFHWELNPKKGSFSRIFT
                     PLPGRFEQPVEVAVGLPGMLSADEQAAAGLRIALSLLHQFRQARMEGML"
     gene            complement(1972..2520)
                     /locus_tag="VIS74_03640"
     CDS             complement(1972..2520)
                     /locus_tag="VIS74_03640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEY5792364.1"
                     /translation="MKLSKRGEYALRAMIDLGIAEQAGRELVRLQEIAGKENIPLTFL
                     EQILMQLRMAGFVAAKRGKAGGYYLTRPTEEITIGQIVRLVDGPLAPLGCVSQTAYEK
                     CSCPDEEHCGLRMLMLDVRNAIAGILDRYTLAQVVEVTLRKLRRNEMPIPFAEWLGGE
                     CAAGASANTPNPPRQKKAGTVR"
     gene            complement(2573..>3013)
                     /locus_tag="VIS74_03645"
     CDS             complement(2573..>3013)
                     /locus_tag="VIS74_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HEY5792365.1"
                     /translation="KVAPYPFTTLHPYVGVVELPEYQRVTVADIPGLIEGAHENKGLG
                     HEFLRHILRCRRLLFVLDMAGSEGRDPLDDLASLRKELDLYDVTLSGKAWSLIANKMD
                     LPGAEENLAILRQRFGRVPILPISGKEKQGIDAVKQHLAAVAAG"
BASE COUNT          608 a          962 c          915 g          528 t
ORIGIN      
        1 aaccaggacg atggcaatca agagcgcccg cacccagaac ggctcggagg aagccttgcg
       61 cggggccacc cggttcgcgc ggaccgccaa ctgagtcgca ccggccatta tgccaccccc
      121 tggcgtttgg cctgccagtg ctggaggccg ttgctgatga gcaggatgac gagcgaggcc
      181 aggagcatga ggaagcccag ccccgcggcg ccggtgtagt cgtaattctc cagcttgctc
      241 acaatcagga gcggggtgat ctcggttttg aagggcagat tgcccgcgat gaaaatgacc
      301 gagccgtatt cgcccaccgc ccgcccgtag gccagggtca cgccggtaaa gagcgcgggc
      361 aggatcgccg gaaaaatcac ccgccagaag gtctgccagc gattcgcgcc caggcaggcc
      421 gcggcctcct cgacgtccgc cgacagatcc tgcaggaccg gctgcacggt gcgcacgacg
      481 aacggcagcc cgatgaacgt cagcgcgatc aaaatgccga gcgggttaaa aatggcggcg
      541 atgccaagct cgttgagata acgccccagc cattgcttgg gcgaatacag cgcggtcagc
      601 gcgatgcccg ccaccgccgt gggcagcgca aaaggcaggt ccacgatggc atcgagcagc
      661 ttgcgtccgg ggaattcata ccgcaccagc acccaggcgg cgaggacgcc gaagacgccg
      721 ttgatgagcg ccgcgatgaa ggacaggccg aagctgatcc ggtaggatgc caccacctgc
      781 ggagaggaaa tagtcgtcca gaaatgctcc catcccccgc tcgcggtttt ggtgacgagc
      841 gcggagacgg ggatgagcac gatcagcgtc aggtacacca gcgtgtaacc cagcgtgagc
      901 ccgaatccgg gcaggatatg acgtttttta gccaaaataa aaaagtgaag acaaggtgag
      961 gcgcattctc ttccttaatg cgccagctga gggaatcggc ggcccgcacg ccgccggtga
     1021 gcggtaaact aggaacgaat caacacgaca tgggcttaca gcatcccctc catgcgagcc
     1081 tggcggaatt gatggagtag gctgagggcg atcctgagcc cggcggccgc ctgttcgtcg
     1141 gcggaaagca tgcccggcag gccgacggcc acttcgaccg gttgttcaaa ccggcccgga
     1201 agcggcgtga aaatccggct gaagctgcct ttcttcgggt tgagctccca atgaaacgcc
     1261 ggcgacactt ccgcaaaacg agccagcggg tcgctgaaaa tctggccgcg cacttcactc
     1321 tccgcccagc cttccacggt caggtccgcc gagtggggat tcacgcggaa attcaaaatc
     1381 agatcatacg catagcgctg caattcccgc tcgatcacca cgacatgcga gcatttggaa
     1441 tcctgccaga catgcacgcc ttcgagttgc tgcttgccgg tcaaaaaagc gccttcgcgg
     1501 taagcttcga tattgaggac cggataagcg gtcacctcca tgtcatgagc cagggagagc
     1561 cgcgtctcca gggccgccac cagccgggcc accgccaggc tgctggccgg ctccgtgccg
     1621 acccagctgc cgacggccag ggcgcgaagc ggctcctggc cttcgatccg ccgccccacc
     1681 aggtgaaaat acgggatgag atcctcccga atgctcgtct ggtcgctgcc gtaaccgaca
     1741 atcaacgaac tcgaatcgtc actcaacgca atcaacggcg ccagaaatcc ctgcacatcc
     1801 tgcccggatt tttcaaagcg ctccttcagt tgctgatagg taatttcgta cgtgttcatt
     1861 cccccgcctc ctaatgttga ctgttttggt aaggaatcgt ttttggagaa aaaatcaagt
     1921 taaaatgggc ggcaaaaaag acgcattgat cgcgacgaat ccgcaggcgg attatcgtac
     1981 ggttcctgcc tttttctgac ggggcggatt cggggtattc gccgatgcgc ccgccgcgca
     2041 ttccccgccc agccattccg caaacgggat cggcatttca ttccgccgca gcttgcgcag
     2101 ggtcacctcg accacctgcg cgagcgtgta acggtccagg atgccggcga tcgcgttgcg
     2161 cacgtcgagc atcagcatgc gcagcccgca gtgttcctcg tccgggcacg aacatttctc
     2221 gtaggccgtc tggctgacgc agcccagcgg cgcgagcggg ccgtccacga gccggacgat
     2281 ctggccgatg gtgatctcct ccgtgggccg ggtgagataa taaccgccgg ccttcccgcg
     2341 cttcgccgcg acgaatccgg ccatgcgaag ctgcatgagg atttgctcca ggaacgtcag
     2401 cgggatgttt tcctttcccg cgatttcctg aagccgcacg agttcgcgcc ccgcctgctc
     2461 ggcaatgccg agatcgatca tggcccggag cgcatattcg ccgcgcttgg ataacttcat
     2521 acgtcaaccc cccttgtagg cccgcgggcc gggtccgcgc aagcggcgtt ttttatccgg
     2581 ccgccacggc ggccagatgc tgcttcaccg cgtcgatccc ctgcttttcc ttgccggaaa
     2641 tgggcaggat cggtacgcgg ccgaatcgct ggcgcaggat cgccagattt tcctccgcgc
     2701 ccggcaggtc cattttattc gcgatgaggc tccacgcctt gccggagagc gtcacgtcgt
     2761 aaagatcgag ctccttgcgc aggctcgcca ggtcgtcgag cggatcgcgg ccctcgctgc
     2821 ccgccatgtc gaggacaaac agcagccggc ggcagcgcag gatgtgccgc aggaattcgt
     2881 ggccgagccc cttgttttca tgcgcgccct cgatgagccc cggaatgtcc gccacggtca
     2941 cgcgctggta ttccggcaat tccaccacgc cgacgtaggg atgcagcgtg gtgaagggat
     3001 aaggcgccac ctt
//
LOCUS       DATDAY010000187         2213 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_537122, whole genome shotgun sequence.
ACCESSION   DATDAY010000187 DATDAY010000000
VERSION     DATDAY010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2213)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2213)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2213
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_537122"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..565)
                     /locus_tag="VIS74_03650"
     CDS             complement(<1..565)
                     /locus_tag="VIS74_03650"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HEY5792366.1"
                     /translation="MQLQIFLDSRKFGPGVVDGQPGEFTAKALALYRHSKGLPEEARP
                     DLEGISPYTTYEVTAADLAAIGTMAEEPAEIAKQKRLPYSGLGELLGERFHTTRAFIA
                     ELNPKVTVDALAAGAVVKVPNVARPFHADKFPAAYSKAGASASRSVLVDTRLRLLEVR
                     ESGRLIAAFPITPGSTEHPAPVGEWKVA"
     gene            789..1847
                     /locus_tag="VIS74_03655"
     CDS             789..1847
                     /locus_tag="VIS74_03655"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase family protein"
                     /protein_id="HEY5792367.1"
                     /translation="MIASRHSLDPWLARPEPLLALAPMQDVTDLPFWRLMAQFGGADV
                     YYTEYFRVTADSRLDKPILASITQNPTGRPVIAQMIGNHIPALVRTARELEQHPVAAI
                     DLNLGCPAPVVYRKCAGGGLLRDLPLVDAILGALREAISGKFTVKTRLGFDDERTLDA
                     LLPIFQKHSLDLLTVHGRTVKQMYRDGVRYDRIARAVEAMSCPVLANGNVSSPAAAGA
                     ILAETGARGLMIGRGAVRNPWIFDQIRRRLAGKPPALPAGRDVLAYLHALYEATSPPQ
                     AVEIQQVQKMKKYANFIGAGVEPTGEFLHAIRRAGTRADFFAVCARHLDHGDPMPLEV
                     FPLPARAEGVSEFAPVPN"
     gene            1926..>2213
                     /locus_tag="VIS74_03660"
     CDS             1926..>2213
                     /locus_tag="VIS74_03660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792368.1"
                     /translation="MIAIIVLAGVLPLILSLAALEITGYRHLISTRGAVYEREATHVA
                     LTLHRILERDIRNLTSLITVGQVPELIRQIESTSPPPAGADVPALDARWPAL"
BASE COUNT          383 a          777 c          690 g          363 t
ORIGIN      
        1 cggcgacctt ccattcgcca accggcgcgg gatgttcggt ggagccgggc gtgatgggaa
       61 aggcggcgat caggcggccg ctctcgcgga cctccagcag gcggaggcgc gtgtccacca
      121 ggacgctgcg cgacgccgaa gccccggcct tggagtaagc ggcggggaac ttatccgcgt
      181 ggaaggggcg cgccacgttc ggaaccttca ccaccgcgcc cgcagccagc gcatccacgg
      241 tgactttcgg attcagttcg gcaatgaagg cccgcgtcgt gtggaagcgt tcccccagca
      301 attccccgag gccggagtag ggcaggcgct tttgcttcgc gatctccgcc ggctcctccg
      361 ccatcgtccc gatggccgcg agatccgcgg cggtcacctc ataagtcgtg taaggcgaaa
      421 ttccctccag atcgggccgg gcctcttcgg gcagcccctt ggagtgccgg tatagcgcga
      481 gggccttcgc ggtgaactcg ccgggctggc cgtccaccac gccggggccg aattttcggg
      541 aatcgagaaa gatctgcagc tgcacggccc gatcccgctt tgaaagacgg ggagccgacg
      601 gtcccgtcgt cgcgcagccg cccagtccca aaagcacaat ccccaaaacc agaaaatgac
      661 gaatcatgcc ggaacactga aggggctccc accggaccgc aagagccaaa acccgccatc
      721 cgccttgcgc cagccgccgg catttcctac cttgcgggaa tggtccccaa gcggctcaac
      781 catcccgaat gatcgcgtcc cgccattccc tcgatccctg gctggcccgg cccgagccgt
      841 tgctcgcgct cgcgcccatg caggacgtga ccgatctgcc gttctggcgg ctgatggcgc
      901 aattcggggg cgcggacgtg tattacacgg aatacttccg cgtgaccgcc gactcgcggc
      961 tggacaagcc catcctggcc tccatcacgc agaatcccac cgggcggccg gtcatcgccc
     1021 agatgatcgg caatcacatt cccgcgctgg tccgcaccgc gcgggaactg gaacagcatc
     1081 cggtggcggc gatcgacctg aacctcggct gccccgcgcc ggtggtctat cgcaaatgcg
     1141 cgggcggcgg cctgctgcgc gatctgccgc tggtggacgc cattctgggc gcgctgcgcg
     1201 aagccatttc cggaaagttc acggtgaaga cgcgcctcgg cttcgacgac gaacgcacgc
     1261 tggatgcgct cctgccgatc ttccaaaaac attcgctcga cctgctcacc gtgcacggcc
     1321 ggacggtgaa gcagatgtat cgcgacggcg tgcgttacga ccggatcgcc cgcgcggtcg
     1381 aggcgatgag ctgcccggtg ctggccaatg gcaacgtttc ctcgccggcg gcggccggag
     1441 ccatcctagc ggagaccggg gcgcggggcc tcatgattgg ccgcggagcg gtgcgcaatc
     1501 cgtggatctt cgatcagatc cgccgccggc tcgccggaaa accgccggcc ctgcccgccg
     1561 gccgggacgt gctggcctac ctccacgcgc tctacgaggc gaccagcccg cctcaggccg
     1621 ttgaaatcca gcaggttcag aaaatgaaaa aatacgcgaa tttcatcggc gcgggcgtcg
     1681 agcccaccgg ggaattcctg cacgccatcc ggcgggccgg cacccgcgcg gacttctttg
     1741 ccgtctgcgc ccggcatctg gaccacggcg acccgatgcc gctggaggtt tttccgcttc
     1801 cagcccgcgc ggaaggggtt tcggaattcg ctccggttcc taattgattt tttcggcaac
     1861 tcatccgatg ctggcttcaa ccccctgaag taacgcacat gatgagatgg agcctgcgaa
     1921 ccaaaatgat cgccatcatt gtgctggcgg gagtcctgcc cttgatcctc agcctggcgg
     1981 ctttggaaat caccggatat cgccacctca tctccacgcg cggagccgtt tacgaacggg
     2041 aggcgaccca tgtggccctg acgctccacc gcatcctgga gcgcgacatc cggaacctga
     2101 ccagcctcat caccgtgggg caggtccccg aattaatccg gcagatcgaa tccacctccc
     2161 ccccgcccgc gggcgcggac gtcccggcgc tggacgcgcg ctggcccgcc ctg
//
LOCUS       DATDAY010000188         5664 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_1713258, whole genome shotgun sequence.
ACCESSION   DATDAY010000188 DATDAY010000000
VERSION     DATDAY010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 5664)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5664)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5664
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1713258"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..290
                     /locus_tag="VIS74_03665"
     CDS             <1..290
                     /locus_tag="VIS74_03665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792369.1"
                     /translation="QIRLEQTIIPEISLKDATLTSVLGYLSKKAGDAQPLNFVPQYPR
                     EYGDKTVTLQLSQVPLKVVLDYIGDLAGVRFIPDPYAIRVVAKTPATDSAL"
     gene            complement(226..1011)
                     /gene="truA"
                     /locus_tag="VIS74_03670"
     CDS             complement(226..1011)
                     /gene="truA"
                     /locus_tag="VIS74_03670"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEY5792370.1"
                     /translation="MKLKLVLAYDGQPYDGWQSQAGGNAVQDHLEAAVARICGGRVAV
                     HGSGRTDAGVHAEGQVAHFSPPETSRMQPADWQRALNAGLPPRIRVMGCGFVPEAFHA
                     RFSALGKRYRYVICNRPVLPPAEVGRAWHLPMPLDFDRLQAAAQRCLGTHDFRSFAAN
                     RGRPEKDTVRRITLARWKAGRDLTFEVEGDGFLYKMVRLLVGASVRVAQGKAEIGWIE
                     DLLAHPALGRNSHVAPAGGLCLVRVRYPSPASLRPPGSRKDRE"
     gene            complement(1011..1430)
                     /gene="ruvX"
                     /locus_tag="VIS74_03675"
     CDS             complement(1011..1430)
                     /gene="ruvX"
                     /locus_tag="VIS74_03675"
                     /inference="COORDINATES: protein motif:HMM:NF015607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEY5792371.1"
                     /translation="MNEPILGIDTGDRRCGLALSDDLRMLAHPLETVDVRQTDVFARI
                     AEVVKTRRVALVVIGHPRNMDGSYGPMAEKAKLFAEKLRGQLPCPVKLWDERLTTVAA
                     SRALQEAGRNTRKQKGVIDQAAAQIILQSWLDAHPEL"
     gene            1489..1791
                     /locus_tag="VIS74_03680"
     CDS             1489..1791
                     /locus_tag="VIS74_03680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792372.1"
                     /translation="MSSLSIEKFAENLPVSGVVGLSQAAVGFGAGLLIAGKLEKDSRD
                     KVAISLLAAGAAILIPVVAGVFTRVSNRPESARRIRRQLESIRQDAGLPEHEEEIV"
     gene            1806..2351
                     /locus_tag="VIS74_03685"
     CDS             1806..2351
                     /locus_tag="VIS74_03685"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HEY5792373.1"
                     /translation="MASASPRRRELLEARGWTLRVEPSDITELEDGALPPRELALSNA
                     KAKALDVAGRFPGDCVIGADTIVCLDGRVYGKPRDLEHARAMLTALSGRVHEVMTGVC
                     LVRNRQLCGFVDVSWVKFHPLTDEMIFAYFQRVNPLDKAGAYAIQEDQGALVAKYEGA
                     LENIIGLPILRVEAALRQHFL"
     gene            2394..2864
                     /locus_tag="VIS74_03690"
     CDS             2394..2864
                     /locus_tag="VIS74_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HEY5792374.1"
                     /translation="MSKEVVQVSVHAVVPTNSGCAVFIGDDEKVFVIYVDPGVGSAIS
                     MFINGTTKERPLTHDLIGLILTSLGAKVERIVINDLQSGTYFGRLILSAENEIQQRKI
                     VELDARPSDCIALACQQRAPIYVSKPVWEEVEDMSDVLRKLGDQKESGEEEEEG"
     gene            2916..3308
                     /locus_tag="VIS74_03695"
     CDS             2916..3308
                     /locus_tag="VIS74_03695"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY5792375.1"
                     /translation="MPYSSGMRPLSNYEQKLDPLRDHCPVRAAIDVIRGRWKPSILRE
                     LHEGIKRFSELQAALPGITAQVLTVQLRQLEADGVVARTVHPEEMPVRVEYALSQNGS
                     ALSEVMDQLDKWGSAYLERQAKRGSLVS"
     gene            3396..4100
                     /locus_tag="VIS74_03700"
     CDS             3396..4100
                     /locus_tag="VIS74_03700"
                     /inference="COORDINATES: protein motif:HMM:NF014573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEY5792376.1"
                     /translation="MKSLLLINASGRVTRSITRQLTARFAEGWKSRHPRAEIVIRDVG
                     IHPPPAINETWIAAAFAEPGNHTPAMRKAILLSDTMVDELHRADVIVLGVPMYNYGMP
                     GQLKAYFDQIVRIGRTFAFDPTAENPYLPLIKTKPVVAVLSAGNGAIHPGGELAHLNF
                     LEPHLLTLLGFIGLSDPTVVRVGYDEYQDDRFHQSLAAAETALDQILDKLALNAAPAG
                     FFHHSNPPSHFTPLSL"
     gene            4097..4573
                     /locus_tag="VIS74_03705"
     CDS             4097..4573
                     /locus_tag="VIS74_03705"
                     /inference="COORDINATES: protein motif:HMM:NF020177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthrone oxygenase family protein"
                     /protein_id="HEY5792377.1"
                     /translation="MNHSATLLAFATALGCGLIAGVFFAFSTFVMKALARLPAHEGIA
                     AMQSINVFAVNSGFLAAFLGTSAACLLLAGFSLVRLSEPTAIWLLLGAVIFLAGTFLV
                     TMVCNVPMNNALAALSPTAPGSAERWADYLKNWTLWNHVRTLAALAATGFLIAGLR"
     gene            complement(4664..4804)
                     /locus_tag="VIS74_03710"
     CDS             complement(4664..4804)
                     /locus_tag="VIS74_03710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792378.1"
                     /translation="MVCSQSQRRRKTIYAVCIEADLRAVFFIEGATIWSVDIGAHAIY
                     RA"
     gene            4915..5334
                     /locus_tag="VIS74_03715"
     CDS             4915..5334
                     /locus_tag="VIS74_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HEY5792379.1"
                     /translation="MKTANSILEIRTTGKGTYEITDSVARLVRESGVATGTATVFIQH
                     TSASLIIFENADRSARTDLHEYFERLVPEDSDYFTHTLEGADDSTSHIRMALTRTSET
                     VPIAHGRLQLGTWQGIFVFEHRRSPHRRTVAVNITGV"
     gene            complement(5338..>5664)
                     /locus_tag="VIS74_03720"
     CDS             complement(5338..>5664)
                     /locus_tag="VIS74_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HEY5792380.1"
                     /translation="GVLGFLGVGLGAFGAHKLKPHLQELGTLAAWETAVLYHLIHAVA
                     ILAFAALKLASPLTGWCWLIGTIIFSGSLYVLALTGIKWLGAITPIGGVLFLAGWLLL
                     VFATPK"
BASE COUNT         1076 a         1891 c         1677 g         1020 t
ORIGIN      
        1 cccaaatccg gctcgaacaa acgatcattc cggaaatctc cctcaaggac gccacgctca
       61 cctccgtgct gggctacctt tcgaaaaaag ccggcgacgc gcaacccctg aacttcgtgc
      121 cgcagtaccc gcgggaatac ggcgacaaaa ccgtcacgct tcagctcagc caggtgccgt
      181 tgaaagtcgt gctggattat atcggcgacc tggccggcgt gcggttcatt cccgatcctt
      241 acgcgatccg ggtggtcgca aagacgccgg cgacggatag cgcactctga ccaggcaaag
      301 gccgccggcc ggcgcgacgt gagagttccg ccccagggcg ggatgcgcca gcagatcctc
      361 gatccagccg atctccgcct tgccttgggc gacccggacg gatgccccca ccagcaggcg
      421 caccatcttg taaagaaatc cgtccccctc cacttcgaac gtcaaatccc ggcccgcttt
      481 ccagcgcgca agggtaatgc ggcgaacggt gtccttttcc gggcgcccgc gattggccgc
      541 gaaggaacga aaatcatgcg tgccgaggca tcgctgcgcc gcggcttgca agcggtcgaa
      601 atccaggggc atcgggagat gccacgcccg gcccacctcc gccggcggca gcaccggacg
      661 gttgcagatc acgtagcgat accgtttgcc caaggcggaa aaccgcgcgt ggaatgcctc
      721 cggcacgaat ccgcagccca tcacccggat gcggggaggc aggccggcat tcagcgcccg
      781 ctgccaatca gcgggctgca ttcgcgaggt ctccggcggg gaaaaatgcg ccacctgccc
      841 ctcggcatgc acgcccgcgt ccgtgcggcc ggaaccgtga accgccaccc gcccgccgca
      901 gattcgcgcc acggccgcct ccaggtgatc ctgaaccgcg ttgccgccgg cctggctttg
      961 ccagccgtcg tagggctggc cgtcataggc aaggaccagc ttgagcttca tcacagctcg
     1021 ggatgcgcgt cgagccagct ttgcaggatg atctgcgccg ccgcctggtc gatcacgccc
     1081 ttctgtttgc gcgtgtttcg gcccgcttcc tgcagcgcgc gggaagccgc cacggtcgtc
     1141 aggcgctcgt cccacaattt caccgggcag ggcaattgcc cgcgcagttt ttcggcgaag
     1201 agcttcgcct tttccgccat cggtccgtag gagccgtcca tgttccgcgg atgtccgatg
     1261 accaccagcg ccacccggcg cgtttttacc acctcggcaa tccgggcgaa gacatccgtc
     1321 tggcggacgt ccacggtttc cagcggatgg gccagcatgc gcaggtcgtc gctcagtgcc
     1381 agcccgcagc gccggtcgcc ggtatcaatg cctaaaatgg gttcgttcat gatttaattg
     1441 gtgaaatgcg gttgaaaaaa caccggcaag ttttaggtta tgcgtgatat gtcatccctc
     1501 tccatcgaaa aatttgcgga aaatctcccc gtttccggcg tggtcggcct ctcgcaggcg
     1561 gccgtgggtt tcggggcggg acttctcatc gccggaaaac tggaaaaaga ctcccgtgac
     1621 aaggtggcga tctcgctgct cgccgcgggc gccgcgattc tcattcccgt ggtcgccggc
     1681 gtgtttaccc gggtttccaa ccgcccggaa tccgcccgcc gcattcgccg gcagctggaa
     1741 agcatccgcc aggatgccgg cttgccggag cacgaggaag agatcgtctg aatccaaccc
     1801 tgatcctggc ttccgcctcg ccccgccgcc gcgaattgct cgaggcgcgg ggatggaccc
     1861 tccgcgtgga gccttcggac atcacggaac ttgaggacgg cgccctgccg ccgcgcgagc
     1921 tggcgctttc caacgccaaa gccaaggcgc tggacgtggc cggccggttt ccgggagatt
     1981 gcgtcatcgg cgcggatacg atcgtctgcc tcgacggccg ggtttacgga aaaccgcggg
     2041 atttagagca cgcgcgggcc atgctcaccg ccctctccgg tcgggtgcac gaggtcatga
     2101 ccggcgtctg cctcgttcgc aaccgccagc tctgcggctt cgtggacgtc agctgggtga
     2161 agttccatcc gttgaccgat gagatgattt tcgcctattt ccagcgggtg aatccgctcg
     2221 acaaggccgg cgcctacgcc attcaggaag accagggcgc gctggtggcg aaatacgaag
     2281 gcgcgctgga aaacatcatc ggcctgccga ttttgcgggt cgaagcggcg ctgcgccagc
     2341 attttttgtg agcgcagttg tcgagcgcac cgcaccgtgt tacagctgaa ggagtgagca
     2401 aggaagtggt gcaggtcagc gtgcatgccg tggttcccac gaactccggg tgcgcggtat
     2461 tcatcgggga cgacgaaaag gtgttcgtca tttacgtgga cccgggcgtc ggcagcgcga
     2521 tttccatgtt catcaacggc accaccaagg aacgcccgct cacgcacgat ctgatcggcc
     2581 tcatcctgac ctcgctgggc gcgaaggtgg agcggatcgt catcaatgac ctgcaatccg
     2641 gcacctattt cggacggctg atcctctcgg ccgaaaacga aatccagcag cgcaagatcg
     2701 tcgaactcga cgcgcggccc agcgattgca tcgcgctggc ctgccagcag cgcgcgccta
     2761 tttacgtcag caagccggtc tgggaagagg tcgaggacat gtccgacgtg ctccgcaagc
     2821 tcggcgacca gaaggaatcc ggcgaggaag aagaggaagg ctaagcggcc tcgcttccgg
     2881 tgtcggcctc gcttccggtt gccacggcgg caaacgtgcc ttattcttcc ggcatgcggc
     2941 cgctctccaa ctacgagcag aagcttgatc ccttgaggga tcactgcccc gtgcgggcgg
     3001 cgatcgacgt cattcgcgga cgatggaaac cttcgatcct tcgggaattg catgaaggaa
     3061 tcaaacgctt ttccgagctc caggcggcgc tgccggggat caccgcgcag gtgctcacgg
     3121 tgcaactgcg ccagctcgaa gccgacggcg tggtagcccg gaccgtgcat ccggaagaga
     3181 tgcccgtgcg tgtcgagtat gcgctctccc aaaacggatc cgcgctctcg gaagtgatgg
     3241 atcagctgga caaatggggt tcggcgtatc tcgagcgcca ggccaaacgc ggatcgctcg
     3301 tgagttgacg aggcgggcgc cggtcacttt ccggtgaccc gctcacaatc ttgggacgaa
     3361 gcaaggcggg aaaatcgggc gcatcctacg ttcgtatgaa atcactgctc ctcatcaacg
     3421 ccagcggacg cgtcacgcgt tcgatcaccc gtcagctcac cgcacgcttc gccgagggat
     3481 ggaaatcccg ccatccccgc gcggaaattg tcattcgcga cgtcggcatc cacccgccgc
     3541 cggccatcaa cgaaacgtgg atcgccgccg cctttgccga acccgggaac cacacgcccg
     3601 cgatgcggaa ggcgatcttg ttaagcgaca cgatggtgga cgaacttcac cgggcggatg
     3661 tcatcgttct cggcgtgccc atgtataact acggcatgcc cgggcaactg aaggcttatt
     3721 tcgaccagat tgtccgcatc gggcgaactt ttgccttcga tcccaccgcg gagaacccct
     3781 acctcccctt gatcaaaacc aagccggtcg tggcggttct ctccgcgggc aatggcgcca
     3841 tccatcccgg cggcgagctc gcccatctca actttctcga gccgcatttg ctgaccctcc
     3901 tcgggtttat cggactcagc gacccaaccg tcgtgcgggt cggctacgat gaataccagg
     3961 atgaccggtt tcaccaatcc ctcgccgccg cggaaaccgc cctggaccaa atcctggaca
     4021 agctcgccct gaacgcggcg cccgccggat ttttccacca tagcaatccg ccaagtcact
     4081 tcaccccatt atctctatga atcattccgc caccctcctt gcgttcgcca ccgcgctcgg
     4141 ctgcggttta atcgccggcg tcttcttcgc gttttcgacc ttcgtcatga aggcgctggc
     4201 ccgccttccc gcccacgagg gtatcgcggc gatgcaatcc atcaatgtct tcgccgtgaa
     4261 ctccggattc ctcgcggcct ttctcggcac ctccgccgcc tgcctactcc tcgccggctt
     4321 ctccctcgtg cgattgagcg aacccaccgc gatctggctg ctcctcggcg ccgtgatctt
     4381 cctcgcgggc acgttcctgg tgacgatggt ctgcaacgtc ccaatgaaca acgccctcgc
     4441 cgcgctttcg ccgacggctc caggcagcgc cgagcgatgg gccgattacc tgaaaaattg
     4501 gaccctctgg aaccacgtgc ggaccttggc cgcgctcgcc gccaccggct tcctgatcgc
     4561 cggcctccgc tgaaaagcca aactaaaaaa ccggcgccaa tcggtgaaat ctgcggcaaa
     4621 ataacggctg ggaggcagaa accgaaccgc gaaccggacg acgtcaggcg cgataaatgg
     4681 cgtgggcgcc gatatccacg ctccagatcg tcgcgccttc gatgaagaag acggctcgaa
     4741 gatcggcttc gatgcagacg gcgtaaatcg tcttgcggcg gcgttggctt tgagagcata
     4801 ccaaaggtct gcaaaacgga atttttaacc gcggattccg gctgcgggca tctatctccc
     4861 ttccgcttcc acccttgctt gactgagccc acacccttct gagcgaattt gacgatgaaa
     4921 acggcgaact ccatccttga aattcgaacc acgggcaagg gcacttacga aatcaccgat
     4981 tcggtggccc gcctcgtccg ggagagcggg gtggcgaccg gcaccgccac ggtgttcatc
     5041 cagcacacca gcgcgagcct gatcattttt gaaaacgccg accgctcagc gcgcaccgat
     5101 ctgcacgagt atttcgagcg gctcgtgccg gaggattccg actacttcac ccacaccctc
     5161 gaaggcgcgg acgattccac ctcgcacatt cgcatggcgc tgacccggac gagcgagacc
     5221 gttcccatcg cccatggccg cctgcaactg ggcacgtggc agggcatttt cgtcttcgaa
     5281 caccgccgct cgccccatcg gcggaccgtg gcggtcaaca tcaccggcgt ttgagcgtta
     5341 tttcggcgtc gcgaacacca gcagcagcca gccggcgaga aagagcacgc cgccaatggg
     5401 agtgatggct cccagccact tgatgccggt cagggcgagc acgtaaagcg agccggagaa
     5461 aataatcgtg ccgatcagcc agcaccagcc cgtgagcggc gaggcgagct tgagcgcggc
     5521 gaacgcgaga atcgccaccg catgaatgag gtgatacagc acggccgttt cccaggcggc
     5581 cagcgtgccg agctcctgca aatgcggctt gagtttgtgc gcgccgaagg ctcccagccc
     5641 cactcccagg aagccgagaa cccc
//
LOCUS       DATDAY010000189         2656 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_971613, whole genome shotgun sequence.
ACCESSION   DATDAY010000189 DATDAY010000000
VERSION     DATDAY010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2656)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2656)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2656
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_971613"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..859
                     /locus_tag="VIS74_03725"
     CDS             <1..859
                     /locus_tag="VIS74_03725"
                     /inference="COORDINATES: protein motif:HMM:NF013465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HEY5792381.1"
                     /translation="QAEGPLKVASLSTVLSDIAQNVGGGKVEVEWILKPDKDPHAFQP
                     SPSDVKTLSQAKIVLASGKGIEGYLSKLEQSAGPDTRFITVSDSIPSLKMKEAADHDH
                     DHGGAADHGGLVEDPHWWHSVKNAKIATKVIRDALAAADPANADFYKANAAAYQVRLD
                     ALAKWVRGQVALLSADKRKLVTSHDAFQYFARENGFTIYAVEGLSTSDQPTSHEVAEL
                     IQTIKAQGVKAVFAESAVNPKVVQEITQETGAKLGGELLADGLNGKEASTYEDMVRVN
                     VTTIVNALK"
     gene            865..1290
                     /locus_tag="VIS74_03730"
     CDS             865..1290
                     /locus_tag="VIS74_03730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792382.1"
                     /translation="MRGIPLIRLAIVLALFLLAFVPLWQLTAAKPARAAATASAQPPA
                     ANETEPAQIEITPSRPASQFAIRYLGNVIWKGSGGKSAAALDLPRGAFDLQVEASWAP
                     VDGAENALRVRISRDDLPLADASFWAADSIDEVMTVPAN"
     gene            1305..2039
                     /locus_tag="VIS74_03735"
     CDS             1305..2039
                     /locus_tag="VIS74_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEY5792383.1"
                     /translation="MSHQLLIKDLTVSYNRIPAVHHLNAALPCGARVGLLGPNGAGKS
                     TLLKTLAGLIRPETGSIEFQGHAFRGSQRAIAYLPQRESVDWDFPITVRGLAEMGRYP
                     QLGAWGKFGPEDARRVHEALEMMHLADFADRQISALSGGQQQRAFLARAWAQQSQIYL
                     LDEPFTGLDRNSQEHFSAAIRRLSDNHKIVVASHHELRTVPELFDYVILLNGELVAAG
                     PTSEVFTPENIEKTFSIDAFAPHSHA"
     gene            2036..>2656
                     /locus_tag="VIS74_03740"
     CDS             2036..>2656
                     /locus_tag="VIS74_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEY5792384.1"
                     /translation="MISWLTEPFQYEFMQRALLGSVIIGFTNGFLGAFIVLRRLALLA
                     DALSHSLLPGLALAAIFFGLAPAGLFFGALIATIFVGVGGQLIARSSRLKEDTAIGAL
                     FVFAFAIGVILLGYSKVRVDLSHFLFGNVLGISDSDLWISYGIGLVTMAFLAAQHRPL
                     LLALFEPAVAATQGIRVGALNVALMILTVLAMISSLQAVGVVLSLGL"
BASE COUNT          474 a          884 c          791 g          507 t
ORIGIN      
        1 gcaggcggaa ggtccgttga aagtcgcgtc gctcagcacg gtgctcagcg acatcgcgca
       61 gaacgtgggc ggcggcaaag tcgaagtgga atggattctc aagcccgata aggatccgca
      121 cgcgttccag ccgtcgcctt cggacgtcaa aaccctcagc caggcaaaga tcgtgctggc
      181 cagcggcaag ggcatcgaag gttacctttc caaattggag caaagcgcgg ggccggacac
      241 gcgcttcatc acggtcagcg attcgatccc ctccctgaaa atgaaggaag cggcggatca
      301 tgaccacgat cacggcggag ccgccgatca tggcggcttg gtggaggatc cccattggtg
      361 gcacagcgtg aaaaatgcga aaatcgccac gaaggtcatc cgcgacgcgc tggccgcggc
      421 ggatcccgcc aatgcggatt tctacaaagc caacgccgcg gcgtatcagg tccggctgga
      481 tgcgctggcc aaatgggttc gcgggcaggt cgccctgctg tccgccgaca agcgcaagct
      541 cgtgacctcg cacgatgcgt tccaatattt cgcccgcgag aatggcttca ccatttacgc
      601 cgtcgaaggc ttgagcacgt cggatcagcc cacctcgcac gaagtcgccg aattgattca
      661 aaccatcaag gcccaagggg tgaaggccgt tttcgcggaa agcgcggtga atccgaaggt
      721 ggttcaggaa atcacgcagg agaccggcgc caagctcggc ggggaattat tggccgacgg
      781 cctcaacggg aaagaagcct cgacttacga agacatggtt cgcgtcaacg tgacaaccat
      841 cgtaaacgcg cttaaatagg cgggatgcgc ggcattccac tcattcgtct ggcgatcgtc
      901 ctcgcgctgt tcctgctcgc gttcgttccc ttatggcagc tcaccgcggc caaaccggcg
      961 cgggcggccg ccaccgcgtc cgcccagccg ccggcggcca atgaaaccga gcccgcgcag
     1021 atcgaaatca ctccctcccg gccggccagc cagtttgcca ttcgttacct cggcaacgtg
     1081 atctggaagg gaagcggcgg caaatcggcg gcggctctcg atcttccgcg cggcgcgttc
     1141 gacctccagg tcgaggccag ctgggctccg gtcgatggcg cggaaaacgc cttgcgcgtc
     1201 cggatttcgc gggacgatct tcccctggcc gacgccagtt tttgggccgc ggactcgatc
     1261 gatgaagtga tgaccgtgcc ggcaaactag acccaaacca gaccatgtcc caccagcttc
     1321 tcatcaagga tctcacggtc tcctacaacc gcatcccggc ggttcaccac ctgaacgccg
     1381 ccctgccctg cggcgcgcgc gtcggcttgc tcggcccgaa tggcgcgggg aaatccacgc
     1441 tgttgaaaac gctcgccgga ttgatccggc cggaaaccgg cagcatcgag tttcaaggcc
     1501 acgcgtttcg cggcagccag cgggcgatcg cctacctgcc ccagcgcgag agcgtggact
     1561 gggattttcc catcaccgtt cgcggcctgg ccgagatggg ccgttatccc cagctcggag
     1621 cgtgggggaa attcggcccg gaggacgcga gaagggtcca cgaggcgcta gaaatgatgc
     1681 acctcgcgga cttcgcggac cggcagattt ccgcgctctc cggcggccag caacagcggg
     1741 ccttcctcgc gcgcgcctgg gcgcagcaat cccagattta cctgctcgac gaacccttca
     1801 ccgggctgga ccgcaactcg caggaacatt tttccgccgc catccgccgg ctctccgaca
     1861 atcacaagat cgtggtcgcc tcgcaccacg aactgcgcac ggtcccggaa cttttcgatt
     1921 acgtgatttt attgaatggc gagctcgtcg ccgccggccc gacttccgaa gtcttcaccc
     1981 cggagaacat cgagaaaact ttttccatcg acgccttcgc cccgcattcc cacgcatgat
     2041 ttcctggctc accgaaccct tccaatacga gttcatgcag cgggcgctgc tcggctcggt
     2101 gatcattggc ttcaccaatg gcttcctcgg cgccttcatc gtgctgcgcc ggctcgccct
     2161 gctcgcggac gcgctctccc actcgctcct tcccgggctc gcgctggcgg cgatcttctt
     2221 cggcctcgcg ccggcggggc tttttttcgg cgcgctgatc gccaccatct tcgtcggcgt
     2281 gggcgggcaa ttgatcgcgc gcagctcgcg tttgaaggag gacacggcca ttggcgccct
     2341 gtttgtcttt gccttcgcga ttggcgtgat tctgctcggc tactcgaagg tccgcgtgga
     2401 tttgtcgcat ttcctttttg gaaacgtgct cggcatttcc gactccgatc tctggatttc
     2461 ctatggcatc ggactcgtga ccatggcttt cctggccgcg cagcaccgcc cgctgctgct
     2521 cgcgctgttc gaaccggccg tcgccgcaac ccagggcatc cgcgtcggcg cgttgaatgt
     2581 cgcgctcatg attctcaccg tgctggccat gatctcctcg ctccaggccg tcggcgtggt
     2641 gctttcgctc ggcctg
//
LOCUS       DATDAY010000190         2973 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_2133743, whole genome shotgun sequence.
ACCESSION   DATDAY010000190 DATDAY010000000
VERSION     DATDAY010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2973)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2973)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2973
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2133743"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            177..617
                     /locus_tag="VIS74_03745"
     CDS             177..617
                     /locus_tag="VIS74_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY5792385.1"
                     /translation="MSKPTIEPYLTFSGRCEEALEFYKKAIGARVEMVMRFDEAPDPV
                     PEGMLAPGFEKKIMHSSFWVGDSMIMASDGCNEASKFDGFSLSLSLTGEAEAEKIFAA
                     LGEGGKVNMPLGKTFWSPLFGMVEDRFGVSWMVNVLPKDGKGCA"
     gene            699..1814
                     /locus_tag="VIS74_03750"
     CDS             699..1814
                     /locus_tag="VIS74_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HEY5792386.1"
                     /translation="MSKVRVLVGTRKGAFILTADGKRQKWEVSRPHFAGLELYHLKGT
                     PADPNRIFASQYSDWFGQAVHRSDDGGSTWQTVGNAFAYEGATGTHQWYDGTQHPWEF
                     KRVWHFEPSADDPDKVYAGVEDAGLFRSDDGGATWKELPGLRSHETGPLWQPGAGGMC
                     LHTILLDPVNAGRMYVAISAAGVFRSDDAGKTWKPINRGLKSDFLPNPTAEVGHCVHR
                     IAQHPSRPGTLFMQKHWDVMRSDDAGESWREVSGNLPSDFGFPIDVHAHEPETIYVVP
                     IKSDSEHFPPDGKLRVYRSRAGGNEWEALTRGLPQQDCYVNVLRDAMAVDSLDPCGVY
                     FGTTGGQVYASADGGDSWNPIVRDLPAVLSVEVQTLP"
     gene            1811..2107
                     /locus_tag="VIS74_03755"
     CDS             1811..2107
                     /locus_tag="VIS74_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792387.1"
                     /translation="MIRVALPHHLRTLAGAGSEVEVAVDPPVTIAAVLGTLEARYPAL
                     RGAIREHGTLRRRSYVRFFACQEDLSHEPPDYILPDEVTRGEEPFLVIGAIAGG"
     gene            2217..>2973
                     /locus_tag="VIS74_03760"
     CDS             2217..>2973
                     /locus_tag="VIS74_03760"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEY5792388.1"
                     /translation="MLLDTHAHLDYPDYADDLEAVVERAAAAGVTRIISIGTGLASSR
                     RAVALAERFANVFAVVGIHPTNVLDEPEDFLPELREIARHPKVAAIGETGLDYHYLPG
                     EGAKSGEAPLDPAYQNAQAAAFVKQLELANELGLNAVIHQRDAWDDTLRLLDESAGEA
                     RGVFHCFGGSPQQAAEVIDRGHLVSFTGIVTFKNAPLVRETAASIPLGCFMVETDCPY
                     LSPIPHRGKRCEPAYTRLVAEKIAEVRGVPFEKI"
BASE COUNT          554 a          955 c          939 g          525 t
ORIGIN      
        1 agagctcggc aatggcctca cccgctacac ggcgcgcgcc ctccactgga ctgaggagga
       61 tcgcaagaag catgaagaaa tgggcttcga gcccggatgg aaccagtgcc tcgaccaact
      121 cgtcgaactg atgagcggca aataagaatt tccccaacaa cccaaccacc ccacccatgt
      181 ccaaacccac catagaaccc tatctcacct tcagcggccg ctgcgaagag gccctggaat
      241 tttacaaaaa agccattggc gcccgtgtcg aaatggtcat gcgctttgac gaagccccgg
      301 atcccgtgcc cgagggcatg ctcgctcccg gcttcgaaaa gaagatcatg cactcctcct
      361 tctgggtggg tgacagcatg atcatggcct cggatggctg caacgaggct tcgaaattcg
      421 acggattttc gctgtcgctt tccctcaccg gcgaagccga ggcggagaag atttttgcgg
      481 ctctcggcga gggcgggaaa gtgaacatgc ctctgggcaa gaccttttgg tcacccctct
      541 ttggcatggt cgaagatcgc ttcggcgtca gctggatggt caacgttctg cccaaggatg
      601 gcaaaggctg cgcatgaaca ggcagggcgt cccgcggttg cgggaccaac cccgccatcg
      661 acaggcgact catcgagccg cccctgccac tccatctcat gagcaaagtc cgcgttctcg
      721 tcggcactcg caagggtgcg ttcattctca cggctgacgg caaacgtcag aagtgggagg
      781 tcagccgccc gcatttcgcg ggcttggaac tttatcactt gaaaggtacc ccggcggacc
      841 ccaaccgcat cttcgcctcg caatacagcg attggtttgg ccaggcggtg caccgttccg
      901 acgacggcgg ctcgacctgg cagacggtcg gcaacgcatt tgcctatgag ggggcgacgg
      961 gaacccacca gtggtatgac ggcacccagc atccgtggga attcaagcgc gtctggcatt
     1021 tcgagccgtc ggcggacgat cccgacaagg tttacgccgg cgtggaggat gccgggctct
     1081 tccggtccga cgacggcgga gccacctgga aggagctccc cgggctgcgc tcgcacgaaa
     1141 ccggcccgtt gtggcagccg ggcgcgggcg ggatgtgcct ccacacgatc ctgctcgatc
     1201 cggtgaacgc cggccgcatg tatgtcgcga tctcggcggc gggggtcttc aggagcgacg
     1261 atgccgggaa aacctggaag ccgatcaacc gagggttgaa gtccgacttc ctgcccaatc
     1321 ccaccgcgga ggtcggacat tgcgtgcacc gcattgccca gcatccttcg cggccgggca
     1381 cgctcttcat gcagaaacat tgggacgtga tgcggagtga cgacgcggga gagtcctggc
     1441 gcgaagtgag cggcaacctg ccgagcgact tcgggtttcc catcgacgtc catgcccacg
     1501 agccggaaac gatctacgtg gtgccgatca agagcgattc cgagcatttc ccgccggatg
     1561 gcaaactgcg cgtgtatcgc agccgcgcgg gaggaaacga atgggaggcg ctcaccaggg
     1621 gcctgccgca gcaggattgc tacgtcaacg tcctccgcga cgcgatggcg gtggattcgc
     1681 tggatccgtg cggcgtgtat ttcggaacga ccggcggcca ggtatatgcg tctgccgatg
     1741 gcggcgattc ctggaacccc atcgtgcgcg acctgcccgc ggttctttcc gtggaggtcc
     1801 agacactgcc atgatccgcg tcgcgctccc gcatcatttg cgaacgcttg ccggtgccgg
     1861 cagcgaggtc gaggtcgcgg tggatccgcc cgtgaccatc gcggcagtgc ttggcaccct
     1921 cgaggcgcgt tatcccgcgt tgcgcggagc gattcgcgaa cacggaaccc tgcgccgccg
     1981 gtcttacgtg cggttcttcg cctgccagga ggatctctcc cacgagcctc cggattacat
     2041 tcttcccgac gaagtcaccc gcggcgagga acccttcctc gtgatcggag ccatcgcagg
     2101 cggctgagac atgccggatg cgggttgtgg ggaagcggtc ctgcgcgcct ggctcccgcc
     2161 aaaagcgcag aaagaccttg ggagaagcgc cccggattcg gtaagctgaa gggctcatgc
     2221 ttctcgatac ccacgcccat ctcgattacc cggattacgc cgatgacctg gaggccgtcg
     2281 tcgaacgcgc ggcggccgcc ggcgtcacgc ggatcatcag catcggcacc ggcctcgcca
     2341 gcagccgccg ggccgtggcg ctggcggagc ggttcgccaa tgtattcgcc gtggtcggca
     2401 ttcatccgac aaacgtgctg gatgagcccg aggacttttt gcccgagctg cgggagatcg
     2461 cgcggcatcc caaggtcgcc gccatcggcg agaccgggct cgattaccat tatctgccgg
     2521 gagagggagc caaatccgga gaggcgccgc tggatcccgc ctatcaaaac gcgcaggcgg
     2581 cggcgttcgt gaagcaactc gaactcgcca acgaactcgg gctcaatgcc gtcatccacc
     2641 agcgcgacgc gtgggacgat acgctgcgcc tgctcgacga atccgccggg gaggcgcgcg
     2701 gcgtgttcca ttgcttcggc ggttcgccgc aacaggccgc cgaggtcatt gaccgggggc
     2761 atctcgtctc cttcaccggc attgtcacct tcaagaatgc tccgctcgtc cgcgagacgg
     2821 cggccagcat tccgctgggg tgttttatgg ttgaaaccga ctgcccctac ctttcgccga
     2881 tcccgcaccg cggcaagcgc tgcgagccgg cttacacccg gctcgtcgcg gaaaagatcg
     2941 cggaggttcg cggggtgcct ttcgaaaaga ttg
//
LOCUS       DATDAY010000191         2298 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_1768134, whole genome shotgun sequence.
ACCESSION   DATDAY010000191 DATDAY010000000
VERSION     DATDAY010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2298)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2298)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2298
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1768134"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1072
                     /locus_tag="VIS74_03765"
     CDS             <1..1072
                     /locus_tag="VIS74_03765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792389.1"
                     /translation="AQRQALAFLCAQAGAELPIDSARELAAIFHAKPQPESISPLRRL
                     LDHPSLAVARDSAEALEFMGEKAEIPPKLGPVRYRLLVDGKPYAGRKVEWIVKWGNLS
                     TSSEVLTDAGGVAAVPRDMFLDQGRGPVQLVALRSAAMSRPDDPWFSARMPAPPASED
                     VIPVEVKTQPRRVELSLPRPETELRGKTMDVALWGEQGDQGGFWAPARLKLPAARELT
                     FEKLMPGTYRVEIRVPGAASWAGELRAGDPAALKVPLVRASDVKPAVRPPPHWEGLLI
                     PELLKDGKVVSTDWDYENFRLRGVPVGHYVLRVPSSAEIRKRTMALLPDGPEFDAVEV
                     PFEVAPDSPVEIDLGEIKPKVR"
     gene            complement(1146..1574)
                     /locus_tag="VIS74_03770"
     CDS             complement(1146..1574)
                     /locus_tag="VIS74_03770"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HEY5792390.1"
                     /translation="MLLDTNVLIYATLSSDPRHEKAMELLNARSLPGNEFFVSTQNLA
                     EMYPNLTGPKTKPADSAELARRKITSLAGLDRLTVLPVTRAVVERALELCEKHGVIRQ
                     RYFDFQLAALMLLERIPAIATENEADFSGIPEVSAINPFR"
     gene            complement(1576..1785)
                     /locus_tag="VIS74_03775"
     CDS             complement(1576..1785)
                     /locus_tag="VIS74_03775"
                     /inference="COORDINATES: protein motif:HMM:NF013561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HEY5792391.1"
                     /translation="MKSHISATIDSTLVEELEAFSREERRSRSQVIELALDQFLAARQ
                     RKSTRIVTSEAAFSGKFRREDCYDR"
BASE COUNT          464 a          675 c          727 g          432 t
ORIGIN      
        1 cgcgcagcgg caggcgctgg cgttcctctg cgcgcaggcc ggcgcggagc tgccaatcga
       61 ctccgccagg gaattggccg cgatctttca cgcgaagccg caaccggagt cgatctcccc
      121 gctccgccgc ctgctcgacc atccctccct cgccgtggcc cgggattcgg cggaggccct
      181 cgaattcatg ggcgaaaagg cggaaattcc gccgaagctg ggccccgtcc ggtatcgcct
      241 cctcgtggat ggaaagccct acgccggccg caaggtggaa tggatcgtca aatggggaaa
      301 tctcagcacg agctcggaag tcctgaccga cgccggcggc gtggcggccg tgccgcggga
      361 catgttcctc gatcagggcc gcggcccggt tcaactcgtg gccttgcgca gcgcggcgat
      421 gtcccgcccg gacgatccgt ggttcagcgc gcggatgccg gccccgccgg cttccgagga
      481 cgtcatcccg gtcgaggtga aaacccaacc ccggcgcgtc gaactgtccc ttccccgccc
      541 ggaaaccgaa ctgcggggca aaaccatgga cgtggcgctg tggggggagc aaggcgatca
      601 aggtggattt tgggctccgg cccgcttgaa gctgccggca gcgcgggaat tgaccttcga
      661 gaaattgatg ccgggaacct atcgcgtaga aattcgcgtt cccggcgcgg caagttgggc
      721 cggggagctg cgggccggcg atccggccgc cctcaaggtg ccgctggtcc gggcgagcga
      781 cgtcaaaccg gccgtgcggc cgccgcccca ctgggaaggg ttgctcattc ccgagctgct
      841 caaggacggc aaggtggttt cgaccgattg ggattatgaa aatttccgcc tgcgcggggt
      901 gccggtgggg cattatgtcc tgagggtgcc ctccagcgcg gagatccgaa agcggacaat
      961 ggccttgctg ccggacggtc cggaattcga cgcggtcgag gtgccgttcg aggtcgcacc
     1021 ggattcgccg gtggaaatcg acctgggcga gatcaagccc aaggtgcgat aaagagccac
     1081 ggcggaccgg tgatcccgcc ctgccgtaag cccaggccgt caataaccac catcagttcc
     1141 aggggttatc ggaagggatt gatggcgctg acttccggga tgccggaaaa atccgcctcg
     1201 ttttccgtgg caatggcggg aattctttcg agcagcataa gcgccgcaag ttgaaaatcg
     1261 aaataccgct ggcgaatgac gccgtgtttt tcgcagagtt ccagcgcccg ttcgaccacc
     1321 gcacgcgtca ccggcagaac cgtcaggcga tccagaccgg ccagactggt gattttccgg
     1381 cgggcaagtt ccgcggaatc ggcgggtttg gttttgggcc cggtcagatt gggatacatc
     1441 tcggcgagat tctgggtgga aacgaaaaat tcgttccccg gcagcgagcg cgcgttcaag
     1501 agttccatgg ctttttcgtg acgcgggtcg gaactgagcg tcgcgtagat cagaacattt
     1561 gtatccagca gcaccctacc ggtcataaca gtcctcccgg cggaatttgc cggaaaaagc
     1621 cgcctccgag gtgacaatac gggtggattt ccgttgccgg gcggcgagaa attgatcaag
     1681 cgccagttcg atcacctgac tccgtgaccg ccgttcctcc cggctgaaag cttccaactc
     1741 ttccaccaag gtggaatcaa tggtcgcgga gatgtgggat ttcataacac tgcgatgtta
     1801 gctttgatga aaccccacgt caatggatca gatgataaaa gcggctggcg agttgcgcgg
     1861 ttacgatagc gcgctcgccg agacggcgca gccggaatgt gaagcccttg ccgaatctta
     1921 ctttgcggaa cgttaccccg gcttcgatct agacgatccc gattgggatg acttgggcgg
     1981 tcacttggag caaaatatgg gaagaaaagt cacggcgggg tcatcgcccc cgccctgccc
     2041 attgaaggca gggacagctc gatgagctgt ccgtcgcttc gccgcttgcg tccgattgaa
     2101 taaaaagccc aaaccaaaat tcttgcagtt gaccaatcag tcaatacagt ccaagtaagc
     2161 ggatgtggaa atcagggact gatgatgtca aatccttcga aaccggcggg cgtgaggcct
     2221 tccgcggaag aactcgtttg gacgagggcc acccgggatt ttttgattca ataatttact
     2281 aaccagtcaa ttaactta
//
LOCUS       DATDAY010000192         2399 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_220667, whole genome shotgun sequence.
ACCESSION   DATDAY010000192 DATDAY010000000
VERSION     DATDAY010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2399)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2399)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2399
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_220667"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            204..407
                     /locus_tag="VIS74_03780"
     CDS             204..407
                     /locus_tag="VIS74_03780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792392.1"
                     /translation="MSALALGVMTLWAEPASAADKDIATAATIAVGVSESTSAGRSQV
                     VLAGMVAALSALLVLRRHARVCN"
     gene            541..1158
                     /locus_tag="VIS74_03785"
     CDS             541..1158
                     /locus_tag="VIS74_03785"
                     /inference="COORDINATES: protein motif:HMM:NF019284.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system YwqK family antitoxin"
                     /protein_id="HEY5792393.1"
                     /translation="MKSLFASLLIAVVSLQPLFAQDQGAEPGITPTATPTPLTPPLPA
                     LPKASPPSATPAAAVELYSLDYNETLDLFCVPGTPTGYTGRVISTYDNGKQELVGALK
                     AGKREGRWLEYAEEEGTLTSAGDYLDGKETGEWKYWFENGQLLSVGSYTDGKPTGRWK
                     TYYENGKPESEGVYVDGEMDGDWKVFDDETNEVRTLKFKNGEQIP"
     gene            1409..>2399
                     /locus_tag="VIS74_03790"
     CDS             1409..>2399
                     /locus_tag="VIS74_03790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792394.1"
                     /translation="MASEETLPGAADSPSITVSKENAGSEAAPPPAPSEPAEVGVPNR
                     NNFFLPDFDSYGQSFFEREGLKFRAGPVHFRMSLGVGLEYNDNIFGSYLDPVSDTIAH
                     VTPSFQFGIGDFNAKAEEYLLLKYRPSFDYYQSQTQQNRVNQNLEITGQTTFSRYSTA
                     LDLNFQTNSSPNATQSGGQEYSTLNFNWDNRYYLGSKTFARALVGANIQQSENDNNYQ
                     TFSISPQVGYEYSPKTTLFFGPYAGISYVGNGGSQTFQGLTVGINYSNLRKLKFSGTF
                     GVQARQYDGQDTTGASNFITPVFNLGMTYDATETTSLRLMLLRDVQLSDVQRGL"
BASE COUNT          526 a          749 c          664 g          460 t
ORIGIN      
        1 tttttatcca aaaaatttcc ccctcataag cgactggctt tccctgacat gcagcgcccg
       61 ccctcgcggc caaaccatcc atggcctgat tctcctcgcc tctggcatgg gtgctgctat
      121 ttcatttctg cgattctacg acataaccat ctaaaaaccg ttcaaattac ctggggggga
      181 aaatgagaac accaaaactg tggatgagcg cccttgcgct cggagtgatg acattgtggg
      241 cggaacctgc ttccgccgcg gataaggaca tcgccaccgc ggccaccatc gcggtggggg
      301 tgagtgaatc cacgagcgcc ggccgtagcc aggtcgttct ggccggcatg gtcgccgcgc
      361 tttcggcgct cctcgtgctg cgacgacacg ccagggtatg caactgatga cccacggcga
      421 ttccgcgcgg gaattggtgg gttgcgggcc ggaagtgagg cttttgcgtt gacgtgcgaa
      481 cgagccgctc ctattcgatg ggatgcctgc ggctcgtgcg tgcgcgggca atttttccga
      541 atgaaatccc tgttcgccag ccttttgatc gcggtcgttt ccctgcaacc gcttttcgct
      601 caagaccaag gagcggagcc cgggatcacg cccacggcca cccccacgcc gctgacgcca
      661 ccccttccgg ccctgcccaa agcctctccg ccttccgcca ccccggctgc cgcggtggag
      721 ctttactctc tcgactacaa tgaaacgctc gaccttttct gcgttccggg cacgccgacc
      781 ggctacacgg gaagagtgat ctccacctac gacaacggca agcaggagct ggtcggcgcc
      841 ctcaaggccg gaaagcgcga ggggcgctgg ctggaatacg ccgaggagga gggcaccctg
      901 acttcggcgg gcgactacct cgacggcaag gaaaccggcg agtggaaata ctggttcgag
      961 aatgggcagt tgctgagcgt gggcagctac accgacggaa aacccacggg acgttggaag
     1021 acgtattatg aaaacggcaa gccggaatcc gagggcgtct atgtggacgg ggaaatggac
     1081 ggcgactgga aggtcttcga cgacgagacc aatgaagtgc gcaccctgaa gttcaaaaac
     1141 ggggagcaga tcccctaacg gcggcgcctc cgcccacatt cgagtgaggg gtctggatcc
     1201 atcggggcgc aaaatccctg ccgccaaaaa ggcgctcccc cgcctggagg ccgggaagcg
     1261 ttttttcgcc ctttgcggca ttctagccgc gggaacgggc caggcatgga cccaaacgcc
     1321 gcccgcaccg gagcaacccg actggaacgc cccgccctcg gagacgcaat ccccctcccc
     1381 gaacgccgac aacccctcga ccccgtcgat ggcttccgaa gaaaccctcc ccggagccgc
     1441 cgattcgcca tcgataactg tctcgaagga aaatgcggga tcggaagccg ccccgccgcc
     1501 ggcgccctcc gagccggcgg aagtcggcgt gccaaaccgg aataattttt tcctgccgga
     1561 tttcgactct tacgggcagt ctttcttcga gcgcgagggt ttgaaattcc gcgccgggcc
     1621 ggtgcatttt cggatgagtc tcggggtcgg cctcgaatac aacgacaata tcttcggaag
     1681 ctacctcgac ccggtgtccg acacgatcgc ccatgtcacc ccgagttttc aattcgggat
     1741 cggggacttc aacgccaagg cggaagaata ccttttgctg aaataccgcc cctcgttcga
     1801 ctattatcaa agccagaccc aacaaaaccg cgtcaaccaa aacctcgaga ttaccggtca
     1861 gaccactttt tcgcgctatt cgacggccct cgatttaaac tttcaaacga acagttcccc
     1921 caacgcgacc cagagtggcg gccaggaata cagcacgttg aatttcaact gggacaatcg
     1981 gtattatctc ggatcaaaaa ccttcgcccg ggcattggtc ggcgcgaaca ttcaacagtc
     2041 tgaaaacgac aataattatc agacattctc gatcagccct caggtcggtt acgaatacag
     2101 ccccaaaacc acgctattct tcggccccta tgcgggcatt tcctatgtcg ggaatggagg
     2161 ctcccagacc ttccagggtc tgacggtcgg aatcaattat tcaaacctgc gcaaattgaa
     2221 attctccggc acttttggag ttcaagcccg acaatacgac ggccaggata cgacgggagc
     2281 gagcaatttc atcactccgg ttttcaatct tgggatgact tatgatgcga cggaaaccac
     2341 gagcctccga ctcatgctgc tgcgcgacgt gcaactctcc gatgtgcaga gggggttga
//
LOCUS       DATDAY010000193         4393 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_1307392, whole genome shotgun sequence.
ACCESSION   DATDAY010000193 DATDAY010000000
VERSION     DATDAY010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 4393)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4393)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4393
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1307392"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            129..932
                     /gene="trpA"
                     /locus_tag="VIS74_03795"
     CDS             129..932
                     /gene="trpA"
                     /locus_tag="VIS74_03795"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HEY5792395.1"
                     /translation="MANRLDATFARLRQDARKGFIAYLTAGDPDLPRSLEAARALARA
                     GADVIELGVPFSDPLADGPTNQAAAQRALEAGTTVVKLLAAIREFRRDCDVPLVLFTY
                     LNPVFTYGFERFHADAAAAGVDGILILDLPPEEEALSESFSHRHGLRSIRLIAPTTPP
                     ERMERIARRAEGFIYYVSREGVTGEQTQLADTIAGQVAEIRRHTDLPIAVGFGISTPE
                     QAAEAARSADAVVVGSAIVRRVGEFGRTASLAAKIESFTRPLVEAVKSV"
     gene            954..1766
                     /gene="dapF"
                     /locus_tag="VIS74_03800"
     CDS             954..1766
                     /gene="dapF"
                     /locus_tag="VIS74_03800"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HEY5792396.1"
                     /translation="MELSFTKMNGAGNDFVLLDNRSGALQLSREQIARICDRHRGVGA
                     DGLLLVESPANASDFRMRYYNSDGGEAEMCGNGARCFTRFALSLQADADSATSFETEA
                     GVIQARLIGAQVELEMSEPHSLAAATELEVNGRSLTVHFINTGVPHAVVFVDDLEAVD
                     IKKDGAAIRYHGHFAPKGTNANFVQVLAPGQIRIRTYERGVEDETLACGTGVVACSLI
                     HYLRDGAASPISVQVRGGDTLQVGFQASSGKFQHVTLLGPADFTFQGTIRVE"
     gene            1795..2673
                     /gene="dapA"
                     /locus_tag="VIS74_03805"
     CDS             1795..2673
                     /gene="dapA"
                     /locus_tag="VIS74_03805"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HEY5792397.1"
                     /translation="MRIQGTYTALVTPFRDGAFDRPAFIQLIESQIDAGITGVVPVGT
                     TGESPTVSHAEHVEIIRVAVEVAAGRCQVIAGTGSNSTDEAISLTQEAEKLGADAALI
                     VAPYYNKPSQEGLYRHYRKIAESVKFPIVLYSIPGRCGVEIGVETTRRLAADCPNIGS
                     IKEAGGSVERVNQLRQALPENFTIISGDDSLTLPFLSAGAVGVISVASNLIPAEVGAL
                     VQAWLEGNPQKAQEIHRHFYPLFKDLFIEPNPVPAKYLLSRQGKMTEEVRLPLCEISE
                     AARQQLTRTAQALHLI"
     gene            2726..3424
                     /gene="dapB"
                     /locus_tag="VIS74_03810"
     CDS             2726..3424
                     /gene="dapB"
                     /locus_tag="VIS74_03810"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HEY5792398.1"
                     /translation="MGRALVESSKNIDNLEIIGEIGVNDKITDFIDKCDSVIDFTHAD
                     VTASTVEVCVAARKALVIGTTGQTEAVREQIRAGAQAVPIVFAPNFSVGVNTLFWLTR
                     KAAEILGSDFDLEVVEMHHRLKKDSPSGTARRLAEILAEVRHLEYAQAIRHGREGMVG
                     ERSSAEIGMHAIRGGDVIGDHTVIFATPGERLELTHKASNRNAFAVGALRAAQWSVRQ
                     PAGLYDMQDVLGLK"
     gene            3444..3932
                     /gene="folK"
                     /locus_tag="VIS74_03815"
     CDS             3444..3932
                     /gene="folK"
                     /locus_tag="VIS74_03815"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HEY5792399.1"
                     /translation="MRAGIGLGSNQGERFQILQKAIESLHELSAAPSLDKISSVYETR
                     PVNCAPGTPAFLNAVMEIETQDAPLALLAKLQALEIAAGRPAIREKNRPRSLDLDLLY
                     VDNLALNSENLQLPHPFIFERQFVLVPLSEICPELVLPGQNRPVKSFLESEEFKEVSI
                     YK"
     gene            4035..>4393
                     /locus_tag="VIS74_03820"
     CDS             4035..>4393
                     /locus_tag="VIS74_03820"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: protein motif:HMM:NF014593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HEY5792400.1"
                     /translation="MTAYSYPFARLLDETELDVLLVGDSLAMVEMGQNDTVDTRLEDM
                     IHHVKMVRRAVTRTLLVADLPALSYETPEMAEKTALLLMEAGAEAVKLEGGVEKRAVI
                     AHLTSRDIPVMAHLGMLP"
BASE COUNT          878 a         1316 c         1270 g          929 t
ORIGIN      
        1 cgagcccggc gatcgccgcg cttccgtggc ttggcgccgg gtggtttttg tccccgcctc
       61 gccgtttttt gaaaatgaaa tggccttttt tcttcagaca ggcatgcttt cgtccctcgc
      121 aaaccgccat ggccaaccgt ctcgacgcca cctttgcccg cctgcggcag gatgcccgca
      181 aagggtttat cgcctacctc acggcgggag atcccgactt gccgcgctct ctggaggccg
      241 cccgggccct ggcccgggcc ggcgcggacg tcatcgagct gggcgtgccc ttttccgatc
      301 ccctggccga cggccccacc aaccaggcgg cggcccagcg cgccctggaa gccggaacca
      361 ccgtcgtcaa attattggcc gccatccgcg aattccgccg ggattgcgac gtgccgctgg
      421 tgcttttcac ctatttaaat ccggtgttca cctacggctt cgagcgtttt catgccgatg
      481 cggcggcggc gggcgtggat ggcatcctga ttcttgacct gccgccggag gaggaggccc
      541 tcagcgagtc gttttcccat cggcacggcc tgcgttcgat ccggctcatc gcgcccacca
      601 ccccgccgga gcgaatggag cgcatcgccc gccgcgcgga ggggtttatt tattacgtct
      661 cccgcgaggg cgtcaccggc gagcaaaccc agctggcgga taccatcgcc ggccaggtcg
      721 ccgaaatccg ccggcacacc gatctgccaa tcgccgtcgg ttttggcatt tccacgcccg
      781 aacaagcggc ggaagcggcc cgcagcgccg atgccgtggt ggtcggcagc gccattgttc
      841 gccgggtcgg cgagtttggc cggacggcca gcctggcggc gaaaatcgaa tcctttaccc
      901 gcccgcttgt cgaagcggtg aaatccgtgt aagttgctac tccttttcag ccaatggaac
      961 tttccttcac caaaatgaac ggcgccggca atgactttgt cctgctggac aatcgctcgg
     1021 gcgcgcttca gttgtcgcgc gaacagatcg cccgcatctg cgaccgccat cgcggcgtcg
     1081 gcgcggacgg ccttttgctg gtggaatccc cggccaatgc cagcgatttt cgcatgcgtt
     1141 attacaacag cgacggcggc gaagccgaaa tgtgcggcaa tggtgcgcgc tgttttaccc
     1201 gttttgcgct gagcctccag gcggacgcgg attcggccac ctcttttgag acggaagccg
     1261 gcgtcatcca agcccgcctg attggcgccc aggtggaatt ggaaatgagc gaaccgcaca
     1321 gcctcgcggc cgccacggaa ctggaagtca acggccgttc cttgacggtt catttcatca
     1381 acaccggtgt tccgcatgcg gttgtttttg tggatgattt ggaagcggtt gatatcaaga
     1441 aggatggcgc cgccattcgt taccacggcc acttcgctcc caagggaacg aatgccaatt
     1501 ttgtccaggt gctcgctccc gggcagattc gcattcgcac ctatgaacgc ggcgtggaag
     1561 acgaaacgct ggcttgcgga actggcgtgg ttgcctgctc gttgatccat taccttcggg
     1621 acggcgccgc ctcgccgatt tccgtccaag ttcggggtgg cgacaccctt caagtcgggt
     1681 ttcaagcctc ctctggaaag ttccagcacg tgacgctgct cggaccggcg gatttcacct
     1741 tccagggaac cattcgggtg gaataatccc ctccgggtta gaaactattt tccgatgcgc
     1801 attcaaggca cctataccgc gctggtcacc ccgtttcgag acggagcatt tgaccgtccc
     1861 gcatttattc aactcattga atcacagatt gatgcgggaa tcactggcgt tgttcccgtc
     1921 ggcacgaccg gggaatctcc caccgtcagc catgcggaac acgtggaaat tatccgggtg
     1981 gccgtcgaag tggcggcggg ccgctgccag gtcattgccg gcaccggctc caattccacg
     2041 gatgaggcga tttccctgac tcaggaagcc gaaaagcttg gcgcggacgc ggctttgatt
     2101 gtggcgccct attacaacaa gccgtcccag gaagggcttt accgccatta ccggaaaatt
     2161 gccgaatcgg taaagtttcc gatcgtgctc tacagcattc ccggccgttg cggcgtcgaa
     2221 atcggcgtgg aaaccacccg ccggctggcg gcggattgcc cgaatattgg gtccatcaag
     2281 gaagccggcg gcagcgtgga acgcgtcaat cagctccgcc aggcgctgcc ggagaatttt
     2341 accatcattt ccggcgacga ttccctgacg ctgcccttcc tcagcgcggg cgcggtggga
     2401 gtgatcagtg tcgcctccaa cctcattccc gccgaagtcg gcgcgctggt ccaagcttgg
     2461 ctggaaggaa atcctcaaaa ggctcaggaa atccatcggc atttttaccc gcttttcaag
     2521 gatttgttca ttgaacccaa cccggttccg gccaaatacc tgctcagccg gcagggaaaa
     2581 atgacggagg aagtccgcct gccgctttgc gaaataagcg aagcggcccg gcaacagctc
     2641 acccgaacgg cgcaggctct ccatttgatt taagaccatg aactctcatc cgacccaagt
     2701 catcattcgg ggagcccatg gaaggatggg gcgggcgctt gttgaatcat cgaaaaatat
     2761 cgataatctg gaaatcattg gtgaaattgg agttaacgat aaaattacgg attttattga
     2821 taaatgcgat tccgtcatcg atttcactca tgccgacgtc accgcctcca cggtggaggt
     2881 ttgcgtggcg gcgcgcaagg cgttggtgat cggaacgacc ggccagaccg aggcggttcg
     2941 ggagcagatt cgggccggcg ctcaggctgt cccgattgtc tttgctccca atttcagcgt
     3001 gggcgttaac accctcttct ggctgacccg aaaggcggcg gaaatcctgg gatccgattt
     3061 cgacttggaa gtggtggaaa tgcaccatcg cctcaaaaaa gactccccca gcggcaccgc
     3121 gcgccgcctg gccgaaattc tcgcggaagt ccgccatttg gaatacgccc aggcaatccg
     3181 gcatggccgc gagggcatgg tgggcgaacg ctcctcggcg gaaatcggca tgcacgccat
     3241 tcgcgggggt gacgtcattg gcgatcacac ggtgatcttt gcgacgcccg gcgagcgatt
     3301 ggaactcacc cacaaagctt cgaatcggaa tgctttcgcc gtgggagcgc ttcgcgccgc
     3361 ccaatggagc gtccgtcagc cagcgggact ttacgacatg caggatgtcc ttggcctgaa
     3421 ataggccgcc aatcatcaaa ctcatgagag cgggaatcgg cttgggttcg aatcagggag
     3481 agcgatttca gattctgcaa aaagcgatcg agtccctgca cgaactgtcc gcggcccctt
     3541 cgctggacaa gatttcctcc gtttacgaaa cccgcccggt caactgcgcg ccgggaacgc
     3601 cggcttttct caatgccgtc atggaaatcg aaacccagga tgctcccttg gccctgctgg
     3661 ccaaacttca ggcattggaa atcgccgccg gaaggccggc gattcgggag aagaacaggc
     3721 cgcgctctct ggatctcgat ctgctgtatg tggacaattt ggcgcttaac tctgaaaatc
     3781 tgcagctccc gcatcccttt attttcgagc ggcagtttgt cctggtcccc ctttccgaaa
     3841 tctgccccga actggttttg cccggccaga accgtcccgt aaaatcgttt ctcgaatcgg
     3901 aggaattcaa agaagtttca atttataaat aacttataaa aagtaacata gtaccatttt
     3961 tacgatttaa tttgaaaaac aaatctccca atcaatttgc caagcttaag caaaccggcc
     4021 acaaattaac cgcgttgacg gcttattcct acccattcgc gcgtttgttg gacgaaacgg
     4081 aattggatgt tcttctggtc ggcgattctt tggccatggt ggagatgggc cagaatgaca
     4141 cggttgatac gcggctggaa gacatgattc atcatgtcaa aatggtgcgg cgggcggtca
     4201 cgcggaccct gctggtggcg gacttgccgg cgctctctta cgagactccg gaaatggccg
     4261 agaagaccgc gcttctgctg atggaagccg gagccgaggc ggtcaagctg gagggcggag
     4321 ttgaaaaacg agccgtgatt gcccatctga cgagccggga tatcccggtc atggctcact
     4381 tgggaatgct gcc
//
LOCUS       DATDAY010000194         2865 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_1273393, whole genome shotgun sequence.
ACCESSION   DATDAY010000194 DATDAY010000000
VERSION     DATDAY010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2865)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2865)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2865
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1273393"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(63..440)
                     /locus_tag="VIS74_03825"
     CDS             complement(63..440)
                     /locus_tag="VIS74_03825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792401.1"
                     /translation="MPLLSIVFGALLDLLGAYGFVSTGGTHYTSLIPSAFGTLLILCG
                     LLALALPSARKHFMHAAATVGLLGTLGGLGMGLPKLSALLAGAAARPSAVVLQILMGV
                     ISLVFVALCVRSFILARRAKPVS"
     gene            544..1329
                     /locus_tag="VIS74_03830"
     CDS             544..1329
                     /locus_tag="VIS74_03830"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEY5792402.1"
                     /translation="MSLKAILFDLDDTLVVDEAVSKQTFAAIGQLAARHGAEGDRLQR
                     VAQSTARKLWGEGSCHAYCRSIGISAFECLWGKFEGATPELTALREWSHAFRVEAFDR
                     ALREQLIDNPVLAQELAAAWSSTRRKNQRLMPDAVETLARLSAYRIGLLTNGAPDLQR
                     EKLAASGLGSFFQAVAVSGEHGIGKPKPEIFQRLLAELGVEAGEAVMVGNSLERDIAG
                     ANAAGITSIWLKVAGSEEHFETTPRFTLHGLAELPKILAGLEA"
     gene            1531..>2865
                     /gene="bamA"
                     /locus_tag="VIS74_03835"
     CDS             1531..>2865
                     /gene="bamA"
                     /locus_tag="VIS74_03835"
                     /inference="COORDINATES: protein motif:HMM:TIGR03303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HEY5792403.1"
                     /translation="MRIFSRCALPGAIALTLAAFAPLHAQTPQQAPIVREIAIEFVGP
                     EAITRDRVLANLATKVGQPYSQFDIEQDIRALYATGVVSNVRMFGEPAPDGVKVTVLL
                     QGKPSIQEILIEGTGQIAMNRVRKEIGSKVGDPVSEERLEDDRQKILKLYEDRNYTDV
                     DVQYVVKDVPNTGLVSVIYSINEGPKMVVKRISFAGNDSILPRDLAKAMKTKKQDWLT
                     IFNKSGRLLPANEEEDKAAVRSLYQNRGFADAQVLGYDVQPLKGTGVELVISVQEGIQ
                     YHVNSVAFEGVAVASPEELKSRLAMVDGSLYTPSGMTKDLKGLRDYYGVRGYVDMMAL
                     PEVLPAGPGAVDITYRVDEGVQSYVNLVNIQGNTRTRDHVIRRELAIKPGEIFDTTRV
                     DLSKERLNNLNYFSKVDAIPADTLVPGRKDLNIIVEEKRTGSFNFGAGFSSID"
BASE COUNT          624 a          896 c          866 g          479 t
ORIGIN      
        1 gatttcataa atcgaaggtt cgggtgaagg ttttcaaggg aaggaatcgt ggggcggcgc
       61 aatcaggaaa ccggcttggc ccggcgggcc agaatgaacg aacgcacgca aagcgccaca
      121 aaaaccaggc tgatgacgcc catgaggatc tgcaggacga ccgcggacgg ccgggcggcg
      181 gcgccggcca gcaaggcgct caacttgggc agccccatgc ccagtccgcc cagagttccg
      241 agcaggccca ccgtggcggc cgcgtgcatg aagtgcttgc gggcggacgg gagcgcgagg
      301 gccagaaggc cgcagagaat caagagggtt ccaaaggccg agggaatcag cgaggtataa
      361 tgagttcccc cggtggaaac gaatccgtaa gctccgagca gatcgagcaa agcgccaaag
      421 acaatggaaa gaagcggcat ggttgaaaca gatttaggtt ggtaagtttg aaattttaag
      481 caaatagatg ccgtccgtcc ggcaccactt caaacttacc aacctcaaac ttcaaacttt
      541 ttcgtgagcc tgaaagctat tttgttcgac cttgacgaca cgctggtcgt ggatgaagcc
      601 gtctccaaac aaaccttcgc cgccatcggc cagctcgccg cccggcatgg cgccgagggg
      661 gaccggctcc agcgcgtcgc ccagtccacc gcccgcaaac tctggggcga gggctcgtgc
      721 cacgcttatt gccggagcat cggcatcagc gcctttgaat gcctctgggg aaaatttgaa
      781 ggcgccaccc cggaactcac cgcgctgcgg gaatggtcgc acgccttccg cgtcgaggca
      841 ttcgaccgcg cgctgcggga acaattgatc gacaacccgg ttctggccca ggagctcgcc
      901 gcggcctggt cgagcacccg gcgcaaaaac cagcggttga tgccggacgc ggtggaaacc
      961 ctcgcccggc tctcggctta ccggatcggc ctgctcacca acggcgctcc ggacctgcag
     1021 cgcgaaaaac tcgccgcttc gggcctgggc tccttctttc aggcagtggc ggtctccggc
     1081 gagcacggca tcggcaaacc caaaccggaa atctttcagc gcctcctcgc cgaactcggc
     1141 gtcgaggcgg gcgaggccgt catggtcgga aacagcctcg aacgcgacat cgccggcgcg
     1201 aatgccgccg ggatcacgag catctggctg aaagtggccg gatcggaaga gcatttcgaa
     1261 accacgccgc gctttaccct ccacggattg gcggaactgc cgaagatttt ggccgggctg
     1321 gaagcctgaa tcaccgccga aacgcggaga cgccgaggcg ggaagcatcc aaacagggct
     1381 tgaaaatggg ttttctccgc gcccccgcgt cccggcggtg aatttaccca atccttcagc
     1441 ccccatacgg ggaattcgta gcttgacctt tggcggccaa agccgcatca aatgcccgat
     1501 tccgaatttt aactaagttt gcccaaccga atgaggattt tttcacgctg cgcccttccg
     1561 ggagccattg ctttgactct ggcggccttt gcccccctcc acgcccagac cccccaacag
     1621 gcgccgatcg tccgggaaat cgccattgaa tttgtggggc cggaggccat cacccgcgac
     1681 cgcgttctcg ccaatctggc caccaaggtc gggcagcctt attcgcaatt cgacatcgag
     1741 caggacatcc gcgcgctcta cgccaccggc gtggtcagca acgtccgcat gttcggcgaa
     1801 ccggcgccgg atggcgtcaa ggtcaccgtc ctgctgcagg gcaagccctc cattcaggaa
     1861 atcctcatcg agggcaccgg ccagatcgcg atgaatcgcg tgcgcaagga gatcggctca
     1921 aaggtgggcg atccggtcag cgaggagcgg ctggaggatg accgccagaa gattctcaag
     1981 ctttacgagg accgcaacta caccgacgtg gacgtccagt atgtcgtgaa ggatgtcccc
     2041 aacaccgggc ttgtcagcgt catttactcc attaacgaag gtccgaagat ggtggtcaaa
     2101 cgcatcagct tcgccggcaa tgactcgatc cttccccgcg acctggccaa ggccatgaag
     2161 accaagaagc aggactggct gacgatcttc aataagagcg ggcggctgct gccggccaac
     2221 gaggaggaag acaaggccgc ggtccgcagc ctgtatcaaa accgcggttt tgccgacgcg
     2281 caggtgctcg gctacgacgt ccagccgctc aagggaaccg gagtcgagct ggtcatcagc
     2341 gtccaggaag gcatccaata ccacgtcaac tccgtcgcct tcgaaggcgt ggcggtagcc
     2401 agcccggagg aattgaagtc gcgcctggcc atggtggacg gctccctcta cacgccgtcg
     2461 ggcatgacga aggacctcaa aggcctgcgc gactactacg gcgtgcgcgg ttatgtggac
     2521 atgatggcgc tgcccgaagt cctgcccgcc ggccccggcg cggtggacat tacctaccgc
     2581 gtggatgaag gcgtccagtc ctacgtgaat ctcgtcaaca tccagggcaa cacccgcacg
     2641 cgggatcacg tcatccggcg tgaattggcc atcaagcccg gcgaaatctt cgacacgacc
     2701 cgggtggatc tgagcaagga gcggctgaac aacctgaatt atttctcgaa ggtggacgcg
     2761 attcccgccg acaccctggt gccgggccgc aaggatttga acatcatcgt cgaggaaaaa
     2821 cgcacgggtt ccttcaactt cggcgccggc ttcagcagca tcgac
//
LOCUS       DATDAY010000195         6088 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_2179861, whole genome shotgun sequence.
ACCESSION   DATDAY010000195 DATDAY010000000
VERSION     DATDAY010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 6088)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6088)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6088
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2179861"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..309
                     /locus_tag="VIS74_03840"
     CDS             <1..309
                     /locus_tag="VIS74_03840"
                     /inference="COORDINATES: protein motif:HMM:NF028184.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH36 C-terminal domain-containing protein"
                     /protein_id="HEY5792404.1"
                     /translation="DLYRLETAAEAGFSIWQTVLPDGSRSVYSFVSLGQLQGIHHAAP
                     RLRGLVPGAVYAVSDDLEKELGRYPGWQLMSLGLPGDDAFGGMGRAIRSRTLLLEKVA
                     "
     gene            351..2303
                     /locus_tag="VIS74_03845"
     CDS             351..2303
                     /locus_tag="VIS74_03845"
                     /inference="COORDINATES: protein motif:HMM:NF012686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792405.1"
                     /translation="MKLIDWLLIALPIAIVVGIALYTRRYVRSVTDFMAGGRCAGRYL
                     LSTAGSEMTAGAALFVATLEQFSVTGFTIQWWSQILTPVGLLLAITGFVGYRYRQTRA
                     LTLGQFFEMRYSRNFRLYAGVLGFLAGIINFGVIPAVEARFFVHFLNLPATLPIFSLA
                     IPTDLLLMTLFLALAVLLPMVGGQVTMLVVGCAEGMISQIFYVIIAIVMVSLVTWSQM
                     SQVLMAQPAGHSMVNPFDTFQAQDFNLWFVLMLAFLNIYGTMAWQKSQAFNASPITPH
                     EGRMGGILYRWRYFASGTMMLILSLCTLTFMQHPDFAAGAAHAKELTDAIPNAKVADQ
                     MDWPIALSLLLPAGIKGMLCAIVLMGLIASNGMAMHSWGGIFIQDVVVPLRKTPLTTA
                     QHLLVLRLAIFGVAIFAFIFGALFQQTDKLLLWWALTQAIFVGGAGAAIIGGLYWKRG
                     TAAGAWSGMLTGSILSVGGIIFRQVYPEVPVNGMQISFFAALIAIVVYVTVSRLTSRE
                     PHDMDRLLNRGKYAVEAEDSGHAIQKTSWLTTIIGIDKHFTRSDRWITYGISGWSYFW
                     FFLFIFGSAVYLIHPWSNEAWLSYWHISNIILPLIIAVLTTIWFTIGGVVDLRKFFQR
                     LRAERVDAADDGTVSAEDTGKPESAK"
     gene            2300..3340
                     /locus_tag="VIS74_03850"
     CDS             2300..3340
                     /locus_tag="VIS74_03850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /protein_id="HEY5792406.1"
                     /translation="MSEPFINRLRPAPKNGGFRMDDYWVWCGSVVKAGNPDEDGRYHL
                     FASRWPRALTFAGHWITNSEIVRAVSDTPEGPYRFEEVVLPWRHRKYFDGLVTHNPSI
                     KKFGGKYYLFYIGITYDFDIPTPERQIYEGKYAEHRELYRLAWDNKRIGLAVSDSVAG
                     PWKRPALPLIEPRPGKWDARITSNPSAAIRDDGHTVLIYKSKGEGNTPFQLGIATAPH
                     PEGPYTRASDEPIFPFDCEDPFLWWEGGRYHVIMKDFKGELCGEVNAGAYAWSEDGIH
                     WNLPEDPRAYSRTIRWDDGTVSTHGNFERPNLLIENGTPTHLFAAISDGPLPHWQSQS
                     TYNVCIPLAPAG"
     gene            complement(3400..4530)
                     /locus_tag="VIS74_03855"
     CDS             complement(3400..4530)
                     /locus_tag="VIS74_03855"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEY5792407.1"
                     /translation="MSAEDSPSISLLEKAFDRPWDQQLPLHARVNRGLRYAIENFFED
                     GQRFFTEKELIENLKVSQVTVRRAVYDLVREGLLERRVAKGSFVRKRAAESRGQPFEV
                     AVHLADWDSSFLNQTLRQVLDACGSKRRETKVFYSRQSGTILSQEGEQWNQAEISGAI
                     LIANPTDLAKDLSRLYENAGKPVVAIDSKAPGEYAHFVGTDNQATIRLGLDHLTQLGH
                     RRICLLLNEPEAHENVRLRARYFEEECQKRNLPDCPVIHCGTQFWENSYEKAYESMPK
                     VMELNPAPTAIFAFDDPGAWAVLKWCSQHGVKVPQELSVLGFANDKPSAFVQPALTTI
                     AHPIAEIASMAVDLLTLKSGKGQVRLLEPSLVVRESTGPVRS"
     gene            4718..5038
                     /locus_tag="VIS74_03860"
     CDS             4718..5038
                     /locus_tag="VIS74_03860"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HEY5792408.1"
                     /translation="MGAQNFLGPDIVLEGDIHTSGNLIFDGTLKGSITGENSLTIGSS
                     ARIEGRLTAQSLTIHGRVTGDVMAIDLAELKGGSALHGDVVARRLRMEEGVVFIGNAK
                     VSPK"
     gene            complement(5152..5619)
                     /locus_tag="VIS74_03865"
     CDS             complement(5152..5619)
                     /locus_tag="VIS74_03865"
                     /inference="COORDINATES: protein motif:HMM:NF024230.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HEY5792409.1"
                     /translation="MSLLHLANQVIFGGIAAAGFGILFNCPPRIIGWCFASGAVALAV
                     RTVALDGGFSLPAASFFAALVVAGTDRAWRRAQSVRGSVIAVVGCIPMIPGSLAAKGL
                     THLFLLFEASPSEGILPLTIALESLVIVATTFAAIGTALAIPALIFPFRQVET"
     gene            complement(5621..>6088)
                     /locus_tag="VIS74_03870"
     CDS             complement(5621..>6088)
                     /locus_tag="VIS74_03870"
                     /inference="COORDINATES: protein motif:HMM:NF018448.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HEY5792410.1"
                     /translation="ESARAGLDRIPRDAGAYPMLFVCGATGLACAAFGQLLGAPPLAI
                     PPVFLGAAAGQYLRQAMLARKMNLFFMVCLVSFCSSLISGLLARMLGLANLETTAVAA
                     VLLLVPGPAILNAQMDAIDGRPSLAAARALRVVFILLFMTLGFYLSQQMISLF"
BASE COUNT         1189 a         1995 c         1727 g         1177 t
ORIGIN      
        1 gatctttacc ggctcgaaac cgccgccgag gcaggcttct ccatctggca gaccgtgctg
       61 ccggacggca gccgctccgt gtattccttc gtttccctcg gccagttgca gggaatccat
      121 cacgcggccc cgcgcctgcg cggcctcgtc cccggagcgg tgtatgcggt gagcgacgac
      181 ctcgaaaagg aactcggccg ttatccgggc tggcagttga tgtcgctcgg gctgccgggc
      241 gatgacgcct tcggcggaat gggccgggcc attcgcagcc gcacgttgct tctcgaaaaa
      301 gtcgcctaat cgcctcactc tcaaaaacct gatcccatcc aaaccgttcc atgaaattaa
      361 ttgactggct gctcatcgcg ctgcccatcg ccatcgtcgt cggcatcgcc ctctacaccc
      421 gccgatacgt ccgcagcgtc accgacttca tggccggcgg acgctgcgcc ggacgttacc
      481 tgctctccac ggcgggcagt gagatgaccg ccggcgccgc gctctttgtc gcgacgctcg
      541 agcaattttc ggtcacgggg ttcacgatcc aatggtggag ccagatcctg actcccgtcg
      601 gcctgctgct ggcaatcacg ggatttgtgg gctaccgcta ccggcagacg cgcgcgctga
      661 cgctcggcca gtttttcgag atgcgctaca gccggaattt ccggctctac gccggcgtgc
      721 tgggattcct ggccggcatc atcaacttcg gcgtcattcc ggccgtcgaa gcgcggtttt
      781 tcgtccattt tctcaacctg ccggcgacgc tgcccatttt ctcgctggcc atcccgaccg
      841 atctgctgct gatgacgcta ttcctggcct tggcggtgct gctgccgatg gtaggcggac
      901 aggtcacgat gctcgtggtg ggctgcgccg agggcatgat ctcgcagatt ttctacgtga
      961 tcatcgccat cgtcatggtc tccctcgtga cgtggagtca aatgagccag gttctgatgg
     1021 cccagcccgc cgggcattcg atggtcaacc ccttcgacac cttccaggcc caggatttca
     1081 atttgtggtt cgtgctcatg ctggccttcc tcaacatcta cgggacgatg gcctggcaga
     1141 agtcccaggc gtttaacgcg tcccccatta ctccgcacga gggccggatg ggcggcattc
     1201 tttaccgctg gcgctacttt gccagcggga cgatgatgct catcctctcg ctctgcacgc
     1261 tgaccttcat gcagcatccg gacttcgcgg ccggagcggc ccacgcgaag gaactcaccg
     1321 acgccattcc caacgccaag gtcgccgacc aaatggactg gccaatcgcc ctctccctcc
     1381 tgcttcccgc gggcatcaaa ggaatgcttt gcgcgatcgt cctcatgggg ctgatcgcca
     1441 gcaacggcat ggccatgcac tcgtggggcg gaattttcat ccaggacgtg gtcgtgcccc
     1501 tgcgcaagac gccgctgacg accgcccagc acctgctcgt cctccggctc gccatcttcg
     1561 gtgtggccat tttcgccttc atctttggcg cgctcttcca gcaaaccgac aagctgctgc
     1621 tgtggtgggc gctcacccag gccatcttcg tgggcggcgc gggcgcggcc atcatcggcg
     1681 gcctctattg gaagcgaggc acggcggccg gcgcctggtc gggcatgctc accggttcca
     1741 tcctctcggt cggcggcatt attttccggc aggtgtatcc cgaagttccg gtcaacggca
     1801 tgcagatttc cttcttcgcc gcgctgatcg ccatcgtggt ttatgtgacg gtctcgcggc
     1861 tcaccagccg cgagccgcat gacatggatc gcctgttgaa ccgcggaaaa tatgccgtcg
     1921 aggcggagga ctcgggccat gccatccaaa aaacctcctg gctcaccacg attatcggca
     1981 tcgacaagca tttcacccgg tcggaccgct ggatcacata cggcatttcc ggatggagct
     2041 atttctggtt tttcctcttc atcttcggct ccgccgttta cttgatccat ccgtggtcga
     2101 atgaagcctg gctctcctac tggcacattt cgaacatcat cctcccgctc atcatcgcgg
     2161 tcctcacgac catctggttc acgatcggcg gcgtggtcga tctgcggaaa ttcttccaac
     2221 ggctccgcgc cgagcgggtg gacgcggctg acgacggcac ggtctcggcg gaagataccg
     2281 gcaagccgga gtccgccaaa tgagcgaacc cttcatcaat cgcctccggc ccgcgcccaa
     2341 gaacggcggg ttccgcatgg acgattattg ggtctggtgc ggttcggtcg taaaggccgg
     2401 caatccggat gaggacggcc gctaccatct gttcgcctcc cgctggccgc gggcgctgac
     2461 cttcgccggg cattggatca cgaattccga gatcgtccgc gccgtctcgg acacgccgga
     2521 gggcccctat cgtttcgagg aagtggtgct gccctggcgg caccggaaat attttgacgg
     2581 cctggtcacg cacaatccct cgatcaaaaa attcggcggg aaatattacc tgttttacat
     2641 cggcatcacc tacgatttcg acatcccgac gccggagcgc cagatttacg agggcaaata
     2701 cgcggagcac cgcgagctct accgcctggc ctgggacaat aaacgcatcg gcctcgcggt
     2761 gagcgactcc gtggccggtc cgtggaaacg ccccgcgctt ccgctcatcg aaccgcgccc
     2821 cggcaaatgg gatgcgcgca tcacgagcaa cccgagcgcg gccattcgcg acgacggcca
     2881 cacggtgctc atctacaaat ccaagggcga aggcaatacg cccttccagc tcggcatcgc
     2941 caccgcgccg catccggagg ggccttacac ccgcgcgtcg gatgagccga ttttcccctt
     3001 tgactgcgaa gatccgttcc tctggtggga gggcggccgt tatcacgtca tcatgaagga
     3061 cttcaagggc gagctctgcg gcgaagtcaa cgccggcgcc tacgcgtgga gcgaggacgg
     3121 cattcattgg aacctgcccg aggatccccg cgcttactcg cggacgatcc gctgggacga
     3181 cggcaccgtc tccacccacg gcaacttcga gcgcccgaat ctgctgatcg aaaacggaac
     3241 gcccacgcac ctgtttgccg ccatcagcga cgggccgctc ccccactggc agtcgcaaag
     3301 cacctacaac gtctgcatcc ccctcgcccc ggcgggttag ggagattccc ggaccaagcc
     3361 agagccgaga tttggaccgc cgtcacggat tcgcgcggat tacgagcgaa ccgggccggt
     3421 gctttcgcgc acgacgaggc ttggttcgag caagcggacc tggcctttgc cggatttcaa
     3481 ggtcaggaga tccaccgcca tcgacgcgat ttcggcaatc ggatgagcaa tggttgtcaa
     3541 agcgggctgc acaaacgcac tcggcttgtc attggcaaaa ccgaggaccg acagttcctg
     3601 gggcaccttc accccgtgct gcgaacacca cttgagcacg gcccaggccc ccggatcgtc
     3661 gaaggcaaag atggcggtcg gcgccggatt gagctccatc actttcggca tgctctcata
     3721 ggccttctcg taggagtttt cccagaactg cgtgccgcaa tggatcaccg ggcaatcggg
     3781 gagattgcgc ttttggcact cctcctcgaa ataccgggcg cgcaaccgga cgttttcgtg
     3841 cgcttcgggt tcattgagca gcaggcaaat gcgccgatgg ccaagctggg tcaagtgatc
     3901 aagccccagc cggatcgtcg cctgattatc cgtgcccaca aaatgggcgt attcgccggg
     3961 cgccttgctg tcgatggcca ccaccggctt gcccgcgttc tcgtaaaggc ggcttaagtc
     4021 cttcgccaga tccgtgggat tcgcgatcag gatcgctccg gaaatttccg cctggttcca
     4081 ttgctcccct tcctggctga ggatggtccc ggactggcgg gaataaaaca ccttcgtctc
     4141 ccggcgcttc gaaccgcagg catcgaggac ctggcggaga gtttgattta aaaacgacga
     4201 atcccaatcc gccaaatgca cggcgacttc aaagggctgc ccgcggcttt ccgccgcccg
     4261 tttgcgcaca aaactgccct tggccacccg ccgctccagc aagccctccc gcaccaagtc
     4321 gtaaaccgcg cggcgcacgg tgacctggga gaccttcaga ttttcaatga gctccttctc
     4381 cgtgaaaaac cgctggccat cctcaaaaaa attttcaatc gcataacgca atccccgatt
     4441 cacccgcgcg tgcaagggca actgctggtc ccaggggcga tcaaacgctt tttccaaaag
     4501 cgaaatggag ggagagtctt ccgccgacat ggactatccg tataataatg acagaaatcc
     4561 gcaagttgaa tccagccgtc tccttccgta accgcggatt cccaagcccc attccgtaaa
     4621 aatgaccttc tctgagttga tttttatggg ttccgcagtc tagctttgcg caatgtcgaa
     4681 accctctccc gttctcattc cttcttcccc tgttgatgtg ggcgcccaga attttctggg
     4741 gcccgatatc gttctggagg gggacattca cacttccggc aacctgattt ttgatggcac
     4801 gctcaaggga tccatcaccg gagaaaactc tctcaccatt ggttccagcg cccggatcga
     4861 aggccgtttg acggcgcaat ctctcaccat ccacggccgg gtgaccggcg acgtaatggc
     4921 aatcgatttg gcggaattaa aaggagggtc cgccctccat ggcgacgtcg tcgccaggcg
     4981 cctgcgcatg gaggaaggcg tcgtgttcat cggcaacgcc aaggtatccc ccaagtagaa
     5041 tccttctccc ttcaccctgc ccggtctccg gtaaaaggtg gggttgatca aatttacgcg
     5101 tttttaaatc tgctccaatc cgtgaaatca gcggattaaa tcagcgtttc cctaagtttc
     5161 gacctgcctg aacggaaaaa tcagggccgg gatcgccagc gcggtgccga tcgccgcgaa
     5221 agtggtcgcc acgatcacca ggctttccag agcgattgtc aaaggcagga tgccctcgct
     5281 gggcgaagcc tcgaaaagca aaaacaggtg ggttaacccc ttggccgcga gactgcctgg
     5341 aatcatcggg atgcatccga ccacggcgat gacggatccc ctcaccgact gggcgcgccg
     5401 ccaggcccgg tccgttcccg cgaccaccag ggcggcaaaa aacgaggcgg ccggcaggct
     5461 aaagccgccg tccagcgcga cggttcgcac cgcgagcgcc acggctcccg acgcaaaaca
     5521 ccacccgatg attcgcggcg gacagttgaa caaaatgcca aatcccgcgg cggcaatgcc
     5581 accgaagatc acctgatttg ccaaatgaag caggctcatc tcagaaaagg ctgatcattt
     5641 gctgggagag ataaaaaccc agcgtcatga ataagagaat gaataccacc cggagggcgc
     5701 gggcggcggc caaactcggc cggccatcaa tcgcgtccat ctgggcgttc aggatggccg
     5761 gccccggcac cagcaacaag acggccgcca ccgcggtggt ctcaagattc gccaagccaa
     5821 gcatccgggc gagcagcccg gaaatcaaag acgagcaaaa actcacgagg cacaccatga
     5881 agaagagatt catcttccgc gccaacatcg cctggcgcag atattggccg gccgccgccc
     5941 ccagaaacac cggcggaatc gccagcggag gggcgcccag caattggccg aatgccgcgc
     6001 acgccagacc ggtcgcgcca cagacaaaca gcatgggata cgccccggca tcgcgcggaa
     6061 tgcgatccag accggcccgg gcggactc
//
LOCUS       DATDAY010000196         2939 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_131611, whole genome shotgun sequence.
ACCESSION   DATDAY010000196 DATDAY010000000
VERSION     DATDAY010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2939)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2939)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2939
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_131611"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1172)
                     /locus_tag="VIS74_03875"
     CDS             complement(<1..1172)
                     /locus_tag="VIS74_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY5792411.1"
                     /translation="MKLSDLKHSGHLPTLITAFLYFDVSFMVWTLLGPLGAQIGESLG
                     LNAQEKGLMVAMPILAGAILRIVLGVTVDHIGAKKTGLIAQIIVIVGLLYAWLVGLRD
                     FQATLLMGFILGFAGASFAVALPQAGRWYPPNMQGLVMGLAGAGNIGTVLDALFAPRL
                     AQAFGWQNVFGLALIPAILVFFIYLIFSREANVEIKRKKFSDYLTLLKDRDAHWFCFY
                     YLISFGGFVGLASSYVLYFKAEFALTAVQAGDFAAICTATGALLRPVGGAVADRIGGI
                     RALFQFYTLAALALIGAAFLHSLYLNLTVLAIASAALGMANGSVFQLLPQRFGRDIGV
                     MTGLVGAAGGVGGFYLASSLGYSKGLTGNYTTGLLVFAGLCAVALVGLAMVKTRWRT"
     gene            complement(1169..2248)
                     /locus_tag="VIS74_03880"
     CDS             complement(1169..2248)
                     /locus_tag="VIS74_03880"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CmpA/NrtA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HEY5792412.1"
                     /translation="MTSAAPKPTTRKVAPIRPSQGILRLGYVPLADAAPLLMAEALGL
                     FQDSGLQVQLNRELGWGSIREKLVYGELDAAHAPAGMLYSILCGTHSPPCEVSTDLVL
                     SLQGNAITLSRRLWEKGVRDGSTLRQLLRSEAPRKPAFAVVSAFSTHPFLLRKWLRDA
                     GVNPDRDIRIVFLPPALVGEHMKAGQIDGFCVGEPWNSAATAAGDGWIVATSATLAPR
                     HPEKVLLASHSLRHQRAEEYARLRQAIVTAAQYCDTPQGREELVGVLDSRRLFPVSRE
                     VLANSLLGPLEKGVGQKPEAGPLHYFFKNDTNRATVDRGVWLLDTLADSPSVNLTPPQ
                     RRAFLDAFEDHLQTSTPPPTTKTKK"
     gene            2345..>2939
                     /locus_tag="VIS74_03885"
     CDS             2345..>2939
                     /locus_tag="VIS74_03885"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY5792413.1"
                     /translation="MALDLDIRLLRMFVTLARVGSHTRTGAVLNLTQSAISHGMKRLE
                     EQLGCALIYKKGKSAHLTPEGRHFLGQVLRILDSLDRAAESVSGRGESRTKLSVVFSA
                     AMAHAILAPVLREFRESYPNLSLNVRLEDSPAAIREVEEGKADLAVVVDSNLPAGIKP
                     HPLFKDQLQLLISPLHPWNKKDKLTVRDLRDEHFLLYQ"
BASE COUNT          550 a          889 c         1017 g          483 t
ORIGIN      
        1 gtgcgccagc gggtcttgac catcgcgagc ccgacgaggg cgaccgcgca gagtcccgcg
       61 aagaccagca ggccggtggt gtaatttccg gtcaagccct tggagtaacc cagcgagctg
      121 gcgagataaa acccgcccac gccgccggcc gcgccgacga gcccggtcat gacgccgatg
      181 tcgcgtccga aacgctgcgg cagcagctgg aagacggagc cattggccat tcccaacgcg
      241 gccgaggcga tcgccaggac ggtgagattg aggtagaggc tgtgcaggaa ggccgcgccg
      301 atgagggcga gggccgcgag cgtgtagaat tggaacagcg cgcgaatccc gccgatgcgg
      361 tcggccaccg ctccgcccac cggccgcagc aacgcgccgg tcgcggtgca gatggccgcg
      421 aaatccccgg cttgcaccgc cgtgagcgcg aactccgcct tgaagtaaag cacgtaggag
      481 ctggccagcc caacgaatcc gccgaagctg atgaggtaat agaagcagaa ccagtgcgcg
      541 tcgcgatcct tgagcagggt gaggtaatcg gagaatttct tgcgcttgat ctcgacgttg
      601 gcttcccggg agaagatcag gtagatgaag aacaccagga tggcggggat gagcgcgagg
      661 ccgaagacat tctgccagcc gaacgcctgc gccagccggg gggcgaagag cgcgtccagc
      721 acggtgccga tattgccggc tccggccagg cccatgacga gcccctgcat gttcggcgga
      781 taccagcggc ccgcctgcgg cagcgccacc gcgaaactcg cgccggcaaa gcccaggatg
      841 aagcccatta acagcgtggc ctggaagtcc cgcaggccca caagccaggc atagagcagg
      901 ccgacgatga cgatgatctg ggcgatgagg ccggttttct tcgcgccgat gtggtccacg
      961 gtcacgccga ggacgattcg caggatggcg ccagccagga tcggcatggc caccatcaag
     1021 cctttttcct gggcgttgag tcccagggat tccccgatct gggcgccgag cgggccgagc
     1081 agggtccaga ccatgaagct gacgtcgaaa tacaggaagg ccgtaatcaa cgtcggcaga
     1141 tggccggaat gtttgaggtc agacagtttc attttttggt tttggtggtt ggtggtggtg
     1201 tggatgtttg gaggtgatct tcgaacgcgt cgaggaaggc ccggcgctgg ggaggcgtca
     1261 ggttgaccga tggtgaatcc gccagggtgt cgagcagcca gacgccgcgg tccacggtgg
     1321 cgcggttggt gtcgttcttg aaaaaataat gcagcggccc ggcttccggc ttctggccca
     1381 cgcccttttc cagcgggccg aggagcgagt tggccagcac ttcgcgcgaa accgggaaaa
     1441 ggcgccggct gtccaggacg ccgactaatt cctcgcggcc ttggggcgtg tcgcagtatt
     1501 gggcggcggt gacgatcgcc tggcgcagcc gggcgtattc ctccgcgcgc tggtgccgca
     1561 gggagtggct ggcgagcagc accttctcgg gatgccgcgg cgcgagcgtc gcgctggtcg
     1621 cgacgatcca gccgtcgccc gcggcggtgg cggccgaatt ccacggctca ccgacgcaga
     1681 agccgtcgat ctgccccgcc ttcatgtgtt cgcccaccag cgcgggcggc aggaaaacga
     1741 tgcggatgtc gcggtcggga ttcaccccgg cgtcccgcag ccatttgcgg agcaggaagg
     1801 gatgggtcga aaacgcggag accacggcga aggcgggctt gcgcggggcc tcgctccgca
     1861 gcaattggcg gagggtcgag ccgtcgcgaa cgcccttttc ccagagccgc cgggaaaggg
     1921 tgatggcgtt gccctggagg ctcagcacca ggtccgtgct gacctcgcag gggggggaat
     1981 gcgtgccgca aaggatggag taaagcatgc cggccggggc gtgggccgcg tccagctcgc
     2041 cgtaaacgag tttttcccga atcgaccccc agccgagttc gcggttgagc tgcacctgca
     2101 ggccgctgtc ctgaaataac cccagcgcct ccgccatgag caggggagcc gcgtccgcca
     2161 gcggcacgta gccgagccgg agaatgcctt gggagggccg aatcggagcg accttccggg
     2221 tggttggttt gggagccgcg cttgtcatga cgcaacctca taccaccgcc cgccccgggg
     2281 ctcactggga aaaataatga acgtaatgat tgtttttcaa tgataagatg aatagcattc
     2341 atcaatggcg ctcgacctgg atatacggct tttgcggatg tttgtcacgc tggcgcgcgt
     2401 gggcagccac acccgcacgg gcgcggttct caacctgacc cagtcggcca tcagccacgg
     2461 catgaagcgc ctggaggagc agctgggctg cgccttgatt tacaagaagg gaaagtcggc
     2521 gcacctgacg ccggaggggc ggcattttct cgggcaggtg ctgcgcattc tcgacagcct
     2581 ggaccgggcg gccgaatccg tctccgggcg gggtgaatcg cggacgaagc tcagcgtggt
     2641 cttcagcgcc gccatggcgc acgccattct cgcgccggtg ctgcgggagt tccgcgagtc
     2701 gtatccgaat ctctcgctca atgtccggct ggaggactcg cccgcggcga ttcgcgaagt
     2761 ggaggagggc aaggcggacc tggccgtggt ggtggactcc aatctgccgg cgggcatcaa
     2821 gccgcatccg ctgttcaagg accagctcca gctcctgatt tcgccgctgc atccgtggaa
     2881 taaaaaagac aagctgaccg tccgcgacct gcgcgatgag cattttttgc tctatcaac
//
LOCUS       DATDAY010000197         3289 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_189029, whole genome shotgun sequence.
ACCESSION   DATDAY010000197 DATDAY010000000
VERSION     DATDAY010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 3289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3289)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3289
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_189029"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2102)
                     /locus_tag="VIS74_03890"
     CDS             complement(<1..2102)
                     /locus_tag="VIS74_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY5792414.1"
                     /translation="MAFTDLFIKRPVLATVVNLIILLAGWVAIQTLNIRQYPKSDITV
                     IKVTTAYVGANADLVRGFITTPLERVIASADGIDYMESASAQGLSTISVHLKLNFDAN
                     AALTQIQAKVAQVRNDLPPEAEAPIIEIETADSQTAGMYLSFYSKDLDQNQITDFLTR
                     VVQPKLASVSGVQKADILGARTFAMRIWLKPDKMAALNISPSDVSQALAANNYLSAIG
                     KTKGSMISVNLVANTDLKTPDEFKQLVVMQSGGAIVRLEDIADVSLGAESYDDDVRFS
                     GQTATFMGIWALPTANSLDVIKHVREALPGIQKLLPAGMKASIAYDSTVYIDNAIHEV
                     IKTLGETLLIVIAVIYLFLGSFRAIVIPIVAIPVSLVGAAFLMLMAGFTINLLTLLAI
                     VLAVGLVVDDAIVVVENVERHLHEGKSPFDAALIGARELVGPIISMTITLAAVYAPIG
                     IQGGLTGTLFREFAFTLAGAVIVSGIVALTLSPMMSSKLLIKGSTEKGFAGWINRRFD
                     RIRAGYTRLLTATLNHRPAVYAAWGFMSLLIFPFTMLSNKELAPTEDQGFIFGIVQSP
                     PNATLDQTMLFASRINEIFQSFPETDNTFQIINPTGGFAGLVTKPWSERQRSTMQIQG
                     EAYVKSTAVPGIRLIPMTPPALPGGGNFDVEFVICSTAEPDVIVGLANQLVQKAMESQ
                     IFMFADTDLKFDQPQTEVV"
     gene            complement(2134..>3289)
                     /locus_tag="VIS74_03895"
     CDS             complement(2134..>3289)
                     /locus_tag="VIS74_03895"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEY5792415.1"
                     /translation="MNAPTQRRSRLPLILTILACLGFAGALAFYKIHQFQQAAAAGAA
                     FAIPPEAVTSLVVQEEEWQPTLNAIGSIQAVNGVTVSADLPGIVTEILFESGQTVQAG
                     QILVKLDTRQEEAQFKAALAKRQLAALSLNRSKGLLTKQVSSQADFDAATAEFQQADA
                     LAQEYQAMIARKTIHAPFAGVLGIREVNVGQYLQSGDKVVPLQSLDPIYVNFSLPQQS
                     LGDLAVGRKVEVAADGLNAQTFEGEINAINSVVDEATRNVRVQATLKNPGDKLRPGMY
                     VKTQVNLPATEKSILIPASSISYAPYGNSVFVLEEMTHEQTKKTYRGARQQFVKIGAS
                     RGDQVTILSGLNPGEEIATSGVFKLRNGVEVSVDNQTQPSNNPRPTPNDT"
BASE COUNT          586 a          948 c         1102 g          653 t
ORIGIN      
        1 acgacctcgg tctgcggctg gtcgaacttc aggtccgtat cggcgaacat gaagatctgg
       61 ctttccatgg ctttttgcac gagctggttg gcgaggccca cgatgacatc gggctcggcg
      121 gtcgaacaaa tgacaaattc cacgtcgaaa tttccgccgc cgggaagcgc cgggggcgtc
      181 atgggaatca ggcggattcc cggaaccgca gtggatttga cgtaagcttc gccctgaatc
      241 tgcatggtgg agcgctggcg ctcgctccag ggcttggtga ccagccccgc aaagccgccg
      301 gtgggattga tgatctggaa cgtgttgtcg gtttccggga agctctggaa aatctcgttg
      361 atccgcgacg cgaacagcat ggtttggtcg agcgtggcgt tcggcgggga ttgcacgatg
      421 ccaaagatga agccctgatc ctcggtgggc gccagctcct tgttcgagag catcgtgaag
      481 gggaaaatca agagcgacat gaagccccac gccgcataaa ccgccggacg gtggttcagg
      541 gtcgcggtga gcaggcgggt gtagccggcg cgaatccggt cgaagcgccg gttgatccat
      601 ccggcgaacc ccttttccgt ggagcccttg atgagcaatt tcgagctcat catcggcgac
      661 agcgtcaacg ccacgatgcc ggaaacgatg accgcccccg ccagggtgaa ggcgaattcg
      721 cggaagaggg ttcccgtcag gccgccctgg atgccgatcg gcgcatacac cgcggcgagc
      781 gtgatcgtca tggaaataat cggcccgacc agctcgcgcg cgccgatcaa cgcggcgtcg
      841 aagggcgatt tcccctcgtg caggtggcgc tccacatttt ccacgacgac gatggcgtca
      901 tccaccacaa gcccgaccgc gagcacgatg gccagcagcg tgaggagatt gatggtgaat
      961 cccgccatca acataagaaa ggccgcgccg acgagcgaaa ccggaatggc cacgatcgga
     1021 atcacgatcg cgcggaagga gccgaggaac aaatagatga ccgcgatgac gatgagcagc
     1081 gtttcgccga gcgtcttgat gacttcgtgg atggcgttgt cgatgtaaac ggtcgagtca
     1141 taggcgatgc tcgccttcat gccggcgggc agcagtttct ggatgccggg cagggcttcg
     1201 cgcacgtgct tgatcacgtc cagcgagttg gcggtaggca gcgcccagat gcccatgaag
     1261 gtcgccgtct ggccgctgaa acgcacgtcg tcatcgtaac tttcggcgcc gaggctgaca
     1321 tcggcgatgt cttcgaggcg cacgatggcg ccgccgcttt gcatgacgac gagctgtttg
     1381 aactcgtccg gcgtcttcag gtcggtgttg gcgacgaggt tgacggagat catcgacccc
     1441 ttggtcttgc cgatggcgga aaggtaattg tttgccgcga gcgcctggct gacgtccgag
     1501 ggcgagatgt tcagcgcggc cattttgtcc ggcttcagcc agatgcgcat ggcgaaagtc
     1561 cgcgcgccga ggatgtcggc cttctgcacg ccgctgaccg aggcgagttt cggctggacc
     1621 acgcgggtga ggaagtcggt gatctggttc tggtcgagat ccttggaata aaagctcaga
     1681 tacatgcccg ccgtctggct gtcggcggtt tcgatttcaa tgatcggcgc ctccgcctcg
     1741 ggcggcaggt cgttgcggac ctgcgcgacc ttggcctgga tctgcgtcag cgcggcgttc
     1801 gcgtcgaagt tgagcttcag gtgcacgctg atcgtgctga gcccctgcgc gctggcggac
     1861 tccatgtaat caatgccatc cgcgctggcg atgacccgct ccagcggcgt ggtgatgaat
     1921 ccgcgcacca gatcggcatt cgcgccgaca tacgcggtcg tgaccttgat gaccgtaatg
     1981 tcgctcttgg gatactggcg gatgttgagg gtctgaatgg ccacccagcc cgcgaggagg
     2041 atgatcagat taaccaccgt cgccagaacc gggcgtttga tgaataaatc ggtgaaggcc
     2101 atgcggcgga ggtttcctgg ttctcccggg ggatcaggtg tcgttcgggg tggggcgggg
     2161 attgttggag ggctgggtct ggttgtccac ggacacttcc acgccgttcc ggagtttgaa
     2221 aacgccggag gtggcgattt cctcgccggg attcagcccg gaaagaatgg tgacctgatc
     2281 gccgcggctg gcgccgatct tgacgaattg ctgccgcgcg ccccggtagg tctttttcgt
     2341 ctgttcgtgg gtcatttcct ccagcacgaa caccgagttg ccgtaggggg cgtagctgat
     2401 ggaggaagcc gggatcagga tgctcttttc ggtcgccggc aggttgacct gcgttttcac
     2461 atacatgccg ggccgcagct tgtctccggg attcttgagc gtggcctgga cgcggacgtt
     2521 gcgcgtggcc tcatccacca cggagttaat ggcattgatt tcgccctcaa aggtttgggc
     2581 gttcaatccg tccgcggcga cttccacctt ccggccgacg gcgagatcgc cgaggctctg
     2641 ctgcggcagc gagaaattga cgtagatcgg atcgagggat tgcagcggga ccaccttgtc
     2701 gccgctctgg aggtattggc ccacgttgac ttcccggatt cccaggacgc ccgcgaaggg
     2761 cgcgtggatc gtcttgcgcg cgatcatggc ctggtattcc tgcgcaagcg catccgcctg
     2821 ttggaattcc gcggtggccg cgtcgaagtc cgcctgcgag gaaacctgct tcgtcaggag
     2881 ccccttcgag cggttcaggc tcagcgcggc gagctggcgt ttggccagcg ccgccttgaa
     2941 ctgggcttcc tcctggcggg tgtcgagttt gaccaggatc tggcccgcct ggacggtctg
     3001 gccggattca aagaggattt ccgtgacgat ccccggaagg tccgcgctca cggtcacgcc
     3061 attgaccgcc tggatgctgc cgatcgcgtt aagcgtcggc tgccattcct cctcctgcac
     3121 caccagcgag gtgaccgcct cgggcggaat ggcgaaagcc gcgccggcgg cggcggcctg
     3181 ctggaattga tgaatcttgt aaaatgccag cgcgccggcg aagcccaggc acgccaggat
     3241 cgtcagaatc aagggcaggc gcgagcgtcg ctgggtggga gcattcatg
//
LOCUS       DATDAY010000198         4267 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_852355, whole genome shotgun sequence.
ACCESSION   DATDAY010000198 DATDAY010000000
VERSION     DATDAY010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 4267)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4267)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4267
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_852355"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(119..2704)
                     /locus_tag="VIS74_03900"
     CDS             complement(119..2704)
                     /locus_tag="VIS74_03900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792416.1"
                     /translation="MIRVVPFLKTVTFLGFLAVAASNGFAAAPQVRVIADFEKDAGGF
                     SDGLVRDATQGKAGKASGKVETDLSQVTKSAWTTIQTPLKLPYAIEKLSFWVKTSDAK
                     LVMVRIIDATGQTFQVRMNLAPNGQWQQVVIDNFAAGQSFGGAKDKRVHWPARNLAFI
                     IDKNSLQGGKGTLWFDQIEAVVGSQRIVGKLDILTEKPGNVFLTGENVQVAVDTQGDA
                     VKWSVKDFWGKEIGSGTAPVTDQRAVIPLKVPNPGYYEVQLAATKGGAPLAEGITTLA
                     VLTPFDLAKVKNSPFGIMTHFAQGWDRDVIPLAALAGIKDIRDELYWANLEKTKGQFS
                     FPREFDAYMAAAKAANLIPLTVLSFGNKNYDDTPNSPSYGNAPYTDAGRAAYTRYCLE
                     VLKHYGSQIEAAEIWNEYNGTFGKGPVAEDKAKYYFEMLKTVYGPIKQARPDLTVVGV
                     SAVTAPIPYFEDLFKRGALKYMNALSFHPYRTQSTPEGLEGVLKKVNALVAKYNGGKT
                     IPLWITELGWYVKGINSYSDVIVTEVDQAKYLVRAITLSVANNVEKFYWYHLRDDRSF
                     PSSGVLRSPEDPKGRHTPKPSYAAYANLIRQLNAATFVKREPALNEGVYSMLFNRGGE
                     EIRVLWSLEPVTLTAKAGVPLTVVDLMGVSRTVEPSGGVVNLPLTDIPVYVFGSLSDL
                     PPVQKAIGELVADSEEEFSSVQGDDGWSYGIFIAPAPGAPYDPAAWQQLPKYRTTIWG
                     YEWIGDQPHLFLTPGGGHPGRDGGKPIWVVRRWISDVEGNIRFKGLVGHGTQGDGTTG
                     RIFVDGKEVWKQLVGGGQPNQSKFDFTLPIKKGAVIDIAVDPGPGTDTSFDGTTSNVT
                     LTLMP"
     gene            complement(2758..3795)
                     /locus_tag="VIS74_03905"
     CDS             complement(2758..3795)
                     /locus_tag="VIS74_03905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792417.1"
                     /translation="MILVAAPGLWAETVGIADFETDSGFDSGLVVDDTVAKEGQKSGR
                     MDVDFEATPPVRWKTAAKSFDIQNEIQALSFWIKTSTVRRLAVRIVDSTGQTFQSRPV
                     ISPFDEWQQVEITSFTSGPGFQSFGGAADGVVHWPAKSLALILEKGGIADGGTVGTLW
                     IDQVELTVEGEPRSTAAAGLEAKTAGAEREVLADFEQGTMGFTGTIAEDSAQAKSGNS
                     SARIDADFSKPTVSWVSTGKKLAIPDAIKLLRFWVKSEGIGSLAVRLTDSTGQSHMLR
                     PMIKPGADWQEVVIADFTKGRGHQSYGGAGDGQVHWPVTSLAFVVEKNPAAPVQATVW
                     LDDIEIFHGPE"
     gene            complement(3910..>4267)
                     /locus_tag="VIS74_03910"
     CDS             complement(3910..>4267)
                     /locus_tag="VIS74_03910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792418.1"
                     /translation="AAACIVPVSPLVALSPSTGMVLALASLIALAHAAWLGNISALVV
                     DLIPPRIMATTFGVIAGGSALGGIAMNSLVSWMVKDFSYTPCFFVMALMHPIAIWLLW
                     RYRRVAAEASPSPAIH"
BASE COUNT          818 a         1349 c         1285 g          815 t
ORIGIN      
        1 tggaatcacc gagaaacgcc accttctgcc cattctgaac ggccgcttcc ccgccccgca
       61 cggactggca aaacgccaga ccgcccaaaa tcaaacccag cgccgcgctt ctcacgattt
      121 acggcatcaa ggtcagcgtc acattcgacg tggtgccgtc gaaggaggtg tcggtgcccg
      181 ggccgggatc gacggcgatg tcgatcaccg cgcccttctt gattggcaga gtgaagtcga
      241 acttcgactg gttgggctgg ccgccgccga cgagctgttt ccagacttcc ttgccatcca
      301 cgaagatgcg gcccgtcgtg ccatcaccct gggtgccatg acccaccagg cccttgaagc
      361 ggatgttgcc ctccacgtcg ctgatccagc gccggacgac ccagatgggc ttcccgccgt
      421 cccggccggg atggccgccg ccgggcgtga ggaaaagatg cggctggtcg ccgatccact
      481 cgtatcccca gatggtggtg cggtatttcg gaagctgttg ccaggctgcg ggatcgtagg
      541 gcgcgcccgg cgcgggggcg atgaagatgc cgtagctcca accgtcatcc ccctgcacgc
      601 tcgagaattc ctcctcgctg tccgcgacga gttcgccgat cgctttctgc accggcggca
      661 ggtcgctcag cgagccgaag acataaaccg gaatatcggt gagcgggaga ttcacgacac
      721 cgccggaggg ttcaaccgtc cggctcacgc ccatgaggtc caccaccgtg agcggcacgc
      781 cggctttggc ggtgagcgtc accggctcga gggaccacag gacgcggatt tcctcgccac
      841 cgcgattaaa gagcatggaa taaacgcctt cgttcaaggc cggctcgcgc ttcacgaagg
      901 tggcggcatt caattgccgg atcagattgg cgtaggcggc ataggacggt ttgggggtgt
      961 ggcggccctt cggatcctcc ggggagcgca acacgccgga ggaggggaac gaacggtcgt
     1021 cccgcaggtg ataccaatag aatttctcca cgttgttggc gacgctgagc gtgatggcgc
     1081 gcacgaggta tttggcctga tcaacctccg tgacgatcac gtcgctgtag ctgttgatgc
     1141 ccttcacata ccagccgagc tcggtgatcc agaggggaat cgttttaccg ccattgtatt
     1201 tggcgacgag cgcgttgacc tttttcagga ctccctcgag tccctccggc gtgctttggg
     1261 tgcggtaggg atggaaggaa agcgcgttca tgtatttcag cgcgccgcgc ttgaagagat
     1321 cctcgaaata cggaatgggt gcggtgaccg ccgaaacacc gaccacggtg agatccggcc
     1381 gggcttgctt gatcggccca tagaccgtct tgagcatctc aaagtaatat ttcgccttgt
     1441 cctcggcgac gggacccttg ccgaaggtgc cgttgtattc gttccagatc tcggcggcct
     1501 cgatctgcga gccgtagtgc ttgagcactt ccaggcaata acgcgtgtag gcggcgcggc
     1561 ccgcgtccgt gtaaggcgcg ttgccgtagc tgggggagtt cggcgtgtcg tcgtagtttt
     1621 tgttgccgaa ggacaggacc gtcagcggga tgagattcgc cgctttcgcc gccgccatgt
     1681 aggcgtcaaa ctcccgggga aacgagaact ggcccttcgt cttttccagg ttcgcccagt
     1741 agagttcgtc gcggatgtcc ttgatgccgg cgagcgccgc gagcgggatc acgtcccggt
     1801 cccaaccctg cgcgaagtgg gtcatgatgc cgaagggcga gttcttcacc ttcgccagat
     1861 caaagggagt gaggacggcg agcgtcgtga ttccttccgc cagcggagcg ccgcccttgg
     1921 tggcggccag ctgcacttcg taatacccgg gattcggaac tttcagcgga atgaccgcgc
     1981 gttgatccgt gacaggcgcc gtgcccgagc cgatctcctt gccccaaaaa tccttcacgg
     2041 accatttcac cgcatccccc tgggtatcca ccgcgacctg gacgttttcg ccggtgagga
     2101 aaacgttgcc cggtttttcg gtgaggatgt cgagcttgcc gacgatgcgc tgggaaccca
     2161 caaccgcctc gatctggtcg aaccaaagcg tgcccttgcc gccctgcagt gagtttttat
     2221 caatgatgaa cgccaggttt ctggccggcc agtgcaccct cttgtccttc gcgccgccga
     2281 agctctgccc cgccgcaaag ttgtcgatca ccacctgctg ccattggccg ttcggcgcaa
     2341 ggttcatgcg cacctggaac gtctgcccgg tggcgtcaat gatgcgcacc atcaccagtt
     2401 tcgcgtcgga ggttttcacc cagaaactca gtttctcgat ggcataaggc agcttgagcg
     2461 gcgtctgaat cgtcgtccag gcgctcttgg tgacttgcga aagatcggtc tccacctttc
     2521 ccgaggcttt gccggccttg ccctgcgtgg cgtcccgcac gaggccgtcg ctgaacccgc
     2581 cggcgtcctt ttcgaagtcc gcaatgaccc gcacctgcgg ggccgcggcg aacccgttgc
     2641 tggccgcaac ggcgagaaag ccaaggaaag tgacggtttt aaggaatggg acaactcgga
     2701 tcataaggag tggggatagg ttcagaaagc aaagcagagc agaggaccgg agcgggctta
     2761 ttccggtcca tggaaaattt caatatcgtc gagccagacc gtcgcctgga cgggagccgc
     2821 cggatttttt tcgacgacga aggcgaggct ggtcaccggc cagtgcacct ggccatcacc
     2881 cgcgccgccg taactttgat ggccccgccc tttggtgaag tcggcgatca ccacctcctg
     2941 ccagtcggcg ccgggtttga tcatcggacg cagcatgtgc gactgcccgg tcgagtcggt
     3001 cagacggacg gcgagactgc cgattccctc ggacttcacc caaaaccgca ggagcttgat
     3061 ggcatcggga atggccaatt ttttcccggt gctgacccag gaaaccgtgg gtttggaaaa
     3121 atccgcgtcg atccgcgccg aactgtttcc cgatttggcc tgcgccgaat cttccgcgat
     3181 ggttcccgtg aaacccatgg tgccctgctc gaaatcggcc agcacctccc gttcggcgcc
     3241 cgcggtcttt gcctccaggc ccgccgcggc agtcgaacga ggctcgccct cgaccgtcag
     3301 ctcgacctga tcaatccaga gggttcccac cgtcccgcca tccgcgatcc cgcctttttc
     3361 caggatcaac gccaggcttt ttgccggcca atgcaccacg ccatccgcgg ctccgccaaa
     3421 cgactggaag cccgggccgc tggtgaacga agtgatttcc acctgctgcc attcgtcgaa
     3481 cgggctgatc acggggcgcg attgaaacgt ttgcccggtg ctgtccacga tccgcaccgc
     3541 cagccgccgg accgtggagg ttttgatcca aaacgacaaa gcctggatct cgttctgaat
     3601 atcgaacgat ttggcggccg tcttccagcg caccggcggc gtcgcttcaa aatcgacgtc
     3661 catccggccc gatttctgcc cctccttggc caccgtatcg tccaccacca agccgctgtc
     3721 gaagcctgaa tccgtttcaa aatcggcgat gccaaccgtc tccgcccaca gcccgggagc
     3781 ggcaaccaaa atcatgccgc acaaaaaaca tcgaggatac cgactcaata aggcaagggg
     3841 gggcataagg aagaattctg cgaggaaaac catttctgtc aaatcggaat gattgctttc
     3901 ttatatcgat caatggatcg ccggacttgg tgatgcctcc gccgccacgc ggcggtagcg
     3961 ccagagcaac cagatggcga tgggatgcat caacgccatc acgaagaagc agggcgtgta
     4021 ggaaaaatcc ttcaccatcc acgacaccag ggaattcatg gcgatgccgc ccagcgcgct
     4081 gccgccggcg ataacgccga aggtcgtggc catgatgcgc ggcgggatga ggtccaccac
     4141 cagcgcggaa atatttccca gccacgcggc gtgggccagg gcgatcaacg aagccagggc
     4201 cagcaccatc cccgtcgagg gcgaaagggc caccagcggc gaaaccggca cgatgcacgc
     4261 cgccgcc
//
LOCUS       DATDAY010000199         3459 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_2314116, whole genome shotgun sequence.
ACCESSION   DATDAY010000199 DATDAY010000000
VERSION     DATDAY010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 3459)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3459)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3459
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2314116"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(120..1631)
                     /locus_tag="VIS74_03915"
     CDS             complement(120..1631)
                     /locus_tag="VIS74_03915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY5792419.1"
                     /translation="MPRFALFPLLAALTLCAGCHTLRVRVENPRPAPLPRGVTETQRL
                     TELLETARTNPANEAAVIEIIRRLAARKFDSPDIRLVRTGKQVLDPTTCTDLIPARDV
                     RIGGLERRSTQDGIGVPCVAYFKKDSPALAGQPGIAPAGMAIPVTAVLRFDTPRPTLC
                     FYTRLQGDSAVIDGRRVALAGDFSAPIAVVLSHGKNRSIDVEAMLFSRRNYSQMGLYQ
                     FEPYDPKKIPVVFVHGLLSRPEAWVHALNGLMADPQIRARYQFWFYLYPTGLPVWASA
                     AGLRRELDRFQRECSAIAPSPKLKQMVLAGHSMGGLICSMMIREGGNQLWEQFSDVPA
                     SQLRLTPEAKAEFLRFIYFLPRKDVSRVIFVATPHRGSRLALRPLAGFVANLIQLPLN
                     ALTPYRSQLIRSLREDVRGIFVSPANSIRFLRANSPLLLSILKLPMDKDVPFHTILGD
                     RGRGNSPNSTDGVVPYWSSHLDGAVSEKVVPSGHGANENPQGVEEIRRILREN"
     gene            1895..2842
                     /locus_tag="VIS74_03920"
     CDS             1895..2842
                     /locus_tag="VIS74_03920"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY5792420.1"
                     /translation="MKNPIQSNSSGSDFRSGLLTFAIVAGSSILFCSKGVFAKLAFAA
                     GADAVTVLALRMAFALPFFLAIAAGASRGAARLDAATWLRLAALGFLGYYFSSLVNFT
                     GLQYVSIGLERIILYTYPSIVLVISAFFLRRRVRPVVWLACAVAWIGICAAFAGELRH
                     PQAADQAVWGAFLIFLSALSYAGFILLSGGTIARVGVMRFTGLAVGFSCLFMLAHYGL
                     THDPRALGGLPAVVYGYGGILAVFGTVLPALLLSIGLKRAGAANFSIISAIGPVATFF
                     LAWALLGESPNAGQLAGFALTLAGGLGISLLRTEKVSPD"
     gene            complement(2939..3409)
                     /locus_tag="VIS74_03925"
     CDS             complement(2939..3409)
                     /locus_tag="VIS74_03925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792421.1"
                     /translation="MNPAAEQNREIERRFLIPAPPANLDLHPSRLVEQGYLASGETTV
                     RLRRDGESAFVLTVKRGVFPNTEEREALLTAAQFETLWPLTAGRRIRKTRHAIPHGPH
                     TIELDFFHGIHEGLRIAEVEFSDTASCETFVPPDWFGQEITGRREYSNAALARE"
BASE COUNT          578 a         1008 c         1173 g          700 t
ORIGIN      
        1 caacttccgc ctctggatcc gccacgtttt ggcggcgctt tttcgccttt gcttcccagc
       61 cctcccttcc tccccgcaac ccttcctccc gaaacttgct ttcggttttt aaggaccgac
      121 taattctccc gcaggatccg ccggatttcc tcgacgcctt gcgggttttc gttggctccg
      181 tggccggagg gcacgacctt ttcggaaacc gcgccgtcga ggtgcgaact ccaatagggc
      241 acgactccat ccgtgctgtt cggggaattc ccgcgcccgc ggtcgccgag gatcgtgtgg
      301 aacggcacgt ctttgtccat cggcagcttg aggatggaca acagcagcgg cgaattggcg
      361 cgcaggaaac gaatgctgtt cgccggagag acgaagatgc cgcggacatc ctcgcgcagg
      421 gatcggatga gctgcgaacg atacggagtc agggcgttga gcgggagttg aatcaggttt
      481 gccacaaatc ccgccagcgg ccgcagggcc agccggctgc cgcggtgcgg cgtcgcgacg
      541 aagatgacgc ggctcacatc cttgcggggc agaaaataaa tgaagcgcag aaattccgcc
      601 ttggcctccg gagtcagccg gagctgggaa gccggcacgt cggaaaattg ctcccagagc
      661 tggttgccgc cctcgcggat catcatgctg cagatcaggc cgcccatgct gtggccggcc
      721 aggaccatct gcttcaactt cgggctggga gcgatggcgg aacactcgcg ctggaagcgg
      781 tccagttccc tgcgcagccc ggcggcggaa gcccagacgg gcaggccggt ggggtagagg
      841 tagaaccaga attgatagcg ggcgcggatt tggggatccg ccatcaggcc gttgagcgcg
      901 tggacccagg cttccggccg ggaaagcagg ccgtgcacga acacgaccgg gattttcttc
      961 ggatcatacg gctcgaattg ataaagcccc atctgcgagt aatttcgccg cgaaaagagc
     1021 atggcttcca catcgatcga acgatttttt ccgtgggaga gcaccacggc aatcggggcg
     1081 gaaaaatcgc cggccagcgc cacgcggcgg ccgtcgatta cggcgctgtc gccctgcagc
     1141 cgagtgtaaa agcaaagcgt gggccgcggg gtgtcgaagc ggaggaccgc ggtgaccggg
     1201 atcgccatgc cggcgggagc gatgcccggc tgcccggcga gggcggggga atccttcttg
     1261 aaatacgcca cgcagggcac gccgattccg tcctgcgtgc tgcgccgttc caagccgcca
     1321 atccggacgt cccgcgcggg gatcagatcg gtgcaggtgg tggggtcaag gacctgcttg
     1381 cccgtgcgga ccagccggat gtcgggcgaa tcaaatttcc gcgcggccag ccgccggatg
     1441 atctcgatga cggccgcctc attcgccgga ttcgtccggg cggtttccag caactccgtg
     1501 agccgctggg tttccgtcac gccgcggggc aacggggcgg gacggggatt ttccacgcgg
     1561 actcttaacg tgtgacagcc ggcgcaaagg gtcagtgcgg ccagcaacgg gaataaagca
     1621 aagcggggca tgcgagtggg aaatggatac ggcgccaaga attggcagaa atcccgctgg
     1681 gattccatgc ttctttccgg gcaggattgg cttgcgggtt cggggaattc acgccaattt
     1741 gaacgcgcat gatgcgtttc ggaaaacttc ttccctggct tggccgtcct ctcggactgg
     1801 cgcggaagga ttttgcccgg aatcaatagg caagcatctc cccccttctt ccaccgcccg
     1861 cccgagagcc ttctccggcg ggtttttcgt tttcatgaaa aatcccattc aatccaattc
     1921 cagcggctcc gatttccgaa gcggtttgct gacgtttgcc atcgtcgcgg gcagctcgat
     1981 ccttttttgt tcgaagggcg tgtttgccaa gctggccttt gcggcgggag ccgatgcggt
     2041 gacggtgctg gcgctgcgca tggcgtttgc gttgccgttt tttctcgcga tcgccgccgg
     2101 ggcctcgcgg ggagcggcgc ggctggatgc cgccacctgg ctgcggctgg cggcgctggg
     2161 tttcctgggg tattacttct cgtcgctggt gaatttcacc ggcctgcaat acgtgagcat
     2221 tggcctggag cggatcattc tttacacgta tccatccatc gttctggtca tctcggcctt
     2281 cttcctgcgc cgccgcgtgc ggccggtggt ctggctggct tgtgcagtgg cgtggatcgg
     2341 catttgcgcg gcgttcgcgg gggagttgcg ccatccccag gcggcggatc aagccgtctg
     2401 gggggcgttt ctgattttcc tgagcgcgct gagttatgcc gggttcatcc tcctgagcgg
     2461 cgggacgatt gcgcgggtgg gggtgatgcg gttcacgggc ctggcggtgg gtttttcctg
     2521 cctgttcatg ctcgcgcact acgggctgac gcacgacccg cgggcgctgg gagggctgcc
     2581 ggcggtcgtt tacggatacg gcggcatcct ggccgtgttt ggcacggtgc tgcccgcgct
     2641 gctgctgagc atcggtttaa agcgggcggg ggcggcgaat ttttccatca tttccgccat
     2701 cggcccggtc gcgaccttct ttctcgcctg ggcattgctg ggcgagtccc ccaacgcggg
     2761 ccagctggcg ggcttcgcgc tgacgctcgc gggcggcctg ggcatcagcc tgttgcggac
     2821 ggagaaggtt tccccggact gaacgaaacg ggattgcggg attggccgga ttcacgggat
     2881 ttaaagaaaa tcctgggatc cggtaaatcc ggtaaatccg gtttcatttg gaaaacgctt
     2941 attcccgggc cagcgcggcg ttggagtatt cgcgccggcc ggtgatttct tgcccgaacc
     3001 aatcgggcgg gacgaaggtt tcgcagctcg cggtatcgga aaactccacc tcggcgatgc
     3061 gcaggccctc gtggatgccg tgaaagaaat cgagttcgat ggtgtgcggt ccgtgcggga
     3121 tcgcgtggcg cgttttgcgg atgcggcgcc cggcggtcag cggccaaagg gtctcgaatt
     3181 gcgcggcggt cagcagcgct tcgcgctcct cggtattggg gaaaacgccg cgcttgacgg
     3241 tgagcacaaa ggcggattcg ccatcgcggc ggaggcgcac ggtggtttca ccgctggcga
     3301 gataaccttg ttccaccagc cgggagggat ggaggtcgag attcgcgggc ggggccggaa
     3361 tcaggaaacg tctttcgatt tcgcggttct gttcagcggc cggattcacg ggaatttttt
     3421 aaccacagag aacgcagaga gcacagagat aattgcgga
//
LOCUS       DATDAY010000200         4294 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_1171159, whole genome shotgun sequence.
ACCESSION   DATDAY010000200 DATDAY010000000
VERSION     DATDAY010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 4294)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4294)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4294
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1171159"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1190)
                     /locus_tag="VIS74_03930"
     CDS             complement(<1..1190)
                     /locus_tag="VIS74_03930"
                     /inference="COORDINATES: protein motif:HMM:NF018434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY5792422.1"
                     /translation="MRILLAPLGSEGDVNPFLWLAAGLKARGHAVTLLLSPYYGWLAD
                     ARGLAWQPVGIAEDFLKFAQTPLIWHPRRGTKLVIQGTLAQTESFRAAFEKTGGDFDL
                     LITSTLGFAISFLAEARGIPRLHVHLQPVCLRSVHDPPLFLHGMEWLKTSPLWLKRAF
                     AGFIDWKLNRLMRRAVNEFRRGLGLRPIHQVHAEVLMAGERLAGLFPGWFAQPQPDWP
                     GNFRQFGFPLPPVAPAPLAPELEAFLAAGPPPVLWSHGSANFHVEEFQAAALAASREL
                     GERCLLVSLEPPAGPLPEFAFHVPHARFEHLFPRCRAVVNHGGIGTISKAMAAGCPQL
                     AVSMAHDQPDNAARLRKLGIGAEVPRPKLTPQRAAAALRHLLHSPEVEARCAEIRDRL
                     RRANP"
     gene            complement(1192..1839)
                     /locus_tag="VIS74_03935"
     CDS             complement(1192..1839)
                     /locus_tag="VIS74_03935"
                     /inference="COORDINATES: protein motif:HMM:NF025380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4230 domain-containing protein"
                     /protein_id="HEY5792423.1"
                     /translation="MKLRTAIGISLILLSLAAAGYWLAVRAPSAAAREVARQLRAEFA
                     SAFQFQPEIRVESRTVLGPSSQILELATARREIWQRERREHEWFGSVKVFEVEGRFAA
                     RAGYDLQQPLTLDIREAPRQIRVTLPPAKLLGLELAEIKIRADADGWWNKLTGQDREQ
                     ALAGLQLAARRKIQEAGLLKEARENLEKSVRELLEKTAPGFAVSFDPATLPEGKQ"
     gene            1895..3349
                     /locus_tag="VIS74_03940"
     CDS             1895..3349
                     /locus_tag="VIS74_03940"
                     /inference="COORDINATES: protein motif:HMM:NF017669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA-like domain-containing protein"
                     /protein_id="HEY5792424.1"
                     /translation="MPIAFAGAPLGLNPKMANRHGLIAGATGTGKTVSLRVLAERFSD
                     LGVPVFVADIKGDLGGLSQPGTANPKIEERINAFGLRDFAFAGFPVCFWDVFGEKGHP
                     VRTTISEMGPLLLARLLSLNETQAGVLTLLFKYADDRGLLLLDLKDLRSLVQFAGDNA
                     KELRAEYGQVSPTSVGAIQRALLALETGGGERLFGEPALRLDDLLRVGAEGRGTIHLL
                     AADRLMQAPQTYATLLLWLLSELFEQLPEIGDPEKPRLVFFFDEAHLLFDELPKAIRD
                     KVEQVVRLIRSKGVGVYFVTQSPLDIPGEILGQLGNRIQHALRAFTPRDQKAVKAAVQ
                     TFRQKPGLDLESAILELKVGEAIVSLLDDSGSPAPAERALIYPPRSSLAPLAGAAREQ
                     LIRRSPLYGIYEKTVDRESACERLKKAAQPAVQPPPLPPERPAPRPKAAPKSRAQTPG
                     EAMVKSFLRSAGTALAGALVRGLLGSLKANMRRR"
     gene            complement(3394..3466)
                     /locus_tag="VIS74_03945"
     tRNA            complement(3394..3466)
                     /locus_tag="VIS74_03945"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3431..3433),aa:Ala,seq:cgc)
     gene            complement(3808..4119)
                     /locus_tag="VIS74_03950"
     CDS             complement(3808..4119)
                     /locus_tag="VIS74_03950"
                     /inference="COORDINATES: protein motif:HMM:NF025574.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS-like domain-containing protein"
                     /protein_id="HEY5792425.1"
                     /translation="MKLELQLTEDFGTLLSDGSRALEYRIARIDPYLTLCEEIVIDFT
                     GVRHANSSFVNALVAGVIEQHGEAALRLLVFKGCNPAIKVLIEAAIDLGLQKVADKMC
                     A"
BASE COUNT          815 a         1340 c         1385 g          754 t
ORIGIN      
        1 gggttggccc ggcgcaggcg gtcccggatt tccgcgcagc gcgcttccac ttcgggggaa
       61 tggagcaaat gccgcagcgc cgcggccgcg cgctgcgggg tcagtttcgg gcgcggcacc
      121 tccgcgccga tgcccagctt ccgcaaccgc gcggcattgt ccggctggtc gtgggccatc
      181 gaaacggcca gctgcgggca cccggcggcc atggccttgg aaatcgtgcc gatgccgccg
      241 tggttcacca ccgcccggca gcggggaaac aggtgctcga agcgggcgtg cggcacgtga
      301 aacgcgaact ccggcagcgg tccggccggc ggttcgaggc tgacgagcag gcagcgttcg
      361 ccgagttccc gggaggccgc cagggccgcg gcctgaaatt cctccacgtg aaaattggcg
      421 gaaccgtgcg accacaggac gggcggcggc cccgccgcca ggaaggcttc caactccggc
      481 gcgagcgggg cgggcgccac gggcggcagc ggaaagccaa actggcggaa attccccggc
      541 caatctggtt ggggctgggc aaaccagccg ggaaacaagc cggcgaggcg ctctcccgcc
      601 atcaggacct ccgcatgcac ctggtgaatg gggcgaagcc ccaggccgcg gcggaattca
      661 ttgaccgccc ggcgcatgag gcggttcagc ttccaatcga tgaatccagc gaaagcccgc
      721 ttcaaccaga gcgggctggt cttcagccat tccattccgt ggaggaaaag cggcgggtca
      781 tgaacgctgc gcaggcagac gggctgcaga tgcacgtgga ggcggggaat gccgcgcgcc
      841 tcggccagga acgagatcgc aaaacccagg gtggaggtga tgagcagatc gaagtccccg
      901 ccggtttttt caaacgccgc gcggaaggat tccgtctggg cgagcgttcc ctggatgacc
      961 agcttcgtgc cgcgccgtgg atgccagata agcggggttt gcgcgaactt cagaaaatcc
     1021 tccgcgatgc ccaccggctg ccaggcgagc ccgcgcgcgt cggcgagcca gccgtaatag
     1081 ggcgagagga gcagcgtgac ggcgtggccg cgcgccttca acccggcggc cagccagagg
     1141 aagggattca cgtcgccttc gctgccgagg ggtgccagca ggatgcgcat cttattgctt
     1201 gccttccggg agggtggcgg gatcaaagct gaccgcaaac cccggcgccg ttttttccag
     1261 aagctcccgc acggattttt ccagattctc ccgggcttcc ttaagcaggc cggcttcctg
     1321 gatttttctc cgcgcggcga gctgcaggcc ggccagggcc tgctcgcggt cctggccggt
     1381 cagtttattc caccagccat ccgcatcggc ccggatcttg atttcggcca gctccagccc
     1441 cagcaatttg gctggcggaa gcgtgacgcg gatctgccgg ggagcctcgc gaatgtcgag
     1501 ggtcaggggc tgctgcagat cgtaaccggc gcgggcggcg aagcgcccct cgacctcgaa
     1561 gaccttcacg ctgccgaacc attcgtgctc ccgccgctcc cgctgccaga tttcccgccg
     1621 ggccgttgcc agctcgagga tctgggaact cgggcccagg accgtgcggg actcgacgcg
     1681 gatttccggc tggaattgga aggcgctcgc gaactccgcg cgaagctggc gggccacctc
     1741 ccgcgccgcc gccgagggcg cccggaccgc cagccaatac ccggcggccg cgagcgagag
     1801 aaggatcagg gagatgccga tggccgtccg gagtttcatt tgtggctggt cgctccccgc
     1861 actttgcggc ataaatgccg gcatgcaaga cctcatgccg attgccttcg ccggcgctcc
     1921 cctgggcttg aatccgaaga tggccaatcg ccacggtctc atcgcgggag ccaccggcac
     1981 gggcaaaacg gtttcgctgc gcgtgctggc ggagcggttc agcgatctcg gcgtcccggt
     2041 gttcgtggcg gatatcaagg gcgacctcgg cggcctgagc cagccgggaa cggcgaatcc
     2101 gaaaattgag gaacgcatta acgccttcgg cctccgcgat ttcgccttcg cggggtttcc
     2161 cgtctgcttt tgggatgtgt ttggcgagaa ggggcacccg gttcggacga cgatcagcga
     2221 gatggggccg ctcctgctcg cgcggctgct cagcctcaat gaaacccagg cgggcgtgct
     2281 gacgctgcta ttcaaatacg ccgacgaccg cggcctgctg ctgctggatt tgaaggactt
     2341 gcgctcgctg gtgcaattcg cgggggacaa tgcgaaggag cttcgcgcgg aatacggcca
     2401 ggtttccccg acgagcgtgg gcgccatcca gcgggcgctg ctcgcgctgg aaacgggcgg
     2461 gggcgaacgc cttttcggcg agccggccct gcggctcgac gacctgctgc gcgtcggggc
     2521 ggagggccgc ggaaccattc acctcctcgc cgccgaccga ttgatgcaag ccccgcagac
     2581 ctacgcgacg ctcttgttgt ggcttttgtc cgaattgttc gagcagcttc ccgagatcgg
     2641 cgatccggaa aaaccgcggc tcgttttttt cttcgacgag gcgcatctgc tcttcgacga
     2701 attgcccaag gccatccgcg acaaggtgga gcaggtggtg cggctgatcc gctcgaaggg
     2761 cgtcggcgtg tatttcgtga cgcagagccc gctggacatt cccggcgaaa tcctcggcca
     2821 gctcggcaat cgcatccagc acgcactgcg cgccttcacc ccgcgcgatc aaaaggcggt
     2881 caaggccgcc gtccagacgt tccggcaaaa gccgggactc gatctcgaaa gcgcgatcct
     2941 tgagctcaag gtcggcgagg ccatcgtgtc gctgctggac gactccggtt cgccggctcc
     3001 cgcggaacgc gcgctgattt atccgccgcg cagttcgctg gcgccgctgg ccggcgcggc
     3061 gcgcgagcaa ctcatccgcc gctcgccgct ctacggcatc tacgaaaaaa ccgtggaccg
     3121 cgaatccgcc tgcgagcgcc tcaaaaaagc ggcccagccc gcggttcagc cgccgccgct
     3181 tccgccggag cggccggcgc cccggccgaa agccgctccg aaaagccgcg cccagactcc
     3241 cggagaggcg atggtgaaaa gcttcctgcg cagcgcgggc acggcgcttg cgggagcgct
     3301 ggtgcgcggg ctcctgggat cgctgaaagc caatatgcgc cggcggtgag cgccggatgc
     3361 catcgaatct ccgcggccgc gacagcgaaa ccgtggaggt atggggattc gaaccccaga
     3421 ccttctcatt gcgaacgaga cgctctacca actgagctat acccccttcg atggaactga
     3481 ggacgatagc agaactcccg gggatgggaa acggaatttt gcggatctag attttgccgc
     3541 aaaggaacgc aaaaaataac ctttcgcgtt ctttgtggcg atgacctaac tcttcggagg
     3601 tcggcatgtt ttcattttcc gctccacctc tctccgggac tgaacatcta gccaagctcg
     3661 aggccatcaa gcaagtcaaa gcctggggta aggcggttga gaaagccaca cccggattgg
     3721 agcaggatgc gggccaagag aaataagcta cacggtcgag ggagttaccg tgtgtaactt
     3781 tcggtttggg ctccgatgga gcccaaacta agcgcacatt ttgtcagcaa ccttttgaag
     3841 gccaagatca attgccgctt ctatcaaaac tttaattgca ggattgcacc ctttgaagac
     3901 aagcaaacgc aaagctgcct cgccgtgctg ttcaatgaca ccagcgacca atgcgtttac
     3961 aaacgagctg tttgcatggc gcacgccagt aaaatcgata acgatctctt cacaaagagt
     4021 gaggtaggga tcaatccgcg cgatgcggta ttccaaagct cttgatccat ccgagagaag
     4081 cgttccgaaa tcttctgtca attgaagttc gagtttcacg gtttaaaaat agctgatact
     4141 ctgccggatt gcaacagtcc agcatcaatt ttggctgcat ggaggatgtc ggcaaacgct
     4201 ctaacgctat cttggcgaaa ggtaaggata agcaatgttc cgggatacga tccttgattt
     4261 gggagttctg aaaaactcat ttcacggttt ggaa
//
LOCUS       DATDAY010000201         4103 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_1773956, whole genome shotgun sequence.
ACCESSION   DATDAY010000201 DATDAY010000000
VERSION     DATDAY010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 4103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4103
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1773956"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1034
                     /locus_tag="VIS74_03955"
     CDS             <1..1034
                     /locus_tag="VIS74_03955"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HEY5792426.1"
                     /translation="NWWGGEKSLYRKKNGYLYLPQFAMLYTPYELLPDRVGEPLWRLT
                     CLGSLALALWVAVSHLSRENRNTLFLLATVLVLPASFSSARNGQVNMPLAALFLLTAI
                     ALSRERWWTSAILLAVTLILKPIALAPILLCAALYPKLRLPMLACLAVAFLLPFAHFN
                     PAYAAGEYGNFVKNLGQAGAPRGQTWCDFAGMLHKFGLELPSAVQLAARALAGVLALG
                     LGWKALRALDPLRGALVVLLLSMVYLMLFNPRTETNSYIMLAAFGAVFGAYSGLIGKR
                     LGEAAGFALLAAILGTENYGNPIFPWTNLWLKALATCVLAAWLAKKILSGPASARTLL
                     APAPTRAFQ"
     gene            complement(1193..2431)
                     /locus_tag="VIS74_03960"
     CDS             complement(1193..2431)
                     /locus_tag="VIS74_03960"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HEY5792427.1"
                     /translation="MSNLSTNRKVLGFIDAWGGGAACAALTLLRRCWPFGGPDEAGRK
                     GLLFLKLAEQGSTVLASQAIRDAAERHGAENLYFLVFEENRFILDALEIIPPENVLAI
                     RTKGAASMALSGLGQLFRIRRLRLLACVDLEFFARSTAVIGFLTGIPRRVGFHGWHSE
                     GPFRGNLFTHRLRYNPHVHTSVIFRSLVAAVDADAQALPRFDWVAAEEPITPPAFRAT
                     EAEIAKIRGLIKQAAGGTPGSLVLLNANASDLLPLRKWANENYVELARRILDEVPDAW
                     IAFTGGPGEREKIDALAATVGRERVFSMAGRTSLRELLALYAQSDVLVTNDSGPAHFA
                     ALTPVDTVVLFGPETPKLFATLSARNQSLTANLACSPCVSALNNRQTDCTNNVCMQRI
                     TVAQVFAAVRNALEHRAKVN"
     gene            2576..3979
                     /locus_tag="VIS74_03965"
     CDS             2576..3979
                     /locus_tag="VIS74_03965"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY5792428.1"
                     /translation="MRGLPSARIVAHSRAVQEPPPTPHDAYDALRYPGCRDYLIGGFA
                     ANVGRQMATVAVVWQIYQWTHSATALGLVGLINVLPLLVLSLPAGMMADRLDRKWIIA
                     FSSAGMALLSLLLALVTQFSAAIPPLPILAGLNETLAVLARSLEQHPGARAMTFDQPA
                     LPIIYGILFLMAILRILGWPSRSAIIPLLVPAKSLSNAVTWNSSAFEISTMLGPAIGG
                     FLLAWAGYPAVYVLDAICGLSLALLIGRVRYETPPASASADSRSLRGIWAGVEFIWNK
                     KAILAASSLDLFAVLLGGAVALLPIYAEEILHAGPIGLGWLRAAPSIGAFSMALWLAH
                     RRPLQQPGVTLLWVVAGFGLSIIVFGFSPWFWLSLLALFFTGVFDNISVVVRQSLVQL
                     LTPDSLRGRVTAVNQIFIGSSNEIGTLRAGLMAAVTGPVAAVVIGGIGTIAVVIGVAR
                     FLPQLRATPALDKLKAD"
BASE COUNT          643 a         1372 c         1292 g          796 t
ORIGIN      
        1 agaactggtg gggcggcgaa aaaagcctgt atcgcaagaa aaatggctac ctctacctgc
       61 cgcagttcgc gatgctctac acgccctacg agctgctgcc cgaccgcgtc ggcgagccgc
      121 tctggcgcct gacctgcctc ggttcgctgg cgctggcgct ctgggtcgcg gtatcccatc
      181 tctcgcggga aaaccgcaat acgctcttcc tgctcgcgac cgtcctggtc ctgcccgcgt
      241 ccttttccag cgcgcggaat ggccaggtga acatgccgct ggccgccttg tttctgctga
      301 cggccatcgc gctttcgcgg gagcgatggt ggacctccgc cattctgctc gccgtcacgc
      361 tcattctcaa acccatcgcc ctggctccca tcctgctctg cgcggcgctg tatcccaagc
      421 tgcgcctgcc gatgttggcc tgcctggcgg tggccttcct gctgccgttc gcgcatttca
      481 accccgccta cgccgccggg gaatacggga atttcgtcaa aaatctcggc caggccggcg
      541 cgccgagggg ccagacctgg tgcgacttcg cgggcatgct gcacaagttc ggattggaat
      601 tgccgtccgc cgttcagctc gccgcccgcg ccctggccgg ggtgcttgcc ctcgggcttg
      661 gctggaaagc cctgcgggcg ctcgatccgc tccgcggcgc gctggtcgtg ctgttgctct
      721 ccatggttta tctcatgctc tttaatcccc gcaccgagac gaattcctac atcatgctcg
      781 ccgccttcgg cgcggtcttc ggggcgtatt ccgggctcat cgggaagcgg ctcggcgagg
      841 ccgccgggtt cgcgctgctc gcggcgatcc tcggcacgga aaattacggc aatcccattt
      901 tcccgtggac caatctctgg ctcaaggcgc tcgccacctg cgtgctggcc gcgtggctgg
      961 cgaagaaaat cctcagcggc ccggcttcgg cgcggacgct gctggctccc gctcccaccc
     1021 gggctttcca ataattcctt gctggagggc ggcgggccct cgccgctgtg attggatttg
     1081 gcgaagggaa aagcaacgga cggctcatcg agccgtccct gccattaatg ggcagggcgc
     1141 tgtcgccgac agcgccgtga atttttacca aaccgactct ctatatccgg tgttaattca
     1201 ctttggcgcg atgctccagc gcattcctga ccgcggcgaa gacctgcgcg accgtgatgc
     1261 gctgcatgca cacattgttc gtgcagtcgg tctggcggtt gttcagggcg ctcacgcacg
     1321 ggctgcacgc gagattggcg gtgagggatt gattccgcgc cgagagggtg gcgaataatt
     1381 tcggcgtctc cgggccgaag agcaccacgg tatcgaccgg cgtcagcgcc gcgaagtgcg
     1441 cggggccgct gtcgttggtc acgagcacgt cggactgcgc gtaaagcgcc agcagctcgc
     1501 gcaggctcgt ccgtccggcc atggaaaaga cgcgttcccg tccgaccgtg gccgccagcg
     1561 cgtcaatttt ttcacgctcg cccggcccgc ccgtgaaggc gatccaggca tccggcacct
     1621 cgtcgagaat ccgccgcgcc agctcgacgt aattttcatt cgcccacttg cgcagcggca
     1681 gcagatcgct ggcattggcg ttgagcagga cgagggagcc cggcgttccg cccgcggctt
     1741 gcttgatcaa cccgcggatc ttggcgatct ccgcctcggt cgcgcgaaag gccggcggag
     1801 tgatcggttc ctccgccgcg acccagtcga agcgcggcaa ggcctgggca tccgcatcca
     1861 ccgcggcgac gagcgagcgg aaaatgacgc tcgtgtggac gtgcggattg taacgcaggc
     1921 gatgggtgaa gagattgccg cgaaacggcc cctccgagtg ccagccgtga aaacccacgc
     1981 gccgggggat gccggtcaaa aatccgatga ccgccgtcga acgcgcgaaa aattccagat
     2041 ccacgcacgc cagcaatcgc agccggcgga tgcggaaaag ctggcccagg ccggaaagcg
     2101 ccatgcttgc cgcgcctttc gtgcgaatgg ccagcacgtt ttccggcgga atgatttcca
     2161 gcgcgtcgag gatgaaccgg ttttcctcga agaccagaaa gtaaagattc tccgcgccgt
     2221 ggcgctccgc cgcgtcgcgg atcgcctggc tggccaggac ggtcgagccc tgctccgcga
     2281 gcttgaggaa cagcagcccc ttgcgcccgg cttcgtccgg gccgccaaac ggccagcatc
     2341 gccgcaggag cgtcagggcc gcgcacgccg cgccgccgcc ccaggcgtca atgaacccga
     2401 ggacttttcg attggtggac agattgctca agatgacacg gagagaattc cgtccagtgt
     2461 agaggcgatg aaataaatgg gcaaggtcgc gttgagaggg tggttcgatt gaaattaacg
     2521 caaaatggag ggcgccgccc tccaagggca aaatcccgcc aaagcgaaag ggggaatgcg
     2581 gggattgccg agcgcccgga tcgtggcgca tagtcgcgcg gtgcaggaac cgccgcccac
     2641 ccctcatgac gcctacgacg ccttgcggta tccgggctgc cgggattatc tcatcggcgg
     2701 attcgccgcg aatgtcggcc gccagatggc caccgtggcg gtggtctggc agatttacca
     2761 atggacgcac tccgccaccg cccttggcct ggtggggctc atcaacgtcc ttccgctgct
     2821 cgttttgtcc cttcccgccg gcatgatggc cgaccggctg gaccgcaaat ggatcatcgc
     2881 gttcagctcc gccggcatgg cccttctttc cctgctgctc gcgctcgtga cgcagttcag
     2941 cgccgccatt ccgccgctgc cgatcctggc gggcctgaat gaaactctgg ccgtcctcgc
     3001 ccgctcgctt gaacagcatc cgggggcgcg agcgatgacc ttcgaccagc ccgcgctgcc
     3061 gatcatctac ggcattctgt ttttgatggc gatcctccgc atcctcggct ggccgtcgcg
     3121 ctcggccatc attccgctcc tcgtgccggc gaaatccctg agcaacgccg tgacatggaa
     3181 ttccagcgcg tttgaaattt cgacgatgct gggtccggcc atcggcggct ttctcctggc
     3241 ctgggcgggg tatcccgccg tttacgtgct cgacgcgatt tgcggactgt ctctggcgct
     3301 gttgattggc cgggtgcgtt acgaaacccc gcccgcttcg gcctcggcgg acagccgctc
     3361 gctgcgcggt atttgggcgg gcgtggaatt catctggaac aaaaaggcga tcctcgccgc
     3421 gagcagcctg gatttgttcg ccgtgctgct cggcggcgcg gtggccctgc tgccgattta
     3481 tgcggaggaa attctccacg ccggccccat cggccttggc tggctgcgcg ccgcgccgag
     3541 catcggcgcg ttttccatgg cgctctggct cgcgcaccgc cgcccgttgc aacaacccgg
     3601 ggtgaccctg ctctgggtgg tggccggttt cgggctgtcg atcattgtct tcggtttctc
     3661 cccgtggttc tggctctcgc tcctcgcgct gtttttcacc ggcgtgttcg acaacatcag
     3721 cgtcgtcgtg cgccagtcgc tggtgcagct gctcacgccg gattccctgc gcgggcgcgt
     3781 caccgcggtg aaccagatct tcatcggctc ctcaaacgaa atcggcaccc tgcgcgcggg
     3841 attgatggcg gccgtcaccg gccccgtggc cgcggtggtg atcggcggta tcggcaccat
     3901 cgccgttgtc attggagtcg cccgcttcct tccgcaactc cgcgccacgc ccgcgttgga
     3961 caagctcaag gcggattgat cgttcgtgag gcttatttcc aaaccggcgg tccatagccg
     4021 atcagcggct tgtcggaacc cgccttgggg atccgaaaat ccacgatgga aaagccaaac
     4081 tgccgctggc cgcggtagga ccg
//
LOCUS       DATDAY010000202         2786 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_2545123, whole genome shotgun sequence.
ACCESSION   DATDAY010000202 DATDAY010000000
VERSION     DATDAY010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2786)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2786)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2786
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2545123"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            93..2633
                     /locus_tag="VIS74_03970"
     CDS             93..2633
                     /locus_tag="VIS74_03970"
                     /inference="COORDINATES: protein motif:HMM:NF042912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Amuc_1098 family type IV pilus outer membrane
                     protein"
                     /protein_id="HEY5792429.1"
                     /translation="MIPRFLPIILAASLTGQFLPLSLQAQQPGVAGTAEREIHRRELS
                     AQYAQAQINKGAEALMAQDYESAFSYYKSAVDSLPSGGASTETVRENALDGFSRAAVS
                     LAKQRISEGRYQDAEMIVGVVLDERYNPNYAPALELAQQLRDPTHFNRTITPGFVANV
                     EEVKRLLGEAAGYYQSGRYDLAFRRYEQVLNIDKYNIAARRGMEQVNQQRKLYQETAY
                     NQTRSELLKDIDEGWELPVRKYNLGISSIIEQPQIDLSGRSNINRKLDEIIIPKVEFR
                     DATVREALDFIKQRAAALDTSEPKGVNIVLKLDPAAEAAESMSRITLQLTQVPLRAAL
                     EYIATAAGLKVKVEPYAVAIVPLSESTEVLLTKEYNVPPSFINTLPGDSSGGGTAPAA
                     PGGVTTRQGVQQYLEIQGVTFPTGSSAQYLPSTSKLIVRNTQNNLDLIDNLIEISLST
                     PPTQIEIQAKFLEVSQNNLKELGFDWLLGQFAFPFGTGVYSSGGTTGNQASNNSFYPI
                     TNPSGIPIGMSNGSTSTTAGSTTINGTGQLTAGNRSGGTAVRANALDGLLFGSPAGPA
                     AGILSLAGIFTNPQFQVVLRALDQQKGVDLMSAPSVVTQSGKQASIEIVRRFRYPTEY
                     DPPQVPQTQASGAVQPVTPATPSSFETRNLGVTLQVEPTVGPDGYTIDLHLHPQVSEF
                     EGFVNYGSPINTLAPVVRNSLIVAGSGTDVVISLGQIPIATRPVTLTENVINQPIFSI
                     REVDTNVTIYDGQTIVLGGLMREDVQKTEDKTPIIGDIPLVGRLFRTSADQRIKRNLI
                     IFVTANLIDPAGQPLIKSVEDDQEVPAPSAAESLNEIIPGDAASISTP"
BASE COUNT          625 a          916 c          701 g          544 t
ORIGIN      
        1 tctgaatgca gccaaggatt gacacttgcg gaaatcgcat gcgaccattc ttatacctta
       61 ccccctaaaa ctccccgagc cgctttaacc acatgattcc acggttcctt ccgatcatcc
      121 tggccgcttc ccttaccggg caattcctac ccctctccct gcaagcgcag caacccggcg
      181 ttgccggcac cgccgaacgg gagatccacc ggcgcgaact ttccgcccaa tatgctcagg
      241 cgcagattaa taaaggagcc gaagccctga tggcccagga ttacgaaagc gcgttttcct
      301 attacaaaag cgccgtggat tccctcccca gcggcggcgc ctctaccgag acggttcgcg
      361 aaaatgccct cgatggcttc agccgcgcgg ccgtcagcct tgccaagcag cgcatttccg
      421 aggggcgcta ccaggacgcg gaaatgatcg tcggggtggt cctcgatgag cgttataacc
      481 cgaattacgc gcccgcgctc gaactcgccc agcagttgcg cgatcccacc cactttaacc
      541 gaacaatcac cccgggattt gtcgccaacg tcgaggaggt caaaagactg ctcggggaag
      601 cggcgggata ttaccagtcc ggccgttatg atctcgcctt tcgccgttat gaacaggtgc
      661 tgaatattga caaatataac atcgccgccc gccgcggcat ggagcaggtc aatcagcagc
      721 gaaagcttta tcaggaaacc gcttacaatc aaacccgttc cgaattgctc aaggacatag
      781 acgaaggctg ggaactgccc gtccggaaat acaatctcgg gatttcctca atcatcgaac
      841 aaccgcagat cgatctaagc ggccgctcca acatcaatcg caaactggat gaaatcatca
      901 ttcccaaggt tgaattccgg gatgcgaccg tccgcgaggc tctggatttc atcaaacagc
      961 gggcggcggc tctggatact tcggagccga agggcgtcaa catcgtgctt aagctcgatc
     1021 cggctgcgga agccgccgag tccatgtcac gcatcaccct gcagctcacg caggttcccc
     1081 tgcgggcggc cctggaatac atcgccaccg ccgccggact caaggtcaag gtggaacctt
     1141 acgccgtcgc catcgttccg ctctccgaat ccaccgaagt cctgctgacc aaggaataca
     1201 atgttccgcc ctccttcatt aatactttgc ccggggacag cagcggtggc ggcactgccc
     1261 cggcggcgcc gggcggagtc accacccgcc agggcgtgca gcaatacctg gagattcagg
     1321 gagtgacctt ccccaccggt tcgagcgcgc aatacctgcc gagcacgagc aagctgatcg
     1381 tccggaatac ccaaaataat ttggatctga tcgacaatct gatcgaaatc tccctttcga
     1441 ctccccccac tcagatcgaa atccaggcca agttcctgga ggtatcccaa aacaacctga
     1501 aggaactcgg tttcgattgg ctgctgggac aatttgcctt cccatttgga accggggttt
     1561 atagcagcgg cggaactacc ggcaaccagg catcgaataa ctctttttat cccatcacca
     1621 atccctccgg catcccgatt ggcatgagca atggcagcac ctcaaccacg gctggcagca
     1681 ccacaatcaa cggaaccgga cagcttaccg cgggcaaccg ctccggcggc acggccgtgc
     1741 gggccaacgc gctggatggc ctgctcttcg gcagcccggc cggaccggct gcagggatct
     1801 tgtccctggc cggcatcttt accaaccccc aattccaagt ggttctgcgg gcgctggatc
     1861 aacaaaaggg cgtggatctg atgtccgcgc cttccgtggt gacccaaagc ggcaaacagg
     1921 cttcgatcga gatcgttcgc cgcttccgtt accccacgga atatgatcct ccccaggttc
     1981 cccagactca ggccagcggc gccgttcaac cggtaacgcc cgccactcct tcgagttttg
     2041 aaacccgcaa cttgggcgtg accctgcagg tcgaacccac cgttggcccc gacggctaca
     2101 ccattgacct gcacctccac cctcaggttt ccgagtttga gggttttgtg aactatggca
     2161 gccccatcaa cactctggct cccgtggttc gcaactccct aatcgtagcc ggcagcggca
     2221 ccgatgtagt aatttcgttg gggcaaatcc ccattgccac ccgccccgtc accctgaccg
     2281 aaaacgtcat taatcagccg atcttcagca ttcgggaagt ggataccaac gtcacgattt
     2341 acgatggcca gacgattgtg ctcggcgggt tgatgcgaga agacgtccag aagaccgaag
     2401 ataagacgcc gatcatcgga gatatcccct tggtaggtcg gcttttccga accagcgcgg
     2461 atcaacgcat caaacgcaac ctcatcatct tcgtcaccgc gaatctgatt gatcccgccg
     2521 gacaaccctt gatcaagtcc gtcgaggacg atcaagaagt tccggcgccc agcgccgcgg
     2581 agtccctcaa cgaaattatc cccggcgacg ccgcctccat ttccaccccc tagctaccgt
     2641 ccttaaatca tgacacttaa atcccagaca aggtcgttcc tttactgcgc catcctggcg
     2701 gtttcgaccg cttttcaagg ccacgcggga acccagggct ccggtagttc atctattgaa
     2761 agcattgccg accgcgaaat cgcccg
//
LOCUS       DATDAY010000203         2731 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_59077, whole genome shotgun sequence.
ACCESSION   DATDAY010000203 DATDAY010000000
VERSION     DATDAY010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2731)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2731)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2731
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_59077"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..638
                     /locus_tag="VIS74_03975"
     CDS             <1..638
                     /locus_tag="VIS74_03975"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY5792430.1"
                     /translation="IMSEPARVLRILIADDHVTVREGLSSILGRRPDMRVVAQASDGR
                     EAAGLWRQHRPDITLLDLRMPGLDGVALITELRREDPAARFIVLTTYDSDRDVSRAVQ
                     AGARGYLLKDAPTEELLACIHEVCSGGTAIHSALVSKLAAGLRSDPLTPRELDVLQLL
                     AEGKSNREIASGLFVGEATVKSHLHRVFAKLDVLSRTEAVAVATRQGLIRL"
     gene            701..1357
                     /locus_tag="VIS74_03980"
     CDS             701..1357
                     /locus_tag="VIS74_03980"
                     /inference="COORDINATES: protein motif:HMM:NF025875.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HEY5792431.1"
                     /translation="MPQLFPSSANTVTYAVFGTVLLGTAALIWAAVEITLSPYFTRVN
                     VPIGQPVPFSHMHHVEGLGIDCRYCHTSVEVASSAGIPSTETCMTCHSQVWKDAPVLQ
                     PVRDSFMTGKALEWRRVHNLPGFVYFDHSIHVKKGVSCMDCHGRIDEMPLVSKQHTLF
                     MKWCLDCHRHPESALVPPAAVFQMPAPPPREARSISRWMAEQNPRTRTIITDCTACHR
                     "
     gene            1354..>2731
                     /locus_tag="VIS74_03985"
     CDS             1354..>2731
                     /locus_tag="VIS74_03985"
                     /inference="COORDINATES: protein motif:HMM:TIGR04519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAT-variant-translocated molybdopterin
                     oxidoreductase"
                     /protein_id="HEY5792432.1"
                     /translation="MKTEPPPRAAWMGRFADQFPQWRSLEQLADTPEFREFLEREFPE
                     EASEWPDAMSRRRFLGLMGASLALAGLSACSRQPIEKLVPYVRQPPELTPGKPLYFAS
                     ADLLSGVARGIVVESHEGRPTKIEGNPRHPGSLGGTDALMQASILRLYDPDRSKTPLR
                     ERNVSTWDAFLAESQSLRNQWAKTQGAGLRFLSGQTTSPALLDQIQRFLTQFPAARWT
                     LHEPAVPAHPLQAVADLDRADVILAIDADFLVCGPGQLAYSRQFARRRDPEAGMNRLY
                     VAESFPSLTGAAADHRFSLKPADLARLPGWIEDALSGAPDPDAPSWVAAVVQDLERHA
                     GRGLVLAGDCLSGTAQEAVYRLNDRLGNIGQTLRYQSKWPEPAHPLPLRELVKEMAAG
                     RVETLFILDANPVYTCPADVPFAEAMAKVPLTVHHGLYVDETAARCVWHLPETDSLEA
                     WSDAAAFDG"
BASE COUNT          443 a          961 c          851 g          476 t
ORIGIN      
        1 gcatcatgag tgagcccgct cgcgtcctgc gcatcctcat cgccgacgac cacgtcaccg
       61 tgcgcgaggg gctttcctcc atcctcggcc ggcggccgga catgcgggtc gtcgcgcagg
      121 cctccgatgg gcgcgaggcc gcggggctct ggcggcagca ccgccccgac atcacgctcc
      181 tcgacctccg catgcccggc ctcgatggcg tcgccttgat cacggaactg cgccgcgaag
      241 atccagccgc ccgcttcatc gtcctgacca cctacgattc cgaccgcgat gtctcccgcg
      301 ccgtccaggc cggagcccgc ggttatctcc tcaaggacgc cccgaccgag gagctgctcg
      361 cctgcattca tgaggtctgc tccggaggca ccgccatcca ctccgccctc gtctcgaagc
      421 tcgccgccgg cctgcgcagc gatccgctta ccccgcgcga actcgatgtc cttcagctgc
      481 tcgccgaagg caaaagcaac cgcgaaatcg ccagcgggct tttcgtcggc gaggccaccg
      541 tgaagtccca tctgcaccgc gtcttcgcca agctcgacgt cctgagccgc accgaggccg
      601 tcgcggttgc cacccggcag gggctgattc ggctttaggt gctttccaag aaagcatttt
      661 gatagacgcg cggcgcgttt ttgacgcgat ccccatccgc atgcctcaac ttttcccgag
      721 ttcggcaaat acggtcactt atgcggtttt tggaaccgtc ctgctcggaa ccgccgcgct
      781 gatttgggcc gcggtcgaaa tcacgctctc cccctacttc acccgggtga atgtcccgat
      841 cggccagccc gtccccttca gccatatgca tcacgtggag gggctgggca ttgactgccg
      901 ttattgccat acgagcgtgg aggtcgccag ctcggcgggc attccctcga cggagacctg
      961 catgacgtgc cactcccagg tctggaagga cgcgccggtc ctccagccgg tgcgggacag
     1021 cttcatgacc gggaaggcgc tggaatggcg gcgggttcac aacctgcccg gcttcgtcta
     1081 tttcgatcac agcattcacg tgaaaaaggg ggtgagctgc atggactgcc acgggcggat
     1141 cgatgaaatg ccgctcgtct ccaagcagca tacgcttttc atgaaatggt gcctcgactg
     1201 ccatcggcat cccgaatcgg cgctcgttcc ccccgcggcg gtctttcaga tgcccgcgcc
     1261 gccgcccaga gaggcgcgga gcatttcccg ctggatggcg gagcagaacc cgcgcacccg
     1321 aaccatcatc acggattgca ccgcctgtca ccgatgaaaa ccgaaccacc gccgcgcgcc
     1381 gcctggatgg gaagatttgc cgatcaattc ccgcaatggc gaagcctgga gcaactggcc
     1441 gacacgccgg agtttcggga gttcctggag cgggaattcc ccgaggaggc ttcggaatgg
     1501 ccggacgcca tgagccggcg gcgctttctc ggcctgatgg gtgcctcgct tgccctggcg
     1561 gggttgtccg cctgcagccg ccagccgatt gaaaagctgg tgccctacgt ccgccagccg
     1621 cccgagctga cccccggcaa accgctttat tttgcgtccg ccgacctgct cagcggcgtt
     1681 gcccggggaa tcgtggtcga aagccacgag ggccgcccca cgaaaatcga ggggaatccg
     1741 cgccaccccg gcagcctcgg cgggacggat gccctcatgc aggcgtccat tctccggctc
     1801 tacgacccgg atcgctccaa gacgccgctg cgggagcgaa acgtttccac ctgggacgcg
     1861 ttcctggccg aatcgcaatc gcttcggaat caatgggcaa agacccaagg cgcgggtctc
     1921 cgcttcctca gcggccagac gacctcgccg gcgctgctgg atcaaatcca gcgattcctc
     1981 acgcaatttc ccgcggcccg ctggaccctt cacgagccgg ccgtcccggc gcatccgctt
     2041 caggccgtcg ccgatctgga ccgggcggac gtcattttgg ccatcgacgc ggattttctc
     2101 gtctgcgggc cggggcagct cgcctattcg cgacagtttg cccgccggcg cgatcccgag
     2161 gcggggatga accgcctcta cgtggccgag agtttcccca gcctcacggg ggccgccgcg
     2221 gaccaccggt tttcgctgaa accggccgat ctcgcccggc tgccggggtg gatcgaagac
     2281 gccctgtccg gagccccgga cccggacgcg ccgtcctggg tggcggcggt ggtccaagat
     2341 ctggaacggc acgcgggccg cggcctcgtg ctggcgggag attgcctgtc tgggacggcg
     2401 caggaggcgg tttatcggct caacgaccgg ctgggcaata tcggccagac cctccgctac
     2461 cagtcgaaat ggccggagcc cgcccacccg cttcccttgc gcgagctcgt gaaggaaatg
     2521 gcggcgggac gggtggagac gctgttcatt ctggacgcga atccggtcta tacctgcccg
     2581 gcggacgttc ccttcgccga ggcaatggcg aaggtgccgc tcacggtcca tcacgggctc
     2641 tacgtggacg aaaccgccgc ccgctgcgtc tggcatttgc cggaaaccga ttcgctcgaa
     2701 gcctggagcg acgccgcggc gtttgacggg a
//
LOCUS       DATDAY010000204         4134 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_1379908, whole genome shotgun sequence.
ACCESSION   DATDAY010000204 DATDAY010000000
VERSION     DATDAY010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 4134)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4134)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4134
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1379908"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(153..1229)
                     /locus_tag="VIS74_03990"
     CDS             complement(153..1229)
                     /locus_tag="VIS74_03990"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEY5792433.1"
                     /translation="MAHADGMTYAPQGKPAPVCQAGDFQFAAVHLDHGHIYGQCNGLI
                     EAGGEVTWVYDPDPAKVDAFRQKFPQARVAPSLEAVLDDPSIRLVAAAAITNRRGPLG
                     CRVMEAGKDYFTDKAPFTTLEQLAEARAITARTGRKYLVYYSERLHVECAVLAGQIIQ
                     DGAIGRVIQTLGLGPHRLNAASRPSWFFKHEEYGGILCDIGSHQCEQFLHYTGAKDAR
                     INTAHVANYAHPEYPEFEDFGELSLTADNGASGYMRVDWFTPDGLRTWGDGRTLILGT
                     KGYLELRKYVNVATADGTGDQLILVDAKGEQLIPCAGRVGYPFFGQLILDVLNRTENA
                     MTQEHTFKAAELCLKAQAAAVRIA"
     gene            complement(1231..2001)
                     /locus_tag="VIS74_03995"
     CDS             complement(1231..2001)
                     /locus_tag="VIS74_03995"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEY5792434.1"
                     /translation="MKVSQIACQLFTLRDFLKTPADIAATLKKVRAIGYEAVQVSGMG
                     PIPEAELVAICQGEGLTICGTHEPAVRILDETPAVVERLHKLGCIHTAYPFPSGVNFE
                     DPQSVQTLIEKLNAAGQVLHEAGLSLSYHNHGHEFRHLNGKPILQKIYEETDPRYLKA
                     ELDTYWVQFGGGDPVAWCRQFAGRLPWLHCKDYGISKKYEPVFTEVGYGNLNWPAILA
                     AAEEGGCGWFIVEQDVCPGDPFDSIRKSFEYLKTHFCN"
     gene            complement(2007..3161)
                     /locus_tag="VIS74_04000"
     CDS             complement(2007..3161)
                     /locus_tag="VIS74_04000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEY5792435.1"
                     /translation="MKTLRLGIIGIGNMGKNHANSLLKGNVPRCELAAVADANPAALA
                     LFPDTPHFADGSALIQSGTVDAVLIATPHYSHTTLGIEALEAGLHVLVEKPISVHKAD
                     CERLIAAHRPKKQVFAAMFNQRTDPYYLKLRELIRSGQLGEIRRVNWIITNWFRTYTY
                     YASGGWRATWAGEGGGVLLNQSPHQLDLWQWLFGPPQRLRAFCQVARYHEIEVEDNVT
                     AYMEYKNGATGVFITSTGEAPGTNRLEVTGEQGRVVIENDRFLFTRNEVPMTEFSKTS
                     GSGFAAPPIWNVEIPINGHGGQHDEILRNFTAAVLDKAPLIAPASEGIHSVELANAML
                     YSSFENKTVEFPLDAKAYEKLLKKKIASSTFVKKASKVKMDAADFSKSFR"
     gene            3240..>4134
                     /locus_tag="VIS74_04005"
     CDS             3240..>4134
                     /locus_tag="VIS74_04005"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEY5792436.1"
                     /translation="MKSPVSPNSPIRSTAQLAQLLGLSRWTVSRALNGHPGIHPETAE
                     RVREAARREGFAPNILGRGLRAGRTDLIGVCLPDLEDYFLAAKISRLQEAVVAQGLQP
                     LMQITRSEASESAALERFAAMRCGAVVLIASRLPDGHPALRHLAASGIQRVFIDPLHG
                     QSDAVVATDRALAMRHALGYLHELGHRGVAVAGINPAHPYGRQRERGLRQACRKCGWD
                     FSRQVVFLNRAEDLEDSHLGQKLAEDYLREHYPARRAIIALNDRIALGMMQRILKAGL
                     RIPGDVSILGYDDAAFAEVVSP"
BASE COUNT          691 a         1206 c         1435 g          802 t
ORIGIN      
        1 agaattgatt atcagtaatt aatctcacgc aaaggggcga agggagaaag cgggtgactg
       61 gtgactggcg caagaaacgt ctcttgcgaa aaaccatccc cctccgcaag tcaccagtca
      121 ctcgcttcct tctccgcgcg aggccaaaaa aatcaggcga ttcgcaccgc cgcggcctgg
      181 gctttcaggc acaactcggc ggccttgaac gtgtgctcct gcgtcatggc gttttccgtc
      241 cggttcagga cgtcgaggat caattggccg aagaagggat accccacccg gcccgcgcag
      301 ggaatgagct gctcgccctt cgcatccacg aggatgagct ggtcgcccgt gccgtcggcg
      361 gtggccacgt tcacgtattt gcgcagttcg aggtagccct tcgtgccgag aatgagcgtg
      421 cggccgtcgc cccaggtgcg caggccgtcc ggcgtgaacc agtccacccg catgtagccg
      481 gaggcgccat tgtcggcggt cagcgagagc tcgccgaagt cctcaaactc gggatattcc
      541 ggatgcgcgt agttggcgac gtgggcggtg ttgatccgcg catccttcgc gccggtgtaa
      601 tgcaggaact gctcgcattg atggctcccg atgtcgcaaa ggatgccgcc gtattcctca
      661 tgcttgaaaa accagctggg ccgcgacgcc gcattcaggc ggtgcggtcc gaggccgagg
      721 gtctggatga cgcggccgat ggcgccatcc tgaatgatct gccccgcgag cacggcgcat
      781 tccacgtgca gccgctcgct gtaatacacc aggtatttgc gcccggtgcg ggccgtgatc
      841 gcgcgcgctt ccgcgagctg ttccagcgtg gtgaagggcg ccttgtcggt gaaataatcc
      901 ttgccggctt ccatgacccg gcagccgagc gggccgcggc ggttggtgat ggcggccgcg
      961 gcgacgagcc ggatcgaggg atcgtccaga acggcttcca aactgggcgc gaccctggcc
     1021 tgcgggaact tctggcgaaa ggcgtccacc ttggccggat cgggatcgta aacccaggtg
     1081 acctcgccgc cggcctcgat caggccgttg cattgcccgt agatgtggcc gtggtcgaga
     1141 tgaaccgcgg cgaattggaa atcgcccgcc tggcaaaccg gagcgggttt gccctggggc
     1201 gcgtaggtca tgccgtccgc gtgcgccatg ttagttgcag aagtgggttt tgaggtattc
     1261 gaagctcttg cggatcgaat cgaacggatc gccggggcag acgtcctgtt cgacgatgaa
     1321 ccagccgcag ccgccctctt cggcggcggc gagaatggcc ggccagttca ggttgccgta
     1381 gccgacttcc gtgaagacgg gctcgtattt tttcgagatg ccatagtcct tgcagtgcag
     1441 ccagggcagg cggccggcga actggcggca ccaggcgacc gggtcgcccc cgccgaactg
     1501 aacccagtag gtgtccagct ccgccttcag gtaacgcggg tcggtttcct cgtagatttt
     1561 ttggaggatg ggcttgccgt tcaggtggcg gaattcgtgg ccgtggttgt gataggaaag
     1621 cgagagcccc gcctcgtgca gcacctggcc ggcggcattg agtttttcga tgagcgtctg
     1681 gaccgactgc ggatcttcaa agttcacgcc ggaggggaag ggatacgccg tgtggatgca
     1741 gccgagcttg tgcaggcgtt ccacgacggc cggcgtctcg tcgagaatcc ggaccgccgg
     1801 ttcgtgcgtg ccgcagatcg tcagtccttc gccctggcag atggccacga gttccgcttc
     1861 cggaatcggc cccatgccgc tgacctggac ggcttcgtag ccaatggccc gcaccttctt
     1921 gagggtggcg gcgatgtcgg ccggggtttt gaggaaatca cgcagggtga aaagctggca
     1981 ggcgatttgg gagactttca tggggattag cggaaggatt tggaaaagtc agcggcgtcc
     2041 attttgacct tggacgcctt tttgacgaac gtggaggagg cgatcttttt ttttagcagc
     2101 ttttcgtaag ccttggcatc gagcggaaac tccacggttt tgttttcaaa gctggaataa
     2161 agcatcgcgt tggccagttc cacggagtgg atgccctcgc tggcgggggc gatgagcgga
     2221 gccttgtcga gcacggccgc ggtgaagttg cgcaggattt catcgtgctg gccgccgtgg
     2281 ccgttgatcg ggatttcgac gttccagatc ggcggcgcgg cgaaaccgct gccgctcgtc
     2341 ttggaaaatt ccgtcatggg aacctcgttg cgggtgaaga ggaaccggtc gttttcaatg
     2401 accacgcggc cctgttcgcc ggtgacctcg agccggttgg tgcccggcgc ctcgccggtg
     2461 gaggtgatga agacgccggt cgcgccattc ttgtattcca tgtaggcggt gacgttgtcc
     2521 tcgacctcga tctcgtgata gcgggcgacc tggcagaacg cgcgcaggcg ctggggcggg
     2581 ccaaagagcc actgccagag gtcgagctgg tgcgggctct ggttgagcag cacgccgccg
     2641 ccctcgccgg cccaggtggc gcgccagccg ccggaggcgt agtaggtgta ggtgcggaac
     2701 caattggtga tgatccaatt cacgcgccgg atttccccga gctggccact gcggatcaat
     2761 tcgcggagct tgaggtaata gggatcggtg cgctggttga acatggccgc gaagacctgt
     2821 tttttcgggc ggtgcgcggc gatcagcctt tcgcaatccg ccttgtgcac cgagatgggt
     2881 ttctccacca gcacgtgcag gcccgcctcg agggcctcga tgccgagcgt ggtgtgggaa
     2941 taatgcggcg tggcgatcag gaccgcgtcc accgtgccgg actggatcaa ggcgctgccg
     3001 tcggcaaaat gcggcgtgtc cgggaagagc gccagcgcgg cgggattggc atccgccacc
     3061 gccgcgagct cacagcgggg aacgtttccc ttgagcaggg agttggcgtg gtttttgccc
     3121 atgttgccaa tgccgataat gccgagacgc aatgttttca tgaaggaagg gagagagagg
     3181 gacggggttg cttgactgag ttaccaacac gtgttagtat gaaagcccgc gcccgtcaaa
     3241 tgaaatcccc cgtttcccca aattccccga tccgctccac ggcccagctg gcgcagctgc
     3301 tgggcttgtc gcgctggacc gtgtcccgcg cgctcaacgg gcatccgggg attcatccgg
     3361 agacggcgga gcgggtccgg gaggccgcgc gccgggaggg ctttgcgccg aacatcctgg
     3421 gccgggggct ccgggcgggc cggacggatc tcatcggggt ctgcctgccg gatttggagg
     3481 attattttct ggccgccaaa atctcgcggc tgcaggaggc ggtggtggcg caggggcttc
     3541 agcccctcat gcagatcacc cgcagcgagg ccagcgagag cgccgcgctg gagcggtttg
     3601 cggccatgcg gtgcggggcc gtggtgctga tcgcctcccg gctgccggac gggcatccgg
     3661 cgctgcggca cctcgcggcc tccggcatcc agcgggtgtt tattgatccg ctgcacggcc
     3721 aatccgacgc ggtggtggcg accgaccgcg ccctggccat gcggcacgcg ctggggtatc
     3781 tgcacgagct gggacaccgc ggcgtggcgg tggccgggat caatccggcc catccctacg
     3841 gccggcagcg ggaaaggggc ttgcggcagg cgtgccggaa atgcggttgg gatttttccc
     3901 ggcaggtggt tttcctcaat cgcgcggagg atttggagga ttcgcatctg gggcagaagc
     3961 tcgcggagga ttatttgcgg gagcattacc cggcccggcg ggccattatt gcgctgaatg
     4021 atcgcatcgc cctcgggatg atgcagcgaa tcctgaaggc cgggctgcgc attcccgggg
     4081 acgtgtcgat cctgggttac gatgacgccg cgtttgcgga ggtggtatcc ccgc
//
LOCUS       DATDAY010000205         5145 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_1260087, whole genome shotgun sequence.
ACCESSION   DATDAY010000205 DATDAY010000000
VERSION     DATDAY010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 5145)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5145)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5145
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1260087"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1156
                     /locus_tag="VIS74_04010"
     CDS             <1..1156
                     /locus_tag="VIS74_04010"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF022449.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY5792437.1"
                     /translation="TRRHPGAPVLMTEATHRIGDVMLHNSVNVMLRQREADKILDYPL
                     FTHRGVELSRQKWLRCPLRRPLSLSGERAASGEATTVEFFDAGHILGSTGVLIRHQGR
                     TLFYTGDVHFDDQTLMRGADFPASGVDVLVMETTRGDSPVPPGFTRAAEEARFGQAIL
                     DAFARGSCVTIPVFALGKTQELLAMLWKLRHQGTLPSTPIYIGGLSTKITEVYDALAA
                     SSQRSLADLQLLQEVAPYVVSGNDVANLSPRKRCIFALSSGMMTERTLSNFFARKVLE
                     DESQHLFFVGYSDPESPAGRIKAARRGDAVILDENLPPLRLRCQVDQFNFSAHATRDS
                     LRDYAIKLKPKKILLVHGDAPAVAWFRRELSAALPETEILVPEPGKTLSL"
     gene            1355..2914
                     /locus_tag="VIS74_04015"
     CDS             1355..2914
                     /locus_tag="VIS74_04015"
                     /inference="COORDINATES: protein motif:HMM:NF037979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HEY5792438.1"
                     /translation="MSTPAKEQWSSRIGVILAVTGSAVGLGNFLRFPGLAAQYEGGIF
                     MIPYFVSLLVIGLPLAWAEWAMGRYGGVRGFNSTPGIFRLIWKSPLAPYVGVLGLIVP
                     VMIYMYYVFIEAWCLGYAWNYLAGSLSLGKSADAYGGFFAQYTGIAGDGSLFQSGHGS
                     ALVFLVICILINFWIIYRGLSRGIELFCLWAMPVLVILALIVLVRVLTLGTPDPAHPE
                     LNVLNGLGFMWNPQAGGTAFWKALFNAEMWLKAAGQIFFSLSVGFGVIITYASYLKKS
                     DDVALSSTTAVAGNEFCEVSLGGMITIPAAFVFLGAAGAAGGTFALGFKTLPMVFEYM
                     PAGRVFGFFFFFLLFLAAITSSLSMLQPAIAFLEEGLALNRKTSVIFLGFITLAGNGI
                     VVYFSGNAAALGALDFWVGTLCIFLLATVQTILFSWVLGAEKGMEELRAGAELAIPRG
                     FIPLIKYVSPVYLITIFIGWLITDFRKEVAGTLSSLGGIVALVFIAICFALFLLLINQ
                     AVIRWNQKANP"
     gene            2911..3063
                     /locus_tag="VIS74_04020"
     CDS             2911..3063
                     /locus_tag="VIS74_04020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792439.1"
                     /translation="MTLGGWIIMLLSVGGTTGWAIWCVCRVLSKPKSAGKLHGQMDIH
                     LPDQDR"
     gene            3060..3815
                     /locus_tag="VIS74_04025"
     CDS             3060..3815
                     /locus_tag="VIS74_04025"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEY5792440.1"
                     /translation="MTGPALSPGEWAVAGLAAVCIGLSKSGFGGVGIVAIVGMAAILP
                     PRESTGTILPMLICADVFAVWQFRRHVQWRYVFRTAIPAILGVLAGFWLMPAISDAAF
                     GPFIGWLTLALIGMLILLKANAFLRNLPLRYPAVFGWPMGFLAGATTMLANAAGPVMS
                     VYLLASRLPKMEFVGTAAWFFFAINLFKVPFSAALGLIHPQSLTLNLCLLPGVVAGVF
                     LGKLLLGKISQTFFEWLMLGFAILGGLKLVIFG"
     gene            3868..>5145
                     /gene="thrS"
                     /locus_tag="VIS74_04030"
     CDS             3868..>5145
                     /gene="thrS"
                     /locus_tag="VIS74_04030"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEY5792441.1"
                     /translation="MTDLERIRHSCAHVLATAILRLWPEAQFAAGPPVENGFYYDVEL
                     PHRITPEDFARIEAEMKKEIKANHVFERVEVSREEVIQLAKDGRLAALGARPEASKFK
                     LDIVERIPEGETITLYRNGDFTDLCAGPHVLRTGNIGAFRLTHVASAYYKGDENNPQL
                     QRIYGTAFKNKTAMDEYFAMLEEAKKRDHRKLGAEMGLFAMDPEYVGPGLPLWLPKGT
                     VIVEELEKLAKETEFAAGYVRVRTPHLAREKMYKTSGHLPYYAESMFPPMELDESAGS
                     DSENPKSEIQNPKSTDRYYLKAMNCPHHHRIFAAEPRSYRDLPLRLAEYGCCYRYEQS
                     GELFGLMRVRSLNMNDAHIYCTEEQFAEEFRAVNEMYLKYFKIFGLEKYQMRFSTHDP
                     EKLGQKFVDEPELWKKTEDMVRNVLIESGINFVE"
BASE COUNT          900 a         1670 c         1514 g         1061 t
ORIGIN      
        1 gacccgccgg cacccagggg cgccggtgct gatgaccgag gccacgcacc ggatcggcga
       61 cgtcatgctg cacaactccg tgaacgtgat gctccgccag cgcgaggccg acaagattct
      121 cgattacccg cttttcaccc atcgcggcgt ggagttgagc cggcaaaaat ggctgcgctg
      181 ccccctgcgg cgccccctct cgctttccgg cgaacgcgcc gcgtccggcg aagcgaccac
      241 ggtggaattt tttgatgccg ggcacattct gggttccacc ggcgtgctga tccggcatca
      301 gggacggacc cttttttaca ccggcgacgt gcatttcgat gaccagaccc tcatgcgggg
      361 cgcggatttt ccggccagcg gggtggatgt gctcgtcatg gaaacgaccc ggggcgattc
      421 gccggtcccg ccggggttta cccgggccgc cgaggaggcg cggttcggcc aggcgatttt
      481 ggacgccttt gcgcggggct cctgcgtgac gattcccgtc ttcgcgctcg gcaagaccca
      541 ggaattgctc gcgatgctct ggaaactccg gcaccagggc acgctgccca gcacgcccat
      601 ctatatcggc ggcctgagca cgaagattac cgaggtttac gacgcgctgg cggcgtccag
      661 ccagcgctcc ctggcggatt tgcaactgct ccaggaagtg gcgccctacg tggtttccgg
      721 caacgacgtc gccaatctca gcccgcggaa acgctgcatc ttcgcccttt ccagcggcat
      781 gatgaccgag cgcacgcttt ccaatttttt tgcgcgcaag gtgctcgagg acgaatccca
      841 gcatcttttc ttcgtgggat attccgatcc cgagtcgccg gccggccgga tcaaggcggc
      901 ccggcgcggg gatgcggtga tcctcgacga aaacctgccg ccgctgcggc tgcgctgcca
      961 ggtggaccaa tttaatttta gcgcccacgc gacgcgcgac agcctgcggg attacgcgat
     1021 caagttgaaa ccgaaaaaaa tcctgctcgt ccatggcgat gctcccgcgg tcgcgtggtt
     1081 tcggcgggaa ctttcggccg cattgccgga gacggaaatc ctggtgccgg aacccggaaa
     1141 aacgctgtcc ttgtaaatcc attgggcggg gctcccctcc gcccgtttct caaacaaaac
     1201 gaaaccccga aaaagttaca gcccatcgct gcgaaagcaa gcgatgggct gttgttcccc
     1261 ccagaacagt tttcacctta gcaaagtctt tctgactggc aagggaaaaa ataaactttt
     1321 ttctttcgcc gcggtaactt aaacgcattc atcgatgtcc acaccggcca aagagcaatg
     1381 gagttcgcgc atcggggtca tcctggcggt gaccggaagc gcggtggggc tgggcaattt
     1441 ccttcgcttc cccggtctcg cggcgcaata cgagggcggc atcttcatga ttccctactt
     1501 cgtctcgctg ctggtcatcg gcctcccgct ggcctgggcg gaatgggcga tgggccgcta
     1561 cggcggcgtt cgcggattta attccacccc gggcattttc cgcctcatct ggaaaagccc
     1621 gctcgcgcct tatgtcggcg tgctgggatt gatcgtcccg gtgatgattt acatgtatta
     1681 cgtgttcatc gaggcatggt gcctcggcta cgcgtggaac tacctcgcgg gcagcttgtc
     1741 gctcggcaag agcgcggacg cctacggcgg tttttttgcg caatatacgg gcatcgccgg
     1801 cgacggctcc ttgtttcaat ccggccacgg ctccgcgctc gttttcctgg tcatttgcat
     1861 cctcatcaat ttctggatca tctaccgcgg cctgagccgc ggcatcgagc ttttctgcct
     1921 ttgggcgatg ccggtgctgg tgattctcgc gctgatcgtc ctcgttcgcg ttctcacgct
     1981 gggcacgccc gacccggccc atccggaact caacgtcctc aacggcctcg gtttcatgtg
     2041 gaatccgcag gccgggggaa ccgccttctg gaaggccctc ttcaatgccg aaatgtggct
     2101 caaggccgcc ggccagattt tcttttccct ttccgtgggc ttcggcgtca tcatcaccta
     2161 cgcgagttat ttgaaaaaaa gcgatgacgt cgccctgagt tccaccaccg cggtggcggg
     2221 caatgaattt tgcgaagtca gcctcggcgg catgatcacc attcccgcgg cttttgtgtt
     2281 cctgggcgcc gccggcgcgg cgggcggcac ctttgccctg ggtttcaaaa ccctcccgat
     2341 ggtgttcgaa tacatgcccg ccggccgcgt cttcggcttt ttctttttct tcctgctctt
     2401 cctggcggcg atcaccagct cgctgagcat gcttcagccc gccatcgcgt ttctggagga
     2461 gggactcgcg ctcaaccgca agacgtccgt cattttcctc ggcttcatca cgctggcggg
     2521 caatggcatc gtcgtttatt tcagcggcaa cgcggccgcg ctcggcgccc tggatttctg
     2581 ggtcggcacg ctgtgcatct tcctgctcgc caccgtgcag acgatcctct tcagctgggt
     2641 gctcggcgcg gaaaagggaa tggaggaact gcgcgccggc gcggagctgg cgatcccccg
     2701 cgggttcatt ccgctcatca aatacgtttc gccggtttat ctgatcacca ttttcatcgg
     2761 ctggctgatc accgatttcc gcaaggaagt cgccggcacc ctcagctcgc tcggcgggat
     2821 cgtggcgctg gtcttcatcg ccatttgttt tgcgctgttc ctgctcctca tcaaccaggc
     2881 ggtcatccgc tggaaccaaa aggccaaccc atgacgcttg gcggctggat catcatgctt
     2941 ctttccgtcg gcggcaccac cggctgggcc atttggtgcg tctgccgcgt gctgagcaag
     3001 ccgaagtccg cgggaaaact ccacgggcag atggatatcc acctccccga tcaggaccga
     3061 tgaccgggcc cgcgctgagc cccggggagt gggcggttgc ggggctggcg gccgtttgca
     3121 tcggcctgag caagtccgga tttggcggcg tgggcattgt cgccatcgtc ggaatggccg
     3181 ccatcctgcc gccgcgcgaa tccaccggga cgatcctgcc gatgctcatc tgcgcggacg
     3241 tgttcgccgt ctggcagttc cgccgtcatg tccagtggcg ctacgtgttc cgcacggcca
     3301 ttcccgcgat cctcggcgtc ctggccggat tctggctcat gccggcgatc tccgacgccg
     3361 cgttcggccc cttcatcggc tggctcacgc tcgcgctcat cggcatgctc atcctgctca
     3421 aggccaatgc ctttctgcgc aacctcccgc tgcggtatcc ggccgtcttc ggctggccga
     3481 tgggtttcct cgccggcgcc accacgatgc tggccaacgc cgccgggccg gtcatgagcg
     3541 tttatctgct ggccagccgg ttgccgaaaa tggagttcgt tggcaccgcc gcgtggtttt
     3601 tcttcgccat caatctcttc aaggtcccct tcagcgccgc gctcgggctc atccacccgc
     3661 agagcctcac gcttaatctc tgcctgctgc ccggcgtcgt ggccggcgtc ttcctcggga
     3721 agctcctgct cggcaaaatt tcgcaaacct ttttcgagtg gctcatgctg ggcttcgcca
     3781 tcctcggcgg gctgaagctg gtaatctttg gctgattgcc aaaagccgga cggcgtgatt
     3841 ccattgcggg ttccgatgac tcccgtaatg accgatctcg aacgcatccg ccactcgtgc
     3901 gcccatgtgc tggccacggc catcctgcgc ctgtggcccg aggcgcaatt cgccgccggc
     3961 ccgccggtgg agaacggctt ttattacgac gtcgaattgc cccaccgcat cacgcccgag
     4021 gacttcgcgc ggatcgaggc ggagatgaag aaggagatca aggcgaacca cgtcttcgag
     4081 cgcgtggaag tctcgcggga ggaagtcatt caactcgcga aggacggccg gctcgccgcg
     4141 ctgggcgcgc gcccggaggc gagcaagttc aagctcgaca tcgtcgagcg cattccggag
     4201 ggcgaaacga tcacgctgta tcgcaatggc gatttcaccg acctctgtgc cggcccgcac
     4261 gtcctgcgca ccggcaacat cggcgcgttc cggctcaccc atgtcgccag cgcttattac
     4321 aagggcgacg aaaacaatcc ccagctccag cgcatctacg gcaccgcctt caagaacaag
     4381 accgcgatgg acgaatactt cgccatgctc gaggaggcga agaagcgcga ccatcgcaag
     4441 ctcggcgccg agatgggcct cttcgcgatg gaccccgaat acgtcggccc cggcctcccg
     4501 ctctggctgc caaaaggcac ggtcatcgtc gaggagctgg aaaaactcgc gaaggaaacc
     4561 gaattcgccg ccggctacgt ccgcgtccgc accccgcact tggcgcggga gaagatgtat
     4621 aagacgagcg gccatttgcc ctactacgcg gagagcatgt ttccgccgat ggaactcgat
     4681 gagagcgctg gatcggattc agaaaatccg aaatccgaaa tccaaaatcc aaaatcgacc
     4741 gaccgctact acctcaaggc catgaactgc ccgcaccacc accgcatctt cgcggcggag
     4801 ccgcggagtt atcgcgacct gccgttgcgc ctcgcggagt atggctgctg ctaccgctac
     4861 gagcagagcg gcgagttgtt cggcctcatg cgcgtgcgtt cgctgaacat gaacgacgcg
     4921 cacatctatt gcaccgagga gcagttcgcc gaagagttcc gtgcggtgaa tgagatgtat
     4981 ctcaaatact tcaaaatttt cggcctcgag aaataccaga tgcgcttctc cactcacgac
     5041 cccgagaagc tcggccagaa attcgtggac gagcccgaac tgtggaaaaa gaccgaggac
     5101 atggtgcgaa atgtcctcat cgagagcggc atcaacttcg tcgaa
//
LOCUS       DATDAY010000206         2077 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_2520655, whole genome shotgun sequence.
ACCESSION   DATDAY010000206 DATDAY010000000
VERSION     DATDAY010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2077)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2077)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2077
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2520655"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..726
                     /locus_tag="VIS74_04035"
     CDS             <1..726
                     /locus_tag="VIS74_04035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792442.1"
                     /translation="YFQRIDFFEKLGVSTSEFFHRHPPTNRFVPIQKITFNSDSGTIA
                     TSLSNCIAGQASSQDESYFLVQYATGEIISNCKQHSGGQGFASAQYFKKHDLARFGVG
                     DSGKGIRESFRVNQSPHYQEEMTDETAILLALKPEVSSVTHLRNVYEGRSNRGLGLSV
                     LRELVKHTHGYLSVISGNGWYFQDGNLPGKSGTMRNGCSYHGTLVSAAFDRGQVANYN
                     DLHNEALRALGLQISIPDVLRFT"
     gene            723..1037
                     /locus_tag="VIS74_04040"
     CDS             723..1037
                     /locus_tag="VIS74_04040"
                     /inference="COORDINATES: protein motif:HMM:NF025574.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS-like domain-containing protein"
                     /protein_id="HEY5792443.1"
                     /translation="MKQAFQMANLFSSFLADGEIGNRFRFTEVETAFTLGNVVVFDFV
                     GVTNMTDSFANGLFANLMADHPNDFANLIEFKNCSPLIRSLITSAVARGWEESKNWPL
                     TN"
     gene            complement(1030..1485)
                     /locus_tag="VIS74_04045"
     CDS             complement(1030..1485)
                     /locus_tag="VIS74_04045"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002855998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase"
                     /protein_id="HEY5792444.1"
                     /translation="MKIAIGSDHAGYAYKQRLIEFLQQEGHEVVDFGTHSTESTDYPL
                     YIIPTAEAVVRGEAERGIVLGGSGNGEAIAANKVKGVRAALCWNLQTAKFSRMHNDAN
                     VLSLGERMVSIEIALDIVKLWLETPFEGGRHVKRIEEMSRYENGEGLFS"
     gene            complement(1482..>2077)
                     /gene="bioB"
                     /locus_tag="VIS74_04050"
     CDS             complement(1482..>2077)
                     /gene="bioB"
                     /locus_tag="VIS74_04050"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HEY5792445.1"
                     /translation="EVCVTLGQLGDEEARRLKEAGVTAYNHNIDTSPEHYETIVSTHT
                     YQDRLNTIRSVQKAGMAVCCGGIIGLGETEDDRLRMLEVLCSFDPPPESVPINYLVPI
                     PGTPLEQSKPVDLFEMIRLIAATRIALPNAKVRLSAGRRSLTREAQAMCFFAGANSIF
                     YGDKLLTTANPQANEDLELLEKLDLKPLEPAVESLVS"
BASE COUNT          452 a          585 c          537 g          503 t
ORIGIN      
        1 tactttcaaa gaattgattt cttcgaaaaa ctcggagtat ccacctcgga gttttttcat
       61 cgccatccac ccaccaaccg cttcgttcca atccaaaaaa tcacattcaa ttcagattcc
      121 ggaacgattg ccacctcttt atccaattgc atcgccggac aagcctcttc acaggatgaa
      181 tcatactttc tcgtccaata tgcgaccgga gagatcattt ccaactgcaa gcaacattcc
      241 ggcggacaag gctttgcctc agcccaatat ttcaaaaagc acgatctggc tcgctttggc
      301 gtcggtgaca gcggcaaagg cattcgtgag agttttcggg tcaatcagtc tcctcattat
      361 caagaggaaa tgacagatga aaccgccatt cttcttgccc taaaaccaga agtttcatct
      421 gtcacgcatc tccgcaatgt ttatgagggt cgttcaaatc gaggactcgg attaagcgtt
      481 ctgcgggagc tggtaaaaca tactcatggt tatctttccg tcatcagtgg aaatggctgg
      541 tacttccagg atggaaatct acccgggaaa agcggaacca tgcgcaatgg atgcagctat
      601 catggcaccc ttgtttcagc cgcttttgat cgtggccaag ttgcaaacta caatgacttg
      661 cacaatgaag cgttgcgcgc cttgggcttg caaatttcaa ttcctgatgt attaaggttt
      721 acatgaaaca ggctttccag atggccaatc tctttagttc tttcttagcc gatggagaaa
      781 ttggcaatcg ttttcgtttt accgaagttg aaactgcgtt tactttgggt aatgtggtcg
      841 tattcgactt tgtgggcgtg acaaacatga ctgactcctt tgcaaatggc cttttcgcca
      901 atctaatggc cgatcatccc aacgattttg caaatctgat cgaattcaaa aattgcagtc
      961 cgcttatccg ctcgctaatt acatccgccg ttgctcgcgg ttgggaagag tcgaaaaatt
     1021 ggccgttaac taactgaaaa gcccctcgcc gttctcgtaa cggctcattt cctcgatccg
     1081 cttcacgtgg cggccgccct caaatggcgt ttccagccag agctttacga tgtcgagcgc
     1141 gatctcgatc gataccatgc gctcgcccag cgagaggacg ttcgcatcat tgtgcatgcg
     1201 ggaaaatttc gccgtctgca aattccagca tagcgcggcg cgcacgccct tgaccttgtt
     1261 cgcggcgatc gcctcgccat tgcccgatcc gcccagcacg atgccgcgct cggcctcgcc
     1321 ccgcacgacg gcctccgccg tgggaatgat gtaaagcggg taatccgtgg actcggtgct
     1381 gtgcgttccg aagtccacga cctcatgccc ctcctgctgg aggaactcga tgaggcgttg
     1441 tttgtaagcg tatccggcgt ggtcggaacc gatggcgatt ttcatgaaac gagggattcc
     1501 acggcgggtt cgagcggttt gaggtcgagc ttttcgagca gctccagatc ctcattcgcc
     1561 tgcgggttgg cggtggtgag cagcttgtcg ccgtaaaaaa tggaattcgc cccggcaaag
     1621 aaacacatcg cctgcgcctc gcgcgtgagg ctgcggcggc cggcggaaag gcggaccttg
     1681 gcgttcggca gcgcgatgcg cgtggctgcg atcaggcgga tcatttcaaa caaatccacc
     1741 ggcttcgact gttccagcgg cgtgccggga atcggcacga ggtaattgat gggcacgctc
     1801 tccggcggcg gatcgaagct gcacagcacc tccagcatgc gaagccggtc gtcctcggtt
     1861 tcgcccaggc caataatgcc gccgcagcaa accgccatgc ccgccttctg cacgctgcgg
     1921 atggtattca gccgatcctg atacgtgtgc gtcgaaacga tggtttcgta gtgttccggc
     1981 gacgtatcga tgttgtgatt gtaagccgtc acccccgcct ccttgagccg ccgggcctcc
     2041 tcgtcgccga gctggccgag ggtcacgcag acttcca
//
LOCUS       DATDAY010000207         2097 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_341655, whole genome shotgun sequence.
ACCESSION   DATDAY010000207 DATDAY010000000
VERSION     DATDAY010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2097)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2097
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_341655"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..348
                     /locus_tag="VIS74_04055"
     CDS             <1..348
                     /locus_tag="VIS74_04055"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEY5792446.1"
                     /translation="NDRSALKAVIRKIQEGNGVVLFPEGTRSPDGQLQPGQPGIGLIV
                     AKTLAPVVPMRIFGAYEAFPKAAKGVKFRRITVVVGEPLHFTAEDVGAGTREDYRRLS
                     QRIMESIGNLELP"
     gene            345..548
                     /locus_tag="VIS74_04060"
     CDS             345..548
                     /locus_tag="VIS74_04060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792447.1"
                     /translation="MNPVTGEQPVAAFSWGAGVLLLLIGLQAAAILQIPPKNETGVRV
                     IPWKAPDLEIAAPEPEKLPEYLP"
     gene            720..1562
                     /locus_tag="VIS74_04065"
     CDS             720..1562
                     /locus_tag="VIS74_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HEY5792448.1"
                     /translation="MAIEDTDSGFQQYLRDIAQFPLLTREEEVKLARRIHKGDQEARA
                     EMVRANLRLVVKIARDYANYGLPLLDLISEGNIGLMKAVERFDPSKGGKLSTYAAWWI
                     KQSIKRALANQGKTIRLPVHLVDKISKIRRVATQMMEELGREPTDDELAEEIGLSAAK
                     VSALKAAAIRPTSLDAPIGEDDSTEFGEIVGDEEAQDPFEMLRDKDLRDEVGDLLDLL
                     DDRERRIINSRFGLDGSKPKTLEEVGEKFGVTRERIRQLQNIALNKLRKGLSKKERPK
                     LELA"
BASE COUNT          456 a          589 c          641 g          411 t
ORIGIN      
        1 aacgaccgct cggctctgaa ggcggtgatc cggaaaattc aggagggcaa tggcgtcgtg
       61 ctttttccgg aaggcacgcg ctccccagac ggccaattgc aacccggcca gcccggcatc
      121 gggctgatcg tggcgaaaac gctggcgccg gtggtgccga tgcggatttt cggagcttac
      181 gaggcgtttc ccaaggcggc caaaggcgtc aagtttcgcc gcatcacggt ggtcgtcggc
      241 gagccgcttc actttaccgc cgaggatgtc ggcgcgggca cgcgggagga ttatcggcgc
      301 ctcagccagc gcatcatgga atccatcggc aacctcgaac tgccatgaac ccggtcacag
      361 gggagcaacc ggtagccgcg ttttcctggg gagcgggagt gctcttgctc ctcatcggcc
      421 tacaggcagc ggcgattttg caaattcccc caaagaatga aaccggggtc cgtgtgatcc
      481 cctggaaagc gcccgatctg gaaatcgccg ctcccgagcc ggaaaaatta ccggagtatc
      541 ttccttagga ataggagatt gcatcatttt atccggcttt gaaaaagatt ttgaacggaa
      601 gctcgctctc cgggtctaaa tagtcaatga tcttaagtaa ttgggccgcc tgataaataa
      661 ggtgaaacca aaggcgaagt tggatcgtta tatctataaa ggacaaggga gaaaataaaa
      721 tggctatcga agataccgat tctggttttc agcaatacct gcgggatatt gcgcagtttc
      781 cgcttctgac ccgcgaggag gaagtcaagc tggcgcgccg cattcacaag ggcgaccagg
      841 aagcccgggc tgaaatggtc cgcgccaacc tgcgcctggt cgtcaagatt gcgcgggatt
      901 atgcgaatta cggcctgcca ttgctcgatc tgatctccga aggcaacatc ggcctcatga
      961 aggcggtgga gcgtttcgac ccgagcaagg gcggcaagct cagcacctac gcggcgtggt
     1021 ggatcaagca atccatcaag cgcgcgctcg ccaatcaggg caagacgatc cgcctgccgg
     1081 ttcacctcgt ggataagatt tcgaaaatcc gccgcgtggc cacgcagatg atggaagaat
     1141 tgggccgcga gccgaccgac gacgaattgg cggaggagat cggcctttcg gcggcgaaag
     1201 tttccgcgct caaggcggcg gccatccggc cgacttcatt ggatgcgccg atcggcgagg
     1261 atgactccac ggagttcggc gagatcgtcg gcgacgagga agcccaggat ccgttcgaga
     1321 tgctgcgcga caaggatctc cgcgacgaag tcggcgacct gctcgacctg ctcgacgacc
     1381 gcgaacgccg gatcatcaat tcccgcttcg ggctcgacgg ctccaagccg aagacgctcg
     1441 aggaagtcgg cgagaaattc ggcgtgaccc gcgagcgcat ccgccagctg cagaatatcg
     1501 cgctgaacaa gctgcggaag ggcctgagca aaaaggaacg gcccaagctt gagctggcct
     1561 gagcgaacga aacgggattt actggattga caggaaaagg agaggaatca cctcattttt
     1621 cccctccggt tatagtattt ctcaaaagag atttccgaaa tacccggcgg gttgtagagg
     1681 cgcttgtcgg gtggggaaat ggggccccac caccccattg ggcgcctttg gttgcgggga
     1741 aacggggacc ccgcatcctc gtttacgctg ggcggttggc acaattgccg cacttcgtgc
     1801 gcccctcggg ctgtcccgct tccgcgggac aggctgtctt cgctccggct gcccctccat
     1861 tgaatccctt ttcggatatc tcttttgaga atcgctctaa tccgaaaaat ccggtaatcc
     1921 agtttagttg gagaatttac ggaatcggaa actgcgcggt gagcgccgcg accttatggc
     1981 ggatgaccgc cagcgctgag tcgttgttgc ggacatcgag cgccgcggaa atcaggtcgg
     2041 cgagttccat catttcctct tctttcatgc cgcgcgtggt catcgcgggc gtgccga
//
LOCUS       DATDAY010000208         7487 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_1267734, whole genome shotgun sequence.
ACCESSION   DATDAY010000208 DATDAY010000000
VERSION     DATDAY010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 7487)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7487)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7487
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1267734"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..940)
                     /locus_tag="VIS74_04070"
     CDS             complement(<1..940)
                     /locus_tag="VIS74_04070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792449.1"
                     /translation="MFAEIEAWVGSWRQLFNRSEWIIRQLRLPVSKGTEEEPGLLLIQ
                     VDGLSRRQLEKAIHEGRLPFLKSLCRREEHELLTFYSGLPSTTPAVQAELYFGVRGGV
                     PAFSFRDPDSGEITSLFNPELARQLERQFARHGQGLLTGGSSWSNIFSGGAASGETHF
                     CISRMGIGDIWRRSHLLTLFTVLILHPISVLRVIALVLLEFAIGLTDALAGMCRGYSP
                     LPEMATVLSRMCAGIALREVITVGGKVDLARGLPVIHLNFVGYDEMAHRRGPAGRFSH
                     WSLLGIDRSIRDLHRAAHASRRRDYHVWIFSDHGQEA"
     gene            complement(944..1156)
                     /locus_tag="VIS74_04075"
     CDS             complement(944..1156)
                     /locus_tag="VIS74_04075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792450.1"
                     /translation="MSKSLMQKAEEAPPGHCRIVFHDRHGMPWKMAIHYPDQPVREYP
                     VKGMGLCDFHQQKAHLLRDHPGWRVE"
     gene            complement(1386..1715)
                     /locus_tag="VIS74_04080"
     CDS             complement(1386..1715)
                     /locus_tag="VIS74_04080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792451.1"
                     /translation="MAGVCLLGCSRPANEYSLSRQAVLSQESSLRGEIYIQEGANGPY
                     QVGPGFVGTIGSAGSITVPADQGPKKFSYPATPGYFFEGTAYLNKDRKAFVVIRRVPA
                     SAVEAEK"
     gene            complement(1819..2307)
                     /locus_tag="VIS74_04085"
     CDS             complement(1819..2307)
                     /locus_tag="VIS74_04085"
                     /inference="COORDINATES: protein motif:HMM:NF025658.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4375 domain-containing protein"
                     /protein_id="HEY5792452.1"
                     /translation="MKFLEKYNGQTVDQLIAMESDYRVDSLVLAFEQAMEQKKAKQKL
                     SPAEIDILAVEAMEREVNNGGYHQFFLNSSREYAAVLPSSLERIGCPVAAKIASDALA
                     CLKISGEVTAAKIDAALQRLGGTAVEDLGKMDERYFQNEEPIAGRLFARIKAQKGQIV
                     LR"
     gene            complement(2563..3513)
                     /locus_tag="VIS74_04090"
     CDS             complement(2563..3513)
                     /locus_tag="VIS74_04090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792453.1"
                     /translation="MHDFSPPSHPAEIFLDTDMATDCDDAGAMAVLHALADAGEARIR
                     AVVVNNKDAASAGAVAALNAFYGRAGIPLGMSGGGDIGAPAGAFVQALAGDTARYGHT
                     PRRDAPSAVDIYRRTLAAVADGRAVIASIGHLNNLHALLLSPPDAHSPLAGGELVRRK
                     VAHLVVMGGDYPEGKEHNFFARGSHAVTAEAINRWPTPILFSGFTLGLAVRTGPGLLA
                     LPETHPVRRAYALHPSRPLENGRPSWDQTAVLAAVRGPACFWELSPAGVNHIAADGSN
                     QWQSDPLGSHAYLIEKKPPAEAAAEIEALMLGRTGTPANA"
     gene            complement(3574..3894)
                     /locus_tag="VIS74_04095"
     CDS             complement(3574..3894)
                     /locus_tag="VIS74_04095"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEY5792454.1"
                     /translation="MPKSGIQIISDLPGTGPELKKGDRVLLCYDIQLSRGDFLVQDQE
                     ADWTVGDRNFVAGFRYGLEGMRAGGSRKFKASPHLCYRDAEAPGIPRNAVLICNIKRV
                     EIKA"
     gene            complement(3956..4489)
                     /locus_tag="VIS74_04100"
     CDS             complement(3956..4489)
                     /locus_tag="VIS74_04100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792455.1"
                     /translation="MNTLRISLLSLLIISAIGMARGESFAITADSLSAAIPAIKTAGL
                     EWMFLPHKQPFDPKAPQLPFDSGRPLRGVFTPAQFAVTARALSSQNLAAKNILSTIVD
                     PAARLDIPSVDGTNSRTVSLSATSLPDNKILVRTYGSDLSIAEEAAIEPGQTIVLSTA
                     GETSDRVSIYFITIKKG"
     gene            complement(4528..5040)
                     /locus_tag="VIS74_04105"
     CDS             complement(4528..5040)
                     /locus_tag="VIS74_04105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792456.1"
                     /translation="MSLSANSEFLDRSSVPLPTERDFDPHQGDLDAQWAWKNFGGLSL
                     NQAYGKFLSRPEIYQEDFMFMGIRAFDYYFPVIDRYLREVSIGEDHLGDCEASILGCA
                     AAAQLGWTGAVLPERLLLEMDDLRRFVTDNVARYAATPKDRRRILRDWRRVANEMEQY
                     QTANKSGRRG"
     gene            complement(5138..5584)
                     /locus_tag="VIS74_04110"
     CDS             complement(5138..5584)
                     /locus_tag="VIS74_04110"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY5792457.1"
                     /translation="MRRTKLPLMHNVGIVVEDIDSAIEFFTELGLDLEGRAPVEGEWA
                     DGVTGLHDMRVEIAMMRTPDGHSRLELSRFLAPRVVADHRSAPVNALGYLRVMFTVED
                     IDDTLARLRKYGAEIVDKVVQYEDTYRLCYIRGPEGILIGLAQELG"
     gene            complement(5725..6108)
                     /locus_tag="VIS74_04115"
     CDS             complement(5725..6108)
                     /locus_tag="VIS74_04115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792458.1"
                     /translation="MRLLIFLGIVTAGWGYESIPAIWRQELHSTVLSDYLPGEYFIIS
                     SIYTGRLGKDASIRHMQEVKVTAFGKSFSATLKDKNDRLAEIFTVTLFEMHDPQRMRA
                     NYEHIVYNEGEEYLKFSGSTILVRP"
     gene            complement(6185..6382)
                     /locus_tag="VIS74_04120"
     CDS             complement(6185..6382)
                     /locus_tag="VIS74_04120"
                     /inference="COORDINATES: protein motif:HMM:NF027249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HEY5792459.1"
                     /translation="MIIIEPTATGFSAYSPDVPGCVSTGKTRTQVEKNMKEAIEFHLD
                     GLREEGFEIPSPHSEPAFCEV"
     gene            complement(6391..6576)
                     /locus_tag="VIS74_04125"
     CDS             complement(6391..6576)
                     /locus_tag="VIS74_04125"
                     /inference="COORDINATES: protein motif:HMM:NF019540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HEY5792460.1"
                     /translation="MKVRDILKLVQNDGWYLVRTKGSHRQFKHSSKPGLVTIPGHPKD
                     DLAPGTQNSILKQAGLK"
BASE COUNT         1569 a         2176 c         2204 g         1538 t
ORIGIN      
        1 ccgcctcctg gccatgatcc gaaaaaatcc agacatggta atcccgccgc cgggacgcgt
       61 gcgccgcccg gtggagatcc cggatcgaac ggtcgatccc gagcaggctc caatgggaaa
      121 aacggccggc gggaccgcgg cgatgggcca tttcatcata tcccacgaaa ttcagatgga
      181 tcaccggcag tccgcgggcg agatccacct tgccgccgac cgtgatcacc tcgcggaggg
      241 caatgccggc gcacattcgg gaaaggacgg tggccatttc gggcaggggg gagtatccgc
      301 ggcacatgcc ggccagcgcg tcggtgagcc caatggcaaa ttccagcaat accagcgcga
      361 tcacgcggag caccgagatg ggatgcaata tcaggacggt gaagagcgtc agcagatggc
      421 ttctgcgcca gatgtcgccg attcccattc gagaaatgca gaaatgggtt tccccgctgg
      481 cggcgccgcc ggaaaagatg tttgaccatg agcttccgcc cgtgagcagg ccctggccgt
      541 gccgggcaaa ttgccgttcg agctgccggg ccagttccgg attaaacagg ctggtgattt
      601 ctcctgaatc gggatcccgg aagctgaagg ccggaactcc gccgcgaacg ccaaaataca
      661 gctccgcctg cacggcgggc gtggtgctgg gcagcccgct gtagaaagtc agcagctcgt
      721 gctcctcccg ccggcaaagt gatttcagaa aggggagccg gccttcgtgg atggcttttt
      781 ccaattgccg gcgggaaagg ccatccacct ggatcaatag cagccccggc tcttcttcgg
      841 tgcctttgct gaccggcagc cgcagttggc ggataatcca ttcactccgg ttaaacaact
      901 gccgccagga ccccacccac gcttcgattt ccgcgaacat ggctcattcc acccgccagc
      961 cgggatgatc gcgcagcaaa tgggcttttt gctgatgaaa atcgcagagg cccatcccct
     1021 tcaccggata ttccctgacg ggctggtccg gataatggat cgccattttc cacggcattc
     1081 cgtgccggtc gtgaaacacg atccggcaat gccccggagg agcttcctcc gccttttgca
     1141 tcaaggattt gctcatgcct catcggtggg ggtttcccgg agagcggatg ggcgcaggct
     1201 cagacccgga gccgggcgaa ggggatcgtc cggcatccga tccgtttccg ccgccgccga
     1261 gggccacggc aaggccccat cggaggaatg cggaaatccg tttcattctc cttgggatcg
     1321 tttggccaag gggaagcggc tgggtcgaaa ctcaatgacg gcccggcggc cagtctggtc
     1381 cgaccttatt tctccgcctc gaccgccgag gcaggcactc ggcggatgac cacaaatgcc
     1441 ttccggtctt tgttgagata ggccgttccc tcgaaaaaat agcccggagt tgccggatag
     1501 gaaaatttct tcggcccttg atccgccggc acggtaatcg atcccgcact accaatcgtc
     1561 cccacaaatc ccggccccac ctggtaggga ccattagccc cttcttgaat gtaaatctcc
     1621 ccgcggagag acgattcctg ggaaagaacc gcctggcggg agaggctgta ttcgtttgcc
     1681 ggtctcgaac atcccaaaag acagacccct gccatgaaca ataacccacg cggaatcatc
     1741 ttattgccaa acgcccggaa ccgggcgacg cgggcggcct gcccgcattc acttcagtga
     1801 ttttgcccgg cgctttcgtc atcttagaac gatctgacct ttctgcgcct tgatgcgggc
     1861 aaacaatctg ccggcaatcg gctcttcgtt ttggaaatag cgttcgtcca tctttccgag
     1921 atcctccacc gccgtgccgc cgagccgctg caaggcggca tcgatcttcg cggccgtcac
     1981 ctctccgctg atcttcaagc aagcaagcgc atccgaagcg atcttcgcgg cgaccgggca
     2041 gccgatccgc tcaagcgacg aaggaagaac cgcggcatac tcccttgaag agttcagaaa
     2101 gaactgatga tacccgccgt tattcacttc gcgctccatc gcctcgacgg cgaggatatc
     2161 aatctcggcc ggcgagagct tttgcttcgc tttcttttgt tccatcgcct gctcaaacgc
     2221 cagaaccagc gaatcgacgc gataatccga ctccatcgcg ataagctgat cgacggtctg
     2281 accgttgtat ttttccagga atttcatttc tttggcttcg cttctcatga cgatgcctcc
     2341 gacgaggagc agagcgatga catgacgcat aggggttttc ccgaaaagta gagtagatgg
     2401 aaattcgtgc aatcttggag aatgggggat catgtttccg cgtgagaaat tgcgggcggc
     2461 gggggtagcg ctccatttgg ctaggatcac cccgatccgg aggccgggct ccggtggtgg
     2521 aaggatgaat gcgcgaagcg gccacggtgt cactcgcgtg cgtcacgcgt ttgccggcgt
     2581 tcccgtgcgg ccgagcatca gagcctcgat ctcggcggct gcctcggcgg gcggtttttt
     2641 ttcgatcaaa taagcatggg aaccaagcgg gtcggactgc cattggttgg agccgtcggc
     2701 cgcgatgtgg ttgacgcccg ccggactgag ctcccagaag caggcggggc cgcgcacggc
     2761 ggcgagcacc gccgtctggt cccagctggg gcgtccgttt tcaagcggcc ggctcgggtg
     2821 aagggcatag gcgcggcgga cgggatgggt ttcgggcagg gcgaggaggc cggggccggt
     2881 ccgcaccgcg agcccgagcg tgaagcccga aaaaagaatg ggcgtgggcc agcgattgat
     2941 ggcctcggcg gtgaccgcgt gcgagccccg cgcgaagaaa ttatgttcct tcccttccgg
     3001 gtagtcgccg cccatcacga cgagatgggc caccttccgg cgcacgagtt ccccgccggc
     3061 cagggggctg tgcgcatcgg gcggcgagag cagcagggcg tgcagattgt tgagatggcc
     3121 gatgctggcg atcaccgccc ggccgtcggc aacggccgcg agggtgcggc ggtaaatgtc
     3181 cactgcggag ggcgcgtccc ggcgcggggt gtgcccgtag cgggcggtat cgccggcaag
     3241 cgcctgcacg aaggcgccag cgggcgcgcc gatgtcgccg cctccagaca tgccgagggg
     3301 gatgccagca cgcccataga aggcattgag cgcggccacg gcgccggccg aggcggcatc
     3361 cttattattt acgacgacgg cgcggatgcg ggcctctccg gcatcggcga gggcgtgcag
     3421 cacggccatc gcgccggcat cgtcgcagtc ggtcgccatg tcggtgtcga ggaaaatctc
     3481 agccggatgc gagggagggg agaagtcgtg catgggttca ttcggacgac ggccaatgat
     3541 cgcgattttg ccgcgatccg tcaaacggcg cggtcaggct tttatttcca ctcgtttgat
     3601 gttgcagatg aggacggcgt tcctggggat tccgggcgcc tccgcatccc ggtagcacaa
     3661 atgcgggctg gccttaaact tgcgcgagcc gcccgcccgc atgccctcca acccataccg
     3721 aaagccggcg acaaagttcc tgtctcccac ggtccaatcg gcttcctggt cttggacgag
     3781 gaaatcaccg cgggagagtt gaatgtcgta gcaaaggaga acccggtccc ccttctttaa
     3841 ttcgggtccg gttccgggca ggtcggaaat gatttgaatg ccggattttg gcatgattgc
     3901 tggaattccc ttccaccgta aatcggcgca ttttgatccg caattcagga tcttgttagc
     3961 cctttttgat cgtaataaaa tagatgctta ccctatcgct tgtctctccg gcggtagaga
     4021 gaacgatcgt ctggccaggt tcgatggctg cttcctcggc tatggaaaga tcgcttccgt
     4081 aggttctgac taatatcttg ttgtccggaa gcgaggttgc ggagagggaa acagtccgcg
     4141 agtttgtccc atcaactgaa gggatatcca atcgggcagc tggatcgacg atggtggaaa
     4201 gaatattctt cgccgcaaga ttctgactgg agagagcccg tgccgtcacc gcaaactgcg
     4261 cgggcgtaaa tacgccacgc agcggccgtc ctgagtcgaa tggcaattga ggggctttgg
     4321 gatcgaaggg ctgcttgtgg ggcaaaaaca tccattcgag gccggcggtt ttaatggctg
     4381 ggatcgcggc actcagcgag tcagcggtaa tggcaaaact ctccccgcgc gccatgccga
     4441 tggcagaaat aatgagcaag gaaagcaatg atattctgag ggtgttcatg gcgggttaga
     4501 gttgaaattc taggctaacg tccgtggtca gccacgcctg cccgacttat tcgcggtttg
     4561 gtattgttcc atttcattgg caaccctccg ccaatcccgc aatattcttc gcctgtcttt
     4621 cggggtagcg gcatatcgag ccacattgtc ggtgacgaat cgccgcaagt catccatctc
     4681 cagcagcagc ctttctggta aaacggcccc agtccaacca agctgggcgg cggcggcgca
     4741 tcccagtatg gaagcctcac agtcaccaag gtgatcttcc cctatggaga cctccctgag
     4801 gtagcggtcg atgaccggga aataataatc gaatgcccga atgcccatga acatgaaatc
     4861 ctcctgataa atctcgggtc gcgaaagaaa cttcccatag gcctgattca aggataggcc
     4921 gccgaagttc ttccaagccc actgtgcatc caaatccccc tggtgcgggt caaaatcccg
     4981 ttccgtgggc aaagggacag aagaccgatc caagaactca gagtttgcgg acaggctcat
     5041 ttatttagcg aatgttcaag gtgaggcacg gagcgcagcg gagttgcctc caccgccttg
     5101 ttcggccttt gattcatcgt gatgatactt gagtgcgtta cccgagctct tgcgcgagcc
     5161 caatcaaaat tccctcgggg ccacggatgt agcaaagtcg atacgtgtct tcatactgga
     5221 ccactttgtc gacgatctcc gcgccgtatt tgcggagacg cgcgagcgta tcgtcgatgt
     5281 cctctacggt gaacatgacc cgtaggtatc caagagcgtt cactggggca ctacggtgat
     5341 cggcgaccac acgcggagcg aggaatcggg acagttcaag ccgactgtga ccgtccggag
     5401 ttcgcatcat ggcaatctcg acgcgcatat cgtgcaatcc cgtgacgccg tctgcccatt
     5461 ctccttcgac tggggcgcga ccctcgagat caaggccaag ttcggtgaag aactcaatgg
     5521 cggagtcaat gtcttctacc acaatgccca cgttgtgcat tagcggcaat ttggttcttc
     5581 tcatgatcta tagatttcct ttaggtgccg aacgtcggcc atcactcgcg accggacgcg
     5641 gcgcacttcg tgcggagcgt agcgaccagc acgaagtgtg acgcggtagg gagttgagtg
     5701 gatggcattg ttcggactct tttatcatgg tcgcacgaga atagttgaac ccgaaaactt
     5761 caaatattct tcgccttcat tgtagacgat gtgttcgtaa tttgcccgca tcctttgcgg
     5821 gtcatgcatc tcgaaaagcg taactgtgaa tatttccgcc aatcgatcat ttttgtcttt
     5881 taaggtcgct gaaaagctct ttccgaacgc ggttactttg acctcctgca tatgccttat
     5941 cgaggcgtcc ttacccaaac ggcccgtata aatcgaagaa atgataaaat actctccggg
     6001 taaataatcc gacaaaacgg ttgaatgcaa ctcttgacgc cagatcgccg gaatggactc
     6061 ataaccccat ccggcagtga cgatgcctag aaagatgagc aagcgcaaag agttcatttc
     6121 aattccgaac gtccgccgtc agccacgcgc tttagcgcgt tggctggacg gcatggttag
     6181 ctctttatac ttcgcagaag gctggttcgg agtgaggcga aggaatctca aatccttctt
     6241 ctcggaggcc atccaagtga aactcaatgg cttctttcat gtttttctca acttgagtcc
     6301 gtgttttgcc tgtggaaaca cagccgggca catccggtga gtaggcggaa aaaccagtgg
     6361 cggtcggctc aatgatgatc aaatatcggc tcattttaaa cctgcttgct tcaagatact
     6421 gttttgggtt ccgggagcaa gatcatcttt tgggtgtccg gggatggtga ccaatccagg
     6481 tttggaggaa tgcttaaact ggcgatgact gcccttcgtg cggacaagat accatccatc
     6541 gttttgcacc agcttgagaa tgtccctgac cttcactgag atatgtcatc atctcgtggg
     6601 aaatgcttca aggttatttc gctaacgacc caagctcagc gacccggcgc acgggacgcg
     6661 tggattgcac ccgcagcgcg atggccgggt tcgctgatgg ttaggcgatt tgattgctca
     6721 ttgggacttc agaataggaa gacggacccg ccctcagggc gaggcgggag gttcggactg
     6781 ccacgcccgg tgaagcaact ccaagaagga cggtgcctca ggcaagggca cagatccaat
     6841 ccggtggacc gcgatacccg gtgtccatga gttcccacag ccccggccaa cgccgacagg
     6901 tcgccaagcg tgacctcttg gtcgcgctgg aggataccga agcgatccaa ctgcctgcgg
     6961 acaataccca tcgtggtccc gcccaacata tcggccacgg gccacaccac cgcagtgccg
     7021 tcccaaaagg ccagattcca gatcgacccc tcgctgagcc gaccccgacg gtccacgaag
     7081 gcggcgtcgt caaacccatg tccaacggcc tgacgcagga aatacgtctt agccacctcg
     7141 ccaacatgct tcacggccgg gagcaccctt tcgtaatcga tcgtcgccag cgccagcggg
     7201 ccggccggac cgttcgaggg cggtccagtg cggaccagca cttggggctt cacctcaggt
     7261 ccggccacgg tgaactcacc tgtcggcgag aacaccgtgg ccatcagcga gaggtcagcc
     7321 ggaccggact ccagcgccgc ccgaagatag agcctgtccg agaagccgag atttcagaga
     7381 gtggcaagat ttggtcaatt ttccggattt tgtcggctca aaaacaccac aacatataga
     7441 tatttcggat gcgccctgcg cgcgggatga gcgattttca acgactt
//
LOCUS       DATDAY010000209         2133 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_2043046, whole genome shotgun sequence.
ACCESSION   DATDAY010000209 DATDAY010000000
VERSION     DATDAY010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2133
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2043046"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..616)
                     /locus_tag="VIS74_04130"
     CDS             complement(<1..616)
                     /locus_tag="VIS74_04130"
                     /inference="COORDINATES: protein motif:HMM:NF017771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF899 domain-containing protein"
                     /protein_id="HEY5792461.1"
                     /translation="MNTTTPALPKIVSRAEWAAARAAFLLKEKQATRARDALNAERRR
                     LPMVEIEKDYLFEGPGGTACLPDLFEGRRQLILYHFMFDPDWDEGCPGCSMVVDNMGH
                     PAHLHARGISRVLVSRAPLAKIEPFKKRMGWAEPWFSSFGSDFNRDFGVTTDEGEIFG
                     LSVFLRDGDQVFQTYSTNGRGVEYLGSSFSYYDLAPFGRQEVWED"
     gene            complement(726..2042)
                     /locus_tag="VIS74_04135"
     CDS             complement(726..2042)
                     /locus_tag="VIS74_04135"
                     /inference="COORDINATES: protein motif:HMM:NF042530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6596 domain-containing protein"
                     /protein_id="HEY5792462.1"
                     /translation="MSGATPDAPNGGEPAAAQVVEHFFRHETGRLHATLTRVFGFENL
                     ALIEDVAQGALLQALRTWSIGGVPANPSAWITRVALNLARDALRHRRMAFAKEANLIT
                     HLEQMLPDAAASENGGPEIADDTLRLMFVCCHPLLSPDAQAVLALKVMCGFSTAEIAQ
                     AFLSSEAAIEKQLTRTKQRLREAQVGFEIPAGAELSARLDGVLAALYLLFNEGYKAST
                     GERLLREELCQEAVRLMTLLLAHPAGQKPRSHALLALMLLTAARFPSRLDEHGELLRL
                     EDQDRSKWSQPLIERGLIHLTAAARGNELSEYHLQAGIAACHCVAPDYASTDWARILR
                     HYDELNRRKPSPILALNRAVAVAQLRGPRAGLDAVAAIPRRERLESHYLLHAVTGELH
                     WRLKNDRAAAESFRRALHLVHVGPEQTHLTRMLNRVTGEPDGRKKD"
BASE COUNT          371 a          655 c          736 g          371 t
ORIGIN      
        1 aatcttccca gacttcctgg cggccgaacg gcgccagatc ataatagctg aagctgctgc
       61 cgagatattc gacgccccgg ccattggtgg agtaggtttg aaaaacctgg tcgccatcgc
      121 gaagaaagac gctcaggccg aagatctcgc cctcgtccgt ggtaacgcca aaatcgcggt
      181 tgaagtcgct gccgaacgag gagaaccacg gctcggccca gcccatccgt tttttgaacg
      241 gctcaatctt ggccagcggg gcgcgggaca cgagaacccg cgagatgccg cgcgcgtgca
      301 ggtgggccgg atggcccatg ttatccacga ccatggaaca accggggcag ccctcgtccc
      361 agtccggatc gaacatgaaa tgatagagga tgagctggcg gcgcccctcg aacaaatcgg
      421 gcaggcaggc cgttcctccc ggaccttcga agaggtagtc cttttcgatt tcgaccatgg
      481 gcagacggcg gcgttcggcg ttcaacgcgt cgcgcgcccg ggtggcctgc ttttccttga
      541 ggagaaacgc cgcgcgcgcg gcggcccatt cggcgcggga cacgattttt gggagagcgg
      601 gggtggtcgt attcatagat agaattcggc gggttcaggg gttttgagcg tggaataaat
      661 gaatgcggtc aaacatgtcc ataacatacc aactagtttg tatgttatgg cgaacgcgga
      721 attcgtcaat ctttttttct tccatccggc tctccggtca cccggttcag catccgggtg
      781 agatgggttt gctcgggccc gacgtgaacg aggtgaagcg cccggcggaa gctttccgcg
      841 gcggcgcggt catttttgag ccgccaatgc agctcgccgg tcacggcgtg gagcaggtaa
      901 tgcgactcca gccgttcgcg ccgcgggatg gcggccaccg cgtccagccc ggcccgcggt
      961 ccgcgcagtt gcgccacggc caccgcgcga ttcagggcca ggatgggcga gggtttgcgg
     1021 cgattgagct catcgtagtg gcgcaggatg cgcgcccagt ccgtggaggc gtaatccggc
     1081 gcgacgcagt ggcaggcggc gatgccggcc tggaggtgat attcgctcag ctcattcccc
     1141 cgggccgccg cggtcaggtg aatcagcccg cgttcgatga gcggctggct ccatttcgag
     1201 cggtcctgat cctccagccg cagcagttcg ccgtgctcat cgaggcggga ggggaagcgg
     1261 gccgcggtca gcagcatgag cgcgagcagc gcgtggctgc ggggtttttg cccggcggga
     1321 tgcgcgagga gcagcgtcat caggcgaacc gcttcctggc acagctcctc gcgcagcagg
     1381 cgctcgcccg tggaggcctt gtagccttcg ttgaagagca gatacagcgc ggcgagcacg
     1441 ccatccagcc gcgcggacaa ctcggcgccg gccggaattt cgaagccgac ctgggcctcg
     1501 cgcagccgct gtttggtgcg cgtgagctgc ttttcaatgg ccgcctcgct gctcaggaac
     1561 gcctgggcga tctcggcggt gctgaacccg cacatcacct tgagcgcgag gacggcctgc
     1621 gcgtccgggg agagcagggg atggcagcag acaaacatca aacgcagcgt gtcgtccgcg
     1681 atttcaggcc cgccattttc ggacgccgcg gcatccggca gcatttgttc gagatgcgta
     1741 atgaggttcg cctccttggc gaaggccatg cgccggtggc gcaacgcgtc ccgggcgaga
     1801 ttgagggcga cgcgcgtgat ccaggccgac ggattggccg gcacgccgcc gatcgaccag
     1861 gtgcgcagcg cctgcaggag cgcgccctgg gccacgtcct cgatgagggc caggttttcg
     1921 aagccgaaca cccgcgtcag cgtggcgtgc agccggccgg tttcatgacg gaaaaaatgt
     1981 tcgacgacct gcgccgccgc cggttcgccg ccgttgggcg cgtctggagt ggctccagac
     2041 atcgcggttc aagcgtgggc gggctccgcc gccggtggtg aacacaaatt ggggcaggcg
     2101 ccggccacgg ggcgcacttc cacgacgagg ccg
//
LOCUS       DATDAY010000210         4666 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_2321269, whole genome shotgun sequence.
ACCESSION   DATDAY010000210 DATDAY010000000
VERSION     DATDAY010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 4666)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4666)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4666
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2321269"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..253)
                     /locus_tag="VIS74_04140"
     CDS             complement(<1..253)
                     /locus_tag="VIS74_04140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792463.1"
                     /translation="MTAIPTLVPALDAAAIEQAIQRMAREFISRHSDFAKWVVAGIPT
                     RGVEVARRLVAEIQKERGVAPDLGAVDISMHRDDLATRLR"
     gene            complement(326..559)
                     /locus_tag="VIS74_04145"
     CDS             complement(326..559)
                     /locus_tag="VIS74_04145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792464.1"
                     /translation="MSATKQETNGDWVKGSSLYQEFLAEREEILRHKWIESEKQGQDI
                     GFEKAFLDWILRHRSGWKEQRIKPLIAVPRPAA"
     gene            complement(678..1715)
                     /locus_tag="VIS74_04150"
     CDS             complement(678..1715)
                     /locus_tag="VIS74_04150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140663 - ATP-dependent FeS chaperone
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HEY5792465.1"
                     /translation="MTPEILRAALAQVKYPGFSRDIVSFGLVKDIHVHGSDVEVHLTL
                     TTADEKIPAQIRADAQQALLAVPGVGSVDVKIDLQAPPQPTGAVGPARIEGVKHIIAV
                     ASGKGGVGKSTVAANLALALAQTGAAVGLCDCDLYGPSIALMFGGANERPTADDEDRI
                     VPIERYGLKLMSMGFLLDDASPAVLRGPMVTRYTQQFLRQVAWGRLDYLILDLPPGTG
                     DIQLTIVQTVALAGTVVVTTPQEVALIDARKAVTMFSKTSVPILGIVENMSYFLSPGD
                     GRRYDIFGSGGGGREAARLRVPLLGQVPIEMEIRESGDTGRPAVDKFPRSATAQIFRE
                     IAQKVRQRVEK"
     gene            complement(1715..2935)
                     /locus_tag="VIS74_04155"
     CDS             complement(1715..2935)
                     /locus_tag="VIS74_04155"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HEY5792466.1"
                     /translation="MKIVVAYSGGLDTSVLVKWLKENYSAEIIAFCADVGQEEELDGL
                     EEKALKTGASKCYIDDLREEFAKDFIFPMMQAGALYEGQYFLGTSIARPLIAKRMVEI
                     AAAEGAAAVAHGATGKGNDQVRFELTAAALAPDLEVIAPWRQERFRDQFPGRSEMIAY
                     AEANGIPIAATAKKSYSMDRNLLHISFESGMLEDPWFDASAPEQKDMYKLSVSPEDAP
                     DAPEYAELDFEKGLCVAVNGRALSPLGVMLELNRLGGKHGIGRVDMVENRFVGMKSRG
                     VYETPGGAVLHFAHRQMESITQDREVMHLRDSLIPKYSTLVYNGFWFAPEREALQALV
                     AESQKNVTGTVRVKLYKGNLIAAGRKSPLSLYNPDIATMEADPTQAYNQSDATGFIRL
                     NALRLKVAAKVEKK"
     gene            complement(2993..3430)
                     /locus_tag="VIS74_04160"
     CDS             complement(2993..3430)
                     /locus_tag="VIS74_04160"
                     /inference="COORDINATES: protein motif:HMM:TIGR00028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system VapC family ribonuclease toxin"
                     /protein_id="HEY5792467.1"
                     /translation="MTRLLDINVLMALFDPRHEFHDAAHRWFSATKADGWASCPLTIN
                     GFVRILSHPAHPAALGTVEETAAKLAEFSGATPHSFWPDTLSLHGSRGFRLGLIPSSR
                     HLTDIYLLGMAVHRKGIFATFDRRVPVGAVVGGAEAIEIIPEV"
     gene            complement(3432..3683)
                     /locus_tag="VIS74_04165"
     CDS             complement(3432..3683)
                     /locus_tag="VIS74_04165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792468.1"
                     /translation="MKTTIDIPEALYRKAKIRAARTGSTLRDLVVGALEGSLMEVPSK
                     PAPKGGRKKTRNGVPLLPAGDRPVVTNEFINQLRDELGI"
     gene            3728..>4666
                     /locus_tag="VIS74_04170"
     CDS             3728..>4666
                     /locus_tag="VIS74_04170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792469.1"
                     /translation="MKLSPVFGQPSWRLKSDSVQLALTQTGGHLGPVTFKLGGKFVQP
                     FHVAPWHGEKRTLAGLPPILQALRGDFFCMPFGGNEKPYGRERHPVHGETANREWTLE
                     SATDTSLRASLRTEVRSGRVLKEIFLRPGQTALYVRHQLEGFRGPMSFGHHAMLKFAT
                     EAHLSTSPFVYGQVFPGQFENPARGGYSSLRAGVVFKSLRRVPLAAGGFADLSRYPAR
                     EGFEDLVMVCAGPRQPLAWTAAAFPREGFVWFALKDPRVLASTVFWHSNGGRHYAPWN
                     GRHRGVLGLEEVTSYFHTGLAESAAPNPVSARGIPTS"
BASE COUNT          873 a         1514 c         1341 g          938 t
ORIGIN      
        1 gccgcaaacg ggtggccaga tcatcgcgat gcatcgaaat atccacggcc cccaaatccg
       61 gagccacgcc ccgctccttt tgaatttccg ccaccagccg ccgggccacc tcgaccccgc
      121 gggtcggaat tcccgccacg acccatttgg caaaatcgga atggcgcgaa atgaattccc
      181 gggccattcg ctgaatcgcc tgctcgatgg cagccgcatc cagggcgggg acaagtgtgg
      241 gaatcgcggt catgaacggc agccgttttc gctcaccgga tgcggagaga aaagcggaaa
      301 atacaaaatg cgggccgcga aggcgttaag cggccgggcg cggcaccgct atcagcggtt
      361 tgattcgctg ctccttccat ccggaccgat gcctaaggat ccagtcaagg aaggcttttt
      421 caaaaccgat gtcctgcccc tgcttttcac tttcgatcca tttgtggcgc aaaatctcct
      481 cccgttcggc gagaaattcc tgatacagcg atgatccctt gacccagtcg ccgttggttt
      541 cctgcttagt cgcactcata aatttaatga cctaagggaa gtggggactt ttcatttcca
      601 caagtcaatc ggattttttc agccccctcc atctacttag ctgggttaat aatattctaa
      661 agataaaaac ggagccctca cttctccacg cgctgccgca ccttttgcgc gatttcccgg
      721 aaaatttgcg cggtcgcgct ccggggaaac ttgtccaccg cggggcggcc ggtgtcgccg
      781 gattcgcgga tttccatttc gatcggcacc tgcccgagca gcggcacccg gagccgggcc
      841 gcctcgcgcc cgcccccgcc gctgccgaaa atgtcgtagc gccggccatc gccgggactg
      901 aggaagtaac tcatgttttc cacgattccc aaaatcggga cgctcgtctt ggagaacatc
      961 gtcacggcct tgcgggcatc aatgagcgcc acctcctgcg gcgtggtcac caccaccgtt
     1021 ccggccagcg ccacggtctg aacgatggtc aattgaatgt cgcccgtgcc gggaggcagg
     1081 tcgagaatca ggtaatcgag ccggccccag gcgacctgcc ggaggaattg ctgcgtgtaa
     1141 cgcgtcacca tcggcccgcg cagcaccgcc ggggaggcat cgtcgagcag gaagcccatg
     1201 gacatgagct tcaagccgta acgctcgatc ggcacgatgc gatcctcgtc gtcggcggtc
     1261 gggcgctcgt tggctccgcc gaacatgagg gcaatgctcg gcccgtagag gtcgcaatcg
     1321 caaagcccca ccgccgcgcc ggtctgcgcc agcgccagcg cgaggttggc ggccaccgtg
     1381 cttttcccca ccccgccctt gccgctggcc acggcaatga tgtgcttcac gccttcaatc
     1441 cgcgccggtc ccaccgctcc ggtgggttgc ggcggcgcct gcaagtcgat tttcacatcc
     1501 acgctgccga ccccgggaac cgcgagcagc gcttgctggg cgtccgcccg aatctgcgcc
     1561 gggatttttt cgtccgccgt cgtcaaggtg agatgaacct ccacgtcgct tccatggacg
     1621 tgaatgtcct tcaccaagcc aaaggaaacg atgtcccggc tgaagccggg atacttcacc
     1681 tgcgcgaggg ccgcgcggag gatttcgggt gtcattattt cttttcaacc ttcgccgcga
     1741 ccttgagccg cagggcgttg agccggatga agccggtggc gtcggattga ttgtaggcct
     1801 gggtcggatc ggcttccatc gtggcgatgt ccggattgta caggctcagc gggctcttgc
     1861 ggccggcggc gatgagattg cccttgtaaa gtttgacgcg caccgtgccg gtcacgttct
     1921 tctgggattc cgccaccagc gcctgcagcg cctcccgttc gggagcaaac caaaatccgt
     1981 tgtaaaccag cgtgctgtat ttcgggatca acgaatcgcg caggtgcatg acctcgcggt
     2041 cctgcgtgat ggattccatc tgccggtggg cgaaatgcag gacggcgccg cccggcgttt
     2101 cgtaaacccc gcgcgacttc atgccgacaa agcggttctc caccatgtcc acgcggccga
     2161 tgccgtgctt gccgccgagg cggttgagtt cgagcatcac gccgagcggg ctgagcgccc
     2221 ggccgttgac cgccacgcag aggccttttt caaagtcgag ttcggcgtat tccggcgcat
     2281 ccggcgcgtc ctcggggctg acgctgagtt tatacatgtc cttctgctcc ggcgcgctgg
     2341 catcaaacca ggggtcttcc agcatgccgc tctcgaagct gatgtggagc aggttgcggt
     2401 ccatggaata cgacttcttt gccgtcgccg cgatcggaat gccattcgcc tccgcgtagg
     2461 cgatcatttc cgaccggccg gggaattgat cgcggaaccg ctcctgccgc cagggcgcga
     2521 tgacttccag atcgggggcc aatgcggccg ccgtcagctc gaagcgcacc tggtcattgc
     2581 ccttgccggt cgcgccgtgc gccaccgccg ccgcgccttc cgccgccgcg atttccacca
     2641 ttcgcttggc gatcaacggg cgcgcgatgc tcgtgccgag gaaatattgc ccctcgtaaa
     2701 gcgcgccggc ctgcatcatt ggaaaaatga agtccttcgc aaattcctcg cgcaggtcgt
     2761 cgatgtaaca cttcgacgcg cccgtcttga gggctttctc ttccagcccg tccagttcct
     2821 cctcctggcc gacatccgcg cagaaggcga tgatttccgc cgaataattt tccttcagcc
     2881 atttgaccag aacggaggtg tcgagccctc ccgaatatgc aaccacgatt ttcatgcacc
     2941 gaccgtttta catggccaac cgcgggattg agaagcccgc ttcggaaaaa tttcagacct
     3001 cgggaataat ttcaatcgct tcggctccgc ccactaccgc cccgaccgga actcggcgat
     3061 cgaaagtcgc gaaaatgcct ttacggtgaa ccgccatccc caacaaataa atatccgtca
     3121 aatgacgtga agacggaatc agccccaagc ggaagccacg cgagccatgc agcgaaaggg
     3181 tgtccggcca aaacgaatgc ggggtcgcac cggaaaactc cgccaattta gccgccgtct
     3241 cttccaccgt ccccagggcc gccggatggg ccggatgact gagaattcgg acaaacccat
     3301 taatggtcag cgggcaactt gcccatccgt cggccttggt cgctgaaaac cagcggtgcg
     3361 cggcatcgtg aaattcatga cgcggatcga acaatgccat caacacatta atgtcaagca
     3421 acctcgtcat ctcaaatgcc caattcgtct ctcaattgat taatgaactc attggtgacg
     3481 acaggccgat cccccgccgg caacagggga accccattgc gagttttttt tcggcctcct
     3541 ttgggagcgg gcttcgaagg aacctccatt aaacttccct ccagcgcccc caccaccaag
     3601 tcccgcaggg tcgaacccgt ccgcgcggcg cggatcttgg ctttgcgata cagcgcctcg
     3661 ggaatgtcaa tggtcgtctt catgcttcca tattgatata aatacggaaa tccgtcaatt
     3721 ttcctccatg aagctctccc ccgtcttcgg ccagccttcc tggcggttga aatccgattc
     3781 ggtgcagctc gcattgacgc aaacgggcgg ccatctggga ccggtcacct ttaagctcgg
     3841 cggaaaattc gttcagccgt ttcacgtggc tccctggcac ggtgagaaac ggacgctcgc
     3901 cggcctgccg cccatcctcc aggcacttcg tggcgacttt ttctgcatgc cattcggggg
     3961 aaatgaaaag ccttatgggc gggaaagaca tccggttcac ggggaaacgg ccaaccggga
     4021 atggacgctt gaaagcgcca cggacacctc gcttcgcgcg agtttgcgaa ccgaggtgcg
     4081 gagcggacgg gttctcaagg aaatcttcct ccgccccggc cagaccgcgc tctacgtgcg
     4141 ccatcaactc gaaggtttcc gcgggccaat gagcttcggg catcacgcca tgctgaaatt
     4201 tgccaccgag gcgcaccttt ccaccagccc gttcgtttac ggacaggtct ttcccgggca
     4261 gttcgaaaat cccgcgcggg gcggttattc gtcgctccgc gccggggtgg tctttaaatc
     4321 gctgcgccgt gtgccgctcg ccgccggcgg atttgccgat ctttcgcgtt atcccgcccg
     4381 cgagggcttc gaggatctcg tgatggtctg cgccggtccg cgccagccgc tggcctggac
     4441 cgcggcggcg tttccgcggg aaggcttcgt gtggtttgcc ctcaaggatc cgcgcgtgct
     4501 ggcttcgacg gtcttctggc actcgaacgg cggccgccat tacgccccgt ggaacggccg
     4561 ccaccgcggg gtgctcgggc ttgaggaagt caccagttat tttcacaccg gcctcgcgga
     4621 aagcgcggcc cccaatccgg tgtccgcgcg ggggattccg acgagc
//
LOCUS       DATDAY010000211         4229 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_1535571, whole genome shotgun sequence.
ACCESSION   DATDAY010000211 DATDAY010000000
VERSION     DATDAY010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 4229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4229
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1535571"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..681)
                     /locus_tag="VIS74_04175"
     CDS             complement(<1..681)
                     /locus_tag="VIS74_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006983460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylenetetrahydrofolate
                     dehydrogenase/5,10-methenyltetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HEY5792470.1"
                     /translation="MKRIDGKAISEKFNAQTQARAEELRARGLTPGLAVVLVGQDPAS
                     VFYVRQKDKVSQQLGFYSRKIELPAETTQDELLLVVDELNRDPRVHGILVQSPPPPHI
                     DEAAIVLALDPRKDVDGFHPLNVAKLAMDDPSGFVPCTPLGCLKLLEESGIETSGKHA
                     VVLGRSMIVGKPMALLLMRKGKGGDATVTVCHSRTRDIAAITSQADILIAAIGRAEYV
                     KPEHVKEGA"
     gene            complement(668..1303)
                     /locus_tag="VIS74_04180"
     CDS             complement(668..1303)
                     /locus_tag="VIS74_04180"
                     /inference="COORDINATES: protein motif:HMM:NF015961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEY5792471.1"
                     /translation="MSREKWIAALAVGILRLLFGTLRVKIEDRSGFTRKSPDFPVIVT
                     FWHNRILAITTAFLEVYPNHLRKGVSVLTSPSRDGEILSQIMAGFRMGSIRGSSNKRA
                     SAAAKECIRRLESGADLAVTPDGPRGPRYVMSPGLILMAQQTGARILPVHAHFSRCLK
                     MKTWDEFRIPLPFSTVHIVASPYETIPPTATEAEFEAQRKRIETILINEAD"
     gene            complement(1300..2313)
                     /locus_tag="VIS74_04185"
     CDS             complement(1300..2313)
                     /locus_tag="VIS74_04185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792472.1"
                     /translation="MDSALPHLDKLRVLLCQLGDTIRNSVIAARRSQTLEASAAVSHH
                     THADTIYAIDKVSESALVSWFESHWPADEPVEVVWEGVDETAPPCFPRGVPSEATKWK
                     CLIDPIDGTRGIMYDKRSAWALAGLAPQSGESTSLSDIFIAAMTEIPTTKQWRADQLS
                     VVEGQGIVAEAVNVLDGTRTPFTIQPSKATQFDHGFASFAKFFPEGRTLIAQIEEAIW
                     DELVGIGEHSSPTIFDDQYISTGGQFYEVLMGHDRLIADIRPLVYARLDLDSSLVCHP
                     YDACVWPILMEAGIVYEAPDGGFPDAPLDTTSPLAWILFANPQLAKLARPVIHRALET
                     LLQ"
     gene            complement(2455..2712)
                     /locus_tag="VIS74_04190"
     CDS             complement(2455..2712)
                     /locus_tag="VIS74_04190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792473.1"
                     /translation="MTSRTTARFWGCYRKLPAEVRELARKNYRLWREDVHHPSLHFKP
                     VSKGQWSVRIGIHYRATGRFSGSDEFTWDWIGTHGEYDRLA"
     gene            complement(2709..2933)
                     /locus_tag="VIS74_04195"
     CDS             complement(2709..2933)
                     /locus_tag="VIS74_04195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792474.1"
                     /translation="MSTVAEIKEAIAKLSPQEYCELMAELHPFPDDEWDKQMKADFAS
                     GKMDWLVKETEAVIREGKTVPLETVLAEDE"
     gene            complement(3122..3195)
                     /locus_tag="VIS74_04200"
     tRNA            complement(3122..3195)
                     /locus_tag="VIS74_04200"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3161..3163),aa:Gln,seq:ctg)
     gene            complement(3265..3690)
                     /locus_tag="VIS74_04205"
     CDS             complement(3265..3690)
                     /locus_tag="VIS74_04205"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEY5792475.1"
                     /translation="MNFRKTLVQQNPLLFNVTSLVDILMVLVVFLLMTWATTQVESDI
                     GIQLPTAKSGEARNISPTQITVNIRPDNTLTVNQKTVDDTAFTDMLGKLAKLNPDQLV
                     IVRADKTVSYERMLNVLDLCRQAGISDIGFGSVPEDKNK"
     gene            3786..>4229
                     /locus_tag="VIS74_04210"
     CDS             3786..>4229
                     /locus_tag="VIS74_04210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792476.1"
                     /translation="MPKFLFSLLLAVFFCSCAVTGSPIGQTQVRIKGYPVSLLQQKTG
                     EVFTRDSFMLVQSQPDRMVFERGGGNTEEVFYGDWMKRNTAVRVTVHFIRKGSNDYLL
                     RTDSRILRNPGSSFEDQSDLFDMQSLKYRRYLSEVKKELQRDFPAA"
BASE COUNT          806 a         1229 c         1356 g          838 t
ORIGIN      
        1 cgcgccttcc ttcacgtgtt cgggtttgac gtattccgcg cgtccgatgg cggcgatcaa
       61 aatgtccgcc tgcgaggtga tggcggcaat gtcccgggtg cgggaatggc agacggttac
      121 cgtggcgtcg ccgcccttgc ccttgcgcat gagcagcagg gccatcggct tgccgacgat
      181 catgctgcgg cccagcacga ccgcgtgctt gccgctggtc tcgatgccgg attcctccag
      241 cagcttgagg cagcccagcg gcgtgcaggg aacgaagccg gagggatcgt ccatggcgag
      301 cttggcgaca ttgaggggat gaaacccgtc cacgtccttg cgcggatcga gggcgaggac
      361 aatggccgcc tcatcgatgt gcggcggcgg cggggattga acgaggatgc cgtgaacccg
      421 cggatcgcgg ttgagttcgt ccaccacgag gagcagttcg tcctgcgtgg tttccgccgg
      481 cagttcgatc ttgcgggaat aaaagccgag ctgctgcgaa accttgtcct tttgccgcac
      541 gtaaaagacg gacgccggat cctgcccgac gagcacgacg gcgaggccgg gcgtcagacc
      601 ccgggccctg agctcctccg cccgcgcctg ggtttgcgcg ttgaattttt ccgagatggc
      661 tttgccgtca atccgcttca ttgatcagga tggtttcgat tcgtttgcgt tgtgcctcga
      721 attccgcctc cgtggcggtg ggcgggatcg tttcgtaggg gctggccaca atgtgcacgg
      781 tggaaaaggg aaggggaatg cgaaactcgt cccaggtttt cattttgagg cagcgggaga
      841 aatgggcgtg caccggcaga atccgcgcgc cggtctgctg cgccatgaga atgagccccg
      901 gactcatcac gtagcgcggc ccgcgcggcc cgtccggggt gaccgccaga tccgcgccgc
      961 tttcgagccg gcggatgcat tccttggccg ccgcggaagc ccgcttgttg ctggacccgc
     1021 ggatggagcc catccggaag cccgccatga tctgcgaaag gatttccccg tcccggctcg
     1081 ggctggtcaa tacgctgacg cccttgcgca ggtgattggg atagacctcc agaaaggcgg
     1141 tggtgatcgc gagaatgcga ttgtgccaga acgtgacgat caccggaaaa tccggggact
     1201 tccttgtgaa gccgctgcgg tcctcgatct tcacccgcag cgtgccaaag agcagccgga
     1261 ggattcccac ggcgagggcg gcgatccatt tctcccggct cattggagca gggtttccaa
     1321 ggcgcgatgg atcaccgggc gggccagttt ggcgagttgc gggttggcga acaaaatcca
     1381 ggccagcggg ctggtggtgt ccagcggcgc gtcggggaat ccgccatcgg gcgcttcgta
     1441 aacgatgccc gcctccatga gaatcggcca cacgcacgcg tcgtagggat ggcagaccag
     1501 cgaggaatcc aggtccagcc gcgcgtaaac gagcgggcgg atgtcggcga tcagccggtc
     1561 gtggcccatc agcacctcgt agaattgccc gccggtggag atgtattgat cgtcgaaaat
     1621 cgtgggcgag gaatgttcgc cgatgccgac gagttcatcc cagatcgctt cctcgatttg
     1681 cgcgatcaag gtgcggccct cggggaaaaa tttcgcgaac gacgcgaagc cgtggtcaaa
     1741 ctgcgtggcc ttcgagggct gaatcgtgaa gggggtgcgc gtgccgtcga gcacgttgac
     1801 ggcttcggcg acgatgccct ggccttcgac gacgctgagc tgatccgcgc gccattgctt
     1861 ggtcgtgggg atttccgtca tggcggcgat gaagatgtcg gaaagcgagg tggactcccc
     1921 ggattgcggc gccagcccgg ccagcgccca ggccgaacgt ttgtcataca tgatgccgcg
     1981 cgtgccgtcg atcggatcga tcaggcattt ccatttggtc gcctcgcttg gcaccccgcg
     2041 gggaaagcag ggcggcgcgg tttcgtccac gccctcccag acgacctcga ccggctcgtc
     2101 cgcgggccaa tgggactcaa accagctgac cagggcgctc tcgctgacct tgtcgatggc
     2161 gtaaatggtg tccgcgtggg tgtgatgcga gacggccgcc gaggcctcga gggtctggct
     2221 gcggcgggcg gcgatgacgg aattccggat cgtgtcgccc agttggcaga gcaggacgcg
     2281 gagtttgtcg agatgtggga gagcggaatc cattctttaa tgggaaaggt tgcgaaaagc
     2341 gcccgcccaa ggaaagcagg aaaaacgccc cggacccccc caatcgaaaa tcgaaactcc
     2401 aaaatcgaaa attcggcatg ggtgtcttgc atggattcgt tcccattccc gcaatcacgc
     2461 cagccggtca tattcgccat gcgtgccaat ccagtcccac gtgaattcat cgctgccgga
     2521 aaagcgcccg gtggcgcggt agtggatgcc gatacggaca gaccattgcc ccttgctcac
     2581 cggtttgaaa tgcaacgagg gatgatgaac gtcctcccgc caaaggcggt aattcttgcg
     2641 ggcaagttca cggacttcgg cggggagttt gcggtaacag ccccaaaacc gggccgtcgt
     2701 ccggctggtc attcgtcttc ggcgaggacg gtttccaatg gaaccgtttt gccctcgcgg
     2761 atgaccgctt cggtttcctt cacgagccag tccattttcc cggaggcaaa atccgccttc
     2821 atctgcttgt cccattcatc atcggggaag ggatgcaatt cggccatcaa ctcacaatat
     2881 tcctgcggcg agagcttggc gatggcttcc ttgatttcgg caaccgtgga catcagggga
     2941 acattaccga acattccggc gcaaagcaac ccgggaggaa cgccgaagcg cgtgatcgtg
     3001 ggcatccgcg aaccgtggcc ggcctatgta ccggaagacg atcgggcgaa ggcgggaaat
     3061 attcggtccg ttcctgcggg ttggcgttcc gaataggcgc ttcgcgcaga aagaagcggc
     3121 atggctgcct tgcatggatt cgaaccatga cacacagatc cagaatctgc agtgctaccg
     3181 ttacaccaca aggcaatggc cgctcgaaaa gcgcggacgg gaaatctacg ccgctcaatc
     3241 ccgcttgtaa agccgcttgc gcggttattt gtttttatct tcgggaacgg aaccgaagcc
     3301 gatgtcgctg atcccggcct ggcggcagag gtcgagcaca ttcagcatgc gctcgtagga
     3361 aacggtcttg tcggcccgga cgatcaccag ctggtcggga ttcagtttgg cgagcttgcc
     3421 cagcatgtcc gtaaacgcgg tatcatccac cgttttctga ttgaccgtca gggtattgtc
     3481 cgggcggatg ttgacggtga tctgggtggg gctgatgttc cgggcctcgc ctgacttggc
     3541 cgtggggagc tggatgccga tgtcggattc cacctgggtg gtcgcccagg tcatgagcag
     3601 gaacaccaca agcaccatca gaatgtccac cagggaggtg acattaaaaa ggagcggatt
     3661 ctgttggacg agtgttttgc gaaaattcat gccggtggcg gcgtttcccg gatcaaaaca
     3721 aaaatccggc ggaagaaaag cgtttattgc cacggccccg gtcggctatg ccatgatgcc
     3781 cgcttatgcc caagttcctc ttcagtttgc tgctggccgt ttttttctgt tcctgcgccg
     3841 taaccggctc gccgatcggc cagacccagg ttcgcatcaa ggggtatccg gtgtccctcc
     3901 tgcagcagaa aaccggcgag gtgttcaccc gggattcctt catgctcgtg caatcccagc
     3961 cggaccggat ggttttcgag cgcggcggcg gcaacaccga ggaggttttc tacggcgatt
     4021 ggatgaaacg gaacaccgcg gttcgcgtga cggtccattt catccgcaaa ggctcgaacg
     4081 actatctcct gcgcacggac tcccgcatcc ttcgaaatcc cggcagttcg ttcgaggacc
     4141 agagtgatct cttcgacatg cagtccctca aataccggcg ttatctcagc gaggtcaaaa
     4201 aggaactgca gcgcgatttt ccggcggcg
//
LOCUS       DATDAY010000212         2147 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_594978, whole genome shotgun sequence.
ACCESSION   DATDAY010000212 DATDAY010000000
VERSION     DATDAY010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2147)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2147)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2147
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_594978"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(305..1033)
                     /locus_tag="VIS74_04215"
     CDS             complement(305..1033)
                     /locus_tag="VIS74_04215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactose-lipid carrier transferase"
                     /protein_id="HEY5792477.1"
                     /translation="MAKSFPHLDDLDQAKSLEQDEYKQLLKEYQLKLLNAQLILREQK
                     KSVIIVMEGPDAAGKGGAIRRVVERLDPRLTRVYSIVKPTPEEYDHHYLWRFWTKLPS
                     HGQIVIFDRSWYGRVLVERVEGFATEKEWKRAYQEINEFERTLSEDNTAIVKLYLHIT
                     KDEQLARFKCREGDPYKHWKITDEDWRNRKKWDQHNEAAEDMFEKTSTEGAPWKVIPA
                     NFKWYARVETVKAVSKRVAQLIAG"
     gene            1232..>2147
                     /locus_tag="VIS74_04220"
     CDS             1232..>2147
                     /locus_tag="VIS74_04220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792478.1"
                     /translation="MLPLSHYMAVKPSHDMLASTLFARVSSEDIVVFASESLARYLGV
                     PKRDLMGAPLEVVQQRLNGELAAGFSRPLNGRASNQLVTDRAGRVFQLRTATQDGVLD
                     ILLDEVTDVRAVLDRFSASIGMANADLSEEEIQSLFQPERRILSISQTRLCGYSTLAE
                     NAPPFEARFLASAFGEEAGGALLENGSSLGEVSPDAIQGIHGAPRHYRDHALRALKAV
                     FEQFRLTGDFRAHTIRSAKEMPPLAAGISTGEVLLSSLAGSSGQRLAASGSAAALASQ
                     LCRLARPGEILISEPTLFAILAELPPDWE"
BASE COUNT          361 a          674 c          674 g          438 t
ORIGIN      
        1 gatttgccgg atcggcgaat gcgggcggac ttggagcgat tttcaaaaga gatttccgaa
       61 aagggattca atgtaggggc agccggagcg aagaccgcct gtcccgcgga agcgggacag
      121 cccgaggggc gcacgaagtg cggcaattgt gtgccaaccg ccccgcgtaa acggggatgc
      181 ggggtccccg tttccccgca accaaaggcg cccaatgggg tggtggagcc ccatttcccc
      241 accagacaaa cgcctctaca accccttggg tatttcggaa atctcttttg agaaatgcga
      301 taacttaccc cgctatcagc tgcgcgacgc gtttggagac ggccttgacg gtttcgaccc
      361 gcgcatacca cttgaagttg gcgggaatga ctttccacgg cgcgccttcc gtggaggttt
      421 tttcaaacat gtcctcggcc gcttcgttgt gctggtccca tttcttgcgg ttgcgccagt
      481 cctcgtcggt gatcttccaa tgcttgtagg gatcgccttc gcggcacttg aagcgggcga
      541 gctgctcgtc cttggtgatg tggaggtaga gcttcacgat tgccgtattg tcctcgctca
      601 gcgtgcgctc aaattcgttg atttcctgat acgcccgctt ccattccttc tccgttgcga
      661 agccttccac ccgctcgacc agcacacggc cataccacga gcggtcaaag atgacgatct
      721 ggccatggct gggcagcttg gtccagaaac gccagagata atggtggtcg tattcctccg
      781 gcgtcggctt gacgatggaa tagacgcgcg tcagccgggg atcgaggcgt tcgaccaccc
      841 ggcgaatggc gccgcccttg cccgcggcgt cggggccttc catgacgatg atcacggatt
      901 tcttctgttc gcgaaggatg agctgcgcgt tcaggagctt aagctggtat tccttgagca
      961 actgtttgta ttcgtcctgc tcaagcgatt tggcctgatc aaggtcatcg agatgcggaa
     1021 aggatttcgc catgcccggc ttgtaacggc ggcattgcgt tttgggaagc ctggaatcgc
     1081 caaaaacgcc gccgcaaacc gggcgccggc gccgaataaa ccgggccggc cgcgcaccca
     1141 ggtttcgcag gttgcctcgg gtcgccccgc ggtcaattcc caaccttcaa ctcggggctg
     1201 atttggattg cggaatttca gcgttcgcag gatgctgccg ctttcccatt acatggcggt
     1261 aaaaccctcc cacgacatgt tggcgtccac cctctttgcc cgggtgtcgt cggaggacat
     1321 tgtggtcttt gccagcgagt ctctggcccg ttacctcggc gtgcccaagc gcgatcttat
     1381 gggggcgccg ctggaggtgg tgcagcagcg gctgaatggc gaactggccg cggggttttc
     1441 ccgcccgctc aatggccgcg cctccaacca gctcgtgacc gaccgcgcgg gccgcgtgtt
     1501 ccagctccgg accgccacgc aggatggcgt gctcgacatc ctgctcgatg aggtgacgga
     1561 tgtccgggcc gtgctcgatc gtttcagcgc gagcatcggc atggccaacg cggacctgag
     1621 cgaggaggag attcaatccc tcttccagcc cgagcggcgg attttgtcca tctcgcaaac
     1681 gcgcctgtgc gggtattcca cgctggccga aaacgcgccg ccgttcgaag cccggtttct
     1741 ggcgagcgcc tttggcgagg aggcgggtgg ggcgttgctg gaaaatggat cgagcctcgg
     1801 cgaagtctcg ccggacgcga ttcagggcat ccacggcgcg ccgcggcatt accgcgacca
     1861 cgccctgcgc gcgctcaagg cggtattcga gcaattccgg ctgaccggcg acttccgggc
     1921 ccataccatt cgcagcgcca aggagatgcc gccgctggcc gccggcatca gcaccggcga
     1981 ggtgctgctc agctcgctgg ccggctcctc cggccagcgg ctggccgctt cgggttccgc
     2041 cgcggcgctc gccagccagc tctgccgcct ggcgcggccg ggagagattt tgatctccga
     2101 gccgaccctg ttcgccatcc tcgcggaact gcccccggac tgggagt
//
LOCUS       DATDAY010000213         2067 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_1350168, whole genome shotgun sequence.
ACCESSION   DATDAY010000213 DATDAY010000000
VERSION     DATDAY010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2067)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2067)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2067
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1350168"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..802)
                     /locus_tag="VIS74_04225"
     CDS             complement(<1..802)
                     /locus_tag="VIS74_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006983568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HEY5792479.1"
                     /translation="MSSKRVSLYDTTLRDGTQGTGISFSVPDKIRVARKLDDFGMDYI
                     EGGWPGSNPRDAAFFEEAAKLTWKHAKLAAFGSTRRAKVRAEEDSQVRMLLDARTPVV
                     TIVGKTWPLHVTEVLGISLEENLAMIEDTVRYLKAQGREVFYDAEHFFDSYKESPEYS
                     LETLRAAHRGGADVLVLCETNGGNLPDFVGEATRAVLEAIPGAQVGIHTHNDGGVGVA
                     NALAAIRAGASQVQGTVNGYGERVGNCNLTTIIPNLQLKMGIPVVPDLT"
     gene            complement(852..1889)
                     /locus_tag="VIS74_04230"
     CDS             complement(852..1889)
                     /locus_tag="VIS74_04230"
                     /inference="COORDINATES: protein motif:HMM:TIGR00730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEY5792480.1"
                     /translation="MSTPFEAGQVVLREKIKTLVGEVAEGRQAELISELVETALMLGH
                     DRAGIADLKLLRRAFKEMRKAAKLFAGYSDKRKVSVFGSARTKPEAEEYKVAVEFAQR
                     IVQEGFMVVTGGGDGIMGAAQVGAGAANSFGLNIRLPFEQRANETIHGDPKLINFNYF
                     FTRKLSFMKESHAFVLLPGGFGTQDEGFECLTLMQTGKTQVVPVILLDRPNGYYWETW
                     RRFLDNDLLENGLISSTDFNLFRISYDVDDVVKEILKFYSVFHSYRWVREEMVVRLNQ
                     TLTPAALESLNDQFVSLLASGKIVQQAAHPEEKEETHLAHLSRLVLTPRKRDFGRIRL
                     LINEINNSATA"
BASE COUNT          372 a          630 c          639 g          426 t
ORIGIN      
        1 gcgtgaggtc cggcacgacg gggatgccca tcttcagctg gagattcggg ataatggtcg
       61 tgagattgca gtttccgacc cgttcgccgt agccgttgac cgttccctgg acttggctcg
      121 cgccggcgcg aatggcggcc agcgcattgg cgacccccac cccgccatcg ttatgcgtgt
      181 gaatgccgac ctgggcgccg ggaatggctt cgagcacggc gcgggtggcc tcgccgacga
      241 agtcgggaag gtttccgcca ttggtctcgc agagcacgag cacgtccgcg ccaccgcgat
      301 gcgccgcccg cagcgtttcg agcgaatact ccggggattc cttgtagctg tcgaaaaaat
      361 gctccgcgtc gtagaacacc tcgcggccct gcgctttcag ataccggacc gtgtcctcga
      421 tcatcgccag gttttcctcc agcgaaatgc ccagcacttc ggtcacgtgg agcggccagg
      481 tcttgccgac gatggtcacc acgggcgtcc gcgcgtcgag cagcatgcgg acctgcgaat
      541 cctcctcggc gcgaactttc gcccggcgcg tgctgccaaa ggcggccagc ttcgcgtgtt
      601 tccaggtcag cttcgcggct tcctcgaaga aggcggcgtc ccggggattc gaacccggcc
      661 agccgccttc gatgtaatcc atgccgaagt cgtcgagctt ccgcgccacc cgaatcttgt
      721 ccggcacgct gaacgaaatg ccggtgccct gcgtgccatc acgcagggtg gtatcataga
      781 gactgacgcg tttggaagac atgcgggagt gacgacccta gagaaagccg gggcgcgtgg
      841 caatcgcgca ctcaggcggt ggccgagtta ttgatttcgt tgatgagcag ccggatcctt
      901 ccgaagtcgc gcttgcgcgg cgtcagcacc aggcggctga ggtgcgcgag gtgggtttcc
      961 tccttttcct cggggtgcgc ggcctgctgg acgatcttgc ccgacgcgag cagcgagacg
     1021 aactggtcgt tgagcgattc gagcgccgcg ggggtcagcg tttgattcaa ccgcacgacc
     1081 atttcctccc gcacccagcg gtaggaatgg aagacgctgt agaacttcag aatctccttc
     1141 acgacgtcgt ccacgtcgta gctgatgcgg aagagattaa aatcggtgga tgaaatgagg
     1201 ccgttttcga gcaggtcgtt gtcgaggaag cggcgccagg tttcccagta gtagccgttc
     1261 ggccgatcca gcaggatcac ggggacgacc tgcgtcttgc cggtctgcat gagcgtgagg
     1321 cattcaaatc cctcgtcctg cgtgccaaat ccgccgggca gcagcacgaa ggcgtgcgat
     1381 tccttcatga agctgagctt tcgggtgaaa aagtagttga aattgatgag cttcggatcg
     1441 ccgtggatgg tttcattcgc gcgctgctcg aacggcaggc ggatgttcaa cccgaagctg
     1501 ttcgccgcgc ccgcgcccac ctgggcggcg cccatgatgc catccccccc gccggtgacg
     1561 accatgaagc cttcctggac gatccgttgg gcaaattcga cggcgacctt gtattcctcc
     1621 gcttccggct tggtccgggc ggacccgaag acggacacct tgcgtttgtc ggaataaccc
     1681 gcgaacagct tggccgcctt gcgcatttcc ttgaaggccc ggcgcagcag cttcagatcg
     1741 gcgatgcccg cccggtcgtg gccaagcatg agggcggttt ccaccaattc ggaaatcaat
     1801 tccgcctggc ggccttcggc gacttcgcca acgagggttt tgattttttc gcgcaggacc
     1861 acctggccgg cttcaaaggg agtggacata tcttcgggca gcttttgctt attcgtgaaa
     1921 acccgacact ctgccacgga ttccccgggt gacaaacagg aaaaacccgc gcgcgaatga
     1981 ctggactcag gcgggagttg ttttacgttg tcccaattcc aacctgatga aactttttcg
     2041 acgtttctta tttttgctgc ccatcgg
//
LOCUS       DATDAY010000214         2425 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_2124233, whole genome shotgun sequence.
ACCESSION   DATDAY010000214 DATDAY010000000
VERSION     DATDAY010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2425)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2425)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2425
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2124233"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..228
                     /locus_tag="VIS74_04235"
     CDS             <1..228
                     /locus_tag="VIS74_04235"
                     /inference="COORDINATES: protein motif:HMM:NF027219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc-binding metallopeptidase"
                     /protein_id="HEY5792481.1"
                     /translation="LALQPRRAEDPLLPNSADIFRNKETTFDKMIAAWFPLTHVLNNL
                     NRGLGLLDGYPFITSAPAIEKLRFIHETIQG"
     gene            complement(311..628)
                     /locus_tag="VIS74_04240"
     CDS             complement(311..628)
                     /locus_tag="VIS74_04240"
                     /inference="COORDINATES: protein motif:HMM:NF037832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5615 family PIN-like protein"
                     /protein_id="HEY5792482.1"
                     /translation="MKVLFDQGTPAPLRKFLIQHEVTTVHEMGWGLLENGDLLRSAEQ
                     NGYEILVTTDQNLKYQQNLQERKIAIVVLLSTSWPRIRKQTEKVVEAMDLSVPSGYVE
                     VVV"
     gene            complement(634..855)
                     /locus_tag="VIS74_04245"
     CDS             complement(634..855)
                     /locus_tag="VIS74_04245"
                     /inference="COORDINATES: protein motif:HMM:NF016167.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HEY5792483.1"
                     /translation="MDWSTCEGVERSPLKVSGSWLFSGTRVPVVSLFQNLEDGATVED
                     FLNWFPGVSRQQVEAVLEFAEKSLLVEAV"
     gene            complement(960..1433)
                     /locus_tag="VIS74_04250"
     CDS             complement(960..1433)
                     /locus_tag="VIS74_04250"
                     /inference="COORDINATES: protein motif:HMM:NF025703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDYXXLXY domain-containing protein"
                     /protein_id="HEY5792484.1"
                     /translation="MKRIPLYLVLALQVIGLISLYAYYSSEKSLPGYLLRTRPVDPRD
                     LLRGDYVILSYDISMLPEGSKSEDFPGGQAYVTLRPEGEFWKIGAVSAKSPETNEPVL
                     KAQVKGRALVYDIEKYFVPEGMGNPPLPITVEIVIRTGGRAQIRQLYADGQRWPW"
     gene            complement(1430..2365)
                     /locus_tag="VIS74_04255"
     CDS             complement(1430..2365)
                     /locus_tag="VIS74_04255"
                     /inference="COORDINATES: protein motif:HMM:NF021446.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2157 domain-containing protein"
                     /protein_id="HEY5792485.1"
                     /translation="MKLHQAKLKSEVDAWITAGVISRGQGDQILARYPAEARNYWLAA
                     LVVIGSVLCLGGVILIIASNWREIPALAKLIGLLALLIGSTVTGIESARGGAPRVASE
                     CGFLGAAVFPLLGLALISQIFHLDGQMSTLLLAWFAAIAPLPFLSRSLSSWVVWIIAG
                     GWLLGALITENVSNPGFAWFCGAYIGFGIGLALVSQLWRPHPQRTVGEFIGVLTAGLA
                     AYLMAFDVTHWFLLWCAVFAAALGLIYWGYRTARVHQVNFAFVLVALVLLSTFLRLVG
                     TMAKTGTIFLAGGVFMILLAWGLNHLRRKVIRRIQ"
BASE COUNT          546 a          736 c          697 g          446 t
ORIGIN      
        1 ctcgcgctcc agccacgccg ggcggaggat ccgctgctgc cgaattccgc ggacatcttt
       61 cggaacaagg agacgacttt cgataaaatg atcgccgcct ggttcccgct cacccacgtg
      121 ctgaacaatc tcaaccgcgg cctggggctg ctcgacggtt atcccttcat cacgtccgct
      181 ccggcgattg aaaaactgcg gttcattcac gagacgattc agggctgatc tcacgcggag
      241 acgcaaagcc gctcaatgaa tttgcctttt ctccgcggct ttgcgtctcc gcgtgagttt
      301 attttgagtt ctatacgact acttcgacgt agccacttgg aaccgataaa tccatggctt
      361 cgacaacctt ttcggtctgt ttccggattc gcggccaact ggtggagagc aaaaccacga
      421 tggcaatttt tctttcctgc agattctgct gatatttcaa attttgatcg gtggtaacga
      481 ggatttcgta accgttttgt tcggcagatc gaagtaaatc gccgttttcc aataagcccc
      541 atcccatctc gtggacggtg gtgacttcgt gttggatcaa aaattttcga agcggcgccg
      601 gagtgccctg atcaaacaaa accttcatcc aagctaaacg gcttctacca gcaggctctt
      661 ttctgcaaac tccagaacgg cttcaacctg ctgacgagag acgccgggaa accagttcaa
      721 aaaatcttca accgtagctc catcctccaa attctgaaaa agagagacaa ccggaacgcg
      781 ggttcccgaa aataaccagc ttccgcttac ctttaaaggt gagcgttcca ccccttcgca
      841 tgtggaccag tccaacacaa aatccacctt aatttaaaag cccggcgcga gcaagtgcgg
      901 ggttggaggc cggatcaagc ccctccgcgg ctttgcgcct tgcgggagtg aacggtcttt
      961 taccagggcc accgctggcc gtcggcgtaa agttgccgga tctgcgcccg gccgccggtg
     1021 cggatgacga tttccaccgt gatcggcagc ggcggattgc ccatgccttc gggcacgaaa
     1081 tacttttcga tgtcgtaaac cagcgcgcgg cccttgacct gcgccttcaa caccggctca
     1141 ttcgtctccg gagacttcgc ggaaaccgcg ccgatcttcc agaattcgcc ctccggccgg
     1201 agcgtgacat aggcctggcc gcccgggaaa tcctccgatt tcgaaccctc cggcagcatg
     1261 ctgatgtcgt aactcagaat cacgtaatcc ccgcgcagca aatcccgcgg gtccaccggc
     1321 cgggtgcgca gcaggtaacc gggcagggat ttttccgacg aataataggc gtagagcgaa
     1381 atcaggccga tgacctgaag cgccagcacg aggtagagcg ggattcgttt cattggatgc
     1441 ggcggatgac cttgcggcga aggtgattga ggccccaggc gaggagaatc atgaagaccc
     1501 cgccggcgag gaaaatggtt ccggttttgg ccatcgtccc caccaggcgg aggaaggtgg
     1561 agagcagcac cagcgcgacg aggacgaagg caaagttcac ctgatgaacg cgggcggtcc
     1621 ggtaacccca atagatcagg ccgagggccg cggcgaagac cgcgcaccac agcagaaacc
     1681 agtgcgtcac gtcgaacgcc atcagataag cggccagacc ggccgtcagc acgccgataa
     1741 attcaccgac ggttcgctgc ggatgtggcc gccagagctg tgacacgagc gcgagcccga
     1801 tgccgaagcc gatgtaggcg ccgcagaacc aggcgaagcc cgggttgctc acattttccg
     1861 tgatcaaggc tcccagaagc cagccgccgg cgatgatcca gaccacccac gaactcaggc
     1921 tgcgcgagag aaacggcagc ggagcaatgg cggcaaacca cgccagcaga agcgtgctca
     1981 tttggccatc cagatggaaa atctggctga tcaatgccag cccgagcagg gggaagaccg
     2041 cggcgcccag gaagccgcat tcggacgcca cgcgcggagc cccgccgcgg gccgattcga
     2101 tgccggtcac ggtcgaacca atcagcaggg ccagcaggcc gatgagtttg gccagcgcgg
     2161 gaatctcccg ccaattgctc gcgatgatca aaatgacgcc gccgaggcag agcacgctgc
     2221 cgatcaccac cagcgcggcg agccagtaat tgcgcgcctc ggcgggataa cgcgcgagga
     2281 tttgatcgcc ctgcccgcgg gagatcacgc ctgccgtgat ccaggcgtcc acttcggatt
     2341 taagtttggc ttgatgaagt ttcatgaaag aagcgcatgg cgaacccatt ccggcggcgc
     2401 tgccggcggc gccggcggaa ccgaa
//
LOCUS       DATDAY010000215         2542 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_8694, whole genome shotgun sequence.
ACCESSION   DATDAY010000215 DATDAY010000000
VERSION     DATDAY010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2542
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8694"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..483
                     /locus_tag="VIS74_04260"
     CDS             <1..483
                     /locus_tag="VIS74_04260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792486.1"
                     /translation="TTLQPPTAPGIPKPPTFPGAPKPPGPPAAPVAPPPLKPLGAAPS
                     APAAPSAPATPAPTAPAPVPKPASMGPAPAAVKPQAKKETAKVAATPGFAPKPQATVR
                     LQPRPVPSQLSSASFNVQTIEPAVAAETTEEEIPMNFAIAAVVTAVIALAIQVLIAAA
                     "
     gene            558..884
                     /gene="trxA"
                     /locus_tag="VIS74_04265"
     CDS             558..884
                     /gene="trxA"
                     /locus_tag="VIS74_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEY5792487.1"
                     /translation="MASTNVIEFTDANFSTEVLAASTPVLVDFWAPWCGPCRMIAPLI
                     DEIADEKAGVIKVGKVNVDDNPEIAAKFRIQAIPTILIFKNGEKKDQITGLTSKGDLV
                     AKLDAA"
     gene            1248..1586
                     /locus_tag="VIS74_04270"
     CDS             1248..1586
                     /locus_tag="VIS74_04270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792488.1"
                     /translation="MGEAVARTASPNQDASRPPRGGRWKGYFFFGAAFFAAGFFAAGF
                     ATAFLATGFLAGAFLAGAAFLAGAFFTAGFAAALAGAFLATGFLAAGFLTAIRYPPFL
                     SVGAESVKNL"
     gene            complement(1810..2385)
                     /locus_tag="VIS74_04275"
     CDS             complement(1810..2385)
                     /locus_tag="VIS74_04275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavoprotein"
                     /protein_id="HEY5792489.1"
                     /translation="MSDPKTSMNAGKTIVLGVGGSIAAYKAADLTSRLAKRGFDVHVV
                     MTRAATEFVTPLTFQTLSRNPVTTSIFDEKESWHPGHIDLADRAALLLIAPATANLVA
                     KLANGIADDALTSIALATPAPLLIAPAMNGKMWLHAATRENVARLRARDVEFIGPEEG
                     LLACGYEGIGRLWNVEDIAARAEKILHPKSA"
BASE COUNT          442 a          780 c          796 g          524 t
ORIGIN      
        1 acgactctcc agccgcccac ggctcccggg attcccaagc cgccgacttt tcccggcgct
       61 ccgaagcctc ccggcccgcc tgcggctccc gtcgcgcctc cgccgctcaa gccgttgggc
      121 gccgcaccga gcgcccctgc cgcaccgagc gcgcccgcaa cgccggcgcc cactgcgcca
      181 gcgcccgtgc cgaaaccagc ctccatgggg cccgcgcccg ccgcggtgaa accgcaggcg
      241 aaaaaggaaa ccgccaaggt tgccgccacg ccgggtttcg caccgaaacc ccaggccacc
      301 gtgcgtctgc agccccggcc cgtgccgtcc cagctctcca gcgcctcatt caacgtccag
      361 accatcgaac ccgccgtcgc cgcggaaacc accgaagaag aaattcccat gaactttgcc
      421 atcgccgccg ttgttaccgc cgtcatcgcc ctcgccatcc aggtcctgat cgcggccgcc
      481 tgatttctga ttctttaatt ccatcggatc gcccgggaag ctccgctgcc cgcgacgaac
      541 caagccaatt tttatttatg gccagcacaa acgtcatcga attcaccgac gcaaactttt
      601 ccaccgaggt tcttgccgcc agcacgcccg tgctcgtgga cttctgggcc ccgtggtgcg
      661 gtccctgccg catgatcgcc ccgctcatcg acgagatcgc cgatgaaaaa gcgggcgtca
      721 tcaaggtcgg caaggtcaac gtggatgaca atcccgagat cgcggccaag ttccgcatcc
      781 aggcgattcc caccatcttg atttttaaga acggcgaaaa gaaggaccag atcaccggtc
      841 ttaccagcaa gggcgacctc gtcgccaagc tggacgcagc ctgatcgcgg cgcccaggtt
      901 tcgtcaaaag tccccgctcg cgcgggggct ttttggtttc tgaaccggac ggggccgttc
      961 catcgagccg atttgcgcgc actgcggctt ataccaattc cagtaaagaa tcggattatc
     1021 cgcagattac gccgattggc gcagatttta acgacaagaa ggaattcctt tctgcgtcaa
     1081 tctgtgagct ggagcgcagc ggagacagac gaagtcaaat ctgcggatga ccccggccgc
     1141 cgtcttgagt ttctacgccg tctcggagtt ccagattcag tccagatcat tcctcgattc
     1201 ggtattaccg gagagggatc gcgcagcgaa acctgacccc aaagcaattg ggagaagccg
     1261 tcgcgaggac tgcttctccc aatcaggatg cttcacgccc accgcgaggc gggcgatgga
     1321 agggttattt tttcttcgga gccgcttttt tcgcggcggg gttcttcgcc gctggtttcg
     1381 cgacggcctt cttggcgacg ggcttcttgg cgggagcctt tttagccggg gccgcttttt
     1441 tggcgggagc cttcttcact gccggtttcg cggcggcttt ggccggggct tttttggcga
     1501 caggtttctt ggctgcaggt tttttaactg ccattcgata tccccccttt ctttccgtag
     1561 gtgcggaaag tgtgaaaaac ttgtgaataa ctcactactg ctgtggataa atttttgatc
     1621 caaatgcgtc aacgaatttc tctattttcg cttgattttt tcgggtgaaa tttttggtgc
     1681 gcggaaagcc cggcgcgaga cagtcgcccg gcgacgcagg aaacatgcgg ctggggaggg
     1741 atccttgtgc gcggacggga cgaatcggct tcgtggagag acgaagagcg gattcgtgcg
     1801 cgcgcggatt catgcagact tcgggtggag aattttttcc gcgcgcgcgg cgatgtcctc
     1861 gacgttccac agccggccga tgccctcgta accgcaggcg agcaggcctt cctcggggcc
     1921 gatgaattcg acgtcgcggg cgcgcaggcg cgcgacgttt tcccgcgtgg cggcgtggag
     1981 ccacatcttg ccgttcatgg ccggggcgat gagcagcggc gcgggcgtgg cgagtgcgat
     2041 cgaggtgagc gcgtcgtcgg cgatgccgtt tgcgagcttg gcgacgaggt tcgcggtggc
     2101 gggcgcgatg aggaggaggg cggcgcggtc ggcgagatcg atgtggccgg gatgccacga
     2161 ttccttttcg tcgaagatgg atgtggtgac ggggttgcgg gagagcgtct ggaacgtgag
     2221 cggggtgacg aactcggtcg cggcgcgcgt catgacaacg tgcacgtcga agccgcgttt
     2281 cgcgaggcgg ctggtgaggt ccgcggcctt gtaggcggcg atggagccgc cgacgccgag
     2341 gacgatggtt tttccggcgt tcatgctggt cttcgggtcg ctcatgctga tcttcgggtc
     2401 gcttatgcgg cgggatggta aatgagccgt cggctgaggt aacgctcggc gcgcatgacg
     2461 gcgcggaatt tttcgatgtc ctcctccatg gagccgctga tgagcgtgta gcgatattcg
     2521 cgccaatgcc gcatctccgc ag
//
LOCUS       DATDAY010000216         2470 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_2521102, whole genome shotgun sequence.
ACCESSION   DATDAY010000216 DATDAY010000000
VERSION     DATDAY010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2470)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2470)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2470
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2521102"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..285
                     /locus_tag="VIS74_04280"
     CDS             <1..285
                     /locus_tag="VIS74_04280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792490.1"
                     /translation="NFNAFPGRAEPPPVTRFIELNFDAHVSENTPETTAGTYPVGKIW
                     IGTTRAGKAFARVNNAPELVELEASGADALIKSASQSAPAEANPVPSPTP"
     gene            282..>2470
                     /locus_tag="VIS74_04285"
     CDS             282..>2470
                     /locus_tag="VIS74_04285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase-related protein"
                     /protein_id="HEY5792491.1"
                     /translation="MTSAEIRNSFLEFFREQQHTIVPSSPLMPDSPNLLFTNAGMNQF
                     VPIFLGQQKCPYIPGRAADTQKCIRAGGKHNDLDDVGLDTYHHTFFEMLGNWSFGDYF
                     KKEAIAWAWELVVNRWKFPAERLYATVYNPAPGDPSEFDQVAHDLWAKRFAAAGLDPK
                     IHIVNGNKKDNFWMMGDTGPCGPCSELHVDLTPAGDTKGSLVNQGDARCMEIWNLVFI
                     QFNANPDGTFSPLPAQHVDTGMGFERVTSIIQGTKNFTDFATAKISNYETDIFRPIFD
                     ELEKLSGRKYTSTLPSGSVENLGAQVETDIAFRVIADHIRTLSFAIADGILPGNNDRN
                     YVLRRILRRAVLFGRKLGFHEPFFYKLSPILAEQMGDVFPEIRARQAPIQSVLQQEEE
                     AFNKTIDRGIKMFEQEVFARTLAAAAQHEGLAMVQSSKSFWVPDGKNPPEEVDVLEAR
                     FLRGEEVVGDFLFEELLQGAWRQVLKSKPRISGDDAFKLYDTYGFPLDLTELMAHERG
                     LEVDKEGFEALMEQQRDRARAAQKKEVITAESETHDLETRFVGFDTLQTEAHLVDVVS
                     RGGDTFAVVDVSPFYAEMGGQVGDTGTLEFGGSLVPVSNTLKRGHTFYLKIPSALTDL
                     SAPSAVKLSVDAPRRRAIERHHTVTHLLHWALHEVVSKDASQKGSHVAPDKLTFDFNS
                     AALTPPQVRAIEKLVNERILEDAAVSWIEVPYADVKPRRDIQQFFGD"
BASE COUNT          500 a          870 c          682 g          418 t
ORIGIN      
        1 aactttaatg cctttccggg ccgcgccgaa ccgccgcccg tgacccggtt catcgagttg
       61 aatttcgacg cccacgtttc ggaaaacact ccggaaacca ccgccggcac ctaccccgtc
      121 ggcaaaatct ggatcgggac cacccgggcg ggaaaagcct tcgcccgggt gaacaacgcc
      181 cccgaactcg tcgaactcga agcctccggc gcggatgccc tcataaaatc cgcttcccaa
      241 tccgcccccg ctgaggcaaa ccctgtccct tcgcccacgc catgacgtcc gccgaaatcc
      301 gcaactcttt cctcgaattc ttccgcgagc agcagcacac gatcgtgccc agcagcccgc
      361 tgatgccgga ctcccccaac ctgcttttca ccaatgccgg catgaaccag ttcgtgccca
      421 ttttcctcgg ccagcagaaa tgcccctaca tccccggacg cgccgccgat acgcagaaat
      481 gcatccgcgc cggcggcaag cacaacgacc tcgatgatgt cgggttggat acctaccatc
      541 acaccttctt cgagatgctc ggcaactggt ccttcgggga ttacttcaag aaggaagcca
      601 tcgcctgggc ctgggagctc gtggtgaacc gctggaagtt cccggcggag cggctttacg
      661 cgacggttta caatcccgcc cccggcgacc cgagcgaatt cgaccaggtg gcccatgacc
      721 tctgggccaa acgcttcgcc gccgccgggc tcgatccgaa gatccacatc gtcaatggca
      781 acaagaaaga caacttctgg atgatgggcg acaccggccc gtgcggcccg tgttccgaat
      841 tgcacgtgga cctcacgccc gccggcgaca cgaagggctc gctcgtgaac cagggcgacg
      901 cccgctgcat ggaaatctgg aatctcgtgt tcatccaatt caacgcgaat cccgacggca
      961 ccttctcccc gttgcccgcg cagcacgtgg acaccggcat gggcttcgag cgcgtgacct
     1021 ccattatcca gggcacgaaa aatttcaccg acttcgccac cgcgaaaatc tcgaactacg
     1081 agaccgacat cttccgcccg attttcgacg agctggaaaa actcagcggc cgcaaataca
     1141 caagcacgct gccgtccggt tcggtggaaa atctcggcgc ccaagtcgaa accgacatcg
     1201 ccttccgcgt catcgccgat cacatccgca cgctgagctt cgccattgcc gacggcatcc
     1261 tgcccggcaa caacgaccgc aactacgtgc tgcgccgcat tctccgccgc gcggtgctct
     1321 tcgggcgcaa gctcggattc cacgaaccct tcttctacaa gctctccccg atcctcgcgg
     1381 agcaaatggg cgacgtcttt ccggaaatcc gcgcgcggca ggcgcccatc cagtccgtgc
     1441 tgcagcagga agaggaggcg ttcaacaaga ccatcgatcg cggcatcaag atgttcgagc
     1501 aggaggtctt tgcccgcacg ctcgccgccg ccgcccagca cgaggggctg gcgatggtgc
     1561 aatcctccaa atccttctgg gtgcccgatg gcaaaaaccc gcccgaggaa gtggacgtgc
     1621 tcgaagcgcg tttcctgcgc ggcgaggaag tcgttggcga tttcctcttt gaggagctgc
     1681 tccagggcgc ctggcgccag gtgctgaaat ccaagccgcg catctccggc gacgacgcgt
     1741 tcaagctcta cgacacctac ggtttcccgc tcgacctgac cgaactcatg gcccacgaac
     1801 gcgggctgga ggtggacaag gaaggcttcg aggcgttgat ggaacagcag cgcgaccggg
     1861 ctcgcgctgc tcagaagaag gaagtcatca ccgccgagtc cgaaacgcac gatctcgaaa
     1921 cgcgcttcgt cggcttcgac acgctgcaaa ccgaagcgca cctcgtggac gtcgtctcgc
     1981 gcggcgggga caccttcgcc gtggtggacg tttcgccctt ctacgccgaa atgggcggcc
     2041 aggtcggcga caccggcacc ttggagttcg gcggctccct cgttcccgtt agcaacacgc
     2101 tgaagcgggg gcacacgttt tacctcaaaa tcccctccgc cctcaccgat ttgtccgctc
     2161 cctccgcggt caaactttcg gtggacgccc cgcgccgccg ggccatcgag cggcaccaca
     2221 ccgtcacgca cctgctccat tgggcgctgc acgaggtcgt cagcaaggat gcctcgcaaa
     2281 agggctcgca cgtcgcgccg gacaagctca cgttcgactt caacagcgcc gcgctgacgc
     2341 cgccgcaggt gcgcgccatt gaaaaattgg tcaatgaacg catcctcgaa gacgccgcgg
     2401 tgagctggat cgaggtgccc tacgcggacg tcaaaccgcg ccgggacatc cagcaattct
     2461 tcggcgacaa
//
LOCUS       DATDAY010000217         2166 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_1085376, whole genome shotgun sequence.
ACCESSION   DATDAY010000217 DATDAY010000000
VERSION     DATDAY010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2166)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2166)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2166
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1085376"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..472)
                     /locus_tag="VIS74_04290"
     CDS             complement(<1..472)
                     /locus_tag="VIS74_04290"
                     /inference="COORDINATES: protein motif:HMM:NF013073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HEY5792492.1"
                     /translation="MNLRLALTLLMLTFGDLRAEIPELDLPADLAPERRALVELSLRE
                     LAAHPNVPYRYAAASPEEGMDCSGAIYYLLRLAGCEPPRSSAAQYEWVKAAGNLTAVP
                     PDGRSLDHPAFAKLRPGDLVFWAPDGPDSAAHPQVTHVQIYLGREKRDGFPVMIG"
     gene            608..1954
                     /locus_tag="VIS74_04295"
     CDS             608..1954
                     /locus_tag="VIS74_04295"
                     /inference="COORDINATES: protein motif:HMM:NF018032.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HEY5792493.1"
                     /translation="MEDAICLLGLDVGSTTTSLLLARVPRVRNCVTGRSEFGAPQFLA
                     QPEAVFTPFNGERIDGAALERQLDEWLADPALPPIEAGGILLTGLAAQSANAAALRER
                     LRRRLGDALVAAAPDPRLESWLAFHGGCGRLSAARPEEIFLNLDIGGGTTNLALGRAG
                     EVRATASLWIGARHVGFDPGTRRVRAASAVGRRLLEGVSAKAGDVLREAQVKDIAEFC
                     ARRLRDFAAGRSIEDWLVDAPWNAPPDPPGQILLSGGVGELFYQCRAGADFPIPSPFG
                     DLGADLARALADSLPASADIPPHRGRATVMGITLHSTEISGATIYVSPRVDLPLEEVP
                     ILGELAFAGDRPALPGEKSGVIGLRLAGAPPSAEALKSLAARLGRRLDETGSPVLVLL
                     LESDLGKTLGSYLTDWGNAPRRVVVLDELSVPRSARFVRVGARRASGVPVIFYGLV"
BASE COUNT          328 a          743 c          722 g          373 t
ORIGIN      
        1 aaccgatcat gacgggaaat ccatcccgct tctcgcgtcc gaggtaaatc tgcacgtggg
       61 tcacctgcgg atgcgccgcg gaatccggcc cgtccggcgc ccagaacacc aaatcgcccg
      121 gccgcagttt cgcgaatgcc ggatgatcca gcgaacgccc gtccggcgga accgccgtca
      181 ggttgcccgc ggccttgacc cattcgtatt gcgcggcgga actgcgcggc ggttcgcagc
      241 ccgccagccg cagcagataa taaatcgcgc cggagcaatc catgccctcc tccggcgagg
      301 cggcggcgta gcgatacggc acatttggat gcgcggcgag ctcccgcagc gaaagctcga
      361 ccaacgcccg gcgctcgggc gccaaatccg ccggcagatc cagctccgga atttccgctc
      421 gcaggtcgcc gaacgtcagc atgagcaggg tcaaagcgag gcggagattc atgatatttc
      481 taaagggaaa tccaatcatt tcacgcaaag ccgcaaaaaa gcggtgtttc ttgaaaacct
      541 ttgcgtgagg ctcaatgcct ttaaaattcg ggtttcgccg ccgctccggt cgtcctatgt
      601 tgcgcgaatg gaggacgcca tctgcctgct cggccttgac gtgggctcga ccacgacaag
      661 cctgctgctg gcgcgcgtgc cgcgcgtgcg gaattgcgtc accggccgga gcgaatttgg
      721 cgcgccgcaa tttctcgccc agccggaagc cgtcttcacg ccattcaatg gagagcgcat
      781 cgatggcgcc gcgctcgaac gccagctgga cgaatggctc gccgatcccg cgctgccgcc
      841 gatcgaggcg ggcggcatcc tcctcaccgg cctggcggcg caatccgcca acgcggcggc
      901 attgcgcgag cgcctgcgcc ggcggctggg cgacgcgctc gtggccgccg ctcccgatcc
      961 gcggctggaa tcctggctcg cctttcacgg cggctgcggg cggctttccg ccgcgcgccc
     1021 tgaggagatt tttctgaatc tcgacatcgg cggcggcacc acgaatctcg ccctcggccg
     1081 ggcgggcgag gtgcgggcca cggcgtccct gtggatcggc gcgcggcacg tgggatttga
     1141 ccccggcacg cgccgcgtgc gggcggcgtc cgccgtgggc cgtcgcttgt tggaaggcgt
     1201 ttcggcgaag gccggcgacg ttctgcggga agcgcaggtc aaggacatcg ccgaattctg
     1261 cgcgcggcgc ttgcgcgatt tcgccgcggg ccggtccatc gaggactggc tggtggacgc
     1321 gccctggaat gcgccgccgg acccgcccgg gcaaatcctg ctctccggcg gagtggggga
     1381 attgttttat caatgccgcg ccggcgcgga ttttcccatt ccctccccgt ttggcgacct
     1441 cggagcggac ctcgcgcggg cgctggcgga ctccctcccg gcttccgcgg acattccgcc
     1501 gcatcgcggc cgcgccaccg tcatgggcat cacgctgcac agcaccgaga tcagcggcgc
     1561 gaccatttac gtgagtccca gggtggatct gccgctggag gaagtcccga tcctcggcga
     1621 actggccttc gccggggacc ggcccgcgct gccgggggaa aaatccgggg tgatcggcct
     1681 ccgcctggcc ggcgcgccgc cgtccgccga ggcgctcaag tccctggccg cgcggctggg
     1741 tcggcggctg gacgaaaccg gctcgccggt cctggtgctc ctgctggaat ccgacctcgg
     1801 caagacgctg ggcagttacc tcaccgattg gggcaacgcc cctcggcggg tcgtggtgct
     1861 cgatgaattg tccgtcccgc gttcggcgcg gtttgtgcgc gtgggcgcgc gccgcgcctc
     1921 gggcgtcccg gtgatttttt acgggctggt ttgatcccgc gcttttcatt cggggaaaat
     1981 gcattaaatt ccccgcattc ggggcagcgg caaggcaagc caatgaaggc gattcgcgac
     2041 attccagttc cgccacccca gcccgacgtg cctttttcca cgcggctgct ggatcggacc
     2101 ttcgtcttcc ccagcctcaa gcggctgctc ggcgcggccg attacggcaa ggcgggggat
     2161 cgcaac
//
LOCUS       DATDAY010000218         2606 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_526658, whole genome shotgun sequence.
ACCESSION   DATDAY010000218 DATDAY010000000
VERSION     DATDAY010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2606
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_526658"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            121..903
                     /gene="larE"
                     /locus_tag="VIS74_04300"
     CDS             121..903
                     /gene="larE"
                     /locus_tag="VIS74_04300"
                     /inference="COORDINATES: protein motif:HMM:TIGR00268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HEY5792494.1"
                     /translation="MREKLTRLSEELRANAPLAIAYSGGVDSACLLALAHRELGAQAL
                     GVIADSPSLPRRALAEALAAAEGFGAKVEVVRTREFENPDYAANPLNRCYFCKAELFQ
                     RMDALARERGFRAIAYGENADDPAGSRPGSLAAREFRVLAPLKTAGLGKAEVRTLARE
                     LGLRAADAPSQPCLSSRVATGVPVTPPVLSQIEQAEEILRGRGFRILRVRFLEGGAAR
                     IQVGPEELARLFRTRAEVLAALEPVGFSRLELDPQGYRGVIA"
     gene            907..1515
                     /locus_tag="VIS74_04305"
     CDS             907..1515
                     /locus_tag="VIS74_04305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792495.1"
                     /translation="MTTAPRAFVRRLNSHNRHALLGALLSFVASAGVWTGLFWGGQVL
                     IVLALSSIQGPAARLPVAYFWIFCGGVAVLLTLAAIDRTLNPFPRPDDRPIIGWHILK
                     QLALLPALLTFAIYDNLNAYRRLSPEVAGQAWALLTGIYRARKLPYHELGRLGLEASQ
                     AEGLLLLLQFAGYVDLHHGREEWFYLVRSSEEESLKLLAEGN"
     gene            complement(1512..2510)
                     /locus_tag="VIS74_04310"
     CDS             complement(1512..2510)
                     /locus_tag="VIS74_04310"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF002872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003872 - 6-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HEY5792496.1"
                     /translation="MSKKIAVLTSGGDSPGMNPAIRAVARTAQSHGVEAIGISDGYEG
                     IFERAFVDLNERALAEVLDRGGTFLQSSRCKRFYDPSGIDEAVEILREEKIGGLIVIG
                     GDGSLTGAREIARRGFPTIGIPGSIDNDIWGTDMSVGVDTAANTIMAMVDRIKDTARS
                     HRRCFLVEVMGRNSGYLALITAISCGAEVAVIPEFYYNIERIHRLLQHRYEKTKDNSI
                     ILLAEGVTPASEFYERLKEVNGGELKQETRITVLGHVQRGGIPTHFDRVLANRMGELA
                     VVGLTQGETGVMTALKKSRLTLLDLDKVIGIKKHPAPELVRLARNLGIEFGDVVET"
BASE COUNT          424 a          791 c          868 g          523 t
ORIGIN      
        1 atcgtttacg aaatgcgctt cgatgaagtc agcgcgaaat acgcggagtt cggcgaattt
       61 ttcatcgcgc tgcagctccc gctggacgcg ctgctgcggc gcttgagctt gtaacctggc
      121 atgcgggaaa aactcacgcg attatccgag gaactgcggg cgaatgcgcc gctggccatc
      181 gcgtattccg gcggcgtgga cagcgcgtgt ttgctggccc tggcgcaccg ggagctgggc
      241 gctcaggcgc tgggggtcat tgccgacagc ccgagcctgc cgcggcgcgc gctggcggaa
      301 gccctcgccg cggcggaggg gtttggagcg aaggtggagg tcgtccggac ccgggagttc
      361 gagaatcccg actacgccgc gaatccattg aaccggtgtt atttttgcaa ggccgagctg
      421 ttccagcgaa tggacgcgct ggcgcgggag cgcggttttc gcgcgatcgc ctacggcgaa
      481 aacgccgacg acccggcggg atcgcgccct ggctcacttg cggcccggga attccgggtg
      541 ctcgccccgc tgaaaaccgc cggattgggc aaggcggagg tgcggaccct ggcccgggaa
      601 ctcggcctgc gcgcggcgga cgcgccgtcg cagccgtgcc taagctcccg cgtcgccacg
      661 ggcgtgccgg tgaccccgcc ggttctttca cagatcgaac aagccgagga aatcctgcgc
      721 gggcgcggat tccgaattct gcgcgtgcgt tttctcgaag gcggcgcggc gcgcatccag
      781 gtcgggccgg aagaactggc gcggttgttc cgcacgcggg ccgaggtgct cgccgcgctg
      841 gagccggtgg gattttcccg gctggagctg gatccgcagg gctaccgcgg ggtgatcgcg
      901 taatccatga ccacggctcc ccgggcattc gtgcggcggc tcaattcgca caaccggcac
      961 gccctgctgg gcgcgctgct cagcttcgtg gcgtcggcgg gggtttggac ggggctgttc
     1021 tggggcggcc aggtgctgat tgtgctggcg ctgtcgagca ttcagggccc ggcggcgcgg
     1081 ctgccggtgg cgtatttctg gatcttctgc ggcggggtgg cggtgctgct gacgcttgcg
     1141 gccattgacc ggaccctgaa cccgttcccg cggccggatg accggccgat cattggatgg
     1201 cacattctca agcaactggc gctgcttccg gcgctgctga cctttgcgat ctatgacaat
     1261 ctcaacgcat accgccggct gtctccggaa gtcgccggtc aggcgtgggc cttgctgacc
     1321 ggcatctacc gggcgcgaaa gctgccgtat catgaacttg gccggctcgg cctcgaggcg
     1381 tcgcaggcgg aggggctgct gctgttgctg caattcgccg gttacgtgga cctgcaccac
     1441 ggccgggagg aatggtttta cctcgtccgc agttccgagg aggagtcgct caagcttttg
     1501 gccgaaggaa actaagtttc caccacgtcg ccgaattcaa tgcccagatt tcgggcgagg
     1561 cggaccaatt cgggagccgg gtgttttttg atgccgatga ccttgtcgag atcgagcagg
     1621 gtgagccggc ttttcttcaa ggcggtcatt acgcccgttt ccccctgggt caggccgacg
     1681 acggcgagtt cgcccatgcg attggcgaga acccggtcga aatgcgtggg aatcccgccg
     1741 cgctggacat ggccgagcac ggtgatgcgg gtttcctgct tgagctcgcc gccgttgact
     1801 tccttgaggc gttcgtaaaa ttcgctcgcg ggggtcacgc cttccgccag gagaatgatg
     1861 ctgttgtcct tggttttttc gtaacggtgc tgcagcagcc ggtggattcg ttcgatgttg
     1921 taataaaatt cgggaatcac cgcgacctcg gcgccgcagc tgatggcggt gatgagcgcc
     1981 aggtagccgg aattccggcc catgacttcg accagaaaac accggcggtg ggagcgggcg
     2041 gtatccttga tgcggtccac catggccatg atggtattcg ccgcggtgtc cacgccgacg
     2101 ctcatatccg tgccccagat gtcgttgtcg atgctgcccg gaatgccgat ggtgggaaat
     2161 ccccggcggg caatctcgcg cgcgccggtc agcgagccgt cgccgccgat gacgattaat
     2221 cccccgattt tttcctctcg caaaatctcc acagcctcgt caatgccgga gggatcataa
     2281 aaccgcttgc agcggctgga ttggaggaag gtgccgccgc gatccagcac ctcggccagg
     2341 gcgcgttcat tgagatccac gaacgcgcgt tcaaagattc cttcataacc atcggaaatg
     2401 ccaatggctt ccactccgtg actttgagcg gtccgcgcca cggcccggat ggccggattc
     2461 attcccggag agtctccgcc ggaggtcaaa accgcgattt ttttcgacat ggaaaccttg
     2521 gtgcctcaat cccccggaat cggacagaat ttttttatga aattttttga acgccggccc
     2581 cgcccccgcg tctaattcta caggtt
//
LOCUS       DATDAY010000219         2392 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_201438, whole genome shotgun sequence.
ACCESSION   DATDAY010000219 DATDAY010000000
VERSION     DATDAY010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2392)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2392)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2392
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_201438"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            103..333
                     /locus_tag="VIS74_04315"
     CDS             103..333
                     /locus_tag="VIS74_04315"
                     /inference="COORDINATES: protein motif:HMM:NF025482.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4287 domain-containing protein"
                     /protein_id="HEY5792497.1"
                     /translation="MSFQAYLTNIQAKTGKSPADFRALAEKKGFTQNGKLAAGVKAGA
                     IVQWLKDDFQLGHGHAMAIYALLKGVKREESP"
     gene            complement(606..932)
                     /locus_tag="VIS74_04320"
     CDS             complement(606..932)
                     /locus_tag="VIS74_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HEY5792498.1"
                     /translation="MSNPACPMCEMTDVLKHPDRCECATCGHEWKCESQPEAARVVKD
                     AFGTVLADGDSVTLIKDLKLKGSSQVIKGGTKAKGIRLVDGDHEISCKIDGGAIGLKA
                     CFVKKS"
     gene            1361..1579
                     /locus_tag="VIS74_04325"
     CDS             1361..1579
                     /locus_tag="VIS74_04325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792499.1"
                     /translation="MAFIVGLLQRHDAVFVPLSEIFQRGAPEPFVIKRFDDSRIFTPG
                     AEKIDFSRVPAFAEIQEKMAASKWGVGG"
     gene            complement(1683..>2392)
                     /locus_tag="VIS74_04330"
     CDS             complement(1683..>2392)
                     /locus_tag="VIS74_04330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792500.1"
                     /translation="NAAANKPGGQKHRPQPVTAAPNVAGKPGGAKKSGENNQASNVGG
                     NREPNAAANKPGGQKHRPQPVTAAPNGADKPGGAKKSGENNQASNGGGNRVSGGQKHR
                     PQPVTAAPNVAGKPGGVKKSGENNQASNISGNRKPDAGKPKHSPSAAPLPQERPSSVE
                     QRPKQTQERPSSVQQRPKQTQERPSSVQQRPKQTQERPSSVQQRPKQTQERPSQAQQR
                     PSGGNKQGDAKKKDKSD"
BASE COUNT          430 a          693 c          739 g          530 t
ORIGIN      
        1 ggacgggctg atcctgcgtg agccttggtt ggttcgactt attttcgcga ggcgatatgc
       61 ggagtcatgt gaggcctccc gatccgcctg aggaaaaaca tcatgtcatt ccaggcttat
      121 ctcaccaata ttcaggcaaa gacgggcaaa agccccgcgg attttcgggc gcttgccgag
      181 aaaaaaggat tcacccagaa cggcaaactt gccgccggcg tgaaagcggg agccattgtc
      241 caatggctaa aggacgactt tcaactgggc cacgggcacg cgatggccat ctacgccttg
      301 ctcaaaggag tgaaacgcga agagagccct tgagccggtt cgccgggaag cgtccggcgc
      361 atgggcacgc ggtgatcaaa ccgtggaaat cggggtagat caatcgtggg attgacgggt
      421 agggcgcgct ggctcagcgc gcctggccag gcgtcctcac gcgccacaga aagcgacatg
      481 cgcgatgttc catttttcaa tagcgaaatt ggggtgacgc ataaggcctg catcattcct
      541 gacaggttgc cgacaaaccg caccagcacc cacacgagat gagtgacgtg tcgcaaacct
      601 tcgcttcacg acttcttcac gaagcaggct ttcaggccga tggcgccgcc gtcgatcttg
      661 caggaaatct cgtgatcgcc atccaccaga cgaatgcctt tcgccttcgt gccgcctttg
      721 atgacctggg acgacccctt caatttcaaa tccttgatca gcgtcaccga atcgccgtcc
      781 gccagcaccg tgccgaaggc gtccttgacg acgcgggccg cttccggctg ggactcgcat
      841 ttccactcgt gaccgcaggt ggcgcattca cagcgatcgg ggtgtttcaa cacatcggtc
      901 atttcgcaca tcggacaggc gggattgctc ataaggaggc gaaaccgtaa cccggtttcc
      961 cgccgcggct caatcgccgt ttgttcagga atgctccccg ttcaactgcc cggcaagcgc
     1021 gggcgggacc accgaacaag gatcgcggaa aaagccgcgc tggcgaaggg atggcgcaac
     1081 cgagcggttt gtgaaaaaag cgccaagctt tacacaaagc atcactgtag cgtcggtcta
     1141 tgaccgccgc acttttttcc tcagcatttt aatcccggcg ctcatagagc gccgcttaca
     1201 gacacatgta tgccctcgcc cccctgcggg cgctcttggc cgcgaggctg aaggagtttg
     1261 ttgagaaaag catggaggtt tacgcgaaga cgtagaacca ctttttcggc gagattgacc
     1321 ctgaaaagga cgggacgttt gctttcaaga gcccgaagat atggccttca ttgttggtct
     1381 tctccaaaga catgatgcgg tctttgtgcc tctttcagaa atctttcaac gcggcgctcc
     1441 tgagcctttt gttatcaaac gctttgacga ttctcgaatt ttcacacccg gcgccgagaa
     1501 gatcgacttc tctcgcgtgc cggcatttgc cgagattcag gaaaagatgg ccgcttccaa
     1561 gtggggcgtt ggaggctgag accagatggg cgcggaggtt taggagtcgc ggaatcgaag
     1621 tgaaaagggg acaggcgacc cgaagggctt tgttcgagga gagcgggagt gcttaaggga
     1681 gcttaatcac tcttatcctt tttcttggcg tcgccctgtt tattgccgcc gctcggacgc
     1741 tgctgtgctt ggctcggacg ctcctgggtc tgcttcggac gttgctgcac cgaactcgga
     1801 cgctcctggg tctgcttcgg acgttgctgc accgaactcg gacgctcctg agtctgcttc
     1861 gggcgctgct gcaccgagct cgggcgctcc tgggtctgct tcgggcgctg ctccaccgag
     1921 ctcggacgct cctggggcag tggtgcggcg gacggactgt gcttcggctt tccggcatcg
     1981 ggctttcggt tgccgctgat gttggaagct tggttgtttt caccggactt tttaacgccg
     2041 ccgggtttgc ccgccacgtt cggagcggcg gtcaccggct gcggacgatg cttctggccg
     2101 ccggacacgc ggttgccccc gccgttggaa gcttggttgt tttcaccgga ctttttggcg
     2161 ccgccgggtt tgtccgcccc gttcggggcg gcggttaccg gctgcgggcg atgcttctgg
     2221 ccgccgggct tgttcgccgc ggcgttgggc tcgcgattgc cgccgacgtt ggaagcttgg
     2281 ttgttttcac cggacttttt agcgccgccg ggtttgcccg ccacgttcgg ggcggcggtt
     2341 accggctgcg ggcgatgctt ctggccgccg ggcttgttcg ccgcggcgtt gg
//
LOCUS       DATDAY010000220         3701 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_1846042, whole genome shotgun sequence.
ACCESSION   DATDAY010000220 DATDAY010000000
VERSION     DATDAY010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 3701)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3701)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3701
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1846042"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..833
                     /gene="trpD"
                     /locus_tag="VIS74_04335"
     CDS             <1..833
                     /gene="trpD"
                     /locus_tag="VIS74_04335"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR01245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HEY5792501.1"
                     /translation="PFTLDSPRPLLDVCGTGGDQAGLFNVSTTVLFVAAACGAGIVKH
                     GNRGITSKSGGADALEALGIPIDLSAEAAAGFFRKNGFVFLFAPAYHPAFRTVAPVRK
                     HLSEQGKRSVFNILGPLLNPARPDFQLAGVFHPRLLPIYTEVFRLLGRKTAWTVSGAL
                     PGGGWLDELSTLGKSPVRQLREGVVTDFEIDPLALGLRPATLAELQGGHAAKNAYMIE
                     EILCGENRTACRDIVLLNAAGALNAAGLAADLPEGITRAAEALDSGAAWGVLEKARKS
                     "
     gene            1054..1467
                     /locus_tag="VIS74_04340"
     CDS             1054..1467
                     /locus_tag="VIS74_04340"
                     /inference="COORDINATES: protein motif:HMM:NF020360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase substrate binding
                     protein"
                     /protein_id="HEY5792502.1"
                     /translation="MNLDIRWKQRFQNFDHAFVLLRQALEHGPAALNPLEKEGVIQRF
                     EYSFELAWKTVKDYLEQNGIILATVAPRPVLKETFAARVIADGQVWIDMLDHRNLLAH
                     TYNGAVFDEAVEAIHARYLPAMAALHEFLRHESPK"
     gene            1503..1781
                     /locus_tag="VIS74_04345"
     CDS             1503..1781
                     /locus_tag="VIS74_04345"
                     /inference="COORDINATES: protein motif:HMM:NF037100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HEY5792503.1"
                     /translation="MTSVFRRHPEITSAILFGSRAKGTHTARSDVDLAISGTVDPLRA
                     EALAAELDELPLPYRFEVQSFAHITHRPLREHIERVGIPVYPQAAIPA"
     gene            2025..2156
                     /locus_tag="VIS74_04350"
     CDS             2025..2156
                     /locus_tag="VIS74_04350"
                     /inference="COORDINATES: protein motif:HMM:NF016167.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HEY5792504.1"
                     /translation="MAQTLLDYLNAGDSLEDFLEDFPSVNPDDAAYSLRLAREESPS"
     gene            2137..2316
                     /locus_tag="VIS74_04355"
     CDS             2137..2316
                     /locus_tag="VIS74_04355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792505.1"
                     /translation="MKKVLLDENLPIGLRDHLAEFEVVTVHYQGWSGIQNGELIERID
                     GPYAVLLRNAKEEDF"
     gene            complement(2568..3473)
                     /locus_tag="VIS74_04360"
     CDS             complement(2568..3473)
                     /locus_tag="VIS74_04360"
                     /inference="COORDINATES: protein motif:HMM:NF017987.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChuX/HutX family heme-like substrate-binding
                     protein"
                     /protein_id="HEY5792506.1"
                     /translation="MNSEDKPRPAHSASDDLTQKLLADIDPANLRCGDGHYEIQLGTE
                     WIDMLATLPQIGEALVITGNPCAILGQTRRYPEIDFCPGGRHARSLDGHFDFEFVPWS
                     AARLIRRQYRNKVSHLIEFRDESNATFHKIGRTDQTDPARFVEWVQDHQASGRRAASD
                     SPVPPARWRTVRQRHWFDYDEIEPAGSRALLRVLEAAQRQERPLTAVVGNEGITQSAT
                     FIPQSLIPLDSWIFASDDVAAFHFDLEALGETLIHHLPPPPGHSPFSSLRSFDESGNL
                     RCALLPPPGTPLSEWTEFLREAAVE"
     gene            3441..>3701
                     /locus_tag="VIS74_04365"
     CDS             3441..>3701
                     /locus_tag="VIS74_04365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792507.1"
                     /translation="MGGPGFVLGIHQAANLTHAADPLLSSGCEMLAADCLFYRKERKE
                     LREGRSFFNRDPRLNSGFWSSLSQSFFAGFFFELSAFLAVKKS"
BASE COUNT          698 a         1101 c         1118 g          784 t
ORIGIN      
        1 tgccgttcac cctggactcg ccgcgccccc tgctcgacgt ctgcggcacc ggcggcgatc
       61 aggcggggct ctttaatgtg tccaccaccg tgctcttcgt cgccgccgcc tgcggggcgg
      121 gaatcgtcaa gcacgggaat cgcggcatca cctccaaatc cggcggcgcg gacgcgctcg
      181 aagccctggg cattcccatt gatctctccg ccgaagccgc cgcgggtttc ttccgcaaaa
      241 acggatttgt gttcctcttc gccccggcgt atcaccccgc gtttcgaacc gtcgcgcccg
      301 tgcggaagca cctgagcgag caaggcaaac gcagcgtttt caacatcctc ggcccgctgc
      361 tcaaccccgc ccgcccggat tttcagttgg ccggagtttt tcatccccgc ctgctgccca
      421 tttacaccga ggtcttccgc ctgctcggcc ggaaaaccgc ctggaccgtc agcggcgccc
      481 tgcccggagg gggatggctc gacgagctct ccaccctcgg aaaatccccg gtccggcaac
      541 tccgcgaggg cgtggtgacc gacttcgaaa tcgacccctt ggccctcggc ctgcgccccg
      601 ccacccttgc cgaactacag ggcggccacg ccgcgaagaa cgcctacatg atcgaggaaa
      661 tcctctgcgg cgaaaatcgc accgcctgcc gcgacatcgt cctgctcaac gccgccggcg
      721 ccctcaacgc cgccggcctg gccgcggacc tgcccgaggg catcacccgc gctgccgaag
      781 ccctcgactc cggcgccgcc tggggcgtcc tggaaaaagc ccggaagtcg tagcttgatt
      841 caaaaacggg ttcacgcgga gacgcgaagc cgcggaggat cgcaagaaaa gtcggaaggc
      901 aggatcaaac gccaaaaatg ctggtcttta ctttgaaacc aatgctcatc cctttgtgtc
      961 ctttgtgatc ttctgttcaa atcccttcgc gtcctccgcg aggccggttg gaaaaagcta
     1021 tccgggcaaa tctgcacgcg ttaaattccc cgcatgaatc tcgatatccg ctggaagcaa
     1081 cgtttccaga acttcgacca tgcctttgtc ctgctgcgtc aggcattgga gcatgggccg
     1141 gcggccttga acccgctgga aaaggagggc gtcatccagc gttttgagta ttccttcgaa
     1201 ctggcttgga agaccgtaaa ggattacctg gagcagaacg ggatcattct tgccacggtg
     1261 gcgccgaggc cggttttgaa ggaaaccttt gccgccaggg tcattgccga tggccaggtc
     1321 tggattgaca tgctcgacca ccgcaacctg ctcgcccata cctacaatgg agcggtgttt
     1381 gacgaggcgg ttgaggccat ccatgcccgt taccttcccg ccatggcggc gctgcatgag
     1441 ttcctgcgcc acgagagccc caaatgactc cgccgccgct cacgcttcac gaattatccc
     1501 tgatgacatc cgtctttcgg cgtcatccgg agattacctc ggccattttg tttggctcgc
     1561 gggcgaaggg cactcacacc gcgcggtcgg atgtggacct cgccatctcc ggcaccgtgg
     1621 accctctgcg ggccgaggcc ctcgcggcgg aactggatga attgcccttg ccctatcgtt
     1681 tcgaggtcca atccttcgct cacattaccc atcgtccgct gcgcgagcac atcgagcggg
     1741 tgggcatccc ggtttatccc caggcggcca tccctgcatg aaagacaagg gaaaagatgc
     1801 ctcacgcgga gacgcgaagc cgcggaggat cacaagaaaa gtcggaaggc aggatcgaat
     1861 gcccaaaatg ccggcagact tcgagtagat gggggcgatg cggaaaggat tgccgccgcc
     1921 ttttcgtttg ctatcttaaa gtcatggtca cgctcgatca tagtccggtt cattccgatc
     1981 cgcggattct tggaggcacg ttggttttcc ggaacacacg ggtaatggcg caaaccctcc
     2041 tggattactt gaatgcgggc gattccctgg aggatttttt ggaggatttt ccctcggtga
     2101 atccggacga tgcggcctat tctttaaggc tcgctcgtga agaaagtcct tcttgacgaa
     2161 aatctgccta tcggattgcg ggatcatctt gccgaattcg aagttgtcac cgtccactat
     2221 caaggttggt cgggcattca aaatggcgag ttgatcgagc gaattgatgg cccgtacgcg
     2281 gtccttctta gaaacgcgaa agaagaagat ttttgaacag aaggaagcaa agcaagcaaa
     2341 ggcttgggat ttgctccttc gtttcctttg ctttcttctg ttaaatacgt ccggcggtga
     2401 cggcgcaccg ccctccaaaa taatcaaaaa gggagccgtg ctttctctat ctaacgcggc
     2461 ctttcttatc cgattcttcg cggcttggcg ccttggcgtg agaaattgcc ggcctttttg
     2521 tttttcttcg ggctttttag agacttcggg ggaggcttgc cgcagcttca ttcaaccgcg
     2581 gcttcccgca aaaattccgt ccattcgctg agcggggtgc cgggcggggg gaggagggcg
     2641 cagcggaggt tgccggattc gtcgaagctc cttaacgagg aaaacgggga atggccgggc
     2701 ggcgggggga ggtgatggat gagcgtttcg ccgagcgctt cgagatcgaa atggaacgcg
     2761 gcgacgtcgt cactcgcgaa gatccagctg tccagcggaa tgaggctttg cgggatgaag
     2821 gtggcgcttt gggtgatgcc ttcgttgccg acaacggcgg tgagcgggcg ctcctggcgt
     2881 tgggcggctt ccagcacgcg cagcagagcc cgggagccgg cgggctcgat ctcatcgtag
     2941 tcgaaccaat ggcgttgccg caccgtgcgc cagcgggcgg ggggaaccgg ggaatcactc
     3001 gcggcgcggc gtcccgaggc ttgatggtcc tgcacccatt ccacgaaccg ggcggggtcg
     3061 gtttggtcgg tgcggccgat cttatgaaag gtggcgttgg attcgtcgcg aaattcgatg
     3121 aggtgggaaa ccttgttccg gtattgccgc cggatcaggc gggcggcgct ccagggcacg
     3181 aattcgaagt cgaaatgccc atcgaggctc cgcgcatgcc ggccgccggg gcaaaagtcg
     3241 atttcgggat aacggcgcgt ctggccgaga atggcgcagg gatttccggt gatgaccagc
     3301 gcctcgccga tttgcggaag ggttgccagc atgtctatcc actccgtgcc gagctggatt
     3361 tcataatggc cgtcgccgca gcgaaggttg gcgggatcaa tgtcggcgag cagcttttgg
     3421 gtgagatcgt ccgacgccga atgggcgggc cggggtttgt cctcggaatt catcaggcgg
     3481 ccaatctaac ccatgccgcc gatcccctgc tttcctcggg ttgcgaaatg ttggcggcgg
     3541 attgtttatt ttaccgcaaa gaacgcaagg aactcagaga gggacggagt ttttttaacc
     3601 gtgatccgcg gttaaattcc ggcttttgga gcagcctctc acagagtttc tttgccggct
     3661 tcttctttga gctctctgcg ttccttgcgg ttaaaaaatc c
//
LOCUS       DATDAY010000221         3635 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_551882, whole genome shotgun sequence.
ACCESSION   DATDAY010000221 DATDAY010000000
VERSION     DATDAY010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 3635)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3635)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3635
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_551882"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            294..623
                     /gene="trxA"
                     /locus_tag="VIS74_04370"
     CDS             294..623
                     /gene="trxA"
                     /locus_tag="VIS74_04370"
                     /inference="COORDINATES: protein motif:HMM:NF012314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEY5792508.1"
                     /translation="MNSSEPLHVNEASFERAVTQSPTPVLVDFWAPWCGPCKMISPVL
                     DEIAREKAETLRVVKVNVDDNPALAQKFNVRSIPTLLVFAGGQLKETIAGAASKQTLL
                     TKIAAAA"
     gene            657..1415
                     /locus_tag="VIS74_04375"
     CDS             657..1415
                     /locus_tag="VIS74_04375"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HEY5792509.1"
                     /translation="MSTAQKLNGKTAIVTGASKGIGAAIAKHLAAAGASVAVNYASSK
                     EGADRVVAEITAKGGRAAAIQADVSKPADIQRLFEKSAAAFGPLDILVNNAGIFEFAP
                     LEAVTAEHFHRQFNLNVLGLILASQEAANRFGPNGGSIINISSGAGQSPVAGASVYSA
                     TKAAVDAITSSLSKELGPKKIRVNSLSPGMVETEGVHAAGFLGTDFHKAIIAQTPLGR
                     IGQPDDIGPVAVFLASEDSGWISGEKLLVAGGQR"
     gene            1635..2003
                     /locus_tag="VIS74_04380"
     CDS             1635..2003
                     /locus_tag="VIS74_04380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792510.1"
                     /translation="MTLIGSAGLLWHAFQGGIEEPFPENSMDADPISDDEEAVPLAYD
                     AMIGSEFELTHGWHINLMPRAVLRELPESWAARASKKNYRSLRVIVPTAADLLAPKLR
                     RGEPRDVAHAEWSRRIGIAV"
     gene            1966..2310
                     /locus_tag="VIS74_04385"
     CDS             1966..2310
                     /locus_tag="VIS74_04385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792511.1"
                     /translation="MQSGAVASELLFDRPEFKYFPPEDFARLDYGRLLWKAPAGRARL
                     LAHWNDPRHPYRERFLRHRPLLESLLSCALDRLDDAAVEHGQSLRAAIREIPPVFGHF
                     WADSLRENRPVA"
     gene            2391..3005
                     /locus_tag="VIS74_04390"
     CDS             2391..3005
                     /locus_tag="VIS74_04390"
                     /inference="COORDINATES: protein motif:HMM:NF028235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEY5792512.1"
                     /translation="MPRISTAKERLTDAALEMIWENSYGATSVDAICERANVRKGSFY
                     YFFKSKSELAAAALEADWKKHQADLDAIFSPTVPPLERFQRHFEHGYAFMTGIKEKYG
                     AVLGCPVFTLGCEISTLDSVLRDKIQELLNRKLAYLESAIRDAHARGEVDAPDAQSKA
                     RAVLAFVEGSLSQARIENSLEPIRQLYAGTLALLGAKPLQLTAV"
     gene            3072..>3635
                     /locus_tag="VIS74_04395"
     CDS             3072..>3635
                     /locus_tag="VIS74_04395"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEY5792513.1"
                     /translation="MKTTIRRVPLQILALSLLAFSLGGCGKGKSPMQAPPPPAVTVAP
                     VIQENLIEWSEFTGRTDAVESVEVRPRVSGHIEEVRFQSGQLVKKGDVLFVIDPRPYQ
                     AESDQRTAEFEQAKARYENAGRIAQRAAKLLERKTIADEAADTSLSEFQEAKAALLAA
                     QAAMESARLDLGFTQVRAPIDGRVSRAL"
BASE COUNT          763 a         1157 c         1047 g          668 t
ORIGIN      
        1 ctggagcatt acctgcccag accgcagaaa accgaagccg aagtgctcgc ggcaatggcg
       61 caacgccacc gcgcaagaat ggccgcgtcg gcctccgcgc aaaaaagaaa ctcgacccag
      121 aaattaacaa agtagaatta accgcaaaga acgcaaagag cgcagagagt tgatggcaaa
      181 tgtggcttgc tcggaccact cggcttcatt cgatgaccac cccatcttat ccgctttttt
      241 gcggtctccg cgttctttgc ggttaaaaaa aattcatcca actcaaaaaa accatgaact
      301 catccgaacc actccatgtc aacgaagcca gcttcgaacg cgccgtcacg caatcgccca
      361 ctcccgtcct cgtggacttt tgggcgccct ggtgcgggcc gtgcaaaatg atcagccccg
      421 tcctcgacga aatcgcccgc gagaaggcgg aaacgctccg cgtggtgaaa gtcaacgtgg
      481 atgacaatcc cgcgctggcg cagaaattca acgtgcgttc catccccacc ctgctcgtct
      541 tcgcgggcgg ccagttgaag gagacgatcg ccggcgccgc aagcaagcag acgctgctga
      601 ccaagatcgc ggccgccgcc tgaattttcc caaaacccaa tccaaagaaa catcttatga
      661 gcacagccca aaaactgaac ggaaaaaccg ccatcgtcac cggagcctcc aagggcatcg
      721 gcgcggcgat cgccaaacac ctcgcggccg ccggcgcttc cgtcgcggtc aactatgctt
      781 ccagcaagga aggcgccgat cgcgtcgtcg cggaaatcac cgccaagggc gggcgggcgg
      841 ccgccattca agccgacgtg tccaaaccag cggacatcca gcggcttttc gagaaatcgg
      901 ccgccgcctt cggcccgctc gacattctcg tcaacaatgc cggcattttc gagttcgccc
      961 cactggaagc cgtcaccgcc gagcacttcc accggcaatt caatttgaac gtgctcgggc
     1021 tcattcttgc gagccaggaa gcggccaacc gcttcggacc gaatggcgga agcatcatca
     1081 acatcagctc cggcgcgggg cagtctccgg tcgccggcgc ttcggtttac agcgccacca
     1141 aggcggcggt ggacgccatt acctcctcgc tctccaagga actcgggccg aaaaaaatcc
     1201 gcgtgaattc cttaagcccg ggaatggtgg aaaccgaagg cgtgcatgcc gcgggctttc
     1261 tgggaacgga cttccacaag gccatcatcg cccagactcc gctcggccgc atcggccagc
     1321 cggatgatat cggcccggtc gcggtgtttc tcgcctcgga ggattcggga tggatttccg
     1381 gcgagaagct cctggtcgcg ggaggacagc gttgagccat tcgtccgcaa gcccgcatcg
     1441 gccacgttca cctgaaggtg gccgatctgg ctggacgccc cgtttccatg ggcatccatg
     1501 actgcgccgg gatctttttg cggattgctc caccggcaga aagggagtaa aacgcccaaa
     1561 cgtgaacttc ggactcccgt ccaggtggaa aactgtctga agggctgggg aaacacggta
     1621 acctcccttg ttccttgacg ttgatcggtt cggccgggtt gctctggcat gcttttcaag
     1681 gggggattga agaaccattt ccggaaaaca gcatggatgc cgatccgatc tccgatgacg
     1741 aggaagccgt cccgctggct tacgatgcca tgattggcag cgagtttgag ctgactcacg
     1801 gatggcatat caatctgatg ccccgggccg tcctccgtga gttgcccgag agttgggccg
     1861 cccgcgcttc taaaaaaaat tatcgttctc ttcgggtaat cgttccaacc gctgcggatt
     1921 tgcttgcgcc aaagctgcgg cggggcgaac ctcgggacgt cgcgcatgca gagtggagcc
     1981 gtcgcatcgg aattgctgtt tgaccggccg gagtttaagt atttcccgcc ggaggatttt
     2041 gcccgcctgg actatggccg gctgctctgg aaagcgccgg ctggccgcgc ccgtttgctg
     2101 gcgcactgga atgatccccg gcacccctac cgcgaacgat ttcttcgcca ccgtccgctg
     2161 ctcgaatccc tgctgtcctg cgctttggat cgcctggatg acgctgcggt cgagcatggg
     2221 caaagccttc gcgccgccat ccgggagata ccgcccgtct tcggtcattt ctgggcggat
     2281 tccctccggg aaaaccgtcc ggtggcctga cagcgccttc ctttttgagg cagcctggcg
     2341 aaaacggaat aatagttgac tatttagtca atatcgcgga aggtagtttt atgcctcgca
     2401 tcagcaccgc gaaagaacgt ctgactgacg ccgccctgga gatgatctgg gagaacagtt
     2461 atggcgcgac gtcggtggat gccatttgcg aaagggccaa cgtccggaag ggcagctttt
     2521 attatttctt caaatcgaag tccgaactgg cggcggcggc gctggaggcg gattggaaaa
     2581 agcaccaagc cgacctggac gcgatttttt ccccgaccgt gccgcccctg gagcgctttc
     2641 aacggcactt cgagcatggt tatgccttca tgacgggcat caaggaaaaa tatggcgccg
     2701 ttctgggttg tccggtgttc acgctgggct gcgaaatcag caccctcgat tcggtgttgc
     2761 gcgacaagat ccaggaactg ctcaaccgca agctggccta cctcgaatcc gccatccgcg
     2821 acgcccacgc ccggggcgag gtggacgctc ccgacgccca aagcaaggcg cgcgcggtgc
     2881 tcgccttcgt cgaaggctcc ctgtcccagg cccgcatcga aaacagcctc gaacccatcc
     2941 gccagcttta cgccggcacc cttgccctgc ttggcgccaa accattgcaa ctcactgcgg
     3001 tctaaatttt tttgccgatt agatagacga ctagtcaaat accaaacctt aaacgcgaca
     3061 tccaaaaaaa catgaaaacg accatccgcc gcgttcccct ccagatcctg gccctcagcc
     3121 tgcttgcctt cagcctgggc ggctgcggca agggaaagag cccgatgcag gccccgccgc
     3181 cgcccgccgt cacggtggcg cccgtcattc aggaaaacct gatcgaatgg agtgaattca
     3241 ccgggcgcac cgacgcggtg gagtccgtcg aggtccgccc ccgcgtctcc gggcacatcg
     3301 aggaagtgcg tttccaatcc ggccagctgg tcaaaaaggg cgacgtgctt ttcgtgatcg
     3361 atccgcgccc ctaccaggcg gagtccgatc agcgcacggc ggaattcgag caggccaagg
     3421 cgcgttacga aaacgcgggc cggatcgccc agcgggccgc caagctgctg gagcgcaaga
     3481 cgatcgccga cgaggcggcg gatacgagcc tctccgaatt ccaggaagcc aaggccgcgc
     3541 tgctggcggc gcaggcggcg atggaatccg cgcggctcga tctcggattc acccaggtgc
     3601 gcgcgccgat tgacggacgc gtcagccgcg ccctg
//
LOCUS       DATDAY010000222         2537 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_143086, whole genome shotgun sequence.
ACCESSION   DATDAY010000222 DATDAY010000000
VERSION     DATDAY010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2537)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2537)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2537
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_143086"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            146..1243
                     /gene="murG"
                     /locus_tag="VIS74_04400"
     CDS             146..1243
                     /gene="murG"
                     /locus_tag="VIS74_04400"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:TIGR01133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HEY5792514.1"
                     /translation="MPKSLRVVIACGGTGGHLFPGLAVAEELHGRGHEILLIVSEKAI
                     DSIALKSHPEFRAERLPSVGMPSVLSPAFIRFLRLSWESISMCRHIYKKFQPDRVLGM
                     GGFTSTPPILAARRQGIPSFIHESNAIAGKANRLSARFATKVLIGFEETARFFANSDC
                     IFTGTPVRRNLGQRIPRDEAITRFKLDPARKTLLVMGGSQGASGINQLMFRAAHSLRD
                     RPVQIIHFTGDRDDQLAAANYLREGVPAYVAAFHHQMEEAYSAADLLITRAGASSLSE
                     ISAFGLPSVLVPYPFAADDHQKANALIFARRGAAEIVTESETQAESFAILLQNLLQDD
                     ARRRKMAKEAARTQPRDAAVRVADVVESVTS"
     gene            1240..>2537
                     /gene="murC"
                     /locus_tag="VIS74_04405"
     CDS             1240..>2537
                     /gene="murC"
                     /locus_tag="VIS74_04405"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEY5792515.1"
                     /translation="MNPDELAQLLHGAPTRIHLIGVAGSGMSGIAALLLALGHQVSGS
                     DRSETGEVQRLVRRGLKFSTPHHASQVRDAQAVIYSSAIRSGNPAYDEATRLCLPMFR
                     RADALAALMTGKKGVVVCGMHGKTTTSAMAAYVLRASGLKPSHYVGAEIPILGSNARW
                     DSEGELFVAEGDESDGTLVNYHPEHAIVLNIEPEHLDFYKDLAAIDAVYSRLTAQTTG
                     WIFYCGDDEGATRVCGRLPNAISYGTRPGAAYRLADLSLRQFRPHFSVYRNEARLGEV
                     HLGVPGEHNAVNSLAVIALAHEMGGEFSIIANALERFRGARRRFQVKYDDDYNVVVDD
                     YGHHPTEIAATLATARSGGAKRIRVMFQPHRYSRTAALREEFGACFAEADSVVVSEIY
                     AASEKPIPGISGQTIVDSLLAHGHSQARFEPNFDRIHKVVG"
BASE COUNT          430 a          880 c          788 g          439 t
ORIGIN      
        1 catgcccctt ccgtttatca gttacggcgg atcgaatctg ctcgtctgcc tctttttggt
       61 cggcctgctc gtgagcattc accggcaggg aattcccgta ttcgagaaac cgccgaggcc
      121 gcgcctgacg ccgcgggtat aagccatgcc aaaatccctg cgagttgtca ttgcctgtgg
      181 aggaaccggc ggacatttgt tccccggcct ggccgtcgcc gaagaattgc acgggcgcgg
      241 gcacgagatt ttgctgatcg tctcggagaa ggcgattgat tcaatcgcgc tcaagtcgca
      301 tcccgagttc cgcgcggagc ggcttccctc cgtcggcatg ccctccgtgc tttcgcccgc
      361 gttcatccgc tttctgcggc tgagctggga gagcatcagc atgtgccgcc acatctacaa
      421 aaaattccag cccgaccgcg tgctgggcat gggcggcttc accagcacgc ccccgatcct
      481 cgcggcgcgg cggcagggaa tcccctcctt catccacgaa tccaacgcga tcgccggcaa
      541 ggccaaccgg ctctccgccc gcttcgcgac caaggtgctg atcggcttcg aggaaacggc
      601 gcgcttcttt gccaatagcg actgcatttt caccggcacg ccggtccgcc ggaatctcgg
      661 gcagcggatt ccccgggatg aggcgatcac ccggttcaag ctcgatcccg cgcgcaagac
      721 gctgctcgtc atgggcggca gccagggcgc ttccggcatc aaccagctca tgttccgcgc
      781 cgcgcattcg ctgcgggacc gcccggtgca gatcattcat ttcaccggcg accgcgacga
      841 ccagctcgcc gccgcgaatt acctccgcga gggcgtcccc gcctacgtcg ccgcgtttca
      901 ccatcaaatg gaggaagcct actccgccgc ggatctcctg atcacccggg cgggcgcctc
      961 gagcctgagc gagatcagcg ccttcgggct gccctcggtg ctggttccct acccctttgc
     1021 cgcggacgac caccagaagg ccaatgccct gattttcgcg cggcggggag cggcggaaat
     1081 cgtcacggaa tccgaaaccc aggccgaatc cttcgcgatt ttgttgcaaa acctgctcca
     1141 ggacgacgcc cgccgccgga aaatggccaa ggaagccgcc cgcacccagc cccgggacgc
     1201 cgcggtgcgc gtggccgatg tcgtggaatc cgtcacctca tgaacccgga cgaactcgcc
     1261 caactgctgc acggagcgcc gacccggatt catttgatcg gggtggccgg ctccggcatg
     1321 agcggcatcg ccgccctgct gctcgcgctt ggacaccagg tcagcggatc ggaccgcagc
     1381 gagaccggcg aagtgcagcg cctcgtccgg cgcggcctga aattttccac cccgcatcat
     1441 gcctcgcagg tgcgcgacgc gcaggcggtc atctattcct ccgccatccg ttcgggcaat
     1501 cccgcctacg acgaggccac gcgcctttgc ctgcccatgt tccggcgggc ggacgccctg
     1561 gcggcattga tgaccggaaa aaagggcgtg gtcgtctgcg gcatgcacgg caagaccacc
     1621 acctcggcca tggcggccta cgtgctgcgc gcctcggggc tgaagccctc ccactatgtc
     1681 ggcgcggaaa tcccgatcct cggcagcaac gcgcgctggg attcggaagg cgaactcttc
     1741 gtggccgaag gcgacgagag cgacggcacg ctggtgaatt accatcccga gcacgcgatc
     1801 gtgctcaaca tcgagccgga gcacctggac ttttacaagg atctcgcggc gatcgacgcg
     1861 gtttattccc ggctgacggc gcagaccacg gggtggattt tttattgcgg cgacgacgaa
     1921 ggcgcgacgc gcgtctgcgg ccgcctgccg aacgccattt cctacggcac gcggccgggg
     1981 gcggcctacc ggctcgcgga cttgtcgctg cgccagttcc gcccgcactt ctcggtgtac
     2041 cgaaatgaag cgcggctcgg cgaggtgcac ctcggcgttc cgggcgagca caacgcggtg
     2101 aattcgctgg ccgtcatcgc cctggcccac gaaatgggcg gggagttttc catcatcgcc
     2161 aacgcgctcg agcgcttccg cggcgcgcgg cggcgcttcc aggtgaagta tgacgacgat
     2221 tacaacgtcg tcgtggacga ctacgggcac caccccaccg aaatcgccgc cacgctggcg
     2281 accgcgcgtt cgggcggcgc caagcggatt cgcgtgatgt ttcagccgca ccgttattcc
     2341 cgcaccgcgg cgctgcggga ggaattcggc gcgtgtttcg ccgaggcgga cagcgtggtg
     2401 gtcagcgaaa tttacgcggc cagcgagaag ccgattcccg gcatcagcgg gcagaccatc
     2461 gtggacagcc tcctcgccca cggccattcg caggcgcgct tcgaaccgaa tttcgaccgg
     2521 attcacaagg tcgtcgg
//
LOCUS       DATDAY010000223         2428 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_543740, whole genome shotgun sequence.
ACCESSION   DATDAY010000223 DATDAY010000000
VERSION     DATDAY010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2428)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2428)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2428
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_543740"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            171..2072
                     /locus_tag="VIS74_04410"
     CDS             171..2072
                     /locus_tag="VIS74_04410"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY5792516.1"
                     /translation="MVVSRSIPAAPVARHTALRDGLLTGAAIFISSLLSIALLAYTAW
                     QFQDAAIRKNLSRLAQAAANLVDGDLHEKIAASGVMNSADYQFALAPLVKFHKAVPEI
                     AYLYTLIERDGKLRFGLDTAAQAQELGFDWEMEVSPLLSEYQSDSPEEDAAELAAIRE
                     GRTHVSEALYSDAFGTFLTAVAPVQNSRGQTVAVLGVDLNVKDYLTQMRDIQIAGWIS
                     IAAAALMAALLGVLVYQIRQKAQRDQDEKLRAFFEKDELEAQDQGLVRALGQAVYHHH
                     VIPDEIFWTGEYEKLLGKLPDDKCTLREWLNFVHPDDRGRVTAEYEEAIRQKGLFLSE
                     YRWIGDAASPPIWVEDRGVIARDAEGNAIRTDGVMLDISARKRAETELIAAKEKAETG
                     DRIKSEFLAMMSHEIRTPMNGVIGFTNLLLDTPLTADQRDYLESVQVCGESLLRLLND
                     ILDFSKMESGKLELEKVPFSLRKTGEEVIHLYARLSTAKPLTLHCEFSPDAPDWIRGD
                     PSRLRQILLNLIGNAVKFTEKGSVRLRVEKIFHPQGPACRISVEDTGIGISPEQMERL
                     FKPFSQADSSTTRRYGGTGLGLAICQRLVGAMGGALRAESVPGQGSRFFFDLPLEAVS
                     GGPGGSPVR"
     gene            complement(2058..>2428)
                     /locus_tag="VIS74_04415"
     CDS             complement(2058..>2428)
                     /locus_tag="VIS74_04415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792517.1"
                     /translation="ELPARYPDQIFYGKIPGGLAVLMSLDYLWTGHYPKHHRPLVECS
                     PLARKIHGLIALEPLTTMQLRRELAVETGPAKAAFAKALLELQVTLNLARRNSLQDKK
                     DVWVNFREQHLPPAAGSTDR"
BASE COUNT          488 a          764 c          723 g          453 t
ORIGIN      
        1 cgttcgatgg cggcaatctc ttgaaggatg tggtgaggct gaagatttga gttcatgccg
       61 caagtatata ctaacacata tactccgcga caaaaaagag agggggaaaa aacgtcccga
      121 taagaatttt gtcatgcacc cgcggcggga aaagccgctt aaggtgcctc atggtcgttt
      181 cccgatcaat cccggccgct ccggttgccc gtcacactgc gctgcgcgac ggactcttaa
      241 ccggcgccgc catttttatt tcctccctgt tgagcatcgc gctgctggct tacaccgcct
      301 ggcaattcca ggatgcggcc atccggaaaa atctctcccg cctggcccag gcggccgcca
      361 acctcgtgga cggcgatctg cacgaaaaaa tcgccgccag cggggtcatg aacagcgcgg
      421 actatcaatt cgcactggct ccgctggtca aatttcacaa ggccgttccc gagattgcct
      481 atctttacac gctcatcgaa cgggacggaa aactccgctt cggactggac accgccgccc
      541 aggcgcagga gctgggtttc gattgggaaa tggaggtctc cccccttctg agcgaatacc
      601 agagcgattc ccccgaggaa gacgccgccg aactggccgc cattcgcgaa ggccgcaccc
      661 acgtgagcga ggcgctctac tcggacgcgt tcggcacttt tctcaccgcc gtggcgccgg
      721 ttcaaaattc ccgcggacag accgtggcgg tgctgggggt ggatttaaat gtgaaagact
      781 acctcacgca gatgcgggac atccaaatcg cgggctggat ctcgatcgcg gcggccgcgc
      841 tcatggccgc gttgctggga gtgctggttt accaaatccg tcaaaaagcg cagcgggacc
      901 aggacgaaaa actgcgcgcc ttttttgaaa aagatgaatt ggaggcgcag gaccagggac
      961 tcgtccgcgc cctgggccag gcggtttacc accatcacgt catccccgac gagatcttct
     1021 ggaccggcga atacgaaaaa ctgctgggca aactgccgga tgacaaatgc accctccgcg
     1081 agtggctgaa tttcgtgcat ccggacgatc gtggccgcgt caccgcggaa tatgaggaag
     1141 ccatccgcca aaaaggtttg ttcctttccg aataccgctg gatcggcgac gcggcctccc
     1201 cgcccatttg ggtcgaggat cggggagtga tcgcccggga cgccgagggg aatgccatcc
     1261 gcacggatgg cgtcatgctc gacatctccg cccgcaagcg ggccgagacg gagctgatcg
     1321 cggcgaagga aaaagccgaa accggcgacc gcatcaagag cgaattcctc gccatgatga
     1381 gccacgaaat ccgcacgccg atgaatggcg tcatcggatt caccaacctg ctgctggaca
     1441 cgccgctgac cgccgaccag cgcgactacc tcgaatccgt ccaggtgtgc ggggaatccc
     1501 tgctgcgatt gctcaacgac atcctggact tttccaagat ggagtccggc aagctggaac
     1561 tcgaaaaagt ccccttctcg ctgcgcaaga ccggcgagga ggtcattcac ttgtatgccc
     1621 ggctttcgac cgcgaaaccg ctgacgctgc attgcgagtt ttccccggac gcgcccgatt
     1681 ggatccgggg ggatccgtcg cggcttcgcc aaatcctcct gaatctcatc ggcaacgcgg
     1741 tcaagttcac ggagaagggt tcggtccggc tgcgcgtgga gaaaattttc catccgcaag
     1801 gccccgcctg ccgcatttcg gtggaggata ccggcattgg catttccccg gagcagatgg
     1861 agcgcctgtt caaacccttc agccaggcgg attcctccac cacgcggcgc tatggaggca
     1921 ccgggctcgg gctcgccatt tgccagcgtt tggtgggcgc catgggaggc gcgctccggg
     1981 ccgaaagcgt ccccggccag ggctcgcgct ttttcttcga ccttcccctc gaagcggtct
     2041 ccggcggccc cggcggctca ccggtccgtt gaaccggcgg ccggcggcag gtgctgctcg
     2101 cgaaaattca cccacacgtc ctttttgtcc tgaaggctgt tgcgccgggc aagattcagg
     2161 gtcacctgta gctccaaaag cgccttggcg aatgccgcct tggccggccc cgtttccacg
     2221 gccaactccc gccgcagctg catcgtcgtc aggggctcaa gcgcgatcaa cccgtggatt
     2281 ttccgggcga gcgggctgca ttccacaagc gggcgatggt gcttcggata atgcccggtc
     2341 cacaaataat cgaggctcat cagcaccgcg aggccgccgg ggatcttgcc gtaaaaaatc
     2401 tggtcgggat accgggccgg cagttcgt
//
LOCUS       DATDAY010000224         4747 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_1514022, whole genome shotgun sequence.
ACCESSION   DATDAY010000224 DATDAY010000000
VERSION     DATDAY010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 4747)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4747)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4747
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1514022"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..207)
                     /locus_tag="VIS74_04420"
     CDS             complement(<1..207)
                     /locus_tag="VIS74_04420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HEY5792518.1"
                     /translation="MSAVWYQCQRCGNCCRWPGEVKLREPEVPAIAAYLGLSEDDFIQ
                     NHTRLQASRRGLALLDKPNGECAFL"
     gene            254..1117
                     /gene="rfbD"
                     /locus_tag="VIS74_04425"
     CDS             254..1117
                     /gene="rfbD"
                     /locus_tag="VIS74_04425"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: protein motif:HMM:NF016228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HEY5792519.1"
                     /translation="MKLAILGSNGRLGATLCQEWRERFEIQPIGRAQLDLAHEMAVRE
                     FARSIQADAVLNPAGLTSLEACEDQPGAANAINARAPELLAAGLAARDIPLLHFSTDY
                     VFDGSKSGLYTEADTARPISLYGRSKLAGEEAVLAASPRHWVLRVSWLFDGGRRAFPD
                     QILEQARTQDRIEAVADKISVPTSCRDLADWLADLLPDLSREGGLYHACSRGRCTWQE
                     YAQFILDAIPHPRCRQLTPIRLRDVPFFRAARPANSAMDSARFFRRLGRDAPDWQSAL
                     TKFLHRLTNPA"
     gene            1126..1857
                     /locus_tag="VIS74_04430"
     CDS             1126..1857
                     /locus_tag="VIS74_04430"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: protein motif:HMM:NF008692.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HEY5792520.1"
                     /translation="MHRFFVSPADLANNAFTLSPEESHHCLSVLRHQPGDRVTLFDGR
                     GNEATGILGGVEGKLAQVKIQLRSKTAPLRCRLTLAQAIPKGKNMDLVVQKAVELGAA
                     AIAPILSERTVVSLDGAEIAKKQEKWQALALEACKQCGQNFVPEVLAPQPLKRFFETK
                     AKDDLMLIASLQPDAKPVKTVLADAGKRPASVTVFVGPEGDFTPAELGLAKSSGCQPV
                     TLGPIILRTETAAIYCLSVLGHELY"
     gene            complement(1849..2979)
                     /locus_tag="VIS74_04435"
     CDS             complement(1849..2979)
                     /locus_tag="VIS74_04435"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEY5792521.1"
                     /translation="MERLLVVSEPGVDGVFACVRSLIEYLHRRHPEIRVDLAYSSRRA
                     GGALPGLVAAVERRGGETLDLRVGNSPEFRDFAAAAALLQWMRRRRIQLVHAHSSKAG
                     ALARLGALVPGFPPVLYSPNAYFGMPRLGGKKEFFFNGIESLLGRLAPTHNCSEDERD
                     FAIRVLKLNPSSLHVIHHGIDLERFSPADAETRARLRRELGLPAEARLLVTVGRASVQ
                     KNYGPLYAALDGFLPGSGAAFAHAGNGSVELRQSLSPAARSSAFAFHYLDQPEALLRA
                     ADGFILTSRYEGLSFSMLQAFGCGLPAILAEAPGLRVAKALGFSGVRWLPDPVRAADV
                     RRALEEWNRSAGAAGNSRELALHYFSESRQLEEMIGLYRRLI"
     gene            complement(2979..4073)
                     /locus_tag="VIS74_04440"
     CDS             complement(2979..4073)
                     /locus_tag="VIS74_04440"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEY5792522.1"
                     /translation="MHRVAFNGRFSGTPQPTGTQTVAFQLFDAILREPRSFEAVVFAD
                     SRFPGISEWRTLPGVKWIEVPFQDWPRAWAQFWEQFFLPRLARQHGCRLAHHPITTCP
                     VWQNGVKNVVTLHDLNFHRHPEWYSRSFRLVYALGAVPGLKRAARVVTISNYVRGQAA
                     ESFNLRPERLRMVYNGVKPLAAPGPAGGGNYLLCVGSLQPHKNLVRLIRAYLKIQPEF
                     PGLELRVVGRPQARFAAESELPALLSAPGVQLLGYLSEAGLAQAYAGARVFCYPSLEE
                     GFGLPVLEAMTLGAPVLTSNVSCLPEVAGPATLVDPYSVEAIAGGLRQLLALAPPALA
                     ALAAEGRAWAARFSWAAAARGYLKLYGELF"
     gene            complement(4054..>4747)
                     /locus_tag="VIS74_04445"
     CDS             complement(4054..>4747)
                     /locus_tag="VIS74_04445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008755 - O antigen polymerase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="O-antigen polymerase"
                     /protein_id="HEY5792523.1"
                     /translation="VTLEKKLPRPVLVAAFAASLFLFAGLSGFTAILRQDRSALDEGN
                     IVGQSLREAFLGDNLIDLRDAAWVFSEWDFEPLNGKTYLGGLTAMLPSGVFPQKKEWH
                     LGLTGVRMVGWNPDEHFGLRITFFGESFLNFGPAGVVGLAVIMGCLFGGLLRTLHLIS
                     AKRPPCLYCGLKVVVMMQMCLPLANTSDAFTFWSMGAFLVLQWLAVGLPPRLRRLNAS
                     DPSVSAHAPSRV"
BASE COUNT          929 a         1588 c         1527 g          703 t
ORIGIN      
        1 cagaaacgcg cactcgccat tgggtttgtc gagcagcgcc aggccgcggc gcgaggcctg
       61 caggcgggtg tggttctgga tgaaatcgtc ctcggacaag ccgaggtaag ccgcgatcgc
      121 cggaacttcc ggctcccgca gcttcacctc gccgggccag cggcagcagt tcccgcaacg
      181 ctggcattga taccacaccg cactcatggg taaaattctg cacgcgccgc acgccccgca
      241 caacttgaaa cgcttgaaac ttgccatcct cggaagcaac ggccgcctcg gcgccactct
      301 ctgccaggaa tggcgcgagc gcttcgaaat ccagccgatc ggccgcgcgc aactcgacct
      361 ggcccatgaa atggcggtgc gcgaatttgc ccgctcgatc caggcggacg cggtgctcaa
      421 ccccgccggg ctgacgagcc tcgaagcctg cgaagaccag cccggcgcgg cgaatgccat
      481 caatgcccgc gccccggagc tcctggccgc cggcttggcg gcgcgggaca ttccgctgct
      541 gcatttcagc acggactacg tcttcgatgg ctccaaatcc gggctttaca cggaagccga
      601 taccgcgcgg ccgatcagcc tttacgggag atccaagctc gcgggggagg aagccgtgct
      661 cgccgcctcg ccgcgccatt gggtgctgcg ggtttcgtgg ctgttcgatg gcggccgccg
      721 ggcgtttccc gatcagatcc tcgaacaggc ccggacccag gatcgcatcg aggccgtcgc
      781 ggacaaaatc tcggtgccga cctcctgccg cgacctcgcg gactggctgg cggacctgtt
      841 gccggacctt tccagggaag gcggccttta ccacgcctgc agccgcgggc gctgcacctg
      901 gcaggaatac gcgcaattca ttctcgacgc gatcccgcat ccgcgctgcc gccagctcac
      961 gccgatccgg ctgcgggacg ttccgttctt tcgcgccgcc cggccggcga attcggccat
     1021 ggacagcgcc cgtttctttc ggcggctcgg ccgggacgcg ccggactggc aaagcgcgtt
     1081 gacaaaattt ctccaccgcc tcacgaatcc tgcctgagtc cagccatgca ccgatttttt
     1141 gtttcacccg ccgatctcgc gaataacgcc ttcacgcttt cccccgaaga atcgcatcac
     1201 tgcctgagcg tcctgcgcca tcaaccgggc gaccgcgtca cgctcttcga cggccggggc
     1261 aacgaggcca cgggcatcct tggcggagtc gaaggcaagc tggcccaagt caaaatccag
     1321 ctgcgctcga aaaccgcgcc gctgcgctgc cggctgacat tggcccaggc aatcccgaag
     1381 gggaagaaca tggacctcgt cgtgcagaag gcggtcgagc tcggcgcggc ggccatcgcc
     1441 ccgattcttt cggagcgaac cgtcgtctcg ctggacggcg ccgagatcgc caaaaaacag
     1501 gaaaaatggc aggccctggc cctcgaagcc tgcaagcaat gcggccagaa ctttgtcccg
     1561 gaagtgctcg cgccgcagcc gctgaagcgt ttttttgaaa ccaaggccaa ggacgatctc
     1621 atgttgatcg cctcgctcca gcccgacgcc aagccggtca agacggtgct ggcggacgcc
     1681 ggcaaaagac cggccagcgt cacggtcttc gtcggacccg aaggcgactt cacccccgcg
     1741 gaactgggcc tggccaagag cagcggctgc cagcccgtca cgctgggacc gatcattctt
     1801 cggacggaaa ccgcggcgat ctattgcctc agcgtgctgg ggcacgagtt atattagccg
     1861 ccggtaaagg cctatcattt cctccaactg ccggctttcg gaaaaataat ggagggccaa
     1921 ttcgcgggag ttaccggccg cgccggcgga gcggttccac tcctccagcg cccggcgaac
     1981 gtccgcggcg cgaacggggt cgggcagcca gcgcacccca gagaaaccca gcgccttggc
     2041 cacccgcagg ccgggcgcct cggcgaggat cgccggcagg ccgcagccga aggcctgcag
     2101 catcgaaaaa gacaggccct cgtagcggct ggtgagaatg aagccatccg ccgcgcgcag
     2161 cagcgcttcg ggttggtcaa ggtaatggaa ggcgaacgcg gaactccggg cggcgggcga
     2221 aagcgactgc cgaagttcga ccgacccgtt tcccgcgtgc gcgaaggcgg cgccggagcc
     2281 gggcagaaag ccgtccagcg cggcgtagag cgggccatag tttttctgaa cggaagcgcg
     2341 cccgaccgtc acgagcaacc gggcctcggc cggcaacccc agctcgcggc gcaacctcgc
     2401 acgggtctcc gcatccgccg gggagaaccg ctccaggtcg atgccgtgat gaatgacgtg
     2461 gagcgagctc ggattgagct tcaggacccg gatggcgaaa tcgcgttcgt cctccgagca
     2521 gttgtgagtc ggcgccaagc ggcccagcag ggattcgatc ccgttgaaga aaaattcctt
     2581 tttgccgccg agccggggca ttccaaaata ggcgttcggg ctgtagagca ccggcgggaa
     2641 gccggggacc agcgcgccga gccgcgccag cgcgcccgcc ttggagctgt gggcgtgcac
     2701 gagctggatc cggcggcggc gcatccattg gagcagcgcc gccgcggcgg caaagtcgcg
     2761 gaattccggc gaattcccca cgcgcagatc gagggtttcc ccgccgcgcc gctcgaccgc
     2821 cgccacgagt ccgggcagcg ccccgcccgc ccggcggctg gaataagcca ggtccacccg
     2881 gatctccgga tgccggcggt gaaggtattc aatgaggctg cggacgcaag caaacacgcc
     2941 gtccaccccg ggttcgctca ccaccagcag ccgctccatc aaaacaactc cccgtagagt
     3001 ttcaaataac cccgagccgc ggccgcccag gaaaaccggg ccgcccaggc gcgtccttcg
     3061 gccgccaggg ccgccagcgc gggaggagcc agcgccagca attgccggag cccgccggcg
     3121 attgcctcca cggaataagg gtccacgagc gtggccggcc cggcgacttc ggggaggcag
     3181 gaaacattgg aggtcaatac cggcgcgccg agtgtcatcg cctccagcac cggcaggccg
     3241 aagccttcct ccagcgaggg ataacaaaac acccgcgcgc cggcataggc ctgggcgagc
     3301 ccggcctcgg agaggtatcc cagcaattgg actcccggcg cgctcagcag cgcgggcagc
     3361 tcgctttccg cggcaaaccg ggcctgcggc cggccgacga cgcgaagctc cagccccgga
     3421 aactccggtt ggattttcag ataagcccgg atgagccgga ccagattttt atgcggctgg
     3481 agcgagccga cgcagagcag ataatttccg cctcccgcgg gaccaggagc ggccagcggt
     3541 ttgacgccat tgtaaaccat gcgcaggcgc tcggggcgga ggttaaagct ctccgcggcc
     3601 tggccgcgga cgtagttgga aatggtcacc acgcgcgccg cgcgcttgag gccgggcacg
     3661 gcgccgaggg catacaccag gcggaaggag cgcgaatacc attccggatg ccggtggaaa
     3721 ttcaagtcgt ggagggtgac gacgttcttc acgccattct gccagaccgg gcaggtggtg
     3781 atgggatgat gcgccaggcg gcacccgtgt tgccgggcca gccggggcag gaaaaactgc
     3841 tcccagaact gcgcccaagc gcgcggccaa tcctgaaacg gcacttcgat ccatttgacg
     3901 ccgggaagcg tccgccactc ggaaataccg ggaaaccgtg agtcggcgaa aacgaccgct
     3961 tcaaaggagc ggggttcgcg caggatcgcg tcaaacagct ggaaagccac cgtctgggtg
     4021 cccgtcggtt gcggagttcc ggaaaaacgg ccgttaaacg cgactcggtg catgggcgga
     4081 tacggaggga tcggacgcat tgagccggcg cagtcttggc ggcagcccca ccgccagcca
     4141 ttgcagcacg agaaacgcgc ccatggacca aaaggtgaag gcatcgctgg tattggcgag
     4201 cggcaggcac atctgcatca tcaccacaac cttcaggccg caataaagac agggcgggcg
     4261 cttggcggaa atcaggtgca gggttcggag caatccgccg aacaggcatc ccatgatcac
     4321 cgccaggccc acgacgccgg ccgggccgaa gttgagaaac gactcgccaa aaaacgtgat
     4381 tcgcaatccg aaatgctcgt ccggattcca tcccaccatg cgaacgccgg taaggccgag
     4441 atgccattcc tttttttgcg gaaaaacgcc gctggggagc atggcggtca gtccgccgag
     4501 gtaggttttg ccattgagcg gctcgaaatc ccattcgctg aaaacccagg ccgcatcgcg
     4561 caggtcaatg agattgtccc ccagaaacgc ttcccgcagg ctctggccca cgatgttgcc
     4621 ttcgtccagc gcggagcgat cctggcgaag aatcgccgtg aagccgctga gcccggcgaa
     4681 caggaacagc gaagccgcaa acgcggcgac gagaaccggg cggggcagct ttttttccag
     4741 agtgacc
//
LOCUS       DATDAY010000225         2103 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_20429, whole genome shotgun sequence.
ACCESSION   DATDAY010000225 DATDAY010000000
VERSION     DATDAY010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2103
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20429"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..481)
                     /locus_tag="VIS74_04450"
     CDS             complement(<1..481)
                     /locus_tag="VIS74_04450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]
                     imidazole-4-carboxamide isomerase"
                     /protein_id="HEY5792524.1"
                     /translation="MLLLPAIDLMGGEVVRLQRGDPNLKTVYSNDPVAFAKKWEALGA
                     DWLHLVDLDAAFTGKCANLDAVRAICGAVSIPCELGGGMRSAEVLREAFDAGVRRVIM
                     GTKASQSLGFVTEMAAEFGSDRLAVGIDARNGRVAIKGWQEETSQSAAELAVAAQAAG
                     "
     gene            complement(518..1138)
                     /gene="hisH"
                     /locus_tag="VIS74_04455"
     CDS             complement(518..1138)
                     /gene="hisH"
                     /locus_tag="VIS74_04455"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HEY5792525.1"
                     /translation="MTRRIGIVDYQRGNLRSVQKALEKAGADARLISTPAEFGSMDGL
                     VLPGVGSFGDCAETLARTGLREPILEWLQSGKPYLGICLGYQLLFDSSEESPGTAGLG
                     FLQGTVGRFSNAVGKVPHMGWNALEFTEPRDPLFAGLSAVDQAYFVHSYYPRPADERI
                     VSTWCDYGGRFAAGIARGNVRGVQFHPEKSQAVGQKILRNFLALIQ"
     gene            complement(1221..1808)
                     /gene="hisB"
                     /locus_tag="VIS74_04460"
     CDS             complement(1221..1808)
                     /gene="hisB"
                     /locus_tag="VIS74_04460"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HEY5792526.1"
                     /translation="MSRTAEIERKTRETSIRLRLNVDGSGEASIRTGIGFFDHMLELF
                     AKHGLFDLEVEAQGDLQVDFHHTVEDTGIVLGQALAKALGDKAGIRRYGSAYVPMDEA
                     LVRVVLDLSGRPYLRYSAPGQVDAIGQFPFQLVEEFLRAVSVHGGMNLHADILEGRDA
                     HHMAEGLFKALAKALDFATQVDSRVKGVPSTKGSL"
     gene            complement(1805..>2103)
                     /locus_tag="VIS74_04465"
     CDS             complement(1805..>2103)
                     /locus_tag="VIS74_04465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792527.1"
                     /translation="AQDEGKRLGGIDLKPLRTAIGEAQLAARQIEQNQLALAETSLQN
                     AGAAWPQLELLPRLKKRLDDAKAAAAASNEAQAKAVQDAADLEKKESAAESKQP"
BASE COUNT          393 a          704 c          621 g          385 t
ORIGIN      
        1 cgcccgccgc ctgcgcggct accgccagct cggccgcgga ctgggaggtt tcttcctgcc
       61 atcccttgat ggccacccgg ccattccggg catcgatgcc caccgcgagc cgatcggacc
      121 cgaactccgc cgccatctcg gtcacgaatc cgagcgactg gctggccttg gttcccatga
      181 tgacgcgccg cacgccggca tcgaacgcct cgcgcaggac ttccgcggag cgcatgccgc
      241 cccccagctc gcagggaatg gaaaccgcgc cgcagatggc ccggaccgcg tccaggttcg
      301 cgcacttccc ggtgaaggcc gcgtccaggt ccaccaggtg cagccagtcg gcgccgagcg
      361 cttcccattt tttggcaaag gcgacgggat cgttggaata aacggtttta aggttgggat
      421 cgccgcgttg aagccgaacg acttcgccgc ccatgagatc aatggcaggt aataacagca
      481 taaagggaac cgccgaaacg cggagacacc gggaagttca ttggatcaac gccagaaaat
      541 tacggaggat tttttgcccc accgcctggc ttttttccgg gtgaaattgc acgccgcgga
      601 cattgccccg cgcgatgccg gcggcgaagc gcccgccgta atcgcaccaa gtgctgacga
      661 tccgttcgtc cgccggccgc ggataatacg agtgcacaaa ataagcctgg tccaccgccg
      721 agagtccggc aaagagcgga tcgcggggct cggtgaattc gagcgcgttc cagcccatgt
      781 gcggcacctt gccgacggca ttggaaaacc gcccgaccgt cccctgaaga aaccccaacc
      841 cggccgtgcc gggggattcc tcgctggagt caaacagcaa ttgatagccc aaacagatgc
      901 cgaggtaggg tttcccgctc tggagccact ctaaaatcgg ctcccgcagc ccggtccgcg
      961 ccagcgtttc cgcgcagtcc ccgaacgatc cgacgcccgg cagcaccagc ccatccatcg
     1021 agccaaactc cgccggagtc gaaatcaggc gcgcgtcggc gcccgccttc tccagcgcct
     1081 tctgaacgct gcggagattg ccccgctgat aatccactat gccgatgcgc cttgtcattt
     1141 tcgattctcg attttcgatt ctcgaatgag caagtccgat tgccgccaat cgaaaatccc
     1201 aaatcgaaaa tcgaaaatcc ctagaggctt cccttggtgc tgggcacgcc tttgacgcgg
     1261 gagtccacct gggtggcgaa atccagcgcc ttcgccagcg ctttgaagag gccttcggcc
     1321 atgtggtgcg catcccggcc ctcgagaatg tccgcgtgca ggttcatgcc gccatgcacg
     1381 ctgacggcgc gcaggaattc ctccacgagc tgaaagggaa attgcccaat ggcatctacc
     1441 tgccccggcg cggaataacg caggtaaggc cggccgctca agtccagcac cacgcgcacc
     1501 agtgcttcgt ccatgggcac ataggccgag ccgtaacggc ggatgcccgc cttgtcgccc
     1561 agcgccttgg ccagcgcctg gccgagcacg atgccggtat cctccacggt gtgatggaaa
     1621 tccacctgga gatcgccctg cgcctcgact tccaaatcga aaagcccatg cttggcaaag
     1681 agttccagca tgtgatcgaa gaacccgatg ccggtgcgaa tggacgcctc cccgctgccg
     1741 tccacgttca agcggaggcg gatggaggtt tcccgggttt tgcgttcgat ttcggcggtt
     1801 cggctcatgg ttgtttcgat tcggccgcgg attctttttt ctcgagatcg gcggcatcct
     1861 gcacggcctt tgcctgggct tcattgctgg cagcggcggc cgccttggcg tcgtccaggc
     1921 gtttcttcaa gcggggaaga agctccaact gaggccaggc agccccggca ttctgaaggg
     1981 aggtttcggc aagcgcaagc tggttctgct cgatctggcg ggccgccagc tgcgcttccc
     2041 caatggcggt ccggagcggc ttgaggtcaa ttccccccag ccgcttgccc tcgtcctgcg
     2101 cca
//
LOCUS       DATDAY010000226         3577 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_838314, whole genome shotgun sequence.
ACCESSION   DATDAY010000226 DATDAY010000000
VERSION     DATDAY010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 3577)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3577)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3577
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_838314"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..781)
                     /locus_tag="VIS74_04470"
     CDS             complement(<1..781)
                     /locus_tag="VIS74_04470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792528.1"
                     /translation="MTRILLALLLSLGCVQAQQVRRALPVESSPEERARFLAAIPLPP
                     DSRLAARQASPVYLSHIAPLNRMWERFNRANFQKQLQWSQAVMAPRIPPPPVLYYMFS
                     GPDFINAISLFPNCQTYIMCGLESVGQIAPPEALTDDQLAPSLGNLRKALGTILEFSF
                     FITKDMKADLEQTEFKGVLPILYTFLALSGCQIRDVQFLTLTPSGSLAAGGGGVPLVQ
                     IQFSRGASRPIQTLYYVRVNLADDGGNAGLFAWMQGKPAGAG"
     gene            complement(778..2076)
                     /locus_tag="VIS74_04475"
     CDS             complement(778..2076)
                     /locus_tag="VIS74_04475"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEY5792529.1"
                     /translation="MNRRINQVGLITCAALVIANMIGTGVFTSLGFQLAALSDPFAIL
                     GIWLLGGVFALCGAWSYAELASALPRSGGEYHFMGRVYHPALGFMAGIISIVAGFAAP
                     VALSAMAFGEYFRALCPEVPSTVSASALVALVTTAHCINLSSSRNFQVAFTVLKVTLV
                     GIFIGMGFGLPAHAAASLGPTAQTLPEVFKPEFAVSLMFAMYAYSGWNAATYIAGEVR
                     APQRTVAHSLVLGTLLVTVLYILLNAAFLYSTPAEALRGQLAIGRIAAEHLIGPAGGK
                     MVAAFICLGLISAVSAMTWTGPRVYQVIGEDYAALKFLARRSAGGIPRLALVLQSSLV
                     VIMIWSAAFESVLVFTQFALIACSLLTVVGVMVLRVRQPELERPFRVPLYPLPPVLFA
                     LIGIFTMGYSLKNNPVESMAGAAALGLALLLYPLARKKHP"
     gene            2240..2428
                     /locus_tag="VIS74_04480"
     CDS             2240..2428
                     /locus_tag="VIS74_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3008 family protein"
                     /protein_id="HEY5792530.1"
                     /translation="MPAKSKKQQMAAGAALSAKRGEIKKSSLKGASRQMVESMTEGEL
                     KELAETKRKNLPEKKSEE"
     gene            2826..>3577
                     /locus_tag="VIS74_04485"
     CDS             2826..>3577
                     /locus_tag="VIS74_04485"
                     /inference="COORDINATES: protein motif:HMM:NF024571.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY5792531.1"
                     /translation="MERLFRVHPAVAIEGPRQCGKTTLARMIGRREAVYFDLENPVDQ
                     RKLETPVQTLSPLRGLVVIDEAQRMPELFPPLRVLLDRVDADARFLLTGSASPELVRG
                     LSESLAGRVGVVDLAGFDLWETGTEEWRRLWLRGGFPRSYLAPDEVASADWRSGFVRT
                     FLERDLPQLGITVPAVTLRRFWTMVSHYHGQVWNASEFARSLGADEKTAGRYLDILAG
                     SFMVRVLPPWFENLKKRQLKAPKIYVRDTGLL"
BASE COUNT          756 a         1078 c         1145 g          598 t
ORIGIN      
        1 atcccgcgcc cgccggcttg ccttgcatcc acgcgaacag ccccgcgttc ccgccgtcgt
       61 ccgccagatt gacgcgaacg taatacagcg tctggatagg ccgggacgcg ccgcgggaga
      121 attggatctg cacgagcgga acgccgccgc cgccggccgc gagcgagccg gagggcgtga
      181 gcgtcaggaa ttgcacgtca cggatttggc agcccgacaa tgcgaggaac gtgtagagaa
      241 tgggcagcac gcccttgaat tccgtctgct ccaggtccgc cttcatgtcc ttcgtgatga
      301 aaaagctgaa ctccaggatc gtgcccagcg ccttgcggag attgccgagg gagggggcga
      361 gctggtcgtc cgtcaacgcc tccggcggcg cgatttgccc gaccgactcc agcccgcaca
      421 tgatgtaagt ctggcagttc gggaacaacg aaatcgcgtt gatgaaatcc ggcccgctga
      481 acatataata cagcaccggc ggcggtggaa tgcgcggagc catcaccgcc tgcgaccact
      541 gcagctgctt ttgaaaattg gcccggttaa accgctccca catccggttc agcggcgcga
      601 tgtggctgag ataaaccggc gacgcctgcc gcgcggccag ccgggagtcc ggcggcagcg
      661 ggatggccgc gaggaagcgc gcgcgttcct ccggggagga ttccaccggc agcgcgcgcc
      721 gcacctgctg cgcctgcacg cagcccaggg agagaagcaa tgccagaagg attcgggtca
      781 tgggtgtttt ttccgcgcca gagggtagag cagcagcgcc agtcccagcg ccgccgctcc
      841 ggccatggat tccacggggt tgttttttaa tgagtatccc atggtaaaga ttccgatgag
      901 cgcaaacaag accggaggca ggggataaag cggcacgcgg aagggccgtt ccagctccgg
      961 ctggcggacc cgcagcacca tcacgccaac caccgtgagc agggagcacg cgatgagcgc
     1021 gaactgcgta aagaccagca cgctctcaaa cgccgccgac cagatcatga tgaccacgag
     1081 cgagctctgc aaaacgagcg ccagccgcgg aatgcccccc gccgaccgcc gcgcgaggaa
     1141 tttcagcgcg gcgtaatcct cgccgatcac ctgatacacc cgcggccccg tccacgtcat
     1201 cgcgctgacc gcggaaatca ggccgaggca gatgaatgcc gccaccattt tcccgcccgc
     1261 cgggccgatc aggtgctccg ccgcgatgcg gccgatggcc agctggcccc gcagcgcctc
     1321 cgccggcgtg gagtaaagaa acgccgcatt gagcaggatg tatagcaccg tgacgagcag
     1381 cgtgcccagc accaatgaat gtgccacggt ccgctgcggc gcgcgaacct cgcccgcgat
     1441 gtaggtggcc gcattccagc cggaatacgc atacatcgcg aacatcagcg agaccgcgaa
     1501 ctccggcttg aatacttccg gcagcgtctg cgccgtcggc ccgaggctgg cggcggcgtg
     1561 cgccggcagg ccgaacccca ttccaatgaa aatgccgacc agcgtgactt tcagaaccgt
     1621 gaacgccacc tggaaattcc ggctggagga gagattgatg caatgcgccg tcgtgacgag
     1681 ggcgaccagc gcggaagccg acaccgtgga cggcacctcc gggcacagcg cgcggaaata
     1741 ttcgccaaag gccatcgccg aaagcgccac cggcgccgca aaaccggcga cgatggaaat
     1801 gatccccgcc atgaatccca gcgccggatg gtaaacgcgg cccatgaaat ggtattcccc
     1861 gcccgaccgc ggcagcgccg aggccagctc cgcgtagctc cacgccccgc acagcgcaaa
     1921 caccccgccc agcagccaga tgccgaggat cgcgaacgga tcactcagcg ccgcgagttg
     1981 aaacccgagg ctcgtgaaga cgcccgtgcc gatcatgttc gcaatcacca gcgccgcgca
     2041 cgtgatcagg ccgacttgat tgatacggcg gttcattcca cgccaagtgt gccagaaaac
     2101 cccggatgag cacgaatttt tgaccacgga tgaatgagtc ccgctcaaac cagtccgcgg
     2161 tagggcggtg tcgccgacac cgccgatttt ttcgcatttc cgaaacaacc gagacgttcc
     2221 tcactgtcga atcatccaca tgcccgcgaa atccaaaaag cagcaaatgg ccgccggagc
     2281 cgcgttgtcg gcgaagcggg gagagatcaa aaaatccagc ctgaagggag cctcccggca
     2341 aatggtggaa tccatgaccg aaggcgaact caaggaactg gccgaaacca aacgcaaaaa
     2401 cctgccggag aaaaaatcgg aggaataagc gggaacattc caccgcggat ggacgcggat
     2461 aaacgcggat ggaattttgc gggagggcgc aggtcttcgc tgccgaaacg tgatttttga
     2521 acagaaggcc gcaaagatcg ccaaggtttg gattaaatga actccctttg tgccctttgt
     2581 gagcttcggt tcaattatct gccggaaaaa atcggtaaat cggaggttga tcgaaacgcg
     2641 gaacaaactc ttgtcccgcc tcatctccgg caaaattctc cgccgagagc ttcgatagcc
     2701 aattcccacc cggcatggcg gaagaaagcg attttggaaa aatccatgaa aattaggagt
     2761 tgtcttctga tttttcatgg attattgctt tatgcatgat tgaaaggggg caagcgcgtg
     2821 cgcgggtgga gcgactcttc cgggtgcatc cggcggtggc catcgaaggg ccccggcagt
     2881 gtggcaagac caccctggcg cggatgatcg gccgccggga ggcggtttat ttcgacctgg
     2941 aaaacccggt ggatcagcga aagctggaaa caccggtgca gacactttcg ccgctgcggg
     3001 gattggtggt gatcgatgaa gcccaacgaa tgccggaact gtttccgccg ctgcgggtgc
     3061 tgctggaccg ggtggatgcg gacgcgcggt ttctcctgac gggcagcgcc tcgccggagc
     3121 tggttcgggg tttgtctgaa tccctggccg gacgggtggg tgtggtggat ttagcgggat
     3181 tcgatttgtg ggagacgggg acggaggaat ggcggcggct gtggctgcgt ggcggattcc
     3241 cccggtcgta tctcgcgccg gatgaggtgg cttcggcgga ctggcgctcg ggttttgtga
     3301 ggacatttct ggagcgggac ctgccgcaac tggggatcac cgtgcctgcg gtgacgctgc
     3361 ggcgtttttg gacgatggtt tcccattacc acgggcaggt gtggaatgcc tcggagtttg
     3421 cccgctcgct gggagcggac gagaagaccg cggggcgtta tctggacatt ctggccggct
     3481 ctttcatggt gcgggtgctg ccgccgtggt ttgagaacct gaagaagcgc cagttgaagg
     3541 ctccgaagat ctatgtgcgg gacaccggcc tgctgca
//
LOCUS       DATDAY010000227         3459 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_1200046, whole genome shotgun sequence.
ACCESSION   DATDAY010000227 DATDAY010000000
VERSION     DATDAY010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 3459)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3459)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3459
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1200046"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..392
                     /locus_tag="VIS74_04490"
     CDS             <1..392
                     /locus_tag="VIS74_04490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006983786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DnaB-like helicase C-terminal domain-containing
                     protein"
                     /protein_id="HEY5792532.1"
                     /translation="QLRSSSRRAQDNRQLEIAEISYGVKSLAKELDIPILVLAQLNRN
                     PDARGGKPRISDLRESGSIEQDADVVCMLTRPELYAKDEEEKNEDQGKAELIIAKQRN
                     GPIGEIALTFIKEFTRFEVQAFEREAT"
     gene            558..1484
                     /gene="epsC"
                     /locus_tag="VIS74_04495"
     CDS             558..1484
                     /gene="epsC"
                     /locus_tag="VIS74_04495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase EpsC"
                     /protein_id="HEY5792533.1"
                     /translation="MEPTLQRVAREILTSYRQVGGLNNSDGPNMPSKLAVGSICEELL
                     RLLFPGFHDEGAVHGHELTEITRSRISDIAERLDEEIFKSLRISDSACKHEQARTILY
                     RFLEALPGIRELLRADIEAAYEGDPAAMSYEEIVLSYPFIETVAVHRMAHVFYLAKLP
                     LLPRMMNEWAHSRSGIDIHPGATLGTHFFIDHGTGVVIGETCVIGNHVKLYHGVTLGA
                     RSFEKDEEGHLIKGGKRHPNVEDGVTIYPNSTILGGETVIGAGSTIGANVFLLQSVPA
                     QSLVVYEEKQLKILDKTRRNARAKSEVLEWSI"
     gene            1472..2626
                     /locus_tag="VIS74_04500"
     CDS             1472..2626
                     /locus_tag="VIS74_04500"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HEY5792534.1"
                     /translation="MVYLDYNATTPLAAEAREAMRPYLEDDFGNASSIHLAGRRARAA
                     VDDARERLAARLKARPHELIFTSGGTEANNLAILGIARKYRGRGRHLITAATEHHAVL
                     HPFEHLAKHEGFELTVLPVDERGVVDPEALRAAIRRDTILASVMSANNETGTRQDIAA
                     LAKICREHGVFFHSDVIQTFGKEPLYPHELGLDAISLAAHKFYGPKGAGLLWLRAGVS
                     LESIQYGGAHENQRRPGTENVAAIAGMAAAAELAGSGDLARIAGLRDALWEGIQTVCP
                     DAIRNGHPAQCLANTLNVSFPDADGETLLAGLDLEGICVSSGSACMVGSVQPSHVLLA
                     MGLPPGLAGATVRFSLGKFNQPPDIDKTLAALLRVWERQREFPIAHEVLT"
     gene            2610..3137
                     /locus_tag="VIS74_04505"
     CDS             2610..3137
                     /locus_tag="VIS74_04505"
                     /inference="COORDINATES: protein motif:HMM:NF021759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT-like domain-containing protein"
                     /protein_id="HEY5792535.1"
                     /translation="MKSSRDAILEHVVDDLLRYAGCPELAGRVQVEWNFRLRTTAGMA
                     HYQRRKITLNPRLVEVAADEVQRTLRHELAHIVAQYRAGRRRISAHGAEWKQACGDLG
                     IPHESRCHDLPFKIRRQARRHFYQCPECGFELARVRPAKRPIACLTCCRKHNGGHYDS
                     RFRFAPISEPRKLAA"
     gene            complement(3261..>3459)
                     /locus_tag="VIS74_04510"
     CDS             complement(3261..>3459)
                     /locus_tag="VIS74_04510"
                     /inference="COORDINATES: protein motif:HMM:NF013401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HEY5792536.1"
                     /translation="ISEAEEADTPLLGHLLATATRIAKAEGLRDSGFRLVINNGRDGG
                     ESVPHLHVHLLGKRPLAWPPG"
BASE COUNT          658 a         1120 c         1050 g          631 t
ORIGIN      
        1 agcagctgcg ctccagttcg cgccgcgcgc aggacaatcg ccagctcgaa atcgcggaaa
       61 tctcctatgg cgtcaagtcc ctggccaagg agttggatat ccccatcctg gtgctcgcgc
      121 agctcaaccg caatcccgac gcccgcggcg gcaagccgcg catttccgac ctgcgcgagt
      181 cgggctccat cgaacaggat gccgacgtgg tttgcatgct gacgcgcccc gagctttacg
      241 cgaaggacga ggaggaaaaa aacgaggatc agggcaaggc ggaactcatt atcgccaagc
      301 agcgaaatgg gcccatcggc gaaatcgcgc tcactttcat caaggaattc acccgtttcg
      361 aggtccaggc cttcgagcgc gaagccactt aaaaactaaa tcggattgcc aggattggct
      421 ggatttcagg attaattttc tgaggttaaa ggaattataa atatttccgg ttaatccggc
      481 aaatccggca aatcccgttt agtttgcggt ggcttggctc tggacatcgc tttccagccg
      541 cgcatagttg agggggtatg gaacccactt tgcagcgtgt tgcccgggaa atcctgacgt
      601 cctaccgtca ggtgggcggc ctgaacaatt ccgatggacc caacatgccg tccaaactgg
      661 cggttggatc catctgcgag gaattgttgc gtttgctgtt tccggggttc cacgatgagg
      721 gggccgtgca cgggcacgag ctcaccgaaa tcacccgttc gcgcatttcg gacatcgccg
      781 aacggctgga cgaggagatc ttcaaaagcc tgcgaatctc ggattccgcc tgcaagcatg
      841 agcaggcgcg cacgattctt taccggttcc tggaagcgct gccggggatt cgtgaactgc
      901 tgcgcgccga tatcgaagcc gcctacgaag gcgaccccgc ggcgatgagc tatgaggaga
      961 tcgtgctgtc ctatcccttc atcgaaaccg tcgccgtgca tcgcatggcg cacgtgtttt
     1021 acctggccaa gctgccgctg ctgccgcgca tgatgaatga atgggcgcat tcgcgttcgg
     1081 gcatcgatat tcatcccggc gcgacccttg gcacgcattt tttcatcgat cacggcacgg
     1141 gcgtggtcat tggcgagacc tgcgtcattg gcaaccacgt gaagctgtat cacggcgtga
     1201 cgctcggggc gcgcagcttt gagaaggatg aagagggcca cctcatcaag ggcggcaagc
     1261 ggcatcccaa cgtcgaggac ggcgtgacga tctacccaaa ctccacgatt ctcggcggcg
     1321 agacggtcat cggcgcgggt tcaaccatcg gcgcgaacgt tttcctgctc caaagcgtgc
     1381 ccgcgcaatc gctcgtcgtg tatgaggaaa agcaattgaa gatcctggac aaaacccggc
     1441 gcaatgcccg ggccaaaagc gaggtgctgg aatggtctat ctagactaca atgccaccac
     1501 gccgctggcg gcggaagccc gcgaggcgat gcggccgtat ctcgaggacg attttggcaa
     1561 tgcgtccagc attcatctgg ccggacggcg cgcccgggcg gcggtggacg acgcgcgcga
     1621 gcgtctcgcc gcgcggttga aggcccgtcc gcatgagctc atcttcacca gcggcggcac
     1681 ggaggcgaat aatctcgcca tcctcggcat cgcccgaaaa taccgcggcc ggggccgcca
     1741 cctcatcacc gccgcgaccg agcatcacgc cgtgctgcac cccttcgagc atctggcgaa
     1801 gcacgaaggc ttcgagttga cggtgctgcc ggtggacgag cggggagtcg tcgatccgga
     1861 agcgctgcgc gccgccatcc ggcgggatac gatcctggcc tccgtcatgt ccgccaacaa
     1921 tgaaaccggc acccggcagg acatcgccgc cctggcgaaa atctgccgcg agcacggcgt
     1981 gtttttccac agcgacgtca ttcagacctt cggcaaggag ccactttatc cccacgaact
     2041 cggcctggat gccatttccc tggccgcgca caaattttac ggcccgaagg gcgccggcct
     2101 gctctggctg cgggcgggcg tgtcccttga atccatccaa tacggcggcg cgcacgaaaa
     2161 ccagcgccgg cccgggacgg aaaacgtcgc cgccattgcc ggcatggccg ccgccgccga
     2221 actggccggc tccggggatc tagcgcgcat cgccgggctg cgcgacgcgc tctgggaggg
     2281 gattcaaacc gtgtgcccgg acgccattcg caacggccat cccgcgcaat gccttgccaa
     2341 tacgctcaac gtcagctttc ctgacgcgga tggcgaaacg ctcctggctg gattggattt
     2401 ggaaggcatt tgcgtctcca gcggctcggc ctgcatggtc ggttccgttc agccctcgca
     2461 cgtcctcctc gccatggggc ttccgcccgg gctggccgga gccaccgttc ggttctccct
     2521 tggaaaattt aaccaacccc cggacatcga taaaaccctc gctgccctct tacgtgtttg
     2581 ggagcggcag cgggaatttc cgatcgccca tgaagtcctc acgtgatgcc attcttgaac
     2641 atgtcgttga cgatctcctg cgctacgccg gctgtcccga gctggcgggg cgggtccagg
     2701 tcgaatggaa tttccgcctc cgcaccacgg cgggaatggc ccattatcaa cgccgcaaaa
     2761 tcaccctgaa tccccggctg gtggaagtcg ccgccgacga ggtgcagcgc acgctccgcc
     2821 atgagcttgc gcacatcgtc gcgcaatacc gcgccggacg ccgccgcatt tccgcccatg
     2881 gggcggagtg gaaacaagct tgcggggatc tcggcattcc ccatgaaagc cgctgccacg
     2941 atctgccctt caagattcgc cggcaggcgc ggcggcattt ttatcaatgt ccggaatgcg
     3001 gcttcgagct cgcccgcgtc cggccggcca agcgcccgat cgcctgcctg acctgctgcc
     3061 gcaaacataa tggcggccac tacgacagcc ggtttcgttt cgccccgatt tccgaaccgc
     3121 gaaaactggc ggcgtgatcc ggcggggaaa gccatggcgg tttcatcgaa cccgccccac
     3181 ctacaaaagg tagggacggc tcgccgagcc gtccgtcgcg gaaactgccg ccaccgaatt
     3241 gcctgcacga acgggctcgc tcaacccggc ggccaggcga gcgggcgttt gccgagcagg
     3301 tgcacgtgca ggtggggaac gctctccccg ccgtcgcggc cgttgttgat gaccaggcgg
     3361 aagccgctgt cccgcaagcc ttccgctttc gcgatccgcg tggcggtggc gagcaaatgg
     3421 cccagcagcg gcgtgtccgc ctcctccgcc tcgctgatc
//
LOCUS       DATDAY010000228         2516 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_1977708, whole genome shotgun sequence.
ACCESSION   DATDAY010000228 DATDAY010000000
VERSION     DATDAY010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2516)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2516)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2516
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1977708"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..356)
                     /locus_tag="VIS74_04515"
     CDS             complement(<1..356)
                     /locus_tag="VIS74_04515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792537.1"
                     /translation="MQLTEKLLLSMGGWQAFNEAKAMHRAGRVLEAAYEPPFLRGKIS
                     GERPLAAGLRIKNAVDVENLCPCRESRVRGVICAHSLAVGLQVISPLARPAATAAEVP
                     PGPSLAATEEAPVVEF"
     gene            complement(477..548)
                     /locus_tag="VIS74_04520"
     tRNA            complement(477..548)
                     /locus_tag="VIS74_04520"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(514..516),aa:Val,seq:tac)
     gene            660..1214
                     /locus_tag="VIS74_04525"
     CDS             660..1214
                     /locus_tag="VIS74_04525"
                     /inference="COORDINATES: protein motif:HMM:TIGR00645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HEY5792538.1"
                     /translation="MDQIAQPKSLATLGNFIFLSRWLQAPLYLGLIVAQAIYVYRFVI
                     ELWHLLHSAVAPAMDEKIIMISVLTLIDVVMISNLLIMVIVGGYETFVSRLRLDRHPD
                     QPEWLTHVNAGVLKVKLATALVGISSIHLLSTFIKIGIPTGPSSQDVLWQVIVHVTFV
                     VSALLLAITDRISTKTALEARPKH"
     gene            complement(1355..>2516)
                     /locus_tag="VIS74_04530"
     CDS             complement(1355..>2516)
                     /locus_tag="VIS74_04530"
                     /inference="COORDINATES: protein motif:HMM:NF015213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HEY5792539.1"
                     /translation="DVRGLLGESLAAAQAGNWRRAAEARLDAYVTFDLEIESRTLPRD
                     PSLALRAEKAFLDGDSRGPGIKAALDRRVKGEELAAAYQRALEGMDECAALLKVGLSP
                     GAAIASATVIIAREGLEAVVILAAILAGLRGAENAGVRKRLAGGAWLAVGVTILLFFV
                     SQSLLSGLSRYGETLEAVISILAVIIMLMVTNWVFHKMYWTEWNAKLRVMTKSAQRQQ
                     GTRMETWALIGVGFMTIFREGFETTLLMQSLILEAGLRSVSIGILFGCLLIAALGFVV
                     FYIGAKLPYRKMLVYTGGLVVFVLFTFVGSTVRLFQTVGWLPVHPIPGLEFPAWMGVW
                     LGLYPTWEGVLIPLLSFAYVGGAWLWVKWNSVRAAREAAEAVPETAPSSAVR"
BASE COUNT          495 a          836 c          751 g          434 t
ORIGIN      
        1 tggaattcca cgaccggcgc ctcctccgtg gcggccaatg acggcccggg cggaacttcg
       61 gccgccgttg ccgcgggccg ggcgagcggc gaaatcacct gcaaacccac cgccagggaa
      121 tgcgcgcaga tcaccccgcg aacccgcgac tcgcggcacg ggcagagatt ttccacatcc
      181 acggcgttct tgatccgcag cccggccgcc agcggccgct cgccggaaat tttgccccgc
      241 agaaacggcg gctcgtaggc ggcctccagc acccgcccgg cgcggtgcat cgccttggct
      301 tcgttaaagg cctgccagcc ccccatggag aggagcaatt tttccgtaag ctgcattgtg
      361 ggatcactct acgcccgcgc ggagattccc cgccagcgcg gctttattca atggcattgc
      421 ggtaataaca accacggacg gaaacagatt cacccggatg tatggataag aaaacctgcg
      481 ggctgagggg atcgaacccc cgaccaatag tgtgtaagac taccgctcta ccgctgagct
      541 aaacccgctg aaaatcggat gggcaccatg ccgcgcccgg ccgccaaacg caagcagata
      601 tcaccggcgg caatcggggg atgtcaaatt gccaggacgt gctaatatac ccgccgcata
      661 tggatcaaat cgctcaacca aagtcgctgg ccacgctggg aaatttcatt tttctgagcc
      721 gctggctgca ggcgccgctt tatctcgggc tgattgtcgc ccaggccatt tacgtttacc
      781 gtttcgtcat cgaattgtgg catctgctgc attccgccgt cgcgccggcc atggacgaaa
      841 agatcatcat gatcagcgtc ctgaccctga tcgacgtggt catgatttcg aatctcctca
      901 tcatggtcat cgtcggcgga tacgaaacct tcgtctcccg cctgcggctg gaccgccatc
      961 cggaccagcc ggaatggctg acgcacgtca acgccggcgt gcttaaagtg aagctggcga
     1021 cggccctcgt ggggatttcc tccattcacc tcctgagcac cttcatcaag atcggcatcc
     1081 ccaccggccc cagcagtcag gacgtgctct ggcaggtgat cgtccacgtg accttcgtgg
     1141 tttccgcgct gctgctggcc attaccgacc gcatttcgac caaaacggcg ctggaagccc
     1201 ggccgaagca ttagcctccg gcgaagccgt ggttttttta accacagaga acacaaagag
     1261 cacagagata aaaaccaggg gagcttcccc atttcccttc tttgcgctcg ctgcgttctt
     1321 tgcggttaat ttttgaacct ggcgcgcgcc gggatcagcg caccgccgac gagggggcgg
     1381 tttccggaac ggcttcggcg gcttcccttg ccgcgcggac ggaattccat ttgacccaca
     1441 gccaggcgcc gccgacgtag gcgaagctta acagcggaat cagcacgcct tcccacgtgg
     1501 gatacaggcc gagccagacg cccatccagg cgggaaactc gagtccggga atcgggtgca
     1561 ccggcagcca gccgacggtc tggaacaggc ggaccgtgga gccgacgaac gtgaaaagca
     1621 cgaagaccac gagcccgccg gtatagacga gcattttgcg atacggcagc tttgcgccga
     1681 tgtaaaagac cacgaagccc agcgcggcaa tcaacaggca gccgaacagg atgccgatcg
     1741 agaccgaccg gaggccggct tcgaggatga gcgattgcat gaggagcgtc gtctcgaaac
     1801 cctcgcggaa gatggtcatg aagcccacgc cgatgagcgc ccaggtttcc atgcgagttc
     1861 cctgctgccg ctgcgcgctt ttggtcatca cgcgcagctt cgcgttccat tcggtccaat
     1921 acatcttgtg aaacacccag ttcgtgacca tcaacataat gatcacggcg aggatggaga
     1981 tcaccgcttc gagcgtttcg ccgtagcggc tcaggcccga gagcagcgac tgcgagacga
     2041 aaaagagaag gatcgtcacg cccaccgcga gccaggcgcc gccggccagg cgcttgcgca
     2101 ccccggcatt ttccgccccg cgcaggccgg ccaggatcgc ggcgaggatc accaccgctt
     2161 ccagcccctc gcgggcgatg atgaccgtcg cgctggcgat ggccgctccc ggggaaaggc
     2221 cgaccttcag cagcgcggcg cattcgtcca tgccctccag cgcgcgctgg taggccgcgg
     2281 cgagttcctc gcccttcacg cgccggtcca gcgcggcctt gatgccgggc ccccggctgt
     2341 cgccgtcgag gaacgctttt tccgcgcgca gcgccagcga gggatcgcgc ggcagcgtgc
     2401 gggattcgat ttcgaggtcg aaggtcacgt aggcgtccag ccgcgcttcc gccgcccggc
     2461 gccagttgcc cgcctgcgcg gccgcgaggg attcgccaag caatccgcgg acatcc
//
LOCUS       DATDAY010000229         2882 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_194744, whole genome shotgun sequence.
ACCESSION   DATDAY010000229 DATDAY010000000
VERSION     DATDAY010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2882)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2882)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2882
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_194744"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..702)
                     /locus_tag="VIS74_04535"
     CDS             complement(<1..702)
                     /locus_tag="VIS74_04535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEY5792540.1"
                     /translation="MIPKPYYITTAIDYTNAAPHIGHAYEKVLADVLARYHRQAGREV
                     LFLTGVDQHGQKVQQAAQKAGVEPQAFADEVTKGFLSLWKLLGISYDRWAATTDASHK
                     AVVRKILQSLHDRGALYKAAHRGFYSVRQEQFLTDKERDESGNFGPEWGEVIEIEEEN
                     WYFRLSEHREWLAEFLARRPDFVQPNFRQAELANAVAKIGGDLCISRPKKRLAWGIEF
                     PFDPDFVTYVWFDALT"
     gene            complement(775..1098)
                     /locus_tag="VIS74_04540"
     CDS             complement(775..1098)
                     /locus_tag="VIS74_04540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY5792541.1"
                     /translation="MTLEELMDEASGDVALGELEAALAKYERCVELDPASFDAWHALG
                     MIRMKLGKIPEAIEAGLRAVAINPNDQLAWSSLSMFYVRNHQIPEAEAAGAKARIISW
                     GGKIQ"
     gene            complement(1095..2618)
                     /locus_tag="VIS74_04545"
     CDS             complement(1095..2618)
                     /locus_tag="VIS74_04545"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HEY5792542.1"
                     /translation="MINLLRQNQRWLMLIIVILTIIAFAWLYNPTDVNKLENSRIAEI
                     YGRTIHKVDIERQLKVYELAAILGETPFLEDLGSTAPTPEEAGSEFIWNLVVLQHEAR
                     QLGISPTDAQVVAAIQAVPGLQKNGTFDRDLYANVLQNVFAPRGMTERHLEDLVRDTL
                     KLARLRQIITSPVAVTDSEVAGAFRGFQAVDVQYVRLEPAADPAGAATPEEVEKVFNE
                     QKSAFATPEYRSIQYIRLSLPESSPALEGKARVDALQALAEQATQFVGRVLEKGEGFA
                     QAAAASNQSVIQTPFFDQTGAGAPQTPAPAANLPAPVVQASFSLTEAKPVSEILQDGD
                     AFYIAQLQKILPSRSLTLEEARPQIELRLVMQKTQETFQKFSEEQAQALKAAIQSGKT
                     FAQAAGEAGLTVQSLVNYPPFQPSPSQQPDENAVIARATLPLEAGEVSGFQPAPWGGF
                     LVQLTKRAPLDPMIYAQQHESVKTRILETKRNLLFIEWLTAAREEANLKFFAGQTSR"
     gene            2674..>2882
                     /locus_tag="VIS74_04550"
     CDS             2674..>2882
                     /locus_tag="VIS74_04550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792543.1"
                     /translation="MKASPTPGNYLIVLLFGTLIAGGQLVHAQDSVTLQNGQRRAGKI
                     TGVSGGNIQIQVPTPDGKGAVTTTLP"
BASE COUNT          510 a          895 c          897 g          580 t
ORIGIN      
        1 ggtgagcgcg tcgaaccaca cgtaggtcac gaagtcgggg tcgaagggga actcgatgcc
       61 ccaggcgagg cgcttcttcg gccgggaaat gcagagatcc ccgccgatct tcgcgaccgc
      121 gttggccagc tcggcctggc ggaaattcgg ctgcacgaag tccggacggc gggcgagaaa
      181 ctccgcgagc cattcgcgat gctcggagag gcggaaatac cagttctcct cctcgatctc
      241 gatgacctcg ccccattccg gcccgaagtt gccggactcg tcgcgttcct tgtccgtcag
      301 gaattgctcc tgccgcacgc tgtagaagcc gcggtgcgcg gccttgtaga gcgcgccccg
      361 atcgtggagg gattgcagga ttttccgcac caccgccttg tggctcgcgt ccgtggtggc
      421 cgcccagcgg tcgtaggaaa tcccgagcag tttccacagg ctgagaaatc ctttcgtgac
      481 ctcatccgcg aaggcctgcg gctcgacgcc ggctttctgc gcggcctgct ggaccttctg
      541 gccgtgctgg tccacgccgg tgaggaagag cacctcgcgg ccggcctggc ggtgatagcg
      601 cgcgagcacg tccgccagca ccttttcgta ggcgtgcccg atgtggggcg cggcgttcgt
      661 gtaatcaatc gcggtggtga tgtaataagg cttggggatc attttcgatt ggcgattctc
      721 gaatggcgcc ctcgttctca tcaatcgaaa atcgagaatc gccaatcgaa aatcctactg
      781 gatttttcct ccccaggaaa tgattcgcgc cttggcgcca gcggcttcgg cttccgggat
      841 ctggtggttg cggacgtaga acatcgagag gctggaccac gcgagctggt cgttgggatt
      901 gatcgccacg gcccggaggc ccgcctcgat ggcttccggg attttgccca gcttcatgcg
      961 gatcatgccg agcgcgtgcc aggcgtcgaa gctcgcggga tcgagctcca cgcagcgctc
     1021 gtatttggcc agggcggcct ccagctcgcc cagcgccaca tcgccgctgg cctcgtccat
     1081 gagctcctcc agcgtcatcg gctcgtttgg ccggcgaaga atttaagatt cgcctcctcg
     1141 cgggcggcgg tcagccattc gatgaagagc agattccgct tggtttccag aatccgggtt
     1201 ttcaccgact cgtgctgttg ggcataaatc atcggatcca gcggcgcccg cttcgtcagc
     1261 tggacgagga atccgcccca tggcgccggc tggaagccgc tgacttcgcc cgcttcgagc
     1321 ggaagcgtcg cccgggcgat taccgcattt tcatccggct gctggctggg cgacggctgg
     1381 aacggagggt aattcaccag cgactgcacg gtcagccccg cctcgcccgc ggcctgggcg
     1441 aaagtcttgc cagactggat cgcggccttc agcgcctggg cctgctcctc gctgaatttc
     1501 tggaaggttt cctgggtttt ttgcatcacc aggcggagtt cgatctgcgg gcgcgcttcc
     1561 tccagcgtga gggagcgcga tggcagaatt ttctgcaatt gcgcaatgta aaacgcgtcc
     1621 ccgtcctgga ggatctcgct caccggtttt gcttcggtca gcgaaaagct ggcttgaacc
     1681 accggagccg gcaggttggc cgcgggagcc ggagtctgcg gtgcgcccgc gccggtctga
     1741 tcaaaaaacg gcgtttgaat caccgattga ttcgaggcgg cggcggcctg cgcaaagccc
     1801 tcgcctttct ccagcacccg tccgacgaat tgggtggcct gctccgccag ggcctgaagc
     1861 gcgtccaccc gggccttgcc ttccagcgcc ggcgacgact cgggcaggct gaggcgaatg
     1921 tattggatgc tgcgatactc cggcgtcgcg aaggcgcttt tctgctcatt aaaaaccttc
     1981 tccacttcct ccggggtggc ggcgccggcc ggatcggcgg cgggttccag ccgcacatac
     2041 tggacatcca cggcctggaa gccgcggaaa gcgcccgcca cttcggaatc ggtcaccgcc
     2101 accggcgagg tgatgatttg ccgcaggcgg gccagcttca gcgtgtcgcg cacgagatcc
     2161 tccagatgcc gttcggtcat gccgcgcggc gcgaagacgt tttgcagaac atttgcgtag
     2221 agatcgcggt cgaacgttcc atttttttgc agccccggaa cggcttgaat ggcggcgacc
     2281 acctgcgcgt ccgtcgggga gatccccaat tgccgggcct cgtgctgcag caccaccaaa
     2341 ttccagatga actcggaacc ggcctcctcc ggcgtcggcg ccgtgctgcc cagatcctcc
     2401 aggaacggcg tctcgccgag gatggccgcc agctcataga ccttcaactg gcgttcgata
     2461 tccaccttgt gaatggttcg cccgtaaatt tcggcgatgc gggaattctc gagcttgttg
     2521 acatcggtcg ggttatagag ccaggcgaaa gcgatgattg tcagaatgac aatgatcaac
     2581 atgagccagc gttgattttg acggaggaga ttaatcatgc gatgatgaga aaaagttgat
     2641 ttgggaagcg aatgtgatta gtccatgcct ttcatgaaag caagcccgac ccccgggaac
     2701 tatttgatcg tcctgctttt cggcaccttg attgccggag gccaactcgt tcacgcccag
     2761 gattcggtga ccctgcaaaa cggccagcgc cgcgcgggga aaatcaccgg cgtttctggc
     2821 ggaaacattc aaatccaagt tcccacgccg gacggcaaag gcgccgtcac caccaccctg
     2881 cc
//
LOCUS       DATDAY010000230         6715 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_1478350, whole genome shotgun sequence.
ACCESSION   DATDAY010000230 DATDAY010000000
VERSION     DATDAY010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 6715)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6715)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6715
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1478350"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(112..303)
                     /locus_tag="VIS74_04555"
     CDS             complement(112..303)
                     /locus_tag="VIS74_04555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792544.1"
                     /translation="MTTRFTYWKETDGKYLGFLNDYPDHWIQGEDMEDLKAHLRDLFA
                     TFSAEELPGIRKEEELEIA"
     gene            complement(460..807)
                     /locus_tag="VIS74_04560"
     CDS             complement(460..807)
                     /locus_tag="VIS74_04560"
                     /inference="COORDINATES: protein motif:HMM:NF024788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLDc N-terminal domain-containing protein"
                     /protein_id="HEY5792545.1"
                     /translation="MNRLPLLVISLLFFVMSGALVYLFFSFNSASPEQQQSYFSSLLR
                     FFPLGIVIAAFAGFILWISALIHMLMNRSLVGNDKIVWVLVIIFLNALGALLYFILLP
                     FIQNLTRAKPINE"
     gene            complement(1120..1566)
                     /locus_tag="VIS74_04565"
     CDS             complement(1120..1566)
                     /locus_tag="VIS74_04565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792546.1"
                     /translation="MKPLLLILSVICLIIDTQARIVPAWPFSEMEKRADLVIIGIPKP
                     TEETGEKIQIEKTPFNSIHPPFNAIGLNTPFQVEAVLKGDKKIKEIILHHYRTDASLP
                     DGPSLVRFDKTNRSPYLLFLSREPDGRYQAVTGQIDPAFSIRKLEN"
     gene            complement(1689..2069)
                     /locus_tag="VIS74_04570"
     CDS             complement(1689..2069)
                     /locus_tag="VIS74_04570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY5792547.1"
                     /translation="MITRLDHLVLTVSDIPRSVNFYTKVLGMREVVFGANRAALSFGN
                     QKINLHERGKEFDPKAASPTPGSADLCFISDSPIDLILSDLEAHGVPIIEGPVSRTGA
                     RGLILSVYFRDPDGNLIEVSNDVE"
     gene            complement(2066..2563)
                     /locus_tag="VIS74_04575"
     CDS             complement(2066..2563)
                     /locus_tag="VIS74_04575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792548.1"
                     /translation="MWGDRIDLSSTSTFAKNMSAWRRKAIELLPEYKALIEGCETPMF
                     LWTELHLAFHRVAEEENRPLMQRLFDYARWCWLESPSQDTQTAALLGFYENIPSDRRH
                     WWTLATSVARPDFKALEQVFRYFLSEEQFAEFTADYLRNRDLIEKQILKNKKRTRPRT
                     SGDNS"
     gene            complement(2722..2919)
                     /locus_tag="VIS74_04580"
     CDS             complement(2722..2919)
                     /locus_tag="VIS74_04580"
                     /inference="COORDINATES: protein motif:HMM:NF021253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module protein"
                     /protein_id="HEY5792549.1"
                     /translation="MIARQEIRQMPFEEKLALLEAVWTEISAEPEKIEVPRWHKEILD
                     ERDQALRSGKESIWTGSEAET"
     gene            complement(2916..3185)
                     /locus_tag="VIS74_04585"
     CDS             complement(2916..3185)
                     /locus_tag="VIS74_04585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792550.1"
                     /translation="MKSESNGMHSLFQGFTIIRHPLISTPPRSPAFQQVGEFQFRSGR
                     PTFRIREITAADHRLNSKPWPIISIQILFGKTALLHFSNNGILQS"
     gene            complement(3973..4488)
                     /locus_tag="VIS74_04590"
     CDS             complement(3973..4488)
                     /locus_tag="VIS74_04590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792551.1"
                     /translation="MRKGESSNRFGFRILLLALAGLIAGCAGPSAFRGFQSIEQRRGI
                     LRWQQPNGNGLTAESYFSRGGDGAISLVIGKEGPTPLLEGILQNGTASFRGPLLGGLA
                     WAGPVNAAPKSLAPWAALLSAYAQGATLADGSQEVHTGAYRAQFQKSAGRLTAFYVVC
                     NDTGDSFTVRF"
     gene            complement(4499..5509)
                     /gene="gnd"
                     /locus_tag="VIS74_04595"
     CDS             complement(4499..5509)
                     /gene="gnd"
                     /locus_tag="VIS74_04595"
                     /inference="COORDINATES: protein motif:HMM:TIGR00872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HEY5792552.1"
                     /translation="MQLGMIGLGRMGANMVRRLMKQGHQCVVFDTNPDSVQALVADGA
                     IGASSLEEFAAKLEKPRNAWIMIPAAITDKIVNELAGVLAAGDAIIDGGNSYFRDDRR
                     RAAALAPKGIHYVDCGTSGGVFGLERGYCLMIGGDKEAVDRLDPIFKTLAPGIGSAER
                     TPGREKLGGTAEEGYLHCGPPGAGHFVKMVHNGIEYGLMAAYGEGLDILANANIANQP
                     QEKDAETAPLMNHDLVYDLNLADITEVWRRGSVVSSWLLDLAAAAFADDPHLEKFSGR
                     VSDSGEGRWTIMAAIDEAVSCETLTAALYSRFRSRRDHTVAEKVASAMRFQFGGHLEK
                     KA"
     gene            complement(5559..>6715)
                     /locus_tag="VIS74_04600"
     CDS             complement(5559..>6715)
                     /locus_tag="VIS74_04600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792553.1"
                     /translation="HDSKVETFLEGEGLRPFRVSPNSVDLQHPRRAANESAATVTLAN
                     PQSRNLILSVEAPPFVEAPDSVEIPAAGTAELTLRVAPEKLTGGEGKIVLSEQSDRAE
                     IAVRVFPAPALLQTEPAETFALGRVEKNDSLRARFRILNQGGSTTEIQIETPSAVSLT
                     SPRTLTLAPGQQQEITFAFQPATLGAYEDALHLLYDARKQSLALTAQVEAPGSLPASR
                     PGEVSVPAPAAAPIGLSFQKLFVLEQGHDGVKLAWLKPSGEPPAAYRVDRRQLRRGER
                     DEVQEVWEPLPNVSYGEEGNAHTAQISGLQAGQRLQIRIFSLEANGNITATSTAFSIA
                     TKAYAPRRPLPWLWAIPIGAVLAGLAWRIRGRRREARKRDLARLDEFTKA"
BASE COUNT         1403 a         1817 c         2049 g         1446 t
ORIGIN      
        1 ttgggtattt cggaaatctc ttttgagaaa tactatagat gtcatgtcgt ccgccatggc
       61 gtagaatctg acgcccggag tcttcaagct tcgagatgag gtcgcgcctc ttcaagcgat
      121 ctcaagttct tcttcttttc ggattccagg gagttcctcg gcggagaatg tcgcaaaaag
      181 atcacgaagg tgggctttga gatcctccat gtcctcccct tggatccaat gatctggata
      241 atcgttgagg aacccaagat atttgccatc agtttccttc caataggtaa accgagttgt
      301 catggagtca atatggcatc tgccggcggc gcaggcgagg aggattcctt attcatctca
      361 cattcagccg ccccgatcaa gcgatcaaac tttaagctgc ggataaaaat aaggcaggca
      421 accgtaaaaa gttgccgggt tattattaga ggtggcatct tattcgttga ttggttttgc
      481 ccgagtgagg ttttggatga atggcagcaa gatgaaatag agcaatgcgc caagggcatt
      541 cagaaatatg atcaccagaa cccagacgat tttatcgttc ccgaccaggg agcgattcat
      601 gagcatatgg atcaaggctg aaatccagag aataaacccg gcgaatgccg cgatcacaat
      661 cccaagggga aaaaaccgga ggagggatga aaaatagctc tgctgctgct ccggcgaagc
      721 cgagttaaag ctgaaaaata gataaacgag cgccccgctc atgacgaaaa acaggagaga
      781 gataaccaaa agtggaaggc ggttcattgg gattggaatt tgtccgattg gatcatgggt
      841 tgattgcgcg ggtctttcaa aagttgggta gcactttctt ttatctcacg caaaggcgca
      901 aagccgcgaa gggaagagaa tccggtcgat cagggcatgg cgttatcgtc gattggcccc
      961 ccgcccagtt gaacggattt aatgatccca ccgtggaaaa tcgaatcgtt tgggtggagg
     1021 aatgccggtt gccggcgctc cggcgcttgc gttcctcgaa gatcagggcg gcaagaatct
     1081 gccgccctcc agaagcgagg tgtccgtttg cggcgccttt tagttttcca gtttgcggat
     1141 cgagaaggct ggatcaatct gtccggtaac cgcctggtaa cggccatcgg gctctcgcga
     1201 gagaaagagg agatacggag atcgattcgt tttatcgaaa cggactaaag aagggccgtc
     1261 gggcagggag gcatccgtgc gatagtggtg aagaataatc tctttgatct ttttatcccc
     1321 tttcaacacc gcctcgactt gaaatggggt attgagtcca atggcgttga agggcgggtg
     1381 gatgctattg aacggcgtct tctcaatttg gattttctct ccggtttctt cggtcggctt
     1441 cggaattccg ataatcacaa ggtcggcccg cttctccatt tcggagaaag gccacgccgg
     1501 aacgattcga gcttgcgtgt caataatgag acagatgacg gataaaatca ggaggagggg
     1561 tttcatgttt cagggcggac atctcagcta agcggccaat gcgcccgcct gatcatggca
     1621 ggaagttagg gacattccta gccgaatcaa tttggcccta agcctaggta gggcgccttg
     1681 atcggcgctt attcgacatc gtttgagact tcaatgagat tgccatccgg gtcgcggaaa
     1741 taaaccgaaa ggatcaggcc gcgggctccg gttctggaaa ccgggccctc aataatcgga
     1801 acgccgtgag cttccaaatc cgaaaggatg aggtcgatgg gcgaatcgga aataaaacag
     1861 agatcggcgg agcccggagt gggggaagcg gccttgggat caaactcttt gccccgttca
     1921 tgaagattga tcttctgatt tccgaatgaa agagcggcgc gattggcgcc aaaaaccact
     1981 tccctcatgc ccagaacctt cgtgtaaaag ttcacggaac ggggaatatc gctaaccgtg
     2041 agaaccagat gatcgaggcg ggtgatcatg aattatcccc ggacgtccgc ggccttgttc
     2101 gctttttatt cttgagaatc tgtttttcaa ttaaatcccg atttcgaagg taatcggccg
     2161 tgaactcggc aaattgttcc tcactgagga agtagcgaaa cacctgctca agcgccttaa
     2221 aatccggccg ggccacagaa gtggcgaggg tccaccaatg tcggcggtcg gagggaatat
     2281 tctcgtagaa accgagcaat gccgccgtct gggtatcctg cgagggtgat tccagccagc
     2341 accaccttgc gtaatcgaac aggcgttgca tcaacggcct gttttcctcc tccgccaccc
     2401 gatggaacgc gagatgcagt tcagtccaaa ggaacatggg cgtctcgcag ccttcgatca
     2461 gagccttgta ttccggaagc aattcaatgg ctttgcggcg ccaggcgctc atgtttttag
     2521 cgaacgtaga agttgaactg aggtcgatgc ggtcgcccca caagaattat cgagtgaaaa
     2581 aggcgggaag ttcggaacgg agtccacggg acgaatgtag cccttggctc aagcgtcttg
     2641 tgcgatgttt attgaatccg tcgattaggt catcgcgcac tgaggcgaga atttggactt
     2701 tcacctaatg gctcggccga tctatgtttc agcttcactc ccagtccaaa tggattcttt
     2761 tccagaccgc aaagcttggt cacgttcatc gaggatctcc ttatgccacc gtggcacctc
     2821 gattttttcc ggctcagcag atatttcggt ccaaacagcc tcaagcagcg caagtttttc
     2881 ctcaaaaggc atctggcgaa tttcttgccg tgcaatcatg attgaaggat gccgttattt
     2941 gagaaatgaa gcaatgccgt tttaccgaac agtatttgaa ttgaaataat tggccagggt
     3001 ttcgaattaa ggcgatgatc ggcggcggtg atttctcgaa ttctaaaagt tggcctgcct
     3061 gaacggaatt ggaattcgcc gacttgttgg aaggccggtg agcggggggg agtcgaaata
     3121 agcggatggc ggataattgt aaatccctga aaaagtgaat gcatcccatt ggattctgat
     3181 ttcattacat ggattatcca gtggatcatt ccgaggcggg atgacgtttc ccacatccca
     3241 aacggcaaag tcgattttct gaatggcgag atctacttcc cgaatggtgg attccctttt
     3301 ccgaatgacc aagttgagtt cccgaatggc ggtctgaact tcccatataa ggaaggataa
     3361 ctcccgaatg acgcattcca cttcccgaat gacgaagcgc atctcccgaa tgctcaactt
     3421 gatttcccga atggagcctt ttagagcgat tctcaaaaga gatttccgaa atagccaacg
     3481 ggttgttgag gcgcttgtcg ggtggggaaa tgggggcccc gtcgccccat tggacgcctt
     3541 tgtttgcggg aaaacgggga ccccgcatcc ccgtttacgc tgggcggttg gcacaattgc
     3601 cgcacttcgt gcgcccctcg ggctgtcccg catccgcggg acaggctgtc ttcgctccgg
     3661 ctgcccctac aatgaatcgc ttttcggaaa tctcttttga gaaatactat agctccattg
     3721 cggcgggctg aatctccaga agccgaagtt ctgttttttt gcctcacgcc ccgcagtcgc
     3781 gggacgcaaa gccgtgaacg aggagggttc cgccgaccgg gatagagttt ttgttcaacc
     3841 ggacgcaaga agcgaggcga cggacggctc ggcgagcccc tacccaggtg gcagggcggt
     3901 gtcgccgaca ccgccgtggc ttcctccgcg tgagcccttg cctgcatcgg tgaaaaacgg
     3961 cgcgaatccg ctctaaaacc gaaccgtgaa cgaatccccc gtatcgttgc acaccacgta
     4021 aaatgcggtg agccgcccgg cggatttttg gaactgggca cggtaggccc cggtgtgaac
     4081 ttcctgagag ccgtccgcca gggttgcgcc ctgcgcgtag gcgctaagca gcgcggccca
     4141 gggcgccagc gatttgggcg cggcattgac cggcccggcc caggcaagcc cgccgagcag
     4201 cgggccgcgg aaggaagccg tgccattttg caggatgcct tccagcaagg gggtcggccc
     4261 ctccttgccg atcacgaggg agatcgcgcc atcgccgccg cgggagaaat agctttccgc
     4321 cgtcaggccg ttgccattgg gctgctgcca gcgcaggatc ccgcggcgtt gttcgatgga
     4381 ttggaatccc cggaaggcgg aaggccccgc gcagccggcg atcaatccgg ccagcgcgag
     4441 gagaaggatt cgaaacccga accggttgct ggattccccc ttccgcattt ttcggaaatt
     4501 acgctttttt ctccaggtgc ccgccgaatt ggaaacgcat ggccgaggcg accttttcgg
     4561 cgacggtgtg atcgcgccgc gaacggaagc gcgaatacag cgcggcggtc agcgtctcgc
     4621 acgacaccgc ctcgtcaatg gcggccatga tggtccaacg gccctcgccg gaatcggaca
     4681 cgcggccgga gaatttttcg agatgcgggt cgtccgcgaa ggccgccgcc gccaggtcca
     4741 gcagccagga ggaaaccacg ctgccgcgcc gccagacttc ggtgatgtcg gcgaggttga
     4801 ggtcatagac cagatcgtga ttcatgagcg gcgcggtttc cgcgtccttt tcctgcggct
     4861 ggttcgcgat gttggcgttc gcgaggatgt cgagcccttc gccgtaggcc gccatgaggc
     4921 cgtattcgat gccgttgtgc accatcttga cgaagtgccc cgcgcccggc gggccgcagt
     4981 gcaggtaacc ttcctccgcc gtgccgccga gtttttcgcg gccgggagtg cgttcggccg
     5041 agccgatgcc gggagcgagg gttttgaaaa tggggtcgag gcggtccacg gcttccttgt
     5101 ctccgccgat catgaggcaa tagccgcgct ccagtccgaa gactccgccg ctggtgccgc
     5161 agtccacgta gtgaattccc ttcggggcga gggcggcggc gcggcggcgg tcgtcgcgga
     5221 aataggaatt gccgccgtcg atgatcgcat cccccgccgc gagcaccccg gcgagttcgt
     5281 tcacgatttt gtcggtgatc gcggccggga tcatgatcca ggcattgcgc ggtttttcga
     5341 gcttcgcggc aaattcctcg agcgagctgg cgccgatcgc gccatcggcc acgagcgcct
     5401 gcacgctgtc cgggttggtg tcgaagacga cgcattgatg cccttgtttc atgagacggc
     5461 gaaccatgtt cgcgcccatc cggccgagac caatcattcc gagttgcatg gcggctccac
     5521 tctcgcctca gcctcggatg gcggcaaatc ttttcgcgtt acgccttggt gaattcgtcc
     5581 agccgcgcca gatccctttt gcgggcttcc cgccgccggc cgcgaatccg ccaggccagc
     5641 ccggcgagaa ccgcgccgat gggaatggcc caaagccacg ggaggggccg gcggggggcg
     5701 taggccttgg tggcgatgga gaaagcggtg gaggtggcgg tgatatttcc gttcgcctcc
     5761 aggctgaaaa tgcggatttg cagccgctgc ccggcttgca ggccggagat ttgcgccgtg
     5821 tgggcattgc cctcctcccc gtagctgaca ttgggcaatg gctcccagac ttcctgcacc
     5881 tcgtcgcgtt cgccccgccg caattgccgg cggtccacgc gatacgccgc gggcggttcg
     5941 ccggagggtt tgagccaggc gagcttcacc ccgtcatgcc cttgctcgag gacaaacaat
     6001 ttctgaaacg aaaggccgat cggcgccgcc gccggcgccg gcaccgagac ctctcccggg
     6061 cggctggcgg gcaggcttcc cggagcttcc acctgggcgg tcagcgccag ggattgcttg
     6121 cgcgcgtcgt aaagcaggtg gagggcgtct tcataagctc ccagggtcgc gggctggaag
     6181 gcaaaggtga tttcctgctg ctggcccggc gcgagcgtga gggttcgcgg gctcgtcagg
     6241 gaaacggccg agggggtctc gatctgaatt tcagtcgtgg agcctccctg gttgagaatg
     6301 cggaagcggg cgcggagcga gtcatttttt tccacccggc ccagcgcgaa ggtttcggcg
     6361 ggttcggttt ggagaagggc gggcgcggga aagacgcgca cggcgatttc ggcccggtcg
     6421 gattgttcgg acaggacgat tttgccttcg ccgccggtca gtttttcggg ggccacccgc
     6481 agggtgagtt cggcggtgcc ggcggcgggg atttcgacag aatccggagc ttccacaaag
     6541 ggcggggctt cgaccgagag gatgagattc cgggattgag gattggccag cgtgaccgtg
     6601 gccgcgcttt cgttggcggc tctccgcgga tgctggagat cgaccgaatt gggcgaaacg
     6661 cggaagggcc gaaggccttc cccctccagg aaagtttcca cttttgaatc gtggg
//
LOCUS       DATDAY010000231         2482 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_2336103, whole genome shotgun sequence.
ACCESSION   DATDAY010000231 DATDAY010000000
VERSION     DATDAY010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2482)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2482)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2482
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2336103"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2482
                     /locus_tag="VIS74_04605"
     CDS             <1..>2482
                     /locus_tag="VIS74_04605"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEY5792554.1"
                     /translation="PWLLAGAAAVILAASFFAITRENRFDSEILNLLPADNRAVQGLK
                     IYNRDFTQTRELAFLIRGESAEAGENFADFLIPKLRQQPWTLRLLEGAPLETPEGRET
                     FPSLGAALLLNLPPDQFPAALAGLNAADLPARLKRLVQQVRAGSPRAEFELENDPLGL
                     LAPALAPLAKTISLETDFALVSPNGATRLIPVIVRPELPDDTACAALMDQVHAFIAQV
                     RSEYGPAAPIVEVTGRAAYVAEISTSMKRDLRLTSLISLAAISGLFWLGYRRVFPLAG
                     AALILSGCALAAMGTGTWFYPSLNVVAIGFCSILFGLGNDYSLLLYEAWREARRRAPD
                     FAGAVRGTLDHVLPGVVFVAFTTCIGFLALRFSGSIGFAQLGVLTSLGVAFCAAAMLT
                     LFPLWLRFHRPAEAAPTEAFYHSLQNTLADLPPVMGRGLLAGFIGVTLLALLPWRPLA
                     FDLSPASLEPRNIPASRALAHLQQAFPDSSEPVMIAGRGAALAERVDARLIELKRAGE
                     IAGFSGASALLVHPDRAARNRALWQSHPAGESELAEWFSQAGLDYSQFPETHRLLEAL
                     RQEGPPLPESSPWWFLRDRLISGDQFIAYVRPAKNRPFTEALFGPLARETLVTGWSTL
                     LGSLVPWAQRELIVYGGAVAGIILLVLAAIYRDARVWGVHVLGLIFAYGGLAATLKLL
                     GVKLNLLNVLAFPLILGTGVDYGTHLLLTLRHGEKWREHLTLVFRPILFGALTTVAGF
                     AALIPASSPALSGLGLVCALGVAWTLISALFFILPAMAWMGRANRPRPARPPSPPSLL
                     LIAVAILSIVLPSPSANADDALRLAQIEL"
BASE COUNT          318 a          891 c          776 g          497 t
ORIGIN      
        1 agccgtggct gctcgccggg gcggcggcgg tcattctcgc cgcgagtttt tttgccatca
       61 cgcgcgagaa ccggttcgat tcggagattc tcaacctgct gcccgcggac aatcgcgcgg
      121 tgcagggact taaaatttac aatcgggatt tcacccagac ccgcgagctc gcgtttctca
      181 tccgcggcga atcggcggaa gccggggaga acttcgcgga ttttttgatt ccgaaattgc
      241 gccagcagcc ctggacgctg cggctgctcg aaggcgcgcc cctcgaaacg cccgaggggc
      301 gcgaaacctt tccctccctc ggcgccgcgc tgctgctgaa tctgccgccg gatcaattcc
      361 cggccgccct cgccgggctc aacgccgccg atctgcccgc ccggctgaag cggctcgtcc
      421 agcaggtgcg ggccggttcg ccgcgcgcgg aattcgagct tgagaatgat ccgctggggt
      481 tgctcgctcc cgcgctggcg ccgctggcga aaacgatctc gctggaaacc gatttcgccc
      541 tggtctcgcc caacggcgcc acgcggctga ttcccgtgat cgtccgtccg gagctgcccg
      601 acgacaccgc ctgcgcggca ttgatggatc aagtgcatgc gttcattgcc caggtccggt
      661 ccgaatacgg ccccgccgcg ccgatcgtgg aagtcaccgg ccgcgcggcc tacgtggcgg
      721 aaatttccac aagcatgaag cgggacctgc ggctcacgtc gctcatctcc ctcgcggcca
      781 tctccgggct tttctggctc ggttaccgcc gcgtgtttcc gctcgcgggc gcggcgttga
      841 ttttaagcgg ctgcgcgctg gcggccatgg gaacgggcac gtggttttat ccctcgctca
      901 atgtcgtggc catcgggttc tgctcgattc tgttcggcct gggcaatgac tacagcctgc
      961 tcctttacga ggcctggcga gaggcccggc ggcgcgcgcc ggattttgcc ggggcggtgc
     1021 gcggcacgct cgatcacgtc ctcccgggag tcgtgtttgt cgccttcacc acctgcatcg
     1081 gcttcctcgc gctgcgtttc agcggctcca tcggctttgc ccagctcggc gtgctgacct
     1141 cgctcggggt cgccttctgc gcggcggcca tgctgacgct ctttccgctc tggctgcgct
     1201 tccaccgccc cgccgaggcc gcgccgacgg aagccttcta tcactccctg caaaatacgc
     1261 tcgccgatct gcctccggtc atgggccgcg gattgctggc cggattcatc ggggtcacgc
     1321 tgctggcatt gctgccgtgg cgccccctcg cctttgatct ttcccccgcc tcgctcgaac
     1381 cgcggaacat tcccgccagc cgggcgcttg cgcacctgca gcaggcgttc ccggattcgt
     1441 ccgagccggt gatgattgcc gggcgcggcg cggcgctcgc ggagcgcgtg gacgcgcgcc
     1501 tgatcgaact caagcgcgcg ggagaaatcg ccggtttctc cggcgcgtcc gccctgctgg
     1561 ttcatccgga ccgcgccgcc cgcaatcgcg ccctctggca gtcgcatccc gccggcgaat
     1621 ccgagctcgc cgaatggttc agccaagccg gcctggatta ttcccaattt cccgaaacgc
     1681 accgcctgct tgaggccttg cgccaggagg gcccgcccct gcccgagtct tcgccgtggt
     1741 ggtttttgcg ggaccggctg atttccggcg atcaattcat cgcctacgtg cggccggcta
     1801 aaaaccggcc cttcaccgaa gcgctgttcg gcccgctggc acgggaaacc ctggtcaccg
     1861 gctggagcac gctgctggga agcctcgtgc cctgggccca gcgggagttg atcgtttacg
     1921 gcggcgcggt ggccgggatc atcctcctcg tgctggcggc gatttatcgc gacgcgcggg
     1981 tctggggcgt tcacgtgctc ggcctgattt tcgcgtatgg cgggctggcc gcgacgctga
     2041 agctgctggg cgtgaaactg aatctcctca acgtcctcgc ctttccgctc atcctcggca
     2101 ccggggtgga ttacggcacg cacctgctgc tcacgctccg ccacggggaa aaatggcggg
     2161 agcatctcac gctggtgttc cggcccattc tcttcggcgc gctgaccacc gtcgcgggct
     2221 tcgccgccct gatcccggct tccagtcccg cgctcagcgg cctcggcctc gtctgcgccc
     2281 tcggcgtggc gtggaccttg atctccgcgc tctttttcat cctgcccgcc atggcttgga
     2341 tgggccgggc gaaccggcct cggcctgccc ggccgccgtc gccgccgtca ctcttgctaa
     2401 tagccgttgc cattctctcc atcgtgctcc cctccccaag cgccaatgcc gatgacgcgc
     2461 ttcgcctggc gcagatcgaa ct
//
LOCUS       DATDAY010000232         2665 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_435656, whole genome shotgun sequence.
ACCESSION   DATDAY010000232 DATDAY010000000
VERSION     DATDAY010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2665)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2665)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2665
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_435656"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..562
                     /locus_tag="VIS74_04610"
     CDS             <1..562
                     /locus_tag="VIS74_04610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyl transferase family 2"
                     /protein_id="HEY5792555.1"
                     /translation="LLLFLLCIIMLPHSVAKHPGTIYSLLVDLPIFLITTVSIGLFYI
                     IAQRHLSPQNWLQKLLLVPLLLSLAIGMSINNARAVLEALFGHQSGFMRTPKYGDGSG
                     AQAAAPKRRFSLNSLLSVFELMFGLYFLYCATNAILNHQWTSVPFLLLFLFGFFYVFT
                     QSMGMSLRPLPPNRRGSNEDPEAVAA"
     gene            complement(604..1488)
                     /locus_tag="VIS74_04615"
     CDS             complement(604..1488)
                     /locus_tag="VIS74_04615"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEY5792556.1"
                     /translation="MTPESEKKVQTKVGIFVLIGLLVAASMVTYFGRLGEGLKRYYAL
                     RVEYENASGLLGGANVLMSGAKVGRTATGPQILPGLDGVYVDLKIYDYVKIPEGSVFT
                     IGSSGLLGDRFVNIELPPNARERAPIGPDATVKGKRESGISEFMEQGGDVVAEVKEVL
                     QKINVVISRLDAELLKSDTLKDLGDSIANLKTTTATLAESSKKVNGILDSTGGKLDGL
                     MTDASATMKSARATADELKGAVADAQTVIQNVKRGRGALGMLLSNQEFADNLRNFVAN
                     LRQSGVLFYRDRSQRREN"
     gene            complement(1500..2252)
                     /locus_tag="VIS74_04620"
     CDS             complement(1500..2252)
                     /locus_tag="VIS74_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY5792557.1"
                     /translation="MNKPLIQVRNLVQRIGAQEILKGLSLEIYAGETLVLLGKSGGGK
                     SVFLRHLIGLMKPVSGEILFEGRNIESLSERALEPVRRKIGMVFQDGALFDSMTVFDN
                     VAFPLRERGEKNEARIREKVVETLSRVNMNGHESKMPVNLSGGMRKRVSLARAIISPP
                     DVILYDEPTAGLDPIVSDSINRLIRRMQRTLKLTSVIVTHDMTSAYHVGDRVALLNEG
                     RIHFYGTLEELRGTKDAIVRDFVEGRSQDTDL"
     gene            complement(2245..>2665)
                     /locus_tag="VIS74_04625"
     CDS             complement(2245..>2665)
                     /locus_tag="VIS74_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY5792558.1"
                     /translation="QIDALRALAVHPTDYLVVPRVLAMMISMPLLVAECIFFGILAGF
                     TMATKVLGVNAAYYLENMYRFTQGEDILMGLTKGFCFAVIIVIVSCREGLLAKDGAVG
                     VGRATTEAVVISSLAVLVANFFLTMALNIPFPAGTNE"
BASE COUNT          498 a          970 c          666 g          531 t
ORIGIN      
        1 cctcctgctc ttcctgctct gcatcatcat gctcccgcat tccgtcgcga agcacccggg
       61 caccatttat tccctgttgg tggatctgcc catcttcctg atcaccacgg tttccatcgg
      121 cctgttttat atcatcgccc agcggcatct ttctccccaa aactggctgc aaaaactgct
      181 gctcgtcccg ctgctgcttt ccctcgccat cggcatgtcc atcaacaatg cccgggccgt
      241 gctcgaggcc ctcttcggcc accagagcgg cttcatgcgc actcccaaat acggagacgg
      301 aagcggcgcc caggcggccg cccccaaacg ccggttctcg ctcaactccc tcctgtcggt
      361 gtttgaactg atgttcgggc tttatttcct ctactgcgcc accaacgcga tcctgaacca
      421 tcaatggact tccgtgccat tcctgctgct ctttctcttc gggttcttct acgtgttcac
      481 ccaatccatg ggaatgtccc tgcgcccgct cccgccgaac cggcgcggtt cgaacgagga
      541 tcccgaagcc gtggcggcat gacaagccgc tagcggcggc caatggccat cgcaaccaac
      601 caatcagttc tcccgccgct ggctccggtc gcggtaaaac aaaactccgc tctgccggag
      661 attcgcgaca aagttccgca gattgtccgc aaattcctga ttcgagagca gcattcccaa
      721 cgcgccccgc ccgcgcttga cgttctggat gaccgtctgc gcgtccgcca ccgccccctt
      781 taattcatcc gccgtcgccc gcgccgattt catcgtcgcc gaggcgtccg tcatcaggcc
      841 gtccagcttg ccgccggtgg aatcgaggat gccgttcacc ttcttcgagg attccgccag
      901 cgtcgccgtg gtcgtcttga gattcgcaat ggaatcgccc agatccttga gcgtgtcgct
      961 cttgagcaac tcggcgtcga gccgcgagat cacgacattg attttctgga gcacctcctt
     1021 gacctcggcc accacatcgc ccccctgctc catgaattca ctgatgcccg actcccgctt
     1081 gcccttcacc gtggcatccg gcccgatcgg cgcgcgctcg cgcgcgttcg gcggcagctc
     1141 gatgttcaca aaccggtcgc ccagcagccc ggagctgcca atcgtaaaca ccgaaccttc
     1201 gggaattttc acgtaatcgt aaattttcag atccacataa accccatcca gtccgggcag
     1261 gatttgcggt ccggtggcgg tccggccgac cttcgcgccg gacataagca cattcgcccc
     1321 gcccagcagg ccgctcgcgt tttcatactc cacccgcagc gcgtaatagc gcttcaaccc
     1381 ctcccccagc cggccgaagt aggtcaccat cgatgccgcg accaagaggc cgatcagcac
     1441 aaaaatgccg accttcgttt ggactttttt ctcactctcc ggtgtcataa tggctcccct
     1501 taaaggtcgg tatcctgcga acgcccctcc acaaagtcac gcacgattgc atccttcgtg
     1561 ccgcgcaact cctccagcgt gccgtaaaaa tggatgcgcc cctcgttgag cagcgccacg
     1621 cggtcgccga cgtgatacgc gctcgtcatg tcgtgcgtga cgatcaccga cgtcagcttc
     1681 agcgtccgct gcatgcggcg gatgaggcgg ttgatgctgt ccgacacgat cggatccagc
     1741 cccgccgtcg gctcgtcgta aagaatcaca tccggcgggg aaatgatcgc ccgcgccagc
     1801 gagacgcgct tgcgcatgcc gccgctcagg ttcaccggca tcttggattc atggccgttc
     1861 atgttcaccc gcgaaagcgt ctcgaccacc ttctcgcgga tgcgcgcctc attcttttcc
     1921 ccccgctccc gcagcggaaa cgccacgttg tcaaagaccg tcatcgaatc aaacagcgcg
     1981 ccgtcctgga acaccatgcc gatcttccgc cgcaccggct ccagcgcgcg ctcgctgagg
     2041 gattcgatgt tgcgcccctc gaaaagaatc tccccggaaa ccggcttcat caacccgatc
     2101 aggtggcgca ggaacacgct cttgcccccg ccgcttttgc ccagcagcac cagcgtctcg
     2161 ccggcatata tctccagcga cagccccttg aggatttcct gcgcgccgat gcgctggacg
     2221 agattccgaa cctggatgag cggcttattc attcgtgccc gccgggaagg gaatgttcag
     2281 cgccatcgtc aggaaaaaat tcgccaccag caccgcgagc gacgagatca ccaccgcctc
     2341 cgtcgtcgcg cggcccacac ccaccgcgcc atccttcgcc agcagcccct cccggcagct
     2401 cacgatgaca atgatcaccg cgaaacagaa ccccttcgtc aaccccatga gaatgtcctc
     2461 cccctgggtg aagcgataca tgttttccag gtaatacgcc gcattcacgc ccagcacctt
     2521 cgtcgccatg gtgaatccgg ccaggatgcc gaaaaagatg cactccgcca ccagcagcgg
     2581 catggaaatc atcatcgcca gcacgcgcgg gaccacgaga taatccgtgg gatgcaccgc
     2641 cagcgcccgc agcgcgtcga tctgc
//
LOCUS       DATDAY010000233         2142 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_2006018, whole genome shotgun sequence.
ACCESSION   DATDAY010000233 DATDAY010000000
VERSION     DATDAY010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2142)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2142
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2006018"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..350
                     /locus_tag="VIS74_04630"
     CDS             <1..350
                     /locus_tag="VIS74_04630"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY5792559.1"
                     /translation="GAIVNNASVLGIRPLANTAIYNASKSAIIGLTKSAALEFAAQGV
                     RVNVVCPAVIETDMTSLIRDNEALNQHMKALHPVGRFGKASEVAAAVLYLCSEGAEFS
                     TGVVLTVDGGYAM"
     gene            381..1205
                     /locus_tag="VIS74_04635"
     CDS             381..1205
                     /locus_tag="VIS74_04635"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY5792560.1"
                     /translation="MSTITTKDGTQIYYKDWGTGQPIVFSHGWPLTADAWESQMFFLA
                     SHGYRCIGHDRRGHGRSSQPWEGNHMDQYADDLAELFEHLDLKDAVMIGHSTGGGEVA
                     RYIGRHGTKRVSKAVLMGAVPPLMVKTEKNPGGLPIEVFDGFRAAYLKDRSQFFLNVA
                     GGPFFNFNRPGAEVSPGLIQSWWMQGMMSGHKNAYDCIKAFSETDFTEDLKKFDVPAL
                     IIHGDDDQIVPIGASAMLSSKLVPNAILKIYPGGSHSLGDTAKDQLNADLLEFIQS"
     gene            1225..1710
                     /locus_tag="VIS74_04640"
     CDS             1225..1710
                     /locus_tag="VIS74_04640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792561.1"
                     /translation="MELSVFTLVHILISLAGILSGLVVLGGWLAGKNYRGLTSFFLAT
                     TALTSVTGFCFPFRGFTPAYAVGILSVILLAAAYYALYAGKLAGAWRRVFVINALVAL
                     YLNFFVLVAQIFQKTPALKELAPTQSEPSFAIAQTLVLVAFAALGIAAVRNFKAMPPS
                     A"
     gene            1858..2028
                     /locus_tag="VIS74_04645"
     CDS             1858..2028
                     /locus_tag="VIS74_04645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792562.1"
                     /translation="MNLGSTIRSIALDVNYTMYNNALMDVCFVELEVFTLEVMRVADE
                     DTLRQFQIELAA"
BASE COUNT          414 a          639 c          633 g          456 t
ORIGIN      
        1 gcggcgcgat cgtgaacaac gcctccgtgc tgggcatccg tccgctcgcg aacaccgcca
       61 tctacaatgc cagcaaatcc gccatcatcg gcctcacgaa gagcgcggcg ctggagttcg
      121 ccgcgcaggg cgtgcgcgtg aatgtcgtgt gtccggccgt gatcgagacg gacatgacga
      181 gcctgatccg tgacaacgaa gccttgaacc agcacatgaa ggcgcttcat cccgtgggcc
      241 gcttcggcaa ggcgtccgaa gtcgcggccg ccgtgcttta cctctgctcg gagggcgcgg
      301 aattctcgac cggcgtcgtg ctcaccgttg atggcggata cgcgatgtga acacgagctt
      361 ttcactcacc caataaacgc atgagcacga tcaccaccaa ggacggcacg cagatttatt
      421 acaaggactg gggaacgggg cagcccatcg tcttcagcca cggctggccg ctgacggcgg
      481 atgcctggga gtcgcagatg ttttttctgg cgtcccacgg gtatcgctgc atcgggcatg
      541 atcgccgcgg gcatggccgc tcgagccagc cctgggaggg aaaccacatg gaccaatatg
      601 cggacgacct ggcggaactc ttcgagcatc tcgacttgaa ggacgcggtg atgatcggcc
      661 attcgaccgg gggcggggag gtggcccgct acatcggccg ccacggcaca aaacgggtga
      721 gcaaggcggt gctcatgggc gcggtgccgc cgcttatggt gaagaccgag aaaaacccgg
      781 gcgggttgcc gatcgaggtc ttcgacggat tccgcgcggc ttatctcaag gatcgctcgc
      841 agtttttcct gaacgtggcc ggcggcccat ttttcaactt caaccggccc ggcgcggagg
      901 tttccccggg gctgatccaa tcctggtgga tgcaggggat gatgtccggg cacaaaaacg
      961 cctacgattg catcaaggcg ttttccgaaa cggatttcac cgaagatttg aagaaattcg
     1021 acgtgccggc gttgatcatt cacggcgacg acgaccagat cgtgccaatc ggcgcgtcgg
     1081 ctatgctctc gtcgaagctc gttcccaacg ccatcctgaa gatctatccc ggcggttcgc
     1141 acagccttgg ggataccgcc aaggaccagc tcaacgccga cctgctcgaa tttatccaat
     1201 cgtaacctcg gaagggaaaa aaccatggaa ctgtccgtct tcaccctcgt tcacatcctg
     1261 atcagcctgg cgggcatcct ttcgggcctc gtcgtccttg gcggctggct cgccgggaaa
     1321 aattaccgcg ggctcacgtc attcttcctc gccaccacgg ccctcaccag cgtgacggga
     1381 ttctgttttc ccttccgcgg cttcactccg gcctatgccg tggggattct ctccgtgatc
     1441 ctgctggctg cggcgtatta tgcgctttac gccggcaagc tcgccggggc gtggcgccgg
     1501 gttttcgtaa tcaacgccct cgtcgcgctt tacctgaatt tctttgtgct ggtcgcccag
     1561 attttccaaa agacgccggc gctcaaggaa ctggcgccga cgcagagcga gccgtcgttt
     1621 gccatcgcgc aaacgctcgt gctcgtggca tttgccgccc tgggcatcgc cgccgtgcgt
     1681 aatttcaagg cgatgccccc ctcggcttaa aaggaatttc gtttcgagta tcttcaagcc
     1741 ctgcgtgtgc tgaatagtga acaccgtcag ctgtagcggc agtctatggc cgtcggttcc
     1801 ctgatcctca gctcccctcg acggcgctct tattttccgt gaagcgcgcc cgaaggtatg
     1861 aaccttggtt cgaccatccg ctcgatagcg cttgacgtga attatacaat gtataataat
     1921 gccttgatgg atgtctgttt tgtcgagctt gaagtcttta cgctggaagt gatgcgtgtg
     1981 gccgatgaag acaccttgcg acagttccaa atcgaacttg cggcttaatc ctgagagcgg
     2041 tgatttgatc cggcaatcgg gaggattgcg caaggctcgg atgaaattgc ccggtcgcgg
     2101 cagaagttcg ggagcgcggg tcatttaaaa atccagaaat tc
//
LOCUS       DATDAY010000234         2445 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_206550, whole genome shotgun sequence.
ACCESSION   DATDAY010000234 DATDAY010000000
VERSION     DATDAY010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2445)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2445)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2445
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_206550"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..777
                     /locus_tag="VIS74_04650"
     CDS             <1..777
                     /locus_tag="VIS74_04650"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HEY5792563.1"
                     /translation="RVEEVKSWHDGCAYLAGGTWLFSEPQVHLRTLIDLDTLKWPPLT
                     ATAGGLEIAATCKVVDLDRSTAPPEWLAAPLLSECCHSFLASFKIWNEATVGGNLIMS
                     LPAGPMISLTAALEGVMTLWPRGGAPREVRVADFVTGNHQNDLQPGELVRSILLPAHA
                     LKKKYAFRRFTLTHLGRSETLVIGTLCPEHGEFLLTVTASTLRPWQFHFEGLPTAAEV
                     RDAIDGIPLDQYLADTHGSPEHRQHLTYYFSEQIRQELSA"
     gene            786..>2445
                     /locus_tag="VIS74_04655"
     CDS             786..>2445
                     /locus_tag="VIS74_04655"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEY5792564.1"
                     /translation="MSYKINGKTFDAEPRPGQCLRTFARDLGWFGVKKGCDAGDCGAC
                     TVWLDGKPVHSCLVPAFRAAGREVTTIEGLACNGSLHPMQQAFLDAQAFQCGFCAAGM
                     IMTSASLSEEEKKDLPFRLKGNLCRCTGYHAIEDALGGIGSVEEDRAGHACGASLQNP
                     AARDIVTGQARYTADVKMEGMIHLKVLRSPHAHAKITAIRTDKARALPGVHAVFTFED
                     APKRPYTSALHDDYRVDPDDTYVLDDVVRHVGQRVAAVAADTEGIAEAACRLIEVDYE
                     VLPAVFDPEEAMRPGAPILHQKEFSSRIQQAERNIFLHIESEYGSVEKGFAEADVTVD
                     GAYYSNKISHAHLETHIAIASQSEDGRIHVRTSSQAPFQTKAKLAYLFGIPLQQVHVY
                     TERVGGGFGGKQEMLCEELCVLATLKTGRPVKWEFTREEEFIAGVSRHPMKVEIKAGA
                     KRDGTLTAIQVRVVSATGAYGNHGGETLAASLSQSLQVYRCPNMKGSGFAVYTNIPPS
                     GAVRGYGSSQTTFPVESAMGDLARKLGMDLMTLQRKNVVVPGDAV"
BASE COUNT          432 a          862 c          751 g          400 t
ORIGIN      
        1 cgcgtggagg aggtgaaatc ctggcacgac ggctgcgcct accttgcggg gggcacgtgg
       61 cttttttccg agccgcaggt ccacctccgc accctgatcg acctcgatac cctcaagtgg
      121 cccccgctca ccgccacggc cggggggctg gagatcgccg ccacctgcaa agtggtcgat
      181 ctcgaccgct ccaccgcgcc gcccgagtgg ctggccgcgc cgctgcttag cgaatgctgc
      241 cattccttcc tcgcctcctt caaaatctgg aacgaggcca ccgtcggcgg caatctcatc
      301 atgtcgcttc cggccgggcc gatgatctcg ctcaccgccg cgctggaagg cgtgatgacc
      361 ctctggcccc gcggcggcgc cccgcgcgag gtgagggtgg cggactttgt gaccggcaat
      421 caccaaaacg atctccagcc cggagagctg gtgcgctcca tcctcctgcc ggcgcacgcg
      481 ctgaaaaaga aatacgcctt ccgccggttt acccttacgc atctcgggcg ttcggaaacg
      541 ctggtcatcg gcacgctttg cccggagcac ggggagttcc tcctcaccgt cacggcctcg
      601 accctgcggc cctggcaatt ccatttcgag ggccttccca ccgccgcgga ggtgcgggac
      661 gccattgatg gcatcccgct cgaccaatac ctcgccgaca cccacggctc gccggaacac
      721 cgccagcacc ttacctatta tttctcggaa caaatccgcc aggaactctc cgcctaaccc
      781 ccattatgag ctacaaaatc aacgggaaaa ctttcgacgc cgagccccgg cccggccaat
      841 gcctccgcac cttcgcccgg gatctcggat ggttcggcgt gaaaaaaggc tgcgacgccg
      901 gggattgcgg cgcttgcacg gtctggctcg acggcaagcc cgtccacagc tgcctcgtgc
      961 ccgccttccg ggccgcgggc cgcgaggtca ccaccatcga gggcctggcc tgcaacggca
     1021 gcctgcatcc catgcagcag gctttcctgg acgcccaggc gtttcagtgc ggcttttgcg
     1081 cggcgggcat gatcatgacc tccgccagcc tgagcgagga ggagaagaag gatctgccct
     1141 tccgcctcaa gggcaacctc tgccgctgca ccgggtatca cgcaatcgaa gacgcgctcg
     1201 gcggcatcgg ctcggtcgag gaagaccgcg cggggcacgc ctgcggcgcc agtctgcaga
     1261 atcccgcggc ccgcgacatc gtcaccggcc aggcgcgcta caccgccgac gtgaagatgg
     1321 agggaatgat ccacctcaag gtgctgcgct cgccccacgc tcacgcgaaa atcacggcga
     1381 tccgcacgga caaggcgcgc gccctgccgg gcgtgcacgc ggtgttcacc ttcgaggatg
     1441 cgccgaagcg gccttacacc tcggcgttgc acgacgacta ccgcgtggat cccgatgaca
     1501 cgtatgtgct cgacgacgtc gtccgtcacg tcggccagcg cgtcgcggcg gtggccgcgg
     1561 atacggaggg aatcgccgag gcggcctgcc gcctgatcga ggtggactac gaggtgctgc
     1621 cggcggtgtt cgatcccgag gaagcgatgc ggccgggcgc tccgatcctg caccaaaagg
     1681 aattttcgtc gcgcatccag caggcggagc ggaatatttt cctccacatc gagagcgaat
     1741 acggcagcgt ggagaagggt ttcgccgagg ccgacgtcac cgtggatggc gcctactaca
     1801 gcaacaagat ctcgcacgcg catctcgaga cccatatcgc catcgcctcg cagagcgagg
     1861 atgggcgcat tcacgtccgc accagttcgc aggcgccttt ccagaccaag gccaagctgg
     1921 cttacctttt cggcattccg ctccagcagg tgcacgttta caccgagcgc gtcggcggcg
     1981 gtttcggcgg caagcaggag atgctctgcg aggagctctg cgtgctggcc acgctgaaga
     2041 ccggccggcc ggtgaagtgg gaattcaccc gcgaggagga attcatcgcc ggcgtttcgc
     2101 ggcacccgat gaaggtcgaa atcaaggccg gcgcaaagcg ggatggcacc ctgacggcga
     2161 tccaggttcg cgtcgtctcc gccaccggcg cctacggcaa ccatggcggc gaaaccctgg
     2221 ccgcctccct cagccagtcg ctccaggttt accgctgccc gaacatgaaa ggcagcggct
     2281 ttgccgttta cacgaatatc cccccctccg gcgccgtccg cggctacgga tcttcgcaga
     2341 cgaccttccc cgtggaatcc gccatgggcg atctcgcccg caagctcggc atggatctca
     2401 tgaccctgca gcgcaagaac gtcgtcgtgc ccggcgacgc cgtgc
//
LOCUS       DATDAY010000235         6618 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_2354529, whole genome shotgun sequence.
ACCESSION   DATDAY010000235 DATDAY010000000
VERSION     DATDAY010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 6618)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6618)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6618
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2354529"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1047
                     /locus_tag="VIS74_04660"
     CDS             <1..1047
                     /locus_tag="VIS74_04660"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing SpoIIE family protein
                     phosphatase"
                     /protein_id="HEY5792565.1"
                     /translation="RLQSITRGEGAVGQAWQAGEALLLHEKELHSAESRASPLTAFSA
                     LISPLSYRRKTLGVLALANGPMSMAFTEEDLNLFRSISEQSAFALYNEVVYNEASEKR
                     KLDHDLEIAREIQAVLLPNTSPDIPGFEISGFNLPARRVSGDYYDYIPVDESHLGVAI
                     ADVSGKGVPASLIMTMCRSVIRSQAPHHFSPAEVLSRVNRQLYPDIREDMFISMAYIL
                     LNRNSSQVLMARAGHDAPLLYRAATKEVETLNPKGMALGIDSGSVFDRVCTDFRFTLE
                     RGDCLLLYTDGATEAVDSNGLEFGIPRLVQGLQASAPDGVRHVIERLSGDVKNFAGNN
                     VQYDDVTLIAIRKL"
     gene            1044..1574
                     /gene="grpE"
                     /locus_tag="VIS74_04665"
     CDS             1044..1574
                     /gene="grpE"
                     /locus_tag="VIS74_04665"
                     /inference="COORDINATES: protein motif:HMM:NF010738.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEY5792566.1"
                     /translation="MNAKPDQESILEEETPATPEAAKPPAPSAEAEIAKYRDLALRSQ
                     AELDNFRKRVIREKEDAIRYANLSLLERLLPVVDNFELGLEAAKTATDANSIALGMSM
                     VQKQLLDFLKDSGAEVIATEGAPFDPNLHEAVSHQAHATVPDGRIVQQLRKGYKLKDR
                     LLRPATVVVSKGPGES"
     gene            1571..2713
                     /gene="dnaJ"
                     /locus_tag="VIS74_04670"
     CDS             1571..2713
                     /gene="dnaJ"
                     /locus_tag="VIS74_04670"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEY5792567.1"
                     /translation="MTAKRDYYEILLVERTAEADEIKRSYRKLAVKFHPDKNPGDKDA
                     EEKFKELGEAYDVLMDPEKRAAYDRYGHAAFQQAGGRGAAGGGFHDPFDLFREVFGGG
                     GGGGGIFEQFFGGGADSSGRQRGSDLRYDLQITLEEAASGCEREIEIRKLDTCESCHG
                     SGAQKGSNVVTCSTCRGRGQVVSSRGFFQVAQTCPHCQGTGRVVEKPCKTCAGEGRHE
                     KTSRLKIKIPAGIEEGSRLRSAGAGEAGIRGGTPGDLYVVVHVKQHRLFERDGHDLHC
                     EIPLPFTAAALGGEIQVPTLSGAVNLKIPAGTQSGAVFRIRGNGMPALGSAARGDLYA
                     KVAVEVPTRLNAQQREKLQEFAELCGDDNTPLHRSFYERLKDLFHG"
     gene            2849..4246
                     /locus_tag="VIS74_04675"
     CDS             2849..4246
                     /locus_tag="VIS74_04675"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEY5792568.1"
                     /translation="MLLSCEEMKRAEAAAFARGVRVEDLMEPAGRGIAEVIRQFQPHP
                     GTCVVFAGKGNNGGDARIAARHLEAWGWKIREPGFELGRGDRPLIIIDGLLGIGTRGA
                     PREPVASAIRQINALRKNRSARVFAVDLPTGLDGGSGIPADPCVQADVTITLGQPKTC
                     LVQDVAANHVGRIAFVPLPELTATEGNPAQLSCPETLRSFLPPRDFDSHKGTHGRIGL
                     IAGSEGFLGAARLCSAAALHGGAGLVTLFARREIYPSLAGTVAPEIMVQPVDHYGQAL
                     EFRLNVLALGPGLGRWHDAEALDLIEKAHLPLVVDADALNALSTHLDRLKNSRGPRLL
                     TPHLGEMARLFPEGAETRREAAETFTGSHPVTLLMKSSRSLVAEQGKVLFYNPTGNPG
                     MGSGGMGDVLTGVCAALLGQGLAPAPAAAVGAWVCGRAAEIACQYAESEESLSAAHVI
                     SHLGLAFRSLREGDY"
     gene            complement(4289..5074)
                     /locus_tag="VIS74_04680"
     CDS             complement(4289..5074)
                     /locus_tag="VIS74_04680"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY5792569.1"
                     /translation="MNSYNHTQKWEIEFLESEEVLRSKNFGITAQAKKVRYPVRLLRY
                     WFGYHLLAEECRRAGRPIDVAEIGIHNGQMLLFAKLAASKVKDPAQVLQFASWLGVDA
                     VLKHDRLAKAGYTELQEANLEDADFQIAQPCDTAICLHVFEHLSDPEEVLRKIALSVR
                     PGGSVIGGFPVLPHFLIGMRERQVRKTAQPMGHVSIFSPQRVKSMAESAGLKLEFSAG
                     AFFMRRKGSAIENSPSWLKFNLRWGETFPWWPGEIYWLARKEK"
     gene            complement(5071..5568)
                     /gene="lspA"
                     /locus_tag="VIS74_04685"
     CDS             complement(5071..5568)
                     /gene="lspA"
                     /locus_tag="VIS74_04685"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEY5792570.1"
                     /translation="MPIENRKSKIENARLLLALTLPLYALDQLTKWLVLRFIAEDEFI
                     PVIPNFFNLIRTYNTGAAFSIGQNNNGFFLVLAAAVLAVLLALWFRGKFQQRLTRAAV
                     ALLLAGIAGNLTDRLIHGHVIDFLDVILPWYGHWPAFNVADSCICVAAGLLLIGALKE
                     PKNRS"
     gene            complement(5568..>6618)
                     /locus_tag="VIS74_04690"
     CDS             complement(5568..>6618)
                     /locus_tag="VIS74_04690"
                     /inference="COORDINATES: protein motif:HMM:NF019869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEY5792571.1"
                     /translation="SLMTSVALNDCAPYREVITHGFVVDKDGKKISKSAEYKKPTHAE
                     HFVNQYGADILRLWVATVNYTDEVPFGEDVFKQVAERYRGFRNIQRILLANLFDFAPA
                     NASLDGATAIDRWVLSRLREVTAKCRAAYEAYEFRAVFEALNQFCTVDLSALYIDITK
                     DRLYCDAIDSPRRRATQTVMHACFDSITRLLAPILVFTAEEAWDYFKPGSSIHLEQFP
                     EVNAELDNEEVRQTGKRWLEWRGRISQAVEQAQKNGEIANPLEARVRVRVKDAAEAEL
                     LQSRVAELEEFWILSHLEIVPGDEDSVEVAKTSQVKCERCWRHRPDVGANAEHPTLCG
                     RCAEAVLSRVLTAAS"
BASE COUNT         1282 a         2119 c         2031 g         1186 t
ORIGIN      
        1 cggcttcaaa gcatcacgcg cggggagggc gccgttggcc aggcctggca ggccggagag
       61 gcgctgctcc tgcatgaaaa ggaactccat tccgcggaaa gccgcgcctc cccgctcacc
      121 gctttctcgg ccctgatcag cccgctcagc taccggcgca agacccttgg cgtcctcgcc
      181 ctggccaacg gccccatgag catggctttc accgaggagg atctgaatct cttccgcagc
      241 atctccgaac agtccgcctt cgccctctac aacgaggtcg tttacaatga ggccagcgaa
      301 aaacgcaaac ttgaccacga cctcgaaatc gcccgcgaaa tccaggccgt cctgctgcca
      361 aacacctcgc ccgatattcc gggttttgag atcagcgggt ttaatctgcc cgcgcgccgg
      421 gtcagcgggg attattacga ttacattccc gtggatgaat cccacctggg cgtggccatt
      481 gccgacgttt cgggcaaggg cgtgcccgcc tcgctgatca tgaccatgtg ccggagcgtc
      541 attcgcagcc aggcccccca ccatttttcc ccggccgaag tgctcagccg ggtgaaccgc
      601 cagctctacc ccgacatccg cgaggacatg ttcatcagca tggcttacat tctcctcaac
      661 cggaattcct cccaggtttt gatggcccgc gccggccacg acgcccccct gctctaccgc
      721 gccgcgacga aggaagtcga gacgctcaat cccaagggca tggcgctggg cattgacagc
      781 ggttcggtct tcgatagggt atgcaccgac ttccgattca ctttggaacg cggggactgc
      841 ctgctgctct acaccgacgg ggcgaccgaa gccgtggatt caaatggatt ggagttcggc
      901 attccgcggc tcgtgcaggg cctgcaagcc agcgcacccg acggagtccg gcatgtcatc
      961 gaacgccttt caggcgacgt caaaaacttc gcgggcaata atgtccaata tgatgacgtc
     1021 accctgatcg cgatccgaaa attatgaacg cgaaacccga ccaagaatcc attttagaag
     1081 aagaaacccc cgcaaccccg gaagcggcca aaccgccggc tccgtccgcg gaggcggaaa
     1141 tcgcgaaata ccgcgacctc gcgctgcgca gccaggcgga actcgacaat ttccgcaagc
     1201 gcgtgatccg cgagaaggag gacgccattc gctacgcgaa cctctcgctg ctcgagcgcc
     1261 tcttgcccgt cgtggacaac ttcgaactcg gactggaagc ggccaagacc gccacggatg
     1321 cgaactccat cgccctcggc atgagcatgg tccagaagca gctcctggat tttctcaagg
     1381 acagcggcgc ggaagtcatc gccacggaag gcgcgccgtt cgatcccaac ctgcacgagg
     1441 ccgtcagcca tcaagcgcac gccaccgtgc cggacggccg catcgtccag cagctccgca
     1501 agggctacaa gctcaaggac cgcctgcttc gtccggcgac cgtggttgtc tccaaggggc
     1561 cgggtgaatc atgacggcca agcgcgacta ctacgaaatc ctcctcgtgg agcgaaccgc
     1621 ggaggccgac gaaatcaagc ggtcctaccg caagctcgcg gtcaaattcc atcccgacaa
     1681 aaacccgggc gacaaggatg cggaggaaaa attcaaggaa ctcggcgagg cctacgacgt
     1741 cctcatggac ccggagaaac gcgccgcgta tgaccgctac ggccacgccg cctttcaaca
     1801 ggccggcggc cgcggcgccg cgggcggcgg attccacgat cccttcgatc tcttccgcga
     1861 agtcttcggc ggcggggggg gcggcggcgg catcttcgaa caatttttcg gcgggggcgc
     1921 cgacagctcg ggccggcagc ggggttccga cttgcgttac gatctccaga tcacgctgga
     1981 ggaagccgcc agcggctgcg agcgcgagat cgaaatccgc aagctcgaca cctgcgagtc
     2041 ctgccatggc agcggcgcgc agaagggctc caatgtcgtc acctgctcga cctgccgcgg
     2101 ccgcggccag gtggtcagct cccgcggatt tttccaggtc gcgcagacct gcccgcattg
     2161 ccagggaacc ggccgcgtgg tcgagaaacc gtgcaagacc tgcgcgggcg aaggccggca
     2221 cgaaaagaca tcgcgcctga agatcaaaat tcccgccggc atcgaggaag gctcgcgcct
     2281 gcgctccgcc ggagcgggcg aggccggcat ccggggcggc acgccgggcg atctctacgt
     2341 ggttgtccac gtcaaacagc accgcctctt cgaacgcgac gggcacgacc tccattgcga
     2401 gattcccctg cccttcaccg ccgccgcgct cggcggggaa atccaggtgc ccacgctttc
     2461 cggcgcggtg aatctgaaaa ttcccgccgg cacccagagc ggcgcggtct tccggattcg
     2521 cggcaatggc atgcccgccc tcggcagcgc ggcccggggc gatctctacg ccaaggttgc
     2581 cgtcgaagtg ccgacgcggc tcaatgccca acagcgcgag aaattgcagg aattcgccga
     2641 actctgcggc gacgacaaca cgccgctgca ccggtccttt tacgagcgcc tgaaggattt
     2701 gtttcacggt taggaccgga acgattttta accgcaagga acgcggagaa cgcaaaaatc
     2761 cagggccatt ctttgcgtgc ttcgcggtta aggcttttcc gcgattttga agtaggcacg
     2821 cgcggcgcgg ctgccttagc attcacgcat gcttctaagc tgtgaagaga tgaagcgggc
     2881 cgaggcggcc gcctttgccc gcggagttcg ggtggaggac ttgatggagc cggccggccg
     2941 gggcatcgcc gaggtcatcc ggcagtttca accccatccc ggcacctgcg tcgttttcgc
     3001 cggcaagggc aacaacggcg gcgatgcccg gattgccgcg cggcacctgg aagcgtgggg
     3061 ctggaaaatc cgcgaacccg gattcgaact ggggcgcggc gaccgcccat tgatcatcat
     3121 tgatggcctg ctgggcatcg gcacccgcgg cgctccccgg gaaccggtgg cttcggcgat
     3181 tcggcagatc aatgcgctgc ggaaaaaccg aagcgcccgt gtcttcgcgg tggaccttcc
     3241 caccgggctc gatggcggga gcggaattcc cgccgatccc tgcgtgcagg cggatgtcac
     3301 catcactctc ggccagccga agacctgcct cgttcaggac gtggcggcga atcacgtggg
     3361 gcggatcgcc ttcgtgcccc tgccggaatt gacggcgacc gaggggaacc ccgcgcagct
     3421 ttcctgtccg gaaaccctgc gttctttttt gcctccccgg gatttcgatt cgcacaaagg
     3481 cacccacggg cggatcgggc tcatcgcggg ttcggaaggt tttctcggcg ccgcgcgcct
     3541 ttgctccgcc gccgccttgc atgggggggc ggggctggtc acgctgtttg cgcggcgcga
     3601 gatctatccg tccctcgccg gcaccgtggc gccggagatc atggttcagc cggtcgatca
     3661 ctacggccag gcgctggaat tccggctcaa cgtgctcgcc ctggggccgg ggctgggccg
     3721 ctggcacgat gcggaagcgc ttgacctcat cgaaaaagcg cacctgccgc tggtggtgga
     3781 tgcggacgcg cttaacgccc tttccacgca cctcgaccgg cttaagaaca gccggggccc
     3841 gcgcctcttg acgccccacc tcggcgaaat ggcccgcttg tttccggaag gcgcggagac
     3901 ccggcgagag gcggcggaaa ccttcaccgg cagccatccg gtcacgctgc tgatgaaaag
     3961 ttcccgcagc cttgtcgccg aacagggaaa ggtgctgttt tacaacccga ccggaaaccc
     4021 cggcatgggc agcggcggca tgggcgacgt cctcaccgga gtctgcgccg ccttgctggg
     4081 ccagggattg gccccggcgc cggccgcggc cgtgggggcg tgggtctgcg gccgggccgc
     4141 ggaaatcgcc tgccaatacg cggagtccga ggaatccctc tccgcggctc acgtgatctc
     4201 gcatctagga ttagccttcc gcagcttgcg ggagggggat tattagtggc ttataggtcc
     4261 tatgggactt ataaggccta taagagtctt atttctcttt cctcgccagc cagtaaatct
     4321 ccccgggcca ccagggaaag gtctcgcccc accgcaggtt gaacttcagc caggacgggg
     4381 aattctcgat cgcgcttccc ttccgccgca tgaaaaatgc gccggcggaa aattcaagct
     4441 tcaggccggc ggattccgcc atggacttca cgcgctgcgg tgaaaaaata ctgacgtgcc
     4501 ccatgggctg cgcggttttg cgcacctgcc tttcccgcat tccgatcaaa aaatgcggca
     4561 gcaccggaaa tccgccgatc acggatccgc cggggcggac gctgagcgca attttgcgca
     4621 gcacctcctc ggggtcggac aaatgctcga agacgtgcag gcaaatggcg gtatcgcacg
     4681 gctgggcgat ctggaaatcc gcatcctcca gattcgcttc ctgcaactcg gtgtaacccg
     4741 ccttggccag gcggtcgtgc ttcaacacgg catccacgcc cagccagctc gcgaattgca
     4801 gcacctgcgc gggatccttg accttgctgg cggccagctt cgcaaagagc agcatctggc
     4861 cattgtgaat gccgatttcc gccacatcaa tcggccggcc ggcgcggcgg cattcctcgg
     4921 cgaggagatg gtagccgaac cagtagcgaa gcagccgcac gggatagcga accttcttgg
     4981 cttgggcggt gatgccgaaa tttttggaac gaagcacttc ctcgctttcg aggaattcga
     5041 tctcccattt ttgggtgtgg ttgtaggaat tcacgagcgg tttttcggtt cttttagagc
     5101 cccgatcagc aacagtccag cggcgacgca aatgcagctg tccgccacgt tgaaagcggg
     5161 ccaatggcca taccacggca ggatgacgtc gagaaaatca atgacgtggc cgtggatcag
     5221 gcggtcggtc agatttcccg cgatgccggc cagcagcagg gccacggcgg cgcgggtgag
     5281 gcgctgctga aatttgccgc ggaaccaaag cgccagcaaa acggccagaa ccgcggcggc
     5341 gagcacgagg aaaaaaccgt tattgttttg cccgatgctg aacgccgcgc cggtgttgta
     5401 ggtgcggatg agattgaaaa aattcggaat gaccggaatg aactcatcct ccgcgatgaa
     5461 ccgcagcacc agccatttgg tcagctgatc caaggcgtag agcggcaggg tcaatgccag
     5521 gagcagccgg gcattttcga ttttcgattt tcgattttcg attggcatca gcttgccgcg
     5581 gtcagaaccc ggctcagcac cgcctcggcg cagcggccgc acagcgtggg atgttcggcg
     5641 ttcgcgccga catcggggcg atgccgccag cagcgttcgc atttcacctg cgaggtcttg
     5701 gcaacctcca cggaatcctc atcgccggga acgatctcca gatggctcaa aatccagaac
     5761 tcctccagtt cggccacgcg ggactggagt aattccgcct cggcggcatc cttgaccctc
     5821 acccgcacgc gggcctcgag cggattggcg atttcgccat ttttctgggc ctgctccacg
     5881 gcctgggaaa tgcggccgcg ccattccagc cagcgtttgc cggtctggcg gacttcctcg
     5941 ttgtccagtt ccgcattcac ttccggaaat tgctccaaat gaatggagga accgggcttg
     6001 aaataatccc aagcctcttc ggcggtaaaa acaagaatcg gcgcgagcaa acgggtaatc
     6061 gaatcgaagc atgcatgcat caccgtctgc gtcgcgcggc ggcgcgggct gtcgatggcg
     6121 tcacaataca ggcggtcctt cgtgatgtcg atatagagcg cgctgagatc gacggtgcag
     6181 aattgattaa gcgcctcgaa aacggcgcga aactcgtaag cctcgtaggc ggcgcggcat
     6241 tttgcggtca cttcccgcaa gcggctgagc acccatcgat caatcgcggt cgctccgtca
     6301 agcgaagcgt tcgccggcgc aaagtcgaag agattcgcga gcaggatgcg ctggatgttg
     6361 cggaagccgc ggtagcgttc ggcgacctgc ttgaagacgt cctcaccgaa cggcacctca
     6421 tcggtatagt tcaccgtggc aacccacaag cgcaggatgt ccgcgccgta ttgattcacg
     6481 aaatgttcgg catgcgtggg ttttttgtat tcggcggact tggagatttt tttgccgtcc
     6541 ttatccacca cgaagccgtg cgtgatgacc tcgcggtagg gcgcgcaatc gttcagagcg
     6601 acgctcgtca tcagcgaa
//
LOCUS       DATDAY010000236         4181 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_561569, whole genome shotgun sequence.
ACCESSION   DATDAY010000236 DATDAY010000000
VERSION     DATDAY010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 4181)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4181)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4181
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_561569"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(99..1322)
                     /locus_tag="VIS74_04695"
     CDS             complement(99..1322)
                     /locus_tag="VIS74_04695"
                     /inference="COORDINATES: protein motif:HMM:NF014919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792572.1"
                     /translation="MPASQLHLHSHTPLGANLVSGGATFRTWAPAAAEVYLALNHPSG
                     TPYPKNPDELLVKDASGYWGGFFPGVKDGDLYRFYVAGTAAGPVREGFKRDPYARELE
                     MNGYPDCDCIVRDPADYQWHDQGFHPPAHSELIVYQFHIGVFYAKDSAGTDIRPHRVC
                     KILDVVDRIEYFADLGVNAVMPLPFQEYQGENSLGYNGTDLFSPEMDYSAAPADLAPY
                     LARVNRLLGNKGCAPMTAAQLTGQVNQFKTFIDLCHLHGIAVIADVVYNHAGGGFDDQ
                     SIYFFDRQPGGDNDNSLYFLRDGHAGGLVFAFWKNEVRQFLIDNGKMLLEEYHCDGLR
                     YDQVTVIDEHGGWFFCQDLTGTLRFVKASAVQIAEYWGAERWKGIAHPPAGMGFDAGY
                     SEAAQFLFCKSCELV"
     gene            complement(1331..2404)
                     /locus_tag="VIS74_04700"
     CDS             complement(1331..2404)
                     /locus_tag="VIS74_04700"
                     /inference="COORDINATES: protein motif:HMM:NF023695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3616 domain-containing protein"
                     /protein_id="HEY5792573.1"
                     /translation="MKTNPILAATTPAAAAAHAQVSAPDFPSPASAAPPLSESAPMRF
                     ASGEASIFSGACDASGVVDIGGKRFAAGTDERNTVRVFEADGNGQAVATLSLDEFLDA
                     KWDEEDQAYSECDIEGATRIGDLTLWITSHGRNSKGKEKPERRRLFALRIVPGNPGAP
                     LEPCGKPYAGLLADLLQAPELKEWHLAEAAKKPPQEKDGLNIETICATPQGGVWVGFR
                     NPIRKGKALLVEIKNPLDLVKNPSAQAKAGNGRMLDLGGRGFRDMVPWRDGYLILGGA
                     YDDKRKFALFYWKGNDETPERLPVDLDATGLSPEGLVVFPGDDRRVLILSDDDSLRFG
                     GILNKDLPEGRRTFRGRLMNLPL"
     gene            complement(2431..3915)
                     /locus_tag="VIS74_04705"
     CDS             complement(2431..3915)
                     /locus_tag="VIS74_04705"
                     /inference="COORDINATES: protein motif:HMM:NF013661.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HEY5792574.1"
                     /translation="MIWPAGPHLFIADDLIWVFTPLTTSGVHSPSWNNIAWGVELVGE
                     YDDEPFNPGVRENAVDALATLHLWRGISPDTLRFHKEDPNTTHTHCPGRNVNKADLIA
                     RIHARMASGNGGEHQPQDDYLEIGAGGGGAEPGVPALTHPPLAGNAELQLAAGGKRRP
                     FVRGRDHGEALGVIQDALDRVLPPEDRIQAGGNRGTFGPKTEAAVIRFQQSVEIEADG
                     KVGREMLLALDAALSRIDGSAPRPEPDGPAGSLDPILQIAAGSEIARYRWNERGRAPL
                     AYIKGMALVYGRVYCKFKAGDPAAVEMAKAAASTAARDVLAHYRAQFAALGMDNSVSG
                     VDTLRHLFVLLMGLGMRESSGKHCMGLYAPDGNTTGETAEAGLFQTSYNARTASPLLP
                     GIFAHYRANPSGFLEVFSQGVSCPATDAANYGSGDGKEFQRLSKQCPAFAAEFAALAM
                     RHRRNHWAPIKDHKREAEVRPEADEMLQQVEAEADRSQLGPVLV"
BASE COUNT          747 a         1371 c         1288 g          775 t
ORIGIN      
        1 cctccctcct tcgcctcatc tccgccctcc tctgcgaaat cagcgaggac tggctaaccg
       61 gaaaaattta cctcaacatg aacccggctg acctgcccct aaactaactc gcaactttta
      121 cagaataaaa attgcgccgc ctcgctgtag cccgcgtcga aacccattcc cgcgggcgga
      181 tgcgcgatgc ctttccagcg ttccgcgccc caatattcgg cgatctgcac ggcgctggcc
      241 ttcacaaaac gcagcgtgcc ggtgaggtcc tggcagaaaa accagccgcc atgctcgtcg
      301 atgacggtga cctgatcgta acgcaggcca tcgcagtgat attcttccag aagcattttc
      361 ccgttatcga tgaggaactg gcgaacctcg tttttccaaa aggcgaacac cagtccgccg
      421 gcgtggccgt cgcgcaggaa ataaaggctg ttgtcgttgt cgccccccgg ctggcggtcg
      481 aaaaagtaaa tgctttggtc gtcgaacccg ccgcctgcat gattgtaaac gacatcggcg
      541 atcaccgcga tgccgtggag gtggcagagg tcgatgaagg tcttgaattg attgacctgc
      601 cccgtcagct gggccgcggt cattggcgcg cagcctttgt ttcccaacag gcgattcacc
      661 cgcgcgagat agggggcgag gtccgccggg gcggcggaat aatccatctc cggcgagaag
      721 agatcggtgc cgttgtagcc gaggctattt tcgccctggt attcctgaaa cggcagcggc
      781 atgaccgcgt tcactcccag gtcggcgaag tattcgatcc gatcgacgac atcgagaatc
      841 ttgcagacgc ggtgcggccg gatgtcggtt cccgcgctgt ccttcgcata aaaaacgccg
      901 atgtggaatt gataaacgat caactcgctg tgcgccggtg ggtggaagcc ttgatcatgc
      961 cactggtaat cggccggatc gcgcacgatg cagtcgcagt cgggatagcc attcatctcc
     1021 agctcccgcg catagggatc ccgtttgaag ccctccctga ccggaccggc cgccgttccg
     1081 gcgacgtaaa accggtagag atcgccgtcc ttcacccccg ggaaaaagcc gccccaatat
     1141 ccggaagcgt ccttgaccag cagctcatcc ggatttttcg gataaggcgt tccactcggg
     1201 tgattcagcg caagatacac ttccgccgcg gcgggagccc aggtgcggaa ggtggccccg
     1261 ccggagacaa ggtttgctcc cagcggagta tgggaatgga ggtggagttg tgatgcaggc
     1321 atggattttt tcaaagtgga aggttcatga gccgtccgcg aaaggtgcgc cgcccctccg
     1381 gcaaatcttt gttgaggatg ccgccaaagc gaagcgagtc gtcgtcgctg aggattaata
     1441 cccggcggtc atcgccgggg aataccacca gcccctcggg ggacaacccc gttgcatcca
     1501 ggtccacggg cagccgctcg ggagtctcgt cattcccctt ccaataaaac aaggcgaact
     1561 tccgcttgtc gtcataagct ccccccagaa tcaaataccc gtccctccat ggcaccatgt
     1621 cccggaaacc gcgcccgccg agatccagca tccgcccgtt tcccgctttc gcttgcgcgg
     1681 aggggttctt taccaggtcc aggggatttt tgatctccac cagcaatgcc ttccccttgc
     1741 gaatcggatt gcggaagccc acccacaccc cgccttgggg cgtggcgcag atcgtttcga
     1801 tattgaggcc atccttctcc tgcgggggct ttttcgcggc ctccgccaga tgccactcct
     1861 tcagctccgg cgcctggagc aaatccgcca gcaaaccggc ataaggcttc ccgcaaggct
     1921 ccagcggggc gcccggattc ccggggacga tccggagcgc gaagagccgg cggcgttccg
     1981 gcttttcctt tcccttcgag tttcgcccat gggaggtgat ccacagcgtc aggtccccga
     2041 tccgggtcgc gccttcgatg tcgcactccg aatatgcctg gtcttcctca tcccatttgg
     2101 cgtcgagaaa ttcatccaat gaaagcgttg ccaccgcctg tccgttgccg tccgcttcaa
     2161 atacccgcac ggtattccgt tcatccgtgc ccgccgcgaa gcgtttcccc cctatatcca
     2221 ccacgcccga cgcgtcgcac gcgccggaga agatcgaggc ctcgcccgag gcaaaccgca
     2281 tcggcgcgga ttcggatagg ggcggcgccg ccgaagccgg cgacgggaag tccggcgcgg
     2341 agacctgggc gtgggccgcg gcggccgcgg gggtggtggc agcgaggatc gggttcgttt
     2401 tcatgatttc gcgggaaagg aagttgcgac ctaaacgaga acgggaccga gctgcgaacg
     2461 atcggcttcc gcctcgactt gctggagcat ctcgtcggcc tccggacgaa cctcggcttc
     2521 ccgcttgtgg tccttgatcg gcgcccaatg gttgcggcgg tggcgcatcg ccagcgcggc
     2581 aaactccgcg gcaaaggccg ggcactgctt ggaaagacgc tggaattcct tgccgtcccc
     2641 ggatccatag tttgcggcgt ccgtcgcggg gcatgacacg ccttgggaaa agacctcgag
     2701 aaagccggag ggattcgccc ggtagtgcgc aaagatcccc ggaagcagcg ggctggccgt
     2761 gcgcgcattg tagctggtct ggaaaaggcc cgcttcggcg gtttccccgg tggtattgcc
     2821 gtccggcgcg taaaggccca tgcagtgttt gccggagctc tcgcgcatgc ccaaacccat
     2881 gagcaggaca aagagatgcc gcagcgtatc aacgccggaa acggagttat ccatgccgag
     2941 agcggcaaat tgcgcgcggt aatgcgccag gacatcgcgc gcggcggtgc tggcggccgc
     3001 cttggccatt tcgacggcgg cgggatcgcc cgccttgaac ttgcaataga cccggccata
     3061 aacgagcgcc atgcccttga tgtaggcgag cggcgcccgc ccgcgctcgt tccatcggta
     3121 acgcgcgatt tccgagccgg ccgcgatctg gagaatcgga tcgaggcttc ccgccggtcc
     3181 gtccggttcg ggtcgaggcg cggaaccgtc gattcgcgac aaagccgcat ccaatgccag
     3241 cagcatctcc ctgcccacct tcccatcggc ctcgatttcc accgactgct ggaaccggat
     3301 cacggccgct tcggtctttg gtccgaaggt tccccggttc cctcccgcct ggatgcggtc
     3361 ctccggcggc agcacccggt cgagggcatc ctgaatgacc cccagcgcct cgccgtggtc
     3421 gcgcccgcgg acgaacgggc ggcgtttgcc gcccgccgcg agttgcagct ccgcgttgcc
     3481 cgccagcggg ggatgcgtca atgccggcac tcccggctcc gcgccaccgc cgcccgcgcc
     3541 gatttcgaga taatcgtcct gaggctgatg ctcgccgcca ttgccggaag ccatgcgcgc
     3601 gtggatccgg gcgatgaggt ccgccttgtt gacgtttcgc cccgggcaat gcgtgtgcgt
     3661 ggtgttgggg tcctccttgt ggaagcgcaa ggtgtccggg ctgatgccgc gccagaggtg
     3721 gagggtcgcc aacgcatcca cggcattttc ccgcacgccc ggattaaacg gctcgtcgtc
     3781 atattccccc accagttcaa cgccccacgc gatgttattc caggaaggcg aatgcacgcc
     3841 gctcgtggtc aggggcgtga agacccagat caggtcgtcg gcgatgaaga ggtgcgggcc
     3901 ggccggccag atcatgcgga agctggggct gcacgacatc gcccggctga cgcgttacgc
     3961 gatccggtgc ggcctggtcg gcacggagga gccgccgccc gcgccccgcg aggcggcccc
     4021 tccggatcgc tgaatcggcc gagccttttc ggttcaatcg gacgcatttc cccggcgggg
     4081 ttggcaaccc cgccccaccg cgaaccggtc tcttgggggg gcggcgttgc caacgccgcc
     4141 gctcggcgcg gcaaacggaa tcgaatgggt tccaacggcc c
//
LOCUS       DATDAY010000237         3448 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_158966, whole genome shotgun sequence.
ACCESSION   DATDAY010000237 DATDAY010000000
VERSION     DATDAY010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 3448)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3448)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3448
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_158966"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(72..2072)
                     /locus_tag="VIS74_04710"
     CDS             complement(72..2072)
                     /locus_tag="VIS74_04710"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEY5792575.1"
                     /translation="MSSFRFFDLNPEQHRAATHPGGPMLILAGAGTGKTRVITARVTW
                     LIANGIPADKILAVTFTNKAARELKERIAGMVEPEKAKLVTASTFHSLCLKILRRDIE
                     RLGYKPNFTIYDEGDQTGLIKKIINRTAAKDESLDPNAAKAGISKAKNMGWSPPEDDK
                     TLLGAVYARYQRELKLVNAIDFDDMLTLSVRLLADFPAVRDHWREKYDHLLVDEFQDT
                     NKLQLDLVTLLCRERRPNICVVGDDDQSIYGWRGAEISNILEFERHFPNPEIFKLEQN
                     YRSTNAILGAANRLIKNNARRRGKKLWSDSKEGANVTVISADHDKTEAEFVVNEINAH
                     KHSERQEWEHFAVLYRMNAQSRLIEGELRRMKIPYRLVGGRSFFDRREVKDVISYLNF
                     LANPRDDASLLRILTAPPRGIGGVTIESAIEFSAKANANLLDSMTSSAFGETLSNRAR
                     NAVQTFADEMQAARIRLMTPGANAAELIGSILADCGYLEDLKRSCKTPEEALSRETNV
                     RELLDALAEYQTKHKDWQGFLDDIQLDRDREKDKEDGRGVTLITLHAAKGLEFPHVFL
                     IGAEEGLLPHERSKLEGTVDEERRLFYVGITRARKTLTISHCLYRTKFGSRITCKSSS
                     FLKEIEGENVDFVSHAEVMARPVKEEEALDLFARAREMINES"
     gene            complement(2126..2524)
                     /locus_tag="VIS74_04715"
     CDS             complement(2126..2524)
                     /locus_tag="VIS74_04715"
                     /inference="COORDINATES: protein motif:HMM:TIGR04256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HEY5792576.1"
                     /translation="MGDETKSKLLCDQIRQIAFDIHVYLGHGHLEKVYENALRHRLQK
                     AGHDVQAQYPIEVFDEDGALIGSYFSDLLVEGCILIEIKTAKNLSPEHEAQILGYLKS
                     TGLEHGLLINFGSYKFEIRKFAKKHPPARE"
     gene            complement(2553..3158)
                     /gene="purN"
                     /locus_tag="VIS74_04720"
     CDS             complement(2553..3158)
                     /gene="purN"
                     /locus_tag="VIS74_04720"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEY5792577.1"
                     /translation="MIRTRMENKIRLGILGSGKGSNFAAILREARAGKLAVEIAAVAS
                     DMPGAGILQLAQEAGLPHAVFPESKFRTKLTPEVEQALADYLKKAGADWLILAGYMRV
                     LKAPLLDAFPGKIINIHPSLLPKFRGLAAWKQALEAGETETGCTVHFVDAGVDTGKII
                     AQVRVPILSGDTAETVHARIQAEEHRLYPEVIARIVSGDLN"
     gene            3214..>3448
                     /locus_tag="VIS74_04725"
     CDS             3214..>3448
                     /locus_tag="VIS74_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEY5792578.1"
                     /translation="MNSDDLTTYLNESSREVDRALNRLLPAAAVKPATLHRAMRYSLF
                     AGGKRMRPILTLAAAEACGGTREAALPAAGAVEC"
BASE COUNT          619 a         1057 c         1028 g          744 t
ORIGIN      
        1 tcccggccgg ccccctagca cccgccttat ctttgtccgg ccgggatcaa cgatcccggc
       61 tacagcccgc ttcagctctc gttgatcatc tcccgcgcgc gggcgaagag atcgagcgcc
      121 tcctcctcct tgaccggccg cgccatgact tccgcgtggc tgacaaaatc cacgttctcg
      181 ccctcgatct ccttgagaaa gctgctcgat ttgcaggtga tccggctgcc gaatttggtg
      241 cgatacaggc aatgggaaat ggtcagcgtc ttccgcgccc gcgtgatgcc gacataaaac
      301 agccgccgct cctcgtccac cgtgccctcg agcttcgagc gctcgtgcgg cagcagacct
      361 tcttccgcgc cgatcaaaaa gacgtgcgga aactccagcc ccttcgccgc gtgcagcgtg
      421 atcagggtca cgccccggcc gtcctccttg tccttttcgc ggtcgcggtc gagctggatg
      481 tcgtcgagga aaccctgcca gtccttatgc ttggtttggt attccgccag cgcgtcgagc
      541 aattcgcgca cgttcgtctc gcggctcagc gcctcctccg gcgtcttgca actccgcttc
      601 aggtcctcca aataaccgca gtcggcgaga atggagccga tcaactccgc ggcgttcgcg
      661 cccggcgtca tcaggcggat gcgcgcggcc tgcatttcat cggcgaaggt ctgaaccgcg
      721 ttgcgcgcgc ggttggaaag cgtctcgccg aaggccgagg aggtcatcga atccagcagg
      781 ttcgcgttgg ccttcgcgct gaattcaatc gcgctttcaa tcgtcacgcc gccgatgccg
      841 cgcggcggcg cggtcaggat gcgcagcagc gaggcgtcgt cgcgcgggtt ggccaggaag
      901 ttaaggtacg aaatgacgtc cttcacctcc cgccggtcga agaagctgcg gccgccgacg
      961 aggcggtagg gaatcttcat gcggcgaagc tcgccttcga tgagccgcga ctgcgcgttc
     1021 atgcgataca gcaccgcgaa atgctcccac tcctgccgct cggaatgttt gtgcgcgttg
     1081 atctcgttca cgacaaactc cgcctccgtc ttgtcatggt cggcggagat gaccgtgacg
     1141 ttcgcgcctt ccttggagtc gctccagagc tttttgccgc ggcggcgcgc gttgtttttg
     1201 atcagccggt tggccgcgcc gagaatggcg ttcgtgctgc ggtagttctg ctccagcttg
     1261 aaaatttccg gattcggaaa atggcgctcg aattcgagga tgttggaaat ctccgcgccg
     1321 cgccacccgt agatgctctg gtcatcgtcg ccgaccacgc agatgttcgg ccgccgttcg
     1381 cgacagagca gggtgaccag gtcgagctgg agtttgttcg tatcctgaaa ttcgtccacg
     1441 aggagatggt cgtatttctc ccgccaatgg tcgcgcacgg ccggaaaatc ggccagcagc
     1501 cggacgctga gcgtcagcat gtcgtcgaaa tcgatcgcgt tgacgagctt gagctcgcgt
     1561 tgatagcggg catacaccgc gccgagcagc gtcttgtcat cctcgggcgg agaccatccc
     1621 atgttcttcg ccttgctgat gccggccttc gcggcattgg gatcaagcga ctcgtccttc
     1681 gcggcggtgc gattgatgat ttttttgatc aggccggtct ggtcgccctc gtcgtaaatg
     1741 gtgaagttcg gtttgtagcc taaccgctca atgtcgcggc ggaggatttt caagcagagc
     1801 gaatggaacg tcgaagccgt gacgagcttc gccttttccg gttcgaccat gcccgcgatg
     1861 cgctccttga gttcgcgggc ggccttgttc gtgaaagtga cggccagaat cttgtccgcg
     1921 ggaatgccat tggcaatcag ccaggtcacc cgcgcggtga tcacgcgcgt cttgcccgtg
     1981 cccgcgccgg cgagaatgag catcggcccc ccgggatgcg tggccgcgcg atgctgctcg
     2041 ggattaaggt cgaaaaagcg gaaggaactc atgggaaatt taaccgtaga gaacgcaaag
     2101 agcgcagaga aaatggaaat gcattctatt cacgagcggg cggatgtttt ttggcgaatt
     2161 ttcggatttc aaatttgtaa gagccgaagt tgatcagcag gccgtgttca aggccggtgg
     2221 attttaagta tccgagaatc tgggcctcat gctcagggga caggtttttt gcggttttaa
     2281 tttcaatcag aatgcagcct tcgacgagca gatcggaaaa atagctgccg atcaaggcac
     2341 cgtcttcatc gaatacctcg atgggatact gggcttgaac gtcatgtccg gctttttgca
     2401 ggcgatgccg caacgcattc tcataaactt tttccagatg tccgtggccg agataaacgt
     2461 gaatatcgaa cgcaatctgc cgaatttgat cgcagagcaa tttcgacttc gtttcgtccc
     2521 ccatttcttt gtgttctctg cgttctttgc ggttaattca aatccccgct gacgattcgc
     2581 gcgatgactt ccggatacag ccggtgttcc tcggcttgga tccgcgcgtg cacggtttcc
     2641 gcggtgtcgc cggaaaggat cggcacgcgg acctgggcga tgattttgcc ggtatccact
     2701 cccgcgtcca cgaagtgcac cgtgcagccg gtttcagtct ccccggcctc cagcgcctgt
     2761 ttccaggcag ccaggccgcg gaatttcggc agcagcgagg gatgaatgtt gatgattttt
     2821 cccgggaagg cgtccagcag cggcgccttg agcacccgca tgtaaccggc gagaatcagc
     2881 cagtccgctc cggccttttt caaatagtcc gccagagcct gctccacttc cggcgtcaat
     2941 ttggtccgga acttcgattc cgggaaaacc gcatggggga gtcccgcctc ctgggcgagc
     3001 tggagaatgc ccgcgccggg catgtccgag gccacggcgg cgatttcgac cgccaatttc
     3061 cccgcccgcg cctcgcgcaa aatcgccgca aaattcgacc cctttccgga accgagaatg
     3121 ccgagccgta ttttgttttc catgcgggtg cggatcatgc acaaagtcgc tttcactggc
     3181 cagcgaaacg cggcataact gcatccgatt tttgtgaatt ccgacgactt aacgacttac
     3241 ctgaacgagt cctcccgcga agtggaccgg gcgctcaacc gcctgcttcc cgccgccgcg
     3301 gtcaagcccg ccacgctgca ccgggccatg cgctacagcc tgttcgccgg cggcaaacgc
     3361 atgcggccga tcctcacgct ggcggcggcg gaagcctgcg ggggaacccg cgaggcggcg
     3421 cttccggcgg ctggcgcggt ggagtgcg
//
LOCUS       DATDAY010000238         6607 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_2119423, whole genome shotgun sequence.
ACCESSION   DATDAY010000238 DATDAY010000000
VERSION     DATDAY010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 6607)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6607)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6607
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2119423"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..892
                     /locus_tag="VIS74_04730"
     CDS             <1..892
                     /locus_tag="VIS74_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006983067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY5792579.1"
                     /translation="WFDQDDVTNLPPHKRNTGMMFQSYALWPHLTVAKNVAFGLEERR
                     VPKAEIETRVREALQSVRMAPYAGRKIAQLSGGQQQRVALARALVIRPRCLLLDEPLS
                     NLDAKLRLEMRTEIRRVCKQFGLTAIYVTHDQKEALSVSDRMAILEGGRIAQIGTPQE
                     VYRRPRSRIVADFIGETNFIEGTLLGMANGFAQVETSIGRFQGVLSDPERPPAPGAKV
                     TLSIRPEAFSIQTAPQAVNSVRGRIGELVYLGEVAQYHFNASGHSLKIFELNPHLEAR
                     PRDIDLYAAAAPNDVMVLEG"
     gene            984..2576
                     /locus_tag="VIS74_04735"
     CDS             984..2576
                     /locus_tag="VIS74_04735"
                     /inference="COORDINATES: protein motif:HMM:NF024739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY5792580.1"
                     /translation="MFRIAAVFAALAIVVGIPFALKPKQNLLAAADDTLVIISPHNGA
                     IRYEFTRAFAEFYQKQTGRTVRIDWRLVGGTSEIAKYLKSEYYAAFQRAWVAEGRAWN
                     AEVEAAFDNPQVKPSESSGAGTPAQAARRAFLKSDAGIGIDLFFGGGSYDFIQQANAG
                     RLVDSGLLRAKPEWFTDAAIPPSVSGETYYDRAGRWIGACISSFGICYNTDSLRRLGF
                     TSIPAAWSDLADPRFVQQVALADPTKSGSAAKAYEMIIQQQMQERVAKLGETPEALAA
                     GWAAGLRIIQRVSANARYFTDAASKVPLDVSAGDAAIGMCIDFYGRFQSEAVKLADGS
                     SRLQYFTPAGGSSPSVDPIGQLRGAPNAKIARFFMEYALSLEGQKLWNFRVGAPGGPV
                     RYALRRLPVLKELYAPQYAEYRSDPEVNPYREAGTFYYHSEWTGHLLRVLSFIIRNLS
                     LDSHDELTAAWQALIAADFPPEATAKFSDLSAVDYALAQGRLRDVTRSADRIQEVQLA
                     QELGDHFRNQYREAARLAKEGR"
     gene            2674..4368
                     /locus_tag="VIS74_04740"
     CDS             2674..4368
                     /locus_tag="VIS74_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006983262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEY5792581.1"
                     /translation="MSKPLSIFVFALTSVFFFFFFAWPIGETLRGAFVNPDGSFTAAY
                     LIEVFRNPIYLEGLWNSLLMAVFSTALAILIALPLAFISDRFLFPGKSLLSALVLIPM
                     ILPPFVGAIGVKQILGQEGALNALLAAIGWMDAAHPIDWLGQGRLGGVILMNALHLYP
                     ILYLNLVAALANIDPAMEEAAENLGCTGFRKFRKITLPLVMPGLFAGGTIVFIWAFTE
                     LGVPLMFEFTRVTSVQIFYGIKDIGGNPFPFALVAVMLVSTVLLYAASKYAFGRNSHA
                     MMAKATSQGGPRPAGPLVSIFCTAFFAGVTFLAVLPHLGVIFVAFSTDWYQTILPTGW
                     TLEHFREALGHNLTLPSIQNSLKYAGLATLLDVVLGIAIAYVVTRTKLAGREILDAMA
                     MLPLAVPGLVLAFGYLAMTQEGKIFGFMNPAVDPLLILVIAYSVRRLPYVVRSAAAGF
                     QQTSVTLEEAAQNLGCPPFRALRKITLPLIAANLLAGGLLAFSFAMLEVSDSLILAQK
                     QQDFPITKAIYELYQLLGDGRYIASALGVWAMVFLGVTILGASLMLGKKLGAIFRA"
     gene            4383..4631
                     /locus_tag="VIS74_04745"
     CDS             4383..4631
                     /locus_tag="VIS74_04745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792582.1"
                     /translation="MKIILAILLAGAAGFIHAADDSVKPYPLTVCAVSGAKLGTMGEP
                     VVRVYDGQEVKFCCGGCIAMFEADPAKYLKQVRAAENK"
     gene            4686..5291
                     /locus_tag="VIS74_04750"
     CDS             4686..5291
                     /locus_tag="VIS74_04750"
                     /inference="COORDINATES: protein motif:HMM:NF028235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEY5792583.1"
                     /translation="MRPESETKLKLLRTAIELIAESSYGSVSVDDICQRAGVKKGSFY
                     HFFPSKSDLTVAAMEADWQAGQIRLDRIFSSQTPPLERLEAYFESSREIQALKQAQCG
                     KICGCPMITLGAELSTQDEKIRLKTVEIVNRYLKYFESAVRDAAEEGTIETGNARLLA
                     EELFAYFQGSMLQAKIHNDLKPLLALKKGLSRFLEPRPATV"
     gene            5334..6410
                     /locus_tag="VIS74_04755"
     CDS             5334..6410
                     /locus_tag="VIS74_04755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkene reductase"
                     /protein_id="HEY5792584.1"
                     /translation="MKLPDLLDPVRIGDWHLPNRIVMAPLTRARAGKERIPNALMAEY
                     YVQRASAGLILSEATSVTPRGVGYAETPGIWSAEQVEGWKLITKAVHEKGGRILLQLW
                     HVGRISDPMFLDGQLPVAPSAVRPAGHVSLVRPMKPFVTPRALAREEIPGIVEAYRRG
                     AENALAAGFDGVEIHGANGYLLDQFLQDRTNHRTDEYGGSIENRARLMLEATDAAISV
                     WGASRVGMHLSPRGDIHDMGDSNPAVTFGYVAQELGRRGIAFLCAREHPGDDRLGPKL
                     KKIFGGAFIANEKFTRVTANQVLAAGEADAVAFGVPFIANPDLVERFVRDEALNEADH
                     DTFYAPGSKGYTDYPILEPAALTA"
     gene            6410..>6607
                     /locus_tag="VIS74_04760"
     CDS             6410..>6607
                     /locus_tag="VIS74_04760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HEY5792585.1"
                     /translation="MKAIGYQKSLPISDPESLLDLEMPEPLATGRDLLVRVKAVSVNP
                     VDAKQRLRAQPAPGEANILGYD"
BASE COUNT         1202 a         2025 c         2047 g         1333 t
ORIGIN      
        1 ttggttcgat caggacgacg tgaccaatct cccgccgcac aagcggaaca cgggcatgat
       61 gtttcagagc tacgcgctct ggccgcacct gacggtggcc aaaaacgtgg cgttcggcct
      121 ggaggaacgc cgggtgccga aggcggaaat cgaaacgcgc gtccgggagg cgctccagtc
      181 cgtccgcatg gctccgtatg ccgggcggaa gatcgcccag ctctccggcg gccagcagca
      241 gcgcgtggcc ctcgcgcgcg cgctggtgat ccgcccgcgc tgcctgcttc tcgacgagcc
      301 gctttccaat ctcgacgcca agctccgcct cgaaatgcgc acggaaatcc gccgcgtctg
      361 caagcaattc ggcctgacgg cgatttacgt gacgcatgac caaaaggagg cgctgtccgt
      421 ctccgaccgc atggcgattc tcgaaggcgg ccgcatcgcg cagatcggca cgccgcagga
      481 ggtgtatcgc cggccgcgtt cccggatcgt ggcggatttc attggcgaaa cgaatttcat
      541 cgaaggcacg ctgctgggga tggcgaatgg gttcgcgcag gtcgaaactt cgatcggccg
      601 ttttcagggc gtgcttagcg atccggaacg gccgcccgcc cccggagcga aagtcacgct
      661 gtccattcgc cctgaggcct tctccattca aaccgccccg caggccgtga actccgtgcg
      721 cggccggatc ggcgagctcg tttatctcgg cgaggtggcg caataccact tcaacgcgag
      781 cgggcattcg ctgaaaattt tcgaactcaa cccgcacctg gaagcgcggc cgcgggacat
      841 tgatttatac gcggccgccg cgccgaacga cgtcatggtg ctggaggggt agttttaagt
      901 ttgtaagtct taagttttaa gtgaaaacgc aacgctccaa gtccggttta agacttacaa
      961 acttaaaact taaaacttaa gatatgttcc gcatcgccgc cgtttttgcc gctctggcca
     1021 tcgtggtggg aattcccttc gcgctgaagc cgaagcagaa cctgctggcc gcggcggacg
     1081 acacgctggt gatcatcagc ccgcacaacg gggcgatccg ctacgagttc acgcgggcgt
     1141 tcgccgaatt ttaccaaaag caaaccgggc gcaccgtgcg gatcgactgg cggctggtcg
     1201 gcggcacgag cgagattgcg aaatatttga agtcggaata ttacgccgcc ttccagcgcg
     1261 cctgggttgc cgaaggccgg gcctggaacg cggaggtgga ggccgcgttt gacaacccgc
     1321 aggtcaaacc ctccgaatcg tccggggccg gcacgcccgc gcaggccgcc cggcgggcgt
     1381 ttttgaagtc ggacgccggc atcggcattg atttgttttt cggcggcggc tcctacgatt
     1441 ttattcaaca ggccaatgcc ggccggctgg tggattccgg attgctgcgg gcgaagccgg
     1501 agtggttcac ggatgcggcc atccccccga gcgtgtccgg cgagacgtat tacgaccggg
     1561 cgggccgctg gatcggcgca tgcatttcga gtttcggcat ctgctacaac acggattcgc
     1621 tccggcgcct gggcttcacg tcgatccccg ccgcgtggtc cgacctcgcc gatcctcgct
     1681 tcgttcagca ggtggccctg gccgatccga ccaagagcgg ttccgccgcg aaggcctacg
     1741 aaatgatcat ccagcagcaa atgcaggagc gggtggcgaa gctcggcgag acccccgagg
     1801 cgctggcggc ggggtgggcc gcggggctgc ggatcatcca gcgcgtgtcg gcgaacgcgc
     1861 gttatttcac cgacgcggcg agcaaggtgc cgctcgacgt ttccgccggg gatgcggcca
     1921 tcggcatgtg cattgatttt tatgggcgct tccagagcga ggccgtgaag ctggccgacg
     1981 gttcctcgcg gctgcaatat ttcacgccgg ccggcggctc ctccccgagc gtggatccca
     2041 tcggccagct gcgcggcgcg ccgaatgcga aaattgcccg gtttttcatg gaatacgcgc
     2101 tttcgctgga ggggcagaaa ttgtggaatt ttcgcgtggg cgcccccggc ggcccggtgc
     2161 gttacgcgct gcgccggctg ccggttctca aggagctcta cgcgccgcaa tacgcggaat
     2221 accgttccga ccccgaggtg aatccttacc gggaggcggg cacgttttat tatcacagcg
     2281 aatggaccgg ccatctgctt cgggtgctga gcttcatcat tcgcaacctg agtctggact
     2341 cgcacgacga actgacggcg gcctggcagg cgttgatcgc ggcggatttc ccgccggagg
     2401 ccacggcaaa attttccgac ctgagcgcgg tggattacgc gctggcgcaa ggccggctcc
     2461 gcgatgtcac gcgttccgcg gaccgcatcc aggaagtcca gctcgcccag gaactcggcg
     2521 accattttcg taatcaatac cgcgaagccg cccggctggc gaaggagggg aggtgaggag
     2581 ttttaagttt gccgttttaa gttttaagca aagtgccgct gcgaacgttc gttttcttac
     2641 aaacttaaga cttaaaactt aaaactgggc cgcatgagca aaccactttc catcttcgtc
     2701 ttcgccctca cctcggtgtt tttctttttc ttcttcgcgt ggccgatcgg cgagacgctg
     2761 cgcggggcgt ttgtgaatcc cgacggcagc tttaccgccg cttatttgat cgaggttttt
     2821 cgcaatccca tctacctcga aggtctctgg aattcgctgc tcatggcggt tttcagcacg
     2881 gcgcttgcca tcctgatcgc gctgccgctg gcgttcatct cggatcgttt tctgttcccg
     2941 ggcaagtcgc tcctcagcgc cctggtgctc attccgatga tcctgccgcc cttcgttggc
     3001 gcgatcggcg tgaagcagat cctcggtcag gagggcgcgt taaacgcctt gctggcagcc
     3061 atcgggtgga tggatgccgc gcatcccatt gactggctcg gccaggggcg gctggggggc
     3121 gtcatcctca tgaacgcgct gcacctgtat ccgatcctct atctgaacct cgtcgcggcg
     3181 ctggcgaaca tcgatcccgc catggaggag gccgcggaaa atctgggctg caccggcttt
     3241 cgcaagttcc gcaaaatcac gctgccgctc gtcatgcccg gcctgtttgc cggcggcacc
     3301 attgttttca tctgggcgtt caccgaactc ggcgtgccgt tgatgtttga atttacccgc
     3361 gtcacttcgg tgcagatttt ttacggcatc aaggacatcg gcggaaaccc cttcccgttc
     3421 gcgctggtcg cggtgatgct ggtttcaacg gtgctgcttt acgcggccag caaatacgcc
     3481 ttcggccgga acagccacgc gatgatggcc aaggcgacca gccagggagg gccgaggccg
     3541 gccggtccgc tcgtttcgat tttttgcacc gcgtttttcg ccggggtgac gttcctggcg
     3601 gtgctgccgc acctcggcgt gatctttgtg gcgttttcga ccgactggta tcaaacgatc
     3661 ctgcccaccg gctggacgct cgagcatttt cgcgaggcgc tgggccacaa tctgacgctg
     3721 ccttcgatcc aaaacagcct caaatacgcg ggcctcgcga cgctgctcga cgtggtgctc
     3781 ggcatcgcca tcgcttacgt ggtgacccga acgaagctcg cggggcgcga gattctcgat
     3841 gccatggcca tgctgccgct ggcggtgccc ggcctcgtgc tggcgttcgg ttatctggcc
     3901 atgacgcagg aaggcaaaat tttcgggttc atgaatccgg cggtggatcc cctgctgatt
     3961 ctcgtgatcg cctacagcgt gcgccggctg ccctacgtgg tccgctccgc cgccgcgggc
     4021 ttccagcaaa cgagcgtcac gctggaggaa gccgcgcaaa acctcggctg cccgcccttc
     4081 agggcattgc gcaaaatcac cctgccgctc atcgccgcca atctcctggc gggcggcctg
     4141 ctggcgtttt cgtttgcgat gctcgaagtc agcgactcgc tcatcctcgc gcagaagcag
     4201 caggactttc ccatcaccaa agccatctac gaattgtatc aactcctcgg cgacggccgt
     4261 tacatcgcca gcgcccttgg cgtgtgggcg atggtttttc tcggcgtgac gatcctcggc
     4321 gcgagtttga tgctgggcaa aaaactgggt gcgatcttcc gggcttaagt taacttccgg
     4381 ttatgaaaat cattctcgca atcctcttgg ccggggccgc cggattcatc cacgcggcgg
     4441 acgacagcgt caaaccttat ccgctgacgg tttgcgcggt ctcgggagcg aaactgggaa
     4501 cgatgggcga gccggtcgtc cgggtttacg acgggcagga agtcaagttc tgctgcggcg
     4561 gctgcatcgc gatgttcgaa gccgaccccg ccaaatacct gaaacaagtg cgggcggcgg
     4621 aaaataaata agccaaaaca aaaaatcgct tgcgcttgac tgattggtca aatagggtgc
     4681 ccggcatgag gcccgagtcg gaaaccaaac tcaagctgtt gcgcacggcg atcgaattga
     4741 ttgccgaaag cagctatggt tccgtgagcg tggatgacat ctgccagcgc gccggggtca
     4801 agaagggcag cttttatcat tttttcccct ccaaatccga cctgaccgtg gcggcgatgg
     4861 aggccgactg gcaggcgggg caaatccggc ttgaccgcat tttttcctcg cagaccccgc
     4921 cgctggagcg gctcgaagct tattttgaat ccagccgcga aatccaggcc ctcaagcagg
     4981 cgcaatgtgg gaaaatctgc ggctgtccga tgattacgct gggcgcggag ctgagcacgc
     5041 aggatgaaaa aatccgcctg aaaacggtgg aaatcgtcaa tcgctacctc aaatactttg
     5101 aatccgccgt gcgggatgcc gcggaggagg ggacaatcga aacggggaac gcccggctgc
     5161 tcgcggagga gttgttcgcc tattttcagg gaagcatgct gcaagcgaag attcacaatg
     5221 acctgaagcc gctgctggcc ttgaaaaaag ggctttcccg atttttggaa ccccgtcccg
     5281 ccaccgtctg agtttttttt gccctaactc tttaccaacc agtcaactta tttatgaaat
     5341 tacctgattt gctcgacccg gttcggatcg gcgattggca tttgcccaat cgaatcgtca
     5401 tggcgccctt gacccgggcc cgcgccggga aggaacgcat tccgaatgcc ttgatggccg
     5461 aatattacgt ccagcgcgct tcggcggggc tgatcctttc cgaggccacg tcggtgactc
     5521 cgcggggcgt cggttatgcc gagacgccgg gaatctggtc cgccgagcag gtggagggct
     5581 ggaagctcat cacgaaggcg gtgcatgaaa agggcggccg gattctgctg cagctctggc
     5641 acgtgggccg gatttccgat ccgatgtttc tcgacggcca gttgcccgtc gcgccaagcg
     5701 cggtgcggcc ggccgggcac gtgagcctcg ttcgtccgat gaagccattt gtcacgcccc
     5761 gcgcgctggc acgggaggaa attcccggca tcgtcgaggc ctatcgccgg ggagcggaaa
     5821 acgccttggc ggcgggattc gacggcgtgg aaatccacgg cgccaacggc tacctgctcg
     5881 atcaattttt gcaggaccga accaatcacc gcaccgacga atacggcgga tcgatcgaga
     5941 accgggcgcg gttgatgctg gaagccacgg acgccgccat ctccgtctgg ggcgcttccc
     6001 gcgtcggcat gcacctctcg ccgcgcggcg atatccacga tatgggcgat tcgaatcccg
     6061 ccgtgacctt tggttacgtc gcccaggaat tgggccggcg cggcatcgcc ttcctgtgcg
     6121 cccgggaaca tcccggcgac gaccggctcg ggccgaaact gaaaaagatt ttcggcggcg
     6181 ccttcatcgc gaacgaaaaa tttaccaggg tgacggccaa tcaagtgctc gccgcgggcg
     6241 aggcggacgc ggtcgcgttt ggcgtgccgt tcatcgccaa tccggatctg gtggagcgct
     6301 tcgttcggga tgaggcgctc aacgaggccg atcacgacac gttctacgcg ccgggatcga
     6361 agggctacac cgattatccc attctggaac ctgctgccct caccgcctga tgaaagccat
     6421 cggttatcaa aaatccctgc ccatttccga tccggaatcg ctgctggatt tggaaatgcc
     6481 cgaacccctc gcgaccggcc gggatctgct ggtgcgggtc aaggcggtct cggtgaatcc
     6541 cgtggacgcc aagcagcgcc tgcgcgccca gcccgcgccg ggggaggcga acattctcgg
     6601 ttacgat
//
LOCUS       DATDAY010000239         3578 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_2417101, whole genome shotgun sequence.
ACCESSION   DATDAY010000239 DATDAY010000000
VERSION     DATDAY010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 3578)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3578)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3578
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2417101"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1192
                     /locus_tag="VIS74_04765"
     CDS             <1..1192
                     /locus_tag="VIS74_04765"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEY5792586.1"
                     /translation="AGCKPKPPADPVPPKPVAAATPAPAPAAAPAPEPIDKTAQAVVL
                     GYHRFENPPRDPLALSTAEFRQQMQALKDLGIAVIPMDDFLAWRRGEKSIPARSAVIT
                     IDDGYNDSYSQAWPVLKEFGYPFTMYVYTDYISKGGRSITWGQLEQMRDAGVDIGSHT
                     VSHDNLVKPKRAKNANYGEWLMNELKGSKDLIEQHLGIKVKTLAYPFGIHNANVMAQG
                     LAAGYEALFTVEGKKIGFDVDPAKVGRYVIQSGHPIIFKAATSFGSATLAPAAVSAGA
                     PAGAQKGSIPTLPAAGETIGEQMPLIEADLSSMGNVDPKSVEMRISGFGQVSAVYDPV
                     SKKLTYQPVQRLRERVTTIAVSAKGPNGRVETRWSFTIDPATAVPTPEREVPAPAPET
                     LPQS"
     gene            1195..2544
                     /locus_tag="VIS74_04770"
     CDS             1195..2544
                     /locus_tag="VIS74_04770"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEY5792587.1"
                     /translation="MSEAAASSAPKLARHIGLAALVIYGIGDMVGAGIYGTIGVAAGV
                     MGNAVWLAFVLAMLAAMLTGLSYASLASRYPRAAGAAYVTQRAFGFRFLSYLVGLAVA
                     ASGLTSMAAGSRVFAANFQPLIGAVPLWCIFLVFIAVLTVINLRGIRECVAVNIVCTL
                     VEVGGLLFVIAVGLPYLGSVNYLETPPAAGGLSLPLIMGGAVLTFYAFVGFEDLLNLA
                     EEVKNPRKTMPWGIILAVGTVTVVYVLVSIVAVSVVPYARLADPANGAPLAQLTSRAA
                     PWLPPGVFTFITLFAVANTALMNFIMGSRLVYGMSRQGLMPAVLGKVHPTRHTPHVAI
                     LVLAGIVALLAFAGDIKDLASATSILLLLVFGLMNGSLIVLRRRKGEEPGGFEIPVFV
                     PVLGALVCLGLVATRVFGTAGGVEAVRIAGVMLAGIVILYFVVRPKAEAMAAEIPED"
     gene            complement(2541..2978)
                     /locus_tag="VIS74_04775"
     CDS             complement(2541..2978)
                     /locus_tag="VIS74_04775"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF013607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HEY5792588.1"
                     /translation="MKPTVLILCTGNSCRSHLAEGILRAAAGDLLNVQSAGSKPAGYV
                     HPKAMAVMREIGIDLSAHRSKPLAEFLDQSVETVITVCGNADQACPMFPGQVNRHHWG
                     FDDPAHATGTDEEVLNVFRRVRDEIRRTFEAYAAGRRDAAAKN"
     gene            complement(2975..>3578)
                     /locus_tag="VIS74_04780"
     CDS             complement(2975..>3578)
                     /locus_tag="VIS74_04780"
                     /inference="COORDINATES: protein motif:HMM:NF013885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="arsenical-resistance protein"
                     /protein_id="HEY5792589.1"
                     /translation="VAVNDLIMVFAFAPIVMLLAGVSGVKIPAAVMLVSVLVFIVIPL
                     AAGGLSRSWLIARRGTAWFEQQFLPRFRPVTIFALLATLCLIFAFQAENLLRNWFAVL
                     LLAIPILIQVYFNSGLAYGLMRLFKVRHNVAAPGALIGASNFFELAVAVAITLFGPTS
                     PAALATVVGVLVEVPVMLSVCRLCTGSRGWYERPLHPQSS"
BASE COUNT          598 a         1115 c         1219 g          646 t
ORIGIN      
        1 tgccggctgc aagcccaagc cgcccgcgga cccggtgccg cccaagccgg tggcggcggc
       61 cacgcccgcg ccggctccgg ccgccgcgcc ggctccggag ccgattgata aaaccgcgca
      121 ggcggtcgtg ctcggctatc accgttttga aaatccgccg cgtgacccgc tggcgctcag
      181 cacggcggaa ttccgccagc agatgcaggc gttgaaggat ctcggcatcg ccgtcattcc
      241 gatggacgac ttcctcgcct ggcggcgcgg ggagaaaagc attcccgccc gctcggcggt
      301 catcaccatc gacgacggct acaacgacag ctactcccag gcctggccgg tgctgaagga
      361 atttggctat cccttcacga tgtatgtcta taccgactac atcagcaagg gcggccgttc
      421 gatcacctgg ggccagctgg aacaaatgcg cgacgcggga gtggacatcg gcagccatac
      481 ggtttcgcat gacaatctgg tgaagcccaa gcgggccaag aacgccaact acggcgagtg
      541 gctcatgaac gaactcaagg gctcgaagga tctcatcgag cagcacctcg gaatcaaggt
      601 gaagacgctg gcgtatccct tcggcattca caacgccaac gtgatggccc agggcctcgc
      661 ggccggttac gaggcgttgt tcacggtcga gggcaaaaag atcggtttcg atgtcgatcc
      721 ggcgaaggtc ggccgttatg tgatccaatc cggccatccc atcatcttca aggcggccac
      781 ctcctttggt tccgccaccc tggctccggc ggcggtttcc gccggcgcgc cggcgggcgc
      841 gcagaagggt tccattccga ccctgccggc ggcgggcgag accattggcg agcagatgcc
      901 gttgatcgag gccgatcttt cctcgatggg caacgtggac cccaagagcg tggagatgcg
      961 catcagcgga ttcgggcagg tgtcggcggt gtatgatccg gtgagcaaaa aattgacgta
     1021 tcagcccgtg cagcgcctgc gcgaaagggt gacgaccatc gccgtctccg cgaaggggcc
     1081 gaatggccgc gtcgaaacgc gctggagctt taccattgat cccgccacgg ccgtgccgac
     1141 gccggagcgc gaggtgcccg cgccggcccc ggaaaccctg ccccagagct gaccatgtcc
     1201 gaagccgccg catccagcgc tcccaaactc gcccggcaca tcgggttggc ggcgctggtg
     1261 atttacggca tcggcgacat ggtgggagcc gggatttacg gcaccatcgg cgtggcggcg
     1321 ggggtgatgg gcaatgccgt ctggctggcg tttgtgctgg ctatgctcgc ggcgatgctg
     1381 accgggcttt cctacgcttc cctggcttcc cggtatcccc gcgcggcggg cgcggcttac
     1441 gtgacgcagc gcgcgtttgg gttccgcttt ttgtcctacc tggtcggatt ggcggtggcg
     1501 gcttccgggc tgacctccat ggcggcgggc agccgggtgt tcgcggccaa tttccagccg
     1561 ctgatcggcg cggtgccgct ctggtgcatt ttcctggtct tcatcgcggt gctgacggtc
     1621 atcaatctgc gcggcatccg cgagtgcgtc gcggtgaaca ttgtctgcac gctggtcgag
     1681 gtgggcggcc tgctgtttgt gatcgccgtg ggactgccct atctgggatc ggtgaattac
     1741 ctggagacgc cgcccgccgc cggcggcctc tccctgccct tgatcatggg cggagcggtt
     1801 ctgacttttt acgcgttcgt cggtttcgag gatttgctca atctggccga ggaggtcaaa
     1861 aatccccgta aaacgatgcc gtggggaatc atcctcgcgg tcggaacggt gaccgtggtt
     1921 tacgtgctgg tcagcatcgt ggcggtctcc gtcgttccct acgcgcggct ggccgacccg
     1981 gcgaacgggg cgccgctggc ccagctcacg agccgggccg ccccctggct gccgcccggc
     2041 gtttttacct tcatcacgct gttcgccgtc gcgaataccg cgctcatgaa cttcatcatg
     2101 ggctcccggc tcgtttacgg catgtcccgc cagggattga tgccggcggt cctgggcaag
     2161 gtgcatccca cgcggcacac gccgcatgtg gccattctcg tgctggccgg gatcgtggcg
     2221 ctgctggcct tcgccggcga catcaaggat ctcgcctcgg ccacgagcat tctgctgctg
     2281 ctggttttcg ggttgatgaa tgggtcgctg atcgtgctgc ggaggcgaaa aggcgaggaa
     2341 cccggcggct ttgaaatccc ggtgtttgtg cccgtcctgg gagcactggt gtgcctggga
     2401 ttggtcgcca cccgggtttt cggcacggcg ggaggggtcg aggcggtccg gatcgccggg
     2461 gtcatgctgg ccggaatcgt gatcctttat ttcgtcgtcc gccccaaggc ggaggcgatg
     2521 gccgcggaaa ttccggagga ttaatttttc gccgcggcat cccgtcgtcc ggccgcgtag
     2581 gcttcgaacg tccgccgaat ctcatcccgc acgcggcgga agacattcaa gacttcctcg
     2641 tccgttcccg tggcgtgcgc gggatcgtcg aagccccagt gatgtcggtt cacctggccg
     2701 gggaacatcg ggcaggcctg gtcggcgttg ccgcaaacgg tgatgacggt ctccacggat
     2761 tgatcgagga attcggcgag cggcttggag cggtgcgccg aaagatcgat gccgatttcc
     2821 cgcatgacgg ccatggcttt cgggtgcacg tagccggccg gcttggaacc ggcgctttgg
     2881 acattgagca aatctccggc ggcggcgcgc aggatgcctt cggcgaggtg gctgcggcag
     2941 gaattgccgg tgcagaggat caggacggtg ggtttcatga ggattgcggg tgaagggggc
     3001 gctcatacca gccgcggctg cccgtgcaga ggcggcagac ggacagcatg acgggcactt
     3061 ccacgaggac gcccaccacg gtggccagcg cggcggggct ggtcgggccg aagagggtga
     3121 tggccaccgc cacggcgagc tcgaagaaat tgctcgcgcc gatgagcgcg ccgggcgcgg
     3181 cgacgttgtg gcggaccttg aacagccgca tgaggccgta ggccaggccg gaattaaaat
     3241 acacctggat caaaatggga atcgccagca gcaggacggc gaaccagttc cgcagcaggt
     3301 tttccgcctg gaaggcgaag atcaggcaca gcgtggccag cagcgcgaaa atggtgaccg
     3361 ggcggaaccg cggcaggaat tgctgttcaa accacgccgt cccgcgccgc gcgatcagcc
     3421 acgaccggct gagcccgccg gccgcgagcg ggatgacgat gaataccagg accgaaacga
     3481 gcatgaccgc ggccggaatt ttcaccccgc tgactcccgc cagcagcatg acgatcggcg
     3541 cgaaggcgaa gaccatgatg aggtcgttga cggccacc
//
LOCUS       DATDAY010000240         2876 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_742031, whole genome shotgun sequence.
ACCESSION   DATDAY010000240 DATDAY010000000
VERSION     DATDAY010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2876)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2876)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2876
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_742031"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(120..2231)
                     /locus_tag="VIS74_04785"
     CDS             complement(120..2231)
                     /locus_tag="VIS74_04785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792590.1"
                     /translation="MEINELARTLLSSSQSIYFKENARISSFLQAHPNSGQGHLQATF
                     LLGTIALNDYSGDFRDIRIPLNRMIAHLAAADALGVPRDHPNRQFVEVLRLTLCGQQA
                     DALAALKTWKPPQGFEAILADWQEIFRLRNTQDWRESRSAALHGSPALKREYFRALVK
                     AINATAGLDFLREAKIQPDPALWRIANESSLSVSDGHTFSKPILEVEFEETAEAAREF
                     GIKPSKDDLSWLKDYLDAPEGSPVLMENGKPVIQVAGKNLFAGYSQRNLMQGLNSLFT
                     FLNDQWGVTDQASGLEEFIKQKLPELKYKPFLIRMIARDNPARISANAVCTALIHDQP
                     SMVTPALWSSLRTDQDGNDLHLSFPDFHSWFHPEVPYGTAFESGSRLYKIGVGDENND
                     AWMKTLWERAPYSYPIALQNAYLENDRSFANLSAEILGKWAGPLADYNLQVMRRLADC
                     DEGQPALYAEAMEKAAKLDPNCYLILGNYLVGQDLPEKAAEAYLKAFEQATDRVWMAN
                     RSKWLVKYLFEKGGVEMANKVAADAADVYSYNGLEAFIWLKEREGKWEEALETARKID
                     KRYEDDQPVNESACLFRMYEVLPDMAIALGYEDKVKKVFPEGARKVLLKHFSSAPIRG
                     VLISGSSQAMVPFGLRKGYVIVALNGYQTDTFDQYTAIRLLSDDPRMSLIVWDGSKYR
                     VCEGDLPSRRFRVEMKDFAAK"
     gene            complement(2283..2621)
                     /locus_tag="VIS74_04790"
     CDS             complement(2283..2621)
                     /locus_tag="VIS74_04790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792591.1"
                     /translation="MKIPTLVAVPIIAFAVLIPLGIGYLQFRGQNTQAEPPAVVNVSL
                     PYQTEEGWTVTQVAQFLSDYGALANGKKMRPLSAQPASGADASETYQIQWDGKTTAIS
                     LAAGVWNPAS"
BASE COUNT          689 a          815 c          736 g          636 t
ORIGIN      
        1 ggggagtaaa actgggtgag atcgcagaat ttcacgaaga cgaaggaagg gagagaatct
       61 gccctgcaga tgaaccgtca gagaagaatt cgtcaactct ccgaattcat ttccctcgct
      121 tatttcgcgg caaaatcctt catctccacc ctaaagcggc ggctcggcag atcaccctca
      181 cacacccgat atttgctgcc atcccagaca atcaggctca tgcgaggatc gtcagacagc
      241 aaacggatag cggtatattg atcaaacgta tcggtctggt agccattgag agcaacgatt
      301 acatatccct tgcgcaggcc aaaaggaacc atcgcctggc tggatccgct gatcagcacg
      361 cccctgatcg gcgcggaaga aaagtgtttt agcaacacct tacgcgcgcc ttccggaaac
      421 acctttttca ctttgtcttc ataccccagg gcaatggcca tgtccggaag aacctcatac
      481 attcgaaaca gacaagccga ctcattaacc ggctgatcgt cttcgtaacg tttgtcaatt
      541 ttccgagcgg tttccaatgc ctcctcccat tttccttccc gctccttcag ccaaatgaag
      601 gcctccaatc cattatagga ataaacatcg gcggcgtccg ccgcgacttt attagccatt
      661 tccaccccgc ctttttcaaa gagatatttc acaagccatt tggatctgtt cgccatccaa
      721 acccgatccg tagcctgctc aaacgctttc aaataggctt ccgccgcctt ttccggaagg
      781 tcttggccaa ccagataatt tcccagaatc aggtagcaat ttggatcgag cttcgctgcc
      841 ttctccatcg cttccgcata aagggctggc tggccttcgt cacagtcggc caagcgcctc
      901 attacttgga gattatagtc ggcaagcgga ccagcccatt ttcccaaaat ttccgcgctc
      961 agattggcaa agctccggtc attctccaaa taagcgttct gcaaagcgat cggataagaa
     1021 taaggagccc gctcccaaag cgttttcatc cacgcatcat tgttttcgtc accgacacca
     1081 attttataaa gccgggaacc ggattcgaag gcggttccgt aaggcacttc cggatgaaac
     1141 cacgaatgaa aatcaggaaa tgacagatgc aggtcgttgc cgtcctgatc cgttcgcagg
     1201 gaagaccaga gcgcgggggt gaccattgac ggctgatcat gaataagagc cgtgcaaacc
     1261 gcgttggcag aaatccgcgc cggattatcg cgggcgatca ttcgaatcag gaacggcttg
     1321 tatttgagtt ccggcaattt ttgtttgatg aactcttcga gtccagatgc ctgatccgtc
     1381 actccccatt gatcgttcaa aaacgtgaaa agagaattca gcccctgcat caaatttcgt
     1441 tgactgtaac ccgcaaacag gtttttcccg gcaacctgaa tcaccggttt tccgttctcc
     1501 atcagcaccg gcgatccctc cggcgcatcc agataatcct taagccagct taggtcatcc
     1561 ttggacggct tgatcccaaa ctcacgggcc gcctccgcgg tttcttcaaa ctcaacttcc
     1621 agaatgggtt tgctaaacgt atggccatcg gagacggata gcgaagattc attggcaatc
     1681 cgccagagag ccgggtcagg ttgaatcttg gcttcccgaa gaaaatcgag gcctgcggta
     1741 gcgttgatcg ccttcacaag agcgcgaaaa tattcccgct taagcgccgg tgagccgtgc
     1801 agggccgcgg accggctctc ccgccaatcc tgggtattgc ggaggcggaa aatttcctgc
     1861 cagtcggcca agatggcctc gaaaccttgg ggaggtttcc aggttttcag cgcggcaagg
     1921 gcatccgcct gctgcccgca gagggtcagg cgaagaacct ccacgaattg acggttcgga
     1981 tggtcgcgcg gaacgcccag agcatccgcc gcagccagat gagcaatcat tcggttaagg
     2041 ggaattcgaa tgtcccggaa atcaccggag tagtcgttca gggcaatcgt gccgaggaga
     2101 aatgtggcct gaagatgacc ctgccccgag tttgggtgag cctgcagaaa gctcgatatc
     2161 cgggcatttt ccttgaagta aattgactga gaagaagaga gaagcgtgcg ggccagttcg
     2221 tttatctcca actccgggga agccggatcc ggcttcccaa aaaaagcttt ggccagaggc
     2281 tgttaacttg cggggttcca taccccagcc gccagggaga tcgcggttgt ttttccatcc
     2341 cattggatct ggtaagtttc ggaagcatcc gccccgctgg caggctgggc agacaatggc
     2401 cgcatttttt tgccgttggc gagcgctcca taatccgaga gaaattgagc gacctgggtc
     2461 actgtccatc cctcttcggt ttgataggga agcgaaacat tcaccaccgc tggcggctcc
     2521 gcctgggtgt tttgcccgcg gaattgcaaa tatccaattc caaggggaat caaaaccgcg
     2581 aatgcaataa tcgggaccgc aacaagcgtc ggaattttca tggggcggga tgctaaaaag
     2641 cactacgatg ccaaaaggat ttgctgccgt aaagcagaca acccaattct tttagccgga
     2701 tcggcataga gccgaacgaa ggcttattac tcctgctccg ccagccagcg ctccgcgtcg
     2761 atggccgccg cgcaaccctg gcccgcggcc gtgatcgcct ggcggtaaac atggtccgcc
     2821 acgtcacccg cggcaaagac ccccggcagg ttggtgtggg tgccattccg ttgcag
//
LOCUS       DATDAY010000241         4735 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_1801102, whole genome shotgun sequence.
ACCESSION   DATDAY010000241 DATDAY010000000
VERSION     DATDAY010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 4735)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4735)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4735
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1801102"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..933
                     /locus_tag="VIS74_04795"
     CDS             <1..933
                     /locus_tag="VIS74_04795"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEY5792592.1"
                     /translation="DACPLAILMLDGGLRVTTWNPGAEKLLGWKREEIIGRPLPIPPN
                     LTRPFFAAIRHRQPVRLDAHWEAKSGGRLDLAIWYAPLCETPEKLPGHVSLLMDVSEK
                     HFLEKALLKESEREQRRIGKELQDHLAQYLLGAAFAVKALEGDLQREGSASAARLDEL
                     VRLLNDAVRQVQDISRGLHPVALDPTGLMFSLRELAERLNRSIPCQFLCENAVLIHDP
                     QVALHAYRIAQDAAFFALRAAGGKKITLSLAQKSSTVTLEVRKHGPPGIPKLPADETS
                     LKIMEYRAKAIDGHFAIHTQPRSGLRLTCAFPNA"
     gene            938..1621
                     /locus_tag="VIS74_04800"
     CDS             938..1621
                     /locus_tag="VIS74_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY5792593.1"
                     /translation="MSAKTSPASPPPASRVLIVDDHPVFRHGIAALINGESDLEVCGD
                     AFDSLTALDAMRRLEPDVALVDISLPGTNGIELVKLMKAERPKLPILVLSMYDESLYA
                     LRALRAGALGYVMKAEALGQVLSGLRKVLKGEIYVSPKFSEQLIFKAVQSIDGGMGSP
                     VDKLSDREFEVLELIGQGFGTREIAEKLHLSVKTIETHRAHIKEKLDFKDASEMVRFA
                     IDWVAQEKH"
     gene            complement(1772..2194)
                     /locus_tag="VIS74_04805"
     CDS             complement(1772..2194)
                     /locus_tag="VIS74_04805"
                     /inference="COORDINATES: protein motif:HMM:NF016045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity iron permease family protein"
                     /protein_id="HEY5792594.1"
                     /translation="MNRPRRTSLFTRFAKGTAHAAGRPATFAAAGGVLILWAVSGPFF
                     GFSDTWQLVINTGTTIVTFLMVFLIQNTQNRDSEAMHVKLDELIRSHQTAHNALLDLE
                     ELEEEELDRIRADYEKLAEAARGELHRRKHPAANRKPA"
     gene            complement(2208..2468)
                     /locus_tag="VIS74_04810"
     CDS             complement(2208..2468)
                     /locus_tag="VIS74_04810"
                     /inference="COORDINATES: protein motif:HMM:NF040452.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6515 family protein"
                     /protein_id="HEY5792595.1"
                     /translation="MKTPSSLLTLAGIAGMSLLITSCINVQTRPVPETVVTTTTYRPG
                     YVVTTLPVGYQTRVYRGTSYYYHRGTYYRPSGTRYIVVREPL"
     gene            2780..3373
                     /locus_tag="VIS74_04815"
     CDS             2780..3373
                     /locus_tag="VIS74_04815"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY5792596.1"
                     /translation="MTLDHSALMEDPQIMAALVKHEPSAIECLYLRYKSFLKSIIMQV
                     VHDECEADDVIQDVLIQVWNRPQTYSSEKGKLGSWLATLARRRAMDRIRKHCAYRRAT
                     DRYEVACNHPDKAVSEIHVVEAEADANDLKQLLEHYLETLPPSQQEVIRLAYFQSRSQ
                     REISLLTHTPLGTVKTRIELGIKKLTHAMGGSKRKIA"
     gene            complement(3690..4511)
                     /locus_tag="VIS74_04820"
     CDS             complement(3690..4511)
                     /locus_tag="VIS74_04820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792597.1"
                     /translation="MKPRSLPFRIRAWAAPFILAALAGGALSAFGAGEFGSTESSETA
                     LIGTFYDLKQTQKLKPTGMGKEEYVTTLGAFLKDGWDESALNRFFRASQPLYTTQIFI
                     PTMNADTAPKAFGVEKIVQPKMWLAHYKGQVVPPSTGRWRFWGMGDDILCVAVNGKIV
                     LVNNWGGLRFPGIPWKSVEPGQTFGKGRLMAGDWMDLKAGEPVDLDILIGELPGVLFA
                     SFLLIEKQGESYEMVNGWPAFPIFQLAPYQTPIPTDPKSTFPRFAPDGPIWKGVQ"
     gene            complement(4529..>4735)
                     /locus_tag="VIS74_04825"
     CDS             complement(4529..>4735)
                     /locus_tag="VIS74_04825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792598.1"
                     /translation="VNGQEQLAVHNAREPVVTREDGAGTFFWGGVHPNGGETSGIGGP
                     FFLTKGPLTQKACAAIEEQWKAFH"
BASE COUNT         1001 a         1467 c         1348 g          919 t
ORIGIN      
        1 gatgcctgtc ccctcgccat tttaatgctc gacggcggcc tccgcgtcac cacctggaat
       61 ccgggcgcgg aaaaattact cggctggaaa agggaggaaa taatcggccg cccgctcccc
      121 attccgccga atctcacgcg gccatttttt gccgccatcc ggcatcggca accggtccgc
      181 ctggacgcgc attgggaggc caagagcggc ggccggctgg atctcgccat ttggtatgcg
      241 ccgctctgcg aaactccgga aaagctcccc ggccatgtaa gcctgctcat ggatgtctcc
      301 gaaaaacatt ttctggagaa ggcattgctg aaggaaagcg agcgcgagca gcggcggatc
      361 gggaaggaac tgcaagacca tctcgcccaa tatctgctcg gcgccgcgtt tgcggtgaag
      421 gcgctggaag gcgatctcca gcgggagggc tccgcttcgg cggcgcgctt ggatgaactg
      481 gtccggctgt tgaatgatgc cgtgcgccag gtccaggaca tctcccgtgg actgcatccc
      541 gtggcattgg atcccacggg gctcatgttt tcgctgcggg aactcgccga acggctgaac
      601 cgctccattc cctgccagtt tctttgtgaa aacgccgtgc tcatccacga tccccaagtg
      661 gccctccacg cctaccgcat cgcccaggac gcggccttct tcgccctgcg ggccgccggc
      721 ggcaaaaaaa tcacgctgtc cctcgcgcaa aaatcttcta cggtaacctt ggaagttcgg
      781 aaacacggcc ctccggggat ccccaaactt ccggcggatg aaacctcgct caaaatcatg
      841 gaataccgag ccaaagccat tgacggccat tttgcgattc atacccagcc gcgctcggga
      901 cttcgcctga cctgcgcctt tcccaacgcc tgacctcatg tccgccaaga cttcccccgc
      961 cagccctccc cccgccagcc gggtgctcat cgtggacgac catccggtct ttcgccacgg
     1021 gatcgccgcc ctcatcaatg gggaaagcga tctggaagtc tgcggcgacg cctttgattc
     1081 cctcaccgcg cttgacgcca tgcgccggct ggagccggac gtggcgctgg tggacatttc
     1141 cctgcccggg accaatggca tcgaactggt caagctgatg aaggcggaac ggcccaaact
     1201 gccaatcctc gtcctgtcca tgtatgacga atccctttac gccctgcggg ccctgcgggc
     1261 gggcgcgctc ggttatgtca tgaaggccga agccctcggc caggtgcttt ccgggctgcg
     1321 caaggtgctg aagggggaaa tctacgtcag tcccaaattt tccgagcaac tgatcttcaa
     1381 agccgtgcag tccatcgacg gcggcatggg ttcgccggtg gacaaacttt ccgatcgcga
     1441 gttcgaagtg ctcgagttga tcggccaggg atttggcacc cgggaaatcg ccgaaaagct
     1501 gcacctgagc gtgaagacaa tcgaaaccca ccgcgcccac atcaaagaaa aactcgattt
     1561 caaggacgcg agcgaaatgg tgcgcttcgc catcgattgg gtcgcccagg aaaaacactg
     1621 attcgcccac gcgaggagcg ggcaagggga aaatcatccg ccgatttcgc cgattacgca
     1681 gattttttgg attccgttga tttcttactc tgcgaaatcg gcaaaatcgg cggatcccat
     1741 tccatggccc gccaaacggg cgtccaatga atcaagccgg ttttcggttc gccgcggggt
     1801 gctttcgccg gtgaagctcg ccgcgggccg cctccgccag cttttcgtaa tcggcccgga
     1861 tgcggtccag ctcctcttcc tcgagctctt ccagatcgag cagggcgttg tgcgccgttt
     1921 ggtgggagcg aatcaattcg tccagcttga cgtgcatcgc ttcggaatcg cggttctgcg
     1981 tgttctgaat gaggaaaacc atcagaaagg tgacaatggt ggtgcccgta ttgatgacca
     2041 gctgccaggt atcggaaaag ccaaagaacg ggccggagac ggcccatagg atcaggacac
     2101 cccccgcggc cgcaaaagtg gcgggacgcc cggccgcatg agccgtcccc ttggcaaatc
     2161 gcgtgaagag cgaagtccgg cggggcctgt tcaccgtggc aaaccgatta gagcggttcc
     2221 ctcacgacga tgtaacgagt gccactgggg cgataatagg tgcccctgtg ataataataa
     2281 ctggttcctc gataaacccg ggtttgatac ccgaccggaa gcgtcgtcac gacatagccg
     2341 ggccggtaag tggtggtggt caccaccgtc tccgggaccg gacgggtttg aacgttaatg
     2401 cagctggtga tcagcaggct cattccggcg attccggcca gagtgagcag ggaggatgga
     2461 gttttcatga tgggtaaaga agtccgcttt taatgaattc gccgggcgaa aaagctgtcc
     2521 cggcggcaat tagtaagact aaaaaccttc atgcggagct ggattcaaaa aggaatcgca
     2581 tcccacggcc gcttcgcgcg tatggcggcc atttattccc tctccgccta tcgcccggag
     2641 gatcagggga ttcccccatg cccggttcat cgtccccacc gatttcaagc caaggataag
     2701 ccggttaaat taattgcaaa aatcacatcc gctttttctc ccgcggagta aatttaagtc
     2761 aaccccccta attatggaaa tgaccctcga tcattcagcc ctgatggaag acccgcagat
     2821 catggccgcg ctggtgaaac atgaaccttc ggccatcgaa tgcctctacc tccgttataa
     2881 atcattcctg aaatccatca tcatgcaggt cgttcacgac gaatgcgaag cggacgacgt
     2941 gattcaggac gtattgattc aggtttggaa tcgcccgcag acgtattcct cggaaaaagg
     3001 caagctgggc agctggctgg ccacccttgc ccgccgccgg gccatggatc gcatccgcaa
     3061 gcactgcgcc taccgccggg ccacggatcg ttacgaagtg gcgtgcaatc accccgacaa
     3121 ggcggtttcg gagattcacg tggtcgaggc ggaagccgac gccaatgatc tcaagcagct
     3181 gcttgaacat tacctcgaaa ccctcccgcc cagccagcag gaggtcattc gcctggcgta
     3241 tttccaaagc cgaagccagc gggagatttc cctcctgacc cataccccgc tgggcacggt
     3301 caaaacgcgc atcgaactcg gcatcaaaaa actcacccat gcgatgggcg gatcgaagcg
     3361 caagatcgcc tgatcccccg catgccaccg catttttccg tttcacggac gggccggagg
     3421 cttggataat ccccggagcc ttttcgattt aattgtacgc atctccccgg cggggttggc
     3481 aaccccgccc caccgcgaac cgatctcccg gtggggcggc gtggccaacg ccgccgctca
     3541 tggcggcaaa ctgaatcgaa tagggttttg agaaccgctt aaagatttgc ccgccaccaa
     3601 ttcaggttga attcatttca atcgaaaaac agctctcgcg cggaccgcga agcgcttttt
     3661 ccgattggcc ggaaggaaaa ccagggaggt cactgcacgc ccttccaaat cggaccgtcg
     3721 ggcgcgaagc gggggaaggt ggacttggga tccgtgggaa tgggcgtctg gtagggagcc
     3781 agctggaaaa tggggaaggc cggccagccg ttcaccattt cgtaggactc cccctgtttc
     3841 tcgatcagca ggaatgaggc gaacagcacg ccgggaagtt cgccgatgag gatgtccagg
     3901 tccaccggct cgccagcctt caaatccatc caatcccccg ccatcaaccg gcccttgccg
     3961 aaagtctgcc cgggctccac agacttccac ggaatgccgg gaaaacgcag gccgccccaa
     4021 ttgttgacga gcacgatttt cccattcacc gcgacgcaga gaatgtcatc gcccatgccc
     4081 cagaagcgcc accggccggt cgaaggcgga acgacctggc ccttgtaatg cgccagccac
     4141 atcttcggct gcacgatctt ctcgacgccg aaagccttcg gcgcggtatc cgcgttcatg
     4201 gtcggaatga aaatctgcgt cgtgtaaagc ggctgggacg cgcggaagaa gcgattcagc
     4261 gcgctctcat cccagccatc cttcaaaaag gcgccgagcg tcgtcacgta ttcttccttg
     4321 cccatgccgg tgggcttgag tttttgcgtc tgcttgaggt cgtaaaaggt gccgatcaac
     4381 gccgtctcac tgctctcggt ggagccaaat tcgcccgcgc caaaggcgct caacgcgccg
     4441 ccggccaggg ccgccagaat gaagggagcc gcccaggcgc gaatccggaa agggagggaa
     4501 cggggtttca ttgaaagttt cgagggggtt aatggaacgc tttccactgc tcttcaatcg
     4561 ccgcgcaggc cttttgcgtc aacggtcctt tggtcagaaa aaatgggccg ccgataccgc
     4621 tcgtctcgcc gccgttcgga tggacgcctc cccagaaaaa agttccggcc ccgtcttccc
     4681 gggtcaccac cggttcacgc gcattgtgga ccgcaagctg ttcctggccg ttgac
//
LOCUS       DATDAY010000242         2078 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_1218910, whole genome shotgun sequence.
ACCESSION   DATDAY010000242 DATDAY010000000
VERSION     DATDAY010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2078)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2078)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2078
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1218910"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..337)
                     /locus_tag="VIS74_04830"
     CDS             complement(<1..337)
                     /locus_tag="VIS74_04830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792599.1"
                     /translation="MERIPRLNDEIQGQIELQPVETLRVKAGRDRVIEDILAILYICT
                     FMRGKIRAADPYCHVAPRKNPRDVRRKTCRLGILENCLITYLDLHARSPIRFIVTFTR
                     MFWAPDPLNV"
     gene            359..925
                     /locus_tag="VIS74_04835"
     CDS             359..925
                     /locus_tag="VIS74_04835"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HEY5792600.1"
                     /translation="MELPFQYPPREAEVVAPCLKRRSPDCIFPDPLRPEGKRPTVESL
                     VALNSWLHENIAYERREEGDPLPVEETLAKAKGSCRDFSVLMAEVLRFHGVAARLVSG
                     FLWEGAVEERERRAENALHAWVEAYLPGAGWIGMDPTNGVFCDHHFLPTAIGLAPSDI
                     APITGHYYGNKTIASTLETKLTIGENKS"
     gene            937..>2078
                     /locus_tag="VIS74_04840"
     CDS             937..>2078
                     /locus_tag="VIS74_04840"
                     /inference="COORDINATES: protein motif:HMM:NF021424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HEY5792601.1"
                     /translation="MDKACARAEQIFTRNTVRLTLGGEPTYVPLNPEGAEWSYSAVGP
                     TKLAYAWKVAAELQKTRMPGAAAFYCPGKSYPGEVNPRWVIKLLANRNGTPLYRSPVR
                     GRTPTTQTLQQFADGICKTLGVPSHWLALSDPQNPRTEVLAMPLDHDKAIWRSARWPL
                     EKKSRVLSEAEGPAGLRLPLNLLPPSLPRRALTVERTGNTIAIFFPPLLQKPFLELLS
                     AVEIAGRKAGIARVNLQGYIPSDEACQWTSLGLAADPGVLEINLPACVSWRDYAGWIE
                     SVTDAAEKCGLRSWKENLWEPPQGTGGGNHLLWGGPSLEEHPFFTRPAWLASILRYWQ
                     HHPALGYLFTGCYVGASSQAPRPDESARDLYDIEMAYSFLESLPEG"
BASE COUNT          411 a          625 c          623 g          419 t
ORIGIN      
        1 gaacgttcaa ggggtccggc gcccagaaca tgcgagtgaa ggtgacgatg aaacgaattg
       61 gggaacgggc atgaagatcg agatacgtta tcaggcagtt ttccaatatg ccgagccggc
      121 aagtttttcg ccgcacatcg cgcggatttt tccgcggcgc gacatggcag taagggtcag
      181 ccgcgcggat ttttccacgc atgaaagtgc agatgtacag aatcgccagg atgtcttcga
      241 taacccgatc gcgacctgct tttacccgga gcgtctcgac cggctggagt tcgatttggc
      301 cctggatctc gtcattgagc cgcggaatcc tttccatttc ctggtagatt cgtccgccat
      361 ggaattgccg tttcagtacc caccgcggga ggcggaggtg gttgcgcctt gcctgaaacg
      421 ccgctctccc gactgcattt tcccggaccc tttgcgcccc gagggcaaac gccccaccgt
      481 ggagtcgctg gtcgccctca acagctggct gcatgagaac atcgcttacg agcggcgcga
      541 ggagggcgat cccctgccgg tggaagaaac gctcgcgaaa gctaagggat cctgccggga
      601 cttttccgtc ctgatggcgg aagtcctgcg gttccatggc gtggccgccc ggttggtcag
      661 cggatttctc tgggagggcg cggtcgaaga aagagagcgg cgggcggaaa atgcgcttca
      721 tgcctgggtg gaggcctatt tgcctggcgc cggctggatc ggcatggatc ccacgaacgg
      781 tgtcttttgc gatcatcatt tccttcccac ggcaatcggc ctcgccccgt ctgatatcgc
      841 gccgatcacc ggccattatt atggaaacaa gaccatcgcc agcacgctgg agaccaaact
      901 aacgatagga gaaaacaaat catgatcgct ccctggatgg acaaagcttg cgcccgagcg
      961 gagcagatat tcacgagaaa cacggtgcgg ctcactctcg gaggggagcc cacttacgtt
     1021 ccgctcaacc cggagggcgc cgagtggagc tattccgccg tgggccccac caaacttgct
     1081 tatgcatgga aggttgcggc ggaactgcaa aaaacgcgca tgccgggcgc agcagcattt
     1141 tactgcccgg gcaaatccta ccccggggaa gtcaatccgc gctgggtaat caaattgctc
     1201 gccaaccgca atggcacccc gctctaccgg tcgccagtcc ggggccgtac tccgaccacc
     1261 cagacgcttc agcaatttgc cgacggcatt tgcaaaactc tcggcgtgcc ctcccattgg
     1321 cttgcgttga gcgatccgca gaatccgcgg acggaagtgc tcgccatgcc gttggatcac
     1381 gacaaggcga tttggcgatc ggcccgctgg ccgctggaga aaaaatcccg cgtgctttcc
     1441 gaagcggaag gtcccgccgg ccttcggctg ccgctgaact tgctgcctcc gtcgctgccc
     1501 cgccgggctc tcacggtgga acgcaccggc aacacgatag cgatcttttt tccaccgctc
     1561 ctgcaaaagc catttctgga actgctttct gcggttgaga tcgcgggaag aaaggcgggc
     1621 attgcccgtg tgaatctaca gggttacatt ccttcggacg aggcctgtca atggacgtcg
     1681 ctcggactgg cagccgatcc gggcgtgctc gaaatcaatt tgccggcttg cgtgagctgg
     1741 cgcgactacg cgggctggat cgagtcggtg accgatgctg ctgaaaagtg cggactccga
     1801 tcctggaagg aaaatctctg ggagccccct cagggaaccg gcggcggaaa tcacctcctt
     1861 tggggcggac cgtctttgga agaacatccg ttcttcacgc gtcccgcctg gcttgcctcc
     1921 atcctgcgat actggcagca tcatccggcg ttgggctatc ttttcacagg ctgttatgtg
     1981 ggcgcttcct cccaggcgcc ccggccggat gaatcggcaa gggacctcta cgacatcgag
     2041 atggcctaca gttttctcga atcactgccg gaaggcga
//
LOCUS       DATDAY010000243         4577 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_567157, whole genome shotgun sequence.
ACCESSION   DATDAY010000243 DATDAY010000000
VERSION     DATDAY010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 4577)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4577)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4577
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_567157"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1011)
                     /locus_tag="VIS74_04845"
     CDS             complement(<1..1011)
                     /locus_tag="VIS74_04845"
                     /inference="COORDINATES: protein motif:HMM:NF025086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HEY5792602.1"
                     /translation="MKTFLEKISRSRDLTVSVLLHVVILLALAATVAVNVVTDPPPDV
                     ISPGPLVQNDPAPARPVDPVPDRESQINPLKSWQPSSPADLAPEIIRTQRPAPTEYTV
                     PIGQPAPMLPEMAAGISRLSERPLAEGIPADVRAAIRESIQQWQQGGGKYHFTAALAQ
                     YEGGDWNSTVRVRNQRVVGGSLPNLLYLMNKWSKDRITTNEDKVRIMRLDSGEILTQR
                     PPFIFMTGTRDFKLTDAEVENLRKYLRLGGCVWGDSSVPGLRSRFDLAFKREMKRVIG
                     NVDAEFENLPANHRLFREAYFKPVNSAPPGLNFYREPVSVLKMFGEVAVIYTANDYGD
                     MWQ"
     gene            complement(1040..2356)
                     /locus_tag="VIS74_04850"
     CDS             complement(1040..2356)
                     /locus_tag="VIS74_04850"
                     /inference="COORDINATES: protein motif:HMM:NF025086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HEY5792603.1"
                     /translation="MPLPFQNYFQKLLDKFSTSRDFTISFFLHLLLVVIFGTTVLFQA
                     VTEPPDFEGESGGFVAAEENNTPQEPVTQPQQTTFNVQAVTPPQTSTPLTAITTSAQN
                     PLNFSMSATTIPSPVSNTPTEISQSVAAPKAPSVSGDQMTTAQASQIKSFTGGWGKGT
                     GSGTGLRQREFEFTAYIGQYQGGNWNSTVRVQKDKITGGSLPNLLYIINKWSKDKIKT
                     NDKNVVALKLDSDEIFAKKPPFIFLTGTRDFKLTDQEVENLQKYVRSGGCIWGDSSLP
                     GLRSRFDIAFRREMRRVIPDVDKDFEPLPKDHPIYTKNYYPEIKETPPGLNYYREPIF
                     ALKIYGEIAVIYTANDYGDMWQIGLTDKGEVDLRRNQNNEFVAINPGIWASRNSYLGN
                     ISPPPGKKPNEPVVATNLVDTYKFGTNIILHLLTRWESKTARAPDL"
     regulatory      2450..2551
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            2650..>4577
                     /gene="thiC"
                     /locus_tag="VIS74_04855"
     CDS             2650..>4577
                     /gene="thiC"
                     /locus_tag="VIS74_04855"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HEY5792604.1"
                     /translation="MIATQETIEPQSTDQLPNSTRVYVQGELFPGVRVPMREIELSPT
                     KSFNGQLEPNAPVRVYDTSGPWGDPEFDGDVELGLPALRHSWILQRGDVAEYEGRKVK
                     PIDNGYLSEVHALARNGNPKSKIQNPKFARTPLRASAGHPVTQLWYAKQGIITPEMEF
                     IAIRENQNVAQASSLWSKNDHRLEADATFARNDLRHRHEGSQTRNDGFQPGIFGRFPQ
                     RIPKIITPEFVRSEVAAGRAIIPANINHPELEPMIIGRNFLVKINANIGNSAVASSIE
                     EEVEKMRWATKWGADTVMDLSTGKNIHATREWILRNSPVPIGTVPIYQALEKVSGKAE
                     ELTWEIYRDTLIEQAEQGVDYFTIHAGVLLRFIPLTARRMTGIVSRGGSILAKWCLAH
                     HKENFLYTHWDDICDIMAAYDVSFSIGDGLRPGSIADANDQAQFGELEVQGELTKRAW
                     AKGVQVMNEGPGHVPMHMIEENMAKQLEWCHEAPFYTLGPLTTDIAPGYDHITSGIGA
                     AMIGWYGCAMLCYVTPKEHLGLPNKQDVKEGVITYKIAAHAADLAKGHPGAQYRDNAL
                     SKARFEFRWEDQFNLGLDPERAREYHDETLPQEGAKTAHFCSMCGPHFCSMKITEDVR
                     KYAAEQAISEEEALKKGM"
BASE COUNT          932 a         1449 c         1320 g          876 t
ORIGIN      
        1 ttgccacatg tcgccgtagt cattggccgt gtaaatgacc gccacctcgc caaacatctt
       61 caacacggaa acgggctcgc ggtaaaaatt caaccccggc ggcgccgagt tgaccggctt
      121 gaaataagcc tcgcggaaaa gccggtgatt ggccggcagg ttctcgaatt ccgcgtccac
      181 attcccaatc acccgtttca tctcgcgctt gaaggccagg tcaaaccgcg agcgcaggcc
      241 cggcaccgag ctgtcgcccc agacgcagcc gcccaggcgc aggtatttcc gcaggttctc
      301 cacttcggca tccgtcaatt tgaagtcccg cgtgccggtc atgaaaatga agggcgggcg
      361 ctgggtgagg atttcgccgc tgtccaggcg catgatccgc accttgtcct cgttcgtcgt
      421 gatccggtcc ttggaccatt tgttcatgag gtagagcaaa ttcggcaggc tgcccccgac
      481 cacccgctga ttccgcacgc gcacggtgga attccagtcg ccgccctcgt attgggctag
      541 cgcggcggtg aaatggtatt tgccgccgcc ctgctgccat tgctggatgg attcgcgaat
      601 ggcggcgcgg acgtcggcgg gaatcccctc ggcgagcggg cgctcgctga gccgggaaat
      661 tccggcagcc atttccggca gcatcggcgc gggttgcccg atcgggacgg tatattccgt
      721 gggagccggc cgctgggtgc gaatgatctc cggcgcgagg tccgccgggc tggagggctg
      781 ccagcttttc agcggattaa tctggctctc ccgatccggg accggatcca ccggccgggc
      841 cggagccggg tcgttctgca ccagcggacc cggcgatatg acatccggag gcggatcggt
      901 gacgacgttg accgcgacgg tggccgccag cgccagcagg atcaccacgt gcagcaggac
      961 ggataccgtc agatcccgag aacggctgat tttctcgaga aaggttttca tggagggatt
     1021 tgggggcgcg gagggggcgt tacagatccg gcgcccgcgc ggtcttgctt tcccagcggg
     1081 tcagcaggtg gaggatgatg ttcgtgccaa acttgtaggt gtccaccaga ttcgtggcga
     1141 ccaccggctc gttgggtttt ttgcccggcg gcggggagat gttgccaagg tagctgttcc
     1201 gggatgccca gatgccggga ttgatcgcca cgaattcgtt attctgattc cgacgcaggt
     1261 ccacctcgcc cttgtccgtc aggccgatct gccacatgtc gccgtagtca ttggccgtgt
     1321 aaatgacggc aatctccccg tagattttca gggcaaagat cggctcgcgg taataattca
     1381 accccggcgg cgtttccttg atttcgggat aataattctt cgtgtaaatc gggtgatcct
     1441 tcggcagcgg ctcgaagtcc ttgtccacat ccggaatcac gcggcgcatc tcgcggcgga
     1501 aggcgatgtc aaagcgcgac cgcaggcccg gcagcgagct gtccccccag atgcagccgc
     1561 cggagcgcac gtatttctgc agattttcca cttcctggtc ggtcagcttg aaatcccgcg
     1621 tgcccgtcag gaaaatgaag ggcggctttt tggcaaaaat ctcgtcgctg tccagcttca
     1681 gcgcgaccac gtttttgtcg ttggtcttga tcttgtcctt cgaccacttg ttaatgatgt
     1741 aaagcaggtt gggcaggctg ccgccggtga tcttgtcctt ttggacccgc acggtggagt
     1801 tccaattgcc gccctggtat tggccgatat aagccgtgaa ttcaaattcc cgctggcgga
     1861 gcccggtgcc gctgcccgtg cctttgcccc atccgccggt aaaggacttg atctgcgaag
     1921 cttgggcggt ggtcatctga tccccgctga cggatggcgc tttcggcgcc gccacggatt
     1981 gggaaatttc cgtcggcgtg ttcgagaccg gcgagggaat cgtggtcgcg ctcatggaaa
     2041 agttgagcgg attctgcgcc gaggtggtga tggccgtcag cggcgtgctg gtctgcggcg
     2101 gcgtgaccgc ctggacattg aaggtcgtct gctgcggctg ggtcaccggc tcctgcgggg
     2161 tgttattttc ctccgcggcc acaaaccccc cgctctcccc ctcgaaatcc ggcggttcgg
     2221 tcaccgcctg aaacagcacc gtggtgccaa agatcaccac caggaggaga tgcaggaaaa
     2281 aggaaatggt gaagtctctc gaagtgctga acttatcgag cagcttctga aagtaatttt
     2341 gaaacgggag gggcaccccg gaattttcct tcagccccct ggcgcttgca atcaaaaaac
     2401 accatcgaag cgaacggaat attgaccatt tcccctcggc ggtgtttact ttttcccaca
     2461 ggggagccgc gaggctgaga gtcccgttcg cgggagaccc tttgaacctg atacggatca
     2521 tgccggcgaa gggagaatta tgagtcttaa gttgccagtt ttaagtttta agacccccgc
     2581 aagtggatgc ggatatccgt tccctattga ccggcttaaa attttcaacc cacccgctta
     2641 aaactcgcca tgatcgccac ccaggaaacc atcgaaccgc agagcaccga ccagctcccc
     2701 aactccaccc gcgtttacgt ccagggcgaa ctcttccccg gcgtgcgggt gccgatgcgc
     2761 gaaatcgaac tctccccgac caagtccttc aacggccagc tcgaacccaa tgcccccgtc
     2821 cgcgtctatg acaccagcgg cccgtggggc gatccggaat tcgacggcga cgtggagctc
     2881 ggcctgcccg ccctgcgcca ctcctggatc ttgcaacgcg gcgatgtcgc cgaatacgag
     2941 ggccgcaagg tcaagccgat cgacaatggc tacctctccg aagtccacgc cctcgcccgg
     3001 aacggcaatc caaaatccaa aatccaaaat ccaaaattcg cccgcacccc gctgcgggcg
     3061 agcgccggcc atcccgtcac ccagctgtgg tatgcgaagc agggcatcat cacgccggag
     3121 atggaattta tcgcgattcg tgaaaatcaa aatgtggcac aggcttccag cctgtggtcc
     3181 aaaaacgatc atcggctgga agccgatgcc actttcgccc gcaacgacct tcgccaccgt
     3241 cacgaaggtt cccaaacccg caacgacggt ttccagcccg gcatctttgg gcggtttccg
     3301 cagcggattc cgaaaatcat caccccggaa ttcgtgcgct ccgaagtcgc cgcgggacgc
     3361 gccatcattc cggcgaacat caatcacccg gagctcgagc cgatgatcat cggccgcaac
     3421 ttcctcgtga agatcaatgc gaacatcggc aactccgccg tcgcgtcgtc catcgaagag
     3481 gaagtcgaga aaatgcgctg ggccaccaag tggggcgcgg atacggtcat ggatctctcc
     3541 acgggcaaaa acatccacgc cacccgcgaa tggattttgc gcaattcacc cgtccccatc
     3601 ggcaccgttc caatttatca ggcgctggaa aaagtcagcg gcaaagccga ggaactgacg
     3661 tgggaaatct atcgcgacac gctcatcgag caggccgagc agggcgtcga ttatttcacc
     3721 attcacgccg gcgttttgct gcgcttcatc ccgctcacgg cccggcgtat gacgggcatc
     3781 gtctcccgcg gcggctcgat tctggccaaa tggtgcctcg cccatcataa ggaaaacttc
     3841 ctctacaccc actgggacga tatttgtgac atcatggccg cgtatgatgt cagcttttcc
     3901 atcggcgacg gactccggcc cggcagcatc gccgacgcga acgaccaggc gcaattcggc
     3961 gagttggaag tccagggcga actcaccaaa cgcgcctggg ccaaaggcgt gcaggtcatg
     4021 aacgaaggcc ccggccacgt ccccatgcac atgatcgagg aaaacatggc caaacaactg
     4081 gaatggtgcc atgaagcgcc gttctacacg ctcggcccgc tgacgaccga catcgccccc
     4141 ggctacgacc acatcaccag cggcatcggc gccgctatga tcggctggta tggctgcgcc
     4201 atgctctgct acgtcacgcc caaggaacat ctcggcctgc ccaacaagca ggacgtcaag
     4261 gaaggcgtca tcacctacaa gatcgccgcc cacgccgccg acctcgccaa aggccatccc
     4321 ggcgcccaat accgcgacaa cgccctcagc aaagcccgct tcgagttccg ctgggaagat
     4381 cagttcaacc tcggcctcga ccccgagcgc gcccgcgaat accacgacga aaccctcccc
     4441 caggaaggcg ccaaaaccgc gcacttctgc agcatgtgcg gcccgcactt ctgctcgatg
     4501 aagatcaccg aagacgtccg caaatacgcc gccgaacagg cgatctccga ggaagaggcc
     4561 ttgaagaaag gcatgga
//
LOCUS       DATDAY010000244         6021 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_1414116, whole genome shotgun sequence.
ACCESSION   DATDAY010000244 DATDAY010000000
VERSION     DATDAY010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 6021)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6021)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6021
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1414116"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..656)
                     /locus_tag="VIS74_04860"
     CDS             complement(<1..656)
                     /locus_tag="VIS74_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006983674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY5792605.1"
                     /translation="MSDFIYPKTYDVIVIGAGHAGIEAALAAARLGCQTLILTQNADT
                     IGQMSCNPAIGGLAKGHMVREIDALGGIMGLNADATGIQFRMLNASKGPSVRAPRAQC
                     DKKAYQFRLKAILERTPGLDVKQANVTRLQVEGDAVSGVETHFGLTFLGKSVVVTTGT
                     FMRGLLHVGLRNQTGGRMGDTVSTFSDSLRELGFEIGRFKTGTPCRLLARSIDFSKCE
                     P"
     gene            complement(706..1140)
                     /locus_tag="VIS74_04865"
     CDS             complement(706..1140)
                     /locus_tag="VIS74_04865"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: protein motif:HMM:NF004064.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEY5792606.1"
                     /translation="MKIKQLMEFISLDSEIRELIQAAREARRMAHAPYSRFPVGAALL
                     AADGRIFPGVNVENLSYGLTMCAERVAIGTAVSAGIARFQAIAIVAETDEPISPCGAC
                     RQVMAEFGEALAVFSATLNGKVAQFSLAELLPRARTGILNQP"
     gene            1281..1562
                     /locus_tag="VIS74_04870"
     CDS             1281..1562
                     /locus_tag="VIS74_04870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792607.1"
                     /translation="MKSTIEELDQLRQLVRETVAAYTERLESEIGLVRSAVAELRKQK
                     EPTTERLHDLRDMLTLLRHFSVKADKGRRKDLKKIDSLVGDLRMMIENW"
     gene            1562..2164
                     /gene="msrA"
                     /locus_tag="VIS74_04875"
     CDS             1562..2164
                     /gene="msrA"
                     /locus_tag="VIS74_04875"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR00401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEY5792608.1"
                     /translation="MKFFLAAGVAFLAIWGMLLPMSAAADSTEILTVGGGCFWCTEAV
                     FEKFPGVKAVVSGYEGGSVPNPSYERVCTGTTGHAEVIQIEFDPKTAPLGKLLDLFFL
                     AHDPTSLNRQGADAGTQYRSVIFYSGDAQKAAALAAKERAQAELSKPIVTEISPTTTF
                     YPAEDYHQDYFQKNPHAGYCTFVIRPKIQKLEKSGVLNGK"
     gene            2216..3289
                     /locus_tag="VIS74_04880"
     CDS             2216..3289
                     /locus_tag="VIS74_04880"
                     /inference="COORDINATES: protein motif:HMM:NF033668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PA0069 family radical SAM protein"
                     /protein_id="HEY5792609.1"
                     /translation="MIRQSPSASHQSPAFPGRGSSISPANRFEKLSVELDEEFAAEAA
                     APKTQFLVDDTKSLITTNDSPDIPFRSSFNAYRGCEHGCAYCYARPYHEYLGFNSGID
                     FETKILVKLRAPELLRRELSSEKWQPQALAMSGVTDCYQPVERKLELTRRCLEVLLEF
                     RNPVGIVTKNALVTRDLDLLAPLAAFRCARVFLSITTLDRGLAQKLEPRAAMPRARLD
                     TVRQLAAAGVPVGVLCAPIIPGLNDHEIPALLEAAAEAGAGLAGFTILRLPYAVKDVF
                     SAWLDRHYPGHKEKILARVRDLRGGKLNDSRFGRRMSGEGFWAEQIEQLFEKCAARLG
                     LNRPAPPMTTEHFRRPREQLTLF"
     gene            3374..4000
                     /locus_tag="VIS74_04885"
     CDS             3374..4000
                     /locus_tag="VIS74_04885"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HEY5792610.1"
                     /translation="MEGSWTEFLYRTLDAIPGYGWVGWIWFIAIYSFTCIVFLPGSAL
                     TIGAGAIYGFWWGTLLVSIGNMVGATLSFLISRYLARGWMQKKLSHSKRFRALDRLMT
                     TDGWKMLFLSRLSPVVPHSIVSYAAGLSRMSLGKYILVSWVGFVPISAAYSYAGVLLR
                     QVTKASLGHPGTTPLGWVAYSLGAIVTIAVCVLSAKMVWKALGPRAES"
     gene            4010..4744
                     /gene="mtgA"
                     /locus_tag="VIS74_04890"
     CDS             4010..4744
                     /gene="mtgA"
                     /locus_tag="VIS74_04890"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: protein motif:HMM:TIGR02070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HEY5792611.1"
                     /translation="MSAKSGSSWKKRLLLGLGIALALILVLPVVHIALTKFWNIPFSP
                     MQAQRWIEAWTGGKSELSQPMRWLALDQIPRPLIHYIWASEDQRFFEHDGFDFGELQK
                     ALDEVMAGKKGAGRGASTITMQTARSVYLWQGRSYVRKALEAYYTIWMEQLLSKRRIL
                     ELYLNHLELGRGVYGVGAGSAVHFGKPPERLTNRQMMALAAVLPNPREWSPARPNAIV
                     QRKIRRIQRLSSNAPFPSDELPDKNE"
     gene            complement(4684..5565)
                     /locus_tag="VIS74_04895"
     CDS             complement(4684..5565)
                     /locus_tag="VIS74_04895"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY5792612.1"
                     /translation="MPVEETYHSDGAEFPYAAWRAKEEKARVYFLHGMGSAPREFAPL
                     GEFLSAAGVSLFAPALRGQGLDPEIRRRGSEVDVVRILGDLEAFLDQTPAVCPLFVSG
                     ESLGALLAIAALADSPKLQARFRGAVLLCPVVELRQKTPAVIVELIRWLARVIPRASL
                     HPGLFIHGKARAPKLTANTEYQQALAKAPFRLPQVCLGFYAGMARLMEGCLRAGPRIP
                     RPLLVLGGRNDPYISPDQLTSWFNSVSQPGCRLRFLPRSYHLLLRDPDCAEVMEEIYS
                     FLSGNSSLGNGAFEESR"
     gene            5628..>6021
                     /locus_tag="VIS74_04900"
     CDS             5628..>6021
                     /locus_tag="VIS74_04900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792613.1"
                     /translation="MKKFLFILLALTASTFAGQLDLAVVQYATAKDSAELAAGFANVD
                     LAEATNADSVIKGDNAIRGGSVIFAQTIPASPGSRLTTSTRLDARRVDVTTALNGLHL
                     TANISIITGVQQALRSYTERTYAAEGDLN"
BASE COUNT         1166 a         1982 c         1769 g         1104 t
ORIGIN      
        1 ggttcgcatt tggaaaaatc aatggaacgc gcgagcaggc ggcaaggggt gccggtttta
       61 aagcggccaa tttcgaagcc cagctcccgg aggctgtcgc tgaaggtgga aaccgtatcc
      121 cccatgcgtc cgccggtttg atttcgcaat cccacgtgca ggagtccccg cataaaggtg
      181 ccggtggtga ccaccacgga ttttccgagg aaggtgaggc cgaaatgggt ttccacgccg
      241 ctcacggcat cgccttccac ctgaagccgg gtgacgttcg cctgtttgac atccagaccg
      301 ggagtccgct ccagaatggc tttgaggcgg aattgatagg cctttttatc gcattgggcc
      361 cgcggcgcgc gcacactcgg gcccttgctg gcattcagca tccggaactg aatgccagtc
      421 gcatccgcgt taagccccat gatcccgccc agggcgtcga tttcccgaac catgtgcccc
      481 ttggccaggc cgccgatagc cggattgcag gacatctgcc caatcgtgtc ggcattctgg
      541 gtcagaatca aggtctggca acccaaacgc gccgccgcga gcgccgcctc gatgcccgca
      601 tggccggcgc cgatgacgat gacatcgtaa gttttcggat agataaaatc gctcatcggg
      661 cattctccca tcgccgggga tggatccatt gttccacgta gaacattacg gttggttcag
      721 gatccccgtc cgggcgcggg gcaacaattc cgcaagcgaa aattgcgcca ccttgccatt
      781 caaggtcgcg gagaagacgg caagcgcctc gccgaactcc gccatgacct ggcggcaggc
      841 tccgcaggga ctgatcggtt catcggtttc agcgacaatc gcgatcgcct gaaagcgggc
      901 gatgccggcg gagacggcgg tgccgattgc cacccgctcg gcgcacatcg tcaatccgta
      961 gctcagattt tccacattga ctcccggaaa gatccggcca tccgccgcga gcaaagcggc
     1021 cccgaccggg aagcgggaat agggagcatg cgccatccgc cgggcttccc gggccgcctg
     1081 gatcaactcc cggatttcgg aatcaaggga tataaattcc attaactgct taattttcaa
     1141 taaattataa caaccggccg cccaattatg gcgaattttt aacttgccgg aatcgaaaga
     1201 cttacgtcct cccttgcggt ttgacttcca tctgctttat tctgcctcta atgcatgccg
     1261 acggcaagga gacttctccg atgaagtcaa cgatcgagga gctcgaccag ctccgccagc
     1321 tggtgcgtga gaccgtggcg gcctacactg agcggctgga atccgaaatc gggctggtgc
     1381 ggagcgccgt ggccgagctg cgcaagcaaa aagaacccac caccgaacgc ctccacgatc
     1441 tgcgcgacat gctgacgctg cttcgccatt tctcggtcaa ggccgacaag gggcggcgca
     1501 aggacttgaa aaaaatcgat tccctcgttg gcgatttgcg catgatgatc gagaattggt
     1561 aatgaaattt tttctggcgg cgggcgtggc gtttctggcc atttggggta tgcttttgcc
     1621 catgagcgcg gcggcagatt ccacggaaat cctcacggtt ggcggcggtt gtttttggtg
     1681 cacggaggcc gtttttgaaa aatttcccgg cgtgaaggcc gtggtctccg gctacgaggg
     1741 cggctcggtg ccgaatccca gctacgaaag ggtctgcacg ggcacaaccg gccatgcgga
     1801 agtcattcaa atcgaatttg atccaaaaac ggcacccctc ggcaagctgc tcgacctttt
     1861 tttcctggcc catgatccga ccagcctcaa ccgccagggc gcggatgccg gcacgcaata
     1921 ccgttcggtg atcttttact ccggcgacgc gcagaaggcc gccgcgctgg ccgccaagga
     1981 acgcgcccaa gcggagctga gcaaacccat cgtcacggaa atctccccca ctacgacctt
     2041 ttatccggcg gaggactacc atcaggacta tttccaaaag aacccccacg cgggttactg
     2101 cacctttgtg atccgcccga agatccagaa gctcgagaag agcggtgttc tgaatgggaa
     2161 gtgactcgtg actagtgatt agtgaatggt cgagcgaacg cgtccttccg ctaaaatgat
     2221 tcgccagtca ccatccgcca gtcaccaatc acccgccttc cccggccgcg gcagttcgat
     2281 ttcgccggcg aaccggttcg agaaactatc cgtcgagctc gacgaggaat tcgccgcgga
     2341 ggccgcggcg ccgaagacgc agtttctcgt ggacgacacg aagagcctca tcaccacgaa
     2401 cgacagcccg gacattccct tccgatcgag ttttaacgcc taccgcggct gcgagcacgg
     2461 ctgcgcctat tgctacgccc ggccgtatca cgaatacctc ggcttcaaca gcggcattga
     2521 tttcgagacc aaaattctcg tcaaactgcg cgcgccggaa ttgctgcggc gcgagctttc
     2581 gagcgaaaaa tggcagccgc aggcgctggc gatgagcggc gtcaccgact gctaccaacc
     2641 cgtggagcgc aagctcgagc tcacgcgccg gtgcctggag gtgctgctgg aatttcgcaa
     2701 ccccgtcggc atcgtgacca agaatgcgct cgtcacgcgc gatctcgacc tgctcgcccc
     2761 gctggcggcc ttccgctgcg cgcgggtgtt tctgagcatt accacgctcg accgcgggct
     2821 ggcgcaaaag ctcgaaccgc gcgcggccat gccccgggcg cgcctggaca ccgtgcgcca
     2881 gctcgccgcc gccggcgtgc cggtgggcgt cctgtgcgcg ccgatcattc ccggcctgaa
     2941 cgaccacgaa atccccgccc tgctcgaagc cgccgcggag gccggggccg gcctggcggg
     3001 gttcacgatt ctgcggctgc cctacgcggt gaaggacgtg ttcagcgcgt ggctcgaccg
     3061 ccattacccc ggccacaagg aaaaaatcct ggcgcgcgtc cgcgatctgc gcggcggcaa
     3121 gctcaacgat tcgcgcttcg gccggcgcat gagcggcgag gggttctggg cggaacaaat
     3181 cgagcagctc ttcgaaaaat gcgccgcgcg cctcgggctc aaccgtccgg cgccgccgat
     3241 gaccacggag cacttccgcc gtccgcgcga gcagctcacg ctgttttaac cacccgcctt
     3301 cggggatttg gggcgcatgt ttttcctgtt aatcctgtca atcccggcaa tcctggctag
     3361 tttcgggagc cccatggaag gaagttggac ggagtttttg tatcgaaccc tggacgccat
     3421 ccccggctac ggctgggtcg gctggatttg gttcatcgcg atttacagtt tcacctgcat
     3481 cgtgttcctg cccggctcgg cgctgacgat tggcgcgggg gcgatctatg gattctggtg
     3541 gggaaccctg ctggtttcga tcggcaacat ggtcggcgcc acgctgagtt ttctaatcag
     3601 ccgttacctc gcgcgcgggt ggatgcaaaa aaaactctcc cacagcaagc gcttccgcgc
     3661 cctggaccgc ctcatgacca cggatggctg gaaaatgctt ttcctcagcc ggctttcccc
     3721 cgtggttccg cacagcatcg tgagctacgc ggcggggctg tcccggatga gcctcggcaa
     3781 atacatcctg gtgtcctggg tcggatttgt gcccatcagc gcggcttatt cctatgccgg
     3841 ggtgcttctt cgccaggtca ccaaggccag cctcggccac ccgggaacga cgccgctcgg
     3901 ctgggtggct tacagcctgg gcgccattgt gacgatcgcc gtctgcgtgc tcagcgcgaa
     3961 gatggtttgg aaagcgctcg gcccgcgggc ggaatcgtaa cctccggtca tgagcgccaa
     4021 gtccggttct tcgtggaaaa aacgccttct cctcgggctc ggcatcgcgc tcgcgctgat
     4081 tctggttttg cccgttgtcc acatcgcgct cacgaaattc tggaatatcc ccttcagccc
     4141 gatgcaggcc cagcgctgga tcgaggcctg gaccggcggc aaatcggaac tgagccagcc
     4201 gatgcgctgg ctggcgctcg atcagatccc ccgcccgttg atccactaca tctgggcctc
     4261 ggaggatcag cgctttttcg aacacgacgg ctttgacttc ggggaattgc agaaggcgct
     4321 ggatgaagtc atggccggca aaaagggcgc cgggcgcggg gcttcgacca tcaccatgca
     4381 aaccgcgcgc tcggtttatc tgtggcaggg gcgctcgtat gtccgaaagg cgctggaggc
     4441 ctattacacg atttggatgg aacagctgct cagcaagcgg cggattctcg aactctacct
     4501 caaccacctc gagctgggcc gcggcgttta cggcgtgggc gcgggatcgg cggtccattt
     4561 cggcaagccg cccgagcgtt tgacgaaccg ccagatgatg gcgcttgccg ccgttctccc
     4621 caacccccgc gaatggtccc ccgcccggcc aaacgcgatc gtgcaacgaa agatccggcg
     4681 cattcagcgg ctttcctcga acgcgccgtt ccccagcgac gaattgccgg acaaaaacga
     4741 atagatttcc tccatcactt ccgcgcagtc cggatcgcgc agcaggaggt ggtaagaccg
     4801 cggcaaaaag cggaggcggc agcccggctg ggaaaccgaa ttaaaccagc tcgtcagttg
     4861 atccggcgag atgtagggat cattccgccc gcccagcacc agcagcggcc gggggatgcg
     4921 gggacccgcc cggaggcagc cctccatcaa ccgggccatg cccgcgtaaa aaccgaggca
     4981 aacctggggc aggcggaagg gcgccttggc cagcgcctgt tgatactcgg tattcgcggt
     5041 gagcttcggc gcccgggcct ttccgtggat aaaaagcccc ggatgcagcg acgcccgggg
     5101 aatcacccgc gccagccacc ggatcaactc gacgatcacc gccggcgttt tctggcgcaa
     5161 ctccaccacc gggcaaagca gcaccgcccc gcgaaaccgc gcctggagct tcggagaatc
     5221 cgccagcgcg gcgatcgcca gcagcgcccc caggctttcc ccgctgacaa acaatgggca
     5281 aaccgccgga gtttgatcga ggaacgcctc caaatccccc aggatccgga cgacgtccac
     5341 ctcgcttccc cgccggcgga tttccggatc cagcccctgc ccccgcaacg ccggcgcgaa
     5401 gagcgatacc cccgccgcgg aaagaaattc ccccagcggc gcgaactccc gcggcgctga
     5461 tcccatgcca tgcagaaaat acacccgcgc cttttcctcc ttcgcccgcc aggcggcata
     5521 gggaaattcc gccccgtcgg aatggtaggt ttcctccacc ggcatgcaat gagaggacgc
     5581 ccgcgcgggc gcttgtgtca agccccggca aacgcttaac tcagcccatg aagaaattcc
     5641 ttttcatcct gctggccctc accgcttcca ccttcgccgg gcaactcgat ctcgccgtgg
     5701 tgcaatacgc caccgccaag gactccgcgg aactcgccgc cggattcgcc aacgtcgatc
     5761 tcgccgaggc gaccaatgcc gacagcgtca tcaagggaga caacgccatt cgcggcggct
     5821 ccgtgatctt cgcccagacg atccccgcct cccccggcag ccgcctgacc acctccaccc
     5881 ggctcgacgc ccgccgcgtg gacgtcacca ccgccctcaa cggcctgcat cttacggcga
     5941 acatctccat tatcaccggc gtgcagcagg cgcttcgcag ctacaccgag cgcacttatg
     6001 ccgccgaagg cgatctcaat g
//
LOCUS       DATDAY010000245         2411 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_1574967, whole genome shotgun sequence.
ACCESSION   DATDAY010000245 DATDAY010000000
VERSION     DATDAY010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2411)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2411)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2411
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1574967"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            11..307
                     /locus_tag="VIS74_04905"
     CDS             11..307
                     /locus_tag="VIS74_04905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792614.1"
                     /translation="MIKSLERFESAAALASSKQPPEDPREIEGYLANFQSMKLVVPRG
                     GGFVFAPASEELRLAVDALEAAYNQRPVTLIRTIYALADHKIQSFADSFKIKES"
     gene            311..577
                     /locus_tag="VIS74_04910"
     CDS             311..577
                     /locus_tag="VIS74_04910"
                     /inference="COORDINATES: protein motif:HMM:NF039620.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5985 family protein"
                     /protein_id="HEY5792615.1"
                     /translation="MEVFKFVLSLAAIAASATCTVLLFRGYCRKRVRLLMWSAFCFAG
                     LTLNNLFLFCDLVVFPDIDLRPIRLLAALTGMLFLLYGFIWDTE"
     gene            594..860
                     /locus_tag="VIS74_04915"
     CDS             594..860
                     /locus_tag="VIS74_04915"
                     /inference="COORDINATES: protein motif:HMM:NF039620.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5985 family protein"
                     /protein_id="HEY5792616.1"
                     /translation="MIVFLSGALTATYLIAAVFFLKFWRKTRDRLFSLFALAFTFFAL
                     NQVAAVFLDLSTERGSFAYLLRITGFVLILFAIIEKNRTPKKNG"
     gene            986..1336
                     /locus_tag="VIS74_04920"
     CDS             986..1336
                     /locus_tag="VIS74_04920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792617.1"
                     /translation="MLKSYLPAAIDDFSHISLQLFRALDFLPVVLVEALGTLGLRLLL
                     SFFSFHKGGSRPGGALVFNPMKKCGMDPIPPGRRNTDAPREGISVLANWLPPPLSVGA
                     AAVFLVVPDAAGFP"
     gene            1375..2070
                     /locus_tag="VIS74_04925"
     CDS             1375..2070
                     /locus_tag="VIS74_04925"
                     /inference="COORDINATES: protein motif:HMM:NF014373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HEY5792618.1"
                     /translation="MNTEISEFDVLIRLAVAIVAGALIGVNRQEHGRPAGLRTTMLVC
                     LAATGAMILANLLVATRGRPWDSYISMDVMRLPLGILTGVGFIGAGAMMHKGNFVLGV
                     TTAATLWFTSVMGLCFGSGEFTLGLLLLVASLGILWGLRWIEKNTKHRYDANVTVVVA
                     SEGPAESAIVGTIKEAGYQVSPLLAAVRETGREYRFEVSQHFRHDPSGPPEPLTRLVS
                     ENGIREIIWAKQQ"
     gene            2179..2394
                     /locus_tag="VIS74_04930"
     CDS             2179..2394
                     /locus_tag="VIS74_04930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792619.1"
                     /translation="MKKSAEPKRYFQDSWRPEDKAMESAEERQLEVDYPMQIARQRAE
                     RHKATPPLRGFEQAPRVSEPFEPSDEE"
BASE COUNT          448 a          691 c          705 g          567 t
ORIGIN      
        1 gttgcttctt ttgatcaagt ccctggaacg ttttgaatcg gcggcggcgt tggcgtcgag
       61 caaacagcct ccggaagatc cgcgcgaaat tgagggatac ctggcaaatt tccagtcgat
      121 gaaactggtc gtcccccgcg gcggtggctt cgtattcgcg ccggcctcgg aggaattgcg
      181 gctggcggtg gatgcgctgg aggccgctta caaccaacgt cctgtcacgc tcatccggac
      241 gatttatgct cttgccgatc acaagattca gtcgtttgct gattctttca aaatcaagga
      301 atcctaagcc atggaggtct tcaagtttgt gctttctctc gccgcgatcg cggccagtgc
      361 gacctgcacg gtcctcctgt tccggggcta ctgccggaag cgtgtgcggc ttctgatgtg
      421 gagcgccttt tgttttgcgg gactgaccct gaataatctc ttcctctttt gcgatttggt
      481 ggtgtttccc gacatcgatt tgcggccgat tcgtctgctg gcggccctca caggaatgct
      541 ttttctcctc tacggattta tctgggacac ggaatgaaga aggaccccgc accatgattg
      601 tgtttctttc cggagccctt acggccacct atctgatcgc agcggttttt ttcctgaaat
      661 tctggcgcaa gacccgcgac cggctttttt ccttgtttgc tctggccttc accttcttcg
      721 ccctgaatca ggtggccgcc gtctttctgg atctctccac cgaacggggc agttttgcgt
      781 atttgttgag gataaccggg ttcgtcctca tcctttttgc catcatcgaa aagaaccgga
      841 ctcccaaaaa aaacgggtga tcgtcgccca aggcgtggac gcgttacgcc cctcgcgagc
      901 gcccgctatc gggtaaaccg aagcgcttgg aaaagagggc gaatgcggtt ctgcgcaacc
      961 cgtcagagag gtcgcttgtt gcaccgtgct aaaatcctat ctgccggccg ctatcgacga
     1021 ttttagccac atttccctcc agctttttcg ggcgctcgat ttcctgcccg tggtccttgt
     1081 cgaggctctg gggacgctcg ggctccgtct gctcctgtct tttttctctt ttcacaaagg
     1141 gggatcgcgc cccggcgggg ctttggtttt caatccgatg aaaaaatgcg ggatggatcc
     1201 gatcccgccc ggcaggcgaa acaccgacgc tccgcgagag gggatctcag tattagccaa
     1261 ttggcttccg ccgccgctct cggtcggagc cgctgccgtt ttcctggtcg ttcctgatgc
     1321 ggcggggttc ccttgacacg ccgggcctga ggcggacaat ggatgcaggc caccatgaat
     1381 accgaaattt ccgaattcga tgtgcttatc cggctggccg ttgcgatcgt ggctggcgcg
     1441 ctgatcgggg tcaatcgcca ggaacacggc cgcccggccg gattgcgaac caccatgttg
     1501 gtctgcctgg ccgccacagg ggcgatgatt ctggcaaatc ttctggtcgc gacccgtggc
     1561 cgcccttggg actcttacat ttccatggac gtgatgcggc tgccgttggg aatccttacg
     1621 ggcgtcggat tcatcggcgc cggcgccatg atgcataagg gaaattttgt cctgggtgtc
     1681 accacggccg caacgctctg gttcacgtcg gtcatgggcc tctgcttcgg atccggcgag
     1741 ttcacgctgg ggctgcttct tcttgtggca agtctgggta tcctctgggg gctgcgctgg
     1801 attgaaaaaa ataccaaaca ccgttacgac gcgaacgtga cggtcgtggt ggcttccgaa
     1861 ggtcctgcgg agtctgcgat tgtcggcacc atcaaggagg ccggatacca ggtttccccg
     1921 ctccttgcgg cggtgaggga aaccggccgc gaataccggt tcgaggtttc gcaacatttt
     1981 cgccacgacc catccggtcc gccggaacca ttgacccggc tggtgagcga aaatgggatc
     2041 cgggaaatca tttgggccaa gcagcagtaa tttttgtcct gaaaaccgat agggcggaag
     2101 ttttatacgt ccgtcggatc gcaggggaac gactagatca taggaggtgg tgcatcaacg
     2161 ctccccctag agaaaccgat gaaaaaatcc gcggaaccca aacggtattt tcaggacagt
     2221 tggcggccgg aagacaaggc gatggaatcg gccgaggaac gtcagctgga agtggactat
     2281 cccatgcaga tcgcccggca gcgcgccgag cggcataagg ccacaccacc cctccgcggc
     2341 ttcgagcagg ctccccgcgt tagcgagcca ttcgagccat cagatgaaga atgaatggag
     2401 ggacgacctc c
//
LOCUS       DATDAY010000246         4427 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_2327420, whole genome shotgun sequence.
ACCESSION   DATDAY010000246 DATDAY010000000
VERSION     DATDAY010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 4427)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4427)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4427
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2327420"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(25..756)
                     /locus_tag="VIS74_04935"
     CDS             complement(25..756)
                     /locus_tag="VIS74_04935"
                     /inference="COORDINATES: protein motif:HMM:NF009044.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEY5792620.1"
                     /translation="MGRQWLQAKREVANLKKGQVVGKLVKEIMVAAKMGGPDPDGNAR
                     LFTAVEKARRASVTRDVIERAIKKGAGVGDDKLVMEHVLFEGYAPHKVPVIVEVYTDN
                     NNRTASDIRLLFKKAQLGAPGSNKFLFENVGLVEASHPDSSTDLEVAAIEAGANEFEP
                     LTHAQNDDIPADHLGARFITDRTATGAVSKWLKDNGWNVVTSELGYIAKSFPELTEEQ
                     LAEVGEFLQSVDDHDDVHRVWAAVK"
     gene            861..2180
                     /locus_tag="VIS74_04940"
     CDS             861..2180
                     /locus_tag="VIS74_04940"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEY5792621.1"
                     /translation="MKNILPTLLASALGLTVAAEGAVPAIELKVKPEREVLVENGPRE
                     TVVQVEIRAKPSDKKRTTPINLALVLDRSGSMAGAKIEKARQAAAVAVDQLDTDDYFS
                     LVVYDDEVEVLIPPQKVKDKEAVKRQIYAIRPGGSTALYAGVEKGAGQVRKYLDPEKV
                     NRLILLSDGLANVGPSSPGDLARLGKELRGEGLSVSTIGLGEDYNEDLMTALAEASHA
                     NYYYVQDAEKLPEIFTQELGTVKSVAGRNVQVIITLPEGVKPIRIIGEDEFAFKGQTA
                     TLPLSEFSAAQTRRFLISCEAPKVKDAEIALAQVELNYVDAESRQAVREIEKVTVRQS
                     PDEAASQDSIQAEVLANRAITENRLAKERAVKLADAGKAEEAARVLETQHTINASLPA
                     AAQSERLQQEQSELKAKSSELQNNGSLSKISRKAIQYDNYRDKTQKR"
     gene            2297..4015
                     /locus_tag="VIS74_04945"
     CDS             2297..4015
                     /locus_tag="VIS74_04945"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEY5792622.1"
                     /translation="MAIFLPSLGLGWLALRTAGEQRVLLERQEAGLRQAETDAISARI
                     REQMDQMQQRFAETVRSLARVRGAAALAEDFATKISGVWPEGGVPFAISPDGSLAFPS
                     AAQSRQDANFRDFVSNSGAFLSNQISERVFQTQSALPLPDKSTEKTRAKAESKEAPFL
                     SSFSVKRNVAPQRHAESQRQEAPASKSAPAVSDFQSATEGVTQGTLARFVQNELEVLF
                     WTRPDPGANWIFGVMLNAAQLAPLLQARLAGERDGSAQLAVLDERARPVARQPREFTA
                     DWKRPFVATEIGEALPQWEVALYLADPGKLTESARLVTTTLILLIALALAAILTGGFF
                     VTRDARQQLALAQKKTDFVSNVSHELKTPLTSIRLFAEMLAEGRAEDPEKRNRYLRIL
                     MSESERLTRLVNNVLDFARSEKGRRNYQRRAVDLRALIAGVWEGEQERLREKGFSAEW
                     QAEPGDYSAHCDPDAISQVLVNLLSNAEKYSNGEKEIGLGTGVRAGVLEIRVRDRGIG
                     IPRGLDQKIFEPFFRADDSLASGAQGSGLGLTLARRIARDHGGNLVARAREGGGSVFI
                     FTLPLT"
     gene            4012..>4427
                     /locus_tag="VIS74_04950"
     CDS             4012..>4427
                     /locus_tag="VIS74_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006983272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY5792623.1"
                     /translation="MSKRILIVEDDPHIRLGLADALGSEGYAITECRDGGEAIPLARQ
                     THPDLIILDVMLPRKSGYDVCRELREAKNRVPVLMLTAKGQEIDKVVGLELGADDYVT
                     KPFGVRELIARVHALLRRVQPEAPATAMPDQLVLGEV"
BASE COUNT          898 a         1370 c         1370 g          789 t
ORIGIN      
        1 gggatcagcg atcccggcta cagctcactt caccgcggcc cagacgcggt gcacgtcgtc
       61 gtggtcgtcc acggattgca gaaattcgcc gacttcggcc aattgctcct cggtaagttc
      121 gggaaaactc ttggcgatgt agccgagctc gctcgtcacc acgttccagc cattatcctt
      181 aagccatttg gaaacggctc cggtggcggt gcggtcggtg atgaaacgcg cgccgaggtg
      241 atccgcgggg atgtcatcat tctgcgcgtg cgtcagcggt tcgaattcat tcgcgcccgc
      301 ttcgatggcg gccacttcca ggtcggttga ggaatccgga tgcgaggcct cgaccaagcc
      361 gacgttttca aaaagaaatt tattgctgcc gggcgcgccg agctgcgcct ttttaaagag
      421 caggcggatg tcggaggcgg tgcgattgtt attgtcggtg taaacttcga cgatgaccgg
      481 caccttgtgc ggcgcgtagc cctcgaacag cacgtgctcc atcaccagct tgtcatcgcc
      541 aacccccgcg ccttttttga tcgcccgctc aatgacgtcc cgggtcacgg atgccctgcg
      601 cgccttttca accgcggtga acagccgggc gttgccgtcg ggatcggggc cgcccatctt
      661 ggcggcgacc atgatttcct tcaccagctt gcccacgacc tgacctttct tcagattggc
      721 aacctcccgt tttgcttgca gccattggcg tcccatagga ccggtgattt aagcaggatt
      781 ccgccgcttt tcacaatttt tttacaaact ccttacgcga ttctgacaac ccgccccgga
      841 aatcggcgca gggtaagggc gtgaaaaata tccttcctac cctattggct tcggcgctcg
      901 gcttgaccgt ggcggccgaa ggagccgttc ccgccatcga actcaaagtc aaacccgaac
      961 gcgaggtgct ggtggaaaac ggcccgcggg aaaccgtggt gcaggtcgag atccgcgcca
     1021 agccatcgga caaaaaacga acgactccga tcaatctcgc cctcgtgctg gatcgttcgg
     1081 gatcaatggc gggcgccaag atcgaaaaag cgcggcaggc ggcggcggtc gcggtggacc
     1141 agctcgacac ggacgattat ttttcgctgg tcgtgtatga cgacgaggtg gaggttctca
     1201 tcccgccgca aaaggtgaag gacaaggagg cggtcaagcg gcagatttac gccattcgtc
     1261 ccggcggcag cacggcgctg tatgccggag tggaaaaggg cgcggggcag gttcgcaaat
     1321 acctcgaccc cgaaaaggtc aaccgcctca tcctgctttc cgacggcctg gccaatgtcg
     1381 gcccctcctc gcccggggat ctcgcccggc tgggcaagga gctgcgcggc gaaggcctga
     1441 gcgtttcgac catcggcctt ggcgaggact acaacgaaga tctcatgacc gccctcgcgg
     1501 aagccagcca cgcgaattat tattacgtgc aggacgccga aaagctgccg gagattttca
     1561 cccaggagct cggcaccgtg aagagcgtgg cgggccgcaa cgtgcaggtc atcattacgc
     1621 tgccggaagg cgtgaagccg atccggatca tcggagaaga cgaatttgcg ttcaagggcc
     1681 agacggcaac ccttccgctt tcggaatttt ccgccgccca aacgcgccgg tttctcattt
     1741 cgtgcgaggc gccgaaggtg aaggatgcgg aaatcgcgtt ggcgcaggtg gagctgaatt
     1801 acgtggatgc ggagagccgc caggcggtgc gtgaaatcga aaaagtgacg gttcggcaaa
     1861 gcccggatga agccgcgtcg caggattcga tccaggccga agtgctcgca aatcgcgcca
     1921 tcaccgaaaa tcgcctggcc aaggaacggg cggtcaaact cgcggatgcg ggcaaggcgg
     1981 aggaggccgc gcgggtgctc gagacccaac acaccattaa tgccagcctg cccgccgcgg
     2041 cccaaagcga acggctgcaa caggagcagt ccgaattgaa ggcaaaaagc agcgaactgc
     2101 aaaacaatgg ctcgctgagc aaaatcagcc gcaaagccat ccaatacgac aactaccgcg
     2161 ataagacgca gaaacgttga aaaatcaatc gccaccgggg cgccgaacaa aggcttaacc
     2221 taaaaatctc tgtgtccccg gtgttctctg tggttaaaat tcctcggtcg tgccgcgtta
     2281 cgcgttgatt ctggtaatgg ccatctttct gcccagcctc gggctgggct ggctggcgct
     2341 gcgcacggcc ggggaacagc gggtgctcct cgaacggcag gaagccgggt tgcgccaggc
     2401 ggaaacggac gcgatctccg cgcggatccg cgagcaaatg gatcaaatgc aacaacggtt
     2461 cgccgagacc gtgcggagcc tggcccgagt gcggggcgcc gccgcgctgg ccgaggattt
     2521 tgccacgaaa atcagcgggg tctggccgga aggcggggtg ccatttgcga tttcaccgga
     2581 cgggtcgctg gccttcccct ccgccgccca gtcccgccag gacgcaaact tccgcgactt
     2641 tgtgtccaat agcggcgcct ttctcagcaa tcaaatttcc gagcgggttt ttcaaaccca
     2701 atcggcgttg cctctgccgg acaaatccac cgagaaaacc cgggcaaagg cggaatccaa
     2761 ggaggcgccg ttcctctctt cattcagcgt caaacgcaac gtcgcgccgc agcgtcatgc
     2821 cgaatctcag cggcaggaag cacccgcgtc gaaaagcgcg ccagccgttt ccgacttcca
     2881 aagcgccacg gaaggcgtca cgcaaggcac cctcgcgcgc tttgtgcaga acgaactcga
     2941 agtccttttc tggacccggc ccgatccggg cgcgaattgg atttttggcg tgatgctgaa
     3001 cgccgcccag ctcgcccccc tgctgcaagc ccgcctggcc ggagagcgcg acggcagcgc
     3061 gcaactcgcc gtgctcgacg aacgcgcccg gcccgtcgcg cggcaaccgc gcgagttcac
     3121 cgccgattgg aagcggccgt tcgtcgccac cgaaatcggc gaggcgctgc cgcaatggga
     3181 ggtcgcgctg tatctcgcgg accccggaaa gctcaccgaa tccgcgcggc tggtgaccac
     3241 gaccctgatt ctgctcattg ccctcgcgct ggccgccatt ctaaccggcg gctttttcgt
     3301 cacgcgcgac gcacggcaac aactggccct ggcgcagaaa aaaaccgatt tcgtttccaa
     3361 tgtctcgcac gagctgaaaa ctcccctcac ctcgatccgc ctcttcgcgg aaatgctggc
     3421 cgaaggacgc gcggaggatc cggaaaagcg gaaccgctac ctgcgcatcc tcatgagcga
     3481 aagcgagcgg ctgacgcggc tggtcaacaa tgtgctcgac tttgcccgat cggaaaaagg
     3541 ccgccggaac taccaacgcc gggccgtgga cttgcgggca ttgattgccg gcgtgtggga
     3601 aggcgaacag gagcggctgc gcgaaaaggg attcagcgcg gagtggcagg cggaacccgg
     3661 cgattattcc gctcattgcg atccggacgc gatctcgcag gtgctcgtta acctgctctc
     3721 gaatgccgag aaatacagca atggcgaaaa ggaaatcggg ctgggcaccg gggtccgcgc
     3781 gggtgtcctg gaaatccgtg tccgggatcg cggcatcggc atcccgcgcg ggctcgatca
     3841 aaaaattttc gagccattct ttcgcgccga cgattcgctg gcgagcggcg cgcaggggtc
     3901 cggcctcggc ttgacgctcg cccgccgcat cgcccgggat cacggcggca acctcgttgc
     3961 cagagcccgc gagggcggcg gcagtgtttt tatttttacc ctcccactga catgagcaaa
     4021 cgcattctga tcgtcgagga cgatccgcac attcgactcg ggctggcgga cgcgctcggc
     4081 agcgaaggtt acgccatcac cgaatgccgc gacggcggcg aggcgattcc gctcgcgcgg
     4141 cagacgcatc cagacttgat catcctcgac gtgatgctgc cgcgcaagag cggctacgat
     4201 gtctgccgcg aattgcgcga agcgaaaaac cgcgtgcccg tcctcatgct cacggccaag
     4261 ggacaggaaa tcgacaaggt cgtcgggttg gaactcggcg cggatgatta tgtcaccaag
     4321 ccctttggcg tgcgggaatt gatcgcgcgc gtgcatgcgc tgcttcggcg ggtccagccg
     4381 gaggctccgg cgacggcgat gccggatcaa ttggttttgg gggaagt
//
LOCUS       DATDAY010000247         2907 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_1775334, whole genome shotgun sequence.
ACCESSION   DATDAY010000247 DATDAY010000000
VERSION     DATDAY010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2907)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2907)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2907
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1775334"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..443)
                     /locus_tag="VIS74_04955"
     CDS             complement(<1..443)
                     /locus_tag="VIS74_04955"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY5792624.1"
                     /translation="MSSTLQLPSYQDQSPEVAARFAKLKSRPVILETRKLTKTFHNHT
                     PLNEISLQVFRRELLCVVGPSGCGKSTFIRIIAGLETPTSGEVAVEGRPVSGPGSDRG
                     MVFQGYTLFPWLTVKRNVMFGLQVSGKSAFEAEGEAREWINLVGLS"
     gene            complement(440..1471)
                     /locus_tag="VIS74_04960"
     CDS             complement(440..1471)
                     /locus_tag="VIS74_04960"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY5792625.1"
                     /translation="MAEASKNKWLAPFGQLSPSRQLLLVWVSFMLPIAVWAVISYVPF
                     IWHPLVKISDPGGVSYFSREGLIERTVFQRENARMAEAGKPVAAGTPANPIFLPAPHE
                     VAVAFYKAFTTPPKRKGEKWLHESLWQSIQVIFWGFTISSIIGVPLGILCGSFPFFSK
                     ITEPFVDFTRYMPAPAFGALAVAVLGIYDPPKIAIIVLGTFFQQVLVIANTTRKLDTS
                     LVEASQTLGASRRQILFRVIVPGISVDLYNDMRILLGWAWTYLIVAEYIGASSGITYF
                     INQQAKYRIYDNVFAAIIMIGILGFTTDRILDWIGNQIFPWQKTTKPGLIRTIALARQ
                     RKKQETVTA"
     gene            complement(1504..2484)
                     /locus_tag="VIS74_04965"
     CDS             complement(1504..2484)
                     /locus_tag="VIS74_04965"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY5792626.1"
                     /translation="MKFTNSAFKWLAGWAAALILAGAAQAEPLKIGYSDWPGWVAWEV
                     AIQKDWFKEAGVEVNFEWFEYVPSMEAFAAGKIDAVCMTNGDALVTGATGAPSKAILI
                     NDYSNGNDMIVGQPDIQSIKDLKGKKIGVEIGFVDHLLLLKGLAANGLAEADVELVNV
                     PTSETPKALASGDVSAIAAWQPNSGQALKAVPGSSAIYTSADVPGLIYDVLAVNPASL
                     SARPEDWKKVLQVWYKVVAYLNDPATQADAIKLMAARVNLSPEEYKPFLTGTKILTLE
                     EAKKAFEKGDSLGSIYGSNVVVDQFNVDNKVYEKPQPAADYLDPSLTGGL"
     gene            2696..>2907
                     /locus_tag="VIS74_04970"
     CDS             2696..>2907
                     /locus_tag="VIS74_04970"
                     /inference="COORDINATES: protein motif:HMM:NF014471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HEY5792627.1"
                     /translation="MMAASDSPRPSFGEALRFWLKLGFISFGGPAGQIAILHAELVEK
                     RKWISEERFLHALNFCMLLPGPEAQQL"
BASE COUNT          559 a          860 c          890 g          598 t
ORIGIN      
        1 gacagcccga ccagattgat ccactcgcgg gcttccccct cggcttcgaa cgcggatttt
       61 ccgctcactt gaaggccgaa catcacgttc cttttgaccg tgagccacgg gaaaagcgtg
      121 tagccctgga agaccatgcc gcgatccgaa cccggaccgc tgacgggccg gccttcgacc
      181 gcgacttcgc cgctcgtcgg cgtttcgagg cccgcgatga tgcggatgaa ggtggacttg
      241 ccgcagcccg aggggcccac cacgcacagg agttcccgcc ggaagacctg cagggaaatc
      301 tcattgagcg gggtgtgatt gtgaaaggtt ttcgtgagtt ttcgggtctc caggatcacc
      361 ggccgcgatt tgagcttggc aaaccgcgcg gcgacctccg gcgactggtc ttgatagctg
      421 ggaagttgaa gggtgctgct catgccgtga cggtctcctg ttttttgcgc tggcgggcca
      481 gggcgatggt ccggatcaat ccgggtttcg tggttttctg ccaggggaaa atttggttgc
      541 cgatccagtc gaggatccgg tcggtggtga agccgaggat gccgatcatg atgatggccg
      601 caaagacgtt gtcgtagatt cgatacttcg cctgctggtt gatgaagtag gtgatgcccg
      661 aactcgcgcc gatgtattcc gcgacgatga ggtaggtcca cgcccagccg agcaggatgc
      721 gcatgtcatt gtagagatcc acgctgatgc ccggcacgat gacgcggaac aaaatctgcc
      781 gccggctggc gcccagcgtt tgcgaggctt cgacgagcga ggtgtcgagc ttgcgcgtgg
      841 tattggcgat caccagcacc tgctggaaaa acgtgccgag gacaatgatc gcgatcttcg
      901 gcggatcgta aatgccgaga acggccaccg ccagcgcgcc aaacgccggc gcgggcatgt
      961 agcgggtgaa gtccacgaaa ggttccgtga tcttggaaaa aaacggaaat gaaccgcaaa
     1021 gaatgcccag cggcacgccg atgatcgagc tgatggtaaa tccccaaaaa atcacctgga
     1081 tgctttgcca caggctttcg tggagccatt tttcgccctt gcgcttcggc ggcgtggtga
     1141 aggccttgta aaaggcgacg gccacctcgt gcggcgcggg caggaagatg ggattcgccg
     1201 gcgtgccggc ggccacgggt tttcccgcct ccgccattcg cgcgttttcc cgctggaaaa
     1261 ccgtgcgttc aatcaggccc tccctggaaa aataggacac cccgcccggg tcgctgatct
     1321 tgaccagcgg gtgccagatg aacggcacgt agctgatgac cgcccagacg gcgatcggca
     1381 gcatgaacga aacccacacc aggagcaatt gccgggaggg agacaattgc ccgaagggcg
     1441 ccagccattt gtttttggat gcttccgcca tatgcctccc ctttttggaa tcgcgttcgc
     1501 gaatcagagt ccgccggtca gcgagggatc caggtaatcc gccgccggct gcggtttttc
     1561 gtaaaccttg ttgtccacgt tgaattgatc caccaccacg ttcgagccgt agatggaacc
     1621 gagcgaatca cctttctcaa aggccttctt cgcctcctcg agcgtcagga ttttcgttcc
     1681 cgtgaggaag ggcttgtatt cctccgggct gagattgacg cgggcggcca tgagcttgat
     1741 ggcatccgcc tgcgtggcgg gatcgttcag gtaagccacc accttatacc agacctgcag
     1801 cacttttttc caatcctcgg gccgcgcgga aaggctggcg ggattcaccg ccagcacgtc
     1861 gtagatgagg ccgggcacgt cggcgctcgt gtagatggcc gaggagccgg gaaccgcctt
     1921 caacgcctgc ccggaattcg gctgccaggc ggcaatggcg gacacgtccc ccgaagcgag
     1981 cgccttgggc gtttccgagg tcggcacgtt cacgagctcg acatcggctt ccgccagtcc
     2041 attcgccgcc agccccttga ggagcaggag atggtccacg aagccgattt ccacgccgat
     2101 tttcttgccc ttgagatcct tgatcgactg gatgtccggc tggccgacga tcatgtcatt
     2161 gccgttggaa taatcgttga tgagaatcgc cttgctcggc gcgcccgtgg cgccggtgac
     2221 cagcgcgtcg ccattggtca tgcagaccgc gtcaattttt ccggcggcaa aggcctccat
     2281 cgagggcacg tattcgaacc attcgaaatt cacctccacc cccgcttcct tgaaccagtc
     2341 tttttgaatg gccacctccc aggccaccca gcccggccag tcgctgtagc cgattttaag
     2401 cggttccgcc tgggcggcgc cggcgaggat caatgcggcc gcccagccag cgagccattt
     2461 gaatgcggag ttcgtgaatt tcatgagtat tacgagttga tagttgggta atacgaatta
     2521 aaagcatttc ccatgccagg ttggtccgcg ggaaacgcaa ccccgcgggc aaatccgcat
     2581 tcaacgcatt tcgttcccgc gaatttttga gttcctttgc ggctaaagat cgccttcgcg
     2641 cgaggcgggg aaagccgggc ttgtgtcccg gcccggagcc atgttttctg agcgaatgat
     2701 ggccgcttcc gattctccgc gtccgagctt tggagaagcc ctccgcttct ggctgaagct
     2761 tggattcatc agcttcggcg ggccggcggg acagatcgcc atcctgcacg cggagctggt
     2821 ggaaaaacgc aaatggatca gcgaggaacg gtttctccac gcgctgaatt tctgcatgct
     2881 gctcccggga ccggaggccc agcagct
//
LOCUS       DATDAY010000248         3847 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_7768, whole genome shotgun sequence.
ACCESSION   DATDAY010000248 DATDAY010000000
VERSION     DATDAY010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 3847)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3847)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3847
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7768"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..374)
                     /locus_tag="VIS74_04975"
     CDS             complement(<1..374)
                     /locus_tag="VIS74_04975"
                     /inference="COORDINATES: protein motif:HMM:NF019500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel"
                     /protein_id="HEY5792628.1"
                     /translation="MSDFPKRSLYSVYLMGAGLATVLFAVHTFILFETAHAIFRLAEP
                     FRETLGLRVAGPLLVLGCFLMWFVTHLLEAVVWAVFFWRKRLVRSFAEGLYFTGTSLT
                     ALGYGDVVLTPPWRGLGPIMAT"
     gene            complement(434..1828)
                     /locus_tag="VIS74_04980"
     CDS             complement(434..1828)
                     /locus_tag="VIS74_04980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEY5792629.1"
                     /translation="MTPNLVNILLVDRTGRFAPALQRTLLAAEYRVAGVSTEQAARQR
                     MAAEPFNIVIKSFETAESDGVPLMEKLRELSPDTEFILVSEGGSIRTAIDAIHHGAFD
                     HLSWPVDNEHILRSVRQALEHQALVAADPGIRGRLRRKKEVDIFAGSNPLMNEVQQIL
                     RRVAPSDVTVLIEGESGTGKEIAARALHEKSPRREGPFVAVNCAALPDTLIESEFFGH
                     VRGAFTGAIADKPGRFELARGGTLFLDEIGDLSPLGQADLLRVLEDGVFRPIGSRTVQ
                     RANVRIVAATNRNLEERCAEGRFREDLFYRLNVITLVLPPLRERAEDIPHLLETFSRH
                     FAARHKRPPKRFSPAAVRLLCACGWPGNVRQLRNLVERLALTAPAHRIEPEHLPAKIR
                     GERPANGLAAVSVGMTLAEAEAELIRQTLVRAGNRRTEAARMLGISRRTLHYKLGRLG
                     GGVNPEPPRELSEK"
     gene            2011..2412
                     /locus_tag="VIS74_04985"
     CDS             2011..2412
                     /locus_tag="VIS74_04985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HEY5792630.1"
                     /translation="MPTHSSIVQALKRLDQLEQGRSPLEKALTKTELRLAIETLRWNL
                     PAAVLNRHDRLLQRGLPSVARVEHGTCSACHRKLPSGHLNRLLHDSGLEICDYCGAFL
                     CLEAAPADSPAPRRHRLAPRRPPRKKIARTA"
     gene            2409..>3847
                     /locus_tag="VIS74_04990"
     CDS             2409..>3847
                     /locus_tag="VIS74_04990"
                     /inference="COORDINATES: protein motif:HMM:NF013109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HEY5792631.1"
                     /translation="MSRTSRLFHPRILGLFSGNYSRERLAADVLSGVTVGLIALPLSL
                     ALGIASIPAGTATPFPPPALGLFTAIIAGFLISFLGGSRVQIGGPTAAFIPIVLMIVE
                     EHGYGGLVLATMMAGVILILMGLTRMGALIKFIPWPVTSGFTTGIAVSIMASQAADFL
                     GIHASTGPPREFIEKFPWLAANLVHLNPASLLLAAACVLGIVVWPRLGIRRIPGSIVV
                     MLGAAGVVAVLGWHETLGIATIGSKFGPQAIPGHLPPPLWPELDWNTVRSLIGPATTI
                     ALLGAIESLLSAVVADGLANDRHSSNTELVAQGIANVVCPFFGGLPATGAIARTSANV
                     SNGGTSPVAGIVHALTLLAIVLLFSQWAALVPLAAMSAVLLMVAIRMGEWHELTRLNR
                     MPRSDALVLLTTFALTVLFDLVIAVEIGMVLAAILFIKRVSETTEISRVTPDDVLESP
                     EQLAVGKSIPEGVRVYRIFGPFLFGAAEK"
BASE COUNT          663 a         1302 c         1173 g          709 t
ORIGIN      
        1 ttcgtggcca tgatcgggcc aagcccgcgc cagggcgggg tcagcaccac atcgccataa
       61 ccgagcgcgg tcagcgaggt gccggtgaaa tacagccctt cggcaaagct gcgcaccagc
      121 cgcttgcgcc agaaaaatac cgcccacacc acggcttcca gcaggtgcgt gacgaaccac
      181 attaaaaagc agccgagaac cagcagcggc ccggccacgc gcaggcccaa ggtttcccgg
      241 aacggctcgg ccagccggaa aatggcgtgc gccgtttcaa acaggatgaa ggtgtgaacc
      301 gcaaacagga ccgtcgccag cccggcgccc atcaaataca ccgaataaag ggaacgcttc
      361 gggaaatcgc tcatcaatgt ttaggggatg agccaagcaa ggttccccgg gagccgcccg
      421 ggccgcaaat ggattatttt tccgataatt cccttggtgg ttcagggttc actcccccgc
      481 ctagccggcc gagcttgtag tgcaatgtcc ggcggctgat gcccagcatc cgggcggcct
      541 ccgttcggcg gtttcccgcg cgcaccaggg tttggcggat caattccgcc tccgcctcgg
      601 ccagcgtcat tccgaccgag acggccgcca ggccattggc cggccgctct ccgcggattt
      661 tggccgggag gtgctccggc tcgatccggt gggctggcgc cgtcaacgcc aggcgctcga
      721 ccagattgcg caactgccgg acgtttcccg gccagccgca ggcgcagagc agccggacgg
      781 ccgccgggga aaatcgcttg ggcggccgct tgtgccgggc cgcaaaatgc cgggaaaacg
      841 tctccagcaa gtggggaata tcctccgcgc gttcccgtag cggcggcagg accagcgtga
      901 tgacattcag ccggtaaaac agatcctcgc ggaaccggcc ttccgcgcag cgctcctcca
      961 ggttgcgatt cgtcgcggcg acgatgcgca cattggcgcg ctgcaccgtg cggctgccga
     1021 tcggccggaa gaccccgtcc tccagcaccc gcaggaggtc cgcctggccc agcggcgaca
     1081 agtccccgat ttcatcgaga aacagggtgc cgccgcgcgc cagttcgaag cgcccgggtt
     1141 tgtcggcgat cgcccccgtg aaggcgccgc gcacgtggcc gaagaattcg gattcgatga
     1201 gcgtatccgg cagcgcggcg caattcaccg ccacaaaggg cccctcccgc cgcgggcttt
     1261 tttcgtgcag cgcccgcgcg gcgatttcct tgcccgtgcc gctttccccc tcgatcaata
     1321 ccgtcacgtc cgaaggcgcc acccgccgca ggatttgctg cacctcgttc atcaggggat
     1381 tgctgccggc aaagatatcc acctcctttt tccggcgcag ccgtccgcgg atgcccggat
     1441 ccgcagccac cagggcctga tgctccaagg cctgccgcac ggaacgcagg atgtgctcgt
     1501 tgtccaccgg ccaggacaga tggtcaaagg cgccgtggtg aatggcatcg atggccgtgc
     1561 gaatgctgcc tccctcgctg accaagataa attcggtgtc cggagaaagc tcgcgcagct
     1621 tttccatcaa gggcacgccg tcgctctccg cggtttcgaa ggatttgatg acaatgttaa
     1681 aaggctccgc cgccatccgc tgccgggccg cctgctcggt ggaaacgccc gccacccgat
     1741 attccgcggc cagcagtgtc cgctgcaggg ccggcgcgaa gcgcccggtt cggtccacga
     1801 gcagaatgtt gacgagattg ggagtcatgg gatgcgccgc cggagttggc aataattgcg
     1861 cgactttgct aaaaatagcg caattattgc gctattgtca cggcttaatc ggaatgggaa
     1921 aacgaccatc caaattttaa tgcgtttgat ccaaaattgt tacgagtaat ccaatacttc
     1981 tggcacgccg attgcgtaaa gatccctgcc atgcccaccc actcatccat tgtgcaagcg
     2041 ttgaaacggc tcgatcaact ggagcaaggc cgctctccgc tggagaaggc tttgaccaaa
     2101 accgaactcc ggctcgccat tgagaccctg cggtggaacc tgccggcggc ggtgctcaat
     2161 cgccacgacc gcctgcttca gcggggactc cccagcgtgg cccgggtgga acacggcacc
     2221 tgctccgcct gccaccggaa acttcccagc gggcatttga atcgcctgct ccacgactcc
     2281 ggcctggaga tctgcgatta ttgcggcgcc ttcctctgcc tggaagcggc gccagcggat
     2341 tccccggcgc cccgccgcca ccgcctcgcc ccgcgccgtc cgccccggaa aaaaatcgcc
     2401 cggactgcat gagccggacc tcccgtcttt ttcatccaag aatcctgggt cttttttcgg
     2461 ggaattattc ccgcgaaagg ctggcggcgg acgttctgtc cggcgtcacc gtgggcctca
     2521 tcgcgctgcc gctttccctg gcgctgggga tcgccagcat cccggcggga acggccacgc
     2581 cctttccgcc gcccgccctg gggctgttca cggccatcat tgccggattt ttgatctcgt
     2641 tcctcggggg cagccgggtg cagatcggcg gcccgacggc ggcgttcatt cccatcgtgc
     2701 tcatgattgt ggaagagcac ggttacggcg gcttggtcct ggcgaccatg atggcggggg
     2761 tcattctgat tctgatgggg ctgacgcgca tgggggcgct catcaaattc atcccctggc
     2821 ccgtcaccag cgggtttacc accggcatcg cggtttccat catggcctcc caggcggcgg
     2881 actttctcgg aattcatgca tccacgggtc cgccgcgcga attcatcgaa aaattcccct
     2941 ggctggctgc caatttggtt cacctcaatc ccgccagcct gctcctggcc gccgcctgtg
     3001 tgctgggaat cgtggtctgg ccccggctgg gcatccggcg cattccgggt tcgatcgtcg
     3061 tcatgttggg ggccgcgggg gtcgtggcgg tgctgggatg gcacgaaacg ctcggcatcg
     3121 ccaccatcgg ctccaaattc gggccgcagg ccatacccgg ccaccttccg ccgccgctct
     3181 ggccggagct cgattggaac acggtgcgca gcctcatcgg gccggccacc accatcgccc
     3241 tgctcggcgc aatcgaatcc ctgctctccg ccgtggtcgc ggacgggctc gccaatgacc
     3301 ggcacagcag caataccgaa ctggtcgccc agggaatcgc caatgtcgtc tgccccttct
     3361 tcggcggact gcccgcgacc ggggcgatcg cccgcacttc ggccaacgtc agcaacggcg
     3421 gcacctcccc ggttgccggc atcgtgcacg cgctcacgct gctcgccatt gtcctgcttt
     3481 tttcccaatg ggccgcgctc gtgccgctcg cggcgatgtc cgcggtattg ctcatggttg
     3541 cgatccggat gggcgaatgg cacgagctca cgcggctcaa ccgcatgccg cgcagcgacg
     3601 cgctcgtgct gctgaccacc ttcgcgctca ccgtgctctt cgacctcgtg atcgccgtgg
     3661 aaatcggcat ggtgctggcg gcgattttgt tcatcaagcg ggtttccgaa acgacggaga
     3721 tctcccgcgt caccccggac gatgtgctgg agtcccccga gcagctcgcg gtgggaaaga
     3781 gcattccgga gggcgtgcgg gtatatcgca ttttcggccc gtttttattc ggggcggcgg
     3841 aaaaaat
//
LOCUS       DATDAY010000249         3786 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_1129018, whole genome shotgun sequence.
ACCESSION   DATDAY010000249 DATDAY010000000
VERSION     DATDAY010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 3786)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3786)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3786
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1129018"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(63..1052)
                     /locus_tag="VIS74_04995"
     CDS             complement(63..1052)
                     /locus_tag="VIS74_04995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792632.1"
                     /translation="MQTWEYPQLAERIGAFIRDPAANDFGNLALAIHQMQARQNAPYG
                     KFCAGRAAPAAWWEIPAVPLDAFRHFSMIAFPPEEIRTTFRTSGTTGEGYGQHHFSSL
                     ALYETAALSGWRQAGLPEGNVRALLASEQEACHSSLSYMAAWLTNRFYLRGGEFQWDA
                     LERGLAGAGSPVCLFGTALGFLNWFDRLGDHRLELPPGSLAVETGGYKGADRSLPKDA
                     LYALFERHLGLAGDAVVNEYGMTELSSQFYTRGLGRPHRGGHWVRARVIDPATGGEAA
                     DGETGVLRIFDLANLGSALALQTRDLAVRRGADFELIGRDPQALPRGCSRAVR"
     gene            1137..>3786
                     /locus_tag="VIS74_05000"
     CDS             1137..>3786
                     /locus_tag="VIS74_05000"
                     /inference="COORDINATES: protein motif:HMM:NF024915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY5792633.1"
                     /translation="MTRAFYRQIRILLLAACLPALHAQTGPAADYTADELLLAARNAY
                     NAGKWPQATTLYEQFLSSFGQAEGIAELLPRIRYDLAFGYLQQRKYAEALEKIDLFLG
                     TPPPPAALQLQEMTFWKGVCEMQEEACDAARATLEKFIGFFPPTINPVLRQQNPSAGK
                     VEDAKLLVGSIYLLQEKYREAAEYLAAAKKTMAPETRGRATVLELYALLQSGEKARAL
                     DLVVAEFPRAGELTQLAAFQTLSLQLGSELLEAKEYRKAILCLQRIWSADRLLKHQEG
                     RLAALEERLQALEANPKSDPYQKFLATQMIAKIKREIENFRKIAHYDAALRLRLATAY
                     QAMKRYREAGLILEAMLREMPPSPVVEAASVNLVQCWFAIERWPKVVESAQAFAEKFP
                     QSKSRPLALYLAAIAEQKDLRYAESIALGDAILKNFPQSEFAPRALFMKGFTQLLAER
                     NPAAIQTFEEFQKAYPDHELAESALYWRGMGCSLDKQFEKCREVMDEYLGRYPAGAFV
                     AAAKFRQAYAAQQLMDFPRSIAELHACLRAYPEGENVSEALVLLGDAYMNQGEMELGI
                     AAFKRIPPADRRFFEEGWFKAGKALKLMEQPGELRRHMERFRGEHPRSPRVAEAIYWI
                     GWVYKQQDQPGKAREVYWSAIREWGNDPAIRSVDDLIPALAKLYKGSDEQAQLAAELS
                     DLAEEAKRRDEKTLAMRALWGEGYVLRKSNPGKASQRFQEAAALANVQTANPLLLADF
                     AEACQLAGQEKEAARMWRDLVKWNPRAPQKDRALCALGLDELRQGNAKAALDYFDRFE
                     RDTLGSSRLLGKVMLTRARLLEERGQPAKAAESLETLLANKTSSGLEKAEALYRIAEM
                     HMKNGKPGLAVPYYQRIYVMHGRWRDW"
BASE COUNT          728 a         1271 c         1189 g          598 t
ORIGIN      
        1 tgatccactc gatcatgatg ggccaccacc agacacgaac ggcggcatcc ggcgaagcaa
       61 aatcatcgca cggcccggga gcagccccgg ggcagggcct ggggatcgcg cccgatcaat
      121 tcaaaatccg ccccgcgccg cacggccaga tcgcgtgttt gcagggccag cgcggagccg
      181 agattggcga gatcgaaaat ccgcagcacg ccggtttccc catccgccgc ctcgccgccg
      241 gtggcgggat cgatcaccct cgcccggacc cagtgcccgc cgcggtgcgg gcggccgagg
      301 ccgcgggtgt agaactgcga gctgagctcc gtcatgccgt attcgttcac caccgcatcc
      361 ccagccagcc cgagatgccg ttcgaaaagc gcgtagagcg cgtccttcgg cagcgagcga
      421 tcggcgccct tgtaaccacc ggtttcgacg gccaggctgc cgggcggcaa ttcgaggcgg
      481 tggtcgccca gccggtcgaa ccaattcaaa aacccgagcg ccgtgccgaa gaggcagacc
      541 ggcgatccgg cgccggccag gccgcgttcc agggcgtccc attggaattc gccgccgcgc
      601 agataaaacc ggttcgtcag ccaggccgcc atatagctta gggacgaatg gcatgcttcc
      661 tgctcggaag ccagcagcgc gcgcacgttg ccctcgggca agcctgcctg acgccagccg
      721 gagagggccg ccgtctcata gagcgccaac gaactaaaat gatgctgccc atatccctcg
      781 cccgtcgttc cgctggtgcg gaaggtcgtc cggatttcct ccggcgggaa ggcgatcatg
      841 gaaaaatgcc ggaaggcgtc cagcggaacc gcgggaattt cccaccacgc cgccggcgcc
      901 gcccggcccg cgcagaattt tccatacggc gcgttttgcc gggcctgcat ctggtgaatg
      961 gccagcgcga gattgccgaa atcattggcc gccggatcgc ggatgaacgc gccgattcgt
     1021 tcggccagtt gcggatattc ccaagtttgc atcggaagtt acaacctttt cacaactccg
     1081 gcctgccgga aagccccggc cgccggttga ttcgctgaaa tgcccccatg cgccgtttga
     1141 cccgagcctt ttaccgacaa atccgcatcc tgttgctcgc ggcctgcctg cccgcgctgc
     1201 acgcccagac cggccccgcc gcggattaca cggcggacga attgctgctc gcggcccgga
     1261 acgcctacaa cgccgggaaa tggccccagg ccaccaccct ctacgagcag ttcctgtcca
     1321 gcttcggcca ggccgagggg atcgccgagc tgcttccgcg catccgctac gatctcgcct
     1381 tcggttacct gcagcagcgg aaatacgccg aagcgctgga aaaaattgat ctctttctcg
     1441 gcaccccgcc gccgcccgcc gccctgcaac tccaggaaat gaccttctgg aagggcgtct
     1501 gcgaaatgca ggaggaggcc tgcgacgccg cccgcgcgac gctcgaaaaa ttcatcggct
     1561 tctttcctcc caccatcaat cccgtgctcc gccagcagaa tccctccgcc ggcaaggtgg
     1621 aggacgcgaa gctgctcgtg ggctcgatct atctgcttca ggaaaaatac cgggaagccg
     1681 cggagtatct cgccgccgcg aagaagacga tggcgccgga aacccggggc cgcgcgacgg
     1741 tgctggaact ttacgcgctg ttgcaatccg gggaaaaggc ccgcgccctg gatctcgtgg
     1801 tggcggaatt tccccgcgcc ggcgagctca cccagctcgc ggccttccag acgctctccc
     1861 tgcagctcgg ctcggaactg ctggaggcga aggaataccg gaaggccatt ctctgcctgc
     1921 agcgcatctg gtccgccgac cgcctgctca agcatcagga gggccgcctg gccgcgctcg
     1981 aggagcggct ccaggcgctg gaagcgaacc cgaagagcga cccgtatcaa aaattcctcg
     2041 cgacccagat gattgcgaag atcaagcggg agatcgaaaa tttccggaag attgcccatt
     2101 acgatgcggc cctccgcctg cgcctggcca ccgcgtatca ggcgatgaag cgctaccgcg
     2161 aggccgggct catcctggaa gccatgctcc gcgaaatgcc gcccagcccg gtcgtcgagg
     2221 ccgcgtcggt gaatctggtc cagtgctggt tcgccatcga acgctggccg aaggtcgtcg
     2281 aaagcgccca ggcgttcgcg gagaaatttc cgcaatccaa aagccggccg ctcgccctct
     2341 acctggcggc gatcgccgag cagaaggatt tgcgctatgc ggaatccatc gcgctcggcg
     2401 acgcgatcct gaaaaatttt ccgcagtccg aattcgcgcc gcgcgccctg ttcatgaagg
     2461 gattcacgca gctcctcgcg gagcggaatc cggccgcgat tcagaccttc gaggaatttc
     2521 agaaagcgta tccggatcac gaactggccg aatccgccct ttactggcgg ggcatgggtt
     2581 gctccctcga caagcagttt gagaaatgcc gcgaggtcat ggacgaatac cttggccgtt
     2641 atcccgcggg cgccttcgtg gccgccgcga aattccgcca ggcctacgcc gcgcagcagc
     2701 tcatggattt cccccgcagc atcgccgaac tccacgcctg cctgcgcgct tatccggagg
     2761 gtgaaaacgt gagcgaggcg ctggtgctcc tgggcgacgc ctatatgaac caaggcgaaa
     2821 tggagctggg catcgccgcg ttcaagcgca tccctccggc ggaccggcgc ttcttcgagg
     2881 aaggctggtt caaggccggc aaggcgctca agttgatgga gcagcccggc gaactgcgcc
     2941 ggcacatgga gcgattccgc ggcgagcatc cgaggagccc gcgcgtggcg gaggcgattt
     3001 attggatcgg ctgggtttac aagcagcagg accagcccgg caaggcgcgc gaggtgtatt
     3061 ggagcgcgat ccgcgagtgg ggcaacgacc cggccattcg ctccgtggac gatctgatcc
     3121 ccgcgctcgc gaaactttac aagggctccg acgagcaggc ccaactcgcc gcggaacttt
     3181 ccgacctcgc ggaggaggcc aaacggaggg acgaaaagac cctcgcgatg cgcgccctct
     3241 ggggcgaagg ttacgtgctc cgaaaatcga atcccggcaa ggccagccag cgcttccagg
     3301 aagcggcggc gctggccaat gtgcagacgg ccaaccccct gctgctggcc gatttcgccg
     3361 aggcctgcca actcgcgggc caggaaaagg aagccgcgcg gatgtggagg gatctggtca
     3421 aatggaatcc gcgcgcgccg cagaaggacc gcgcgctctg cgcgctcggc ctggacgaat
     3481 tgcgccaggg aaacgcgaag gcggcgctgg attatttcga ccgcttcgag cgcgacaccc
     3541 tgggctcgtc gcggctgctc ggcaaggtca tgctgacccg cgcccggctg ctggaggagc
     3601 gcggccagcc ggcaaaggcg gcggagtccc tggaaaccct gctggcaaac aaaacctcca
     3661 gcggcctgga aaaagccgag gcgctttacc gcatcgccga aatgcacatg aagaacggca
     3721 agcccggcct ggccgttcct tattatcaac gcatctatgt catgcatggc cgctggcgcg
     3781 actggg
//
LOCUS       DATDAY010000250         2258 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_549134, whole genome shotgun sequence.
ACCESSION   DATDAY010000250 DATDAY010000000
VERSION     DATDAY010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2258)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2258)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2258
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_549134"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1081)
                     /locus_tag="VIS74_05005"
     CDS             complement(<1..1081)
                     /locus_tag="VIS74_05005"
                     /inference="COORDINATES: protein motif:HMM:NF015531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEY5792634.1"
                     /translation="MKLLPFAGALLLPSLTLLAQQPSPTPAPTPAATPSPKPAGTPAP
                     AADLVNQLNDTQLDQAVDLLKTQFLNPAALNDQELRRARLEGLLDRLGPGAHLYLESA
                     KPAAAAPGRFLAEVLDSQVGYIRLGKLDADTLTQLDAVLAGFKEKSVHAVVLDLRSAG
                     YNADYEQAAAFARRFCEKGRLLFRVRKPSAKQERIFTSNQDSVFTGKVVVLTDRQTAG
                     AGEILAAVLRRQARAMIVGTSTAGQAVEFSEVPLGGGVTLKIAVSEATVPDAEAIYPR
                     GVKPDILVALPADQQAEIFRQSAEKGVSQFVYERERPRMNEAALIANLNPEIDGSGGS
                     AKNFDNPALKDTVLQRAVDLLTALSF"
     gene            complement(1078..>2258)
                     /gene="purD"
                     /locus_tag="VIS74_05010"
     CDS             complement(1078..>2258)
                     /gene="purD"
                     /locus_tag="VIS74_05010"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEY5792635.1"
                     /translation="PGNAGTGEFATNVGIGAGEISALADFAEAEGIDLTVVGPDDTLA
                     AGVVDAFEKRGLAIFGPSASAARLESSKAFAKDFMRRHGIPTADYREFTAAAEARDYC
                     EQAAYPLVIKADGLALGKGVIIAQDRAEAAAAIDRLMEKREFGAAGDRVVVEEFLTGV
                     ECSIHALVDGESWLLFPDCRDHKQAFDGGIGPNTGGMGTISPSHSAGPELLRTVRAEV
                     LDRFVAGLKADGLDFRGMLFPGLMITPAGPKVLEFNCRFGDPETQVLMRRLQSDLLDL
                     LEATVRRRLRDARPEWDAREAVCVIQASGGYPGDYVKGREITGIAEAETLPEIKVFHA
                     GTKRDAGSHLVTSGGRVLGVTALGASLAEARARAYAGVEKIRFENGFCRTDIGLLSRP
                     "
BASE COUNT          369 a          788 c          724 g          377 t
ORIGIN      
        1 aaaagctcag cgcggtgagc agatccaccg cgcgctgcag caccgtgtcc ttgagcgcgg
       61 gattgtcgaa gtttttcgcc gagccgcccg agccgtcgat ctccggattc agattcgcga
      121 tcaacgccgc ctcattcatc cgcggacgtt cccgctcata cacaaactgg ctgacgccct
      181 tttccgcgct ttgccggaaa atttccgcct gctgatccgc cggcagcgcc acgaggatgt
      241 ccggcttcac gccgcgcggg tagatcgcct ccgcgtccgg cacggtcgct tccgacaccg
      301 cgattttcag cgtcactccc ccgcccagcg gcacttcgga aaattccacc gcctggcccg
      361 ccgtggacgt tcccacaatc atggcccgcg cctgccgccg caggacggcc gcgaggattt
      421 cgccggcgcc ggcggtttga cggtccgtca gcaccaccac ctttccggtg aaaaccgaat
      481 cctgattcga ggtgaaaatg cgctcctgct tcgcgctcgg tttccgcacc cggaacaaca
      541 accggccctt ctcgcagaag cgccgggcga acgcggcggc ctgttcgtaa tcggcattgt
      601 aaccagccga gcggagatcg agcaccacgg catggacgct cttttccttg aatccggcca
      661 gcaccgcgtc cagctgggtc agcgtatccg catccagctt gccgaggcga atgtagccca
      721 cctgggaatc cagcacctcg gcgaggaacc ggcccggcgc cgcggcggcc ggtttggcgg
      781 attcgaggta gagatgggcg cccggtccga gccggtcgag caggccctcc agccgcgcgc
      841 ggcgcagttc ctgatcgttc agcgccgcgg ggttgaggaa ctgggtcttg agcagatcga
      901 ccgcctgatc cagctgcgtg tcattgagct ggttcaccag atccgccgcg ggcgccggcg
      961 ttcccgcggg cttgggcgaa ggcgtggccg cgggggttgg agccggcgtc ggcgagggtt
     1021 gctgcgccag taaagtcagc gagggaagca gcagcgcgcc ggcgaatgga agcagtttca
     1081 tgggcgggaa agcagtccaa tgtccgtgcg gcagaagccg ttttcaaagc ggattttttc
     1141 caccccggca tacgcccgcg cccgcgcttc ggcgagcgat gcgcccagcg cggtcacgcc
     1201 cagcacgcgg ccgccggaag tcacgagatg gctgccggca tcgcgcttcg ttccggcgtg
     1261 aaacaccttg atttcgggca gcgtttcggc ttcggcgatg ccggtgattt cgcggccctt
     1321 gacgtagtcg ccgggataac cgccggaggc ctggatgacg caaaccgcct cgcgcgcgtc
     1381 ccattccggg cgcgcgtccc ggagccgccg ccgcacggtg gcttccagca ggtcgagcaa
     1441 atcgctttgc agccgccgca tcagcacctg cgtttcggga tcgccgaagc ggcagttgaa
     1501 ttccaaaacc ttcgggccgg cgggcgtaat catcagaccc gggaaaagca tgccgcggaa
     1561 atccaggcca tcggctttga gcccggcgac gaagcggtcc agcacctccg cgcgaaccgt
     1621 ccgcagcagc tcggggccgg cggaatgcga ggggctgatc gtgcccatgc cgccggtgtt
     1681 cgggccgatt ccgccatcga acgcctgctt gtgatcccgg cagtccggga acagcagcca
     1741 ggattcgcca tccaccagcg cgtgaatgga gcattcgacg ccggtcagga attcctcgac
     1801 gaccacgcgg tcgcccgccg cgccgaattc gcgtttttcc atgaggcggt caatcgccgc
     1861 cgccgcttcc gcccggtcct gggcgatgat gacccccttg ccgagcgcca gcccgtcggc
     1921 cttgatcacg agcggataag ccgcctgttc gcaataatca cgcgcctccg ccgcggcggt
     1981 gaattcccgg taatccgcgg tcggaatgcc gtggcgccgc atgaaatcct tcgcgaaggc
     2041 cttggaggac tccagccgcg ccgccgaggc cgagggcccg aaaatcgcga gtccccgttt
     2101 ttcgaacgcg tccaccaccc cggccgccag cgtgtcgtcc ggcccgacca cggtcaggtc
     2161 gatgccctcc gcttccgcga agtccgcgag ggcggaaatc tcgccggcgc cgatgcccac
     2221 attcgtggcg aattcgccgg tgcccgcatt gccggggg
//
LOCUS       DATDAY010000251         2340 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_1926868, whole genome shotgun sequence.
ACCESSION   DATDAY010000251 DATDAY010000000
VERSION     DATDAY010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2340)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2340)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2340
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1926868"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..521)
                     /locus_tag="VIS74_05015"
     CDS             complement(<1..521)
                     /locus_tag="VIS74_05015"
                     /inference="COORDINATES: protein motif:HMM:NF021778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HEY5792636.1"
                     /translation="MKAWFFAAMVMASPVRGQGMDAYIGTYTTPDAPGGIYAAQFDPE
                     TGKWREPRLAAKAKNPAFLALGRKTLYAAIETDPGAVRAYAIGPRRTLTFLNEQPSGG
                     DGACHVSVDAAGKHVLIANYGGGSAAVFPVRADGSLGARSDLAAFQGSGPNPRRQRKS
                     YGHAVNAWGDFVYA"
     gene            complement(518..>2340)
                     /locus_tag="VIS74_05020"
     CDS             complement(518..>2340)
                     /locus_tag="VIS74_05020"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012403.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEY5792637.1"
                     /translation="AVLKFFATELPALRQVWQVREGERFQHVTRDLVRIEPQLAMQEA
                     SDGWLDMRVHFAAGKHAVFSMPEIQQLLRGGQGHVRLKDGRIAVADREQLHDLEEVLR
                     DCDPRQERGAYRVPAVHRGYLEASLAGWKGNEGGTAAAVPPPLGSLRERLRPYQVTGA
                     QWLLAHARAGRGALLADEMGLGKTIQTLAALEKMEGPKLVVCPSSLVWNWKREAAQFL
                     PHFRVLTLDGPSREKRFAEIGAQDLIVTSYALLRRDAERYRGLAFAAVVLDEAQHIKN
                     PDSQNARAAVSLRAGSRFILTGTPIENSVRDLWSLFEFLLPGYLGSRQDFKERYETPI
                     LNGERGGVWRRLSRRVQPYLLRRRKSEILTELPDKIEQIIEVELTEKQKATYDQLQQA
                     ARSQVDQLNETGAPGAARMRVLTALLRLRQACCDLRLFGGELPMEAASAKLTALLELL
                     EEARDGGHRTLVFSQFTSMLDWIGAALTEREIAYCRLDGGTKDRQRVVERFQNDAAIP
                     VFLISLKAGGVGLNLTAADTVIHFDPWWNPAVEAQATDRAHRIGQKRVVTSIKLIARD
                     TIESRVLSLQQKKRELLEGALDAEAALEGLNAEDLRELVA"
BASE COUNT          387 a          829 c          720 g          404 t
ORIGIN      
        1 gcatatacaa aatcccccca cgcgttcacc gcatgaccgt aggatttccg ctggcgtcgg
       61 gggttggggc cggagccttg aaaggccgcc agatccgacc gggcgcccag cgaaccgtcg
      121 gcccgcacgg gaaaaaccgc cgcgctgccg cctccgtagt tggcgatcag gacatgcttc
      181 ccggcggcgt ccaccgacac atggcaggcg ccgtccccgc cggaaggctg ttcattgaga
      241 aaggtgagcg ttcgccgcgg accgatggcg taagcccgca ccgcgccggg gtcggtttcg
      301 atggcggcat acagggtctt ccgccccagc gccaggaagg ccggattctt cgctttcgcc
      361 gccagccggg gctcccgcca ttttcccgtt tccggatcga attgcgcggc gtaaatccca
      421 ccgggcgcgt ccggggtcgt gtaggtgccg atataggcgt ccatcccctg cccccggacg
      481 ggcgaggcca tcaccatcgc ggcgaaaaac caggccttca tgccacgagc tcccgcaggt
      541 cctcggcgtt gagcccctcc agcgccgcct ccgcgtccaa ggcgccttcc agcagctcgc
      601 gtttcttttg ctggaggctc aatacccgcg attcgatggt gtcccgcgcg atgagtttga
      661 tggacgtcac cacgcgcttc tggccgatgc ggtgcgcccg gtcggtcgcc tgggcttcga
      721 cggcgggatt ccaccatggg tcgaaatgga tgacggtatc cgccgccgtc agattcagcc
      781 cgacgcctcc cgccttgagg ctgatgagaa agacgggaat ggccgcatcg ttctggaatc
      841 gctccaccac ccgctggcgg tccttcgtgc cgccatccag ccggcaatag gcgatttccc
      901 gctccgtgag cgcggcgccg atccagtcga gcatggaagt gaactggctg aaaacgagcg
      961 tgcgatgccc gccgtcccgc gcctcctcca gcaactcgag cagagccgtc agtttcgccg
     1021 aagcggcttc catcggcagc tcgccgccaa acaggcgcag gtcgcaacac gcctgccgga
     1081 gccggagcag ggcggtcagc acccgcatcc gcgccgcgcc cggcgcccca gtttcgttca
     1141 gctggtccac ctgggagcgc gcggcctgct gcaattgatc gtaggtcgcc ttttgttttt
     1201 cggtcagttc cacttcaata atttgctcaa ttttatccgg caattccgta agtatttccg
     1261 acttccggcg gcgcagcagg taaggctgga cgcggcggga caggcgccgc cagactcctc
     1321 ccctctcccc attgaggatc ggcgtttcgt aacgttcctt gaaatcctgc cgggagccga
     1381 ggtagccggg caggaggaat tcgaacagcg accagagatc gcgcacggaa ttctcgatcg
     1441 gcgtgccggt caggatgaac cggctcccgg cgcggaggga aaccgccgcg cgggcgttct
     1501 ggctgtccgg atttttgatg tgctgcgcct cgtccagcac gacggcggca aaggccaggc
     1561 cgcgataccg ctcggcgtcc cggcgaagca gcgcgtagct ggtcacgatc agatcctgcg
     1621 ccccgatttc ggcgaaccgc ttttcccggc tgggcccgtc cagcgtgagc acgcggaaat
     1681 ggggcagaaa ctgcgccgcc tcccgcttcc agttccacac cagcgaggaa gggcagacca
     1741 ccagcttcgg tccctccatt ttttccaacg cggccagggt ttggatcgtc ttgcccagcc
     1801 ccatctcgtc cgccagcagc gccccgcggc cggcccgcgc gtgcgccagg agccattggg
     1861 cgccggtgac ctgataggga cgcagccttt cccgcaggga tcccagcggc ggcggaaccg
     1921 ccgccgccgt tccaccctca ttccccttcc agcccgccag gctggcttcc agatacccgc
     1981 ggtgaacggc gggaacgcga tatgcgcccc gttcctgccg gggatcgcag tcgcgcaaca
     2041 cttcctccag atcgtgaagc tgctcgcgat ccgccacggc gatccggcca tccttcaacc
     2101 gcacatgccc ctggccgccg cggagcaatt gctggatttc cggcatggaa aagaccgcgt
     2161 gtttcccggc cgcgaaatgg acgcgcatgt ccagccagcc atccgacgcc tcctgcatcg
     2221 ccagctgcgg ctcgatgcgg accaaatccc gcgtcacgtg ctggaaacgc tccccttccc
     2281 gcacctgcca gacctggcgg agcgccggca gctcggtggc gaaaaacttc agaacggctt
//
LOCUS       DATDAY010000252         2667 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_46641, whole genome shotgun sequence.
ACCESSION   DATDAY010000252 DATDAY010000000
VERSION     DATDAY010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2667)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2667)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2667
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46641"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..621
                     /locus_tag="VIS74_05025"
     CDS             <1..621
                     /locus_tag="VIS74_05025"
                     /inference="COORDINATES: protein motif:HMM:NF014674.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792638.1"
                     /translation="RYFPDPDLLPVKTEKLVEVARARLPELPWEKRERFVRDFGVSEY
                     DAGVIANDLLLSAYFEAAALGARKPKNIANWILNDLQSALSASSKVISDCPIPPEALD
                     ELVNLVDAGSINGKQAKEVFGEMFATGKRAAVIVEEKGLKQESDTGALEAFCDEVIAA
                     NPGPVADYRSGKTAAVNFLKGQVMKLSKGKANPAVVGELLEKKLRA"
     gene            647..1108
                     /locus_tag="VIS74_05030"
     CDS             647..1108
                     /locus_tag="VIS74_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006983766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY5792639.1"
                     /translation="MAANPIPTGYENAIPYLCCRNAAELLEFYQQAFGAKEAYRLRMP
                     DGKIGHAEIEIGTARVMLSDEFPEWGNLSPKTLGGTGGNVMIYVEDVDAFCRRAEAAG
                     AQIHMPPTTHFYGDRSCKLIDPSGHVWVFATHVEDVSPEEMQKRCDAEFGG"
     gene            complement(1206..1955)
                     /locus_tag="VIS74_05035"
     CDS             complement(1206..1955)
                     /locus_tag="VIS74_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HEY5792640.1"
                     /translation="MDWITNPELWVAFLTLCGLEIVLGVDNIIFISILAGKLPPGQQA
                     KARFIGLALAMITRVLLLLSISWMTKLTTPLFALFAHPVSGRDLILILGGLFLVAKST
                     HEIHGKLEGSEEHGAVRAAASFTATIIQIILLDIVFSLDSVITAVGMVNNIPIMVAAV
                     IVAVIFMMFFSGPISGFVDRHPTVKMLALSFLLLIGVTLIADGFGQHISKGYIYFAMG
                     FSVFVEFLNLKLRKKSAQPVKLHQPFAKKHS"
     gene            2071..2307
                     /locus_tag="VIS74_05040"
     CDS             2071..2307
                     /locus_tag="VIS74_05040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792641.1"
                     /translation="MTRMTIYLDADTQKAVEAAAAREELPVSHWAREHLREAAGNALR
                     PHSLDSFYGAIADDSFREPDELPSQGDGKRLTFD"
BASE COUNT          590 a          739 c          824 g          514 t
ORIGIN      
        1 cgctatttcc ccgatccgga tttgctgccg gtgaaaacgg aaaaactcgt cgaggtcgcc
       61 cgcgcgcgcc tgcccgaatt gccctgggaa aagcgcgagc gttttgtgcg cgatttcggg
      121 gtgagcgaat acgacgccgg agtcatcgcg aacgaccttt tattgtccgc ctatttcgaa
      181 gccgcggcgc tcggggcgag gaagccgaaa aacatcgcga actggattct gaacgatctc
      241 cagagcgcgc tgtcggccag ctcgaaggtc atttccgact gcccgattcc gcccgaggcg
      301 ctggacgaac ttgtcaacct cgtggacgcg ggaagcatca acggcaagca ggccaaggaa
      361 gtcttcggcg agatgtttgc gaccggcaaa cgcgccgccg tcattgtcga ggagaagggc
      421 ctcaagcagg aaagcgacac cggcgcgctc gaggcgttct gcgacgaagt catcgccgcg
      481 aaccccggcc cggtggcgga ttaccggtcc ggcaaaaccg ccgcggtgaa cttcctcaag
      541 ggccaggtca tgaagttgtc gaaaggcaag gccaatccgg cggtcgtcgg ggaattgctg
      601 gagaaaaaac tgcgggctta aattccgaag agcccgaaaa agattcatgg ctgcgaaccc
      661 cattcccacc ggctacgaaa atgcgattcc ttatctctgc tgccggaacg cggcggagtt
      721 gctcgagttt tatcagcaag cgtttggcgc gaaggaggcc tatcgcctcc ggatgccgga
      781 tggcaaaatc ggccacgccg aaatcgagat cggcactgcc cgcgtgatgc tgtcggacga
      841 gtttcccgaa tggggcaatc tcagcccgaa gacccttggc ggcacgggcg gaaatgtcat
      901 gatttacgtc gaggacgtgg atgccttctg ccggcgggcg gaagcggccg gcgcgcaaat
      961 tcacatgccg ccgaccacgc atttctacgg cgaccgcagc tgcaagctca tcgatccctc
     1021 cggccacgtc tgggtcttcg cgacgcacgt ggaagacgtc tccccggagg agatgcagaa
     1081 acgctgcgac gccgagttcg gcggttaaga gggtccgccg gtttttacca tgaaggacat
     1141 gaagtgcatg aaggttgatt tcatgagtag ctcttcaatc gcttcatgtc cttcatggta
     1201 aatcttcagg aatgtttctt ggcaaacggc tgatgcagtt ttaccggctg cgcggacttc
     1261 ttgcgcagct tgagattgag aaattccacg aagactgaga agcccatcgc gaaataaatg
     1321 tagccctttg aaatatgctg gccgaagccg tcggcgatca aggtcacgcc gatgagcagc
     1381 aggaagctaa gggcgagcat tttcaccgtc ggatgccggt ccacgaaacc gctgatcggg
     1441 ccggagaaaa acatcatgaa aatgaccgcc acgatgacgg cggcgaccat gatcgggata
     1501 ttgttcacca tgccgaccgc ggtgatgacc gagtccagcg agaacacgat gtcgagcagg
     1561 atgatttgga tgatcgtcgc ggtaaacgac gcggcggcgc gcaccgctcc gtgctcctcg
     1621 ctgccttcga gcttgccgtg aatttcgtgc gtgcttttgg cgaccaggaa caggccgccg
     1681 aggatcagaa tgagatcccg cccgctgacc gggtgcgcga acagggcaaa aagcggcgtg
     1741 gtgagtttcg tcatccacga aatcgagagc agcagcagca cccgcgtgat catggccagc
     1801 gccagcccga tgaaccgggc cttcgcctgc tgtcccggcg gcagcttgcc ggcgaggatg
     1861 gagatgaaaa tgatgttatc gacgccgagc acgatctcga gcccgcacaa cgtcaaaaat
     1921 gcgacccaaa gctccgggtt ggtgatccag tccatggagc gttggtttag cgggagccgc
     1981 cgtttcctac aagcgggaag caacccgtaa agaatcgggt taggaatccc aagctggtgg
     2041 tgcggctaaa aaaaatgact atttttagcc atgacccgaa tgaccattta tctggatgcc
     2101 gatactcaga aagcggtgga agcggcggcg gcccgggagg agttgcccgt atcccactgg
     2161 gctcgggaac atttgcggga agcggccggg aatgcattga ggccgcattc gttggattcc
     2221 ttttacgggg cgattgccga cgattccttc cgggaaccgg atgagcttcc gtcccagggc
     2281 gatggcaaac ggctgacatt cgattagcgg tgtctcgtgc ctcgaatcat gagtcagcca
     2341 cactgtgctt gaaattcggg gaacgccgat tggtctaaac gatcttttta tcgccgccgc
     2401 ggtccggtcc gcgggcgcaa ccttgatcac cgcgaacgaa agcgagtttc gccgtgcgcc
     2461 ggactcggaa aattggtgtt gaaacaggga gaacgggcgt cccgcccgtt tgggatttgg
     2521 gttactggcg ggacaccggt gcactcgggt agattccagc cgggaatttg catttcctcg
     2581 gggtgcttcg tggttaaatg acctctccca tgaatctcac tgtcaacgaa atctacgaat
     2641 cagtgcaggg cggaagcaca cgcgcat
//
LOCUS       DATDAY010000253         2929 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_2273966, whole genome shotgun sequence.
ACCESSION   DATDAY010000253 DATDAY010000000
VERSION     DATDAY010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2929)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2929)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2929
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2273966"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..784)
                     /locus_tag="VIS74_05045"
     CDS             complement(<1..784)
                     /locus_tag="VIS74_05045"
                     /inference="COORDINATES: protein motif:HMM:NF019292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HEY5792642.1"
                     /translation="MLNYIWLGLLIGAVLLGGFFGKLPDVANAAFESSKKAVMDIALP
                     LAGMMALWLGIMRLAEKSGLVLILAKALRPVLRWLFPEVPANHPAMGSMLMNMAANML
                     GLSNAATPLGLRAMQDLEKLNPRPGIASNPMCTFLAINTSSIQLIPASAVAILAAAGS
                     KNPSAIIGTAFLATLCSTVAGVTAVKLLERLPGYRLPAPEPENQRKVDEALKLEAAEA
                     IVESQPMAVWARLVLALFAVVMLYFGYQAYVTIAAAGTDKLAI"
     gene            complement(844..1206)
                     /gene="queD"
                     /locus_tag="VIS74_05050"
     CDS             complement(844..1206)
                     /gene="queD"
                     /locus_tag="VIS74_05050"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010434567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HEY5792643.1"
                     /translation="MKVRITKDFEFESAQTLPKVPADHKCGKMHGHSFKVEITVAGEV
                     NPETGWFYDHAEISQAMRPLVDRLDHAYLNDIEGLENPTIENMCAWFWNILAPQLPGL
                     AEITIHETPRARCTYRGE"
     gene            complement(1273..1917)
                     /locus_tag="VIS74_05055"
     CDS             complement(1273..1917)
                     /locus_tag="VIS74_05055"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEY5792644.1"
                     /translation="MNLTVNEIYQSVQGESTWAGLPCVFVRLTFCDLRCTYCDTAYAF
                     YEGRKRPVEEIVQEVLAFDCPLVEITGGEPLLQKNVLPLMTALCDAGKTVLIETSGAH
                     DISRIDPRVHRIMDLKTPSSGECGRNLFSNLAHLQQRDEVKFVIGTREDYEWTRGQIL
                     THRLNEIVHAVLLSPIFGKIQARDIVDWMLADKLPARFQLQMHKFIWEPTKKGV"
     gene            2352..2732
                     /locus_tag="VIS74_05060"
     CDS             2352..2732
                     /locus_tag="VIS74_05060"
                     /inference="COORDINATES: protein motif:HMM:NF027602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4926 domain-containing protein"
                     /protein_id="HEY5792645.1"
                     /translation="MSAWIDYLAWTALHDGFFHSPESSGSANPERLDCAHRRKIPSFG
                     IVLRAINSPVKTTPAKLLDVVAVLVDKPAFQLAAGQVGTIVEVLAPDVFEVEFCNSQG
                     NTLGFAELRRDEFLILRHEPALAA"
BASE COUNT          600 a          877 c          863 g          589 t
ORIGIN      
        1 cgatcgcgag cttgtccgtc cccgcggcgg cgatcgtcac gtaggcctga tagccgaaat
       61 acagcatcac gacggcaaac aacgccagaa ccagccgggc ccagaccgcc atcggctggg
      121 attccacgat tgcctccgcc gcctccagct tcaaggcctc gtccaccttc cgctgatttt
      181 ccggctcggg cgcgggcagg cgataacccg gcagccgctc cagcaatttc accgcggtca
      241 cgccggcaac cgtcgaacaa agggtcgcca ggaacgccgt gccgatgatc gcggacggat
      301 tcttcgagcc cgccgcggcg aggatcgcca ccgccgacgc ggggatgagc tggatcgagc
      361 tggtattgat ggccagaaag gtgcacatcg ggttgctggc gatgcccggg cgggggttga
      421 gcttctccag atcctgcatc gcgcgcaggc cgagcggcgt ggcggcattg ctcaggccca
      481 gcatgttcgc cgccatgttc atgagcatcg aacccatcgc ggggtggttc gcgggaacct
      541 cggggaacag ccagcgcagc accggccgca gcgccttcgc caggattaat acgagcccgg
      601 atttttccgc gagccgcatg atgcccagcc agagcgccat catccccgcc agcggcaggg
      661 cgatgtccat gaccgctttc ttgctcgact caaacgcggc gttcgcgacg tcgggaagct
      721 tcccgaagaa gcccccgagc aacaccgcgc cgatcagcag gccgagccag atgtagttta
      781 gcatgatgaa ggaaaaatga cgaaggacga agcccgaagc acgaatggca aattgttcgc
      841 catttactcc ccccgatacg tgcagcgcgc gcgcggcgtt tcgtgaatcg tgatctccgc
      901 caggcccggc agttgcgggg cgagaatgtt ccagaaccac gcgcacatgt tttcgatggt
      961 tggattttcc aggccctcga tgtcgttgag atacgcgtga tccagccggt ccaccagcgg
     1021 gcgcatggcc tggctgattt ccgcgtgatc gtaaaaccag cccgtttcgg gattcacctc
     1081 gccggccacg gtgatctcca ccttgaagct gtgcccgtgc attttgccgc atttgtgatc
     1141 ggcgggcacc ttgggcaggg tctgggcgga ttcgaattcg aaatccttgg tgatgcgaac
     1201 tttcattgga tggattcagg cagctgcgac cgactctgca atcgaaaatt gaaaatcgcc
     1261 aatcgaaaat ccctacactc cctttttcgt gggttcccaa atgaacttgt gcatctggag
     1321 ctggaagcgg gccggcagct tgtcggcgag catccaatcc acgatgtcgc gcgcctggat
     1381 cttgccgaaa atggggctga gcagcacggc gtggacgatt tcattcagcc ggtgggtgag
     1441 aatctgcccg cgggtccact cgtaatcctc gcgcgtgccg atgacgaact tcacctcgtc
     1501 gcgctgctgt aggtgcgcga ggttggaaaa gagattccgc ccgcattcgc cgctggaggg
     1561 cgttttgaga tccatgatgc gatgcacccg cggatcgatc cgggaaatat catgcgcgcc
     1621 gctggtttcg atcaacacgg ttttgccggc atcgcacaat gccgtcatga gcggcaggac
     1681 attcttttgc agcaacggct cgccgccggt gatttccacc agcgggcagt cgaacgccag
     1741 cacttcctgg acgatctcct ccaccggccg cttccggccc tcgtagaacg cgtaggccgt
     1801 gtcgcaatac gtgcagcgca aatcacagaa cgtcagccgg acaaagacac acggcaaccc
     1861 cgcccaggtg ctttcgccct gcacggattg atagatttcg ttgacggtga gattcatcgg
     1921 aatcggccaa tgtaaccgcg aaccccgcga agggcacgca gagattttcc agggcgcacc
     1981 ggcatccggc atgcgaccca aatcccaaac gggcgggacg cccgttcccc ctggctcagg
     2041 caggaattgg attggcaatg aaagcgaccg aaagcgacta tgcaaatcaa tgatctacct
     2101 cgaccacaat gccacgaccc cggttttgcc cgagacattt aaaaccaagc ggccgtccct
     2161 ccgcaaaggc cctgcatgaa actactggca aatgcggttg ttgatttcgc gaaattgcgg
     2221 gattactgcc tcagtccgaa tcatccggaa ggcaaacaca aggcgagagt cttttcggcg
     2281 gcacttggca tttgtgcaga tgacgccgaa tggcttcggg ccgagctgct tgccgcagtt
     2341 cttcgggagg aatgtcagcc tggattgatt acctcgcatg gacagcgcta cacgatggat
     2401 ttttccattc gccggaatca tcgggaagcg cgaatccgga gcggttggat tgtgcgcatc
     2461 ggagaaaaat tccctcgttt ggtatcgtgt tacgtgctat caattcccca gtgaaaacaa
     2521 ctcccgccaa actgcttgat gtggttgccg tccttgtgga caagccggca tttcagctgg
     2581 cggcgggcca ggttggcacc attgtggagg tgctggcacc cgatgtcttc gaagtggagt
     2641 tttgcaattc gcaaggaaac accctcgggt ttgcagaatt gcggcgggat gaatttctca
     2701 ttctccggca cgaacccgcg cttgcggctt agtaattcgg ttcgcttggc ttgaaataca
     2761 aactctgccg tcggaccgtg ctctccgaag acctgccgcg ggaaggcatt tgcagtggcg
     2821 acgatcattg aattttatga cgttccgctc gaagttttta atgcggccgg cgagaccatt
     2881 gccgttgtca cggtccgcga atcacagatc gagccgctcc ggcgcgatg
//
LOCUS       DATDAY010000254         4220 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_2008603, whole genome shotgun sequence.
ACCESSION   DATDAY010000254 DATDAY010000000
VERSION     DATDAY010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 4220)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4220)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4220
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2008603"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..921)
                     /locus_tag="VIS74_05065"
     CDS             complement(<1..921)
                     /locus_tag="VIS74_05065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEY5792646.1"
                     /translation="MKGRLGSLAGCLLLAALGLARADIQIDPDPKDETLEPYTSLTIH
                     FPKDLVPPEQIDAAKAQSPIIATPPLNGQFIWRTQSQGVWSVDGPRIPGTTYRFTLRP
                     DLRDLEGNPLAGADWHADYSTNPLRVDADYGERERLSAQPQVPLEFNFLIHLPSVPEG
                     AWIQDRATRQRFPVEVMLNRPAAEDLTGAVEEVKLPELPASLEFRVRPREPLPVGRMY
                     DLVVDGVTDAHGGKTLPYPRVFPLGRTRPLEVDYVAARNWPTDQPKIEVKFRTPIPDA
                     ALPAGSIQVTPAVPGLHFRVADECVFLEGKF"
     gene            complement(918..2249)
                     /locus_tag="VIS74_05070"
     CDS             complement(918..2249)
                     /locus_tag="VIS74_05070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792647.1"
                     /translation="MAALPWTGLAAYGANLAEQIKLARTDSDSNAEIELLRRSLDQNP
                     NDAAARERLVRLWLEVRDFAMAETALAEWKGAPPEMTVWVRAQILAEREGKLAEAIAS
                     LRESVKLSPRHWENRELLAAYLGRAVRWEEQIQVLGQLLAERPDSTHYLSRAEARKRL
                     EDYAGAILDARAAQKLSPDGSRVKATVPGYERLAQVLPVIDSTTRQLQTQPENVDLLL
                     ARGAALQYGEVPRRALTDAEAALKQDPGSAYAILLKARALLASGQKPSYELADEYPVN
                     LSAPVESPPIAEGLIRADAAVRADPKNAAAWAQRAQRFNADQQSRRALLDAQKALSLD
                     GKNPAALLEAIYAAGKSGDPDAAADYFRRLEQVKPPDETLALALATLVESSFAATNFQ
                     QAVEFADRALKLRETESLLRYKAQALRRLGYDEEAEAVMTRAEKLKGKAKP"
     gene            2287..3573
                     /locus_tag="VIS74_05075"
     CDS             2287..3573
                     /locus_tag="VIS74_05075"
                     /inference="COORDINATES: protein motif:HMM:NF015451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY5792648.1"
                     /translation="MSSHPTSIPEQSGAKKRRRIGVVGGGAAGFFAAITAAQAESRPE
                     VVLLEKSSQFLSKVRISGGGRCNVTHACFDPRALSQRYPRGERALISPFTRFQPRETV
                     EWFARRGVALKTEADGRMFPVTNSSGTIVDCLLEEARRSGVRLGLKEGVEEVVRRESG
                     GFDLTLSNGEILACDALLLATGGCRTPAAAKLAQALGHTLAPPVPSLFTFHIEVPWLK
                     RIPGVSVAAVEAEIPGTKLRERGPLLVTHWGLSGPVILRLSAWGARELHERDYQFPLR
                     IRWLPDASRESLAALLQKPRETQPTKLVLNTPLAGLPARLWEELVALAGIYPDTPWTR
                     LSRHGARQLAALLYSTEFPVTGKSLNKDEFVTCGGVPLREVDFRTMESRVCPGLYFAG
                     EVLDVDGITGGFNFQAAWTTGWIAGNAMSGATPPDC"
     gene            complement(3554..4141)
                     /locus_tag="VIS74_05080"
     CDS             complement(3554..4141)
                     /locus_tag="VIS74_05080"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEY5792649.1"
                     /translation="MKEESASVEFQIGQRIRVLRMQRDVTLDQLAEEVKLTKGQLSKI
                     ENGKVSSPVSTLTRIAAALSVEPGAFFQAANHDPRAAFVRKSDRKVIVGRGSKIGHSY
                     ESLAYGLPFKKDFEPYVMRIEEKKIDPAKNVFRHPGHELLFMLKGKMDYRHGDKTYHL
                     EPGDSLFIDGTIEHGPVKVYNPPVEFLSIISNPAE"
BASE COUNT          700 a         1412 c         1344 g          764 t
ORIGIN      
        1 gaatttccct tcgagaaaca cgcattcgtc cgccacgcga aaatgcaggc cgggcaccgc
       61 gggcgtgacc tggatggagc ccgccggcag tgccgcgtcc ggaatgggcg tgcggaattt
      121 gacctcgatc ttcggctgat ccgtcggcca gttccgcgcg gccacgtaat ccacctccag
      181 cggccgggtg cggcccagcg ggaaaacgcg cggatacggc agcgtttttc cgccatgggc
      241 gtcggtgacg ccgtccacca caaggtcata catccgcccg accggcagcg gctcgcgcgg
      301 ccggacgcgg aattcgagcg aggcgggcag ctccggcaat ttcacttcct cgacggcgcc
      361 ggtgagatcc tccgccgcgg gccggttcag catgacctcc accggaaacc gctgccgcgt
      421 ggcccgatcc tgaatccagg cgccttccgg gacgctggga agatggatga ggaagttgaa
      481 ttccaacggc acctgcggct gcgcgctcaa gcgctcgcgt tcgccatagt ccgcgtccac
      541 ccgcagcggg ttggtcgaat aatccgcgtg ccaatccgcg ccggccagcg gattgccttc
      601 gagatcccgc aagtccggcc gtagcgtgaa gcggtaggtc gtgccgggaa tgcgcggacc
      661 gtccaccgac cagacgccct gcgactgcgt gcgccagatg aattggccat tgagcggcgg
      721 cgtggcgata atcggcgatt gcgccttggc cgcgtcaatc tgctccggcg gcaccagatc
      781 cttcggaaag tggatcgtca gggacgtgta gggctccagc gtctcgtcct tgggatcggg
      841 atcgatctgg atgtccgcgc gggccaggcc cagcgcggcc agcaggagac accccgccaa
      901 actgccgaga cgtcccttca tggcttcgcc tttcctttca atttctccgc gcgtgtcata
      961 accgcctccg cctcctcgtc ataaccgagc cggcgcagcg cctgcgcctt gtagcgaagc
     1021 agcgattccg tctccctgag tttcagggcg cgatcggcga attcgacggc ctgctggaaa
     1081 ttcgtcgcgg cgaaactgct ttccacgagt gtcgccaacg ccagagccag cgtttcatcc
     1141 ggcggcttca cctgctccaa tctccggaaa taatccgccg cggcatccgg atcgcccgat
     1201 ttccccgcgg cgtaaatggc ttccagcagc gccgccgggt ttttgccatc caggctcagc
     1261 gccttctgcg catcgagcag cgcgcggcgg ctttgctggt cggcgttaaa acgctgcgcc
     1321 cgctgcgccc aggcggccgc gtttttgggg tccgcccgga ccgcggcatc cgcccggatc
     1381 agcccctcgg caatcggagg ggattccacc ggcgcgctga ggttgaccgg gtattcatcg
     1441 gccagttcgt aggaaggttt ttggccggaa gccagcagcg cgcgggcctt caggagaatc
     1501 gcgtaggccg agccgggatc ctgcttgagc gccgcctcgg cgtcggttag cgcccggcgc
     1561 gggacttcgc catattgcag agccgcgccg cgcgccagaa gcaggtccac gttctccggc
     1621 tgagtttgca gttggcgggt ggtcgaatca atgaccggca gcacctgggc cagccgctcg
     1681 taacccggca cggtcgcctt cacccggctg ccgtccgggc taagcttctg agccgcgcga
     1741 gcgtccagga tcgccccggc gtaatcctcc aaccgcttcc gggcctcggc gcggctgagg
     1801 taatgggtag aatccggccg ctcggcgagc aattgcccca gcacctggat ctgctcctcc
     1861 cagcgcaccg cccgacccag ataggccgca agcaattccc ggttttccca atgacgggga
     1921 gacaatttca ccgattcccg caaactcgcg atagcctcgg ccagcttgcc ctcgcgctcg
     1981 gcgagaattt gcgcccgcac ccagaccgtc atttcgggcg gcgcgccctt ccattccgcc
     2041 agcgcggttt cggccatggc gaaatcccgc acctccagcc acaggcggac caatcgctcg
     2101 cgggccgcgg cgtcgttcgg attttgatcg agggaacgcc ggagaagctc gatttcggca
     2161 ttggaatccg agtcggttct tgccagcttg atctgctccg cgagattggc gccgtaagcc
     2221 gcgaggccgg tccatggcag ggctgccatg accagcattc gggccgttga tcgcagggcc
     2281 gcaaccatgt cctctcatcc aacctcaatc cccgaacaaa gtggagcgaa aaagcgccgc
     2341 cgcatcggag tcgtgggcgg cggggcggcc ggtttttttg cggccatcac cgccgcgcag
     2401 gcggagagcc ggccggaggt cgtcctgctg gaaaaatcct cgcaattcct cagcaaggtc
     2461 cgcatctccg gcggcgggcg ctgcaatgtc acgcacgcct gcttcgaccc ccgggccttg
     2521 agccagcggt atccccgcgg cgaacgcgcg ctcatttcgc cgttcacccg gtttcagccc
     2581 cgggagacgg tcgaatggtt cgcgcggcgc ggggtggcgc tcaagacgga ggcggatggc
     2641 cggatgttcc cggtcaccaa ttcgtcggga accatcgtgg actgtctcct ggaggaagcg
     2701 cgccgctcag gcgtccgcct cggcctgaaa gagggcgtcg aggaggtggt ccgccgcgaa
     2761 tccggcgggt tcgatctcac gctgtccaat ggcgaaatcc tcgcctgcga cgcgctgctg
     2821 ctcgcgaccg gcggctgccg cacccccgcc gccgcgaagc ttgcgcaagc cctcggccac
     2881 acgctcgcgc cgccggttcc ctcgctgttc acgtttcata tcgaagtccc ttggctgaaa
     2941 cgcattccgg gcgtctcggt agccgccgtc gaggcggaaa ttcccggcac gaaattacgc
     3001 gaacgcgggc ccctgctcgt gacgcattgg ggtctgagcg ggccggtgat cctccggctt
     3061 tccgcctggg gagcgcggga gcttcacgaa cgggattacc aatttcccct gcgcatccgc
     3121 tggctgccgg acgcgagtcg ggaaagcctc gcggcgctgc tgcaaaaacc ccgcgaaacc
     3181 caacccacga agctggtttt gaatactccc ctcgccggcc tcccggcgcg gctgtgggag
     3241 gaattggtgg cgctggccgg gatttacccc gacaccccct ggacccgcct gtcccgccac
     3301 ggcgcgcggc aactggcggc gctgctctat tccaccgaat tccccgtcac cggcaagagc
     3361 ctcaacaagg atgaattcgt gacgtgcggg ggcgttccgc tgcgcgaggt ggatttccgg
     3421 acgatggaga gccgcgtctg cccggggctg tatttcgccg gggaggtgct cgacgtggat
     3481 ggcatcaccg gcggcttcaa tttccaggcg gcctggacga ccggctggat cgcgggaaac
     3541 gcgatgagcg gcgctactcc gccggattgc tgatgatgct caaaaattcg acgggcggat
     3601 tgtaaacctt caccgggccg tgctcgatcg ttccatcgat gaacaggctg tcgccgggtt
     3661 ccaaatggta ggtcttgtca ccgtggcggt aatccatttt gcccttcagc atgaagagca
     3721 gctcgtggcc gggatggcgg aacacgttct tggcgggatc gatcttcttt tcctcgatcc
     3781 gcatgacata cggctcgaaa tccttcttga agggcaatcc atacgccagc gactcgtagc
     3841 tgtggccgat cttggaaccg cggcccacga tgaccttgcg gtcggacttg cgcacgaacg
     3901 cggcgcgcgg atcgtgattc gcggcttgaa agaaggcgcc gggctccacg gaaagcgccg
     3961 cggcgatgcg cgtcagcgtg gaaacgggcg aggaaacctt gccattttcg attttcgaaa
     4021 gctggccctt cgtcagcttc acttcctcgg ccaattgatc gagcgtcaca tcgcgctgca
     4081 tgcgcaggac gcgaatacgc tggccgatct gaaactccac tgaggcggat tcctctttca
     4141 ttccgcggga tactgacaga ggaaaccgcc gcatggcgag ttcggactgc ggcggaatgg
     4201 cacgtattgt gcttccctcc
//
LOCUS       DATDAY010000255         2076 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_1609882, whole genome shotgun sequence.
ACCESSION   DATDAY010000255 DATDAY010000000
VERSION     DATDAY010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2076)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2076)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2076
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1609882"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(211..>2076)
                     /locus_tag="VIS74_05085"
     CDS             complement(211..>2076)
                     /locus_tag="VIS74_05085"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY5792650.1"
                     /translation="HYLNEKGPMPPALAVRVAIQVCRALSAAEKEGLVHRDIKPANLM
                     LMENEEGLRVKVIDFGLAKPSSLAGEADATLTIAGFVGTPYFASPEQLGEQDLDIRSD
                     IYSLGVTLWFMLSGRPPFAGTFASLIVQHLHSELPADHLRGVSPPLIEVLKKSLAKNR
                     EERFQTPADFRAALQECLPGLPETVVVTAPASLPENLETRLMDTRVPSEAPQSPSAPS
                     YAPGLETRTSGGRPNLLAAGAGLIALAALAIGGALWFTKPKPAAPAAAEATPAPPASP
                     VPVAVALAVSPSATPEASPEASVVPDAGSELEDSLELAKADELSRKIPEAILQYVALI
                     QKYPDDPRAKLRLDLLFQDLSEERSLIADPKPLSPADRAAAETAAETGNARAQLFLGG
                     ALRKSEPETAVKWLTLAANQKEPRAMVYLALMTFRGAGGLPRNPRRAIDLLTEANTLG
                     DKEAKPFLGDALLQRTGELAPFYQPARGIEILQESADLGSGLAMYKLGVCYDKGIGVA
                     RDEVTAFQWYERAYEQGLIQAAGNLGVCYLKGWGVPKNPSKAVQIFAEGAKSNDPDCL
                     YFYGGCLMDGLGTAKNLPLGKSYLAKAAALGHSNAIAACRERNIPFASTPDPAQP"
BASE COUNT          335 a          645 c          721 g          375 t
ORIGIN      
        1 cggacgaacc tacccagccc aggccggtgg ccccggccac gctgccggcg atcccgccgg
       61 cgattgctcc ggccgagccg gcttcgtcgc tggtcacgca gccgttcagg aaggtggcgc
      121 aaatccccgt ggcgagtaga aatccttttt tcatgggagg cgtagtttac aaacggccgg
      181 cttggccgga caagcctgtt ttggcgggcg tcagggctgc gccgggtccg gggtggaagc
      241 aaaggggatg ttccgctccc ggcaggcggc gatcgcgttc gaatggccca gcgcggcggc
      301 cttggcgaga tacgattttc cgagcgggag gtttttggcg gttccgagcc cgtccatgag
      361 acagccgccg tagaaataca ggcagtccgg atcgttgctc ttggcgcctt cggcgaagat
      421 ttgcacggct ttgctggggt ttttcggcac gccccagcct ttcaggtagc agacgccgag
      481 attgcccgcc gcctggatga ggccctgttc gtaggcgcgc tcataccatt gaaacgcggt
      541 gacttcgtcc cgggccacgc cgatgccctt gtcgtagcag acgccgagct tatacatggc
      601 cagcccgctg ccgagatcgg cggattcctg gaggatttcg attccccggg cgggctgata
      661 aaaaggcgca agctcgccgg ttcgctgcaa cagcgcgtcg ccgagaaagg gcttggcttc
      721 cttgtcgccg agcgtgttgg cttcggtcag cagatcaatg gcgcgccggg gattccgggg
      781 caatccgccc gcgccccgga aggtcatcag ggcgaggtaa accatggcgc gcggttcctt
      841 ttggttcgcg gccagggtca gccatttcac cgcggtctcg ggttcgcttt tccgcagcgc
      901 tccgcccaaa aagagctggg cccgcgcgtt gccggtttcg gcggccgttt cggcggcggc
      961 gcggtcggcg gggctcagcg gcttgggatc ggcaatcaag gagcgctcct cgctcaggtc
     1021 ctggaagagc aggtcgagcc gcagtttcgc gcggggatcg tccggatatt tttgaatgag
     1081 cgcgacgtat tgcagaatgg cttcggggat tttccggctc aattcatccg ccttcgcgag
     1141 ctcgagcgag tcctccagct ccgaaccagc gtccggaacg acggaggcct ccgggctcgc
     1201 ttccggagtg gcgctggggg aaaccgcgag ggccacggcc accgggacgg ggctggccgg
     1261 gggagccggc gtggcctcgg cggccgcggg cgcggcgggt ttgggtttcg tgaaccataa
     1321 cgcgccgcca atggccaaag ccgccagcgc gatcaatccg gcgcccgccg cgaggagatt
     1381 cggccttccg ccgctggtcc gggtttccag tcccggcgca tacgaaggcg cggatggcga
     1441 ttgcggcgct tcggagggaa cccgggtgtc cattagccgg gtttccagat tttccggaag
     1501 ggaggcgggc gccgtcacca ccaccgtctc gggcaggccg gggaggcatt cctgcagcgc
     1561 cgcgcgaaaa tcagcgggcg tctggaagcg ttcctcgcga tttttggcga gggatttttt
     1621 cagaacctcg atcagcggcg gcgaaacgcc tcgcaggtga tccgccggca gctcggaatg
     1681 caggtgctgg acgatgaggc tcgcgaaggt gcccgcaaag ggcggccggc cggagagcat
     1741 gaaccaaagc gtcacgccga gggaataaat gtccgaacgg atgtcgagat cctgctcccc
     1801 gagttgctcc ggactggcga aatagggcgt cccgacgaat ccggcgatcg tgagcgtggc
     1861 gtccgcctcc cccgccagcg agctgggctt ggccaagccg aaatcgataa ccttcacacg
     1921 caggccctcc tcattttcca tgagcatgag gttggcgggt ttgatgtcgc gatgcacgag
     1981 cccctccttt tccgcggcgg aaagcgcccg gcagacctgg atggccacac gcaccgccag
     2041 cgccggcggc atcggccctt tttcgttcag gtaatg
//
LOCUS       DATDAY010000256         6140 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_1701479, whole genome shotgun sequence.
ACCESSION   DATDAY010000256 DATDAY010000000
VERSION     DATDAY010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 6140)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6140)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6140
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1701479"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            28..2217
                     /gene="infB"
                     /locus_tag="VIS74_05090"
     CDS             28..2217
                     /gene="infB"
                     /locus_tag="VIS74_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEY5792651.1"
                     /translation="MAAKITTGKNEPYPSSRAKKPAFSPKPGAVPSTPAASEKPSDAP
                     PAPKAPVEAVSLIEPKKKIRRADLGDSGFKKPFLPTIGPVAPKVEPEPPKAAAPAPTP
                     APAPAPVVEDLISGKPKPAPVVEPKVEEPEAEVESAKIIHIKPPIIVRELAEALGIKA
                     FELIRELMALNIFPSINQSIEPDIAARICEKYGFIFEKERREKGAGIHRQEVVIVAPK
                     APEKPKEEELSLRPPVITMMGHVDHGKTSLLDAIRKARVAAGEAGGITQHIGAYSVER
                     DGQKITFLDTPGHAAFTAMRARGANVTDIVVLVVAADDGLMPQSIEAINHAKAAGATI
                     MVAINKIDLPSANPDRVKQQLQERDLTPEDWGGTTVICPVSATKGTGVPELLDMIHLQ
                     AEVLELKASSQADPRAIVIEAQVEAGRGPTATAIVKMGTLKVGQAFICGNYWGKVKQL
                     INDLGKPIKEAGPSTPIKVLGFTGLPNAGDELVVMDSERSARMLSEERLDALRTEKLA
                     VPQRATLESLFATIAGEQKKTLRVVLKSDVQGSMEALRGALEDIPSDKVDLNVIHAAV
                     GPISESDVLLASASNAVILGFSVKVENNASATAKREGVQIKLYSIIYELLDQVREAMA
                     GLLDPELRETVIGHAEVKQVFDLTKGKVAGCQVTDGRILRTGRARLLRRRQPIYDGGL
                     GTLRRFQDDVKEVRNGLECGIKLGDFSEYEVGDIIECYTLDKVPQTL"
     gene            2251..2622
                     /gene="rbfA"
                     /locus_tag="VIS74_05095"
     CDS             2251..2622
                     /gene="rbfA"
                     /locus_tag="VIS74_05095"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEY5792652.1"
                     /translation="MNNRLVRVCEVLKRELSTLMQKAMSFDAPLVSVAAVDITPDLKH
                     AHFYISAFGNDAQKRAALQTLEQHRPMLQHEVSKRVTLKNTPHFHFHLDNSIERGVRV
                     LQVMDELGLAEPPPKGKKRDS"
     gene            2619..3605
                     /locus_tag="VIS74_05100"
     CDS             2619..3605
                     /locus_tag="VIS74_05100"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HEY5792653.1"
                     /translation="MNAENASFAQIGAAIAAAKTLLVTSHLRPDGDALGSTLACALWL
                     RQLGKDVTAWNEDGVLEKFHYLPEWPLVTRPEGNPREFDAVILLDTSVKNRAGTCLDH
                     IAKAGVWINLDHHISNGRFGDLNYVDPTAPATGEILHQFLQSQNAAITPGIAANLFAA
                     ISTDTGSFQYAGTSPRTFRIAAELVEAGVDVAQLSRRMYDSQPRRRLDLLRHALNAAE
                     FACNDHAVSFTLTLADAERLGVVPEDNEGIIDQLRAVDTVVAAVFFEELPENRVRVSA
                     RSKDPRVDVCRICGLFGGGGHPLAAGARIRGSIKEVKSKLMEAVCHEIRNRH"
     gene            3583..4305
                     /gene="truB"
                     /locus_tag="VIS74_05105"
     CDS             3583..4305
                     /gene="truB"
                     /locus_tag="VIS74_05105"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEY5792654.1"
                     /translation="MKSETAIDGVLCIDKAPEMTSHDVVAITRRSLDTKKVGHCGTLD
                     PLATGLLIITIGRGTKIQDLLMSEDKEYVGTLKLGQTTDSQDAQGKITSEQPVPEFTR
                     RQIEAAFSKFHGDFYQTPPMVSAIKKDGVPLYKLARQGKEVAREPRFVHVYAHEILSV
                     RPDEIEFRVVCSKGFYVRTYAFDIGQELGCGAHLKALRRTKSGRFSGENALTVDELKN
                     LPRERVVERVLSLPEVSRLRGA"
     gene            4625..5545
                     /locus_tag="VIS74_05110"
     CDS             4625..5545
                     /locus_tag="VIS74_05110"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004159.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HEY5792655.1"
                     /translation="MLRSIAELQSVAKPIVVAIGVFDGVHLGHQALIRRAQEEAGRLG
                     AVAVVLTFDPHPVRVLRPAKAPRLLTSTPHKQRLIEKLGVNWLVLQPFDAAFAAQPPE
                     VFIQQLAASAQPLKSVCVGHQWAFGKGRAGNVELLRQAGKRLNFEVVEIPPVEIAGQM
                     VSSTRIRKAVEAGDFATAESCLGRSYTILGTVEHGAQLGGKIGFPTANLSAHSEQFPP
                     NGVYAVRALVDDHSLNGVANIGIRPTVTPDAARTLEVHLFDFREDLYGCDVEVQFETR
                     LRDEQKFDSLDALKNQIRQDCESARRVLEV"
     gene            5577..5807
                     /locus_tag="VIS74_05115"
     CDS             5577..5807
                     /locus_tag="VIS74_05115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792656.1"
                     /translation="MVKTQVQIPDRLYLEAKRIATECEMSFAEVVRRGLEKMTEEYPA
                     GRMDPREWQLPPGKDMGDALIPEEEWDTAGHE"
     gene            5809..>6140
                     /locus_tag="VIS74_05120"
     CDS             5809..>6140
                     /locus_tag="VIS74_05120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792657.1"
                     /translation="MLAFDTNIAVYAANLRDPRSARARAFLETLIANRDVAICDLMLV
                     ELYLKLCNGKIFPNPWTPAEAARICLSYRENRAWKLIEGVFEMPAVWEKAALPGFAFR
                     RIVDVRLAL"
BASE COUNT         1284 a         1973 c         1832 g         1051 t
ORIGIN      
        1 aaacggcaaa ccgatttctc atacctcatg gcagccaaaa tcaccaccgg caaaaacgaa
       61 ccttacccga gctcaagggc caaaaaaccg gccttttcgc ctaagcccgg cgctgtcccc
      121 tcgactccgg ctgcttccga aaaaccttcc gacgcccccc ccgctcccaa agctcccgtc
      181 gaagcggtct cgctgatcga gcccaagaaa aaaatccgcc gggcggatct gggggattcc
      241 gggtttaaaa aaccttttct tcccaccatt ggccccgtcg cgcccaaggt ggagcccgaa
      301 cccccgaaag ccgcggcgcc agcccccacc ccggctcccg ctcccgcacc ggtggtggaa
      361 gacctgatca gcggcaaacc caagccggct ccggtggtcg aacccaaggt ggaggaaccc
      421 gaagcggaag tcgaatcggc caaaatcatt cacatcaaac cgccgatcat tgtccgcgaa
      481 ctcgcggaag ctttgggcat caaagcgttc gaattgatcc gtgagctgat ggcgctgaat
      541 attttcccct ccatcaatca atccatcgaa ccggacatcg ccgcccggat ttgcgaaaaa
      601 tacggattca tctttgagaa ggagcgccgg gaaaagggcg cgggcatcca ccggcaggaa
      661 gtcgtcattg tggcgcccaa ggctccggaa aagcccaagg aggaggaact ttccctgcgt
      721 ccgccggtca ttaccatgat gggccacgtg gaccacggca agacctcgct gctcgacgcc
      781 atccgcaaag cccgcgtggc ggccggggag gccggcggca tcacccagca catcggcgcc
      841 tacagcgtcg agcgggatgg ccaaaagatc acttttctcg acactccggg ccacgccgcc
      901 ttcacggcca tgcgcgcccg cggcgccaat gtcacggaca tcgtcgtgct cgtggtcgcg
      961 gcggacgacg gcctcatgcc gcaatccatc gaagcgatca accacgccaa ggcggccggg
     1021 gcaaccatca tggtggccat caataagatc gacctgcctt cggcgaatcc cgaccgggtc
     1081 aagcaacagc tacaggagcg cgacctgacg ccggaagact ggggcggcac cacggtcatc
     1141 tgcccggtca gcgcgaccaa gggcaccggg gttcccgaat tgctggatat gattcatttg
     1201 caggccgagg tgctcgaact caaggccagc tcccaggcgg atccccgcgc catcgtcatc
     1261 gaagcgcagg tggaagccgg ccgcgggccg accgccacgg ccattgtcaa gatgggcacc
     1321 ctgaaggtcg gccaggcctt catctgtgga aattactggg gcaaggtcaa acagctcatc
     1381 aacgatctcg gcaagccgat caaggaggcc ggcccttcga ctcccatcaa ggtgctgggc
     1441 ttcaccggcc tgcccaacgc cggcgacgaa ctcgtcgtca tggattccga acgcagcgcg
     1501 cggatgctca gcgaggaacg cctcgacgcc ctgcgcacgg aaaagctcgc cgtcccgcag
     1561 cgcgcgaccc tggaaagcct gtttgcgacc atcgccggcg agcagaagaa aacgctgcgc
     1621 gtcgtgctca aatccgacgt gcaggggtcg atggaagcac tgcgcggcgc gctcgaggac
     1681 atcccgtccg acaaggtcga tctgaatgtc attcacgccg cggtgggtcc gatcagcgaa
     1741 tccgacgtgc tgctcgccag cgcctcgaat gccgtcatcc tcggcttcag cgtcaaggtg
     1801 gaaaacaacg cctcggccac cgccaagcgc gagggcgtgc agatcaaact ttacagcatc
     1861 atttacgaat tgctcgacca ggtgcgcgag gcgatggccg ggttgctcga tccggaactg
     1921 cgcgagaccg tcatcggcca cgccgaagtc aagcaggtgt tcgacctcac caagggcaag
     1981 gtcgcgggct gccaggtcac ggacgggcgc atcctccgca ccggccgcgc ccgcctgctg
     2041 cgccgccggc agccgatcta cgacggcggc ctcggcacgc tgcgccgctt ccaggacgac
     2101 gtcaaggaag tgcgcaacgg cctggagtgc ggcatcaagc ttggcgattt ttcggaatac
     2161 gaagtgggcg acatcatcga atgctacacc ttggacaagg tgccgcagac cttgtaaccc
     2221 gcgcctttcc ggcagcctcc atttcaggtc atgaacaacc gactggtccg cgtctgcgaa
     2281 gtgctcaaac gggagctcag cacgctcatg caaaaagcga tgagcttcga tgccccgctg
     2341 gtcagcgtcg ccgccgtgga catcacgccg gatctcaagc atgcccattt ttacatctcg
     2401 gcctttggca acgacgcgca gaagcgcgcc gcgctgcaga ccctggagca acaccgcccg
     2461 atgctgcagc acgaggtcag caaacgcgtg acgcttaaaa atacgccgca cttccacttc
     2521 cacctcgaca actcgatcga acgcggcgtg cgcgtcctgc aggtcatgga cgaactcgga
     2581 ctggcggagc cgccgcccaa gggcaaaaaa cgggactcgt gaacgccgaa aacgcgtctt
     2641 tcgcccaaat cggagcggcc atcgccgccg cgaaaaccct cctcgtaacc agccatctgc
     2701 ggcctgacgg cgacgcgctc ggctcgaccc tcgcctgcgc gctgtggctc cggcagctcg
     2761 gcaaggacgt gacggcgtgg aacgaagacg gcgtgctcga aaaattccac tatctgccgg
     2821 aatggccgct ggtcacccgc cccgagggga acccgcggga atttgacgcc gtcatcctgc
     2881 tcgacacctc ggtcaaaaac cgcgcgggca cgtgcctgga tcacatcgcg aaagccggcg
     2941 tgtggatcaa tctggatcac cacatcagca atggccggtt cggcgatctc aattacgtgg
     3001 atcccaccgc gccggcgacc ggcgaaatcc tgcaccagtt tctccaatcc caaaacgccg
     3061 cgatcacccc cgggatcgcc gcgaacctct ttgccgccat ttcgaccgac acgggctcct
     3121 tccaatacgc cggcacgtcc ccgcgcacct tccggatcgc cgcggagctg gtggaggcgg
     3181 gcgtggatgt cgcgcagctt tcgcggcgga tgtatgactc ccaaccccgg cgccggctcg
     3241 acctgctgcg ccacgcgcta aacgccgcgg aattcgcctg caacgatcac gccgtttcct
     3301 tcacgctcac gcttgccgat gccgaacggc tgggagtcgt tcccgaggac aatgaaggca
     3361 tcatcgacca gctgcgggcg gtggataccg tcgtggccgc ggtgtttttt gaggaattgc
     3421 ccgaaaaccg ggtccgcgtc agcgcgcgct ccaaggatcc ccgcgtggac gtatgccgca
     3481 tctgcgggct cttcggcgga ggcgggcacc ccctcgccgc cggcgcccgc attcgcggct
     3541 caatcaaaga agtaaaatcc aaattgatgg aggcagtctg ccatgaaatc cgaaaccgcc
     3601 attgatggcg ttctctgcat cgacaaagcg cccgagatga cctcgcacga cgtcgtggcg
     3661 atcacccgcc ggtcgctcga caccaagaaa gtcggccatt gcggcacgct ggatccgctc
     3721 gccacggggc ttctcattat caccatcggc cgcggcacga agatccagga cctcctcatg
     3781 agcgaggaca aggaatacgt cggcacgctc aagctgggcc agaccaccga cagccaggac
     3841 gcccagggaa aaatcacctc ggaacagccg gtgccggaat ttacccgcag gcaaatcgaa
     3901 gccgcgttct cgaaatttca cggggatttt taccagactc cccccatggt ctccgccatc
     3961 aagaaagacg gcgtgccgct ttacaagctg gcgcggcagg gcaaggaagt cgcgcgggag
     4021 ccgcgtttcg tccacgtgta tgcgcacgag attttgtccg tgcggcccga cgaaatcgaa
     4081 ttccgcgtgg tttgcagcaa gggtttttac gttcggacct acgcgttcga catcggccag
     4141 gaactcggct gcggggcgca cctcaaggcg ctgcgccgga ccaaatccgg gcgtttttcg
     4201 ggggagaatg ccctcacggt ggacgaattg aaaaatctcc cgcgagagcg ggtcgtcgag
     4261 cgggttttga gcctgccgga ggtctcgcgc ctgcggggag cgtgaggaag ttttaagaaa
     4321 atggggattt gcgaactcgg cgctcttcaa acttgccaac ttaagactta gagcgattct
     4381 caaaagagat ttccgaaatg cgattcactg taggggcagc cggagcgaag cgaagacagc
     4441 ctgtcccgcg gaagcgggac agcccgaggg gcgcacgaag tgcggcaatt gtgccaaccg
     4501 cccagcgtaa acggggatgc ggggttcccg tttccccgca aacaaaggcg cttgacacaa
     4561 gcgcctctac aacccgtcgg gtgtttcgga aatctctttt gagaaatact ataagacttt
     4621 gaaggtgctt cgttccatcg ccgaacttca gtccgtggcc aagcccatcg tggtcgctat
     4681 cggggtgttt gacggcgtgc atctcggcca tcaggcattg atccggcgcg cccaggaaga
     4741 ggccggacgg ttgggagcgg tcgcggtggt gctgacgttc gacccgcatc ccgtccgggt
     4801 tttgcggccg gccaaggcgc cgcgcctgct gaccagcacc ccgcacaaac agcggctcat
     4861 cgaaaaactg ggagtcaact ggctcgtgtt gcagccgttc gatgccgcgt ttgcggccca
     4921 gccgccggag gttttcatcc agcagcttgc cgccagcgcc cagccgttaa aatccgtctg
     4981 cgtcggccat caatgggcat tcggcaaggg acgcgcgggc aatgtggaac tgctccgcca
     5041 ggccggcaag cggctgaatt ttgaagtggt cgaaattccg ccggtggaaa tcgcggggca
     5101 aatggtcagc agcacccgca ttcgcaaggc cgtcgaagcc ggcgacttcg ccacggcgga
     5161 gagctgcctg ggccgctcct acacgattct cggcaccgtc gaacacggcg cgcaactcgg
     5221 cgggaagatc gggtttccga ccgccaatct gagcgcccac agcgagcagt tcccgccgaa
     5281 tggcgtttac gcggtgcggg cgctcgtcga tgaccattcc cttaacggcg tggccaatat
     5341 cggcatccgc cccaccgtca ccccggacgc cgcccgcacg ctggaggtcc atttgttcga
     5401 ctttcgcgag gatctttatg gctgcgacgt ggaggttcaa tttgaaaccc ggctgcgcga
     5461 tgagcaaaaa ttcgattcgc tcgacgcgct caagaatcaa atccggcagg attgcgaaag
     5521 cgcccggcgc gtgctggaag tttgacttta attcgccatg atggcaaaat gccatcatgg
     5581 taaaaaccca ggtgcaaatt ccggatcgcc tttatcttga ggccaagcgg atcgcgacag
     5641 aatgcgagat gagctttgcg gaggtggtgc gccggggcct ggaaaaaatg accgaggaat
     5701 atcccgccgg acggatggat ccgcgcgagt ggcagctccc gccgggcaaa gatatgggtg
     5761 acgccttgat tcccgaggag gaatgggata cggcgggtca cgaatagaat gctggccttc
     5821 gataccaaca tcgcggttta cgcggccaat ctccgcgacc cccgatcggc cagagcgcgg
     5881 gcgtttctgg aaaccttgat cgccaatcgc gacgtggcga tctgcgattt gatgctggtt
     5941 gaactctatc tgaaattgtg caacggcaaa attttcccaa acccatggac tccggcggaa
     6001 gccgcccgca tttgcctttc ctatcgggaa aatcgggcct ggaaattaat cgagggcgtt
     6061 tttgaaatgc cggccgtttg ggaaaaagcc gctttgccgg gattcgcctt tcggcggatc
     6121 gtggatgttc gcctggcgct
//
LOCUS       DATDAY010000257         2889 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_1730994, whole genome shotgun sequence.
ACCESSION   DATDAY010000257 DATDAY010000000
VERSION     DATDAY010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2889)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2889)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2889
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1730994"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1006
                     /locus_tag="VIS74_05125"
     CDS             <1..1006
                     /locus_tag="VIS74_05125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792658.1"
                     /translation="EDDPIAQSASLLFDRIVRQGRAFGIHVLLGSQTLGGAYTLARAT
                     LGQMVIRVALQCNEADAYLIMDESNPAPRLLSRPGEGIYNDAAGALEGNSPFQVVWLS
                     DEERDAQLEVVRELAEARGFRDAAPIVFEGNSPADIRENIPLRRALDGESAPARGVAR
                     CWLGAPNAIKGPTEAVFRPQSGSHLLIVGQREETIQTMLGVSLLALAAQQPKARLILF
                     HAADASGSDFLSEAARLVPGGITLVGNPRVAEVIGELSAELQARSAAGGRAGIFRRSS
                     CSFTGCPNSRSCGRRTNSTFPGRGREPGRNSPRCCRKADPTASMWSSPPTASTTSAAS
                     "
     gene            961..1260
                     /locus_tag="VIS74_05130"
     CDS             961..1260
                     /locus_tag="VIS74_05130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792659.1"
                     /translation="MVITADSFNNVGRFLSRKSLGEFEMRVVFQMSANDSASLIDSPQ
                     ASGLGLHRALLANEQEGSLETFRPYARPDRGWLEEAGGKLRGRGNLPIDFPAHSA"
     gene            1273..2463
                     /locus_tag="VIS74_05135"
     CDS             1273..2463
                     /locus_tag="VIS74_05135"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY5792660.1"
                     /translation="MPNRSLQSAAIFFTIFIDILGFGIVIPVLPLYAEHFGANAWQIG
                     WLVGVFSMAQFFFAPLWGKISDRIGRKPVLLIGILGTIAGYLWMGLAHTLWMLFAARL
                     IDGIAGANISAAQAYMADISSAENRARAMGLIGAAFGLGFVFGPALGGWASQTFNYAA
                     PMFIAAALAFLNFLFVVFWLPESRPPGLIGEKGEPLFPGLLSHVSRRIYFWSLGSYFL
                     VIFGFSMMTTVFALLLLHRFELDAMHTGYLFAGIGILGAFIQGGLIGRLVRRFGEARL
                     AFFGCLSMGAGLAGLALSPDLKGLLLSSAVVGIGNSLLMPTLSALASRSADEQWQGRA
                     LGVLQSSGSLARWLGPLLAGALLGWPLAGGMGQYAFLPLIGAAAILGAAAITALQLAG
                     KPAK"
     gene            complement(2493..>2889)
                     /locus_tag="VIS74_05140"
     CDS             complement(2493..>2889)
                     /locus_tag="VIS74_05140"
                     /inference="COORDINATES: protein motif:HMM:NF014021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HEY5792661.1"
                     /translation="SMTQTRPPGMARIMLTIMPGGGEIMKVEDHIPIAYAHRLASKVA
                     RYLKKRYGATKVMLYGSLVMGCYNPDFSDIDVYFEGVREEWVADALADCRLNFGEQDI
                     SGRKRIDYTAASSLPKEMQAYIRQRAEEL"
BASE COUNT          445 a          910 c          946 g          588 t
ORIGIN      
        1 cgaggacgat ccgatcgccc agagcgcctc gctgcttttc gaccggatcg tccgccaggg
       61 gcgggcgttc gggattcacg tgctgctcgg ttcccagacg ctcggcggcg cctacacgct
      121 cgcccgggcg acgctcggcc agatggtcat ccgcgtcgcc ctgcaatgca acgaggcgga
      181 tgcctacctc atcatggacg agtccaaccc ggccccgcgg ctgctttcgc ggcccggcga
      241 aggcatttac aacgatgccg ccggcgcgct ggagggcaac agcccgttcc aggtcgtctg
      301 gctttccgac gaggagcggg atgcccagct ggaggtggtg cgggaactgg ccgaagcccg
      361 gggcttccgc gatgccgccc cgattgtctt cgagggcaat tcgccggccg acatccggga
      421 aaatatcccg ctgcgccgcg ccctggacgg cgaatcggcc ccggcgcgcg gggtggcccg
      481 ctgctggctg ggggcgccca acgccatcaa gggacccacc gaagccgtct tccgcccgca
      541 gagcggcagc catctgctca tcgtggggca gcgggaggaa acgattcaga cgatgctggg
      601 agtttcgctg ctcgcgctcg cggcgcagca gccgaaggcc cggctcatcc tctttcacgc
      661 cgccgatgcc tccggttcgg attttctttc cgaagccgcc cggctcgtgc ccggcgggat
      721 caccctggtg ggaaatccgc gggtcgcgga agtcatcggg gaactttccg ccgaactgca
      781 ggcccggagc gcggcggggg ggcgggcggg aattttccgc cggtcttcgt gttcattcac
      841 gggctgccca aattcaagaa gctgcgggcg gaggacgaat tcgacttttc cggggcgggg
      901 ccgggagccg gggcgcaact cgccacgctg ctgtcggaag gcggatccca cggcatccat
      961 gtggtcatca ccgccgacag cttcaacaac gtcggccgct tcctgagccg gaaatcgctg
     1021 ggggaattcg agatgcgggt cgtcttccag atgagcgcca atgactcggc gagcctcatc
     1081 gattcaccgc aggccagcgg cctcggcctg caccgggcgc tgctcgccaa cgagcaggag
     1141 ggatcgctgg aaaccttccg gccgtatgcg cggccggacc gcggctggct ggaggaagcg
     1201 ggcggaaagc tgcggggccg cggcaatttg cccattgatt ttcccgcgca ctccgcttga
     1261 gtcatcgttt ccgtgccgaa tcgatccctg caatccgccg ccattttttt cacgatcttt
     1321 atcgatatcc tcgggttcgg aatcgtgatt cccgtcctgc cgctctacgc cgagcatttc
     1381 ggagcgaacg cctggcagat cggctggctg gtcggcgttt tttcgatggc gcaatttttc
     1441 ttcgctcccc tctgggggaa aatctccgac cgcatcggcc gcaagccggt tctgctgatc
     1501 ggaatcctcg gcacgatcgc gggatatctc tggatgggcc tggcccacac gttgtggatg
     1561 ttgtttgcgg cgcggctcat tgatggcatt gccggggcga acatcagcgc ggcgcaggcc
     1621 tacatggccg acatctccag cgcggaaaac cgcgccaggg cgatggggct gatcggcgcc
     1681 gcgttcggcc tgggcttcgt gttcgggccg gcgctcggcg gatgggccag tcaaactttc
     1741 aactacgcgg cgccgatgtt catcgccgcg gccctcgcct ttctgaattt tctgttcgtc
     1801 gttttctggc tgcccgagag ccgcccgccc gggttgattg gcgaaaaagg ggaaccgctt
     1861 tttccgggtt tgttatcgca cgtttcccgg cggatctatt tctggagcct gggcagttat
     1921 tttttggtga tttttggatt ttccatgatg accaccgttt tcgcgctgct cttgctgcat
     1981 cgcttcgagc tggatgccat gcacacgggc tatctgttcg ccggcatcgg gattctgggc
     2041 gcctttatcc agggcgggtt gatcggccgg ctggtgcgcc gtttcggcga ggcccggctg
     2101 gcgttctttg gctgcctgtc gatgggagcc ggcctggccg ggctggcgct gagtcccgat
     2161 ttgaaggggc tgctgctttc cagcgccgtc gtcggaatag gcaacagcct cttaatgccc
     2221 acgctgtcgg ccctggcctc ccgcagcgcg gatgagcagt ggcagggccg ggcgctcggg
     2281 gtgctccaat ccagcggcag cctggcgcgc tggctggggc cgctgctggc cggcgcgctg
     2341 ctggggtggc cgctggcggg aggcatggga caatacgcct tcctgccgct gatcggagcg
     2401 gcggcgatct tgggagccgc cgcgattacg gcactgcagc tcgcgggaaa acccgccaag
     2461 tgagcgaagc ctgacgttcg cgagttttcg tttcagagtt cctcggcgcg ttgccggatg
     2521 taggcctgca tttccttggg cagcgaagaa gccgcagtgt aatcgatgcg cttccgcccg
     2581 gatatgtctt gctcgccaaa attcagccgg caatcagcca aggcgtcggc cacccattcc
     2641 tctcgaactc cttcaaaata aacgtcaatg tccgagaaat cgggattgta acatcccatc
     2701 accaacgagc cataaagcat gaccttcgtg gcgccgtagc gttttttcag gtagcgcgcg
     2761 accttggaag caagccggtg cgcgtaggcg atcggtatat gatcctccac tttcatgatt
     2821 tcacctcccc ccggcataat tgtaagcata atccgggcca ttcccggagg gcgggtttgg
     2881 gtcattgac
//
LOCUS       DATDAY010000258         2274 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_865821, whole genome shotgun sequence.
ACCESSION   DATDAY010000258 DATDAY010000000
VERSION     DATDAY010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2274)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2274)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2274
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_865821"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..347)
                     /locus_tag="VIS74_05145"
     CDS             complement(<1..347)
                     /locus_tag="VIS74_05145"
                     /inference="COORDINATES: protein motif:HMM:NF020397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HEY5792662.1"
                     /translation="MASKAKFTTMEAYIASFPKEIQGILEQIRQTIRKAAPGAVEAIS
                     YQIPTFKLNGSSLVHFAAWKDHIGFYATPSGNAAFQKELAQYKVAKGSIQFPLDEPIP
                     YDLVAKIAKFRVK"
     gene            624..1115
                     /gene="rpsF"
                     /locus_tag="VIS74_05150"
     CDS             624..1115
                     /gene="rpsF"
                     /locus_tag="VIS74_05150"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEY5792663.1"
                     /translation="MALYEHVFMARQDASPAQVEALVEQFKGVITAGGGKVGKVESWG
                     IRSISFRVKKNRKAHYTLMNIDAPYAAVAEMERLMLISEDVIRFMTVAVDEHEEGQSA
                     MLRRRDEREERGSFGDDRGFGFGGGGGGGFGGGGGFGGRGGAGGGRGRPREDRVEPVA
                     EVL"
     gene            1121..1357
                     /gene="rpsR"
                     /locus_tag="VIS74_05155"
     CDS             1121..1357
                     /gene="rpsR"
                     /locus_tag="VIS74_05155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007403456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEY5792664.1"
                     /translation="MEQTARRPFFRRRKSCPFTGEGAPKIDYKDVRLLQRYISERGKI
                     VPSRISAVSAKKQRKLAQAIKRARFLGLLPYVLQ"
     gene            1370..1951
                     /gene="rplI"
                     /locus_tag="VIS74_05160"
     CDS             1370..1951
                     /gene="rplI"
                     /locus_tag="VIS74_05160"
                     /inference="COORDINATES: protein motif:HMM:TIGR00158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEY5792665.1"
                     /translation="MEIILLERVAKLGALGQVVKVKDGFARNFLLRRGKALRATEANR
                     AKFETQRAVLEAKNAERRKGAQDEAKGLDGKSFVIIRQAGESGQLYGSVSPRDIAEAA
                     STSGVAVERSHVLLDTPIKTIGLYTVTVAPHPEVEVKVTINVARSADEAAAQQRGENL
                     AGPVSDRAEIRAAAEALFDEEAAIPEELATPAS"
BASE COUNT          456 a          725 c          647 g          446 t
ORIGIN      
        1 tccttcaccc ggaacttcgc gattttcgcc accaggtcat agggaatggg ctcatcaagc
       61 gggaactgga tggacccctt cgccaccttg tactgcgcca gctccttctg gaacgccgcg
      121 ttgccggacg gcgtcgcata aaacccgata tgatccttcc acgcggcgaa gtgaacaagg
      181 ctgcttccgt tcaacttgaa cgtcggaatc tgatagctga tagcctcaac cgcccccggc
      241 gccgccttcc ggattgtctg cctaatctgt tcaagaatgc cctgaatctc tttcggaaaa
      301 gaagcgatat aggcttccat cgtcgtgaat tttgctttgc tggccatggc gtattattag
      361 cccataaccg gtttggttaa agcccttccc cgccgccaat cccgcccttg caatccagcg
      421 ccgtttccgc catacacccc ccaacttcga ccacccagcc gggggctgaa cggaaggtct
      481 ggcttgccag atagggcgaa aaatcccgtc ttcccgtgtc tccgctctta cgtttgaccg
      541 gtctttcgaa cctttccctg gttcgtcaga ggctctgcgc tggatgccga aagtagaacg
      601 agaaacagaa aggttatcag aacatggctc tgtatgagca cgtattcatg gcccgtcagg
      661 acgcctcgcc ggcccaggtt gaagccctgg tcgagcaatt caagggcgtc atcacggcag
      721 gcggcggcaa ggtcggcaag gttgaatcct ggggcatccg ctccatctcc ttccgcgtga
      781 agaaaaaccg caaggcccac tacaccctta tgaacatcga tgcgccctat gcggctgttg
      841 ccgaaatgga acgcctcatg ctgatcagcg aagacgtcat ccgcttcatg acggttgccg
      901 tggacgaaca cgaagaaggc cagtcggcca tgctgcgccg gcgcgatgag cgcgaagagc
      961 gcggcagctt cggcgatgac cgcggcttcg gcttcggtgg tggcggaggc ggcggatttg
     1021 gcggcggcgg tggctttggt ggccgcggcg gtgccggcgg cggccgtggc cgtccccgtg
     1081 aagatcgtgt cgaaccagtg gcggaggtgc tctaatggct atggaacaaa ctgcccgccg
     1141 cccgtttttc cggcgccgga aaagctgccc cttcaccggt gaaggcgcgc ccaagattga
     1201 ttacaaggat gtgcgcctgc tgcagcgcta catttccgag cgtggcaaga tcgtgccaag
     1261 ccgcatctcg gcggtctccg ccaagaagca gcgcaagctc gcccaggcca tcaagcgcgc
     1321 ccgcttcctg ggcctgctgc cctacgtctt gcaataggag aattgaatca tggaaatcat
     1381 tcttcttgag cgcgtcgcca agcttggtgc tttgggccag gtcgtaaagg taaaggacgg
     1441 gttcgcccgt aacttcctgc tgcgccgtgg caaggcgctg cgcgccaccg aagccaaccg
     1501 ggcaaagttc gaaacccagc gcgccgtgct ggaagcaaaa aatgccgagc gccgcaaggg
     1561 cgcccaggat gaggccaagg gccttgatgg caagtccttc gtcatcatcc gccaggccgg
     1621 tgaatcgggc cagctctacg gctctgtttc gccccgtgat atcgccgaag ccgcatcgac
     1681 ctccggtgtg gccgtcgaac gcagccacgt gctgctcgat acgccgatca agaccatcgg
     1741 cctgtacacg gtaacggttg ccccgcatcc ggaagtggaa gtgaaggtta ccatcaacgt
     1801 tgcccgctcg gccgatgaag ccgcagccca gcagcgcggt gagaacctcg caggccccgt
     1861 ctccgaccgc gccgaaatcc gcgccgcagc cgaagccctc ttcgacgagg aagccgccat
     1921 ccccgaggaa ctggcaactc cggcaagcta agcggctgac ttcatttaaa attgggccgc
     1981 ctgcgtgggc ggcccttttg tttgagggtg agtcaaccca aacttttgca cagccctgtg
     2041 tataacgcgt ataatttgat tcagaatcaa ttcatccccg ggaaactgct acccttcagc
     2101 catggccact caacccaatc tccgtcccgt cgataacgac ggtccagacg ataccttccg
     2161 caccccgccc cacaaccttg aggcggagca ggcgctgctt ggcgccatcc tggtgaacaa
     2221 cgaggcctgc gaccgcgtct cgtcgttcct gctgcccgat catttctctg aagc
//
LOCUS       DATDAY010000259         2107 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_747981, whole genome shotgun sequence.
ACCESSION   DATDAY010000259 DATDAY010000000
VERSION     DATDAY010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2107
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_747981"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(97..510)
                     /locus_tag="VIS74_05165"
     CDS             complement(97..510)
                     /locus_tag="VIS74_05165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792666.1"
                     /translation="MTFTISFGDYEICEFCGKEQIRFVHYLQHPDWKDTIAVGRICAS
                     DLSGDPKADSAERRLRNLAKRRANFLKLKSWKTSAKGNVWIEYQDHHIIVIKCRNGKF
                     TLRIDGEAGKLFFPDRHTAMLRAFDVVMRKIRRER"
     gene            668..>2107
                     /locus_tag="VIS74_05170"
     CDS             668..>2107
                     /locus_tag="VIS74_05170"
                     /inference="COORDINATES: protein motif:HMM:NF023149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malectin domain-containing carbohydrate-binding
                     protein"
                     /protein_id="HEY5792667.1"
                     /translation="MKLVSGGGGGSGGSNSSVHYFYGLGSTGNGRSGGRLLIESEKPS
                     AALYNPSGIVITAGQSVRSYLVDGVLSQALSDSQLTTVNVTDATHYEIRSYHIGQVTN
                     ASSAAGPWTVSNPGGYFKKTIVENPGPIPIADTEDDGLYHTERTGQSFGYELPVVTGD
                     YLVTLHFCENFQDGAGARKFDVSAEGAVVLDGYDIFAAAGGANIARRETFPVTVGDGA
                     LNLGFATVLYQAEVSGIEVSKAPVAEIANTDDDALYQTERTGDFACDLALPNGDYAVV
                     LEFAERYYDAAGARVFNVAEMNLSNPQNPALLLDHFDIRAAAGARDTAVTRTLPVAVT
                     DGQLNLRFAGVTRMPRLAALRVYQVVNGQPASLVAAINAGGGAFTAADGTAYSADAGF
                     TGGAAESAGGGDLVAIHAGGEELTAADGTVFASDTGYSGGKSLFSGGTGVRFTEMESG
                     AVKQVYDFSWAVDPLSGKFGDVTMASGAVS"
BASE COUNT          417 a          533 c          754 g          403 t
ORIGIN      
        1 gcgggatatt gcaccgaggg gctcctacga aaacgagtga gattcgcgac ttgcgcgtgc
       61 tatgtggggt tattgatggg aagcccagag caaaagtcaa cgttcacggc ggatttttcg
      121 catgacgaca tcgaaggcgc gaagcatggc ggtgtgcctg tcggggaaga aaagttttcc
      181 agcctctcca tcaatgcgaa gagtgaattt gccgttgcgg cacttgatga caatgatgtg
      241 atggtcctga tactcgatcc agacattgcc cttggcggaa gtcttccagc ttttgagctt
      301 gaggaagttg gcacgtcgct tggcgaggtt gcgcaggcgg cgctcggcgg aatcggcctt
      361 gggatcaccc gaaaggtcgc tggcgcaaat gcggcccacg gcaatggtat ccttccaatc
      421 ggggtgttga aggtaatgga cgaagcgaat ctgctccttg ccgcaaaatt cgcagatttc
      481 gtagtcaccg aagctgatcg tgaacgtcaa cggcgcgaag ccattgccag cccgatttgg
      541 ggcaccccgg agtgcttttc agaaggtgat ccattaagga aaaagtgtag cggaggagga
      601 gaaaaagcgc cttccttttt gaaagaaatg gggcagcagc gaaggccagg aggcggaccg
      661 caaaactgtg aagctggttt ccggcggagg cggaggcagc ggcggatcga acagcagtgt
      721 ccactatttc tacggcttgg gttccacggg caatgggcgg tccggggggc ggttgctcat
      781 cgagtcggaa aaaccctcgg cggcgcttta caacccctcg ggcattgtga ttacggcggg
      841 ccagagtgtc cggagttatc ttgtggatgg cgtgctttcg caggcattat cggactcgca
      901 gttgacgacc gtcaacgtga cggacgccac gcattacgaa atccgcagct accacatcgg
      961 ccaggtaacg aatgcaagca gcgcggctgg gccgtggacg gtttcgaacc ccggagggta
     1021 tttcaagaag actatcgtgg aaaatcccgg tccgataccg attgccgata cggaggacga
     1081 tgggctgtat cacaccgagc ggaccggcca gagctttgga tacgagctgc ccgtggtgac
     1141 gggggattat ttggtcacgc tgcacttctg cgaaaatttc caggacggcg cgggcgcgcg
     1201 gaagttcgac gtgagcgccg agggggcggt cgtcctggac ggctacgata ttttcgccgc
     1261 cgccggcggg gccaatatcg cgcggaggga aacgtttccg gtaacggtgg gagatggggc
     1321 gctcaatctg ggtttcgcaa ccgtgctgta tcaggcggag gtgtcgggaa tcgaggtgtc
     1381 gaaggcgccg gtggccgaga tcgcgaacac cgatgacgac gccctgtatc agaccgagcg
     1441 cacgggcgac tttgcctgcg acctcgcctt gcccaacggg gattatgcgg tggtgctgga
     1501 atttgccgag cgttattacg atgcggccgg ggcgcgggtg ttcaatgtgg ccgagatgaa
     1561 tctcagcaac ccccagaatc cggctctgct gcttgatcat ttcgatatcc gcgcggcggc
     1621 gggcgcccgg gacacggcgg tgacccgcac gctgcccgtg gcggtgaccg acgggcagtt
     1681 gaacctgaga tttgcgggag tcacgcgcat gccgcggctg gcggctctgc gggtgtatca
     1741 ggtcgtgaac ggacagcccg cgtcgctggt ggcggcgatc aacgccggag gcggcgcgtt
     1801 caccgcggcg gacgggacgg cgtattcggc cgacgcggga tttacgggag gagccgccga
     1861 gagcgcggga ggcggggatc tggtggcgat ccatgccgga ggagaggaat tgacggctgc
     1921 cgatgggacg gtctttgcga gcgatacggg ctattccggc ggcaagagcc tgttctcggg
     1981 aggcaccggc gtgcggttta ccgagatgga aagcggggcg gtgaagcagg tgtatgattt
     2041 ttcgtgggcg gtggatccgc tgagcggaaa gttcggggat gtgacgatgg cgagcggcgc
     2101 ggtgagc
//
LOCUS       DATDAY010000260         2247 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_1342021, whole genome shotgun sequence.
ACCESSION   DATDAY010000260 DATDAY010000000
VERSION     DATDAY010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2247)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2247)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2247
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1342021"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            137..724
                     /locus_tag="VIS74_05175"
     CDS             137..724
                     /locus_tag="VIS74_05175"
                     /inference="COORDINATES: protein motif:HMM:NF014471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HEY5792668.1"
                     /translation="MSEEAVGEAPAKPNPTLLELALCFNQIAIASFGGGLSAWSRQVL
                     VEDKKWMEDEEFLSASTLCRVLPGANQVNLAVFVGSRFRGLTGAGAAVVGLTLIPMVI
                     VIGLGWFYFTYSQVPALKSILNGISPVAVALSAAMAFKAGKKCLNGVMPVAFAVAMFV
                     LSTFWRLPLLESLAVLGPLAVWWAWPRDKKTKEAA"
     gene            721..1269
                     /locus_tag="VIS74_05180"
     CDS             721..1269
                     /locus_tag="VIS74_05180"
                     /inference="COORDINATES: protein motif:HMM:NF014471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HEY5792669.1"
                     /translation="MSPLTLTRLLLLFGSLSVLSIGGGNSIIPEMHLQAVKGYHWMTD
                     RQFADVFAIAQSAPGPSVLIVTLIGYKAGLPGGIGWAVFGAVAATIAMIVPAGLLVYW
                     VAQFWQKAEKSKWRHAVEKGFAPLAVGLILATAFIVSRSADHSKTAVALSFVCTLIFA
                     TTKINPLIIVAIGGLIGWLGWV"
     gene            1373..>2247
                     /locus_tag="VIS74_05185"
     CDS             1373..>2247
                     /locus_tag="VIS74_05185"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF014803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEY5792670.1"
                     /translation="MHQFSYRDNQLYCEGASLDQIADCVGTPAYVYSANTIRENYRRL
                     HAALAPLDHLVCYAMKANSNLSVLKLLAGEGAGFDIVSGGELFRAIKSGGFTGHCTFA
                     GVGKTVAEIEYALKVGIYSFNVESEEELERINHVAARHNLTAPVAVRVNPDVDAQTHK
                     YISTGKSKNKFGIGLDRAAAVYERAAALPNLQIRGVQTHIGSQILDPQPFADAVKKLL
                     PLVEELKAKYGIEFFSIGGGIGIVYDPALESGATAWWDRAAEQRRITPEAYARLLVPL
                     LQPLDLKILLEPGRF"
BASE COUNT          409 a          706 c          703 g          429 t
ORIGIN      
        1 aatgaacagg tgatccgcga gctcaacgag gtgtttgaaa gcgattggga gagctcgcac
       61 cgctacgagg cgccggatcc gctggtggcg accgagcacc acgaggggga ttttccgcac
      121 gacaaagagc tgacgcatga gtgaggaagc cgtgggggaa gctcccgcca agcccaaccc
      181 cacgctgctg gagctcgcgc tctgcttcaa ccagatcgcc atcgccagct ttggcggcgg
      241 gctgtcggcc tggtcgcgcc aggtgctggt cgaggataaa aaatggatgg aggacgagga
      301 attcctcagc gcctccacgc tctgccgcgt gctgccggga gcgaaccagg tcaatctcgc
      361 cgtcttcgtg ggctcgcgct tccgcggcct gaccggcgcg ggggcggcgg tggtcggcct
      421 gaccctcatt ccgatggtga tcgtcatcgg gctgggctgg ttttatttca cctattcgca
      481 ggtgcccgcg ctcaaatcca tcctcaacgg catctcgccc gtcgccgtgg cgctctcggc
      541 ggcgatggcg ttcaaggccg gcaaaaagtg cctgaatggc gtcatgccgg tggctttcgc
      601 cgtggcgatg ttcgtgctga gcacgttctg gcggctgccg ctgctcgaat cgctggcggt
      661 tctcggcccg ctggcggtgt ggtgggcctg gccgcgcgac aaaaaaacga aggaggcggc
      721 atgagcccgc tgaccttgac gcggctgctc ctgctcttcg gctcgctctc ggttctgtcc
      781 atcggcggcg ggaattccat catcccggaa atgcatctgc aggcggtgaa gggttaccat
      841 tggatgaccg accggcaatt cgccgacgtg tttgccatcg cgcaatccgc gcccgggccc
      901 agcgtattga tcgtcacgct catcggctac aaggcgggcc tgcccggcgg gatcggctgg
      961 gcggttttcg gcgcggtggc ggcgaccatc gccatgatcg tgccggcggg cctgctggtt
     1021 tactgggtgg cgcagttctg gcagaaggcg gaaaaatcca agtggcggca cgcggtcgag
     1081 aaaggcttcg cgccgctggc cgtggggctg atcctggcga cggccttcat cgtttcgcgc
     1141 tcggcggatc acagcaagac ggcggtcgcg ctgagcttcg tctgcacgct cattttcgcc
     1201 acgaccaaaa tcaatccgct catcatcgtc gccatcggag ggctgatcgg ctggctgggc
     1261 tgggtgtagg attttttgac agaattcaca aaattaacgg aatcggaaaa aaccagttct
     1321 caaatccccc cggaattttg ttaattctgt aaattctgtc ttcatttttc ccgtgcatca
     1381 attctcctac cgcgacaacc agctttattg cgaaggcgcg agcctcgatc aaatcgccga
     1441 ctgtgtcggc acgccggcct atgtgtattc cgcgaacacc attcgcgaga actaccgccg
     1501 cctccacgcc gcgctcgcgc cgctcgacca cctcgtctgc tacgcgatga aggcgaattc
     1561 gaatctctcc gtcctcaaac tgctcgccgg cgaaggcgcg ggtttcgaca tcgtttcggg
     1621 cggcgaattg ttccgcgcca tcaagtccgg cgggtttacc ggccattgca cgtttgccgg
     1681 ggtcggcaag acggtggcgg aaatcgaata cgccctcaag gtcggcatct acagcttcaa
     1741 cgtggaatcc gaggaggagc tggaacgcat caaccacgtg gccgcgagac acaacctcac
     1801 cgctccggtg gccgtccgcg tgaatcccga cgtggacgcg cagacgcaca aatacatctc
     1861 gaccggaaag agcaaaaaca agttcggcat cggcctggat cgcgccgcgg cggtttacga
     1921 gcgcgcggcg gcattgccca atctacagat ccgcggcgtg cagacgcaca tcggctcgca
     1981 gattctcgac ccgcagccgt tcgccgacgc ggtgaaaaaa ctcctgccgc tcgtggagga
     2041 gctgaaggcc aaatacggca tcgaattttt cagcatcggc ggaggcatcg gcatcgttta
     2101 cgaccccgcg ctggaaagcg gcgccacggc ctggtgggac cgcgccgcgg agcagaggcg
     2161 catcactccg gaagcctacg cccgcctgct cgtgcccctg ctccagccgc tggacctgaa
     2221 gatcctgctc gaacccggcc gcttcat
//
LOCUS       DATDAY010000261         2559 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_1423979, whole genome shotgun sequence.
ACCESSION   DATDAY010000261 DATDAY010000000
VERSION     DATDAY010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2559)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2559)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2559
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1423979"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..608)
                     /locus_tag="VIS74_05190"
     CDS             complement(<1..608)
                     /locus_tag="VIS74_05190"
                     /inference="COORDINATES: protein motif:HMM:NF020881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1980 domain-containing protein"
                     /protein_id="HEY5792671.1"
                     /translation="MTILFRRFATSGILLVWGVALTFFSVSGRVRDYLHPNFFLPLLI
                     CGIALVLLAVGMLVVSAEADCGDPQCCGAITNERIRFRTILAWGLLVIPLLVAVKGSP
                     NQFGASAVWNRGLVVDASALPAAAPSQPYADPPLPGTEAAQPSGAAMDSSTYLVKNER
                     GQIKVEAIDLLFAAQEEGLRPDFENKEVEIIGQFLPAKTNNAR"
     gene            complement(605..1537)
                     /locus_tag="VIS74_05195"
     CDS             complement(605..1537)
                     /locus_tag="VIS74_05195"
                     /inference="COORDINATES: protein motif:HMM:NF015715.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HEY5792672.1"
                     /translation="MPGWFNFSFPDFCYSFLSVLLEGVPFILLGTAISGLIDQFLPAR
                     LMSRLLPRNPFLGACVSGLLGMIFPMCECAVVPVIRRLIAKGLPLSHAVTYMLAAPIV
                     NPIVALSTYAAFRGQGAGEMTAFRLGLGFAVAVLAGLAVHHLAPRSVLRPRVLESALA
                     FGASSEMAPAPLLGMKARLSAALRVTVGDFLDVMLYFIIGAAIAAVFNTSVNQDALLP
                     LALNPWLATGSMMGLALMLSLCSTSDAFIAATFGLFPWAARLAFLVFGPMMDLKLLFI
                     YSSVFRPRFVIGLAIGLFILIGLLCIRLGGVLNL"
     gene            complement(1681..2418)
                     /locus_tag="VIS74_05200"
     CDS             complement(1681..2418)
                     /locus_tag="VIS74_05200"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: protein motif:HMM:NF002537.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HEY5792673.1"
                     /translation="MSLSLDHISAEEIHSLSQEFEEAPLERILEWGWKRFGARAAIGT
                     SFQGAGLVIIHTAQTNGWNFPIFTLDTGLLFPETLDLKRKLEERYQLEIESLRPEQTV
                     EEQAAEHGPELWKRRPDFCCTLRKVIPLQKKLSELDVWITGLRRQQSEGREKTRILEL
                     YKFDVLRDHYILKMNPMANWTRERVQDFLKANSIPTNALAERGYRSIGCQPCTRPASE
                     GDNERAGRWTGFDKTECGIHTFLGTNI"
BASE COUNT          579 a          813 c          740 g          427 t
ORIGIN      
        1 cgggcattat tggttttcgc cggcagaaac tggccgatga tctccacctc cttgttttca
       61 aaatccggac gcagcccctc ctcctgggcc gcgaagagca gatcgatggc ttcgactttg
      121 atctggcccc tttcattttt cacgagatag gtgctggagt ccatggccgc gccggagggc
      181 tgggcggcct ccgtgccggg aagcggcggg tcggcgtagg gctggctggg agcggcggcc
      241 ggaagggcgg acgcatccac caccaggccg cgattccaca cggcggacgc gccgaattga
      301 ttcggcgatc ccttcacggc cacgagcagg ggaatcacca gcaatcccca ggccagaatc
      361 gtccggaatc gaatgcgctc attcgtgatc gcgccgcagc attgcggatc gccgcaatcc
      421 gcctccgcgg acaccacgag catgccaacc gccaggagaa ccagcgcgat cccgcaaatc
      481 agcagcggga ggaaaaaatt cggatgcagg taatccctca cccggccgct caccgaaaag
      541 aacgtcagcg ccacgcccca gacgagcaga attcccgaag tggcaaaccg gcggaagaga
      601 atggtcataa atttagcacg cctcccaggc gaatacaaag caggccgatc aaaataaaca
      661 agccgatggc cagcccgatg acaaatcgcg gacggaacac cgagctgtaa ataaacagca
      721 gcttgagatc catcatgggt ccaaagacca gaaacgccag ccgcgcggcc cacggaaaca
      781 ggccgaaggt ggcggcgata aacgcgtccg aagtgctgca gagggagagc atcagggcca
      841 gccccatcat tgaccccgtc gccagccagg gattcaacgc caggggcagc agcgcgtcct
      901 gattcacgct cgtgttaaag accgcggcaa tggctgcgcc gatgataaaa tagagcatga
      961 cgtcgagaaa atccccgacc gtcacgcgca gggccgcgct cagccgcgcc ttcattccca
     1021 aaagcggcgc cggggccatt tccgacgagg cgccgaaggc cagcgcggat tccagcaccc
     1081 gggggcgcag cacggatcgc ggggcgagat gatgcaccgc caggccggcg aggaccgcca
     1141 cggcaaaacc caatcccagc cggaaggccg tcatctcccc cgccccctgc ccccggaagg
     1201 ccgcgtaggt gctcaaggcg acaatgggat tcacgatcgg cgccgccagc atgtaggtca
     1261 ccgcatggga aagcggcagg cccttggcga tcaaccggcg gatcaccggc acgacggcgc
     1321 attcgcacat gggaaaaatc attcccagca atccgctcac gcatgcgccc aggaagggat
     1381 tccgcggcag caggcgcgac atcaggcggg cgggcaaaaa ttggtcgatc aacccggaaa
     1441 tcgccgtgcc cagcaggatg aagggcacgc cctccagcag cacgctcagg aaggagtaac
     1501 aaaagtcggg aaaactgaaa ttaaaccatc cgggcatccg ggaaagaaac cacgaagtcc
     1561 gcggaattca cgtagaaaat ctttttgatt tcggatttgg gaatgcggat ttcggaatga
     1621 ttttcgaacg gagcgcgctg gcatttcatt ccgcaatccg cattccgaaa tccgaaatcg
     1681 ttaaatattc gtcccgagaa acgtgtggat gccgcattcg gttttgtcga agccggtcca
     1741 gcggccggcg cgctcgttgt cgccttcgct cgccggccgc gtgcagggct ggcagccgat
     1801 gctgcgatat ccccgctccg ccagcgcatt cgtggggatc gagttggcct tgagaaaatc
     1861 ctgaacccgc tcgcgcgtcc agttcgccat cgggttcatc ttgagaatgt aatgatcccg
     1921 cagcacgtcg aacttgtaga gttccagaat ccgggtcttc tcccgcccct cgctctgctg
     1981 gcggcgcagg ccggtgatcc acacatccag ctcgctcagc tttttctgga gcggaatcac
     2041 cttccgcagc gtgcagcaaa aatccggccg gcgcttccac agctccggtc cgtgctccgc
     2101 cgcctgctcc tcgacggttt gctcgggacg cagggattcg atctccaact gatagcgctc
     2161 ctccagcttt cgtttcaaat cgagggtctc gggaaagagc aatcccgtat ccagggtaaa
     2221 aatggggaag ttccagccgt tcgtctgggc cgtgtgaatg atgaccagtc ccgcgccctg
     2281 gaaactcgta ccaatggccg cccgggcgcc aaaacgcttc cagccccatt ccaaaattcg
     2341 ctccagcgga gcttcctcaa actcctggga cagggaatgg atctcttccg ccgaaatatg
     2401 gtcgagcgat aacgacatcc cgtgagaata cccccattcc caccgatttt ctagagatta
     2461 tcgtaatctc gaaaaaagcc acggacggct catcgagccg tccctgcctt ttaatgggca
     2521 gggcgggatc gccgagcccg ccgacttcgc ccccagctt
//
LOCUS       DATDAY010000262         4055 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_2464435, whole genome shotgun sequence.
ACCESSION   DATDAY010000262 DATDAY010000000
VERSION     DATDAY010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 4055)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4055)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4055
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2464435"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1109)
                     /locus_tag="VIS74_05205"
     CDS             complement(<1..1109)
                     /locus_tag="VIS74_05205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792674.1"
                     /translation="MKRSIWIFLLAIVLPSIGLGWLALRSANEQQFVLERRTAELYQK
                     ETDAAATAARSLIDEERRAFAERVRQLLASSSAPELAQDFAAKLSRAWPRPAIGFALS
                     ADGNLISPSAQEASADAGRQKFLLDNAAFLSGNTPATVYSVPAEELNRPQQRLKTSKS
                     ESEDKTAADSLAAKSVAKHKQPEAAPAGAAPAPAPAVKEKDDGRQEQQMRNVAPQRVL
                     KENLPAVSQLVPATADFKALTSDHPEGVVTRFMQDRLEIVFWLKPPAAAGMIFGCLIS
                     VDALTDLWRDALPENASPDYVLALLNDKGRPAVTQPPGETGRDWRRPFVASEIGEALP
                     HWEAALYLMRPQQLQENARGVRRTLLLLIAAALAA"
     gene            complement(1634..2962)
                     /locus_tag="VIS74_05210"
     CDS             complement(1634..2962)
                     /locus_tag="VIS74_05210"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEY5792675.1"
                     /translation="MKKLQLLGLAAALAQAASASAASDLTLRVTPERDYVYRLGPREV
                     IVQVEVEARKSDDVRRSPMNLSIVLDRSGSMEGAKLEKARQAAAMAVDKLADDDIFSL
                     VTYDDQTDLLIAPERVGNRDHREELKARIHRIQAGGSTALHAGVVLGSKQVRRFFEKE
                     RVNRVILLSDGIANVGPSSTSALSGLGRELRGEGIAVSTVGLGDDYNEDLMTALAEAS
                     NANYYYVKDAEKLPGIFAQELGAARSLLARNIVIRIQAPDGVRLKEIVGRPDITCGDR
                     VTEIKMPELFGSEKRRFLVRCLAEGKTADAIEAATVELNYATLSGGAAPAQRQAAKIS
                     FTDDQKKADASIQAEVARENSVVQNRLAKEMAVKLADEGKTKDAVALLRSQAAKNAAA
                     PAPMQVPGMKAENQKLEEAASEIDAGGRLDKSRRKAMQFENYADKYQKSR"
     gene            3279..4028
                     /locus_tag="VIS74_05215"
     CDS             3279..4028
                     /locus_tag="VIS74_05215"
                     /inference="COORDINATES: protein motif:HMM:TIGR00697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine precursor transporter"
                     /protein_id="HEY5792676.1"
                     /translation="MIRRLTARDARLSSFRFYDLLVHVFVVVLLISNLVGQKICAIPL
                     FNFQGELITAKISGAQLLFPITYIFGDIFTEVYGYGASRRAIWLGFFACAVLSLFGLV
                     IVAIPAAPDWPNQAAFEAVFYTVPRFILASLVAFWLGEFTNSYTLAKMKLWSEGKWLW
                     TRTVGSTVTGQLVDTTVLILIAFGGRESWMTMLNLIISGYIGKVLYEALATPVTYLVV
                     NKLKRSEGVDVFDRGTNFNPFARNTETAAGR"
BASE COUNT          740 a         1222 c         1226 g          867 t
ORIGIN      
        1 atcgccgcca gggcggccgc gatcaaaagc aggagggttc gccgtacgcc ccgcgcgttt
       61 tcctgcaatt gctgcggacg catgagataa agcgccgctt cccagtgcgg caacgcttcc
      121 ccgatttccg aagcgacaaa cggtcgcctc caatcgcgcc ccgtttcgcc cggtggctgg
      181 gtcacggccg gccggccctt gtcgttcaac agggcaagca cgtagtccgg cgaggcgttt
      241 tccggaagcg catcgcgcca gagatcggtc agcgcatcaa cgctgattaa acagccgaaa
      301 atcatgcccg ccgcggccgg cggcttcagc caaaagacga tctcaaggcg atcctgcatg
      361 aaacgcgtga cgacgccttc cgggtgatcg gacgtaaggg ccttgaagtc cgccgtcgcg
      421 ggcacgagct gcgaaaccgc gggcaaattt tccttgagga cacgctgcgg ggcgacgttc
      481 cgcatttgct gttcctgtct gccatcatct ttctctttaa cggccggtgc gggggctggc
      541 gccgctccag cgggagcggc ttcgggttgc ttgtgtttgg ccaccgactt cgccgccaac
      601 gaatccgccg ctgttttgtc ttcggattcg ctctttgagg tcttgagccg ttgttgtgga
      661 cggttcaact cctccgccgg aacggagtaa acagtcgcgg gcgtgttccc ggagagaaac
      721 gcggcgttat cgaggagaaa tttctgccgt ccggcatcgg cgctcgcttc ctgggcggaa
      781 ggcgaaatga gattaccgtc cgcgctcaag gcgaacccga tcgccgggcg cggccaggcg
      841 cgcgagagct tggcggcgaa atcctgcgcg agttcgggcg ctgatgaact cgcgagcaat
      901 tgacggactc gctcggcaaa cgcgcgccgt tcctcgtcga tcaagcttcg cgccgcagtc
      961 gcagcggcgt ccgtttcctt ttggtaaagc tccgcggtgc ggcgctcgag cacgaactgc
     1021 tgttcgttcg cgctccgcag ggccagccaa cccagaccga tgctcggcag gacgatggcg
     1081 aggagaaata tccagatgct tcgtttcacg aggcgaggcg cagggtatca cgttcccgtt
     1141 cgtctgtgct cccgaaaata gccccgcgtt ttaacggtgg gaaaatcatg tgagccaaat
     1201 gctgaagtcc cttcagggac gaaagaagcg ccgacgaaca acgaaggtct gccgtccctg
     1261 aaggaacttg tatgattgtt ttgatcgaaa cccagcgttg aaacgctggg ctattttcag
     1321 agagcgtacg ccgaatgcac gcaacgcgcg gagggctttg ttgaggaagg catttgcttt
     1381 catcgaacgt tacgtagcga actggtaggg acggcgcgcc cttcgattgg aactcagggc
     1441 cttgagctta atcgaaacgg ctgagggtcc catgcagggg ttggttaaga cgattcaagt
     1501 tttgtttcct cgtgctcccc tgcgtggcgg acgccgcccc tacccgggca gtctgcagac
     1561 cgcggcctgc aggctgcgcg cgagggaaga gcctgaaacc ctcacgcggc ccacagcgcc
     1621 gcggctacag aaatcagcga ctcttctggt acttgtccgc gtaattctcg aactgcattg
     1681 ctttccggcg cgacttatcg agccggccac ctgcatcaat ctccgacgca gcctcctcga
     1741 gcttttgatt ctccgccttc attcctggaa cctgcatcgg ggcgggggca gccgcgttct
     1801 tggcggcttg cgaacgaaga agcgccaccg cgtccttcgt cttgccttca tccgccagtt
     1861 tcaccgccat ctctttggcg agccggttct gcacgacgct gttttcccgc gcgacctccg
     1921 cctggatgct cgcgtccgct tttttctggt cgtcggtaaa ggaaatcttt gccgcctgcc
     1981 gttgcgcggg cgccgcgccc ccggacaggg tggcgtagtt caactccacc gtggctgctt
     2041 caatcgcatc cgcggttttt ccttccgcca ggcaacgcac gaggaaccgc cgtttttccg
     2101 aaccgaacag ctccggcatt ttgatttccg tcacccggtc gccacaggtg atgtcgggcc
     2161 gtccgacgat ttccttcagg cgcacgccat ccggcgcctg gatgcgaatg acgatgttac
     2221 gggcgaggag cgagcgcgcc gcgcccaatt cctgcgcgaa aatgccgggc aatttctcgg
     2281 cgtccttcac atagtaatag ttggcgttgc tcgcctcggc cagggcggtc atgaggtctt
     2341 cgttgtaatc atcccccagg ccgacggtgc tgactgcgat gccttcgccg cgtaattcgc
     2401 ggccgaggcc ggagagggcc gaggtgctgc tcggtccaac gttggcgatg ccgtccgaga
     2461 gcaggatgac gcgattgacc cgctcctttt cgaagaagcg gcggacctgc ttcgagccca
     2521 agaccacgcc ggcgtgcagc gcggtgctgc cgcccgcctg gatgcgatgg atacgcgcct
     2581 tgagttcttc gcgatgatcc ctgttgccaa cgcgctcagg cgcgatgagc aaatcggttt
     2641 ggtcatcata ggtgacgagc gaaaagatat cgtcgtcggc cagcttatcg acggccatgg
     2701 cggcggcctg ccgcgctttc tcgagcttcg ccccttccat ggaaccgctg cggtccagga
     2761 cgatcgagag attcatcggc gagcgccgca cgtcgtcgct tttgcgggcc tcaacctcga
     2821 cctgcacgat aacttcgcgc gggccgagcc ggtaaacgta atcgcgttcc ggcgtgaccc
     2881 gcagggtcag atccgacgcg gctgaagcgg aggctgcttg cgcgagcgct gccgcgagac
     2941 cgagtaactg tagtttcttc ataactccat tagacaggag ttcgcgaaac actgtgtcag
     3001 agccgcgtgc gaagtttgta aaagaaatgt cagagtgtca ggggactttc taaccgcgga
     3061 ttacacggat gactcggatg gggattcaaa cacttagagc gctgtagccg cgtcgctatg
     3121 tcgacgcgtt gcgcgttggt tgcctggaaa gcgtccggaa cggcgatccc ggcgcggcgg
     3181 gaccgtcgct acagcgctga atgttcggcg cggctgacca caaaaacatc cgtgtcatcc
     3241 gcgtaatccg cggttaacaa ttcctgatgg ccgagacgat gatccgccga ttgaccgcgc
     3301 gcgatgcgcg gctgagcagc tttcgattct acgatctgct cgttcacgtc tttgtcgtcg
     3361 tcctcctgat ctcgaatctg gttgggcaaa agatctgcgc gatcccgctc ttcaattttc
     3421 agggtgagct catcacggcg aaaatctccg gggcgcaatt gcttttcccg atcacctaca
     3481 tctttggcga catcttcacg gaagtttacg gttacggcgc gtcgcgccgg gcgatctggc
     3541 tgggattttt tgcgtgcgcg gttctttcgc tttttggttt ggtcatcgtg gcgattccgg
     3601 cggcgccgga ttggccaaac caggcggcgt tcgaggcggt cttttatacc gtgccccgct
     3661 ttatcctcgc cagtctcgtc gcgttctggc tcggcgaatt caccaactct tacacgctcg
     3721 cgaaaatgaa gctttggagc gaaggcaaat ggctctggac ccggaccgtc ggctcgaccg
     3781 tgaccggcca actggtcgat acgaccgtcc tgatcctgat cgcgttcggc ggacgtgaat
     3841 cctggatgac gatgctgaat ctgatcattt ccggttacat cggcaaagtt ctctatgaag
     3901 cgctggcaac accggtgacc tatctcgtcg tgaacaaatt aaaacggtcg gaaggcgtgg
     3961 atgttttcga tcgaggcaca aatttcaatc cgttcgcgcg caatactgaa actgctgctg
     4021 gacgataact accgaggact cagagagcgc agagg
//
LOCUS       DATDAY010000263         3751 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_2284126, whole genome shotgun sequence.
ACCESSION   DATDAY010000263 DATDAY010000000
VERSION     DATDAY010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 3751)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3751)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3751
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2284126"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1127
                     /locus_tag="VIS74_05220"
     CDS             <1..1127
                     /locus_tag="VIS74_05220"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEY5792677.1"
                     /translation="IAASSLTLLLIWRGYFQAGFLLQTFASIVVFTITGTIYFNASEN
                     YLLLTMAVVVFVMEPKSRRIPVAMVCALAFVLVKISHFASFETGRVPLDRYVLNIFIF
                     TLAFYAFLEIFRKLIGDYLARIEASNAELARQRIELHEERARLKRANQAKEKLFSMVA
                     HDLRGPVGNLKTAMEGLEEGFLSGEDFRGLQADLRLGVKRVYASLDDLLLWAAGQMHG
                     IEAELENVPLKAVAQEMIELLQATADAKRITLRNGLAEEVVVRADRHQIGTVIRNLLA
                     NAIKFTPESGAVEVSAAREGARWKVAISDNGVGMNAEVLARLFNSEVAESAPGTRNEK
                     GWGLGLQICRDFITANGGRIWAESRPRRGTTFHFTLASAE"
     gene            complement(1152..1280)
                     /locus_tag="VIS74_05225"
     CDS             complement(1152..1280)
                     /locus_tag="VIS74_05225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792678.1"
                     /translation="MKNLKSILTVAAVAAFALVSLQGCASKKSCCMTKPTTASYSK"
     gene            complement(1286..2302)
                     /gene="cydB"
                     /locus_tag="VIS74_05230"
     CDS             complement(1286..2302)
                     /gene="cydB"
                     /locus_tag="VIS74_05230"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HEY5792679.1"
                     /translation="MDINTTWFILVGVLLTGYAILDGFDLGVGVLHLFVKKDEHRRLM
                     LNSIGPVWDGNEVWLVTGGGALFAAFPEAYATVFSGFYIPFMLLLAALIFRAVAIEFR
                     SKEPWRWWRRMWDIAFAGGSFLSALLIGVAMGNIVRGVPLDAEFEFRGTFLSLLNPYS
                     LLLGVTTVLLFAMHGAIYVAMKTEGELQATIRRWIPGLIGTFLAAYFLFNLYTLVECQ
                     HVLEAVRARPYILPVMAVGAFIILNIPREIHHGREFRAFLSSCLGMAIMMGAFGLTYF
                     PNMVFSNPNPANSLTIYNAASSEKTLRIMLLIAVIGVPIVLAYTASIYYIFRGKTKLS
                     SHSY"
     gene            complement(2306..>3751)
                     /locus_tag="VIS74_05235"
     CDS             complement(2306..>3751)
                     /locus_tag="VIS74_05235"
                     /inference="COORDINATES: protein motif:HMM:NF013791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HEY5792680.1"
                     /translation="IGMGVVLVYLEGMYLRTREAIYEEAARFWTKIFAVNFAIGVATG
                     IVMEFEFGTNWSVYSRFVGDVFGSALAAEGIFAFFLESGFLAILLFGWNKVGPKLHFF
                     ATCMVCLGAHFSALWIVVANSWMQTPAGYHLEKMVNGQPVRLPPDYVVAAGDLGTVRA
                     VIDDFWTMVFNPSALDRITHVLLGAWMAGAFLVLSISAYYLLRGRHLEFAKASLKVGL
                     VFAAAATVLQMVSGDFTAKGLARNQPIKLAALEGLYETTAYAPLNLIGWADSTHERDF
                     GLKIPGLLSFLTHGDFATPVAGLRDLPPDEFLQKRHPGATPAELTKIRRSYWPNVPAV
                     FQFYHLMISVGVALFALVVIGCFRWWRGWLFDTRSKGSRLFLMALVLSVLGPQIANQA
                     GWFAAEMGRQPWIVYELLKTSQAFSRVVSAEHIVFSLLLFAFVYALLFAVFIYILTRK
                     IHHGPDEEEASHEMPESWRNLIRRGAARAEA"
BASE COUNT          816 a         1072 c         1213 g          650 t
ORIGIN      
        1 tgatcgccgc cagcagcctg acgctgctgc tgatctggcg cggttatttc caagcggggt
       61 ttttattgca gacgttcgcg tcgatcgtgg tattcaccat taccgggacg atttatttca
      121 acgcgtcgga gaattacctg ctgctgacca tggcggtcgt ggtgtttgtc atggagccaa
      181 aatcccggcg cattccggtg gcgatggttt gcgctctcgc gttcgtgctg gtcaaaattt
      241 cccatttcgc gagtttcgag accgggcggg tgccgctgga tcgttatgtg ctgaacatct
      301 tcatcttcac gctggcgttt tacgccttcc tggaaatctt tcgcaaattg atcggggatt
      361 acctcgcgcg gattgaggcc agcaatgccg agctcgcccg ccagcggatc gaactccacg
      421 aagagcgcgc ccggctgaag cgggccaacc aggcgaagga aaagttgttt tccatggtcg
      481 cgcatgatct gcgggggccg gtgggaaatc tcaaaacggc catggaaggg ctggaggaag
      541 gattcttgag cggggaggat tttcgcggcc tgcaggccga tctccggctg ggcgtgaagc
      601 gggtttatgc ctcgcttgat gatctattgc tctgggcggc gggacagatg cacggcatcg
      661 aggcggagtt ggaaaatgtc cccttgaagg ccgtcgcgca ggagatgatc gagctgctgc
      721 aggcaaccgc ggacgccaaa cgcatcaccc tgcgaaatgg cctggcggaa gaagtggtcg
      781 tgcgcgccga ccggcatcag atcggcacgg tcattcgcaa cttgctggcg aacgccatta
      841 aattcacccc ggagagcggc gcggtcgaag tgtcggcggc ccgggaaggc gcccgctgga
      901 aggtggccat ctccgacaat ggcgtgggca tgaacgccga ggtgctggcg agactcttta
      961 attccgaagt ggcggagtca gcgccgggaa cccgcaatga aaagggctgg gggctgggat
     1021 tgcagatttg ccgggacttc atcacggcga atggcggccg aatctgggcg gaaagcaggc
     1081 cgaggcgggg gacgaccttc catttcacgc tggcatccgc cgaataaacg gcggatgccg
     1141 cgtttaaccg cttacttcga gtagctcgcc gtcgtcggct tggtcatgca gcaggatttc
     1201 ttgctggcgc aaccctggag gctgacgagg gcgaaggcgg caaccgccgc cacggtgagg
     1261 atggatttaa ggttcttcat aggtattagt agctgtggga ggacagtttc gtcttcccgc
     1321 gaaaaatgta ataaatgctg gcggtgtaag ccagcacgat cggcacgcca atgacggcga
     1381 tgaggagcat gatgcgcagc gtcttttcgc tggaagcggc attgtaaatc gtcaggctgt
     1441 ttgccggatt cggattggaa aacaccatgt tcggaaaata cgtcaggccg aaggcgccca
     1501 tcatgatcgc catgcccagg caggaggaga ggaacgcccg gaactcgcgg ccgtggtgaa
     1561 tctcgcgtgg aatattgaga atgatgaacg cgcccacggc catgacgggg aggatgtaag
     1621 gccgcgcgcg caccgcctcg agcacgtgct ggcattccac cagcgtgtag agattaaaca
     1681 ggaaataggc ggccaggaag gtgccgatga ggccgggaat ccagcggcgg atggttgcct
     1741 gaagttcgcc ctccgttttc atggccacgt agatcgcgcc gtgcatggcg aaaagcagca
     1801 ccgtggtcac gcccagcagc agcgaatagg gattcagcag gctcagaaac gtgccgcgga
     1861 attcaaactc cgcatccagc ggcactccgc gaacgatatt gcccatggcg acgccgatga
     1921 gcagggcgga aagaaaactg ccgccggcga acgcgatgtc ccacatccgc cgccaccagc
     1981 gccacggctc cttgctgcga aattcgatcg ccacggcgcg aaagatcagc gcggccagca
     2041 gcagcatgaa gggaatgtaa aagccggaaa agacggtggc gtaggcttcg ggaaacgcgg
     2101 cgaagagcgc gccgccgccg gtcacgagcc agacctcgtt gccgtcccag accgggccga
     2161 tggaattgag catcagccgc cggtgctcgt cctttttgac gaataaatgg aggacgccca
     2221 cgccgaggtc gaagccgtcg agaatggcgt aaccggtcag cagaacgccc acgagaatga
     2281 accaggtcgt attgatgtcc atcggtcagg cctccgcgcg agccgcgccg cggcggatca
     2341 ggttgcgcca gctttccggc atttcgtggc tggcctcctc ctcgtccggg ccgtggtgaa
     2401 tcttccgggt gaggatgtaa ataaacacgg cgaagagcag cgcgtaaacg aaggcgaaga
     2461 gcagcaggga aaagacgatg tgctccgcgc tgaccacccg cgaaaacgcc tgcgaggtct
     2521 tgagaagctc atacacgatc cacggctggc ggcccatttc cgcggcgaac cagccggcct
     2581 gattcgcaat ctgcgggccg agcaccgaga gcaccagcgc cattaaaaag aggcgcgagc
     2641 ctttcgaccg cgtgtcgaaa agccagccgc gccaccagcg gaagcagccg atgacgacga
     2701 gggcaaacaa cgccacgccg acggaaatca tgagatgata gaattgaaac accgccggca
     2761 cattgggcca gtaactccgg cggattttcg ttaattccgc gggcgtcgcg ccgggatggc
     2821 gtttttggag aaattcatcc ggcggcaaat cccgcagccc ggcgaccggc gtggcgaaat
     2881 cgccgtgcgt gagaaaactg agcagtccgg ggattttgag gccgaaatcc cgctcgtggg
     2941 tggaatccgc ccagccgatg aggttgagcg gcgcgtaggc ggtggtttcg taaagcccct
     3001 ccagcgcggc gagcttgatg ggttgatttc gggcgaggcc cttcgcggtg aagtcaccgc
     3061 tgaccatttg gagcaccgtg gcggcggcgg caaacacgag gccgaccttc agcgaggcct
     3121 tggcgaattc gagatggcgg ccgcgcagca ggtaatacgc gctgatgctc agcaccagaa
     3181 aggctccagc catccacgcc cccagcagca cgtgggtgat gcgatccaaa gccgaagggt
     3241 tgaacaccat cgtccagaaa tcatcaatga cggcgcgcac cgtgccgaga tcgccggcgg
     3301 ccacgacgta atccggcggc agccgcaccg gctggccgtt caccattttt tccagatggt
     3361 aaccggcggg cgtctgcatc cacgaattcg cgacgacgat ccacagcgcg ctgaagtgcg
     3421 cgccgaggca gaccatgcac gtcgcgaaga aatgcagttt cgggccgacc ttgttccagc
     3481 cgaagagcag gatggcgagg aagccggatt cgagaaaaaa ggcgaagatg ccctcggccg
     3541 cgagcgcgct gccgaaaacg tcgccgacga agcgcgaata aaccgaccag ttcgtgccga
     3601 actcgaattc catgacgatg ccggtcgcca cgccgatggc aaaattcacc gcaaagatct
     3661 tggtccagaa ccgggccgct tcctcgtaaa tcgcctcgcg ggtccgcagg tacatccctt
     3721 cgagatacac cagcacgacg cccatgccga t
//
LOCUS       DATDAY010000264         2267 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_2323145, whole genome shotgun sequence.
ACCESSION   DATDAY010000264 DATDAY010000000
VERSION     DATDAY010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2267)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2267)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2267
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2323145"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(108..800)
                     /locus_tag="VIS74_05240"
     CDS             complement(108..800)
                     /locus_tag="VIS74_05240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792681.1"
                     /translation="MKNKILAALLIWTVLAGFAVLLVWARQPLPPAAARPDRAAQGGA
                     APANHAPEPERPGGAAGIRPAAPPSISSAAPPPPVATSAGPKPKVDEKPAEQTAAGIY
                     FLPEIAEISRNLNAPRGSVEEDVMIIRGVIEIYRRNNAGAVPEGFDNAQITAKLRGEN
                     ARRFSVLDPNLESSALNAKGELLDRWGTPYYFHKASRELLEVRSAGPDGKFWSRDDVP
                     AGPFAPGPFDSN"
     gene            complement(797..>2267)
                     /locus_tag="VIS74_05245"
     CDS             complement(797..>2267)
                     /locus_tag="VIS74_05245"
                     /inference="COORDINATES: protein motif:HMM:NF019040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEY5792682.1"
                     /translation="FSRRSFIRQSACAALGYAGVVNALTQLSIMNSALGQSSLDGYKA
                     LVVLFLGGGNDSNNMLIPRMGHPEYANYLASRRVLSIFDPADPARGSAPACIPLTTAD
                     GNYGLHPNLQHLANIFHAGDLSFVTNVGTLAFPIPTRDDYINERIPIPPQLGSHADQV
                     IQWQSSLPDKPFLTGWGGRVADLLNANTPDSISMSISVAGINKFQVGLETAQYNIDAS
                     GSIPGLTGYGGGYSWAMSNNTYNTSVHGRRLRAFDDILRYTRSNLIEEGYSKVVRKSR
                     DTEALLGSAFTAAASSGVNFDTLFGNATTGLSAELKTISKLIAGRNALGNNRQIFFAA
                     VGGYDTHGGQMAAHSNLMNELSTSLKAFRDAMIALGMNDNVLTITNSDFARTLQPNGT
                     DPGTGSDHGWGGHHIVMGGGVNGSRIFGQFPTLATGSSAGSIDSRDRGTWIPTTSVDQ
                     YAAVAAKWLGVESSHLSLAFPNLGRFDDPFGSTANLLYV"
BASE COUNT          399 a          642 c          807 g          419 t
ORIGIN      
        1 ctcgcgtcaa atgaagcaac ggacggctcg gcgagccgtc cctgcccatt aaaaggtagg
       61 gcgggttcga tgaacccgcc gtggctcgct ttgcaaaata aacgccgtca gttcgaatca
      121 aacggtccgg gcgcgaacgg gccggcgggg acgtcgtctc tgctccaaaa tttgccatcc
      181 ggcccggcgg aacgcacttc gaggagctcc cgggaagcct tgtgaaaata ataaggggtg
      241 ccccagcgat ccagcagctc gcccttcgcg ttgagggccg aggattcgag attgggatcc
      301 aggacggaaa aacgccgggc gttctcgccg cgaagtttgg cggtgatttg cgcattatcg
      361 aagccttcgg gaacggcgcc tgcgttgttc cggcggtaga tttcaatcac tccgcggata
      421 atcatcacgt cttcctcaac gctgcctcgc ggggcgttga ggttgcggct tatctcggcg
      481 atctcgggaa gaaaatagat gcccgccgcc gtttgttcag ccggcttttc gtccactttt
      541 ggcttcggtc ccgcggaagt tgcgaccggc gggggggggg ccgcactgct tatggaaggc
      601 ggggccgccg gccggattcc ggcggcgcca cccgggcgtt ccggctcggg ggcgtgattg
      661 gccggcgccg cgccgccttg ggccgctcga tccggccgcg cggccgcggg cggcaggggt
      721 tgccgcgccc agactaaaag gacggcgaac ccggcgagga ccgtccagat caggagggcg
      781 gcgaggatct tgttcttcaa acgtagagca gattggccgt ggagccgaag ggatcgtcaa
      841 agcgtccgag gttgggaaag gccagcgaca ggtggctgga ttccaccccc agccatttgg
      901 ccgccacggc cgcatattga tcgacggaag tggtggggat ccaggtgccg cggtcgcgcg
      961 agtcgatcga accggccgac gatccggtcg ccagcgtggg gaactgccca aagatgcgcg
     1021 atccattcac tccgccgccc atgacgatgt ggtggccgcc ccagccgtgg tcggagccgg
     1081 tgccgggatc ggtgccgttg ggctgcaggg tgcgggcgaa atccgaattg gtgatggtca
     1141 ggacgttgtc gttcattccc agcgcgatca tcgcgtcgcg gaaggctttc aacgacgtgg
     1201 agagttcgtt catgaggttc gaatgggccg ccatctggcc gccgtgcgtg tcgtagccgc
     1261 ccacggcggc aaagaagatc tggcggttgt tgcccagcgc gttgcggccg gcgatcaact
     1321 tggagatcgt cttcaactcc gccgacagcc cggtggtggc gttgccgaag agagtgtcga
     1381 aattcacgcc gctcgacgcc gcggcggtga acgcggagcc caacaatgcc tcggtgtcgc
     1441 gggattttcg gacgactttg ctatagcctt cctcgatgag gttggaccgg gtgtagcgca
     1501 ggatgtcgtc aaatgcgcgg agccgccgcc catggacgga ggtgttgtaa gtgttgttgc
     1561 tcatggccca gctatagccg ccgccgtatc cggtcaggcc gggaatgctt ccgctggcgt
     1621 cgatgttgta ttgcgcggtt tccagcccga cctggaattt gttgatcccc gccaccgaga
     1681 tggacatcga gatcgaatcg ggggtgttcg cgttcaacag gtccgcgacg cgcccgcccc
     1741 agcccgtcag gaagggtttg tccggaaggg atgactgcca ttggataact tggtccgcgt
     1801 gggagcccag ttgcggagga atggggatgc gttcgttgat gtagtcgtcc cgggtgggaa
     1861 tgggaaacgc cagggtgccg acgttcgtca cgaaggagag gtcgcccgcg tgaaaaatat
     1921 tggccaggtg ctgcaggttg ggatgcaggc catagttgcc gtcggcggtg gtcaggggaa
     1981 tgcacgccgg cgcgctgccg cgggcgggat cggccgggtc gaagatcgac agcacgcggc
     2041 gcgaggcgag gtaattggca tactccggat ggcccatccg ggggatgagc atgttgttgg
     2101 agtcgttgcc gccgcccaaa aatagaacga ccagcgcctt gtagccgtcc agggaggatt
     2161 gcccgagcgc ggagttcatg atgctgagct gggtgagcgc gttgaccacg ccggcataac
     2221 cgagcgcggc gcaggcggat tgccggatga aactccggcg ggagaat
//
LOCUS       DATDAY010000265         2003 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_1263785, whole genome shotgun sequence.
ACCESSION   DATDAY010000265 DATDAY010000000
VERSION     DATDAY010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2003)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1263785"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..384
                     /locus_tag="VIS74_05250"
     CDS             <1..384
                     /locus_tag="VIS74_05250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792683.1"
                     /translation="EDEPALDIPVMQGEDVRGLRIPQYGPDGKLQMQFSAEIARKLDN
                     RTLELEKLNVEVVDKEGTVTVEIPKSRFDLETRVLTGNDGAKIKRQDFEITGETVEFY
                     VRTRFSRLGGNVKMVIHSFDSVKNE"
     gene            377..949
                     /locus_tag="VIS74_05255"
     CDS             377..949
                     /locus_tag="VIS74_05255"
                     /inference="COORDINATES: protein motif:HMM:NF015901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptA/OstA family protein"
                     /protein_id="HEY5792684.1"
                     /translation="MSKHTWICLAAALTLAPALAQETPTLNVKSEGKRDALGMSALTK
                     DRPKDAQTEITARNHATFDNETNVAEFEGSVVVRDPQFTLTCDKLRVFLRPDRKGMQK
                     VEAIGNVLIKQENVTERGEKATSTGQAGKAIYTPEDGNVVLTEWPQIERGINVHIATE
                     KGTVMTLNRDGRMNTQGGSKTLIKDTSQTP"
     gene            949..1725
                     /gene="lptB"
                     /locus_tag="VIS74_05260"
     CDS             949..1725
                     /gene="lptB"
                     /locus_tag="VIS74_05260"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HEY5792685.1"
                     /translation="MPTSAPSPSPVAPAANLHEDILQTRQLVKIYGGRAVVNGVDIHV
                     KRGEIVGLLGPNGAGKTTTFYMIVGLVRPQSGTVLFENRDVTQMPMFQRARQGMGYLP
                     QEESIFRKLTVEQNIMAILETTKMGKAERKARCEQLLNQFGIEHLAKNYALTLSGGEK
                     RRLTIARSLVTNPSLLMLDEPFSGVDPIAVYDVQQIIQDLRKEGLAILITDHNVRETL
                     NIVDRAYLIFEGRVESQGDKDFLLNDPISRKLYLGETFTM"
     gene            1722..>2003
                     /locus_tag="VIS74_05265"
     CDS             1722..>2003
                     /locus_tag="VIS74_05265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HEY5792686.1"
                     /translation="MTRSNTKRIASISVGHFYANHAAHLSLRLEGASKGMSRPIREPT
                     INRPGLALMGFYSYFAAKRIQVIGAAELSYLRSLEPEILATRLQSLFARS"
BASE COUNT          466 a          630 c          579 g          328 t
ORIGIN      
        1 gaggacgagc cggccctgga catccccgtc atgcagggcg aggacgtgcg cggcctgcgg
       61 atcccgcaat atgggccgga cggcaaactc caaatgcaat tctcggcgga aatcgcccgc
      121 aagctcgaca accggacgct cgaactcgaa aaattgaacg tcgaggtggt ggacaaggag
      181 ggcacggtga cggtcgagat tcccaagtcc cgttttgatt tggaaacccg ggttctgacc
      241 ggaaacgacg gggcaaaaat caaaagacag gactttgaaa ttaccggcga aacggtagag
      301 ttttacgttc gcacccgttt cagccgcctc ggcgggaacg tcaaaatggt cattcacagc
      361 ttcgattccg tcaaaaatga gtaaacacac ctggatttgt ctggccgccg cgctgaccct
      421 ggcacccgcc ctggcgcagg aaacccccac cctgaacgtg aaaagcgagg gaaagcggga
      481 tgccttgggc atgagcgcgc tcaccaagga ccgccccaag gacgcgcaga cggaaatcac
      541 cgcccggaat cacgccactt tcgataacga aaccaacgtc gcggagttcg agggctcggt
      601 cgtcgtgcgc gacccccaat tcaccctgac gtgcgacaaa ctgcgggttt ttctccggcc
      661 ggaccgcaag ggcatgcaaa aggtggaagc catcggcaat gtcctcatca agcaggaaaa
      721 cgtgaccgag cgcggggaaa aagccacttc caccggccag gcgggcaagg cgatttacac
      781 gcccgaggac ggaaacgtcg tgctcacgga gtggccgcag atcgaacgcg gcatcaatgt
      841 ccacatcgcc accgaaaaag ggacggtcat gacgcttaat cgcgatggga gaatgaacac
      901 gcagggtggc agcaaaaccc tcatcaaaga caccagccag actccataat gcccacttcc
      961 gccccttcac cttctcccgt ggctcccgcc gcgaatttgc acgaagacat cctgcagacc
     1021 cggcagctgg tcaaaatcta cggcggccgg gccgtggtca acggcgtgga catccacgtc
     1081 aagcgcggcg agattgtcgg cctcctcggc cccaacggcg cgggcaagac aaccactttt
     1141 tacatgatcg tcggactcgt ccggccgcag agcgggacgg tgcttttcga gaaccgggat
     1201 gtcacgcaga tgccgatgtt ccaacgcgcc cggcagggaa tgggttacct gccgcaggag
     1261 gaatccatct tccgcaagct taccgtcgag caaaacatca tggcgatcct cgaaacgacc
     1321 aagatgggca aggccgaacg caaggcgcgc tgcgagcaat tgctcaacca gttcggcatc
     1381 gagcacctcg cgaaaaatta cgcgctcacg ctgagcggcg gcgagaagcg ccgtctgacc
     1441 atcgcccgtt ccctcgtcac caatccgtcg ctcctgatgc tcgacgaacc cttcagcggc
     1501 gtggatccga tcgccgttta cgacgtgcag cagatcatcc aggatctgcg caaggagggc
     1561 ctggccattc tcatcacgga tcacaatgtc cgcgagacgc tcaacatcgt ggaccgcgcg
     1621 tatctcattt tcgaaggccg ggtggagagc cagggagaca aggattttct gttgaacgat
     1681 cccatcagcc gcaaattgta tctcggcgaa acgttcacga tgtgacccgg tcgaacacca
     1741 agcggatcgc ctccatctcg gtcggccatt tttacgccaa ccacgccgcg catctcagcc
     1801 tgcggctgga gggcgcgtcg aaggggatga gccgccccat ccgcgaaccc accatcaacc
     1861 gccccggcct ggcgctgatg gggttctaca gttactttgc cgccaagcgg atacaggtca
     1921 tcggcgcggc ggagctcagt tatctgcgca gcctggagcc ggaaatcctc gccacgcggc
     1981 tgcaaagcct gtttgcgcgc agc
//
LOCUS       DATDAY010000266         4986 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_173983, whole genome shotgun sequence.
ACCESSION   DATDAY010000266 DATDAY010000000
VERSION     DATDAY010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 4986)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4986)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4986
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_173983"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..774)
                     /locus_tag="VIS74_05270"
     CDS             complement(<1..774)
                     /locus_tag="VIS74_05270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792687.1"
                     /translation="MSLLLTQFGRSEEMAPMRLPVVKTWSELSALPPLIDEPVPAFDA
                     RGKDAEPPKDGGDERIRIRVGIDRISARLNEGVLLYCLMETPRLPNEAVTDDDSLGPL
                     KVRVEFPGAAPALSQMAINKTDVATRDGRYFFMKTIPLDQAGTYRIRVFPPSNLTEEK
                     PLAEVRVEVSGERAPSWSPWSASSELGVNESAWEETEEGEPVALVQVSNPPGGAALPA
                     IDGWVLQPLPEKIDPASPLPELLPEKPASGIRAQAQENRL"
     gene            1455..2366
                     /locus_tag="VIS74_05275"
     CDS             1455..2366
                     /locus_tag="VIS74_05275"
                     /inference="COORDINATES: protein motif:HMM:NF007214.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma-70 factor"
                     /protein_id="HEY5792688.1"
                     /translation="MTNQGVELFEKHRPFLFGIAYRMLGSAADAEDMVQETFLRWQKN
                     EPGELKSAEAWLATVVTRLCINHLKSARVQREKYVGAWLPEPIVADYATANPRENAEQ
                     AESLSIAFLVILETLSPTERAVYLLKEVFGYEFGEVARIVRKSEPNCRQLLSRARARI
                     AERRPRFQSSADQQRRLVEAFLKAINEGDVAGLLAILSEEVTLITDGGAETPAPRRTI
                     AGADSVSRMLLRGAPREAAAATVTRLVSINGWPGFLACRGQTPRAAIAFDIDGERIRT
                     IYLIANPEKLASLPGGRPKTDASFDRN"
     gene            2442..2615
                     /locus_tag="VIS74_05280"
     CDS             2442..2615
                     /locus_tag="VIS74_05280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792689.1"
                     /translation="MKLASTRLVTNDVPALAQFYSAITGLNPVGIEDYVELDGNLISF
                     FASSAKPTQPIKI"
     gene            2612..2968
                     /locus_tag="VIS74_05285"
     CDS             2612..2968
                     /locus_tag="VIS74_05285"
                     /inference="COORDINATES: protein motif:HMM:NF024953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEY5792690.1"
                     /translation="MKYKNTLSWIVPALISFFMLFSAYISGTRVVEFAQLGFPEYFRI
                     ELTTAKVIGALLLLIPPVPARIKEWIYVGFGVVLISAAIAKFSSGYPMIGVLEPLIVF
                     TIMAASLLYLNKLAKT"
     gene            2996..3610
                     /locus_tag="VIS74_05290"
     CDS             2996..3610
                     /locus_tag="VIS74_05290"
                     /inference="COORDINATES: protein motif:HMM:NF024852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY5792691.1"
                     /translation="MNLFILGATGAIGKHLLDIALERGHRVTAYVRSPRKIPLAHERL
                     KVIPGDVFNAGQLAHSLAGHDAVLSAFGPTTIRSSTLRREFGRTLAAAMRKGGVRRVQ
                     LVSAAFLFPDIGFLGGLLKATLFRRMIPDMAGMEAEVSQSDLAWTMVRPPRLTNGPIS
                     GAYRILDGALPPGGFYISRADVARFMIGEAENPVHARRIVGLAD"
     gene            complement(3679..3891)
                     /locus_tag="VIS74_05295"
     CDS             complement(3679..3891)
                     /locus_tag="VIS74_05295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792692.1"
                     /translation="MRRNKADEDAVCDRCGRPAALRFEGEWLCAECVQARGSCCLEFG
                     SDDLWRFDETDVPASNRAPAARGAKG"
     gene            complement(3990..>4986)
                     /locus_tag="VIS74_05300"
     CDS             complement(3990..>4986)
                     /locus_tag="VIS74_05300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792693.1"
                     /translation="LAVDPTAAGDHLKKRARHLARSGFVSMIRPASMERKPRIYLLDP
                     YDPDKIPLLMVHGLQSTPVAFAALVNALRADPAIRANYQIWQFYYASGTPVLANAAAL
                     REALGKTVKLLDPENRDAATRKVVVLGHSMGGVISHTLVSSSGNRVWRSVFRVPTGQL
                     SGEPETVRALERILYFERNPRVQRVLFMAAPHRGSSMADSWIGTLGNFLARLPQLEER
                     GFTELARANPGAMMPGAAEFYAAGRFSAVRTLSPKSTALLALSELPVEVPFHSVIGQR
                     RPGPKETGSDGVVPYGSSHLAGAESELVVRGGHGVIGNDEGIAEVIRILRRNGRR"
BASE COUNT          941 a         1645 c         1481 g          919 t
ORIGIN      
        1 caggcgattc tcctgggcct gggcgcggat tcccgaagcg ggcttttcgg ggaggagttc
       61 cggcaggggc gacgcgggat cgatcttctc cgggagcggc tgtagcaccc acccgtcgat
      121 cgccggcagg gccgcgccgc cgggaggatt ggagacctga acgagcgcca ccggttcgcc
      181 ctcctccgtt tcctcccaag ctgactcatt caccccaagt tccgatgagg cagaccacgg
      241 cgaccagctt ggcgcccgtt ccccggaaac ctccacccgg acttcggcca atggtttttc
      301 ttccgtcaaa ttggacggcg ggaaaacgcg gatacgataa gtcccggcct gatccaacgg
      361 aatcgtcttc atgaagaagt agcgcccatc cctcgtcgcg acatccgtct tgttgattgc
      421 catctgggaa agcgctggag cggctccggg aaactccacc ctcaccttca gaggccccag
      481 gctgtcgtca tccgtgaccg cctcattggg cagccgcgga gtttccatga ggcaatagag
      541 cagcaccccc tcgttcagcc gcgcggaaat ccggtcaatg cccacgcgga tgcggatccg
      601 ctcatctccc ccgtccttcg gcggctcggc gtcctttccc ctcgcgtcga aggccggcac
      661 gggctcgtca atcaacggcg gcagcgcgct gagttcgctc caggttttca ccacgggaag
      721 gcgcatcggc gccatttcct cgcttcgacc gaattgggtc aaaagcagcg acacacaaac
      781 cagaatattt ccaagacggc gcatccagcg gggaaaagat tttttgacat tcttataccc
      841 gaaggaatct tgccatgaga aaatcgcaaa tttccaccac gcactgaaga acggcgcttt
      901 ctcccacgaa cactatagta tttctcaaaa gagatttccg aaaagcgatt cattgtaggg
      961 gcagccggag cgaagacagc ctgtcccgcg gaagcgggac agcccgaggg gcgcacgaag
     1021 tgcggcaatt gtgccaacct cccagcgtaa acggggatgc ggcgtccccg tttccccgca
     1081 aacaaaggcg cccaatgggg cggtggggcc ccatttcccc acccgacaag cgcctcaacg
     1141 cattggctat ttcggaaatc ccttttgaga atcgctccag ccgggttccg atgaatcggg
     1201 cactgaggga gcgcgccgaa agccggaatg catgggagac ggcgagccct gtattctgga
     1261 ccaagccaat cccgcgcggg agacgctcga cctcctcacg aacaagcgag tgatccccgc
     1321 cgcttgcccg gcaggcttgt cacaaacgat ccccctcgat tgtctccaga gagtaagcga
     1381 gaatgggtga accgacccgg cccgggccct cccaccgccg gaggagcttg cttcgaccac
     1441 aacaccaccg atcgatgaca aaccagggag tcgaactctt cgaaaaacac cgcccattcc
     1501 tttttggcat cgcctaccgg atgcttggca gcgcggcgga tgcggaggac atggtgcagg
     1561 agacgttcct gcgctggcaa aaaaatgagc ccggcgaact caagtcggcc gaggcgtggc
     1621 tcgccacggt ggtcacgcgg ctgtgcatca accacctcaa atccgcgcgg gtgcagcggg
     1681 aaaaatacgt cggcgcctgg cttccggagc cgatcgtcgc ggactacgcc acggcgaacc
     1741 cgcgggaaaa cgccgagcag gcggaatccc tctcgatcgc gtttctcgtg atcctggaga
     1801 cgctctcccc gacggagcgc gcggtttatc tgctcaagga ggtcttcggc tatgaattcg
     1861 gcgaagtcgc ccgcatcgtc cggaaaagcg agccgaattg ccgccagctc ctcagccgcg
     1921 cccgcgctcg catcgccgag cgccgcccgc gcttccagtc gtcggcggat cagcagcggc
     1981 ggctggtcga ggcgttcttg aaggcgatca atgaggggga cgtggcggga ctcctggcca
     2041 tcctgtccga ggaggtcacc ctcatcaccg acggcggggc ggaaaccccg gcgccccgcc
     2101 ggacgattgc cggcgccgat tccgtttcgc ggatgctcct gcgaggcgct ccgcgcgaag
     2161 cggccgccgc caccgtgacc cggctcgtct ccatcaatgg ctggccgggc ttcctggcgt
     2221 gccgcggcca gacgccgagg gcggcgatcg cgttcgacat cgatggagag cggatccgga
     2281 ccatctacct gatcgcaaat ccggaaaagc tcgcgtcgtt gcccggcggg cggccgaaga
     2341 cggacgcctc cttcgatcgg aattgatccg cccattccgg aaggaatttt gcgggaccgt
     2401 gtcacaaatc cgggcggatc gcggtcttca cttggatgga tatgaaattg gcttccaccc
     2461 ggctcgtgac gaatgacgtc cccgctttgg cgcagtttta ctcggccatc accggtctca
     2521 atccggttgg aatcgaggac tacgttgaac tggatggaaa cctgatcagc ttctttgcct
     2581 catccgccaa accaacccaa cccatcaaaa tatgaaatac aaaaacaccc tctcctggat
     2641 cgtcccggcg ctcattagtt tcttcatgct tttcagcgcg tatatttccg gaacccgcgt
     2701 ggtggaattc gcgcagctcg gcttcccgga atacttccgc atcgagctca ccacggccaa
     2761 agtcatcggc gcgctgcttt tgctcattcc cccggttccc gcgagaatca aggagtggat
     2821 ctacgtgggt ttcggcgtcg tcctcatctc ggccgccatt gcgaaattca gcagcggtta
     2881 tccaatgatc ggagtcctgg agcccttgat cgtatttacc atcatggccg cttcgctcct
     2941 ttacctgaat aagttggcca aaacctaaac caatcctccg gcaccgtcct tgaacatgaa
     3001 cttatttatt cttggcgcca ccggcgccat cggaaaacac ttgttggata tcgcgctgga
     3061 acgaggtcac cgggtgacgg cttacgttcg ctcaccacgg aagatcccgc ttgcccacga
     3121 acgtttgaag gtgatcccgg gggatgtttt taatgccggc cagctggcgc attcgctcgc
     3181 cgggcacgac gccgtcctct cggcgttcgg acccaccacc attcgatctt cgaccctgcg
     3241 gcgcgagttc ggaaggaccc tggcggcggc catgcggaag ggcggcgtcc gtcgggtcca
     3301 gctcgtctcc gcggcctttc tctttcccga catcggtttc ctcggcggac ttttgaaggc
     3361 cacgctgttt cgccggatga ttcccgacat ggcgggaatg gaagccgagg tcagccagag
     3421 tgatctggcc tggaccatgg tccggccgcc ccgcctcacc aatggtccga tctcgggcgc
     3481 ctatcgcatc ctcgatggcg cccttccgcc gggcggattt tatatttccc gggccgatgt
     3541 cgcgcgattc atgatcggcg aagccgaaaa cccggttcac gcgcgacgga tcgtgggttt
     3601 ggccgattaa gagtccgtcg aagaactcct gagctccgcg ttgaccgcgc ctgacccggc
     3661 agcggctaaa tcagtcattc atcccttcgc tccccgggcc gccggcgcgc ggttcgatgc
     3721 cggaacgtcc gtctcgtcga atcgccagag gtcgtcgctt ccgaactcca ggcagcacga
     3781 accgcgcgcc tggacgcatt cggcgcaaag ccactcgccc tcaaaccgaa gggcggcggg
     3841 ccgcccgcac cggtcgcaga ccgcatcctc gtccgccttg tttctcctca tttcatcact
     3901 ccccaaccaa acatccgtcc cggcggcgct gaaggcaaat gccgttcgcc gccgcgccgg
     3961 tggagtgatt tcgcggtctc cagagaaaat caccgccggc cattccggcg aagaatccgg
     4021 atgacctccg cgatcccctc gtcgttgccg atgacgccat ggccgccgcg gacgacgagt
     4081 tccgattcgg cgcccgcgag gtggctgctc ccatagggca cgactccatc cgagcccgtc
     4141 tccttcgggc cggggcggcg ctggccgatc acgctgtgga agggaacctc gaccgggagt
     4201 tccgagagcg cgaggagcgc ggtgctcttc ggggaaagcg tgcgcacggc ggagaaccgc
     4261 ccggcggcat agaactcggc ggctcccggc atcatggccc cgggattggc ccgcgccagt
     4321 tcggtaaagc cgcgttcctc caattgcggc aggcgcgcca ggaaatttcc gagcgttccg
     4381 atccatgaat ccgccatgga actgccccga tgcggagcgg ccatgaagag gacgcgctgg
     4441 acgcgcggat tccgctcaaa gtaaaggatg cgttccagcg cccggacggt ttccggttcg
     4501 ccgctcaatt ggccggtggg aacccgaaac acgctccgcc aaacccggtt tccgcttgaa
     4561 ctcaccagcg tatgggaaat gacgccgccc atgctgtgac cgagcacaac cacctttcgg
     4621 gtcgcggcat cccggttttc cggatcgagg agcttcaccg ttttcccaag cgcctcgcgc
     4681 agcgccgccg cgttggccaa aaccggcgtg ccgctggcgt aatagaattg ccagatttga
     4741 taattcgcgc ggatggccgg atcggcgcgc agcgcgttga cgagcgccgc aaaggccacc
     4801 ggcgtcgatt gcaggccatg caccatgagc agcgggattt tgtccggatc gtagggatcg
     4861 agcagataga tccgcggctt gcgctccatc gatgccggac ggatcatgct cacaaagccg
     4921 gagcgggcga gatgccgggc gcgttttttg agatggtccc ccgccgccgt cggatcgacg
     4981 gcgagc
//
LOCUS       DATDAY010000267         2312 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_1067820, whole genome shotgun sequence.
ACCESSION   DATDAY010000267 DATDAY010000000
VERSION     DATDAY010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2312)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2312)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2312
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1067820"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..231)
                     /locus_tag="VIS74_05305"
     CDS             complement(<1..231)
                     /locus_tag="VIS74_05305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792694.1"
                     /translation="MADFFQTGALATLHRLGATDLAALESRLGEFTRDAPLALVLPCH
                     ARELESPALPLIVRELAAVPYLRQIVVGLDEAD"
     gene            325..1704
                     /locus_tag="VIS74_05310"
     CDS             325..1704
                     /locus_tag="VIS74_05310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792695.1"
                     /translation="MDFPRTLVVLHYHLRPAGVRRVIETALPAIIRAARPHLRRVVVL
                     TGEASPDGWEERLREACGLEPEFRVGKSLNYHPRFVSSPVRQQRLARLIGPDALVWAH
                     NLSVGRNFGLLADLPAACEQAGARLLLHHHDWWFDNRWERVEKRDFARLARLTFPRGS
                     NILHATLTRRECHPLARFLPTLWLPNPVQMAPAASRPDGGWFMPCRVLRRKNILEALL
                     LKSWIAPQSPLRVMGLASSPVEADYHARIAALGQVEFGAGFKPDGPLAVTSLLEGFGL
                     PFLETAAAGRPFIGRYLANASPDLRRAGCSLPLLYREIRMSRDWFDFAAETARQRSLW
                     KQWRSALPAAARRLALPPRFSGKGASFSRLTLTAQLEVISSRPAWESAAKGNPVLEKA
                     VESGELPAASLTEGARDFFSAETYAQRFWKGVGARRISPSRDGAFAAMLQQKMAADNL
                     YPLLMAEIT"
     gene            1701..>2312
                     /locus_tag="VIS74_05315"
     CDS             1701..>2312
                     /locus_tag="VIS74_05315"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEY5792696.1"
                     /translation="MTPTPLPRAVVFDIYGTLLKIAPLARSEAEWRQAFGMTREDFQV
                     RVRQEIFRRHEIARAAGIAYPEIQWPEVVQAVAPGMSPALAARFERRTEAQAGARELL
                     GWLRRRALPLGLVSNAQAYTREELRECGIEPEWFPIAFLSYEQGFSKPDPLAFRWLAG
                     KFAPIPPAEILVVGDRADNDILPALAAGFAAWQVTDAGLNPLLD"
BASE COUNT          363 a          755 c          779 g          415 t
ORIGIN      
        1 gtcagcttcg tccaggccga ccacgatctg gcgcaggtag ggaaccgcgg ccagttcgcg
       61 cacgatgagc ggcagggccg ggctttccag ctcgcgggcg tggcagggca gcaccagggc
      121 cagcggcgca tcgcgggtga attcccccag gcggctttcg agcgcggcca gatcggtggc
      181 gccgaggcgg tggagcgtgg cgagcgcgcc ggtttggaag aagtcggcca tgggaagaga
      241 agaatcatca cacgggtcgc ggcgagcaca gcgaaatatt taggaattga ttccccccgg
      301 ccgccggggt gtgctcaggg gttcatggat tttccccgca cgctggttgt cctgcattac
      361 catctccgcc ctgccggcgt gcggcgggtg atcgaaacgg cgctgccggc gatcatccgc
      421 gccgcccggc cccatttgcg gcgggtggtg gtgctgacgg gggaagcctc gccggacgga
      481 tgggaggagc ggctgcggga agcctgcggg ctggagccgg agtttcgcgt gggaaaatcg
      541 ctcaattacc atccgcgctt cgtgagttcc ccggttcgcc agcaacggct tgcgcggctc
      601 atcggcccgg acgcgctggt ctgggcgcac aacctcagcg tggggcggaa ttttggattg
      661 ctcgcggacc tgcccgccgc gtgcgaacag gcgggcgcgc gcctgttgct gcatcaccac
      721 gactggtggt tcgacaatcg ctgggagcgc gtggaaaaaa gggattttgc ccggctggcc
      781 cggctgacgt ttccccgcgg ttcgaacatc ctgcacgcca cgctcacccg gcgggaatgc
      841 catccgctgg cccggttttt gcccacgctc tggctgccga atccggtgca aatggcgccc
      901 gccgcttccc gcccggacgg cggctggttc atgccctgcc gggtgctgcg gcgcaaaaat
      961 attctggaag cgctgctgct gaaaagctgg atcgcgccgc aaagcccgtt gcgggtgatg
     1021 ggactggcca gctcgccggt cgaagcggat tatcatgccc gcatcgccgc tctgggacag
     1081 gtcgagttcg gcgccggctt caaacccgat ggacccttgg cggtaacctc gctgctggaa
     1141 ggtttcgggc ttcccttcct cgaaaccgcc gccgccggcc ggccgttcat cgggcgttat
     1201 ctggcgaacg cctcccccga tctccggcgc gcggggtgtt cacttccgct gctttatcgg
     1261 gaaatccgga tgagccggga ttggtttgat ttcgccgccg aaaccgcgcg ccagcggtcg
     1321 ctctggaaac aatggcggtc ggccctgccc gccgccgccc gccgtctggc gcttccgccg
     1381 cggttctccg ggaagggcgc gtccttcagc cgcctcaccc tcacggcgca gttggaagtg
     1441 atctccagcc gtcccgcctg ggaatcggcg gcgaaaggga atccggtttt ggagaaagcg
     1501 gtggagagcg gcgaattgcc cgccgccagc ctgacggagg gcgctcgcga ttttttttcc
     1561 gcggaaacct acgcgcagcg attttggaag ggcgtggggg cgcggcggat ttcgccatcc
     1621 cgggacggcg cgtttgcggc gatgctgcaa caaaaaatgg cggcggataa tttgtatccg
     1681 ctgttaatgg cggagataac atgacaccaa ccccacttcc ccgggcggtc gttttcgaca
     1741 tctacggcac gctgctgaaa atcgcccccc tcgcccgttc cgaagcggag tggcggcagg
     1801 cattcggaat gacccgcgag gacttccaag tccgcgtccg ccaggaaatt ttccgccgcc
     1861 atgaaatcgc ccgcgcggcc ggcatcgcat atccggaaat tcagtggccg gaagtcgtgc
     1921 aggccgtcgc tcccggcatg tcccccgccc tcgccgcccg ttttgaacgg cggacggaag
     1981 cccaggcggg cgcgcgcgaa ttgctcggct ggctgaggcg gcgcgccctt ccgctcggcc
     2041 tggtttccaa cgcgcaggct tacacgcggg aggaattgcg cgaatgcggc atcgaacccg
     2101 aatggttccc catcgccttt ctttcctacg aacagggctt cagcaaaccc gacccattgg
     2161 cgtttcgctg gctggccgga aaattcgcgc ccattcctcc ggcggaaatc ctcgtcgtgg
     2221 gcgaccgggc cgacaacgac atcctccccg cgctggccgc cggattcgcc gcctggcagg
     2281 tcaccgatgc gggattgaat ccgctgctgg at
//
LOCUS       DATDAY010000268         9078 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_2338224, whole genome shotgun sequence.
ACCESSION   DATDAY010000268 DATDAY010000000
VERSION     DATDAY010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 9078)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9078)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9078
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2338224"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..379
                     /locus_tag="VIS74_05320"
     CDS             <1..379
                     /locus_tag="VIS74_05320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HEY5792697.1"
                     /translation="LKFENELIFDGKIEGEIVSEGALTLGKNARVQGEVRTKTVTVHG
                     TVTGNITVTEKCELKASSQLTGDLRATRIIIEEGATFVGKSEVTPSKQVSQRETPSSL
                     TSTSASSVTDRSKTPALAASPQG"
     gene            477..1262
                     /locus_tag="VIS74_05325"
     CDS             477..1262
                     /locus_tag="VIS74_05325"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HEY5792698.1"
                     /translation="MARRIRKISADCPKCGHRQSEPEDFISTLCQGCGETFHQSPRPP
                     GRPSAAPLFKRPPRLIHCHRCGRLHEASPFAEATSCPQCNSRIQLGPVRIESHASRVI
                     DTRGDLTIGPEGYLNTTATYCTNATIHGRISGFLLCEKTLTLDMKGRCQARIEARSVI
                     IERHAELSFYFPIRTHELIVRGRASAVVHCSGGVWVKKTGQLEGEIRAHSFQVDKGGR
                     FQGRLAVNAPEISPEAFSSPQREFPFLAIGKRGQEFNPDYTTT"
     gene            complement(1301..1933)
                     /locus_tag="VIS74_05330"
     CDS             complement(1301..1933)
                     /locus_tag="VIS74_05330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792699.1"
                     /translation="MSNLPVENPSDDLSIGGLPADLFWEKYKGALLGGIAAVTVIAAG
                     VLAWLLHSHNERVSSEARFAEARTPEEYRAVIEKYPGTPVAGNASLLLAAAQRPNLKE
                     STATYEALLAAAPKYPLRSSAALGLAANAELANDPSKAGELYQQAALAHQDSYGAPFA
                     LYAAAELQLREGKRAEALKTFRNLAGAYSATVSGGFARMQAERLSSLEAP"
     gene            complement(2029..2994)
                     /gene="cysK"
                     /locus_tag="VIS74_05335"
     CDS             complement(2029..2994)
                     /gene="cysK"
                     /locus_tag="VIS74_05335"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: protein motif:HMM:TIGR01136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HEY5792700.1"
                     /translation="MGKIYNNIVETIGRTPLVKLNHVTQDLPATIALKCEFFNPLGSV
                     KDRIGMAMIEDAEKRGILTKETVIVEPTSGNTGIALAFVAAAKGYKLILTMPETMSLE
                     RRTLLAMLGAKLVLTPGAEGMRGAISRAEQIVKETPGAWMPQQFTNPANPEIHKKTTA
                     EELWEDTDGKIDILVAAVGTGGTITGASEVIKARRPGFKAIAVEPKDSPVISQTLSGE
                     PVKPGPHKIQGTGAGFVPANLHLDAVDEVVTVTNDEAFAMARRLAKEEGLLVGISTGA
                     NVHAAIQVAQRPENAGKLIVTVACSTGERYLSTALAEEARAQVGG"
     gene            3081..3359
                     /locus_tag="VIS74_05340"
     CDS             3081..3359
                     /locus_tag="VIS74_05340"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006984006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HEY5792701.1"
                     /translation="MAQLLNPDEIAAAATRVEAWEVKGNALERTFRFKDFRGALAFVN
                     RVGELAEAANHHPDIDIRWNKVRLELSTHSAGGLTALDFDLAGQINAL"
     gene            complement(3369..4457)
                     /locus_tag="VIS74_05345"
     CDS             complement(3369..4457)
                     /locus_tag="VIS74_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HEY5792702.1"
                     /translation="MAYIDQFFSVLVESGASDLHLAVGRSPKIRYHGDIVSIREEPLT
                     EEEMAYMLSEISGPDRWNRFLAKGDLDFAYEMSARARFRCNYLKQASGYGAVFRLIPT
                     KIATLDQLGIPEVVKKFANVRGGLVLVTGPTGSGKSTTLAALIDYINSNYTKHIITVE
                     EPIEFVHDHKRSIITQREVPEHAASFAAGLKAALREDTDIVLVGEMRDLETISLALTA
                     AETGLLVFGTLHTNNARKTVDRLVDVFPADQQSQVRTMLSASLRGVVAQLLLKKQDGS
                     GRVAVNEILVNTPAVASIIREGATQKLQDVITGGRAEGMQFMDDAIWKELVGGTVSPH
                     EAFMKAIDKQRFKSYLPPEESELANSGG"
     gene            complement(4473..5567)
                     /locus_tag="VIS74_05350"
     CDS             complement(4473..5567)
                     /locus_tag="VIS74_05350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HEY5792703.1"
                     /translation="MSHPADSPALTTEHVDPYLEMGTSSGASDVHLGAKCQPTWRRFG
                     VLEPIWKEAPFLTAQDTERMTQNLLTEEQAKKLATTGDVDFAYSTSFGRFRGSVVRQR
                     HGYELVFRIINAVVRTMEELGLPEHLKQLTQYHNGLILVTGAVGSGKSTTLAAFIEQV
                     NRTRKDHIITLEDPIEFIYEPKGCQITQREVHLHTESFSAALRGCLRQDPDVLLVGEM
                     RDLETISLAVTASETGHLVLGTLHTSSAARTLDRVLDVFPLDQRDQIRIMVSESLRGI
                     VTQQLIPRADGKGRVMAMEVLTNSPAVANCIREGKTFMLPGVMQTGKKLGMKLMDESL
                     MDLYDKGMISEVEAEARAEDKLRMRQHLKR"
     gene            complement(5646..8159)
                     /locus_tag="VIS74_05355"
     CDS             complement(5646..8159)
                     /locus_tag="VIS74_05355"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY5792704.1"
                     /translation="MSDPALLPIKVRAKFFFEGERKFFLKGSTYGPFPPDEKGEFFGN
                     PEKARKDLGMIREAGINLLRVYYVPPRWFLDLCAEHGVRLLISIPWPQNVSFLDDPKI
                     RRGIFKTVREGVLANLGHPAIFGFLMGNEIDTTMIRWLGARKVTEFLETLLILCKELN
                     PGVLYSYASYPPTEYLLPQNVDFYTFNVYLHRREDFERYLARLQNLAEDRPLVLGEFG
                     MDTIRHGQDEQAEFLHWQIQSSVRGGAAGAFVYSWTDEWFRGGVEILDWAFGLTTRER
                     VPKKAYFTVKALFGGAAPIIEKAELPSYPKVSVIVCSYNGAKTLDNCLESLKHLNYPN
                     YEVVLVDDGSKDNTPEIAAKHPWVKNIRQENKGLSFARNVGAYASTGEIIAYTDSDCM
                     ADPDWLYYLAGTLLSGDYAGVGGPNISPPAQDWIQACVAAAPGGPSHVLLTDVVAEHI
                     PGCNMAFHRWAFERVGGFDAEYRKAGDDVDFCWRLQQDGGVIAFSPAAIVWHYRRFTL
                     DAFRKQQQGYGEAESMLRFKHLIFFGPTGTAKWKGQIYGAPRFTWLVNRPVIYHGVFG
                     SGLFQSIYPTPQSEIAAYLSSIEWVTLTAFIAAISVPIPALRVVPYLMFAGTFLVALS
                     YMIHAKLEPQFDTIRARLLVAFLALTQPLFRGWTRYETWLKYKYTPSAVIASPEKDAQ
                     RIKNPTHLEFWSGEWRTRDNLLHQTIQLLEEEGWNYSTDTGWKRWDIQVYGSFWWGVE
                     MHTVTEIHSGGQLTRVLLKTRCVATTVLINLILIGLLAYNVFFAPWLAVLLALPYAGF
                     VAYLAYRARGLKERVAQLVHAAARQCGFVLPKKTETEKK"
     gene            complement(8255..8638)
                     /locus_tag="VIS74_05360"
     CDS             complement(8255..8638)
                     /locus_tag="VIS74_05360"
                     /inference="COORDINATES: protein motif:HMM:TIGR04256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HEY5792705.1"
                     /translation="MDTDLHGLRYERETREIIGAAIEVLNMLGHGLLEKPYENALAVE
                     FRLRNLPFVQQPRFNVIYKGTIVGDYVPDLIVANRVVVDTKVIEGITNHDLGQMLNYL
                     KITQLPVGLILNFKRSRLEWKRAAL"
     gene            complement(8650..>9078)
                     /locus_tag="VIS74_05365"
     CDS             complement(8650..>9078)
                     /locus_tag="VIS74_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEY5792706.1"
                     /translation="EIMDWLAPTGPVYQAGTLSGNPLALAAGLAQLRELERVNGWQKL
                     EEIGRELEAAVRPLIKRRPFVWKRIGSMFCLYFTAGPVYDLADAKQSDTAAFGRFFHR
                     CLDAGVYFAPSQFEAGFLSLAHDSAALEATAKVVAEALAN"
BASE COUNT         1661 a         2833 c         2855 g         1729 t
ORIGIN      
        1 gctgaaattt gagaacgaat tgatctttga cggaaaaatc gaaggcgaaa tcgtgtcgga
       61 aggcgcgctg acgctcggca aaaacgcccg ggtccagggc gaagtccgca ccaaaaccgt
      121 gacggttcat ggcacggtca cgggcaacat caccgttacc gaaaagtgcg agctcaaggc
      181 cagctcccag ctcaccggcg acctgcgcgc cacgcgcatc atcatcgagg aaggcgccac
      241 cttcgtcggc aaatccgagg ttaccccgag caagcaggtc agccagcgcg aaacgccttc
      301 gtctttgacc tcgacttccg cttccagcgt gacggatcgt tccaagaccc ccgcattggc
      361 ggcgagcccg cagggttgag ccgccttgcc cggcccggct catttcgttc gcatggatgt
      421 ttccgctacc gaatggataa gagcccttga ccggcggcag gcggatcggg gctgatatgg
      481 cacggcgcat ccggaaaatc tccgcggatt gtccgaaatg cggccatcgc caatccgaac
      541 cggaagactt catctcaacc ctctgccagg gatgcggcga gacctttcac cagtctcccc
      601 gcccgccggg gcgcccctcg gccgcgccgc ttttcaagcg ccccccgcgt ttgatccatt
      661 gccaccgctg cggccgcctc cacgaggcct cgcccttcgc ggaggccacg tcctgccccc
      721 agtgcaactc gcgcatccag ctcgggcccg tccggatcga atcccacgcg tcgcgggtga
      781 tcgatacgcg gggcgatctt accatcggcc cggaggggta tctcaacacg accgcgacgt
      841 attgcacgaa cgcgaccatt cacggccgca tttcgggatt tctcctctgc gaaaagacgc
      901 tcacgctgga tatgaaagga cgctgccagg cgcgcatcga ggcccggagc gtcattattg
      961 agcggcacgc ggagttgtcg ttctattttc ccatccgcac ccacgagctc atcgtccggg
     1021 ggcgcgcctc cgccgtcgtt cattgttccg gcggggtgtg ggtcaagaag accgggcagc
     1081 tcgaggggga gattcgggcg cactcatttc aggtggacaa aggcggccgc ttccagggcc
     1141 ggctggcggt gaacgccccg gaaatttccc cggaggcatt ttcctcgccg cagcgggaat
     1201 ttccgttcct ggccatcggg aaacgcggcc aggaattcaa tccggactac acgacgacgt
     1261 agggcggggg agcgtttcgc cgaggcgccg ctcccgaagg ttacggagcc tccagggagg
     1321 agaggcgctc cgcctgcatg cgggcaaacc cgccggagac cgttgcggaa taagctcccg
     1381 cgagattgcg aaaagttttc agcgcctccg cgcgcttgcc ttcgcgcaac tgcaactccg
     1441 ccgcggcata aagcgcaaag ggagccccgt aggaatcctg gtgagccaag gccgcctgct
     1501 ggtaaagttc cccggccttg gacgggtcat tggccagttc cgcgttggcg gccagcccca
     1561 atgcggcgga tgaccgcagc ggatatttgg gggccgccgc cagcaacgcc tcatacgtcg
     1621 cggtggattc cttcaggttc gggcgctggg cggccgccag cagcagcgag gcatttccgg
     1681 ccaccggggt gccggggtat ttttcgatga cggcccggta ttcctccggc gtgcgcgcct
     1741 ccgcaaaccg ggcttcggaa cttacccgct cgttatgcga gtgcagcagc caggccaata
     1801 cgcccgcggc gatgaccgtc acggcggcaa tgccgccgag gagggctccc ttgtattttt
     1861 cccaaaacaa atccgcgggc aatccgccga tgctcagatc gtcggagggg ttttcaacgg
     1921 gcaaattcga catggccatt ttttagaatg cgaaatggaa aatgcgaagt gcgaaatggg
     1981 gagacgccaa tttcgcattc cccatttccg catttcgaat tcaagcgatc agccgccgac
     2041 ctgggcgcgg gcttcctccg cgagcgcggt gctcaggtaa cgctcgccgg tggagcaggc
     2101 gacggtcacg atcagcttgc cggcgttttc cggacgctgg gcgacctgaa tggcggcatg
     2161 cacgttcgcg ccggtgctga tgcccacgag caggccttcc tccttcgcca ggcggcgggc
     2221 catggcaaag gcttcgtcat tggtcaccgt gacgacttcg tccacggcgt caaggtgcag
     2281 attggccggc acgaagcccg ctccggtgcc ctggatcttg tgcgggcccg gcttgacggg
     2341 ttcgccgctg agcgtctggg aaataaccgg ggaatccttg ggctccaccg cgatggcctt
     2401 gaagccgggc cggcgggcct tgatgacttc ggaggcgccg gtgatcgttc cgcccgtgcc
     2461 cacggcggcg acgaggatgt cgatcttgcc gtccgtgtcc tcccacagct cctccgcggt
     2521 ggtttttttg tgaatctccg ggttggccgg gttggtgaat tgctgcggca tccaggcgcc
     2581 cggcgtttcc ttgacgatct gctccgcgcg ggaaatggct ccgcgcatgc cttcggctcc
     2641 gggcgtgagc accagcttcg cgccgagcat ggcgagcagc gtgcggcgct ccaggctcat
     2701 cgtttccggc atggtcaaaa tcagcttata ccccttcgcc gccgccacga aggccagcgc
     2761 gatgcccgta tttccgctcg tcggctccac gatcaccgtc tccttggtga gaatcccgcg
     2821 cttctcggcg tcttcgatca tcgccatgcc gatgcggtcc ttcacgctgc ccagcgggtt
     2881 aaagaactca cacttcagcg cgatggtcgc cggcaggtcc tgggtgacgt ggttgagttt
     2941 taccaaaggc gtgcgcccga tggtttcgac gatgttattg tagattttgc ccatgggatt
     3001 ggtcgggtat gagattggag gttttcctgc attacacact tccacttgcg agcgcaaatc
     3061 ggaagatgca aattttaggt atggctcagc tgttaaatcc ggacgaaatc gccgccgccg
     3121 ccacccgggt ggaagcgtgg gaagtcaagg gcaacgccct ggagcggacg tttcgcttca
     3181 aggacttccg gggagccctg gcgtttgtca accgggtcgg ggaactcgcc gaagcggcca
     3241 atcaccatcc ggacatcgac atccgctgga acaaggtccg gctggaattg agcacgcaca
     3301 gcgcgggcgg cctcacggcg ctggatttcg acctcgccgg ccaaatcaat gcgctttgac
     3361 cgggcggatt aaccgccgga gttggcaagc tccgactcct cggggggcag gtaacttttg
     3421 aaacgctgct tgtcgatggc cttcatgaag gcctcgtgcg ggctgaccgt gccgcccacg
     3481 agttccttcc agatggcgtc atccatgaat tgcatgccct ccgcccggcc gccggtgatg
     3541 acgtcctgca gcttctgggt cgcaccctcc cggatgatgg aggccaccgc cggcgtgttg
     3601 accaggatct cgttgaccgc gacccggccg gagccgtcct gctttttcaa gagcaattgg
     3661 gccaccaccc cgcgcaggga ggcggaaagc atggtgcgaa cctgggattg ctgatcggcg
     3721 ggaaagacgt ccaccaggcg gtccaccgtc tttcgcgcat tgttcgtatg cagggtgcca
     3781 aaaaccagca ggccggtttc cgcggcggtg agcgccaggg aaatcgtttc caggtcgcgc
     3841 atttccccca ccagaacgat gtccgtgtcc tcgcgcagcg ccgccttcag gcccgcggcg
     3901 aatgaggcgg cgtgctcggg cacctcgcgc tgcgtgatga tgctgcgctt gtgatcgtgg
     3961 acgaactcga tcggctcctc cacggtgatg atgtgcttgg tgtagtttga gttgatgtaa
     4021 tcgatcagcg ccgccagcgt ggtgcttttg cccgagccgg tggggccggt cacgagcact
     4081 aggccgccgc ggacattggc gaattttttg accacttccg ggatcccgag ctggtcgagc
     4141 gtggcgatct tggtgggaat caaccggaac accgcgccgt agcccgaggc ctgcttgagg
     4201 tagttgcacc ggaagcgcgc gcgggcgctc atctcgtagg cgaaatccag gtcgcctttg
     4261 gccagaaaac gattccagcg gtcggggccg ctgatctcgc tgagcatgta agccatctcc
     4321 tcctccgtca gcggctcctc gcggatcgat acgatgtcgc catgataccg gattttgggc
     4381 gaccggccga ccgccaggtg gagatccgag gcgccggatt ccacgagcac actaaaaaat
     4441 tgatcaatgt aagccatggg agtttgccgt tgctaccgtt tcaagtgctg gcgcatccgg
     4501 agtttgtcct cggcccgggc ctcggcttcg acctccgaga tcatgccttt gtcatacaaa
     4561 tccatcagcg attcatccat caacttcatc ccgagttttt tgccggtctg catgacgccg
     4621 ggaagcatga acgtttttcc ctcccggatg cagttggcca ccgcgggtga attggtcagc
     4681 acctccatgg ccatcacccg gcccttgcca tcggcgcggg gaatgagctg ctgggtgacg
     4741 atgccgcgca gggattcgct gaccatgatg cggatctggt cgcgctgatc gagcgggaag
     4801 acgtcgagga cgcggtccag ggtgcgggcg gcgctgctgg tgtgcagggt tcccaggacg
     4861 aggtggccgg tttcggacgc ggtgaccgcg aggctgatgg tttccagatc gcgcatttcg
     4921 ccgacgagca gcacgtccgg atcctggcgc aggcagccgc gcagggcggc ggaaaacgat
     4981 tccgtgtgga gatggacctc gcgctgcgtg atctggcatc ccttcggctc gtaaatgaac
     5041 tcgatcggat cctcgagggt gatgatgtga tctttgcgcg tccggttcac ctgctcgata
     5101 aaggcggcca gggtggtgga cttgccgctg ccgacggcgc cggtgacgag gatgaggccg
     5161 ttgtgatact gggtgagctg cttgagatgc tccggcagcc ccagctcctc catggtccgg
     5221 accacggcat tgatgatgcg gaaaaccaat tcgtagccgt gccgctggcg cacgacgctg
     5281 ccgcgaaacc ggccgaagga agtggaatag gcaaaatcca cgtcgccggt cgtggcgagt
     5341 ttttttgcct gctcctcggt cagcagattt tgcgtcatcc gctccgtatc ctgcgcggtc
     5401 aggaacgggg cttccttcca gatcggttcc agaacgccga agcgccgcca ggtcggctgg
     5461 cacttggcgc ccaggtggac atccgaggcg ccggaggacg ttcccatttc cagataggga
     5521 tcgacgtgct ccgtcgtgag ggcgggggaa tcggcggggt ggctcacagc gccaccacta
     5581 tgcaagaatg gcgaaagcgc tcaactcgaa agacacgctt cgcgcggaac gattcgccgc
     5641 gaggcctatt ttttctccgt ctcggttttt ttcggcagca cgaacccgca ctgccgggcc
     5701 gccgcgtgga ccagctgcgc gacgcgctcc ttcaagccgc gcgcgcggta ggcgaggtag
     5761 gccacaaatc ccgcgtaggg cagcgccagc aggacggcca gccacggcgc gaagaaaacg
     5821 ttgtaggcca gcaggccgat caggatcagg ttgatcagga ccgtcgtcgc cacgcagcgc
     5881 gtcttcaaga gcacgcgggt gagctggccg ccgctgtgaa tctcggtgac cgtgtgcatt
     5941 tccacgcccc accagaagct gccgtaaacc tggatgtccc agcgcttcca gcccgtgtcg
     6001 gtggagtaat tccagccctc ctcctcgagc agttggatcg tttgatgcag caggttgtcg
     6061 cgcgtgcgcc attcgccgct ccagaattcc agatgcgtcg ggttcttgat gcgctgggcg
     6121 tccttttccg gactcgcgat gaccgcgctg ggcgtgtatt tgtatttcag ccacgtctcg
     6181 tagcgggtcc agccgcggaa gaggggctgg gtcaaggcca gaaacgccac gagcaggcgc
     6241 gcgcgaatcg tgtcgaactg cggctccagc ttcgcgtgga tcatgtaaga cagcgcgacc
     6301 aaaaacgtgc cggcaaacat cagatacggc accacgcgca gcgccggaat cggcaccgaa
     6361 atcgccgcga tgaacgcggt gagcgtcacc cattcgatgc tgctcagata cgccgcgatt
     6421 tccgactgcg gggtgggata aatggactgg aacaaaccgc tgccgaacac gccgtggtag
     6481 atcaccgggc ggttcaccag ccacgtgaag cgcggcgcgc cgtagatttg ccccttccac
     6541 ttcgccgtgc cggtggggcc gaagaaaatg aggtgcttga agcgcagcat ggactccgcc
     6601 tcgccgtagc cctgctgctg cttgcggaag gcgtcgaggg tgaagcggcg gtagtgccac
     6661 acgatggccg ccgggctgaa ggcgatcacg ccgccgtcct gctgcaggcg ccagcagaag
     6721 tccacgtcgt cgccggcttt gcggtattcc gcgtcgaatc cgccgacgcg ctcaaacgcc
     6781 cagcggtgaa acgccatgtt gcagccggga atgtgctcgg ccacgacgtc ggtgagcagc
     6841 acgtggctgg ggccgccggg cgcggcggcc acgcaggcct ggatccaatc ctgcgcgggc
     6901 ggcgaaatgt tcggcccgcc cacgccggcg tagtcgccgc tgagcagcgt gccggccagg
     6961 taatacagcc agtccggatc ggccatgcag tcggaatccg tgtaggcgat gatctcgccg
     7021 gtcgaggcat aagcgccgac attgcgcgcg aagctgaggc ccttgttttc ctggcggatg
     7081 ttcttcaccc acggatgctt cgcggcgatt tccggcgtgt tgtccttcga gccgtcgtcc
     7141 acgagcacga cttcgtaatt ggggtaattc aggtgcttga gcgactcgag gcagttgtcg
     7201 agcgtcttcg cgccgttgta agagcagacg atgaccgaga ccttcggata cgagggcagc
     7261 tcggcttttt caatgatggg cgccgcgccg ccaaagagcg ctttcacggt gaaatacgcc
     7321 ttcttcggca cgcgctcgcg cgtcgtcagg ccgaaggccc agtcgagaat ctccacgccg
     7381 ccgcggaacc attcgtcggt ccaggaatag acaaacgccc ccgccgcgcc gccgcgcacg
     7441 ctgctctgga tttgccaatg caggaattcg gcctgttcgt cctgcccatg gcggatggta
     7501 tccattccaa attcgccgag caccagcggg cggtcctccg cgaggttctg caggcgggcg
     7561 aggtaacgtt cgaagtcctc gcggcggtgc agatagacgt tgaacgtgta gaagtccacg
     7621 ttctgcggca ggaggtattc cgtcggcgga taactcgcgt aggagtagag cacgccggga
     7681 ttcagctcct tgcacaggat gagcagggtt tcgaggaatt cggtcacctt ccgcgcgccg
     7741 agccagcgga tcatggtggt gtcgatctcg ttgcccatca ggaatccgaa gatcgccgga
     7801 tgccccaagt tcgccagcac accctcgcgg acggtcttga agatgccgcg gcggattttc
     7861 ggatcgtcga ggaaggaaac gttttgcggc cagggaatgg aaatcagcag ccgcacgccg
     7921 tgctccgcgc agaggtcgag gaaccagcgc ggcggcacgt aataaacgcg cagcagattg
     7981 atgcccgcct cgcgaatcat gcccaggtcc ttgcgggcct tttcgggatt gccgaaaaac
     8041 tcgccctttt cgtccggagg gaacggtcca taggtggagc ctttcaggaa aaatttccgc
     8101 tcgccttcga agaaaaactt ggcccgaacc ttgatgggca ataacgcggg gtctgacatg
     8161 gatgtgaacc gttacgcgtt actggaaggc gcggaccttg tcgagccggt ttggtgaacc
     8221 acggatggac acggatccac acggataaaa cggatcaaag ggcggcgcgc ttccattcca
     8281 aacgggagcg cttgaaattg aggataagtc ccacgggaag ctgggtgatt ttaaggtagt
     8341 tcaacatctg gccaagatcg tggttggtga tgccttcgat gactttcgtg tccaccacca
     8401 cccggttcgc cacgatcaaa tcgggcacat agtccccaac aatcgtgcct ttataaatca
     8461 cgttaaatcg cggttgctga acgaatggaa ggtttctcag tcgaaactcc accgccagcg
     8521 cattctcata aggtttctcc agcaacccat gcccgagcat attaagcacc tcgatcgccg
     8581 ccccgatgat ctcccgggtc tcccgctcat atcttaatcc gtgtaaatcc gtgtccatcc
     8641 gtggttcatt cagttcgcca gcgcctccgc cacgaccttg gccgtcgctt ccagcgccgc
     8701 ggaatcgtgc gcgagcgaca ggaagcccgc ctcgaattgc gagggcgcga aataaacccc
     8761 cgcgtccagg cagcgatgga aaaagcgccc gaacgccgcc gtgtcgctct gcttcgcgtc
     8821 ggccagatcg tacaccggac ccgccgtgaa atagaggcag aacatcgagc cgatccgttt
     8881 ccagacaaaa ggccggcgct tgatcagcgg ccgcaccgcc gcctccagct cccgcccgat
     8941 ttcctccagc ttttgccatc cgttcacccg ctccagctcc cgcagttgcg ccaggccggc
     9001 ggccagcgcg aggggatttc ccgaaagcgt ccccgcctga tacaccggcc cggtcggcgc
     9061 gagccagtcc atgatctc
//
LOCUS       DATDAY010000269         3375 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_2161774, whole genome shotgun sequence.
ACCESSION   DATDAY010000269 DATDAY010000000
VERSION     DATDAY010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 3375)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3375)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3375
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2161774"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..920)
                     /locus_tag="VIS74_05370"
     CDS             complement(<1..920)
                     /locus_tag="VIS74_05370"
                     /inference="COORDINATES: protein motif:HMM:NF038287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Amuc_1100 family pilus-like protein"
                     /protein_id="HEY5792707.1"
                     /translation="MNWTKENPFLAGFIAISLLVVGGLGYYAYTNYTAYTELRSSYET
                     EVGTLHTFQNRTPFPSKENLQKIIESQKSYHTALLGLRKSLEAHELPLNPKISPQQFQ
                     DELRRAVSLAQSKAAQARMALPPDFYLGFDVYSTSLPRPEATPYLDRELKVIEALFAG
                     LIDAGVESLNQFVRTPLAVESGSAGKAAAPAKPGAGNNDAAGSKMVEKHLLDLTFTGE
                     QGRVRLAVNSLLNSPQFAVVRALKFQNSSPQSPPKLLETALPNDPEPAPGRTASKALS
                     MVFGREKITAQVRLELLSFEKIDPPRAEKS"
     gene            complement(917..2683)
                     /gene="pilM"
                     /locus_tag="VIS74_05375"
     CDS             complement(917..2683)
                     /gene="pilM"
                     /locus_tag="VIS74_05375"
                     /inference="COORDINATES: protein motif:HMM:NF022554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus assembly protein PilM"
                     /protein_id="HEY5792708.1"
                     /translation="MAAPTKYYALNLGMQTISLAEFHATAGGGLILHAFKQTELIVDP
                     SADATRSEQVRLAVEELRTALRVKKGETVNYALPSQSVFTRFVRLPGANAEDVRSVIG
                     FEAQQNVPFPIDEVVWDYQIMGKPRDDQWDVVLVAIKSDQLTNLNAAVEAAGLKAGYI
                     DVAPMAIYNAFRFNQSDAAGTSLLIDIGSRTTNLIFIEGQKVFSRSIPIGGNTISAAI
                     AKELGEEITFAEKLKLEKGFVGLGGAYAEPDDAMEAKISKLVRNTLTRLHAEITRSIS
                     FYRANQGGTQPVRAVLCGGTVSFPYMAEFFSEKLQFPVEFLNPLRNVTAANEAVASEV
                     AGKTHTLGELVGLGLRNLRNCPMEINLRPASVIRQQDLARRKPYLIAALVTVSFALIA
                     TSLYFQRASEILADVVQKVQSDTNRLKQLAAQTEAALAERGKLEKAAAPLLLAIDERV
                     IWNTLLDELGAYLPPKFIWITQLTPLSQNGPVAIEPGKMLGSGASSGPVAPAKPGTPP
                     PKPATIDAIELQGLYLDIPENAQQAVIIDTFVENLTASGLFEIDPENKAKYVLQRTQP
                     DGQSWAYGYTLRLPLKKPISLQ"
     gene            complement(2717..>3375)
                     /locus_tag="VIS74_05380"
     CDS             complement(2717..>3375)
                     /locus_tag="VIS74_05380"
                     /inference="COORDINATES: protein motif:HMM:NF042424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Amuc_1102 family pilus-like protein"
                     /protein_id="HEY5792709.1"
                     /translation="MNPLLPVVVLTTLSLLPASAQLAQGDLVEVTGVTVDYVTTPEFS
                     FQFGPKGKKTKNREWLEIETTFNWQPRPSPSAPRFLDELTVNYYILLNNPGFRDPSTG
                     GLAKATLLTGRSTLTAVAAGKGLKTVVYISPRTLDRFFDGKQPATAGSAVKEVSVAVT
                     RGGQLVGYLSQGKFPPNWWNDFQPNVQGYVLNKNETPFAAVAWDYYEPLKAQQTPGGF
                     "
BASE COUNT          636 a          923 c         1086 g          730 t
ORIGIN      
        1 ttggatttct ccgcgcgcgg agggtcgatt ttttcaaagc tcaggagctc aagccggacc
       61 tgagccgtga ttttttcccg tccaaacacc atgctgagcg ccttggaagc ggtccggccg
      121 ggagcgggtt ccggatcgtt cggcaaggcg gtttcaagaa gcttgggggg gctttgggga
      181 gaactgtttt gaaattttaa cgcgcggacg acggcaaatt gcggagagtt cagcagggag
      241 ttcaccgcca ggcgcacgcg gccctgttcg ccggtgaagg tgagatccag caagtgcttt
      301 tcgaccattt tcgagccggc cgcgtcatta ttgccggctc caggtttggc gggggcggcg
      361 gctttgcccg cgcttccgct ttccacggcc agcggcgttc ggacgaactg attgaggctt
      421 tccacccccg catcgatcag cccggcaaag agggcttcga tcaccttgag ttcacggtcc
      481 agatacggtg tggcttccgg acggggcagg ctggtgctgt aaacatcgaa cccgaggtaa
      541 aaatcgggcg gcaacgccat gcgggcttgg gccgccttgg actgcgcgag cgacaccgcc
      601 cggcgaagtt cgtcctggaa ctgctgcggg cttattttgg ggttaagggg cagttcatgg
      661 gcctcaaggg attttcgcag gcccagcaag gcggtgtgat aggatttctg cgactcgatg
      721 attttttgca ggttttcctt gctcgggaac ggcgtccgat tctgaaacgt atggagcgtg
      781 cccacctcgg tttcgtagct ggatctgagt tccgtgtaag cggtgtaatt ggtataggcg
      841 taatatccca gcccgccgac aaccaggagg ctgatggcga taaagccggc aaggaaggga
      901 ttttctttgg tccagttcat tggagggaga tcggtttttt cagcggaagg cgcagggtgt
      961 aaccataagc ccaggactgg ccatccggct gggtgcgctg gagcacgtat ttggccttgt
     1021 tttccggatc gatctcaaac agtccggacg ccgtgagatt ctcgacaaag gtgtcaatga
     1081 tgaccgcctg ctgggcattt tccggtatgt cgaggtaaag cccctgtaat tcgatcgcgt
     1141 cgatggtggc gggtttgggc ggaggcgttc ccggtttggc gggtgcgacc ggcccggatg
     1201 aagcgccgct gccgagcatt tttcccggtt cgatggcgac cggcccattt tgggacaatg
     1261 gggtaagctg cgtgatccag atgaatttgg gaggcaggta ggctcccaac tcatcgagca
     1321 aggtgttcca gatcacccgt tcatcaatgg ccagcaaaag cggagccgcg gctttttcca
     1381 gcttcccgcg ctccgccagg gcggcctcgg tctgggcggc caattgctta agccggttgg
     1441 tgtcgctttg aaccttctgc acgacgtccg cgagaatttc gctcgcccgc tggaaataca
     1501 gcgaggtggc gatcagggcg aaggaaacgg tgacaagggc ggcgatgagg tagggcttgc
     1561 gccgggccag atcttgttgg cggatcacgc tggccggacg gagattgatc tccatggggc
     1621 aattccggag attccgcagc cccaatccca ccagctcgcc cagggtgtgc gtcttgccgg
     1681 cgacttcgct ggcgaccgct tcattggccg cggtgacatt ccggagcgga ttgagaaact
     1741 ccacgggaaa ttgcagtttc tcggaaaaga actccgccat gtatgggaag ctcacggtgc
     1801 cgccgcacag cacggcccgc acgggctggg ttccgccctg attcgcgcgg tagaagctga
     1861 ttgagcgggt aatttcggcg tgcaagcggg taagcgtgtt ccgcacaagt ttcgaaatct
     1921 tggcctccat cgcgtcatcc ggttcggcgt aagccccgcc cagccccacg aacccctttt
     1981 ccaacttcag cttttcggcg aaggtgattt cttcacccag ctctttggcg atggcggcgc
     2041 tgatggtgtt gcccccgatc ggaatgctgc ggctgaagac cttttgtcct tcgataaaaa
     2101 tcaggttggt cgtgcgcgag ccgatgtcga tgagcaggga agtgccggcc gcgtcggact
     2161 ggttaaagcg gaaggcatta taaatggcca tcggagccac atcgatgtaa cccgccttca
     2221 gcccggcggc ttcgaccgcc gcattcaagt tggtgagctg gtctgacttg atggcgacca
     2281 atacgacgtc ccactgatcg tcgcgcggct tgcccatgat ctggtaatcc cacacgactt
     2341 cgtcgatggg gaagggcaca ttctgctggg cctcaaagcc aatgaccgaa cggacatcct
     2401 ccgcgttggc gccgggaagg cggacaaagc gggtgaacac cgactgcgaa gggagcgcgt
     2461 agttgacggt ttcccccttc ttgacccgca acgcggtccg gagttcctcg accgccagcc
     2521 gcacttgttc cgaacgggtg gcatccgccg acggatccac gatcagctcg gtttgcttga
     2581 aggcgtgcag aatgagaccg ccacccgcag tggcgtgaaa ctcggcgaga ctgatggtct
     2641 gcatgccgag gttcagggcg tagtatttgg taggtgcggc catgtcagcg tccggggtgt
     2701 cctgaaactc gggtggctaa aatccgccgg gggtttgctg ggctttgagc ggttcgtaat
     2761 aatcccaggc aaccgccgcg aagggcgttt cgttcttgtt caagacatat ccctgaacgt
     2821 ttggctggaa atcattccac cagtttggcg ggaatttccc ctggctgaga taacccacca
     2881 gctgcccgcc gcgggtgacg gcgacactga cttccttcac cgccgaaccg gccgtggccg
     2941 gctgcttgcc gtcgaaaaag cgatccagcg tgcgggggct gatgtaaacc acggttttca
     3001 gaccttttcc ggcggcgacc gcggtcaggg tgctgcggcc ggtcaacagc gtggctttgg
     3061 ccaaacctcc ggtggaggga tcccggaatc ccggattatt gagaagaatg taataattga
     3121 ccgtcaattc atccaggaac cggggcgcgc tcggactggg ccggggctgc cagttaaacg
     3181 tggtttcaat ttccagccat tcccggttct tggttttttt gcccttggga ccgaactgga
     3241 agctgaattc cggagtcgtg acataatcca cggtgacgcc ggtgacttcc accaagtcgc
     3301 cttgggcgag ctgggcggaa gcgggaagca aactgagggt ggttaaaaca acgactggga
     3361 ggagggggtt catca
//
LOCUS       DATDAY010000270         2455 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_208475, whole genome shotgun sequence.
ACCESSION   DATDAY010000270 DATDAY010000000
VERSION     DATDAY010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2455)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2455)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2455
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_208475"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(982..1845)
                     /locus_tag="VIS74_05385"
     CDS             complement(982..1845)
                     /locus_tag="VIS74_05385"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HEY5792710.1"
                     /translation="MKLSRNFLITGSVVVLAALAALILYGRYLDRPWTRDGQVQANIV
                     GIAPRVAGPIIQIPIKDNQAVKKDDLLFEIDPSTFQAAVDQAAAQLKQTQAAALKSQQ
                     NLERTNQLFVTKVVDSETLQNAQDDYTGAVADMEAAQAALETAQLNLQYTKVYSPVDG
                     YLTNMNTSLGTYVNAGEQLLALVDSSSFWVAAYFKETQLKHLAPGGKVKITLMGHAWE
                     PFEGVVNSVGWGVFLENGSAVGNGLLPQVYQTIDWVRLPQRFAVRVDVAGKPPVPLRI
                     GQTASIAVMKQ"
     gene            complement(1853..2077)
                     /locus_tag="VIS74_05390"
     CDS             complement(1853..2077)
                     /locus_tag="VIS74_05390"
                     /inference="COORDINATES: protein motif:HMM:NF019484.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HEY5792711.1"
                     /translation="MWEFIGRLKTAELQLGDFLIPWAMMIFVLGFLAAWGVVAVMERK
                     GWTRHVWHLPLFFMALAVFFGSLFGLALAP"
BASE COUNT          460 a          831 c          708 g          456 t
ORIGIN      
        1 gtgacggtga tttccgggcc gggcacgctc tcgccggcgt cgttggcggc ggtgagtcgg
       61 agggcgagcg cctcgccggc gggcagaccg gtgagaacgg cttcactttc cgaaaccgtg
      121 gccaccgggg aaaaatcagg atcgacgggg gccgcggatg gacgcggata gggatgcgga
      181 aatccgcgcc catccgtggt taaacaatat ttccgcagga aacagctttt cggcctccgt
      241 ttacttcatg gccttcatgg taaaaaaggg ccgcagtttt taacacaatc ttaatacctt
      301 tgccgccccg cttaactcac gctctttcgg cattcgcgcc atagtggctt catgcaagtc
      361 accgatttcc tgcaaatcac tctcttcctc gccgtcctca ttttgctggc gccgccgctg
      421 ggcgcgttca tggccgcggt ctttgaggga aaaccgcacg tttcttcatc ccgttctggg
      481 cgaggtggaa accgggcttt accgcctcat cggcgcggac ccgcggcggg aaatgtccgg
      541 gcggacttac gcgctcgccc tgctggcctt caacctggcg ggatttctcg tcctgttcgc
      601 cctgctgctt tgccaaggct cgctgccgcc aagcccttcc acacgcagga actgctggcc
      661 cggctgcgcg tgctcgcccg gaacgccgga aaaatcgtca cccacaagca gctgctgcaa
      721 gcggtctggg gccccaaggc caccgagcaa tcccaatacc tccgcgtttt cctcagccac
      781 atccggcgga tgccatccgc accgagaccg gcgtgggcta tcggctgctg gattagcgca
      841 ttttcgaaag tggctggtga ctggtggagg gaagatggtt ttttcgaaag acgccttcgc
      901 ccctccagtc accctcttcc agtctccagt caccagtcat cccccccgaa aacctcccga
      961 gggcaaccgg cgggcgcctg ttcattgctt catcaccgcg atggacgccg tctggccgat
     1021 gcgcagcggg acgggcggct tgcccgcgac atccacccgg accgcaaacc gctgcggcag
     1081 ccgcacccag tcgatcgtct gatacacctg cggcagcagc ccgttgccca cggcggaacc
     1141 gttttcgaga aaaacgcccc agcccacgct gttgaccacg ccctcgaacg gctcccaggc
     1201 gtggcccatg agcgtgattt tgaccttgcc gcccggcgcg aggtgcttga gctgcgtttc
     1261 cttgaaatac gccgccaccc agaacgaact cgaatccacg agcgccagca gttgctcgcc
     1321 cgcgttcacg taggtgccga ggctcgtatt catgttcgta agataaccgt ccaccggcga
     1381 atagaccttc gtgtattgca aattgagctg cgccgtctcc agcgcggcct gtgcggcctc
     1441 catgtcggcg accgcgcccg tgtaatcgtc ctgcgcgttc tgcaaggtct ccgagtccac
     1501 gaccttcgtg acgaacaatt gattcgtgcg ctccaggttc tgctggctct tgagcgccgc
     1561 ggcctgggtc tgcttgagct gggccgccgc ctgatccacc gccgcctgga aagtggacgg
     1621 atcaatttca aacagcaggt catccttttt caccgcctgg ttgtccttga tgggaatctg
     1681 gataatcggc cccgccaccc gcggcgcgat gccgacaatg ttggcctgca cctggccgtc
     1741 ccgggtccag ggccggtcaa gataacgccc gtataaaatc aatgccgcca gcgccgccag
     1801 cacgaccacg gacccggtaa tgaggaagtt tcgcgagagc ttcataaaaa tctcagggcg
     1861 ccagcgcgag cccgaacagg gagccaaaaa ataccgccag cgccataaaa aacagcggca
     1921 ggtgccagac atggcgggtc catcctttcc gctccatgac cgccaccacc ccccaggcgg
     1981 ccaggaaacc gaggacgaaa atcatcatcg cccacgggat caggaaatcg ccgagctgca
     2041 gctccgccgt tttcagtctg ccgatgaact cccacatgga tgggttggaa ttacccgttc
     2101 ccctccgccg aggcaagcga gcaaactctg ggaacacgac ctcacccgct tttggagcgg
     2161 cttcgattta ggttgacgcc aaatggaggg cgccgcgccg tcggcgcctt gaaccgaccg
     2221 tggcgccgac gcagcggcgc cctccaagtc ggctgtctgc gggtattgat cggcatcacc
     2281 cggatcacgc ggcggcaacc ggctccgcgc gcagcgacgc cagcgcgtcc gggatcgaaa
     2341 cggtctgccg ccggcggtcc agaaagaaac tcacttcgcg atagcccacc tcccgcaaca
     2401 tctggagagc ctccccgtag cgatccgcca cgcgtgaagg cgaatgcgcg tccgc
//
LOCUS       DATDAY010000271         2163 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_1477028, whole genome shotgun sequence.
ACCESSION   DATDAY010000271 DATDAY010000000
VERSION     DATDAY010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2163
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1477028"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..593
                     /locus_tag="VIS74_05395"
     CDS             <1..593
                     /locus_tag="VIS74_05395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792712.1"
                     /translation="WNESVLENYYRASQPLYTTQIFIPSMGADAAPKAFGVGKIVQPR
                     LWMAHYKGQVVPPSAGKWRFWGMADNLLCVAVNGKIVLISNLGGARIPGVPWKPPEPP
                     GQLFGKQSRLTAGDWMEMRAGEPFDLDVLIGEIPGGRFASFLLIEKQGEAYETVNGYP
                     AYPIFQLAPFDTPPPKDPLAPCPRFARDGPVWKGVQ"
     gene            837..1562
                     /locus_tag="VIS74_05400"
     CDS             837..1562
                     /locus_tag="VIS74_05400"
                     /inference="COORDINATES: protein motif:HMM:NF020897.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY5792713.1"
                     /translation="MLPAQPNQSLIDGLACLQALAAADGPAGSIDLARLLDMEPTRAN
                     RLLKTLAALGFARQNSQRKYIAGPAMHVLAAQSMKGSRLLACAMPVIGELQAEGLTVA
                     LGVLWRAHVCYLFHGKPRRQLVEGVFHYELFPALKSSIGLALLARKTNREITELLKSA
                     PPGVPRALDAETRALLAAARGNGFCHVRRGRDDTSIGVVIGDPAVAGLAFAGPFSDQD
                     APRLARVLQEAAAQIEGSLNEKP"
     gene            1576..>2163
                     /locus_tag="VIS74_05405"
     CDS             1576..>2163
                     /locus_tag="VIS74_05405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792714.1"
                     /translation="MKTPLHFLFAASLGLSAAPLRAADPVVPVPAIPGVAASSVYQLR
                     VNGQEVAVNDESRFDFHTAAFCMAGTADVEIRVPGGSGTPVIRPLRHPIAPEFKDGVL
                     SFSIRAPLKLVVEVAGQLPLALLATPLESGVPEPSAPNVLYFGPGIHEAGVIRPRTGE
                     VIYLAPGALVRGRIEAKGVKDVAVKGRGILDAGNYS"
BASE COUNT          369 a          685 c          712 g          397 t
ORIGIN      
        1 gatggaacga aagcgtgctg gaaaattatt accgcgcgtc ccagccgctt tacacgacgc
       61 agattttcat tccctcgatg ggcgcggatg ccgccccgaa ggccttcgga gtcgggaaga
      121 ttgtccagcc caggctgtgg atggcgcatt acaaggggca ggtcgttccg ccctcggccg
      181 gaaaatggcg gttctggggc atggcggaca atctcctttg cgtggcggtg aatggcaaaa
      241 tcgtgctcat cagcaatctg ggcggcgcgc ggattccggg cgttccctgg aagccgccgg
      301 agccgcccgg ccaattgttc ggcaaacaga gccggctgac cgcgggcgac tggatggaaa
      361 tgcgggccgg cgagcccttt gatttggacg tgttgatcgg ggaaattccc ggcgggcggt
      421 tcgcctcgtt cctgttgatc gagaaacaag gagaggccta cgaaaccgtg aacggttatc
      481 cggcctatcc catcttccag ctcgcgccct tcgatacgcc gccgccgaag gatcccctgg
      541 cgccttgtcc ccgcttcgcg cgggacggcc cggtctggaa gggagtgcag tgaggggggc
      601 ggggaaaggt gactggtgac tggtgatttg aaaaggggat gaaggattcg gaagcaggca
      661 tgccgcccgg ggcgcgcaat ttgggaacga acggaaaaca gaacgggcca agcttcccgc
      721 taacgcggcc tggcccgcca ttcacgattc accagtcacc gccttcacct cctcccgttg
      781 aaaatccccc aaaaaacagg agttgctttc gccaataata ttgctattat agcaacatgc
      841 ttcccgccca gccgaatcaa agcctcatcg acggcctggc ctgcctccag gcgctcgcgg
      901 cggcggatgg tcccgccggt tcgatcgatc tggcgcgcct tctggacatg gagccgacgc
      961 gcgccaaccg cctgctcaag acgctggcgg cgctggggtt cgcccggcag aattcgcagc
     1021 gaaaatacat cgccggcccg gcgatgcacg tgctggcggc gcagagcatg aagggctcgc
     1081 gcctgctggc ctgcgccatg ccggtcatcg gcgagttgca ggcggagggg ctgacggtcg
     1141 cgctcggcgt gttgtggcgg gcgcacgtgt gttacctttt tcacggcaag ccgcggcggc
     1201 aactcgtcga gggcgttttt cattacgagc tttttccggc gctcaaatcg agcatcggcc
     1261 tcgcgctgct ggcgcggaaa acgaaccgcg agatcacgga actcctgaag agcgcgcccc
     1321 ccggcgttcc ccgcgcgctg gacgcggaga cgcgcgcgct gctggccgcg gcgcgcggga
     1381 atggcttctg ccatgtgcgg cgcgggcgtg acgataccag catcggcgtc gtcatcggcg
     1441 atcccgcggt cgccggattg gcttttgcgg gccccttttc cgaccaggat gcgccccgcc
     1501 tggcgcgggt tctccaggag gccgccgcgc aaatcgaagg ttccttgaat gaaaagccgt
     1561 agccgccaag ctctcatgaa aacccccctt catttccttt tcgccgcctc cctggggctc
     1621 tccgccgccc cgctgcgcgc cgccgatccg gtcgttcccg tgccggcgat tcccggcgtg
     1681 gccgcgagct cggtgtatca gctccgggtc aacggccagg aggtcgccgt caacgacgaa
     1741 agccgcttcg attttcacac ggccgcattt tgcatggcgg gaacggcgga cgtggaaatc
     1801 cgggttccgg gcggatcggg cactccggtc atccggcctt tgcgtcaccc gatcgcgccg
     1861 gagtttaagg atggcgtcct gagcttttcc atccgcgcgc cgctcaagct cgtggtggaa
     1921 gtggcggggc agcttccgct cgcgctcctg gccactccgc ttgaaagcgg ggtgcccgaa
     1981 ccctccgcgc ccaatgtcct ttattttgga ccgggcattc acgaagccgg agtgatccgg
     2041 ccccggacgg gcgaagtgat ctatctcgct cccggcgccc tggtgcgggg aaggatcgaa
     2101 gcaaagggcg taaaagacgt tgccgtgaaa ggccggggca ttttggacgc gggcaactat
     2161 tcc
//
LOCUS       DATDAY010000272         7293 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_1297158, whole genome shotgun sequence.
ACCESSION   DATDAY010000272 DATDAY010000000
VERSION     DATDAY010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 7293)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7293)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7293
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1297158"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..675)
                     /locus_tag="VIS74_05410"
     CDS             complement(<1..675)
                     /locus_tag="VIS74_05410"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEY5792715.1"
                     /translation="MSILFIRGSGYFQGRISEILDFFIGSPRKKTGIVLTVRPLFRKG
                     KEDIYPMKPRKLSAFTLIELLVVISIIAILASLAIPAVTGALTRGQMTQTLNNARQMT
                     LATQQMSLDSFTAGSGPAWTTQNGTDFSTLQEFIDNLVNNNYLTANDIKKLLAGPGVT
                     AVTVSTTNATVAPASSAFSFFQTSESTPNERAFLVTKNWASSALTTNAPYGNKGFVVF
                     RKGGDGS"
     gene            986..1309
                     /locus_tag="VIS74_05415"
     CDS             986..1309
                     /locus_tag="VIS74_05415"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HEY5792716.1"
                     /translation="MSPVHENFISRTVENLRKGLDPEAIYLFGSYARGEETPDSDVDF
                     LVIVPHSDLPRYRRSQIAREIAADARLPKDILVLTREEWQRGLRVPVSLPSTVRREGR
                     LLYGN"
     gene            1299..1703
                     /locus_tag="VIS74_05420"
     CDS             1299..1703
                     /locus_tag="VIS74_05420"
                     /inference="COORDINATES: protein motif:HMM:NF017020.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HEY5792717.1"
                     /translation="MGTEKSREIEAWLLKAQNDLDAARLLAEADPPKRDIAVYHCQQA
                     AEKTLKAWLVFRDIPFPKTHDLEKLLSLDLPNTPALARLAGHGHILSPYAVEFRYPGD
                     LIEPDGKTCEEALRLASEVGVLIRALLKGNAV"
     gene            complement(1907..4120)
                     /gene="glgB"
                     /locus_tag="VIS74_05425"
     CDS             complement(1907..4120)
                     /gene="glgB"
                     /locus_tag="VIS74_05425"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HEY5792718.1"
                     /translation="MNPSVLPSDDFYPLIEARHCDPFKILGMRDIGGRLGARVFRPDA
                     RAATVVSLTGDKRRFPLKEVHPGLFESAFGDADTPFPYELELTSHEGVAWTERDVYSF
                     GPVLGEMDIYLFSEGSHYEVYRKLGAHLIEKDGVRGTHFAVWAPNSQRVSVVGDWNHW
                     DGRVHTMRKMIPAGIWEIFIPGVGEGAHYKFEVRGPHGDIQLKTDPFAFFAQHGTQTG
                     CMVYDLERYGWGDAEWMQKRSEGNSYEKPMSIYEVHLGSWQRVAEEGNRPLSYLELCD
                     RLIPYVKEMGFTHIELMPVMEHPFDGSWGYQVVNYYAPTSRHGTPDEFRHFVDECHQA
                     GIGVILDWVPGHFPKDAHGLARYDGTCLYEHEDPRLGEHQDWGTLIFNYGRNEVRNFL
                     LGNALFWLDQYHVDGLRVDAVASMLYLDYSRQPGEWLPNCFGGRENLDAISFLKRFNE
                     VCYERHPGVATIAEESTAWPGVSRPTYLGGLGFGYKWNMGWMNDSLRYIAREPIHRRF
                     HQGDITFSMLYAFHEHFVLVLSHDEVVHGKGSLLNKMPGDDWQKFANVRLFLAWMWAH
                     PGKKLIFQGMEFGQWNEWNHGQSLDWHLTAFHSHGGISRLVQHLNWLYTHEPAFFELD
                     ESYEGYEWIDFHDSDNSVWSFMRKARNGDTLVFAVNATPVSRGGYRIGVNAPGFYHEL
                     LNTDAEIYGGGNVGNYGGMPAEPVAWQGKNHSIQIELPPLAVVAFKWQASPAGQG"
     gene            complement(4268..5398)
                     /locus_tag="VIS74_05430"
     CDS             complement(4268..5398)
                     /locus_tag="VIS74_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HEY5792719.1"
                     /translation="MSVIAKGLEGIVANSTRLSDVRGLEGVLLYAGYDINELAGKTSY
                     EEIIHLLFYGELPTSAQLADLKKRLAAQRELPADLAGFLRTAPPEASPMDVIRTAVSM
                     LGLYDPVRASNPENLEPDRERAFSLIAKIGVIAAYFHRARQGKDLPPVRTDLSEAAHF
                     LYLLNGEAPGIDAEKTLDVAYVLHADHGMNASTFTARVVIATLSDIYSAITGAIGSLK
                     GPLHGGANEGVIHMLQEIGSEDKVDAWVDEQLAQKKKIMGIGHRVYRTLDPRAPHLRT
                     MAIKLTEQLGEAKWIRMSERIATLMRERKKLNANVDFYSATVYYSLGIPTDMFTPIFA
                     IARTSGWCAHVLEQLEDNRLYRPLSEYTGPEYGKKFVPIENR"
     gene            complement(5508..6440)
                     /locus_tag="VIS74_05435"
     CDS             complement(5508..6440)
                     /locus_tag="VIS74_05435"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEY5792720.1"
                     /translation="MKKRNLNDWAVVGMVILCSLVVLAALSMALMGKTFGRPERVVRV
                     AFPDITGVKESSAVRYAGAVAGFVYRVRPLTSEQRIASGHPENTVEIELALSRSVPAL
                     TQGARAEISADTLLSEKFVQLVPGPADSPVLAAGAILTGQAPTPFDQLAHTADRTLFA
                     ISQLLSGDKLDTGTIFSDLRSVLSQVEALLKQAEPVLANAGSLVNQGNTLLADPRINE
                     TLTQLRTASASLARLSQSGEGVLQRNQANLDSSLQSLKVSLQNLKLLTTYGAIFTRGV
                     AERPQSVVWGNRRPPEIPAQADILKAARTAVPPD"
     gene            complement(6493..>7293)
                     /locus_tag="VIS74_05440"
     CDS             complement(6493..>7293)
                     /locus_tag="VIS74_05440"
                     /inference="COORDINATES: protein motif:HMM:NF020071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase C-terminal
                     domain-containing protein"
                     /protein_id="HEY5792721.1"
                     /translation="QDSRLLVAAEITEIGKGVGDAQVTLSLATEIEEDWLREMFPADF
                     VDGNEIIFDPSQNRVVQRRERRFRGLVLESSDRDVSPDDEVALRLAEGIEKHELPLPG
                     WDDAATQWIIRLNSLAKWMPELGLPAFGDEPRRDILLNLCEDAVSYRDVKDRSARPFL
                     SAWLRPGRQALLDRHAPERVELPNGRKAKLVYREGAGPTLSAKIQELYGMKDTPKVAG
                     GRIPVTLEILAPSHRPVQTTQDLASFWSNAYPKLKAELQRRYPKHEWR"
BASE COUNT         1384 a         2151 c         2276 g         1482 t
ORIGIN      
        1 cgagccgtcg ccgccttttc ggaacaccac aaaccctttg tttccgtaag gggcgttcgt
       61 ggttaacgca ctgcttgccc agtttttggt caccagaaaa gcgcgttcat tgggagtgct
      121 ttccgaggtt tgaaaaaaag aaaaagcgga tgaagctggg gccacggttg cattggtggt
      181 gctgaccgta acggccgtca ctccaggacc cgccaacaat tttttgatat cattagcggt
      241 caaataattg ttattgacta ggttatcgat aaattcttgg agagtagaga agtctgtgcc
      301 gttttgagtt gtccacgctg gaccacttcc cgccgtgaag ctgtccagcg acatctgttg
      361 cgtcgccaaa gtcatttggc gagcgttgtt taatgtctgg gtcatctggc cgcgggtgag
      421 cgctccagtg acggccggaa tcgccagcga ggccaggatt gcaatgatgg aaataaccac
      481 caaaagttca atgagggtga aggcagatag tttgcggggt ttcatggggt aaatatcttc
      541 ttttcctttc cgaaataaag ggcgcaccgt caacacaatt cccgtctttt ttcgaggtga
      601 tcctataaaa aagtccaaaa tttcggaaat tcttccctga aaataccctg aaccacggat
      661 gaacaggatc gacacggatg gctctgaaaa ggatgtgaat gcgacgtggt ctttttttgc
      721 tataatccgt gtgaatcggt gttcatccgt ggttaaagat tgaaccaagg atggacaccg
      781 atggaggaat gcggatgagg tgaagatctt tcgcaaaggt tttgggtagg gacggctcaa
      841 tgagccgtcc gtggaatcgg ggttttatcc gcagattacc ggggttttgc tcttcagctc
      901 atggtaaaaa gacaccttgc gcgctcagcg ggaagttccg gcaaaatcga agtgacttcg
      961 ccgtccggcg cgatatgctg ccggcgtgtc tccggttcac gaaaacttca tttcaagaac
     1021 ggtggaaaac ctgcgcaagg ggcttgatcc cgaagcgatc tacctcttcg gctcctatgc
     1081 ccgcggagag gaaactccgg atagcgacgt ggatttcctg gtgatcgtcc cgcactcgga
     1141 cctgccgcgc taccggcgca gccaaatcgc gcgcgagatc gccgcggacg cgcggttgcc
     1201 caaggacatt ctcgttttga cccgcgagga gtggcagcgt ggactccgcg tcccggtctc
     1261 gctgcccagc accgtccggc gcgagggcag gctcctttat gggaactgaa aaatcccggg
     1321 aaatcgaggc atggctgctc aaagcgcaaa atgacctcga tgccgcccgg ctcctggcgg
     1381 aggccgatcc gcccaaacgg gatattgctg tctatcattg ccagcaagcc gccgagaaaa
     1441 ctctgaaggc atggcttgtc tttcgcgaca ttccctttcc caaaacgcac gacctggaga
     1501 aacttctttc cctcgacctc ccgaacactc cggcgttggc gcgcctggct ggccacggac
     1561 acattctctc tccttacgcc gtcgagttcc gctatccggg agatctgatc gagccggatg
     1621 ggaaaacttg cgaggaagca ttgcgcctgg cttccgaagt cggagtgctc atccgagctt
     1681 tgcttaaagg aaatgcggtc tgacggcggt ggatttgatg ggcgggattt ttaaccgcgg
     1741 tggtaagtcg aaccacggat ggacacggat taacacgaat ggaggaatgc gaatctcccc
     1801 cgaagatttt tgggtaggga cggctcggcg agccgtccct acctttgttt tactataatc
     1861 cgtgtggatc cgtgcctgtt catccgtggt tgaatgtcga accggactac ccctgacccg
     1921 ccggagaggc ttgccacttg aacgccacca cggccagcgg cgggagttcg atctggatcg
     1981 aatggttttt cccctgccac gccacgggtt ccgccggcat gccgccatag ttgccgacat
     2041 tgccgccgcc gtagatttcg gcgtccgtgt tcaggagctc gtgataaaac ccgggtgcat
     2101 tgacgccgat gcggtagccg ccgcgcgaga ccggcgtggc gttgaccgcg aagacgagcg
     2161 tgtcgccatt gcgcgccttg cgcatgaagg accagaccga attgtcgctg tcgtgaaagt
     2221 cgatccactc gtaaccctcg tagctttcgt ccagctcgaa gaacgccggt tcgtgggtgt
     2281 agagccagtt gagatgctgg accagccggc tgattccgcc atgggagtgg aaggcggtca
     2341 ggtgccagtc gaggctctga ccgtgattcc attcgttcca ctggccgaat tccattccct
     2401 ggaagatcag cttcttgccg gggtgcgccc acatccaggc gaggaaaagc cggacattgg
     2461 cgaatttctg ccagtcgtcg ccgggcatct tgttcagaag cgaacccttc ccgtggacga
     2521 cctcgtcgtg gctgagcacg aggacaaaat gctcgtggaa ggcgtagagc atggagaagg
     2581 tgatgtcgcc ctggtgaaag cggcggtgga tgggctcgcg ggcgatgtaa cgcagcgaat
     2641 cattcatcca gcccatgttc cacttgtagc cgaagcccag gccgccgagg taggtgggcc
     2701 gggagacgcc gggccacgcg gtggattcct ccgcgatcgt ggccacgccg gggtgccgct
     2761 cgtagcagac ctcgttgaag cgcttgagga aggagatcgc gtcgaggttt tcgcgcccgc
     2821 cgaagcagtt gggcaaccat tccccgggct ggcgcgagta gtcgagatac agcatggagg
     2881 ccaccgcgtc cacgcgcagg ccgtccacgt gatactggtc cagccagaac aaggcgttgc
     2941 cgagcaggaa attccggacc tcgttgcggc catagttgaa gatcagcgtg ccccagtcct
     3001 gatgctcgcc cagccgcggg tcctcgtgct cgtagaggca ggtgccgtcg tagcgcgcga
     3061 gcccgtgcgc gtccttggga aaatggccgg gcacccagtc gaggatgacg ccgatgccgg
     3121 cctggtggca ttcatccacg aagtgacgga attcatccgg cgtgccgtgg cggctggtcg
     3181 gggcgtaata gttgacgacc tgataccccc acgagccgtc gaacgggtgc tccatcaccg
     3241 gcatcagctc gatgtgggtg aagcccattt ccttcacgta gggaatgagc cggtcgcaga
     3301 gctccagata actcaggggc cggttgcctt cctccgccac ccgctgccac gaaccgaggt
     3361 gcacctcgta gatggacatc ggcttttcgt aggaattgcc ctcgctccgt ttttgcatcc
     3421 actcggcgtc gccccatccg taacgctcca agtcgtaaac catgcagccc gtctgggtgc
     3481 cgtgctgggc gaagaaggcg aagggatcgg tcttgagctg gatgtcgcca tgggggccgc
     3541 gcacctcaaa cttgtagtgg gcgccttccc cgacgccggg aatgaagatc tcccagatgc
     3601 cggcggggat cattttccgc atggtatgca cgcgcccgtc ccaatgattc cagtcgccca
     3661 ccacgctgac ccgctgggag ttgggcgccc acaccgcgaa atgcgtgccg cggacgccgt
     3721 ccttttcgat caggtgcgcg cccagcttgc ggtaaacctc gtaatggctg ccttcgctga
     3781 agaggtaaat atccatctcg cccaatacgg gcccaaatga gtaaacgtcc cgttcggtcc
     3841 acgccacgcc ctcgtggctg gtcagttcca gctcgtaggg gaagggggtg tccgcatcgc
     3901 cgaaggcgga ttcgaacaac ccgggatgca cttccttcag cggaaatcgc cgcttgtccc
     3961 ccgtcagcga gacgacggtg gccgcccggg cgtccggacg gaacacgcgc gccccgagcc
     4021 ggccgccgat gtcccgcatg ccgagaatct tgaagggatc gcaatgccgg gcttcgatga
     4081 ggggataaaa gtcgtcgctg gggagaacac taggattcat gggtaaggga cattctattc
     4141 ctaatttccc gccactggca agcggtcgcg caggtatggg aattgtagcc gggatcggtg
     4201 atcccggctg gcttgaattt gattttcgaa tcggaaacgg ccgggatcac cgatcccggc
     4261 tacaccgtta ccggttttcg atcggcacga attttttgcc gtattccggg ccggtgtatt
     4321 cgctcagcgg gcggtagagc cggttgtcct cgagctgttc gagcacatgc gcgcaccaac
     4381 ccgaggtgcg ggcgatcgcg aagatcgggg tgaacatgtc ggtcggaatg ccgagcgaat
     4441 aataaaccgt cgcggagtag aaatccacgt tggcgttgag cttcttgcgc tcgcgcatga
     4501 gcgtggcgat gcgttccgac atccggatcc atttggcttc gccgagctgt tcggtgagct
     4561 tgatggccat cgtccgcagg tgcggggcgc gcgggtcgag cgtgcgatag acccggtggc
     4621 cgatgcccat gatctttttc ttctgcgcga gctgttcgtc cacccacgcg tccaccttgt
     4681 cctcggaacc gatttcctga agcatgtgaa tcacgccctc gttggcgccg ccgtgcagcg
     4741 ggcccttgag cgagccgatg gcgccggtga tggcggaata aatgtccgaa agcgtggcga
     4801 tgaccacgcg cgccgtgaag gtgctggcgt tcatgccgtg gtcggcgtgc agcacgtagg
     4861 cgacatccag cgtcttttcc gcgtcgatgc cgggagcctc cccgttgagc agataaagga
     4921 aatgcgcggc ttcgctcagg tccgtgcgca ccggcggcag atccttgccc tggcgggcgc
     4981 gatggaaata ggcggcgatg acgccgatct tggcaatcag gctgaaggcg cgttcgcggt
     5041 ccggctcgag attttccgga ttgctcgcgc ggaccggatc atacaggccg agcatcgaaa
     5101 ccgcggtgcg aatcacgtcc atggggctgg cctccggcgg ggcggtccgc aaaaagcccg
     5161 ccaaatccgc cggcagctcg cgctgggcgg cgaggcgctt tttcagatcg gccaattgcg
     5221 cgctggtcgg cagctcgcca taaaacagaa ggtggatgat ttcctcatag ctcgtctttc
     5281 cggcgagttc gttgatgtcg taaccggcgt agagaagcac cccttccagg cccctgacat
     5341 cactcagacg agtgctgttt gcgacgattc cttcgagtcc tttggcaata actgacatat
     5401 gtttttggtg atctaatgat tcaggtttcg gtaacagccg cgagccgcct ctcggctgca
     5461 ttttggaaaa tacatgaact cagggaaagc ggaagcattt ctctttttta gtccggagga
     5521 accgcggtgc gggccgcttt cagaatgtcc gcctgcgcgg gaatttccgg cggccggcgg
     5581 tttccccaga cgacgctttg cgggcgctcg gccaccccgc gggtaaagat ggcgccatac
     5641 gtggtgagca gcttgagatt ctgcaggctg accttcaggc tttgcagcga actgtcgaga
     5701 ttggcctggt tgcgctgcag gacgccttcg ccggactggg acaggcgggc cagcgacgcg
     5761 gaagcggtgc ggagctgggt cagggtttca ttgatgcgcg gatcggccag cagcgtgttg
     5821 ccctgattca ccagcgagcc cgcgttcgcc aggaccggct ccgcctgctt gagcaatgcc
     5881 tccacctggc tgagcaccga ccggagatcc gagaaaatcg tgcccgtgtc gagcttgtcg
     5941 ccgctcagca gctgggaaat cgcgaacaag gtgcggtccg ccgtgtgcgc cagctgatcg
     6001 aagggcgtgg gcgcctggcc ggtgagaatg gcgccggcgg cgaggaccgg ggaatccgcc
     6061 gggccgggaa ccagctggac aaatttttcg ctcaaaagcg tgtcggcgga aatctccgcg
     6121 cgcgccccct gcgtcagcgc cggaacggaa cggctcaggg ccagttcgat ttccaccgtg
     6181 ttttccggat ggccggaggc gatccgctgt tccgaggtca acgggcgcac gcgatacaca
     6241 aatcccgcca ccgcgccggc gtaacgcacc gccgagcttt ccttcacgcc ggtgatgtcc
     6301 ggaaacgcca cgcgcacgac ccgctcgggg cggccaaagg ttttgcccat gagcgccatg
     6361 ctcagcgcgg cgagaacgac gaggctgcac aggatcacca ttcccaccac cgcccagtcg
     6421 ttgaggtttc gtttcttcat gcgcgccagc gcaaaaacaa agagactttt cggattccgg
     6481 gcgagcgaaa aatcaacgcc actcgtgttt cggataccgc cgctgcaatt ccgccttcaa
     6541 tttcggatac gcgttgctcc agaaactcgc cagatcctgc gtggtctgca ccgggcggtg
     6601 gctcggggcg aggatttcca gggtgaccgg aatccgcccg cccgcgactt tgggcgtgtc
     6661 cttcattcca tagagctcct ggattttcgc gctcagggtc ggccccgcgc cttcgcgata
     6721 gaccagcttc gccttgcggc cgttcggcaa ctccacccgc tcgggcgcgt gccggtcgag
     6781 caaggcctgc cggccgggcc gcagccatgc gctgaggaac ggccgggcgg agcggtcctt
     6841 cacgtcgcgg taactcaccg cgtcctcgca gagattgagc agaatgtcgc gccgcggctc
     6901 gtcgccgaag gccggcagtc cgagttcggg catccatttt gccagcgaat tgagccggat
     6961 gatccattgc gtggccgcat cgtcccagcc ggggagcggc agttcgtgtt tttcgattcc
     7021 ctcggccagc cgcagggcga cctcgtcgtc gggcgagacg tcgcgatccg aggattccag
     7081 caccagcccg cggaaacggc gttcgcgccg ctggaccacg cggttctggg acggatcgaa
     7141 aataatctca ttcccgtcca cgaaatccgc ggggaacatt tcccgcagcc aatcctcctc
     7201 gatctccgtg gcgaggctga gcgtgacctg ggcgtcgccc acgcccttgc cgatctcggt
     7261 gatttcggcg gcgacgagca gccggctgtc ctg
//
LOCUS       DATDAY010000273         2602 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_1280785, whole genome shotgun sequence.
ACCESSION   DATDAY010000273 DATDAY010000000
VERSION     DATDAY010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2602)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2602)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2602
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1280785"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            284..2002
                     /locus_tag="VIS74_05445"
     CDS             284..2002
                     /locus_tag="VIS74_05445"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEY5792722.1"
                     /translation="MALIQGDQLPNYLIGTSGDDTISAFAGNDTIVPGLGEDSIDGGS
                     GLDKLILDWSQENSLSGGAFLAQADLSYYNSVDIGGSGVINKLGPEVSELLNRGFRDA
                     KRGFYTSNGASGKHSEVRNVELFEVTGTAGNDSFFGTRRADTFRGGAGNDLFDAGIGF
                     SYYHNLDGAGAADVIEGGMGIDTLVALDLRHETQSFVVKDDGSFVYSNARGMKISGIE
                     AFETLALGRGNDSVEYTGYSRNMIFAGAGNDTINPGQGSADYVVGGKGSDLLVLDWTT
                     GDLELTLRADENFARSFAYVNYLHFYEIERFQVTGSKWKDYLDAGSGTDTLSGERGDD
                     SLSGHAGADLLIGGSGNDWLDGGTGADTLVGGTGNDTYVVNSSRDTIQEFNGEGRDVV
                     QSSVSFSLANLFVENLTLTGFATHGIGNLRANILSGNEVANLLEGKSGNDSLTGAGGN
                     DTLAGYNRSTRGIDESDTLTGGLGIDFFMLGDASGRFYDDGKTSSAGTKDYALITDFT
                     VGSDKLQLKGAASDYHLGASPVAGVDGSALFYENGATDELIAIVKSANSKVLTVANTI
                     NTAVFA"
     gene            complement(2218..>2602)
                     /locus_tag="VIS74_05450"
     CDS             complement(2218..>2602)
                     /locus_tag="VIS74_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006983834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEY5792723.1"
                     /translation="ALTPAEVAAIKEKYFLEILDEAKPIGQVVEFARSRYGKAPLAVA
                     SGGHRAIVIAALEVLGIRELFDTVIGAEDYAHGKPAPDPFLLAAKRLGVRPEECLVFE
                     DTENGAKAARAAGMDYVLVPDPRRS"
BASE COUNT          574 a          691 c          770 g          567 t
ORIGIN      
        1 gaggaacggg aatatcagga aatcgccgcg gaactgcgct cccggatttc cggacgggaa
       61 tttgaagata actggcaggc gggccggaca atgccggtgc atgaattgct ggcggcggga
      121 ttaacggcgg cctgagcgcg gattgggcgt ttctgatcct ttcggaaacc acagatgcac
      181 acttattcac acggataatt gcccccccac ttcttatgat ttgacgattg acttggaaga
      241 actcgaagac atacggtggt tcctaattta actcgttcca agtatggccc tcatccaagg
      301 cgatcaactg ccgaattatt taatcggaac gagcggtgat gacacgatct ccgcctttgc
      361 cggcaacgac acgattgtcc cgggtctggg cgaggactcc atcgatggcg gctcggggct
      421 ggataaatta atacttgatt ggtcgcagga gaattcactg agtggcgggg catttttagc
      481 tcaagccgac cttagctact acaacagcgt ggatattggt ggctcaggcg tcattaacaa
      541 gcttggaccg gaagttagtg agttgctgaa ccgaggattt cgggatgcca agcggggctt
      601 ttatacctcg aatggcgcca gtggaaagca ttccgaagtt cggaacgtgg aacttttcga
      661 ggtaaccggg acggcgggca acgattcctt ctttggcacc cgaagagcgg atacctttcg
      721 gggcggagcg ggaaatgatc ttttcgatgc cggaataggc ttttcttatt atcacaattt
      781 ggatggcgct ggcgccgccg acgtcatcga aggaggcatg ggcatcgata ccttggtagc
      841 attggatctc cggcacgaaa cccaaagctt tgtggtgaag gacgacggtt cgtttgtgta
      901 ttccaatgcc cggggaatga agatttccgg cattgaagca tttgaaaccc ttgcgctcgg
      961 tcgcggcaac gactcggtcg aatacacagg ttactccaga aatatgattt ttgcgggagc
     1021 cgggaatgat accattaatc ccggacaggg aagcgccgac tatgtcgtgg gaggcaaagg
     1081 ttccgatctt ctagttctgg actggactac tggggattta gagctgacgt tacgggcgga
     1141 cgaaaatttt gcgcgttctt ttgcttatgt aaattatctg catttctatg agatcgaacg
     1201 atttcaggta accggaagca aatggaagga ttatctggac gccggatccg ggacagacac
     1261 cctgtccggg gagagaggcg atgacagtct gtctggccac gccggggcgg atttgctcat
     1321 cggaggttcg ggaaacgatt ggttggacgg cggcaccgga gccgacaccc tcgtgggtgg
     1381 aaccggaaat gacacctacg tggtaaatag ctctcgtgac acgatccaag aattcaatgg
     1441 agaaggcaga gatgtcgtgc agagcagcgt gagcttctcc ctggccaacc tttttgttga
     1501 aaatctcacc ctcaccggct ttgcgaccca tggcatcggc aatcttcggg ccaatatctt
     1561 gtccggaaat gaggtggcga atcttctgga aggcaagtct ggcaacgaca gcctgaccgg
     1621 tgctggggga aatgacaccc tggcgggcta taatcgatcc actcgcggga tcgatgagtc
     1681 cgataccctt acgggcgggt tgggcattga tttcttcatg ctgggtgatg ccagcggccg
     1741 cttttacgac gatggcaaga cgagcagtgc cggcacgaaa gactacgcgc tgatcaccga
     1801 ctttaccgtg ggctcggaca agctccagct caagggtgcg gccagcgatt atcatctcgg
     1861 cgcttcgccg gtggccggag tggatggcag tgcgctcttc tacgaaaatg gggccaccga
     1921 tgaactgatc gccatcgtca agagcgcgaa ctcaaaagtc ctgacggtag cgaatacgat
     1981 caatactgcc gtctttgcat aacggataat aaccccggag agaatgtcca aaaggtcaac
     2041 tcacgcaaag gcgcgaagtc gcaaagatat ccagccaaag acactttttt cggcttcgcg
     2101 tctttgcgtg aggctcaatc ctctttattt cttcttcggc gtttttcggt ggtaaatcac
     2161 ggacggttcg gcgagccgtc cctcgggggc ggcttcgccg aagccgccgc agctgcgcta
     2221 actccgccgc ggatcgggca ccagcacgta atccatgccc gcggcccggg cggccttggc
     2281 gccgttttcg gtgtcctcaa acacgaggca ttcttccggg cgcaccccga ggcgcttcgc
     2341 cgccagcagg aaaggatccg gcgccggctt gccgtgcgcg tagtcctcgg cgccgatgac
     2401 cgtgtcgaac agctcccgaa tccccagcac ctccaaagcc gcgatcacga tcgcccgatg
     2461 cccgccggag gccaccgcca gcggggcctt gccgtatcgg gaacgcgcga attccaccac
     2521 ctggccgatc ggtttcgcct catccaggat ttcgagaaaa tacttttcct tgatggccgc
     2581 cacctccgcc ggcgtgagcg cg
//
LOCUS       DATDAY010000274         5240 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_1283846, whole genome shotgun sequence.
ACCESSION   DATDAY010000274 DATDAY010000000
VERSION     DATDAY010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 5240)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5240)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5240
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1283846"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(192..2039)
                     /gene="glmS"
                     /locus_tag="VIS74_05455"
     CDS             complement(192..2039)
                     /gene="glmS"
                     /locus_tag="VIS74_05455"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEY5792724.1"
                     /translation="MCGIVAYVGKAQATPILLDGLRRLEYRGYDSSGVALLAGGDIHV
                     RKTVGRIANLADLIGREPVAGSAGISHTRWATHGGVTDANAHPHLDQSGKLALVHNGV
                     IENYQQLREELQAEGHVFKSQTDTEVLAHLIGRAFERRKETTRGSLVEALRDALHHVI
                     GTYGIAVVHAGLPDFVVGARRGSPLVLGIGRDQHFLASDVSAIVGHTRDVVYLNDYEI
                     VAIQGAEFEITTLSGSGSDYQISKVEFTAEDVSKGDYPHYMLKEMLEQPQSVFDAMRG
                     RLSPEEATAKLGGLNMTNAELRGVERIVLSACGTAFHAALIGEYLIESLAHLPVECEF
                     ASEFRYRNLPMTRDTLVFVVSQSGETADTLAALRESRRKGHRTLGICNNVGSTIARES
                     DGGVYMHAGPEIGVAATKSFTSQVTILTLIALLLGRIRHLSTSEGLRILAELEALPKK
                     IEEALKLDAQVKAIAQKYAQSPNFLFFGRQYNYPVALEGALKLKEITYIPAMGHPSAE
                     LKHGVIALINEATPSVFIAPDDAVFEKNLSNIEEVKARKGPVIAIGTEGNTALAKVAD
                     DVILIPKAPDYLTPILAVIPLQLFAYHIAVELGCDVDKPRNLAKSVTVE"
     gene            2098..3390
                     /locus_tag="VIS74_05460"
     CDS             2098..3390
                     /locus_tag="VIS74_05460"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HEY5792725.1"
                     /translation="MKRPLTPPFQRTLAIVMGGGVGSRLFPLTKERAKPAVPLGGKYR
                     LVDIPISNCLNSGIRSIYVLTQFNSTSLHRHINASYKFDHFSPSFVEILAAQQTPEGS
                     SWYQGTADAVRQNMRYFLEQPYDYFIILSGDQLYRMDYREILRQHAESESDITIATIP
                     VNRDAAKDFGIMDTDATKRIVKFVEKPKEDVVLDGLRIPEAVLESIGKSPDEDLYQAS
                     MGIYVFNRQVLCDCLDNDLVDFGKNIIPKAISDYKVDAFIFQGYWEDIGTIRAFFDAN
                     LNLTSDQPEYSFYEAGAPIYTHPRYLPASVVRDTQINRAIISDGCIIDCESIERCVIG
                     VRSVIQSGTKISNAIIMGADIYERDLPEPVFGQPAIGIGKDCVIQNAIIDKNARVGNQ
                     VVITSEGKEKDLDGDGFYVREGIVVVPKNSVIPDGTWI"
     gene            3403..3966
                     /locus_tag="VIS74_05465"
     CDS             3403..3966
                     /locus_tag="VIS74_05465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792726.1"
                     /translation="MRSLLLLAVVLLLPGCLFEKPITGPSRSINTWMLGVWEFPKKNG
                     ERLQAMVVPKDNDHYAIYLREFTKGRKLKSIKEYDAWISRVGLVSFLTVKVDGQYCPV
                     HFQLLSPVEIRLRLPDFDPGSEALTPYELRQQVRRKFKEGTLLTKEGEIWTKVSEIYW
                     PAEGNPGDQPANQPLRHVPQPAQMVGQ"
     gene            complement(4310..>5240)
                     /locus_tag="VIS74_05470"
     CDS             complement(4310..>5240)
                     /locus_tag="VIS74_05470"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HDIG domain-containing metalloprotein"
                     /protein_id="HEY5792727.1"
                     /translation="IWESLPSTDWQMIGWQSVAALGVGIGTAIVVTGLLPLLETIFGV
                     TTDISWLEMADLNHPLLKRLSLEAPGTYHHSLAVANLAEAAAERVGANPTICRVSAYF
                     HDIGKLVKPDYFTENMPAGENPHDELTPTMSALIIIAHVKEGVDLALKHKLNSVIVDG
                     IRQHHGTSLVGYFYQRARQQQKDAELGGKIMNIRPEDIPQVSEGNFRYPGPRPQTKET
                     AILSLADAVESASRSLERPTPQRIEDLVKSLVESRLEDHQLDEAPLTLEEIQRISKSL
                     TLTLQSMMHSRIAYPRREKDDKKDGAKKDAKVA"
BASE COUNT          960 a         1507 c         1715 g         1058 t
ORIGIN      
        1 ggcagccgga gcgaagacag cctgtcccgc ggaagcggga cagcccgagg ggcgcacgaa
       61 gtgcggcaat tgtgccaacc gcctgtgttt gcggggaaac ggggaccccg catccccgtt
      121 tacgctgagc ggttggcaca accgcccctc caacgcgttg gatattttcg gaagcttctc
      181 ttaaaaatcg gttactccac cgtcacgctc ttggcgaggt tgcgcggctt gtccacgtcg
      241 cagccgagtt cgacggcgat gtgataggca aacagctgga gcgggatgac ggcgaggatc
      301 ggcgtcaggt aatcgggcgc tttcgggatg aggatcacgt catcggcgac tttggctagc
      361 gcggtgtttc cctcggtgcc gatggcgatg accggcccct tgcgcgcctt gacctcctcg
      421 atgttgctca agtttttctc gaacacggcg tcgtccggcg cgatgaagac ggagggggtg
      481 gcctcattga tgagcgcgat gacgccgtgc ttgagctccg cgctggggtg gcccatggcg
      541 gggatgtagg tgatctcctt cagcttgagc gcgccttcga gggcgacggg gtaattgtat
      601 tgccggccga agaacaggaa gttcggcgac tgggcgtatt tttgggcgat ggccttgacc
      661 tgcgcgtcga gcttgagggc ttcctcgatt tttttcggga gcgcctcgag ttcggcgagg
      721 atgcggaggc cttcgctggt ggagaggtgc cggatgcggc cgagcaggag ggcgatcaag
      781 gtcaggatcg tgacctggga cgtgaaggat ttcgtggcgg cgacgccgat ctccgggccg
      841 gcgtgcatgt aaacgccgcc gtcgctttcg cgggcgatgg tgctgccgac gttgttgcag
      901 atgccgagcg tgcggtggcc cttgcgccgg ctttcgcgca gggcggcgag cgtgtcggcg
      961 gtttctccgc tctggctgac gacgaagacc agcgtgtcgc gggtcatggg aagattgcgg
     1021 tagcggaact cgctggcgaa ctcgcattcc accggaaggt gcgcgaggga ttcgatgagg
     1081 tattcgccga tcaaagccgc gtggaaggcc gtgccgcagg cgctgagcac gatgcgctcg
     1141 acgccgcgga gctcggcgtt ggtcatgttc agcccgccga gcttggcggt ggcttcctcg
     1201 ggggagagcc ggccgcgcat ggcgtcgaac acgctctgcg gctgctcgag catttccttc
     1261 agcatgtaat gcgggtaatc gcccttgctg acatcctccg cggtgaattc caccttgctg
     1321 atctggtaat ccgacccgct gcccgagagc gtggtgattt caaactccgc gccttgaatg
     1381 gcgacgattt cgtaatcgtt gagatacacg acgtcccgcg tgtggccgac gattgcgctc
     1441 acgtcgctgg ccaggaaatg ctggtcgcgg ccgatgccga gcacgagcgg gctgccgcgg
     1501 cgcgcgccga cgacgaaatc gggcaggccg gcgtgcacga cggcgatgcc gtaagtgccg
     1561 atgacgtgat gcagcgcgtc ccgcagggct tcgacgagcg agccccgggt cgtttccttc
     1621 cgccgttcga aggcccggcc gatgaggtgc gcgagcacct cggtgtcggt ctgggatttg
     1681 aacacatgcc cctccgcctg gagttcctcg cggagctgct ggtagttctc gatgacgcca
     1741 ttgtggacga gggcgagctt gccggactgg tcgaggtggg ggtgggcgtt ggcgtcggtg
     1801 acgccgccgt gggtggccca gcgggtgtgg ctgatgcccg cggagccggc gaccggctcg
     1861 cggccgatca ggtcggcgag gttggcgatg cggccaacgg ttttgcggac atggatgtct
     1921 ccaccggcca gcagggcgac gccggaggag tcataaccgc gatattcgag ccggcgcagc
     1981 ccgtccagaa ggatcggagt ggcctgggcc tttccaacgt aagcaacaat tccgcacatg
     2041 agtttttgta tcgtgacaaa ggcatggccc ggctgcaaga tgcaagctgc gacccgcatg
     2101 aagcgccctc tcacccctcc tttccaacga actctcgcca tcgtcatggg tggcggagtc
     2161 ggttcccgac tattcccgct gaccaaggaa cgcgccaagc ccgccgtccc gctgggcggc
     2221 aaataccgcc tggtggacat cccgatcagc aactgcctga actccggcat ccggagcatc
     2281 tacgtgctga cccagttcaa cagcacgtcg ctgcaccgcc acatcaacgc gagttacaag
     2341 ttcgaccatt tcagcccgag tttcgtggaa attctggccg cccagcagac gccggaaggc
     2401 tcgagctggt atcagggaac ggccgacgcc gtgcgccaga acatgcgcta ctttttggag
     2461 cagccgtatg attatttcat cattctcagc ggcgatcagc tctaccggat ggattaccgc
     2521 gagatcctcc gccagcacgc ggagtccgaa tcggacatca cgatcgcgac gatcccggtc
     2581 aaccgcgatg ccgcgaagga tttcggaatc atggacacgg atgccaccaa gcgcatcgtg
     2641 aaatttgtcg agaaaccgaa ggaggatgtc gtgctggacg gcttgcgcat tcccgaagcc
     2701 gtgctggagt ccatcggcaa atccccggat gaggatctgt atcaagcctc catgggcatt
     2761 tacgtgttca accggcaggt gctttgcgat tgcctggaca acgacctcgt ggacttcgga
     2821 aagaacatca ttccgaaggc gatcagcgac tacaaggtgg atgcctttat ttttcaaggc
     2881 tactgggagg acatcggaac catccgggca ttcttcgacg cgaatctcaa cctgacctcc
     2941 gaccagccgg aatattcctt ctacgaggcg ggggcgccga tttataccca tccgcgttac
     3001 ctgcccgcga gcgtggttcg ggacacccag atcaaccgtg cgatcatctc ggacggctgc
     3061 atcattgatt gcgaatccat cgagcgctgc gtgatcggcg tgcgcagcgt gattcagtcg
     3121 ggcacgaaga tcagcaacgc catcatcatg ggcgcggata tttacgagcg cgacctgccg
     3181 gaacccgttt tcgggcagcc ggcgatcggc atcggcaagg actgtgtgat ccagaatgcg
     3241 atcattgata aaaacgcccg ggtcggcaac caggtggtca ttacttccga gggcaaggag
     3301 aaggatctcg acggcgacgg cttctacgtt cgcgaaggaa tcgtggtggt gccgaaaaat
     3361 agcgtgattc ccgatggaac ttggatttaa ctgcgcggcg agatgaggag tttgttactg
     3421 ctggccgtcg tgctgttgtt gcccgggtgt ttgtttgaaa aacccatcac cgggccatcc
     3481 cgcagcatca atacatggat gcttggcgtc tgggagtttc ccaaaaaaaa tggcgagcgg
     3541 ctccaggcga tggtcgttcc gaaggacaac gatcattacg cgatttattt gcgcgaattc
     3601 accaagggcc ggaagttaaa gtccatcaag gaatacgacg cctggatttc gcgcgtcgga
     3661 ctggtcagtt tccttacggt gaaggtggac ggccaatatt gccccgtgca tttccaattg
     3721 ctcagccccg tcgagatccg gctgcggctt ccggattttg atccggggag cgaggcgctt
     3781 accccgtatg aactccgcca gcaggtgcgc cgcaaattca aggaaggcac gctgttgacg
     3841 aaggaagggg aaatctggac caaggtatcg gaaatttact ggccggcgga gggaaacccc
     3901 ggcgaccagc cggcgaacca gccgcttcgc cacgtgccgc agcccgctca gatggtgggg
     3961 caatagagcg attctcaaaa gagatttccg aaatacccaa cgggttgtag aggcgcttgt
     4021 cgggtgggga aatggggtcc cgccgcccca ttgggcgcct ttgtttgcgg ggaaacgggg
     4081 accccgcatc cccgtttacg ctgggcggtt ggcacaaccg cccctacatt gatccctttt
     4141 cggaaatctc aatctaaccg ccggtgctgg aatggcggca gcgccgccgt ttggggcgcc
     4201 ctgggaaaag tccgccggat gcagcctcac gcgacgcgaa gggaaagaca ttttaagaat
     4261 ttcccttgat ctccgcggct tggcgtctcc gcgtgaaaaa gggcttttct caggccacct
     4321 tggcgtcctt tttggcgccg tccttcttgt cgtccttttc ccggcgggga taagcgatgc
     4381 gcgaatgcat catgctctgc agggtcaggg tgaggctctt ggaaatgcgc tgaatttcct
     4441 ccagggtcag cggcgcttcg tcgagttggt ggtcctccag gcgggattcg acgagggact
     4501 tgaccagatc ttcaatgcgc tgcggcgtgg gccgttccag gcttcgggag gcgctttcca
     4561 ccgcgtcggc caggctgagg atcgccgtct ccttggtctg tggccggggg ccgggataac
     4621 ggaaatttcc ctcgctgacc tggggaatgt cctccggccg gatgttcatg atcttgccgc
     4681 cgagctccgc gtccttttgc tgctgccgcg cgcgctggta aaaataaccc accagcgagg
     4741 tgccgtggtg ctggcggatg ccgtccacga tgaccgaatt caatttatgc ttgagggcga
     4801 gatccacgcc ttccttgacg tgggcgatga tgatgagcgc gctcatcgtc ggcgtcagct
     4861 catcgtgggg attctcgccg gccggcatgt tttcggtgaa ataatccggc ttcaccagct
     4921 tgccgatgtc gtggaaatac gcggatacgc ggcagatggt cggattcgcc ccgacccgct
     4981 cggcggcggc ctcggcgagg ttggccacgg cgaggctgtg atggtaggtg ccgggcgctt
     5041 ccaggctcag gcgcttgagg agggggtggt tgaggtcggc catttccagc caggaaatat
     5101 ccgtcgtgac gccgaagatc gtttccagca acggcagcag gccggtgacg acgatggccg
     5161 ttccgatgcc cacgcccagg gcggccacgc tttgccagcc gatcatttgc caatccgtcg
     5221 aaggcagcga ttcccagatg
//
LOCUS       DATDAY010000275         5452 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_1911156, whole genome shotgun sequence.
ACCESSION   DATDAY010000275 DATDAY010000000
VERSION     DATDAY010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 5452)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5452)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5452
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1911156"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..314)
                     /locus_tag="VIS74_05475"
     CDS             complement(<1..314)
                     /locus_tag="VIS74_05475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792728.1"
                     /translation="MDKTRLLEQIAASRNALQADCKNLRDEWNFSQKAAQAVRSKPVK
                     WMGGALLSGYLLAKLRGRPKPAKRGKSGAVNAAKAARVKKFGFWAALVALVRFLAPLL
                     RPA"
     gene            complement(292..696)
                     /locus_tag="VIS74_05480"
     CDS             complement(292..696)
                     /locus_tag="VIS74_05480"
                     /inference="COORDINATES: protein motif:HMM:NF018980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEY5792729.1"
                     /translation="METQGPIGSLVALLGSAGKHLELLAALLAQEGREAGGRLIRALV
                     LLAAGLLLAGLGYIFLVLGLAFVLAAVFQWPWLWIALGLGLAHLAAAAFLLKRMSHYL
                     KVPAFSGFRAEVQKDLDLLHKNLPTHGQNPAA"
     gene            complement(704..1111)
                     /locus_tag="VIS74_05485"
     CDS             complement(704..1111)
                     /locus_tag="VIS74_05485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792730.1"
                     /translation="MSEEEQSKSILAKEKLESGTAHAKAALDATAEAAKAAAESVKQQ
                     AQGAYEVGKDHISKAATDFGDAATAAYEDIRTHAKQTAGEYRAKAETVWSDASAKVKN
                     FQSETEEYVREQPLKAVGLAFLGGLILGLISRR"
     gene            1229..1609
                     /locus_tag="VIS74_05490"
     CDS             1229..1609
                     /locus_tag="VIS74_05490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792731.1"
                     /translation="MKLPPLLGILLACSLPVLAQSEAQKIENTGEKVATEVKEAPAKI
                     EQTVTKEAAEVKADSADGQSKLKQGAGQAWEATKAATKNAAGAALNGTAHVADKVTEG
                     LEKAAESLKQAAHKLEGEKEDPKE"
     gene            complement(1633..1992)
                     /locus_tag="VIS74_05495"
     CDS             complement(1633..1992)
                     /locus_tag="VIS74_05495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY5792732.1"
                     /translation="MQNLSSQKPFVTKDGSRIRSILDRTNAPVENQSLAEARLAAGGA
                     TQRHYHKISEEFYFLLQGEGIMEIDGETRKVAPGDAILIPPGAWHQITAASDLQFLCC
                     CAPPYSHEDTFFEQTRG"
     gene            2013..2819
                     /locus_tag="VIS74_05500"
     CDS             2013..2819
                     /locus_tag="VIS74_05500"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY5792733.1"
                     /translation="MIFFEGCTALITGASAGLGTEFARQLAPYARTLVLVARRQERLE
                     LLTSELTGLFPNLQVRIYPADLSSESQRETFIRWLGEQNLEVDFLINNAGLGDRGKFA
                     TSEWDKVRQILDVNIAALTHLTHALVPGMAARGRAAILNVSSVAGFFPLPNFAVYAAT
                     KAYVTSFTEALRLELRPKGISITALCPGPVPTEFFDVAARGGGARPHYESIPAFAATP
                     QEVVSDALRAVSKDRPRVIPNPLLSFAVGLALLLPFFIVRRVLASKAVRR"
     gene            2871..3908
                     /locus_tag="VIS74_05505"
     CDS             2871..3908
                     /locus_tag="VIS74_05505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEY5792734.1"
                     /translation="MSNFTKPPAPADVLKGADAFREAVQLVNPQLYSVEERIQRQAKA
                     FDPAMEGYMSYVVDSGGKRLRPVLALLSGGATGKLTSSHVDLAVIVELIHISSLVHDD
                     IMDGADLRRDQPTANAKWGNSLSVLLGDALFAHALRLSTYFENNTISRRISDATVEVC
                     SGEILQTQRRFDLKLNLTDYYKIIEMKTAALFAVSCELGAFLSGAAPEVILAMREFGN
                     KLGIAYQIYDDCVDIAGKEEDAGKTLGTDLQKGKFTLPIILLLQRAPEPVRRQIQEML
                     LNEEFFDAGILGGHIHRIGAMKAAVATATQLIEDARARLREVPNNHFSKALDSVAAYI
                     SSLLAPLGSDA"
     gene            3973..4674
                     /gene="radC"
                     /locus_tag="VIS74_05510"
     CDS             3973..4674
                     /gene="radC"
                     /locus_tag="VIS74_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HEY5792735.1"
                     /translation="MPSSRLRELPESERPREKLAGLGAASLTDAELLAIFIRTGLRGR
                     NAVGVAQDLLRQRGSLLELSRCTIRELMNSAKGIGLAKACELAAAFELGKRLARGLGA
                     RPKVDSPEAIYDLMAPEMQALRTESLRLLLLDTKYQLIRVEEISLGSVSESIAHPREI
                     FRPALIHSASAVVLVHNHPSGDPEPSRADHQTTRRLLEAAEILGVRLLDHVVIGSPEG
                     GRKAYASFREMGVIG"
     gene            4875..>5452
                     /gene="lpxA"
                     /locus_tag="VIS74_05515"
     CDS             4875..>5452
                     /gene="lpxA"
                     /locus_tag="VIS74_05515"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: protein motif:HMM:NF003657.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HEY5792736.1"
                     /translation="MKIHPTALVEEGAQLADDVEIGPYALIGPEVVIGAGCKIQARAV
                     LTSKVTLGERNLIGYGSVIGAAPQDFAHTDSIPSEVRIGNGNTIREYVTIHRGSQPDT
                     ATVIGNECYVMTGCHFAHNVQIGNGVILANNSLFAGYASVQDHAVLGGGSVFHQFMRI
                     GKYAMTRGGTRFSKDVPPYTTADDRKVVGLNI"
BASE COUNT         1026 a         1801 c         1628 g          997 t
ORIGIN      
        1 gccggccgga ggaggggagc cagaaaacgg accagcgcca ccagcgcggc ccagaatcca
       61 aattttttca ccctggccgc cttcgccgca ttcacggcgc cggatttgcc ccgcttcgcc
      121 ggcttcggcc ggccgcgcag cttggccagc agatagccgg aaagcagtgc tccgcccatc
      181 cacttgaccg gcttggaccg cacggcctgc gcggctttct gggaaaaatt ccattcgtcc
      241 cgcagatttt tgcaatctgc ctggagggca ttccgcgatg ccgcgatttg ctcaagcagc
      301 cgggttttgt ccatgagttg ggagattttt gtggagcaga tcgagatcct tctgaacttc
      361 cgcccggaaa ccggagaagg ccggaacctt gagataatgg ctcatccgct tcaacagaaa
      421 tgccgcggcg gcgaggtgcg ccaggcccag gcccagcgcg atccagagcc agggccattg
      481 aaaaacggcc gccagcacga aagccaggcc gaggaccaga aagatgtaac ccagcccggc
      541 cagaaggagg ccggccgcca gcagaaccag ggcacgaatg agccgcccgc ccgcctcgcg
      601 gccttcctgg gccagcaagg cggcaagcaa ttccaaatgc ttgcctgccg agcccaggag
      661 cgccaccaag ctgccaatcg gaccttgggt ttccacttcc gggctaacgg cgggaaatca
      721 accccaggat caatccgccc aagaaggcca ggccaaccgc cttgagcggt tgttcgcgga
      781 cgtattcctc ggtttcggat tgaaaattct tcaccttcgc gctggcgtcg ctccaaaccg
      841 tctccgcctt ggcgcgatat tcacccgccg tctgcttggc atgggtgcgg atgtcttcgt
      901 aggcggccgt ggcggcatcc ccaaagtcgg ttgcggcttt cgagatgtgg tccttgccca
      961 cctcataagc cccttgggcc tgttgtttga cggactcggc cgcggctttg gcggcttccg
     1021 cggtggcgtc caaggcagcc ttggcatggg cggtcccgct ttccagtttc tccttcgcga
     1081 ggatggattt agattgttct tcttcagaca tgggtgattt cctcctgttc gctaggagca
     1141 tcgggcaagt aagccggaaa aacaagcctc tttgggtgac ggattttttg gggtggaact
     1201 tttccgcgtt ttacggaaca ttaaaaagat gaagctaccc ccattgcttg gcatcttgct
     1261 ggcctgctcc ctccccgtcc ttgcccaaag cgaggcccag aagatcgaaa ataccggtga
     1321 aaaagtcgcc accgaagtca aggaggcgcc cgccaagatc gaacagaccg tcaccaagga
     1381 agccgccgag gtgaaggccg attccgccga tgggcagagc aaactcaagc aaggcgccgg
     1441 ccaggcgtgg gaagcgacca aagcggccac caaaaacgcg gccggagccg cgctcaacgg
     1501 gaccgcccac gtggcggaca aggtcacgga aggcctcgaa aaagccgccg agagcctcaa
     1561 gcaggccgcg cacaagctcg agggagagaa ggaagatccc aaggaataga aaggccggga
     1621 aacaacggcc ccttatcccc gcgtttgctc aaaaaaggta tcctcgtggg aatacggcgg
     1681 ggcgcagcag cagagaaatt gcaggtccga ggcggcggtg atctggtgcc aggcgcccgg
     1741 cggaatcaaa atcgcgtcac ccggcgccac cttccgggtt tcgccatcga tttccatgat
     1801 gccctcgccc tggagcagaa aatagaattc ctcgcttatt ttgtgataat gccgctgtgt
     1861 cgcgccgccc gcggccagcc gcgcctcggc gaggctctgg ttctccaccg gcgcattggt
     1921 ccgatccaga atgctgcgga tccgggatcc gtccttcgtc acgaagggtt tttgactgga
     1981 taagttctgc atgatcatcg acatggtatt ccatgatatt ctttgaaggt tgcacagcct
     2041 tgattaccgg cgcctccgcc ggactcggaa cggagtttgc ccggcagctg gcgccttacg
     2101 cgcgaaccct cgtgctggtc gcgcgccgcc aagagcggct ggagctgctc acctccgaac
     2161 tcaccggcct ctttcccaat cttcaggtgc ggatctaccc cgccgacctc agttcggaaa
     2221 gccagcggga gaccttcatc cgctggctgg gggagcaaaa cctcgaggtg gattttctca
     2281 ttaacaatgc cggcctgggc gatcgcggca aattcgccac gagcgaatgg gacaaggtcc
     2341 gccagatcct cgatgtcaac attgccgcgc tcacccacct cacccatgcg ctggttccgg
     2401 gaatggcggc ccgcggccgg gcggcgatcc tcaacgtcag ctcggtcgcc ggatttttcc
     2461 cgctgcccaa tttcgcggtg tatgccgcca ccaaggccta cgtgacttcc tttaccgagg
     2521 cgctccgcct ggagctgcgc ccgaagggca tctcgatcac cgcgctttgc ccgggaccgg
     2581 tgcccacgga attcttcgac gtggccgccc gcggcggcgg cgcccggccg cattacgaat
     2641 ccattccggc tttcgccgcc actccgcagg aggtcgtcag cgacgcgctg cgggccgtct
     2701 ccaaggaccg cccccgcgtg atcccgaatc cgctgctctc cttcgcggtc ggcctcgccc
     2761 tgctccttcc gtttttcatc gtccggcgcg tgctggcttc caaagccgtc cgccgatgaa
     2821 ccttgccggg agcgttgaca agcgggcctg acggcagtca ttgtttgggc gtgagcaact
     2881 tcaccaagcc tcccgcgccg gccgacgtcc tgaagggcgc cgacgcgttc cgcgaagcgg
     2941 ttcaactggt caatccccag ctctacagcg tggaagagcg catccagcgg caggccaagg
     3001 cattcgatcc ggccatggaa gggtacatgt cctacgtggt ggacagcggc ggcaagcggc
     3061 tccgccccgt cctcgccctt ctctccggcg gcgccaccgg caagctcacc agcagccacg
     3121 tggatctcgc ggtgatcgtg gaactgatcc acatctcctc gctggtgcat gacgacatca
     3181 tggacggcgc cgacctgcgc cgggaccagc ccaccgcgaa cgccaaatgg ggcaattccc
     3241 tgagcgtgct gctcggcgac gcgctttttg cccacgccct gcggctttcc acctactttg
     3301 agaataatac catcagccgc cgcatctcgg acgccaccgt ggaggtgtgt tccggcgaga
     3361 ttctccagac ccagcgccgc ttcgacctga agctgaatct gaccgattat tacaagatca
     3421 tcgaaatgaa aaccgcggcg ctcttcgccg tctcctgcga gctgggggcg ttcctgagcg
     3481 gcgcggcccc cgaggtgatc ctggccatgc gggagtttgg caacaagctc ggcatcgctt
     3541 accagattta cgacgactgc gtggacatcg ccggcaagga ggaggacgcc ggcaagacgc
     3601 tgggcaccga tctgcagaag ggaaagttca ccctgccgat catcctgctg ctccagcgcg
     3661 cgccggaacc cgtccgccgc caaatccagg aaatgctcct gaacgaggaa tttttcgatg
     3721 ccggcatcct cggcgggcac atccaccgca tcggcgccat gaaggccgcc gtcgcgaccg
     3781 ccacgcagct gatcgaggat gcccgcgcgc ggctgcgcga ggtgccgaac aatcattttt
     3841 ccaaggcgct cgacagcgtc gccgcctaca tcagttcgct gctcgcgcca ctggggtcgg
     3901 atgcatgaac ggcgggaagg cttggcgcaa aacggatccc gcattccccc gatcccccac
     3961 ccaaggctga acatgccctc ctcccggctg cgcgaacttc ccgaatccga acgcccccgg
     4021 gaaaaactgg ccggactggg ggccgccagc ctgaccgacg ccgaactgct ggccatcttc
     4081 atccgcacgg ggctgcgcgg gcgcaacgcc gtcggcgtcg cgcaggattt gctgcggcag
     4141 cgcggcagtt tgctggagct ctcccgttgc accatccgcg agctgatgaa ctccgccaag
     4201 ggcatcggcc tggccaaggc gtgcgagctg gccgcggcgt ttgagctggg caagcggctg
     4261 gcccgggggc tgggcgcgcg gcccaaggtg gattcccccg aggcgatcta cgacctcatg
     4321 gccccggaga tgcaggcctt gcggaccgag tccctccgcc tgctgttgct ggatacgaaa
     4381 taccagctca tccgggtgga ggaaatttcc ctgggttcgg tcagcgaaag catcgcgcat
     4441 ccgcgggaga tttttcgccc ggcgttgatc cattccgcct ccgccgtggt cctcgtccac
     4501 aatcatcctt ccggcgatcc ggagcccagc cgggcggatc atcaaaccac ccgccgtttg
     4561 ctggaagccg cggaaatcct cggggtgcgc ctgctggatc atgtggtgat cggctccccc
     4621 gaaggcggcc gcaaggccta cgcgagcttc cgggagatgg gagtgattgg ttgatccgcc
     4681 tcgttggaga ttgttccacc gcggattacg cggatgaacg cggataatgt ccgtggaaag
     4741 gaggcctttg ccgtttctta tccgcgccaa tcccgtaatc cgcggtcgaa gtttcatttc
     4801 cggcagccag aggcaggtca aaagaatgtt tgcttccgtc cgttcgggaa catttggtag
     4861 gttcctcgcg tttcatgaag attcacccta cagccctcgt tgaggaaggc gcgcaattgg
     4921 ccgacgatgt cgagatcggc ccgtatgccc tcattggccc cgaggtcgtg atcggcgccg
     4981 gctgcaaaat ccaggcccgc gccgtgctca cgagcaaagt cactctcggc gaacgcaatc
     5041 tcatcggcta cggttccgtc atcggcgccg ccccgcagga tttcgcgcac acggattcca
     5101 ttcccagcga ggtgcgcatc ggcaatggca acacgatccg cgaatacgtg accattcacc
     5161 gcggctccca gccggacacc gccacggtca tcggcaacga atgctacgtc atgaccggct
     5221 gccacttcgc ccacaacgtc cagatcggca acggcgtcat cctcgccaac aacagcctct
     5281 tcgccgggta tgcctccgtg caggatcatg ccgtgcttgg cggcggcagc gtgttccatc
     5341 aattcatgcg catcggcaaa tacgccatga cccgcggcgg cacgcgcttc agcaaggacg
     5401 tcccgcccta cacgacggcc gacgatcgca aagtggtcgg tttgaatatt at
//
LOCUS       DATDAY010000276         4470 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_403027, whole genome shotgun sequence.
ACCESSION   DATDAY010000276 DATDAY010000000
VERSION     DATDAY010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 4470)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4470)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4470
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_403027"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(111..500)
                     /locus_tag="VIS74_05520"
     CDS             complement(111..500)
                     /locus_tag="VIS74_05520"
                     /inference="COORDINATES: protein motif:HMM:NF017868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase family protein"
                     /protein_id="HEY5792737.1"
                     /translation="MIRVFVYGTLMPGYRNHERFAAPHLISCRPATTQGKLYHLPAGY
                     PGVVEGEGIVHGKLLELRDEALPPLDELEAYDPAAAPEENEYYRREVSVALETGDGVN
                     AWIYFIALERVESMRGIRVESGHWRGE"
     gene            complement(497..1114)
                     /locus_tag="VIS74_05525"
     CDS             complement(497..1114)
                     /locus_tag="VIS74_05525"
                     /inference="COORDINATES: protein motif:HMM:NF036541.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DR2241 family protein"
                     /protein_id="HEY5792738.1"
                     /translation="MTTIRELLADYLARGAFVIGQVRVTPELELRHVADEAVKNLSPH
                     SGPAAARFLAVYDDAGKYRPLKTAPNLRRGWSLQLASIPELQLALDAFYPAALGTWLA
                     FRQNQLVPVPLRATLARQTGMYRIVQSLADSQITKLVAATCAPEFTLALSLWEIEPGK
                     PHAFTRPRGQVEAGVPADVIPLLSAEAGNGIVAEGRKIVKAAPQS"
     gene            complement(1275..1685)
                     /gene="atpC"
                     /locus_tag="VIS74_05530"
     CDS             complement(1275..1685)
                     /gene="atpC"
                     /locus_tag="VIS74_05530"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HEY5792739.1"
                     /translation="MATLRLEIVTPEAKAYSDDVDSVVIPGAEGELGILPEHSPLMTQ
                     LLPGELRVMKAGKELRLAVGEGFVEITPDKVAVLTDMAIEEASIDETLAEQAMRSAEE
                     ALKTQHISSEEHAALQASFKNSLAQLRVKRRRPL"
     gene            complement(1776..3191)
                     /gene="atpD"
                     /locus_tag="VIS74_05535"
     CDS             complement(1776..3191)
                     /gene="atpD"
                     /locus_tag="VIS74_05535"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEY5792740.1"
                     /translation="MITTKGKVIQVIGPVIDAEFNPADGPLPKIYDALEIDFISAGQA
                     TKLTLEVQQHLGENWVRAIAMSTTEGLVRGMEVINTGAPISVPVGEAVLGRIFNVTGE
                     PTDELGPVKATKKYPIHRPAPALIEQDTQAKILETGIKVIDLICPFTKGGKVGAFGGA
                     GVGKTVVIMELINNIAKGHGGYSVFAGVGERTREGNDLYHEMADAGVIRLDKIEESKV
                     ALVYGQMNEPPGARLRVALSALAMAEYFRDEKNQDVLLFIDNIFRFSQAGSEVSALLG
                     RTPSAVGYQPTLAAEMGQLQERITSTTKGSITSFQAVYVPADDLTDPASANTFAHLDS
                     TIVLERSIAELGIYPAVDPLASTSKALAPEIIGEEHYRVARGVQKVLQRYKDLQDIIA
                     ILGMDELSPEDKLTVYRARKIQRFLSQPFHVAEVFTGTPGQYVPIAETIRGFKEILEG
                     KYDDVPEGNFYMKGTIDQIKD"
     gene            complement(3212..4081)
                     /gene="atpG"
                     /locus_tag="VIS74_05540"
     CDS             complement(3212..4081)
                     /gene="atpG"
                     /locus_tag="VIS74_05540"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HEY5792741.1"
                     /translation="MANLRDIRRRIKSVKNTAQITKAMQMVAASKMRKAQQAALNGRP
                     FAELLNRVLVSLRDRVDSALHPLLAKREVRNELVILLSTDKGLCGGLNTNLFREASRY
                     DAATTKFISVGRKGTQFLARTKRNLIADFPLKDHPNFLETKAISKLAIQKFLAGEVDK
                     VTVLFPRFVNTLVQTPTVIPLLPITSLEEIGLSGGEFTPLKTGDAIITFEPNPEEVLD
                     SILPFYTHFELFQFLLGTRASEQSARMVAMKSATDNAKTLVKDLTLDYNKIRQASITS
                     ELLEITTAQMALE"
     gene            complement(4083..>4470)
                     /locus_tag="VIS74_05545"
     CDS             complement(4083..>4470)
                     /locus_tag="VIS74_05545"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HEY5792742.1"
                     /translation="TKEYAKRIVRLVDSLPDSISGRTLASQVMRSGTSVAANYRSLCR
                     SKSLPDFINKTSIIEEEADETCFWLELIVETGKLPVTKVAPLLKESNELVSIFVASRR
                     TARKRDNTRKSRWGKGPHPLTVETRK"
BASE COUNT          848 a         1313 c         1381 g          928 t
ORIGIN      
        1 gcgaattcac tggtggttta cgaggaaaag cagctcaaaa tccacgacaa ggcggccaag
       61 cggaaggctc ccgcgctgga gtggtcgatc tgagcgggcg gccgcgggat ttattcgccg
      121 cgccaatgcc cggattccac ccggatgccg cgcatggatt ccacccgttc cagcgcgatg
      181 aaatagatcc acgcgttcac gccgtctccc gtctccagcg ccacggaaac ctcgcggcgg
      241 taatattcgt tttcctccgg cgccgccgcg ggatcataag cctccaactc atcgagcggg
      301 ggcagcgcct cgtcccgcag ctccagcaat ttcccgtgaa cgatgccttc gccctccacc
      361 acgccgggat accccgcggg cagatgatat aactttccct gtgtggttgc cggacggcag
      421 gaaatcaaat gcggcgcggc aaaccgctcg tggttccgat aacccggcat cagcgtcccg
      481 taaacaaaca cccggatcat gactgcggcg ccgccttcac gatttttcgc ccctcggcga
      541 cgatcccatt gccggcttcg gcggaaagca ggggaatgac atcggcgggc acacccgcct
      601 cgacctgccc gcgcgggcgg gtaaaggcat gcggtttccc cggttcgatc tcccaaaggc
      661 tgagcgccag cgtgaattcg ggagcgcagg ttgccgccac aagcttggtg atttgtgaat
      721 cggcaaggct ttgcacgatt cgatacatcc ccgtctgccg ggccagcgtg gcgcgaagcg
      781 ggaccggaac caattggttc tgccgaaacg ccagccaggt tcccagggcc gccggataaa
      841 aggcgtccag cgcgagctga agttccggaa tcgacgcgag ctgcaagctc cagccgcgcc
      901 ggagattcgg cgcggttttc agcgggcggt attttcccgc gtcatcgtaa accgccagaa
      961 accgcgcggc agccggtccg gaatgtggcg ataaattttt cacggcctca tcggccacgt
     1021 gccggagttc gagttcgggc gtcacccgaa cctggccaat gacaaacgcc ccccgcgcga
     1081 ggtaatccgc cagcagctcg cgaatggtgg tcatttccca acctaagcgc attttcgatt
     1141 taaatgaaaa tcaatccccg gacgcaaact acggcgaccc cgccctagct atcccgggta
     1201 gggacggctc gccgaggcgt ccgtgatttt tctcatgcgc ggcctaaacg gaagcaattc
     1261 cgctgccttg tgaatcaaag cggacggcgg cgcttgaccc gcagctgcgc cagcgaattc
     1321 ttgaacgaag cctgcagcgc cgcgtgctcc tcggagctga tgtgctgggt tttcagcgcc
     1381 tcctcggcgc tgcgcatcgc ctgctcggcc agcgtctcat caatgctggc ctcctcgatc
     1441 gccatatcgg tgagcacggc caccttgtcc ggagtgattt ccacaaaccc ctcgccgacg
     1501 gccaggcgaa gttccttgcc cgccttcatg acgcgcagtt cgccgggaag cagctgggtc
     1561 atgagcgggg aatgctcggg cagaatgccg agctccccct ccgctccggg aatgacgacg
     1621 ctatccacgt catccgaata ggctttcgcc tccggagtga cgatttcaag tctgagtgtg
     1681 gccatgagat tttcgagtgt cgattttcga ttctcgaatg gccgatcgga cgccgcagtt
     1741 ccaatcgaaa atcaaaaatc gaaaatcgaa aattcctagt ccttgatctg gtcgatcgtg
     1801 cccttcatgt agaaattgcc ttccggcacg tcgtcgtatt tgccttcgag gatttccttg
     1861 aagccgcgga tggtttccgc gatcggcacg tattggcccg gagttccggt gaagacttcg
     1921 gcgacgtgga atggctggct gaggaaacgc tggattttgc gcgcgcgata caccgtgagc
     1981 ttgtcctcgg gcgagagttc gtccatgccg agaatcgcaa tgatgtcctg caggtccttg
     2041 tagcgctgga gcaccttctg gacgccgcgg gcgacgcggt agtgctcctc gccgatgatt
     2101 tccggcgcga gcgccttcga ggtcgaagcg agcgggtcca cggcgggata aatgccgagt
     2161 tccgcgatcg aacgctccag cacgatggtc gaatccaggt gcgcgaacgt gttcgccgac
     2221 gccgggtcgg tcaagtcgtc cgcgggcacg taaacggcct ggaacgaggt gatggagccc
     2281 ttcgtcgtcg aggtgatgcg ttcctggagc tggcccattt ccgccgcgag cgtgggctgg
     2341 taacctaccg cggacggcgt gcggcccagc agcgcggaca cttcggaacc ggcctgcgag
     2401 aagcggaaaa tgttatcgat gaacaggagc acgtcctggt ttttttcgtc gcggaaatat
     2461 tccgccatgg ccagggcgct gagcgccacg cggagacggg cgcccggagg ttcgttcatc
     2521 tggccgtaaa ccagcgccac cttggattcc tcgattttgt cgagcctgat gacgcccgca
     2581 tcggccattt cgtggtagag atcgttgccc tcacgggtgc gctcgcccac gccggcgaac
     2641 acggagtaac ccccgtggcc cttggcaatg ttgttgatga gctccatgat gacgaccgtc
     2701 ttgccgacgc cggcgccgcc gaaggcgccc accttgccgc ccttggtgaa gggacaaatg
     2761 aggtcgatga ccttgatgcc ggtttcgagg atcttcgcct gcgtgtcctg ctcgatcagc
     2821 gcgggggcgg gacgatgaat cgggtatttc ttcgtggcct ttaccggacc gagctcgtcg
     2881 gtcggctcgc cggtcacgtt gaagatgcgg ccgaggacgg cttcgccaac gggaaccgag
     2941 atcggcgcgc cggtattgat gacctccatt ccgcgaacga ggccttcggt cgtggacatc
     3001 gcaatggcgc ggacccagtt ttcaccgagg tgctgctgca cttcgagggt cagcttcgtg
     3061 gcctggccag cactgatgaa atcaatctcc aatgcgtcgt aaattttcgg cagcggaccg
     3121 tcggcgggat tgaactccgc atcaataacg ggcccgatga cctggatgac ttttcctttg
     3181 gtggtgatca tggttgggtg gattcctgga tttattcgag agccatctgg gcggtggtga
     3241 tttccagcaa ttcgctggtg atgctggcct gacggatttt gttgtagtcg agagtgagat
     3301 ccttcacgag ggtcttggca ttgtcggtgg cgctcttcat ggccaccatg cgggcgcttt
     3361 gttccgaggc gcgggtgccg agaagaaatt ggaagagttc gaagtgcgta tagaaaggca
     3421 ggatggaatc gagcacttct tccggattcg gctcgaaggt gataatggcg tcgccggttt
     3481 tcagcggggt aaattcgccg cccgaaaggc cgatttcctc aaggctcgtg atcggaagga
     3541 gcggaatgac cgtcggggtc tggaccagcg tattgacgaa acgcggaaag agcacggtga
     3601 ccttgtccac ctcgcccgcg aggaactttt ggatggcgag cttgctgatc gccttcgtct
     3661 ccagaaaatt cggatgatcc ttcaggggaa aatccgcgat cagattccgc ttggtccggg
     3721 cgagaaattg cgtgcccttg cggcccacgg aaatgaattt cgtcgtcgca gcgtcgtaac
     3781 gcgaggcttc ccggaagaga ttggtgttca ggccgccgca aagccccttg tcggtgctga
     3841 gcagaatcac gagctcgttc ctgacctcgc gcttggccag cagcggatgc agcgcggagt
     3901 ccacgcgatc ccgcagcgag acgagcacgc ggttgagcag ctcggcaaac ggccggccat
     3961 tgagcgcggc ctgctgggcc ttgcgcatct tggacgcggc caccatctgc atcgccttgg
     4021 taatctgggc ggtgttcttg accgacttga tgcggcggcg aatgtctcgg aggtttgcca
     4081 tgttattttc gagtttcgac tgtaaggggg tggggcccct taccccaccg ggattttcga
     4141 gtgttgtcgc gcttccgcgc ggtgcggcgc gaggcgacga agatagagac caactcgttg
     4201 gattccttca ataatggcgc aaccttggta acgggcaatt ttccagtttc gacgatcagt
     4261 tcgagccaga agcaggtttc gtcagcttct tcctcaatga tcgatgtctt gttgatgaaa
     4321 tcaggcaatg actttgaacg gcagagcgag cgataattcg cggcaaccga agttccgctg
     4381 cgcatcacct gagaggccag cgtccggccc gaaatcgaat cggggaggga atccactaga
     4441 cggacaatcc gtttggcata ttccttcgtt
//
LOCUS       DATDAY010000277         3443 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_2578761, whole genome shotgun sequence.
ACCESSION   DATDAY010000277 DATDAY010000000
VERSION     DATDAY010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 3443)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3443)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3443
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2578761"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            88..744
                     /locus_tag="VIS74_05550"
     CDS             88..744
                     /locus_tag="VIS74_05550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792743.1"
                     /translation="MAEPDNEFDAPEIWDEYQWERFLQQQDRTTEKYFQLMEQYLDHP
                     DRDRLIAQEMGWSLAMESEEDFSADAFMDQLEEENDSESEPTDEEYERFARSPIYQDT
                     LKLHRWIHQWMEKRPDLQEDENAIRLATQAAIAGAKLAAALCGQEDAELGMTIAYLKR
                     GLKAMNEALEAGANLGREGLLPPRRLASLNQRLFRVRNQIVDLMRAYRIEWKNRYGDA
                     "
     gene            741..914
                     /locus_tag="VIS74_05555"
     CDS             741..914
                     /locus_tag="VIS74_05555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792744.1"
                     /translation="MSPAVRCLLAGALCAVAAAALTVLCAGTIFDKQLGRAENQILSF
                     EVLRPELKLEIQP"
     gene            911..1246
                     /locus_tag="VIS74_05560"
     CDS             911..1246
                     /locus_tag="VIS74_05560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792745.1"
                     /translation="MTLVRVGAFFLVWMASTLAEDRVQCVEMDAITQISVKSKSRPLV
                     PGTTQWLKPGGPHAFKVECLERQPRLYRLKLELYESGQALAAAEVLIGRDQPITLRSP
                     EKTWRVWVE"
     gene            complement(1480..3114)
                     /locus_tag="VIS74_05565"
     CDS             complement(1480..3114)
                     /locus_tag="VIS74_05565"
                     /inference="COORDINATES: protein motif:HMM:TIGR00813.1"
                     /note="Members of the Solute:Sodium Symporter (SSS), TC
                     2.A.21 as described in tcdb.org, catalyze solute:Na+
                     symport. Known solutes for members of the family include
                     sugars, amino acids, nucleosides, inositols, vitamins,
                     urea or anions, depending on the system; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/solute symporter"
                     /protein_id="HEY5792746.1"
                     /translation="MHFSPLLAAAQSPTALLMFLAFVGATLVITYYSARRSKGSSAYF
                     AAGRRITGWQNGLAVAGDYMSAASFLGISGMIAFFGYDGFMYSVGFLVAYLTVLFIVA
                     EPLRNAGKYTMADLLAYRLQQKPVRAMASLSTLTVSTFYMIAQMVGAGALVKLLLPGV
                     SFEIAVVGVGVLMIIYVVFGGMLATTWVQIIKAILLMAGSLFLSILIMKHFGFSFSNF
                     FTAISHVTYVDKGVEVTRDFLQPGLKFGSGWGPLDLVSLGLALVFGTAGLPHVLVRFY
                     TVPNAPTARSSVVWAMLIIGLFYIMTTFLGFGAASILTPANIVMDGKPNDNMAAPLLA
                     QFLGGEIFFAFISAVAFATILAVVAGLTISASTSFAHDLYTNVIHHGRETHPGEEVKV
                     ARITAFVVGAIAIILAIQLQTINVAFLVGLAFAVAASANLPVIVLSIFWRRFTTAGAV
                     AGLAVGLVSSIVLIILSPSIMGIDGPEVAAAKRHLIQAAPLFPLANPGIVSIPLGFVA
                     AIVATLLSREPEAEAKFTELTVRANTGLGAEKATEH"
     gene            complement(3120..>3443)
                     /locus_tag="VIS74_05570"
     CDS             complement(3120..>3443)
                     /locus_tag="VIS74_05570"
                     /inference="COORDINATES: protein motif:HMM:NF016244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HEY5792747.1"
                     /translation="SSAPASSAPAVNWNEIASDPDFKKLVASKLRFIIPATVFFVVYY
                     FALPVLVGWFPELMKTRVAGSVNFAYLFALSQFFMAWILAFLYVGVAAGWDKRAAALL
                     AKFKH"
BASE COUNT          768 a         1028 c         1096 g          551 t
ORIGIN      
        1 cgggcagttg cgcctacaga aacagcgcga agaggaacgc gaaaaatcct ttgccagcgc
       61 cccggaagcg gtttaggaag ggaataaatg gcggaaccgg acaacgagtt tgatgccccg
      121 gaaatctggg acgaatacca atgggagcgt tttctccagc agcaggatcg caccacggag
      181 aagtattttc agctcatgga gcaatacctc gaccacccgg accgcgaccg cctcatcgcc
      241 caggaaatgg gctggtcgct ggccatggaa tccgaagagg atttttccgc ggatgccttc
      301 atggatcaat tggaggagga gaacgacagc gaaagcgagc cgaccgatga ggaatatgaa
      361 cgcttcgccc gttcgccgat ttatcaggac acgctgaagc tgcaccgctg gattcaccag
      421 tggatggaaa aacgccccga cttgcaggag gacgagaatg ccatccggct cgcaacccag
      481 gcggccatcg ccggagccaa gcttgccgcc gcgctctgcg ggcaggaaga cgccgagctg
      541 ggcatgacca tcgcctacct caagcgcggg ctcaaagcca tgaatgaggc actcgaagcc
      601 ggcgcgaacc ttggccgcga agggctgctg ccgccccgcc ggctggcctc gctcaaccag
      661 cgcctcttcc gcgtgcgcaa ccagattgtc gatctcatgc gagcgtatcg catcgagtgg
      721 aagaaccgct acggagacgc ttgagtcccg ccgtccgctg cctgctggcc ggagcgctct
      781 gcgccgtggc cgccgccgca ttgaccgtcc tgtgcgcggg caccattttc gacaagcaac
      841 tgggccgggc ggaaaatcag attctctcct tcgaagtgct ccggccggag ctgaagctgg
      901 agatccagcc atgaccctcg tccgagtcgg cgcgtttttt ttggtatgga tggcgtccac
      961 cctcgcggag gatcgcgtgc agtgcgtgga aatggacgcc atcacccaga tttccgtgaa
     1021 gtccaagtcc cgcccgctcg ttcccggaac cacccaatgg ctgaaacccg gcggcccgca
     1081 tgccttcaag gtggaatgcc tggaacgaca gccgaggtta taccggctga aactcgaact
     1141 ttacgaatcc ggccaggcgc tcgccgccgc cgaggtgctg atcgggcggg atcagccgat
     1201 caccctgcgt tcgccggaga agacctggcg ggtatgggtg gagtagggga atttgtggta
     1261 aggcgaggtc gatgaccccg ccgtgacttg ctataaaaat cgaaaaaatt ctccgcgcgg
     1321 gaggcgaaat gaaaagcgga gccttacacg cctgcccgat gccaaatgcc ccgagcggaa
     1381 cgtccgaaac gccagcgctt atcaactcct catttaattt agggcaacgg gtcgggtgag
     1441 gataagtgca atccgcaagc cgggcgagcc ggaactgaat caatgttccg tcgccttttc
     1501 cgcgcccagg ccggtgttgg cccgcacggt cagctcggtg aacttcgcct cggcttcggg
     1561 ttcgcggctc agcagcgtag ctacgatcgc ggccacaaag cccagcggaa tgctcacgat
     1621 gcccggattg gccagcggaa acagcggcgc ggcctggatc aggtggcgct tcgcggcggc
     1681 gacctcgggc ccgtcgatgc ccatgatgga cgggctcagg atgatcagca cgatgctcga
     1741 gaccaatccg accgccagcc ccgccaccgc gcccgcggtc gtgaagcgcc gccagaaaat
     1801 gctcagcacg atgaccggca gattcgcgct cgccgccacg gcaaacgcga ggccgacgag
     1861 gaaggcgaca ttgatggttt gaagctggat cgcgaggata atggcgatcg cgccgacgac
     1921 aaaggccgtg atccgcgcca ccttcacttc ctcgcccgga tgcgtctcgc ggccgtggtg
     1981 gatcacgttg gtgtagaggt cgtgcgcgaa cgacgtgctc gcggaaatcg tcagtcccgc
     2041 gacgacggcc aggatcgtcg cgaaggccac ggcggaaatg aacgcgaaga aaatttcccc
     2101 gccgaggaat tgcgccagca acggggcggc catgttgtca ttcggcttgc cgtccatgac
     2161 gatgttcgcc ggcgtcagga tcgaggccgc gccgaagccg aggaaggtcg tcatgatgta
     2221 gaacagcccg atgatgagca tcgcccagac cactgaactg cgggcggtgg gcgcattcgg
     2281 cacggtgtaa aagcggacca gcacatgcgg cagtccggcg gttccaaaaa ccagcgccag
     2341 gccgagcgag acgagatcga gcggccccca gccggagccg aatttcagcc ctggctgaag
     2401 gaaatcacgg gtgacttcca cgcccttgtc cacgtaggtc acgtgggaaa tcgcggtgaa
     2461 gaaattcgaa aagctgaagc cgaagtgctt catgatgaga atgctcagaa acaggcttcc
     2521 cgccatgagc agaatggcct tgatgatctg cacccacgtg gttgccagca tgccgccgaa
     2581 gaccacgtag atgatcatga gcacgccgac gcccacgacg gcgatctcga agctcacgcc
     2641 gggcaggagg agcttgacca gcgcgccggc gccgaccatt tgcgcgatca tgtaaaacgt
     2701 cgaaaccgtc agcgtgctga gcgaggccat tgcccgaacc ggcttttgct ggaggcgata
     2761 ggcgagcagg tcggccatcg tgtatttgcc ggcattgcgc aggggctcgg cgacgatgaa
     2821 gagcacggtc agatacgcga ccaggaaacc gacggaatac atgaaaccgt cgtatccaaa
     2881 aaacgcgatc attcccgaga tgcccagaaa gctggcggcg ctcatgtaat cgcccgcgac
     2941 ggcgaggcca ttctgccatc cggtaatccg gcggccggcc gcgaagtaag cgctggagcc
     3001 cttcgagcgg cgggcgctgt agtaggtgat gaccagcgtg gcgcccacga acgccagaaa
     3061 catgaggagc gccgtggggc tctgggcggc ggcgagaagc ggggaaaaat gcatcgcggt
     3121 taatgtttga atttcgccag cagcgcggcc gcgcgtttgt cccagcccgc ggcgacgccc
     3181 acgtagagga acgcgagaat ccacgccatg aaaaattggg agagcgcgaa caggtaggca
     3241 aagttcaccg acccggccac gcgggttttc atcagctcgg gaaaccagcc gacgagcacc
     3301 ggcagggcga aataataaac cacaaaaaat accgtggcgg gaataatgaa gcggagcttg
     3361 gaagcgacga gcttcttgaa atcagggtcg ctggcgatct cgttccaatt caccgctggt
     3421 gcggaagacg ctggagctga gct
//
LOCUS       DATDAY010000278         2040 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_199423, whole genome shotgun sequence.
ACCESSION   DATDAY010000278 DATDAY010000000
VERSION     DATDAY010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2040)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2040)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2040
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_199423"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1141
                     /locus_tag="VIS74_05575"
     CDS             <1..1141
                     /locus_tag="VIS74_05575"
                     /inference="COORDINATES: protein motif:HMM:NF018983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016977 - chitosanase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 75 protein"
                     /protein_id="HEY5792748.1"
                     /translation="LAEAGGATAAVDRSKPDSYVLEMRLTIRVPKASQTLEEIGANTP
                     KLPHLLYGLPKMLETAQVSRAYETLYANKLAWNRARLTQLDELISRHNFFDCDTVLEL
                     EHPGTGRKALLGQGDMDVNTDGSDGDRNVTVDGSSPFFQPQTSYRWPKASERANPFLP
                     KAESRLRELKTESGKAPQIENLQNQIRELKRFSFLVSDVDPFIVLPGFMLRDSKGPFA
                     PKLGDYAIVIYENHLYPAILGDVGPSFKWGEASMRLCRELDAKASANRRPVSSLQVTY
                     LVFPGSADAAASPPDLNRWHEHCASLLNEIGGSPVELHRWTDLVKPWPTPTPTPTPAP
                     SPLPSPEASPAISPSATPAVQPAPPAPSPPPAPQELLATPPPAAN"
     gene            1151..1966
                     /gene="kdsA"
                     /locus_tag="VIS74_05580"
     CDS             1151..1966
                     /gene="kdsA"
                     /locus_tag="VIS74_05580"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF003543.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HEY5792749.1"
                     /translation="MSKSAFRIGNLQPGKNFVYILGPCVIESKRFLNEMAREIKAICE
                     GEGVQFIFKASYDKANRTSLKSFRGTGVEDGCRMLAEAGAAVGAPVTTDIHTEAEAEI
                     AAKYVDLLQIPAFLCRQTDLILACAATGRAVNVKKGQFLAPWDIRPIAEKLLAGGCPN
                     FFFTERGTTFGYNNLVADMRSLYWMREQGYPVIFDATHSVQRPGGAGDSTSGDGQLAP
                     VLARAAVAAGCDGIFIETHKNPAKALSDGPNQIPLKQLAKTLRQLEAIRRALA"
BASE COUNT          396 a          724 c          573 g          347 t
ORIGIN      
        1 cctcgccgaa gcgggcggcg ccacggccgc ggtggaccgg tccaagccgg acagctacgt
       61 cctcgaaatg cggctgacca tccgcgtccc gaaagccagc cagacgctgg aggaaatcgg
      121 cgcgaatacc ccgaagctgc cgcacctgct ttacggactg ccgaaaatgc tcgaaaccgc
      181 gcaggtttcc cgcgcctacg aaaccctgta tgccaacaaa ctcgcctgga accgcgcccg
      241 gcttacgcag ctcgatgagt tgatcagccg ccacaatttc ttcgactgcg acacggtcct
      301 cgaactcgaa catcccggca ccgggcgcaa ggccctgctc ggccagggcg acatggacgt
      361 gaacaccgac ggctccgacg gcgaccgcaa cgtgacggtg gacggctcct cgccgttctt
      421 tcaaccgcag accagctacc gctggcccaa ggccagcgaa cgggccaacc ccttcctgcc
      481 caaggccgag agccgcctgc gcgaattgaa aacggaatcc ggaaaggcgc ctcagatcga
      541 aaacctgcaa aatcagattc gcgaactcaa acgcttcagc ttcctggttt ccgacgtgga
      601 tcccttcatt gtcctgcccg gattcatgct gcgcgattcc aaggggccct tcgcgcccaa
      661 gctgggcgat tacgcgatcg tcatttacga aaatcacctg tatcccgcca ttctcggcga
      721 cgtggggcct tcgttcaaat ggggcgaagc ctccatgcgg ctctgccggg aactcgatgc
      781 caaagcctcc gccaaccgcc gtccggtaag ttcgctgcag gtcacgtatc tggtttttcc
      841 cggctccgcc gatgcggccg cctccccgcc cgatctcaac cggtggcacg aacattgcgc
      901 gtccctgctg aacgaaatcg gcggttcgcc ggtcgagttg catcgctgga ccgacctcgt
      961 caagccctgg cccaccccga cccccactcc cactccggcc ccaagcccgc tgccttcgcc
     1021 ggaggccagc ccggccatca gcccgtccgc aacccccgcc gttcaacccg ctccgccggc
     1081 gccaagccct ccgcccgctc cgcaagaatt gctggccact cctccgccag ctgctaatta
     1141 aaaaagggcc atgtccaaat ccgcttttcg catcgggaac cttcagcccg gcaaaaactt
     1201 cgtttacatt ctcggtccct gcgtcatcga aagtaaacgc ttcctcaatg aaatggcccg
     1261 ggaaatcaaa gccatctgcg agggcgaagg cgtccagttt attttcaagg cttcctacga
     1321 caaggccaac cgcacctcgc tgaagtcgtt ccgcggaacg ggggtggagg acggctgccg
     1381 gatgctggcc gaggccggag cggcggtcgg cgcgccggtc acgacggaca ttcacacgga
     1441 agcggaggcg gaaatcgccg ccaaatacgt ggatttattg caaatcccgg ccttcctgtg
     1501 ccggcagacc gatctgatcc tcgcctgcgc cgccaccggg cgcgcggtga acgtgaagaa
     1561 agggcaattt ctcgccccgt gggacattcg cccgatcgcc gaaaaactcc tggccggcgg
     1621 ctgcccgaat ttcttcttca ccgaacgcgg cacgacgttc ggttacaaca acctggtcgc
     1681 cgacatgcgt tcgctctact ggatgcgcga gcaggggtat ccggtcatct tcgacgcgac
     1741 gcattccgtg cagcgtcccg gcggcgccgg ggactcgacc agcggggatg gccaactcgc
     1801 cccggtcctc gcccgggcgg ccgtcgcggc gggatgcgat gggatattta ttgaaactca
     1861 caagaatccg gcgaaagctt tgtcggatgg gcccaatcag ataccgctca aacaactcgc
     1921 gaagaccctt cgccaactgg aggccatccg ccgtgccttg gcttaaattc gcgattctgg
     1981 ccctggccgc cacgctggtg gtcgcccagg gccaggaggc cgctccgtcc cccacgcccg
//
LOCUS       DATDAY010000279         3946 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_1396213, whole genome shotgun sequence.
ACCESSION   DATDAY010000279 DATDAY010000000
VERSION     DATDAY010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 3946)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3946)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3946
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1396213"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..323
                     /locus_tag="VIS74_05585"
     CDS             <1..323
                     /locus_tag="VIS74_05585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792750.1"
                     /translation="WIACPLTFYCICLTLVVFRSPDLERAGVTLHSVILFAGKGPEEV
                     GAWKLWVVLGLAVIHWINFRGILSHWWRRGPDSVFAVAYGCVAATVLLFIPQHYTPFI
                     YFQF"
     gene            484..1512
                     /locus_tag="VIS74_05590"
     CDS             484..1512
                     /locus_tag="VIS74_05590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792751.1"
                     /translation="MVTTPFWRGLASVAIAALFFVATSWLLQVLLPPAIPQGVEEKLR
                     FFAKHKDEFDTLIFGSSRIYYAISPEIFDKTTGENGIQTRTFNFGVNGMFPPETFYLL
                     EQVLKTKPRNLKWVVIEMEEIQNKWKEHEIGTQRILYWHDWPRTALTLQKTLDPRGNA
                     ASSRKVARLWLARRQLATNIALFAKQFVNVGRGAALASSWGEYSSYQEEFEIGPKRDG
                     YRLGGAAMSAERAASFQKILAQQVSDAQPRIIDPATDQAFRDSAALIRKVGANPVFVV
                     TPIILQSPVSFRESPPAPLLVFNDSRKYPALFDTRVRIDEAHLTPEGSEEFTRLLARE
                     FARQTRQP"
     gene            1519..2925
                     /locus_tag="VIS74_05595"
     CDS             1519..2925
                     /locus_tag="VIS74_05595"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HEY5792752.1"
                     /translation="MLFVEFRFFWFFLAVFAVYWSLRRNDSRKIWLLICSYVFYAAWN
                     WKFLFLLAGSSLLDYVVGVMLARTENPRARRGWLILSLGANLGTIAFFKYYNFFVTSA
                     AELLTWLGLPASPHTLNIIIPVGVSFYTFHSMSYTIDVYRGKMRAVSSLLDVACFIGF
                     FPQMVAGPIVRAAAFLPQLRSLQKFSDIDVRPALVLFLTGFIKKACIADAVAPFVDRY
                     FAAPGNFTATSAWVAVLFYAIQIYCDFSGYTDMTIACARLLGYELPVNFRFPYFARNI
                     SDFWHRWHISLSSWLRDYLYIPLGGNRGPRWFVHRNIMITMLLGGLWHGGAWTFVIWG
                     GLHGIALVVNREWQRLTEERPQVRAITRWIAWPLTIYWVCVAWIFFRAVDLPHALPAL
                     ESFVLFRSPGTEDLGAWRLWLVAALGLVHWLNSLGRFSEWWRRLPTPVFAAGYGCVAV
                     LVLLFIPPHYTPFIYFQF"
     gene            2954..3718
                     /gene="tpiA"
                     /locus_tag="VIS74_05600"
     CDS             2954..3718
                     /gene="tpiA"
                     /locus_tag="VIS74_05600"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002144919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEY5792753.1"
                     /translation="MRKKIIAANWKMNMTQGEAADFVATFLLEIGESTEVEIVLVPPF
                     TSLAKVTEALGAAQNIKVGAQNMHWERNGAFTGEISSAMLRDLFVRFVVLGHSERRTL
                     FGETDEIVNRKVRAAHEATLRPIVCVGETLEERDKGSVEEVLSRQVRGSLAGLETKEL
                     GDTVVAYEPVWAIGTGRTATAEQAQAAHAFIRKTLQEISDEATADKIRIQYGGSVKPD
                     NARTLMSQPDIDGALVGGASLDPRSFAQIVQGARDE"
     gene            complement(3731..>3946)
                     /locus_tag="VIS74_05605"
     CDS             complement(3731..>3946)
                     /locus_tag="VIS74_05605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792754.1"
                     /translation="ELRNASGTLLDSNNDWGTSPNQAEIQASGNAPTNPKESAVIQTL
                     SAAPYTAIVRGVGNTSGVGSVEVYQLP"
BASE COUNT          685 a         1199 c         1198 g          864 t
ORIGIN      
        1 cgtggattgc atgtccactg acgttttatt gtatctgcct caccctggtt gtttttcgct
       61 cgcctgacct ggaacgggcc ggggtcacgc tccacagcgt catcttgttt gccggcaagg
      121 gcccggaaga ggtgggcgcc tggaagttgt gggtggtgct cgggttggcc gtgatccact
      181 ggatcaactt tcgcggaatc ctctcccact ggtggcggcg cgggccggac tcggtcttcg
      241 cggtggccta tggctgcgtc gctgcgactg tgctcctgtt cattccgcag cactacacgc
      301 ccttcatcta tttccagttc tgacggccca gccggaggag tcgaaagagc gctggcgcag
      361 cctccgactt gtgacgcccg ctcccgtgtg catgaatagt cgcacgatgg cgaaaaatgg
      421 ccccgagcgc gttcggggcg aaaataaaca tagggcggcg gggcgcacga cccgccaggg
      481 tgcatggtaa caacgccgtt ttggcgcggg ctcgctagcg tcgccatcgc ggcgctcttc
      541 tttgttgcga cgagctggct tttgcaggtg cttctgcccc cggccattcc gcaaggcgtc
      601 gaggagaaac tgaggttctt cgcgaagcac aaggacgagt tcgatacgct cattttcggt
      661 tcgagccgca tctactacgc gatctcgccg gagattttcg acaagacgac gggcgagaac
      721 gggattcaga cccggacttt caacttcggc gtcaatggga tgtttccccc ggagaccttt
      781 tatcttctcg agcaagtcct gaagacgaag ccgcgaaatt tgaaatgggt cgtcatcgaa
      841 atggaggaaa tccaaaataa gtggaaagag cacgagatcg gcacgcaacg catcctttac
      901 tggcacgact ggcctcgcac cgcgctgacg cttcagaaaa cgctggatcc gcgaggcaat
      961 gccgcgtcgt ccaggaaagt ggcgcggctt tggctggcgc gccgccagct cgctacgaac
     1021 atcgcccttt tcgccaagca gttcgttaat gtcggtcgcg gcgctgctct ggcttcctcg
     1081 tggggagagt atagctctta ccaggaagag ttcgaaatcg gcccgaaacg ggacggctac
     1141 cggctcggcg gcgccgcgat gtcggccgag cgcgccgcga gtttccaaaa aatcctggcg
     1201 cagcaagtct ccgacgcgca acccaggatt atcgatcccg ccacggacca ggcctttcgt
     1261 gattccgcgg cgctgattcg gaaagtcggc gccaaccccg tctttgtcgt tacacccatc
     1321 atcttgcaat cacccgtcag ctttcgggaa tcgccgcccg ctccgttgct tgtcttcaac
     1381 gactccagga agtaccccgc gttattcgat accagggttc ggatcgacga agcgcatctc
     1441 accccggaag gctctgagga gttcacccgc ctgcttgcgc gagagtttgc ccggcaaacc
     1501 cgccaaccgt aagaacgcat gcttttcgtc gagttccgct tcttctggtt tttccttgcg
     1561 gtcttcgcgg tctactggtc gctgcgccgg aacgattccc gcaaaatctg gctgctgatt
     1621 tgcagctacg ttttttacgc cgcctggaat tggaaattcc tcttcctgct cgcggggtcg
     1681 agtctgctcg attacgtcgt tggcgtcatg ctggcgcgga cggaaaatcc gcgagcccgg
     1741 cgcgggtggt tgatcctgag ccttggcgcg aatctcggca cgattgcgtt cttcaagtac
     1801 tacaacttct tcgtcacttc ggcggccgag ctgctgacct ggctcggtct cccggcgagt
     1861 ccgcacacgc tgaacatcat tatcccggtg ggcgtgagtt tttacacgtt ccactcgatg
     1921 agctacacga tcgacgtcta ccgcgggaag atgcgcgccg tctcgagtct tctcgatgtc
     1981 gcgtgcttca tcggtttctt tccccagatg gtggcgggcc cgatcgttcg ggcggcggcg
     2041 tttctcccgc agctccgatc gctccaaaaa ttctccgaca tcgatgtccg gcccgcgctc
     2101 gttttgtttc tgaccgggtt catcaagaag gcctgcatcg ccgatgcggt ggcgccgttc
     2161 gtcgatcgct attttgccgc tcccgggaac ttcaccgcga cgagcgcctg ggtcgccgtg
     2221 ctcttttacg ccatccagat ctactgcgat ttctccggtt acaccgacat gacaattgcc
     2281 tgcgcccggc tgcttggtta cgaactgccg gtcaatttcc gcttccccta cttcgcccgg
     2341 aacatctccg atttctggca ccgctggcac atcagcctct cgagctggtt gcgcgattat
     2401 ctctacattc cgctgggcgg aaatcgcggg ccgcgctggt ttgtccatcg caacatcatg
     2461 atcacgatgc tcctgggcgg cttgtggcat ggcggcgcgt ggacgttcgt catctggggc
     2521 gggctgcatg gcatcgcgct ggtggtgaac cgggaatggc agcgcctgac cgaagagcga
     2581 ccccaggtgc gggccatcac gcgctggatc gcctggccgc tgaccattta ttgggtctgt
     2641 gtcgcatgga tatttttccg tgcggtcgac ttgccgcacg cgcttccggc cttggaaagc
     2701 ttcgtccttt ttcggagtcc gggcacggag gatctcggcg cctggaggct ctggctcgtg
     2761 gccgccctcg gcctggtcca ttggctaaac tcgctcgggc gattttcgga gtggtggcgg
     2821 cgccttccca ctccggtttt cgccgccgga tacggttgcg tcgcagtgct ggtgctctta
     2881 tttattccgc ctcactacac gccgtttatt tattttcagt tctgagcaaa ccagcgttcc
     2941 tatctttatg gccatccgca aaaaaattat cgccgcgaat tggaaaatga acatgaccca
     3001 gggcgaagcg gccgacttcg tcgcgacctt cctcctggag atcggcgaat cgaccgaggt
     3061 ggaaatcgtc ctcgttccgc cgttcacctc cctcgccaaa gtcacggagg cgctgggggc
     3121 ggcgcaaaac atcaaggtcg gcgcgcaaaa catgcattgg gaacgaaacg gcgcgttcac
     3181 gggggaaatc agctccgcga tgttgcgcga tctgtttgtc cgcttcgtcg tgctcgggca
     3241 cagcgagcgg cgaaccttgt ttggcgagac ggatgagatc gtgaaccgga aggtgcgggc
     3301 cgcgcatgag gctacgttgc gtccgatcgt ttgtgtcggc gaaacgctgg aggagcggga
     3361 caagggcagc gtggaggagg ttctgtcacg ccaggtccgc ggcagcctcg cgggtctcga
     3421 gacgaaggaa ctgggagata cggtggtcgc ctacgagccg gtctgggcga tcgggacggg
     3481 tcgcaccgca acggcggaac aagcgcaggc cgcgcacgcc ttcatccgga aaaccttgca
     3541 ggagatttcc gatgaagcga ccgccgacaa aatccgaatc caatatggcg ggagcgttaa
     3601 gcccgacaac gcgcgcacac tcatgagcca gcccgacatc gatggcgcgc tcgtcggcgg
     3661 cgccagcctc gatccgcgca gcttcgccca gatcgtccag ggcgcgcggg acgagtaacg
     3721 tcggtctgcc ttacggcagc tgatagactt ccacggagcc gacgcccgag gtgttgccca
     3781 cgccgcgcac gatggcggta taaggagcgg ccgagagggt ctggatcacg gccgactctt
     3841 tcgggttcgt cggagcgttg ccgctggcct gaatttcagc ctggtttgga ctcgttcccc
     3901 agtcgttgtt tgaatcgagg agcgtcccac tggcgttgcg aagctc
//
LOCUS       DATDAY010000280         3970 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_1327273, whole genome shotgun sequence.
ACCESSION   DATDAY010000280 DATDAY010000000
VERSION     DATDAY010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 3970)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3970)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3970
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1327273"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1088)
                     /locus_tag="VIS74_05610"
     CDS             complement(<1..1088)
                     /locus_tag="VIS74_05610"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY5792755.1"
                     /translation="MPTPSDRPSHSDPSALLQAQKMEMVGRMASSVIHDLNNYLTVIQ
                     LNAALLEDVGGDPGERARMLGEISRACMTAADLTRRMLSFARRRESRVEPVHLQPLLD
                     ELIGFLQCMLPRRAKIEADYCEAAAWIQGDRFALNQALMNLVLNALDAGSVEGVRLGL
                     RLENREVEITVTDFGTGIRPEDLPRIFEPFFTTKSAEKGTGLGLPIVQRVAEEHGGRV
                     EVQSECGQGATFRLFFPRLAEGERAPAETGPPPAPRRNGNGILVVEDDPGVRRIAGQM
                     LKRSGYEVVEAADAAEAREAWESHPGKFALLFTDVVLPGTVSGEDLADELRRQEAGLR
                     TLFTSGNAGALRKRDEPFVAKPYPPEVLL"
     gene            1118..1558
                     /locus_tag="VIS74_05615"
     CDS             1118..1558
                     /locus_tag="VIS74_05615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792756.1"
                     /translation="MQALDGIYAGLNAIWNTEFPKPCPRCGRVYESIQEYLSATAAPS
                     NSTGLMDLEAGDSERQVGLFRNCSCGSTLMAFCRDRRDLSEAGQRRRELFGQLLQQLA
                     EAGLPGETARLQLLRALKTGKLAQLEETFRHYSQSEKIPRAPLD"
     gene            1596..1669
                     /locus_tag="VIS74_05620"
     tRNA            1596..1669
                     /locus_tag="VIS74_05620"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1630..1632,aa:Arg,seq:acg)
     gene            1793..2371
                     /locus_tag="VIS74_05625"
     CDS             1793..2371
                     /locus_tag="VIS74_05625"
                     /inference="COORDINATES: protein motif:HMM:NF025852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY5792757.1"
                     /translation="MLDSMRFRERNNKNDLTALFINLRKNGGGIMKCTTLPFAFTFII
                     GLAGLPAPSSAADPETNDHKPVIVRPADLQWSDSPALPAGVKISILYGDMKAAEPIGF
                     RLKLPAGGILAPHTHPVHERVTVISGAFAMGEGEKFDKEALKDMPAGSAAIFPRGCPM
                     FGFAKEETIIQVNAEGPWGINYVNPADDPRKK"
     gene            complement(2640..2903)
                     /locus_tag="VIS74_05630"
     CDS             complement(2640..2903)
                     /locus_tag="VIS74_05630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792758.1"
                     /translation="MIPIVSPSEVTGWRLDPFDLAYNKLEAGREKDLQYVGEMVRSNI
                     TPEFSLGKFPRQNAPNQEVLQNLEANLRQAKEAVRKILQWRAK"
     gene            complement(2913..3209)
                     /locus_tag="VIS74_05635"
     CDS             complement(2913..3209)
                     /locus_tag="VIS74_05635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792759.1"
                     /translation="MKALTRSDLFLALDKCSALTGNREFVVVGSISIPGSKPAAPRGL
                     TIAADIDLFLRFGESGEKNELIDKNFGQGSPFELENNFYIEGVGSWTMMTSPVG"
     gene            3214..3447
                     /locus_tag="VIS74_05640"
     CDS             3214..3447
                     /locus_tag="VIS74_05640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792760.1"
                     /translation="MEPFRLGEKRRLDEGHRRTESLSFLPLGFQQARNFLCGAVFGQS
                     AHSCLHAAKCELQLGRSSHRSQEVPIGNGFDGL"
     gene            3602..3880
                     /locus_tag="VIS74_05645"
     CDS             3602..3880
                     /locus_tag="VIS74_05645"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY5792761.1"
                     /translation="MKAKTVRDLAAIARERRRHLGLSQSQLAKKAGVGRDWVVQFEKG
                     KVTVEWGLVVRALRELGLSLELRVEESQRGVGDLDRILASAQNERRKS"
BASE COUNT          857 a         1162 c         1136 g          815 t
ORIGIN      
        1 agcagcactt ccggcgggta tggtttggcc acaaagggtt cgtcccgctt gcgcagcgcg
       61 ccggcgttgc cgctcgtaaa aagcgtccgg agcccggcct cctggcgccg caactcgtcg
      121 gcgaggtctt cgccggaaac cgtgccgggc agcaccacgt ccgtaaacag caaggcgaac
      181 ttccccgggt ggctttccca ggcttcccgc gcctcggcgg catccgccgc ttcgaccacc
      241 tcatagccgc tccgcttcag catctggccg gcgatccgcc ggacgcccgg atcgtcctcg
      301 accaccaaaa ttccattccc gttccggcgg ggagccggcg gcgggccggt ttccgccggg
      361 gcgcgctccc cctccgccag ccggggaaaa aacagccgga acgtggctcc ctggccgcat
      421 tcgctttgca cttccacccg gccgccatgc tcctcggcca cccgttgaac gatgggcagc
      481 cccaatccgg tgcccttctc cgcggatttg gtggtgaaaa acggctcgaa aatacgcggc
      541 agatcctccg gacgaatgcc cgtgccaaaa tccgtcacgg tgatttcgac ctcgcgattt
      601 tccaaccgaa ggccgagccg gactccctcc accgacccgg cgtcgagcgc attgaggacc
      661 agattcatca acgcctgatt cagggcgaaa cggtctccct ggatccaggc cgccgcttcg
      721 caataatccg cttcgatctt cgcccggcgc ggcagcatgc actgcaggaa gccgatcagc
      781 tcatccaata aaggctgcaa atgcaccggt tcgacccggg actccctccg ccgggcaaac
      841 gagagcatgc ggcgcgtcaa atccgccgct gtcatgcacg cccggctgat ctcgccaagc
      901 atgcgcgccc gctccccggg atcccccccc acatcttcca gcagggcggc atttaattga
      961 atgacggtca gataattatt taagtcgtga atgaccgagc tggccatccg tccgaccatt
     1021 tccatctttt gcgcctggag cagggcggaa gggtcggaat gagaagggcg gtcactcggc
     1081 gttggcacgg gcctcagttt ctggctatcc tcgcaaaatg caagctctcg acggcatcta
     1141 cgcgggcctg aatgcgatct ggaataccga atttcccaaa ccgtgccccc ggtgcggacg
     1201 ggtttacgaa tccatccagg aatacctttc cgccaccgcc gccccgagca acagcaccgg
     1261 cctcatggac ctcgaagcgg gcgattccga gcggcaggtc gggcttttcc ggaattgttc
     1321 ctgcggctcg accttgatgg ctttctgccg ggaccggcgc gacctcagcg aagccggcca
     1381 gcgccggcgc gagttgtttg gccaattgct ccagcaactc gccgaggcgg gcctgccggg
     1441 agaaacggcc cgccttcagc tgctgcgagc cttgaaaacg ggcaaactgg cgcaactgga
     1501 agaaacgttc cgccattact cgcaatccga aaaaatcccg cgggcgccgc ttgactaagc
     1561 ggggacatcg gttattctaa gcagctcttc aaaacgcacc cgtagctcaa ttggatagag
     1621 cgtctgacta cggatcagaa ggtttggggt tcaaatccct acgggtgcac ttgatcgtcc
     1681 tttttcatgg attggatcag gccgggcggc gccggccatt aaaatcatcc cgttacgggg
     1741 ttaaactttc tggcaagata attggctgga ttatctcaat aaataagtcc gattgctgga
     1801 ctcaatgcga ttcagagaaa ggaataataa gaacgattta actgctctat tcataaacct
     1861 tcgtaaaaac gggggaggca tcatgaaatg caccacgctg ccattcgcgt tcacatttat
     1921 catcgggctc gccggtttgc ctgcgccatc cagcgctgcc gatccagaaa ccaatgatca
     1981 caagccggtc atcgtccggc ccgcggattt gcaatggtcc gacagtccgg ctttgcctgc
     2041 gggagtgaag ataagcattc tgtatgggga catgaaggcc gctgaaccca tcggattccg
     2101 actcaaactt cccgccggcg gcattctcgc accccacacg caccccgtgc atgaaagggt
     2161 gaccgtgatt tctggcgcgt ttgcgatggg cgagggcgaa aaatttgaca aggaagcgct
     2221 taaggatatg ccggccggca gcgcggcaat tttcccgcga ggttgtccga tgttcggctt
     2281 cgccaaggaa gagacgatca tccaagtgaa cgccgaagga ccgtggggca tcaactacgt
     2341 caatcccgcc gacgatccac ggaagaagta agcgggagat agggcaagag cggcagggcg
     2401 gctaatccgg cgggcgggtt ggagaggact gatgttaaag ccattccgta aactcctcaa
     2461 agtggggtgt gccgaaagcc tgcggggtgg ggttttatag tatttctcaa atgagatttc
     2521 cgaaaagcga tttattgtag cgcctctaca acgcgcttgg gatcttggaa gcttctttta
     2581 agaatcaccc taaccgccga tttgcagatg aacgcggaga aggatgcagg aatctgccct
     2641 tattttgccc gccattgcag aatcttcctg acagcttcct tggcttgccg caagttcgct
     2701 tcaagattct gaagaacctc ttggttgggg gcgttctgtc gggggaactt gcccaaggaa
     2761 aactcgggag tgatattgga tcgaaccatt tctccaacgt attggaggtc tttttcccgt
     2821 ccggcctcca gcttgttgta ggccaggtca aacggatcaa ggcgccagcc cgtgacttct
     2881 gaaggagaca caatcgggat catgcgcttc tcctatccga ctggagaagt catcatcgtc
     2941 cacgatccca cgccttcgat atagaaatta ttttcgagtt cgaacgggct accttgaccg
     3001 aaatttttgt cgatcagttc gttcttttcg ccgctctctc cgaaacggag gaagagatca
     3061 atgtcagcag ctattgtcag gccgcgaggc gcagcaggct tggacccggg gatagagatg
     3121 ctcccgacaa caacaaactc gcgatttccc gtcagggctg agcacttgtc gagcgccagg
     3181 aaaagatcac ttctcgtaag cgctttcaac gatatggagc ctttccgcct cggtgaaaaa
     3241 aggcggttgg atgaagggca tcgaagaacg gagtcgctga gcttcctccc cctcggtttc
     3301 cagcaggccc gcaatttcct ctgcggagcg gtgttcggtc aatcagccca ttcctgcctg
     3361 cacgcagcca aatgcgagtt gcaactcgga cgttccagcc atcgctcgca agaagtgcca
     3421 atcggcaatg gcttcgacgg tctctaactg tctctagaaa atttaccgga tccaagacgc
     3481 aagccgttgt tcaactttcc agagatcaag gaaagccggc tatcgacgac attataagat
     3541 gtctgtgata gacgactttt ttatttgtcg gcgaacgacg acatgctaga ttcaatgatt
     3601 aatgaaagcc aaaactgtcc gcgacctcgc agcaattgcg cgggaacgcc gccgccattt
     3661 gggcttgtcc caatcgcagc tggcgaaaaa ggcaggcgtg gggcgggact gggtggtgca
     3721 gttcgagaaa ggaaaggtca cggtggaatg ggggcttgtc gtgcgcgcgc tccgcgagtt
     3781 ggggctctcg ctggagctgc gtgttgaaga gagtcaaaga ggggtggggg atttggatcg
     3841 cattctcgca tcagcgcaaa acgagcggag aaaatcgtga gcgactccct gacggtcgtg
     3901 gccgagggac gggttatggg cgaagccctg cttcgcggtg accggctgag tttccgctac
     3961 gacccggact
//
LOCUS       DATDAY010000281         4377 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_2209850, whole genome shotgun sequence.
ACCESSION   DATDAY010000281 DATDAY010000000
VERSION     DATDAY010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 4377)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4377)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4377
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2209850"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2066
                     /locus_tag="VIS74_05650"
     CDS             <1..2066
                     /locus_tag="VIS74_05650"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEY5792762.1"
                     /translation="ITNHQSPITNHQSPITNHQSPITKHQSPITNATALRAADLCFAP
                     PDTRLASHNSPFTIHASPFNLPCMSSPALLTGLAIAIAGILYGGLYEPVFMLPSFLLL
                     AAAGGWIAAGMMAGSLSLRHPAPLLALALSTYVCVRGFAFNPSTGIAVTILAAAAGLA
                     YLSGAVLANGGGARWIWAGLLLAAALLQMAAGWRQLSQTEAAWTLPWLSEQLRGWYSS
                     RSGDRLHGFFLNANHLAWLLNFAGLTALALAVWARIHPLPKVLLAAAAALAFWGSFQT
                     LSRGGLLALAAGLLTFGAISGSIVLFRVGRLRWPLALVGTALAIGGFLVLNTGLERLP
                     QYSVRFLIAAHEGDYRDKIWPTAWRQFQTAPVFGTGSGTYAAHGRMYREDLHFSDPRF
                     AHNDWLQFLAENGAAGMALLIAALLAHVATAKWDWRRLKAEPRFQEGFPQDTTLGFRI
                     GAFACLAAAAVHSLFDFNLHLPANLLLAAVCLGMTAGNGEPARAGREWLPRGAAGIIP
                     LALAVFLLAHAVRNGPPWIRDLQAQNALQQDRLKTAALLLLPPGTFNAASAGAWQARG
                     DLLKRLGERTPEPQAARQWLAMAAAAFDRARQLDPQERFHELKLAATLLQLGDASAVL
                     PLAARTMEFDPLDAQPYLVAGAAAEMQGQIRAARQFYALASRLPHTGNLAERQLQKLT
                     QEPRP"
     gene            2292..3602
                     /locus_tag="VIS74_05655"
     CDS             2292..3602
                     /locus_tag="VIS74_05655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792763.1"
                     /translation="MQAQEFYTTPTSNQPTAVQQTIGNAGIAQDLGVASSGRSQDAAP
                     STASSPDQAGGGSGGESKSLSFFDLAGGTRNPFKISASIREGYDDNTLTTRTDRKGSF
                     YTNLAAGISYDFGSPRLRLNTNLTGGVAYYYTRPGRKLDLSLIYTLSAVYAASSRLTL
                     GISSTIGWYPQPDVGIAGTPFNNQDGSYFYTSTNVDATYQWTSRFSTTTAYTFRALSY
                     SDQDLNEGQGNISQTLSQSFNFQLWPTTTLVAEYRANAITYFDADLNSFGNYALLGFN
                     HVFNPRSRWNLRGGLEQRFNNNPVDGKDIYVGPFAESIFIYRFTSRSEVAWSTRYGTE
                     QSGLTDVTQRQTFRTGISVNHGFTARLSGTFDLFYAVSYYDQPGVIPDYYQNVFQANL
                     GLNFALNRLLSLSTGYQFTGALSPQQLDQEYYRNIVYLGLNANF"
     gene            3672..>4377
                     /locus_tag="VIS74_05660"
     CDS             3672..>4377
                     /locus_tag="VIS74_05660"
                     /inference="COORDINATES: protein motif:HMM:NF014732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HEY5792764.1"
                     /translation="MHQSPPQEIKLHALDYLRIIRVRWPIVLLVFLLITITAMVVTYF
                     APRKYASTATMQVKAADFFMNLFGSSSSPNAGTAGGPGFANTQNEIIQRTEVLYPVVE
                     SLQLIQRWAPLGVTNKRDAYLRLRSLLQIKAILNTDLIEITATTLSPKESAEIANAVA
                     EAYKNKRIDEVKTWLNRSLSTLEGEVETQRQAVGRLQGDAARIRRQKNITDLNPDTVE
                     EASGTKDRALIEIEQQL"
BASE COUNT          830 a         1457 c         1199 g          891 t
ORIGIN      
        1 caatcaccaa tcaccaatca ccaatcacca atcaccaatc accaatcacc aatcaccaat
       61 caccaatcac caaacaccaa tcaccaatca ccaatgccac tgcgctccgt gcagccgatc
      121 tctgcttcgc tcctcccgac acccggctcg ccagtcacaa ttcaccattc acaattcacg
      181 cttccccttt taatctcccc tgcatgtctt cccccgccct cctgaccggc cttgcgattg
      241 ccattgccgg catcctctat ggcggcctct acgagccggt cttcatgctg ccctcgttcc
      301 tgctgctggc cgccgcggga ggttggattg cggcgggaat gatggcggga agcctttcgc
      361 ttcggcatcc cgcgcccctt ctggcgctgg cattaagcac ctatgtctgc gtccgcggtt
      421 tcgcattcaa tccctccacg ggaatcgcgg tgacgatcct ggccgcggcg gcggggctgg
      481 cttatttgtc gggagcggtg ctggccaatg gcggtggcgc gcgctggatc tgggcggggc
      541 tgttattggc ggcggccctc ctgcaaatgg cggccggctg gcggcaattg agccagacgg
      601 aagccgcctg gactctgccc tggctgagcg agcaattgcg cggctggtat tcgtcccggt
      661 cgggggaccg cctgcacggg ttctttctca atgccaatca cctggcctgg ctgctgaatt
      721 ttgccggatt gaccgcgctg gccctcgcgg tttgggcgcg gattcatccc cttcccaagg
      781 tcctcctggc ggcggccgcg gcactggcgt tctggggaag ttttcaaacc ctgagccgcg
      841 gcgggctgct ggcgctggcg gcggggctgc tgacattcgg cgccatttcc ggaagcatcg
      901 ttttattccg ggtgggacgg ttgcgctggc ccctggccct ggtcggaacc gctctggcaa
      961 tcggcggatt tctggtattg aacactggcc tggagcggct tccgcaatac tcggtgagat
     1021 ttttgatcgc cgcccatgag ggcgactacc gggacaaaat ctggcccacc gcctggcggc
     1081 aatttcagac ggcgccggta tttggcaccg gttccggcac ctacgcggcc catggccgga
     1141 tgtatcggga ggatcttcat ttcagcgatc cgcggttcgc ccacaatgat tggctgcagt
     1201 tcctggcgga aaacggcgcg gcgggaatgg cgctgttgat cgcggcattg ctggcgcatg
     1261 tggcaaccgc gaaatgggat tggcggcggc tcaaggccga gccccggttt caggaggggt
     1321 tcccgcagga taccaccctc ggtttccgga tcggggcatt cgcctgcctc gccgcggcgg
     1381 ccgttcattc cctcttcgat tttaatctgc acctgcccgc aaacttgctg ctggccgccg
     1441 tctgcctggg aatgacggcc gggaatggcg aaccggcccg ggcagggcgg gaatggctcc
     1501 cgcggggggc ggccgggatt attccgctgg cgctggccgt ttttttgctg gcccacgcgg
     1561 ttcggaacgg accgccctgg atccgggatc tgcaggcgca aaacgccctc cagcaagatc
     1621 gcttaaaaac ggccgcgctt ctcttgctcc cccccggcac tttcaatgcc gcctcggccg
     1681 gggcatggca ggcgcgcggc gatctcctca agcgcctggg cgagcggacc cccgaacccc
     1741 aagcggcccg gcaatggctg gcaatggctg ccgccgcgtt cgaccgcgcc cgccagctcg
     1801 atccgcagga acgattccac gagttaaagc tggcggcaac gctgcttcag ctcggggatg
     1861 cttcggccgt ccttccgctg gccgcgcgaa caatggagtt cgatccgctt gatgcgcaac
     1921 cgtatctcgt cgcgggcgcg gccgccgaaa tgcagggaca aattcgagcg gcccggcaat
     1981 tttacgcgct cgccagccgg ctgccgcaca ccggcaacct ggccgagcgg caactgcaaa
     2041 agttaacgca agaaccccgg ccgtaatccc ttatcctccc gaatattttg ccggcaaaaa
     2101 agtgaattgg gccgaaccag ccgaaagcaa agtttgccaa aaaatcatcg cgaaattagt
     2161 aattgtcaga aggtaaggaa tcctctttac tgcgcgcacg catcatgaaa aaagcgtccg
     2221 cgccccttgt acgcccattt ctccccctgg cggtcggctt aatggcggct ggttcctcca
     2281 ttactccctc tttgcaggct caggagttct ataccacccc caccagcaac cagcccacgg
     2341 cggtccagca aaccattgga aacgctggaa ttgcgcagga tttgggagtc gccagcagcg
     2401 ggcgttccca ggacgccgcg ccgagcaccg cttccagccc ggaccaggcg ggcggcggca
     2461 gcggaggaga atccaaatcc ctttcgtttt ttgatctggc gggcggcacg cgcaacccct
     2521 tcaaaatttc cgcatcgatc cgggaaggct acgacgataa caccctgacc acccgcaccg
     2581 accgcaaagg cagtttctat accaaccttg ccgccggcat cagctatgac tttggttctc
     2641 cccggcttcg cctgaacacc aacctgaccg gaggcgtcgc ttattattac acccgtccgg
     2701 gacggaagct ggatctctcg cttatctaca ccctgagcgc ggtttacgcc gcttcctccc
     2761 gcctgacgct cggcatttcc tcgaccatcg gctggtatcc ccagccggat gtcggcatcg
     2821 ccggaacgcc gttcaataat caggatggca gttatttcta cacctccacc aacgtggacg
     2881 ccacttacca gtggacctcc cggttttcca ccaccacagc ctacaccttc cgggcgctca
     2941 gctattcgga tcaggatctc aacgaaggcc agggcaatat ctcccaaacc ctcagccagt
     3001 cctttaactt ccaattgtgg ccgaccacga ctctggtggc cgaataccgg gcgaacgcca
     3061 tcacctactt cgatgccgac ctcaacagct tcggcaacta tgcgctgctt ggctttaacc
     3121 acgtgttcaa tccccgctcc cgctggaatc ttcgcggcgg tctcgaacag cgcttcaaca
     3181 acaatcccgt ggacgggaag gacatctatg tcggcccctt cgccgagagc atcttcatct
     3241 accgcttcac ttcccgttcc gaggttgcct ggtccacccg ctacggcacg gaacaatccg
     3301 gcctgacgga cgtcacccag cggcagacct tccgcacggg catcagcgtc aatcatggct
     3361 tcaccgcacg gctttccggc accttcgacc tgttctacgc ggtcagctat tacgatcaac
     3421 ccggagtgat tcccgactat taccaaaatg tctttcaggc caatcttggg ctgaattttg
     3481 cgctgaatcg cctgctttcc ctttccaccg gctatcagtt caccggcgct ctctcccccc
     3541 aacagctcga ccaggaatac taccggaaca ttgtgtatct gggattgaac gcaaatttct
     3601 aagttcatcc cattgatttc ccggcggcgg aattgctttt ccggggaagg caatgctact
     3661 cttttaaggc catgcatcag tcaccgcccc aggaaatcaa gctccatgcc cttgattacc
     3721 tgcgcatcat tcgggtgcgc tggccgatcg tcctgctggt ctttctgctg attacgatca
     3781 ccgccatggt ggtgacttac tttgcccccc gcaagtatgc ctcgacggcg accatgcaag
     3841 tcaaggcggc ggacttcttc atgaatcttt tcgggagttc ctccagcccg aatgccggca
     3901 ccgcgggcgg gccgggtttc gcgaacactc agaatgagat tattcagcgc acggaggtgc
     3961 tttatcccgt ggtggagtcc ctccaattga ttcaacgctg ggctccgctc ggagtgacca
     4021 acaagcggga cgcctatctg cggctgcgca gcctgctcca gatcaaggcg atcctcaata
     4081 cggatctgat cgaaatcacc gccaccacgc tttcgccgaa ggaatccgcc gaaatcgcca
     4141 acgcggtcgc cgaggcctat aaaaacaagc gcatcgacga ggtgaaaacc tggctgaatc
     4201 gttccctttc caccctggaa ggcgaagtcg aaacccagcg ccaggcagtc ggacgccttc
     4261 agggtgacgc ggcccgaatc cgccggcaga aaaacattac cgatctcaat cccgacaccg
     4321 tggaagaggc gagcgggacc aaggaccgcg ccttgatcga aatcgaacaa cagctga
//
LOCUS       DATDAY010000282         2892 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_2099777, whole genome shotgun sequence.
ACCESSION   DATDAY010000282 DATDAY010000000
VERSION     DATDAY010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2892)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2892)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2892
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2099777"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..342
                     /locus_tag="VIS74_05665"
     CDS             <1..342
                     /locus_tag="VIS74_05665"
                     /inference="COORDINATES: protein motif:HMM:NF015898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HEY5792765.1"
                     /translation="WQIMKLLWRRSPQTAQELVRELAASWSESTVKTLLNRLVRKGAL
                     RHEKEGKAFWYSPAVTEAECRAAESATFLNRVFDGALSPMLAHFVESSSLNDEELAEL
                     ERLVRRKRRGS"
     gene            339..1685
                     /locus_tag="VIS74_05670"
     CDS             339..1685
                     /locus_tag="VIS74_05670"
                     /inference="COORDINATES: protein motif:HMM:NF017390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HEY5792766.1"
                     /translation="MSASGLALLVDLTLRGALATGALLGLDFLVGRWLAARHRRWLWL
                     LAPIAFLLPIWCRLPVLPAAAGPAMKAMAAPGISDEQRPATVRGGAVSWGGVREPAAL
                     LWMAGVLVGTIVVATRTLRASRLWGASRFSTDPKLLDLLEDCRRLAEVRAPIGVIVSD
                     RVASPALLGWLRPKLLLPASWVESASPAQLRDVLLHELAHLRAGDLAWGWLFTAARVL
                     HWFNPLAHVAARRWQAAREDAADEFVLRLRPASADSYDETLLAFLESARRPQPFGALG
                     ISENLLNLKHRIQMIQTHPRRVLPSVVACLLLGLATSLILVQATRAESSEADRKTAAV
                     AAMTAWLQGIDQGGYAQSWSAASKNFRAAVTQEQWVAMSRQVREPLGACTKRELVSVL
                     LQNGVPQPGGKSLPGEFAIAQFKTSFANLFSAVETVTFEREADGVWRASGYYIKPG"
     gene            1837..2376
                     /locus_tag="VIS74_05675"
     CDS             1837..2376
                     /locus_tag="VIS74_05675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792767.1"
                     /translation="MSKSLPFTAALLPALSALVPSLHVQIATSTLLRGETAKGRYDIR
                     LSDDGRELRTSFLAKGRNPLKQSKLLLRSPLSDEGSVQGHAIVIRENEMRLPWIQRAQ
                     DSRLGDDIVTVRPFGSDETAPYVAVRTSALTGFISLTVENAREMSPAEAKFVAKALAK
                     AAKLAAVSKIEPVALSAAA"
     gene            2473..>2892
                     /locus_tag="VIS74_05680"
     CDS             2473..>2892
                     /locus_tag="VIS74_05680"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEY5792768.1"
                     /translation="MKAKRLAVLKTYKLFIGGAFPRSESGRVRPALAADGKTLLGNYA
                     HASRKDFRDAVGKARAAQPKWASASAYLRGQILYRAAELLECRRAELEAELGRATGAA
                     AADEVTAAIDLLVHYAGWTDKYGPLFSAVNPVASPHFN"
BASE COUNT          440 a         1010 c          942 g          500 t
ORIGIN      
        1 tggcagatca tgaagctgct gtggcggcgc tcgccgcaga cggcgcagga gttggtgcgg
       61 gagctggccg cgagttggag tgagtccacg gtcaagacgc tcctcaaccg gctggttcgc
      121 aagggagcgc tccgccacga aaaggaggga aaggcgttct ggtattctcc ggcggtgacg
      181 gaagccgagt gccgcgcggc ggaaagcgcc acgtttctga accgcgtgtt cgacggcgcc
      241 ctcagtccga tgctcgcgca tttcgtcgag tcgagttcgc tcaatgatga ggaacttgcc
      301 gagctggagc ggctcgtgcg gcgcaagcgg aggggatcat gagcgcgtcc gggctggcat
      361 tgctggtcga tctcaccctt cgcggagccc tggcgacggg ggcgctcctc gggctcgatt
      421 tcctcgtcgg ccgatggctt gccgcccggc accggcggtg gctctggctg ctcgcgccga
      481 tcgcgttcct cctcccgatc tggtgccggc tcccggtgct gcccgcggcg gcggggccgg
      541 cgatgaaggc catggccgcg cccggaatct ccgatgaaca acggcccgcg accgtccggg
      601 gcggagcggt ttcctggggc ggggttcgcg agccggcggc gctgctctgg atggcgggcg
      661 tgctggttgg cacgatcgtc gtcgccacgc gcacgctccg cgcctcccgg ctttggggcg
      721 cgagccggtt ctccaccgac ccgaagctcc tggatttgct cgaggactgc cggcgcctgg
      781 cggaagtgcg ggccccgatc ggcgtgatcg tgagcgatcg agtcgcctcg cccgcgctgc
      841 tcggctggtt gcggccgaaa ttgctcctgc ccgcaagctg ggtggaaagc gcctcgcccg
      901 cgcaattacg cgacgtcctg ctgcacgaac tcgcgcacct gcgcgcgggc gatctcgcct
      961 gggggtggct cttcaccgcc gcgcgggtgc tgcattggtt caatccgctg gcccacgtcg
     1021 cggcccggcg ctggcaggcc gcgcgcgagg atgccgccga tgaattcgtg ctccggctgc
     1081 gtccggcctc ggcggattcc tacgacgaga cgctgctggc ctttctcgag tccgcgcggc
     1141 ggccgcagcc cttcggcgcg ctcggcattt ccgaaaatct cctcaacctc aaacaccgca
     1201 tccaaatgat ccagacccat ccccgccgcg tccttccctc cgtcgtcgcc tgcctcctgc
     1261 ttgggttggc gacatcgctc atccttgtgc aagctacccg ggcggaatcc tccgaggccg
     1321 accgcaaaac cgccgccgtc gccgcgatga cggcctggct ccagggcatc gaccagggcg
     1381 gctacgcgca aagctggagc gccgcgtcga aaaatttccg cgcggcggtc acgcaggagc
     1441 agtgggtcgc catgagccgg caggtgcgcg agcccctcgg agcctgcacg aagcgggagc
     1501 tcgtctcggt gctcctgcaa aatggcgtgc cgcagcccgg cgggaaatcg cttcccggcg
     1561 aattcgccat cgcgcagttc aagacctcgt tcgcgaatct cttttcggcc gtggaaacag
     1621 tcaccttcga gcgcgaggcc gacggcgtct ggcgggcgtc gggctattac attaagccgg
     1681 gttaagcgga atactccgcg gacgctggag cgggctcaaa ttcgacgacg tgcatcccgg
     1741 agcccgggcg ctgatcttcc cgattgaaat gtgacgattt tgttacatag tggaggattc
     1801 gtgcggccgg gcatttcctc cgccgcggca atttccatgt caaagtccct gcccttcacc
     1861 gccgccctgc tgcccgcgct cagcgcgctt gttccctcgc tgcacgtcca gatcgccacc
     1921 tccacccttc tgcgcggcga gacggccaag ggccgctatg acattcgcct ctccgacgac
     1981 ggccgcgaac tccgcacttc ctttctcgcg aaaggacgca atcccctcaa gcaaagcaag
     2041 ctgctcctgc gttctcccct cagcgacgag ggttccgttc aaggtcacgc catcgtgatt
     2101 cgtgagaacg agatgcgcct gccgtggatt caacgcgcgc aggactccag gctcggcgac
     2161 gacatcgtca cggtgaggcc gttcggcagc gacgagaccg cgccctatgt ggcggtgcgc
     2221 acgagcgcgt tgaccgggtt catttcactc acggtggaaa atgcccggga aatgagcccc
     2281 gcggaagcga agttcgtcgc caaggcgctg gccaaggccg caaagctcgc ggccgtcagc
     2341 aaaatcgagc cggtcgcgct gtccgcggcg gcttaactgc aaggcccggc gcacccgaag
     2401 gcgccgccgc ccgacggaaa ttgcctcgtt tttgggagtc cggcgcgtat cctgcttttc
     2461 cgttccccgc ctgtgaaagc caaacgtctc gccgtcctca agacctacaa acttttcatc
     2521 gggggcgcct ttccccgcag cgaaagcggc cgggtgcggc cggccctcgc tgccgacggg
     2581 aagacccttc ttggcaacta cgcccacgcc tcgcgcaagg acttccgcga cgccgtgggg
     2641 aaggcccgcg ccgcccagcc gaaatgggcc tccgccagcg cctacctgcg cggacagatt
     2701 ctctaccgcg ccgccgagtt gctcgaatgc cgtcgcgcgg aactcgaggc cgagcttggc
     2761 cgcgccaccg gcgccgccgc tgcggacgaa gtcaccgcgg ccatcgacct gctcgtgcac
     2821 tacgccggct ggaccgacaa atacggcccg ctcttctcgg cggtgaatcc cgtcgcgtcg
     2881 ccccacttca at
//
LOCUS       DATDAY010000283        16678 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_2210349, whole genome shotgun sequence.
ACCESSION   DATDAY010000283 DATDAY010000000
VERSION     DATDAY010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 16678)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16678)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16678
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2210349"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1096
                     /locus_tag="VIS74_05685"
     CDS             <1..1096
                     /locus_tag="VIS74_05685"
                     /inference="COORDINATES: protein motif:HMM:NF014879.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792769.1"
                     /translation="NHILEDGLKAVKRIPLAQARPRTTDFAADYIDDLAKVINLDAIR
                     ASGIKIGVDPLGNSSLAYWPRIVDKYGLNLEVTNPDLDFTFRFMSVDWDGKIRMDCSS
                     PYAMAGLVALKDQFDIAFANDTDADRHGIVTKSGGLMNPNHYLAAAIFYLFQNRPGWS
                     ADAGIGKTLVSSSMIDRVAKKLGRRLVEVPVGFKWFVDGLYTGTLGFGGEESAGASFL
                     RLDGTPWTTDKDGIILGLLAAEILAVTGKDPCEHYQTLTAEFGAPIYERIDAAATPEE
                     KNKLKKLSPSQLKASTLAGDRITATLTEAPGNGAAIGGLKVCTEKGWFAARPSGTEDV
                     YKIYAESFVDPDHLARIQAEAKQIVAGAIA"
     gene            1121..2755
                     /locus_tag="VIS74_05690"
     CDS             1121..2755
                     /locus_tag="VIS74_05690"
                     /inference="COORDINATES: protein motif:HMM:NF023580.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HEY5792770.1"
                     /translation="MRLQINFPASRTSNSFAGKLGVTLFFGLFFGLGSVFFCLAGMDF
                     IRTVQPYFWSPAPARILSGEVVPDGDDHKIHVRFRYSWQGRTFESATFSQPEESFNHY
                     DTAFEALAALPAGTETTCRVNPKAPEQAVLRLRGLWMGFVMLIPLIFVAVGGLGMWGA
                     WARPAPATRRSSQPVKKSRVTGRVTGAALALAGAGLLAAWAVPMWLKAADSTRWPETP
                     CVILSSCVKSHSDSDGTTYSVAIAYRYTFEGREYRSDRFDLMNASSSGRAGKEALVAR
                     YPAGSTRVCYVDPDRPSQALLRRGFTWSLLLGLIPLALLLFGLFLCFQKLGKTNPAAQ
                     APRVDATGLLKSKESPGLRLGGTLAFALFWNGLLSVFLLDLIGGFRRGQPDWFLALFL
                     CPFVLIGLAALGAAGYYLLALGNPRIRLRLETGRIEPGAPLKLAWQMDGAVRRLERLV
                     IYLEGREEATYTRGTSSVTDKRVFFRQPLADVTGTWMDFQEGRGAISIPREAPPSFRA
                     SHNRIVWSCQVWGKIPFWPGLREEFEIPLQPPFPAA"
     gene            2752..3177
                     /locus_tag="VIS74_05695"
     CDS             2752..3177
                     /locus_tag="VIS74_05695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792771.1"
                     /translation="MNEFTATLAPEKTRGRPGDPIRGRASWHGPHPPRSAELVLFWRT
                     EGKGDRDSETVFRLEFDQPQADDDRPFALTAPPFPHTFSGQLISLIWELELLLDDRGA
                     GSVEITLSPFPEEISLERAEWITMDAPGPQFPFPWRKTR"
     gene            3174..3770
                     /locus_tag="VIS74_05700"
     CDS             3174..3770
                     /locus_tag="VIS74_05700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792772.1"
                     /translation="MKARENPFRRERIEALAYRAPDFDWAELEARLTRLGGQGALVGP
                     KGHGKTTLLEQWAARVPGSLHFRIAEGQRFLTPAQRQALCESRAAFVLVDSAEQLGWL
                     GWRELRRRVGNARRLAITTHRPGRLPTLRHCQTSPQLLGELAAELTGVPRDCTALWRR
                     HRGDLRRALRELYDAGHCKKPLPAPRSVASLNAAPIPI"
     gene            3767..4450
                     /locus_tag="VIS74_05705"
     CDS             3767..4450
                     /locus_tag="VIS74_05705"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEY5792773.1"
                     /translation="MSNQRIVFFFDIDNTLLDNDRVTKDIAKKLDTVVGETAQKRYWE
                     IFEQLRKEEGYADYLAALQKFRFEDKFDPKLIYLSLFLTDYPFANRIFPSALDVIDRA
                     NEWGQAVILSDGDVVFQPRKAFRAGLLDAVGGRALIYIHKEEELADAEKRYPADHYVM
                     FDDKLRILEAMKKIWGAKLTTVFVRQGHYAFEPGVEEKYAKADLTIDHIGDALNFTRD
                     QFILAAYHS"
     gene            4447..5316
                     /locus_tag="VIS74_05710"
     CDS             4447..5316
                     /locus_tag="VIS74_05710"
                     /inference="COORDINATES: protein motif:HMM:TIGR03709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2 family protein"
                     /protein_id="HEY5792774.1"
                     /translation="MKSSAYHVKPGSRVRLASHDPGDTGHYKDKHAARTRLAEDILEM
                     QRLQDIFYAADSYSLLIIFQALDAAGKDGAIKHVMSGLNPQGCQVAAFKSPSATELDH
                     DYLWRCNIRLPERGRIGIFNRSYNEDVLAVRVHPEYLAAQKLPPKLTGANLWKRRFEE
                     ISHYEKYLADNGTRILKFFLHVSKDEQKRRFLERIENPDKNWKLSPSDVKERQHWHDY
                     EAAYEDLLSHTSTDWAPWYVIPADKKWFSRTLIANVIVRELQKLKLSYPKVSPAHLAA
                     IQESKAALLAEKD"
     gene            5323..6930
                     /locus_tag="VIS74_05715"
     CDS             5323..6930
                     /locus_tag="VIS74_05715"
                     /inference="COORDINATES: protein motif:HMM:NF037997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015114 - phosphate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi symporter"
                     /protein_id="HEY5792775.1"
                     /translation="MGAGASIANELVLGLGLFFLGMVLTGDNLRLLSGPSFRSLVGKP
                     ARTPALGGLIGLGFGALMQSATAVTFILANMVRARLIELAAAVPILLWCNVGLTSLAF
                     VVTFDIHPLVAWFVGGSGILLGLVKKPGWRATAGVCLGIGLILYGLESMSAGAAPLRN
                     LAWFQAILHLFERDPLFAFAGGFIIAALLQSNTGATMLIITLFQAGAFSSSIASPLIY
                     GANLGAIPLRNLLAFSQGPAARGLARVEDAFCLLSGLLMMALFYLEEFARIPLVHALA
                     AAISPSPNTQLALVFLFSNLLPAALLSPLGGRIARFIQQRFPPPSEELPAQSFLNSKA
                     LDDPSTALDLFSKEIIRLLKTVQPQPIESPEGEDEEGQPDTAFSEIAQKIDQFGIDLS
                     RKSMFSRKDAHRLHLLRAEFSMVRYIEETVRAFNRVLGQEEYRKRMETEATAMRQFLE
                     KTLAWAGQAADSLAEPDIQALRETTRKTDPDYDRVRQLGYRRRNTESAENNLWLASLQ
                     DDFEMALWMLHRFSKNLTRRAEASAAR"
     gene            6992..8806
                     /locus_tag="VIS74_05720"
     CDS             6992..8806
                     /locus_tag="VIS74_05720"
                     /EC_number="1.5.1.20"
                     /EC_number="2.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF006396.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional homocysteine
                     S-methyltransferase/methylenetetrahydrofolate reductase"
                     /protein_id="HEY5792776.1"
                     /translation="MNFLEELGKRVIGGDGAMGTLLMERGIALNQCFEEICLSNPELV
                     RGVHRDYAAAGAELIETNSFGANAVRLARHGLQKCVGALNRQAAQIARQAVHGAPAWV
                     AGSVGPLGLTPAQAEEQGIDRAAVFREQIAALIEGGVDVLLFETFQSLDELRIALRAK
                     AELSDCPAIGCLTLSEGGRLADGATLTEAFRQLADAQASVVGINCVSGPQAAIRLLEN
                     VESPLPLAVYPNAGRPRLYEGRYVYDSSPEYFAESAQRLAELGANLVGGCCGTTPAHI
                     AAISRQLKSFGPPVKPRKKIAVVEREKIKLAVAGDSILDRLKAGKTVIVTELDPPKTL
                     TLDRYFEAAEKLTEAGSDAITLADNSLAILRVSNLAIGAMLKARGIEPLLHLSCRDKN
                     VLGLQSELMGAAAMGIRHILPLTGDPAKVGDHPGATSVYDVNSIGLLEIARRMNEGYT
                     QAGTDLKKLPGFVMGCTFNPNAKNLDAQISRLQRKINAGAQYVLTQPVFDAGLVRLAA
                     EKVRHFNIPIFFGVWPILNGRQAKFLHNEVPGIRIPEPVLAEMEGLEGAEGRRHGIAI
                     AKEVCRAALDEFPCVYLITPFQVYETTAELAEFVRGRT"
     gene            complement(8803..16092)
                     /locus_tag="VIS74_05725"
     CDS             complement(8803..16092)
                     /locus_tag="VIS74_05725"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY5792777.1"
                     /translation="MRSLSLAWLLLLFPIAGIRAEKLSPEVLARYEEILIKNPNPGPA
                     FDKVYLYYLEAGNLAELSDKWAKLAAGRDANAASYELLLGLLEERRGNGESAARHFET
                     HLAASPASAPAWTALARLAARKPDLAKAAEAYRRAIPLTSNTEDQLGLYRELALLYER
                     NFQADQAIQVWKEVAAAYPDDPVVMEEAASAFAEAGKQEEARQLLDGFLKNSSPGPAA
                     KLRLEMLAASLLEKSGKRAEALAEYEKLLEDVSPKSWLQRELRSRIETSFRQADDLPG
                     LAQYYETRLKTHPNEVESAMRLSNVDAELGKSADALNYIRQASEWAPEDMEIKLVLAS
                     RLTEAKQEAEAEKRVGQWIQQNPERKELVETLARIQWAHFKRTGEAGIQAQVVETLKS
                     LVPADKPMAADYSKCADLLSGYEFDETAAEFYEKALAMDPQSSDTIERLGRSRLKAKQ
                     PEKAWGVLEGLTAKPNAEASDYVRLARLQEPNGKVNEAIQTAAKGIEKYPDSLDLLNL
                     SYQLLTREKRMEEALAMLDQLIARVPSNAALVQAETQKVSLLQNLKKAEGEEGELRAR
                     LENGETLSEPEFRLLLRLQSALDRKPEAALAAAEKTFPDSLPLLQLRMEVARKSGNTG
                     QTIALLKKLAERDSEQRQGWLQEVANSYQKANQPDQALQAARDVIAAFPAKPDGYLFA
                     GNLALQMGKFDEAAGWYTQAAGLSEKPRDPLSRLARLYFQRGNYSDAIRVYEEAFEAE
                     SEPEGRVAILGEMVNPYLLSNRIDELIQRFKKRQEGESEDGWRYALYLSQLYLRLNDY
                     PSAREQLIRVYAARPNDARMVRQIIRISEQENNPKERSKYWKRLLELEPTPANQLQFS
                     QILFAEDRSGEALQLLEQNAAEWTGKPEVFYGLVSAIKKPAAAKAALELWNRKTGRSE
                     VDAFEFAMNLLLESSVLPRKEFDEKLWSIVANPPPVLIPPPSKNQSPVVFYLNSMMAL
                     SQSSQNEWLQRRIFLTSLTLQLRQLVMSRKSGGGSFVSFSRQQPLPSPSEKISVRDYA
                     LAWAVATTAESAAEAEVASRAQAALASSDAPPEEKLFVQSLLGMNEPFFKLVEDYVNN
                     RPANPELDFYCLSVLASGKPVEGVENALLKQFTERVAARNPKDFFSAFTLASACQKLG
                     DMEKARAIFKPALGEIDLQANPQMNIAFAAYGALKFDAVPAFKDFLARVRANPNLQSP
                     SPYMDRLAYLLSWTYQFQENNESLPDSKQMLALTRVLLEDYFNRKLSPISRAQMNQGF
                     FGFGGSNLIVPTKYFDASDLAALNTVFERARAAGVAGEMMEMVNQLEPQVPANQKVAV
                     RLLATVMLSWVGKKEESAAAARKLLEETSDADIRLVYAKILAQAGKNPEAIAILQEIK
                     PIDGQTYRCAQFDMLAASKITGDKALSEKMVAELSQARLSPQERQILSNYTGQPLRSP
                     ASGGSTSANPGISLNEQVNQLYQLAREEKTDEAMKLADQIFANDLVSKYKPDEWNPAE
                     AACGALNRMSRLIGQIRAWEKQLEAAPDSQRLLFLIATGYGITGAADTSHYTKDGFSS
                     PLWLKIQREGEVFRGSLSRDGRQWEVFKETRLNLPAKTYIGLAVTAHTDAKLATAEFQ
                     DVTWKGAVEPAEGVSPETGWAARDIGRPKIAGKEVLQDGKIWVSASGEDIFRRKDRFH
                     YVYRTLTGDGEMIARVNSLDRTSEWSKAGVMIREGLGQNDANIMMTWLAEGSASLQWR
                     QTRDKALDYWQRLAELRNQDPIFNTQIAKALRTQGRDEEALAVYRDVIKRDPEMIVDE
                     PNEMIQLFEDAGKLDELITIEFPPANPQGRSVDVGWYFECLSDLLIRKERKDEAVRAL
                     QQGLKLSQEGRDAYRRGSLTLKLIEQYLAQERKDEAKKLLLEMFEASSGSRAPTRRQS
                     NSLLGSAASFGSYGDFDPFFSSIGSSDDGLPSSDAQRYLELAKTQGWLKEAREIVAAS
                     AKADVSEKNRFLALALRAMERDASVVLEVAQLGREPFDSAKAGDFQTQGAIALCLSQL
                     LRWPATQSAALDWIQRIIDSPDDAQGNTPFLKNALAPWKFRLDAVRGILSVPPEKLLG
                     NFIVKLSGPQGQYNAPYGIILPCWNGLIAKENYAECERLLATVSGLPANFRGRFDMKK
                     LARMREELKFYKEGGNAVAILWLTLKEGRLELRWAFKPVDESKLTPAFPGRRASSNDG
                     KYTLKILAGPNPKELKEAADLPAVKSMGKWSEKLPAETRFVRADLVAADGKITPGPVI
                     PIVPPSGNLLKNPTFKELPKDLAANDPKKTGWNCGSDGWWLKCPNSLTSNGSSMLFAT
                     TSRGRDQAEIVANRVKIDPTQNYLLAARFGSPGGAASGAIGVRFFDEAGNSVDNVSVG
                     ASATQGCQQVYQLFAPGEGARTINAKARFAEVYFRISGLSPVGDVYFGSPPAEAKINE
                     PAKPKEG"
     gene            complement(16101..>16678)
                     /locus_tag="VIS74_05730"
     CDS             complement(16101..>16678)
                     /locus_tag="VIS74_05730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792778.1"
                     /translation="EFSRFWAQTIRRLMRSDSLKRLPAELRPDKDGLKLTVDAVGPRG
                     EFLNDTEGEAVVITPSNESLKLPLERTAPGRLEARWPAPRRGAYHVQFQLKSKGEPIA
                     SQYESFVRGYPNEFLPQLPNTELLRELSERTRGVYDPAPEQIYKLPPQSTAAERELWP
                     WLVALALLLFIADVGVRRLSAISRFNPQILS"
BASE COUNT         3165 a         5475 c         4750 g         3288 t
ORIGIN      
        1 gaatcacatc ctcgaagacg gcctcaaggc ggtcaaacgc atcccgctcg cccaagcccg
       61 cccgcgcacg accgacttcg cggcggacta cattgacgat ctcgcgaagg tcatcaatct
      121 cgacgccatc cgcgcctccg gcatcaagat cggcgtggat ccgctcggca attcgtcgct
      181 ggcctactgg ccgcgcatcg tcgataaata cggcctcaat ctcgaagtga ccaaccccga
      241 tctcgacttc accttccggt tcatgtcggt ggattgggac ggcaagatcc gcatggactg
      301 ttcctctccc tacgccatgg ccgggctggt cgcgctcaag gaccagttcg acatcgcctt
      361 cgcgaatgac accgacgccg accgccacgg catcgtgacg aaatccggcg ggctcatgaa
      421 tccgaaccac tacctcgccg ccgcgatctt ctatctcttc cagaaccgtc ccggctggag
      481 cgcggatgcc ggaatcggca agacgctcgt gagcagcagc atgatcgacc gcgtggcgaa
      541 aaaactgggc cgccggctgg tggaagtccc ggtgggcttc aagtggtttg tggatggcct
      601 ctacaccggc acgctcggtt tcggcggcga ggaaagcgcc ggcgcgtcgt tcctgcggct
      661 ggacggcacc ccgtggacga cggacaagga cggcatcatt ctcggcctgc tcgccgcgga
      721 gatcctcgcc gtcaccggca aggatccctg cgagcattac cagaccctca ccgcggaatt
      781 cggcgcgccg atctacgagc ggatcgatgc cgccgccacg ccggaagaga aaaacaaatt
      841 aaaaaaactt tcccccagcc agctcaaggc cagcacgctc gcgggcgatc gcattaccgc
      901 cacgctcacc gaagcgccgg gcaacggcgc cgccatcggc ggcctgaagg tctgcaccga
      961 aaagggctgg ttcgccgcgc ggccttccgg caccgaggac gtttacaaga tctacgccga
     1021 aagcttcgtg gacccggatc acctcgcccg catccaggcc gaggccaagc agatcgtcgc
     1081 cggcgccatt gcctgagcct cgtcgcggga gctctccccc atgcgcctcc agattaattt
     1141 ccccgccagc cggaccagca actcgttcgc gggcaagctg ggagtcacgc tgttttttgg
     1201 actgtttttc ggattgggga gcgtattctt ctgcctggcc gggatggact ttatccgaac
     1261 cgttcagccc tatttctggt ccccggcgcc ggcgcggatc ctgtcgggcg aggtcgtgcc
     1321 cgacggcgac gatcacaaaa tccacgtccg tttccggtat tcctggcagg gccggacttt
     1381 cgaatcggcc accttttccc agccggagga gagttttaat cactacgaca cggcctttga
     1441 agccctcgcc gcgctgccgg cgggaacgga aaccacgtgc cgggtcaatc cgaaagcccc
     1501 ggaacaggcg gtcctgcggc tgcggggatt gtggatgggg ttcgtgatgt tgattccgct
     1561 catcttcgtc gcggtcggcg gcttgggcat gtggggagcc tgggcccggc ccgcccccgc
     1621 cacgcggcgc tcctcccagc cggtgaaaaa gtcccgggta accggccgag tgaccggcgc
     1681 cgcgctcgcg ctggccggcg ccggcctgtt ggccgcctgg gccgtcccca tgtggctgaa
     1741 agcggccgac tccacgcgct ggccggagac cccctgcgtc attctttcca gctgcgtgaa
     1801 gtcgcattcg gacagcgacg gcaccaccta cagcgtggcg atcgcctacc gctacacgtt
     1861 tgaagggcgg gaataccgct cggatcgttt cgacctcatg aatgcctcct ccagcgggcg
     1921 cgcgggcaag gaagccctcg tcgcgcgtta cccggctggc tccacgcggg tttgctacgt
     1981 cgatcccgac cggccctccc aggcgctgct gcggcgcggg ttcacctgga gcctgctttt
     2041 gggcctgatc ccgctggcac ttctcctttt tggcctcttc ctctgcttcc aaaagctcgg
     2101 caaaacgaac cccgccgcgc aggcgccgcg ggtggacgcc acgggattgc ttaaatccaa
     2161 ggaatcgccc gggctgcggc tgggaggaac gctggctttc gcgctcttct ggaatggcct
     2221 tctctcggtg tttttgctcg acttgattgg cggcttccgc cggggccagc ccgattggtt
     2281 tctcgcgctg ttcctgtgcc cctttgtgct catcggcctc gccgcgcttg gggcggcggg
     2341 ctactacttg ctcgcgctgg gaaacccgcg aatccggctg cggctggaaa ccggccgcat
     2401 cgagccgggc gctccgttga aactcgcgtg gcagatggat ggcgccgtcc gccggcttga
     2461 gcgcctcgtg atttatctcg aaggccgcga ggaggccacc tatacgcgcg gcacctcgag
     2521 cgtcaccgac aaaagggtct ttttccgcca gcccctcgcg gatgtcaccg gcacctggat
     2581 ggatttccag gaaggccgcg gcgccatttc cattccccgg gaggctccac cgagcttccg
     2641 cgccagccac aatcgcattg tctggtcgtg ccaggtttgg gggaaaattc ccttctggcc
     2701 cggcctgcgc gaggaatttg aaatcccgct gcaaccgcct ttccccgccg catgaacgag
     2761 tttaccgcca ccctcgcccc ggaaaaaacg cgcggccggc cgggggatcc gattcgcggg
     2821 cgcgccagct ggcacggtcc gcacccgccc cgctccgccg agctcgtttt attctggcgc
     2881 accgaaggca agggcgaccg ggacagcgaa acggttttcc ggctcgagtt cgaccagccc
     2941 caggccgacg acgaccgccc gttcgccctg accgcgccgc cctttcccca cactttttcg
     3001 ggccagctga tctcgctcat ctgggaactc gaacttctgc tggacgaccg gggagccggc
     3061 tccgtggaaa tcacgctctc ccccttcccc gaagaaatct cgctcgagcg cgccgagtgg
     3121 atcacgatgg acgcgccggg cccccaattc cccttcccct ggcggaaaac gcgatgaagg
     3181 cgcgcgaaaa tccgtttcgc cgcgagcgga tcgaagccct ggcctatcgc gctccggact
     3241 tcgattgggc ggagctggaa gcgcgtctca cccggctcgg cgggcagggc gccctggtcg
     3301 gcccgaaagg ccatggcaaa accacgctgc tggagcaatg ggccgcccgc gtcccgggct
     3361 ccctccattt tcgcatcgcc gaaggacagc gctttctcac cccggcccag cggcaggcgc
     3421 tttgcgagag ccgggctgcc tttgtgctgg tggacagcgc cgagcagctg ggttggctcg
     3481 gctggcggga attgcgccgg cgggttggca atgcgcgccg gctggccatc accacgcacc
     3541 gtcccggccg cctgcccacg ctgcggcatt gccagacctc gccccagttg ctgggcgaac
     3601 tcgccgccga gctcaccggc gtcccgcgcg actgcaccgc gctctggcgg cggcaccgcg
     3661 gcgatctccg ccgggcgctc cgggagctgt atgacgccgg gcattgcaaa aaacctttac
     3721 ccgccccgcg ctctgtggcc tcactgaatg ccgccccaat cccgatatga gcaatcaacg
     3781 catcgtcttt tttttcgaca tcgataacac gctcctcgac aacgaccgcg tcacgaagga
     3841 catcgccaag aagctcgaca cggtcgtggg cgagacggcc cagaagcgtt attgggaaat
     3901 cttcgagcag ctccgcaagg aggagggtta cgcggactac ctggcggcgc tgcagaagtt
     3961 ccgcttcgag gataagtttg atcccaaact catttatctc tccctgttcc tgacggatta
     4021 tccgtttgcc aaccggattt ttcccagcgc gctggatgtc atcgaccggg cgaacgagtg
     4081 gggccaggcg gtcattttga gcgacggcga cgtggtcttc cagccgcgca aggccttccg
     4141 cgccggcctg ctcgatgccg tcggcgggcg cgccctcatt tacattcaca aggaagagga
     4201 actcgccgac gcggaaaaac gctatcccgc cgatcactac gtgatgttcg acgacaagct
     4261 gcgcatcctc gaggcgatga aaaaaatctg gggcgccaaa cttaccacgg tgtttgtccg
     4321 gcagggccac tacgccttcg agccgggcgt ggaagaaaaa tacgcgaagg cggatctgac
     4381 gatcgaccac atcggcgacg cgctgaattt cacccgcgac cagttcattc tcgccgccta
     4441 ccactcatga aatcctccgc ctaccacgtc aaaccgggca gccgggtccg gctcgccagc
     4501 cacgatccgg gcgataccgg gcattacaag gacaagcacg ccgcccgaac ccggctggcg
     4561 gaggacatcc tggagatgca aagactgcag gacatcttct acgccgccga ttcgtattcg
     4621 ctgctgatca tcttccaggc gctcgacgcg gcggggaagg atggcgccat caagcacgtc
     4681 atgtccggcc tcaatccgca gggctgccag gtcgccgcgt tcaagtcgcc ctcggccacg
     4741 gagctggatc acgactacct ctggcgctgc aacatccggc tgccggagcg cggccgcatc
     4801 ggcattttca accgctccta caacgaggac gtcctcgccg tgcgcgtgca tccggaatac
     4861 ctcgccgcgc aaaaattgcc gcccaagctg accggcgcga atttgtggaa gcggcgcttc
     4921 gaggagatca gccattacga aaaatacctc gccgacaacg gcacgcgcat cctgaaattc
     4981 ttcctgcatg tctcgaagga cgagcagaag cggcgcttcc tcgaacgcat cgagaatccc
     5041 gacaagaact ggaagctttc gcccagcgac gtaaaggagc gccagcattg gcacgactac
     5101 gaggcggcct acgaggatct gctctcgcac acgagcaccg actgggcgcc gtggtatgta
     5161 atccccgccg acaaaaagtg gttctcccgc acgctgatcg ccaatgtgat cgttcgcgag
     5221 cttcaaaaac tgaagctgag ctaccccaag gtttcgccgg cgcatctggc cgcgatccag
     5281 gaatccaaag ccgcgctgct ggcggaaaag gactgaccgg gcatgggagc cggcgcctcc
     5341 attgccaatg aactggtcct gggtttgggc ctgttctttc tcggcatggt gctgaccggc
     5401 gacaacctgc gcctgctttc cggccccagc tttcgctccc tcgtcggcaa acccgcccgc
     5461 acgcccgcgc tgggcggact gatcggcctg ggcttcggcg cgctcatgca gagcgccacg
     5521 gcggtcacct tcatcctggc caacatggtc cgcgcccggc tgatcgagct ggcggcggcg
     5581 gtgccgatcc tgctgtggtg caatgtcggc ctgacctcgc tggcgtttgt ggtcaccttc
     5641 gacattcatc cgctggtcgc ctggttcgtc ggcggttcgg gcatcctgct ggggctcgtc
     5701 aagaagcccg gctggcgcgc caccgcgggc gtctgcctgg gcatcggcct gatcctctat
     5761 ggcctggaaa gcatgagcgc gggagccgcc ccgctgcgga atctcgcctg gtttcaagcc
     5821 atcctgcacc tcttcgagcg cgacccgctg tttgcgttcg ccggcggatt cattatcgcc
     5881 gcgctgctgc aatccaatac cggcgcgacg atgctcatca tcacgctctt tcaagccggc
     5941 gcgttttcct cctccatcgc cagcccgctg atctacggcg cgaacctcgg cgccattccc
     6001 ctgcgcaatc tgctggcctt cagccagggc ccggccgcgc gcgggctggc gcgcgtcgaa
     6061 gatgcgttct gcctgctgag cggcctttta atgatggcct tgttttacct ggaggagttc
     6121 gcccgcattc cgctggttca cgcgctggcg gcggcgattt ccccctcgcc caatacgcag
     6181 ctcgccctgg tcttcctgtt ctccaacctt ttgcccgcgg cgttattgag cccgctggga
     6241 gggcgaatcg cccgattcat ccagcaacgg tttccgccgc cttccgagga actcccggcg
     6301 cagagtttcc tgaactccaa ggcgctggat gatccttcaa ccgcgctgga tcttttctcc
     6361 aaggaaatca tccggctgct gaagaccgtc cagccccagc ccatcgaatc cccggagggg
     6421 gaggacgagg aaggccagcc cgataccgcg ttctcggaga tcgcgcagaa gattgaccaa
     6481 ttcggaatcg acctttcgcg caagtcgatg ttttcgcgaa aggacgccca ccggctccac
     6541 ctcctgcgcg cggaattttc catggtgcgt tacatcgagg aaaccgtccg cgccttcaat
     6601 cgtgtcctcg gccaggagga ataccgcaaa cgcatggaaa cggaggcaac ggccatgcgg
     6661 cagtttctgg aaaaaacgct cgcctgggcc ggccaagcgg cggattcgct ggcggagccg
     6721 gacatccagg cgctgcggga gacgacccga aagaccgacc cggactacga ccgcgtccgc
     6781 cagctgggct accggcgacg caacaccgaa tccgccgaaa acaatctctg gctggcctcg
     6841 ctccaagacg atttcgaaat ggccctctgg atgctgcacc gcttttccaa aaacctgacc
     6901 cgccgcgccg aggcctcggc cgcgcgttga acgcaaagct aaatccaaac tcgcgcttct
     6961 caagcccccg gcaagctggt tacttgttcg gatgaatttt ttggaggaac tcggcaaacg
     7021 cgtgatcggt ggcgacggcg ccatggggac gctgttgatg gaacgcggca tcgcgctcaa
     7081 tcaatgcttc gaggaaatct gtctcagcaa tcccgagctc gtgcgcggcg tgcatcgcga
     7141 ctacgcggcc gcgggcgcgg aactcatcga gaccaacagc ttcggcgcca acgccgtgcg
     7201 gctcgcgcgc cacgggctgc agaaatgcgt cggcgcgctc aaccgccagg ccgcccaaat
     7261 cgcccggcag gccgttcatg gcgccccggc gtgggtcgcc ggcagcgtgg ggccgctggg
     7321 cctgaccccc gcccaggccg aggagcaggg gatcgatcgc gcggcggtgt ttcgcgaaca
     7381 gatcgccgcg ctcatcgaag gcggcgtgga cgtcctgctg tttgaaacct tccagagcct
     7441 ggacgaattg cggatcgccc tgcgcgccaa agccgagctt tccgattgtc ccgccatcgg
     7501 ctgcctgacg ctcagcgagg gcggccgcct cgcggatggc gccacgctca cggaggcctt
     7561 ccggcaattg gcggacgccc aggccagcgt cgtcggcatc aattgcgtca gcggcccgca
     7621 ggcggccatc cgcctgctgg aaaatgtcga aagcccgctg ccgctcgcgg tgtatccgaa
     7681 cgccggccgg ccgcggcttt acgaggggcg ctacgtttac gattccagcc ccgaatactt
     7741 cgccgaatcc gcccagcggc tcgccgaact cggcgcgaac ctcgtcggcg gctgctgcgg
     7801 caccacgccc gcgcacatcg ccgccatcag ccggcagttg aaaagcttcg gcccgccggt
     7861 caagccacgc aaaaaaatcg ccgtcgtcga acgggaaaaa atcaaactcg ccgtcgccgg
     7921 ggattcgatc ctcgaccgat tgaaggcggg caagacggtc atcgtcaccg agctcgaccc
     7981 gcccaagacg ctcacgctgg accgttactt cgaggcggcg gaaaaattga ccgaagccgg
     8041 cagcgacgcc atcacgctcg ccgacaactc gctcgccatc ctgcgcgtga gcaacctggc
     8101 catcggcgcc atgctcaagg cgcgcggcat cgagccgttg ctgcacctca gctgccgcga
     8161 caaaaacgtg ctcggcctgc aaagcgagct catgggcgcg gcggcgatgg gcatccgcca
     8221 catcctgccg ctcaccgggg atccggccaa ggtcggcgat caccccggcg ccacgtcggt
     8281 ttacgatgtg aattccatcg gcctgctcga aatcgcccgc cggatgaacg aaggttacac
     8341 gcaggcggga accgatctca aaaagctgcc cggcttcgtc atgggctgca cgttcaaccc
     8401 gaacgcgaaa aatctcgacg cccagatctc gcggctgcag cgcaagatca acgccggcgc
     8461 gcaatacgtg ctgacccagc cggtcttcga cgcgggactc gtccggctcg ccgccgaaaa
     8521 ggtgcggcat ttcaacattc cgatcttctt cggcgtgtgg ccgatcctca acgggcggca
     8581 ggcgaaattc ctgcacaacg aagtccccgg catccgcatt ccggagcccg tgctggcgga
     8641 gatggaagga ctggagggcg cggaaggccg ccggcacggc atcgcgatcg ccaaggaagt
     8701 ctgccgcgcg gcgctggacg aatttccctg cgtgtatctc atcacgccct tccaggttta
     8761 cgaaaccacc gcggagctgg cggagtttgt gcgcggacga acttagcctt ccttcggctt
     8821 ggcgggttca ttgattttcg cctccgcggg aggtgaaccg aagtaaacat ctcccaccgg
     8881 gcttaacccg gatattcgaa aatagacctc cgcgaaccgc gccttggcat taatcgtccg
     8941 cgccccttct ccgggggcaa acaattgata cacctgctgg cagccttgcg tcgccgacgc
     9001 gccgacggat acgttgtcca cggaatttcc cgcctcgtcg aaaaaccgga caccgatcgc
     9061 tcccgacgcg gctccccccg gcgatccgaa gcgcgctgcc aggaggtagt tttgggtcgg
     9121 atcaattttg acacgattcg ccacaatctc cgcctgatct cttcctcttg aggtcgtcgc
     9181 gaagagcatc gaggatccat tgctggtcaa actgttcggg catttcaacc accaaccgtc
     9241 tgatccgcaa ttccagccgg tttttttcgg atcattcgcc gcgagatcct tcggcagttc
     9301 cttaaacgtc ggatttttga gcaggttgcc ggatgggggc acaatgggga tcacgggacc
     9361 gggggtgatt tttccatccg ccgccaccaa atcggctcgg acaaaccgcg tttccgccgg
     9421 aagtttttcc gaccattttc ccatggactt aaccgccgga agatcggccg cttccttcaa
     9481 ctccttcgga ttgggacccg ccaaaatttt cagggtgtat ttaccatcat tcgaactggc
     9541 ccggcggccg gggaaggcag gagtcagctt gctctcatcc accggcttga acgcccatcg
     9601 taattccaac cgcccctcct tcagggtgag ccagagaatc gcaaccgcat tgccgccttc
     9661 cttgtaaaac ttcaattcct cgcgcatgcg ggctaatttc ttcatgtcga atcgtccgcg
     9721 aaaattagcc ggaagaccgc tgacggtggc aaggagacgt tcacattccg cataattttc
     9781 cttggcgatg aggccgttcc agcagggaag aatgatgccg tagggagcat tgtattgtcc
     9841 ctggggaccg gaaagtttga cgatgaagtt tcccagcaat ttctccggcg gaacggacag
     9901 gatgccgcgc accgcatcaa gccggaactt ccagggagcg agggcgtttt taaggaacgg
     9961 cgtattcccc tgggcgtcat cgggtgaatc aatgatccgc tggatccagt ccaacgccgc
    10021 cgattgagtc gccggccagc gcagaagctg gcttaaacaa agcgcgatcg ctccctgagt
    10081 ttgaaaatcc cccgcctttg ctgagtcgaa aggctcccgg ccgagttgcg cgacttccag
    10141 aaccaccgag gcatcccgct ccatcgcccg caatgccagg gccaaaaaac ggtttttctc
    10201 cgagacatcc gccttggcgg aggccgccac gatttcgcgg gcttccttca accaaccttg
    10261 ggtcttggcc aattccagat agcgctgcgc gtcgctggat ggcaatccgt catcactgga
    10321 tccaatgctc gaaaagaagg gatcgaagtc cccataactg ccaaagctcg ccgcacttcc
    10381 caacaagctg ttcgactgcc ggcgagtcgg agcccggctt ccactggagg cctcaaacat
    10441 ctccaacagc agttttttcg cctcatcctt gcgttcctgg gcgaggtatt gctcgatcaa
    10501 tttaagagtc agacttccgc ggcgataagc gtcccgccct tcttgggaga gcttcaatcc
    10561 ctgctgcaac gcccgcaccg cctcgtcctt gcgttccttg cgaatgagca ggtcggacag
    10621 gcattcgaaa taccagccga catccaccga acggccctga ggattggccg gcggaaattc
    10681 gatcgtgatg agttcgtcca atttccccgc gtcctcaaaa agctgaatca tctcgttcgg
    10741 ctcatctacg atcatttcag gatctcgctt gatcacgtcc cgataaaccg ccagcgcctc
    10801 ctcgtcccgg ccctgcgtgc ggagcgcctt ggcaatttgc gtgttaaaaa tcggatcctg
    10861 atttcgcaat tccgccagac gctgccaata atcgagcgcc ttatcgcgag tctgccgcca
    10921 ctgcagcgag gcggaccctt cggccagcca cgtcatcatg atattcgcgt cgttctgccc
    10981 caatccctcg cgaatcatca ccccggcttt cgaccattcg ctcgtccggt ccagcgagtt
    11041 tacccgggca atcatttccc catcgcccgt cagggttcga tacacgtaat ggaatcgatc
    11101 cttgcgcctg aaaatatcct ctccggacgc cgacacccaa attttgccgt cctgcaggac
    11161 ttccttgccg gcgatcttcg gacggccgat gtcccgtgcg gcccaaccgg tttcgggtga
    11221 cacgccttcc gccggttcga cggcgccttt ccaagtcacg tcctggaact ccgccgtggc
    11281 caatttcgcg tccgtgtgag cggtgaccgc gaggccgata taggtcttgg ccggcaggtt
    11341 cagcctggtt tccttgaaga cctcccattg ccggccatcg cgggaaaggc tgccccggaa
    11401 cacctctccc tcgcgctgaa ttttcaacca cagcggggag gaaaatccgt ccttggtgta
    11461 atgcgaggta tccgccgcgc cggtgatgcc atagcccgtc gcgatgagaa acaacagccg
    11521 ctgcgaatca ggcgccgcct ccagctgctt ctcccaagcc cggatttgcc caatgagccg
    11581 cgacatccga ttcagcgcgc cgcacgccgc ctcggccgga ttccattcgt ccggcttata
    11641 cttcgatacc agatcattcg caaaaatctg atccgcgagc ttcatcgcct catcggtttt
    11701 ttcctccctc gccagctgat acagctggtt gacctgctcg ttgagggaga ttcccggatt
    11761 tgcggaagtc gaacccccgc tcgcgggtga gcgaagcggc tgccccgtgt aattggaaag
    11821 tatctgccgt tcctgcggtg aaagccgggc ctgggaaagt tcggcgacca tcttttcaga
    11881 aagcgccttg tcgccggtta tcttcgacgc cgccagcata tcgaactgcg cgcagcgata
    11941 cgtttgccca tcgatcggct tgatttcctg gaggatggcg atggcttccg gattcttccc
    12001 cgcctgcgcg aggattttcg cataaacgag acggatatcc gcatccgagg tctcttccaa
    12061 aagctttcgc gcggcggcgg cggattcctc cttctttcca acccacgaaa gcatgaccgt
    12121 cgcgagcagg cggaccgcaa ctttttgatt ggccggaacc tggggctcca gttgattcac
    12181 catttccatc atctccccgg caacacccgc cgcgcgagcc cgctcgaaaa ccgtgttcaa
    12241 ggcggccagg tccgaagcat cgaaatattt cgtcggaacg atcaaattcg aacctccgaa
    12301 tccgaaaaag ccctgattca tttgcgcccg ggaaatgggt gaaagctttc ggttgaaata
    12361 gtcctcaagc aacacccgcg tcagggccag catctgcttg gaatccggga gggattcgtt
    12421 gttttcctgg aattgatagg tccacgaaag caaatacgcc aggcgatcca tgtaaggcga
    12481 cggggattgg agattcggat ttgcccgcac gcgggcgaga aaatccttga acgcgggaac
    12541 tgcatcaaat ttcaaagcgc cgtacgccgc aaaggcgata ttcatttgcg gatttgcttg
    12601 cagatcgatt tcgcccaggg ccggcttgaa gatcgcgcgc gctttttcca tgtcgcccaa
    12661 tttttggcag gccgacgcca gcgtgaatgc ggaaaaaaag tccttcggat ttcgcgcggc
    12721 cacgcgttcg gtgaattgct tcaagagggc gttttcgacc ccttcgaccg gcttgccgct
    12781 cgccaatacg ctcaggcagt aaaaatccag ctcaggattg gcgggccggt tgttcacgta
    12841 atcctcgacc agcttgaaaa acggttcgtt cattccaagc agggattgca caaatagctt
    12901 ttcctccggc ggcgcatcgc tgctggccag cgcggcctgg gcgcggcttg ccacctccgc
    12961 ttcggcggcc gattccgcgg tcgtggccac cgcccacgcg agcgcgtaat cgcgcaccga
    13021 gattttctcc gaggggctgg gcaacggttg ttgccgcgaa aaagacacga agctgccgcc
    13081 gccggacttc cggctcatca caagctgccg gagctgaagg gtcaacgacg tgagaaagat
    13141 tcgccgctgc agccattcat tctgggacga ctggctgaga gccatcatcg aattcagata
    13201 aaaaacaacc ggcgattgat ttttggacgg aggaggaatc agcaccggcg gcggattcgc
    13261 cacgatggac cagagttttt cgtcaaattc ctttcgcggc agcaccgagc tctccaataa
    13321 gaggttcatg gcaaactcaa aggcatcgac ttcggaccgg ccggtcttgc gattccacag
    13381 ctccaacgcg gccttcgcgg ccgccggctt cttgatggcg ctcaccaacc cataaaagac
    13441 ctccggcttc ccggtccatt ccgcggcatt ttgttcgagc aattgcagcg cctcccccga
    13501 acggtcctcc gcaaagagga tttgggaaaa ttgcaattga ttcgccggcg tcggttccaa
    13561 ctccagcagc cgcttccaat acttcgagcg ttctttcgga ttattctcct gctcagaaat
    13621 ccggatgatc tgccgaacca tccgggcatc attcggccgc gcggcgtaaa cgcgaatcag
    13681 ctgctcgcgg gcgctcgggt aatcattcag ccgcagataa agctgcgaaa gataaagcgc
    13741 gtatcgccat ccatcctcgg actcgccttc ctgccgcttt ttgaagcgct ggatcagttc
    13801 atcaattcga ttcgacagca aatagggatt caccatctcc ccgagaatgg ccacgcgtcc
    13861 ctcgggttcg ctttcggctt caaacgcctc ttcgtaaacc cggatggcgt cggaataatt
    13921 gccgcgctga aaataaaggc gcgccagccg cgacagggga tcgcggggtt tttcggaaag
    13981 cccggccgcc tgggtatacc acccggcggc ttcatcgaat ttgcccatct gcaaggccag
    14041 gttgccggca aacaaatagc catccggctt ggcggggaaa gccgcaatga catcccgagc
    14101 tgcctgcagg gcttgatccg gctggttcgc tttctggtaa ctgttcgcaa cttcctgcag
    14161 ccagccttgc ctttgctcgg aatcacgttc ggccagcttc tttagcaatg cgatggtttg
    14221 cccggtgttt ccggattttc tggcgacctc cattcgtaac tgcaggagcg ggagagaatc
    14281 cggaaacgtc ttttccgccg cggcaagggc cgcctccggc ttgcgatcca aggctgactg
    14341 caaccgcaag agcaggcgga attcgggttc ggacaatgtc tctccattct ccaacctcgc
    14401 tcgcaattcc ccctcctcgc cctccgcttt tttcaaattc tgcaggagcg ataccttttg
    14461 ggtttccgcc tgaaccaacg cggcattgga gggaacccgg gcgatgagct gatccagcat
    14521 cgcgagcgct tcctccatgc gtttttcccg ggtaagaagt tgatagctga gattgagcaa
    14581 atccaggcta tccggatatt tttcaatccc cttcgcggca gtttggatgg cttcgttcac
    14641 cttgccattc ggttcctgca agcgagccag acgaacgtaa tcggacgcct ccgcgttggg
    14701 tttggcggtg aggccttcca gaacgcccca ggccttttcc ggttgtttgg ccttaagccg
    14761 cgaacgcccg agccgctcga tcgtatcgga actttgcgga tccatggcga gggccttttc
    14821 gtaaaattcc gccgcggttt catcaaattc ataaccggat aaaagatccg cgcatttgga
    14881 ataatccgcc gccatcggct tgtcggccgg aaccagggat ttcagagttt ccacgacctg
    14941 agcctgaatc ccggcttccc cggttcgttt aaaatgcgcc cattgaatcc gcgccagggt
    15001 ttccacgagt tctttgcgct ccgggttctg ctggatccat tggcccaccc gtttctccgc
    15061 ttccgcctcc tgcttcgctt cggtcaggcg cgaagccaag acgagcttga tttccatatc
    15121 ctccggagcc cactccgaag cctggcggat ataattcaat gcatccgcgc tctttcccaa
    15181 ctccgcatcc acgttggaca accgcatcgc gctctccact tcattgggat gagtcttcag
    15241 ccgggtttca taatattggg ccagaccggg aagatcatcc gcctggcgga atgaagtctc
    15301 gatccgcgag cgcaattccc gctgcagcca gcttttcggg ctgacgtcct ccagcaattt
    15361 ctcatattcc gccagggcct cggcccgctt gcctgatttt tccagcaagc tggccgccag
    15421 catttccagg cgcagtttgg ccgccgggcc gggagaggaa ttttttaaaa aaccgtcaag
    15481 caattgccgg gcttcttctt gcttgcccgc ttcggcgaac gcggaagccg cctcttccat
    15541 taccaccggg tcatctggat aagccgccgc cacttccttc cagacttgga tggcttgatc
    15601 cgcttgaaaa tttcgctcat acagcaacgc cagctcgcga taaagtccaa gctgatcctc
    15661 ggtatttgac gtcaacggaa tcgcccggcg ataggcttca gccgccttgg ccagatccgg
    15721 cttccgggcc gccagccgcg ccaacgccgt ccacgccgga gccgaagcgg gactagccgc
    15781 cagatgcgtt tcaaaatgcc gggcggcgga ttcgccattg ccccgccgtt cttccagcaa
    15841 tccgagcaat agctcataag aagccgcatt cgcatcccgg ccagccgcaa gcttcgccca
    15901 tttgtcgctt aattccgcca gatttcccgc ctccagataa taaagataca ctttatcaaa
    15961 cgccggcccg ggattgggat tcttgatcag gatttcctca taacgggcga ggacttccgg
    16021 cgacagtttt tccgccctta ttccggcgat cggaaaaagc aaaagcagcc aggcaaggga
    16081 taaggaacgc ataaacccat tcatgagagg atttggggat tgaatcgtga aatggcggaa
    16141 agccggcgga ctccgacgtc cgcgataaat agcagcagag ccagcgccac cagccatggc
    16201 cacaactccc gttcggcagc cgtggactgg ggaggaagct tgtaaatttg ttccggcgcg
    16261 ggatcgtaaa ctccgcgggt gcgttcgctt aattcgcgaa gcaattccgt attcggcagc
    16321 tgcggcaaaa actcattggg gtaaccccgg acaaaacttt cgtattgcga ggcaatgggt
    16381 tcacctttgg atttaagttg aaactgcaca tggtaagccc cgcgccgagg cgctggccag
    16441 cgggcctcta atcgtcccgg cgccgtgcgc tcgagcggaa gtttcaggga ttcgttggaa
    16501 ggagtgatca ccaccgcttc accttcggta tcattcaaaa actctccccg cggtcccacc
    16561 gcatccactg tcagctttaa gccgtcttta tccggacgca attcagccgg caggcgcttc
    16621 aatgaatccg agcgcatcaa ccggcgaatc gtctgcgccc agaaacggct gaattccg
//
LOCUS       DATDAY010000284         4255 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_981437, whole genome shotgun sequence.
ACCESSION   DATDAY010000284 DATDAY010000000
VERSION     DATDAY010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 4255)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4255)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4255
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_981437"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..565
                     /locus_tag="VIS74_05735"
     CDS             <1..565
                     /locus_tag="VIS74_05735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY5792779.1"
                     /translation="FSLGGNLTLKYLGDLGPALDPRITGAAAFSVPCDLASSSRQLEK
                     MTNRVYMGRFLVPLRAKIREKMIRFPGQLDDAGLDAMRTFREFDGAYTAPLHGFQSAE
                     DYWRRASSKPVLSRIAVPTLLVNARNDPFLAPPCFPEEAARENPFFHFEAPRSGGHAG
                     FLSFNARNEYWSESRAVEFLGNGAQRQ"
     gene            968..2185
                     /locus_tag="VIS74_05740"
     CDS             968..2185
                     /locus_tag="VIS74_05740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792780.1"
                     /translation="MDAFFAELGRTVLERWKRENFSLAKFPGIAQAALEERPPAERVD
                     LPAFIREFLLNDEQPPQTHSGFGQPELVVYNHDRFYIQVLFWLDGTTDIHQHEFSGAF
                     HVMAGSSIHAHFAFENEQAVTAHFRVGDVRMTKIELLETGRTVPVVSGRGCIHSLFHL
                     ETPSVTVVVRTQHDPGTGPQFNYLPPHLAVDPDFNDALTLRRRQLLDVQEITEDPGYP
                     ELVREMIAELDFERGFFVLQHGMGHLQTLGEWEAALAAFEKKHGALAAGVAATLEESA
                     WRDGIKNLRGTIVEPEHRFFLALLLNVTTRADLLALVGQRFPEAAPVETVLRWAEELG
                     ETTDFGAAILDARFPETLGVASEEQPDLLLAALRHFLEGGKKTAALRALPAAGLKELR
                     AAFAASSLRVLAG"
     gene            2345..2869
                     /locus_tag="VIS74_05745"
     CDS             2345..2869
                     /locus_tag="VIS74_05745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792781.1"
                     /translation="MSLLACAMRMPERVGKYVRLYFNSGRRAIYEEWQFLEWSDGRLI
                     PKASWHDESPYNNIDPSFAEAEVVGANEKPSVIRITDADSNSEFENAYVISKDGKLCA
                     KLVITWKESLYTHHIGSAYDIERGWLFEKTTGLPRAIYPEGDANLPPLEELATVNVMG
                     DADAVQRFSARPAN"
     gene            3037..4152
                     /locus_tag="VIS74_05750"
     CDS             3037..4152
                     /locus_tag="VIS74_05750"
                     /inference="COORDINATES: protein motif:HMM:NF015597.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LOG family protein"
                     /protein_id="HEY5792782.1"
                     /translation="MPKIRLSGTVLGADDPNRASRARLLYLLFAGGWDIYNSNGDQRI
                     SLSNIERKIVESDAFVFTPGATLEDMFKAISIFVGFQTLDRNLAGKPAIILNEDASWN
                     PFFAVLAHLRKMGTVKQDYANFLLSADSPEAVLETLEFTKSQGSPDAGRHKMGESQAV
                     FREAPVPEDYVGNACVFCSASLEDPHYLADGEELGRLLAENKIGCISGAGRSGIMGAV
                     VKGSVEAGGWAGGSNVPHIIELEGLPDGLASFWLRPDIYTRMEVMIEHSDAFVIFPGG
                     AGTVQELLALMIFKRQKNPLMAGKPVVIFNRKNAVGLPFWDPMIDLLSDLCVPGDFVV
                     AHEMEDILPAIRPGMKKLRERECLVEALAPAGAEIAA"
BASE COUNT          778 a         1326 c         1339 g          812 t
ORIGIN      
        1 gtttagcctc ggcggcaacc tgaccctcaa atatctcggc gacctcggcc cggccctcga
       61 tccccgcatc accggcgcgg cggccttttc cgtgccctgc gacctcgcct ccagctcccg
      121 ccagctcgaa aaaatgacca accgcgttta catggggcgg ttcctcgtcc cgcttcgcgc
      181 aaagatccgg gaaaagatga tccggtttcc cggccagctc gacgatgccg gcctggacgc
      241 catgcgcacc ttccgggagt tcgacggggc ctacaccgcg ccgctccacg gattccaaag
      301 cgcggaggat tattggcggc gcgcgagcag caaacccgtg ctctcccgca tcgccgtccc
      361 caccctgctg gtcaacgccc gcaacgaccc ctttctcgcc ccgccctgtt tcccggaaga
      421 ggcggcccgg gaaaatccct ttttccattt cgaggccccc cgcagcggcg gccatgccgg
      481 cttcctgtcc ttcaacgccc ggaacgaata ctggtcggaa tcccgcgccg tggagttttt
      541 gggaaatgga gctcagaggc agtgaagttc gacgtcacgc tcagcccatc catccgccaa
      601 acggcttcgc cgttcattta cttccgatgc ggaaccaccc cctccgccag catcggatag
      661 tgtttttgat tcagcacccc gcagggtcaa gccgtgcaat tccgccgttg ccgcgatcag
      721 gcgcaagtcc gcatgcctgt tatccacggt ccaattccgg ctttcggcac cccctctcca
      781 agaaagcgct gattaaatcc agaggaagaa gaatcatccg cagatgacgc tgatttccgc
      841 agataaaacc gaggctttta aaaaatctgc gttaatctgt gaaatccgcg gattaaatcc
      901 gcggttccct aatggcccgc gcgaatgcgt cttgtgaccc cgcgaccctt gatgggacac
      961 tgggcgcatg gatgcatttt tcgcggaact cgggcgcacg gttttggagc ggtggaagcg
     1021 ggagaatttt tcgctggcga aattccccgg gatcgcccag gcggccctgg aggaacggcc
     1081 tccggcggag cgggtggacc tcccggcgtt catccgcgag ttcctgttga atgacgagca
     1141 gccgccccag acgcactccg gtttcggcca gccggagctg gtggtttaca atcacgaccg
     1201 gttttatatc caggtgttgt tctggctgga tggcacgacg gacatccatc agcacgagtt
     1261 ctccggcgcg ttccacgtga tggccgggtc gagcatccac gcgcatttcg cgtttgagaa
     1321 tgagcaggcg gtcaccgcgc acttccgcgt gggcgatgtg cggatgacga agatcgagct
     1381 gctggagacc gggcgcaccg tgccggtcgt ctcgggccgc ggctgcatcc actcgctctt
     1441 ccatctggag acgccctcag tcaccgtggt cgtgcgcacg cagcatgatc ccgggacggg
     1501 tccgcaattc aattacctgc cgccgcacct cgcggtggac ccggatttca acgacgccct
     1561 caccctgcgc cgccggcagc ttctggatgt gcaggagata accgaagacc cgggctaccc
     1621 ggagctggtg cgggagatga tcgcggaact ggacttcgag cgcgggttct tcgtcctgca
     1681 gcacggcatg gggcacttgc agacgcttgg cgaatgggag gcggcgctcg ccgccttcga
     1741 aaaaaaacac ggcgcgctgg ccgcgggcgt ggcggcgacg ctggaggaga gcgcgtggcg
     1801 ggatgggatc aagaacctgc gcggcacgat cgtggagccc gagcatcgat tcttcctcgc
     1861 gttgctgctg aatgtgacga cgcgggccga cctgctcgcg ctggtgggcc agcggttccc
     1921 ggaggccgcg ccggtcgaga ccgtgctgcg ctgggcggag gaactggggg agaccacgga
     1981 tttcggcgcg gcgatcctgg acgcgcgttt cccggagacg ctgggcgtcg cgagcgagga
     2041 acagccggac ctgctcctcg cggcgctgcg gcactttttg gaaggcggga aaaaaaccgc
     2101 cgccctgcgg gcgctcccgg ccgcggggct gaaggaattg cgcgccgcgt ttgccgcctc
     2161 gagcctgcgc gtcctggcgg gatgacgccg ggctcgattc cggccggcgg attgatcgag
     2221 gtgcgggcca tccccgcggg caggtcgata cctgcgcttt ccggcttccg gcgctcatga
     2281 agccgcgtta caattcgctc caatgcgcaa gttcctgctg cgctttcagc cgaagacgca
     2341 ttccttgagt ttgctggcct gcgcgatgcg gatgccggaa cgcgtgggca aatacgtgcg
     2401 gctctatttc aactccggac gccgcgcgat ttatgaagag tggcagtttc tcgaatggtc
     2461 ggacgggcgg ctgattccca aggcgtcctg gcatgacgaa agtccttaca acaatatcga
     2521 cccatcgttt gccgaggctg aagttgtggg ggcaaacgag aaaccgagtg tcattcggat
     2581 tacggatgcc gacagcaatt cggaattcga gaatgcttat gtcatttcca aggacgggaa
     2641 gctctgcgcg aagcttgtca ttacgtggaa ggagtcgcta tacacccatc acatcggttc
     2701 agcttacgat atcgaacgcg gctggctgtt cgagaaaacc accggtctcc cccgggcgat
     2761 ctaccccgaa ggcgatgcga accttcctcc cctcgaagag ctggcgaccg tgaacgtgat
     2821 gggtgatgcg gacgccgtgc aaaggttttc cgcgcggccc gccaattgaa atcttttcgg
     2881 attggatgac ggatggaaaa tcacggacgg ctcggcgagc cgtccctacc cgggatacaa
     2941 gggcgggttc gatccatctg ccgtgacctg gcaaaaccaa ggggctcgct ttcggcacaa
     3001 taactgctac atcactctcc cgtagttaga cccgacatgc caaaaatcag actctccgga
     3061 accgttcttg gcgcggatga cccgaaccgc gcttcccgcg cgaggcttct ctacctcctc
     3121 tttgcggggg ggtgggatat ctataattcc aacggcgacc agcgcatctc gctttcgaac
     3181 atcgaaagga aaatcgtgga atccgacgcc tttgtcttca ccccgggggc cacgctggag
     3241 gacatgttca aggcgatctc cattttcgtc ggcttccaga cgctggaccg caatctggcc
     3301 gggaagccgg ccatcatttt gaacgaggac gcttcctgga atcccttctt cgccgtcctg
     3361 gcccacctgc ggaaaatggg cacggtgaag caggattacg caaatttcct gctctcggcg
     3421 gattcgccgg aggccgtgct ggagacattg gagttcacca agtcgcaggg ctcgccggat
     3481 gcggggcgcc ataaaatggg ggaaagccag gcggttttcc gcgaagcccc ggtgccggag
     3541 gattacgtcg gcaatgcctg cgtcttctgc tccgcctcgc tggaggatcc ccactacctg
     3601 gcggatggcg aggagctcgg acgcctgctc gcggaaaaca agatcggctg catctccgga
     3661 gcgggccgtt ccggcatcat gggcgccgtg gtcaagggat cggtcgaggc cggcggctgg
     3721 gccggcggct cgaacgtgcc gcacatcatc gaactcgagg ggctgccgga cgggctcgcc
     3781 agcttctggc tgcggccgga catctacacc cgcatggagg tcatgatcga gcactcggac
     3841 gccttcgtca tctttcccgg cggcgccggc acggtgcagg aactcctggc attgatgatt
     3901 ttcaagcggc agaaaaatcc gctcatggcg ggcaagccgg tggtgatctt caaccgcaag
     3961 aatgcggtgg gattgccttt ctgggatccg atgatcgatc tcctgagcga cctgtgcgtt
     4021 ccgggcgatt ttgtcgtcgc ccatgaaatg gaggacatcc tgccggcgat ccggcccgga
     4081 atgaaaaaac tccgggagcg ggagtgcctg gtcgaggcgc tcgccccggc gggggcggaa
     4141 atcgccgcct gacgttgccg gtgattgtag gggcggttgt gccaaccgcc cagcgtaaac
     4201 ggggatgcgg ggtccccgtt tccccgcaac caaaggcgcc caatggggtg gtggg
//
LOCUS       DATDAY010000285         6004 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_1676839, whole genome shotgun sequence.
ACCESSION   DATDAY010000285 DATDAY010000000
VERSION     DATDAY010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 6004)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6004)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6004
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1676839"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..590)
                     /locus_tag="VIS74_05755"
     CDS             complement(<1..590)
                     /locus_tag="VIS74_05755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792783.1"
                     /translation="MNRETLVGYYDKLAALVEKLPGGLQKPIMHELRPIREVFLDQRP
                     ARILVLGEPEITVPEFLCRLAHLDLLESGKTEQGWRRYHSSGRGNIQVLDGRDIGPEW
                     VRSAVQAQAPDLILLLRGDRTPENWNELITQAKLVSGIEPSKLNAKPPENPVPMVGVL
                     TAEKNPATEQAFRAQLAAAGLEEVPIFALAPEDRELL"
     gene            complement(705..1190)
                     /locus_tag="VIS74_05760"
     CDS             complement(705..1190)
                     /locus_tag="VIS74_05760"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEY5792784.1"
                     /translation="MSIALKLGDAAPDFAASVIGGEYGEGKTVQLSGFRGREVVLYFY
                     PKDDTPGCATQACGIRDKWEKLRGSDALVFGISTDSLASHRKFIEKFSLPFPLISDED
                     KSIVNAYGVWVEKSMYGKKFMGTERSTFIIRPDGTIKGIFRKVVPGEHVDTLLEELGV
                     S"
     gene            complement(1289..2704)
                     /gene="fumC"
                     /locus_tag="VIS74_05765"
     CDS             complement(1289..2704)
                     /gene="fumC"
                     /locus_tag="VIS74_05765"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF008909.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HEY5792785.1"
                     /translation="MAHAEIPYDPATHRLESDTMGQIPVPNDKYWGAQTARSLIHFNI
                     GNDIKPPELIKALGILKKACALANHELGKLSKEKCDLIVRAAEEVIDGKLDEHFPLRI
                     WQTGSGTQSNMNANEVISNRAIEIAGGVKGSKKPVHPNDDVNLSQSSNDTFPTAMNIA
                     AAMQVNERLLPELKALRDTLAEKAAEFKEVVKIGRTHLQDATPLTLGQEFSGYVTQLT
                     QDIQRIVESLPGLYSLAIGGTAVGTGLNSHPKFGDTAAKYIAEITELPFIAAPNKFAA
                     LASHNEFVYASGALKSLACSLMKIANDVRWLASGPRCGLGELTIPENEPGSSIMPGKV
                     NPTQSEAITMIAVQVMGNDGAIGIAASQGNFELNVFKPVIIHNFLHSTRLLADGCASF
                     REHCAIGIEANLEKIEEYVKDSLMLVTALNPHIGYDKSAQIAKKAHKEKTGLKQAALA
                     LGYLTSDQFDQWVVAKEMTHP"
     gene            complement(2807..3580)
                     /locus_tag="VIS74_05770"
     CDS             complement(2807..3580)
                     /locus_tag="VIS74_05770"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006983791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEY5792786.1"
                     /translation="MNTETLDLTGMTRRRAFRLGLATLTASLLPWKNGFAQTESAAPT
                     PAAAPTGPYTLPKLPYDYTALEPSIDALTMQIHHDKHHQAYVTNANKLLAGHGELSKL
                     SPEELIANLDKVPEDIRTGLRNNAGGHVNHTLFWQMMSPSGGGKPAGEIAKAIDASFG
                     SFEDFQKQFNEAATKRFGSGWAWLIYRPADTKLAILSTANQDSPLMEPGQLPVLGLDV
                     WEHAYYLKYQNRRPDYIATWWNVVNWGFVNKLFAQATQK"
     gene            3698..4066
                     /locus_tag="VIS74_05775"
     CDS             3698..4066
                     /locus_tag="VIS74_05775"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HEY5792787.1"
                     /translation="MSVTNSATSFQERVYAVTSEIPRGKVASYGFLAKQLNCGSARAV
                     GQALQRGPCAPDVPYHRVVAADRSIHGFSGQIKGAPVDRKRRLLEAEGVRFEAEGRIA
                     PECMLSPESPAHRKKKRRGE"
     gene            4116..4343
                     /locus_tag="VIS74_05780"
     CDS             4116..4343
                     /locus_tag="VIS74_05780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792788.1"
                     /translation="MKVTVDIPEKDLADIMRYSGEKKKGPAIVKFLTSGLMLRRRREL
                     TEEIVSGKWVIDLPDWRITRARERDKNSWAR"
     gene            4331..4735
                     /locus_tag="VIS74_05785"
     CDS             4331..4735
                     /locus_tag="VIS74_05785"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HEY5792789.1"
                     /translation="MGSLIDTSVWIDAFHSKSPPSVKAMASAVINRIDAVVCEPVHLE
                     FFRGIPDRELPRAQQYFDTFPMLSTPPSLWREATDLLRKCLRSGSPVNPLDALISIIA
                     ITHDATIVTFDHDFLILKQHSKLSVELLARVS"
     gene            5150..5563
                     /locus_tag="VIS74_05790"
     CDS             5150..5563
                     /locus_tag="VIS74_05790"
                     /inference="COORDINATES: protein motif:HMM:NF013629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HEY5792790.1"
                     /translation="MKTDRKSHYEETISHKGHRLTPQRRQVYDVLLENRDHPTATEVF
                     IRAKQRMPTISLATVYNCLETLVDCGLAVQVNVEREATRFCPNLDEHGHFVCSHCGRV
                     YDVPMSPRNAVVPLEGFTITHSEITLRGVCANCNS"
     gene            5573..>6004
                     /locus_tag="VIS74_05795"
     CDS             5573..>6004
                     /locus_tag="VIS74_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY5792791.1"
                     /translation="MSLSIQNLRASIGDQEILKGLNLEIPAGEVHAIMGKNGSGKSTL
                     AKIMAGHPSYTVTGGDVLLDGESLLDKEPEERSQAGLFLAFQYPMEVPGVSIANFLRA
                     ALQARKPDEELDAVAYYEQLYAKMDALKIPRNFTARSVNEGF"
BASE COUNT         1193 a         1721 c         1695 g         1395 t
ORIGIN      
        1 agcaattccc gatcctccgg cgccagcgca aaaatcggca cttcctccaa cccggccgcc
       61 gcgagttgcg cccgaaacgc ctgctccgtt gccggatttt tctccgccgt caaaactccc
      121 accatcggca cgggattttc cgggggcttt gcgtttaatt tggaaggttc aattccgcta
      181 actaactttg cttgagtaat tagttcattc cagttttcgg gtgttcggtc gcctcgcagc
      241 agcagaatca gatccggcgc ctgggcctga accgcggagc gcacccattc cggcccaata
      301 tcgcggccat ccaaaacctg gatgtttccc cggccgctgg agtgataacg ccgccagccc
      361 tgctcggttt ttccactttc cagcaggtcc agatgggcca gccggcagag gaattcggga
      421 acggtgattt cgggttcgcc gagcaccaga atgcgggccg gacgttggtc cagaaagact
      481 tcgcgaatgg ggcgaagctc gtgcatgatg ggtttctgca atccgcccgg cagtttttcc
      541 accagggcgg ccagcttgtc ataatacccc accagcgttt cgcgattcat aaatcgtcca
      601 cagattcgca tagatttacg ccgatgggtg gaaaatcgtc accggaaccc aaaaatcggc
      661 tttgcattcg ccattttcct cggggttcaa aatccgtccc gccctcagct cacgccgagt
      721 tcttcgagca gcgtatccac gtgttcgccg ggcaccacct tgcggaagat gcccttgatg
      781 gttccatccg gccggatgat aaacgtgctg cgctccgtgc ccataaactt cttgccatac
      841 atggacttct ccacccagac gccataagcg ttgacgatgc ttttgtcctc gtcggagatc
      901 aacggaaacg gcagcgaaaa cttctcgatg aatttccggt ggctggcaag ggagtccgtg
      961 ctgatgccaa aaaccagggc gtccgacccc cgcagcttct cccatttatc ccggatgccg
     1021 caggcctggg tcgcgcagcc cggcgtgtcg tccttcgggt aaaaataaag caccacttcg
     1081 cggccgcgaa agccgctgag ctggaccgtt tttccctcgc cgtattcgcc gccgatcacc
     1141 gaagccgcaa agtccggagc cgcgtcgccg agtttgagtg cgattgacat aaactaaaca
     1201 ggatttcggg atttacagga ttaactggat tttaggttaa ggatttttcc gttaatcctg
     1261 ccaatccagt taatcccgtt tagttggatc agggatgcgt catctccttg gcgacgaccc
     1321 attggtcgaa ttggtcgctc gtgaggtagc cgagggcaag ggcggcttgc ttcaaaccgg
     1381 ttttctcctt gtgcgccttt ttggcgatct gggcggattt gtcgtaacca atgtgcggat
     1441 ttaaagccgt cacgagcatc aacgaatctt tcacgtattc ttcgattttt tcgagatttg
     1501 cttcgatgcc aatcgcgcaa tgctctcgga aagaggcgca tccgtcggca agcaaacggg
     1561 tggaatgcaa gaaattgtga atgatcaccg gcttgaagac gttcaactcg aaattgccct
     1621 gggaggcggc gatgccgatc gcgccatcgt tccccatgac ctgaacggcg atcatcgtaa
     1681 tcgcctcgga ttgagtggga ttcacctttc ctggcataat ggatgagccg ggttcgtttt
     1741 ccgggatggt cagttcaccg aggccgcacc gcgggccgga ggccagccag cgaacgtcgt
     1801 tggcgatctt catcagactg caagccaggg atttgagggc gccgctggcg taaacgaatt
     1861 cgttatgcga ggcgagggcg gcaaacttgt tcggggcagc gatgaatgga agctccgtga
     1921 tttcagcgat atacttcgcc gcggtatcgc caaatttggg gtgcgagttc aggcccgttc
     1981 ccaccgccgt gccgccgatg gcgagcgaat acaagccggg cagcgattcg acaatccgct
     2041 ggatatcctg ggtcaactgc gtcacgtaac cggagaactc ctggccgaga gtcagcggag
     2101 tggcatcctg caaatgggtg cgtccaattt tgacaacctc tttaaattca gccgcttttt
     2161 cagccagagt atcacgcagt gccttgagtt cgggaagcag tcgctcattg acctgcatgg
     2221 cggcggcgat gttcatcgcg gtcgggaatg tgtcgttcga ggattgcgac aggttcacgt
     2281 catcgttcgg atgaaccggc tttttcgacc ccttcacccc gccggcgatt tcgatggcgc
     2341 gattcgagat gacctcgttc gcgttcatgt tgctctgcgt gccgctgccg gtctgccaga
     2401 tgcgcagggg gaaatgttca tcaagtttgc cgtcgatgac ctcctccgcc gcgcggacaa
     2461 tgagatcgca cttttccttc gagagcttgc cgagctcgtg gttggccagg gcgcaggctt
     2521 tcttcaggat gccgagcgcc ttgatcaact ccggcggctt gatgtcgttg ccgatgttga
     2581 aatggatgag cgaccgggcg gtctgggcgc cccagtattt gtcgttcggg acgggaattt
     2641 gccccatcgt gtctgattcg aggcggtgcg tggcgggatc gtaagggatt tcggcgtggg
     2701 ccatggacta aacaggatta caggattttc gggattaaca ggattttttt gagattattt
     2761 ccccatcatc ctgttaatcc tgcgaatccc gtcaatccgg tttagtttac ttctgcgtcg
     2821 cttgcgcgaa gagtttgttc acgaagcccc agttcacgac gttccaccag gtcgcgatgt
     2881 aatccggccg gcggttttgg tatttcaaat aataggcgtg ctcccagaca tcgagtccca
     2941 gcaccggcag ctggccgggt tccatgagcg gggaatcctg attcgcggtc gagaggatcg
     3001 cgagcttggt gtccgcggga cggtaaatca accacgccca gccgctgccg aagcgcttgg
     3061 tggcggcttc attaaattgc ttctggaagt cctcgaagga gccgaagctc gcgtcgatgg
     3121 ctttcgcgat ttcgcccgcg ggcttgccgc cgccggaggg ggacatcatt tgccagaaga
     3181 gcgtatggtt cacgtggccg ccggcgttgt ttcgcaggcc ggtgcggatg tcctcgggaa
     3241 ccttgtcgag attggcgatg agctcctcgg gcgagagttt ggagagctcg ccgtggccgg
     3301 cgagcagctt gttggcgttg gtgacgtaag cctggtgatg cttgtcgtgg tggatctgca
     3361 tcgtgagcgc atcaatggat ggctccagcg cggtgtagtc gtagggcaat ttgggcaggg
     3421 tgtagggtcc ggtgggcgcg gcggccggcg tcggcgccgc ggactcggtt tgcgcgaatc
     3481 catttttcca aggcagcaag ctcgcggtga gcgtggcaag gccaagccga aaggcgcggc
     3541 ggcgggtcat tccagtgaga tcgagagttt cggtgttcat aggatttcag aaggttaacc
     3601 ctgaaggaca tggattgcag gaaggttttg agattttctt catggtcttc atgtccttca
     3661 tggtaggatt tactcagcat cgtttcccat gaaggacatg agcgttacaa attcagcgac
     3721 ctcctttcag gagcgcgtct atgccgtgac gagcgagatt ccgcgcggca aggtcgcctc
     3781 gtatggtttt ctggccaaac aattgaactg cggaagcgcc cgcgccgtgg gccaagcgtt
     3841 gcagcggggt ccctgcgcac cggatgtgcc ctatcatcgc gtcgtggcgg cggatcgctc
     3901 catccatggc ttcagcggtc agatcaaagg cgcgccggtt gatcggaagc ggagactact
     3961 cgaagccgaa ggcgttcgtt ttgaagccga gggccgaatc gcgccggagt gcatgctgtc
     4021 tcctgaatcg cccgcccatc gaaaaaagaa gcgccgcggg gaatagcttt tcccattcgg
     4081 cttgatctct tttaggattc tgccaccttt tttttatgaa agtgacggtg gacattccgg
     4141 aaaaagacct cgcggatatc atgcggtatt ccggcgaaaa aaagaaaggg ccggccattg
     4201 tgaaattcct gacatccggt ttgatgcttc gccgccgccg ggaactcacc gaggagattg
     4261 tttccggaaa atgggtcatt gatctgcctg attggagaat aacccgggct cgtgagcgcg
     4321 acaaaaattc atgggctcgc taattgacac ctccgtctgg attgatgcgt tccattccaa
     4381 gtccccacca agtgtcaaag ccatggcatc agcggtaatc aatcggattg atgccgtcgt
     4441 atgcgagccc gttcatttgg agttttttcg aggcattcct gaccgagagc ttccgcgcgc
     4501 tcagcaatat tttgacacgt ttcccatgct ttccacgcct ccgtctcttt ggcgtgaagc
     4561 caccgatcta ttacgaaaat gtttgcgatc cggctcaccc gtgaatccgc tggacgcgct
     4621 catttccatc atcgcgatca cccatgatgc caccatcgta acctttgatc acgacttcct
     4681 catcttaaag caacactcca aactttccgt ggagttgctt gcccgtgtgt cctgaattgc
     4741 acggtgaatc accgttgctt caccggtcca gaaaatggat ccgtatattt ccccaccgca
     4801 tcgttccatc cgttcgctca atcaccttca tggtttctct cggtgcgctt cgtctccgcc
     4861 ggtttcgcct ccttgagcat cccttcggcc gcgccacgat ccttttactt tttccgcagt
     4921 gggttgtcgc gtggtctccg cttcctcctc ctcgttttgt gccgccacaa ctccggtgaa
     4981 aagccccaat acccctgccc tcatcaacac ccgccagaag tttttcattg ccatgtcgcc
     5041 tcttacccct ctccattccg gctgtggaag cccatttcaa ctttcgcaac cttctattta
     5101 gaataattct tgattaagac tgcgtcgcag gtaggttacg ctgtccgcga tgaaaaccga
     5161 ccgcaaaagc cattacgagg aaacgatcag ccacaagggc catcggttga cgccgcagcg
     5221 gcgccaggtt tatgacgtgc tgctcgaaaa tcgcgaccac cccacggcga ccgaggtttt
     5281 catccgcgcc aagcagagga tgccaacaat atctctggca acagtttaca actgtctgga
     5341 aacgctcgtg gactgcggcc tcgccgtgca ggtgaatgtc gagcgggagg cgacccgttt
     5401 ttgcccgaac ctcgacgaac acgggcactt tgtctgcagc cattgcggcc gggtttacga
     5461 cgtgcccatg agcccgcgga acgccgtcgt gccgctggaa ggtttcacca tcacgcattc
     5521 cgaaatcacc ctgcgcggcg tctgcgccaa ctgtaactcc tgaattttca ctatgagtct
     5581 aagcatccaa aatctccgcg ccagcatcgg cgaccaggaa atcctcaagg gcctcaatct
     5641 cgaaattccc gccggcgagg ttcacgccat catgggcaaa aacggctcgg gcaaaagcac
     5701 gctggccaag atcatggccg ggcatccgag ctacaccgtc accggcggcg acgtgctgct
     5761 ggacggcgaa agcctgctcg acaaggagcc cgaggagcgc tcgcaggccg gactgttcct
     5821 ggcgtttcaa tacccgatgg aagtccccgg cgtgagcatc gcgaatttcc tgcgcgccgc
     5881 gctccaggcc cgcaagcccg atgaggaact cgacgcggtc gcgtattacg aacagctcta
     5941 cgcgaagatg gacgcgctca aaattccgcg gaacttcacc gcgcgttccg tgaacgaagg
     6001 tttc
//
LOCUS       DATDAY010000286         2197 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_976300, whole genome shotgun sequence.
ACCESSION   DATDAY010000286 DATDAY010000000
VERSION     DATDAY010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2197)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2197)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2197
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_976300"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..221
                     /locus_tag="VIS74_05800"
     CDS             <1..221
                     /locus_tag="VIS74_05800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792792.1"
                     /translation="LAMLFSLKQRIAYSQQAPLLSVRDVVELLEHYLPRAQKTEAEVL
                     AARAKRHRARIAASASARKRNLTQILTK"
     gene            282..>2197
                     /locus_tag="VIS74_05805"
     CDS             282..>2197
                     /locus_tag="VIS74_05805"
                     /inference="COORDINATES: protein motif:HMM:NF015410.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF294 nucleotidyltransferase-like
                     domain-containing protein"
                     /protein_id="HEY5792793.1"
                     /translation="MIYCYGKIGAAYCASPAHLVSRVSTVQTSAIHQRVADFLKEFPP
                     FSYLDDAARERLANHGKVLFRERDDLIFEEGAPRGRFVYLIRQGSVRLSQKRAEEDAL
                     MDLRGEGELLGLSGPLSAPAYSRSAHALEDTILYALDTATFNRECEQNPRATRFLSIL
                     FAADPLDPETGPEGRMVNWFREPGPPPDWVHSRLMTASPDLPVREAARKMVSVEDGSI
                     LVIDEAGRPIGICTDFDLRRRVATGEVPVAALVRELMQAPVITIPPGKSVGESLLEMM
                     RQSARHLCITEDGTPATRALGILSERDLMLFYGNNPLTVLRSIRIARDVETLATLRNR
                     AEALVRMGLRSFSDVDWYCEIVTEINRALIRRVVELVRQKRGESCAAGACLELLGSAG
                     RREMLARTEMGIALFFRDASPAARAEGGELLRELDDALRRCGFAQPPSTICGEEEMEC
                     RTAAEWEELFRGWVIHPIENQIYKRLEFFDFDPLEKGCAVGERVRESLRGILRESPVF
                     TALLANDSLAHLPPLTIFEGSAVDEKGRPIVELDLNAHALRAVSDAARVLALEAGDLT
                     ARSTLSRLDRARERLPAAGDVLTSAARAFRIVLFQRTMNAFQTGGNGNVLSPAALSRT
                     DQALLKFSFHAIARL"
BASE COUNT          367 a          695 c          743 g          392 t
ORIGIN      
        1 gcctggccat gctctttagc ctcaagcaac gcatcgccta cagccagcaa gcgcccctgc
       61 tcagcgtgcg tgatgtagtg gagttgctgg agcactacct gcctagagcg cagaaaaccg
      121 aagccgaagt gctcgcggcg agggcgaaac gacatcgcgc acgaatcgcc gcctcggcct
      181 ccgcacgaaa aagaaacctg actcagatat taacaaagta gaactaaacg aagcctgcca
      241 tatccttacg ccccgatccg ttttgtggcg attttgggtt cgtgatctac tgctacggga
      301 aaatcggcgc cgcgtattgc gcgtcgcccg cgcatctggt aagccgggtt tcgacggtgc
      361 aaacatcggc cattcaccag cgggttgcgg attttctgaa ggagtttcct cccttcagct
      421 atctcgacga cgccgcgcgg gagcggctcg cgaatcacgg gaaggtgcta ttccgcgagc
      481 gggacgacct gattttcgag gagggcgcgc cgcgcgggcg gttcgtttac ctcatccgcc
      541 agggaagcgt gcggctctcg cagaagcgcg cggaggagga cgcgctcatg gatctgcgcg
      601 gggaggggga gctgctgggc ctcagcggtc cattaagcgc gccggcctac agccgctccg
      661 cccatgcact ggaggacacg attctttacg cgctcgacac ggccaccttc aaccgcgagt
      721 gcgagcaaaa cccgcgcgcc acgcggtttc tttccattct tttcgccgcc gatccgctgg
      781 atcccgaaac gggcccggaa ggccggatgg tgaactggtt tcgcgagccc gggccgccgc
      841 cggattgggt gcattcccgc ctgatgaccg catcgccgga tttgccggtg cgggaggcgg
      901 cccgcaaaat ggtctcggtc gaggacggct cgatcctggt gatcgacgag gcgggccggc
      961 ccatcggcat ctgcacggac ttcgatttgc gccgccgcgt ggccacgggc gaggtgccgg
     1021 tggcggcgct ggtgcgggag ctgatgcagg ctccggtcat caccatcccg ccgggaaaat
     1081 ccgtgggcga gagtttgctg gaaatgatgc ggcaaagcgc gcggcatctc tgcatcaccg
     1141 aagacggcac gcccgccacc agggcgctgg gcatcctttc cgagcgggat ctgatgctgt
     1201 tctacggcaa caatccgctc accgtgctgc gctccatccg aatcgcgcgg gacgtggaga
     1261 cgctggcgac cttgcggaac cgcgccgagg cgctcgtgcg aatggggctg cgctcctttt
     1321 ccgacgtgga ctggtattgc gagatcgtga ccgagatcaa tcgcgcgctc atccgccgcg
     1381 tggtggaatt ggtccggcaa aaacgcgggg agtcctgtgc cgcgggcgct tgcctggagc
     1441 tgctgggcag cgcgggccgc cgcgagatgc tggcgcggac ggaaatggga atcgcgctgt
     1501 tttttcggga tgcctcgccg gcggcccggg cggagggcgg cgagctcctc cgggagctgg
     1561 acgacgcgct gcggcgctgc gggtttgccc agccgccttc cacgatttgc ggggaggagg
     1621 aaatggaatg ccgcaccgcg gcggagtggg aggagctctt tcgcggctgg gtgattcatc
     1681 ccattgaaaa ccaaatctac aagcgcctgg aatttttcga ttttgacccg ctggaaaaag
     1741 gctgcgcggt gggcgaacgc gtgcgcgaaa gcctgcgcgg gattctgcgg gagtcgccgg
     1801 tcttcaccgc gctcctggcc aatgattcgc tcgcgcacct gccgccgctg accattttcg
     1861 aaggctccgc cgtggacgaa aagggccggc ccatcgtcga gctcgacctc aacgcccacg
     1921 cgctccgcgc ggtttccgat gccgcgcggg tgctggcgct ggaggcgggg gatttgaccg
     1981 cccgttcgac gctctcccgg ctggaccgcg cccgggagcg gctgcccgcg gcgggcgacg
     2041 tcctgaccag cgcggcgcgc gcgttccgca tcgtcctgtt tcagcggacg atgaacgcct
     2101 ttcaaaccgg cgggaatggc aacgtgcttt ccccggcggc gctctcgcga acggatcagg
     2161 cgctgctgaa attcagcttt cacgccattg cccggct
//
LOCUS       DATDAY010000287         2289 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_194647, whole genome shotgun sequence.
ACCESSION   DATDAY010000287 DATDAY010000000
VERSION     DATDAY010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2289)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2289
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_194647"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1041)
                     /locus_tag="VIS74_05810"
     CDS             complement(<1..1041)
                     /locus_tag="VIS74_05810"
                     /inference="COORDINATES: protein motif:HMM:NF015557.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HEY5792794.1"
                     /translation="MTIAFVLSLLVIAMILFATEKLSVDLITFLMLLALLFTGILTPQ
                     EAFAGFGSDIILILGSVFVISGAMRETGVVDWVGDALSRFAKGGKNPFLTLMMGSVSG
                     LSAFMNNTTVTALMLTPVIGMARRRKISASKLLMPMAFASILGGTCTLIGTSTNVAVS
                     GFLTQSGERPIGFFELTPIGLLIVAIGILYMRFFGQKLLPDHAPVQELTEDYGLREYL
                     SEILIRENSPLAGQPLSHSDLGKMDFRVLRVIREGKSLAPHPRLKLAAGDLLLVNGAA
                     ESLMKVRETEGIEIKADFKFGDKALTSDRQQILELIVTPHSTLRGRRIMDLDLPARQG
                     IVVLAINRHGQHL"
     gene            1174..1941
                     /gene="mazG"
                     /locus_tag="VIS74_05815"
     CDS             1174..1941
                     /gene="mazG"
                     /locus_tag="VIS74_05815"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF007113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HEY5792795.1"
                     /translation="MSEAVGRLTEIVRTLRSPEGCPWDREQTHESLRGALLEECYEAI
                     EAINNADDANLREELGDLLLLVVMHAQMGAERGAFAFEEIAAAVAEKMIRRHPHVFGD
                     QTAADSAAVLKQWEQIKRREKGENASVLAGVARALPALMRAQNVQKKAARVGFDWGSA
                     IEVLDKLEEEIRELREAMASGQPAAIGEEAGDLLFSAVNLARKLGLESETVLNESTDK
                     FVRRFQALEQTVAAAGKRVEDVSLEELEAHWQRAKRA"
     gene            1938..>2289
                     /locus_tag="VIS74_05820"
     CDS             1938..>2289
                     /locus_tag="VIS74_05820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792796.1"
                     /translation="MIPFAVLNPGGSDPEQLFSHGAGHPGDPGHPPVNYHAYAACLNG
                     GFYRDVRRLPKPAGVVVILLRNRIHFALDALKSARAAGWKTWITFKETGAHQLAEFLE
                     DPKRVEALRRLCGEA"
BASE COUNT          439 a          677 c          786 g          387 t
ORIGIN      
        1 caggtgctgg ccgtggcggt tgatggccag cacgacaatg ccctgccgcg cggggaggtc
       61 gagatccatg atccgccgcc cgcgcagcgt cgaatgcgga gtgacgatga gttcgaggat
      121 ttgctgccgg tcgctggtca gcgccttgtc gccaaattta aaatcggcct tgatctcgat
      181 gccctcggtt tcccggacct tcatgaggct ttccgccgcg ccgttgacga gcaggagatc
      241 gcccgccgcg agcttcagcc gcgggtgcgg cgcgagggac ttgccttcgc gaatcacccg
      301 cagcacgcgg aagtccattt tcccgagatc ggaatggctg agcggctggc cggcgagcgg
      361 ggagttttcc cggatcagga tttccgagag gtattcgcgc aggccgtagt cctcggtgag
      421 ctcctggacc ggggcgtgat cgggcagcag cttctggccg aagaaccgca tgtagagaat
      481 gccgatcgcc acaatcagaa ggccgatcgg cgtgagttcg aaaaagccga tgggccgttc
      541 gccgctttgg gtcaggaaac cgctgacggc gacattcgtg gacgtgccga tgagcgtgca
      601 ggtgccgccg aggatggacg caaacgccat gggcatgagc agcttcgacg cgctgatttt
      661 ccgccgccgc gccatgccga tgaccggcgt gagcatcaat gcggtgaccg tggtattgtt
      721 catgaacgcg gaaaggccgg agacgctgcc catcatgagc gtgagaaagg ggttcttccc
      781 ccctttcgcg aagcgcgaaa gggcgtcgcc gacccagtcc accacgccgg tttcccgcat
      841 ggcgccgctg atcacgaaga ccgagccgag aatgagaatg atgtcgctgc caaagcccgc
      901 gaaggcttcc tgcggcgtca ggatgcccgt gaagagcagc gccagcagca tgaggaaggt
      961 gatgagatcg accgacagct tttcggtcgc aaagaggatc attgcgatca cgagcaggct
     1021 gagaacgaag gcgatggtca tccggcgaga tgcctctttt cttgcggcgg cgcccggcga
     1081 agcacaaggc aaattgaaca aatactccac aacgggcgtt gccgggagag gaaactcgac
     1141 ttgccgggga cgtttggctt aatggggggc atcatgagtg aagccgtcgg cagattgacc
     1201 gaaatcgtgc gcaccctgcg gtctcccgaa ggctgcccct gggaccgcga gcagacgcac
     1261 gaaagcctgc ggggcgccct gttggaggag tgctacgagg ccattgaagc catcaataac
     1321 gccgacgacg cgaacctccg cgaggaactc ggcgacctgc tcctgctcgt ggtgatgcac
     1381 gcccagatgg gcgccgagcg gggcgccttc gccttcgagg aaatcgccgc cgccgtcgcg
     1441 gaaaaaatga tccggcggca tccgcacgtg tttggcgatc agaccgccgc cgattccgcc
     1501 gccgtcctca agcaatggga gcagatcaag cgccgggaaa agggggagaa cgcctccgtg
     1561 ctggccgggg tggcgcgggc gcttccggcc ctgatgcgcg ctcagaacgt gcagaaaaaa
     1621 gccgcgcggg tcgggttcga ctggggcagc gccattgaag tgctcgacaa gctggaggag
     1681 gaaatccgcg aattgcgcga ggcgatggcc tccggccagc cggccgccat cggcgaggag
     1741 gccggggatt tgctgttttc cgcggtgaat cttgcccgga agctgggttt ggaatcggag
     1801 accgtcttga atgaatccac cgacaagttc gtgcggcggt ttcaggccct cgaacaaacc
     1861 gtcgccgcgg cgggcaagcg ggtggaggac gtttccttgg aggaattgga ggcgcattgg
     1921 caacgggcca agcgggcatg attccctttg cggttttgaa ccccggaggc agcgatccgg
     1981 agcagctctt ttcccacggc gcgggccatc ccggggatcc cggccatccg ccggtgaact
     2041 atcacgccta cgcggcgtgc ctgaacggcg ggttttatcg cgacgtccgg cggctgccca
     2101 agccggcggg agtggtggtg attttactgc gcaatcgaat ccatttcgcc ctggacgccc
     2161 tcaagtccgc gcgcgcggcg ggctggaaaa cctggatcac gttcaaggaa accggggcgc
     2221 atcaactcgc ggaatttctg gaagacccca agcgggtcga ggccctgcgc cggctctgcg
     2281 gcgaggcgg
//
LOCUS       DATDAY010000288         2500 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_993936, whole genome shotgun sequence.
ACCESSION   DATDAY010000288 DATDAY010000000
VERSION     DATDAY010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2500)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2500
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_993936"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..334
                     /locus_tag="VIS74_05825"
     CDS             <1..334
                     /locus_tag="VIS74_05825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792797.1"
                     /translation="VVLIAPSPRAWAFRSQVGQLQQVYERLAAMKAVCIAAFTEENGR
                     IRSNIPFAGVPDGPRVAYDYNVQRGFAIGVIGRDGNLDYIDNRVTSGQRVLDIIQNSF
                     VLQAQLRR"
     gene            340..1449
                     /locus_tag="VIS74_05830"
     CDS             340..1449
                     /locus_tag="VIS74_05830"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HEY5792798.1"
                     /translation="MRYLVFKPDGIGDFVLASGGYRLLADLAGEENLTLVVMPPVKSI
                     ARSQFPKATVIALPMEKKRKVVNVFVMNFLRCLGPWREIRRHPMEAAISFRTMRAYLH
                     TFLFYSTRTKHFFGCENLLIRNRRWSRRFVEWVTRTFLAPRIAPYPEAAEGVPSELEA
                     HRRVLSLALNREVALAEILPRLRTETATVDGNYWLCSPLSAIPSKNYPLESWCEVFRQ
                     VRDLIGGSEIRLTGSADQKSLLDELAGRLRAEQFSAIVELRPTLQGFVELINEAKWVL
                     TIDTAAAHMACALDKRCLILFSGLLRGMFGPWTRSPRQVWLDPLPPPDGIKRKWFRGM
                     PPDRVSAAIRALNEIGGVTPSARRNPATSGNAGFP"
     gene            complement(1394..>2500)
                     /locus_tag="VIS74_05835"
     CDS             complement(1394..>2500)
                     /locus_tag="VIS74_05835"
                     /inference="COORDINATES: protein motif:HMM:NF012312.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY5792799.1"
                     /translation="IYAPVQLVVGWSLDRFGAKRVLVPAILLCAAGCFLEAFGITAWM
                     LTSARLLQGFGSAFAFVGAMYLATKWFPANKLALLAGLTTALGMLGAIGVNAGLVEIV
                     QDVGWRSTLWGAGIAGIGVAALVFWIVPHDKPRNAEGLGEAVEEEVHSLWKSLRVVGG
                     NPQTWLAAVVATALYMPLTLLGALWGVEYVATVTQEGKVQASGAISMIYVGWLIFGPF
                     TGWLSDRWGRRKLLVLASSVLTLLASGLLLLFTNVPLWAVYGLMLLIGIASSPQTVSF
                     VMAVEHNPRSMSGTAIAVVNMLVMLLGGLCMPLFGCLLELATPGHGEVYSPTAYRLAM
                     ILLPASMMLSILACVFLKESRHCRKLPDYAEPMG"
BASE COUNT          473 a          871 c          754 g          402 t
ORIGIN      
        1 cgtggtgctc atcgccccct cgccccgcgc ctgggccttc cgttcgcagg tgggacagct
       61 gcagcaggtt tacgaacgcc ttgccgcgat gaaggcggtt tgcatcgccg ccttcacgga
      121 ggagaacggc cgcatccgct cgaacattcc ctttgccggg gtgccggacg gcccgcgagt
      181 ggcctatgac tacaatgtgc agcgaggttt cgccatcggc gtgatcgggc gcgacggaaa
      241 cctggattac atcgacaacc gcgtgacgag cggacagcgg gttctcgaca tcatccagaa
      301 ttccttcgtg ctgcaggcac aattgcggcg ctgaccggcg tgcgctacct cgtcttcaag
      361 ccggacggca tcggcgactt cgtcctcgcc tccggcggct accgcctgct cgcggacctg
      421 gcgggggagg agaatctcac ccttgtggtc atgccgccgg tcaaatccat cgcgcggtcc
      481 cagtttccca aggccacggt catcgcgctg cccatggaaa agaagcggaa agtcgtgaat
      541 gtctttgtca tgaatttcct gcgctgcctg gggccgtggc gggaaatccg gcggcacccc
      601 atggaggccg ccatctcctt ccgcaccatg cgggcgtatc tgcatacctt cctgttttat
      661 tccacccgga cgaaacattt tttcggctgc gaaaatctgc tcatccgcaa ccggcgctgg
      721 agccggcgct tcgtggaatg ggtgacgcgc accttcttgg ctccccggat cgccccgtat
      781 ccggaggcgg ccgaaggcgt tccctcggag ctggaggcgc accgccgggt gctgagcctc
      841 gcgctgaacc gcgaggtggc cctcgcggaa atcctgccgc gcctgcggac cgaaaccgcc
      901 acggtggatg gaaactactg gctctgcagc ccgctgagcg cgatcccctc caagaactat
      961 ccgttggaaa gctggtgcga ggttttccgc caggttcggg atttgatcgg cgggagtgaa
     1021 atccgcctga ccggttccgc cgaccaaaaa agcctgctgg acgagctcgc cgggcggttg
     1081 cgggcggaac aattttcggc catcgtcgaa ctccgcccca cgctgcaggg cttcgtcgag
     1141 ctgatcaacg aggccaaatg ggtgctcacc attgacaccg ccgccgccca catggcctgc
     1201 gcgctggaca agcgctgcct cattctcttc tccggcctgc tgcgcggcat gttcggcccc
     1261 tggacccgga gcccccgcca ggtctggctc gatccgttgc cgccgccgga cgggatcaag
     1321 cgcaaatggt tccgcggcat gccgccggac cgggtgagcg cggccatccg ggcgctgaac
     1381 gaaatcggag gcgttacccc atcggctcgg cgtaatccgg caacttccgg caatgccggc
     1441 tttccttgag aaaaacgcag gccaggatgc tcagcatcat gctcgccggc agcaggatca
     1501 tcgccaggcg ataagccgtc ggcgaataca cctcgccatg ccccggggtg gccagttcga
     1561 gcaggcagcc aaagagcggc atgcaaagtc cgcccagcag catcaccagc atgttgacga
     1621 ccgcaatggc cgtgccgctc atggagcggg gattatgctc caccgccatg acaaatgaaa
     1681 ccgtctgggg gctggaggcg atgccgatga gcagcatcaa cccatacact gcccagagcg
     1741 gaacattggt gaaaagcagc agcaagcccg aagccagcaa cgtcagcacg gacgacgcga
     1801 gaaccagcag tttgcggcgc ccccacctgt ccgacagcca gcccgtgaat gggccgaaga
     1861 tcagccagcc gacatagatc atcgaaatgg cgcccgaggc ctgcaccttg ccctcctgcg
     1921 tgaccgtggc cacgtattcg acgccccaca atgcccccag cagggtcagc ggcatgtaaa
     1981 gcgccgtggc caccaccgcc gccagccagg tctgcgggtt tccgccgacg acccgcaggg
     2041 atttccacag ggaatgcact tcctcctcca ccgcctcgcc gaggccttcc gcgttccgcg
     2101 gcttgtcgtg cggcacgatc caaaatacca gcgccgccac cccgatgccc gcaatgcccg
     2161 ccccccacag cgtcgaacgc cagcccacgt cctggacgat ttccaccagc cccgcgttca
     2221 cgccgatggc gcccagcatg cccaaagcgg tggtcagccc ggccagcagg gccagcttgt
     2281 tggcgggaaa ccatttggtg gcgagataca tggcgcccac gaaggcgaac gccgacccaa
     2341 acccctggag cagccgggcc gaggtcagca tccacgccgt gatgccaaac gcctccagga
     2401 aacaccccgc cgcgcagagc aggatggccg ggaccagcac ccgtttcgcc ccaaaccgat
     2461 ccagcgacca gcccaccacg agctgaaccg gggcgtaaat
//
LOCUS       DATDAY010000289         2075 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_801715, whole genome shotgun sequence.
ACCESSION   DATDAY010000289 DATDAY010000000
VERSION     DATDAY010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2075)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2075)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2075
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_801715"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            19..267
                     /locus_tag="VIS74_05840"
     CDS             19..267
                     /locus_tag="VIS74_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIL domain-containing protein"
                     /protein_id="HEY5792800.1"
                     /translation="MSQQVTRLWLMYPQKLITRPVLHELGHKFTVITNIRQASVNNEI
                     GVVSIELDGEREEIKRAIQWLEEIGIKVEPVEINTIEG"
     gene            383..1387
                     /locus_tag="VIS74_05845"
     CDS             383..1387
                     /locus_tag="VIS74_05845"
                     /inference="COORDINATES: protein motif:HMM:NF017280.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HEY5792801.1"
                     /translation="MLAPDQIAITDFGPYLAKYPFDPTNGFSLAELRQLRVAEKAPPD
                     FVDFWQNAHERSRAVDPRAEFRPSGFAAPDHDLRELHFHSLGGFAIGAWLAEPRDRNR
                     IRRVEIFGHGYGGRSEPDRAALAEEAVQLFVCAPGFDLSACPDRVATNAAMLHVLTGI
                     HSPETYILLACAAAVWTAARVMDDLFPGLPQVYRGGSFGGGIGALLLPWESRFVAAEL
                     VQPTFGYHTLRLRHRSTGSGESVRRLHELQPSIRRTLAYFDAAYAARHLKVPVAVAAC
                     AFDPAVCPPGQFAVANAIPGDLRRVSEFSFGHCDFPHPDRAFEDQRHREQLAAILAKI
                     "
     gene            complement(1431..1691)
                     /locus_tag="VIS74_05850"
     CDS             complement(1431..1691)
                     /locus_tag="VIS74_05850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792802.1"
                     /translation="MNSIALEELDLAIPNLESHHDEIRAGEILKGIPGINAVRFIERG
                     AWISYQATQITRQQICDLLHRSGFRASTFQDSKSGQTGVSSV"
     gene            1877..2059
                     /locus_tag="VIS74_05855"
     CDS             1877..2059
                     /locus_tag="VIS74_05855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792803.1"
                     /translation="MRKRIWRNLKYPLLIGRERTLILVALILVIVIGVIFAGLMTRKA
                     EQARLKETTEMATPFR"
BASE COUNT          381 a          639 c          641 g          414 t
ORIGIN      
        1 cggcggtaaa tttcccgcat gtcccagcaa gttacccgtc tttggttgat gtatccgcag
       61 aagctcatca cgcgccccgt gctgcatgag ctcggccaca agttcacggt catcacgaac
      121 atccgccagg ccagcgtaaa caacgaaatc ggcgtggttt cgatcgaact cgacggcgag
      181 cgcgaggaga tcaagcgcgc catccagtgg ctcgaagaga tcggcatcaa ggtcgagccc
      241 gtcgagatca atacaatcga aggttaaccg cccggggcgt accggtctga tcaccggcaa
      301 gccgggttcg gcggcccgcc ggttttcagg cggcccggaa atctccacgt gaatttcgct
      361 gccgcgcggt tagagtgcgt tcatgcttgc gccggatcaa atcgcgatca cggactttgg
      421 cccctatctc gccaaatatc ccttcgaccc gaccaacggc ttttcgctgg cggaactccg
      481 ccagctccgc gtcgcggaaa aagcgccgcc ggatttcgtg gatttctggc agaacgcgca
      541 cgagcgcagc cgggccgttg atccgcgggc ggaattccgg ccctcgggtt ttgcggctcc
      601 ggatcacgac ctgcgcgaac tccattttca cagcctcgga ggttttgcca tcggcgcctg
      661 gctggcggag ccgcgggacc ggaaccgaat ccggcgggtg gaaattttcg gccacggcta
      721 cggcgggcgt tcggaaccgg accgggccgc gctggccgaa gaggcggttc aattgtttgt
      781 ctgcgcgccg ggattcgacc tttccgcctg ccccgaccgc gtggcgacca atgcggcgat
      841 gctgcatgtc ctcaccggca ttcattcgcc ggaaacctac atcctgctgg cctgcgcggc
      901 ggcggtctgg acggcggcgc gggtgatgga cgatctcttc cccgggcttc cgcaggtgta
      961 tcgcggcggg agttttggcg ggggaatcgg cgcgctgctc ctgccgtggg aatcccgctt
     1021 cgtggccgcg gagctggtgc agccgacctt tggttatcac accctccgcc tgcggcaccg
     1081 cagcaccggc agcggcgaga gcgtgcggcg gcttcacgag ctgcagccct cgatccggcg
     1141 cacgctggcg tattttgacg cggcctacgc cgcccggcac ctcaaggttc cagtcgccgt
     1201 ggcggcctgc gcctttgatc ccgcggtctg cccgcccggg caattcgccg tggcgaacgc
     1261 gatccccgga gacctgcggc gcgtctcgga attctcgttc ggccattgcg attttccgca
     1321 cccggaccgg gcattcgagg accagcgcca ccgcgagcaa ctcgcggcga tcctggcgaa
     1381 aatttaggcc gatcccggcg cggcgccggg gccgcggcgg ttaccggttc tcagaccgag
     1441 gaaaccccgg tttggccgga tttcgaatcc tgaaacgtgc tggcccggaa accggagcgg
     1501 tgcagcagat cgcagatttg ctggcgggtg atttgcgtgg cctgatagga aatccaggcg
     1561 ccgcgttcga tgaaacgcac ggcattgatg cccggaatgc ctttgaggat ttcaccagct
     1621 ctgatttcat catgatgcga ttccagattg ggaatcgcca gatccaactc ttccaatgcg
     1681 attgaattca tacgacgagt ctgtgattat tattctagaa gccccttggg aaaacacaac
     1741 cgcaagttgc gtgggcggag aaagcatcgc cttacagtag tgcacgcttt ggtttttttg
     1801 aaaaagcgcc cgccccttgt gaatccatcc aaccagcgta ggtttgtcca tggaagaatg
     1861 ggggcataaa atccggttgc gaaaacgaat ctggcgcaac ctgaagtatc cgttattgat
     1921 tggccgggaa cgcaccctga ttctggttgc cttgatcctg gtaatcgtga tcggcgtgat
     1981 ttttgccggc ctgatgactc gcaaggcgga acaggcgcgc ctgaaagaga ctacggaaat
     2041 ggcgactccc ttccgctgat ctccctgaac tgaaa
//
LOCUS       DATDAY010000290         2016 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_979839, whole genome shotgun sequence.
ACCESSION   DATDAY010000290 DATDAY010000000
VERSION     DATDAY010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2016
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_979839"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..541)
                     /locus_tag="VIS74_05860"
     CDS             complement(<1..541)
                     /locus_tag="VIS74_05860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaL"
                     /protein_id="HEY5792804.1"
                     /translation="MDKPLVASYCSIFLKREMRHLYRQVTGLRRYRTCVLAQERINGD
                     SYPFPDIELLKKPRPHPFRHAARKFRGEPPLYYRGEADVLASVLRRRSPVLMHVYFGH
                     IGVHLLPFIQRWPHPTIVSFHGMDAMPRNENPAYADKMRELLETVPLALARSESLARV
                     LEERGAPKNRIRINRTALPL"
     gene            666..1247
                     /locus_tag="VIS74_05865"
     CDS             666..1247
                     /locus_tag="VIS74_05865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEY5792805.1"
                     /translation="MPSAESSDPAPDDWELMRRIKGEDMAAFRALVERHQHSVVGVAA
                     KMLGDFTDAEDLAQQVFLRIWKSAGRYEPSAKFTTWLMTITRNLVFNEMRRRARNRQV
                     PLEGEGAAREFADDPNKQPAQSAAAAELEAAIDEAITGLPEPQRIALVLRRYEEMPYE
                     EIARVLKVSVPALKSLLFRARETLKVRLKRYLE"
     gene            1300..1914
                     /locus_tag="VIS74_05870"
     CDS             1300..1914
                     /locus_tag="VIS74_05870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792806.1"
                     /translation="MPKEENVLVIKRSLFEELGAFQGFCAEAPRYLEKFLKRENNYFL
                     PRSRAEEDPTHKQLIPYVVFTHANRILRYVRGAKSGEKRLVAKGSIGIGGHINDEDEG
                     LFAFDDQAYAEAVKREISEELRLPGGWTLRLAGLINDDGNEVGRVHLGVVHVCELPSG
                     EVGAGEKAIAKLEFLTADELRAEEDALETWSQIVFRNWPVISAN"
BASE COUNT          404 a          532 c          693 g          387 t
ORIGIN      
        1 tcaacggcag cgcggtgcgg ttgatccgga tgcggttttt tggcgcgccg cgttcctcga
       61 gcacgcgcgc cagcgattcg ctgcgggcga gcgcgagcgg gaccgtttcg agcagctccc
      121 gcattttgtc cgcgtaggcg ggattttcgt tccgaggcat ggcgtccatg ccatgaaagg
      181 acacgatcgt cggatgcggc catcgctgga tgaacggcaa taagtgaacg ccgatgtgcc
      241 cgaaatagac gtgcatcaag accggcgagc gccgccgcaa tacggaggcc aggacgtccg
      301 cctcgccgcg gtaatacagc ggcggctcgc cgcggaattt gcgggcggcg tggcggaacg
      361 gatggggccg aggtttcttc aaaagctcga tatcggggaa aggatagctg tcgccattga
      421 tccgctcctg ggcgagcacg caggtgcggt aacgccgcag tccggtgacc tggcggtaga
      481 gatgccgcat ttcccgcttg aggaaaatgc tgcaatagga ggcaaccagc ggtttgtcca
      541 tgggccgccc aagatgaggg aggaagggcg gatggcgcga gtttttttgc gaatcttttc
      601 ggagcgcgcg gggttaaaat tcctgcatga cggcatccct gacttgcggc agtattttcc
      661 aacctatgcc ttccgccgaa tcgtccgatc ccgcgccgga tgactgggag ttgatgcgcc
      721 ggatcaaggg ggaagatatg gcggcctttc gtgcgctggt ggaacggcat cagcacagcg
      781 tggtcggggt ggcggcaaaa atgctgggcg actttaccga tgcggaggat ttggcgcagc
      841 aggtgtttct gcgaatctgg aaatcggccg gacgctacga gccgtcggcc aagtttacga
      901 cctggctgat gaccatcacg cggaatctgg tttttaatga aatgcgccgc cgcgcccgca
      961 atcgccaggt tcccctggag ggcgaaggcg ccgcccggga atttgcggat gacccaaaca
     1021 aacagcccgc gcaatccgcc gcggcggctg aactggaggc ggcgatcgac gaggcgatca
     1081 ccggcctgcc cgaaccgcag cgcatcgcgc tcgtgctgcg ccgttacgaa gagatgccct
     1141 acgaggagat cgcccgggtg ctgaaggtga gcgtgcccgc gttaaaaagc ctgctgtttc
     1201 gcgcccggga gacgctgaaa gtccggctga agcggtattt ggaataaggc ggccattttt
     1261 cgctgtgtga ctggggcggg ctttgattaa tctccgcgaa tgccgaagga agaaaatgtc
     1321 ttggtcatca aacgcagcct cttcgaagag ctgggagcct ttcagggatt ctgtgcggag
     1381 gcgccgcggt atctggaaaa atttttgaag cgggaaaaca attatttcct gccgcgttcg
     1441 cgggcggagg aggatccgac gcacaagcaa ttgattcctt atgtggtttt cacccacgcg
     1501 aaccgcattc ttcgctacgt gcgcggggcg aagtccggcg aaaagcgctt ggtggcgaag
     1561 ggttccatcg gcatcggcgg ccacatcaac gacgaggacg agggtctttt tgcatttgac
     1621 gatcaggcgt atgcggaggc ggtcaaacgc gaaatctccg aggagctccg gctgcccggc
     1681 ggttggacgc tgcggctggc gggcctgatc aacgatgacg gcaacgaggt cggcagggtg
     1741 cacctgggcg tggtgcacgt ctgcgagctg ccgagtggcg aagtgggcgc gggggagaag
     1801 gcgatcgcca aactcgaatt tttgacggcg gacgaactgc gggccgagga ggacgcgctc
     1861 gagacctggt cgcagatcgt gttccgcaac tggccggtga tttccgcgaa ttgagcaaag
     1921 cgagattggc cggatttccg cagtgagctg gaaataaaaa aagcggtctc attgctgaga
     1981 ccgctttggg tttgagattg gaaaactcgg attagc
//
LOCUS       DATDAY010000291         2379 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_432560, whole genome shotgun sequence.
ACCESSION   DATDAY010000291 DATDAY010000000
VERSION     DATDAY010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2379)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2379)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2379
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_432560"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..387)
                     /locus_tag="VIS74_05875"
     CDS             complement(<1..387)
                     /locus_tag="VIS74_05875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HEY5792807.1"
                     /translation="MASPKIDPELHQAKKPEWIKVRLPSNPVFFSTKALVADLKLHTV
                     CESAQCPNRWECWSQGTATFMIAGERCTRACGFCAVSTAKPFDLEADEPQRVAEAVRR
                     LKLKHVVITAVARDDLADGGAEHFAQT"
     gene            complement(520..1134)
                     /gene="recR"
                     /locus_tag="VIS74_05880"
     CDS             complement(520..1134)
                     /gene="recR"
                     /locus_tag="VIS74_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HEY5792808.1"
                     /translation="MSGISRASYPEPLEVLLRELRRLPGIGPRSAERIGLWLVTSADA
                     RPNELAAALTEMKTAVRACRLCGFFSLEEVCPLCADPNRAHKELCVVERATDILPIEK
                     TNLYHGRYHALGGRLSPLEQIGPEELRIGILIERIQAEPPAEIILALSADVEGEATTA
                     YLSQELAPLGVPVTRIAHGLPAGGGLEHADPLTLLRAFTGRKPL"
     gene            complement(1103..>2379)
                     /locus_tag="VIS74_05885"
     CDS             complement(1103..>2379)
                     /locus_tag="VIS74_05885"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEY5792809.1"
                     /translation="SKQPRRRPGLTFNPMKSVFTALLGAALAIGLLLLLKGIGLVDWL
                     NGVIRTAAGMPGEGAFISLSGQLLIACFAALWLAWVVVDMPSSRRKWLIMAGVIFLTL
                     TGAGVWALYGVFFSPAVPILGALFSTTVTYVFCLIGPGLLKNRLEQIFGLSISRKHLF
                     SLYERNAADVLVPHRVSVTVAVLEVDNHHAILESLPATEYAALTSFYFSAAADFLADS
                     GGFLDECGGQNLRVIFGAPVANENSAAAACRALLQLKRRLDRLGAEADERWHRALEFR
                     IGAATGEVVAGVFGANRNPVFSIAGPTVDHAHRLCDLCEHYGSHFLIGSETNQKAAES
                     LESRPVDFVALQGHETSEMFEVLGAKNSLSPERTRSRDHYWKGVIYAREQRWDAAVEE
                     FSRARVKGIPDPLINFYLQRIERERNLPSVLS"
BASE COUNT          452 a          745 c          796 g          386 t
ORIGIN      
        1 ggtctgcgcg aaatgctcgg cgccgccgtc cgcgagatcg tcgcgcgcga cggcggtgat
       61 gacgacatgc ttcaatttca gccgccggac ggcctcggcc acccgctgcg gctcgtcggc
      121 ttcgagatcg aacggcttgg cggtggacac ggcgcagaat ccgcaggcgc gcgtgcagcg
      181 ttcgccggcg atcatgaacg tcgcggttcc ctggctccag cactcccagc ggttcgggca
      241 ctgggcgctt tcgcagaccg tgtggagctt gaggtccgcc accagcgcct tcgtggaaaa
      301 gaacacggga ttcgaaggca ggcggacttt gatccattca ggtttcttgg cctgatgaag
      361 ttcgggatca atcttgggtg acgccatttg gaaaaagaaa ccgctttgcc gccggaaagc
      421 aagtcaactt ccgggggtga accacagagg acacagagat cacagaggga aaaccgaatt
      481 aaatttctct gcggcctttg tgtcctctgt ggttaaactt cacagcggct tccgcccggt
      541 aaaggcgcgc aagagggtca atgggtcggc atgctccaaa ccgccgccgg ccgggaggcc
      601 gtgggcgatc cgggtcaccg gcaccccgag cggcgccagt tcctgggaga gatacgcggt
      661 cgtcgcctcg ccctccacgt cggcgctgag cgcgagaatg atttccgcgg gcggctccgc
      721 ctggatgcgc tcgatcagaa tgccgatccg caactcctcc gggccgatct gttccagcgg
      781 ggaaagccgt ccgccgaggg cgtgatacct cccgtggtag agattcgttt tttcaatggg
      841 caggatgtcc gtcgcccgct ccaccacgca gagttccttg tgcgcgcggt tgggatcggc
      901 gcacaggggg cagacttcct ccaggctgaa aaacccgcag agccggcagg cccgcacggc
      961 ggttttcatt tcggtcaaag ccgccgcgag ctcgttcggg cgggcatccg cggaggtcac
     1021 cagccagagg ccgatccgtt ccgcgctcct tggaccgatg ccgggcagcc ggcgcagctc
     1081 gcgcagcagg acctccagcg gctcaggata ggacgctcgg gagattccgc tcacgttcga
     1141 tgcgttggag ataaaaattg atcagcggat cggggatgcc cttgacgcgc gcgcgggaaa
     1201 attcctccac ggcggcatcc cagcgctgct cgcgggcgta gatcacgccc ttccagtaat
     1261 gatcccggct gcgggtgcgc tccggcgaga gggaattctt cgcgccgagg acctcgaaca
     1321 tttccgaggt ctcgtgcccc tgcagcgcca cgaagtccac ggggcgggac tcgagggatt
     1381 ccgccgcttt ttgattggtc tcggaaccga tgagaaaatg gctgccgtag tgctcgcaca
     1441 ggtcgcagag ccggtgcgca tgatccaccg tggggccggc gattgagaag accggattgc
     1501 gattggctcc aaaaaccccg gcgacgactt cgcccgtggc cgcgccgatg cggaactcca
     1561 gcgcgcggtg ccagcgctcg tcggcctccg cgccgagccg gtcgagccgc cgcttcagct
     1621 gcagcagcgc ccggcacgcc gccgcggcgg aattttcatt cgccaccggc gcgccgaaga
     1681 tcacccgcag gttctggccg ccgcattcgt cgagaaaacc gccggaatcc gccaggaagt
     1741 cggcggcggc gctgaaataa aaactcgtca gcgccgcata ctcggtggcc ggcaggcttt
     1801 cgaggatcgc gtgatgattg tccacctcca gcaccgcgac ggtcaccgaa acccggtggg
     1861 gaaccagcac gtcggccgca ttgcgttcgt agagcgagaa aaggtgcttg cgcgaaatgc
     1921 tcaggccgaa aatctgttcg agccggttct tgagcaggcc gggcccgatc aggcagaaga
     1981 cgtaggtgac cgtcgtgctg aaaagcgcgc cgaggatggg caccgcgggg gaaaaaaaca
     2041 cgccgtaaag cgcccacacg ccggcgccgg tgagagtcag gaagatcacc ccggccatga
     2101 tgagccactt gcggcgcgag gacggcatgt ccacgacgac ccaggccagc cagagggcgg
     2161 caaagcaggc gatgagcaat tggccggaga gactgatgaa ggcgccctcg cccggcatgc
     2221 ccgccgcggt gcggatgacg ccattgagcc agtccaccag gccgatccct ttcagcagaa
     2281 gcagcagacc gatggcaagc gcggcgccca gcagggcggt aaaaacggat ttcatggggt
     2341 tgaaggtgag gccgggccgc cggcggggtt gtttcgata
//
LOCUS       DATDAY010000292         2511 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_99982, whole genome shotgun sequence.
ACCESSION   DATDAY010000292 DATDAY010000000
VERSION     DATDAY010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2511)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2511)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2511
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_99982"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1431)
                     /locus_tag="VIS74_05890"
     CDS             complement(<1..1431)
                     /locus_tag="VIS74_05890"
                     /inference="COORDINATES: protein motif:HMM:NF019583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase domain-containing protein"
                     /protein_id="HEY5792810.1"
                     /translation="MDSIADWVDPLIGTDSTRDFSSGNVLPLVSLPFGMTGWIPQSDT
                     SPWLYQYRQRKLGGFRATHQPSPWMGDYGHFTLMPQTGPLELAFSNRASVYRHDQTTI
                     KPFLLRTWLDRYEIGVSLAATERGAVFYFDFPRQGKSRLIFDLPIHTHLAYDPARRRL
                     EFSLHNHSGGAPHHFGFHAVLQFKGIRKTEFRVIGEQAVLEFVDKVDRAEVRIGTSFI
                     SREQAAFHLEREIGDKKLPQIANLGRAAWNNLLGRVTLGGADEAQKRTFYTALYRTLI
                     FPRKFHEPTPKNGLQHFSPFDGKVHDGVLYTDTGFWDTYRNVYPLFAFLYPDLLGEII
                     QGWINAFLEGGWFPKWASPGYRICMIGTHIDAVIACAVSRGIDGFDLGDAYEGIRKNA
                     FEAGDPKGAWGREGLKDYLKLGYLPADRVDHSCAATQEYAFSDFCIAQVARLVGQDRD
                     HEELLRRALNYRHVFDPRAGFMRGRNA"
     gene            complement(1772..2332)
                     /locus_tag="VIS74_05895"
     CDS             complement(1772..2332)
                     /locus_tag="VIS74_05895"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY5792811.1"
                     /translation="MTQPTLRTDRLILRPFISGDAAAVQELAGEFAIADTTLNVPHPY
                     ADGLAESWIAAHEAQYRAGEAATFAITLKADLALIGAIGLTLFPRFRRGELGYWIGVP
                     FWNRGFCTEAVRAVIDFGFTTLKLHKICAHHLSRNPASGRVMQKAGMSKEGTFKDQTI
                     KWDRFESVDAYGRIVTDTLSLAAVEP"
BASE COUNT          507 a          748 c          799 g          457 t
ORIGIN      
        1 ggcgttgcgc ccgcgcatga acccggcgcg gggatcgaag acgtggcggt agttgagcgc
       61 gcgccgcagg agttcctcgt gatcccggtc ctggccgacg aggcgggcga cctgggcgat
      121 gcaaaaatcc gagaacgcgt attcctgggt ggccgcgcag gaatggtcca cccggtcggc
      181 cggcaaataa cccagcttga ggtaatcctt caatccctcg cggccccagg cgcctttggg
      241 gtctccggcc tcgaaggcgt ttttgcggat gccttcgtag gcgtcgccga gatcgaaccc
      301 gtcaattccc cgggaaacgg cgcaggcgat gacggcgtcg atgtgcgtgc cgatcatgca
      361 gattcgataa ccgggactcg cccatttggg aaaccagcct ccctccaaaa aggcgttgat
      421 ccacccctga atgatctccc cgagcagatc gggatacaga aaggcgaaca gcggataaac
      481 gttgcgatac gtgtcccaaa aaccggtgtc ggtataaagc actccgtcat gcaccttccc
      541 gtcgaacgga ctgaagtgct ggaggccgtt cttgggagtg ggctcatgga atttgcgcgg
      601 aaaaatcagg gttcggtaga gcgcggtata gaaggtgcgc ttttgcgcct cgtccgcccc
      661 gcccagggtg acgcgcccga gaaggttgtt ccacgcggcc cggcccaggt tggcgatctg
      721 cggcagtttt ttatccccaa tttccctttc caggtgaaac gccgcctgct cccggctgat
      781 gaacgaagtg ccgatccgca cctccgcgcg atccaccttg tccacaaact ccaggaccgc
      841 ctgctccccg attacccgaa actcggtttt gcggatgccc ttgaattgca gcacggcgtg
      901 aaacccgaag tggtggggcg cgccgccgct gtggttgtgc aggctgaatt ccagccgccg
      961 ccgcgcgggg tcgtaggcca gatgcgtgtg aatgggcaga tcgaaaatca ggcgggattt
     1021 cccctggcgg gggaagtcga aataaaagac ggcgccgcgt tccgtagccg ccagcgagac
     1081 gccgatctcg taacgatcca gccacgtccg cagcaggaag ggcttgatcg tggtttgatc
     1141 gtgccggtaa acggaagccc ggttggaaaa ggccagctcc agcgggccgg tttgcggcat
     1201 cagggtgaaa tgcccgtaat cgcccatcca ggggctcggc tgatgggtgg cccggaagcc
     1261 gcccaatttg cgttgccggt attgataaag ccagggggag gtgtcggatt gcggaatcca
     1321 gcccgtcatc ccaaacggca gggaaaccag gggcagcacg tttcccgagg aaaagtcccg
     1381 ggtggagtcg gtgccgatca gcggatcgac ccagtcagca attgaatcca cgagagacat
     1441 gaacggttcc tttccccata gcgtgcattg aaagtcggcg agagtaaaaa gaaaggtgca
     1501 attgaaaagg agaaaagaag gaggaaggga aggaatgccg aaaaccggga gggaaacact
     1561 ccctcgttcc ccctggcccc gcaacccgct tttccgccga gcgggtgaga ggaagccgcg
     1621 cgaatccttt tttcgtggag ggcggcggtc cctcgccgcc gtggcttatc gctggacgtt
     1681 cgatccgttc gccatccctt ttttgaaggg tttcgctcgc gtcctgcatc ccgcgacagc
     1741 ggcgagagcc cgccgccctc caaatcacgg gctacggctc gacggccgcg aggctcagcg
     1801 tgtcggtcac gattcgcccg taagcatcca cgctctcaaa tcgatcccac ttgatcgtct
     1861 gatccttgaa ggtgccttcc ttgctcatgc cggccttttg catgaccctt cccgaggcgg
     1921 gattgcggga gagatgatgc gcgcaaatct tgtgaagctt cagcgtcgtg aatccaaaat
     1981 cgatcaccgc ccggacggct tcggtgcaaa aaccgcgatt ccaaaacgga acgccgatcc
     2041 agtagcccag ctcgccccgg cggaagcggg gaaaaagagt caggccgatc gccccgatca
     2101 aggcaaggtc ggctttgagc gtgattgcga aagtggcggc ttcgcccgcg cggtattggg
     2161 cctcatgcgc cgcgatccag ctttccgcca ggccgtccgc gtagggatgc gggacattta
     2221 gcgtggtatc cgcaatcgcg aattcgcccg ccagttcttg gacggcggcg gcgtcgccgg
     2281 aaatgaaggg acgaaggatc aaccggtccg tccgcaaggt gggctgcgtc attcaggaga
     2341 ggacttcgga gattcgatat tcgaccgctc ccgggagcag gcggccttcg cccacggcca
     2401 tcacgcgatt cagccaaagg taacggggat cgccggtttc aaatcgcggt tggatgatga
     2461 aatgggtgtc gccgaattcc gtcgcacccc cgccggccag ggcggcggcg a
//
LOCUS       DATDAY010000293         3213 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_660961, whole genome shotgun sequence.
ACCESSION   DATDAY010000293 DATDAY010000000
VERSION     DATDAY010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 3213)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3213)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3213
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_660961"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..244)
                     /locus_tag="VIS74_05900"
     CDS             complement(<1..244)
                     /locus_tag="VIS74_05900"
                     /inference="COORDINATES: protein motif:HMM:NF013921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEY5792812.1"
                     /translation="MPQSLSSLLIHLAFSTKNREPCLTGKAGAEIHPYLAGVLNNLDC
                     PSLQAGGASDPVHLFFRLSRTRAMAEIVEALKTSSSK"
     gene            complement(388..1653)
                     /locus_tag="VIS74_05905"
     CDS             complement(388..1653)
                     /locus_tag="VIS74_05905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY5792813.1"
                     /translation="MIQPIKRAKTEWILFWIDVDEPVHTGRGFILPTLIVVSDPDGVP
                     IGPPELMEELDQSRAENLLHKLFEQHTPPDRVTIGASEDWDPEAWKAFSREAGVEIRF
                     QKFNQNDPASLEQIFRTFSFGLQKGGPVENPREVARGLVATALQLRSPRKRLSLFKKA
                     LERDPDCAAARIELADLTFQDGHWKKALDAYEETLAREEPRWRNRYPVWWEDLETRPY
                     LRAHYGRAMTLWHLGHHAEAADGFETLLEMNPTDHQGARFLIPLVWLLADRQEVVTEF
                     FEYYEARYPKDFSEPAFLFGWAFSLHNLGKETEAKAKYREGILKNLYLAPLLLEAPEP
                     PANLWHPNDRAEPAYANEFIESYAVLWDRDPAALRLVRETYEELLPLVQQILAHRQQM
                     IDFQDQRYDPEYRAKWQALIAEDERLAGR"
     gene            complement(1650..2216)
                     /locus_tag="VIS74_05910"
     CDS             complement(1650..2216)
                     /locus_tag="VIS74_05910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HEY5792814.1"
                     /translation="MPENIVYFDLETQKTANDVGGWDQKHKMLMSIGVIYSTALGEYR
                     IFRENQVEELIQHLVKADLVVGYNTVNFDYQVLMGYTILDLPAMLHSLDLLVEIEKQI
                     GARVKLESVAQATLGVGKTAEGLEAILWWRQGRLMEIAEYCCFDVKVTKLIHEYGLEH
                     GEILYEDRFGRPQRLPASWAKKTKAALP"
     gene            2518..>3213
                     /locus_tag="VIS74_05915"
     CDS             2518..>3213
                     /locus_tag="VIS74_05915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY5792815.1"
                     /translation="MPNEFDRQVNKKAFQINMDPKIYGTFAEIGAGQEVARKFFQVGG
                     AAGTVAKTMSAYDMTFSDAIYGASGRYVCRQRLWTMLDHEFELLLERLDPTVGAERTF
                     FVFANTVAARSFRRHDESHGWLGVRFQTAPRSPVHEIIIHVRMLDAENVQQQEALGII
                     GVNLIYGAFFLAKRPEALIGSLMDDLSPGRMEVDMIRFSGPDFEKLDQRLMALRLVTQ
                     GLTKSALFRADGEV"
BASE COUNT          608 a          977 c          978 g          650 t
ORIGIN      
        1 atttcgagga gctggttttt agcgcttcga caatctcagc catggcgcgt gtccgcgaaa
       61 gccggaagaa gaggtgaaca gggtcagaag cgccgccggc ttggagggaa gggcaatcga
      121 ggttgttcag caccccggcc aggtaaggat gaatctccgc ccccgccttt ccggtcaaac
      181 aaggctcacg gtttttcgtg ctgaaggcga gatggatcag gagactggag agcgattggg
      241 gcatgtttgt ggaaaagaat cgcggccttg caggccgcag tgccgtggcg atcataaccc
      301 agcccgctgg gctgggttat gggatactgc gccttcggcg cagaccagaa tggccaaacc
      361 ccgcgcgggg gccggatgcc cccgccgcta tcttccggcc aggcgttcgt cctcggcgat
      421 gagcgcctgc catttggccc ggtattcggg atcgtaacgc tggtcttgaa aatcgatcat
      481 ttgctggcgg tgggcgagga tctgctgaac cagcggcagc aattcctcat acgtctcgcg
      541 caccagccgc agcgcggcgg ggtcgcggtc ccacagcacc gcgtagcttt cgatgaattc
      601 gttcgcgtag gccggttccg cgcggtcgtt cggatgccag agattggccg gcggctccgg
      661 cgcttcgagc aggagcgggg ccagatagag atttttgaga atgccttcgc ggtatttcgc
      721 cttggcctcc gtctccttgc cgaggttgtg aaggctaaac gcccagccaa agaggaacgc
      781 gggttcggag aaatccttcg ggtagcgcgc ttcgtaatat tcaaaaaatt ccgtgacgac
      841 ttcctgccgg tcggccagca gccagaccag cggaatcaaa aaacgcgcgc cctgatggtc
      901 cgtgggattc atttccagca aggtctcgaa gccgtccgcc gcctcggcgt ggtggcccag
      961 atgccagagc gtcatcgcgc ggccatagtg cgcccgcaaa tacggccgcg tttccagatc
     1021 ctcccaccag acgggataac gattccgcca gcgcggctcc tcgcgggcca gggtttcctc
     1081 gtaagcatcc agggcttttt tccagtgccc gtcctgaaac gtgagatcgg ccagttcgat
     1141 gcgggccgcg gcgcagtccg gatcccgctc cagcgccttt ttgaacaggc tgagccgttt
     1201 ccgcggactg cgcagctgta gcgccgtggc cacgaggccg cgggcgacct cgcgcggatt
     1261 ttcgaccggg ccgcccttct gcaggccgaa gctgaaggtg cgaaaaatct gctccagcga
     1321 ggcgggatca ttctggttga atttttgaaa gcgaatttcc acgccggcct cgcgcgaaaa
     1381 cgccttccac gcctcgggat cccaatcctc gctcgcgccg atggtcacgc ggtccggcgg
     1441 agtatgttgc tcgaagagct tgtggagcaa attttcggcg cggctttggt ccagctcctc
     1501 catgagctcg ggcggcccga tcggaacgcc gtccggatcg ctgacgacaa tgagcgtggg
     1561 cagaatgaat ccgcgtccgg tgtgaacggg ttcgtccaca tcgatccaaa acaaaatcca
     1621 ctccgtcttg gcgcgtttga tgggttgaat catgggaggg ccgccttcgt cttttttgcc
     1681 cagctcgccg gcaggcgctg cgggcggccg aagcggtctt cgtaaaggat ctcgccgtgc
     1741 tcgaggccgt attcgtgaat cagcttggtg accttcacgt cgaaacagca atattccgcg
     1801 atctccatca gccggccctg ccgccaccac aggatggctt ccaggccctc cgcggttttg
     1861 ccgacgccga gggtcgcctg cgccacggat tccagcttca cgcgcgcgcc gatttgtttc
     1921 tcgatttcga cgaggagatc gagcgagtga agcatcgcgg gcaggtcgag gatcgtgtag
     1981 cccattaaca cctgatagtc gaaattcacc gtgttgtaac cgaccaccag gtccgccttc
     2041 acgaggtgct ggattaattc ctcgacctga ttttcgcgaa agatgcggta ttcgcccagc
     2101 gcggtgctgt agatgacgcc gatggacatg agcatcttgt gtttctgatc ccagccgccc
     2161 acgtcattcg cggttttctg ggtttcgaga tcgaaataga cgatattttc cggcacggaa
     2221 aaacactacg gcaacacgct ccggcgccgc aaagctaaaa cgagggctcc cgccgcccgc
     2281 tctcgttgaa ccacggatgc acaccgatga acacggataa ggactgaaaa gaattttttg
     2341 ccgcaaggaa cgcaaaatat ccggaaggtg ggacggcagg ctgccgatgc tcttgaattt
     2401 tgaatccgtg actatcctgt tcatccgtgg ttcaatctcc ccgggctccg gcggggaaac
     2461 aaaattttct caattcttcc cctgtgctcc cggtggccgc tgtggttaac tcgggtcatg
     2521 cccaacgaat ttgaccgcca ggtgaacaaa aaggcgtttc agatcaacat ggacccgaaa
     2581 atctacggca ccttcgccga gatcggggcc gggcaggaag tcgcccggaa attttttcag
     2641 gtcggcggcg cggccgggac cgtggccaag accatgtcgg cttacgacat gacgttcagc
     2701 gacgccattt acggcgcgag cgggcgttac gtctgccggc agcggctgtg gaccatgctg
     2761 gatcacgaat ttgaactgct gctggagcgg ctcgacccga ccgtcggcgc ggaacgcacc
     2821 ttctttgttt ttgccaacac ggtggccgcc cgcagcttcc gccggcacga cgaatcccac
     2881 ggctggctcg gcgttcgctt ccagaccgcg ccgcgctcgc cggtgcatga aatcatcatc
     2941 cacgtccgca tgctcgacgc cgaaaacgtc cagcagcagg aagcgctcgg catcatcggc
     3001 gtcaacctca tctacggcgc gttcttcctc gccaagcggc ccgaggcgct cattggctcc
     3061 ctgatggacg acctctcccc gggccgcatg gaggtggaca tgatccgctt ctccgggccg
     3121 gatttcgaga agctcgacca gcgtttgatg gcccttcggc tcgtgacgca gggattgacg
     3181 aagtccgccc tctttcgggc cgacggcgag gtg
//
LOCUS       DATDAY010000294         2240 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_795454, whole genome shotgun sequence.
ACCESSION   DATDAY010000294 DATDAY010000000
VERSION     DATDAY010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2240)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2240)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2240
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_795454"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(3..1790)
                     /locus_tag="VIS74_05920"
     CDS             complement(3..1790)
                     /locus_tag="VIS74_05920"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY5792816.1"
                     /translation="MSVYARTVRYFIPFWWQTLVGALLILATTAFSLLKPWPFKYLID
                     GVLTVENTPAARQSREFLARWIPDSAPLQIVFWLCGALILINFLAGLLNLASNFLLLR
                     TGLVALMKLRTDLYAYLQSLPLKFHDSRRTTDSAFRVAYDSQSIQTLYNKGFATIFSS
                     VVMLIGTFVVMYFMNWQLTLASLVVLPAVVFAVRYFAVRIRNQSTTIQERESDLLTVV
                     QEGLSAIRMVHAFGREDFEVRQFRKRAVKSLEANLQLNLTSVVSALVVATLMAGGTAL
                     LYYIGSKQVLEGNLTLGDLTVFATYLVMLYQPLEQITYTVWAVEGATAGARRCFEVLD
                     RANDVPEMPHAKKLPAAKGEIRFENIQFSYDGSRRILQDINLTIPAGQKVAVVGGTGE
                     GKSTLLSLVPRFYDPDGGTIYLDGHDLRTLTKKSLREQISIVLQDTLLFSTTIRENIA
                     YGRALATEEEILEAARKAQALDFIQKLPDGLGSQVGERGSQLSVGQRQRIGIARAFLK
                     NAPILLLDEPTSALDPTTEHSIMDAIFELMEGRTTMIVTHRLATIHQIPRIVVLKNGR
                     VAEDGAGPELVAKGGIYAGLYRSGQYAAG"
     gene            2048..>2240
                     /locus_tag="VIS74_05925"
     CDS             2048..>2240
                     /locus_tag="VIS74_05925"
                     /inference="COORDINATES: protein motif:HMM:NF012788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792817.1"
                     /translation="MRILSGIQPSGTLHLGNYFGMMKPAIELQEQGEAFYFIADLHAL
                     TTVHDARALRQYVRDVAIDF"
BASE COUNT          433 a          707 c          676 g          424 t
ORIGIN      
        1 actcaacccg ccgcgtattg cccggaacga tacaggccgg cgtaaatgcc gcccttggcc
       61 accagctcgg ggccggcacc gtcttcggcc acgcggccgt ttttcaggac cacgatgcgc
      121 ggaatttgat gaatcgtcgc cagccggtgc gtgacgatca tggtcgtgcg gccttccatc
      181 aattcgaaga tcgcgtccat gatggaatgc tccgtcgtcg gatccagcgc cgacgtgggt
      241 tcgtcgagca gcaggatcgg tgcatttttc aggaacgccc gcgcgatgcc gatgcgctgc
      301 cgctggccca cgctgagctg gctgccgcgc tcgcccacct ggctgccgag gccatccggc
      361 agcttctgga tgaaatccag cgcctgcgcc ttccgcgcgg cctcgaggat ttcctcctcc
      421 gtcgccaagg cgcggccgta ggcgatgttt tcgcggatcg tcgtggagaa gagcagcgtg
      481 tcctgcagca cgattgaaat ctgctcgcgc agggactttt tcgtcagcgt ccgcagatcg
      541 tgcccgtcga gataaatcgt gccgccatcc ggatcgtaaa accgcggcac caggctcagc
      601 agcgtgctct tcccctcccc ggttccgccg acgaccgcca ccttctgccc ggcgggaatc
      661 gtcagattga tgtcctgcaa aatccgccgc gagccatcgt aggaaaattg gatgttctcg
      721 aagcgaatct cccccttcgc cgcgggcaat ttcttcgcgt gcggcatttc cggcacgtca
      781 ttggcgcggt ccagcacctc gaagcagcgc cgcgctcccg ccgtcgcgcc ctccaccgcc
      841 cagaccgtgt aggtgatctg ctcgagcggc tggtaaagca tcaccagata ggtcgcgaac
      901 accgtcagat cccccagcgt cagattgccc tccagcacct gcttggaacc gatgtaataa
      961 agcagcgccg ttccccccgc catgagcgtg gcgaccacca gcgcgctcac caccgaggtc
     1021 aggttcagct gcaaattcgc ctccaggctc ttgaccgcgc gcttgcggaa ctgccgcacc
     1081 tcgaagtcct cccggccaaa ggcgtgcacc atgcggatcg cgctcaaacc ctcctgcacg
     1141 accgtcagca gatcgctttc ccgctcctga atggtcgtgg attgattgcg aatccgcacc
     1201 gcgaaatacc gcaccgcaaa gaccaccgcc ggcaggacga ctagcgacgc gagggtgagc
     1261 tgccaattca tgaaatacat caccacgaac gtgccgatga gcatgacgac ggagctgaaa
     1321 atggtcgcga accccttgtt gtaaagcgtc tgaatggatt gggaatcgta ggccacgcgg
     1381 aaggcggaat ccgtcgtgcg gcgcgagtcg tggaacttga gcggcaggga ttgcagatac
     1441 gcgtagaggt ccgtccgcag tttcatcaat gccaccagcc ccgtgcgcag cagcaggaaa
     1501 ttcgaggcga ggttgagcag tcccgcgagg aagttgatga ggatgagcgc cccgcagagc
     1561 cagaacacga tctgaagcgg cgccgagtcc ggaatccagc gcgcgaggaa ttcccgggat
     1621 tgccgggccg ccggggtgtt ttcgacggtc agaacgccat cgatgagata cttaaacggc
     1681 cacggcttga gcaggctgaa agcggtggtg gcgaggatca acagggcgcc aaccagcgtc
     1741 tgccaccaga aggggataaa atagcgcacc gtgcgcgcgt aaacactcat gggaagtggc
     1801 gattagtaac gaatcgcccg aggcgctgca atcaataccg caggttttgg ccgaaccacg
     1861 gatggatacg gatgaacgcc gatttttttg cggctttgcg gactttgcgt gggctggctt
     1921 tcgctgattc gacggcggcg acggcgcgcc gccctccagg ttggatgaaa tatccgtggt
     1981 tcccctcggc agctcccccc gccttgactt cccttacccg gctttgggag gatgcgcgtt
     2041 tgattttatg aggattcttt ccggcattca gccttcgggg acgctacacc tgggcaatta
     2101 ttttggcatg atgaagcccg ccatcgagct tcaggagcag ggggaggcgt tttatttcat
     2161 cgccgatctc catgcgctga cgacggtgca cgacgcccgg gcgctgcgcc aatacgtgcg
     2221 ggacgtggcc attgacttcc
//
LOCUS       DATDAY010000295         2558 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_2219359, whole genome shotgun sequence.
ACCESSION   DATDAY010000295 DATDAY010000000
VERSION     DATDAY010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2558)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2558
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2219359"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..819
                     /locus_tag="VIS74_05930"
     CDS             <1..819
                     /locus_tag="VIS74_05930"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEY5792818.1"
                     /translation="ELMKILAVSNQKGGVGKTTTSLNLSAALAERGLKILLIDLDPQG
                     NASGVLAGKENEHPGIYQVLTESLPPAACIQPTRLANLWVMTANLDLAGAEVEVARLE
                     EHQFQLRKSLEPLRAESAYDYVIFDCPPSLGILMSNALVAADEILMPIQCEYYALEGL
                     GLLMQVTDHIRSSGTNPTLAISGMVMTMYDSRTNLNAAVVRDVRAHFEDVIYETLIPR
                     TVRFGEAPSHGLTILEYDPSGTGTAAYRALAEEFLQRQAKGISFAGYNQAPAES"
     gene            1020..1598
                     /gene="pyrE"
                     /locus_tag="VIS74_05935"
     CDS             1020..1598
                     /gene="pyrE"
                     /locus_tag="VIS74_05935"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEY5792819.1"
                     /translation="MNSDVFDLFRQSGALLEGHFILRSGLHSRQFFQCALLLQHTGIA
                     SDICGRLADKLRGYDCETVVSPALGGIIVGQEVARHLGKRHIFLEKDDQGALVLRRGF
                     TLRPGEKILVAEDVVTRGGRVQETIDIVRSLGGEVAAAGCIVDRSGGNHPDFGCPFVS
                     LVEMNVETFEADQLPPDLAGTPAVKPGSKAKA"
     gene            1656..1883
                     /locus_tag="VIS74_05940"
     CDS             1656..1883
                     /locus_tag="VIS74_05940"
                     /inference="COORDINATES: protein motif:HMM:NF021474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HEY5792820.1"
                     /translation="MKTTIDIPEKELRDAMRFTKAKTKKEAILAVLADYNRRQRMAEL
                     IKYSGTFDSLMTNEEIEALDEERTRRIWKTQ"
     gene            1880..2248
                     /locus_tag="VIS74_05945"
     CDS             1880..2248
                     /locus_tag="VIS74_05945"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HEY5792821.1"
                     /translation="MKLIDTSCWVHQLRRSGKPEIRQRVELLLASGQAAWCSIVRLEL
                     WNGVGNDQERKVLWEYEALIPEYPITDEVWEQACELASRCRRAGKTASGGDVLIAACA
                     RHHGVEVERADSHFDFLMTL"
     gene            complement(2276..>2558)
                     /locus_tag="VIS74_05950"
     CDS             complement(2276..>2558)
                     /locus_tag="VIS74_05950"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY5792822.1"
                     /translation="GRSASPEVAAYCASKWAIEGLTQALAQELPAGMAAVSLNPGIID
                     TDMLRECFGAAAGNYPTAAEWILRAGRFLRKLGPESNGRALTVPGIPVD"
BASE COUNT          530 a          838 c          725 g          465 t
ORIGIN      
        1 gaactgatga aaattctcgc cgtctcgaac caaaagggag gcgtgggcaa aaccacgacc
       61 tcgctcaatc tctcggccgc gctcgccgag cgggggctga aaatcctgct catcgatctc
      121 gatccgcagg gcaacgccag cggcgtgctg gccggcaagg agaatgaaca tcccggcatc
      181 taccaagtgc tcaccgaatc cctccctccc gccgcctgca tccagcccac gcggctggcg
      241 aatctctggg tcatgaccgc caatctcgac ctcgccggcg ccgaagtgga ggtcgcccgg
      301 ctggaggagc accaattcca gctgcggaaa tccctggagc cgctccgggc cgaaagcgcc
      361 tacgattacg tcatcttcga ctgcccgcct tcgctgggca tcctgatgtc gaacgcgctc
      421 gtcgccgcgg atgaaatcct catgccgatc caatgcgaat attacgcgct cgaaggcctt
      481 ggactgctca tgcaggtgac cgaccacatt cgcagctccg gcaccaatcc cacgctcgcc
      541 atctcgggca tggtgatgac catgtatgat tcccgcacca atctcaacgc cgccgtcgtc
      601 cgggatgtcc gcgcgcactt cgaggacgtg atttacgaaa cgctgatccc gcgcaccgtc
      661 cgcttcggcg aggcccccag ccacgggctc acgatcctgg aatacgaccc ctccggaacc
      721 ggaaccgcgg cttaccgcgc cctggccgag gaatttctcc agcgccaggc caagggcatc
      781 tcgttcgccg gttacaacca ggctcccgcc gaaagctaat cctgattttt taaccacaga
      841 gaacacagag aacacagaga acacagagaa cacagagaac acagagaaca cagagaacac
      901 agagaacaca gagaacacag agaagggaaa acggggttcg atctcggggg tattgccttt
      961 gcatcccttt cgttttttct ctgtgttctc ggtgctcact gtggttaatt tccccctcca
     1021 tgaattccga cgtttttgat ttgttccgcc agtccggcgc gctgctggaa ggccatttca
     1081 tcctgcgctc cggcctgcac agccggcaat ttttccaatg cgccctgcta ctgcagcata
     1141 cgggcatcgc ttccgatatc tgcggccggc tcgccgataa attgcgcggt tacgattgcg
     1201 agaccgtcgt gtcccccgcc ctcggcggca tcattgtcgg ccaggaagtc gcccggcacc
     1261 tcggcaagcg gcacattttc ctcgaaaaag acgatcaagg cgcgcttgtg ttgcggcgcg
     1321 gtttcaccct tcgccccggt gaaaaaatcc tcgtcgccga ggacgtcgtc acgcgcggcg
     1381 ggcgggtgca ggagaccatc gacatcgtcc gctcgctcgg cggcgaggtc gcggccgcgg
     1441 gctgcatcgt ggaccgcagc ggcggcaatc atcccgattt cggctgcccg ttcgtctccc
     1501 tggtcgagat gaacgtcgaa accttcgaag ccgatcaatt gcctcccgac ctcgccggca
     1561 ctccggcggt gaaacccggc agcaaagcca aagcctgagc gggcgggaat ccgttttcat
     1621 tgaatccgcc ttcccaatct gctttattgg gaagcatgaa aacgaccatt gatattccgg
     1681 aaaaagaact ccgggatgcc atgcggttca ccaaggcgaa aaccaaaaaa gaggcgatcc
     1741 tggccgtgct cgcggactac aatcgccgcc agcgcatggc ggagttgatc aagtattcag
     1801 gcacattcga ttcactgatg accaacgagg aaatcgaggc cctggatgaa gagcgcaccc
     1861 ggcgaatctg gaagacccaa tgaaattgat tgatacctcg tgctgggtgc atcaactgcg
     1921 gcgatccgga aaacccgaga ttcgacaacg cgtggagctg cttttagcca gcggccaggc
     1981 ggcttggtgc tcgattgtcc gcctggaact ctggaacggc gtgggaaacg accaggaacg
     2041 aaaagtgctg tgggagtatg aagccctcat ccccgaatat cccatcacgg acgaggtctg
     2101 ggaacaggcc tgcgaactgg ccagccgctg ccgccgggcg gggaaaaccg cgtcgggggg
     2161 cgatgtcctg atcgccgcct gcgcccgcca tcacggagtc gaagtcgaac gcgcggactc
     2221 gcatttcgat tttttgatga cgctttgagg caagcggccg gctggcatcc gtcgctcaat
     2281 ccaccggaat ccccggcacc gtcaacgccc ggccgttgct ttccggcccc agtttgcgca
     2341 ggaaacggcc cgcgcggagg atccactccg ccgccgtggg ataatttccc gccgccgcgc
     2401 cgaaacattc gcgcagcatg tcggtatcga tgatccccgg attcagcgac accgcggcca
     2461 ttcccgccgg cagttcctgc gcgagcgcct gggtcagccc ttcgatggcc cacttcgacg
     2521 cgcaataagc cgccacttcc ggcgaggcgc tccggccc
//
LOCUS       DATDAY010000296         5802 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_2519044, whole genome shotgun sequence.
ACCESSION   DATDAY010000296 DATDAY010000000
VERSION     DATDAY010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 5802)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5802)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5802
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2519044"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..269
                     /locus_tag="VIS74_05955"
     CDS             <1..269
                     /locus_tag="VIS74_05955"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF013845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA dimethylallyltransferase"
                     /protein_id="HEY5792823.1"
                     /translation="EGVVEEVRRIGETGGTAAKAIGLAEIRQLIEGSLTPAECRERIV
                     NATRQYAKRQLTWFRAQASYTSIPLTQLEPITHSLGQALRLTHD"
     gene            262..1551
                     /gene="hflX"
                     /locus_tag="VIS74_05960"
     CDS             262..1551
                     /gene="hflX"
                     /locus_tag="VIS74_05960"
                     /inference="COORDINATES: protein motif:HMM:TIGR03156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEY5792824.1"
                     /translation="MTDVKPRSAQEKTILVGLEHAGVSHWDVADSLDELRQLAATAGA
                     QVVDTFVQKLDKPTAPYYIGKGKAEEIARACGSAQATSLIFDDELSPAQGRNLEELTS
                     RKVIDRTQLILDIFAQRARSKEGRLQIELAQLQYLLPRLTGMWTHLSRQSGGIGTRGP
                     GETQLEVDRRRVQERIARIERDLKDVRRNRNVQREGRLRRNWPAASLVGYTNAGKSSL
                     LNRLTGAGVLAEDKLFATLDPTTRQVTLPNRQRILLTDTVGFIRKLPHTLIESFKATL
                     EEVALADLLIHVIDLSHPQFHEQMAAVDVTIRELDADGKQTVLVFNKTDRIANPEIIF
                     AQLARYPGSVAISAQTGEGIDSLIQELEMRLQAWRLRVQFRIPHSEPALLAEMHRVGH
                     VLSASYEEEVVLVAAHIPPEHKNRFSAFEITAVPAGL"
     gene            1548..2000
                     /locus_tag="VIS74_05965"
     CDS             1548..2000
                     /locus_tag="VIS74_05965"
                     /inference="COORDINATES: protein motif:HMM:TIGR00413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HEY5792825.1"
                     /translation="MRGLLRLAAGLGLAPFLLSSCAGWPPPSSPRIYSHQEVVRTQEG
                     TASYYGGRWIGRLTASGERYRANDLTAAHRKLPFDSVVRVTNLKNQKSVIVRINNRGP
                     YARGRMIDLSIAAAKRIDMVDAGVARVKLEVLKEIPVLMKPNLRKREE"
     gene            complement(2022..3056)
                     /locus_tag="VIS74_05970"
     CDS             complement(2022..3056)
                     /locus_tag="VIS74_05970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792826.1"
                     /translation="MSATAAAFPAPPTRKARIKKMILTVILISVGIHVLGGIVAGFWI
                     VAKYFSNPPAEFKVVKDIRIPARQREHSMNMAALDSLAPKPSFNDKMQSLRPTAFALP
                     ELPKVPMDQMLPLDPSAIVSDAVSSLVGAGGLGGGGGGGLGGMGGTGTGFSFFGIQSN
                     AKRILLLFDVSGSVVNKANKAGIPLSKIKEETLNTINGLTPNSRFGMIQFTQNYKPFQ
                     AELVPATDRNKQAAKTWVEQEWVESGSMGAGGKVVSNARGFLGVLEWAAQARPDVIFV
                     ISDASFQWKPGGQIANIPWKELQDFATGSLQGAEGCPIHFIGFEMKPGDRREAGILAR
                     RTQGKVRELK"
     gene            complement(3056..3496)
                     /locus_tag="VIS74_05975"
     CDS             complement(3056..3496)
                     /locus_tag="VIS74_05975"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEY5792827.1"
                     /translation="MKRIEHAQIELQIAPMIDVCFLLLFFYILTSKPVKPEADMSMTL
                     PGTVAQEESVDIPDEQRIQIQDSGQIILNEMPIGAAGDSRLEKLVTTLKRFKESTDAN
                     KSTAMVTLDAMDTAPHQRIIDVLNACAAAGIQGVTFADNAAGEE"
     gene            complement(3493..3930)
                     /locus_tag="VIS74_05980"
     CDS             complement(3493..3930)
                     /locus_tag="VIS74_05980"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEY5792828.1"
                     /translation="MATQVRRARRRVVHEEVGLQIAPMIDVVLLLLFFFMLSGKLTKD
                     AKLMDIQMPTAVSAQVPKDMGDRDVVNIDEKGQLFSGDQAVTAKELTAHLKERFKNFP
                     PLKLYVRADSRTPAKKIKEVMKIASEAGAIEVVFGVLQSGSQS"
     gene            complement(3939..4745)
                     /locus_tag="VIS74_05985"
     CDS             complement(3939..4745)
                     /locus_tag="VIS74_05985"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HEY5792829.1"
                     /translation="MKKKPKTKLPNLPAAGLLAGALLGLAMSDASAMGGVESGKSLLD
                     LYREGGTVMHLIALCSIATLSLGAYCGILIRRQRVMPAPVVAQLSQLMAQRDVAGAYE
                     ACKANPGPFTNALSSALLKANFQRDMYNKPAMENAITDECFREETRLMALVNYLNTFA
                     VLAPMIGLLGTVSGMITSFSALTAGKAEATDLAKGIGEALICTGGGLLLAIPAMFLYF
                     YFRGQASVLMGDVHEKLSGMLDLFTGEVTPQSIYAVTGEAAEPGVIAEQA"
     gene            complement(4714..5664)
                     /locus_tag="VIS74_05990"
     CDS             complement(4714..5664)
                     /locus_tag="VIS74_05990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792830.1"
                     /translation="MKTRLLSLLLLLTAALLPAQDVLFMNNGEKRAGKLIGVDAKIYR
                     LQIPLPVPAGAQSAGFATATVPRTEVARIEFADDPARDEKLATATPARLAEVEPLWKQ
                     AEPWLGIPKSPAGKIAAAYGALLLKSGQPENAARALALFQRIETESWSEADHALARQG
                     RLQALIATGRAAEAAAEAAKLARETEDPAIVLQAKFILAEAADQSLRTLVAENPRWEE
                     DVHVIPERARLMNAALDDYLYASLFFGSEIEPAARGLWGATSVYQFTGQTELAVETCR
                     DLLALYPATSSARLAQDFLNTLTDEQKQTDYEKEAKDETP"
BASE COUNT          954 a         1786 c         1971 g         1091 t
ORIGIN      
        1 cggagggggt ggtcgaggaa gtgcggcgga tcggggaaac gggcggcacc gcggcgaagg
       61 cgatcggcct ggccgagatc cggcaattga tcgagggttc gctgactccg gccgaatgcc
      121 gggagcggat cgtcaatgcc acccgccaat acgccaaaag gcaattgacc tggttccgcg
      181 cgcaggctag ttatacttcc atccctttga cgcagttgga acccatcact cattccctcg
      241 ggcaggcgct tcggctgact catgactgac gttaagcccc gctctgctca agaaaagacg
      301 atcctggtcg gactggagca tgccggcgtt tcgcattggg atgtcgcgga ttccctggat
      361 gagctccgcc agctggcggc gacggccggc gcgcaggtcg tggacacctt cgtgcagaaa
      421 ctcgacaagc ccaccgcgcc ttattacatc ggcaagggca aggcggagga gatcgcccgg
      481 gcctgcggga gcgcgcaggc gacgtcgctg atttttgacg acgagctttc ccccgcccag
      541 gggcggaatc tggaggagct gacttcgcgc aaggtcatcg accggacgca gctcatcctc
      601 gacatcttcg cccagcgggc gcgctcgaag gaggggcgtt tgcagatcga gctcgcgcaa
      661 ttgcaatacc tcctcccgcg cctgaccggc atgtggaccc acctttcgcg ccagtccggc
      721 ggcatcggca cccgcggtcc gggggaaacg cagctggagg tggaccgccg gcgggtgcag
      781 gagcggattg cccgcatcga gcgcgacctc aaggacgtgc gccggaaccg gaacgtgcag
      841 cgggaggggc gcctgcgccg gaactggccg gcggcttcgc tggtgggtta taccaatgcc
      901 ggcaagtcct ccctgctcaa ccgcctcacc ggtgcggggg tgctggcgga ggataaattg
      961 ttcgccacgc tcgatcccac cacgcgccag gtcacgctgc cgaaccggca gaggattctc
     1021 ctgacggata cggtggggtt catccgcaag ctgccccaca cgctcatcga atccttcaag
     1081 gcgacgctgg aggaggtggc gctggcggac ttgctcattc acgtcatcga tctgagccat
     1141 ccgcagttcc acgaacaaat ggccgcggtg gatgtgacga tccgcgaact ggatgccgac
     1201 gggaagcaga ccgtgctggt cttcaacaag acggaccgga tcgccaatcc cgaaatcatc
     1261 ttcgcgcaat tggcgcgtta tcccggcagc gtggccattt ccgcccagac gggcgagggc
     1321 attgattcgc tcattcagga gctcgaaatg cggctgcagg cctggcgcct gcgggtgcaa
     1381 ttccgcattc cgcattccga accggcgctg cttgccgaga tgcaccgcgt cggccacgtg
     1441 ctgtcggcca gctatgagga ggaggtggtg ctcgtggcgg cccatattcc tcccgagcat
     1501 aaaaatcgct ttagcgcgtt tgagatcacc gcggttccgg ctgggttatg aggggtcttc
     1561 ttcggctggc cgccggcctt gggctggcgc cgttcctgct ttcttcgtgc gccgggtggc
     1621 cgccgccgtc ctcgccccgc atttattccc atcaggaagt ggtgcggacc caggaaggca
     1681 cggcgtccta ctacggcggc cgctggatcg gaaggctgac cgccagcggc gagcggtacc
     1741 gcgccaatga tctgacggcg gcgcatcgca agctgccatt tgactcggtg gtccgcgtca
     1801 cgaacctgaa gaatcaaaaa tccgtgatcg tccggatcaa taaccgcggc ccctacgcga
     1861 gggggcggat gattgatctg agcatcgccg ccgcgaagcg gatcgacatg gttgatgcgg
     1921 gggtcgcccg cgtgaagctg gaggtcctga aggaaatccc agtgctgatg aagccgaatc
     1981 tgcgcaagcg ggaggagtaa ttttctgaac cgccccggcg gttatttcag ctcccgcacc
     2041 ttgccctggg tccgccgggc caggatgccg gcctcgcggc ggtcgccggg cttcatctca
     2101 aaaccgatga agtgaatcgg gcagccctcc gcgccctgca gcgagccggt ggcgaagtcc
     2161 tggagttcct tccacggaat gttcgcgatc tgcccgccgg gcttccactg aaagctggcg
     2221 tccgagatga cgaagatcac atccgggcgc gcctgggcgg cccattcgag cacgcccagg
     2281 aaaccgcggg cattggagac gactttgccg cccgcgccca tggagccgga ctccacccat
     2341 tcctgctcga cccacgtctt ggcggcctgc ttgtttcggt ccgtggcggg caccagctcc
     2401 gcctggaagg gcttgtagtt ctgggtgaac tggatcatgc cgaaacgcga attcggcgtg
     2461 aggccattga tcgtattcag cgtctcctcc ttgattttgc tgaggggaat ccccgccttg
     2521 ttggccttgt tcaccacgct gccggataca tcaaaaagca gcaggatgcg cttggcgttg
     2581 gattgaatgc cgaaaaagct gaatcccgtg ccggtgccgc ccattccccc cagtccgccg
     2641 ccgcccccgc cgccgaggcc gccggcgccg accaggctgg aaaccgcgtc cgagacgatc
     2701 gcgctggggt ccagcggcag catttgatcc atcggcacct tcggcagctc cggcagcgcg
     2761 aaggccgtgg ggcgcaggga ctgcatcttg tcgttgaagc tgggcttggg cgccaggctg
     2821 tccagcgcgg ccatgttcat ggagtgttcg cgctgccgcg cggggatgcg gatgtccttc
     2881 accaccttga actccgcggg cggattggaa aaatattttg cgacgatcca gaatcccgcc
     2941 acgatgccgc cgagcacgtg aatgccgacg ctgatgagga tcacggtgag gatcattttt
     3001 ttgatccgcg ccttgcgggt ggggggagcg gggaatgcgg cggcggtggc gctcatcatt
     3061 cttctcccgc ggcattgtcg gcaaaggtca cgccctggat gccggcggcc gcgcaggcgt
     3121 tcagcacgtc gatgatgcgc tgatgcggcg cggtgtccat ggcgtccagc gtgaccatgg
     3181 ccgtggattt gttggcgtcg gtggattcct taaaccgctt gagcgtcgtc accaattttt
     3241 ccagccggct gtccccggcg gcgccgatgg gcatttcgtt gagaatgatc tggccggaat
     3301 cctggatctg gatgcgctgc tcgtcgggga tgtccacgct ttcctcctgc gccacggtgc
     3361 cgggcagggt catgctcatg tccgcttccg gtttgaccgg cttcgaggtc aggatgtaga
     3421 aaaagagcag caggaaacac acgtcgatca tcggcgcgat ctggagttcg atctgggcgt
     3481 gctcgatgcg tttcatgact gggatccgga ttgcaagacg ccgaagacca cttcgatggc
     3541 gcccgcctcc gaggcgattt tcatgacttc cttgatcttc ttcgccggcg tgcgcgagtc
     3601 ggcgcggacg tagagcttga gcggcgggaa attcttgaac cgctccttca ggtgcgcggt
     3661 gagttccttg gcggtcaccg cctgatcgcc ggagaagagc tggccctttt catcgatgtt
     3721 caccacgtcg cggtcgccca tgtccttggg aacctgcgcg gagaccgccg tgggcatctg
     3781 gatgtccatg agcttcgcat ccttcgtcag cttgccggag agcatgaaga aaaagagcag
     3841 cagcagaacg acgtcgatca tcggcgcgat ctggaggccc acttcctcgt ggacgacccg
     3901 gcgtctggct cggcggacct gggtggccat cgttccgatc aggcttgttc ggcgatgacc
     3961 ccgggctccg cggcctcgcc cgtgacggcg tagatgcttt gcggcgtcac ttcgccggtg
     4021 aagaggtcga gcattccgga gagtttttca tgcacgtcgc ccatgagcac cgaagcctgg
     4081 ccgcggaagt aaaagtagag gaacatggcc ggaatggcga gcagcaggcc gccgccggtg
     4141 cagatgagcg cctccccgat gcccttggcg aggtcggtcg cctcggcctt gccggcggtc
     4201 agcgcgctga agctcgtgat catgcccgac acggttccca gcagcccgat catcggcgcg
     4261 agcacggcga aggtgttcag gtaattgacc agcgccatga ggcgggtttc ctcgcggaaa
     4321 cactcgtcgg tgatcgcgtt ttccatggcc ggcttgttat acatgtcgcg ctggaaattg
     4381 gccttgagca gcgcggagga gagggcgttg gtgaacgggc cgggattcgc cttgcaggcc
     4441 tcataggcgc cggcgacatc ccgctgggcc atgagctggc tgagctgggc gaccaccggc
     4501 gcgggcatga cgcgctggcg gcggatgagg atgccgcaat acgcgccaag cgacagggtg
     4561 gcgatggagc acagcgcgat gaggtgcatg accgtgccgc cctctcggta gaggtccagc
     4621 aggcttttgc cgctttccac gccgcccatg gccgaggcgt cgctcatggc cagccccagg
     4681 agcgccccgg ccagcagtcc ggcggccggc aggttaggga gtttcgtctt tggcttcttt
     4741 ttcatagtcg gtttgttttt gttcgtcggt gagggtgttc aggaaatcct gggcgagccg
     4801 ggcggaggac gtggcggggt agagcgcgag gaggtcgcgg caggtttcca ccgccagctc
     4861 ggtctggccg gtgaattgat agacgctcgt ggcaccccag agcccgcggg cggcgggttc
     4921 gatttccgag ccgaagaaga ggctcgcgta aaggtagtca tccagcgcgg cattcatgag
     4981 gcgggcgcgc tcgggaataa cgtggacgtc ctcctcccag cgcggatttt ccgccacgag
     5041 cgtccgcagg ctctggtcgg ccgcctcggc gagaatgaat ttcgcctgaa ggacaatggc
     5101 gggatcctcc gtttcccgcg cgagcttggc cgcctccgcc gcggcttcgg cagcccggcc
     5161 ggtggcgatc aacgcctgca ggcgcccctg ccgcgcgagc gcgtggtcgg cctcgctcca
     5221 gctttccgtt tcgatgcgct ggaagagcgc gagcgcccgg gcggcattct ccggctggcc
     5281 gcttttgagc agcagtgccc cgtaggcggc ggcgattttc ccggcgggcg atttgggaat
     5341 ccccagccac ggctccgctt gtttccagag cggctcgact tccgccagcc gggccggcgt
     5401 ggcggtggcg agtttttcgt cgcgcgcggg atcgtcggcg aactcgatcc gcgccacctc
     5461 ggttcgcgga accgtggccg tggcaaatcc ggcggattgg gcgcctgccg ggacgggcag
     5521 cggaatttgc aggcggtaaa ttttggcatc cacgccgatg agtttgccgg cgcgcttttc
     5581 gccattgttc atgaacagca cgtcttgcgc gggcagaagc gcggcggtca gcagcagcag
     5641 cagggaaagc aggcgggttt tcatcccttg gcgtgctccg gggcggggga ttcgggcagc
     5701 gggccgccga gcgcgttgag ccgctcgcgg gcccggaccg tttccggtga gtgggtgaga
     5761 tcctccagct cggtgagttc ctgataggtg cggcgggcgg cg
//
LOCUS       DATDAY010000297         4132 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_455897, whole genome shotgun sequence.
ACCESSION   DATDAY010000297 DATDAY010000000
VERSION     DATDAY010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 4132)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4132)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4132
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_455897"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            137..631
                     /locus_tag="VIS74_05995"
     CDS             137..631
                     /locus_tag="VIS74_05995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792831.1"
                     /translation="MQSDWEIKGRAHTCSRTHRTFEEGEYFYTLLYRDGEGFRREDLS
                     ETAWAERNDNIQPFSFWRSKYEPPAPPPPEALKKDDAEGLLRQMLAEPKPGQTNACYI
                     LALMLERKRLIRPVESPDEGLLVYEHLKTGDVFTLRNPHLSIEQIPGVQREVAELLGA
                     PAKA"
     gene            663..1643
                     /gene="dusB"
                     /locus_tag="VIS74_06000"
     CDS             663..1643
                     /gene="dusB"
                     /locus_tag="VIS74_06000"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HEY5792832.1"
                     /translation="MNAFPLFLAPMAGVTNTVFRSICRERGADVLTSEFVSAEGIFHR
                     NQRTREYLEFSPAERPVGVQLFGGNPEHLAEAAKHVVDWVNPDFIDLNFGCPVNKVVC
                     KQGGSALLKDCPLLERVAAAVVRAVAPRPVTAKIRIGWDEQTINATRTARILEDCGIR
                     RLAVHGRTKAQGYSGEADWNVIAEVAAAAGIPVIGNGDITSAGVAVRRQRESGVAGLM
                     IGRAAMSNPWIFQEIRTLWETGVEAIPATLEQRWALILDHCRREIIWRKDENFAMRAM
                     RSRLMAYTRGMPGGRHLRGYLAQAASLGQLEGLAEEHLAEVESGIHLQPA"
     gene            1801..2178
                     /locus_tag="VIS74_06005"
     CDS             1801..2178
                     /locus_tag="VIS74_06005"
                     /inference="COORDINATES: protein motif:HMM:NF015898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HEY5792833.1"
                     /translation="MKPLPRISESEWSIMEVVWSEAPVAAAEIIEALGKSMNWKHQTI
                     RTLLARLVKKKALAFEPDGNRYLYRPLVKRADLVQAESDSFLRRVFAGAAQPLLVHFA
                     RKAKLSDEEIRELQEILARGSGK"
     gene            2175..>4132
                     /locus_tag="VIS74_06010"
     CDS             2175..>4132
                     /locus_tag="VIS74_06010"
                     /inference="COORDINATES: protein motif:HMM:NF017390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HEY5792834.1"
                     /translation="MSSAPEGIAEAFAWVLERSWQATGLVIFLLVLRWVFARGLSPWW
                     RHALWLVPVCLLILPELPECPLSVHRWKLETVKTGPLIALPVRSEPPPAILPSGGEMT
                     GFQEENPGAPEPSAGWMHWAGLGWASVAAGLLAFFGVVNLHFHIRLRSNGRPVDARAL
                     RIFEACRRELGVGRSGGVVETDLIDSPATAGIFRPRVLVPSGFGEALSEMEIRWIFLH
                     ELTHLKRGDLLMNAVVAVLGALHWFNPILAWAFRQMRADREAACDEAVLVHIGGEHRQ
                     AYGKSLLLWMRGGLGRFRDAGLVGMSASRREVQSRIEQIARFGGRRRGWSIVSAVLFI
                     GIAVTFLTRTAGSNAALGKEIPAANFARICDRNGVVLVEIEFTADGKTKARYPLGALA
                     ASLLAELDDAAKPKAGESICLTIDVRAQSVIENELRRVGRGAAVLLDPWNGDILAMAS
                     VPSFDPNGAFPVPSKTDLLDPRMNRALTAFTPGAVFLPVTALAAAAAGLDKETFECRG
                     SLPVGKYTMKCWKTAGHGPLGLPEALKASCNVYFYHCGIVLGERVTEMAGHLGFGLTS
                     GIPVRGEASGYIGNPEQPPKRFPSQVWSDGWAANRAIGQGDSQVTLLQVAVMMGTIGN
                     GGTVYWPRLVKRVIQNGESREQPAARIRA"
BASE COUNT          731 a         1187 c         1402 g          812 t
ORIGIN      
        1 gggcgacaaa cttccaccga tcattgccgc gtgggggccg atcgccgggt atttcgccgt
       61 cggcctggcg ttgctgtggt tccgctccac caatcgcgac ttgccgaagc tcaaaatccc
      121 gtggctgaat taacccatgc agagtgactg ggaaatcaag gggcgggcgc acacctgctc
      181 gcgcacgcac cgaaccttcg aagaggggga gtatttttac acgctgctct atcgcgacgg
      241 cgagggtttc cggcgcgagg atttgagcga aaccgcctgg gcggagcgca acgacaacat
      301 ccagcccttt tccttctggc gctcgaaata cgagccgccc gcgccgccgc cgcccgaggc
      361 gttgaaaaag gacgacgccg aggggctgct gcggcaaatg ctcgccgaac ccaagcccgg
      421 gcagaccaac gcctgctaca ttctcgcgct catgctggag cgcaaacgcc ttatccggcc
      481 ggtggaaagc ccggacgagg gcctgctcgt ttacgagcac ctcaagacgg gcgatgtctt
      541 cacgctccgg aacccgcatc tgtccatcga acagatcccc ggcgtgcagc gcgaggtggc
      601 cgaattgctg ggcgcccccg ccaaggcttg attgaggcgg cttcccgggc gagaatgccc
      661 ggatgaacgc ctttccgctc ttcctggcgc ccatggccgg ggtgaccaac accgtcttcc
      721 ggtccatttg ccgggaacgc ggggcggacg tgctgacgtc ggaattcgtt tccgccgagg
      781 gcatcttcca ccgcaaccag cggacgcgcg aatacctcga attttccccc gccgagcggc
      841 ccgtcggcgt gcagcttttc gggggaaatc ccgagcatct ggccgaggcg gcgaaacacg
      901 tggtggattg ggtgaatccc gattttatcg acctcaattt cggctgcccg gtcaacaagg
      961 tcgtctgcaa gcagggcggc tcggcgctgt tgaaggactg cccgctgctt gagcgcgtgg
     1021 ccgccgcggt cgtgcgcgcc gtcgccccgc ggccggtcac cgcgaagatt cgcatcggct
     1081 gggatgagca aacgatcaac gccacccgca ccgcccggat tctggaggat tgcggcatcc
     1141 ggcgcctcgc ggtgcacggc cgcacgaagg cgcagggtta ttccggggag gccgattgga
     1201 acgtgatcgc cgaagtcgcg gcggcggcgg gaatcccggt catcggaaat ggcgacatca
     1261 cctccgccgg ggtggccgtc cgccgccaga gggaatccgg cgtggccggc cttatgatcg
     1321 gccgggcggc gatgagcaat ccgtggattt tccaggaaat ccgcacgctt tgggaaaccg
     1381 gcgtggaagc gattcccgcg acgctggaac agcgctgggc cttgattctg gatcattgcc
     1441 gccgggaaat catttggcgg aaggatgaaa atttcgccat gcgcgccatg cgttcgcgcc
     1501 tgatggccta cacccgcggc atgcccggcg gccggcacct gcgcggctac ctggcgcagg
     1561 ccgcgagcct gggccagctc gaagggttgg cggaagagca tttggccgaa gtggagtccg
     1621 gtatccattt gcagcccgcg taatcttata gccacggacg gctcggcgag ccgtccctgc
     1681 ctaaaatggg cagggcgggt tcgatgaacc cgccgatttt ttcggtttgt cctcggaact
     1741 ctaaaaaatg attacatttg tggttgaata atgattacgc ctgtagtaat tcggtgactc
     1801 atgaaacccc tgccccggat ttcggaatcg gaatggtcga tcatggaagt cgtttggagc
     1861 gaagcgccgg tcgcggcggc ggagatcatc gaagccttgg gcaaatcgat gaattggaag
     1921 caccagacga ttcggacgct gctggcccgc ctggtgaaga aaaaggcgct ggccttcgag
     1981 ccggacggca accgctatct ttaccggcca ttggtcaagc gggcggatct ggtccaggcg
     2041 gagagtgact cattcctccg ccgggtcttt gcgggagccg cgcagcctct attggttcat
     2101 tttgccagga aggcgaagtt atccgatgag gaaattcggg agctccagga aatccttgcg
     2161 cgcgggagtg gaaaatgagc agcgcgccgg aaggaattgc ggaagccttt gcctgggttt
     2221 tggagcgatc ctggcaggcg acggggctgg tgatttttct gctggttctt cgatgggttt
     2281 ttgcccgggg tttgtccccc tggtggcggc atgcgttatg gctggtgccg gtatgcctcc
     2341 tgattctgcc cgagcttccg gaatgcccgc tcagcgttca ccgctggaag ttggaaaccg
     2401 ttaaaaccgg accgttgatc gcattgccgg tccggagcga gccacccccg gcgattttgc
     2461 cctcaggagg cgaaatgacc ggatttcagg aagagaatcc aggcgcgcct gagccatccg
     2521 ccggatggat gcattgggcg ggtcttggct gggcctcggt tgccgcgggg ttgctcgcgt
     2581 ttttcggagt ggtgaatctg catttccaca tccggttgcg atccaatggc cggccggtcg
     2641 atgcccgggc gctccggatt tttgaggcat gccggcgcga gcttggggtg ggccgaagcg
     2701 gcggggtggt ggagaccgat ctgatcgaca gcccggcaac ggcgggaatt ttccggccga
     2761 gggtgctggt cccgtcgggc ttcggcgaag cattgagcga gatggaaata aggtggatat
     2821 ttttgcatga attgacccac ctcaaacggg gcgacctcct gatgaatgcg gtggtggcgg
     2881 tgctgggagc gctgcattgg tttaatccga ttttggcctg ggccttccgg cagatgcggg
     2941 cggaccggga ggcggcctgc gatgaggcgg tcctcgttca catcggggga gagcaccggc
     3001 aggcctacgg gaaaagcttg ctgctgtgga tgcgcggcgg tctggggaga ttccgcgacg
     3061 ccggattggt gggaatgtcg gcgagccggc gcgaagtcca aagccgcatc gagcaaatcg
     3121 cccgcttcgg cggccggcgt cggggttggt caattgtcag cgccgttttg ttcatcggga
     3181 ttgccgtgac cttcctgacc cgcaccgccg gatcgaatgc cgctctcggg aaggaaattc
     3241 ccgcggcgaa tttcgcgcgg atttgcgatc ggaacggcgt cgttctcgtg gaaatcgagt
     3301 tcaccgcgga cgggaagacg aaggctcgtt atcccttggg cgcgctggcg gcatcgcttt
     3361 tggccgaact ggacgatgcg gccaagccga aggccgggga aagcatttgc ctgacgattg
     3421 atgtccgggc gcaatccgtt atagagaacg aattgcgccg ggtgggacga ggtgccgcag
     3481 ttttgctcga tccttggaat ggggacatcc tggccatggc ctcggtgccg tccttcgatc
     3541 cgaacggcgc atttcccgtg ccgtcgaaaa cggatctttt ggacccgcga atgaatcgcg
     3601 cgcttaccgc ctttactccg ggagcggttt ttctgcccgt gacggcgctg gccgcggccg
     3661 ccgcgggttt ggacaaggag acattcgagt gccgcggctc cctgccggtg ggaaaatata
     3721 cgatgaaatg ctggaaaacc gcggggcatg gccctctggg tttgccggag gcccttaagg
     3781 cttcctgcaa tgtctatttc taccattgcg gcatcgttct gggggagcgg gtgacggaaa
     3841 tggccggtca tttggggttc ggcctcactt cgggaattcc ggtccgcgga gaagccagcg
     3901 gctacatcgg caacccggaa caacccccaa agcgtttccc ctcgcaggtc tggtcggacg
     3961 gatgggcggc caatcgcgcc attggccagg gtgattcgca ggtgacgctc ttgcaggtgg
     4021 cggtcatgat ggggacgatt ggaaatgggg gaaccgttta ttggccgcgc ttggtcaagc
     4081 gtgttatcca gaacggagaa tcccgggagc agccggccgc gcggattcgg gc
//
LOCUS       DATDAY010000298         4707 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_415215, whole genome shotgun sequence.
ACCESSION   DATDAY010000298 DATDAY010000000
VERSION     DATDAY010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 4707)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4707)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4707
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_415215"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..636
                     /gene="gmk"
                     /locus_tag="VIS74_06015"
     CDS             <1..636
                     /gene="gmk"
                     /locus_tag="VIS74_06015"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEY5792835.1"
                     /translation="PTLNSPGRQQFFRKGILFVVSAPSGAGKTTLTTALRQKPDFVYA
                     VSCTTRSPRPGEIHGEDYFFLSREDFKTRLAAGEFLEYAEVHGRFYGTLKQTVLENLD
                     KGVDVLIDVDTQGAAAIRQCPDAFIRESLADVFIMPPDLGELRRRLQKRGTETPEQIQ
                     IRIHNAATEMLSWQEYKYTLISGSMEEDIEKFRAIMRAERYLSRRMIRQLS"
     gene            633..1166
                     /locus_tag="VIS74_06020"
     CDS             633..1166
                     /locus_tag="VIS74_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavoprotein"
                     /protein_id="HEY5792836.1"
                     /translation="MKTIVLGVCGSIAAYKAADLTSKLAKAGHRVFVVMTEHAQEFVG
                     EITFKTLSKNPVTQNLFDEKQSWHPGHIQLADEADLVLIAPATANVLAKLANGIADDA
                     LTSIVLATRAPLLLAPAMNGKMWEHPATRRNVSKLISYGARFIGPEEGLLACGYEGLG
                     RLWDVDGILAEVEKLIG"
     gene            1327..1464
                     /locus_tag="VIS74_06025"
     CDS             1327..1464
                     /locus_tag="VIS74_06025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792837.1"
                     /translation="MPVKKKAPAKAAPAKKAPVKKAPAKKAVAKKPVAKKVVAKKPVK
                     K"
     gene            complement(1569..2564)
                     /locus_tag="VIS74_06030"
     CDS             complement(1569..2564)
                     /locus_tag="VIS74_06030"
                     /EC_number="4.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase family protein"
                     /protein_id="HEY5792838.1"
                     /translation="MIPAASLLDHLPRCPLGCLPTPLAELRRLSRHLGGPRIWIKRDD
                     LTGLALGGNKVRKLEFLFGEARRRGSDCVLTGGATQSNHCRQTAAAAALLGLPCHLAL
                     SGAPPPVPDGNLLLDELLGAQIHWCGEQRKGETLPGIEQELRSRGLNPFVIPYGGSNA
                     TGALGFVAAFQEFSLQAGECQFQPEAIVFASSSGGTQAGLLVGAALAGWPGRLIGIAI
                     DKNATGGEPLPGFITRLAGQAAERLGANLSFDSVILRNEYAGAGYGIVGELEREAIRL
                     LARQEGILLDPVYTGRAFGGLLDLIQRGEFSRGGNVLFWHTGGTPALFPHAADLR"
     gene            2680..3600
                     /locus_tag="VIS74_06035"
     CDS             2680..3600
                     /locus_tag="VIS74_06035"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY5792839.1"
                     /translation="MFERIFFNAAFFCFPIRGVLAALVFAGVVFLAQAEDWQPKTFQV
                     KITGRGPAVLLIPGLACSGDVWNATVAHLQDRYECHVINLKGFGGVPPMEALPAPLLS
                     AVRDEIIAYIRGRGMDHPILIGHSLGGMLALDIAKTAPDLPRRLVVVDSSPFLAGTIQ
                     PEVKPEAVRTIASGLRQKMSGMTREEFEASQRLVIRTLVNAPENGAYLADMANKSDQR
                     TVTEALAELFLADIRPELVKIKCPALVILAVADRVAFRPPEEIEANFRGQYAGLSEVK
                     LLLFEKARHFLMYDEPEKFFAALDAALGGP"
     gene            3747..>4707
                     /locus_tag="VIS74_06040"
     CDS             3747..>4707
                     /locus_tag="VIS74_06040"
                     /inference="COORDINATES: protein motif:HMM:NF013319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HEY5792840.1"
                     /translation="MSVRIFGTHDEATIAQIRRCIAAGGERGVLCADGHKGYAQPIGG
                     VVAYRDLISISGVGFDIACGNLAIRTDAKGGDVRPKIGSIMDDVVRDISFGIGRHNKE
                     RVEHELFEDDLWTEEPFRQFKPMAEAQLGTVGSGNHYVDIFVDEEDRVWVGVHFGSRG
                     LGHKTATCFLKKAGGKDGMDVDPAVVRERDPIGQAYIAGMKLAGRYAYAGREYVARHV
                     VGKILGAKIEEEVHNHHNFAWEEEHGGEKFWVVRKGATPAFPGQKGFVGGSMGDDAVI
                     IEGVDSETSREALYSTVHGAGRIMSRTAAKGKFVKEGGKRIRKE"
BASE COUNT          968 a         1380 c         1479 g          880 t
ORIGIN      
        1 ccaaccttga atagccccgg ccgccagcaa tttttccgca agggaatcct cttcgtcgtt
       61 tccgccccct cgggcgcggg caagacgacg ctcaccacgg ccctgcgcca gaaaccggat
      121 tttgtgtatg cggtttcctg caccacccgc tcgccccggc ccggtgaaat ccacggcgag
      181 gattatttct ttttgagccg ggaggatttc aaaacccggc tcgccgcggg ggaattcctg
      241 gaatatgccg aggtccacgg acgattttac gggacgctca agcaaaccgt gctggagaac
      301 ctcgacaagg gagtggatgt gctcattgac gtggacaccc agggcgccgc ggcgattcgc
      361 caatgcccgg atgccttcat tcgcgagtcg ctggcggatg tcttcatcat gccgcccgat
      421 ctcggcgaac tgcgccggcg cctgcagaaa cggggaaccg aaacgcccga gcaaatccaa
      481 atccgcatcc acaacgccgc caccgaaatg ctgagctggc aggaatacaa atacacgctc
      541 atcagcggct ccatggagga ggacatcgaa aaatttcgcg ccatcatgcg cgccgagcgt
      601 taccttagcc gccggatgat ccggcaattg tcatgaaaac catcgttctc ggagtgtgcg
      661 gctcgatcgc cgcctacaag gcggctgatc tcacgagcaa gctggccaag gccggccatc
      721 gcgtctttgt cgtcatgacc gagcacgcgc aggaatttgt cggcgagatc accttcaaga
      781 cgctttccaa aaatccggtc acccaaaatt tgttcgacga gaaacagtcc tggcatccgg
      841 gacacatcca gctcgccgac gaggcggacc tggtgctgat cgcgcccgcg accgcgaacg
      901 tcctcgccaa gctcgcgaac ggcatcgcgg acgacgcgct cacctccatc gtcctggcca
      961 cgcgcgctcc gctgctcctc gcgccggcga tgaacggcaa aatgtgggag catcccgcca
     1021 cgcggagaaa cgtaagcaag cttatttctt atggcgcgcg cttcatcggg cccgaggaag
     1081 gcctgctggc ctgtggttat gagggtttgg ggcgcttgtg ggatgtggat ggcattctcg
     1141 ccgaggtgga aaagttgatt ggatgatcgc gcggggcgcg gggaaattcc gcgtaataat
     1201 caagcgaaat tcaaaaaaat aattgacgaa agttattcac aaatttattc acaaacatcg
     1261 tgagttactc acacgcacgt ttttctgaat tttcccgcat ctgcggatcg aaagggggga
     1321 aactccatgc ccgttaagaa aaaagctccg gccaaagcgg ctcccgccaa gaaagccccg
     1381 gtgaaaaagg cgcccgcgaa aaaagccgtg gccaagaagc ctgtcgctaa aaaggtggtc
     1441 gctaaaaaac ccgttaaaaa ataatctccg gattccagaa caatctgaga ttttcaaatt
     1501 caaccccgat gagggaagtg acgtttcact tccctcattt tttgtcccct cacgaaagcc
     1561 ggcgaaaatc agcgaagatc ggccgcatgc ggaaacagcg cgggggtgcc gccggtatgc
     1621 cagaacagga cgtttccccc gcgcgaaaac tccccgcgct ggatcaagtc cagcaggccg
     1681 ccgaaggccc ggccggtgta aaccgggtcc agcaggatgc cttcctgcct ggccagcaag
     1741 cggatggctt cccgttcgag ttcccccacg attccgtagc cggcgcccgc gtattcattc
     1801 cgtaaaatca cggaatcgaa ggaaagattg gcgcccagcc gttcggcggc ctgcccggcc
     1861 aggcgggtga tgaagcccgg cagcggctcg ccgccggtgg cgttcttgtc aatcgcgatg
     1921 ccgatcagcc ggccgggcca gccggccagc gcggcgccca ccagcaggcc ggcctgggtg
     1981 ccgccggagg aggaggcgaa gacaatggcc tccggctgga attggcattc cccggcctgg
     2041 aggctgaatt cctggaacgc ggccacgaag cccaatgcgc cggtggcatt ggacccgccg
     2101 tagggaatca cgaatgggtt gaggccccgc gagcggagtt cctgttcgat cccgggcagc
     2161 gtctcaccct tgcgttgctc cccgcaccaa tgaatctggg cgcccagcag ttcgtcgagc
     2221 agcagattgc catccggcac cggcggcggc gcgccgctca gggcgaggtg gcagggcagg
     2281 ccgagcaagg ccgcggcggc ggccgtctgg cggcagtggt tggattgcgt cgcgccgccg
     2341 gtgaggacgc aatcgctgcc ccgccgccgg gcttctccga agaggaattc gagtttgcgg
     2401 actttgtttc cacccaacgc cagaccggtg agatcgtcgc gcttgatcca gattcgcggc
     2461 ccgcccaggt gccgggacag gcgccgtagt tcggccagcg gcgtgggcag gcagcccagc
     2521 ggacaacgcg gaaggtggtc gagaagggag gccgcgggaa tcatgacgaa tccgtttttt
     2581 aatggggttc atccggcagg ggaagcttcc gtttcgcatc cgcggttgct gaactcgaat
     2641 aatggtggaa gggtgacggc gcaatcttgc gaacgattta tgtttgaacg gatttttttc
     2701 aacgcggcgt tcttttgttt tccgattcgg ggcgttctgg ccgcgctcgt tttcgcggga
     2761 gttgtgtttt tggcgcaggc ggaggattgg cagccgaaaa cctttcaagt caaaatcacc
     2821 ggccgggggc ccgcggtgct tttgattccg ggcctggcct gctccggaga cgtttggaat
     2881 gccacggtcg cgcaccttca agaccggtat gaatgccatg tgatcaacct caagggattc
     2941 ggaggggttc cgcccatgga ggctttgccg gcgcccctgc tgtccgccgt gcgcgatgaa
     3001 atcattgcct acattcgcgg gcgcggcatg gatcatccca ttctcattgg gcacagtttg
     3061 ggcgggatgc tggccctgga tatcgcaaaa accgcgcccg atcttccgcg ccgcttggtc
     3121 gtcgtggatt cctcgccttt cctggcgggc acgattcagc cggaggtgaa accggaagcc
     3181 gttcgcacaa ttgccagcgg cctgcggcaa aaaatgtccg gcatgacccg ggaagagttt
     3241 gaagcctcgc aacgcctggt gattcgcacc ctggtgaacg ccccggagaa cggggcgtat
     3301 ctggcggaca tggccaataa gtccgaccag cgcaccgtca ccgaagcgct ggccgaacta
     3361 ttccttgcgg atatccggcc cgaattggtc aaaatcaaat gtcccgcgct tgtcatcctc
     3421 gccgtggcgg atcgcgtagc cttcaggccg ccggaggaga tcgaggcaaa ttttcgggga
     3481 caatatgccg ggctgtccga agtgaagctc ctgcttttcg agaaagcgcg ccacttcctc
     3541 atgtatgacg agccggagaa attctttgcg gcgctggacg ccgccctggg cggcccgtga
     3601 gacggaagcg cgcaatggga aaagcggatg ccggaaaata accggctcca cggcgattgg
     3661 tccggactca gaaatttttg cttccaaatc agtatttcat ccgatcgatt tggcggaatt
     3721 tggaattaaa ccaaggagaa aatatgatga gcgtccgcat ttttggaacg catgacgaag
     3781 cgactatcgc ccaaatccgc cgctgtatcg cggccggtgg agaacggggc gtactctgtg
     3841 ccgatgggca taagggctac gcgcagccga ttggcggcgt cgtggcttat cgcgacttga
     3901 tttcgatctc gggggtcggg ttcgatatcg cctgcggcaa cctggccatc cgcacggatg
     3961 ccaagggcgg cgatgtccgc ccgaagatcg gatcgatcat ggatgatgtc gttcgcgaca
     4021 tttcgtttgg gatcggccgc cataacaagg agcgcgtcga acacgaattg ttcgaggacg
     4081 atctttggac cgaggagccg ttcaggcagt tcaagccgat ggcggaggcc cagcttggaa
     4141 ccgtgggaag cggcaaccat tacgtggata tcttcgtcga tgaagaagac cgcgtgtggg
     4201 tgggcgttca tttcggttcg cgcggcctcg gccacaagac ggccacctgc tttctcaaga
     4261 aggcgggcgg caaggacggg atggatgtgg atccggccgt tgtgcgcgaa cgcgacccca
     4321 ttggccaggc ctatatcgcc ggcatgaagc tggcggggcg ttacgcctac gcgggccgcg
     4381 aatacgtggc gcgccacgtc gtgggcaaga tcctcggggc gaagatcgag gaagaggtgc
     4441 acaaccacca caacttcgcg tgggaggagg agcatggcgg cgagaagttc tgggtggtcc
     4501 gcaaaggggc gactccggcc tttcccggcc agaagggctt tgtcggcgga agcatgggag
     4561 acgatgccgt catcatcgag ggcgtcgatt ccgaaacttc ccgcgaggcc ctgtattcca
     4621 ccgtccacgg agccggccgc atcatgtcgc gcacggcggc caagggaaag ttcgtcaagg
     4681 aaggcggcaa gcgcatccgc aaggagg
//
LOCUS       DATDAY010000299         2267 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_556731, whole genome shotgun sequence.
ACCESSION   DATDAY010000299 DATDAY010000000
VERSION     DATDAY010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2267)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2267)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2267
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_556731"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2267)
                     /locus_tag="VIS74_06045"
     CDS             complement(<1..>2267)
                     /locus_tag="VIS74_06045"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY5792841.1"
                     /translation="PLVREDRPLDKVTLVAMGSPEIAEAIGREIAATGSPAALLSGAT
                     LLDPEALRNAVTEIRAFQGPIGALVYVGGIGTGAMPDTLADWRQACQVDAKALFVLLQ
                     ACAPDLQESKGRVLSLSAMGGGFGRRPSPWHSLPSAGAPSGLLKTAAIEWPDVRFRVI
                     DFAEPDADLVGQAVAGELLAADMATEIGYFDGARQVYFEYSAPQPGDEKPRLEAGPDW
                     VLLVTGGARGITAEVLLGVLKPGMTVHLIGRAEEPEAEAGWSLGVPAEKLKKIIIDRM
                     KAVGETLTPALVEKAISAVLRDREIRDNLESFRSRGATVVYHSADVRDEAQMRGILDG
                     IYGRHNRIDMVIHGAGVIEDKLLVDKTMESFDRVFDTKADSTYLLSRLLRPESLRCLA
                     LFASVAGRTGNRGQCDYAAANELVNRFGWWFRAHWPHVRVSSINWGPWESGMASAEVN
                     RQFRERGVIPIPPKQGREFFRREIFCCPPGEEEIVAGIFLKPVSAARPPAWPLLPTAT
                     IRHEEDQVTAEWQLSVGDVPALDDHRIDGKAIVPWACALEFMAQTVQSTWPDWHVIEA
                     LNHQQLRGITVEEDGRFPLRVTAKAREEDGALLVSAMAFEIGSPPRPCYRSEFRLLRE
                     PPDAPFAPSVPKCAPASVTPRVFYTEYAFHGPGFRLLELIDGLDASGLDAAIRPTGIW
                     RDAPWVFHPGLMDMALQAGSFWAQWQLRSFGLPTKAVRIVRYGPHRFGTGGLRLAARV
                     TETTNQTITFDFFIA"
BASE COUNT          424 a          742 c          720 g          381 t
ORIGIN      
        1 cgcgatgaaa aaatcgaaag tgatcgtctg gttcgtcgtt tccgtcaccc gcgcggccag
       61 gcgaagcccg ccggtgccga atcggtgcgg accataacgc acgatgcgga cggccttggt
      121 cggcaggccg aaactgcgaa gctgccactg cgcccaaaaa cttcccgcct gcaaggccat
      181 gtccatgaga ccggggtgga acacccatgg ggcgtcccgc cagattcccg tgggccggat
      241 ggccgcgtcg aggccggagg cgtcgagacc gtcgatgagc tcaaggaggc ggaagcccgg
      301 accgtgaaaa gcatattcgg tgtaaaaaac ccggggcgtc acggacgccg gggcgcactt
      361 ggggacggaa ggcgcgaacg gcgcatccgg cggctcgcgc agaaggcgaa actccgagcg
      421 atagcagggg cgcggtggcg aaccgatttc gaatgccatg gcactcacga ggagtgcacc
      481 gtcttcttcc ctggctttgg cggtgacgcg cagagggaac ctcccgtcct cctcgaccgt
      541 gatgccgcgg agctgttgat gattgagcgc ctcgatcaca tgccagtccg gccaggtact
      601 ttgtacagtt tgggccatga actccaatgc gcaggcccaa ggtacgatgg cctttccgtc
      661 aatccggtgg tcgtccagcg caggcacgtc gcccaccgag agctgccact cggcggtcac
      721 ctgatcctcc tcgtgccgga tcgtggcggt cgggagaagc ggccaggccg gaggcctggc
      781 ggcggacact ggtttgagaa aaatcccggc gacgatttcc tcctcgcccg gcgggcagca
      841 gaagatttcg cggcggaaga actcccggcc ttgcttcggc gggatgggga tcacgccgcg
      901 ttcacggaac tggcggttca cttccgccga ggccattccg ctttcccaag gcccccaatt
      961 gatcgagctc acacgcacgt gcggccaatg agcccggaac caccagccga agcggttcac
     1021 caattcgttg gccgccgcgt agtcgcattg gccgcgattg cccgtgcggc cggccaccga
     1081 agcaaaaagg gccaggcaac gaagcgactc gggccggagc agacggctga gcaggtatgt
     1141 gctgtcggcc ttggtgtcaa aaacgcgatc gaaggattcc atcgtcttgt ccaccaggag
     1201 cttatcctca atgactcccg cgccatggat gaccatgtcg atgcggttgt gccggccgta
     1261 gatgccgtcg agaatcccgc gcatctgtgc ctcgtcccgc acgtcggccg aatgataaac
     1321 aaccgtggct ccacgcgaac ggaagctctc caaattatcc cggatctcgc ggtcgcgcag
     1381 aaccgcggag atggcttttt ccaccagggc gggcgtgagt gtctcgccga ccgccttcat
     1441 ccggtcgatg atgatttttt tgagtttttc ggccggtacg ccgaggctcc atccggcttc
     1501 cgcttccggc tcttcggcgc gcccgatgag gtgcacggtc atacccggtt taaggacccc
     1561 caggaggacc tccgccgtaa tcccccgggc gcctcccgtg accaaaagca cccagtccgg
     1621 accggcttcc agccggggtt tctcgtcccc gggctgcggc gcggagtatt cgaaatacac
     1681 ctgccgcgcg ccgtcaaaat atccgatctc ggtcgccata tcggcggcca gcagttcccc
     1741 ggccacggcc tggccgacca aatccgcatc aggttcggca aagtcgatca cgcgaaaacg
     1801 gacatccggc cattcgatgg cggcggtttt aaggagacct gagggagcgc ctgccgaggg
     1861 aaggctgtgc catggggaag gacggcgtcc gaaacctcct cccatcgcac taagggagag
     1921 cacccggccc ttggactcct ggaggtctgg cgcgcaggcc tggaggagaa caaacaatgc
     1981 cttggcgtcc acctggcagg cttgccgcca gtccgcgagg gtgtccggca tcgcgccggt
     2041 cccaatcccg ccgacataaa ccaatgcgcc gatcggcccc tgaaaggcgc ggatctcggt
     2101 caccgcgttc cgcaatgcct ccgggtcgag cagggtcgcg cccgacagca aggcggccgg
     2161 gctgccggtc gcggcgatct cccgcccgat ggcttcggca atttccggcg aacccatcgc
     2221 aacaagggtg actttgtcca gaggcctgtc ctccctcacc agcggag
//
LOCUS       DATDAY010000300         3276 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_654064, whole genome shotgun sequence.
ACCESSION   DATDAY010000300 DATDAY010000000
VERSION     DATDAY010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 3276)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3276)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3276
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_654064"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..421
                     /locus_tag="VIS74_06050"
     CDS             <1..421
                     /locus_tag="VIS74_06050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792842.1"
                     /translation="VVTEGNLFIDAEAQIRGEAPAAASPAPPAEAVPPDKIAQMAAAL
                     AEDDLPAFVKTLEPGQPVPATLAEARRQFLPLANARAAAALQSRSKDFTVFECPMTGS
                     AFPGAPASARWIQAAGTELRNPYLGREMQSCGNEVKP"
     gene            418..>3276
                     /locus_tag="VIS74_06055"
     CDS             418..>3276
                     /locus_tag="VIS74_06055"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HEY5792843.1"
                     /translation="MIERIIDWSLNHRFLVLGATLLLAALGLQALRDTPVDAIPDLSE
                     NQVIVFADWPGRSPQEVEDQVTFPLSTNLQGLAGVKEVRGTSMFGFSLLTVIFEDKVD
                     NYFARSRVLERLSYLQSLMPEKVAPQLGPDATGLGWVYQYYLKVDPDRAATNLGDLRA
                     LQDFFVRYQLAAVEGVAEVASIGGFVKTYVVNVSPERMRQSGVTMAEVMAALGNANLN
                     VGGKTVQENGMEFIVRGVGLIQSLADLEKVSVGLRDGRPVYLKDFATLSLGGDYRRGA
                     LDVNGQEAVGGMVIMRTGENAKQVIERVKARIARIQGSLPPGVSIEPVYDRSDLIERT
                     LETLRHALWEEIALVILAHILFLWHFRSILIVTLPLPVSILISFILMRGFGITSNIMS
                     LAGIAIAIGVLVDAGIVMTENIIRQCEKAANAGDPRPRLEIIRAAAKQVGRPIFFAMA
                     IIILAFLPVFALGGQEGKLFHPLAFTKTFAMAGATLLAVTFVPALCVWLVRGPFHPEE
                     ENPLMAFLRRIYTPALDWALRHGRVVIGAAVLLLAGTGFLASRMGSEFMPPLNEGSLL
                     FMPVLLPSTSFNEVQRILSWQDQVLKSVPEVVSAAGKLGRADTATDPAPVEMIETTIL
                     LKPPKEWRPGLTKEKLIAELSEKLSQIPGYVPGFLQPIENRLLMLSTGIRAQVGVKLL
                     GADLDALQKKAFEVERVIRAIPGAAGVAPSRVQGKPYLEVEPDRVALARYGLSLREVM
                     DIVEAGLGGLNVSATIEGRQRFPIQVRLPASEREDLSRLGDILIPLGGGKSVPLGQVA
                     KIRRTTGPNMIESEDGLLRVYVQTNVTGRDLGGFVQEIQDRIAREVDLGPGVALKISG
                     QYEHQLSAQRTLSFIVPIVLLIIFTLLCVVFRSALEAAHVILAVPFALTGGVILQWLL
                     HYPFSVAVWVGYIALFGTAIQTGVVMVVYLEEAVARKRA"
BASE COUNT          537 a         1136 c         1037 g          566 t
ORIGIN      
        1 ggtcgtcacc gaaggcaatc tgttcatcga tgccgaagcg cagattcgcg gggaagcccc
       61 cgctgccgcc tcccccgctc cgcctgccga ggcggtcccg cccgataaaa tcgcccaaat
      121 ggccgccgcg ctggcggagg acgatctccc cgcctttgta aaaaccctcg agcccggcca
      181 gccggttccg gccacgctgg cggaggcgcg gcggcaattc ctcccgctcg ccaacgcccg
      241 cgcggcggcg gcgctccagt cgcgttcgaa ggatttcacc gtttttgaat gcccgatgac
      301 cggttccgcc ttccccggcg cgccggccag cgcccgctgg atccaggccg ccggaaccga
      361 attgcgcaat ccctatctcg gacgcgaaat gcagtcctgc ggaaacgagg tgaagccatg
      421 atcgagcgaa tcattgactg gtcgctgaat caccgcttcc tcgttctcgg ggccacgctg
      481 ctcctcgcgg cgctgggcct gcaggcgctg cgcgacacgc cggtggacgc cattccggac
      541 ctgagcgaaa atcaggtcat cgtcttcgcc gactggccgg ggcgcagccc gcaggaggtg
      601 gaggatcagg tgacgtttcc gctttcgacc aacctgcagg ggctggccgg ggtgaaggaa
      661 gtccgcggca cctccatgtt cggcttctcg ctgctcacgg tcatcttcga ggacaaggtg
      721 gacaattatt tcgcccgctc ccgggtgctg gaacggctca gctatttgca atccctgatg
      781 cccgaaaagg tggcgccgca attgggcccc gacgccacgg ggttgggctg ggtgtatcaa
      841 tattacctca aggtggaccc ggatcgcgcc gccacgaacc tcggcgacct gcgcgcgctg
      901 caggacttct tcgtgcgcta ccagctcgcc gccgtcgagg gcgtggccga agtcgccagc
      961 attggcgggt tcgtgaagac ctacgtcgtc aacgtctcgc ccgagcggat gcgccagagc
     1021 ggcgtcacca tggccgaggt catggccgcc ctgggcaacg ccaacctcaa cgtcggcggc
     1081 aagaccgtgc aggaaaacgg catggagttc atcgtccgcg gggtcggcct gatccagtcg
     1141 ctggccgacc tggaaaaggt ctccgtcggg ctgcgggacg ggcgcccggt gtatctgaag
     1201 gatttcgcca cgctttccct cggcggcgac taccggcgcg gcgcgctgga cgtcaacggc
     1261 caggaggcgg tcggcggcat ggtgatcatg cgcacgggcg aaaacgccaa gcaggtcatc
     1321 gaacgcgtga aggcgcgcat cgcccgcatc cagggcagcc tgccgccggg cgtgagcatc
     1381 gaaccggtct atgaccgcag cgatctcatc gaacgcacgc tggagaccct ccgccacgcg
     1441 ctgtgggagg aaatcgcgct cgtcatcctg gcgcacattc ttttcctctg gcacttccgc
     1501 agcatcctca tcgtcaccct gccgctgccg gtttcgatcc tgatctcctt catcctcatg
     1561 cgcggtttcg gcatcacgtc gaacatcatg tcgctggccg gcatcgccat cgccatcggc
     1621 gtgctcgtgg atgcgggcat cgtcatgacg gaaaatatca tccgccaatg cgaaaaggcc
     1681 gcgaacgccg gcgatccgcg cccccgcctc gaaatcatcc gggccgccgc caagcaggtc
     1741 ggacggccca ttttcttcgc gatggccatc atcattctcg ccttcctgcc ggtcttcgcg
     1801 ctcggcggcc aggaggggaa actcttccat cccctcgcct tcaccaagac cttcgccatg
     1861 gccggcgcca cgctgctcgc cgtgaccttc gtgcccgcgc tgtgcgtctg gctcgtgcgc
     1921 ggcccgtttc atccggagga ggaaaatccc ttgatggcct tcctgcggcg gatctacacc
     1981 ccggcgctgg attgggcgct gcggcacggg cgcgtggtca tcggcgcggc cgtgctgctg
     2041 ctcgccggca cgggttttct cgccagccgg atgggcagcg aattcatgcc gccgctcaac
     2101 gagggcagcc tgctcttcat gcccgtgctg ctgcccagca cgtcgttcaa cgaggtccag
     2161 cgcatcctgt cctggcagga tcaggtcctc aaaagcgtgc cggaagtcgt ctccgccgcc
     2221 ggcaagctcg gccgcgcgga caccgccacg gaccccgcgc cggtggagat gatcgagacc
     2281 acgatcctgc tcaagccgcc caaggaatgg cggcccggcc tcaccaagga aaagctcatc
     2341 gccgagctca gcgaaaagtt gtcgcagatt cccggctacg tgcccggatt tctccagccg
     2401 atcgaaaacc gcctgctcat gctcagcacg ggcatccgcg cgcaggtcgg cgtgaagctg
     2461 ctcggcgccg atctggacgc gctgcaaaaa aaggccttcg aggtcgagcg cgtcatccgc
     2521 gcgattcccg gagccgccgg cgtggcgcct tcgcgcgtgc agggaaaacc ctacctcgag
     2581 gtcgaaccgg accgcgtggc gctcgcccgc tacggcctga gcctgcgcga agtcatggac
     2641 atcgtcgagg ccggcctggg cgggctcaat gtcagtgcga cgatcgaagg ccgccagcgc
     2701 tttccgatcc aggtgcggct gcccgccagc gaacgcgagg atctttcccg gcttggcgac
     2761 atcctgattc cgctgggcgg cggcaaatcc gtcccgctcg gccaggtggc gaaaatccgg
     2821 cgcacgaccg gaccgaacat gatcgaaagc gaggacgggc tgctgcgcgt gtatgtgcag
     2881 acaaacgtga ccgggcgcga cctcggcggg ttcgtccagg aaatccagga tcgcatcgcc
     2941 cgcgaggtgg acctcgggcc gggggtcgcc ctgaaaatca gcgggcaata cgaacaccag
     3001 ctcagcgccc agcgcacgct cagcttcatc gtgccgatcg tgctgctcat catcttcacg
     3061 ctgctgtgcg tggtgttccg ctccgcgctc gaggcggcgc acgtcatcct ggccgttccc
     3121 ttcgcgctca ccggcggagt catcctgcaa tggctgctcc attacccctt cagcgtcgcg
     3181 gtgtgggtcg gttacatcgc cctcttcggc acggcgatcc agacgggcgt ggtgatggtc
     3241 gtctatctgg aggaagccgt cgcccgcaag cgcgcc
//
LOCUS       DATDAY010000301         3365 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_2044949, whole genome shotgun sequence.
ACCESSION   DATDAY010000301 DATDAY010000000
VERSION     DATDAY010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 3365)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3365)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3365
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2044949"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1844)
                     /locus_tag="VIS74_06060"
     CDS             complement(<1..1844)
                     /locus_tag="VIS74_06060"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY5792844.1"
                     /translation="MRALIFLLLCGPLALAAEPVLERARETLAKGWSSAVLLELDDKT
                     LNALPADSQREALLLRARAQLQSGNPEAAQTTLRDARLADFPAARLLRGDAFFAEKNY
                     ESARRAYAEAGGGPAALGEARSLRALGRGQEAVAVLSAIPLKSEAGRDAALERAAIWL
                     EWKKDSPVRELVPQMEAAFPGPDARIQYLRGVLDWRAGKLEAARASLSEAARDEGETG
                     ALAVLALSEVEAASGDPAGAEDVLEDYLRRSQGSRLLLRVLENLDALYERGSSWSSDL
                     RAVAEDQSNPVRARMAGYFLARNEARLGRTDTAKRTYEKFIANSPDEALKQRAVEEYA
                     DLLIRTQAFDPAVALLQDPAVARNPRMRIKLAEVFAAQGRYPEAEKEYLTAAGDASLG
                     EAAAANAAYCALLAGQPAAENAGLRILRETHPSSPRIAEVELWLARDAARCRGAEAPA
                     QLQQLARTGNPEAALHLAEWRFIENDPAESARELARANLSGADGERAAYLAVFLTDQG
                     SEENSDRALLLAEDFIKRYPQSAYLPEVEFKRAEVLFRSGDYQTARMAFAKLAADYPA
                     SPLAGRAWLQAGLAGMKLMNRPALERSREDLEEAAKSDPSLAALARFHR"
     gene            complement(1841..>3365)
                     /gene="uvrA"
                     /locus_tag="VIS74_06065"
     CDS             complement(1841..>3365)
                     /gene="uvrA"
                     /locus_tag="VIS74_06065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEY5792845.1"
                     /translation="DQLELTESQQLVVDDVRREIRQRLVFLDEVGLGYLNLNRESGTL
                     SGGESQRIRLASQIGSGLAGVLYVLDEPSIGLHQRDNDRLIATLRGLRDLGNSVIVVE
                     HDEDTIRAADFILDLGPGAGPRGGRLVAHGTLDEILKSEESLTAQYLTGGLKIPVPRL
                     RLPPRKDKPKKPEPGDGWVTVLGARENNLQNLDVSFPVGCFTGVTGISGSGKSTLVND
                     ILRAVLARDLNGAKEKPGAHRAVIGTEQLDKVIVIDQSPIGRTPRSNPATYSGVLGPI
                     RELFAQVPSARVRGYDAGRFSFNIKGGRCEHCQGDGLIKVEMHFLADVYVECEVCRGR
                     RFNRQTLEITYKGKNIADVLEMTVDEASRFFRSIPVLADKFDSLLQVGLGYLRLGQPA
                     NTLSGGEAQRLKLASELARRSTGRTAYLLDEPTTGLHFADIHKLLEVLLRLRDSGNTL
                     IVIEHNLEVIKCADWIIDLGPEGGEGGGRIVAEGTPEEIAACGQSHTGAYLKAILQK"
BASE COUNT          540 a         1178 c         1088 g          559 t
ORIGIN      
        1 cgatggaaac gcgcgagcgc cgccagggac ggatcgctct tggccgcctc ctccagatcc
       61 tcccgcgacc gctccagcgc gggacgattc atcagcttca tgcccgccag ccccgcctgc
      121 agccaggccc ggcccgccaa gggcgaggcg ggatagtccg ccgcgagctt ggcaaaggcc
      181 attcgcgccg tttgataatc cccgctgcgg aacaaaacct ccgcccgctt gaattccacc
      241 tcgggcagat acgcggactg cggataacgc ttgataaaat cctcggccag gagaagcgcg
      301 cggtcggaat tttcctcgct gccctggtcc gtcagaaaaa ccgcgaggta agccgcccgc
      361 tcgccatccg cgccgctcag attcgcccgg gcgagttcgc gggcggactc cgccggatcg
      421 ttctcaatga agcgccactc ggccaggtgc aaggcggctt cgggattccc ggtccgggcc
      481 agctgctgaa gctgagccgg cgcctccgcg ccgcggcagc gcgccgcatc ccgcgccagc
      541 caaagctcga cttcggcgat gcgcggactg gagggatgcg tttcccgcaa gatccgcagg
      601 ccggcatttt ccgcggcggg ctggcccgcg agcagggcgc agtaggcggc attggccgcg
      661 gcggcctcgc ccagggaggc atccccggcg gcggtcagat attccttttc cgcctccggg
      721 tagcgcccct gcgcggcgaa gacctccgcg agcttgattc gcatccgcgg atttcgggca
      781 accgccggat cctgcagcag ggcaaccgcc ggatcgaagg cctgtgtccg gatgagcaga
      841 tcggcatatt cctccaccgc gcgctgcttc aatgcctcat cgggggaatt ggcgatgaat
      901 ttttcgtagg tccgcttcgc ggtgtccgtc cggcccagcc gggcctcgtt ccgcgccagg
      961 aaataacccg ccattcgggc gcggacggga ttcgattgat cctccgccac ggcgcgcaaa
     1021 tccgaactcc agctcgaccc gcgctcgtaa agcgcgtcga gattctccag cacgcgcagc
     1081 agcagccgcg acccctggga gcgccgcaaa taatcctcga gcacgtcctc cgcgccggcg
     1141 gggtcgccgg aagccgcctc cacctccgag agcgcgagga ccgccaaagc cccggtttcg
     1201 ccctcgtccc gggccgcctc gctcagggac gcgcgggcgg cttcaagttt ccccgcccgc
     1261 cagtccagga cgccccgcag gtattgaatc cgcgcatccg gtccgggaaa ggcggcttcc
     1321 atttgcggca cgagctcgcg caccggggaa tcctttttcc attccagcca gatggccgcc
     1381 cgctccagag cggcatcccg gcccgcctcc gacttcaggg gaatggccga taaaaccgcc
     1441 accgcctcct ggccgcgtcc aagcgcgcgc aggctccgcg cctcgcccag cgcggccggc
     1501 cccccgcccg cctccgcgta ggcccggcgg gcggattcgt aatttttttc cgcaaaaaag
     1561 gcatcgcccc gcaaaagccg cgcggcgggg aaatccgcca gccgcgcgtc ccgcagcgtc
     1621 gtttgggcgg cttccggatt gccggattgc agctgcgcgc gggcgcggag cagcagcgcc
     1681 tcccgttgcg aatcggcggg cagggcgttc agggttttat catccagctc cagcagcacc
     1741 gcgctcgacc agcccttggc cagcgtttcc cgcgcgcgct ccagcaccgg ctccgcggcc
     1801 agggcaagcg gcccgcacag gaggaggaaa atcagggctc tcatttctgt agaatggctt
     1861 tcaaataagc tccggtatgg ctttggccgc aagccgcgat ttcctcggga gtgccttccg
     1921 cgacgatgcg cccgccaccc tcgccgccct cggggccgag gtcgatgatc caatccgcgc
     1981 acttgatgac ctcgagattg tgttcgatga cgatgagcgt attccccgaa tcgcgcaggc
     2041 gcaggagcac ttccagcagc ttgtggatat ccgcgaaatg gaggccggtc gtcggttcat
     2101 ccaataaata cgccgtccgc cccgtgcttc gccgggcgag ctcggacgcc agcttgagcc
     2161 gctgggcctc gccgccggag agcgtgttcg ccggctggcc gaggcgcagg taaccgaggc
     2221 cgacctgcag caggctgtcg aatttgtcgg ccagcacggg aatgctgcga aagaaccgcg
     2281 acgcctcgtc cacggtcatt tccagcacgt cggcgatgtt ttttcccttg taggtgatct
     2341 cgagcgtctg ccggttgaac cgccggccgc ggcagacctc gcactccaca tacacgtcgg
     2401 cgaggaaatg catctcgacc ttgatgagcc catccccctg gcaatgctcg cagcgcccgc
     2461 ccttgatgtt gaaggagaac cgtccggcgt cgtaaccgcg gacgcgcgcg gagggcacct
     2521 gggcgaagag ctcgcggatc ggcccgagca cgccggagta ggtggcggga ttcgagcgcg
     2581 gggtgcggcc gatcggcgac tggtcaatga cgatcacctt gtcgagctgc tcggtgccga
     2641 tcacggcgcg gtgcgcgccg ggtttttcct tcgcgccatt cagatcgcgg gccagcacgg
     2701 cgcggaggat gtcgttcacg agcgtggatt tgccgctgcc ggaaatgccg gtcacaccgg
     2761 tgaagcagcc gaccggaaac gacacgtcga ggttttgcag attgttttcc cgcgcaccca
     2821 gcacggtcac ccagccgtcg cccggttcgg gtttcttcgg cttgtccttg cgcggcggca
     2881 ggcgcaggcg cggcaccggg atttttaacc ccccggtcag gtattgggcg gtcagggatt
     2941 cctcgctttt caggatctcg tccagcgtcc cgtgcgcgac cagccgcccg ccgcgcgggc
     3001 ccgctccggg gccgaggtcg agaatgaaat ccgccgcgcg gatcgtgtcc tcgtcgtgct
     3061 cgacgacgat gacggaattc cccaggtcgc gcaggccgcg gagcgtggcg atcaggcggt
     3121 cgttgtcgcg ctggtgcagg ccgatgctgg gctcgtcgag cacatagagg acgcccgcca
     3181 gcccggagcc gatctggctc gcgaggcgaa tgcgctggga ttcgccgccg gagagcgtgc
     3241 cgctttcgcg gttgagattc aaataaccca ggccgacctc gtcgagaaag acgagccgct
     3301 gccggatttc ccgccgcaca tcgtccacca ccagctgctg gctttcggtg agttccagct
     3361 gatca
//
LOCUS       DATDAY010000302         2502 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_1867330, whole genome shotgun sequence.
ACCESSION   DATDAY010000302 DATDAY010000000
VERSION     DATDAY010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2502)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2502)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2502
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1867330"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..586)
                     /locus_tag="VIS74_06070"
     CDS             complement(<1..586)
                     /locus_tag="VIS74_06070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792846.1"
                     /translation="MSGKAPAGQVVAVTFQGQTVTTGADANGAWKINLPPVTVPGGPF
                     PMIISSGSEQLTLAYVLIGDIWFCSGQSNMEVPLRVMAPEEVGLATVPGLRLLKVPHQ
                     ASSAPVAARPASWRACTPSTISEFSALAYFFGRDVNRETGVPVGLIMATWGGKRAEVF
                     TRREILLADPVLKPIVDRWDALMAAYPEALSIYEI"
     gene            complement(1534..>2502)
                     /locus_tag="VIS74_06075"
     CDS             complement(1534..>2502)
                     /locus_tag="VIS74_06075"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY5792847.1"
                     /translation="RFGIAVMRFLHTLLSMLASLRVFLIALEEGSLNRAAARLRMSQS
                     ALSRQMQALEAEIGGALLERTTSGVRATDAGYALANSLPPILAEYDAAFNEARRLARG
                     QRDLLRIGYLGSAAQRFLNPALSHLRQAHPEVKVKLLDLSPGEQIAALRRGEIDLAVT
                     GQEAGLGSSEFYTRKLATLPVVAAMAADHPLAGEKRIELRQLRGERFLGTPEEDLPGR
                     DRWIVQLCRRAGFRPNFVQRADTLSQALSLISSEGTVALLPGYLKTYPAAGVSMVELS
                     DPRATWDFLVVWQRGKMPDALKDLLEALTAEAQKPEGRPAWNSANV"
BASE COUNT          534 a          747 c          753 g          468 t
ORIGIN      
        1 cgatttcata tatgctcaaa gcctccggat acgcggccat gagggcatcc cagcggtcca
       61 cgatcggttt caggaccggg tcggccagca gaatttcacg ccgcgtgaaa acttcggctc
      121 gtttgcctcc ccaggtcgcc atgatcagcc cgaccggcac tccggtttcc cggttgacat
      181 cgcgtccgaa gaaatacgcc agcgccgaga actccgaaat ggtggagggc gtgcacgccc
      241 tccatgaggc aggacgcgcc gccacgggcg cgctggacgc ctggtgcgga accttgagca
      301 ggcgcaagcc cggcacggtg gccaagccta cttcctcggg cgccatcacg cgcaggggaa
      361 cctccatgtt cgactgcccc gagcagaacc aaatgtcccc gatgagcaca tacgcaagcg
      421 ttaattgctc ggagccgctc gaaatgatca tcggaaacgg gccgcccggc accgtcaccg
      481 gcggcagatt gatcttccag gcgccattgg catcggcccc cgtggtcacg gtttgcccct
      541 ggaaagtcac cgccaccacc tggccagccg gagcctttcc ggacaacgaa tttacggtat
      601 ttcgctgaag aatcatcctc gcgctcgcgg gaggggaaac cgaaataccc gccactgcca
      661 aaaaaataga cgaaaattcc gcgacacagg tgacgaagag gaaccagaga atcttgcgcg
      721 agaggggaag gggggagagt ttcatatgat tagatgctaa aatatcgtgc ggaggggcca
      781 gggcagacgt atcaaaatcc gaggagggat agccgtctct taagatttac ataagaatct
      841 agagcgtgtt attaactttt gggaaattag cttgtaactg cctatatatc aaaatattac
      901 attctatcaa agtcttgcgt tttcgccgaa atcttagtcg taactcgctg attgccaagg
      961 tgcattttga aagttcataa cacgctctaa taataaaata attatggctt gataattact
     1021 gtctggttta cggagactcc cccagagtcc ttcatttttc cggtaaaccg agcgatgaaa
     1081 aatcgtcctg ccccgagtca ccagagttcg tttttgctgc ccgatctgag cagccagctt
     1141 gatccccgtc atgagcttat gccgattggc ggctcgattg aatttggacc aattcgaagg
     1201 agccttcggg gctctttaca gcgacgaagg acggccgggt ttgccgatcc ggcggatggt
     1261 gggtctggcc ctggggcgcc agcaaatcag ccttcggtta cttcctgcgg ctggggggta
     1321 gatttagcct cgggcggccg cggcggcaga caaaaaggtt tgtgagcaag caacttggcg
     1381 gtttatggca gcgcgcgggc gctcatccgc cggacggatg gcggacgctt ctgaaccccg
     1441 cgaaggactg aaggaagacg gaatgaaaca gaaggccaca aaggaaggag atcggaaaat
     1501 gttccagtct ttgtgctctt tgtgtccttc tgttcaaaca ttggccgaat tccacgctgg
     1561 ccgcccttcc ggcttttggg cttcggccgt cagggcttcc agcaaatcct tgagcgcgtc
     1621 cggcattttt ccccgctgcc agaccacgag gaaatcccag gtggcccgcg ggtcggaaag
     1681 ctccaccatg ctcaccccgg cggcgggata cgtttttaag taacccggca gcaaggcgac
     1741 ggttccctcg ctgctgatca gcgagagcgc ctgcgacaag gtgtccgccc gctggacaaa
     1801 attgggccgg aacccggcgc ggcggcagag ctggacgatc cagcgatccc ggcccggcaa
     1861 atcctcctcg ggcgtgccga gaaaccgttc gccgcgcaac tggcggagtt cgatgcgttt
     1921 ctcccccgcc aggggatgat cggcggccat ggccgccacc acgggcagcg tcgccagctt
     1981 gcgcgtgtaa aattcgctcg atcccagccc ggcttcctgg ccggtgacgg cgagatcaat
     2041 ctccccgcgg cgcagggcgg caatctgctc gccgggggag agatcgagca gtttgacctt
     2101 cacctcgggg tgcgcctggc gcagatggga aagcgccggg ttgagaaacc gctgcgccgc
     2161 ggagcccagg tagccgatgc gcaataaatc ccgctggccg cgcgccaggc ggcgggcttc
     2221 gttgaacgcc gcgtcgtatt ccgcgaggat cggcggcagc gaattcgcca gcgcgtagcc
     2281 agcgtcggtc gcgcgcacgc ccgaggtcgt ccgctccagc agcgcgcccc cgatctcggc
     2341 ttcaagcgcc tgcatctggc gggacagggc ggattgcgac atccgcagcc gcgcggcggc
     2401 gcggttgagg ctcccctcct ccagcgcgat caggaaaacc cggagcgatg ccagcatgga
     2461 aagcaaggta tgcaaaaaac gcataacggc aatcccaaac cg
//
LOCUS       DATDAY010000303         3882 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_1561776, whole genome shotgun sequence.
ACCESSION   DATDAY010000303 DATDAY010000000
VERSION     DATDAY010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 3882)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3882)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3882
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1561776"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2198
                     /locus_tag="VIS74_06080"
     CDS             <1..2198
                     /locus_tag="VIS74_06080"
                     /inference="COORDINATES: protein motif:HMM:NF013652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY5792848.1"
                     /translation="LPGEYLEMLFEIAVEEAERLSWVLLADFGKILPESWLKVAQAAG
                     AAKRDQLTAEILKRLSEPEILEKFSEEQAKEVKWMGDMIAHGKRSEAPDPDKISTISL
                     GVIDYRSIDYVRASKNIGDFVQTVACLSNLARFRNTRFSGDEGLAQFVTGLKDRVYPD
                     RHIEGPPVDINLVATNRDYTSRSGAPAGTWLIAFGWHMHSLFNAAYDFPFDDSLRPIF
                     FSFHINNPDLLTDEVIAYLKSKAPIGCRDWNTVDMLFSHGVDAFFSGCLTTTVDNILR
                     GSRKSPSESAWKVAFVNVPPAPEEKATPYDTINIMKPEILERTFSENLAAAVEQLEEY
                     ARYDLLVSSKLHCYLPSTAMGLKVDFRPKRPADIRFEGLYGLDPKTIETVRQPILRKL
                     QLIFEKIFTGAAEEEVYSYWREVCAPDVERARAYCQPPPPAPPSFDIQVAADRVLSAW
                     PSDQAGEANGDYIEVAFSTDENLKDQLLVVLESAVAHTSRPIRAWILSRGLGPDDFAR
                     YEKLFPQIRFRFLPCDSVDYGQILGLLIHTTVATMDRLLLPVLVPAKKMIYLDIDLLV
                     RADLAELFDIPMGESPLAGRTSMMESWSTGYHLVYRSANRLDAARSIELRRRMHALHD
                     LRFPGFNAGVLLLNLDQMRKDRFCEEYLPFAERYGMNDQEVLNCYAGDRRIELDLNWN
                     CLAAQDMIDHAKIIHWAGHVKAWNCNWVVGKNYWDYHEAKVRARLAQES"
     gene            2195..>3882
                     /locus_tag="VIS74_06085"
     CDS             2195..>3882
                     /locus_tag="VIS74_06085"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016145.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase family
                     protein"
                     /protein_id="HEY5792849.1"
                     /translation="MNGILISFYSRDPYYQGCAEKLREDCIRLKIDHVIEAVEFDASE
                     PWYRKCLYKIGFIAAQSRLLQRPVLWVDVDSRLIRNPWPLFSEDMDFAGFRRDFRPLN
                     APLLHLPRFWHPGIVYYGCSKNAQAFLAQMESLTAAAPENVTDDYILHEAWLQGGDSL
                     RSHVLVPSAVQMVPADEIQPDTVFLFGSSGNVRAEARNVVQHQPEIERNREQVLHLLI
                     GSAMKRKSYKEAYHLARAEMLAPSASPQSVVKFANVLSILGRRKAARSTLEKAAFDRD
                     APNAEALRRLCEMALNARDVEGYAELERKGGEGAHAESQLTLRSFKLLFQLEEAANQL
                     RIRPAERPVFYWMRSPYPGNFGDLLTPYLALKLFQVPVRWAPARDAAALGVGSIARLA
                     RKDSVVWGSGIARASDSIEPRARWCAVRGPQTAQCLSKAGGQVPAAMGDPALLLPRVR
                     KKAASAGGIALIPHVSQISWIEENLKIASPEGAEVKLLSPLCDSLEAIDAFIDAISRA
                     DLVLSSSLHGLIVAHAYGIPAAWIQLKNGAPAVTGDGVKFADHLESVGLPPALYS"
BASE COUNT          754 a         1058 c         1182 g          888 t
ORIGIN      
        1 atcttcccgg tgaatatctg gaaatgctgt ttgaaatagc cgtggaggaa gccgagcggc
       61 tttcgtgggt gttattggcg gattttggaa aaatcctgcc ggagagctgg ttgaaagtgg
      121 cccaggcggc gggggcagcc aaaagggatc agttgacggc ggagattttg aaaaggctca
      181 gcgaaccgga gattctggag aaattctccg aggagcaagc aaaggaagtg aaatggatgg
      241 gtgacatgat cgctcacgga aagcgatcgg aggcgcctga tccggacaag atttctacca
      301 tttccctggg ggtcattgat tatcgctcga tcgattatgt ccgcgcctcc aaaaacattg
      361 gcgattttgt gcagacggtg gcctgccttt ccaatctggc ccgtttccgg aatacgcgct
      421 tttccgggga cgaggggctc gcccaatttg tcactggtct caaggaccgt gtttatccgg
      481 accgtcacat cgaagggccg ccggtggaca tcaaccttgt ggcaaccaac cgggactaca
      541 ccagccggtc gggggctccg gcagggactt ggctgatcgc cttcggctgg cacatgcaca
      601 gcctttttaa tgcggcttac gattttccgt tcgacgactc cctgaggccg atcttctttt
      661 cctttcacat taataatccg gacctgttga cggacgaggt cattgcctat ttaaaaagca
      721 aggccccgat tggttgccgg gactggaata cggtggacat gttgttcagc cacggggtgg
      781 acgcgttttt cagcggctgt ctgaccacca ccgttgacaa tattttgcgg ggcagccgca
      841 agagtccgtc cgagagcgcc tggaaggtgg cattcgtcaa cgttccaccg gctcctgagg
      901 agaaggccac tccctacgat accatcaaca tcatgaagcc cgagatcttg gagagaacct
      961 tctcggaaaa ccttgccgcc gcggtggagc aattggagga atacgcccga tacgaccttt
     1021 tggtttcctc caagctgcat tgttacctgc cctccacggc catggggctt aaggtggatt
     1081 tccgccccaa acggccggcg gacattcgtt ttgaaggtct ctatggcctg gatccgaaga
     1141 ccatcgaaac cgttcgccag cccattctcc gtaaacttca gcttattttt gaaaagattt
     1201 ttacgggggc ggccgaggaa gaagtgtatt cctattggcg tgaggtctgc gcgccggatg
     1261 tggaaagggc ccgtgcctat tgccagccgc cgccgccggc ccctccttcg tttgatatcc
     1321 aggttgcggc cgatcgggtg ctttccgcct ggccgtcgga tcaggctggc gaagcaaacg
     1381 gcgattatat cgaagtcgct ttctcgaccg atgagaatct gaaggatcaa ctgctggtgg
     1441 tattggaatc cgcggtcgct cacacgtcgc gcccgatccg ggcctggatt ctttcccgcg
     1501 gtttggggcc ggatgatttt gcccgttacg aaaagctttt cccgcaaatc cgtttccgtt
     1561 ttctgccctg cgactcggtc gattacggcc aaatcctggg gctgctcatc cacacgacgg
     1621 tggcgacgat ggaccggctg ttgctgccgg tgctggtgcc ggcgaaaaag atgatctatc
     1681 tggatatcga cctgctggtc cgggccgact tggcggagct tttcgatatc ccaatggggg
     1741 agagcccgct ggccgggagg acgagcatga tggaaagctg gtccacggga taccacttgg
     1801 tatatcgttc ggctaatcgc ctggatgccg cccgctcgat cgagctccgg cgccggatgc
     1861 acgccctgca tgacctgcgg ttcccgggct ttaacgccgg cgttttgttg ttgaatctgg
     1921 atcaaatgcg caaggatcgc ttttgcgagg agtatcttcc cttcgccgag cgatacggca
     1981 tgaatgacca ggaagtcttg aattgttatg ccggtgaccg ccgcatcgag ctggacctga
     2041 actggaactg tctggcggcc caggatatga tcgatcacgc caagatcatt cattgggccg
     2101 gccacgtgaa agcctggaat tgcaactggg tcgttggcaa aaactactgg gactaccacg
     2161 aagccaaggt tcgtgcgcgg ctggcccagg agtcgtgaat ggcattctga tcagttttta
     2221 ttcccgcgat ccctattatc agggttgtgc cgaaaaactt cgggaggatt gcatccggct
     2281 gaaaatcgac cacgtgatcg aggccgttga attcgatgct tccgagccgt ggtatcgaaa
     2341 gtgcctttat aagatcggct tcattgccgc ccagtcgcgc ttgctgcaaa gacccgttct
     2401 ttgggtggat gtggactccc gcctcatccg gaatccctgg ccgctctttt ccgaggacat
     2461 ggattttgcc ggattccggc gggattttcg gccgctcaat gccccattgc tgcatctgcc
     2521 ccggttctgg catccgggga ttgtctatta cggttgctcg aagaatgccc aggcctttct
     2581 ggcgcagatg gagtccctga cagcggcggc gccggagaac gtgaccgatg attacatttt
     2641 gcacgaagcc tggctccagg gcggtgattc cctgaggtcg catgtcttgg ttccgtcggc
     2701 ggtgcagatg gtgccggcag acgagatcca gccggacacc gtgttcctct ttggatcgag
     2761 cggcaatgtg cgggcggagg cgcgcaatgt cgtccaacat caaccggaga tcgagcgcaa
     2821 tcgggaacaa gtgctgcatc ttttgatcgg aagcgcgatg aagcgcaagt cttataaaga
     2881 ggcttatcat cttgcgaggg cggaaatgct ggctccgtcc gcttcgcccc aatccgtggt
     2941 caaatttgcc aacgtgctct ccattctggg gcgccgcaag gccgcgagga gcaccctgga
     3001 aaaagccgct tttgaccggg acgcgccgaa tgcggaagcc ctgcgccggc tttgcgagat
     3061 ggctttgaac gcccgggatg tcgaaggtta tgccgagttg gaacggaaag gcggggaagg
     3121 cgcgcatgcg gaaagccagc tgaccctccg aagctttaag ttgctctttc agttggaaga
     3181 ggcggcgaac cagcttcgaa tccgcccggc ggaacgcccg gttttctact ggatgcgttc
     3241 tccttacccg ggaaacttcg gcgacctgct gaccccctac cttgcgctca aactttttca
     3301 ggtgcccgtg cgttgggcgc ccgcccggga tgcggcggcc ctgggggtgg gaagcattgc
     3361 ccgcttggcc agaaaggatt ccgtcgtgtg ggggagtggc atcgcgcgtg ccagcgactc
     3421 cattgagccg agagccagat ggtgcgcggt tcggggaccc cagacggctc agtgtttatc
     3481 gaaggctggc ggccaggtgc ccgccgcaat gggagatccg gcgcttttgc tgccccgggt
     3541 tcggaaaaaa gccgcttcgg cgggcggaat cgccctcatt ccccacgttt cgcagataag
     3601 ctggatcgaa gagaacctga aaattgcttc accggaggga gcggaagtta agcttctttc
     3661 accgttgtgc gattcgctgg aggcaatcga tgcatttatt gatgcgattt cccgggcgga
     3721 cctcgtgcta tcgagctctt tgcatggttt gattgtcgcc catgcctacg ggatccccgc
     3781 tgcgtggatt caattgaaga atggcgctcc cgccgtgacg ggagatggcg tcaaattcgc
     3841 cgatcatctg gagtcggttg gattgcctcc ggccttgtat tc
//
LOCUS       DATDAY010000304         2975 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_1163200, whole genome shotgun sequence.
ACCESSION   DATDAY010000304 DATDAY010000000
VERSION     DATDAY010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2975)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2975)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2975
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1163200"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..752)
                     /locus_tag="VIS74_06090"
     CDS             complement(<1..752)
                     /locus_tag="VIS74_06090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792850.1"
                     /translation="MPQDTNQLPAEHVRLLEDARREQFWKLWGPYLSERQWGTVREDY
                     SADGDVWVALTHDEARSRAYRWGEDGLLGWTDRFGRLCFSLALWNGHDAILKERLFGL
                     TGPQGNHGEDVKELYYYLDSTPTHSYAKGLYKYPQAAFPYERLVEENARRSREQTEYE
                     LLDTGVFDGNAYFDVFVEYAKASIQDTLIRIHIWNRGSAGAPLHVLPTLTFRNTWNWG
                     AIKEESLQRPLIAESAPGRLSLQHETMGRYVFA"
     gene            complement(844..1614)
                     /locus_tag="VIS74_06095"
     CDS             complement(844..1614)
                     /locus_tag="VIS74_06095"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HEY5792851.1"
                     /translation="MGKLDGKVALVTGSGQGIGQAIAIRLAQEGADVVVDDRKIGESA
                     EETAAEIRKLGRRTYIFQGDLSNLDDNRRMIAESVAALGKLDILVNNAGVEKRADFWD
                     VTEADYDFVLNINLKGTFFATQDFVKHLLETKRPGRVINISSVHEELVFPHFSTYCAS
                     KGGLKMLCRDLAVELGPYGITVNNIAPGAIETPINTKLLNDPALLKALMSQIPLGRLG
                     QPSDVSGLAAFLASDDAAYVTGSTFVVDGGLLRNYHEQ"
     gene            complement(1631..>2975)
                     /locus_tag="VIS74_06100"
     CDS             complement(1631..>2975)
                     /locus_tag="VIS74_06100"
                     /inference="COORDINATES: protein motif:HMM:NF016513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEY5792852.1"
                     /translation="MKRAVAMTDPAEWPLKEDLQPVFERFAVHLRALKADGRLPSLPS
                     EEILPFSAHYLGLRELAELLGIYREELASLRQPAEPTLEAARQIPGTFIENFWIHNGI
                     KGGLVTVLALVLVDWLKPPGGTMIPLAAWIFTILSRVFLRWQGDRRSFQYAFITAILG
                     IPYLALLLVLAPVLSSYAAANALIFACFFVFGYLCIQLKGITFWMQVGMFGVISAIGL
                     NPQSPVGFPQIVDAYFGIVIAMFLSAIVQRFLWPVTPPIELRHSLDRFLEACRNLLRH
                     PADPRIHLWRKQIALASSQAAAWAEQFTTPEFPEGEREKWQAALATLRATADQLRVCM
                     KDAFLPEFGPLIALMRFKPIQYRERHDAILAEFQEVINGRRPAETLAIAPEMPLVDEF
                     KRLRDARAFADFDMDLVFHFYGHIARCQRLHLETQDCIDRLKSLKLERYMGDFAL"
BASE COUNT          563 a          892 c          974 g          546 t
ORIGIN      
        1 gcaaacacat accggcccat ggtctcgtgc tggagcgaga ggcggccggg ggcggattcg
       61 gcgatgagcg ggcgctggag cgactcttcc ttgatcgcgc cccagttcca cgtattgcga
      121 aaagtcagcg tgggcaggac gtgcagcggc gcgccagccg agccgcggtt ccagatgtgg
      181 atgcggatca gcgtgtcctg aatcgaggcc ttggcgtatt ccacgaacac gtcgaaatac
      241 gcgttgccat cgaacacccc cgtgtcgagc aattcatatt cggtctgctc gcgcgagcgg
      301 cgggcatttt cctcgacgag ccgttcgtag ggaaacgcgg cctgcgggta tttgtagagc
      361 cccttcgcgt aggaatgcgt gggcgtggaa tcgaggtaat aatacagctc tttcacgtct
      421 tcgccgtgat tgccctgcgg tccggtcagc ccgaagaggc gttccttaag gatcgcgtca
      481 tgcccgttcc acagcgcgag cgaaaaacac aggcggccga agcggtcggt ccagccgagc
      541 aggccatcct cgccccagcg atacgcccgg ctgcgcgcct catcgtgcgt gagggccacc
      601 cagacgtcgc cgtcggcgga ataatcctcg cgcaccgtgc cccattgccg ctcggacaaa
      661 tagggccccc agagtttcca gaattgttcg cgccgcgcgt cctcgagcag gcggacgtgc
      721 tccgccggaa gctggttggt atcctggggc atagggaaag atttaccgcg gaggcgctga
      781 ggcgcggagc cgaaaaatca tgatttcatt cccggcgcct ccgcgtctcg gcggtggatt
      841 ggattactgt tcgtgatagt ttcgcagcag tccgccgtcc accacgaagg tgctgcccgt
      901 cacgtaggcc gcgtcgtcgg aggcgaggaa ggcggcgagg ccggacacgt cggagggctg
      961 gcccaggcgg ccgagcggga tctggctcat cagcgccttg agcagcgcgg gatcgttgag
     1021 cagcttggtg ttgatgggcg tttcgatcgc gcccggcgcg atgttgttca ccgtgatgcc
     1081 gtagggcccg agttccacgg cgagatcgcg gcagagcatc ttgagcccgc ccttgctcgc
     1141 gcaataggtc gagaaatggg ggaagaccaa ctcctcgtgc acggagctga tgttgatgac
     1201 ccggccgggc cgcttggtct cgagcagatg ctttacaaaa tcctgggtgg cgaagaacgt
     1261 gcccttcagg ttgatgttga gcacaaagtc gtagtcggcc tcggtgacgt cccagaaatc
     1321 cgcgcgtttt tcgacgccgg cgttgttcac gaggatgtcg agcttgccca gggcggcgac
     1381 gctttcggcg atcattcggc ggttgtcatc caggttggaa agatcgccct gaaaaatata
     1441 ggtccggcgg ccgagcttcc ggatttccgc ggcggtctcc tccgcgcttt cgccgatttt
     1501 ccggtcgtcc accacgacat ccgcgccttc ctgggcaagg cgaatggcga ttgcctgtcc
     1561 gattccctga ccgctgccgg tcaccaacgc tacttttccg tcgagttttc ccataagctt
     1621 gtgcgagatt ttacagcgca aaatcgccca tgtaacgctc caacttcaag gattttaacc
     1681 gatcgatgca gtcctgcgtt tcgaggtgca gccgctggca gcgggcgata tggccgtaga
     1741 aatgaaaaac gagatccatg tcgaaatcgg caaacgccct cgcatcgcgc agccgcttga
     1801 attcgtccac cagcggcatt tccggggcga tggcgagcgt ttcggcgggc cggcggccat
     1861 tgatgacttc ctggaattcc gcgaggatcg cgtcgtgccg ttcgcggtat tggatcggct
     1921 tgaaacgcat caacgcgatc agcggcccga attcgggaag aaaggcgtcc ttcatgcaca
     1981 cgcgcagctg gtcggcggtg gcgcgcagcg tggccagggc cgcctgccat ttttcccgtt
     2041 cgccctccgg aaattccggg gtggtgaact gctccgccca cgccgccgcc tggctggagg
     2101 ccagcgcgat ctgcttgcgc cagaggtgaa tccgcggatc ggccgggtgg cgcagcaggt
     2161 tccggcaggc ctcgaggaag cggtcgaggc tatgacgaag ttcgatcggc ggcgtcaccg
     2221 gccacaaaaa ccgctggacg atcgcggata aaaacatggc gatcacgatg ccgaaatacg
     2281 cgtccacgat ttgcgggaat cccaccggcg actgcgggtt gaggccgatc gcggaaatca
     2341 cgccgaacat gccgacctgc atccagaacg tgattccctt cagctggatg cagaggtagc
     2401 cgaagacgaa gaagcaggcg aagatcaggg cattcgccgc cgcgtaactg ctgagcaccg
     2461 gcgcgagcac gagcagcagc gccaggtagg gaatgccgag gatcgccgtg atgaacgcat
     2521 actgaaagct gcgccggtcg ccctgccagc gcaggaacac acgggagaga atcgtgaaaa
     2581 tccacgcggc gaggggaatc atggtgccgc cgggcggctt cagccaatcg acgagcacca
     2641 gggccagcac ggtgacgagg ccgcccttga tgccgttatg aatccagaag ttttcaatga
     2701 acgtgccggg aatctggcgg gcggcttcca gggtaggttc ggccggctgg cgcagactcg
     2761 ccagttcctc ccggtatatg ccgagcagtt cggccagctc gcgcaacccc aggtaatgcg
     2821 cggagaaggg cagaatctcc tcgctcggca gggacggcag gcggccgtcc gccttgaggg
     2881 cgcgaaggtg gaccgcgaaa cgctcgaaca ccggctggag atcttctttc agcggccact
     2941 ccgccggatc ggtcatggcg acggcgcgtt tcatt
//
LOCUS       DATDAY010000305         2880 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_846288, whole genome shotgun sequence.
ACCESSION   DATDAY010000305 DATDAY010000000
VERSION     DATDAY010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2880)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2880)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2880
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_846288"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..280)
                     /locus_tag="VIS74_06105"
     CDS             complement(<1..280)
                     /locus_tag="VIS74_06105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEY5792853.1"
                     /translation="MAAVQANFRVVAETPDLLVVDKPPGLLVHPTKPGGPKTLLDALR
                     ELLAYEIENGGQVSIINRLDRETSGLVLIAKNAEAASRCARAMQAGRIH"
     gene            315..830
                     /locus_tag="VIS74_06110"
     CDS             315..830
                     /locus_tag="VIS74_06110"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEY5792854.1"
                     /translation="MEKSTDSPSSESLEALGKFKLFAGFKAAELATLVSLAEEKNYAA
                     GELIIREGGLGESMFVILAGEVVVFREAQGKRVEFTRMRVGDSFGELALVDDGPRSAS
                     VAAVVPTRVLRITQDVVAVLAGVRPAAAIHLLRAIGKSLVARLRTGNQRYLDIVLTGS
                     IPIASSGDESA"
     gene            856..2160
                     /locus_tag="VIS74_06115"
     CDS             856..2160
                     /locus_tag="VIS74_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase/3-hydroxyacyl-ACP
                     dehydratase"
                     /protein_id="HEY5792855.1"
                     /translation="MENQRTLQKSASLTGTSLHTGENVTLTLHPAPPNHGFKFRRKDL
                     PDEPVVEARVENVRTVERATTLVEGGTKIHTVEHVVSALSGLGVDNALIEMDANEPPI
                     GDGSAELYAQLIKKAGIVEQEAPRVYFQPREPIHVETKDGSILTIFPDDKFRISCTQV
                     GPEGRFTQYRSLEITPETYEAEICRARTFVFYEDVKPLMDKGLIKGGSLENAIVARGD
                     AVLSKEPLRFPDEFVRHKILDIVGDLALAGRRIKGHIVAVKPGHGPNTELARAVSKRH
                     AELVAMSAAPITSTAAVFDINEIMKTLPHRYPFLMVDRIVDFTEDMKAVGVKSVTINE
                     PYFQGHFPGHPVMPGVLQLEAMAQVASIVMMRKTENAGKIGYFMSADEVKFRKPVMPG
                     DTLFIHAEMTKAKKTLGKAECKCMVNNEVVSEGKLLFGLIDQ"
     gene            2157..>2880
                     /gene="lpxA"
                     /locus_tag="VIS74_06120"
     CDS             2157..>2880
                     /gene="lpxA"
                     /locus_tag="VIS74_06120"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HEY5792856.1"
                     /translation="MTEIHPTAVVDPTAEIGEDSTIGPYCVIGAQVRLGRGNRLLNHV
                     TVAGPAEIGDGNVFYPYTSIGQRTQDLKYAGEPTHLKIGNANSFREFCTVNRGTAPGS
                     FTTVGSHGNFLAYAHIAHDCTVGDHVIFSNNGTLAGHCLVEDHAVIGGLSGVHQFCRI
                     GRHAIIGGCTKIVQDVPPFMIADGNPAEIRGINQVGLERRGFTSGHVRALREAYRIVW
                     RSNQNVSQSLESIEQKIELSEPV"
BASE COUNT          554 a          927 c          888 g          511 t
ORIGIN      
        1 tgtggatgcg gcccgcctgc atggcccggg cgcaccggct ggcggcttcc gcgtttttgg
       61 cgatgagcac gagcccgctg gtctcgcggt cgaggcggtt gatgatcgaa acctggccgc
      121 cgttctcgat ttcgtaggcc agcaattccc gcagggcgtc gagcagcgtt tttgggccgc
      181 ccggcttcgt cggatgcacg agcagccccg gcggcttgtc caccaccagc aggtcgggag
      241 tttccgccac cacgcggaag ttggcttgca ctgcggccat tttcggagat tctcccaatc
      301 acacccgcac tgcgatggaa aagtccactg attccccatc ttccgaatcc ctggaggcgc
      361 tcggcaagtt caagctgttc gcgggattca aggccgccga gctggccacg ctggtttccc
      421 tggcggagga aaaaaactac gcggccgggg aattgatcat tcgcgagggc gggcttggcg
      481 aaagcatgtt tgtgatcctc gccggggagg tggtggtttt ccgcgaggcc cagggcaaac
      541 gcgtggaatt cacccgcatg cgcgtgggcg attcctttgg cgagctggcg ctggtggacg
      601 acggcccccg ctccgccagt gtcgccgcgg tggtcccgac gcgggtgctg cggattaccc
      661 aggacgtggt ggccgtgctg gccggagtcc gcccggcggc ggccattcat ttgcttcgcg
      721 ccattggcaa atccctggtg gcccggcttc ggaccggaaa tcagcggtat ttggacatcg
      781 tgctgaccgg atcaattcct attgccagca gcggtgacga aagcgcatag tggcgggctt
      841 caaaacctgt ccgtagtgga aaaccagcgc acgttacaaa aatccgcctc gcttaccggc
      901 acctccttgc acaccggtga gaatgtgacc ctcacccttc atccggctcc gcccaaccat
      961 ggattcaagt tccgccgcaa ggacttgccg gatgagccgg tcgtcgaggc cagggtggaa
     1021 aatgtccgca ccgtggagcg ggcgaccacc ctcgtcgaag gcggcacgaa aattcatacc
     1081 gtcgaacacg tggtgagcgc gctttccggc cttggcgtgg ataatgccct catcgaaatg
     1141 gacgccaatg agccgccgat cggcgatggc agcgccgaac tttacgcgca gctcatcaaa
     1201 aaggccggca tcgtcgagca ggaggcgccg cgggtttatt tccaaccgcg cgagcccatt
     1261 cacgtggaga cgaaggatgg ctccatcctc accatctttc cggatgacaa attccgcatt
     1321 tcctgcaccc aggtcggccc cgaggggcgt ttcacccaat accgcagcct cgaaatcacg
     1381 ccggaaacct acgaggccga aatctgccgc gcgcgcacct ttgtgttcta cgaggacgtc
     1441 aagccgctca tggacaaggg cctcatcaag ggcggcagcc tggagaacgc aatcgtcgcc
     1501 cgcggggacg cggtgctgag caaggagccg ctgcgcttcc cggatgaatt cgtccggcac
     1561 aagatcctcg acatcgtcgg cgatctcgcc ctcgccggcc gccggatcaa gggccacatc
     1621 gtcgccgtca agcccggcca cggaccgaac accgaactcg cgcgcgccgt ctccaaacgc
     1681 cacgccgagc tggtcgccat gtccgcggcc cccattacct ccacggcggc ggtcttcgac
     1741 atcaatgaga tcatgaagac gctgccgcac cgctatccct tcctcatggt ggatcgcatt
     1801 gtggacttca cggaggacat gaaagccgtc ggcgtcaagt cggtgacgat caacgaaccc
     1861 tatttccagg gccacttccc cggccatccg gtcatgcccg gcgtgctgca gctcgaagcc
     1921 atggcgcagg tggcgagcat cgtgatgatg cgcaagaccg agaacgcggg aaaaatcggc
     1981 tacttcatga gcgccgacga ggtgaaattc cgcaagcccg tcatgccggg ggacacgctg
     2041 ttcatccacg cggagatgac caaggccaag aagacgctcg gcaaggcgga gtgcaagtgc
     2101 atggtcaaca acgaggtggt ttccgagggc aagcttctgt ttggtttgat tgatcaatga
     2161 cggaaatcca ccccacagcc gtggtggacc cgacggcgga gatcggcgag gattccacca
     2221 tcggtcccta ctgcgtgatc ggcgcgcagg tccgtttggg ccgcggcaac cgcctgctca
     2281 atcacgtgac ggtggccggc ccggccgaga tcggggacgg gaacgtgttt tacccctaca
     2341 cgtccatcgg ccagcggacg caggatttga aatacgccgg cgagccgacg catctgaaga
     2401 tcggcaatgc gaactcgttc cgcgaattct gcaccgtgaa ccgcggcacc gcgcccggtt
     2461 cgttcacaac tgtgggcagc cacggcaatt ttctcgccta cgcgcacatc gcgcacgatt
     2521 gcaccgtggg cgatcacgtc attttctcca acaacggcac gctcgccggc cattgcctcg
     2581 tcgaggatca cgccgtcatc ggcggcctga gcggcgtgca tcaattctgc cgcatcgggc
     2641 gccatgccat catcggcggc tgcaccaaga tcgtgcagga cgtgccgccg ttcatgatcg
     2701 ccgacggcaa cccggcggag attcgcggca tcaaccaggt cggcctcgag cggcggggct
     2761 tcaccagcgg gcatgtccgc gcgctgcgcg aggcataccg gatcgtctgg cggtcgaatc
     2821 aaaacgtctc gcagtcgctc gaaagcatcg aacaaaagat cgaactgagc gaaccggtcc
//
LOCUS       DATDAY010000306         3010 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_1517591, whole genome shotgun sequence.
ACCESSION   DATDAY010000306 DATDAY010000000
VERSION     DATDAY010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 3010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3010
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1517591"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            237..1127
                     /locus_tag="VIS74_06125"
     CDS             237..1127
                     /locus_tag="VIS74_06125"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HEY5792857.1"
                     /translation="MPLTLLILAAGMGSRYGGLKQLDPMGPNGEVVMDYSVYDAWRSG
                     YSEVVFVIRRDFAEDFKKFIGSRFENRIKVSYVFQDMTNLPEGFSVPADRTKPWGTGH
                     AILAAREVIQNPFVAINADDFYGQDAYQKIADFFAASPAPTAFAMPGFRLASTLSEHG
                     TVARGICETDAEGYLRQVEELTAIARTERGITNGGDPRLFTGEESCSMNFWGLTPAAF
                     SLLDAGFREFLTASIQNPKAEFYIPQAISEMIQRGTASVKVLPTTSKWFGVTYQADKP
                     EVVASLRRLIDSGEYPAKLW"
     gene            complement(1229..1939)
                     /locus_tag="VIS74_06130"
     CDS             complement(1229..1939)
                     /locus_tag="VIS74_06130"
                     /inference="COORDINATES: protein motif:HMM:NF014630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HEY5792858.1"
                     /translation="MLTEIEQLLALQDRDQRVLSLKKELTSLPAEQKARETQLAQAAA
                     RLDQSKIRAKQIEIDRKRLEGEVKAKEDTINRYKQQQLQTRKNEEYSALAHEIEAAGK
                     VIGALEDQELALMEESDLLKPQIAQAETAFQEDKKHIEAHLHGLGVKKINLEAQLAEF
                     EKLSEQTAAGVEEDLLDRYRRLFKTKEGRAVVALDPDHEVCAGCHMKVTTQTVVEVKA
                     EKSVVHCPQCGRILFLEP"
     gene            complement(2050..2754)
                     /locus_tag="VIS74_06135"
     CDS             complement(2050..2754)
                     /locus_tag="VIS74_06135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792859.1"
                     /translation="MKLLIFIGVILLGAAGWLGYDYVSKHPKLIESLIPPRDSQGRLL
                     VDCPHCQAAGTVKCGAPRCKNGRVNCPGPCLKLSTPGWKKHPLPGYDPDTLWHTFNVK
                     GGEQRVSQGHIGEVFEVKNGVFANLGKCKVCQGTTTVDCKICAGTGAVACPVCKGAKR
                     VSDTGNPPARPNVSPSAPVSPPPASLPPAAMKIIRFKNGRTLEGTIIIRDEKTVWIRT
                     RSGEKIQVPAADLILP"
     gene            complement(2751..>3010)
                     /locus_tag="VIS74_06140"
     CDS             complement(2751..>3010)
                     /locus_tag="VIS74_06140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792860.1"
                     /translation="FLIAFVAGRQLPAELQFIRPDGFLRHLRIGVLLLWEVLLAYGVI
                     FSLSAGDAGMIFTGTLAAALLLLLIAAPPKSTADKPAPLIS"
BASE COUNT          552 a          901 c          921 g          636 t
ORIGIN      
        1 ggaggagcct tgttcccccg ggctttgttt ttttggcatt gggaaaattt tcacgcggag
       61 acgcggagaa tcggtaaaaa tatgccggat tttctccgcg ccttcgcgtc cccgcgtgag
      121 tttttgcccc attcggctca cgaaaaagtt ggacagacgc gccgtccaac ggcatgacaa
      181 cgggcagcgc tcgcggcagg ggccgcggcg ctccccaaaa tcctccgatt cacttcatgc
      241 ctcttactct cctgattctc gcagccggaa tgggcagccg ttatggcggc ctcaaacaac
      301 tcgaccccat gggtcccaac ggcgaggtgg tcatggacta ttccgtctat gacgcctggc
      361 gcagcggtta ttccgaggtg gtttttgtca tccgccggga cttcgccgaa gacttcaaaa
      421 aattcatcgg cagccgtttc gagaaccgca tcaaggtctc ctacgtcttc caggacatga
      481 cgaatctgcc cgagggcttc tcggtcccgg cggatcggac caaaccctgg ggcaccggcc
      541 acgccattct ggccgcgcgc gaggtgattc agaatccgtt tgtggccatc aatgccgacg
      601 atttttacgg ccaggacgcc tatcaaaaaa tcgccgattt cttcgcggcc tccccggcgc
      661 cgaccgcctt tgccatgccg gggttccggc tggccagcac gctttccgaa cacggcaccg
      721 tggcccgcgg catctgtgag accgacgcgg aggggtatct ccgccaggtc gaggagctca
      781 ccgccattgc ccgcaccgag cggggaatca ccaacggcgg cgacccgcgc ctcttcaccg
      841 gcgaagaatc ctgctcgatg aatttctggg gcctgacccc ggcggcattt tcgctgctgg
      901 acgccggctt ccgggaattt ctgacggcgt ccattcagaa cccgaaggcg gaattttaca
      961 tcccccaggc catttccgaa atgatccagc gcgggaccgc gagcgtgaag gtgctgccga
     1021 cgaccagcaa atggtttggc gtgacctatc aggccgacaa gccggaagtg gtggcgtcgc
     1081 tgcggcggct gatcgactcg ggcgaatacc ccgcgaagct ctggtagaaa cgaaaccgga
     1141 ttgccggatt tgccggatta actggaaaat aaaaatccgg ttaatctcgc caatcccgtt
     1201 tggtttgaaa tgagcgcccc ggcgcgcttc agggctccag gaagagaatc cgtccgcatt
     1261 gcgggcaatg gacgacggat ttctccgcct tgacctcgac gaccgtttgc gtggtcacct
     1321 tcatgtggca gcccgcgcag acttcgtgat cgggatccag ggcaaccacc gcccggcctt
     1381 ccttggtttt gaataaccgg cggtagcggt cgagcagatc ctcctcgacg ccggccgccg
     1441 tctgttcgct gagtttttca aattccgcca gctgcgcttc gagattgatc ttcttgacgc
     1501 ccaacccatg gaggtgggct tcgatgtgtt tcttgtcctc ctgaaaggcc gtttccgcct
     1561 gcgcgatctg gggcttgagc aagtccgatt cttccatcaa tgccagctct tgatcctcca
     1621 gggcaccgat gacttttccg gcggcttcga tctcgtgcgc cagcgcggaa tattcctcat
     1681 tcttccgcgt ctgcagctgc tgctgcttgt agcggttgat cgtatcctcc ttggccttga
     1741 cctcgccttc gagccgcttg cggtcgatct cgatctgctt ggcgcgaatt ttggactgat
     1801 ccaatcgcgc cgcggcttgg gcgagctggg tttcccgggc tttttgttcg gccggcagcg
     1861 aggtgagttc ttttttcagc gagagcacgc gttgatcccg atcctgcaaa gcgagcaatt
     1921 gttcgatctc cgtcaacatc ggaccgagag ttaagggatt cccccggcgc ttggcaattc
     1981 cgcaggcggc tggaaggcgc ttttttgccg gaggggggaa aagcgggaag gttctcccgc
     2041 tctgccgctt cagggcagga tgagatcagc cgccggaacc tggatttttt ctccgctccg
     2101 cgtgcgaatc cataccgttt tctcatcgcg aataatgatg gttccctcca aggtgcggcc
     2161 gttcttgaag cggatgattt tcatcgccgc cgggggcaaa ctcgccggcg gcggcgaaac
     2221 cggagcggag ggcgagacgt tggggcgggc ggggggattg ccggtgtcgg agacacgctt
     2281 ggctcccttg caaaccggac aggcgaccgc gccggtcccc gcgcagattt tgcagtccac
     2341 cgtggtggtt ccctggcaaa ccttgcactt gcccagattg gcgaatacgc cgttttttac
     2401 ctcgaagact tcgccgatgt gcccctggct caccctctgc tcgccgcctt tgacattgaa
     2461 agtatgccaa agtgtgtcgg ggtcataccc gggaagggga tgttttttcc agccgggagt
     2521 ggagagcttc aggcacgggc ccggacagtt cacccggccg tttttgcagc ggggagcacc
     2581 gcatttgacg gttccggccg cctggcagtg cgggcaatcc accaataatc ggccctggct
     2641 gtcgcgcggc gggatgaggg attcgatcag cttgggatgc ttggaaacgt agtcgtagcc
     2701 cagccatccg gcggctccga ggaggattac acctataaag atcagcagct tcatgaaatc
     2761 aggggggcgg gtttgtcggc ggtggatttg ggcggggcgg cgatcaataa aagcaggagg
     2821 gcggcggcca gggtgccggt aaaaatcatg cccgcgtcgc cggcggaaag agagaagatc
     2881 accccgtaag ccagcagcac ctcccagagc agcaacacgc cgatgcgcag gtggcggaga
     2941 aagccgtcgg gccggatgaa ttgaagctcc gcgggcaatt gccgcccggc cacgaaggcg
     3001 atgaggaatg
//
LOCUS       DATDAY010000307         2728 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_1731125, whole genome shotgun sequence.
ACCESSION   DATDAY010000307 DATDAY010000000
VERSION     DATDAY010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2728)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2728)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2728
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1731125"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            141..518
                     /locus_tag="VIS74_06145"
     CDS             141..518
                     /locus_tag="VIS74_06145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792861.1"
                     /translation="MRSFLALILWFSGAIAGLAQELPVLLATELDQLREKAGQEVVVE
                     GDVVSVGKTANGSLTFINVGLPKKMGFVALVRQSSYRAFPEGFDGFKGQKVRLRGVIT
                     IYRENQPQIEVQSPDQISIVNPS"
     gene            complement(534..1130)
                     /locus_tag="VIS74_06150"
     CDS             complement(534..1130)
                     /locus_tag="VIS74_06150"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006983224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HEY5792862.1"
                     /translation="MFTGLVEATTAVIGIAPRAEGAALRLRPAPALGQLAPGDSLAVN
                     GCCLTVTRQTAGELKFDVLLETLRRTNLGALEPGSVVNLERSLRADSRLGGHFVQGHV
                     DTTAALRERREANGDLYLRIELPPESAGYVAFKGSISLNGVSLTVAEVLPDSFAVWII
                     PHTRQHTNLDALRPGGRINVEFDILAKYVERLTSVRAA"
     gene            complement(1137..2429)
                     /locus_tag="VIS74_06155"
     CDS             complement(1137..2429)
                     /locus_tag="VIS74_06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006983555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HEY5792863.1"
                     /translation="MTPLLAYASDPSPWMILPFGILLLSIALMPFIHAHWWERHYPKV
                     AVALGAITTGYYLFGLKNAERMLHVAHEYVSFIALIGSLFVVAGGIHITVKGEARPWI
                     NALFLFIGAVLANFIGTTGASMLLIRPWIRMNKYRITTFHIVFFIFIVSNVGGCLTPI
                     GDPPLFLGYLKGVPFLWVLEHCWIAWLICTALLIGMFFIFDTLNFRRPPKELTEKFTA
                     SETWRFQGLNNAFLLAVIIGAVFLPSPWREILMLGAAAASYFGTSREIHQANRFNFAP
                     IQEVAWLFAGIFATMVPALDYLERHASSLGLHSPMQFYWFTGTLSAFLDNAPTYLTFL
                     AAALGDQGLSISKPADIHTFIETDNHFLVAISLGAVFFGAVSYIGNGPNFMVRNIASQ
                     LGVKMPSFFGYIFKYSAPILIPIFALIALLFFSPWRIF"
BASE COUNT          598 a          759 c          886 g          485 t
ORIGIN      
        1 ggcaaggacg ataacgaggg cgagacgaaa gttcatgggc gatcctggga atgcttacct
       61 atatgtcacg caaccggagg tggcaatttt tttgagccgg gacttggcgg gaaacggcgg
      121 gcgggttatt tcgagggagg atgagatctt tccttgcgct gattttatgg ttttcgggag
      181 cgattgcggg cctggcccag gaattgccgg tcctgctggc cacggagctg gatcaactcc
      241 gggaaaaagc gggacaggaa gtcgtcgtgg aaggcgatgt cgtcagcgtg ggaaagacgg
      301 cgaacggctc cctgaccttc atcaacgtcg gccttcccaa aaaaatgggc ttcgtggcgc
      361 tggtccggca gtccagttac cgcgcctttc cggagggatt tgacggcttc aaggggcaga
      421 aggtccggct caggggagtg atcacgattt accgtgaaaa ccagccccag atcgaagtgc
      481 aaagcccgga tcagataagc atcgtcaacc cgtcctgacc gtccgccggg gcttcaggcg
      541 gcccgcacgg acgtgaggcg ctccacgtat ttggcgagga tgtcgaactc cacgttgatc
      601 cggccgcccg ggcggagggc gtcgagattc gtatgctggc gcgtgtgggg aatgatccag
      661 accgcgaagg aatccggcag gacttccgcc accgtgaggc tgacgccgtt cagggagatc
      721 gagcccttga aggcgacgta gcccgccgat tccggtggaa gttcgatgcg caaatataga
      781 tcgccattcg cttcgcggcg ttcccgcagg gcggcggtcg tgtccacgtg gccctggaca
      841 aaatggccgc ccagccgcga atcggcccgg agggagcgct ccagattcac cacgctgcct
      901 ggctcgagcg cgccaaggtt ggtccgccgg agggtttcga gcagcacgtc gaacttgagt
      961 tcgcccgcgg tctggcgcgt gacggtgagg cagcagccat tcaccgcgag gctgtcgccc
     1021 ggcgcgagct ggccgagggc gggagccggg cggagccgca acgcggcgcc ttccgcgcgc
     1081 ggcgcgatgc cgatgacggc ggtcgtggct tcaactaaac cggtgaacat ggtgcgtcag
     1141 aatatccgcc agggtgagaa aaataaaagg gcgatgaggg cgaaaatcgg aatgaggatc
     1201 ggcgccgaat atttgaaaat gtaaccaaag aagctcggca ttttcacgcc cagctggctg
     1261 gcaatgtttc gcaccatgaa gttcgggccg ttcccgatgt aactgaccgc gccgaagaaa
     1321 accgcgccga gcgagatggc cacgaggaaa tggttgtccg tttcgatgaa ggtgtggatg
     1381 tccgcgggtt tgctgatgct caggccctgg tcgcccagcg cggcggcgag gaaggtcagg
     1441 taggtggggg cattgtccag gaacgccgag agcgtgccgg tgaaccagta aaactgcatc
     1501 ggcgaatgca gccccaggct gctcgcgtgc cgctccagat aatcaagcgc gggcaccatc
     1561 gtggcaaaga tgccggcaaa cagccacgcg acttcctgaa tgggcgcaaa gttgaagcga
     1621 ttcgcctgat gaatttcccg cgacgttccg aaatacgaag ccgccgcggc ccccagcatg
     1681 agaatttccc gccagggaga gggcaggaag acggcgccga tgatcacggc gagcaggaag
     1741 gcattgttca atccctgaaa ccgccaggtt tccgaagcgg tgaatttttc cgtcagctcc
     1801 ttcggcggac ggcggaagtt gagtgtgtca aaaataaaga acatcccgat cagcagcgcc
     1861 gtgcagatca gccaggcgat ccagcaatgc tcgagcaccc agagaaacgg cactcccttg
     1921 agataaccga gaaacagcgg aggatcgccg atcggcgtca ggcagccgcc gacattgctg
     1981 acgatgaaaa tgaagaaaac gatgtggaag gtggtgatgc gatatttgtt catccggatc
     2041 cagggccgga tcagcagcat ggaagccccc gtggtgccga tgaaattcgc gagcacggcg
     2101 ccgatgaaaa gaaaaagcgc gttgatccac gggcgcgcct cgcctttcac cgtaatgtga
     2161 atgccccccg ccacgacgaa aagcgaaccg atcaatgcga tgaagctgac gtattcatgc
     2221 gcgacgtgca gcatccgttc cgcgtttttc agcccgaaga ggtaatagcc cgtcgtgatc
     2281 gcgccgagcg ccacggcgac cttcggataa tgacgctccc accagtgcgc gtggatgaac
     2341 ggcatcagcg cgatgctcag gagcaggatt ccaaacggca gaatcatcca gggcgacgga
     2401 tcggaagcgt aggcaagcag cggcgtcatg atgagagttt cggcccgcta aaatcaaact
     2461 ccctgattcc ggcggcaagc agaatcggtc cgctccggat ttgatcgatg gttttccccg
     2521 agttttttta tgcatgcatc gcttcaagcg taggggcgct tgtgcggcaa gcgcctacaa
     2581 cccgccgggt atttcggaag cttcttttaa aaatcgctct aataatccga agcagcgggt
     2641 tccgccgcgg tgacgattgc cacgctggcc gaggcgccga ggcgattggc tccggcttcg
     2701 atcatcgccc gcgccgtggc gtaatccc
//
LOCUS       DATDAY010000308         3257 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_2144295, whole genome shotgun sequence.
ACCESSION   DATDAY010000308 DATDAY010000000
VERSION     DATDAY010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 3257)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3257)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3257
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2144295"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            825..2039
                     /locus_tag="VIS74_06160"
     CDS             825..2039
                     /locus_tag="VIS74_06160"
                     /inference="COORDINATES: protein motif:HMM:TIGR02249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integron integrase"
                     /protein_id="HEY5792864.1"
                     /translation="MRGFITKIAVEGKELWQVRQAAEALEVFFTGVEPQAWAKHWPED
                     LIPGAGKEYLRIREPADLGRRPAEGPLAEEFAGRADTGPLPERYAGFVNEVREVLRAE
                     RYSYRTEQSYLDWARRFLIFAQPKSRAAIHWPQAGAYLDYLTLKRRVSASTLNQALSA
                     LQFVFAQVLKRKAGGMEGLKRPARSQRVPTVLTREEVAALFAQMEGTGRLMAELMYGS
                     GLRVMECARLRVKDVDFGGQHIVVRGGKGDKDRRAPLPRKVEPALRAQIESARKRWEK
                     DRALGVEGVFLPGALEVKYANASTEWSWFWIFPAEGLSEDPWTGKIRRHHLGVNGVQQ
                     LVKRAALRAGLVKPVSPHTLRHSFATHLLEGGADIRTVQELLGHADVSTTMIYTHVLN
                     KPGIAVRSPLDG"
     gene            complement(2360..3088)
                     /locus_tag="VIS74_06165"
     CDS             complement(2360..3088)
                     /locus_tag="VIS74_06165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY5792865.1"
                     /translation="MENPYEDPPVLAAYVLFHYGTARDALGSLPGPENAAEFPLRCVR
                     ELLNFDRLPRSARALDLGCAVGGSTFELSRFCQEVIGVDFSAAFIRAAESLRLESRFE
                     AELLEQGDQTWPFQARLPAGVHPERVRFQRGDALQLPETWTGFDVVLAANLLCRLSEP
                     RRLLERFSRLIPSGGQLLLTTPFTWLESFTPRARWIQGAGALKEILSPDFTLEHETEL
                     PFLIREHARKFQYGIAWGTCWRRR"
BASE COUNT          675 a          803 c         1149 g          630 t
ORIGIN      
        1 agcttcttct ggaaaacgta ggtcaggcgg caggtaatcc aagcgccaat aattgctccg
       61 aggactgctc ctcctccacc ggcgaaaagc gcgttaaccg tctcttggct cataatattt
      121 ctgcgaacgt cccagctgag ctatggccca caacggcctc acctcaacca aaggatcaat
      181 catgaaccgg aagtcggaac aaaaccgccg ggtggatggg gccattagct ccagcgcctt
      241 gttcgcattt ttgctttatg gaacaatccg aaatcatcgt taagcgcgag cggtggagaa
      301 caccaaatgc cgccattctc tcttgtctat actcttccct tgtaggcatt cgagcagtgg
      361 caaaggccct cgcaaggtcc aatccgtatc tgtgtggtag gcgatcacaa agtctcctgg
      421 aatgtatcgc gagtaaaaca aggcatcttg cgacttggct ttatcgcctg gggaatctgc
      481 gtgcggaagc gatatgcaga cgtgctcctt gccttctgca tcctcgaaat gaaaacaact
      541 tacacgaacc gcgtgaaaca tattttattg cgaacgtaat gtagaatgat gtatggattt
      601 agtggaaaaa ggtgatatca tcctaaatcg tgaacgaaga agatgaaaaa tctttcgtaa
      661 gatacaagcc ctcagggaat tgggagtcgc gttgggtgcg ttttcgcatg gcgctcatgc
      721 aacgaggagt tgaacccagg agccatgatt tttgccgggg atgggttttg gcgtggttaa
      781 atttcatcaa gccgcggaag tttgaccagg gcaatatgga tgacgtgcgg ggttttatca
      841 cgaaaattgc ggtggagggc aaggagcttt ggcaggtgcg gcaggcggcg gaggcgctgg
      901 aggttttctt cacgggggtg gaaccacagg catgggcaaa gcattggccg gaggatttaa
      961 tccccggagc gggaaaggag tatttgcgca tccgggagcc ggcggatttg ggccgcaggc
     1021 cggcggaggg accgttggcg gaggagtttg ccgggcgggc agacaccggg ccgctgccgg
     1081 agcgctacgc gggttttgtg aatgaggtga gggaggtgct gcgggcggag cggtattcgt
     1141 atcgcacgga acagtcgtat ctggattggg cgcggcggtt cttgatcttt gcgcagccga
     1201 agagccgggc ggcgattcat tggccgcagg cgggggcgta tctggattat ctcacgctaa
     1261 aacgccgggt gtcggcgtcc acgcttaatc aagcgttaag cgcgctgcaa tttgtgtttg
     1321 cccaggtgct caagaggaag gctgggggga tggaagggct gaagcgtcca gcaagaagcc
     1381 agcgggtgcc gacagtgctg acgcgcgagg aggtggcggc tttgttcgcg cagatggagg
     1441 gcacggggcg cctaatggcg gagttgatgt atggctccgg gctgcgggtg atggagtgcg
     1501 cccggctgcg ggtgaaagat gtggacttcg ggggacaaca tattgtggtg cgtggcggca
     1561 agggcgacaa ggaccggcgg gctccgctgc ctcgcaaggt tgagccggcc ctgagggcgc
     1621 aaatcgaaag cgctcggaag cgctgggaaa aggatcgcgc gctcggtgtg gagggggtgt
     1681 tcctgccggg ggcgctggag gtgaaatatg ccaacgcctc gacggaatgg agctggttct
     1741 ggattttccc cgccgagggg ctttcggagg atccgtggac aggcaagatc cggcggcacc
     1801 acttgggggt gaacggcgtg cagcaattgg tgaaacgcgc ggctctgcgg gcggggctgg
     1861 tcaagccggt aagtccgcac acgctgcggc attcgtttgc cacccatctg ttggaggggg
     1921 gagcggacat tcgcaccgtg caggagttgc tggggcatgc ggatgtgagc accacgatga
     1981 tttacacgca tgtgctcaac aagccgggaa tcgcggtgcg gtcgccgctg gatgggtgaa
     2041 ggaatgcgga atgcggaggt ccacgctttg atttttaatt cgcggttaaa atcgggatgg
     2101 atgcaaaaat tcagggtgag gggcgcggtg ggcagatgac tccttaaatg accgaatcag
     2161 gtcatgctgc cgcccttggt cgaaaggccc gttcttgaat gaaactccgg ggtttgcaac
     2221 tggagccgag ctttccaaaa ttcccgaaaa aaacaagggg atagtcacgg gggaaagcac
     2281 cttttttagg gcgcttcggc ggcaagggcc ggacgccctc cacgaggcag cggttgatgg
     2341 gaccaccggt gttttcgcct taacgccgcc gccagcaggt gccccaggcg atgccgtatt
     2401 ggaatttccg ggcgtgttcg cggatgagaa agggcagctc ggtctcgtgt tcgagggtga
     2461 aatcgggtga aaggatttcc ttcaacgctc cggctccctg aatccagcgg gctcggggcg
     2521 taaaggattc gagccaggtg aaaggcgtgg tcagaagcag ctggccgccg gagggaatga
     2581 ggcgggaaaa tcgttcgagc aaacgccggg gttcagaaag gcggcagagc aggttggcgg
     2641 ccagaaccac gtcgaacccc gtccatgttt cgggaagctg cagggcgtcg ccgcgctgga
     2701 agcggacgcg ctcgggatga accccggcgg gcaggcgggc ctggaacggc caggtttggt
     2761 ctccttgctc cagaagttcc gcctcgaacc ggctttcgag ccgcaggctt tcagccgcgc
     2821 gaataaaggc ggcggaaaaa tccacgccga tgacttcctg gcagaagcgg ctcagttcga
     2881 aggtggaacc gccgaccgcg cagccgagat cgagcgcgcg ggcggagcgg ggaagacggt
     2941 cgaagttcaa cagctcgcgc acgcagcgca ggggaaactc ggcggcgttc tccggccccg
     3001 gcagggagcc gagcgcgtcg cgggcggtgc cgtaatgaaa cagcacatac gcggcgagca
     3061 ccggcgggtc ttcgtaggga ttttccacgg cggttgggac gcggggagcg ggggcgcggt
     3121 tacgcttcga gcaggcggtt caggtaatcg gcgggttcgt agtgttcgag caggacggtg
     3181 gtctggccgc cgcggcgcat ccgggcctgg gcgaggatga cttcgggcgt tttgtccggg
     3241 gcgaacagga tgtcatc
//
LOCUS       DATDAY010000309         2207 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_763468, whole genome shotgun sequence.
ACCESSION   DATDAY010000309 DATDAY010000000
VERSION     DATDAY010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2207)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2207)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2207
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_763468"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(50..265)
                     /locus_tag="VIS74_06170"
     CDS             complement(50..265)
                     /locus_tag="VIS74_06170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792866.1"
                     /translation="MKRTAQVALEAGNSPKKGVPKASGMDVLTAPARSAYAQAFRAGI
                     PRMDAGRDAEICVYPVIRGLKWRRFTH"
     gene            complement(374..1171)
                     /locus_tag="VIS74_06175"
     CDS             complement(374..1171)
                     /locus_tag="VIS74_06175"
                     /inference="COORDINATES: protein motif:HMM:NF016236.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ family lipoprotein"
                     /protein_id="HEY5792867.1"
                     /translation="MKIHPCFLSILLIGGLAPLLTSCASRTQPEPAAPAAAGPANSEG
                     KSVQAAADDLNEYEAVAIADPLESANRATFWLNHQLYTFLLRPISKTYEKVVPKPVRT
                     GVYNAFDNVKFPVRFVNDTLQGNFKRAGQETGRFVVNSVMGFGGIVRQSDRFPALADV
                     PAGETGQTFAKWGVGHGTYVVLPVLGPSSLRDTVGLAGDYALNPVTWVSIVFGSYAWI
                     VAIPATDTTSALPGKFSQYDAATQNTLDPYLAARSSYAQSRKEAASK"
     gene            complement(1168..1746)
                     /locus_tag="VIS74_06180"
     CDS             complement(1168..1746)
                     /locus_tag="VIS74_06180"
                     /inference="COORDINATES: protein motif:HMM:NF017319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY5792868.1"
                     /translation="MNLRPLLLASLILFHQAAAASTDEAEKRLQTAVNEVLIAADRAA
                     DRGALAENLRPILQNHISFSAMTRRAIGPGWRQFTEDQQKKATQLFTTLIIRTYSSKL
                     TPGEHPVIQFKAASSPAPGRVEAPTTLLYQGSRYEVVYRLEEAEGWRITDVVIEGVSL
                     IANYRTQFDAQFKKGGASTVISALTQSVAAPQ"
     gene            complement(1806..>2207)
                     /gene="mlaD"
                     /locus_tag="VIS74_06185"
     CDS             complement(1806..>2207)
                     /gene="mlaD"
                     /locus_tag="VIS74_06185"
                     /inference="COORDINATES: protein motif:HMM:TIGR04430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HEY5792869.1"
                     /translation="LELAVGIFVLLGLAAVAYLTIKLGSGSLVSGDTYLIEARFANAG
                     GLHAGSSVLLAGVTVGRVEEVRMDSADYSAIATIRVSSRLRLPADSMASIKTAGLIGD
                     KYVSLSPGADETYLAPGTRITMTESAVDLES"
BASE COUNT          397 a          642 c          728 g          440 t
ORIGIN      
        1 caccgtgccg ttcgccatag gtgtaaccgt gtcactttct gtgtcgatgt caatgggtga
       61 aacgcctcca ttttaaaccg cggatgacgg gatagacgca gatttctgca tccctgcccg
      121 cgtccatccg cggaatcccc gcccgaaacg cttgcgcgta tgcgctgcgg gcgggtgcgg
      181 ttaaaacatc cattccgctg gcttttggca cacccttttt ggggctgttt ccggcttcca
      241 gagcgacttg cgcggtgcgc ttcatctccg ccgtgtggat gcgagcagaa cgaatgccgc
      301 cgcccgttat gaacccattc ctcccccctc ggcttgccat tcgagtcaga atcgccggac
      361 ggcgcccgcc gatttacttg gaagcggcct ctttgcgaga ttgggcgtag gaactgcggg
      421 cggccaggta ggggtccagg gtattttggg tggcggcgtc gtattgggaa aacttgccgg
      481 gcagggcgct tgtggtatcg gtggcgggaa tcgccactat ccaggcatag ctgccaaaca
      541 cgatcgagac ccaggtcacc gggttcaacg catagtctcc ggccaggccc acggtatccc
      601 gcaggctgct cgggcccaga acgggcagca cgacgtaggt gccgtggccg acgccccatt
      661 tggcgaaggt ttggccggtt tccccggcag ggacatcggc cagggccgga aagcggtcgg
      721 actggcgcac gatcccgcca aaacccatga cgctattgac gacgaaccgg ccggtttcct
      781 gcccggcgcg cttgaaattc ccctgcaagg tgtcgttcac gaaacgaacc gggaacttca
      841 cgttgtcgaa ggcgttatag accccggtgc ggaccggttt gggcaccact ttctcgtagg
      901 ttttggaaat ggggcgaagc aggaaggtgt agagctgatg gttcagccaa aaggtggccc
      961 ggttcgccga ctccaaggga tcggcaatcg ccacggcctc gtattcattg agatcgtcgg
     1021 ccgccgcctg gaccgatttc ccctcgctgt tggcggggcc ggccgcggcg ggggccgccg
     1081 gctctggctg agtccgggag gcgcagctgg tcaggagggg ggctaaccct ccgatgagaa
     1141 gaatggacag gaagcaggga tggattttca ttgcggtgcg gccacggatt gggtgagtgc
     1201 gctgatgacg gtgctggcgc cccctttttt aaactgagca tcgaattggg tccggtagtt
     1261 ggcgatcagg ctgactccct cgatcaccac atcggtaatg cgccagccct cggcctcctc
     1321 caggcggtaa acgacctcat agcggctgcc ctggtaaagc agggtcgtcg gcgcctcgac
     1381 ccggccggga gcgggggagg acgcggcttt gaattggatg acgggatgct cgccgggagt
     1441 gagcttgctg ctgtaggtgc ggatgatcag cgtcgtgaag agctgggtgg cctttttttg
     1501 ctgatcctcc gtaaactggc gccagcccgg gccgatggcc cggcgggtca tggcgctgaa
     1561 gctgatgtgg ttttgcaaaa ttggacgcag gttctccgcc aaagcgccgc gatcggcggc
     1621 gcgatcggcg gcaatgagaa cctcattcac ggcggtttgc aggcgctttt ccgcttcgtc
     1681 cgtcgaggcg gcggccgcct ggtggaacag gatcaaggaa gcaaggagca gcgggcgtag
     1741 attcacggtg cgtttttttt gtcttcgttt tccttatcga cgttgccgaa agccatttta
     1801 ccaattcacg actccagatc gaccgcggat tcggtcatgg tgatccgggt cccgggagcc
     1861 agataggtct catccgctcc gggagaaagg gatacgtatt tgtcgccgat cagtccagcg
     1921 gttttgatgg aggccatgga atccgccgga agccgaagcc gggaagagac tcggatcgtg
     1981 gcgatggcgc tatagtcggc ggaatccatc cgcacctcct ccacccgtcc gacggtgacg
     2041 ccggccagga gcacgctgct gccggcatgg aggccgcccg cattggcaaa gcgggcttcg
     2101 atgagatagg tgtcgccgct caccagcgag cccgagccga gtttgatcgt caggtaggcc
     2161 accgccgcga gtcccagcag cacaaagatc cctacggcaa gctcaag
//
LOCUS       DATDAY010000310         2725 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_2224160, whole genome shotgun sequence.
ACCESSION   DATDAY010000310 DATDAY010000000
VERSION     DATDAY010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2725)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2725)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2725
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2224160"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..669)
                     /locus_tag="VIS74_06190"
     CDS             complement(<1..669)
                     /locus_tag="VIS74_06190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEY5792870.1"
                     /translation="MSLDLLSLPDASGHFGSYGGCFVPETLMTALQELTREYETARKD
                     PDFQKELAYLLSDFCGRPTKLYFAERLTQHLGGAKIYLKREDLLHTGAHKINNAIGQI
                     LLAKRMGKTRIIAETGAGQHGVATATVAARFGCECVIYMGAVDMKRQALNVSRMEFLG
                     AKVVPVTAGQATLKEAVNEAMRDWVTNVRSTHYSLGTAYGAHPYPMMVRDFHRVIGEE
                     ARRQI"
     gene            complement(666..1661)
                     /locus_tag="VIS74_06195"
     CDS             complement(666..1661)
                     /locus_tag="VIS74_06195"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000701 - purine-specific mismatch base
                     pair DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEY5792871.1"
                     /translation="MSRKGGRHADTLDKAAFRRNLIDWFKREGRDLPWRRTRDPYAIL
                     VSEFMLQQTQVATVLPYYERWLKRFPTLAALAAADEQTVLSHWQGLGYYTRARNLHRL
                     ARGIREFPRGLAEWERLPGIGPYTAAAVAAFAFDDPAPVVDGNIARVLARLLDYRDPI
                     DTTAGKQFLKEAATALQPASRGGAYNSALMELGALICRPAPDCLICPVRKFCQATDPA
                     ALPVKQRKMKIEPRVEHRAFVADERRVWLQPAEGRQLRGFWLLPEIPAPAEEPVATIR
                     YTITRFPTRMHLHLKPAWPETTPFALDELAELPIPTPHRRAIAAARDFVHSRSSS"
     gene            1638..2609
                     /gene="rnhC"
                     /locus_tag="VIS74_06200"
     CDS             1638..2609
                     /gene="rnhC"
                     /locus_tag="VIS74_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HEY5792872.1"
                     /translation="MPPALTTHTQPLTAAQAEQLKRVLAERGFAFEAKPYTLFSARKD
                     KLSIAVYEKGPKAVLQGKGLEDFITFVLEPEVLGEARLGYEEVRDPEKFAPHFGIDES
                     GKGDFFGPLVIAGVYVNGALAKRFQEFGIRDSKAITSDASIRKLAKLIRESGAAQSVI
                     VIGPEKYNQLYTKIGNLNRLLAWGHARIIENLCELQPDCPRALSDKFADARVLERSLM
                     EKGRGILLDQRTKAESDFAVAAASILAREKFIDWMDQKGFELNLGASLPRGASAAVKK
                     QAAKIVEIYGEQALKTVAKMHFKTAYEVLGLPVPERPVFVRRTPKSA"
BASE COUNT          494 a          822 c          911 g          498 t
ORIGIN      
        1 aatctgccgc cgcgcttcct cgccgatcac ccgatggaaa tcgcgcacca tcatgggata
       61 cggatgcgcc ccgtaggccg ttccgagact gtagtgcgtt gagcggacgt tcgtcaccca
      121 gtcgcgcatg gcttcgttca ccgcttcctt cagcgtggcc tgtcccgcgg tgacggggac
      181 aaccttggcg ccgaggaatt ccattcgcga gacgttgagc gcctggcgct tcatgtccac
      241 cgcgcccatg taaatcacgc actcgcagcc gaagcgcgcg gccaccgtcg ccgtggcgac
      301 gccgtgctgg ccggcgccgg tttcagcgat gatccgggtt ttgcccatcc gtttggcgag
      361 caggatttgg ccgatggcgt tattgatctt gtgcgccccg gtgtgcagca gatcctcgcg
      421 cttgaggtaa atcttcgcgc cgccgaggtg ctgcgtcagc cgctcggcga aatagagctt
      481 ggtcggccgc ccgcagaagt cggagagcag ataggcgagc tccttttgaa agtccgggtc
      541 cttccgcgcg gtttcgtatt cgcgggtgag ctcctgcagc gccgtcatga gcgtttcggg
      601 cacaaagcag ccgccatagg agccaaaatg gccggaggca tcgggcaggg ataacaaatc
      661 gagactcatg aggaagatcg actatgcacg aaatcccgcg cggcggcaat cgctctgcga
      721 tgcggcgtgg gaatgggcag ctccgccagc tcgtcgaggg caaagggcgt ggtctccggc
      781 caggcgggct tcaaatgcag gtgcatgcgg gtcgggaagc gcgtgatggt gtaacggatc
      841 gtcgcaaccg gctcctccgc gggcgccggg atttccggca gcagccagaa tcccctcagc
      901 tggcggcctt ccgcgggctg gagccagacg cgccgctcgt cggcgacgaa cgcccggtgc
      961 tccacgcgcg gttcgatttt cattttccgc tgcttgaccg gaagcgcggc cggatcggtt
     1021 gcctggcaga atttccgcac cgggcagatc aaacaatccg gcgcaggccg gcagatcaat
     1081 gcgccgagtt ccatcaatgc ggaattatac gcgccgcccc ggctggcggg ttgcagcgcc
     1141 gtggcggcct ccttcaggaa ttgctttccg gccgtggtgt cgatgggatc gcggtaatca
     1201 agcaaccgcg ccagcacgcg ggcgatgttg ccatccacca ccggcgcggg atcgtcgaac
     1261 gcgaaggccg ccaccgccgc cgccgtgtag gggccgatgc cgggcaggcg ctcccattcc
     1321 gccaggccgc gggggaattc ccggatgccg cgggcgaggc ggtggagatt gcgcgcgcgg
     1381 gtgtaatagc cgagcccctg ccagtggctg agcaccgtct gctcatcggc ggcggccagc
     1441 gcggcgaggg tggggaagcg cttcagccag cgttcgtaat acgggaggac cgtcgcgacc
     1501 tgcgtctgct ggagcatgaa ctcggagacg aggatcgcgt agggatcgcg ggtgcggcgc
     1561 cacgggagat cgcggccctc gcgcttgaac caatcaatga gattgcggcg gaacgcggct
     1621 ttatccagag tgtcggcatg ccgcccgccc ttacgactca tacgcagccc ctgaccgccg
     1681 cgcaggcgga gcaattgaag cgggtgttgg cggaacgcgg ctttgcattc gaggccaaac
     1741 cttacaccct tttttccgcc cggaaagaca agctttccat cgccgtttac gagaaggggc
     1801 cgaaggctgt gctgcaaggg aaagggctgg aggattttat caccttcgtg ctggaacccg
     1861 aggtgctcgg cgaagcgcgg ctgggctacg aggaagtgcg tgatccggaa aaattcgcgc
     1921 cgcatttcgg catcgatgaa agcggcaagg gcgatttctt cgggccgctc gtgatcgccg
     1981 gagtttacgt gaatggcgcg ctggcgaaac gattccagga attcggcatt cgcgacagca
     2041 aggcgatcac gtcggatgcg tccattcgca agctggcgaa attgatccgc gaatccggcg
     2101 cggcgcagag cgtcatcgtg atcggcccgg agaaatacaa ccagctctac acgaagattg
     2161 gcaacctcaa ccgcctgctc gcctgggggc atgcgcgcat catcgaaaac ctgtgcgagc
     2221 tgcagccgga ctgcccgcgg gccctgtcgg acaaattcgc ggacgcgcgg gtgctcgaac
     2281 ggtcattgat ggagaaaggg cggggcatcc ttctcgatca acgcaccaaa gcggaatcgg
     2341 acttcgcggt cgccgcggct tcgatccttg cgcgcgagaa gttcattgat tggatggacc
     2401 agaagggctt cgaactgaac ctcggcgcgt ccctgccgcg gggcgcttcc gccgccgtca
     2461 aaaagcaggc ggcgaagatc gtcgaaatct acggcgagca ggctttgaaa accgtcgcga
     2521 aaatgcattt caagacggcg tatgaggttt tgggattgcc cgttcccgag cgtccggtat
     2581 ttgtgcggcg aacgcccaaa agcgcatgat cgattttggc aaacccttcg ccggcggctg
     2641 gcagtgcctg gtgcggcggc tgtttgcgcc gtttgatttc agcaaatggc tgacgctggg
     2701 tttcaccgcg tttctggcca gcttt
//
LOCUS       DATDAY010000311         3094 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_1520173, whole genome shotgun sequence.
ACCESSION   DATDAY010000311 DATDAY010000000
VERSION     DATDAY010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 3094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3094)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3094
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1520173"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            261..2207
                     /gene="acs"
                     /locus_tag="VIS74_06205"
     CDS             261..2207
                     /gene="acs"
                     /locus_tag="VIS74_06205"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF001208.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HEY5792873.1"
                     /translation="MSKTIESHLHENRVFKPSPEFSKAAHIGRLAEYRKLYDESIRQP
                     EKFWGKQAALLTWQKKWTKVLDWKMPFAKWFVGGRLNVSENCLDRHLTGARRNKAAII
                     WEGEPGEKRTLTYQQLHREVCKFANVLKRNGVKKGGRVIIYLPHIPEAAIAMLACARI
                     GAVHSVIFGGFSAESIKDRLADCGAATIITADGSYRRGQIVQLKQNIDHALKGNTTVK
                     KVIVFRRANLDIHIEEGRDVWWHREMDYVDANSPAAGFDSEHPLFILYTSGSTGKPKG
                     ILHTCGGYLLGSALTTKYTFDLKEEDIYWCTADVGWITGHSYVVYGPLALGATVFMYE
                     GAPNWPEANRFWQLIEEYQINIFYTAPTAIRSFIKWGNEWPAKHDLSSLRLLGTVGEP
                     INPEAWMWFHEKIGGGRCPISDTWWQTETGAHMITPLPGATPLKPGTATLPFFGVDAA
                     IVDDNGKEVGPNEGGKLIIRKPWPSMLRTIYGDKARYKKQYWSEVKGCYFTGDGARRD
                     KDGYFWIVGRIDDVLNVAGHRLGTAEVESALVAHEAVAEAAVVGRPDEIKGQGVVAFV
                     TLKTGQTPSDDLKNQLRKHVGEMIGPIAKPDDIRFTEALPKTRSGKIMRRLLKEVAGG
                     GTVTGDTTTLEDLSVLAKLRKTDE"
     gene            2314..2736
                     /locus_tag="VIS74_06210"
     CDS             2314..2736
                     /locus_tag="VIS74_06210"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEY5792874.1"
                     /translation="MSTEKATVSTPIQVMYYDTDAGGVVHNIVYLRFIEYARTLLAMQ
                     LGMSFAEIRRTQIHPVVVRTEIDYRRPAVLGDELTINGRLTEVSGVRFWVEFEIVRPA
                     DGAQLVTCKQALALVQMPAGRPVRLPKGFPENFELEAA"
     gene            2733..>3094
                     /locus_tag="VIS74_06215"
     CDS             2733..>3094
                     /locus_tag="VIS74_06215"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEY5792875.1"
                     /translation="MSESRKWLLWLIAASLALQVALAGWGDLYNETDGQYAGAARVMA
                     EGGSWLIPENNGVPRLVKPPPLVWLMAGSMKVFGVNEFAARLPGALAVTGWVAVTFLF
                     AARLGGPQMGFVAGVILLT"
BASE COUNT          619 a          999 c          936 g          540 t
ORIGIN      
        1 gcgtccatcg gttgaaacga cggcaatgcc gatcggggca tgctcaaacg cgctccgata
       61 cacctcatcc cgcccggcct gggaggaagg ggccgccgcc ggccggttct tgccatccgt
      121 ggcattgttc tgggtgcgat ccatggatgg agcttgactt taagcggttt cgcgggggac
      181 ggcgagggga atccatccgg atggaagtta ggattgtcga aagagggtgg atttgcttac
      241 ttaccagctt ttcctccacc atgagcaaaa ccatcgagtc ccacctgcac gagaaccggg
      301 tgttcaagcc ttccccggaa ttttccaaag ccgcccacat cggacgcctg gccgaatacc
      361 gcaagctcta cgatgaatcc atccggcagc ccgaaaagtt ttggggaaaa caagccgcgc
      421 tgctcacgtg gcagaaaaaa tggacgaagg tgctcgactg gaagatgccc ttcgccaagt
      481 ggttcgtcgg cggcaggctc aacgtcagcg agaactgcct ggaccgccac ctcaccggcg
      541 cccggcgcaa caaggcggcc atcatctggg aaggtgagcc gggcgaaaag cgcacgctga
      601 cctaccagca gctgcaccgc gaagtctgca aattcgcgaa cgtcctcaaa cgcaatggcg
      661 tcaaaaaagg cggccgcgtc atcatttatc tgccgcacat tcccgaggcg gccatcgcca
      721 tgctggcctg cgcgcgcatc ggcgcggtgc attccgtcat cttcggcggc ttcagcgccg
      781 agagcatcaa ggaccgcctg gccgactgcg gggcggcgac catcatcacc gccgacggca
      841 gctaccgccg cgggcagatc gttcagctca agcagaacat cgaccacgcg ctgaagggga
      901 atacgaccgt caaaaaagtc atcgtcttcc gccgagccaa tctcgacatc cacatcgagg
      961 aaggccgcga tgtctggtgg catcgggaaa tggactacgt cgatgccaac tccccggccg
     1021 ccggctttga cagcgagcat ccgcttttca ttctctacac cagcggttcg accggaaaac
     1081 cgaagggcat cctgcacacg tgcggcggtt acctcctcgg ctcggcgctg acgaccaaat
     1141 acaccttcga tctcaaggaa gaggacatct actggtgcac cgccgacgtc ggctggatca
     1201 ctggccacag ctacgtcgtt tacggcccgc tcgccctcgg cgccacggtc ttcatgtacg
     1261 aaggcgcgcc gaactggccg gaagccaacc ggttctggca gctcatcgag gaataccaga
     1321 tcaacatttt ctacaccgcg ccgaccgcca tccgttcctt catcaagtgg ggcaacgaat
     1381 ggccggcaaa gcacgacctc tccagcctgc ggctgctcgg cacggtgggt gaaccgatca
     1441 atcccgaagc ctggatgtgg ttccacgaaa aaatcggcgg cggccgctgc ccgatttccg
     1501 atacctggtg gcagaccgaa accggcgcgc acatgatcac gccgctgccc ggcgccaccc
     1561 ccctcaagcc gggaaccgcc acgctgccgt ttttcggcgt cgatgccgca atcgtggacg
     1621 acaacggcaa ggaagtcggg ccgaacgagg gcggcaagct gatcatccgc aaaccctggc
     1681 cctccatgct gcgcacgatc tacggcgaca aggcgcgcta caaaaagcag tattggagcg
     1741 aggtgaaggg ctgttatttc accggcgacg gcgcccgccg cgacaaggac ggttatttct
     1801 ggatcgtcgg acgcattgac gacgtgctca acgtcgccgg ccaccgcctc ggaaccgccg
     1861 aagtcgaaag cgcgctcgtg gcccatgaag ccgtggccga ggccgcggtc gtcggccggc
     1921 ccgatgaaat caagggccag ggcgtggtcg cgtttgtcac tttgaagacc ggccagaccc
     1981 ccagcgacga tctcaagaac cagctgcgca agcacgtggg cgagatgatt ggccccatcg
     2041 ccaagccgga tgacattcgc ttcaccgagg cgctgcccaa gacgcgcagc ggcaaaatca
     2101 tgcgccgcct gctcaaggaa gtcgccggcg gcggaaccgt gaccggggat accaccacgc
     2161 tggaggatct cagcgtgctg gcgaaactcc gcaagacgga cgagtgagtt ctaaatgacg
     2221 aaggacgaag cccgaatgac gaattttacc ttcgggtttc gccattcgga tttcgtcatt
     2281 ttccttctct ttcccgaggg ttaatctgcc ctcatgtcca ccgagaaggc cactgtcagc
     2341 acgccgatac aggtcatgta ttacgacacc gatgccggtg gcgtggtgca taacattgtc
     2401 tatctgcgct tcatcgaata cgcccgcacc ctgctggcca tgcagctggg catgagcttt
     2461 gcggaaatcc ggcgcacgca gattcatccg gtcgtcgtgc ggaccgagat tgactatcgc
     2521 cgtcccgccg tgctcgggga cgaactcacc atcaatggcc gcctcaccga agtctccggc
     2581 gtccggttct gggtggaatt tgaaatcgtc cgtcccgccg acggcgccca gctcgtgacc
     2641 tgcaagcagg cgctcgcgct tgtgcagatg cccgccggcc ggccggtgcg cctgcccaag
     2701 ggatttcccg agaacttcga gctcgaagcc gcgtgagcga aagccgcaag tggctgctgt
     2761 ggctgatcgc cgcatccctc gccctgcagg tggccctggc gggctggggc gatctctaca
     2821 acgaaaccga cggccaatac gccggggccg ctcgcgtcat ggccgaaggc ggctcctggc
     2881 tgatcccgga aaacaacggc gtgccgcgac tggtgaagcc gccgccgctc gtctggctca
     2941 tggccggctc gatgaaggtt ttcggcgtca atgaatttgc cgcccgcctg cccggcgcgc
     3001 tggccgtgac gggctgggtg gccgtgactt tcctgttcgc cgcgcggctc ggcggaccgc
     3061 agatggggtt cgtggcgggc gttattttgc tgac
//
LOCUS       DATDAY010000312         5436 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_1871163, whole genome shotgun sequence.
ACCESSION   DATDAY010000312 DATDAY010000000
VERSION     DATDAY010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 5436)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5436)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5436
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1871163"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            82..1383
                     /locus_tag="VIS74_06220"
     CDS             82..1383
                     /locus_tag="VIS74_06220"
                     /inference="COORDINATES: protein motif:HMM:NF019040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEY5792876.1"
                     /translation="MNPHLRTRREFLRTSVLGGALSWTLPAFLENTFGVLDAMAADSA
                     IQTVTGKDGPILVVLQLAGGNDGLNTVIPFGDDAYYSARPKIGIPAKDVRRIDDYTGF
                     HPGLKGFEAMMKEGRLSVIQGVGYPNPNRSHFRATEIWQTASDSEQTLGTGWLGRYFD
                     NACAGEDAACGISVSGETPQAFTNKGSKGISFNNPQDYQFKGDLSGKNEDYFEMANFA
                     EGEGGSIAMLDNGAASVMTASETPVSFLRRTSLNAQVNTDRIQQLLKRTPDDAKYPGG
                     RLANSLRTVARLIQGGMPTRVYYVSHGGFDTHRSQAIAHANLMRDLDESLAAFTQDLR
                     TQGNDGRVLLLTFSEFGRRVAENASGGTDHGAAAPMFLIGPRIKPGLIGAPPSLTDLH
                     RGDLRHHVDFRSVYAAVLENWLKVPSQAVLGKKFTPAPIFA"
     gene            complement(1358..2518)
                     /locus_tag="VIS74_06225"
     CDS             complement(1358..2518)
                     /locus_tag="VIS74_06225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792877.1"
                     /translation="MKPRSIYPVLLALFLGAVITRECVSGMLQPAERAYRAWLQEAII
                     PNPQPRVTVITSDDASRLLNPLDVALFLRAAMQFGADVAALSVYELDSGDGELLKSVL
                     SRESPTGFVAGALLSKRDQQADPVNFQQADWPAADALPAYSGVWNSLPVVPSITYGFL
                     NLSEHGAAETVPLFAKWQEQTVASFSLVCLTRYLSANGVELLPTSGPSALRFSNQCRI
                     PVSRNATVPLSLEVYAQIPHYRLGDLLLAAEKSEQELSMADPALPAAIKDRIVVLIPA
                     PTEQEAAAETLTLGQFQALGIASLADQLRLPAYGLWVWAVPFLWFFPWLKITEKWRNN
                     GLPLALLLLSLYGLAATSLASAAGLLLPGIPALAWAAALLLIFWIRQKWERA"
     gene            complement(2515..4182)
                     /gene="recN"
                     /locus_tag="VIS74_06230"
     CDS             complement(2515..4182)
                     /gene="recN"
                     /locus_tag="VIS74_06230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEY5792878.1"
                     /translation="MSATLTSLRIRNLALVEDLEWTPGAGFNAITGETGAGKSILIGA
                     LKLLLGERAEKSLIRAGAEQCLVEAIFELSEEAAISRRLDAAGLEPCEGGQLLLKRSL
                     TASGSRQFVNGSVCMLNVLRQLGDDLVDLHGPHDHQSLFSREQQTALLDAFAGAAEEL
                     KAYLGARAQWAKLRQEKEGDGGGGPAIEREMALLEHQIEEIETAALDEAEEEALVARH
                     RAAANSQRLAELCAGLVSLLNENEAAVFSQLADGLRQSREVARLDPRMETLPEQFAVL
                     SESVADLSREIEHYSSGLETDPAALMEMQERLDLLGKLKRKYGATLAEVVAHAEDCRG
                     KLAGMRERESRRATLDADIQSARDAAEKAAQLLSAKRGKGVKKLAGAVTAHLRDLGFR
                     QAYFTIQLEAQDHLPAAGAELAEFVFAPNPGEPPQPLRQIASSGEISRVMLALKSALA
                     GQEQTPLMVFDEIDANVGGEIATKVAAKMGELGARHQVLCITHLPQVAAAAQRQFVVE
                     KEVAGGRSFTALREVAGAAREEELARMLGGKQQSALDHARTLLGAHA"
     gene            4222..5007
                     /locus_tag="VIS74_06235"
     CDS             4222..5007
                     /locus_tag="VIS74_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY5792879.1"
                     /translation="MADFKITFLGTGTSHGVPVIGCDCAVCLSSDPRDNRLRTSVYIE
                     TPECCFVVDTTPDFRQQCLRENLRRVDAVIYTHPHSDHILGFDDLRRFCEMEDRHMPI
                     YASPGTMANLKRVFQYAFSAEGIFRTYVRPEPVEFHGEFHLGETRIVPVELPHGRCVV
                     NGFVFYRHGRKLLAYFTDCHAVPPAALAEARDAEVLVLDALRYHAHSTHLNVETALMV
                     SEQIAAGRTYFVHMCHDLGHEITEAKFPQGVKLSYDGLQIELG"
     gene            4994..>5436
                     /locus_tag="VIS74_06240"
     CDS             4994..>5436
                     /locus_tag="VIS74_06240"
                     /inference="COORDINATES: protein motif:HMM:TIGR03790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03790 family protein"
                     /protein_id="HEY5792880.1"
                     /translation="MNWANGIAALAAAFAVLAGASARADPPLPPLTVVVYNSLDPASA
                     ALAEFYARKRGIPAANVFGLPLPSEEEISRETFAEKFAAPFREQMARRGLWKLRGREA
                     IDSRIRYAVLMRGVPLKIRSAAPPLPPEQKRDPLRDRDEASVDSEL"
BASE COUNT          984 a         1856 c         1662 g          934 t
ORIGIN      
        1 gatggatctg gaaaacccca ccgactccca agccgccgaa ctgtttcatc tgatgatgag
       61 cacacccgaa taccaactga catgaatccg cacctgcgca cccgccgcga attcctccgc
      121 acctccgtcc tcggaggcgc gctctcctgg acgctgcccg cgttccttga aaacaccttc
      181 ggcgtgctcg acgccatggc cgcggattcc gccattcaga ccgtgaccgg gaaggatggc
      241 cccatcctcg tcgtgctcca gctcgccggc ggcaacgacg ggctgaacac cgtgattccc
      301 ttcggcgacg acgcctatta ttccgcccgc ccgaagatcg gcattcccgc caaggacgtg
      361 cggcggattg acgattacac cgggtttcat cccgggctca agggtttcga ggccatgatg
      421 aaggaaggaa ggctttcggt catccaaggc gtggggtatc cgaatccgaa ccgctcgcat
      481 ttccgcgcca ccgaaatctg gcagaccgcc agcgattccg aacaaaccct gggcaccggc
      541 tggctgggcc ggtatttcga caacgcctgc gcgggcgagg acgcggcctg cggcatcagc
      601 gtttccgggg agactccgca ggccttcacc aacaaaggca gcaagggcat ctcgtttaac
      661 aatccgcaag actaccagtt caagggcgat ctcagcggca agaacgaaga ctattttgaa
      721 atggccaact tcgccgaggg agaaggcggc agcatcgcca tgctcgacaa cggcgcggcc
      781 agcgtcatga cggcctcgga aacccccgtg agtttcctgc gccgcacgtc cctgaacgcc
      841 caggtcaaca cggaccgcat tcagcagctc ctcaagcgga cgccggacga cgccaaatac
      901 cccggcggcc ggctcgccaa ctcgcttcgc acggtggccc ggctcattca gggcggcatg
      961 cccacgcggg tgtattacgt cagccacggc ggattcgata cgcaccgcag ccaggccatc
     1021 gcgcacgcca atctcatgcg cgacctggac gagtcgctgg ccgcattcac gcaggatttg
     1081 cgcacccagg gaaatgacgg gcgcgtgctg ctgctcacct tcagcgaatt cggccgccgc
     1141 gtcgccgaaa acgccagcgg cggcacggat cacggcgccg cggccccgat gttcctcatc
     1201 ggcccccgca tcaaacccgg cctgatcggc gcgcctccca gcctcaccga cctccaccgc
     1261 ggcgatctcc gccatcacgt ggacttccgg tcggtctatg cggcggtgct ggaaaactgg
     1321 ctgaaggttc ccagccaggc ggtgctgggg aaaaaattca cgcccgctcc catttttgcc
     1381 tgatccaaaa aatcaggagc agcgccgccg cccaggccag ggcggggatg cccggcagca
     1441 gaaggcccgc cgccgacgcc aggctcgtcg ccgcgaggcc atacaggctc agcagcagga
     1501 gcgccaacgg caggccgttg ttccgccatt tttcggtgat cttcagccag gggaaaaacc
     1561 agagaaaggg aaccgcccac acccacaacc cataggcggg gagccgcagc tgatccgcca
     1621 gcgaggcgat cccgagcgcc tggaactggc cgagggtaag cgtttccgcg gcggcctcct
     1681 gctcggtcgg ggcgggaatc agcaccacga tccggtcctt gatcgccgcc ggcagcgccg
     1741 ggtcggccat cgacagctcc tgctcgcttt tttccgccgc cagcagcaaa tcccccaggc
     1801 ggtaatgcgg gatttgcgcg taaacttcca gcgaaagcgg aacggtcgcg ttccgggaaa
     1861 ccggaatccg gcattgattg gaaaaccgga gcgccgaggg gccggaggtg ggcaaaagct
     1921 ccacgccgtt cgccgacaaa tagcgggtca ggcaaaccag ggaaaacgag gccacggttt
     1981 gctcctgcca ttttgcgaac agcggaacgg tctccgccgc cccgtgttcg gaaagattca
     2041 gaaaaccgta ggtgatcgat ggaacgacgg gcaggctgtt ccaaacgccg gagtaggcgg
     2101 gcagcgcgtc ggcagcgggc cagtccgctt gctggaaatt cacggggtcc gcctgctggt
     2161 cccgtttgct gaggagcgcg ccggccacaa accccgtcgg cgactcgcga gagagcacgc
     2221 tttttaataa ctccccatcc cccgaatcca gctcataaac cgagagggcc gccacgtccg
     2281 cgccgaactg catcgccgcg cgcaggaaga gcgccacgtc cagcgggttc aaaagccggc
     2341 tggcgtcatc cgaggtgatg accgtcacac gcggttgcgg attgggaatg atggcttcct
     2401 gcagccaggc gcgatacgcg cgctcggcgg gctggagcat tcccgaaacg cattcccggg
     2461 tgatcacggc gccgaggaac agggcgagaa ggaccggata aatcgaacgc ggcttcatgc
     2521 gtgcgcgccc agcagcgtgc gggcgtggtc gagcgcggac tgctgctttc ctcccagcat
     2581 tcgggccagt tcctcctcgc gggccgctcc cgccacctcc cgcaatgcgg tgaagctgcg
     2641 cccgccggcc acttcctttt cgaccacgaa ctgccgctgc gcggcggcgg ccacctgcgg
     2701 gagatgggtg atgcaaagca cctgatgccg ggcgccgagc tcgcccatct tcgcggcgac
     2761 ctttgtggcg atctcgccgc cgacgttcgc gtcgatttca tcgaatacca tgagcggcgt
     2821 ctgctcctgt ccggccagtg cggacttgag cgcgagcatg acgcgggaga tttccccgct
     2881 ggacgcgatc tgccgcagcg gctgcggcgg ctcgccggga ttcggcgcga agacaaactc
     2941 cgccagctcc gcgccggccg cgggcagatg gtcctgcgct tccagttgaa tggtaaaata
     3001 cgcctgccgg aacccgaggt cgcgcaggtg cgcggtgacg gcgccggcga gttttttgac
     3061 gcccttcccg cgcttggcgc tgagcaattg ggcggctttc tccgcggcgt cgcgcgccga
     3121 ctggatgtcc gcatccagcg tcgcgcggcg gctttcgcgt tcgcgcatcc cggcgagttt
     3181 cccgcggcaa tcctcggcgt gcgcgaccac ctccgcgagc gtcgccccgt atttgcgttt
     3241 gagtttcccc agcaaatcca gccgctcctg catttccatc aacgccgccg gatcggtttc
     3301 caatccggag ctgtaatgtt cgatctcccg ggacagatcc gccacgcttt cggaaaggac
     3361 ggcgaattgc tccggcaggg tctccattcg cggatcgagc cgggccacct cgcgggactg
     3421 gcgcaggcca tcggccagct gggaaaagac cgccgcttcg ttctcattga gcaggctgac
     3481 gaggccggcg caaagttccg ccaggcgctg ggaattggcg gcggcgcggt gccgcgcgac
     3541 gagcgcctcc tcctcggctt catccagcgc cgcggtttcg atctcttcga tctggtgctc
     3601 caggagcgcc atttcccgtt cgatggccgg gccaccgccc ccgtcgccct ccttttcctg
     3661 ccggagtttc gcccactgcg cccgcgcgcc aaggtaggct ttcaattcct ccgccgctcc
     3721 ggcaaaggca tccagcagcg cggtctgttg ttcgcgggaa aagagggatt gatggtcatg
     3781 cgggccgtgg agatccacga ggtcgtcgcc gagttgccgg aggacattca gcatgcagac
     3841 cgatccattc acgaactgcc ggctgcccga ggcggtcagc gagcgcttga gcagcagctg
     3901 ccccccctcg cagggctcca gcccggccgc gtcgagccgg cgggagatcg ccgcctcctc
     3961 ggaaagctcg aaaatcgcct cgacgaggca ttgctcggcc ccggcgcgaa tcagggattt
     4021 ctcggcgcgc tcgcccagca gcaatttcag ggcgccgata agaatggact tccccgcccc
     4081 ggtttccccg gtaatggcat taaatccggc gcccggagtc cactccagat cctcgacgag
     4141 ggccagattt cgaatgcgca gcgaagtcaa agttgccgac atgccgggaa ctatgtcagc
     4201 ctccgggtgc aaaataaagc aatggccgat ttcaagatca cctttttggg caccggaacc
     4261 tcccacggag taccggtgat cggatgcgac tgcgcggtct gcctctcgtc cgatccccgc
     4321 gacaaccggc tgagaacgtc cgtttacatc gagacgccgg agtgctgttt cgtggtggat
     4381 acgacgccgg actttcgcca gcaatgcctg cgggaaaacc tccgccgggt ggacgccgtc
     4441 atctacaccc atccgcacag cgatcacatc ctgggcttcg acgacctgcg gcggttttgt
     4501 gaaatggagg accgccacat gccgatctac gcgtcccccg ggaccatggc caacctcaag
     4561 cgcgtgttcc aatacgcctt ctccgccgaa gggatttttc gcacctatgt ccgccccgag
     4621 ccggtcgagt ttcacggcga attccatttg ggcgaaaccc gcattgtgcc cgtcgaactc
     4681 ccgcatggcc gctgcgtcgt caacggcttc gtcttttacc gccacgggcg caaattgctg
     4741 gcctatttca ccgactgcca cgccgtgccg ccggcggcgc tcgccgaagc gcgggacgcg
     4801 gaggttctcg tgctggatgc gttgcgctac cacgcccatt ccacgcacct gaatgtggaa
     4861 accgcactga tggtttcgga acaaatcgcc gccggccgca cttatttcgt gcatatgtgc
     4921 cacgatctgg ggcatgaaat cacggaggcg aaatttcctc agggggtaaa attgtcctac
     4981 gatgggctgc agattgaatt gggctaacgg gattgccgcg ctggcggccg cattcgcggt
     5041 tctcgccggc gcaagcgcgc gggcggatcc gccgctgccg ccccttaccg tggtcgttta
     5101 caattccctc gaccccgcct ccgccgcgct ggcggaattt tacgcccgga agcgcgggat
     5161 tcccgccgcc aatgttttcg ggctgcccct gccgtccgag gaggaaattt cccgggaaac
     5221 ctttgcggaa aaattcgcgg ctccctttcg ggaacaaatg gcccggcgcg ggctgtggaa
     5281 actccgcggc cgggaagcca tcgactcccg catccgttac gcggtgctca tgcgcggagt
     5341 ccccctcaag atccggtccg ccgccccgcc gctcccgccg gagcaaaaac gcgatcccct
     5401 tcgcgaccgc gatgaagctt ccgtggattc cgaact
//
LOCUS       DATDAY010000313         2717 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_1669535, whole genome shotgun sequence.
ACCESSION   DATDAY010000313 DATDAY010000000
VERSION     DATDAY010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2717)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2717)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2717
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1669535"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..194)
                     /locus_tag="VIS74_06245"
     CDS             complement(<1..194)
                     /locus_tag="VIS74_06245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792881.1"
                     /translation="MLPNFILATTSAAVFVYILGQGGERFYTEAWGTLLLSPLIWIRL
                     APPPLNEWILRACRNGGDVI"
     gene            complement(257..1525)
                     /locus_tag="VIS74_06250"
     CDS             complement(257..1525)
                     /locus_tag="VIS74_06250"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEY5792882.1"
                     /translation="MRTQVTKLANGVRVASCPMPHVQSVAVGVWAAVGGRHESRRLNG
                     ISHFIEHLLFKGTARRSARRIMQEIEGIGGDINANTAEERTCYYASAPHEYLPQVLDV
                     LTDLYRHPRFSPADIELERGVIHEEIQMYLDEPSQHVLELLHEKFWPGQAIGRALTGT
                     AESIAKFTREDFAGYLGKHYQGAATIVTAAGNLDHGRLVESVEKSLGGLVPGKRPASP
                     PARSLLSGPELHVVTRETQQTHVAVGLPGTSYRDPDRFAANLLHVMLGGNASSRLFQE
                     LREKHGYCYSISTYPLFFSDTGMLNLSIGLDARNLAKAFQLIGRQFARIREQLGSPAE
                     LKRAKEYTIGLSRMSMERTSSQSSRIGQSLLAFGRVVDPEEIYAKIRAVTPDDIRRVA
                     GKLLGPEGACAVIIGPVKRDESLMRALMGK"
     gene            complement(1506..2177)
                     /gene="ispD"
                     /locus_tag="VIS74_06255"
     CDS             complement(1506..2177)
                     /gene="ispD"
                     /locus_tag="VIS74_06255"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: protein motif:HMM:NF013308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HEY5792883.1"
                     /translation="MSLTAVIVAAGKSRRMGFDKLRAELAGKPVLEHSLQAFQDCEDV
                     SEIIWVTAPDEDGVPLRGKITQAVPGGAERHHSVWNGLRAVTTEYVAVHDAARPLITP
                     AGISVCYELAQESGAAACAEPESDTLHRADEERLLVEPVSRANLWRMQTPQIFRTALL
                     KEAYAAVIGRGGSVTDEVGAVLAIGQKVRLWANPEWNFKITYPRDLALAEFILEQRSH
                     AHAGH"
     gene            complement(2251..2703)
                     /locus_tag="VIS74_06260"
     CDS             complement(2251..2703)
                     /locus_tag="VIS74_06260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HEY5792884.1"
                     /translation="MSLNQQIDNDLKDAMRAKDAVKLGVVRILKSAVKYAAIEKHGAE
                     GQLEDPEVVAVIRKQIKQRQDSIESFEKAGRVELAAGEKNEMAILEGYLPKALAAEEL
                     AALVAAAIAEAGATSKQQMGAVMKIANLKAAGRADGRALSAEVQKQLG"
BASE COUNT          488 a          932 c          776 g          521 t
ORIGIN      
        1 ataatcacgt ccccgccatt gcggcaggcc cgcaatatcc actcattcag cggaggcggc
       61 gccagccgga tccaaatcag cggcgagagc agcaaagtcc cccacgcttc ggtgtaaaac
      121 cgctcgcctc cctggcccag aatatacaca aaaaccgccg ccgacgttgt tgccagaatg
      181 aaattcggaa gcataagcca tggcttgaac cgctgtaacc atcctcccaa atcccgcgga
      241 tagcgagtta ttttccctat tttcccatca atgcgcgcat caatgattca tcccgcttga
      301 ccgggccaat gatcaccgcg caggcgccct ccggaccaag cagttttccg gcgacccgcc
      361 ggatgtcgtc cggggtgacc gcgcggattt tcgcgtaaat ttcctccgga tccaccaccc
      421 ggccaaaggc cagcagcgac tggccgatcc gcgaactctg cgaggaggtg cgctccatgg
      481 acatgcggct gaggccgatg gtgtattcct tcgcgcgctt caactccgcg ggcgaaccga
      541 gctgctcgcg gatgcgcgca aactgccggc cgatcaattg gaacgccttg gcgaggttgc
      601 gggcgtcgag cccgatggag agattcagca tgccggtgtc cgaaaaaaaa agtggatacg
      661 tcgagatcga ataacaatac ccgtgctttt cgcgcagctc ctgaaagagc cgcgagctgg
      721 cgttcccgcc gagcatgacg tggagcaggt tcgccgcgaa ccgatccgga tcgcggtagc
      781 tcgtcccggg aaggccgacg gccacgtgcg tctgctgggt ctcgcgggtc acgacatgca
      841 gctccggccc gctgaggagg gaacgcgccg gcggcgaagc gggccgtttc cccggaacca
      901 gcccgcccag tgatttttcc acggattcca ccaaccgccc gtggtcgaga tttcccgcgg
      961 ccgtcacgat cgtcgccgct ccctgataat gtttgcccag ataaccggca aaatcctccc
     1021 gggtgaattt cgcaatggat tccgccgtgc ccgtcaacgc ccgcccgatc gcctgacccg
     1081 gccagaattt ttcgtggagc aactccagca cgtgctgcga gggctcgtcc agatacatct
     1141 ggatttcctc atggatcacc ccgcgctcca gctcgatgtc cgccggggaa aaacgcgggt
     1201 gccggtagag gtccgtcaac acgtccagca cctgcggcag gtattcgtgc ggcgcgctgg
     1261 cgtagtagca ggtccgctcc tccgcggtgt tggcattgat gtcgccgccg atgccttcga
     1321 tttcctgcat gatgcgccgc gcgctgcggc gcgccgtgcc cttgaaaagc aggtgctcga
     1381 tgaaatgcga aatcccgttc agccggcggg attcgtggcg cccgcccacc gccgcccaca
     1441 cgccgacggc cacggactga acgtgcggca tcgggcagct ggccacgcgg acgccattgg
     1501 cgagtttagt gacctgcgtg cgcatggctc cgttgctcca aaataaattc cgccagcgca
     1561 agatcgcgcg gatacgtgat cttgaaattc cactcgggat tcgcccaaag gcggaccttt
     1621 tgcccaatcg ccagcaccgc gccgacctcg tcggtcacgc tcccgccccg gccaatgacc
     1681 gcggcgtagg cttccttcag caaggccgtg cggaaaatct gcggggtctg catccgccag
     1741 agattcgcgc gggaaaccgg ctcgacgagc aaccgctcct cgtcggcccg gtgcagcgtg
     1801 tcggattccg gttcggcgca ggccgccgcg cccgattcct gcgcgagctc gtagcaaacc
     1861 gaaatgcccg ccggcgtgat caatggccgc gccgcatcgt gaaccgccac gtattccgtc
     1921 gtcaccgccc gcaggccatt ccagacggag tgatgccgct cggccccgcc gggaaccgcc
     1981 tgggtgattt ttccgcgaag gggaaccccg tcttcgtccg gggcggtcac ccagatgatc
     2041 tcgctcacgt cctcgcagtc ctgaaacgcc tgcagggaat gctccaggac gggcttcccc
     2101 gccagctccg cgcggagctt gtcaaacccc atgcgccggc tctttccggc ggcgacgatc
     2161 acggcagtca aactcacgcg attttggatt ttggattttg gattttcgaa tggggaatca
     2221 tcgcgacggc ttacaatcga gaattgaaaa ttaccccagc tgtttctgca cctcggcgct
     2281 cagcgcccgg ccgtccgccc ggcccgcggc cttgaggttc gcgatcttca tgacggcgcc
     2341 catctgctgc ttcgaggtcg cccccgcctc ggcgatggcc gccgcgacca gcgcggccaa
     2401 ttcctccgcc gccagggcct tgggcaggta gccttccaaa atggccatct catttttctc
     2461 gccggcggcc agttccaccc ggcccgcttt ttcaaagctt tcgattgagt cctgccgttg
     2521 cttgatctgc ttgcgaatca ccgcaaccac ttccggatct tccaattgcc cttccgcccc
     2581 atgcttctcg atcgcggcat acttcaccgc cgatttcagg atgcgcacga cgcccaattt
     2641 cacggcgtcc ttggcccgca tcgcgtcctt gaggtcgttg tcgatctgtt ggtttaaact
     2701 catccctcca acctacg
//
LOCUS       DATDAY010000314         3246 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_1167296, whole genome shotgun sequence.
ACCESSION   DATDAY010000314 DATDAY010000000
VERSION     DATDAY010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 3246)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3246)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3246
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1167296"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..403)
                     /locus_tag="VIS74_06265"
     CDS             complement(<1..403)
                     /locus_tag="VIS74_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6428 family protein"
                     /protein_id="HEY5792885.1"
                     /translation="MKTTEFKSLLERHPDLPLAFVLPDGSRIPAHAHVTEVGRVDKTF
                     LDCGGDPHRDSACLLQTWVAEDLDHRLSAAKLRGIFDLAAPLLGNEELEVEVEYEAQW
                     ISQFPVESASAREGALMFQLAAKHTDCLAKES"
     gene            complement(410..904)
                     /locus_tag="VIS74_06270"
     CDS             complement(410..904)
                     /locus_tag="VIS74_06270"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF013607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HEY5792886.1"
                     /translation="MNPKSIRFQILFLCTGNSARSILAEYLLKKKAPGKFIVRSAGSH
                     PKPAPNPLALQTLREHFDIDASDARSKSWEEFAGAPFDFVITLCDNAKEACPVWPGQP
                     ILAHWGSPDPAEATGSEAERLNVFWQIAQQIQRRIELFASLPFDKLDALRLETATREI
                     GNQS"
     gene            complement(915..1265)
                     /locus_tag="VIS74_06275"
     CDS             complement(915..1265)
                     /locus_tag="VIS74_06275"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEY5792887.1"
                     /translation="MDLIQIYQCFCDANRLRILHLLAQGPLCVCHFQEILEIPQAKVS
                     QHLAYLRKHQLVETTRHGNWMIYRLPVRRPPELEKNLQCLQDCVTSDPRFREDRERRR
                     KAVACCDWLESACC"
     gene            complement(1438..3039)
                     /locus_tag="VIS74_06280"
     CDS             complement(1438..3039)
                     /locus_tag="VIS74_06280"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HEY5792888.1"
                     /translation="MNPVRQLRSLFDNYLQETTLRYPEHGSNLGFDEFRPLLSENTKA
                     IHRDQNRLVEKTLAAVEALPESAFDAGGWLDRRIFASMLRRHRIFEGELQRTKRNPQG
                     NCDAAIESIFELLVRCEGKLANALPAIESRLAKIPDYLAAGAKNIRKPVPLWVKLTEK
                     ACAGGIEFFDELGQTFQPIATPRTAALLKGAQAALKDYAQAISRKSAGPKDGFACGTA
                     VFEELMRERLGFDWSVNEAAANGRRLVAEMQALLDLEARRLGKKTAREILDAAAGEWI
                     PEKPLLSLYQTATDTVKERLRQRELMSLPPGETLVVLPVPAFLKHHFPTAAYTQPPPF
                     SKKQQGIFWVNDLSLDAAGEAEKLAEQRQHFGLELTSAHEAYPGHHVQFATQNRFASK
                     IRRLADHSISYEGWTMWCELMCVEQALVENPYARLQQLHDALWRAHRILIDAGLHTGM
                     SFDWACRTLMEGVGFTRARAEGDVNWYSSSPTVPMSYLLGRLEMERLHAHFVLKGNWT
                     LRQFNDWLLSYGAIPQRWIWQSLLR"
BASE COUNT          571 a          896 c         1138 g          641 t
ORIGIN      
        1 aggattcctt cgcgaggcag tccgtgtgtt tggcggcgag ctggaacatc agcgcgccct
       61 cccgggcgga agccgactcc acggggaatt gggaaatcca ttgggcttcg tattccactt
      121 cgacctccag ctcctcattg cccagaagcg gggccgccag gtcaaaaatg ccccggagtt
      181 tggcggcgct gaggcggtgg tccaaatctt ccgccaccca cgtttgcagc aagcaggccg
      241 agtcacggtg cggatcgccg ccgcagtcga ggaaggtctt gtccacccgg ccgacctcgg
      301 tgacgtgcgc gtgggcgggg atgcgcgagc cgtcgggcag cacgaaggcg agggggagat
      361 ccggatgccg ctcgagcagg gatttgaatt cagtggtttt cataaaggtt caggattgat
      421 tgccgatttc gcgggtggcg gtttcgaggc ggagggcgtc gagtttgtca aacggcagcg
      481 aagcgaagag ttcgatccgg cgctggattt gctgggcaat ctgccagaag acattgagcc
      541 gttccgcttc ggagccggtg gcttccgcgg ggtcgggcga tccccaatgc gcgaggatcg
      601 gctggccggg ccagaccggg caggcttctt tcgcgttgtc gcaaagcgtg atgacaaaat
      661 cgaaaggggc gccggcaaat tcctcccacg acttgctccg ggcgtccgag gcgtcgatgt
      721 cgaaatgttc gcgcagcgtc tggagggcga gcggattggg cgcgggtttg ggatgcgagc
      781 cggcgctgcg cacgatgaat tttcccggcg ccttcttttt caggaggtat tcggcgagga
      841 tgctgcgggc ggagttgccc gtgcaaagaa agagaatctg gaagcggatg gatttggggt
      901 tcataaaggg ggccttagca gcaggcgctc tccagccagt cgcagcaggc cacggccttg
      961 cggcggcgtt cccggtcttc gcgaaagcgc gggtcgctgg tcacgcagtc ctggaggcat
     1021 tgcaggttct tttcgagctc gggcggccgg cggacaggca ggcggtaaat catccagttt
     1081 ccgtgccggg tggtttccac aagctggtgt ttgcgcaggt aggcgagatg ctgggaaacc
     1141 ttggcctgcg ggatttccag aatctcctgg aaatggcaga cgcacagcgg cccctgcgcg
     1201 agcaggtgaa gaatccgcag ccggttggcg tcgcagaaac actggtaaat ctgaatcaag
     1261 tccacgggga atctatattc ggataaatga atattcgtca atgtgaatat gaatatctga
     1321 tgatggaatt aaagtcggcg gggtcggcgg ccccgccctg cccattattt ggcagggacg
     1381 gctcgtagaa gggtgagggg ctccttcccc ctcatgccgt ccgtcgcttc cggccgctca
     1441 gcgcagcagg gattgccaga tccagcgctg cgggattgcg ccgtagctca gcagccagtc
     1501 gttgaactgc ctgagcgtcc aatttccctt gagcacgaag tgcgcgtgca gccgctccat
     1561 ttcgaggcgg ccgaggagat aactcatcgg aacggtgggg ctggaactat accagttcac
     1621 atcgccttcc gcgcgggcgc gggtgaaacc cactccctcc atgagcgtgc ggcaggccca
     1681 gtcgaagctc atgccggtgt gcaggccggc atcgatgagg atgcggtggg cgcgccagag
     1741 ggcgtcgtgg agctgttgca gccgggcata gggattctcc accagcgcct gctcgacgca
     1801 catgagctcg caccacatgg tccagccttc gtaggaaatg gagtgatccg ccagccgccg
     1861 gattttgctc gcgaagcggt tctgcgtggc gaactggacg tgatggccgg gatacgcctc
     1921 gtgggccgac gtgagttcga ggccgaagtg ctggcgctgc tccgcgagtt tttcggcttc
     1981 gccggcggca tcgaggctga gatcgttaac ccagaaaatg ccctgctgct ttttgctgaa
     2041 aggcggcggc tgcgtatagg ccgcggtggg aaaatggtgc ttgaggaagg cgggcacggg
     2101 cagcacgacg agcgtttcgc cgggcggcag gctcatgagt tcccgttgcc ggagccgctc
     2161 cttcacggtg tccgtggcgg tttgatacag cgaaagcagc ggcttttcgg gaatccattc
     2221 gccggcggcg gcgtcgagga tttcccgggc ggtcttcttg cccagccggc gggcctccag
     2281 gtcgagcagg gcctgcattt cggcgacgag gcggcggcca ttggcggcgg cttcgttgac
     2341 cgaccaatca aagccgagcc gttcgcgcat gagctcctca aagactgcgg tcccgcaggc
     2401 gaaaccgtcc ttcgggccgg cgctttttcg ggaaatggcc tgcgcgtaat ccttcagggc
     2461 cgcctgggcg cccttgagca gggcggccgt gcgcggggtg gcgatcggtt gaaaggtctg
     2521 gccgagctcg tcaaaaaatt caatgccgcc cgcgcaggcc ttttcggtca gtttcaccca
     2581 gagcggaacg ggcttgcgga tgttcttcgc gccggcggcg aggtaatcgg gaattttcgc
     2641 caggcgggat tcgatggcgg gcagggcgtt cgcgagcttg ccttcgcagc ggacgagcag
     2701 ttcaaagatg gattcgatgg ccgcatcgca attgccctgc ggattgcgtt tggtccgttg
     2761 aagttcgccc tcgaaaatcc ggtgccggcg cagcatcgag gcgaagatgc ggcggtccag
     2821 ccagccgccg gcgtcgaacg ccgattcggg cagcgcctcc acggcggcga gggtcttttc
     2881 gacgaggcgg ttttgatcgc gatgaatggc cttcgtgttt tccgagagca gcgggcggaa
     2941 ttcgtcgaac ccgagattgc tgccatgctc cggataacgc agcgtggttt cctgaaggta
     3001 attgtcgaag agcgaacgga gctggcggac gggattcatg ggtggaggcg gggtggatta
     3061 accgcaaaga acgccgagag cacaaagagg tgaatttatt tccttatttc tttgcgatcc
     3121 ttgcgtgctc ggcggttaaa aatttaccgg ggcagcgccg gggcgggttc gggcgggccg
     3181 gtgatgcggc cttcgtcgtc caccttggag agcgcggtgt tcacgagctc gttgccgagg
     3241 tcgcgc
//
LOCUS       DATDAY010000315         2243 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_2227441, whole genome shotgun sequence.
ACCESSION   DATDAY010000315 DATDAY010000000
VERSION     DATDAY010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2243)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2243)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2243
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2227441"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            38..1075
                     /locus_tag="VIS74_06285"
     CDS             38..1075
                     /locus_tag="VIS74_06285"
                     /inference="COORDINATES: protein motif:HMM:NF021254.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosortase/archaeosortase family protein"
                     /protein_id="HEY5792889.1"
                     /translation="MKSPIFAPGADRRTIALLAAVWIPAALLVYLLAVQPYSSGYLYV
                     RLPILQAAAGFWNSPDWEHCGLVPLASAFILYLEKDRLASLPIRPSAGGLLVAALGFL
                     LYWAGYRVDNVYFGYASIQILLAGMILTILGWKFMGALFFPWVFLTFMWPVLFLENYL
                     AFPLRLIMSEASVTVLNAIGIPAIKSGTAILSASESVGGIAARPAGALFSVDVANPCS
                     GIRSLFALMMVSALYGYFTLASGWKRLALFFCAAPLAILGNLFRILMLTIGTLAFGAD
                     FAIGQGLEKPSTFHLAAGFVVFAVALAGMVGIHQLLEWNPRKRKTVEPPSEKPAAEIL
                     DAKRGNQEDEY"
     gene            1079..1825
                     /locus_tag="VIS74_06290"
     CDS             1079..1825
                     /locus_tag="VIS74_06290"
                     /inference="COORDINATES: protein motif:HMM:NF023410.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpsI family protein"
                     /protein_id="HEY5792890.1"
                     /translation="MNPAAAEEPRPFVFPWIPVLGFCALALATLVLCQFDYRHQTVPE
                     AGVTLNLPERLGDYRGKDVDISEGERVILPKDTEFAKKAYTDIGGDVINCQIVLSGGD
                     KRSIHRPEICLPGQGWNVSSTATMPIKLPSGKTLEVTKLRLTRPVEISPGVRKPLTMW
                     FLYWFVGDHYTTHDHLRRILRTNLDMLLHNRAHRWAYVIISAPVLEGFKFEGKNEALT
                     LGMLESFIPEIVPAFQKSEMAGLAESNPPR"
     gene            1934..>2243
                     /locus_tag="VIS74_06295"
     CDS             1934..>2243
                     /locus_tag="VIS74_06295"
                     /inference="COORDINATES: protein motif:HMM:NF012872.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite reductase subunit alpha"
                     /protein_id="HEY5792891.1"
                     /translation="MSSDPTALYSRKNPFPAALKTNLKLTGEGSEKDTRHFEIVLAGS
                     GLNYEVGDSLGVFPTNNPALVKEILAAAKFTGEELVTNPNGESVPLRQALLKDYILTE
                     V"
BASE COUNT          394 a          661 c          685 g          503 t
ORIGIN      
        1 tcggcgccgc gggggcggcc cgtgtgaaac cgaatccatg aagtccccca tttttgcccc
       61 cggcgccgac cgccggacca ttgcgctgct ggcggcggtt tggattccgg ccgctctgct
      121 ggtctatctg ctggcggtgc agccctattc ttcgggctat ctttacgtcc ggctgcccat
      181 tctgcaggcc gcggccgggt tctggaacag cccggactgg gagcattgcg gcttggttcc
      241 cctggccagt gcgttcattt tgtatctgga gaaggaccgg ctggccagcc ttcccatccg
      301 gccaagcgct ggggggcttt tggtggccgc cttgggattc ctgctctact gggcgggcta
      361 ccgggtggac aatgtttact tcgggtatgc gtccattcaa atcctgctcg cggggatgat
      421 cctcacgatt ctcggctgga aattcatggg cgcgcttttc tttccctggg tcttcctcac
      481 cttcatgtgg ccggtgctgt ttttggagaa ttacctggcg tttccgctgc ggctgatcat
      541 gtcggaagcc agtgtcacgg tgctgaatgc catcggcatt ccggcgatca agagcggcac
      601 cgccattctc tcggcctcgg aaagcgtggg cggaatcgcg gcgcggcctg ccggagcctt
      661 gttttcggtg gacgtggcca atccgtgcag cggcatccgc tcgctgtttg cgttgatgat
      721 ggtttcggcg ctgtatggct attttacgct ggcgtccggt tggaagcggc tggcgctttt
      781 tttctgcgcg gcgccgctgg cgattctggg aaatttgttc cgcattttga tgctgaccat
      841 cggcacgctg gccttcggcg cggattttgc aatcggccag ggtttggaga aaccttccac
      901 gtttcacctg gccgcgggat ttgtggtctt cgccgtcgcg ctcgcgggca tggttggcat
      961 ccatcagctg ctggagtgga atccgcgaaa gcggaaaacc gtggagcctc cctccgaaaa
     1021 gcccgccgcg gaaattttgg atgcaaaaag ggggaatcag gaggatgaat attaagccat
     1081 gaatcccgcc gcggccgaag aaccccgtcc tttcgttttt ccgtggattc ccgtccttgg
     1141 tttttgcgcg ctggcgctgg cgaccctcgt tctctgccag ttcgactacc gtcaccagac
     1201 ggtccccgag gcgggcgtga cgttgaatct gcccgagcgg ctgggggact atcgcggcaa
     1261 ggacgtggac atctccgaag gcgaacgcgt gattttgccg aaggacacgg aatttgccaa
     1321 aaaggcctac acggatatcg gcggcgatgt catcaattgc cagatcgtgc tcagcggcgg
     1381 ggacaagcgc agcattcacc gcccggaaat ctgcctgccg ggtcagggat ggaatgtcag
     1441 ctcgaccgcg acgatgccga tcaaattgcc aagcggtaag acattggaag tgaccaagct
     1501 gcggttgacg cgcccggtcg agatttcccc gggggtccgc aaaccattga ccatgtggtt
     1561 cctttactgg tttgtgggcg accattacac cacgcacgat cacctgaggc gcatcctgcg
     1621 gaccaatctg gacatgctgc tgcacaaccg cgcccaccgc tgggcctatg tcatcatcag
     1681 cgccccggtg ctggagggct tcaagttcga gggaaaaaac gaggcgctga cgctgggaat
     1741 gctcgaaagt ttcatccccg aaattgtgcc ggccttccag aagtcggaga tggcgggatt
     1801 ggccgaatcc aatcccccgc gttgacgcgc ggcttttcgc agcttggcat tctttctgct
     1861 cgtgagttgc caaagcaagc cgcccgcgca atttcccatt gccgtggcgg cggcaattcc
     1921 ttaatgcgaa atcatgtcat cggatccaac cgccctttac tcccggaaaa acccgttccc
     1981 ggccgcgctg aaaaccaatc tcaagcttac cggcgagggg tcggaaaagg acacccgcca
     2041 ttttgagatc gttctcgcgg ggtccggact caactacgaa gtgggcgatt cgctgggcgt
     2101 tttcccgacg aataatcccg cgctggtgaa ggaaatcctc gccgccgcca aattcacggg
     2161 cgaggaattg gtgacaaatc ccaatggcga gtccgtcccg ctccggcagg cgctgctgaa
     2221 ggattacatc ctcaccgaag tgt
//
LOCUS       DATDAY010000316         2650 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_816093, whole genome shotgun sequence.
ACCESSION   DATDAY010000316 DATDAY010000000
VERSION     DATDAY010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2650)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2650)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2650
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_816093"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(100..648)
                     /locus_tag="VIS74_06300"
     CDS             complement(100..648)
                     /locus_tag="VIS74_06300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792892.1"
                     /translation="MENPAPEFRGGEVRVGWNADGLWTLARMEDECIFTQATRDNEKL
                     WLLGDVFEIFVRDMAGEEYLELHAAPTGHRLQLCFASDQVIEHLRNGHLEVEELMVDE
                     PLFCLKVRTAPDCWEVLACVSAIKGRALRASFSRYDYRDADTPPVLSSTSMHREVNYH
                     RQHEWAELRLVGQEVPLRAGQG"
     gene            692..1603
                     /locus_tag="VIS74_06305"
     CDS             692..1603
                     /locus_tag="VIS74_06305"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygenase MpaB family protein"
                     /protein_id="HEY5792893.1"
                     /translation="MDTASQSDSSKGASAGTSNIRASVFENPHPPTENFFSRDSAIWR
                     ITREQVLLLGGPAAAILQIAHPAVALGVANHSDFRADTLGRLRRTLDAVYTITFAPHA
                     EVEAMAERVRAAHVRVRGASPQRYSAFSPDAQMWVLTTLIQLSVEMFERFVAPLTPVE
                     KNAFLRDMRIFGVWFGLPAGHGPQGWTEFSAYYHDMLASEIMCSLPISTELAHHIVYP
                     RKPAGLRVLWPLASFTAREFLPSPIREKLLLPRTPGSRLTAATLDALLPRLLPGLPPT
                     LRFAPRYLQASDLQIHPGAGMGIVENG"
     gene            1730..2056
                     /locus_tag="VIS74_06310"
     CDS             1730..2056
                     /locus_tag="VIS74_06310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792894.1"
                     /translation="MAFAHRDGKVCYFNGHLPVFIHESKDLATFRLFSSQLVINGNAT
                     QAQISRAFGVPLVTPANTLTVRLHHLSANVHDEVITHLCNQLTATEAIFPRASLRLIF
                     QFVGSS"
     gene            2171..>2650
                     /locus_tag="VIS74_06315"
     CDS             2171..>2650
                     /locus_tag="VIS74_06315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792895.1"
                     /translation="MKPDSPNCVIREVSKILGEEPMLEAVALSAPEKKLSIATLGGDR
                     GDRLAGRVSQAVSEGSVHCGELAADGRCVACGEKPSGQVLGSRVVVKSVLGNTLIEKQ
                     TCSTAISFWKWISLKWPQYAPREHKPFDGGEEEWRTMAWLAAGCLTFGLLGFAAERLL
                     "
BASE COUNT          497 a          935 c          711 g          507 t
ORIGIN      
        1 gaggcgcgga actcatcgcg cgcggcgaga cggtctgggt ctttgtggac gcggccaccg
       61 ggaaaccccg cctgattccc gcggaagtca gccgcgtctt tacccttgtc ccgcccgcag
      121 aggaacctcc tggcccacaa gccgcagttc cgcccactcg tgctggcggt ggtagttgac
      181 ttcgcggtgc atagacgttg aagaaagcac gggaggcgtg tccgcatccc gatagtcgta
      241 tcggctgaac gacgcccgca gcgccctgcc tttgattgcg ctcacgcaag ccagcacctc
      301 ccagcaatcg ggtgccgtcc gcaccttcaa gcaaaataac ggctcgtcca ccatcaactc
      361 ctccacctcc agatgcccgt tgcgcaggtg ctcgatcacc tgatccgatg caaagcacag
      421 ttgcagccga tgcccggtcg gagccgcgtg cagttccaaa tattcctccc ccgccatatc
      481 ccgcacgaaa atttcaaaca cgtcgcccaa cagccacagc ttctcattgt ccctggtggc
      541 ttgcgtgaag atgcactcat cctccatccg cgcgagcgtc cacaacccgt cggcgttcca
      601 ccccacccgc acctcgccgc cgcggaattc cggcgcggga ttttccaacc aggcttggcg
      661 aaagggcaga acgggcgcat gtttgaaagc cgtggatacg gcatcccaat cggactcttc
      721 aaagggcgcg agcgcgggca catcaaacat ccgcgcatcc gtattcgaaa atccgcatcc
      781 gccaacggaa aattttttct cacgcgactc cgccatctgg cggatcaccc gcgaacaggt
      841 tctcctgctg ggcggacctg ctgccgccat cctccagatc gcccacccgg ccgtggccct
      901 cggcgtggcg aatcacagcg atttccgcgc cgacaccctc ggccgcctgc gccgcacact
      961 cgatgcggtt tacaccatca ccttcgcccc gcacgccgaa gtcgaggcca tggcggaacg
     1021 cgtccgcgcc gcccacgtcc gggtccgcgg tgcctccccg cagaggtact ccgctttctc
     1081 acccgatgcg caaatgtggg tgctcaccac gctcatccag ctcagcgtcg agatgttcga
     1141 gcgtttcgtc gccccgctca ccccggtgga gaaaaacgcc ttcctgcgcg acatgcggat
     1201 cttcggcgtt tggttcggac tccccgccgg ccatggcccg caaggctgga cggaattttc
     1261 cgcctactac cacgacatgc tcgccagcga gatcatgtgc agcctcccga taagcaccga
     1321 actcgcccac cacatcgtct atccgcgaaa acccgccggc ctgcgtgtgc tctggccgct
     1381 cgccagcttc accgcgcgcg aatttcttcc ctcgcccatc cgcgaaaaac tcctcctccc
     1441 ccgcactccc ggcagccgcc tcactgctgc cactctcgac gccctgctgc cccgcctgct
     1501 gcccggcctc ccgccaacgc tgcgcttcgc cccgcgctat ctccaagcct cagacctcca
     1561 gatccaccct ggggccggaa tgggcatcgt ggaaaatgga tgagactgaa agtatgagac
     1621 aggcagggtt ggggagtgat atttttcggt atttttggca gggttggagg ctatgccaca
     1681 agttcagctt ccgatatttc cagatggggt gacgcacatc acgccggatt tggcctttgc
     1741 gcaccgggat ggcaaagtat gttacttcaa tgggcatctg ccggtgttca tccacgaaag
     1801 caaggatctg gccacgttcc ggctctttag cagccagtta gtgatcaatg gcaacgcgac
     1861 gcaagcgcag atttcgcgtg ccttcggagt gccgttggtg acccccgcaa acacgctcac
     1921 cgtccgcctc catcatcttt cggcgaacgt tcacgatgaa gtcatcaccc acctctgcaa
     1981 tcaactcacc gccaccgagg ccattttccc gcgcgccagt ctccgcctca ttttccaatt
     2041 tgtagggtca tcctaattcc tggaggtcag gaggtctgag actggcgctg catttgagcc
     2101 tccatctcaa ttgaccctcc ggcgcgttcc cgggttgttc gccaccgcgg aaagcttagg
     2161 cttgggaaac gtgaagcccg attcgccaaa ctgcgtcatc cgcgaggttt cgaagatcct
     2221 cggcgaggag ccgatgctgg aagccgtcgc cctcagcgcg cccgagaaaa aactctccat
     2281 cgccaccctt ggcggagatc gcggcgaccg cctcgccgga cgcgtttcgc aagccgtgag
     2341 cgaaggcagc gtccattgcg gggaactcgc cgccgacggc cgctgcgtgg cctgtggaga
     2401 aaaaccgtcc ggccaagtgc tgggctcccg cgtggtggtg aaatccgtcc tcggcaacac
     2461 gctcatcgaa aaacaaacct gttccaccgc aatttcgttc tggaaatgga tctccctcaa
     2521 atggcctcaa tacgccccgc gcgaacacaa acctttcgat ggcggagagg aagaatggcg
     2581 caccatggcc tggctcgcgg ctggctgcct caccttcggc ctgctgggct tcgccgccga
     2641 gcgtcttctt
//
LOCUS       DATDAY010000317         3715 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_864146, whole genome shotgun sequence.
ACCESSION   DATDAY010000317 DATDAY010000000
VERSION     DATDAY010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 3715)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3715)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3715
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_864146"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1315
                     /locus_tag="VIS74_06320"
     CDS             <1..1315
                     /locus_tag="VIS74_06320"
                     /inference="COORDINATES: protein motif:HMM:NF019869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEY5792896.1"
                     /translation="PTLKCQVDSPGEGWEQDPDVLDTWFSSWLWAHDTMDDATRAKFY
                     PTSVLVTGPDIIFFWVARMIMAGLEYTGEVPFRHVYFTGIIRDKAGKKMSKSLGNSPD
                     PLDLIAKYGADGIRFGLLRIAPQGQDIKFDEQQIVEGRNFCNKLWNACRFRQMQGPLD
                     ANADPARHSLSLFAQAVLVRLDQTITRVDEAYAAYRFNDVAQALYDFVWGDFCDQFIE
                     VAKSDFYGTDTSRRAGTLATIDFVLKRVLQLLHPYAPFITEELWHGLGFGETTIQFEP
                     WPQAAGLGHAEALKQAAAIHQAVTQSRNLRAIYNLPSNRRLPWHLEPSADWVRGELAP
                     LQTLLNGESLSTEPAPPGSAVAVTEIGKIFLPLAGLIDPEAERERLKKEVLKVESEIE
                     KVAKKLSSETFVNNAPPEIVADHRQRQVDWQARLAMLLKAIEGLG"
     gene            complement(1389..2129)
                     /locus_tag="VIS74_06325"
     CDS             complement(1389..2129)
                     /locus_tag="VIS74_06325"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEY5792897.1"
                     /translation="MTIREEFADLETANGPMRVHLFIPVGEGKYPAVIFYSEIFQVTG
                     PIRRMAAALSGQGYLVAVPEVYHEYEPLGTVLAYDQAGSDRGNELKYAKPIAAYDHDA
                     EVAVKFLQTHANCAGRIGAYGVCLGGHLAVRAGFHPDIAAAAAFYPTDIHTGTLGAGK
                     CDDTLARFAGVKTPFFFAFGRQDPHVPLAGRMKIAECLNAAGADFEWHEFNAAHAFLR
                     DEGARYNPALARVCTELLLRFFAEKLAL"
     gene            complement(2126..>3715)
                     /locus_tag="VIS74_06330"
     CDS             complement(2126..>3715)
                     /locus_tag="VIS74_06330"
                     /inference="COORDINATES: protein motif:HMM:NF013045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEY5792898.1"
                     /translation="GLENLPVKFSGSESQPFRRMLVRIKREIISFGVEGIDPAAHPSP
                     KLAPRELKQWLDEGRPVTLLDTRNDYEIKLGTFKNARPAGIGHFRDFPRAVRQLPPEM
                     KERPIVMFCTGGIRCEKAGPFMEREGFKNIFQLEGGILKYFEECGGEHYEGECFVFDQ
                     RVGVDPALRETETAQCFVCQTPLDAGDQADFRYAPGESCPYCYRTPAEKMGDCLIRRQ
                     VRLREVSAPLPGSVPYDNLRPVNVPASYAGCALLDFLCGFFAHVPREEWREVCARGQY
                     VNDAGETLAADHVVRAGERCLRVMPATVEPDVNAGIRFLYEDEAILVLDKPAPLPMHP
                     SGRFNRNTLQYLLRQVYAPQSPHPAHRLDANTTGLVVCARTRHFAKFLQPQFARGEVA
                     KTYLARVHGHPAADTFASEAPISAGPGELGSREVDEIGGRPARTEFTVRSRNPDGTSL
                     LEVRPLTGRTNQIRVHLWQLGFPIVGDPAYLENGTLGEVMTLAPGDPPLRLHAWRLAF
                     RHPLTNEPVSFETEKPEWSKP"
BASE COUNT          699 a         1200 c         1145 g          671 t
ORIGIN      
        1 ccccacactc aagtgccagg tggacagccc cggcgaggga tgggaacagg atcccgacgt
       61 gctcgacacc tggttttcct cctggctctg ggcgcatgac acgatggacg acgccacgcg
      121 cgcgaagttt tatccgacga gcgtgctggt caccggaccg gacatcattt tcttctgggt
      181 cgcccgcatg atcatggccg ggctggaata caccggcgag gttccgttcc ggcacgtcta
      241 tttcaccggc atcatccgcg acaaggcggg caagaagatg tcgaagtcgc tgggcaattc
      301 gcccgatccg ctcgacctca tcgccaaata cggagcggac ggtattcgct tcggcctgct
      361 gcgcatcgcg ccgcaggggc aggacatcaa gttcgacgaa cagcagatcg tcgaaggccg
      421 caatttctgc aacaagctgt ggaatgcctg ccgcttccgg caaatgcagg gaccgctcga
      481 tgcgaacgcc gacccggcgc ggcattcgct ttcgctcttc gcccaggccg ttctggtgcg
      541 gcttgatcaa acgatcaccc gtgtggacga ggcctacgcc gcttaccggt tcaatgacgt
      601 cgcgcaggca ttgtatgact ttgtctgggg cgatttttgc gatcaattca tcgaagtcgc
      661 caagtccgac ttctacggaa ccgatacctc gcgccgcgcg ggaacgctgg ccacgattga
      721 ttttgtcctc aagcgcgtgc tgcaactgct gcatccctac gcgcccttca tcaccgagga
      781 attgtggcac ggactcggct tcggcgagac gacgattcaa ttcgaaccct ggccgcaggc
      841 cgcgggactg ggtcatgcgg aagcgctcaa gcaggccgcc gcgattcatc aggcggtcac
      901 gcaatcccgc aacctgcgcg ccatctacaa tcttccctcg aaccgccggc tgccatggca
      961 cctcgaacct tcggcggact gggtgcgcgg cgagctcgcg ccgctgcaaa cgctgctcaa
     1021 tggcgaatcg ctcagcaccg aacccgcgcc gccgggctcg gcggtcgccg tcaccgagat
     1081 cggcaaaatc ttcctgccgc tggcgggctt gatcgatccg gaggccgagc gggaacgcct
     1141 gaaaaaggaa gtgctgaagg tggagtcgga aatcgaaaag gtggccaaaa agctttccag
     1201 cgaaaccttc gtcaacaatg cgccgcccga gatcgtcgcc gaccaccgcc agcggcaagt
     1261 cgattggcag gcccggctgg ccatgctgct gaaagcgatc gagggactgg gataattttc
     1321 caaaatcatt gtagaggcgg ttgtgccaac cgcctgtgga tgacgctggg cggctggcac
     1381 agccacccct acaacgccag tttttccgcg aaaaaccgca gcaggagctc cgtgcaaacc
     1441 cgcgccaggg cgggattgta acgcgcgcct tcgtcccgca ggaacgcgtg cgcggcgtta
     1501 aattcgtgcc attcaaaatc cgcgccggcg gcgttgaggc actcggcgat tttcattcgt
     1561 cccgccagcg gcacgtgcgg atcctgccgt ccaaaggcaa agaaaaacgg cgtcttcacg
     1621 ccggcaaacc gcgccagcgt gtcatcgcat tttcccgcgc cgagcgtgcc ggtgtgaatg
     1681 tccgtgggat aaaacgccgc cgccgcggcg atgtctggat gaaatcccgc gcgcacggca
     1741 agatgcccgc ccaggcacac gccataagcg ccgattcggc cggcgcaatt cgcgtgggtt
     1801 tgcaaaaact tcaccgccac ctcagcgtcg tgatcatacg ccgcgatcgg cttggcgtat
     1861 ttcaattcat tgccgcggtc cgatccggcc tggtcatacg cgagcaccgt gcccagcggc
     1921 tcgtattcgt gatacacctc cggcacggcc accagataac cctgcccgct caatgcggcc
     1981 gccatgcggc ggatcggacc ggtcacctgg aaaatctcgg aataaaaaat cacggcggga
     2041 tatttcccct cgcccacggg aatgaacaaa tgcacgcgca tcggtccgtt ggcggtttcc
     2101 aggtcggcaa attcttcgcg aattgtcatg gcttgctcca ttcgggtttt tcagtttcaa
     2161 aggacacggg ttcattcgtc aagggatggc gaaacgccag ccgccaggcg tgcaggcgga
     2221 gcggcggatc gcccggggcc agcgtcatca cttcgcccag cgttccattt tcgagatacg
     2281 ccgggtcgcc cacgatcgga aacccgagct gccacaagtg gacgcggatt tgattcgtcc
     2341 gaccggtcag tggccgaact tccagcaggc tggtgccatc cggattcctc gaccgcacgg
     2401 tgaattccgt ccgcgccggc cggccgccga tttcgtccac ttcccgcgaa cccagctcgc
     2461 cgggaccggc gctgatcggc gcttcgctgg caaacgtgtc cgcggcggga tgtccgtgca
     2521 cccgcgccag ataggttttc gccacctcgc cgcgggcgaa ctgcggttgc aaaaatttcg
     2581 cgaaatggcg ggtgcgcgcg cagaccacga ggccggtcgt gttggcgtcc agccggtgcg
     2641 cgggatgcgg cgactgcggc gcgtaaacct gccggagcaa atattgcagc gtatttcgat
     2701 tgaaccgccc gctcgggtgc atcggcagcg gcgcgggttt gtccaaaaca aggatcgcct
     2761 cgtcctcgta aagaaaccgg atgccggcat ttacgtccgg ctccacggtg gccggcatca
     2821 cccgcaggca gcgctccccg gcccgcacga cgtgatcggc ggccagagtt tcgccggcgt
     2881 cgttgacgta ttggccgcgc gcgcagacct cccgccattc ctcgcgggga acgtgcgcga
     2941 aaaatccgca gagaaaatcg agcagcgcgc agcccgcgta gctggcgggc acgttcaccg
     3001 gccggagatt gtcgtagggc acgctgccgg gcagcggcgc gctgacttcg cgcagccgga
     3061 cctggcggcg gatcaggcaa tcgcccattt tttccgccgg ggtgcgatag caatacgggc
     3121 atgactcacc cggcgcgtag cggaaatccg cctggtcgcc ggcatccagc ggcgtctggc
     3181 agacaaagca ctgcgcggtt tccgtctcgc gcagggccgg atccacgccc acgcgctgat
     3241 cgaacacaaa acattcgccc tcgtaatgct cgccgccgca ttcctcgaaa tatttgagga
     3301 tgccgccctc aagctggaaa atatttttga agccctcccg ctccatgaac ggcccggcct
     3361 tctcgcagcg gatgccgccg gtgcagaaca tcacgatcgg ccgctccttc atttccggcg
     3421 gcagctggcg gacggcgcgg ggaaaatcgc ggaagtgccc gatgcccgcg ggccgcgcgt
     3481 ttttgaaggt gccgagcttg atctcgtaat cgttgcgggt gtcgagcagg gtgacggggc
     3541 ggccttcgtc gagccactgc ttgagttcac gcggagcgag tttgggggat ggatgcgcgg
     3601 cgggatcgat gccttccacg ccgaaggaga tgatctcccg ctttatcctg accagcatcc
     3661 ggcggaacgg ctgggactca ctcccgctga attttacggg gaggttttcc aagcc
//
LOCUS       DATDAY010000318         2515 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_2241086, whole genome shotgun sequence.
ACCESSION   DATDAY010000318 DATDAY010000000
VERSION     DATDAY010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2515)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2515)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2515
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2241086"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            157..1341
                     /locus_tag="VIS74_06335"
     CDS             157..1341
                     /locus_tag="VIS74_06335"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HEY5792899.1"
                     /translation="MRWHEDVGIVAAKGVEVQSIRIDGFTCHPRAIRLPDGEFLLFVT
                     AGPRHYGWAKKEMKGNEVFLYRSQDGWNWSAPEQPWSVPYSQHAPVPFRPSDSDELLA
                     FGTEPEPNHYDGEENAAIGFRRSQDGGRSWSEVRLIAPRNAVDFLGMAAMRLCETADG
                     SWLLGSHVGRWSGIEGVDRKVVTRQFLLRSEDRGASWDLLPGPWPQGWQTPGTERMDE
                     GRPIHLGENRVLFLLRTPTGFLWEMRSADAGRTWTDPQPTTLRHPDAPPMIFHLDEKT
                     LAVFHHNRAVSGAFAHESRTELWLSISQDEGRTWSEPRFLMANACQPAIFDGWGGFTS
                     MISYVDLLVEGDWLHLFVDHQMRQVVQASFHRGAIGFLPDRNQLMGAWSAVGEIAFMP
                     AC"
     gene            1418..2074
                     /locus_tag="VIS74_06340"
     CDS             1418..2074
                     /locus_tag="VIS74_06340"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEY5792900.1"
                     /translation="MVKNPANSRSLAEKAYEHILDRLLKREWLPGQILNRREVAQQLK
                     VSIAPVLEAMVRLEAEGLLTSLPRKGTQVRLVRPEETADHMTVREAIECQAARYYCGN
                     PVLKDEKNLRQLAEAIDNADENSIQIWKKEIAFHGRLVELSGSDALLLAFRQAIHLGT
                     LCTVNLFADLHPNNPEGNHVSLVERLKTKDPDAAARYIREHIRGGKQQLFVGANGFTG
                     "
     gene            complement(2131..2478)
                     /locus_tag="VIS74_06345"
     CDS             complement(2131..2478)
                     /locus_tag="VIS74_06345"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEY5792901.1"
                     /translation="MLFGRDEMLLASLALGAEGWIGSTFNFSGAIYQNLIKAFHSGNL
                     KEARQCQREANEVIRTVAKLSWLPSAKAAFGIYGVDLGTVRPPLKPLSELEVSQLHQQ
                     LKSFPIGLESVVA"
BASE COUNT          518 a          653 c          761 g          583 t
ORIGIN      
        1 aaataccaga tgaaatgtca tactttattc tgattcccac ttagttcgat gtctgcctcc
       61 agttccctcc agccttcagc cgcgcgtaat ctcttgcctt taacggaggt tcggattgcg
      121 gataaaatat ttccttttgg agcgggaggc gattgtttgc gatggcatga agacgtgggc
      181 atcgttgccg cgaaaggggt ggaggttcag agcattcgga tagacggttt tacttgtcat
      241 ccccgcgcca ttcgtttgcc ggatggagag tttctcctgt ttgtgacggc aggcccccgg
      301 cattacggct gggcgaaaaa agaaatgaaa ggcaacgaag ttttccttta tcgctcccag
      361 gatggctgga actggagcgc gcccgagcag ccctggagcg tgccttacag ccagcacgca
      421 ccggttccct ttcggccatc ggactcggac gagcttttgg ctttcggcac tgaaccggaa
      481 ccaaaccatt acgacggcga ggaaaacgcc gcgatcggtt ttcgacgttc tcaggacggc
      541 gggcggtcgt ggtcggaagt aaggctgatc gcgccgcgca atgcggtaga ttttctcgga
      601 atggccgcca tgcggctctg cgaaacggcg gatgggtcat ggcttttggg cagccatgtc
      661 gggcgctggt ccgggatcga gggggttgat cgcaaggtgg ttacccggca attccttctc
      721 cgttcggagg atcgaggcgc gtcctgggat ttattgcccg gtccgtggcc gcaaggctgg
      781 cagacaccgg gaacggaacg aatggacgaa gggcgcccga ttcatctcgg ggaaaatcgg
      841 gtcttgtttt tgctgcgcac cccgacgggc tttctctggg agatgcgcag cgcggatgcg
      901 ggccgcacct ggaccgatcc gcaaccgacg actttgcggc atccggatgc tccgccgatg
      961 atttttcatt tggatgaaaa aacgctggcc gtgtttcacc acaaccgcgc ggtctcggga
     1021 gcgttcgctc atgaaagccg cacggagctc tggctttcca tttctcagga tgaggggcga
     1081 acctggagcg aaccgcggtt cctgatggcc aatgcctgcc aaccggccat tttcgacgga
     1141 tggggtggtt tcacgtcgat gatttcctat gtcgatctgc tcgtcgaggg ggattggctc
     1201 catctttttg tcgatcacca aatgagacaa gtggtgcagg caagctttca ccgcggcgcc
     1261 atcggttttc tgcctgatcg gaatcagtta atgggagcct ggtctgcggt tggggagatc
     1321 gcatttatgc cggcttgttg attgcgcgaa atattacggt gtaggcttat tgaaatagca
     1381 ggcttgattt tatcgcctct ttccgtcttt ttcgggtatg gtaaagaacc cagctaattc
     1441 gcgatcactt gcggaaaaag cttacgaaca catcctggat cgtcttctta agcgagagtg
     1501 gctgccgggg cagattctca atcgccgcga ggtggcccaa caattgaaag tgagcatcgc
     1561 gcccgtgttg gaggcgatgg tgcggctgga agcggaggga ttgctgacct cgctgccccg
     1621 aaaaggcacc caagtgcgct tggtgcggcc ggaagagacg gccgatcata tgaccgtgcg
     1681 cgaagccatc gagtgtcagg cggctcgcta ttattgcggg aatccagttt tgaaagatga
     1741 aaaaaatctc cggcaacttg ccgaggccat cgacaacgcg gatgaaaatt cgattcaaat
     1801 ttggaaaaag gaaatcgcct ttcatgggcg cctcgtggaa ctttccggtt ccgatgccct
     1861 tttgctcgct ttccggcagg ccattcattt gggaacgctt tgcaccgtga atctgttcgc
     1921 cgacctgcat ccgaataatc ccgagggaaa ccatgtctct ctcgtggagc gtttgaaaac
     1981 caaggatccc gatgcggcgg cccgatacat tcgagagcac atccggggtg gcaagcagca
     2041 gctttttgtg ggcgcaaacg gcttcaccgg ctaaggtgag ggagcggctg gttcgtttgg
     2101 ctgcgtcagc tgcccggctt ttcccgccgc ctaagcaacg acggattcga ggccgatggg
     2161 gaagctcttg agttgctggt gcaattgaga gacttccaat tcggaaaggg gtttcaaggg
     2221 cggacggaca gtgccgagat cgacaccgta gatcccaaat gcggccttgg cggagggcag
     2281 ccagctcagt ttcgccacgg ttcggatgac ttcgttagct tctcgttggc attgccgggc
     2341 ttccttgaga ttgcctgaat ggaaggcctt gatgagattt tggtaaattg ctccggaaaa
     2401 attaaaagtg cttccgatcc aaccctcggc tccaagcgca aggctggcaa gcaacatctc
     2461 atcgcggcca aaaagcacgt caaatcggcc gtcctccaga tcgaggcact gctga
//
LOCUS       DATDAY010000319         4112 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_2052530, whole genome shotgun sequence.
ACCESSION   DATDAY010000319 DATDAY010000000
VERSION     DATDAY010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 4112)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4112)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4112
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2052530"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..573
                     /gene="rplE"
                     /locus_tag="VIS74_06350"
     CDS             <1..573
                     /gene="rplE"
                     /locus_tag="VIS74_06350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEY5792902.1"
                     /translation="TEYKTRVVPALRAKHGYTNVHQIPRIQKVVVNTSIGSANDPKEA
                     LEIARNEIATITGQRPISTISKKSIANFKLREAQAIGAKVTLRGRAMYEFLERLIKTA
                     LPRIRDFRGVSTRAFDGNGNYTLGVTDQSIFPEIELDKIKKNIGFDITIVTSARTNEE
                     AKSLLTELGMPFSDRPRLQQAAKEAAAAAN"
     gene            577..975
                     /gene="rpsH"
                     /locus_tag="VIS74_06355"
     CDS             577..975
                     /gene="rpsH"
                     /locus_tag="VIS74_06355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEY5792903.1"
                     /translation="MSAITDPVADFLTRLRNASSVRKTEFTVPYSKLKSEIARLLKQE
                     GYINDYELDTTSDKPFLKITTKFTGKASAITGLKRVSKPGLRRYVGSEEIPRVLGGLG
                     IALVSTSRGILTGQEARRQKVGGELLAYVW"
     gene            998..1537
                     /gene="rplF"
                     /locus_tag="VIS74_06360"
     CDS             998..1537
                     /gene="rplF"
                     /locus_tag="VIS74_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEY5792904.1"
                     /translation="MSRIGNKAITLPDKVKVSVDATGAVNVEGPKGKLAWTLPKDITA
                     KLEGQTLQLNRPDDHRRSKAMHGLARALVQNMITGVNTGFKRDLEIHGVGFRAAVQGK
                     ALNLSLGYSHPVNFEIPTDIKITVTENTKISIEGIDKRLVGQCAADIRGYYPPEPYKG
                     KGIRYTGEQIRRKEGKTVQ"
     gene            1552..1908
                     /gene="rplR"
                     /locus_tag="VIS74_06365"
     CDS             1552..1908
                     /gene="rplR"
                     /locus_tag="VIS74_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015302640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEY5792905.1"
                     /translation="MAATHKRVFRHTRLRKKVSGSEERPRLSVHFSGRHITAQVIDDV
                     SGTTIASAHTTEAEFKSQKGVTANSATAQKVGKLVAERGLAKNVTKVVFDRGGFKYHG
                     KVKALAEAAREAGLQF"
     gene            1921..2487
                     /gene="rpsE"
                     /locus_tag="VIS74_06370"
     CDS             1921..2487
                     /gene="rpsE"
                     /locus_tag="VIS74_06370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEY5792906.1"
                     /translation="MANPNPRDSHRRGPRGPRPESTEPKSENTDKVVFINRCAKVVKG
                     GRRFSFSALIVSGNQKGKIGIGFGKANEVSEAIRKGTDAAHKRMVTVSVAENTIPHEV
                     IGEFGGGRVLLRPASPGTGVIAGGGVRAVVEAAGIRDVLAKSLGSSNPANVVKATLDA
                     LSQLRPREEIFRLRGKALPAKKAAAVAA"
     gene            2573..3070
                     /gene="rplO"
                     /locus_tag="VIS74_06375"
     CDS             2573..3070
                     /gene="rplO"
                     /locus_tag="VIS74_06375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016911111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEY5792907.1"
                     /translation="MRLHDLKPRPGAKHRTKRLGTGESSGHGKTSGKGHKGQKARSGG
                     SIRPSFEGGQMPLFRRLPRRGFNNAEFKTVYGVVNLDDLEEGFEAGVTINEAALREKG
                     LIRGKLDGVKILARGSVTKQFAIEATKVSEAAKEKIEKAGGSVTIIEPKLPVGSRNKA
                     VKAKK"
     gene            3116..>4112
                     /gene="secY"
                     /locus_tag="VIS74_06380"
     CDS             3116..>4112
                     /gene="secY"
                     /locus_tag="VIS74_06380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEY5792908.1"
                     /translation="MISAFRDIFKIPELRQRVIFTLAMIVVVRAGSCITAPGVNAVVL
                     EEWFLHAANSAQGGVAALFNLFSGGSLANCAIFSLGIMPYISASIMMQLLSAVVPQLG
                     KLQREDGGRQKVMQYTRYLTIVLCIFQGYLLALSFENPQSTPFLPNIMATINQLGMPL
                     VDHPGLGFRLLTVITMTAGTMFLVWLGDQITERGIGNGISLIITVGILAQLPAGLVQA
                     WKTFVPSDASAQAAVSPVVLVLMCLFLVIVIAAVIAVTQAQRKISVQYAKRVVGRKVY
                     GGQTQYLPLKVNYAGVMPIIFAQAILLFPSTILQMALPQVKWAQELANQLAVGWLH"
BASE COUNT          894 a         1324 c         1162 g          732 t
ORIGIN      
        1 accgaataca aaacgcgggt cgttccggcc ttgcgcgcga agcacggtta caccaacgtg
       61 caccagatcc cgcgcatcca aaaggtcgtg gtgaacacct cgatcggctc cgcgaacgac
      121 ccgaaggagg cgctcgaaat cgcccgcaac gaaatcgcga ccatcactgg ccagcgtccc
      181 atctcgacca tttccaagaa gagcatcgcg aacttcaagc tgcgcgaagc ccaggcgatc
      241 ggcgcgaagg tgaccctgcg cggccgggcg atgtatgaat ttctcgaacg cctgatcaag
      301 acggcgctcc cgcgcatccg cgacttccgc ggcgtctcca cccgcgcgtt cgacggcaac
      361 ggcaattaca cgctgggcgt gaccgaccaa tccattttcc ccgaaatcga gctcgacaaa
      421 atcaagaaga acatcggttt cgacatcacc atcgtcacct cggcgcgcac caacgaagaa
      481 gccaagtccc tgctcaccga gctgggcatg cccttcagcg accgcccgcg cctgcaacag
      541 gcggcgaaag aagccgccgc cgccgccaat taagccatga gtgccattac tgatcccgtc
      601 gccgactttt tgacccgcct tcgcaacgcc tcgagcgtgc gcaagaccga gttcacggtg
      661 ccttattcca aactcaagag tgaaatcgcc cgcctgctca agcaggaagg ttacatcaac
      721 gactacgagc tggacaccac cagcgacaaa ccctttctta aaatcaccac caaattcacg
      781 ggcaaggcct cggccatcac cgggctcaag cgggtcagca agccgggtct ccgccgttac
      841 gtcggttccg aggaaatccc ccgcgtgctc ggcggactgg gcatcgccct cgtctccacc
      901 tcgcgcggaa ttttgaccgg ccaggaagcc cgccgccaga aggttggcgg agagctcctc
      961 gcctacgttt ggtaatcaat caaccaggaa aaacaccatg tctcgtatcg gaaacaaagc
     1021 aatcacctta cctgacaaag tcaaagtgtc cgtggacgcc acgggtgctg tcaacgtcga
     1081 aggccccaag ggcaaacttg cgtggacatt gccaaaagac attacggcca agcttgaagg
     1141 ccagactttg cagctcaatc gcccggatga ccaccgtcgt tcgaaggcca tgcacggcct
     1201 cgcccgcgcg ctggtccaga acatgatcac cggcgtgaac accgggttca aacgcgatct
     1261 cgaaatccac ggcgtcggct tccgtgccgc cgtccagggc aaggcgctga atctcagcct
     1321 cggctattcg caccccgtca acttcgaaat cccgaccgac atcaagatca cggtcaccga
     1381 aaacaccaag atttccatcg agggaatcga caagcggctc gtcggccagt gcgccgcgga
     1441 catccggggc tactacccgc cggaaccata caagggcaag ggcatccgct acacgggcga
     1501 acagatccgc cgcaaagaag gcaagaccgt ccagtaatag aatcacgacc tatggcagct
     1561 acccacaaac gcgttttccg gcatacccgg ctgcgcaaaa aagtttccgg cagcgaagag
     1621 cgccctcgtt tgagcgtgca cttctccggc cgccacatca ccgctcaggt catcgacgac
     1681 gtctccggca cgaccattgc ctcggcccac acgaccgaag ccgagttcaa gagccagaag
     1741 ggcgtgaccg caaattccgc caccgcccag aaagtcggca agctcgttgc cgagcgcggc
     1801 ctggccaaaa atgtcaccaa ggtggtcttt gaccgcggcg gtttcaaata ccatggcaaa
     1861 gtgaaggctt tggccgaggc cgcccgcgaa gccgggttgc aattctaaga aactctccgt
     1921 atggcaaatc ccaatccaag agacagtcac cgtcgcggcc cccgcggacc gcgcccggaa
     1981 tccaccgaac cgaagtccga aaatacggac aaggtggtat tcatcaaccg ctgcgccaag
     2041 gtggtcaagg gcggacgccg tttcagcttc agcgccctca ttgtctccgg caatcagaag
     2101 ggcaagatcg gcatcggttt cggcaaggcc aacgaggtca gcgaagcgat tcgcaagggg
     2161 accgacgccg cgcacaagcg catggtgacg gtttccgtgg cggaaaacac gattccccat
     2221 gaggtgatcg gtgaattcgg cggaggccgt gttctcctgc gtcccgcttc gccgggaacc
     2281 ggggtcatcg ccggcggcgg cgtgcgtgcc gtggtggaag ccgcgggcat ccgcgacgtg
     2341 ctcgccaagt ccctcggttc cagcaatccg gccaatgtgg tcaaggccac gctggatgcg
     2401 ctcagccagc tccgtccgcg cgaggaaatc ttccgcctcc gcggcaaggc gctcccggcc
     2461 aaaaaagcgg cggccgtcgc ggcctaattg atcagacaga attagccgaa tttacaaaaa
     2521 tgaattcggt taattccgta aattctgtct aaaatcccta cctactattt ttatgagact
     2581 gcatgatttg aaaccccgtc ccggcgcgaa gcatcgcacc aagcgcttgg gtaccggcga
     2641 aagctccggc cacggcaaga cgtcgggcaa aggccacaag ggccagaagg cccgttccgg
     2701 tggttcgatc cgcccgagct ttgaaggcgg ccagatgccg cttttccgcc gcctgccgcg
     2761 ccgcggcttc aacaatgcgg agttcaagac cgtttacggc gtggtcaacc tcgacgatct
     2821 cgaggaaggc tttgaagccg gggtgacgat caacgaggcc gcccttcggg aaaagggcct
     2881 cattcgcgga aaactcgacg gcgtgaagat cctcgcccgc ggttccgtga cgaagcaatt
     2941 tgccatcgaa gcgacgaagg tcagcgaagc cgccaaggaa aaaatcgaaa aggccggcgg
     3001 aagcgtgacg atcatcgagc cgaagctgcc cgtgggaagc cggaataagg cggtcaaagc
     3061 gaagaagtag ccccgctgct tccgagctta agcgccccac ctcagacttc tcccgatgat
     3121 ttccgccttt cgcgacattt ttaaaattcc cgaactccgc cagcgcgtga tttttacgct
     3181 ggcgatgatc gtcgtcgttc gcgccggctc gtgcattacc gcccccggcg tgaatgcggt
     3241 ggtgctcgaa gagtggttcc tccacgcggc caattccgcg cagggcgggg tcgcggcgtt
     3301 gttcaacctc ttcagcggcg gttcgctcgc gaactgcgcc attttctcgc tgggcatcat
     3361 gccttacatc agcgcgtcca ttatgatgca gctgctttcg gccgtggttc cccagctcgg
     3421 caagctgcag cgcgaggacg gcggccggca gaaggtcatg caatacacgc gttatctgac
     3481 gatcgtgctt tgcattttcc aggggtatct cctggcgctt tcgtttgaaa atccgcaatc
     3541 cactccattc ttgccaaaca tcatggccac gatcaaccag ctcggcatgc cgctggtgga
     3601 tcatcccggc ctggggttcc gcttgctgac cgtcatcacc atgacggcgg gaaccatgtt
     3661 cctggtctgg ctcggcgatc aaatcaccga gcgcggcatc ggcaacggca tttcgctgat
     3721 catcacggtc ggcatcctgg cccagctccc ggcgggtttg gtgcaggcgt ggaaaacgtt
     3781 tgtgcccagc gatgcgtccg cccaggcggc ggtcagcccg gtggtgctgg tgttgatgtg
     3841 cctgtttttg gtgatcgtca ttgcggccgt catcgcggtg acgcaggcgc agcgcaagat
     3901 cagcgtccaa tacgccaagc gcgtcgtggg ccgcaaggtc tatggcggcc agacccaata
     3961 cctcccgctc aaggtcaact atgcgggtgt catgccgatc attttcgccc aggcgatcct
     4021 gctttttccg tccacgattc tccagatggc gctgccgcag gtgaaatggg cgcaggaact
     4081 ggcgaaccag ctcgccgtgg gctggctgca tt
//
LOCUS       DATDAY010000320         2771 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_2406865, whole genome shotgun sequence.
ACCESSION   DATDAY010000320 DATDAY010000000
VERSION     DATDAY010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2771)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2771)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2771
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2406865"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2454)
                     /gene="secA"
                     /locus_tag="VIS74_06385"
     CDS             complement(<1..2454)
                     /gene="secA"
                     /locus_tag="VIS74_06385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEY5792909.1"
                     /translation="MINWILKKIVGSKNQRELRKLGPLIRRINELDGALRAESDDALR
                     ARTRAWKEKFSAIRDYDELQKALNDALPEAFAVVKNAARRLEGRPFTVCDQPVTWRMV
                     HFDVQLVGGMCLHQGKISEMATGEGKTLVATLPVYLNALSGRGVHVVTVNDYLAKRDS
                     DWMGQLYRFLGLTIGCIQEDQSPQLRREQYLADITYGTNAQFGFDYLRDNGMATSRDQ
                     QVQRGHYYAIVDEVDSILIDEARTPLIISGPATVSTHQYDKFKPLVDQLVKKQVMLCT
                     RLATEAREAFDAKKPEEAGRLLFKVKLGQPRNKALARFMEDPDLRRLVEKAELEFYKD
                     SEGKQRLVELKEELFFTIDERQHDADLTQKGRDFLNPNDPDAFVLPDLLTEYADIDND
                     AALPPAEKAKKKTDRQAHCDAQAERIHNISQLVKAYCLYEKDVEYVVEENKVIIVDTF
                     TGRKMAGRRWSDGLHQAVEAKEGVQIDRETQTLATITIQNYFRLYDKLGGMTGTAETE
                     ANEFHDIYRLDVSVIPTNRPIQRVDLNDRIYKTRREKYTAVIQEIQAANAKGQPVLVG
                     TASVESSELLSRMLKLQKIPHTVLNAKYHMQEAEIVARAGLKGSVTISTNMAGRGTDI
                     KLGEGVAEAGGLYVIGTDRHESRRIDRQLRGRCARQGDPGVSRFYVSFEDDLMRNFGA
                     SERMTKIMETFGMKEGEELEHPWLNRSVETAQKRVEQRNYLMRRRTLEFDDVMNQQRE
                     VVYGYRNEVMDSAEPRTMVYEVINEVLPKKVASFLETSEEQAEPDYAGLINWLNTTFP
                     LGLTPEKASLDTRTPAENAQ"
     gene            complement(2581..>2771)
                     /locus_tag="VIS74_06390"
     CDS             complement(2581..>2771)
                     /locus_tag="VIS74_06390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792910.1"
                     /translation="SPALRLLIPGFDEDGRVAFWSRQMSEHRLEDNLLVFEGFGNDLT
                     QQWPRIAREIHPHGAERR"
BASE COUNT          455 a          867 c          845 g          604 t
ORIGIN      
        1 ttgggcgttt tccgccggcg tccgcgtgtc gagcgaagcc ttttcaggcg tgagtccgag
       61 cgggaaggtg gtgttcagcc aattgatcaa gccggcgtaa tccggctccg cctgctcctc
      121 gctggtttcg aggaaggagg ccaccttctt cggcagcacc tcgttgatta cctcgtaaac
      181 catcgtgcgc ggctcggcgc tgtccatgac ctcgttgcga tagccataca cgacctcgcg
      241 ctgctggttc atgacgtcgt cgaactccag cgtgcggcgg cgcatgaggt agttccgctg
      301 ctcgacgcgc ttctgcgcgg tttccacgct gcggttgagc cagggatgct ccaactcctc
      361 gccttccttc atgccgaagg tttccatgat cttcgtcatg cgctcgctgg cgccgaagtt
      421 gcgcatgagg tcgtcctcga agctgacgta gaaacgcgac acgccgggat cgccctggcg
      481 cgcgcagcgg ccgcgcagct ggcggtcgat gcggcgggac tcgtgccggt cggtgccgat
      541 gacgtaaagc ccgccggctt ccgccacgcc ttcgccgagt ttgatgtcgg tgccccgccc
      601 cgccatgttc gtggaaatgg tgaccgagcc cttcaggccg gcgcgggcga cgatctccgc
      661 ctcctgcatg tggtatttgg cgttgagcac ggtgtgcggg atcttctgca gcttgagcat
      721 gcggctcagc agctcggagg attccacgct ggccgtgccg acgagcaccg gctggccctt
      781 ggcgttcgcc gcctggattt cctggatgac ggccgtatat ttttcccggc gcgtcttgta
      841 gatgcgatca ttcaagtcca cgcgctgaat cgggcgattc gtcgggatca ccgacacgtc
      901 gagacggtaa atgtcgtgaa attcattggc ctcggtttcc gccgttcccg tcatgccgcc
      961 cagcttatcg taaaggcgga aataattttg aatggtgatc gtcgccagcg tctgcgtctc
     1021 gcggtcaatc tgcacgccct ccttggcttc gaccgcctgg tgcaggccat cgctccagcg
     1081 gcggcccgcc attttgcggc cggtgaaggt gtccacgatg atgaccttgt tctcctccac
     1141 cacgtattcg acgtcctttt cgtagaggca ataagccttc acgagctggc tgatattgtg
     1201 aatccgttcc gcctgcgcgt cgcaatgcgc ctggcgatcc gtctttttct tcgccttctc
     1261 ggcgggcggc agcgccgcgt cgttgtcgat gtcggcatac tccgtgagca ggtccggcag
     1321 gacaaaggcg tcgggatcgt tgggattgag aaaatcgcgc cccttctgcg tgaggtcggc
     1381 gtcgtgctgg cgctcatcaa tcgtgaagaa gagttcctcc ttgagttcga ccagccgctg
     1441 cttgccttcg ctgtccttgt agaattcgag ctcggccttt tcgaccagcc ggcgcagatc
     1501 gggatcctcc atgaaacgcg cgagcgcctt gttgcgcggc tggccgagct tcaccttgaa
     1561 aagcaggcgg ccggcttcct cgggcttctt cgcgtcgaag gcctccctcg cctccgtggc
     1621 caggcgcgtg cagagcatca cctgcttttt cacgagctga tccaccagcg gcttgaactt
     1681 gtcgtattga tgcgtggaaa ccgtcgccgg gccgctgatg atgagcggcg tgcgggcctc
     1741 gtcgatgagg atcgagtcca cttcgtccac gatcgcgtaa taatgcccgc gttggacctg
     1801 ctgatcgcgg cttgtcgcca tgccgttgtc gcgcaggtaa tcgaagccga attgcgcgtt
     1861 ggttccgtag gtgatgtcgg cgaggtattg ctcgcgccga agttgcggcg actgatcctc
     1921 ctggatgcag ccaatggtca ggccgaggaa ccggtagagc tgccccatcc aatccgagtc
     1981 gcgtttggcg aggtagtcat tgaccgtcac gacatggacc ccgcggccgc tgagggcgtt
     2041 gaggtaaacc ggcagcgtcg cgacgagggt cttgccttcg ccggtggcca tttcggaaat
     2101 cttgccctgg tgcaggcaca tgccgccgac caactgcacg tcgaagtgaa ccatccgcca
     2161 agtaaccggc tgatcgcaga cggtgaatgg gcgtccttcc agccgtcggg cggcattctt
     2221 gacgacggca aacgcctcgg gcagcgcgtc gttcagcgcc ttctgcaact catcgtagtc
     2281 ccggatggcg ctgaactttt ccttccacgc ccgcgtgcgc gcgcgcagcg cgtcgtcgga
     2341 ttccgcccgc agggccccgt ccagctcgtt gatgcgccga atgagggggc cgagcttgcg
     2401 aagctcgcgc tggttcttgg aaccgactat tttttttaaa atccaattga tcatgcccga
     2461 aaaaggggtg aaccggtcaa tgaaaggcgg attcgggcaa atgtaaagtg cgcgaaagcg
     2521 ctccccactc tttctcagga gcggtgaaat ccttcgccac gcgccccttg cccaaggaat
     2581 tcaccgccgc tcagccccat gaggatgaat ttcccgcgca atccgcggcc actgctgggt
     2641 cagatcattt ccaaacccct cgaaaacgag caggttgtcc tcgagccggt gttcggacat
     2701 ctgccggctc cagaaagcca cccggccgtc ctcatcaaat cccggaatga gcagccgcag
     2761 tgccggcgat t
//
LOCUS       DATDAY010000321         2124 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_993229, whole genome shotgun sequence.
ACCESSION   DATDAY010000321 DATDAY010000000
VERSION     DATDAY010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2124
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_993229"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            134..1126
                     /locus_tag="VIS74_06395"
     CDS             134..1126
                     /locus_tag="VIS74_06395"
                     /inference="COORDINATES: protein motif:HMM:NF008106.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEY5792911.1"
                     /translation="MKKSLLLAALVLSAAATLHAETKILNASYDVTREFYNEYNPLFA
                     QYWKDKTGKDVTIDQSNAGSSKQARAVVDGLQADVVTFNQDTDIQLLADNGLVPAGWK
                     TLLPNDSSPYTSTIVFLVRKGNPKGIKDWNDLVKDGASVIIPNPKTSGNGRYSYLGAW
                     AYALKQAGGNADAAKAFVAKLFKNVPVLETGGRAATNTFVQKGFGDVLLTFESEVLQI
                     SRVFSPDQFEVVYPSLSILAEPPVAVVAGVVDRNGNREVATEYLKYLWSAPGQELVVK
                     NFYRPRSAELLAKNASLFPPIELFSVDKVFGGWKAAQKEHFADGGVFDQIYAVK"
     gene            1189..1971
                     /locus_tag="VIS74_06400"
     CDS             1189..1971
                     /locus_tag="VIS74_06400"
                     /inference="COORDINATES: protein motif:HMM:NF016817.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase/aspartoacylase
                     family protein"
                     /protein_id="HEY5792912.1"
                     /translation="MKTRRDLAALLRPLDELGAKSGNLFPVHIPLTTARGEPALLPRY
                     YFAGPGTQASFIRVGLFAGIHGDEDAGVYAALRFLDEITREPEKVRGYEFFVYPVCNP
                     TGFADGTRWSRSGLDLNREFWRGTGEIEVQLLERQLENLLFDGILALHADDTSEGLYG
                     FVKGHALTRHVLEPALERAEKVLPRNYDKSIDNFAAQAGVIVEGYRGILSAPPHQHPK
                     PFEIVFETPQLAPLDRQIEASLLAIQEILDRFRVLISEAQNI"
BASE COUNT          425 a          627 c          643 g          429 t
ORIGIN      
        1 aatacggaaa ttcggtccgg gtgccgatcg aaaaatcctg atccttcccc gactcccggt
       61 tttcggcttt cgattggagg gaaaccctcc ggtcgaaagc cgtttccgga ctcaaccctc
      121 ttatccaagt ttcatgaaaa aatctctcct gctggccgcg ctggttcttt ccgccgccgc
      181 cacgctccat gccgaaacca aaattctcaa cgcgtcctac gacgtgaccc gggaatttta
      241 caacgaatac aacccgctct tcgcccaata ctggaaggac aaaaccggca aggacgtgac
      301 cattgaccag tccaacgcgg gctcttccaa gcaggcgcgg gcggtcgtcg atggcctgca
      361 ggcggacgtg gtgaccttca atcaggacac ggacattcag ttgctggcgg acaacggcct
      421 cgttcccgcc ggctggaaaa cgcttctgcc gaatgattcg tcgccttaca cctcgacgat
      481 cgtgttcctc gtgcgcaagg gcaatccgaa gggcatcaag gactggaacg atctcgtgaa
      541 ggacggcgcc tcggtcatca ttccgaatcc caaaacctcc ggcaacggcc gttacagcta
      601 cctcggcgcc tgggcgtatg cgttgaagca ggcgggcggc aacgcggacg cggcgaaggc
      661 gtttgtcgcc aaacttttca aaaacgtgcc ggtgctcgaa accggcggcc gcgcggcgac
      721 gaacaccttt gtccaaaaag gctttgggga tgtcctgctg acctttgaga gcgaagtcct
      781 gcagatttcg cgcgtgttca gcccggatca attcgaggtg gtttatccct cgctgagcat
      841 cctggccgaa ccgccggtcg ccgtggttgc cggggtggtg gaccgaaacg gcaaccgcga
      901 ggtcgccacc gaatatctga aatacctctg gtccgctccc ggccaggaat tggtggtgaa
      961 gaatttttac cgcccgcgtt ccgcggagtt gctggcgaag aatgcgagtc tctttccgcc
     1021 catcgagctc ttcagcgtgg acaaggtgtt cggcggctgg aaggctgcgc agaaggagca
     1081 ttttgccgac ggcggagttt ttgaccagat ctacgcggtg aaataatccg cggatttttc
     1141 atgcccgtgc ttcaaccccg gagggatttt gcggtaaact ttagccgaat gaaaacccgg
     1201 cgcgatctgg cggcgctgct ccggcccctg gatgaactcg gcgccaaatc cgggaatctc
     1261 tttccggtgc atatcccgct cacgaccgcc cggggggagc cggcgctgct gccgcgttat
     1321 tatttcgccg gtccgggaac gcaggcttcc tttattcggg tggggctgtt cgccggcatc
     1381 catggcgacg aggacgccgg ggtttacgcg gcgctgcgtt ttctggatga aatcacccgc
     1441 gagccggaaa aggtgcgggg ctacgaattt tttgtgtatc ccgtctgcaa tcccaccggt
     1501 tttgcggatg gcacgcgctg gtcgcgctcc gggctggatt taaaccgcga gttctggcgc
     1561 ggcaccggcg agatcgaagt tcaattgctg gaacggcagc tggagaacct tcttttcgac
     1621 ggcattctcg ccctgcacgc cgacgatacg agcgagggac tttacggatt tgtcaaaggc
     1681 catgcgctga cgcgccatgt gctggaaccc gccctggagc gggcggagaa agtgctgccg
     1741 cgcaactacg acaagagcat cgataatttt gcggcgcagg ccggggtgat tgtcgagggt
     1801 taccggggca ttctgagcgc cccgccgcat cagcatccca aacccttcga gatcgtgttc
     1861 gagactccgc agctggcgcc gctggaccgg cagatcgaag cctcgctgct ggcgatccag
     1921 gaaatcctcg accgcttccg ggtgctgatt tccgaggcgc agaatattta gagccttttc
     1981 ggtagtattt cgcaaaagag atttccgaaa agggatacaa tgtaggggca gccggagcgg
     2041 agcgaagaca gcctgtcccg cggaagcggg acagcccgag gggcgcacga agtgcggcaa
     2101 ttgtgccaac cgccccgcgt aaac
//
LOCUS       DATDAY010000322         2170 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_1525492, whole genome shotgun sequence.
ACCESSION   DATDAY010000322 DATDAY010000000
VERSION     DATDAY010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2170)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2170)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2170
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1525492"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..733
                     /locus_tag="VIS74_06405"
     CDS             <1..733
                     /locus_tag="VIS74_06405"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HEY5792913.1"
                     /translation="PWIISNACASGSNAVGHAAMLIGTGQAKRAICGGYDAISRLVFA
                     GFDCLRAATPDKCRPFDRNRTGLVLGEGAAILILEEWETAERGSAEILAEIAGYGAAN
                     DNHHLTQPDPSGAGPRRSMQAALAVAGQETVDYVNAHGTGTPHNDASEGAAIGQLLPG
                     VPVSSTKGAMGHSLGAAGAIEAVFCILALRGQFLPPNLNFTTPDEAIPVNVAANPARP
                     AALRSALSNSFGFGGSNASLVFRTA"
     gene            730..1467
                     /locus_tag="VIS74_06410"
     CDS             730..1467
                     /locus_tag="VIS74_06410"
                     /inference="COORDINATES: protein motif:HMM:NF025100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase chain length factor"
                     /protein_id="HEY5792914.1"
                     /translation="MSLRVHSTAWITPLGRGADLAGTPNRGVRRNPFSGREFVCYEPR
                     PADYADAARLPRLRRSSAISHLAVSVARAAAGETDASQWGLVFASADGGIVYTRRFFE
                     PVDARNPGAGSPLLFPETVYNAPASHIAAALGITGTVLTVAGDADAGRRALEAAEQLL
                     RGGLCETCLVVAAQEVDWIMCEAQAAWGDRAIPAEGAAALVVSREGGLPLPEHPADVS
                     LLGECRGVSPLWGLIHGLRLTSPASRR"
     gene            1481..>2170
                     /locus_tag="VIS74_06415"
     CDS             1481..>2170
                     /locus_tag="VIS74_06415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792915.1"
                     /translation="MKTEIVPYAGWENNLRFANGQAELIVSLDIGPRILSYRTPEGDN
                     LLKNFPEQLGQAGEDAWMIRGGHRLWIAPEDEKISYHWDNEPVKSEELEDGSILIRSW
                     QQEPYKIRKDLQIRLADETSQVDIWHAATNEGEEPLQLATWALTVFNPNGLAIFPQPP
                     LGEHPRDLLPNRNIVVWPYTDLGDPRLIFGQRFITLQQLPDRPPLKLGLSHQEGWIAY
                     LWGESLFFKTFD"
BASE COUNT          353 a          744 c          726 g          347 t
ORIGIN      
        1 gccgtggatc atttccaacg cctgcgcctc gggctcgaat gccgtggggc acgccgccat
       61 gctgatcgga accggccagg cgaagcgggc gatctgcggg ggctacgacg ccatttcgcg
      121 gcttgtcttc gcgggatttg actgcctccg ggccgcgaca ccggacaaat gccggccgtt
      181 tgaccgcaac cgcaccggcc tcgttctcgg cgagggcgcg gcgatcctga tcctggagga
      241 atgggaaacc gccgagcgcg ggagcgcgga aatcctggcc gagatcgcgg gctacggcgc
      301 ggccaacgac aaccaccacc tcacccagcc cgatccctcc ggcgcgggcc cgcggcgttc
      361 gatgcaggcc gcgctggcgg tcgcgggaca ggaaaccgtg gattacgtca acgcccacgg
      421 caccggcact ccgcacaacg acgccagcga aggcgcggcc atcggccagc tgcttcccgg
      481 cgtgccggtg agcagcacga aaggcgccat ggggcattcc ctcggcgcgg cgggggcgat
      541 cgaggccgtc ttctgcatcc tcgcgctgcg cgggcagttc ctgccgccga acctgaattt
      601 taccacgccg gacgaagcca ttccggtcaa tgtcgcggcc aatcccgccc gcccggcggc
      661 gctgcgcagc gcgctttcca actccttcgg ctttggcggg tccaacgcga gcctggtttt
      721 ccgaacggca tgagcctgcg cgtccattcg acggcctgga tcaccccgct cgggcggggc
      781 gctgacctgg cgggaacgcc aaaccggggg gtgcgccgga atcccttctc cgggcgggaa
      841 ttcgtctgct acgaacccag gcccgcggac tacgcggacg ccgcgcggct gccccggctg
      901 cggcgctcca gcgccatttc ccatctcgcg gtatcggtcg cgcgcgcggc ggcgggcgaa
      961 accgatgcga gccaatgggg gctggtgttt gcctcggcgg atggcggcat cgtttacacc
     1021 cgccggttct tcgagccggt ggacgcgcgg aatcccggcg cgggcagccc gctcctgttt
     1081 ccggaaacgg tttacaacgc tcccgccagc cacattgccg ccgccctggg catcaccggc
     1141 acggtcctga cggtggcggg agacgcggac gccgggcggc gggcgctgga agccgcggaa
     1201 cagctcctgc ggggcggcct ctgtgaaacc tgcctcgtcg tcgcggccca ggaagtggat
     1261 tggatcatgt gcgaggcgca ggcggcgtgg ggggatcgcg ccattcccgc ggaaggcgcc
     1321 gcggcgcttg tggtctcgcg ggagggcggc ttgccgctgc cggagcaccc ggcggatgtg
     1381 tccctgctgg gcgaatgccg gggagtctcg cccttgtggg ggctcattca tggactgcgc
     1441 ttgacttccc ccgccagccg ccgctgaata cattcacttc atgaaaacgg aaatcgtgcc
     1501 ctatgcgggc tgggaaaaca acttgcgctt cgccaacggg caggcggaac tcatcgtgag
     1561 tctcgacatc ggtccccgca ttctcagcta ccgcacgccg gagggcgaca acctgctgaa
     1621 gaactttccc gagcaactcg ggcaggccgg cgaagacgcg tggatgatcc gcggcggcca
     1681 tcgcctctgg attgcgccgg aggacgaaaa aatctcgtat cactgggaca acgagccggt
     1741 caaatccgag gagctggagg atggctcgat cctcatccgc agctggcagc aggagcccta
     1801 caagattcgc aaggacctac agattcgcct ggccgacgaa acctcgcagg tggacatctg
     1861 gcatgcggcc acgaatgagg gcgaagagcc gctacagctg gccacctggg ccctgaccgt
     1921 ctttaatccc aatggactgg ccattttccc ccagcctccg ctgggcgaac atccgcggga
     1981 tttgctgccg aatcgcaaca tcgtggtctg gccctacacc gacctgggcg acccgcggct
     2041 gatcttcggc cagcgcttca tcacgctcca gcagctgccc gaccggccgc cgctcaagct
     2101 ggggctctcg caccaggaag gctggatcgc ctacctgtgg ggcgagtcgc ttttcttcaa
     2161 gaccttcgat
//
LOCUS       DATDAY010000323         4160 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_2055146, whole genome shotgun sequence.
ACCESSION   DATDAY010000323 DATDAY010000000
VERSION     DATDAY010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 4160)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4160)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4160
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2055146"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(219..482)
                     /locus_tag="VIS74_06420"
     CDS             complement(219..482)
                     /locus_tag="VIS74_06420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792916.1"
                     /translation="MVEIEVYAAGLRNPDRIVGLDFELETIAGLRYKVDTNHDIVYME
                     FSGEPLSLHDLKSIFRKLGLDAKFVGALPPEVSSKKKTQRLEP"
     gene            739..4095
                     /locus_tag="VIS74_06425"
     CDS             739..4095
                     /locus_tag="VIS74_06425"
                     /inference="COORDINATES: protein motif:HMM:NF024787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease domain-containing protein"
                     /protein_id="HEY5792917.1"
                     /translation="MKSYPLQTPSATPNCRDLHLAFDVGHSSIGWAVLQVPANDVPNL
                     LGCGAVIFQADDCLASQRRAFRRQRRHIRSTRLRIARMKALLAHLRVFTAEGMDGVQS
                     SSPWFLAARVLAGGDPLTWPELWDVLRWYAHNRGYDGNRRWNASEAEAEQADTEKVEN
                     AHALCQKYGTTSMAATLCAALEIDPLGKKSASMIRFKGLDAAFPREQVEREVIQILQA
                     HFDRLPAVNEALIKSLIEDWRAIPCPHLRLPDRYKGGLLFGQLVPRFENRIIAQCPVT
                     YEREYQRLLAETGDEQKAKRGAAKAAKVPSRDSVEFYRYRWAMQLANVQIATGEGRKT
                     RPLTVEERRTVDAAMREHGSLTVKQFKEAVRQLTGGLPDNLGTMLSHPEAQKALLLDP
                     ALKQTGTGALAIIWPHLDETTRKHTLTKLRRFKRLSVREIVGDHFPALAALENHVIAA
                     NTKKDKKSAPISLDELLAEYRQLSPQGLRAPYHREVMIEACRDVFEKGIHPKEEGGCL
                     YRDERIRAAQLQRAIDEQTNNHLVRHRLLILERLHRDMLKEYAGEDPKRVAKLTIEVN
                     RELREMSGKTAKEVAMEEGQRLANFKNVTKKLEAAFAGKNIVITAGLIRKARIAEDLG
                     WKCPYTGKEYDAFDLLTRKVDKDHIIPRSLRPSDSLDSLVITFSEVNRMKGQRTALQF
                     IEDEQGKVVEGAPQLSLKTAANFWKEVESLETFKGHEDDQRRKKNRKRLLQLREYVEK
                     EFTPGDLTQTSQLVRLGAQTLQRSYLDLDRLPVMTSLPGAVTGGVRKSWRLLGCLTEA
                     NPGVTEETTKTEVRHITHLHHALDASVLAFASHFLPRNGNLWELLVKRRLTEAEQAQL
                     LQLSSQFQRGSDGRVSLSDLPVPLKKQLRERLAERRVVQHIPADPSGLVCDETVYRVF
                     DPADPHPSARQLAQWFRKQNAAIPAKDSDSVLITNRKRRSAAGPESGRLLHETRTWRW
                     TYQLIEKSKLLGLEPRDPATAKLQPLKAVKILSGNFGLALDPEPEVLRAHKIWPKLRE
                     LKAKNGGKWPRILRNGMLIEAPRGKFQGVWKVASAKANLKLDLISADRVKMESSGAGI
                     KGNVLLKSLIKDGLIIVKTSLVGYGNAQRD"
BASE COUNT          960 a         1293 c         1166 g          741 t
ORIGIN      
        1 ataatgacca ccgcctggga gcgatagccg atttcgcaaa cttggcggac caccagccgc
       61 ccgcggatgc gccccgcaaa aatcgaggca aacgcctcgc aagccagcaa cgcaagcccg
      121 cccaaaaact cgacaaaccg ctggcagatc gagccggtga aatgaaagac acccatccga
      181 attacgccag gagagggccg cgccggttcc gcccaatctc agggctcaag ccgctgggtt
      241 ttctttttcg agctgacttc cgggggcagc gctcccacga acttcgcatc cagccccagc
      301 ttgcggaaaa tggatttcag gtcatgcaag ctcagcggct cgccggaaaa ctccatgtaa
      361 acgatgtcgt gattcgtatc caccttgtaa cgaaggcccg caatcgtctc caattcgaaa
      421 tccagcccga caatcctgtc cggattccgc aaccccgccg catagacctc aatctcgacc
      481 atgcccccca gcatcggatg cgccccgcgg aaatcaagac gaaatgaaga tgaactaaat
      541 gggatttacc ggattaacga aaagacaaat cgagaaattg aaaacattcg aaccgcggcg
      601 ggatggaacc agttgtgagg caaataaaaa agcggagaca agctccgctt ttttatttaa
      661 gaaacgcttt tgaaccggtg tgaggcgcct ggcgctcagg gaaacggtct gcgctatcta
      721 actttgtctt gattccaaat gaagagctac cccctccaaa ctccttctgc aaccccaaat
      781 tgccgcgatc ttcacctcgc cttcgacgtc ggccacagct ccatcggctg ggccgtgctg
      841 caagtccccg ccaacgacgt tcccaacctc ctcggctgcg gcgccgtcat tttccaggcc
      901 gacgattgcc tggcctccca gcgccgcgcc ttccgccgcc agcgccggca catccgctcc
      961 acccgtctgc gcatcgcgcg aatgaaagcg ctgctggctc atctcagagt tttcactgcg
     1021 gagggaatgg acggcgttca atcgtcgagt ccgtggtttc tcgccgcccg ggtgctggcc
     1081 gggggcgatc ccctcacctg gccggaactc tgggacgtgt tgcgctggta tgcgcacaat
     1141 cggggatacg atggcaatcg ccgctggaac gcctccgagg ccgaggcgga acaggcggac
     1201 accgagaaag tcgaaaacgc ccacgcgctg tgccagaaat acggcaccac ctcgatggcc
     1261 gccacgcttt gcgccgcgct ggaaatcgac ccgctcggca aaaaatccgc gtccatgatt
     1321 cgtttcaaag gcttggacgc cgcgtttccc cgtgagcagg tcgaacgtga agtcattcag
     1381 attctgcagg cgcacttcga ccggcttccg gcggtaaacg aggcgctgat caagagcttg
     1441 atcgaggatt ggcgggctat cccctgtcct catctgcgtt tgccggatcg ctacaagggc
     1501 gggctgcttt tcgggcaatt ggtgccaagg tttgagaatc ggatcattgc gcaatgtccg
     1561 gtcacttatg aacgcgaata tcagcgtctg ctggcggaaa ccggcgatga gcaaaaagcc
     1621 aaacgagggg cggccaaggc cgccaaggtt ccgtcgcggg actccgtgga gttttaccgc
     1681 tatcgctggg ccatgcagct tgcgaatgtg caaatcgcga ccggtgaagg ccgcaaaacc
     1741 cggccattga ccgtggagga acgccggaca gtggatgcag cgatgcgtga acacggcagc
     1801 ctcaccgtga agcaattcaa agaggcggtt cgccaactga ccggcggact gccggacaac
     1861 cttggcacga tgctctccca ccccgaggcg cagaaggccc tgctgctgga tccggcattg
     1921 aagcaaaccg gaaccggcgc tctggcgatt atctggccgc atctggatga aacgacccgc
     1981 aaacatacgc tcaccaagct gcggcgcttc aagcgcctgt cggtgcggga aatcgttggc
     2041 gaccatttcc ccgccctggc cgctctggaa aatcacgtca ttgccgctaa tacgaaaaag
     2101 gacaaaaagt cggctccaat ttccctcgat gaactgttgg ccgaataccg ccagctttct
     2161 ccccagggat tgcgcgcgcc ctatcatcgg gaagtcatga tcgaagcctg ccgcgatgtc
     2221 tttgaaaagg gcattcatcc caaagaggaa ggcggctgcc tttatcgcga tgagcggatt
     2281 cgcgccgcgc aattacagag ggccattgac gagcagacca acaatcacct ggttcgccat
     2341 cggctgctca ttctggaacg cctgcaccgc gacatgctca aggaatacgc cggcgaggat
     2401 cccaagcggg tggcgaagtt gaccatcgaa gtgaatcggg aattgcggga aatgtccggc
     2461 aagaccgcga aggaagtggc catggaggaa ggtcagcggc tggccaactt caagaatgtc
     2521 accaaaaaac tcgaagcggc tttcgccggg aaaaacatcg tcattaccgc cgggttgatt
     2581 cgcaaagcgc gcatcgccga agacctcggc tggaagtgcc cttacaccgg caaggaatac
     2641 gatgcgttcg atctgctcac ccgcaaggtg gacaaggatc acattatccc gcgcagcctc
     2701 cgaccgagcg acagcctgga ctcgctggtg atcacgtttt ccgaggtcaa ccggatgaaa
     2761 ggccagcgca ccgctctgca attcatcgag gacgaacagg gcaaggtcgt ggagggcgcg
     2821 ccgcaactca gcctgaaaac cgccgcgaac ttttggaagg aggtggaatc gctggagacc
     2881 ttcaaggggc atgaggacga ccagcgccga aagaaaaatc gcaaacgcct gctgcaattg
     2941 cgcgaatacg tcgaaaagga attcaccccc ggcgatctga cccagacctc gcagctcgtc
     3001 cggctgggcg cgcaaacgct gcaaagatcc tacctcgatc tcgacaggct gcccgtgatg
     3061 acgtctcttc cgggcgcggt caccggcggc gtccgcaaga gctggcggct gctcggctgc
     3121 ctcaccgagg caaatcccgg cgtcacggag gaaaccacca agaccgaggt tcgccacatc
     3181 acgcatctcc accatgcgct cgacgccagt gtgctcgcct tcgcctccca cttcctgcct
     3241 cgaaatggca atctctggga gcttcttgtc aaacgccggc tgaccgaagc cgagcaggcg
     3301 cagttgctcc agcttagcag ccagttccag cgcggttcgg atggccgggt ttccctcagc
     3361 gatctgcccg tccccctgaa aaagcagctc cgcgaacgcc tcgccgagcg gcgggtcgtc
     3421 cagcacatcc cggccgaccc ctccggcttg gtctgcgacg aaaccgtcta tcgcgtcttc
     3481 gatccggccg atccgcatcc ctctgcccgg cagctggccc agtggttccg aaaacaaaat
     3541 gccgccatcc cagctaagga cagcgacagt gtgctgatca ccaaccgcaa acgccgctca
     3601 gccgccggcc cggaaagcgg caggctgctc cacgaaacca ggacctggcg ctggacgtat
     3661 caattgatcg aaaaatccaa gcttctcggc ctcgaaccgc gcgacccggc cactgccaag
     3721 ctccagccct tgaaggccgt caaaatcctc agtggtaatt tcggcttagc gctcgatccc
     3781 gagcccgagg tgctccgagc ccacaaaatc tggccaaagc tccgcgaact caaagccaaa
     3841 aacggcggca aatggccacg gattctgcgg aatgggatgt tgatcgaggc gccgaggggc
     3901 aagtttcagg gagtttggaa agtcgcatcg gcaaaagcca atttgaaact cgacttaatt
     3961 tctgccgacc gagtgaagat ggaaagttcc ggcgcgggca tcaaaggcaa tgtgcttctg
     4021 aaaagcttga tcaaagacgg tttaatcatc gtaaagacgt cgttggttgg atatgggaat
     4081 gctcaacgag attgacgtta tccgcgacgt ttccacccgg ctggaccggc atcacatcgc
     4141 ttatatgctg acgggatcga
//
LOCUS       DATDAY010000324         2823 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_2509360, whole genome shotgun sequence.
ACCESSION   DATDAY010000324 DATDAY010000000
VERSION     DATDAY010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2823)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2823)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2823
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2509360"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            38..703
                     /locus_tag="VIS74_06430"
     CDS             38..703
                     /locus_tag="VIS74_06430"
                     /inference="COORDINATES: protein motif:HMM:NF020387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RES family NAD+ phosphorylase"
                     /protein_id="HEY5792918.1"
                     /translation="MIRSPRLPLEQPAFDLLEMPVSEADPQTWVNLRFAEFCDKKHLF
                     YANAGARLTPHSLNIPGVYLARTEKTAFLELYGDRLDAARQAGTQMILAEAELNRRVF
                     AGIRTESLRLCDLARKGGGSKLRLDAGTLRASDLEYPQKFAEAICHHPAEVDGIRYRS
                     RHTDEVCLVVWNRREVARLENTAPAPLRSHLRATDIRSAKLFGEEIWLAGTPQTFDEL
                     TQN"
     gene            complement(763..837)
                     /locus_tag="VIS74_06435"
     tRNA            complement(763..837)
                     /locus_tag="VIS74_06435"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(803..805),aa:Cys,seq:gca)
     gene            complement(856..1557)
                     /locus_tag="VIS74_06440"
     CDS             complement(856..1557)
                     /locus_tag="VIS74_06440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792919.1"
                     /translation="MKRLLPFLLTLAAVDFLGADDKPLFREPGFVYLADFGQKPLRVP
                     LNNGPANAYFDLALTRYAGTLRYPQEVEVLGFGDRLYRIRGRAQQGQILAYVPPDSLA
                     PVDPEFIANLRKAEERRKLVEALIAKNEAALGMTLEEVRLSLGKPQKKSQKANAEGVE
                     QVWEFVTYTLIPQTTTVIGPGGVQTLATTYIKTPKGKMEVHFKNGIVSELNQSEGTVL
                     TGNETTIVAPPIFVY"
     gene            complement(1735..>2823)
                     /locus_tag="VIS74_06445"
     CDS             complement(1735..>2823)
                     /locus_tag="VIS74_06445"
                     /inference="COORDINATES: protein motif:HMM:NF013752.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEY5792920.1"
                     /translation="RENGRLLERAFSHVLELAARVGVMKVGKVTVAIDGTKILANASK
                     HSAVSYEKAGEAMRELDWEVAELLKKAETADSTPLEDGLTVPEEVARREERKAKLQAA
                     RAVMEARAAVRAAAERAEYEAKKAEREARSEQGKKPRGKEPKEPEGKPGAKDQMNFTD
                     PESRIMPCGGKTHFEQAYNAQAGVEIESRLIITKRVSHASNDKQELVEDVRAIDPVIE
                     SVETILIDSGFVSEAAVAEVEAPREDGGSAPKVIGAVQRQSHHRSVRDLEKKEDPPAP
                     GDEAAFTEKMAHRTATKEGREKYKQRQQTIEPVFGIIKEAMGFRRFSLRGQGKAALEW
                     TLVCVSYNLRRLHRLMTAQTPKMTPAWA"
BASE COUNT          472 a          862 c          817 g          672 t
ORIGIN      
        1 ggccgccgct gccgcccgtt attgaggcgc ttggcgcatg atccgatccc ctcggcttcc
       61 cctggagcag ccggcatttg acctcctgga aatgccggtt tccgaggcgg acccgcagac
      121 gtgggttaat ttgcgatttg cggaattttg cgacaaaaag cacctgtttt acgccaatgc
      181 aggcgcgcgg ctgactccgc attcgttaaa cattcccggt gtctatctgg cgcggacgga
      241 gaaaaccgcc tttcttgaac tttacgggga ccggctggat gcggcccggc aggcgggcac
      301 gcaaatgatc ctggcggagg ccgaattgaa ccggcgggtt tttgccggga ttcgaaccga
      361 gtcactccgg ctttgtgatc tcgcccggaa gggcggcggt tcgaaactgc gtctcgacgc
      421 gggaaccctc cgggcttccg acttggagta tcctcaaaaa tttgccgagg ccatctgcca
      481 tcaccctgcc gaagtggatg gcattcgtta tcgaagccgc cataccgatg aggtttgcct
      541 ggtggtttgg aaccgccggg aggtggcgcg tttggaaaat accgctcccg cgccccttcg
      601 ttcccatctg cgggccacgg atatccgcag cgccaagctg tttggcgaag agatctggct
      661 ggccggaacg ccgcagactt tcgacgaatt aacccaaaat taggaagatc ttcttgagct
      721 acagtcgagc tacataattc ttctaactcc ttgaatttaa gatggaggcg agggtcggaa
      781 tcgaaccgac gcatacagct tttgcagagc cgggccttac cacttggcta ccccgccatc
      841 acgaaagaac ggtcactaat acacgaagat cggcggggcg acaatagttg tttcgttccc
      901 cgtcaaaacc gtgccctcgc tctggttgag ctcgctcacg atgccattct tgaagtgcac
      961 ctccatcttg cccttgggcg ttttgatgta ggtggtggcc agcgtttgca cgccgccggg
     1021 accgatgacg gtcgtggttt gcgggatcag cgtgtaggtg acaaattccc agacctgctc
     1081 gacgccctcg gcgttggcct tctggctttt tttctgcggc ttgccgaggc ttagccggac
     1141 ttcctcgagc gtcatgccga gggcggcctc gtttttggcg atcagggcct cgaccaattt
     1201 gcggcgctcc tccgccttcc gcaggttggc gatgaattcg gggtccacgg gcgccagtga
     1261 atcgggcggc acgtaagcca gaatctggcc ctgctgggcg cggccgcgga tgcggtaaag
     1321 gcggtcgccg aaacccagca cctccacttc ctgcgggtaa cgcagcgtgc cggcgtagcg
     1381 ggtgagggcg aggtcgaaat acgcattggc cggcccattg ttcaagggca cgcggagcgg
     1441 tttctgcccg aaatccgcca ggtaaacgaa tcccggctcg cggaacaggg gcttgtcgtc
     1501 ggcgcccagg aaatcgaccg ccgccagggt gaggaggaac ggaaggaggc gcttcatcga
     1561 gatttaatct agcagcctgt cggactgaaa aacgagcggt aaaagccgcg ttttccagat
     1621 ttttacgatc acgtcgtaaa attatgagcg gcaaggtttc ctgaagcgaa cccctggtgg
     1681 attggatggg ttttttgctc ggcccctctc ttctgggagg cgggaatgtc ccggtcaggc
     1741 ccatgccggg gtcatttttg gagtttgggc ggtcatcagg cgatggagac ggcgcaaatt
     1801 gtaggacacg cagacaagcg tccactccaa ggcggctttt ccctggcctc gcaaactgaa
     1861 ccttcggaac cccatggcct ctttgataat gccaaagacg ggttcaatgg tttgctgcct
     1921 ttgtttgtac ttttcccggc cctctttggt ggcggttcga tgggccattt tttcggtgaa
     1981 ggcggcctcg tcgccagggg ctggcgggtc ctccttcttt tccaaatccc tcacgctgcg
     2041 atgatggctt tggcgctgga cggcgccgat cactttggga gccgaccctc cgtcttcccg
     2101 aggagcttcc acttctgcga ccgccgcttc gctcacgaat ccgctatcaa tcaagatggt
     2161 ctctacgctc tcgatcacgg ggtcaatggc tctcacgtcc tcgactaatt cctgtttgtc
     2221 gttggaggca tggctcaccc gcttagtaat gatcaggcgg ctctcaattt ccactcccgc
     2281 ctgcgcgttg taagcctgtt cgaaatgcgt tttccctcca cacggcatga tccggctttc
     2341 cgggtcggta aagttcatct ggtctttggc tccgggcttg ccctctggct ccttgggctc
     2401 tttgccacgg ggcttttttc cctgttcgct ccgggcttcc cgttccgctt ttttcgcctc
     2461 gtattccgca cgttccgccg ccgcccgcac cgccgccctt gcctccatga cggcgcgggc
     2521 cgcctggagt ttggctttgc gttcttctcg ccgcgcgacc tcctccggca cagtcaggcc
     2581 gtcttccagc ggcgtgctgt ctgctgtctc ggcttttttc agtaactcgg cgacttccca
     2641 gtccaactcg cgcatcgctt ccccggcctt ctcgtagctc accgcgctgt gtttgctcgc
     2701 attggccagg atcttcgtcc cgtcgatggc cacggtaact ttgcccacct tcatcactcc
     2761 gactctcgcc gccagttcca agacgtgact gaaggctctt tccagcagtc gcccgttttc
     2821 ccg
//
LOCUS       DATDAY010000325         6711 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_467923, whole genome shotgun sequence.
ACCESSION   DATDAY010000325 DATDAY010000000
VERSION     DATDAY010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 6711)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6711)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6711
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_467923"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(176..2185)
                     /locus_tag="VIS74_06450"
     CDS             complement(176..2185)
                     /locus_tag="VIS74_06450"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HEY5792921.1"
                     /translation="MRYTLETPPAHRRIAYDRELNEQQLAAVTSKPGAALVIAGAGSG
                     KTRTLTYRVAWLLEQGFPPYAILLLTFTNKAAREMLDRVNALLPEMSAGIWGGTFHSI
                     GNRILRRHAERIGFRPGFSILDREDSEDLLEAVVVREGIRANDKRFPKGSVLAELFSL
                     AINTGQPLARIIATRYRYFTPLLEPIERVQAAYEGRKREANSMDFDDLLSKLLELFET
                     HPDVREHYQNQFRQILVDEYQDTNRLQAELIARLAGKHGNLMVVGDDAQSIYSWRGAD
                     CLNILEFPKHYPGARVFKIETNYRSVPEVLELANASITGNLAQFEKALRPVRAEKRVR
                     PGLVKLGTNNQQAAFAAQRVLELHEEGIAFSEMAVLYRSHFHSMEVQLEFTRRGIPYT
                     ITSGLRFFEQAHVKDVAAFLRFAVNPRDEMSFKRFVKLLPGVGGKSAEALWEKSQRIL
                     EGGRDFARLLEGVRPPAKAAEGWKQLVHTLEELVPNGEPAPPAEMIQSVVLAIYDDYM
                     RTQFANYEARREDLQTLASYSKSFASAEEFLSQLALLGALETADATASAGNEERVTLS
                     SIHQAKGLEWQVVFLIWLTEGMFPSSRSVENPESLEEERRLFYVGVTRCKDELYLTYP
                     EMRLNAGYGDVFQRPSRFLEELPRHLLEPWEVESGIRASLPEWGE"
     gene            complement(2189..3031)
                     /locus_tag="VIS74_06455"
     CDS             complement(2189..3031)
                     /locus_tag="VIS74_06455"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HEY5792922.1"
                     /translation="MRIVALSLALALLACRVEAAEPPVITAKAALLFDATSGEVLLKK
                     NENAQRPVASTQKLLTALIIAEAGGLDQLVTVEQSDTLADPTKLYLKTGDQYTRQELL
                     SALLVKSANDVACALARDNAGSVEAFARKMNARIAELRGTNSHFINPNGLPVEGQYST
                     ARDMAIVARAVYANPVLRDIISQKFITFRYASGKTVTLRNTNRLLQEYSFCNGMKTGY
                     TIAAGHCLITSASWGGRQVIAVVLGDTKLRVRKESVDLLAYGLGISKDGVASMRVSSG
                     GSAN"
     gene            complement(3043..3690)
                     /locus_tag="VIS74_06460"
     CDS             complement(3043..3690)
                     /locus_tag="VIS74_06460"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY5792923.1"
                     /translation="MRTGLLILIALAGMAGTLFAQEEYLPDSAKLAISQGNEAFADGD
                     YRKAREAYETVLKISPRYLLAQVNLGMTQFYLGDRGAAEKLLKQAVQQRIETAPAWLI
                     LGLIYLDQNKLDAALAALSQATLYDPKNARAHNYLGVVVGRLGWLDAAEAELRKAIEL
                     DEKYADANYNLALFYLDRKKPAVELARRHYLRALELGAEKDAEIEKMLSNSAILR"
     gene            complement(3692..4519)
                     /gene="proC"
                     /locus_tag="VIS74_06465"
     CDS             complement(3692..4519)
                     /gene="proC"
                     /locus_tag="VIS74_06465"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEY5792924.1"
                     /translation="MSVPSGLKFSFVGAGRMNRALVQGLLRVGVAAPGDIHVSTRTPE
                     KLAALREEFGIVTAPDNASAAGAGDVVIFGVKPADAPAAIESCKSALKAKLVVSVMSG
                     LAISTLREMTGASPRLVRAMPNVAALAGAGATAVAFDAKHTEVDVHAAELLFGSVGKV
                     YPVKESLLDAVTGLSGSGPAYLYLVMEALAEGGVKAGLPRDLAQQLAVQTVLGSAQLA
                     VETKQHPALLREMVTSPGGTTAAALAVLEDRGVRGAFIAAVEASARRAAELGSVAKK"
     gene            complement(4532..5524)
                     /locus_tag="VIS74_06470"
     CDS             complement(4532..5524)
                     /locus_tag="VIS74_06470"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEY5792925.1"
                     /translation="MIAFGLLLLALSPLRIDSAADYSRRHGGEVLVIREAGRTVHDEQ
                     SMAARRGERYACLSITKSLVGTLFLAARNDGLLDLDERASDTLTEWRKKPAKRDIRIR
                     DLLNLQAGLSPGYHCLYRKKIANKAKEAVALGMTGEPGGQFAYGPADFEVLEELLARK
                     LKARGVSPLSYFQRRLFNYIGASIEGWRLDLSGNPYFSTGARLSVPQFLRFGDIVLNA
                     GRPGLTRWFCADLGAIYDPRAGLPVYALGFWRNLSRGNEINVEREIGERTELSFWRDA
                     CLSRSAPRDMVAMIGSYGQRLYVVPSRKLVVLRLGQSRHFDDAAFLRELFGPAR"
     gene            complement(5521..>6711)
                     /locus_tag="VIS74_06475"
     CDS             complement(5521..>6711)
                     /locus_tag="VIS74_06475"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY5792926.1"
                     /translation="RQAGKAEENEAGAMSVILHETLAGIRVIKSFARENYQADQFAKS
                     SQEQFRISLKVRKSSDIVQPLIETVSALGIALALLYVFFFKVDPLKIAALLAGTFVLY
                     EPMKKLSRIHMLLQKALAASTHIFEILQTKSSIQDAPDAVAIRECLGRIEFDQVSFSY
                     GSNQNALSGISLAIEPGRQYALVGASGAGKSTILSLLLRFYDPQSGAIRLDGRDLRRL
                     TQKSLREHIGIVTQDTFLFHDSIYENIRYGRLDATREEIEAAAKLAFAHDFILAQPQG
                     YATVIGDKGCLLSGGQQQRLSIARALLKNAPVLLLDEATSSLDSESERMIQSALERLT
                     QGKTVIAIAHRLSTILKSDQIVVMNHGRIVEVGTHSHLLECSSVYRRLYELQFHHEPE
                     PAGA"
BASE COUNT         1244 a         2155 c         2092 g         1220 t
ORIGIN      
        1 ctggagcatt acctgcccag accgcagaaa accgaagccg aagtgctcgc ggcaatggcg
       61 caacgccacc gcgcaagaat ggccgcgtcg gcctccgcgc aaaaaagaaa ctcgacccag
      121 aaattaacaa agtagaatta aaaggcaggg acggctcgat gagccgtccg tcgcttcatt
      181 ccccccattc aggcaaagac gcacggattc ccgattccac ctcccacggc tccagcaaat
      241 gccggggcag ttcctccaaa aaacgcgacg gacgctggaa gacatcgccg taaccggcat
      301 tcaggcgcat ttccggatac gttaaataca gctcgtcctt gcagcgcgtg actccgacat
      361 aaaacaaccg ccgctcctcc tccagcgatt ccggattttc cacggatcgg gagctgggaa
      421 acatcccctc cgtcagccag atcaggaata cgacctgcca ttccaatccc ttcgcctgat
      481 gaatgctcga cagggtcacc ctctcctcgt tgcccgcgga ggcggtggcg tcggcggttt
      541 cgagcgcgcc cagcagggcg agctgggaca ggaattcctc ggcgctcgcg aaggatttgg
      601 aataactcgc cagcgtctgc aagtcctccc gccgcgcctc gtagttggcg aattgggttc
      661 gcatgtagtc gtcgtaaatc gccagcacga cggattggat catctccgcc ggaggcgcgg
      721 gctcgccgtt cgggaccagc tcctcgagcg tgtgcacgag ctgcttccag ccttccgccg
      781 ccttggccgg cggccggacg ccctcgagca gccgggcaaa atcccggccg ccttccagga
      841 tgcgctgcga cttttcccac agcgcttccg ccgatttccc gccgacgccg ggcagcaatt
      901 tgacgaagcg cttaaaggac atttcatcgc gcggattcac ggcgaatcgc agaaaggccg
      961 ccacgtcctt cacgtgcgcc tgctcgaaaa aacgcaggcc gctggtgatg gtgtagggaa
     1021 tgccgcggcg cgtgaattcg agctgcactt ccatcgaatg aaaatgcgag cgatacagca
     1081 cggccatttc cgaaaaggcg atcccctcct cgtggagttc cagcacgcgc tgggcggcga
     1141 acgcggcctg ctggttgttc gtgccgagct tgaccaggcc gggccgcacc cgcttctccg
     1201 cgcgcacggg gcggagcgcc ttttcgaatt gcgccaggtt gccggtaatg gacgcgttcg
     1261 ccagctccag cacctcgggc acgctgcggt agttggtctc gatcttgaaa accctcgcgc
     1321 cggggtaatg cttcggaaat tcgagaatgt tcagacagtc cgccccgcgc caggaataaa
     1381 tggactgcgc gtcgtcgccg acgaccatga ggttgccgtg cttgccggcc agccgcgcga
     1441 tcaattccgc ctgcagccgg ttggtgtcct ggtattcgtc cacgagaatc tgccggaatt
     1501 gattctggta gtgctcccgc acgtcgggat gggtttcaaa cagctccagc agcttgctca
     1561 gcagatcgtc gaaatccatg gaattcgcct cgcgcttgcg gccttcgtag gcggcctgca
     1621 cgcgctcgat cggctccagc agcggcgtaa aatagcggta gcgggtcgcg ataatgcgcg
     1681 cgagcggctg gcccgtgttg atcgcgaggc tgaaaagctc cgccagcacc gaacccttgg
     1741 gaaaacgttt gtcgttcgcg cgaatgcctt cgcgcaccac caccgcttcg aggagatcct
     1801 ccgagtcctc gcggtcgaga atggaaaatc ccggccggaa gccgatccgc tccgcgtggc
     1861 ggcgcaggat gcgattgccg atggaatgaa acgtgccgcc ccagatgccg gcggacatct
     1921 cgggcagcag cgcattcacg cggtccagca tttcgcgcgc ggccttgttc gtgaacgtca
     1981 gcagcaggat cgcgtagggc ggaaacccct gctccagcag ccaggcgacg cgatacgtca
     2041 gcgtccgcgt cttgccgctg cccgcgccgg cgatgaccag cgccgctccc ggcttcgacg
     2101 taacggcggc cagctgttgc tcattgagtt cgcggtcata ggcgatgcgc cggtgcgccg
     2161 gcggcgtttc gagggtgtaa cgcacgaatc aattggccga accgccgctt gaaacccgca
     2221 tggaagccac tccgtctttg gaaatcccca ggccgtaggc gagcagatcc accgattctt
     2281 tgcgcacccg cagcttcgtg tcgcccaaca ccacggcgat cacctgccgc ccgccccagc
     2341 tggcactggt gatcaggcaa tgcccggcgg cgatggtgta accggtcttc atgccgttgc
     2401 agaacgaata ctcctgcagc agccggttgg tgttccgcag ggtgacggtt ttgccgctgg
     2461 cgtaacgaaa ggtgatgaat ttctgcgaga tgatgtcgcg aagaaccgga ttcgcataca
     2521 cggcgcgcgc aacgatcgcc atatcccgcg cggtggaata ctggccctcg accggcagcc
     2581 cgttgggatt gatgaaatgg gaattggtgc cgcgcagttc cgcgattcgc gcgttcattt
     2641 tccgggcgaa agcctcgacg ctgccggcgt tgtcccgcgc gagcgcgcag gccacatcat
     2701 tggcgctctt caccagcaag gcggacaaca gctcctggcg cgtgtattgg tcgccggtct
     2761 tgagatacaa tttggtcgga tcggccaggg tgtcggattg ctcgaccgtc acgagctgat
     2821 ccaggccgcc cgcctcggcg atgatcagcg ccgtcagcag cttctgcgtg ctggccacgg
     2881 gccgctgggc attttcattt ttcttcagca gcacctcgcc ggaggtggcg tcgaaaagca
     2941 gcgcggcctt ggccgtgatc accggaggct ccgccgcttc gacgcggcag gccagcagcg
     3001 caagggcgag cgaaagggca acgattcgca tggggcggga gtttagcgca aaatggcgga
     3061 attcgacagc attttttcaa tttccgcatc tttttccgcg ccgagttcca atgcgcggag
     3121 gtaatggcgg cgggccagct cgaccgccgg ctttttgcgg tcgagataaa acagcgcgag
     3181 attgtagttc gcgtcggcgt atttctcgtc cagttcgatg gctttgcgca gctccgcttc
     3241 cgccgcgtcc agccagccga ggcggcccac caccacgccg aggtaattat gcgcgcgggc
     3301 gtttttcgga tcgtaaagcg tggcctggct caaggcggcc agcgcggcgt cgagtttgtt
     3361 ttgatccaga taaatcagcc cgaggatcag ccaggccggc gcggtttcga ttcgctgctg
     3421 cacggcctgc ttcaacagtt tttcggcggc gccgcgatct ccgagataaa actgggtcat
     3481 gcccaggttc acctgcgcga gcaggtaacg cggcgaaatc ttcagcaccg tttcgtaggc
     3541 ttcgcgggcc ttccggtaat cgccgtcggc aaaggcttcg ttgccctgcg agatcgcgag
     3601 cttcgcggaa tcgggcagat attcctcctg cgcaaacagc gttcccgcca ttcccgccag
     3661 cgcgatcaaa atcagcagtc ccgtgcgcaa tttatttttt ggcaacgctg cccagttcgg
     3721 ccgcccggcg cgcggaggct tccacggcgg caatgaacgc tccccgaacc ccgcggtctt
     3781 cgagcacggc cagcgccgcg gccgtcgttc cgcccgggct ggtgaccatc tcgcgaagca
     3841 gcgcgggatg ttgcttcgtt tccacggcca gttgcgccga tccgaggacg gtctggaccg
     3901 ccagctgttg cgccagatcc cgcggcagcc cggctttcac gccgccctcg gccaatgcct
     3961 ccatcaccag atacaaatac gccgggccgc tgccgctcag gccggtcacg gcgtccagga
     4021 gcgattcctt caccggatac accttgccca ccgacccgaa aagcagctcc gccgcgtgca
     4081 catccacctc ggtatgtttg gcgtcaaacg ccaccgccgt cgcgccggcc ccggccagcg
     4141 cggcgacatt cggcatcgca cgaaccaggc gcggcgaagc gccggtcatt tcgcgaagcg
     4201 tggaaatcgc aaggccgctc atcacggaaa cgacgagttt ggccttgagc gccgatttgc
     4261 aggactcgat ggccgccggc gcgtccgcgg gtttcactcc gaaaatgacc acatcccccg
     4321 cccccgccgc gctggcgttg tcgggcgcgg taacaatgcc aaactcctcc cgcagcgccg
     4381 cgagtttttc cggcgtccgg gtggaaacgt gaatgtcgcc cggcgcggcg actccgacgc
     4441 gcaacagccc ctgcaccagg gcgcgattca tccgcccggc gccgacaaag gaaaatttca
     4501 atccgctcgg aacactcatt ggatggaaaa attaacgcgc cggcccgaac agttcacgca
     4561 aaaaggcggc atcgtcgaaa tgccggctct ggcccaggcg caggacgacc agcttgcgcg
     4621 agggcaccac gtaaagccgc tggccgtagg aaccgatcat ggccaccatg tccctgggcg
     4681 ccgaccggct gaggcaggca tccctccaaa aggaaagctc cgtgcgttcg ccgatttccc
     4741 gttcgacatt gatttcgttt ccgcgggaaa gattccgcca gaaccccagc gcatagaccg
     4801 gcaggcccgc ccgcgggtcg tagatcgccc ccaggtccgc gcagaaccag cgcgtcaggc
     4861 cggggcggcc ggcattgagc acgatgtcgc caaagcgcag aaactgcggc accgaaagcc
     4921 gggcgccggt ggaaaaatac ggatttcccg acaagtccag ccgccagccc tcgatgctgg
     4981 cgccgatgta attaaacagc cgccgctgga aatacgacag cggggaaacg ccccgcgcct
     5041 tgagcttgcg agcgagcaat tcctccagaa cctcgaagtc ggcggggcca taggcgaatt
     5101 ggccgcccgg ctcgccggtc atgcccagcg cgacggcctc cttggccttg ttggcgatct
     5161 ttttgcggta gaggcaatga taacccggcg aaagccccgc ctgcaggttg agcaaatccc
     5221 ggatgcggat gtcgcgcttg gcgggctttt ttcgccactc cgtcagcgta tcgctcgcgc
     5281 gctcgtccag gtcgagcagc ccatcattcc gcgccgcgag aaacagcgtg ccgaccaggc
     5341 tcttggtgat gctcaggcag gcgtaacgct cgcccctccg cgccgccatg ctttgttcat
     5401 cgtgaaccgt gcggccggcc tcgcggatga ccagcacctc cccgccgtgc ctccgggaat
     5461 aatccgcggc ggagtcgatc cgcaggggag acagcgcgag caggagcagg ccgaaggcga
     5521 tcatgcgccg gccggttcgg gttcgtgatg gaactgcagc tcgtaaagcc ggcgatacac
     5581 gctggaacac tccagcagat gcgaatgcgt gccgacctcg acaatgcggc catggttcat
     5641 gaccacgatt tggtccgatt tcagaatggt ggacaggcgg tgcgcgatgg cgatgaccgt
     5701 cttgccctgg gtgaggcgtt cgagggcgga ctggatcatg cgctccgatt ccgaatccag
     5761 cgacgaggtg gcttcgtcga gcaggagcac gggggcgttt ttcagcagcg cgcgggcgat
     5821 ggaaagccgt tgctgctggc cgccggagag caggcagccc ttgtcgccga tgaccgtggc
     5881 gtaaccctgc ggctgggcga ggatgaagtc atgcgcgaag gcgagcttcg ccgcggcctc
     5941 gatttcctcg cgcgtggcgt cgagccgtcc gtagcggatg ttttcgtaaa tgctgtcatg
     6001 gaacaggaac gtgtcctgcg tgacgatgcc gatgtgctcg cgcagggatt tctgcgtgag
     6061 gcggcggagg tcgcggccat ccagccggat cgcgcccgat tgcggatcgt agaagcgcag
     6121 cagcagcgaa agaatggtgc tcttgcccgc gccgctggcg ccgacgagcg cgtattgcct
     6181 gcccggttcg atggccagcg aaatcccgct gagcgcgttt tgattcgatc cgtaggaaaa
     6241 gctcacctga tcgaactcga tgcggcccag gcattcgcgg atggcgaccg cgtccggcgc
     6301 atcctgaatg gaagatttcg tctgcaggat ttcgaagatg tgcgtggagg cggccagcgc
     6361 cttttgcagc agcatgtgaa tgcggctgag ctttttcatc ggctcgtaga gcacgaaggt
     6421 tcccgccagc agcgcggcga tcttgagcgg atccaccttg aagaaaaaga cgtagagcag
     6481 cgcgagcgcg atgccgagcg cggaaaccgt ctcgatcagc ggctgcacga tgtccgagct
     6541 cttgcggacc ttcaggctga tgcggaattg ctcctggctg gatttggcga attggtcggc
     6601 ctgatagttc tcccgggcga aggatttgat cacgcggatg cccgccagcg tttcgtgcag
     6661 gatgaccgac atggcgccgg cttcattttc ctccgccttg cccgcctggc g
//
LOCUS       DATDAY010000326         4026 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_1527596, whole genome shotgun sequence.
ACCESSION   DATDAY010000326 DATDAY010000000
VERSION     DATDAY010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 4026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4026
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1527596"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            339..2144
                     /locus_tag="VIS74_06480"
     CDS             339..2144
                     /locus_tag="VIS74_06480"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEY5792927.1"
                     /translation="MATLEGTNGNDNLEVGWEENVFGYDGDDTISLNPFTDRIYHADG
                     GKGTDILNVDFSFSTHAPGSGGFAYSWMGFDANAGDASAYHSEDIYEWVYDPDTGEVS
                     YEYIYHLEEYAGVSFEGFELFNVTGTFASDSIVTGAGNDTLSALSGDDHLDGGAGNDS
                     LNGGAGADTMIGGTGNDTYVVDTVFDVITETSAVAAEIDLVQSSISCTLGANLENLTL
                     LGTAAVNGTGNALANFLTGNGARNILHGGDGNDSIDGGSGNDALIGGTGNDKFFGADG
                     NDYLDGGEGGDYVHGGTGADTLIGGTGNDTYVVDNSGDIVSEMSTAAVEIDLVLSRRS
                     YTLGARLENLTLFGTATIDGTGNALANKITGNSGNNKLDGGAGNDSLSGGVGHDGLNG
                     GAGKDTLAGGAGNDTYVVDTVFDVIIETSTLATEIDLVQSSITHALGVNLENLTLTGS
                     ANSAGTGNALANKITGNRGNNRLSGGDGNDILTGASAATFGVGELDTLTGGVGDDRFV
                     LGNSAGRFYDDGSTATAGTVDYAFITGFTARSDRLQLDGTAANYYLGASPVAGVSGSA
                     LFYENGAADELVAIIKSGNSTTLTAANTINTAAFV"
     gene            2208..2363
                     /locus_tag="VIS74_06485"
                     /pseudo
     CDS             2208..2363
                     /locus_tag="VIS74_06485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016721870.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            2519..>4026
                     /locus_tag="VIS74_06490"
     CDS             2519..>4026
                     /locus_tag="VIS74_06490"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEY5792928.1"
                     /translation="MIQFRTLVFLVVGGLAGLGRAADLTFLNGAEPETLDPAIITGQP
                     EGRVVNALFEGLLRYDAKGRGIPGTAESWEISPDGKTYTFHLRDNARWSDGRPVTAED
                     FVRSWRRTLSPATASPYNYMLFPIRHAEAFAEGKLADFHEVGVKALDARTLRVELAHA
                     TSYFLDLCAVPALHPVPVDLIDHVGDAWVKPEHIVTNGPFRLEKWRLNDSITLRKNPD
                     YWDAAAVSLDEIRVLPISKAEVALNFYASGGADLMMDKGLAPPALLDELVKRPDFHAA
                     PFLGIYFIRFNCEKPPFNDARVRQAFSLVVDQNRIVKGITRAGELPAASFVPPGIEGY
                     PAAEGLRRDPERARKLLAEAGFPGGRGFPLVSYLYSEGALNEAISVELQSMWRRELGV
                     NVNLDRQEWKVYLNSLNSLDYDIGRSSWVGDYPDPTTFLDMLVTNNGNNRTGWSSEKY
                     DSLIRESGRELVPARRFERLRAAESFLLREGTPIFPIYYYVGIQIYDGAKFGG"
BASE COUNT          848 a         1150 c         1255 g          773 t
ORIGIN      
        1 atggtgcaag tctccgctcc acgccccccc ctccaagcct gctggagctc catcttcccc
       61 tttggacaaa gcgccgggtc aacaatgatc accccatcga cctcgagggg aaaaattacc
      121 agattgccgc taccgctctc cagtccgtta ccatcattcc aaccgtaaat tttgggttgc
      181 cgatcacacc ccaaacgctg cctggccatc tatttaggag ctttcacgct ctaaaaaact
      241 gtccagtttt gaaccgtcgc cgacaggccg tcttcattct cttgcaatcc tgaaaaatat
      301 acatcatgtt agccggggaa tcaaaaactc taaacgctat ggcaaccctc gaaggcacca
      361 acggcaacga caatcttgaa gtaggatggg aagagaatgt cttcggatac gatggtgatg
      421 ataccattag tctgaatcca ttcacggacc ggatctatca tgcggacggc ggcaagggaa
      481 cggatatcct taatgtcgat ttttcctttt ccacacatgc tccgggttct ggtggctttg
      541 cttactcatg gatgggtttt gatgcaaacg caggggatgc cagtgcctac cacagtgaag
      601 atatctacga atgggtttac gatccagaca ctggcgaagt gtcctacgag tacatttacc
      661 atttggagga atatgcaggt gtttcttttg agggttttga actattcaac gtcactggca
      721 cctttgcctc tgattcaatc gtcactgggg cgggaaacga taccctgtct gccctgtctg
      781 gtgacgacca cctcgacggt ggagcgggca acgacagttt gaacggcggc gcgggagccg
      841 atacgatgat cggcggaacc ggcaacgaca cctacgtggt ggacacggtc ttcgacgtca
      901 tcaccgagac aagcgccgtg gccgccgaaa tcgacctggt gcaaagcagc atatcctgca
      961 ccttgggagc gaacttggaa aatctcacgc tgctcggcac ggccgccgtc aacggtacgg
     1021 gcaatgcgct ggccaacttc ctcaccggca acggagctcg caacatcctg catggcggag
     1081 acgggaacga tagcattgat ggcggcagcg gtaacgacgc cctcatcggt ggaaccggta
     1141 acgacaagtt ttttggcgcg gacggcaacg actatctcga cggcggagag gggggcgact
     1201 acgtccacgg aggcaccgga gccgatactc ttatcggggg aacgggcaac gatacttacg
     1261 tggtggacaa ctccggcgat atcgtctccg agatgagcac ggcggctgtc gaaatcgact
     1321 tggtgctaag ccgcagaagc tacaccttag gcgccagact tgaaaatctc accctgtttg
     1381 gaacggctac catcgatggt acaggcaacg ctcttgccaa caagatcacc ggcaacagcg
     1441 ggaataacaa acttgatggt ggcgcgggca acgacagcct ctctggaggt gtgggccacg
     1501 acggtctcaa cggtggcgcg ggaaaagaca cccttgccgg cggcgccggc aacgacacct
     1561 acgtggtgga taccgttttc gacgtgatca tcgagacgag cacgctggcc acggagatcg
     1621 acttggtgca aagcagcatc acccacgctc tgggagtaaa cctcgaaaac ctcacgctca
     1681 cgggctcggc caatagcgcg ggcacgggca acgccctggc caacaagatc accggcaacc
     1741 gcgggaataa ccggctcagc ggaggcgacg gcaacgatat attgacagga gccagcgccg
     1801 ccaccttcgg cgtgggcgaa ctcgatacgc tcacgggagg tgtgggcgac gaccgtttcg
     1861 ttctcggcaa tagtgcaggc cgtttttacg acgacggctc caccgcgacg gcaggcacgg
     1921 tggactacgc gttcatcacc ggcttcacgg cccggagtga ccgcctgcag ctcgatggaa
     1981 ccgcggccaa ttattacctg ggggcgtcac cggtggccgg cgtgagtggc agcgcgttgt
     2041 tctacgaaaa cggggccgcg gacgaacttg tggcgatcat caagagtgga aactccacca
     2101 cgctgacagc cgcgaacacc atcaacacgg ctgcgtttgt ttagggaaac gcggattaaa
     2161 tcggcgagca gagttgtgga gaggggagcc gggaggtaaa agagggggtg gagagccagc
     2221 taagttttgc ggcgagcgaa tggagacgga agaagcagac gaagagagag aagtttttga
     2281 agccgatgga ggagttggtt ccctgggaga ggctggcggc attgatcgag ccgcattatc
     2341 cgaaagggga gcggggacgg ccgcccaaag ggattgagcg ggtgctatat ccgatgagca
     2401 gccaggccgg catctcattc atcaaaactc ggtgagttct ttggattcct ttgcggcggc
     2461 ggaatgactt ttgcggaaaa aactgtccat ttcggtccag tgtggtttga gtttttccgt
     2521 gatccaattt cggaccctcg tttttctggt ggtgggcggg ctggcggggc tcggccgcgc
     2581 ggccgatctg accttcctca atggcgcgga gcccgaaacg ctcgatccag ccatcatcac
     2641 cggccagccc gaggggcgcg tggtgaatgc gctctttgaa ggattgctgc gttacgacgc
     2701 gaagggccgc ggcattccgg ggacggcgga gagctgggaa atctcgccgg atggcaagac
     2761 ctacacgttc cacctgcggg acaatgcgcg ctggagcgat ggccgtccgg tcaccgcgga
     2821 ggatttcgtc cgatcctggc ggcgcacgct gagcccggcc accgcctcgc cttacaatta
     2881 catgctcttc ccgattcgcc acgcggaggc ttttgccgag ggaaaactcg ccgattttca
     2941 cgaggtgggc gtgaaggcgc tggatgcccg gacgctgcgg gtggagctgg cccacgcgac
     3001 ctcctatttt ttggatctct gcgccgttcc cgcgctgcac cccgtcccgg tggacttgat
     3061 cgaccacgtg ggggatgcct gggtgaagcc ggagcacatc gtgaccaatg gccccttccg
     3121 cctcgaaaaa tggcggctga atgactcgat cacgctccgg aagaatccgg actattggga
     3181 cgccgccgcc gtgtcgctgg acgaaatccg ggtgctgccg atcagcaagg ccgaggtggc
     3241 cctgaatttc tacgccagcg gcggggcaga tttgatgatg gacaagggcc tggcgccgcc
     3301 ggccctgctg gatgagctgg tgaagcgccc ggacttccac gccgcgccgt ttctcggcat
     3361 ctattttatc cggttcaatt gcgagaagcc gccgttcaac gacgcgcggg tgcggcaggc
     3421 gttttccctg gtcgtggacc agaatcgcat cgtgaaaggc atcacgcgcg ccggcgagct
     3481 cccggcggcc tccttcgtgc cgccgggaat cgagggatac cccgccgcgg aaggcctgcg
     3541 gcgcgacccc gagcgggcgc ggaaattgct cgcggaggcg ggcttcccgg gcggcagggg
     3601 cttccctctg gtgtcctatc tctacagcga gggggcgctg aatgaagcga tctccgtcga
     3661 gctccagagc atgtggcggc gggagctcgg cgtgaacgtc aacctcgacc gccaggagtg
     3721 gaaggtgtat ctgaattcgc tgaactcgct ggattacgac atcggccggt cgagctgggt
     3781 gggggactat ccggacccga ccaccttcct cgacatgctg gtgaccaaca acggcaacaa
     3841 ccgcaccggc tggagcagcg aaaaatatga ttcgctcatc cgggaatccg gccgcgagct
     3901 ggttcccgcg cgccgtttcg agcggctgcg cgcggcggaa tcctttttgc tgagggaagg
     3961 cacgccgatc tttccgatct attattacgt gggaatccag atttacgatg gcgcgaagtt
     4021 cggcgg
//
LOCUS       DATDAY010000327         2923 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_1352076, whole genome shotgun sequence.
ACCESSION   DATDAY010000327 DATDAY010000000
VERSION     DATDAY010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2923)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2923)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2923
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1352076"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(105..1184)
                     /gene="prfA"
                     /locus_tag="VIS74_06495"
     CDS             complement(105..1184)
                     /gene="prfA"
                     /locus_tag="VIS74_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEY5792929.1"
                     /translation="MDFNLLIERKRERFEQLEREIADPALFENRKRASEIMREHSSVK
                     QLLARWTELETARQQLDDNRELAESSDIEIAAMADDEIPELQKRVTELERDFQIALLP
                     PDENENRDAIIEIRAGTGGSEAAIFAADLYRMYNRYAEAAGLKIEDLESSPSELGGLK
                     ESIFKVSGENVFRQLRFESGVHRVQRVPATEAQGRIHTSTATVAVLPEAEEVDLELKP
                     EDLRIEVCRSGGPGGQGVNTTDSAVQILHIPTGRIVRCQDGRSQQKNKEKALKIMRAR
                     LLEEKQREEEAKYSAHRKNQIGSGGREEKIRTYNYAQNRVTDHRIGLTLYNLDRFMEG
                     DVEEMIKALQAADLQERLQESSITA"
     gene            complement(1253..1486)
                     /gene="rpmE"
                     /locus_tag="VIS74_06500"
     CDS             complement(1253..1486)
                     /gene="rpmE"
                     /locus_tag="VIS74_06500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010242421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HEY5792930.1"
                     /translation="MKADIHPEYYETDIACACGAVYHTRSTKRDIKIGICAACHPFFT
                     GEQKFVDTAGRVEKFARRYGSTKATRAGAAPKA"
     gene            1537..2871
                     /locus_tag="VIS74_06505"
     CDS             1537..2871
                     /locus_tag="VIS74_06505"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HEY5792931.1"
                     /translation="MKLDDALLAEWEKTVARKGEAAAILDTQGEVLRTFNDIERQARS
                     LEAQIDGCSPGQVHAIDIGNHPDWPSLLLACLRRQVVVLPLDESLTADQREAARQICA
                     GKASANWGEHPPVLLKLTSGTTATPRAIRFRSEQLLADCRQICETMGITDRDLNFAVI
                     PLSHSYGLSNLLTPLLVFGVPMVLSRDRMPRAVLDDLARTGATVFPGMPVFYQAFSEM
                     ADVPALPQLRLCISAGAPLPLEVARKFRQKFNQPIHSFYGSSECGGICYDRDALLLEP
                     GFVGQPMCGVDLDLLEPDASESRLRVRSAAVGDGYFPEPDEEKLGHGRFAPDDLLSAS
                     GDGFRIVGRISDVINVAGKKVNPAEVEAELLRFDGVRAAVVFGRQSVLRNEEVAACVV
                     AEERVRESDLLEHCRERLSGWQVPKRIFFVTEIPANERGKVNRRELATRFAS"
BASE COUNT          522 a          892 c          886 g          623 t
ORIGIN      
        1 ttgaagcacc tcgagcacag tcattgagaa attgggaatg tagaggcgct ccgcggcgag
       61 cgcctgttgt tctggtcagg cgcttggcac aaccgcctcc aaatttaagc ggtgatcgac
      121 gattcttgca gccgctcctg caaatccgcc gcctggagcg ccttgatcat ctcctccacg
      181 tcgccttcca tgaaacgatc cagattgtag agggtcaatc cgatgcggtg atcggtcacc
      241 cggttttggg cgtaattgta ggtgcggatt ttttcttcgc gcccgccgga accgatctgg
      301 ttcttgcgat gcgcggaata cttcgcctct tcttcgcgct gcttctcttc cagcagccgc
      361 gcccgcatga tcttgagcgc tttctccttg ttcttctgct ggctgcgccc atcctggcaa
      421 cgcacgatcc gtccggtcgg aatatgaaga atctgcacgg cggaatcggt cgtgttcacg
      481 ccctggcccc cgggaccgcc cgagcggcag acttcaatcc gcaaatcttc cggtttcagt
      541 tcgagatcga cttcctccgc ttccggcagg acggccacgg tcgccgtgga ggtatgaatg
      601 cgcccctgcg cttcggtggc cgggacgcgt tgcacccgat ggacgccgct ttcaaaacga
      661 agctgccgga aaacgttttc gcctgaaacc ttgaaaatgg attccttcaa cccacccagc
      721 tcggatggac tcgactcgag atcttcgatc ttcaatcccg ccgcttccgc gtagcggttg
      781 tacatccggt aaagatcggc ggcgaagatc gcggcttcgc ttccacctgt ccccgcgcgg
      841 atttcaataa tcgcgtcacg attttcgttc tcgtcgggag gcagcagcgc aatttggaaa
      901 tcgcgctcga gctccgtcac gcgtttctgc agttcgggaa tttcgtcgtc ggccatggcg
      961 gcgatctcga tgtcgcttga ttccgccagc tcccggttgt cgtccagctg ctggcgcgcc
     1021 gtctccagct cggtccagcg cgcgagcaac tgcttcacgc tggaatgttc ccgcatgatc
     1081 tcgctggcgc gtttccggtt ctcgaaaagg gccgggtcgg cgatctcgcg ttcgagttgc
     1141 tcgaaacgct cgcgcttgcg ctcgattaac aaattgaagt ccataagacg ctgtagccgc
     1201 gatcgctgac cgcggcaggc cggggtcggc gaccccagct acagaaattc agctaggctt
     1261 tgggagcagc gccggcccga gtggccttgg tgctgccgta acggcgggcg aatttctcga
     1321 cgcgacccgc ggtatccacg aacttctgtt cgccagtgaa aaacggatgg cacgccgcac
     1381 aaatgccgat tttaatgtcc cgcttggtcg agcgtgtgtg gtagacagca ccgcacgcgc
     1441 aggcgatgtc ggtctcgtaa tactctggat gaatgtccgc tttcatgttt ggaaacgagt
     1501 ggcgaactta ggcgcgctcc gggacgcgcg caagggatga aattggacga cgccttgctg
     1561 gccgaatggg agaaaacagt cgcccgcaag ggagaggcgg cggccatttt ggacacccag
     1621 ggagaggtgc tgcgaacctt caacgatatc gaaaggcagg ctcgtagcct ggaagcccag
     1681 atcgacgggt gcagcccggg ccaggttcac gccatcgaca ttggcaacca tcccgactgg
     1741 ccttccctcc tgctcgcctg cctccgccgg caagtggttg tcctgcccct cgacgagtca
     1801 cttaccgcag atcagcgcga ggcggcgcgg caaatttgtg ctggcaaggc ttcagccaat
     1861 tggggcgagc atccgccagt tctattgaaa ctgacctccg gcaccacggc cacgccgagg
     1921 gccatccgtt ttcggagcga gcaattgttg gctgattgcc ggcagatttg cgaaacgatg
     1981 ggcatcactg accgagacct caatttcgcc gttattcccc tctcgcactc ctatggcctg
     2041 agcaatctgc tcacgccgct gctggttttc ggcgtgccaa tggttctgag ccgggaccgc
     2101 atgccgcggg cggtcctgga cgatctcgcc cgcacaggcg cgacggtttt ccccggaatg
     2161 ccggtttttt atcaggcgtt ttccgaaatg gcggacgtgc cggcgcttcc gcagttgcgg
     2221 ctttgtattt cggcgggcgc gccgctgcca ctggaggtgg cgagaaaatt ccgccagaaa
     2281 ttcaaccagc ctatccattc cttttacggg tcctcagaat gcggcggcat ctgttacgat
     2341 cgtgacgcgc tcttgctcga gcccggtttc gtcggccagc cgatgtgcgg agtggacctc
     2401 gacctgctcg agccggacgc ttccgaaagc cgcttgcgcg tccgaagcgc cgccgtcggg
     2461 gacgggtatt ttcccgagcc cgatgaagaa aaactggggc acggccgctt cgctccggac
     2521 gacttgctga gtgcgagcgg ggacgggttc cgaatcgtcg gacgaatttc cgatgtcatc
     2581 aatgtcgcgg ggaaaaaggt gaacccggcg gaagtggaag cggaactcct gcgctttgac
     2641 ggcgtccgcg cggcggtcgt cttcggtcgc caatcagttc tgcgcaacga agaagttgcc
     2701 gcctgcgttg tggcggaaga gcgggtacgg gaatccgatc tgctcgagca ctgccgagag
     2761 cgcctcagcg gctggcaggt ccccaagcgg atttttttcg tcacagaaat cccggcgaac
     2821 gagcgcggca aagtgaatcg ccgcgagctg gcgacacgat ttgcgtcctg aagtcacctc
     2881 cggcaggggg ttgtagccgc ggtcgttgac cgcggacctc tcg
//
LOCUS       DATDAY010000328         4969 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_2410702, whole genome shotgun sequence.
ACCESSION   DATDAY010000328 DATDAY010000000
VERSION     DATDAY010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 4969)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4969)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4969
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2410702"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..399
                     /locus_tag="VIS74_06510"
     CDS             <1..399
                     /locus_tag="VIS74_06510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHHA2 domain-containing protein"
                     /protein_id="HEY5792932.1"
                     /translation="KLAEEIFSVGSPLLTMKPAQAVSADCKIYEERGLSFSVSQIEEL
                     SFSHLPERQAALLDALEEYRQKNGLIFSTLLVTDVNTQNSILLLRGSARFEQLVDYPE
                     MDAHTWQLDGIVSRKKQLLPYLTTLLGRLA"
     gene            396..1169
                     /locus_tag="VIS74_06515"
     CDS             396..1169
                     /locus_tag="VIS74_06515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEY5792933.1"
                     /translation="MKKFALLAATLLLASCGLKPSNDLRIGMDLSYPPFEMRDAEGRP
                     AGISVEMAKALGEYLGRPVRIENIPFTGLIAALKGYRIDLILSSLTATEERRQSIDFS
                     DPYLRVGLALLVNRHSGIRSLADADQPDKTVAVRQGTTAQLWAAQHLKRAKILTLEKE
                     NAAVLEVIQGKADAFIYDQISVWNYWRQNPETTEALLEPLQSESWAIGLRQKDEALKV
                     QVNQFLKAFREQKGFDRLGDQFLGDQKKEFQARGIPFYF"
     gene            1177..1947
                     /locus_tag="VIS74_06520"
     CDS             1177..1947
                     /locus_tag="VIS74_06520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEY5792934.1"
                     /translation="MKRIFLDQRENPAPPAWQALHLAALLLLFSLISWALLARTDLKW
                     EAVWKYRGVFFSGWLLTIGLSFAALLLSLLIGAASALASRSSFLPLRSLAVLYVELVR
                     GTPLLAQIFWFTYVVFGTLPVDRLFVGVLSLACFTGAYISEILRAGIQSIGESQLESA
                     RAIGFTPLQTYRFVIFPQALRQALPPLAGQFASLIKDSSLLSVIGISEFTLAAQQSAS
                     ATYTTLESYLPLALGYLALTVPIFLFTRWLERRSHFLT"
     gene            1947..2681
                     /locus_tag="VIS74_06525"
     CDS             1947..2681
                     /locus_tag="VIS74_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEY5792935.1"
                     /translation="MRLEITNLTKHFGAHRVIDVPAFTAEFARCLALVGPSGGGKSTL
                     LRLIGGLETPDTGTIRVENEPLPAEETRRREYRRRIGFVFQGLNLFPHLTALENVTLP
                     LTQIHRIPPGEADSRARGVLRRFGLEAHVHKKPGQLSGGQRQRVAIARAIAIQPRFLL
                     LDEPTSALDPEMTVEVLETLEELREQGVAFILVTHEMGFARRAADRVAFVENGRIACC
                     EPAAAFFERSESEKVRRFLAKTLKFA"
     gene            2691..4877
                     /locus_tag="VIS74_06530"
     CDS             2691..4877
                     /locus_tag="VIS74_06530"
                     /inference="COORDINATES: protein motif:HMM:NF044972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HEY5792936.1"
                     /translation="MRFAILFFSGLLALASPSPAQTKSFQSLIQQFTGAEAGANAGPP
                     PAEQLDWVKEQIKASRDQLQQLQNEESPAELIALAEQIVRNQTFAQTTLEGVIRSESE
                     LSKLRDQPARPPLAAPAELAAAKQSAADLKSQLAAASSEQPLLQQALSEAAAAVQTNQ
                     AALLELKDAPPAQTRLAQLRAAASETALYNLKWRLYLEALHSQANEIQLQQLERGIDL
                     SRLDVQINAGRAKEALAQLEKDRAQITEKLAAAQKTSEALSQQLKSLEEQTRAGNSSK
                     AVQSQIQILKASQNHANQLAIGLDHMLQLMDLEVKHWNSVLELSATKDPSLFQQTREA
                     IQGDLNRISQIKAVLLQRFQDTTRALEQLANRADANWQEKSLRPLLEKQQEIVGSRVE
                     MLRAQLQRLEQIRSHAETLAAEVGARLLEQSFREKLAVSLGNAGSTLAGAWNFRLFTS
                     QGTNFTVGKLAVGITGLIAAFMLAAFISRRVAGTFSRRFHADATKAVLIEKWVYYLAL
                     VLLVLTVLNWLSIPLTVFAFLGGALAIGIGFGGQNLMNNFISGLILLFERRVNVGDIV
                     EVDGHTGKVMHLGSRSSRIRKWDGVEVLVPNSYFLEKNVINWTLSDPNHRYDFVVGVA
                     YGSPLKHCLEIFQKVLARHPHILPSPAPFVLFESFGDNALNFHFYYWLPVNVPHIDTG
                     AVGSELRLEIDAVCREANIEIAYPQRDVHLFTAKPIEVKVQSEKSA"
BASE COUNT          919 a         1649 c         1484 g          917 t
ORIGIN      
        1 aaactggccg aggaaatttt ttcggtcggc tcgccgctgc tcaccatgaa gccggcgcag
       61 gcggtttcgg ccgactgcaa gatttacgag gaacgcgggc tgagcttcag cgtgtcgcag
      121 atcgaagagc tcagcttctc gcacctgccc gagcgccagg ccgcgctgct ggacgccctg
      181 gaggaatacc gccagaagaa cggcctgatt ttctccaccc tgctggtcac ggatgtgaac
      241 acgcagaact cgatcctgct gctgcgcggc agcgcgcgct ttgaacagct cgtggattat
      301 cccgagatgg atgcccacac gtggcagctc gacggcatcg tttcgcgcaa aaaacaattg
      361 ctgccgtatc tgaccaccct cctgggccgc ctcgcgtgaa aaaattcgcg ctgctcgccg
      421 cgacgctcct gctggcttcg tgcgggttga agccttcgaa cgatctgcgg atcggcatgg
      481 atttgtccta tcctcccttc gaaatgcggg acgcggaggg gcggcccgcc ggcatcagcg
      541 tggaaatggc caaggcgctg ggggaatacc tcgggcggcc cgtgcggatc gaaaacatcc
      601 cgttcaccgg attgatcgcc gcgctcaagg gttaccggat cgatctcatc ctctcctcgc
      661 tgaccgcgac ggaggagcgg cggcagtcca ttgatttttc cgacccctac ctgcgcgtcg
      721 gcctcgcgct gctggtgaac cggcattccg gcatccgttc cctcgcggac gccgaccagc
      781 cggataaaac cgtcgccgtc cggcagggga cgaccgcgca gctctgggcg gcccagcact
      841 tgaaacgcgc gaaaattctc acgctcgaaa aggagaacgc cgccgtgctg gaggtgatcc
      901 agggcaaggc ggatgcgttc atctacgatc agatttccgt gtggaattat tggcggcaga
      961 atcccgaaac aaccgaagcg ctgctcgagc cgctgcagag cgaaagctgg gcgatcggcc
     1021 tgcgccagaa ggacgaggcg ctgaaggttc aggtgaatca attcctgaaa gccttccggg
     1081 agcaaaaggg cttcgaccgg ctgggcgatc aattccttgg cgatcaaaag aaggaattcc
     1141 aggcgcgcgg cattcccttt tatttctgag cggaaaatga agcggatttt tttggatcaa
     1201 agggaaaatc cggcgccccc cgcctggcag gcgctgcacc tggcggcgct gctgctgttg
     1261 ttctcgctga tttcctgggc gctgctggcc cggacggatc tgaaatggga agccgtctgg
     1321 aaataccgcg gcgtcttttt ctccggctgg ctgctgacga tcgggctgtc tttcgccgcg
     1381 ttgctgctca gcctgcttat tggcgcggcc agcgccctgg cgagccggtc ctcctttctg
     1441 ccgctgcgct cgctggccgt gctctacgtc gaactcgtgc ggggaacgcc gctgctggcg
     1501 cagatttttt ggttcaccta cgtggtgttc gggacccttc ccgtggaccg cctgttcgtc
     1561 ggggtgctca gcctcgcgtg cttcaccggc gcttacattt cggaaattct gcgcgcgggc
     1621 atccaaagca tcggcgaatc gcagctggaa agcgcgcggg ccatcggctt cacgccgctg
     1681 cagacctacc gcttcgtcat ttttccccag gcgctgcgcc aggcgctgcc gccattggcg
     1741 ggacagttcg cctccctgat caaggactcc tcgctgcttt ccgtcatcgg catttccgaa
     1801 ttcacgctcg ccgcccagca atccgcctcg gcgacttaca ccacgctgga gagctacctg
     1861 ccgctggcgc tggggtatct cgcgctgacg gttcccattt ttctgttcac ccgctggctg
     1921 gaacgccggt cgcatttcct cacctgatgc ggctcgaaat caccaatctc acgaaacatt
     1981 tcggcgccca ccgggtcatc gacgtgccgg cgttcaccgc tgagttcgcc cgctgcctgg
     2041 ccctggtcgg gccctcgggc ggcggcaagt ccacgctgct gcggctgatc ggcggactgg
     2101 agacgcccga cacgggaacg atccgcgtcg aaaatgaacc ccttcccgcc gaggaaaccc
     2161 gccgccgcga ataccgccgg cgcatcggtt ttgtgtttca gggactcaac ctgtttccgc
     2221 atctcaccgc gcttgaaaac gtcacgctgc cgctcacgca gatccaccgg attccccccg
     2281 gcgaggcgga ctcccgggcg cgcggcgtgc tgcggcgttt cggcctggaa gcgcacgttc
     2341 acaaaaagcc cggccagctc tccggcgggc agcggcagcg cgtggcgatc gcccgcgcga
     2401 tcgcgatcca gccgcgattt ctgctgctgg acgaacccac ttccgcgctc gatcccgaga
     2461 tgaccgtgga agtgctcgaa acgctggaag agctccgcga acaaggcgtc gcgttcattc
     2521 tggtcacgca tgaaatgggg ttcgcccggc gcgcggccga ccgcgtggcc ttcgtggaga
     2581 atggccgcat cgcctgctgc gagccggccg ccgccttttt cgagcgttcg gaaagcgaaa
     2641 aggtgcgccg ctttctcgca aaaaccctca aattcgccta ggttccccga atgcgtttcg
     2701 ccattttatt cttcagcggg ttgctcgcgc tcgccagccc ctcgcccgcg cagacgaaaa
     2761 gttttcagtc gctcatccag caattcacgg gcgcggaggc cggcgcgaat gcgggtccgc
     2821 ccccggccga gcagctggat tgggtcaagg agcagatcaa ggcttcccgg gaccagcttc
     2881 agcaactgca aaatgaggaa agcccggcgg agctcatcgc gctggcggaa caaatcgtcc
     2941 gcaatcaaac gttcgcccaa accacgctgg agggagtcat tcgatcggag agcgaattaa
     3001 gcaaacttcg ggaccagccc gcgcgcccgc cgctggccgc tccggcggag ctggccgccg
     3061 ccaaacagtc cgccgccgat ctcaaatccc agctcgccgc cgccagctcc gagcagccct
     3121 tgctccagca ggcgttgagc gaagcggccg ccgccgtcca aacgaaccaa gccgcgctcc
     3181 tggagctgaa ggacgcccct cccgcgcaaa cccggctcgc ccaattgcgc gcggccgcca
     3241 gcgaaaccgc gctctacaac ctcaaatggc gcttgtatct ggaggcgctc cattcccagg
     3301 ccaacgagat ccagcttcag caattggagc gcggcattga tctcagccgc ttggatgttc
     3361 agatcaacgc cggccgcgcc aaggaggcgc tcgcgcagtt ggaaaaggac cgcgcccaga
     3421 tcaccgagaa actcgccgcg gcgcagaaga cctccgaagc cttgagccag cagctcaaat
     3481 ccctcgagga gcaaacccgg gcgggcaatt cctcgaaggc ggttcaatcc caaatccaga
     3541 tcctgaaggc ctcccagaac catgccaatc agctcgccat cggcctggac cacatgctgc
     3601 agttgatgga cctcgaggtg aagcactgga attccgtgct ggagctaagc gccacgaagg
     3661 atccctcctt gttccagcaa acgcgcgaag cgatccaggg cgacttgaat cggatttccc
     3721 agatcaaggc cgtcctgctg caacggttcc aggacaccac gcgcgcgctg gagcaactgg
     3781 ccaaccgcgc cgatgcaaac tggcaggaaa aatccctgcg tccgttgctg gaaaagcagc
     3841 aggaaatcgt cggcagccgc gtcgagatgc tgcgcgcgca gttgcagcgc ctcgagcaaa
     3901 tccgctccca tgcggaaacc ctggccgccg aggtgggcgc ccggctgctc gagcagagct
     3961 ttcgggaaaa gctggcggtg tcgctgggca atgccgggtc cacgctggcc ggcgcctgga
     4021 acttccggct tttcaccagc cagggcacca acttcacggt cggcaagctg gccgtcggga
     4081 tcacggggct gatcgccgcc ttcatgctgg cggccttcat ctcccggcgg gttgccggca
     4141 ccttcagccg ccgctttcac gcggatgcca cgaaggcggt tctcatcgaa aaatgggtct
     4201 attatctcgc gctggttctg ctcgtgctga cggtgctgaa ctggctgagc attccgctca
     4261 ccgtcttcgc cttcctcggc ggcgcgctgg ccatcggcat cggcttcggc gggcagaatt
     4321 tgatgaacaa tttcatcagc gggctcatcc tgctgtttga acgccgggtc aatgtcggcg
     4381 acatcgtcga ggtggacggc cacaccggca aagtgatgca tctcggcagc cgcagctcgc
     4441 gcatccgcaa atgggacggc gtggaggtgc tcgtgcccaa cagctatttc ctcgaaaaga
     4501 acgtcatcaa ctggacgctc tccgacccga atcaccgcta cgacttcgtc gtgggcgtgg
     4561 cctacggctc accgctgaag cactgcctgg aaatcttcca aaaggtgctc gcccgccatc
     4621 cgcacatact tccgtcgccc gcccccttcg tgctctttga aagtttcggc gacaacgcgc
     4681 tgaactttca tttttactac tggctgccag tgaacgtgcc gcacatcgac acgggggccg
     4741 tcggcagcga gctgcgcctc gagatcgacg ccgtctgccg cgaggcgaac attgagatcg
     4801 cctatcccca gcgcgacgtg catctcttca ccgccaagcc gatcgaggtg aaggtccagt
     4861 cggagaaatc cgcctgaaga cggccctgta gccgggatcg ctgatcccgg caatccaaat
     4921 ttcggatccg atccttcaac cggccgggat cagcgatccc ggctacagg
//
LOCUS       DATDAY010000329         2613 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_469821, whole genome shotgun sequence.
ACCESSION   DATDAY010000329 DATDAY010000000
VERSION     DATDAY010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2613)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2613)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2613
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_469821"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..429
                     /locus_tag="VIS74_06535"
     CDS             <1..429
                     /locus_tag="VIS74_06535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792937.1"
                     /translation="IRALPPEYQNNVFQVSADEANPNPATWHLMARHRSNRGVPVYFI
                     VSQGRVISERDSYGLGSIFGSPTAIDLSRVQQDSTDVWTIADHYIAGRGRGLGSVSFR
                     LEQEGRAAVPTWSVWCYDLSGRYAGFLTVLATTGAIISSN"
     gene            429..554
                     /locus_tag="VIS74_06540"
     CDS             429..554
                     /locus_tag="VIS74_06540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792938.1"
                     /translation="MPEPASAQKLIPLDIRSVDALLDAGARQLCKARAEVQLRSA"
     gene            complement(562..1125)
                     /locus_tag="VIS74_06545"
     CDS             complement(562..1125)
                     /locus_tag="VIS74_06545"
                     /inference="COORDINATES: protein motif:HMM:NF015674.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HEY5792939.1"
                     /translation="MSTDSPASPPENPLAKSRGWLIAGGILSLFVGFSAMSFPLLFSV
                     VLVQLLAAFALASGVISLFLAIFGKHSGHRVLEGLMALIRIAAGIALLVCIQSSVLVI
                     TLIFAIFLLVEGLFLVIGAFKLRPHGGWAWTLVSGIAALVLGAMVYSRWPSDSTWVLG
                     LFFGINLIFNGSSLLALGFAAPKAAQA"
     gene            complement(1181..2188)
                     /locus_tag="VIS74_06550"
     CDS             complement(1181..2188)
                     /locus_tag="VIS74_06550"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008720 - D-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0070404 - NADH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HEY5792940.1"
                     /translation="MSAKPIVAVYDTKPYDREYLSSSPGSHHLEWRFLEFRLNAESAR
                     AAEGVQAICVFVNDEVHRTALERLAAFGVRHVALRCAGFNNVDLKAAKELGITVTRVP
                     NYSPHAVAEHTLALILALNRKIYRAYNRVRELNFSLSGLVGFDLHGKTAGVVGTGKIG
                     RIVAEILRGFGMRVLASDPYPNPEWAAGHCIEYVDFPEICRESDLVTLHTPLTKETDH
                     LIRAESIALMKPGVMLVNVSRGRLIHTTALIEGLKNGHIGSVALDVYEEEEGVFFEDL
                     SGDVLPDDELARLLTFPNVIITAHQAFLTREALGEISRVTIENLQLFSRGEPFLEGTP
                     L"
     gene            2364..>2613
                     /locus_tag="VIS74_06555"
     CDS             2364..>2613
                     /locus_tag="VIS74_06555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792941.1"
                     /translation="MGILVSDFDGTLTRFDFFDLVRRRWPVPPEEDPWNQYVAGKITH
                     FEALAEIFRGIRTSEAELEGVVQSMELDPGLAGSIHALE"
BASE COUNT          498 a          774 c          870 g          471 t
ORIGIN      
        1 atccgggcgc tgccgccgga atatcaaaac aacgttttcc aggtttcggc ggacgaggcc
       61 aatcctaacc cggcgacctg gcatctcatg gcccggcacc gctcgaatcg gggcgtgccg
      121 gtttatttca ttgtttcgca gggccgggtg atctccgagc gggactcgta tggcctgggt
      181 tcgattttcg gcagcccgac agccattgat ctaagccggg tgcagcagga ttccactgat
      241 gtctggacga tcgcggatca ctacatcgcc ggccggggcc gcgggctcgg cagcgtgagt
      301 ttccggctgg agcaagaggg gcgcgcggcg gtgccgacct ggtcagtctg gtgctacgat
      361 ttgtccggcc gctacgccgg attcctcacg gtgctggcca cgacgggagc cattatctcc
      421 agcaattgat gccggaaccg gccagcgctc aaaagctcat ccccctggac attcgttcgg
      481 tggatgccct gctggatgcc ggggcgcggc agctgtgcaa ggctcgggcg gaggttcaac
      541 tccgctccgc gtaaccctgt ttcaagcttg ggcggccttc ggggcggcga atccgagggc
      601 gaggagggag ctgccgttga agatcagatt gatgccgaag aacaggccaa ggacccaggt
      661 gctgtcggaa ggccagcggc tgtaaaccat ggcgccaagg acgagcgcgg cgatgccgct
      721 cacgagcgtc caggcccagc cgccatgcgg ccgcaatttg aaggcgccga tcaccagaaa
      781 caggccttca acgaggagaa agatcgcgaa gatcagcgtg atgaccagca cgctggattg
      841 gatgcaaacc agcagcgcga tgcccgcggc aatgcggatc agcgccatca atccttcgag
      901 cacgcgatgc ccggaatgtt tgccaaagat cgccaggaac agcgaaatca cgccgctggc
      961 gagcgcgaag gcggcgagca attgcacgag caccaccgaa aacagcagcg gaaagctcat
     1021 ggcggagaaa ccgacgaaga gcgaaagaat gccgccggcg atcagccagc cgcggctttt
     1081 ggcaaggggg ttttcgggcg gggaagcggg tgaatcggtg ctcatggggt tttctcctta
     1141 gcacgaagcg ggggaaaaac gcgaaacctt aattggggct ttaaagcggc gtgccttcga
     1201 ggaacggctc gcctcggctg aagagctgga gattctcaat cgtcacgcgg gagatttcgc
     1261 ccagcgcctc gcgggtgagg aaggcctggt gcgcggtgat gatgacgttc gggaacgtca
     1321 gcagccgggc gagttcgtcg tcgggcagca cgtcgccgga caagtcctcg aagaacacgc
     1381 cttcctcctc ctcgtaaacg tccagcgcca cgctgccgat gtggccattc tttaatccct
     1441 cgatgagcgc ggtcgtgtga atcaggcgtc cgcggctgac gttgacgagc atgacgccgg
     1501 gcttcatcag cgcgatggat tccgcgcgga tcaaatggtc ggtttccttc gtcagcggcg
     1561 tgtgcagcgt cacgagatcg ctttcccggc agatttcggg aaagtccacg tattcgatgc
     1621 aatgcccggc ggcccattcg ggattggggt agggatcgga ggcgaggacg cgcatgccga
     1681 aaccgcgcag gatttccgcc acgatccggc cgatcttgcc cgttccgacg acgccggcgg
     1741 tcttgccgtg caggtcgaag cccaccaggc cgctgaggga aaaattcagc tcgcggacgc
     1801 gattgtaagc gcggtaaatt ttgcggttca gcgcgaggat gagcgccagc gtgtgctccg
     1861 ccaccgcgtg cggcgaataa ttgggcacgc gggttaccgt gatgccgagt tccttggcgg
     1921 ctttcaggtc cacattgtta aaccccgcgc agcgcagggc cacgtgccgg acgccgaacg
     1981 cggccaggcg ttcgagcgcg gtgcggtgga cctcgtcatt cacaaacacg cagatcgcct
     2041 gcaccccctc cgcggcgcgc gcgctttccg cgttgagccg gaattcgagg aaacgccatt
     2101 cgaggtgatg gcttccgggc gagctgctca ggtattcccg gtcgtaaggc ttcgtgtcgt
     2161 aaacggcgac aatcggtttg gcggacatac ggttccgagg gtcgcgccgc gggaagggaa
     2221 tcgcaagcgc atttgatggg gcattttgtc acttgcgcaa atccgcgcgg gcctgttggc
     2281 cggctcacgg gcagggccgc ggacaaaagc gcgcccttcg accttgacag cccgcggcgc
     2341 gctccctagc caaggattcc gggatgggca ttctcgtttc cgatttcgat ggcacgctca
     2401 cgcgtttcga tttttttgat ctcgttcgcc gccgctggcc cgttccgccg gaggaagatc
     2461 cctggaatca atacgtggcc ggaaaaatca cgcacttcga ggcgctggcg gaaatcttcc
     2521 gcggcatccg gacgagcgag gcggagctgg agggggtggt gcaatccatg gaactcgatc
     2581 ccggcctcgc cggctcgatt cacgcgctcg aag
//
LOCUS       DATDAY010000330         2418 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_834369, whole genome shotgun sequence.
ACCESSION   DATDAY010000330 DATDAY010000000
VERSION     DATDAY010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2418)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2418)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2418
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_834369"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1160
                     /gene="rsmA"
                     /locus_tag="VIS74_06560"
     CDS             <1..1160
                     /gene="rsmA"
                     /locus_tag="VIS74_06560"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEY5792942.1"
                     /translation="GLGSLTQPLLKTGARLTGLELDSDMVRHLRAHFAGADLTILEGD
                     AARADLAAFHTGETVKVIGNLPYSAATAIVLHFCGALGPADRMVFMVQKEVAERLAAA
                     PCCRDYGSLTLAVRRYWDVKLLRILPPEAFHPRPKVHSAVVEFRRKAPAAVPKCDERR
                     LETLIRQGFSERRKQLRKLIGIDREKWESRCAALGCPPTVRAEELSPDQWAALANLLT
                     APANPAPEEIFDVVDENDTPAGQLPRGEVHARRLRHRAVHILIFNAAGRIFLQKRAAW
                     KDINPWVWDSGAAGHLSAGEDYLPAAHREIQEELGVDCAVEKIGKLPPSRITGNEFIE
                     VFTARHEGPFRLERMEIATGAFFDPAQIREWSAKRPRDFSPVFLECLKLIH"
     gene            complement(1255..1866)
                     /locus_tag="VIS74_06565"
     CDS             complement(1255..1866)
                     /locus_tag="VIS74_06565"
                     /inference="COORDINATES: protein motif:HMM:NF016051.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HEY5792943.1"
                     /translation="MKAFVTCGPASEPIDAVRVITNRSTGELGALLAEALAAAQISVD
                     CFRGAGATHPAPAGVAVRTFETNADLWALLDGAGESPDVIFHAAALCDFGGSGTGKIE
                     SRSDELVLRLSPLPKLLPKFRQRFPQARIVGWKFEVEGTGGDARANAERQLRECDSDL
                     CVLNGPAIGAGFEVFSRDRGFIQAVRTKEKLGDFLRDWALSPG"
     gene            complement(1919..>2418)
                     /locus_tag="VIS74_06570"
     CDS             complement(1919..>2418)
                     /locus_tag="VIS74_06570"
                     /inference="COORDINATES: protein motif:HMM:NF019155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HEY5792944.1"
                     /translation="KEAYERKAAWENAEALKGLERYIMLNAVDRLWQEHLYAMDGLRE
                     AVYLRAFAQKDPLVEYKNEAFEMFTELMDNIKGEILSNLFRGTTNLQAFENFLGSLPF
                     NLLHQDPAEPAAPAAPKPAAPVAEPPKPRIELPIKRDLPKAGRNDPCPCGSGKKYKNC
                     CGRLA"
BASE COUNT          418 a          781 c          787 g          432 t
ORIGIN      
        1 ccggcctggg ttcgctcacc cagccgctgc tgaagaccgg cgcgcggctc accggactcg
       61 agctggattc cgacatggtc cggcacctgc gcgcccattt tgccggggcg gatttgacga
      121 tcctcgaagg cgacgccgcc cgggccgacc tggccgcctt ccacacgggc gagaccgtca
      181 aggtcatcgg aaatctgccc tacagcgcgg ccacggcgat cgtcctgcat ttttgcggcg
      241 cgctcggacc ggccgaccgc atggtgttca tggtgcaaaa ggaagtcgcc gaacggctgg
      301 ccgccgcgcc atgctgccgg gactacggct cgctgacgct cgcggtgcgg cgttattggg
      361 acgtgaagct gttgcggatc ctgccgcccg aggcctttca tccgcgtcca aaggtgcatt
      421 ccgccgtggt cgaattccgc cgcaaggcgc cggcggccgt tcccaaatgc gacgaacgcc
      481 ggctggaaac cttgatccgg cagggctttt ccgagcgccg caagcagctg cgcaagctca
      541 tcggcatcga ccgggaaaaa tgggaatcgc ggtgcgccgc gctcggctgc ccgcccaccg
      601 tgcgggccga ggaattgtcg ccggaccaat gggccgcgct ggccaatctc ctgaccgccc
      661 cggccaatcc cgcgcccgag gaaattttcg acgtggtgga cgagaacgac acgccggccg
      721 ggcagctgcc ccgcggcgaa gtgcacgcgc gccggctgcg gcaccgggcg gtgcatattt
      781 tgatcttcaa cgcggcgggg cggatttttt tgcagaagcg cgcggcgtgg aaggacatca
      841 acccctgggt ctgggattcc ggcgcggcgg gccacctgtc cgccggggag gattacctgc
      901 cggccgcgca ccgggaaatc caggaggaac tcggcgtgga ttgcgcggtg gaaaaaatcg
      961 gcaagctgcc gccaagccgg atcaccggaa atgaattcat cgaagttttc accgcgcggc
     1021 acgaagggcc cttccgcctg gaacgaatgg agatcgccac cggggcgttc ttcgacccgg
     1081 cccaaatccg ggaatggagc gcaaagcgcc cgcgggattt cagtccggtg tttttggagt
     1141 gcttgaagct aattcattga ccacagagga cacagagagc acggagacaa accaattggg
     1201 atttttaaag ctttttgctt acctcggtgc tctcggtgtc ctctgtggtg aaaatcatcc
     1261 cgggctcagc gcccagtcgc ggaggaagtc gccgagtttt tccttcgtcc gcacggcctg
     1321 gatgaatccg cggtcccggc tgaagacttc aaaccccgcg ccgatggctg ggccgttgag
     1381 cacgcagagg tcggaatcgc attcccgcag ctggcgctcc gcgttcgcgc gtgcgtcgcc
     1441 gccggttccc tccacttcaa atttccatcc cacgatcctg gcctgaggga aacgctggcg
     1501 gaacttcggc agcaatttgg gcagcgggga aagcctgagc acgagctcgt ccgagcggct
     1561 ctcgattttg cccgtgccgc tcccgccaaa atcacagagc gccgcggcgt gaaaaatcac
     1621 gtccggggat tcgccggcgc cgtcgagcag cgcccacaag tccgcattgg tttcaaaggt
     1681 ccgcaccgcc acgcccgccg gcgcgggatg ggtggcgccg gctccccgaa agcaatccac
     1741 ggaaatctgc gcggcggcca gggcctccgc cagcaaggcg cctaactcac ccgtggagcg
     1801 gttcgtaatg acccgaaccg catcaatcgg ctcggacgcc ggtccgcagg tgacgaaggc
     1861 tttcatggtt tattcagctg gagcggcggt cacaggccgc cgctccaaat aaaaaacgtc
     1921 acgccaggcg gccgcagcag ttcttgtatt tcttgccgct gccgcagggg cacggatcgt
     1981 tccggccggc cttgggcagg tcgcgtttga tcggcagttc gatcctcggc ttcggcggct
     2041 ccgccaccgg cgcggcgggt ttgggcgcgg ccggagcggc cggctccgcg gggtcctggt
     2101 gcagcaggtt gaacggcagg ctgccgagga agttttcgaa ggcctggaga ttggtcgtgc
     2161 cgcggaagag attgctgaga atctcgccct tgatgttgtc catcagctcg gtgaacattt
     2221 cgaacgcctc gttcttgtat tccaccagcg gatccttctg ggcgaaggcg cggagataaa
     2281 cggcctcgcg caatccatcc atcgcgtaaa ggtgctcctg ccagaggcgg tccacggcgt
     2341 tgagcatgat gtagcgctcc agccccttga gcgcctcggc gttttcccac gcggccttgc
     2401 gctcgtaggc ttccttga
//
LOCUS       DATDAY010000331         3043 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_1323884, whole genome shotgun sequence.
ACCESSION   DATDAY010000331 DATDAY010000000
VERSION     DATDAY010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 3043)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3043)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3043
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1323884"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            110..775
                     /locus_tag="VIS74_06575"
     CDS             110..775
                     /locus_tag="VIS74_06575"
                     /inference="COORDINATES: protein motif:HMM:NF017534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEY5792945.1"
                     /translation="MADSTAPDFWNERYQCEKTPWDFHGVPAALTQFLDAAKPGRVLL
                     PGCGSGYEIAAFLDAGWEAWGLDFSQAAVRRARALLGTRADRVLFGDFFNYEFGQPFD
                     LIYERTFLCALPPALWESYASRIKNLLIPGGRLAGFFFYGPEAAESPPYPLSRADAER
                     LFPPGLELIEDQPAADSLPLFAGKERWQQWKKRSAGPPAPPSAERCPAGIVPPPSAQG
                     PRI"
     gene            857..1099
                     /locus_tag="VIS74_06580"
                     /pseudo
     CDS             857..1099
                     /locus_tag="VIS74_06580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012599651.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            complement(1255..1488)
                     /locus_tag="VIS74_06585"
     CDS             complement(1255..1488)
                     /locus_tag="VIS74_06585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792946.1"
                     /translation="MFFELQQPGYLHVLINHLPIIGTVAGLFGLIVALLLRSRIALVP
                     ALAIVLLSGISARPVYITGSNAYRPLMKISDDQ"
     gene            complement(1555..1818)
                     /locus_tag="VIS74_06590"
     CDS             complement(1555..1818)
                     /locus_tag="VIS74_06590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792947.1"
                     /translation="MIIALPSVKAADDKTAKPYPLQTCFISGDKLGEMGKPVVFVYEG
                     QEIKLCCKDCKKQFDKDPAAAMKKFNEAVKSKPSGTGHSMNHM"
     gene            complement(1833..2147)
                     /locus_tag="VIS74_06595"
     CDS             complement(1833..2147)
                     /locus_tag="VIS74_06595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792948.1"
                     /translation="MNRINRGEPDGGEVAAGIAAELKNALRNYTDAKGKGHPGTALLA
                     AYPSLATRFGSTSRNGLSARKKGSKNLRDFSGALVCLNKKHMAFISPESNNQNQIMRL
                     TA"
     gene            2286..2702
                     /locus_tag="VIS74_06600"
     CDS             2286..2702
                     /locus_tag="VIS74_06600"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEY5792949.1"
                     /translation="MNASDFEPCRGCACLNLRKASRSVTQHYEAHFRGSELRATQFTL
                     LAVLVQTGPMPMTQLARMLGMERTTLTRNLALLERKGFIKLSGDDDARVRLVEITPSG
                     KTAAARGLVIWKRAQAGVGAVLAGLDLDWLRAAAIP"
     gene            2731..>3043
                     /locus_tag="VIS74_06605"
     CDS             2731..>3043
                     /locus_tag="VIS74_06605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792950.1"
                     /translation="MNSSILFDLIGAVELTASAAITVALLSIGFGRSWTERARLASAL
                     GAWFVLVVVFAATEIFSSRPGSLGVGGVGISVAVPLLILCVAVFRVPSLRSALEKVPL
                     AL"
BASE COUNT          578 a          847 c          899 g          719 t
ORIGIN      
        1 tattgggtgt gggcttggtt atccgcagtg ccgccaagaa tatatcgaag ccccggcttg
       61 cacaagcgtt atgcggatgg ccggacttgt ggtaatctgc cggcaagata tggctgattc
      121 gaccgcgccg gatttttgga acgagcgtta tcaatgcgaa aaaacacctt gggactttca
      181 cggcgtgccg gcggctctga cgcaattctt ggatgccgcc aaaccgggaa gagtcctgct
      241 gccgggctgt gggtcgggtt atgaaatcgc ggcctttctc gatgcgggct gggaggcatg
      301 gggtctcgac ttttcccaag ccgccgtgcg gcgggcccga gcccttctcg gcacccgggc
      361 ggatcgggtg ttgtttgggg attttttcaa ttatgaattc ggccaaccgt tcgacttgat
      421 ctatgagcgg acgtttttgt gcgcgctgcc gcccgcgctt tgggaatcgt atgcttcgag
      481 gatcaaaaat ctcctcatcc ccggaggccg gctcgcggga ttcttctttt acggtccgga
      541 ggcagccgag tcgccgccgt atcccctgag ccgggcggat gccgaaagac tatttcctcc
      601 cggtttggaa ttgatcgagg atcaaccggc ggccgattcc ctgccgctct tcgccggcaa
      661 ggagcgctgg cagcaatgga aaaagcgatc ggccggcccg cccgctccgc catccgctga
      721 acgttgtccc gccggcatcg tgcctcctcc ttccgcgcag ggcccacgca tctaaatttc
      781 gctgaaggtt ttgtctattt cgtgacaccg agataagggc tgtacaaatt ttcaacagtt
      841 cagggataag cttgggatgc aaccgcctga gcgcctgaac ctgatcacga tcattcgagc
      901 cattgaggac ccgcgagtgg agcgaacgcg gcggcacgag cttgaagata tcctggtgat
      961 tgcgatttgc gcgttgcggt gtggtgcgga gagtttcgag gacatggaag cctttgggga
     1021 atccaaagaa gagtggttgg gaacattttt ggaactgccc cacggcatcc ccagtcacga
     1081 cacgtttggc cgggtttttt gcggcgatgg atcccgaaaa gtttttagat aaatcggcga
     1141 aagatggttc cgttctcaat cgtcaattcc gcttgttcgc ttagacttac atagttcacc
     1201 caattctgcc gaaattcgac aatgacctgt ttttttgcct tgatggatat gactttactg
     1261 atcgtccgaa attttcataa ggggccgata ggcattgctt cccgtgatat aaaccggccg
     1321 ggcggagatg ccagaaagaa ggacaatggc gagagccggc acgagggcga tgcggcttct
     1381 caatagcaac gcgacaatca acccaaaaag cccggctacc gtgccaatga tcggcaggtg
     1441 attaatgagg acgtgaagat agcccggctg ttgaagttca aaaaacacaa gaggggcgga
     1501 gacgcggctt caggccacac ctccgctggt tgaagttggc gcgcttctac gatctcacat
     1561 gtggttcatc gaatgccccg tgccggacgg cttcgatttg acggcttcat tgaatttctt
     1621 catggcggcg gcggggtcct tgtcgaactg ctttttgcag tccttgcagc agagtttgat
     1681 ttcctgcccc tcgtaaacga agacgaccgg tttgcccatt tcaccgagct tgtcgccaga
     1741 gatgaagcaa gtttggagcg gatacggttt ggcagttttg tcatctgcgg ctttgacgct
     1801 cggcagagca atgatcatcg cgaaagccgc gatcaggcag tcagtctcat gatttggttt
     1861 tggttgttgg attccggtga aatgaaagcc atgtgcttct tattgagaca aacgagagca
     1921 cctgaaaaat ccctcaaatt ttttgagcct tttttgcggg cgctcaatcc attgcggctc
     1981 gttgagccga agcgcgttgc caatgacgga tacgctgcta aaagcgcagt tcccgggtgg
     2041 cccttgccct tggcgtcggt gtagttccgc agcgcgttct tcagctcggc ggcgatgccg
     2101 gccgcaacct cgccgccgtc cggctccccg cggttgatcc gattcacggg cctgtttgcc
     2161 aggcccgttt ttctcgtagc tttacaggtc aatcgccaca tcccggtcaa ttgccgcatc
     2221 tcatactttg atcgaaccgc aatgtgccct tgacgcgccg cctaaatgcg tgtatctaca
     2281 ccattatgaa tgcatcggac ttcgaacctt gccgaggatg cgcgtgcctt aacctgcgca
     2341 aggccagccg atccgtgacc caacactacg aggcccattt tcggggcagc gagctgcgcg
     2401 cgacacagtt cacactgctg gcggttctgg tgcagaccgg tccgatgccg atgacgcagc
     2461 ttgccaggat gctgggcatg gaacgcacca ccctgacccg gaatctcgcc ctgctggagc
     2521 ggaagggatt catcaaactt tctggcgatg acgatgcccg tgtccggctg gtggagatta
     2581 ctcccagcgg caaaacggca gcggcccgtg gcctcgtgat ctggaaacgg gcgcaggcgg
     2641 gcgtcggggc agtcctggcc ggtctcgatc tggactggct tcgcgcagca gcgattccct
     2701 gattttttta accaacaacg tgtatctaca atgaactcat ccatcctgtt cgatctcatc
     2761 ggcgccgtcg aattgacggc atctgcggcc atcactgttg cgttgctctc cattggcttc
     2821 ggccgaagct ggacggagcg ggcgcggctc gcttccgccc tgggggcgtg gttcgtcctg
     2881 gtcgtcgtct ttgcggcgac ggaaatattc agctcccggc ccggatccct cggagtaggc
     2941 ggagtgggga tctccgtggc ggtgccattg ctgatcctct gcgtggctgt ctttcgcgtg
     3001 ccttcgctcc gctccgcgct ggagaaggtg ccgctggctc tgc
//
LOCUS       DATDAY010000332         4786 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_1881286, whole genome shotgun sequence.
ACCESSION   DATDAY010000332 DATDAY010000000
VERSION     DATDAY010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 4786)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4786)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4786
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1881286"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..284)
                     /locus_tag="VIS74_06610"
     CDS             complement(<1..284)
                     /locus_tag="VIS74_06610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792951.1"
                     /translation="MAKLNGILLAILILLAVAVTVLSTGAARAVQSTFQSFISPFLNA
                     GKFAQEQIGSVGKGLKTLDQLDAENRELIVENRELRATVQILHDLESENN"
     gene            complement(337..1383)
                     /locus_tag="VIS74_06615"
     CDS             complement(337..1383)
                     /locus_tag="VIS74_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002621977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HEY5792952.1"
                     /translation="MFNSLLGFFSNDIGIDLGTANTLVYVKDHGIVLREPSVVAVQQG
                     TNKVLAVGDEAKRMLGRTPGNIVAIRPLKDGVIADFEITEAMLRHFISKVHNRRRMVR
                     PRVVIAVPSGITEVEKRAVKDSATHAGAREVYLIEEPMAAAIGVGVPVQDAAGNMIVD
                     IGGGTTEVALISLSGIVFSRSVRVAGDELDEAIAQYMKRAYNLMIGERTAEDIKIKIG
                     SAYKLEKETTIEVKGRDMVAGLPKTITISSQEVREALLEPISTIVDSVRIALERCPPE
                     LSADLVDRGLVLAGGGALLRGLDRLLAEETGLPVHVAEDPLSAVAEGTGKALQEIKFL
                     RAVASTDKAGTSYY"
     gene            complement(1523..2626)
                     /gene="leuB"
                     /locus_tag="VIS74_06620"
     CDS             complement(1523..2626)
                     /gene="leuB"
                     /locus_tag="VIS74_06620"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HEY5792953.1"
                     /translation="MSQEKSLKIAVLAGDGIGPEVMAEALKVLQRTADRFRFSLDLKE
                     YLVGGIAIDEAGKALPEETLKGCEAADAILFGSVGGSKWESLPPKEQPERAALLPLRK
                     HFGLFANLRPAVCLPELAHASPLHPKSVEGGFDVLCVRELTGGLYFGEPKYIKKEGGE
                     EFAVDTMVYWESEIVRIARVAFEAARKRRKRVTSIDKANVLQNGLLWRRVVTEVAKDF
                     PDVALNHLYVDNAAMQLIKNPKGFDVLLAENLFGDIRSDEMAMIAGSLGMLPSASLGE
                     SMGASRRFGMYEPSGGTAPDIAGKGIANPIAQILSAGMMLRYSFGLEDAAAAIEKAVH
                     QAIQNGLRTRDILTPEFQPVSTAEMGGAISRLI"
     gene            complement(2660..3304)
                     /locus_tag="VIS74_06625"
     CDS             complement(2660..3304)
                     /locus_tag="VIS74_06625"
                     /inference="COORDINATES: protein motif:HMM:NF012900.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HEY5792954.1"
                     /translation="MRERFFEPGPLRVKICGLTREPDARRSGELGADALGFNFWPQSK
                     RYLDPVEAVPWIRALPGDAARVAVVVNPEPELLAGLLAAGCFEAIQFHGNESPEFCRR
                     HGGPRWIKALAVRPENRGQPGAFPTADVLLDAPSAGFGGSGEAADWSLAAEWVKSHPE
                     KRFILAGGLTAANVFEAAGTVRPHAVDVASGVESGPGIKDLDRVRDFIAQARAA"
     gene            complement(3301..3717)
                     /locus_tag="VIS74_06630"
     CDS             complement(3301..3717)
                     /locus_tag="VIS74_06630"
                     /inference="COORDINATES: protein motif:HMM:NF015953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEY5792955.1"
                     /translation="MAAFLVRWLITAFAVMVASFLLRGISYSDSLAVLLGASLLLGII
                     NALIRPVLLILSLPFILVTMGIFIFVINALLLLLVSKMIPGFQVDGFWSAFFGSIIIS
                     IVSWLLSAFFRGSDGKVYSITHHGRLKRANARVVGE"
     gene            3824..4288
                     /locus_tag="VIS74_06635"
     CDS             3824..4288
                     /locus_tag="VIS74_06635"
                     /inference="COORDINATES: protein motif:HMM:NF022603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2934 domain-containing protein"
                     /protein_id="HEY5792956.1"
                     /translation="MDKKPITEKSSLSAAPKPKITPAKPAPAAKAPAPAKPAPAPATP
                     AAKAPAPVRPAPAPRPPVARPAPAAKAPVPAKPAPAPAAPAAKPAPAAKAPAPAKPAI
                     KAAIISISTDDIALRAYFIAERRQNLGWDGSPESDWLEAKRQLEEESTSKGL"
     gene            4537..>4786
                     /locus_tag="VIS74_06640"
     CDS             4537..>4786
                     /locus_tag="VIS74_06640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEY5792957.1"
                     /translation="MKSKTSAKASAKSAPERGQLMNGADILISCLEREGVEVIFAYPG
                     GASMPLHQSLTKSTKIRTILPRHEQGGVFAAEGYARVTG"
BASE COUNT          940 a         1648 c         1338 g          860 t
ORIGIN      
        1 tggttgtttt cggattccag atcgtgcagg atctggaccg tggcccgcag ctcccggttt
       61 tccacgatca attcccggtt ttccgcgtcc agttgatcga gggttttcag gcccttcccc
      121 accgagccga tttgctcctg cgcgaatttg cccgcattca aaaacggcga gatgaatgac
      181 tggaaagtcg attggaccgc cctcgccgcc cccgtgctca agacggtcac cgccaccgcg
      241 agcaggatga gtatggcgag taaaatgccg ttaagttttg ccattgcact ttaacttttc
      301 gctgtccgat accgcgccgg cgcatcgcgg ttccgcctaa taataactgg ttccggcctt
      361 gtccgtcgaa gcgaccgccc ggagaaactt gatttcctga agcgccttgc ccgtgccttc
      421 ggccaccgcg ctgagcggat cttccgcgac atgcaccggc aacccggtct cctcggccag
      481 caggcggtcg agcccccgca gcaacgcgcc gcctccggcc agcaccaacc cgcggtccac
      541 cagatccgcg gaaagctcag gcgggcagcg ctccagcgcg atgcgcacgg agtccacgat
      601 ggtcgaaatg ggttccagca acgcctcgcg aacctcctgg gaggaaatgg tgatcgtctt
      661 cggcaatccg gccaccatgt cccgcccctt gacctcgatg gtggtttcct tttccagctt
      721 gtaggccgat ccgatcttga tcttgatgtc ctccgccgtg cgctccccga tcatgagatt
      781 gtaggcgcgc ttcatgtatt gcgcgatcgc ctcatcgagc tcatcccccg ccacccgcac
      841 gctgcggctg aaaacgatgc cggagagcga aatgagcgcc acctccgtgg tgccgccgcc
      901 gatgtccacg atcatgttgc ccgccgcatc ctgcaccggc acgccgacgc cgatggccgc
      961 cgccatgggt tcctcgatca aatacacctc ccgcgcgccg gcgtgggtcg cggaatcctt
     1021 caccgcgcgt ttttccactt ccgtaatgcc gctcggcacc gcgatcacca cccgcggacg
     1081 caccatgcgg cggcggttat gcaccttgct gatgaagtgg cgcagcatcg cttcggtgat
     1141 ctcgaaatcg gcgatgacgc catccttcaa cggccggatc gcgacaatgt tgcccggggt
     1201 gcggcccagc atgcgtttgg cctcgtcccc gacggcaagc accttgtttg tcccctgctg
     1261 cacggccacc accgagggct cgcggagcac gatgccatga tcttttacat aaacgagggt
     1321 attcgccgtg ccgagatcaa tgccgatgtc attcgagaaa aatccaagta aactgttgaa
     1381 cattcaaata gctttgggtg aaagtcttag gtagcatcag acccgtcagc caaggcagcg
     1441 ggttccacac tcagagttcg cagcctctct ccctccccca ttggagtcaa gggcggattt
     1501 tggcaaacta aaccggattt gctcagatta accgcgagat ggcccctccc atttccgccg
     1561 tgctcaccgg ctgaaattcg ggggtcagaa tgtccctcgt tcgcaggccg ttttggatcg
     1621 cctgatggac ggctttttcg atggccgccg ccgcgtcctc caagccgaag gaataccgca
     1681 acatcatgcc cgccgagaga atctgcgcga tcgggttggc gattcccttg ccggcgatgt
     1741 ccggcgcggt gccgccgctg ggttcataca tgccaaagcg gcgggaggcg cccatgctct
     1801 cgccgagact cgcgctgggc agcatgccga gcgaaccggc gatcatggcc atctcgtcgc
     1861 tccggatgtc gccaaagaga ttttcggcca gcagcacatc gaagcccttg gggtttttga
     1921 tgagctgcat cgccgcattg tccacgtaga gatgattgag cgcgacatcg ggaaaatcct
     1981 tcgccacctc ggtcaccacg cggcgccaga gcaagccatt ttgcagcaca ttggccttgt
     2041 cgatggaagt cacccgcttg cgccgcttcc gcgcggcctc gaaggcgacc cgcgcgatcc
     2101 gaacgatctc gctctcccag taaaccatcg tgtccaccgc aaattcctcg ccgccttcct
     2161 ttttaatgta tttcggctcg ccgaaataca gcccgcccgt cagctcccgg acgcagagga
     2221 catcaaagcc gccttccacc gatttgggat gcagcggcga cgcgtgcgcc agctcgggca
     2281 ggcagaccgc gggacggaga ttggcaaaga ggccgaaatg tttgcgcagg ggcagcagcg
     2341 ccgcgcgctc cggctgctcc ttgggcggca ggctttccca tttggaccct cccaccgaac
     2401 caaacaggat cgcatccgcc gcttcgcagc ctttcagcgt ctcctccggc agggccttgc
     2461 ccgcctcgtc aatggcgatc ccgccgacca gatattcctt caaatccagt gagaagcgga
     2521 atcgatccgc cgtgcgctgg aggaccttga gggcttccgc catgacttcc gggccgattc
     2581 cgtcgcccgc cagcacggca attttcaaac ttttttcctg actcatgccc ggccatctaa
     2641 gccgagccat cgtcaggaat caagcggcgc gtgcctgcgc gatgaaatcc cggacgcggt
     2701 ccaggtcctt gattcccggg cccgactcca ctccgctggc cacgtccacc gcgtgcggcc
     2761 gcacggttcc cgccgcctca aacacattgg ccgccgtcag cccgccggcg agaatgaacc
     2821 gcttttccgg atgggatttc acccattccg cggccagcga ccaatccgcc gcctcgcccg
     2881 agccgccgaa acccgcgctg ggcgcgtcga gcagcacatc cgccgtggga aacgcgccgg
     2941 gctgtccccg gttctccggc cgcaccgcga gcgccttgat ccaccgcggc ccgccatggc
     3001 gccggcagaa ttccggcgat tcgttgccgt gaaactgaat ggcctcaaaa caccccgccg
     3061 cgagcagccc ggccagcagt tccggctccg gattcaccac gaccgccacg cgggcggcgt
     3121 cccccggcaa cgcgcgaatc cacggcaccg cttccaccgg atcgagatac cgtttcgact
     3181 gcggccagaa attaaagccc agcgcgtccg ccccgagctc gccgctccgc cgcgcgtccg
     3241 gctcccgcgt gaggccgcag attttgaccc gcagcggacc gggttcgaaa aaacgctccc
     3301 tcactcgccc accacccgcg cattcgcccg cttcaggcgg ccgtgatgcg tgatgctgta
     3361 aaccttgccg tcgctgcccc ggaaaaacgc gctcaagagc cagctcacga tgctgatgat
     3421 gatcgatccg aaaaacgcgc tccagaatcc gtccacctga aatccgggaa tcatcttgga
     3481 gaccagcagc agcagcagcg cgttgatgac aaaaatgaag atgcccatgg tcaccaaaat
     3541 gaacggcagg ctgagaatca gcagcaccgg ccggatgagg gcgttgatga tgcccaaaag
     3601 gagcgaggcg cccagcaaaa ccgccagcga atcgctgtaa ctgatgccgc gcaggagaaa
     3661 ggaggcgacc atgaccgcaa aagcggtaat taaccaacga acgagaaatg cggccatgcc
     3721 ggaattctaa aatttcacgg ggaatagaga agatttattt tgattcgacc ggcagattcc
     3781 ggcaacctca ggttccatag cattcaactc ggcaaaccca tctatggaca agaaaccaat
     3841 cacggaaaaa agcagtttgt cggcggcccc caagccgaag atcactccgg ccaaacccgc
     3901 gcccgccgct aaagcgcccg cacccgccaa acccgctccg gcgccggcga cgccagccgc
     3961 aaaagcgccc gcgcccgtca gacccgcacc ggcacccagg ccgccagtcg ccagaccggc
     4021 tcccgccgcc aaagcgccgg ttcccgccaa gcccgcgccg gctccggcgg cgccagcggc
     4081 caaacccgct ccggccgcca aggcccccgc gcccgccaag cctgccatca aggcggcgat
     4141 tatctccatc agcaccgatg acatcgccct gcgcgcttat ttcatcgccg aacgccgcca
     4201 gaatctgggg tgggatggca gcccggagag cgactggctg gaagccaagc ggcagctcga
     4261 agaggaatcc accagcaagg gattgtagcc ggcctcaagg tggcgggcgg ctcacccgag
     4321 ccgccgtgct tcccaaaatt cgaatcgcgg acgcgaaggc tgatttaatc cgccgatttc
     4381 accgatggac gcggatttta taaaacctcg gttttatccg cgaaaaccgg cgtcctgccg
     4441 ctgtgggatg gataatcccc ctcctcggtt taatcagcgc ttccccggtt attgccttgc
     4501 catcggccgg gataattggc aatttcaagg gttcctatga aatccaagac ttcggccaaa
     4561 gcttccgcca aatccgctcc cgagcggggt cagctcatga acggcgcgga tatcctcatc
     4621 agctgcctgg agcgtgaggg ggtcgaagtc atttttgcgt atcccggcgg cgcctcgatg
     4681 ccgctgcatc agtcgctcac caaatccacg aaaatccgca ccattcttcc gcgccatgag
     4741 cagggtgggg tgtttgccgc cgagggctac gcccgggtga ccggca
//
LOCUS       DATDAY010000333         4609 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_1881396, whole genome shotgun sequence.
ACCESSION   DATDAY010000333 DATDAY010000000
VERSION     DATDAY010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 4609)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4609)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4609
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1881396"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..813)
                     /gene="murF"
                     /locus_tag="VIS74_06645"
     CDS             complement(<1..813)
                     /gene="murF"
                     /locus_tag="VIS74_06645"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEY5792958.1"
                     /translation="MDPLLLTEIAQMSGGRLENGETGLTAAHLSKDTRSLRPGDLYLA
                     LRGENFDGNQFIEAARRAGAAGALVDDASLVERFPNFPLIVASDSLIALQRRAAGWRE
                     RLDLRVVGLSGSSGKTSTKDFTAAVLRERFLVTTTAGNLNNHIGLPLSILAAASRDQF
                     AVWEMGMNHPGEIEPLARLARPDIAILTGIGVAHIENLGSREAIAAEKGALVRLTSPH
                     GTVILPAEDDFTESLARFAPCEVVTVGLRAGQIRAEDIAFDSGSSRFTLLHGS"
     gene            complement(813..2291)
                     /locus_tag="VIS74_06650"
     CDS             complement(813..2291)
                     /locus_tag="VIS74_06650"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF001126.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HEY5792959.1"
                     /translation="MTLSELTTGIPGVQLAAETNPEITGLTYDSRQVSPGDLFFALPG
                     AKTNGVEFAAEAAQKGAAAVLTQRELPLVPCPVILAQNAREAMARIASRFYGEPSRQL
                     KVAGVTGTNGKTTTAYLIKHLCDRAALRCGLIGTIHYAVGDEILPAARTTPESADIQK
                     MFAGMRAAGGRACAMEVSSHALVQHRADAIQFTAAVFTNLTQDHLDYHETMDAYYAAK
                     AHLFELLQAGGSKKAKAVINADDRFGHRLLEQFGGKLPIVTYGLGATAQFRASNVKMD
                     AHGATYRLEAKGREYLVRLPLIGMFNVYNSLAALAAANALGVELRAAVAALAQAPQVP
                     GRLERVPAKRSFQVFVDYAHTDDALRNVLRTLKDLKPARLITVFGCGGDRDKTKRPKM
                     AAAAEEYSDYVVATSDNPRGEDPQAILADIRKGLKATWNECIEDREAAIRRAVELAGP
                     GDIVLIAGKGHENYQEFANGRRIPFDDASVAGRAIHGKAVQS"
     gene            complement(2288..4048)
                     /locus_tag="VIS74_06655"
     CDS             complement(2288..4048)
                     /locus_tag="VIS74_06655"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEY5792960.1"
                     /translation="MRCASRKRISIAIIGVGAVYSLFCARLVDLQVFKHEEYAAIAAQ
                     KNSIRQVIQARRGLICDRSGEILAANIPTRTVVADGSHIQDPARLAALAAPWLEMDEA
                     DLAQKLKTGRKYLVLKKGVDEEKAVALIKQMQAAGVRGLYFEPTSARVYPNGPMLAHV
                     IGFLDHENQGIQGIEKTMENYLHGQDGFRHIERDRTGREIVVYRGQEQAPRNGAQVEL
                     AIDMALQAIVEEEIDNAFQELKPRSVVAILMRPKTGEILAMANRPSFDLNEVSKARPE
                     EMKNRAILDIVEPGSTFKIVAVGAAIDNGTVKPDTLIYCENGHFAYGGRTLKDHHGYG
                     NLSVHDVLMKSSNIGSAKIAMLMGNQRYYEYVKRYGFGERSGINLPGEVTGIVHPPQR
                     WSKLSITRIPMGHEVAVTPLQIVMAMGAIANGGKLMRPQIVRAIKSERGEELASFPPE
                     VVRQVVSPEAAHQVNLALTDVVSPKGTAYLAKVEGFQVAGKTGTAQRVDPRGGYTPGK
                     YVVSFCGYMPAEAPEFVGLVLVDDPQTSPGMNYGGLVAAPIFSRIAEKAARYLDLQPT
                     VTLPSEIAQLGRPLERRATP"
     gene            complement(4045..4425)
                     /locus_tag="VIS74_06660"
     CDS             complement(4045..4425)
                     /locus_tag="VIS74_06660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792961.1"
                     /translation="MSNLRRNPTTNRVEIKPVVFWLLAAFFAGMSGLAFVHIKNQQHA
                     IGQQTRDAERQTAELRAANQVLAAHITNLTSRASLQRRLAEGFIAMIPIKDHAITRLG
                     QPVLNGDSLILGTATASANSRFSP"
BASE COUNT          724 a         1408 c         1608 g          869 t
ORIGIN      
        1 ggaaccgtgc aggagcgtga aacgggagct gccggagtcg aaggcgatgt cctccgcgcg
       61 aatctgcccg gcccgcaatc ccaccgtgac cacctcgcag ggcgcgaaac gggcgaggga
      121 ttccgtgaaa tcatcctcgg cgggcaggat caccgttccg tgcggcgagg tcagccggac
      181 caaggcgcct ttctcggcgg cgatcgcctc gcggctgccg aggttctcga tgtgcgcgac
      241 gccgatgccg gtgaggatgg cgatatccgg ccgggcgaga cgggcgagcg gctcgatctc
      301 gccgggatgg ttcatgccca tttcccagac ggcgaattga tcccgcgagg ccgccgcgag
      361 gatggagagc ggcaggccga tgtggttgtt gagatttccc gccgtggtgg tcacgaggaa
      421 ccgctcgcgc agcacggcgg cggtgaagtc cttcgtgctc gtcttgccgc tgctgccgct
      481 cagtccgacc acgcgcaggt cgagccgctc gcgccagccg gcggcgcgcc gctgcagcgc
      541 gatcaatgag tcgctggcga cgatgagcgg aaaattcgga aaccgttcga cgagcgaggc
      601 gtcatccacg agggcgccgg cggctccggc ccgccgcgcg gcttcgatga attggttgcc
      661 gtcgaaattt tccccgcgca gcgcgaggta taggtcgccg ggccgcaggc tgcgcgtgtc
      721 cttgctgaga tgcgcggcgg tcaggccggt ctccccgttt tcgaggcggc cgccgctcat
      781 ttgggcgatt tcggtgagga ggagagggtc cattacgact gcaccgcctt tccgtgaatg
      841 gcgcgtccgg ccaccgaggc gtcgtcgaag ggaatgcggc ggccattggc gaattcctgg
      901 taattttcgt gccccttgcc ggcgatcagg acgatgtcgc ccggcccggc gagctccacg
      961 gcgcggcgga tcgcggcttc ccggtcctcg atgcattcat tccacgtggc cttcaacccc
     1021 ttgcggatgt cggcgagaat ggcctgcgga tcctcgccgc gcggattgtc ggaggtggcg
     1081 acgacatagt ccgaatattc ctcggcggcg gcggccatct tcgggcgctt ggttttgtcg
     1141 cgatccccgc cgcagccgaa gacggtgatc aaccgcgcgg gcttcagatc cttgagcgtg
     1201 cgcaggacat tgcggagcgc gtcgtcggtg tgggcgtaat ccacgaagac ctggaaactc
     1261 cgcttggcgg gcacgcgctc gaggcgcccg ggcacctgcg gcgcctgcgc cagggcggcg
     1321 acggcggcgc gcagttcgac gcccagcgca ttggcggcgg ccagcgcggc gagggagtta
     1381 tagacgttga acattccgat gagcggaagc cgcacgaggt attcgcgccc cttggcttcg
     1441 agccggtagg tggcgccgtg ggcgtccatt ttgacattgc tcgcgcggaa ctgggcggtg
     1501 gcgccgaggc cgtaggtgac gatcggcagc ttgccgccaa attgttcgag caggcggtgg
     1561 ccgaagcggt cgtcggcatt gatgaccgcc ttcgcctttt tggagccgcc cgcctgcagc
     1621 agctcaaaga gatgggcctt cgccgcgtag taggcgtcca tcgtttcgtg ataatcgaga
     1681 tggtcctgcg tgaggttcgt gaagacggcg gcggtgaatt gaatggcgtc cgcgcggtgc
     1741 tggaccagcg cgtggctgga aacctccatg gcgcaggcgc gcccgcccgc ggcccgcatg
     1801 ccggcgaaca ttttctggat gtcggcggat tccggagtcg tgcgcgcggc gggcaggatc
     1861 tcgtcgccca cggcgtaatg aatggtcccg atcaatccgc agcgcagcgc cgcccgatcg
     1921 cagaggtgct tgatgagata agcggtggtg gttttgccat tggtgccggt cacgcccgcg
     1981 accttgagct ggcgggaggg ctcgccgtaa aaccggctgg cgatccgcgc catcgcctcc
     2041 cgggcgttct gggcgagaat caccgggcag gggacaaggg gcagctcccg ctgcgtgagc
     2101 acggcggcgg cgcctttttg ggcggcctcg gcggcgaatt ccaccccgtt cgtcttcgcc
     2161 ccgggcaggg cgaaaaacag atcgccggga ctgacctggc gcgaatcgta ggtgagtccg
     2221 gtgatctcgg ggttggtttc ggcggcgagt tgcactccgg gaattccggt cgtgagctcg
     2281 gagagggtca cggggtcgcc ctcctttcca ggggccggcc caattgcgcg atttccgaag
     2341 gcagcgtgac ggtgggttgc aaatcgaggt aacgcgcggc tttttccgcg atgcgtgaaa
     2401 aaatcggcgc ggcgacgagg ccgccgtaat tcattccggg gctggtctgc ggatcatcca
     2461 cgagcacgag gccgacgaat tccggagctt cggcgggcat gtagccgcag aacgaaacca
     2521 cgtatttgcc cggcgtataa ccgcccctgg ggtccacgcg ctgcgcggtg ccggtcttgc
     2581 ccgcgacctg gaaaccctcc accttggcga gatacgcggt ccccttcggg ctgaccacgt
     2641 cggtgagcgc cagattgacc tgatgggcgg cttcgggaga aaccacctgc cggaccacct
     2701 cgggcgggaa gctcgccaat tcctctccgc gctcggattt gatcgcacga acgatctggg
     2761 gtctcatcaa tttgccgcca ttggcgatcg ctcccatggc catgacgatc tgcagcggcg
     2821 tgacggccac ctcgtgcccc atgggaatgc gggtaatgga gagcttgctc cagcgctgcg
     2881 gcgggtgcac gatgccggtg acctcgccgg gaaggttgat gcccgagcgc tcgccgaacc
     2941 cgtagcgctt gacgtattcg taataacgct ggttgcccat gagcatggcg atcttggccg
     3001 agccgatgtt ggaggacttc atgagcacgt cgtgcacgct caggttgccg tagccgtggt
     3061 gatccttcaa ggtgcggcca ccataggcga aatggccgtt ttcgcaataa atcagggtgt
     3121 ccggcttgac ggtgccatta tcgattgcgg ccccgacggc cacgatcttg aaggtcgaac
     3181 ccggttcgac gatgtccagg atggcgcgat tcttcatctc ctcgggcctg gccttgctga
     3241 cctcgttgag gtcgaaggac gggcggttgg ccatggccag aatttcgccg gtcttcgggc
     3301 gcatcaaaat cgcgaccacg ctccgcggct tgagctcctg gaaggcgttg tcgatttcct
     3361 cctccacgat cgcctgcagg gccatgtcga tggccagctc gacctgcgcg ccgttccgcg
     3421 gggcctgctc ctggccgcga taaaccacga tctccctgcc ggtccggtcc cgctcgatgt
     3481 ggcggaagcc atcctgcccg tgcaggtaat tctccatcgt tttctcgatg ccctgaatgc
     3541 cctggttttc gtggtcgagg aatccgatca cgtgggcgag catcgggcca ttgggataaa
     3601 cgcgggccga ggtcggctca aaatacaggc cgcgcacgcc ggccgcctgc atctgcttga
     3661 tcagggcgac ggccttttcc tcgtccacgc cctttttgag gacgaggtat ttgcggccgg
     3721 tcttgagctt ctgcgccagg tcggcttcgt ccatttccag ccagggcgcc gccagcgcgg
     3781 ccagcctggc cggatcctgg atgtgcgagc cgtcggcgac cacggtgcgc gtgggaatgt
     3841 tcgccgccag gatctcgccc gagcggtcgc agatgaggcc gcgccgggcc tggatgacct
     3901 ggcggatcga gttcttctgg gcggcgatcg ccgcgtattc ctcgtgctta aagacctgca
     3961 ggtccacgag cctcgcgcag aagaggctgt aaacggcgcc gacgccaatg atggctatgg
     4021 agatgcgttt tcgtgaagcg catctcacgg gctgaacctc gagttggccg acgccgtggc
     4081 ggtgcccagg atcaggctgt ccccgttaag aaccggctgg ccaaggcggg tgattgcgtg
     4141 atccttgatc ggaatcatgg cgatgaatcc ctcggcgagg cggcgctgca gcgaggcgcg
     4201 ggaagtcagg ttggtgatgt gcgccgcgag cacctgattc gccgcgcgca attcggcggt
     4261 ctgccgttcg gcatcgcgcg tctgctggcc gatggcgtgc tgctggtttt tgatgtgcac
     4321 gaaggccagt ccgctcattc cggcaaagaa ggcggccagc agccagaaga ccacgggttt
     4381 gatttcgact cggttggtgg tgggattgcg gcgaagattg gacatggcgg ttacagtttt
     4441 tgcgcgaccc gcagcttggc gctgcgggaa cgcgggtttt cggcgatttc ggcgggcgag
     4501 gcggaaagcg ggcggggcag cagcaattca aaaatccgca gcggattcgg gcgcggttcc
     4561 ggccactcgg gccggtccac ccattcgttg ctgtgctcgc ggaagtaat
//
LOCUS       DATDAY010000334         2122 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_1002804, whole genome shotgun sequence.
ACCESSION   DATDAY010000334 DATDAY010000000
VERSION     DATDAY010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2122)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2122)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2122
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1002804"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1008
                     /locus_tag="VIS74_06665"
     CDS             <1..1008
                     /locus_tag="VIS74_06665"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016813 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear
                     amidines [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/carbamoylase family amidase"
                     /protein_id="HEY5792962.1"
                     /translation="IDSVPNGGWLDGCLNVLAGVEILRRLQAEFQGRPPITLRLVDWA
                     DEEGARFGKSLFGSSACSGTLDLDEARRLRDRDGIALPDALRELGIDLETAKTCGKEL
                     ENAAAYLELHIEQGPVLLDLDLPLGAVLGTFGVERHAITFHGQAAHSGSTPMNKRRDA
                     FLAAARMAPEIYRIAGRHGGVCTIGSCTTKPGIVTSVVEECRLTLDQRHLDAPALARM
                     LAEAKAASGQFAGEAKTTVAWDRLWRIEPRPFHPDLIALCDAAIHETCGQSHRLPSGP
                     LHDAAEVARAGVPTVMMFVQSLHGISHNKIEDTREAHLELAVTAFDKLADKTISWITG
                     K"
     gene            1352..1855
                     /gene="nuoB"
                     /locus_tag="VIS74_06670"
     CDS             1352..1855
                     /gene="nuoB"
                     /locus_tag="VIS74_06670"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HEY5792963.1"
                     /translation="MAYDSKIEGNIIFTKLDAAINWMRVNSLWPMPMGLACCAIELMA
                     ASSARFDIARFGAEVMRFSPRQSDVMIVAGTVTYKMALAVKRVYDQMAEPKWVIAMGA
                     CASSGGMYRSYAVLQGVDEIIPVDVYVSGCPPRPEALLDGLIKLQQKIMREPAIHDQK
                     HPRLAHA"
BASE COUNT          395 a          700 c          631 g          396 t
ORIGIN      
        1 atcgactccg ttcccaacgg cggctggctg gacggctgcc tcaacgtcct cgccggcgtc
       61 gaaatccttc gccgcctcca ggccgaattc cagggccggc cgcccatcac cctccggctc
      121 gtggactggg ccgatgagga aggcgcgcgc tttggcaaaa gcctcttcgg ctcgtcggcc
      181 tgctccggca cgctcgatct ggacgaagcc cgccggctgc gggatcggga cggcatcgcg
      241 ctgcccgacg cgctgcgcga acttggcatc gatctcgaaa cggcgaagac gtgcggcaag
      301 gaattggaga acgccgccgc gtatctcgaa ctccacatcg agcaaggccc cgtcctgctg
      361 gatctggatc tcccgctcgg cgcggtgctg ggcacgttcg gcgtcgagcg tcacgcgatc
      421 accttccacg gccaggccgc ccactccggc agcacgccga tgaacaaacg gcgcgacgcc
      481 ttcctggccg ccgcccggat ggctccggaa atttaccgca tcgccggccg ccatggcggc
      541 gtttgcacca ttggctcctg cacgacgaaa cccggcatcg tgacgagcgt cgtcgaggaa
      601 tgccgcctta ccctcgacca gcgccacctg gacgcgccgg ctctcgcccg catgctcgcc
      661 gaggcgaagg cggccagcgg gcaatttgcc ggcgaggcca aaacgaccgt ggcgtgggat
      721 cgcctctggc ggattgagcc ccggcccttt catccggacc tgatcgcgtt gtgcgacgcc
      781 gccattcacg aaacctgcgg gcaaagccac cgcctgccct ccggcccgct gcacgatgcc
      841 gccgaagtcg cgcgggccgg ggttcccacc gtgatgatgt tcgtccagag cctgcacggc
      901 atcagccaca acaagatcga ggacacgcgc gaggcgcacc tggaactggc ggtcaccgcc
      961 ttcgacaagc tcgcggacaa gacgatctcg tggattaccg gaaaatagcg ccatccgacc
     1021 gggatcagcg atcaggaaat attcaccctg aagggcaggc agagacatga agatagattg
     1081 gaattttctt catgggcttc atgctcttca tggtgagcac tccgcccatt gcgcgtttca
     1141 aatcaggcaa acttctcgaa gtggcttggc ggatccccaa aacccatcac gcaagcactc
     1201 tcggtaattg agcccaagga ttggcatcat tccacggcgg caagggcctg ccgccctcca
     1261 tctccagggt cttgaatcgg cgggtgagga ttttcacctt gctccttccg ttcccttcct
     1321 tcaggctgtg cgcatggctg tccaagcccc gatggcgtac gattccaaga tcgaggggaa
     1381 tatcatattc accaaactcg atgcggctat caattggatg cgggtcaact cgctctggcc
     1441 aatgccgatg gggctcgcct gctgcgcgat cgaactcatg gccgccagct ccgcgcggtt
     1501 tgacatcgcc cggtttggcg ccgaggtcat gcgattttcg ccccgccagt ccgatgtcat
     1561 gatcgtcgcc ggcacggtca cctacaagat ggcgctcgcg gtcaagcggg tttacgacca
     1621 gatggccgaa cccaagtggg tcatcgccat gggcgcgtgc gcgtccagcg gtgggatgta
     1681 tcgcagctac gcggtgctgc agggcgtgga tgagatcatt cccgtggacg tgtatgtctc
     1741 cggctgcccg ccccgcccgg aagctctgct tgacggtttg atcaagttgc agcaaaaaat
     1801 catgcgcgag ccggcgatcc acgatcaaaa acacccgcgc ctcgcccacg cctgactttt
     1861 aacaaaatgc ggaattcgaa atttgaaatg cgaaatgccc cctgcgcgcc aagcgcggct
     1921 tggaattccg catttcccat ttcgcattcc gaattctcct catgaccgct ccagatgcct
     1981 gcgcccgcgt gcgcgaaaag tttgccgatg cggtgctcgc ggaaaccgaa tttcgcgggg
     2041 aatattcgct cacggtggag gcggacgcgg ttcatgccat cggcgaattt ctgcgggacg
     2101 ggctggggtt tgattacctc at
//
LOCUS       DATDAY010000335         2170 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_2408313, whole genome shotgun sequence.
ACCESSION   DATDAY010000335 DATDAY010000000
VERSION     DATDAY010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2170)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2170)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2170
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2408313"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1549)
                     /locus_tag="VIS74_06675"
     CDS             complement(<1..1549)
                     /locus_tag="VIS74_06675"
                     /inference="COORDINATES: protein motif:HMM:NF019344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY5792964.1"
                     /translation="MSEPAMPSPEEIQRRLNELLRANFGANPQAQSEASAPPDPPTPP
                     AREVVFEFQYLPADIKRHLDRFVIRQDEAKKVLSIAVCDHYNHAKLVARREKEGETQP
                     LEYAKQNVILLGPTGVGKTYLVKHVAELIGVPFVKADATKFSETGYVGGDVEDLVREL
                     VDKADGDTFLAEHGIIYIDEIDKIAGSGTPSGRDVSGRGVQTALLKLMEETEVPLRSQ
                     NDLQGQIQAMMEMQRRGKATRETLNTRHILFIVSGAFDRLSGIIQKRQKQAQIGFAAA
                     PAADVSANPFREVSTHDFVEFGMEPEFIGRVPVRVVCDPLSEADLFQILKTSEGSLLR
                     QYERAFRAYAVDVHFRDDALEIIAARAAAEKTGARGLLTVCERLFRDFKFDLPGSGVS
                     RFEVDAALVERPREKLDLLLRQSETQKAEILAQSARDYAAHFSQEFGLDLEFAPETVS
                     AIVARAQKEGISVIELCQRLFKDYQFGLKLLAKSSERPRFLIPPRAVEDPEGVLSEWV
                     VSSYRNNP"
     gene            complement(1546..>2170)
                     /locus_tag="VIS74_06680"
     CDS             complement(1546..>2170)
                     /locus_tag="VIS74_06680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792965.1"
                     /translation="IAADGRYAEARGIFSQLAQDVSVKRPDSDWLQLNIGLCQLLEND
                     SEAAEATFQELKKRELFSVEEALSPLASFFPEAARIMTLPGPVAPGTMKAYARDNYEA
                     FAFLLYGLKNWQFGQLEPAGEILRQFLACRPSGQWDWILEYQPLAQKFLHDFDVFEPL
                     NKDLKTATTPETKAALLERVREAKGQVQTGAAIEARLDEMEARLSRS"
BASE COUNT          384 a          699 c          665 g          422 t
ORIGIN      
        1 cggggttgtt tcgataggac gaaaccaccc attcactcaa tacgccctcg ggatcctcca
       61 ccgcgcgcgg cgggatgaga aaacgcggac gctccgagct tttggccagc agcttcaagc
      121 cgaactggta gtctttaaac aatctttggc aaagctcaat cacggaaata ccctcttttt
      181 gcgcccgcgc gacgatggcg ctcacggtct ccggcgcaaa ttccagatcc agtccgaatt
      241 cctgcgaaaa atgggcggcg taatcccgcg ccgactgcgc gaggatttcc gccttctggg
      301 tttcgctctg gcgaagcagg aggtcgagtt tctcccgggg gcgctccacc agggccgcat
      361 ccacctcgaa gcgcgaaacc ccggagccgg gcaggtcaaa tttgaagtcc cggaacagcc
      421 gctcacagac cgtcaataac ccgcgcgcgc cggtcttttc cgcggccgcc cgggcggcga
      481 tgatctccag cgcgtcgtcc cggaaatgca cgtccacggc gtaggcgcgg aaggcgcgct
      541 cgtattggcg cagcaggctg ccctcggagg ttttgagaat ctgaaacagg tcggcttcgc
      601 tcagcggatc gcacaccacg cgcaccggca cgcggccgat gaattccggc tccatgccga
      661 attccacaaa atcgtgcgtg gaaacctcgc ggaagggatt cgccgacacg tccgccgcgg
      721 gcgccgcggc aaagccgatc tgcgcctgct tctgccgctt ctggatgatt ccggaaaggc
      781 ggtcgaaggc gccgctgacg atgaaaagaa tgtggcgcgt attgagggtt tcccgcgtgg
      841 ccttgccccg gcgctgcatt tccatcatgg cctgaatctg cccctgcagg tcgttctggc
      901 tgcgcagcgg cacctcggtt tcctccatca atttcaacaa tgcggtctgc accccgcggc
      961 cgctcacgtc ccggccggag ggggtgccgc tgccggcgat cttgtcgatt tcatcaatgt
     1021 aaatgatgcc atgctcggcg aggaacgtat cgccatccgc cttgtccacg agctcgcgga
     1081 cgaggtcctc gacatccccg ccgacgtagc cggtctcgct gaacttggtg gcgtccgcct
     1141 tgacgaacgg cacgccgatc agttccgcca cgtgcttgac gaggtaggtt ttgcccacgc
     1201 cggtggggcc gaggaggatg acgttctgct tcgcgtattc cagcggctgg gtctcgccct
     1261 ccttctcgcg ccgggccacc agcttcgcgt ggttgtagtg atcgcacacc gcgatgctca
     1321 ggaccttctt cgcctcgtcc tgccggatca caaaccggtc gagatgccgt ttgatgtccg
     1381 ccggcaggta ttgaaattca aacaccacct cgcgcgccgg cggggtggga gggtcgggcg
     1441 gggcggacgc ctcgctctgc gcctgcggat tcgcgccgaa attcgcgcgc aacagttcgt
     1501 tcaaccggcg ctggatttcc tccggggatg gcattgcggg ctcgctcatg accgggaaag
     1561 ccgggcttcc atttcatcca ggcgggcttc gatcgccgcg cccgtctgca cctgcccctt
     1621 ggcctcgcgc acccgttcga gcagcgcggc tttggtttcc ggggtcgtgg cggttttcaa
     1681 atccttgttc aagggttcaa acacgtcgaa atcgtggagg aatttttgcg cgagcggctg
     1741 gtattccaaa atccagtccc attggccgga cggtcggcag gcgagaaatt gccggaggat
     1801 ttccccggcg ggttccagct gcccgaactg ccagttcttg aggccgtaga gcaaaaacgc
     1861 gaaggcttca taattgtccc tagcataagc cttcatcgtg ccgggcgcaa ccggaccggg
     1921 caaagtcatg atccgcgcgg cctcggggaa gaaactggcg agggggctga gcgcttcctc
     1981 cacggaaaac agttcccgct tcttcagctc ctgaaaggtc gcctcggccg cctcggaatc
     2041 gttctccagc agctggcaga gtccaatgtt caactgcagc cagtccgaat cgggccgctt
     2101 caccgaaacg tcctgggcga gctgggaaaa aatgccgcgg gcctcggcgt agcggccgtc
     2161 cgcggcgatc
//
LOCUS       DATDAY010000336         2390 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_1927587, whole genome shotgun sequence.
ACCESSION   DATDAY010000336 DATDAY010000000
VERSION     DATDAY010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2390)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2390)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2390
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1927587"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(266..784)
                     /locus_tag="VIS74_06685"
     CDS             complement(266..784)
                     /locus_tag="VIS74_06685"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HEY5792966.1"
                     /translation="MKRTWIFLGLATGAAALLAWLFWPAPRAQPLTGVDRILVEKGRR
                     QLSLLRGGEVLKTYRVVLGKSPIGHKTQEGDQKTPEGVYEIDGRNPQSAFHLSLRISY
                     PSAADRAQAEARGVSPGCDIMIHGLPNGAADPAAPRWREDWTAGCIAVSDAEIREIWA
                     AVPDGTAVEIRP"
     gene            845..1105
                     /locus_tag="VIS74_06690"
     CDS             845..1105
                     /locus_tag="VIS74_06690"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY5792967.1"
                     /translation="MIIRNETAGRFEARIGGQLAVAEYRIEDRRMIFTRTFVPPELRG
                     RGIAEKLVRAGFAAARAEGLAIVPECGYVARLLERHPEFQKP"
     gene            1102..1716
                     /locus_tag="VIS74_06695"
     CDS             1102..1716
                     /locus_tag="VIS74_06695"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY5792968.1"
                     /translation="MNRVIRPEILDHLDPRDPAAQRSRKDLRRIHFLMGNFRWMTRVI
                     PPARRIVEIGAGDGELCARLSRRFPAARVTGVDRIPGPPGVDWKQGDLFAVLPGCSGG
                     VLAGVMILHHFSGEALRLLGGLADGFERLCFCEPWRSSLSLAWGRVMNPFFSEVTRHD
                     LPASVDAGFRPGELAGLLGLDAARWRIAETVDWRGALRFQAWRR"
     gene            1722..>2390
                     /locus_tag="VIS74_06700"
     CDS             1722..>2390
                     /locus_tag="VIS74_06700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792969.1"
                     /translation="MTRGITIAGGGLAGLSLGIGLLRRGIPVELHEAGSYPRHRVCGE
                     FICGVSPATLESLGIHESLAGSRRLSSAAWFRRGERLLEAPLERPAWGISRYELDNRL
                     RRQFQKAGGDLRERSRLKNESREGLVWSAGRRPERGNWLGLKCHVRGLALDHDLEMHL
                     GRGGYAGLSRIEDGRVNVCGLFRKRAGLEGRGSELLLAYLRAGGLEILAGKIRGCIRD
                     EDSFL"
BASE COUNT          392 a          743 c          817 g          438 t
ORIGIN      
        1 ttcgttgatc ggcagcattt gcacaaatcc gaggccgaga tcggcggccc attttaccag
       61 ctcgcgcagc gcgcccacgt cgcccaatcc cagatcgtga tctccgcgaa tggaaaaaag
      121 aggcgcaagg acaccggcaa ttcgtttatc aggagtcaga tcgagcatgc gtctttctag
      181 cgaatgatcg gcaaggtgag aatctcaaac cgaagaaaat ccatccggcc tggaaaatga
      241 gcgggggtca tcgtcacgga gtgggtcagg ggcggatttc caccgcggtt ccgtccggca
      301 cggcggccca gatttcccgg atttccgcat cgctcaccgc gatgcagccg gccgtccaat
      361 cctcgcgcca gcgcggggcg gcgggatccg cggcgccatt gggcaaaccg tggatcatga
      421 tgtcgcaacc cggcgaaacg ccgcgggctt cggcctgcgc gcggtcggcg gcgctgggat
      481 aggaaatccg cagggacaaa tggaacgcgc tttgcggatt ccgtccgtca atttcgtaaa
      541 ctccctccgg cgttttctgg tcgccttcct gcgtcttgtg accgatgggg ctttttccca
      601 agacgacgcg gtaggttttc agcacctcgc cgccgcgcag cagcgaaagc tggcggcggc
      661 ctttctcgac caggatccga tccaccccgg tgagcggctg ggcgcgcggg gccggccaaa
      721 acagccaggc cagcagggcg gcggcgccgg tggcgaggcc gaggaagatc caggtgcgtt
      781 tcatgcggga ttgccaatcg agtgaatccg gaccatagca ggtaaccgag cctttcacca
      841 acccatgatc atccgaaacg aaacggccgg ccgctttgag gcgcggatcg gcgggcaatt
      901 ggcggtcgcg gaataccgca tcgaagatcg ccgcatgatt ttcacccgca cctttgtgcc
      961 gccggaactg cgcggccgcg gaattgccga aaagctggtg cgggcgggat tcgcggcggc
     1021 ccgggcggag gggctcgcca tcgtgcccga atgcggttac gtggcgcgcc tgctggaacg
     1081 gcatccggaa tttcaaaagc catgaatcgc gtcatccgtc cggaaattct cgatcacctc
     1141 gatccccggg atcccgccgc gcagcgcagc cggaaggatc ttcgccgcat tcattttctc
     1201 atgggaaatt ttcgctggat gacgcgggtt attcccccag cgcggcgcat tgtcgaaatc
     1261 ggggccggcg acggcgagct ttgcgccagg ctgtcccggc ggtttccggc ggccagggtg
     1321 acgggcgtgg atcgcatccc cggcccgccg ggggtggatt ggaaacaggg cgacctgttc
     1381 gccgtgcttc ccggctgctc cggcggcgtg ctggcgggcg tcatgatcct gcatcatttt
     1441 tccggcgagg cgctgcggct tctcggcggg ctggcggatg gatttgagcg cctttgtttt
     1501 tgcgagccct ggcgctcgtc cctgagcctg gcctggggcc gggtgatgaa tccatttttt
     1561 agcgaggtga cccgccacga tctgccggcc agcgtggacg cgggttttcg cccgggagaa
     1621 ttggccggac tgctgggcct ggatgcggcc cgctggcgga tcgcggagac ggtggactgg
     1681 cgcggcgccc tccgcttcca ggcctggcgg cgatgactgg aatgacgcgt ggcatcacca
     1741 tcgccggcgg cggattggcc ggactttcgc tgggcatcgg cctgctccgg cgcggcattc
     1801 cggtcgaact gcacgaggcg ggaagttatc cccggcaccg cgtttgcggt gaatttatct
     1861 gcggggtcag cccggcaacc ctggaaagcc tgggaattca cgaatcgctg gccggttccc
     1921 ggcggctttc ctccgcggct tggtttcggc ggggcgagcg gctgctcgaa gccccgctcg
     1981 aacggccggc ctggggcatt tcccgttacg aactcgataa ccggctgcgc cggcaattcc
     2041 agaaagccgg cggcgatctg cgcgaacgct cccgcctcaa aaacgaatcc cgcgaaggcc
     2101 tcgtctggag cgccgggcgc cggcccgaac gcgggaactg gctggggctc aaatgccatg
     2161 tgcgcggact cgcgttggat cacgacctcg aaatgcacct cggccggggc ggttacgccg
     2221 ggctttcccg catcgaggac ggccgggtga atgtctgcgg tttgtttcgc aagcgtgcgg
     2281 gcctggaagg ccggggctcg gagctgctgc tcgcgtatct gcgagcgggc gggctcgaaa
     2341 tcctcgcggg aaaaatccgc gggtgcattc gcgacgagga ttcctttctg
//
LOCUS       DATDAY010000337         2542 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_2190501, whole genome shotgun sequence.
ACCESSION   DATDAY010000337 DATDAY010000000
VERSION     DATDAY010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2542
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2190501"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            279..1025
                     /gene="gpmA"
                     /locus_tag="VIS74_06705"
     CDS             279..1025
                     /gene="gpmA"
                     /locus_tag="VIS74_06705"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: protein motif:HMM:NF010713.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HEY5792970.1"
                     /translation="MIKLVLIRHGESAWNKENRFTGWTDVDLTDKGREEAASGGKLMK
                     AEGFTFDVAYVSVLKRALRTLWTALDEMDLLWIPIEKSWRLNERHYGALQGLNKSETA
                     EKFGEDQVKIWRRSYDIPPPPLEKSDERWPGNDPRYAGIPEAELPLTECLKDTVARFL
                     PLWQETIAPVVKSGKRVIIAAHGNSLRALVKYLDNLSEEEVLGLNIPTGVPLVYELDD
                     ELKPIRHYYLGDQAAIEAAMAAVANQGKAK"
     gene            complement(1111..1980)
                     /locus_tag="VIS74_06710"
     CDS             complement(1111..1980)
                     /locus_tag="VIS74_06710"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEY5792971.1"
                     /translation="MAEILIAGCGFLGEAAAALFLEAGWKVHGLTASEKSADALGERG
                     VIAHAGDLTQLPSVAALRQKIGRMEAVIHCASSGRRGEPAYRAVYESGMRHLGAMFPE
                     ARLLFVSSTSVYGQVNGEWVHEESPAEPSRETSRILLAAEQVALRTDGWVARVAGIYG
                     PGRSVYLRRFLSGEAVLENGGSRWVNQIHRDDAARGLFQLIQTRAPAGVYNVSDDAPC
                     SLKEIYSWLGGYFGKPLPPEGEGDPNRKRALTNKRVRNAKLRSTGWQPRYPSYREAIA
                     ELERWQNETGFPD"
     gene            complement(1992..2189)
                     /gene="rpsU"
                     /locus_tag="VIS74_06715"
     CDS             complement(1992..2189)
                     /gene="rpsU"
                     /locus_tag="VIS74_06715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEY5792972.1"
                     /translation="MPEVYVKKGEPIDRALKRLKSKLDNEGILDEVRRLRAFETPTQK
                     SRRKAKANAKRAKLKQRFHFR"
     gene            complement(2299..>2542)
                     /locus_tag="VIS74_06720"
     CDS             complement(2299..>2542)
                     /locus_tag="VIS74_06720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792973.1"
                     /translation="VRYARDFDVDGVLCGHIHTPAIRAIGGVEYFNTGDWVESGTAIV
                     EHFDGRMELIRHPLSGFAGESPELLLPLMRREKAGA"
BASE COUNT          524 a          814 c          718 g          486 t
ORIGIN      
        1 aaatccctgc tccacttcct gcaagaacag gatgtccgca ttcaggccgc gcaggaaagc
       61 gatcgtctgc tccaagtcga tggcttcacg gtccggatcg tcctcccgcc acggctggcc
      121 gttttgcatg ttgaatgtcg cgacccggat ttgcatcgaa gccaaagatt caactgggct
      181 ttgcgccgca agtccagccc gggcgcaggc aattccgggc gaacgaaaat ttcccgagat
      241 tgaccaaagc gcggcatgtt ctatgctccc gctttcgtat gatcaaactc gttttaattc
      301 gtcacggcga gagcgcctgg aacaaggaaa accgctttac cggctggacc gacgtggatc
      361 tgaccgacaa gggccgcgag gaagccgcca gcggcggaaa actcatgaaa gccgaaggat
      421 tcacgttcga cgtcgcttat gtgtccgtgc tcaagcgtgc gctgcgcacg ctctggaccg
      481 cgctggacga gatggacctg ctgtggattc cgattgaaaa atcctggcgg ttgaacgagc
      541 ggcattacgg cgccctgcag ggcttgaaca aatccgagac cgcggaaaaa ttcggcgagg
      601 accaggtcaa aatctggcgc cgcagttacg acattccgcc gccgccgctt gaaaaatccg
      661 acgaacgctg gccgggcaac gacccgcgct acgccggcat cccggaagcc gaacttccgc
      721 tcacggaatg cctcaaggac accgtggcgc gcttccttcc gctctggcag gaaacgatcg
      781 cgcccgtcgt gaagtctggc aagcgcgtca tcatcgccgc ccacggcaac agcctgcgcg
      841 cgctggtcaa atacctcgac aatctttccg aagaggaagt cctcgggctg aacattccga
      901 ccggcgtgcc gctggtctat gagctcgacg acgaactcaa gccgatccgc cattattacc
      961 tcggcgatca ggctgccatc gaagccgcca tggccgcggt ggccaatcag ggcaaggcga
     1021 agtaacgccg cgttccggaa cgaaacggga ttgactggat ttccaggatt aacgggattt
     1081 tttggggacg gctggcatcc gggaatcccg ttaatccgga aatcccgttt cgttttgcca
     1141 gcgctccagc tccgcgatcg cctcgcggta gcttggatac ctgggctgcc agccggttga
     1201 tcgcagcttg gcgttgcgga cgcgtttgtt cgtgagcgcg cgcttgcggt tgggatcacc
     1261 ttccccttcg ggcggcagcg gtttgccgaa atacccgccg agccagctgt aaatttcctt
     1321 cagcgaacaa ggcgcatcgt cgctgacatt ataaacgccg gccggggcgc gggtttggat
     1381 caactggaag agtccccgcg cggcatcgtc gcggtggatt tggttcaccc aacggctccc
     1441 gccattttcc agcacggctt cgccggacaa aaatcggcgg aggtaaacgg aacggcccgg
     1501 gccgtaaatc cccgccacgc gcgccaccca tccatcggtg cgcagcgcca cctgctcggc
     1561 ggcgagcagg atccggctcg tctcgcggct gggttcggcg gggctttcct catgaaccca
     1621 ttccccattc acctggccgt aaaccgaagt gctgctgaca aacagcaggc gcgcctcggg
     1681 aaacatcgcg ccgaggtgcc gcatgccgct ttcataaacc gcgcggtagg ccggctcccc
     1741 ccggcgcccg gaactggcgc aatgaatgac cgcctccatg cgcccgatct tctggcgcag
     1801 cgcggccacg gagggcagct gggtgagatc gcccgcatgc gcgatcacgc cgcgctcgcc
     1861 gagggcgtcc gcggattttt cggaagccgt caacccatga actttccagc cggcctccaa
     1921 aaacaaagcg gctgcggctt cccccaggaa accgcagccg gcaattaaaa tttcagccaa
     1981 gagcggaaaa attaacgaaa atggaaacgc tgcttgagtt tcgcgcgctt cgcattcgct
     2041 ttggctttac ggcgtgattt ttgcgtgggc gtttcgaacg cgcgcaaacg acgcacttcg
     2101 tcaagaatgc cctcattgtc gagcttcgac ttcagacgct tcaacgcccg atcaatcggc
     2161 tcgcctttct tcacataaac ttctggcata ttgtcaccat cctttcttca aaaattcgag
     2221 agtttttgac cttaccgcaa agcgaacgcc cctgtcaaac ccggcgtttc cccattgcgc
     2281 attcgaattt ctgaagaatc aggcgccggc cttttcccgc cgcatgaggg gcagcaaaag
     2341 ctccgggctt tcccccgcaa agccggaaag cgggtgccgg atcaattcca tccggccatc
     2401 gaaatgctcg acaatggccg tgccgctttc cacccagtcg ccggtattaa aatattccac
     2461 gccgccgatg gcccgaatcg ccggcgtgtg gatgtgcccg cacagcacgc catccacgtc
     2521 aaaatcccgc gcgtaacgca cg
//
LOCUS       DATDAY010000338         2447 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_1702609, whole genome shotgun sequence.
ACCESSION   DATDAY010000338 DATDAY010000000
VERSION     DATDAY010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2447)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2447)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2447
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1702609"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(67..139)
                     /locus_tag="VIS74_06725"
     tRNA            complement(67..139)
                     /locus_tag="VIS74_06725"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(104..106),aa:Phe,seq:gaa)
     gene            complement(217..735)
                     /locus_tag="VIS74_06730"
     CDS             complement(217..735)
                     /locus_tag="VIS74_06730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792974.1"
                     /translation="MRPLLCGLLSALLLSAAHAQTPKDAKNRFVFDPGATTPQTPAPP
                     PAPALTPPQTLESFFNLLKQEKISPAYDGLVAGTILGERKEDVEALKNKTQQAIDSYG
                     SLSGYEIIDTLTAGKNLLRYTCISLNEDLPLRWRFYFYNGPKGWRLVDLRIDDGLVEL
                     FESIKQRNPPAP"
     gene            806..1309
                     /locus_tag="VIS74_06735"
     CDS             806..1309
                     /locus_tag="VIS74_06735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792975.1"
                     /translation="MKLSPRLVLAALALPLFGCATTPPKETRPAYVYQLPKMGVSPGT
                     YSRIYNHRVLTFADILDLVTHNVPDDKIVAYLRGTHAPYQFTVKQINQLAAAGAGSTL
                     VNYLGQGAGAYLIDASDSAAQQQLRQQAMDEKYWRHAYFTDPYYYGAAPFNYGWPGEW
                     GPMMPLY"
     gene            complement(1406..2305)
                     /locus_tag="VIS74_06740"
     CDS             complement(1406..2305)
                     /locus_tag="VIS74_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HEY5792976.1"
                     /translation="MQERIEEFIQFLAVERGLSANYQLSIRHSLETFHAWARSQSRPG
                     AADIRAEHLSEYLAHRKRLGLAAGSIKAEAVALRIFFRHLCARKHLAADPSEFLGIPR
                     IERTLPHTLSLEEVEKLLNAIPAETAIGRRDRAIVELLYGCGIRVSELCGIRLEDYHA
                     TERILRVTGKGNKTRLLPIGKPAVAAVETYLERGRPELVGKRTGAQVFISIRGRALTP
                     QRIWQLLREYAPAAGLEEKIYPHLLRHSFATHLLGNGADLRVIQELLGHADIATTEIY
                     THVDTRRLQETHRRFHPRSRIKP"
BASE COUNT          435 a          733 c          798 g          481 t
ORIGIN      
        1 tataggcgga ctcgccgatc gtgatcgtcg tccacatcac ataagttact tatgggcagc
       61 taggtatggc tggggaggga atcgaacccc cgacacgagg attttcagtc ctctgctctg
      121 ccaactgagc tacccagcca gtgaatctcc ttgcgggaag agtgggatac tttcctcgct
      181 tcccgccttt cgggcaagga aaatgattcg ccgaatttac ggggcgggcg ggttgcgttg
      241 tttgatggat tcaaacagct cgaccaggcc gtcgtcgatc cgcaggtcca ccagccgcca
      301 gcctttgggg ccgttgtaga aataaaaccg ccagcgcagc ggcaaatcct cgttcagcga
      361 gatgcaggtg taacgcagca gatttttccc ggcggtcagc gtatcgatga tttcgtagcc
      421 cgagagggag ccatacgaat caatcgcctg ctgggtcttg tttttgagcg cctccacgtc
      481 ttccttgcgc tcgcccagga tggttccggc gacgagccca tcgtaggcgg gggagatctt
      541 ttcctgcttc agcagattga agaatgactc gagcgtctgc ggcggggtga gcgcgggagc
      601 gggcggcggg gcgggcgttt gcggagtagt tgcgccgggg tcgaacacaa accggttctt
      661 ggcatccttg ggggtctggg cgtgagccgc ggaaagcaga agcgcggaga ggagtccgca
      721 aagaagtggg cgcatccggc caatatgccg ccgccggcgg aaattgcaat tgccccgcgc
      781 cggctgccgc cgtagaaaag cgggcatgaa gctgtctccc cgcctcgttc ttgccgccct
      841 ggctctgccc cttttcggat gcgccacgac ccctccgaag gaaacgcgtc cggcgtatgt
      901 ctatcaactc cccaaaatgg gcgtaagccc gggcacctat tcccgcatct ataatcaccg
      961 cgtgctgacg ttcgcggaca ttctcgatct ggtgacgcac aatgtgccgg acgacaagat
     1021 cgtcgcctac ctccgcggta cccatgcccc gtatcaattc acggtgaagc agatcaacca
     1081 gctggccgcc gccggcgcgg gaagcacgct tgtgaattac ctcggccagg gcgcgggcgc
     1141 gtatctcatt gacgcctccg actccgccgc ccagcagcag ctccgccagc aggccatgga
     1201 cgagaaatac tggcggcacg cctactttac cgatccctat tactacggag cggcgccgtt
     1261 taactacggc tggccgggcg aatggggccc gatgatgccg ctttattaag cgggcgcatt
     1321 tttgggttaa ttaattggac gcgctcctcc cggcggcgtt ggcaacgccg gtcacggtga
     1381 caaaaataaa tggaaaacgc ccggcctacg gcttgatccg gctgcgcgga tgaaagcgcc
     1441 ggtgggtttc ctgcaggcgc cgcgtgtcca catgggtgta gatttccgtg gtggcgatgt
     1501 cggcatggcc gagcagctcc tggatgacgc gcaggtcggc gccattgccg agcaagtgcg
     1561 tggcgaagga atgccgcagc aggtgcggat aaattttttc ctccagcccg gcggcgggcg
     1621 cgtattcccg caggagctgc cagatgcgct ggggggtgag cgcccggccg cggatggaga
     1681 tgaacacctg ggcgccggtg cgtttgccca ccagctccgg ccggccgcgc tcgaggtagg
     1741 tttccacggc ggccacggcg ggtttgccaa tgggaagcag ccgggtttta ttgcccttgc
     1801 ccgtcacccg gaggatgcgt tcggtggcgt ggtaatcctc caagcggatg ccgcaaagct
     1861 cgctgacccg gatgccgcag ccgtagagca gttccacgat cgcccggtcc cgccggccga
     1921 tggccgtctc ggcgggaatg gcgttcagca atttctccac ttcctccagc gaaagcgtgt
     1981 gcggcagggt gcgttcgatg cgtggaatgc cgaggaattc cgagggatcg gcggccaggt
     2041 gttttcgggc gcaaaggtgg cggaaaaaaa tccgcagcgc cacggcttcg gccttgatcg
     2101 atcccgccgc gagcccgagg cgcttgcggt gcgcgaggta ttcgctcaga tgctcggcgc
     2161 ggatgtccgc cgcgcccggc cggctttggc tccgcgccca ggcgtggaag gtttccagcg
     2221 agtgccggat ggaaagttga tagttggcgg acaggccgcg ttcgaccgcg agaaattgaa
     2281 tgaactcttc gatgcgctcc tgcattacgg cagtttgagc ccggtcagcc gttcgtaggc
     2341 ctcgacgtat ttttccgagg tgcgccgcac gacttcggcg ggcagcttcg ggccgggcgg
     2401 ctttttgtcc caatccagcc cctcgaggta gtcccgcacg tattgct
//
LOCUS       DATDAY010000339         3870 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_2258185, whole genome shotgun sequence.
ACCESSION   DATDAY010000339 DATDAY010000000
VERSION     DATDAY010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 3870)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3870)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3870
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2258185"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(64..1539)
                     /locus_tag="VIS74_06745"
     CDS             complement(64..1539)
                     /locus_tag="VIS74_06745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792977.1"
                     /translation="MPSDPKKIPELVTRRQKANLPKHETALNFFSNSLFAWTLFILLL
                     IGIAIVSWFGSFYIFGHPEEPLNYQILSKLKKIEEPKRFELTEAPRGEFLTANELLAR
                     YGKLTRRELAKVNGELLRNYIRNFNQTKAPLAYIVGNFNILDSYELKSANFFPTGVVA
                     LAQSTENSRVLLEHVFTADPKTIPTLHRTLLTGLDIDLKRRQDISAVIHVEKLKDGRL
                     KLTAIPLLYGSYASTEGPGSFSLEPPAVLNPEGGLPILGQERVDEALQKYAQYRRRAG
                     LSESEPGVPRATAANQLTRVERPPDVAPTPSPAPPAVAVVTPEPRVLPAMPVATPVPT
                     ATPLLQPFLAASPSPTLTPSPTPTPATPAPAPTRTPEAAIASAAGRNWQVYGPGQMPR
                     GRLLNLPDVPELANRGLGGERLYLQGNFLVTASGSGRAVLRSSSRSGKVRIIVDYPAG
                     STPPVTDSNFSRDSRRPFLITDVKPAEDGFVNVFVREVTRP"
     gene            complement(1542..1943)
                     /locus_tag="VIS74_06750"
     CDS             complement(1542..1943)
                     /locus_tag="VIS74_06750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HEY5792978.1"
                     /translation="MAEELQFLFVVEFDSGESLHNDNYMIHITEAAAKQLHALAEEKS
                     LPGTGGLRLAVEKGGCAGLQYAMTLATPETGDTIEEAFGARVLVDPESLAYLDGSTLD
                     YEDGLSGAGFRIHNPKATRSCGCGTSFEPAA"
     gene            1942..3708
                     /gene="mutL"
                     /locus_tag="VIS74_06755"
     CDS             1942..3708
                     /gene="mutL"
                     /locus_tag="VIS74_06755"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HEY5792979.1"
                     /translation="MGRIRLLPEQVASQVAAGEVVERPASVVKELVENSLDAGARRIE
                     IEFARGGAGLIRIADDGSGMDRDDALMSLERHATSKIRAAADLATVGTFGFRGEALPS
                     IASVSKFRMVTRPREAEAGTEIVAAGGRVLEVRDAGEAFGTSIEIRSLFYNVPARLKF
                     LRGEQTEAGHLLHQITLAALARPEAGFGVIRDGRLWKQLPAAASLGGRVRDLFGGGAA
                     SQFQEVGAFEWQGIRLRGFFAKPGAGRLDKAQQFVFINGRPVQNPGISQGFREACAGV
                     LERGMNPAAIVFIELEPAAFDCNVHPAKREVRFREPLKIREAAYEFARHALSLGRAKP
                     ATVSAPVELSAPVSLPKAVEFRPRVTAEQMPLTSPPSLPLAGQARMAQPAPPVAAAVP
                     EKRLPDFRLIGPLGKNYLLLESAEGLVTLELAAARERIDYETLCRQMAAGGCPSQGLL
                     PPVVVNLDPRDFSFAVEHLESLRAAGLQAEAFGPAAVKIDALPAGLADARPGELLLRL
                     LDEIREGIRSSARRFVRDWVALALAKAAGERMIGNFPAAAAQDLVQQLMACELPYASP
                     TGRPTLLQTSWSELARKFGKSP"
BASE COUNT          649 a         1112 c         1425 g          684 t
ORIGIN      
        1 aggtattcgg agaacgccag ggattcgcgg ccgccgagtc gttccgcgaa ggcggagacg
       61 aactcagggg cgggtgactt cgcggacgaa gacattcaca aaaccgtcct ccgccggttt
      121 gacgtcggtg atgaggaagg ggcggcggga gtccctcgag aagttggaat ccgtcaccgg
      181 cggggtggag ccggcggggt agtccacgat gatgcggacc ttgccggagc ggctcgaact
      241 gcgcaggacc gcgcggccgc tgccggaggc ggtcacgaga aaattcccct gcaggtaaag
      301 gcgttcgccg cccaggccgc ggttggccag ttccggcacg tccggcaggt tcaagaggcg
      361 gccgcgcggc atctggcccg ggccgtaaac ctgccaattg cgtccggccg cgctggcgat
      421 ggcggcttcc ggcgtccggg tgggagcggg agctggagtg gcgggggtgg gcgtgggaga
      481 tggggtcagc gttggcgagg ggctggccgc caggaagggt tgcaacagcg gcgtggccgt
      541 cggaaccggg gtggccacgg gcatggcggg aagcacgcgg ggttccgggg taaccacggc
      601 aaccgccggc ggagccgggg agggggtggg cgcaacatcc ggcggacgct cgacgcgggt
      661 gagttgattg gcggcggtgg cgcgcggcac gcccggttcg gattcactta aaccggcccg
      721 gcggcggtat tgcgcatact tttgcaaggc ttcgtccacc cgctcctgcc cgaggatcgg
      781 aagtccgcct tcggggttca ggacggcggg gggttccaat gagaagctgc cggggccttc
      841 cgtcgaggcg taactgccgt aaagcagcgg aatggcggtg agtttcagcc ggccatcctt
      901 caatttttcg acatggatga cggcggaaat gtcctgccgg cgcttgagat cgatatccag
      961 gccggtcaac agcgtgcgat gcagcgtggg gatggttttg ggatcggcgg taaacacatg
     1021 ctccagcagc acccgggaat tttcggtgga ttgggccaag gccaccacgc cggtcgggaa
     1081 aaaattcgcg gatttgagtt cgtaggagtc caggatgttg aaatttccga cgatgtaggc
     1141 gaggggggcc ttggtctgat tgaagttgcg gatgtagttc cgaagcagtt cgccattgac
     1201 cttggccaat tcgcggcggg tgagcttgcc gtagcgcgcg agcagttcgt tggccgtcaa
     1261 aaattccccg cgcggggctt cggtgagctc aaagcgcttg ggttcctcga ttttcttcag
     1321 cttggaaaga atctggtagt tgagcggttc ctcggggtgc ccaaaaatgt agaagctgcc
     1381 aaaccaggaa acgatggcga tgccgatcag cagcaggata aagagggtcc acgcgaacag
     1441 cgagttgctg aagaagttga gcgccgtctc gtgcttgggg aggttggctt tctgccggcg
     1501 ggtgacgagc tcgggaatct ttttgggatc ggaaggcatg gtcaggcagc cggctcgaag
     1561 gaggttccac agccacagct gcgggtggct ttgggattat ggatgcgaaa cccggcgccg
     1621 ctgaggccgt cttcgtaatc gagggtggag ccatccagat aagccaggct ttcgggatcc
     1681 acgagaaccc gggcgccgaa cgcttcctca atggtatcgc ccgtctcggg ggtggccagc
     1741 gtcatcgcat attgcaagcc ggcgcagccg cctttttcca ccgccaagcg caacccgccc
     1801 gtgccgggga gcgatttttc ttcggccagc gcgtgcaatt gttttgccgc tgcttcggtg
     1861 atgtgaatca tgtaattatc attatggagc gactcgccgg agtcaaattc gacgacaaac
     1921 aaaaattgca actcttccgc catgggacgc atccgcctgc tgcctgaaca ggtcgccagc
     1981 caggtggcgg ccggggaggt ggtggagcgc ccggcttcgg tggtcaagga actcgtcgaa
     2041 aacagcctcg atgccggggc gcggcggatc gagatcgagt tcgcccgggg gggcgcgggc
     2101 ctcatccgca ttgccgacga cggcagcggc atggaccgtg acgatgcgct catgagcctc
     2161 gaacgccacg cgacgagcaa gatccgcgcc gccgcggatt tggcaaccgt ggggaccttc
     2221 ggtttccggg gggaggccct gccgagcatc gcgagcgtgt cgaaattccg aatggtgacg
     2281 cgcccgcgcg aggccgaggc ggggacggaa atcgtcgccg ccggcggccg ggtgctcgaa
     2341 gtgcgggacg cgggcgaagc gttcggcacg tccattgaaa tccgctcgct gttttacaat
     2401 gtccccgcgc ggctcaaatt cctgcgcggc gaacagacgg aggccggcca cctgctgcat
     2461 cagatcacgc tggcggcgct ggcccggccg gaggcgggtt tcggggtgat tcgcgacggc
     2521 cggctgtgga agcaattgcc cgcggcggca tcgctcggcg ggcgcgtgcg ggatttgttc
     2581 ggcggcggcg ccgccagcca gtttcaagag gtcggcgcgt tcgaatggca gggaatccgc
     2641 ctgcggggat tcttcgccaa gccgggggcg gggcggctcg acaaggcgca gcagtttgtg
     2701 ttcatcaacg gccggccggt ccagaatccg gggatcagcc agggcttccg ggaggcttgc
     2761 gccggagtgc tggaacgggg aatgaatccg gcggcgattg tgttcatcga actggagccg
     2821 gcggcgttcg actgcaacgt gcatccggcc aagcgcgagg tgcggttccg cgagccgctg
     2881 aaaatccgcg aggccgccta tgaatttgcc cggcacgcgc tgtcgctcgg ccgggcgaaa
     2941 ccggcaacgg tttcggcgcc ggtggagcta tccgcgccgg tttccctccc gaaggcggtc
     3001 gagttccgcc cccgcgtcac cgcggagcaa atgccgctga ccagcccacc gtccctgccg
     3061 ctggcgggtc aagcgcggat ggctcaaccc gcaccgccgg tggccgccgc cgttccggaa
     3121 aaacgcctgc ccgacttccg gttgattggg ccgctgggga aaaactatct cctgctcgaa
     3181 agcgccgaag ggctggtcac gctggagctg gcggcggcgc gggagcggat tgactacgaa
     3241 acgctctgcc ggcagatggc cgccggcggc tgccccagcc agggattgct gccgccggtg
     3301 gtggtcaatc tcgacccgcg cgatttttcc ttcgcggtgg agcatctgga aagcctgcgc
     3361 gccgccggtt tgcaggcgga ggcgttcggc ccggcggcgg tgaagatcga cgcgctgccg
     3421 gcgggcctgg cggatgcccg gcccggcgag ctgttgctcc ggctgctgga cgaaattcgt
     3481 gagggtattc gctcctccgc gcgccgcttc gtgcgcgatt gggtggcgct ggcgctggcc
     3541 aaggcggcgg gtgagcggat gatcgggaac ttcccggccg cggcggcgca ggatttggtg
     3601 cagcagttga tggcgtgcga attgccctac gcttcaccga ccggacggcc gacgctgctg
     3661 caaaccagct ggtcggaact ggcgcggaag tttgggaaga gcccttaaag cggcatcggc
     3721 atcctgccga ggatcgttgt ccacgcatga gcaggaagct catgccacgt gatttcattg
     3781 gctggaggtt gcctcgaact tttgcgagac gttcgcgctt ttggggacgc cggtgcggta
     3841 ttcggaggcg agcttgggcg ggatttgctg
//
LOCUS       DATDAY010000340         3841 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_121085, whole genome shotgun sequence.
ACCESSION   DATDAY010000340 DATDAY010000000
VERSION     DATDAY010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 3841)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3841)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3841
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_121085"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..642
                     /locus_tag="VIS74_06760"
     CDS             <1..642
                     /locus_tag="VIS74_06760"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEY5792980.1"
                     /translation="RLAGEIPGAWWANQFDNVANRLAHYHTTGPEIWRQTEGKITGFS
                     ASVGTGGTLAGTALALKERKDSIRVICPDPYGAAMWSWFKLGHTQVNDGDSFAEGIGQ
                     GRVTKNLENIPVDDAIRVPDALALRLLYHLLRHEGLFLSLSSAINVAGALRLALQQGP
                     GQTIVTILCDSGSRYLSKLYSPVWLNSHGFQHLLAPESVSADALVQSISQLPE"
     gene            729..1850
                     /locus_tag="VIS74_06765"
     CDS             729..1850
                     /locus_tag="VIS74_06765"
                     /inference="COORDINATES: protein motif:HMM:NF014691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HEY5792981.1"
                     /translation="MSPIKWNWEREDWPHFRYKAAELEKLEANFLLQAGVVSGAIRHI
                     NEEEKSLLTVELISNEALKTSEIEGEVLNRESLQSTIRRHFGLAADNRRISPAEQGLA
                     EMMSDLFYNFAAPLTHEILFGWHAMLMSGRRNLQDSGRYRTGRDPMQIVSGPLHEPKV
                     HFEAPPSQALAKEMEQFVGWFNRSAPDGNRPLPALTRAGIAHLYFVSIHPFEDGNGRI
                     GRAISEKSLSQSLNQPTLIALSHVINSRKKAYYNSLEQSNRHNEITGWLVDFARTILD
                     AQSYTQRRIDRLIEKAKFFDRFRGQFNERQEKAISRMFRGEPEGFPGGLSAEKYIRIT
                     GAARATATRDLQDLAAKGALTRTGTLKGTRYHLATLPTG"
     gene            complement(2001..2921)
                     /locus_tag="VIS74_06770"
     CDS             complement(2001..2921)
                     /locus_tag="VIS74_06770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY5792982.1"
                     /translation="MTALEDLSLLRFFVRIVESGSISAAARSLKMPQPTLSRHLRRLE
                     ENCGASLLRRDTHTMNLTEAGSRLYKDAGAILELVESAAQRVREDQTTIRGHLRLFAT
                     VDFGQSAVTRLITRFLLANPGVTAELKYSNRLTRMIDEGFDAGVVVGKISDDRVVARP
                     AGGVVRYLVAAPALAKKQGVATAPEDLEAWPWVSLGGGLFGDARKIVLSAPRKAGKEL
                     NIEPLLISEGVTSMREAVRGGAGVAVLPDWLVREDLIAGRLVRILPQWNAPDLPVHVI
                     HPSDRTLPARVRACVDFAVRYMTTEMHSAT"
     gene            3034..3780
                     /locus_tag="VIS74_06775"
     CDS             3034..3780
                     /locus_tag="VIS74_06775"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY5792983.1"
                     /translation="MNTSNPKVALITGSSRGIGAAIAKRLAQDGMRVVINYAGRAADA
                     EKVVQEITKAGGRALAIQADVSNPEEAARLFEQATAEFGGVDILVNNAGVMQPGLVPV
                     AETSDELFDRLIAINLKGTFNTLRLAAKQLRAGGRIVNFSTSVVGLALPGYAVYASTK
                     AAVETLTNIFAKEMRGRQITVNSVAPGPTATDLFLNGKTPEQIEHLAKMPPLERLGRP
                     EDIANVVSLLVGADGGWINGQTLRANGGIV"
BASE COUNT          791 a         1275 c         1091 g          684 t
ORIGIN      
        1 cgcctggccg gcgaaattcc cggcgcgtgg tgggccaatc aattcgacaa tgtcgccaac
       61 cgcctggcgc actatcacac caccggcccg gaaatctggc ggcaaacgga gggaaaaatc
      121 accggcttct ccgcctccgt gggaaccggc ggcacgctcg ccggcaccgc gctggcgctc
      181 aaggaacgca aggattccat ccgcgtcatc tgcccggacc cctacggcgc cgccatgtgg
      241 tcatggttca agctgggcca cacgcaggtc aacgacggcg actcgttcgc ggaaggcatc
      301 ggccaggggc gcgtgaccaa gaatctcgaa aatatcccgg tggatgacgc cattcgcgtt
      361 cccgatgccc tcgcgctgcg cctgctctat cacctcctgc gccatgaggg attgttcctc
      421 agcctgtcct ccgccatcaa cgtcgccggc gccctccggc tcgcactcca gcaaggcccc
      481 gggcagacga tcgtcaccat tctgtgcgac agcggctcgc gttatctttc caagctctac
      541 agccccgtct ggctgaattc ccatggattc cagcacctcc tcgcgccgga atccgtctcc
      601 gccgacgcgc tggtgcagtc aatctcccaa ttgccggaat gaaccaaacg ggctgccggg
      661 cgaaaagcgt tcgctttata gctcatattt cagcatatat tgagttataa ataggaattt
      721 tatagctcat gtcaccgata aaatggaatt gggaacgcga ggactggccg cattttcgat
      781 acaaggccgc cgagttggaa aaactggagg caaacttcct gcttcaggcc ggcgtcgtct
      841 cgggcgcgat ccggcatatc aatgaggagg aaaaaagcct cctgaccgtc gagctcatca
      901 gcaacgaagc actgaagact tccgagatcg agggggaggt tctgaaccgc gagagccttc
      961 aatccacgat tcggcgccat tttggcctgg cggccgacaa ccggcgaatt tctccggcag
     1021 aacaggggct cgccgaaatg atgtcggatt tattctacaa tttcgcggct ccgctgacgc
     1081 atgaaatcct gttcgggtgg cacgcgatgt tgatgagcgg ccgacgcaac ctgcaggatt
     1141 ccggtcgata tcgaaccggc cgggatccca tgcagatcgt gtccggaccc cttcatgagc
     1201 cgaaggttca ttttgaggcg ccgccttcac aagcgctcgc caaggaaatg gagcagtttg
     1261 tcggatggtt caatcgcagc gcgccggacg ggaaccgtcc cctgcctgcg ctcacccgcg
     1321 ccggcatcgc gcacttgtat tttgtcagca ttcatccgtt cgaggacggc aacgggcgga
     1381 tcgggcgggc gatcagcgag aaatcgctgt cgcaatcgct caaccaaccg acgctgatcg
     1441 cgctttcgca cgtcatcaat tcccgaaaaa aagcctatta caattcgctt gaacagagca
     1501 accggcacaa tgagatcacc ggctggctcg tggattttgc gcggacgatt ctggatgcgc
     1561 aaagttacac gcagcgacgg atcgatcgac tgatcgaaaa ggccaagttc ttcgaccgat
     1621 tccgggggca gttcaacgag cggcaggaga aggccatttc ccggatgttc aggggggagc
     1681 cggaaggatt cccgggaggc ctgagcgccg agaaatacat ccgcattacc ggcgcggcgc
     1741 gcgccacggc cactcgcgat ttgcaggatc tcgcggccaa aggagcgctg actcgaaccg
     1801 gcactctgaa aggcacgcgc tatcacctgg cgacgctccc caccggctga ctcgggcatt
     1861 gcaggcaaag ctttttcttc accctgcgtg accaggcact ttgcggagtc gattcgggtt
     1921 gccgcatcgc gtgaaagcgg aaatggccag gcaattccgc ccgcaacccc tgataaatat
     1981 acgctccgcg ggccagcgga tcacgtggcc gaatgcatct cggtcgtcat gtagcgaacc
     2041 gcgaaatcca cgcaggcccg gacccgggcg ggcaacgtcc ggtcgctcgg atggatcaca
     2101 tggacgggaa ggtccggggc gttccattgg ggcaggatcc ggacgaggcg gccggcgatc
     2161 aggtcttcgc gcaccagcca gtcgggcagc acggccacgc cggctccgcc ccgcacggct
     2221 tcccgcatgc tggtcacccc ttcgctaatc aagaggggtt cgatattcaa ttccttcccg
     2281 gccttcctcg gggcgctcaa gacaatcttg cgcgcgtcgc cgaacaatcc gccgcccagc
     2341 gagacccagg gccaggcctc cagatcctcg ggcgccgtcg cgactccctg ttttttcgcc
     2401 aatgcgggag cggcaaccag ataacgaacc accccgcccg cgggccgcgc caccacccgg
     2461 tcgtccgaga ttttccccac cacgactccc gcatcgaatc cctcgtcgat catgcgcgtc
     2521 agccggttcg aatatttcag ctccgcggtc acgcccgggt tcgccagtag gaaccgcgtg
     2581 atcaaccggg tgaccgcgga ctggccaaaa tcgaccgtgg cgaataatcg caggtggccg
     2641 cggatcgtgg tttgatcctc ccgcacccgc tgggccgcgc tctcgacgag ttccaaaatg
     2701 gcgcccgcat ccttgtaaag ccggctgccg gcctccgtga gattcatcgt gtgagtatcc
     2761 cgccggagca gcgacgcgcc gcagttctct tcgaggcgcc gcaaatgacg gctcagcgtg
     2821 ggctgcggca tcttcaagct gcgcgccgcc gccgagatgc ttccgctctc cacgatgcgg
     2881 acgaagaagc gaagcaggga aagatcttcc agtgcggtca ttcattcttt gaataaccga
     2941 tatggaaacc cggcggctag ccgaaaatca cgggcgggcg catgttctca ctcgtcacca
     3001 acttcatcga aacccattca accaaccact cacatgaata cctcaaaccc aaaagtcgcc
     3061 ctcatcaccg gttcctcccg cggaatcgga gccgccatcg ccaaacgcct cgcccaggat
     3121 ggcatgcggg tcgtgatcaa ttacgcaggc cgggcggccg acgcggaaaa ggtcgtgcag
     3181 gaaatcacga aagccggcgg ccgagccctc gccattcagg ccgatgtctc caacccggaa
     3241 gaagccgccc ggctcttcga gcaggccacc gcggagttcg gcggggtcga tatcctcgtc
     3301 aacaacgccg gcgtgatgca gccgggcctg gttccggttg cggaaaccag cgacgagctt
     3361 ttcgaccgcc tgatcgccat taatctgaaa ggcaccttca acaccttgcg cctcgcggca
     3421 aagcaactcc gcgccggtgg gcgcatcgtc aacttttcca ccagcgtcgt cggcctggcg
     3481 ctgccgggtt atgccgttta cgccagcacc aaggcggccg tcgaaaccct gacgaacatc
     3541 ttcgcgaagg aaatgcgcgg ccgccagatc accgtgaact ccgtggctcc cgggccgacc
     3601 gccaccgatc tctttctcaa cggaaagacc cccgagcaga tcgagcatct ggccaaaatg
     3661 ccgccgctcg agcgcctcgg ccgccccgag gacatcgcca acgtggtctc ccttctcgtg
     3721 ggagccgacg gcggctggat caacggccag acactccgcg ccaatggcgg catcgtctaa
     3781 ttccccacct cacgcgaaaa acttacctca atctcctatg aaatccacca ttctcatcac
     3841 c
//
LOCUS       DATDAY010000341         3132 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_477059, whole genome shotgun sequence.
ACCESSION   DATDAY010000341 DATDAY010000000
VERSION     DATDAY010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 3132)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3132)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3132
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_477059"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..233)
                     /locus_tag="VIS74_06780"
     CDS             complement(<1..233)
                     /locus_tag="VIS74_06780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792984.1"
                     /translation="MAKPKPRNRGRLDVAAAGAGPATVPTRWVKAVVGLFLLPVAWVL
                     TQTFFGTLAWAAVDRGFWAAEAFWFFALGAVIWG"
     gene            complement(305..862)
                     /gene="efp"
                     /locus_tag="VIS74_06785"
     CDS             complement(305..862)
                     /gene="efp"
                     /locus_tag="VIS74_06785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEY5792985.1"
                     /translation="MALANDLRKGMAIRYNGNPAIVLDIQHRTPGNLRAFVQAIVRYI
                     GTGKSADIRFSSTEKVDLVDVNRASLEFSYADHEGYHFMDPGTYETVTFQEAFVGGAK
                     DFLTENLKVEILYIEGRPAQIELPSSVNLKVTESAEGVRGDSANNVQKPATLETGKII
                     QVPLFIKEGELIKVDTREGTYMGRA"
     gene            complement(921..1892)
                     /locus_tag="VIS74_06790"
     CDS             complement(921..1892)
                     /locus_tag="VIS74_06790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006983645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HEY5792986.1"
                     /translation="MSLLEKARRVFEIELAETKAVAERLDESFVKAIELFLATIERGS
                     KIIVLGVGKSGHVGDKIAATMTSTGSPAVVLNSLNALHGDLGVVSDGDVVLALSYSGE
                     TEELLRILPALKRFDVRIVSITGKQRSTLAMASDVVLDVKVSQEACPMQLAPTSSSTA
                     MLVLGDALAMVLLEERGFTKEQFAKLHPGGSLGRALLLRVSEIMRDASITPLLTEATT
                     VLEAVQAMTQRKTGAAVVLKPDGTLAGIFTHGDFARHFPNHPDIGSRRVSEFMTAKPV
                     SIRSNHLAAEVLQVLEHHRVDDLVVLDESDRPIGLVDSQDLTRLKIL"
     gene            complement(1899..2576)
                     /locus_tag="VIS74_06795"
     CDS             complement(1899..2576)
                     /locus_tag="VIS74_06795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792987.1"
                     /translation="MAASLDITIRPYEPRDRDGVRKLCCETGFLGKPIDPVFEDRELF
                     ADYLTSYYTDWEPEASFVVEREGQIKGYLLGSRRPKRQQLHSLWMNLSLFARGMWRYA
                     GYRKATRDFIGWILRNSWKEVPAAPRKTAHFHFNLLPEVQSIAGTRQLLLAYFDYLRG
                     KGEKAVFGQVVTFEDRRGSKLFERYGFQVMERREITKYRNVDPRPVYLTTVIRQLDVP
                     SKIELAR"
     gene            complement(2584..>3132)
                     /locus_tag="VIS74_06800"
     CDS             complement(2584..>3132)
                     /locus_tag="VIS74_06800"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY5792988.1"
                     /translation="EGATLLLYVGRLASEKNVKTLFAAFELLHAAEAGKWHLLAIGDG
                     TQRGSLQALRERTGAVTWLPYCSEANQLAGYYRAADLFVHPGVCETFGLVTLESQASG
                     TPVVGIKGSYMDRIIFTNQSHWAAENTPEALAAAIRDNRRQDLRAAGLAASEMVRARY
                     SWRSVFDRLFGIYERVIAEYSP"
BASE COUNT          593 a         1024 c          923 g          592 t
ORIGIN      
        1 ccccagatga cggcccccag cgcgaaaaac caaaacgcct ccgcggccca gaacccgcga
       61 tccaccgccg cccacgccag cgtgccgaaa aaggtctgcg tgagcaccca ggccaccggc
      121 aggagaaaca ggcccacgac cgccttgacc caacgcgtgg gcacggtcgc cggaccggct
      181 ccggccgccg ccacgtccaa tctccccctg ttccggggtt tcggtttcgc catgcgggtg
      241 cttataacaa atcccggcgg gttcagcaac ggacggctcg gcgggattcc caaccgggga
      301 aaaatcaggc ccggcccatg taggtgccct cgcgggtatc caccttgatg agctcgcctt
      361 ccttgatgaa gagcggcacc tggatgatct tgccggtttc cagcgtggcg ggtttctgca
      421 cgttattggc cgagtctccg cgcacgcctt cggcggattc cgtcaccttg agattcaccg
      481 aagagggcag ttcgatctgc gccggacggc cttcgatgta gaggatttcc accttgaggt
      541 tttcggtgag gaaatccttc gcgccgccca cgaacgcttc ctggaaggtg accgtctcgt
      601 aggtcccggg gtccatgaag tgatacccct cgtggtcggc atagctgaac tccagcgagg
      661 cgcggttcac atcgaccagg tccaccttct cggtggagga aaagcggatg tcggcggatt
      721 tgccggtgcc gatgtagcgg acgatcgcct gcacgaacgc gcggaggttg ccgggggtgc
      781 ggtgctggat gtcgaggacg atggccggat tgccattata gcggattgcc atgcctttac
      841 ggagatcatt tgcgagggcc ataaaaatag aatcgttgga ttttgggtaa tccgccttgt
      901 tagctcagag cggggcggga ttaaaggatt ttcaatcggg tgaggtcctg cgagtccacg
      961 aggccgatcg gacgatccga ttcgtcgagc accacgagat catccaccct gtgatgttca
     1021 agaacttgca gcacttcggc cgcgaggtgg ttgctgcgga tgctgaccgg cttcgccgtc
     1081 atgaattcac tcacccggcg ggagccgatg tcgggatggt tcggaaaatg ccgcgcgaaa
     1141 tcgccgtggg taaagatgcc ggccagcgtg ccatccggct tcagcaccac ggcggctccc
     1201 gtttttcttt gggtcatggc ctgcaccgcc tccagcaccg ttgtcgcttc ggtcagcagc
     1261 ggagtgatgc tggcatcccg catgatttca ctcacccgca gcaacagcgc ccggccgagc
     1321 gagccgccgg gatgcagctt ggcgaattgc tccttggtga atcccctttc ctccagcagc
     1381 accatggcca gggcgtcccc gagaaccagc atcgccgtgg aactggaagt gggcgccagc
     1441 tgcatcgggc aggcttcctg ggacaccttc acgtccagca cgacatccga agccatcgcc
     1501 agggtcgaac gctgcttgcc ggtaatggag acgatccgca cgtcaaaccg cttcaacgcc
     1561 ggcaggatgc gcagcaactc ctcggtctcc ccgctgtagc tgagcgccag aaccacgtcg
     1621 ccgtcggaaa ccaccccgag gtcgccgtgc agcgcattga gggaattcag caccacggcg
     1681 ggcgagccgg tgctcgtcat cgtcgcggcg atcttgtcgc ccacgtgccc ggattttcca
     1741 acgcccagga caatgatctt gctgccgcgt tcgatggtcg ccagaaacaa ctcgatggcc
     1801 tttacgaaac tctcgtccag gcgctcggcc accgccttcg tctcggcgag ttcgatttca
     1861 aatacccggc gggctttttc cagaagactc atccgggatt aacgcgccag ctcgattttg
     1921 gaagggacgt ccagctgccg gatgaccgtc gttagataca ccggccgcgg atccacgttc
     1981 cggtatttcg tgatttcgcg gcgttccatc acctgaaacc cataacgctc aaagagtttg
     2041 gagccccggc ggtcctcgaa ggtcacgacc tggccaaaga cggccttttc ccctttgccg
     2101 cgcaggtagt caaaataggc cagcagcagc tgccgcgttc ccgcgatgct ctgcacttcc
     2161 ggcagcaggt taaaatggaa gtgcgccgtc ttgcggggcg cggcaggcac ctccttccag
     2221 gagtttcgca gaatccagcc aataaagtcg cgggtggcct tgcgatagcc cgcgtatcgc
     2281 cacattcccc gggcgaacag cgaaagattc atccaaaggc tgtgaagctg ctgccgcttc
     2341 gggcggcgcg aacccagcag ataccccttg atttgcccct cgcgctccac cacaaaactc
     2401 gcctccggct cccagtcggt gtaataactc gtcaggtaat ccgcaaacag ctcccggtcc
     2461 tcaaataccg gatcgatcgg tttgccgaga aaacccgtct cgcaacagag cttgcgaact
     2521 ccgtcccggt cccggggttc atacggccgg atggtgatgt cgagggatgc cgccatggtc
     2581 ttcttaagga ctgtattccg cgatcacccg ctcgtaaatg ccaaagagcc ggtcaaacac
     2641 cgaacgccag gagtagcgcg cccgcaccat ttcgctcgcg gccaatcccg ccgcgcgcag
     2701 atcctgccgc cggttgtccc ggatcgccgc cgccagggcc tccggcgtgt tctccgcggc
     2761 ccagtgggat tgattggtaa aaatgatgcg atccatgtag ctgcccttga tccccacgac
     2821 cggcgtgccg ctcgcctggc tttcgagcgt caccagtccg aacgtctcgc acacccccgg
     2881 atgcacgaac aaatcggcgg cacgatagta tccggccagc tggttcgcct ccgagcaata
     2941 cggcagccac gtcaccgcgc cggtccgctc ccgcaacgcc tgcagggagc cgcgctgggt
     3001 gccgtcgcca atggccagca gatgccattt tccggcctcc gccgcgtgca gcagctcgaa
     3061 ggcggcgaag agcgttttga catttttttc cgaagccagc ctcccgacgt agagcaggag
     3121 cgtggccccc tc
//
LOCUS       DATDAY010000342         2156 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_881572, whole genome shotgun sequence.
ACCESSION   DATDAY010000342 DATDAY010000000
VERSION     DATDAY010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2156)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2156)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2156
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_881572"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..424
                     /locus_tag="VIS74_06805"
     CDS             <1..424
                     /locus_tag="VIS74_06805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pilus assembly protein PilB"
                     /protein_id="HEY5792989.1"
                     /translation="TGAVTRLVDMGVEPFLISASLQCILAQRLIRKICKSCRSAYEPS
                     EAVLQQLGLSPFDIGDKNFYYGKGCDACNHTGYKGRKGIYELLKISDPLRELINERAP
                     GVVLRQKAIELGMTTLRADGLRSIYEGDSTIEEVIKYT"
     gene            complement(440..1429)
                     /gene="hemB"
                     /locus_tag="VIS74_06810"
     CDS             complement(440..1429)
                     /gene="hemB"
                     /locus_tag="VIS74_06810"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HEY5792990.1"
                     /translation="MSLNLSHRPRRSRRTAAIRQMVRETRLSVNDLIYPLFVKAAPEM
                     QAVQSMPGVNRHDCKSLVRECRAAAGLGIPAVALFPCIESAQKDATGSHSLADKNVLF
                     QAVRAVKKACPDLMVMTDIALDPYTSHGHDGVLAPDGRSVANDETVELLCQLAVREAE
                     AGADIVAPSDMMDGRVGAIRQALDEAGFAEVLILSYAAKYASAFYGPFREAVGSKIGK
                     DAISKSTYQMDPANVREAIREIQLDEDEGADWLMVKPAGPYLDVIRAVREASLLPLAA
                     YQVSGEYAQIQAAAQLGWLDYERTRDESLLAIKRAGADLIVTYFAREIAEALK"
     gene            1532..1607
                     /locus_tag="VIS74_06815"
     tRNA            1532..1607
                     /locus_tag="VIS74_06815"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1566..1568,aa:Glu,seq:ttc)
     gene            1762..1956
                     /locus_tag="VIS74_06820"
     CDS             1762..1956
                     /locus_tag="VIS74_06820"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY5792991.1"
                     /translation="MDDPLTIPEAARHLKIGVSTLRRYLRRRLLSHIVLPGNDYRIEV
                     SELEVFKKARTIPKNDKSKY"
     gene            1960..>2156
                     /locus_tag="VIS74_06825"
     CDS             1960..>2156
                     /locus_tag="VIS74_06825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792992.1"
                     /translation="MNNTTNQSDQNGVIATADEPQTETSVRFDFDLDELQGLMKALPR
                     VLSIPAITQHLQAVIELVPSYG"
BASE COUNT          424 a          699 c          616 g          417 t
ORIGIN      
        1 caccggcgcc gtcacccggc tggtggacat gggcgtcgag cccttcctca tctccgcgtc
       61 cctgcagtgc atcctcgccc agcgcctcat tcggaaaatc tgcaaatcct gccgcagcgc
      121 ctacgagccc agcgaggcgg tgctccagca gctggggctc tcccccttcg acatcggcga
      181 caagaatttt tactacggca agggctgcga cgcgtgcaac cacaccggct acaagggccg
      241 caagggcatc tacgaattgc tcaagatcag cgatccgctc cgcgaactca tcaacgaacg
      301 cgcgccgggc gtcgtgctcc gccagaaggc gatcgaactc gggatgacca cgctgcgcgc
      361 ggacggcctg cgcagcatct acgagggcga ctcgaccatc gaggaagtga tcaaatacac
      421 ctgatcccgc cgggatccgc tatttcagcg cctccgcgat ctcgcgggca aagtaggtca
      481 caatcaaatc cgcccccgcg cgcttgatcg ccagcagcga ttcgtcccgc gtgcgttcgt
      541 aatccagcca gcccaactgg gccgccgcct ggatttgcgc gtattcgccg gacacctgat
      601 acgccgcgag cggcagcaat gaggcctcgc gcacggcccg gatgacatcg agatacggcc
      661 ccgccggctt gaccatgagc cagtccgcgc cctcgtcctc gtcgagctgg atttcgcgga
      721 tcgcctcgcg gacgtttgcc ggatccattt ggtaagtgga tttgctgatt gcgtccttcc
      781 cgatcttgct gccgaccgcc tcccggaacg ggccgtaaaa cgcggacgca tatttggccg
      841 cgtaggacag gatcagcacc tccgcaaaac ccgcctcgtc cagcgcctgc cggatcgccc
      901 ccacgcgtcc atccatcatg tccgacggcg ccacgatgtc cgcccccgcc tcggcttcgc
      961 ggacggccag ctggcagagc agctccacgg tctcgtcatt ggccacgctg cggccgtcgg
     1021 gcgccagcac gccgtcgtgg ccgtggctgg tgtagggatc cagcgcgatg tccgtcatca
     1081 ccattaagtc cgggcaggcc tttttgactg cgcgcaccgc ctgaaaaagc acgttcttgt
     1141 ccgcgagcga gtgcgagccg gtggcatcct tttgggcgga ttcgatgcac gggaaaaggg
     1201 cgaccgcggg aatgcccagc cccgcggcgg cgcggcactc gcgcaccagg gatttgcaat
     1261 cgtgccggtt gacgccgggc atggattgga ccgcctgcat ttccggcgcc gctttgacga
     1321 agaggggata aatcaaatca ttcaccgaga ggcgggtttc ccgcaccatc tggcggatcg
     1381 cggcggttcg gcggctgcgg cgggggcgat gagagagatt cagactcatg gggttagcat
     1441 cgctttgcgg ccggttcggg tcaagaacgc caattttccc ttgcatccct gcgccggtcc
     1501 gcgcatattc accggctctc tttcaaacag agaccctatc gtccagtggc ctaggacacc
     1561 gccctttcac ggcggtaaca cgggttcgaa tcccgttagg gtcgccattt ttctctctga
     1621 agatggcgat ttactctgta ggccaaaaac cgttggtgat cgttggtgat catttttggc
     1681 cgttgaaggc cgttttccgt cagggaaaat cagggaaaga ttttttggaa ttgtcaccag
     1741 aatgatctca aaaccggaaa cgtggatgac ccgctcacga ttccggaagc cgcccgacac
     1801 ctcaaaattg gtgtcagcac gcttcgtcgg tatctgcggc gccgcctcct gagtcacatc
     1861 gtccttccgg gcaacgacta tcgcatcgag gtctcggaac tcgaagtatt caaaaaggcg
     1921 cggaccatcc cgaaaaacga caaatcaaaa tactgaccta tgaacaatac taccaaccaa
     1981 tcggatcaga atggcgtaat cgccactgct gatgaaccac aaacggaaac ctctgtccgg
     2041 ttcgacttcg accttgatga gctgcagggc ttgatgaagg cgctccctcg cgtcctgtcg
     2101 attccggcga tcactcaaca tcttcaagcc gtcatcgagc ttgtgccgag ctatgg
//
LOCUS       DATDAY010000343         3378 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_1007122, whole genome shotgun sequence.
ACCESSION   DATDAY010000343 DATDAY010000000
VERSION     DATDAY010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 3378)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3378)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3378
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1007122"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..546)
                     /locus_tag="VIS74_06830"
     CDS             complement(<1..546)
                     /locus_tag="VIS74_06830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792993.1"
                     /translation="MTAFPRFLLLILFAAATSWTMAGAPSPAQPPAQPESAKAPVVDA
                     RSFVRVIATNQNYDFLRPWIKRPPYSRRGLGTVLADGRILVTAELVLRNAYVELEKID
                     SGEQCPAEVAVIDYQANLALLRPGRPDFLKEARPLELDSGATVGDRAEVIQLEPNGSI
                     ATTSAILTTITVANYPIDEMGL"
     gene            complement(554..2008)
                     /locus_tag="VIS74_06835"
     CDS             complement(554..2008)
                     /locus_tag="VIS74_06835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEY5792994.1"
                     /translation="MQRVWIAGSLALLAATLPGIAAPGEITGSVVRIEATTQDANYKV
                     PWSAGQMSGGVGAGFIIDGKRILTNAHVVSNSRFLTVSKQGDPKPYTARVQHIAHDCD
                     LALLQIDDPHFFEGTKPLSFGGIPEIESTVSVFGYPIGGDRLSVTRGIVSRVDFQPYS
                     HSGVDSHLTIQIDAAINPGNSGGPVLQAGKVVGVAFQGYSGDVAQNVGYMIPAPVINR
                     FLADVKDGSYDHYMDLALSYFPLFNPAARQALGLPDDGAGVLVGEVYGGGSADGIIHQ
                     GDVLLAVDGNPIASDGSVELEGGTVEMAEIVERKLKGDKVALQILRKGQKMDVTVPLN
                     ERWPFGLQANTYDVRPRFITFGGLIFQPVDQNFMNEHNPGNLRLRYLFDHFITDQVYR
                     ERPELVALSGILPDPTNSYTSDFVYSIVDRINDHKIQRLEDVRKAFAEPVEYYVIHLL
                     GEGRPIVLERKEVEKARSRIRLRYGVTREENLNS"
     gene            2093..>3378
                     /gene="lysS"
                     /locus_tag="VIS74_06840"
     CDS             2093..>3378
                     /gene="lysS"
                     /locus_tag="VIS74_06840"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEY5792995.1"
                     /translation="MSAEHSPLQEIIAFRRQKLDALKAAGVDPFGQRFENAGDLAGIR
                     AAFAEGQPVRAAGRVTAHRDMGKSQFLDLSDVTGRMQVFLHAKEIGEEAWQLFKQVDL
                     GDFVGVEGEYFVTRTGEPTIRAQKVTVLAKSLRPLPDKWHGVSDTETKYRQRYLDLIA
                     NPESREVFLKRIRIVREIRRFLEDRGFVEVETPMMQAVAGGAAAQPFKTHHNALGQDL
                     YLRIAPELYLKRLLVGGFPKLFELNRNFRNEGVSRRHNPEFTMLEAYWAYADFEAMAD
                     LVEELVCHLALTVAGSLQIGHRNAEGQPARTINLARPWRRARYEDLIRGVVPDWFELD
                     LERKRARCAGLGLDISNCGEEFEITQQIFEKCVEEKTNDPLFVTHLPVQLVPLARQNR
                     ENPAQVDVYELVINGQEISPGYSELNDPDVQRQRLM"
BASE COUNT          630 a         1024 c         1094 g          630 t
ORIGIN      
        1 caggcccatc tcgtcgatcg ggtaattcgc gaccgtgatc gtggtcagga tcgcgctggt
       61 ggtggcgatg ctgccattgg gttcgagctg gatgacttcc gcgcggtcgc cgacggttgc
      121 gcccgaatcc agctccaatg gccgggcttc cttgagaaaa tccggccgcc cgggccggag
      181 cagcgcgaga ttggcctgat aatcgatcac cgcgacctcg gcggggcatt gttcgccgga
      241 gtcgattttt tccagctcca cgtaagcgtt ccgcagcacc agctcggcgg tgacgaggat
      301 gcgcccatcc gccagcacgg ttcccagccc gcgccggctg tagggcgggc gtttgatcca
      361 gggacgcaga aagtcgtagt tctggttcgt ggcaatgacg cggacaaagg accgggcgtc
      421 cacgacgggg gccttggcgc tttccggctg ggccggcggt tgagccggcg aaggggcgcc
      481 cgccatcgtc cagctggtgg cggcggcaaa caggatcaat aataaaaagc ggggaaacgc
      541 ggtcatggcg gaattaggag ttcagatttt cctcgcgggt gacgccgtag cgcaggcgga
      601 tgcgtgagcg ggccttctcg acctcttttc gctccagcac gatcggccgg ccttcgccca
      661 gcaaatgaat gacataatat tccaccggct cggcaaaggc cttccgcaca tcttccagcc
      721 gttgaatctt gtgatcattg atccggtcca cgatcgaata cacgaaatcg ctcgtgtagg
      781 aattggtggg gtccggcagg atgccgctga gcgcgaccag ctcgggccgt tcccggtaaa
      841 cctgatcggt gatgaagtga tcgaagaggt agcgcagccg caggtttccg ggattgtgct
      901 cattcatgaa gttctgatcc acgggctgga agatcaggcc gccgaaggtg atgaagcggg
      961 ggcggacatc gtaggtgttg gcctgcaatc caaacggcca gcgttcgttg agcggcacgg
     1021 tgacatccat tttctgcccc ttgcgcagga tttgcagcgc caccttgtcg cccttgagct
     1081 tgcgctcgac gatttccgcc atttccacgg tgccgccctc gagctccacc gagccgtcgc
     1141 tcgcgatcgg gttgccatcg accgcgagca gcacgtcgcc ctggtgaatg atgccatccg
     1201 ccgatccgcc gccgtaaact tccccgacca gcacgccggc gccgtcgtcc ggcaggccca
     1261 gcgcctggcg ggcggcggga ttgaaaagcg ggaaataact cagggccaga tccatgtagt
     1321 ggtcatacga accgtccttc acgtcggcga ggaagcggtt gatgaccgga gcgggaatca
     1381 tgtaaccgac gttctgggcc acgtcgccgc tgtagccctg gaaggccacg ccgacgacct
     1441 tgccggcctg caggacgggg ccgccgctgt tgccgggatt gatggcggca tcgatctgga
     1501 tcgtcaaatg cgagtccact ccggaatggc tgtagggctg gaaatccacg cgggacacaa
     1561 tgccgcgcgt cacggagagc cggtcgccgc cgatggggta gccgaaaacc gaaaccgtgg
     1621 attcgatctc ggggatgccg ccgaagctca ggggtttggt gccctcgaaa aaatgcgggt
     1681 catcaatttg cagcagggcg aggtcgcaat catgcgcgat gtgttggacc cgggcggtgt
     1741 agggcttggg atcgccctgc ttggagacgg tcagaaagcg ggaattgctg acgacatggg
     1801 cgttggtcag gatgcgtttg ccgtcaatga tgaaccccgc gcccacgccg ccgctcattt
     1861 gccccgccga ccacggaacc ttgtagttgg catcctgggt ggtggcttcg attctcacca
     1921 cgcttccggt gatttcgccc ggggcggcga tcccggggag agttgccgcc agcagagcca
     1981 gcgaacctgc gatccaaacg cgttgcatcc gcacaaccta tgcaaggcaa cgtggctgac
     2041 aacttattct tgaaagcggc ggggattggc ggcacggttg gcatcttttc ccatgtcagc
     2101 cgagcattca cccctgcaag aaatcatcgc cttccgccgt caaaaacttg acgcgctgaa
     2161 agccgcgggc gtggatccct tcggccagcg gtttgaaaac gcgggcgacc tcgccggcat
     2221 ccgcgccgcc ttcgcggaag gacagccggt ccgcgcggcc ggccgggtga ccgcccatcg
     2281 cgacatgggc aagagccagt ttttggacct ttccgacgtg accggccgca tgcaggtctt
     2341 cctgcacgcc aaggaaatcg gggaggaggc ctggcagctt ttcaagcagg tggatctggg
     2401 cgattttgtc ggggtggagg gggaatattt cgtcacccgc accggcgagc ccaccattcg
     2461 cgcccaaaaa gtcacggtcc tggcgaaatc cctccgcccg ctgcccgaca agtggcatgg
     2521 cgtgagcgac acggaaacca aataccgcca gcgttacctc gacttgatcg ccaacccgga
     2581 atcccgcgaa gtgttcctca agcgcatccg catcgtccgc gaaatccgcc gctttttgga
     2641 ggaccggggg tttgtcgagg tggagacgcc gatgatgcag gccgtcgcgg gcggcgccgc
     2701 cgcgcagccc ttcaagaccc atcacaacgc gctggggcag gatttgtatc tgcgcatcgc
     2761 gccggagctt tacctcaagc gcctgctcgt ggggggattc cccaagcttt tcgaactcaa
     2821 ccgcaatttt cgcaacgagg gcgtctcccg ccggcacaat ccggagttta cgatgctcga
     2881 agcctactgg gcctacgcgg acttcgaggc gatggcggat ttggtggagg aactcgtctg
     2941 ccacctcgcg ttaaccgtgg ccggttcgct ccagatcggg caccggaacg ccgagggcca
     3001 gcccgccagg acgatcaatc tcgcccgtcc gtggcggcgc gcgcggtatg aggatttgat
     3061 ccgcggtgtc gtgccggatt ggttcgaatt ggatttggag cgaaagcggg cgcgctgcgc
     3121 tgggctcggg ctggatattt ccaactgcgg cgaggagttt gagatcaccc agcagatttt
     3181 cgagaagtgc gtggaggaaa aaaccaatga cccgctcttc gtgacccatc tgccggtcca
     3241 gctcgtgccg ctcgcgcgcc agaaccggga aaaccccgcg caggtggacg tttacgaact
     3301 cgtcatcaac ggccaggaaa tttcgccggg ctactcggag ttgaacgatc ccgacgtcca
     3361 gcgccagcgg ttgatgga
//
LOCUS       DATDAY010000344         2943 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_1008639, whole genome shotgun sequence.
ACCESSION   DATDAY010000344 DATDAY010000000
VERSION     DATDAY010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2943)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2943)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2943
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1008639"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..597
                     /locus_tag="VIS74_06845"
     CDS             <1..597
                     /locus_tag="VIS74_06845"
                     /inference="COORDINATES: protein motif:HMM:NF013717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HEY5792996.1"
                     /translation="ESDESLLATALRQAGVPLTFAELAEAGTVEPQPEPYVQFPDCRF
                     PTPSGKIEIASAAAEREGLPRLPFPHADSRPAPGRLRVLSPAGPYRMNSEYGNAPALL
                     ARAGLDEVRLHPREAARRSLSDGGEVELRNEAGHLALRVRLDPAVPEGTALVHKGRWP
                     KLSANRANINALFTGKKSDMGESTTVHSVEAELRAVGT"
     gene            complement(619..1155)
                     /locus_tag="VIS74_06850"
     CDS             complement(619..1155)
                     /locus_tag="VIS74_06850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792997.1"
                     /translation="MNALKSVISGESWKTLFAVVFGTWMAIWVYAVLHNQYLIRIAPE
                     HFTVWHYKMPFFTSYTMLGIAYAFAASFSPGMFLGICLYVSGRLFNRPKFSPKELIFS
                     TFWVWIAVEICSPAAGFWVWHFKAGIYPDWVYPDNSLGLLITQSIQITAYLSGAAFSI
                     LLIVLTWRQRKHLARRLS"
     gene            complement(1276..1470)
                     /locus_tag="VIS74_06855"
     CDS             complement(1276..1470)
                     /locus_tag="VIS74_06855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792998.1"
                     /translation="MKNFPYTQYEGTLLWEALANALEKLKENRDIVIATADPYVIGYL
                     AKAITQALPSSIQPEVLRKQ"
     gene            2111..2317
                     /locus_tag="VIS74_06860"
     CDS             2111..2317
                     /locus_tag="VIS74_06860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5792999.1"
                     /translation="MTQSLEHQFAIYRNHLDEVKRSHPGLVEFCDQEWRAVHSTPPDA
                     DEWLTLKRLRESSDRVHDRALAGS"
     gene            2379..2588
                     /locus_tag="VIS74_06865"
     CDS             2379..2588
                     /locus_tag="VIS74_06865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5793000.1"
                     /translation="MAADAQEIAKLIHDKIAQNEAAATLTAGMLAIELERESHPRAAE
                     AKQVSDILSQFSRDLSDLIKKLRET"
     gene            2607..>2943
                     /locus_tag="VIS74_06870"
     CDS             2607..>2943
                     /locus_tag="VIS74_06870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5793001.1"
                     /translation="MDGNALISNGFFYAGIGLFLGFLLRSRRFREVNILSATAESLAL
                     IRIVVSLVMLGYVIGENYPSIALIPREYLAPQGVIRWLFHAPFFREWHADPIFLQALT
                     GVTVFFLVAA"
BASE COUNT          662 a          833 c          859 g          589 t
ORIGIN      
        1 gaatcggacg agtccctcct cgccaccgcg ctgcggcagg cgggagtccc gctgaccttt
       61 gccgagctgg ccgaagcggg cacggtggag ccgcagcccg agccctatgt ccaatttccg
      121 gactgccgtt ttcccacgcc gagcggaaaa atcgagatcg ccagcgcggc ggcggagcgc
      181 gagggcctgc cgcggctgcc ctttccccac gccgattccc gcccggcgcc gggccggctg
      241 cgcgtgcttt cccccgccgg tccctaccgc atgaacagcg aatacggcaa cgctcccgcg
      301 ctgcttgccc gggccgggct cgacgaggtt cggctgcatc cccgcgaggc cgcccgccgg
      361 tccctgtccg acggcggcga ggtcgaactc cgcaacgaag cgggccatct ggccctgcgc
      421 gtgcggctcg atcccgccgt gcccgaggga accgcgctcg tgcacaaggg ccgctggccg
      481 aaactctcgg ccaaccgcgc gaacatcaac gccctgttca ccggaaaaaa gtccgacatg
      541 ggcgagagca ccacggtgca ttccgtcgaa gccgaactgc gggcggtggg gacgtaatca
      601 agggaatgga ggcggccatc agctcaagcg cctggccagg tgtttccttt ggcgccaggt
      661 aagaacaatc aggaggattg aaaaagccgc cccggaaaga taagcggtaa tttgaatgct
      721 ttgggtaatg agcaatccaa gcgagttatc tggataaacc caatccggat aaattcccgc
      781 cttgaaatgc caaacccaaa atccggcagc gggcgagcaa atctcaacgg cgatccagac
      841 ccaaaaggtg ctaaaaataa gttcttttgg gctgaattta ggccggttga ataagcggcc
      901 cgaaacatac aggcaaattc ccaggaacat gcccggtgag aaggaggccg caaaagcata
      961 agcgatgcct aacatcgtat agctggtgaa aaatggcatc ttgtaatgcc acacggtaaa
     1021 atgctccgga gcaatgcgga tgaggtattg gttatgaagc accgcgtaaa cccaaatggc
     1081 catccaagtt ccaaagacga ccgcgaataa cgtcttccaa gattcgccgg aaataaccga
     1141 tttaagcgcg ttcacgcaat ttataaacct gacatctggc gtcagttacc accggtttct
     1201 gcgcaaacaa aaaccatgac gccgcaaaac ggggcatgaa ggcggttggc tggacgcgat
     1261 ggttcagctt taagctcatt gtttcctcaa aacctcaggt tgaatagaag atggcagtgc
     1321 ctgggtgatt gctttggcta gataaccgat aacgtatgga tccgctgtag caatcacgat
     1381 atcacggttt tctttcaact tttccagtgc gttcgcgaga gcctcccata acagagttcc
     1441 ttcgtattgt gtataaggga agttcttcat tttatttcgc cgaacattct attaaattgg
     1501 aattagtata atcctgaatg gatggaaaac cggttgtcat gccgatcggg aaaaccgggc
     1561 attggctgat tgagttccgg tgaagaagaa tcatccgcag attacgccga tttccgcgga
     1621 taaagccggg tttgtttaaa aaaatctgcg tccatcggtg aaatctgcgg attaaaagcc
     1681 ctcggtcact tcctgtggtt gggcggtaga tttagccgcc ggcagccgtg tcggcaagat
     1741 aaaaggtttg ggagcaagtc attcggaggt tggaggggca gtcgtgccag cgaactgaaa
     1801 tcgacggacc gaccaatcga tcggtccctg cccaggttgg gcgggttcga tgagccgagc
     1861 gcaaggcgag ccagccttgc cgcgggcaag cgcagcgacg gcaacccgcc atcgctcgtt
     1921 tcgccagatt ggcgctcatc taaaaaatca tctcacgcaa agacgcgacg caaagcaaag
     1981 cagcagtcct tcccggagaa cctttgcgcc ttggcgtctt tgcgtgcgac ccaatgcgat
     2041 ggtgcatcca ttgctaaaat cacctccgca acaagcgcgg cggctcgtcc gggagcgatc
     2101 cgattacgtg atgactcaat ctctggaaca tcaattcgcg atctaccgga atcatctgga
     2161 tgaggtgaag cgaagccatc ccggcttggt ggagttctgc gatcaggaat ggcgggcggt
     2221 ccattccact cccccggatg cggacgagtg gttaaccctc aaacggctgc gggaatcctc
     2281 cgaccgggtg catgaccggg ctctcgccgg atcctgaggc ggggcggggg cggaaaatat
     2341 ccgtgcttcg caggtgaccc gtgagttata ctcgcgggat ggccgctgat gcacaggaga
     2401 ttgccaaact catccacgac aagattgccc aaaacgaagc cgccgccacg ctgacggcgg
     2461 ggatgctggc aatcgaactc gagcgcgaaa gccatccccg cgccgccgag gccaagcagg
     2521 tttccgacat tctttcgcag ttttcccgcg atctcagcga tttgatcaaa aaattgcggg
     2581 agacataacg ccatccgcgg gagagaatgg acggaaacgc gctgatctcg aacgggttct
     2641 tttacgcggg catcgggctg tttttaggat ttctgctgcg ttcgcggcgg tttcgggagg
     2701 tcaacatcct cagcgccacc gcggagtcgc tggccttgat ccgcatcgtc gtctcgctgg
     2761 tgatgctggg gtatgtgatt ggcgaaaatt acccgagcat tgccttgatt ccgcgggaat
     2821 acctggcgcc ccagggggtg atccgatggc tgtttcacgc gcccttcttc cgggaatggc
     2881 atgcggatcc gatctttttg caggccctca cgggggtcac cgtctttttt ctggtcgccg
     2941 ccg
//
LOCUS       DATDAY010000345         2309 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_127735, whole genome shotgun sequence.
ACCESSION   DATDAY010000345 DATDAY010000000
VERSION     DATDAY010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2309)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2309)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2309
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_127735"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..666)
                     /locus_tag="VIS74_06875"
     CDS             complement(<1..666)
                     /locus_tag="VIS74_06875"
                     /inference="COORDINATES: protein motif:HMM:NF018484.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chlorite dismutase family protein"
                     /protein_id="HEY5793002.1"
                     /translation="MKAVPSSPVLPAEGRHVLHLFYRIDRSVWTYFSEEEKWEGRSRL
                     VSVIEEIRAQPETQLLLLSGVTPKSDVGFMLLTPDLQFADEASKRLTASLGPEVLTPV
                     FSWLSLTERSEYTTTEEEYTATLAPEDNVEEKRAAFRARMEKYGKDRLYPNLPDWPVV
                     CFYPMSKRREAGQNWYSLDFAARKALMQGHARVGRTYSGRVLQLITGSTGLDDGEWGV
                     TLFA"
     gene            738..971
                     /locus_tag="VIS74_06880"
     CDS             738..971
                     /locus_tag="VIS74_06880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HEY5793003.1"
                     /translation="MADLSNKYPGNVGGRYYVDDQCIDCDLCRETAPSSFKRDDDGGH
                     SFVWKQPETPEEEALCKEALEGCPVEAIGNDGE"
     gene            1036..1389
                     /locus_tag="VIS74_06885"
     CDS             1036..1389
                     /locus_tag="VIS74_06885"
                     /inference="COORDINATES: protein motif:HMM:NF017103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HEY5793004.1"
                     /translation="MKRSFRSFVLSEWRGLPQPDGEPDRCVPISEVLKQILPKLGLKD
                     RLDEQEIQDAWHEIVGDFLANHAKPSGLKHGVLLIQVIQASVRYELETVWKAKILLRL
                     QERFGAKKIREVKFR"
     gene            1493..2107
                     /locus_tag="VIS74_06890"
     CDS             1493..2107
                     /locus_tag="VIS74_06890"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEY5793005.1"
                     /translation="MIRNLIFDWSGTLADDLPPVLVATNAIFREYGQRDLTLEEFRRH
                     FRLPFSEFYAELLPEATLEGLEPLYERFFAGLQDDVELLPGARELLEFCRRTERRTFL
                     LSSIKATHFEVQAANLGVRDLFEHAYVQVMDKREKIREILSIHQLDPAETLFIGDMVH
                     DIETARHGGVLGVAITTGYDPVEKLLPARPAVIVDSLNSLRAFL"
     gene            2104..>2309
                     /locus_tag="VIS74_06895"
     CDS             2104..>2309
                     /locus_tag="VIS74_06895"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: protein motif:HMM:NF014233.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HEY5793006.1"
                     /translation="MTDQILIRGLEVFARIGITPEERAEPQRLRLDVEIEPAAPFAEL
                     DEQISRTVDYYRASLRLAALAGSR"
BASE COUNT          484 a          690 c          684 g          451 t
ORIGIN      
        1 cgcaaagagc gtgacgcccc attcgccgtc gtcgagcccg gtcgagccgg taatcagctg
       61 cagcacccgg ccggaatagg tccgccccac ccgcgcgtgc ccctgcatga gggccttgcg
      121 ggcggcaaag tcgagcgaat accagttctg tcccgcctct cggcgcttgg acatgggata
      181 aaaacacacc accggccagt ccgggaggtt tggatacaaa cgatccttgc cgtatttctc
      241 cattcgcgcg cggaaggccg cccgtttttc ctccacgtta tcctccggcg cgagcgttgc
      301 cgtgtattcc tcctcggtcg tggtgtattc gctgcgctcg gtcagcgaca gccagctgaa
      361 aacgggggtc aacacctccg gcccgaggct ggcggtcagg cgcttgctgg cctcgtcggc
      421 aaactgcagg tcgggcgtca gcagcatgaa tccgacatcg gatttcggcg taaccccgct
      481 caaaagcaga agttgcgtct ccggctgggc gcggatttcc tcgatgacgg acaccagccg
      541 gctgcggccc tcccattttt cctcctccga aaaataggtc cagaccgagc ggtcgatgcg
      601 ataaaaaagg tggagaacgt gacggccctc ggcgggcaaa acgggcgatg atggaacggc
      661 tttcactctt tcactttgcg accctgaaaa aacaattgca atcccgttga aagatcgtag
      721 ctttccgcac tgaaaacatg gctgacttaa gcaacaaata tccgggaaac gtcggtggac
      781 gctactacgt ggatgatcaa tgcatcgatt gcgatctgtg ccgggaaacc gcgcccagca
      841 gcttcaagcg cgacgatgac ggcggtcatt ccttcgtttg gaaacaaccc gagacacccg
      901 aggaagaagc gctctgcaag gaagcactgg aaggttgccc ggtcgaagcg atcggcaacg
      961 acggcgaata aggccagcgg ccgaaccgac cgtttaaact tccgctttgc cggcagcgag
     1021 gtaacttgtc ggctcatgaa acggtcattt cgcagttttg tcctgtcgga atggcggggt
     1081 ctcccccagc ccgacggcga gccggatcgc tgcgtgccga tttccgaagt cctcaagcaa
     1141 atcctgccca agctcggtct caaggaccgg ctggatgagc aggaaatcca ggacgcctgg
     1201 cacgaaatcg tcggcgactt cctcgccaac catgccaagc ccagcggttt gaagcatggc
     1261 gtgctgctca ttcaagtcat ccaggcctcc gtgcgctacg agctggaaac cgtctggaag
     1321 gccaaaatcc tcctgcgcct gcaggaacgc ttcggcgcga agaaaatccg cgaggtgaag
     1381 ttccgctgaa gcggagtttt aagttggtaa gtcttaagtt ttaagtcaaa tgcactccga
     1441 gaaggcggtc ttcccttaaa acttacaaac ttaagactta aaactttctt ccatgatccg
     1501 aaacctcatc ttcgactggt ccggcacgct ggcggacgac ctgcctccgg tgctcgtcgc
     1561 cacaaacgcc atttttcggg aatacggcca gcgcgatctc acgctggagg aattccgccg
     1621 gcatttccgg ctgccgtttt cggaatttta cgcggaatta ttgcccgaag cgaccctgga
     1681 ggggctggag cccttgtatg aacggttttt tgccgggctg caggatgacg tggaactgct
     1741 gccgggcgcc cgggaactgc tggagttttg ccggcgaacg gagcggcgca cgtttttgct
     1801 gagctccatc aaggccacgc actttgaagt tcaggccgcc aacctcggcg tgcgggatct
     1861 gtttgaacac gcctacgtgc aggtcatgga caaacgcgaa aaaatccgcg aaatcctcag
     1921 cattcaccag ctcgatcccg cggaaacgct gttcattggc gacatggtgc acgacatcga
     1981 aaccgcgcgg catggcggcg tgctgggcgt ggcgatcacc acggggtatg acccggtcga
     2041 aaaactgctg cccgcgcgcc cggcggtgat cgtggacagc ctgaattccc tccgcgcctt
     2101 tttatgaccg atcaaattct gatccggggg ctggaggttt tcgcgcggat tggcattacc
     2161 cccgaagagc gcgcggaacc gcagcggctg cgcctggatg tggagatcga gcccgcggcg
     2221 ccgttcgcgg agctggacga acaaatcagc cggaccgtgg attattaccg ggcaagcctg
     2281 cggctggcgg cattggccgg cagccggga
//
LOCUS       DATDAY010000346         6184 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_2247338, whole genome shotgun sequence.
ACCESSION   DATDAY010000346 DATDAY010000000
VERSION     DATDAY010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 6184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6184)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6184
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2247338"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..979)
                     /locus_tag="VIS74_06900"
     CDS             complement(<1..979)
                     /locus_tag="VIS74_06900"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: protein motif:HMM:NF013279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEY5793007.1"
                     /translation="MHPLLRKLLGMNWLLFGLMLALMGYGIYAIYSATWFREATFWRS
                     QLLWMLVCLPGFFAFSFIDYRWIRWGALPIYLVSVGLLLATRIIGVERYGAKSWLDLG
                     PLNFQPSQTAIVGGILVLALFLSQFRNLPAILRIIGCAIIAGAPCLLILVQPDLGSVL
                     VWGPVILAMFFIAGIPKRYLIVMLLCVIIAIPVAVNFVLKPYQRDRIIAFLDHDVDPL
                     GVSWTINQSLIAIGSGGTAGKGFKAPNTQNELGFLPSTIVHNDFIFSVMGEQHGFIGA
                     VILITVFAFLLLAGLQIAMSAEDDLGRLVAVGIVAMLFAHIFMNIGMTIS"
     gene            complement(1104..1895)
                     /locus_tag="VIS74_06905"
     CDS             complement(1104..1895)
                     /locus_tag="VIS74_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY5793008.1"
                     /translation="MPLLEVTDLKVWFPVLGGVFRRPVGNIKAVDGVSFSVEAGQTVG
                     LVGESGSGKTTVGRALLKLVRATSGRVSFAGRDLLPMSEREFRPLRKEVQMIFQDPFG
                     SLNPRMTVGAIIAEPLQIHFPKLTAADRAERVAALLRQVNLKPEMMRRYPHEFSGGQR
                     QRIGIARALAVEPRFIVCDEPVSALDVSVQAQIVNLLRDLQDELGVAYLFIAHDLAVV
                     EHLSDFVLVMHHGKIVEAASAAEIYENPQDDYTKSLLAAVPSLGI"
     gene            complement(1937..2416)
                     /locus_tag="VIS74_06910"
     CDS             complement(1937..2416)
                     /locus_tag="VIS74_06910"
                     /inference="COORDINATES: protein motif:HMM:NF012579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEY5793009.1"
                     /translation="MERLQRMISIAEILTPAQVDLKVEAATKDEAVLKVLDLLRGDPR
                     VQNWERLRRAVQERNAPAVEQNECGILIAHGRTNSLSGLVMAAGRLLQPLTCPEIAVP
                     IRLVFVAGIPRAVDTEYLRVIGAIVRALRDRHQLERLLGVKEAARFVTLLSSQETRL"
     gene            complement(2395..3234)
                     /locus_tag="VIS74_06915"
     CDS             complement(2395..3234)
                     /locus_tag="VIS74_06915"
                     /inference="COORDINATES: protein motif:HMM:NF039482.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HEY5793010.1"
                     /translation="MTGLIAHTEKPGAAELVRAVAAEFSRRGAELLFEAETAQVAGFS
                     STLSERDLAARCDLLLVLGGDGSILRLLHRLEGNSPAIFGINLGSLGFLTCLGATEYA
                     EAVDSILARNYVHSPRTLLEVRHGGKALLALNDVVISRGERSRLVKTRVRIDGQTLTE
                     YNADGLIISTPTGSTAYSLSSGGPILMPESGAFVVTPICPHVLTNRSVVVSDESVIEA
                     ELLVEGQQVFATVDGQAFFEIQVGERLILQKSSRRLELAMLPGRSFSDVLRQKLKWSG
                     SNV"
     gene            complement(3231..3989)
                     /locus_tag="VIS74_06920"
     CDS             complement(3231..3989)
                     /locus_tag="VIS74_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HEY5793011.1"
                     /translation="MKQSLHSNKIPKLRLDQLLVERGLCASREKAQRLILAGSVRVHA
                     APAAKPGQLVPADAVVELLAAERYVGRGGTKLEAALAHFQVPVQGAVCADLGASTGGF
                     TDCLLQHGAARVHAIDVGRGQLDWKLRQDPRVIVREGINARRLTVADLGERVALAVAD
                     VSFISLALILPAAFDLLVPGGRMIVLIKPQFELTKSQVGKGGIVRDEALQLSAVEKIR
                     GVAEQALGRRWGGFIPSPILGAKGNREFLALLET"
     gene            4035..4295
                     /locus_tag="VIS74_06925"
     CDS             4035..4295
                     /locus_tag="VIS74_06925"
                     /inference="COORDINATES: protein motif:HMM:NF012759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HEY5793012.1"
                     /translation="MGDDELKSQIRQVMIEHLMLKLTPEEIGSTTPLFSPEGLALDSI
                     DALELAVAIEKHFGVSIPNAETARGAFQNVDSLAAFIQKNRA"
     gene            4401..4892
                     /locus_tag="VIS74_06930"
     CDS             4401..4892
                     /locus_tag="VIS74_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEY5793013.1"
                     /translation="MKLQTLKDLYIYELKDLYSAEKQIIRALPKMAKAAANEKVAGDF
                     NEHLEQTKTHVNRLEDILSSLHQSTRGPKCKGMEGVLEEGAEMIEEEADDEVRDAGLI
                     ASAQRVEHYEIAGYGTARTYAQLIGDHKGAKLLQTTLNEEEETDRKLTALAESSINVA
                     ALK"
     gene            5103..5570
                     /locus_tag="VIS74_06935"
     CDS             5103..5570
                     /locus_tag="VIS74_06935"
                     /inference="COORDINATES: protein motif:HMM:NF037928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host attachment protein"
                     /protein_id="HEY5793014.1"
                     /translation="MIATRDPRMTQVNFVFVADRGQLKTYLLDRNHSPNLRLVNALDL
                     VESRRPVSAEFTAPAGRFPNNGTGGQGNSSAERGGVGRERQARATRTVARQIAEVLGQ
                     IRPRAWYLAAPSEIYAELLSHVPMEFQASLAGKIEKDLVNSPPEQILQYVHPT"
     gene            5572..5829
                     /locus_tag="VIS74_06940"
     CDS             5572..5829
                     /locus_tag="VIS74_06940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5793015.1"
                     /translation="MQSEPLILFPPTHEQSTPCFHVEGSLADSFLKYLRSEGLRAWEP
                     PEKLEKRGPDSRRVVEIKIEADTPEDYLEGLLRKFLIGHPA"
     gene            5829..>6184
                     /locus_tag="VIS74_06945"
     CDS             5829..>6184
                     /locus_tag="VIS74_06945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5793016.1"
                     /translation="MNFDSPAFLAPALQIGAGVILLLMGRRLFWIFVGVVGFLVGFQF
                     GTAAFAGSPWIWLLAVAVGLAACLLAIVLQKVAVVLAGAAAGWLLGLRLDGRVNRHQP
                     RGQDKRFGNKPFGERA"
BASE COUNT         1286 a         2018 c         1789 g         1091 t
ORIGIN      
        1 cggagatggt catgccgata ttcatgaaaa tatgcgcaaa cagcatggcg acaatgccca
       61 ccgccaccag cctcccgagg tcgtcctccg cgctcatggc gatctgcagc cccgccagca
      121 gcaggaacgc aaacaccgtg atgagaatca ccgcgccgat gaaaccgtgc tgctcgccca
      181 tgacggaaaa aataaagtca ttgtgcacga tcgtgctggg cagaaacccc agctcgttct
      241 gggtgttcgg cgccttgaag cccttgccgg ccgtgccgcc gctgccaatg gcgatcaggg
      301 actgattgat cgtccagctg acgccgaggg gatcgacgtc atgatcgaga aacgcgatga
      361 ttcgatcccg ctggtagggt ttcagcacaa aattaaccgc cacgggtatg gcgatgatca
      421 cgcacagcag catcacgatg aggtaacgct tcggaattcc cgcgatgaaa aacatcgcca
      481 gaatcaccgg cccccatacg agaaccgagc ccagatccgg ctggaccaga atcagcaggc
      541 acggcgcccc cgcgataatc gcgcagccga tgatccgcag gatggccgga agattgcgga
      601 actggctcag gaacaaggcc agcaccagga tgcccccgac aatcgccgtt tgcgagggct
      661 gaaaattcag cggccccaga tccagccagc ttttcgcgcc gtagcgctcg acgccgatga
      721 tgcgcgtggc cagcagcagg cccacggaaa ccagataaat gggcagcgcc ccccagcgaa
      781 tccagcggta atcgatgaag gaaaacgcga agaaacccgg caggcagacc agcatccaga
      841 gcagctggct gcgccagaac gtggcttcgc ggaaccaggt cgcgctgtaa atggcgtaga
      901 tgccataacc catcaaggcc agcatcagcc caaagaggag ccaattcatg ccgagcaatt
      961 tgcgaaggag cggatgcatc aggaaatttt cgattctcga ttttcgattc tcgattgcca
     1021 aacggcggcg cttccttcca atcgagaatc gaaaatcgaa aatcgaaaat ggccgcgggc
     1081 tccgtttttt tccagcgcgg atttcatatt ccgaggctcg gcaccgccgc cagcagggac
     1141 ttcgtgtaat cgtcctgcgg attttcgtag atttccgccg cggaggcggc ttcgacgatt
     1201 tttccgtggt gcatcaccag cacaaaatcg ctgaggtgct cgaccaccgc caagtcgtgc
     1261 gcgatgaaga gataagccac ccccagctcg tcctggagat cccgcagcag attcacgatc
     1321 tgcgcctgca cgctgacatc cagcgcgctc accggctcgt cgcagacaat gaaccgcggc
     1381 tccacggcca gggctcgggc gatgccgatg cgctggcgct ggccgccgct gaactcgtgc
     1441 ggatagcgcc gcatcatttc cggcttgaga ttcacctgcc gcaacagcgc ggccacccgt
     1501 tccgcgcgat ccgcggcggt caatttcgga aaatgaatct gcagcggctc ggcaatgatc
     1561 gctcccaccg tcatgcgggg attcaacgac ccgaacggat cctgaaaaat catctgcact
     1621 tccttgcgga gcgggcggaa ttcgcgctcg ctcatcggca gcagatcgcg cccggcaaag
     1681 ctcaccctgc ccgaggtggc gcgcaccagt ttcagcagcg cgcggccgac cgtggtcttg
     1741 ccgctgccgc tttcgcccac caggccgacg gtctggccgg cctcgacgga aaagctcacg
     1801 ccatccaccg ccttgatgtt cccgaccggc cggcggaaaa cgcctccgag cacggggaac
     1861 cacactttca gatcggtaac ttccagcaac ggcatcgctc aagtttgagg cgggtggaaa
     1921 gaaaatcaag ccccgctcac aggcgagttt cctggctgct gagcagcgtc acgaaacgcg
     1981 cggcttcctt cacccccagc aggcgctcca gctgatgccg gtcccgcaag gcgcggacga
     2041 tcgctccgat cacgcgaagg tattccgtat ccaccgcccg gggaatgccg gcgacaaaca
     2101 ccagccggat gggcacggcg atctccgggc aggtcagggg ctgcagcaat ctccccgccg
     2161 ccatgaccag gccggagagg ctgttcgtgc ggccgtgggc gattaagatg ccgcattcgt
     2221 tttgttccac cgcgggagcg ttccgttcct ggaccgcccg gcggaggcgc tcccagtttt
     2281 gcacgcgcgg atcgccccgc aggagatcga gcacttttaa cacggcttca tccttggtcg
     2341 cggcctccac cttcagatcc acctgagccg gcgtgagaat ctccgcgatg ctgatcatac
     2401 gttggagccg ctccatttga gcttctggcg aagcacgtcc gaaaaactgc ggcccgggag
     2461 catggccagc tccagccgcc gggaggattt ttgcagaatc agccgctccc ccacctggat
     2521 ttcaaaaaac gcctgcccgt ccaccgtggc aaatacctgc tgcccctcga ccagcaactc
     2581 ggcttcgatg accgactcgt cgctcaccac cacggaacga ttggtgagga catgcgggca
     2641 gatcggcgtg acgacaaacg cgcccgactc gggcatcaag atcggcccgc ccgaggaaag
     2701 cgagtaggcg gtggagccgg tcggggtcga gatgatcaag ccgtccgcgt tatactcggt
     2761 gagcgtctgc ccgtcgatgc gcacgcgggt cttgaccagg cgggaacgct cgccgcggct
     2821 gatcaccaca tcgttaagcg ccaggagggc cttgccgcca tggcgcactt ccagcagcgt
     2881 gcgcgggctg tgcacgtaat tccgcgcgag aatgctgtcc acggcctccg cgtattccgt
     2941 agcgcccagg caggtcagga acccgagcga accgaggttg atcccgaaaa tcgcgggcga
     3001 gtttccctcc aaacgatgca ggagccggag aatggagcca tcccccccca gcacgagcag
     3061 cagatcgcaa cgggccgcca gatcccgttc ggaaagcgtg ctggaaaacc cggccacctg
     3121 ggccgtttcc gcttcgaaga gcaattcggc gccgcgccgg gaaaattccg ccgccacggc
     3181 ccgcaccagc tccgccgcgc ccggcttctc ggtatgcgca atcagcccgg tcatgtttcc
     3241 aaaagcgcca gaaattcccg attccccttc gcgcccagaa tcggcgaggg aataaatccg
     3301 ccccagcgcc gtcccagcgc ctgctcggcc acgccgcgga ttttttcgac ggcggaaagc
     3361 tgcagggctt catcccggac gatgcccccc tttcccacct gggatttggt caactcaaat
     3421 tgtggtttga tgagcacgat catacgtccg ccgggcacga gtaagtcaaa ggcggcgggt
     3481 aaaatcaatg ccagggaaat aaaactcacg tcggccacgg cgagcgccac ccgctccccc
     3541 aaatccgcca ccgtcaaacg ccgcgcgttg attccttccc ggacgatcac gcgcggatcc
     3601 tgccgcagtt tccagtcgag ctggccccgc cccacatcga tggcgtgcac ccgggcggcg
     3661 ccgtgctgta gcaggcaatc cgtgaatccc ccggtcgaag cgccgagatc tgcgcagacg
     3721 gctccctgga ccggaacctg aaaatgggcc agcgccgctt cgagcttggt tccgccgcgc
     3781 cccacgtaac gctccgccgc cagcaattcc accaccgcgt ccgccggcac cagctgcccg
     3841 ggcttggcgg cgggcgccgc gtggacgcgc acgcttcccg ccaggatcaa ccgctgggcc
     3901 ttttcgcgcg aggcgcataa cccgcgctcc accaaaagtt gatccagccg cagctttggg
     3961 attttatttg aatgcagact ttgtttcact gagcgcccct ccctttcatc ctttcatagc
     4021 agataccaac caccatgggc gacgacgaac tcaaatccca aatccggcag gtcatgatcg
     4081 agcacctcat gctcaaattg actcccgaag aaatcggctc caccaccccg ctgttcagcc
     4141 cggaaggcct ggccctggac tcgatcgacg ccctggaact cgccgtggcg atcgaaaaac
     4201 acttcggcgt ttccattccc aatgccgaaa ccgcccgcgg ggcctttcaa aacgtggatt
     4261 ccctcgccgc gttcattcag aaaaatcggg cctgaggcgg cttccgagcg aggaatgtcc
     4321 ctgattttgg gtcagggggc cggatgcagc catccggcat tgctggatgt tgcgatcctc
     4381 ccttgaaacc caccccattc atgaaactcc aaaccctgaa agatctctac atttacgagc
     4441 tcaaggatct ctacagcgcg gaaaaacaaa tcattcgcgc ccttccgaag atggcgaagg
     4501 ccgccgccaa tgaaaaagtg gcgggggact ttaacgagca ccttgagcag accaaaacgc
     4561 atgtcaatcg cctggaggat atcctctcca gccttcacca gagcacccgc ggcccgaaat
     4621 gcaaaggcat ggaaggcgtg ctggaggaag gcgcggagat gatcgaggag gaagccgacg
     4681 atgaagttcg cgatgcgggc ttgatcgctt ccgcccagcg cgtcgaacat tacgagattg
     4741 ccggctacgg aaccgcccgc acttacgccc agctgatcgg cgatcacaag ggagccaaac
     4801 tgctgcaaac cactctcaat gaagaggagg agacggatcg gaagctcacc gcattggccg
     4861 agtcatccat caacgtcgcg gcgcttaaat agggcgattc caagaaaccg gcagccgcaa
     4921 aagcgcgcag aggtctcaaa gaaggcgtgc aacccggtga gggttgcggc ttttttgcgc
     4981 tctctgtgtt cccttgcggc catgactttt gttaattcac ccgaaagcca aacggcccct
     5041 gatttttcaa tctggaaaca cgcgtttctc cccggagccg cgatcccttt ttgagattgc
     5101 ttatgattgc cacccgagat ccccgcatga cccaggtgaa cttcgttttc gtggcggatc
     5161 gggggcagtt aaaaacgtat ctcctcgacc ggaatcactc ccccaacctg cgcttggtga
     5221 acgcgctcga tctcgtggag agccgccgcc ccgtttccgc ggaattcacc gcgcccgccg
     5281 gccggtttcc caacaatggc accggcggcc agggaaattc ctccgccgaa cgcggaggag
     5341 tgggccggga gcggcaagcc cgcgccaccc ggaccgtcgc ccggcagatc gcggaggtgt
     5401 tgggccagat tcgtccccgg gcctggtatc tggcggcgcc ctcggaaatt tatgccgagc
     5461 tgctgagcca tgtgccgatg gaattccagg catcgctggc cggaaagatc gaaaaggatc
     5521 tcgtcaattc acccccggag caaatcctcc aatacgttca ccccacctga tatgcaatcc
     5581 gaacccctca tcctgtttcc gccgacccac gagcagtcca ccccctgctt tcacgtcgaa
     5641 ggcagcctcg cggattcctt tttgaaatat ctccgcagcg aaggccttcg ggcttgggaa
     5701 ccgccggaga agctcgaaaa gcggggcccc gattcccgcc gcgtggtcga gattaaaatc
     5761 gaggcggaca cccccgagga ttacctggag ggtctgctcc ggaaattcct catcggccat
     5821 cccgcttgat gaacttcgat tcaccggcat ttctggctcc ggcgctgcaa atcggcgccg
     5881 gggtgatcct tctcctgatg gggcggcgct tgttttggat cttcgtcggc gtggtcgggt
     5941 ttttggtggg gttccagttc ggaaccgcgg ctttcgccgg ttcgccatgg atctggctgc
     6001 tggcggtcgc ggtggggttg gccgcgtgcc tcctggcaat cgtgctgcaa aaggtcgccg
     6061 tcgtcctcgc cggggcggcg gccggctggc tgctgggcct gcggctggac ggtagagtta
     6121 accgccatca gccgcgcggg caagacaaaa ggtttggaaa caagccattt ggggaaaggg
     6181 cgga
//
LOCUS       DATDAY010000347         3195 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_2423373, whole genome shotgun sequence.
ACCESSION   DATDAY010000347 DATDAY010000000
VERSION     DATDAY010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 3195)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3195)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3195
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2423373"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1117
                     /locus_tag="VIS74_06950"
     CDS             <1..1117
                     /locus_tag="VIS74_06950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5793017.1"
                     /translation="KRAEMEGLKTQISRHAKLLAGALRSQYEILATQENNLRNQLNSI
                     RNQIDEFTQTAAQLSQSMGDPVEMGKLLKESPDGPAGLANVIVVVDRATPVFKPVRPK
                     LWLNLTLGVLFGGAAGLGIAALRAVLDTRLKSAQQVERQTGKPCLALLPNLESLTRRN
                     RSQKVFDHPEYPIGLGYLRTHLLHPGRKPGGEKIIGLTPAQPFLNCSNLVADLGILLA
                     QAEKKTLILDLHLKTPRIASIYGMQIQHELNDWFISQEPLKACINYSAVQELAVLSNR
                     KSNRTDLNDMLSRRLLTHALIELLDEWDFILIDAPNILTDWNLMLALPEHSPLLVIAE
                     YGRTTADNALQTLAHARGPRWDVEGIILANAPRRLTR"
     gene            complement(1148..2596)
                     /locus_tag="VIS74_06955"
     CDS             complement(1148..2596)
                     /locus_tag="VIS74_06955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5793018.1"
                     /translation="MASATRRAYLQELFWNQLWQSANFAAKAGFLILLTPLMLWKWGA
                     AGYGLFALASSLLVSMALLDGGVRALTRLRLADAHRRNDDAGFARAYGEGMLTFACAC
                     LVAVAAAFLLAGTGGVNRWLNLPAGGEWVLALTAAMTAVFMLTSLALEPLAAKGNLSA
                     LKAANTWGALAAIPFTAAAVALGGSVLLAIGIYFACLTLPNFALAARAGIFPLLRRKG
                     IFRFDPGVIWQTLRAGFWYYLTTVSLVIKTHGLTFVVAAIAGPAEAGMFYILLRLTEI
                     VGNVGATASETSLAALAAARDADERRENFRQSWLYTGIFCLHGALVMALLGEPLLRFW
                     LPEKELVPAGAGWAMAVFGLAGAFSRVVVNAGMGLHLVKPAALGNALEALADLAGAVA
                     GYHLAGLPGLFVGGSIGIGFLLGPAWKIAGACGAAAGALYLASLGRLVPGLLIAAAAQ
                     AVSVWLFGLPGWLAALGISGAAALGQIRRLHR"
     gene            2655..>3195
                     /locus_tag="VIS74_06960"
     CDS             2655..>3195
                     /locus_tag="VIS74_06960"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEY5793019.1"
                     /translation="MAITRRKFILKAAAATLAGASAGYALWEAHDLLTRPRDLLIPGL
                     PAGFSGIRAAFVADLHHGPYASLEYLGGMVRRIHEAAPELVLLGGDYTYYKARYIPPV
                     MEILSALRAPLGVYAVRGNHDNRAGKSLSSLELRRNGIREITNTGLWLERGGDRLYLA
                     GVDDLWTGRPDLRQALAPVS"
BASE COUNT          623 a         1168 c          930 g          474 t
ORIGIN      
        1 caagcgggcg gaaatggaag ggctgaaaac ccaaatctcc cgccacgcca agctgctggc
       61 cggcgcgctg cgttcgcaat acgagatcct ggccacccag gaaaacaacc tccgcaacca
      121 gctgaactcg atccgcaacc agatcgatga attcacccag accgccgccc agctgtccca
      181 gagcatggga gatccggtgg aaatgggcaa actgctcaag gaatccccgg atggcccggc
      241 gggcctggcc aacgtcatcg tcgtggtgga ccgcgccacg ccggtcttca agccggtccg
      301 gcccaaactc tggctgaacc tcaccctggg cgttcttttc ggcggggcgg cggggttggg
      361 gatcgccgcg ctgcgcgccg tgctcgatac gcggctcaaa tccgcccagc aagtcgaacg
      421 ccagaccggg aaaccctgcc tcgccctgct gccgaacctg gaatccctca ccaggcgcaa
      481 tcgctcccag aaagttttcg accatccgga atatcccatc gggctcggtt atctccgcac
      541 ccatttattg catcccggac ggaagccggg cggggagaaa atcatcggcc tcactcccgc
      601 ccagccgttc ctgaactgct ccaatctcgt cgccgacctc ggcatccttc tcgcccaggc
      661 cgagaaaaag accctcatcc tcgatcttca cctcaaaact ccccgcatcg cctccattta
      721 cgggatgcaa atccagcacg agctcaacga ctggttcatc tcccaggaac cgttgaaggc
      781 ctgcatcaac tactccgccg tccaggagct ggcggtgctt tccaatcgaa aatccaaccg
      841 caccgatctc aacgacatgc tttcgcggcg gctgttgacc cacgcgttga tcgaactgct
      901 cgacgagtgg gattttatcc tgatcgacgc gcccaatatt ttgaccgatt ggaatctgat
      961 gctcgcgctg ccggagcaca gcccgcttct ggtcattgcg gaatatggcc gcaccaccgc
     1021 ggacaatgcc ctgcaaaccc tcgcgcacgc gcgcgggccg cgctgggatg tcgagggcat
     1081 catcctggcc aacgcccccc gccgcctgac gcgatagtcg ggcgctaacc acgcgatcat
     1141 caaaacttca ccggtgaagc cgccggatct gccccagggc ggccgccccc gaaatcccca
     1201 gggcggccag ccacccgggc agtccgaaaa gccaaaccga gacggcctga gccgccgcgg
     1261 cgatcagcaa cccggggacc aggcgcccca gcgaggcgag gtagagagcg cccgccgccg
     1321 cgccgcaggc tcccgcaatt ttccaggccg gacccagcaa aaaaccgatg ccgatgctgc
     1381 cgcccacgaa caggccgggc aagccggcga gatggtagcc cgccaccgcc ccggcaagat
     1441 ccgccagggc ttccagcgca ttgccgaggg ccgccggctt caccaggtgc aatcccattc
     1501 ccgcgttcac caccacgcgg ctgaacgccc ccgcgagccc gaagaccgcc atggcccagc
     1561 ccgcgccggc gggaaccagc tccttctccg gcagccagaa ccggagcagc ggctcgccca
     1621 gcagcgccat gaccagcgcc ccgtgcaggc aaaaaattcc cgtgtagagc cagctttggc
     1681 ggaagttctc gcgccgctcg tccgcatccc gcgccgccgc cagggccgcc agcgaggttt
     1741 cgctggcggt ggcccccacg tttccgacaa tctccgtcag ccgcagcagg atgtagaaca
     1801 tgcccgcctc ggcgggaccg gcgatggcgg ccaccacgaa ggtcagccca tgggttttga
     1861 tcaccaggga aaccgtggtc aggtaatacc agaagcccgc gcgcagggtt tgccagatca
     1921 cccccggatc gaaccgaaaa atccccttcc gccgcagcag gggaaaaatg cccgcccgcg
     1981 ccgccagcgc gaaattcggc agcgtgaggc aggcgaaata aattccgatc gccagcagga
     2041 ccgaaccgcc cagcgccacc gccgcggccg taaagggaat ggccgccagc gcgccccagg
     2101 tattcgccgc cttgagcgcg gacaggttcc ccttcgccgc caggggctcc aacgccagcg
     2161 aggtgagcat aaaaaccgcc gtcatcgccg cggtgagcgc cagcacccat tcccctcccg
     2221 ccggcaggtt cagccaccgg ttcaccccgc ccgtccccgc caggaggaag gccgccgcca
     2281 ccgcaaccag gcaggcgcac gcaaacgtga gcatcccctc gccgtaagcg cgggcaaagc
     2341 cggcgtcatc gttccggcgg tgcgcatcgg ccagccgcag ccgcgtcaag gcccgcacgc
     2401 ccccgtcgag cagcgccatg gaaaccagca gcgaactggc cagcgcaaag agcccgtaac
     2461 ccgccgcgcc ccatttccag agcatcaacg gagtcaggag aatcaggaaa cccgccttcg
     2521 ccgcgaaatt ggcgctttgc cagagctgat tccagaacaa ctcttgcagg taggcgcggc
     2581 gggtcgcgga agccattggc cgatttttat agggcgaaaa tccggcgcgt gctatatgga
     2641 ttttcgggag attcatggcc atcacgcgtc gaaaattcat cctgaaggcg gccgcggcta
     2701 ccctcgccgg cgcctcggcc ggttacgcgt tgtgggaagc gcatgacctt ctcaccaggc
     2761 cccgggacct cctgattccc gggctgccgg cgggcttttc cggcatccgg gcggccttcg
     2821 tcgcggacct ccaccacggc ccctacgcct cgctggaata cctcggcggc atggtgcgcc
     2881 gcatccacga agccgctccc gaacttgtgc tgctgggcgg cgattacact tattacaagg
     2941 cccgctacat tcccccggtc atggaaatcc tttccgcgct gcgggcgccc ctcggggtct
     3001 atgcggtgcg gggaaatcat gacaaccgcg ccggcaagtc gctttcctcc ctggagctga
     3061 ggaggaatgg cattcgcgaa atcaccaata ccggcctctg gctggaacgc gggggcgacc
     3121 ggctgtatct cgccggagtg gatgacctct ggaccggccg ccccgatctc cggcaggcgc
     3181 tcgccccggt ctccg
//
LOCUS       DATDAY010000348         2644 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_834014, whole genome shotgun sequence.
ACCESSION   DATDAY010000348 DATDAY010000000
VERSION     DATDAY010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2644)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2644)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2644
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_834014"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1077
                     /locus_tag="VIS74_06965"
     CDS             <1..1077
                     /locus_tag="VIS74_06965"
                     /inference="COORDINATES: protein motif:HMM:NF012588.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase"
                     /protein_id="HEY5793020.1"
                     /translation="ASKPVLMRDSLTPDRFARNIENFIGTVELPIGLAGPLRVNGVSA
                     AGDYYVPLATTEAALVASYTRGCLLISESGGCASIVLAEGVSRAPGFIFESMHLAGEF
                     IAWALTQREAFSREANATTRHGKLVEMRVTVEGNHVYLNFEYLTGDAAGQNMVTIATQ
                     AICNYIRLNSPIQPRHSFVEANLSGDKKASSQSFQSVRGKKVSAEAVIPAHLLEKRLR
                     TSATTMEEYWRMSALGGVQSGTIGVQGHYANGLAALFIATGQDAACVSEAAVGITRFE
                     ARNDGSLYAAVTLPNLIVGTVGGGTSLPAQSACLDILGLRGNGHARALAEVAAALALA
                     GELSIIGALCAGHFARAHQKLARA"
     gene            1074..1994
                     /locus_tag="VIS74_06970"
     CDS             1074..1994
                     /locus_tag="VIS74_06970"
                     /inference="COORDINATES: protein motif:HMM:NF013226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HEY5793021.1"
                     /translation="MKGEPNRWWIYQRERFPILAHGPLILAFSLSSVCFSSLLRQDER
                     FPALTPILVAFFCSFFSFLHLRLADEFKDFEEDSRFRPYRPVPRGLVTLRQLGGVWAA
                     TAGIQLLLTLALDWRLAAPLGITWIYLALMSREFFCRTWLKNHPFTYMWTHMLIMPLI
                     DFFATACDWLPATGHPPAGLFWFVAASFFNGFIIEIGRKIRAPEDEENGVETYSFLWG
                     RSRAAGAWWLAMGLTLICALFAAAQIHFLLPAALGLGMAFLFALLNGVRFLRDPKPGR
                     GKSFELIAGIWTLLLYLILGVVPMAWRIFS"
     gene            1991..>2644
                     /locus_tag="VIS74_06975"
     CDS             1991..>2644
                     /locus_tag="VIS74_06975"
                     /inference="COORDINATES: protein motif:HMM:NF013491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HEY5793022.1"
                     /translation="MNWILDWRAAGFSSFPVGGKAQALGSLSAGGFPIPRWVVIPPSA
                     FDACLSANQRNLLNQAARAAQAREILQDLAPASELMEAVRVYLATHFPNTDSFAVRSS
                     ALDEDGAQASFAGQLESHLNVTRETLAARIADVWRSGFSERIFLYRQQAGLANPPTAP
                     AVIIQEMIPADSAGVAFSADPVTGRRGAAVVASVFGLGEALVSGEKNSDLFEVDRGGK
                     "
BASE COUNT          442 a          807 c          816 g          579 t
ORIGIN      
        1 gcgtcgaaac cggttttaat gcgtgattcc ctcacgccgg atcgatttgc caggaacatc
       61 gaaaatttca tcggcacggt ggaactcccg atcggcctgg ccggcccgct gcgcgtcaat
      121 ggcgtttcgg cggcgggcga ctattacgtg cccctggcca ccacggaagc cgccctggtc
      181 gcgtcctata cccgggggtg cctattgatc agcgaaagcg gaggctgcgc cagcatcgtg
      241 ctcgcggaag gcgtgagccg cgcccctggc ttcatctttg aaagcatgca tctggccggt
      301 gaattcattg cctgggcgct cactcagcgg gaggcctttt cccgcgaggc caatgcaacc
      361 acccggcacg gcaaattggt ggagatgcgg gtgaccgtcg aaggcaacca cgtttacctc
      421 aattttgaat acctgaccgg tgacgccgcc ggccaaaaca tggtgaccat cgccacccag
      481 gcgatctgca attacatccg gctgaactcg ccgattcagc cgcggcattc cttcgtcgaa
      541 gcgaatttgt cgggagacaa aaaggccagc tcccaatcct tccagtcggt ccgcggcaaa
      601 aaggtctccg ccgaggccgt cattcccgct catttgctgg aaaagcggct ccgcacctcg
      661 gcaacaacca tggaggaata ttggagaatg tcggcgctgg gcggcgtgca aagcggcacg
      721 atcggcgtgc agggccatta cgccaacggg ctcgcggcgc tcttcatcgc cacggggcag
      781 gacgccgcct gcgtgtccga ggccgcggtg ggcatcaccc gttttgaagc ccgaaatgat
      841 ggcagcctgt atgccgcggt caccttgccg aatttgatcg tgggaaccgt gggcggcggc
      901 acctcattgc cggcccaatc ggcttgcctg gacattcttg gactgagagg aaatggccat
      961 gcccgcgccc tggcggaagt cgcggcggca ctggcgctcg cgggggaact ttccatcatc
     1021 ggcgcactgt gtgcggggca ttttgcccgg gctcaccaga aacttgcgag agcgtgaagg
     1081 gcgaacccaa ccgctggtgg atttatcaac gggaacgttt ccccattttg gcgcacgggc
     1141 cgctgattct ggccttcagc ctttcttcag tctgcttttc ttccctgctg cggcaggacg
     1201 agcgcttccc cgcattgacc ccaatcctgg tcgcgttttt ctgctcattc ttctcgtttc
     1261 tgcacctgcg gctggcggac gaattcaagg atttcgagga agactcccgc ttccgcccct
     1321 accggccggt gccgcggggg ctcgtcacgc tccggcaatt gggcggggtt tgggccgcca
     1381 ccgccggaat ccagcttctt ctgaccctcg cgctggattg gcggctcgcc gccccgctgg
     1441 gaatcacctg gatttatctg gccttgatga gccgggaatt cttttgccgg acatggctca
     1501 agaatcatcc gttcacctac atgtggaccc acatgttgat catgccgttg atcgattttt
     1561 tcgccacggc ttgcgattgg ctgcccgcaa ccggacatcc gcccgccggg ctcttttggt
     1621 ttgtggcggc cagctttttt aacggattca tcatcgagat cggccgaaaa atccgcgcgc
     1681 cggaagatga ggagaacggc gtggaaacct attcgtttct ctggggccgg agccgcgcgg
     1741 ccggggcctg gtggctggcc atgggcttga cgctgatctg cgcgcttttc gccgcggcgc
     1801 agattcattt tctgctgccc gccgcccttg ggctgggcat ggcctttctc tttgcgttgt
     1861 tgaatggcgt gcgttttctc cgagatccga agccgggacg cggaaagtcg tttgagttaa
     1921 tcgccggaat ctggacgctg ttgctgtatc tgattctcgg ggtggttccg atggcctgga
     1981 ggattttttc atgaactgga tcctggattg gcgcgcggcg ggtttttcca gtttccccgt
     2041 ggggggcaag gcgcaggcgc tgggcagttt gagtgcgggt gggtttccca ttccgcgttg
     2101 ggtggtgatt cctccatcag cgtttgatgc ctgtctttcc gccaaccagc gaaaccttct
     2161 caaccaggcc gcccgcgccg cgcaggcgcg ggaaattctg caagacctgg ctccggcttc
     2221 cgagctgatg gaagcggtcc gggtttacct ggccacccat tttccgaaca ccgattcctt
     2281 cgccgtccgt tcttccgcgc tcgatgaaga tggggctcag gcttcctttg ccggccagtt
     2341 ggaaagccat ttgaacgtca ctcgcgaaac gctggccgca cgcatcgccg acgtgtggag
     2401 atcgggattc agcgagcgga tttttttata ccgccagcag gccggccttg ccaatccgcc
     2461 aaccgcgccc gccgtgatca ttcaggaaat gattcccgcg gacagcgccg gagtcgcctt
     2521 tagcgccgat ccggtcacgg gccggcgggg agctgccgtg gtcgcttcgg tattcgggct
     2581 cggcgaagcc ctggtctccg gggaaaaaaa cagcgatctc ttcgaagtgg atcgcggggg
     2641 aaaa
//
LOCUS       DATDAY010000349         2088 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_2073570, whole genome shotgun sequence.
ACCESSION   DATDAY010000349 DATDAY010000000
VERSION     DATDAY010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2088)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2088)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2088
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2073570"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(9..1277)
                     /gene="nusA"
                     /locus_tag="VIS74_06980"
     CDS             complement(9..1277)
                     /gene="nusA"
                     /locus_tag="VIS74_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HEY5793023.1"
                     /translation="MNSELIAMLDYLERERGIKREVLVEAISSALLAASKKSFTAGTR
                     ELRIEINPKNGQIKAFAKLIAAESITNPHDEILLSKARLTQPEVQPGEEVEVEVTPKD
                     FGRIAAQTARQAITQRLRQVEKNMIYDEFKDRAGEIVSGTVRRFERSDVIIDLGKFEA
                     VMPNKERVSTEEYSVGDRIRAYVVAVENNQRGPEIILSRSHPNFIRRLFELEVSEIAD
                     RTVELRGIAREAGYRTKVAVYSADDKVDPVGACVGVRGARVKNIVRELNNEKVDIIRW
                     YADPKEFVKEALKPARIRSIYLDEKTKTIRIAVDKEDLSVAIGKRGQNARLSAKLTGW
                     EIDIQEDKSAEQALAAQKAQAANSLSEALGISDEEAGVLAAGGMNSVEVIAMCEASDI
                     AGVLGVDTERAEKILEAARRFDPKAVLPAT"
     gene            complement(1378..>2088)
                     /locus_tag="VIS74_06985"
     CDS             complement(1378..>2088)
                     /locus_tag="VIS74_06985"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY5793024.1"
                     /translation="PITSPITGQIFFHEALLRLFLPGADAPARAAAFIQRISAAGLSY
                     DVDRFVLQKSCEAMREYPDLILSANLHASSLCNLNLIDTVEQLLASHGIGASRLILEV
                     TETEPISNFEQARTIMTRLRLMGCRFALDDFGAGFSPLRYLKTLPVDLLKIDGFFIHD
                     LSDEPYNQAILRSIQEISHYAGIQTVAEKVENERELAAAVSLGVDYVQGFLLAKPRAT
                     PYRPEEIEFAFPAAAALK"
BASE COUNT          389 a          594 c          661 g          444 t
ORIGIN      
        1 aggaaaacct atgtggcggg taagacggct ttgggatcga agcggcgggc ggcttcgagg
       61 attttttcgg cgcgctcagt gtccacgccc aacacgccgg cgatgtcgct ggcttcacac
      121 atcgcgatga cttcgacgga attcattccg cccgcggcga gcacgcccgc ttcttcgtcg
      181 ctgatgccca gagcttcgga aagcgaattg gcggcctggg ccttctgggc ggcaagcgcc
      241 tgctcggcgg acttgtcttc ctggatgtca atttcccagc cggtgagttt ggcggaaagg
      301 cgggcattct ggccgcgctt gccgatggcc acggagagat cttccttgtc cacggcgatc
      361 cggatggtct tggttttttc gtcgagataa atggagcgga tgcgcgcggg tttgagcgct
      421 tcctttacaa attccttggg atccgcatac cagcggatga tgtccacctt ctcgttgttg
      481 agctcgcgga cgatgttctt gacgcgggcg ccgcgcacgc cgacgcaggc gccgaccggg
      541 tccaccttgt cgtccgcgct gtaaacggcg accttggtgc ggtagccggc ctcgcgggca
      601 atgccgcgaa gctcgaccgt gcgatcggca atttcgctga cctcgagctc gaagaggcgg
      661 cggatgaaat tcggatggct gcgggaaaga atgatttccg ggccgcgctg gttgttttcc
      721 accgcgacga cataggcgcg gatgcgatcc cccacgctgt attcctcggt ggaaacgcgc
      781 tccttgttcg gcatgacggc ctcgaatttc ccgaggtcaa tgatcacgtc cgaacgctcg
      841 aaccggcgga cggttccgct gacgatctcg ccggcccgat ccttgaactc gtcgtagatc
      901 atattcttct cgacctggcg caggcgctgg gtgatcgcct ggcgggccgt ctgcgcggcg
      961 atgcggccaa aatccttcgg cgtgacttcg acctccactt cctcgcccgg ctgcacttcg
     1021 ggctgggtca agcgggcctt gctcagcagg atttcatcat gcggattcgt aatgctttcg
     1081 gcggcaatca gtttcgcgaa ggctttgatc tggccattct tcgggttgat ctcaatgcga
     1141 agttcccgcg tgccggcggt aaaactcttt ttcgaggcag caagcaacgc ggaggagatg
     1201 gcttccacca gcacctcccg cttgattcct ctttctcgct cgaggtaatc gagcatcgca
     1261 atgagttcag aattcatggg aaaagtattt tagcacgcgg tggcagttac ccgccagcgt
     1321 gaatgcagta ggacatattg acccggctgg agccatgggt caagttgcaa gccaagttta
     1381 tttcagagcc gcggcggcgg ggaaggcaaa ttcgatttcc tcgggacggt aaggggtcgc
     1441 ccgcggtttc gcgagcagaa atccctgcac gtagtccact cccagggaaa cggctgcggc
     1501 cagttcgcgc tcattttcca ctttttcggc cacggtttgg atgcccgcat agtgggagat
     1561 ttcctggatc gaacggagaa tggcctgatt atagggctcg tccgagagat cgtgaatgaa
     1621 gaagccgtcg atcttgagca aatccaccgg caaggtcttg aggtaacgca ggggggaaaa
     1681 tcccgcgcca aaatcgtcca gcgcgaaccg gcagcccata agccgcagcc gcgtcatgat
     1741 ggttcgcgcc tgctcgaagt tcgagatcgg ctccgtctcg gtgacttcca gaatcaggcg
     1801 cgaggcgccg atgccgtggc tcgcgagcaa ttgctcgacc gtgtcgatca ggttgaggtt
     1861 gcacagggag ctcgcgtgga gattggcgga caagatcagg tccggatact cgcgcatggc
     1921 ctcgcagctt ttctgtagca cgaagcgatc cacgtcgtag gacaatcccg ccgcggaaat
     1981 ccgctggatg aaggcggcgg cgcgcgccgg cgcatcggcg cccgggagga acaatcgcag
     2041 aagcgcttcg tggaaaaaga tttgaccggt gatgggtgaa gtgatcgg
//
LOCUS       DATDAY010000350         2856 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_322750, whole genome shotgun sequence.
ACCESSION   DATDAY010000350 DATDAY010000000
VERSION     DATDAY010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2856)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2856)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2856
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_322750"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2530)
                     /locus_tag="VIS74_06990"
     CDS             complement(<1..2530)
                     /locus_tag="VIS74_06990"
                     /inference="COORDINATES: protein motif:HMM:NF014026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HEY5793025.1"
                     /translation="MKRFLPAIRPRPLRISRRLRAVILCALLPAATAAAQAPDPAMEQ
                     AKAILAKMTLEEKVSLCHGASTMSIGAVPRVGIADEFVMSDGPHNVREDLVRDTFQSA
                     GRKDDTSTSLPPLTTLAATWDVAMAAKHGHTLAQETRDRGKDMILGPGVNILRTPLNG
                     RNFEYLGEDPHLAATLAVAEIKAIQANDVAACVKHFAANNQELNRHGVSAEIDERTLR
                     EIYFPAFEAAVKEGEVLTVMSGYNRFRGEHCSHSDFLNNQVLKKQWGFKGFVVTDWGG
                     LGSTVQGALGGTDVEMNAGRNIRFFNQPLLDAVKDGKLPESVVDDKALRVLYVMARLR
                     KLDGQPRAKGSRNTPENQQASREIAEAGIVLLKNGGNVLPLDAKALKSVLVLGKMAIE
                     KKCMKGGSSNGKPPYEVTPLEGLQKRLGPGVEVAYLPLSDNEKGLNLTEIPETAINTF
                     DTEIKDAGLAVRAWRAEYFNTPSPEGSPAAAGFERKLDFNWQKAAPKPGVNADHFSAR
                     WTAKIIAPETGSYVLSVGGDGGARLSVDGKTVLENWSATGKLMAAGTVDLAAGKEYEF
                     QVEYFDERDNAAFSLKWQLPSDRAMDPAQIKAQVAKAGAVVIFTGAGHAFESESFDRQ
                     HMKLPDGQDAAVAALLGINPKTVIVNLGGTPVEMPWIKQAPAVVQYWYSGQEGGHALA
                     AILFGDVNPSGKLPFTFPVKLADSPAHALGNYNGKSVNHAEGIFVGYRWFDAKKIEPL
                     FPFGYGLSYTAFDYKNVKLSSPSLEPNGQVQVSVDVTNTGKRAGAEVVQLYVRDPKPP
                     IEKAVRELKGFAKVALQAGETKTVTLTLKARDFAWFDVPGMQWKA"
     gene            complement(2556..>2856)
                     /locus_tag="VIS74_06995"
     CDS             complement(2556..>2856)
                     /locus_tag="VIS74_06995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5793026.1"
                     /translation="SGKVDEAGKSRAYNGAVIPIGQVRFDIRFDPKPLSVSEGDEVGL
                     QLLFCPFGWQWSASARPQMKMSAQAALLEVRIPALAKLAPRVNYVWNGKSLIPKE"
BASE COUNT          445 a          895 c          950 g          566 t
ORIGIN      
        1 ccgctttcca ttgcatgccc ggcacgtcga accaggcaaa atcgcgcgcc ttcagcgtga
       61 gggtgacggt tttggtctca ccggcttgca gcgcgacctt ggcaaaaccc ttcaactcgc
      121 gaaccgcttt ctcgatcggc ggcttgggat cgcgcacgta gagttgcacc acttcggcgc
      181 cggcgcgctt gccggtattg gtgacgtcca cgctcacttg cacctggccg ttcggctcca
      241 ggctggggct ggagagcttg acgtttttgt agtcaaatgc ggtatagctg agtccgtaac
      301 cgaaggggaa cagcggctcg attttcttcg cgtcgaacca ccggtagccg acgaaaatgc
      361 cttcggcgtg gttcacgctc ttcccgttgt agttgcccag cgcgtgggcc gggctgtcgg
      421 ccagcttcac cgggaaggtg aacggcagct tgccggaggg attcacgtcg ccaaagagga
      481 tcgccgccag cgcgtggccg ccctcctggc cggaatacca atactgcacc accgcgggcg
      541 cctgcttgat ccagggcatt tccacgggcg tgccgccgag gttgacgatc acggttttgg
      601 gattgatgcc gagcaacgcg gcgacggcgg cgtcctgccc gtcgggcagc ttcatgtgct
      661 ggcggtcgaa gctctccgac tcgaaggcgt gccccgcgcc ggtaaagatg acgaccgcgc
      721 cggccttcgc gacctgggcc ttgatttgcg cgggatccat cgcgcgatcc gagggcagct
      781 gccacttgag actgaaggcc gcgttgtcgc gctcgtcgaa atattccacc tggaattcat
      841 attccttgcc cgccgcgaga tcgaccgtgc cggccgccat gagcttgccg gtggcggacc
      901 aattctcaag gacggtcttg ccatccacgg agaggcgcgc gccgccgtcg ccgcccacgc
      961 tcaggacgta ggagcccgtc tcgggagcga tgattttggc ggtccagcgg gcggagaagt
     1021 ggtccgcgtt cacgccgggt ttcggcgcgg ccttctgcca gttgaagtcg agcttgcgtt
     1081 caaatcccgc cgcggccggg ctgccttccg gtgaaggggt gttgaaatac tccgcgcgcc
     1141 aggcccgcac ggccaggccg gcgtccttga tttccgtgtc gaacgtgttg atggcggtct
     1201 cgggaatctc ggtgagattg agccccttct cgttgtcgga aagcggcagg taggcgacct
     1261 cgacgcccgg cccgaggcgc ttttgcaggc cctcgagcgg cgtgacctca tagggcggct
     1321 tgccgttgga gctgccgccc ttcatgcact ttttctcgat cgccatcttg ccgagcacga
     1381 gcacgctttt gagcgccttc gcgtccagcg ggagcacgtt tccgccgttt ttcaggagca
     1441 cgatgccggc ttcggcgatc tcgcgcgacg cctgctggtt ctccggcgta ttgcgcgagc
     1501 ccttcgcgcg cggctggccg tcgagcttgc gcagccgggc catgacgtag agcacgcgca
     1561 gggccttgtc gtccacgacc gactccggca gcttgccgtc cttcacggcg tcgagcagcg
     1621 gctggttgaa aaagcggatg ttccggccgg cgttcatctc cacgtccgtg ccgcccagcg
     1681 ccccctggac ggtgctgccc aggccgcccc agtcggtgac gacgaagccc ttgaagcccc
     1741 attgtttctt gaggacctgg ttgttgagga aatcgctgtg gctgcaatgc tcgccgcgga
     1801 agcggttgta gccgctcatt acggtgagca cctccccttc cttcacggcg gcctcgaaag
     1861 ccggaaaata gatttcgcgc agcgtgcgct cgtcgatctc ggcgctgacg ccgtggcggt
     1921 ttaattcctg gttgttggcc gcaaagtgct tcacgcaggc ggccacgtcg ttggcctgga
     1981 tggccttgat ctcggcaacg gccaaggtgg cggcgagatg gggatcctcg cccagatatt
     2041 cgaaattgcg gccattgagc ggggtgcgca ggatgttgac gccgggaccg aggatcatgt
     2101 ccttgccgcg gtcgcgcgtt tcctgcgcca gcgtgtggcc gtgcttggcg gccatcgcca
     2161 cgtcccaggt ggcggcgagg gtcgtgagcg gggggagcga ggtggacgtg tcgtccttgc
     2221 gtccggcgga ttggaacgtg tcgcgcacca gatcctcacg cacgttatgc ggcccgtcgg
     2281 acattacgaa ctcgtccgcg atgcccacgc ggggaacggc gccgatggac atcgtgctcg
     2341 cgccgtggca gagcgagact ttttcctcga gcgtcatctt cgcgaggatg gccttggcct
     2401 gctccatggc ggggtcgggt gcctgggctg ccgcggtggc ggctggaagg agggcgcaca
     2461 gaataacggc tcgcaggcgc cggcttattc tgagtggccg ggggcggatc gcgggaagga
     2521 atcgtttcat aaaagggtca aaaatcggcg gcggctcatt ctttgggaat cagggatttc
     2581 ccgttccaga cgtagttcac ccgcggcgcg agcttcgcca aggcgggtat tcggacttcg
     2641 aggagcgcgg cctgggcgga catcttcatt tgcgggcggg ccgaagcgga ccattgccag
     2701 ccaaacgggc aaaacaaaag ctgcaggccg acctcgtcgc cctcgctaac ggaaagcggc
     2761 ttgggatcga accggatgtc aaagcgcacc tgcccgatcg gaatcaccgc tccgttatac
     2821 gcccggcttt ttcccgcttc atccaccttg ccggac
//
LOCUS       DATDAY010000351         2391 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_661463, whole genome shotgun sequence.
ACCESSION   DATDAY010000351 DATDAY010000000
VERSION     DATDAY010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2391)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2391)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2391
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_661463"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(120..347)
                     /locus_tag="VIS74_07000"
     CDS             complement(120..347)
                     /locus_tag="VIS74_07000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HEY5793027.1"
                     /translation="MRTTLTLDDDVLESARALAAQRGVPIGTVISDLARRGLALVPFG
                     KVRNGIRLFSMYSDADSLTPEIVKALAEEVD"
     gene            541..1149
                     /locus_tag="VIS74_07005"
     CDS             541..1149
                     /locus_tag="VIS74_07005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5793028.1"
                     /translation="MNRNLISRVILPAAVLGFATLSSLQAQSAPPAGGPPEEAPPTAG
                     ELAAYLAKLDAVPGYGGIAFGGEIAKSKDSLVLEQDRGPLKIYKKKGETLLLGPALLE
                     TVLYYFYEGKLYGVAFHTNDGQDSVNLKSILHSAFGPGVNSAEGGPSTIWLGKKVGAL
                     FELNTSTGDASAFLFDQAAHDAYLKYESDAGQKAAELLLKGK"
     gene            1154..1456
                     /gene="grrA"
                     /locus_tag="VIS74_07010"
     CDS             1154..1456
                     /gene="grrA"
                     /locus_tag="VIS74_07010"
                     /inference="COORDINATES: protein motif:HMM:TIGR04260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrrA/OscA1 family cyclophane-containing
                     rSAM-modified RiPP"
                     /protein_id="HEY5793029.1"
                     /translation="MKLTSHLKALLLGGTVALTASQTASAAGKAPAPKSESLDARIEK
                     IRQQAGQNPADSNFRTGSGPQSDADLMWWRNAWGNGGWHNWHNGWRNGGWGNWHNW"
     gene            1483..>2391
                     /gene="grrM"
                     /locus_tag="VIS74_07015"
     CDS             1483..>2391
                     /gene="grrM"
                     /locus_tag="VIS74_07015"
                     /inference="COORDINATES: protein motif:HMM:TIGR04261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclophane-forming radical SAM/SPASM peptide
                     maturase GrrM/OscB"
                     /protein_id="HEY5793030.1"
                     /translation="MSSVPLLDPASAPPEPGSLSDSSWLKLVVLQPSPFCNINCDYCY
                     LTRRGDTHRMSRETLQRAVEEIFASDLIAEQVTFVWHAGEPLAVPIPWYEEAFDLIAR
                     HAPPQVRIGHSFQSNGTLLNEKWCRFIRDRGLSIGLSLDGPAFIHDAHRVTRQGAGTH
                     ALAMRGANQLREHGIFFNVIAVVTRESLDHPDAIFDFFLENGMHHVGFNIEELEGGNT
                     QSSLQDVREDRIAAFFRRIFERQKAEKGKVRIREFDSALARILGGSGNAAEFVYENEQ
                     VRPFGILSVDWQGNFSTYSPELLGLES"
BASE COUNT          492 a          793 c          675 g          431 t
ORIGIN      
        1 tcgaaaaccg catccgttcg ctcctaaagg attcggggaa aaatttccaa tgcgaagatg
       61 agtccgccga aatgtcccct tcgccattca ggcgggaatg atctcaaggc aatggcgatt
      121 caatccactt cctccgccaa ggctttgacg atctcaggag tcaacgagtc ggcatcggaa
      181 tacatcgaaa aaaggcgaat tccattgcgc actttgccga atggaaccag ggcaaggccg
      241 cgccgcgcca aatcggaaat aacggttccg attggcactc cgcgttgagc ggccaaggct
      301 cgggccgatt ccaacacgtc atcatccaag gtgagcgtgg ttctcatgca tcaaacatta
      361 cgcatcacac atcgcatgac aacggatgaa tcgccatcga ggtaaagcca tgggtttggc
      421 gtgaaacgct gccggcaaaa accggcgcgg tgaccaaaac gtaacctaaa ttcgctctgg
      481 cgcgcggcgg cgctttcggt aatatgcccc ccagcgaacg gagttcaccc ctaagttttt
      541 atgaatcgca acctcatctc acgagtcatt ctcccggcgg ccgtgctggg attcgccacc
      601 ctctcctccc tgcaagccca gtccgcgccc cccgcgggcg ggccgcccga agaggctccg
      661 cccacggccg gggaactggc cgcctatctc gcgaaattgg acgcggtccc cggctacggc
      721 ggcatcgcct tcggcgggga aatcgcgaag tccaaggatt ccctggtgct ggagcaggac
      781 cggggtccgc tgaagatcta caaaaagaaa ggcgaaaccc tcctgctcgg ccccgcgctg
      841 ctggaaacgg tgctgtatta tttctatgaa ggcaaacttt acggggtggc gttccacacg
      901 aatgacgggc aggacagcgt caatctgaag agcatccttc actccgcgtt cggacccggt
      961 gtcaattccg ccgagggggg gccgtccacc atctggctgg gcaaaaaagt gggcgcgctc
     1021 ttcgagctca ataccagcac cggcgacgcc agcgcctttc tcttcgacca ggccgcgcac
     1081 gatgcctacc tcaaatacga atccgacgcc ggccagaagg ccgccgaact gctcttgaaa
     1141 ggaaaataaa cccatgaaac tgacctcgca cctcaaagcc ctgcttctcg ggggaaccgt
     1201 cgcgctcacc gcttcgcaaa ccgcctccgc ggcggggaaa gcgccggcgc cgaaatccga
     1261 atccctcgat gcccgcatcg aaaagatccg ccagcaagcc ggccagaatc ccgccgattc
     1321 gaatttccgc accggctccg gcccgcagag cgacgccgat ctgatgtggt ggcgcaatgc
     1381 ctggggcaat ggcggctggc ataattggca caacggctgg cgcaacggcg gctggggcaa
     1441 ctggcataac tggtaagccg cccaccgacg gaccggccct tcatgtcttc cgtccctttg
     1501 ctggacccgg cttccgcccc accggagccg ggttcgcttt ctgattcttc ctggctgaag
     1561 ctcgtcgtgc ttcagccttc gccgttttgc aatatcaact gcgattattg ctacctcacc
     1621 cgccgcggcg acacgcaccg catgagccgc gaaaccctcc agcgcgccgt ggaggagatc
     1681 ttcgcctcgg acctcatcgc ggaacaggtc accttcgtct ggcacgccgg ggaacccctc
     1741 gccgtcccga tcccctggta tgaggaggcc ttcgacctca ttgcccggca cgcgccgccg
     1801 caggtccgca tcggccattc ctttcagagc aatggcacgc tcctcaacga aaaatggtgc
     1861 cgcttcatcc gcgatcgggg tttaagcatc gggctgagcc tggacggccc ggcgttcatt
     1921 cacgacgccc accgcgtcac ccgccagggc gccggcaccc atgccctggc catgcgcggc
     1981 gcgaaccagc tgcgcgagca cggcatcttt ttcaacgtca tcgccgtcgt cacgcgggag
     2041 tcgctcgatc atcccgacgc gatcttcgat tttttcctcg aaaacggcat gcaccacgtc
     2101 ggtttcaata tcgaggagct ggagggcggc aacacacaga gctcgcttca ggatgtgcgc
     2161 gaggaccgca tcgccgcctt tttccgccgg atcttcgaac ggcagaaggc cgaaaagggc
     2221 aaggtgcgca tccgcgagtt cgactccgcg cttgcccgga ttctcggcgg cagcgggaat
     2281 gcggcggagt tcgtttacga aaacgagcag gtccgcccct tcggcatcct gagcgtggac
     2341 tggcagggaa acttttcgac ctactcaccc gagctgctcg gcctggaaag c
//
LOCUS       DATDAY010000352         2203 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_2253299, whole genome shotgun sequence.
ACCESSION   DATDAY010000352 DATDAY010000000
VERSION     DATDAY010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2203)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2203)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2203
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2253299"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..192
                     /locus_tag="VIS74_07020"
     CDS             <1..192
                     /locus_tag="VIS74_07020"
                     /inference="COORDINATES: protein motif:HMM:NF017045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5793031.1"
                     /translation="AARLGWKIGNLHFSVEVTADALRVADDSAIRQMLGREGIAHAAR
                     SAVFRPLLAAPHSHSHAHD"
     gene            185..877
                     /locus_tag="VIS74_07025"
     CDS             185..877
                     /locus_tag="VIS74_07025"
                     /inference="COORDINATES: protein motif:HMM:NF013858.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory UreF family protein"
                     /protein_id="HEY5793032.1"
                     /translation="MIESGEAGLAFLLQTSDAMFPTGAYAHSLGMEEMIRAGHVFDEA
                     SLLAFLRRHILPAARRVDLPLAAWAHRAARQNDFAALLETDGLAGALRPARELRAASL
                     QTGRRRLAMLAAVRPAPLLREYQRAAEESPHVGHHAPVWGAACAAVSLPAALTAYHYQ
                     TLACFCAAAPKLLRIGQEAAQRVLTAALAEAGAAIEFAQRVERGDIGWFDPVLDIASM
                     RHEIADERLFIS"
     gene            874..1476
                     /gene="ureG"
                     /locus_tag="VIS74_07030"
     CDS             874..1476
                     /gene="ureG"
                     /locus_tag="VIS74_07030"
                     /inference="COORDINATES: protein motif:HMM:TIGR00101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreG"
                     /protein_id="HEY5793033.1"
                     /translation="MKTLRIGIGGPVGSGKTMLVLRLCEWLRGEFDMAVVTNDIYTRE
                     DAEFLVRNQALPAERVRGVETGGCPHTAIRDDTSMNDAAVAELRQSFPELQLILIESG
                     GDNLSATFSPELVDVFIYVIDVAQGDKIPRKGGPAIRRSDLLLINKISLAPHVGADLD
                     VMARDARLMRGDRPFLFADLKSQQGREDLLAWLRREYLFV"
     gene            1483..>2203
                     /locus_tag="VIS74_07035"
     CDS             1483..>2203
                     /locus_tag="VIS74_07035"
                     /inference="COORDINATES: protein motif:HMM:NF013899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreD"
                     /protein_id="HEY5793034.1"
                     /translation="MTGHLHIELAADAAGRTFIRGQSFHAPMHLSKPHWDGNCLVVQA
                     VNCTAGLFGGDRIDFSVHAGAGTRALVTSPSAQRAYPARTADSVAWIEQRFTVEPGAW
                     LETFPEIFIPHRGARVRQNTRIDAQSGAELFYLESLAPGRVGAGEAFAYERLEWLTEL
                     FLGGKSLVRERYALEAGGDSVELLRSHFPDAYYASGYLVSPKLPGVERFRQEIAGLGD
                     AETRIAASFPVPEVAVIKILAG"
BASE COUNT          326 a          738 c          769 g          370 t
ORIGIN      
        1 gccgcccggc tcggctggaa gatcggcaat ctgcacttct cggtcgaggt caccgcggac
       61 gccctccgcg tggccgacga ttccgccatc cggcaaatgc tcggccggga ggggatcgcc
      121 cacgccgccc gcagcgccgt ctttcgcccg ctgctggccg cgccgcacag ccattcgcat
      181 gcccatgatt gaatccgggg aagcggggct ggcctttctg ctgcaaacct cggacgccat
      241 gtttcccacc ggcgcctatg cgcattcgct gggcatggag gaaatgatcc gcgccggcca
      301 cgtgttcgac gaagccagcc tgctggcgtt tttgcgccgg cacattctgc cggccgcccg
      361 gcgcgtggat ctgccgctcg ccgcctgggc gcaccgcgcg gcgcggcaaa acgatttcgc
      421 cgcgctgctg gaaaccgacg gtctcgccgg ggcgctgcgc ccggcgcggg aactgcgcgc
      481 ggcgagcctg caaaccgggc ggcggcggct ggccatgctg gccgcggtgc gcccggcgcc
      541 gctgctgcgg gaataccaaa gggccgcgga ggaatccccc catgtcgggc accatgcccc
      601 ggtgtggggc gcggcctgcg cggcggtttc gctcccggcg gcgctgacgg cgtatcatta
      661 ccaaaccctc gcctgcttct gcgccgcggc gcccaagctg ctgcgcatcg gccaggaggc
      721 ggcccagcgg gtgctgacgg cggcgctcgc ggaggccggg gcggccattg aattcgccca
      781 gcgggtcgaa cgcggggaca tcggctggtt cgacccggtg ctcgacatcg cctccatgcg
      841 gcacgagatt gccgacgaac ggctgtttat ttcatgaaaa ccctgcgcat cggcatcggc
      901 gggccggttg gctccggaaa gaccatgctc gtcctgcggc tctgcgagtg gctccgcggc
      961 gagttcgaca tggcggtggt caccaatgac atctacaccc gcgaggacgc ggaatttctc
     1021 gtccgcaatc aggcgctgcc ggcggaacgg gtgcgcgggg tggaaaccgg cgggtgcccg
     1081 cacacggcga ttcgcgacga cacgagcatg aacgacgccg cggtggcgga actgcggcag
     1141 tcctttccgg agctgcagct catcctcatc gaaagcggcg gcgacaacct ttccgccacg
     1201 ttttcaccgg agctggtgga tgtgttcatt tacgtgatcg atgtcgcgca gggggacaaa
     1261 atcccgcgca agggcgggcc ggccattcgc cggagcgatc tgctgctcat caataaaatc
     1321 tcgctggcgc cccacgtcgg cgcggatctg gacgtgatgg cgcgcgacgc ccggctgatg
     1381 cgcggcgacc ggccgtttct gttcgccgac ctcaaatccc agcagggccg cgaggacctg
     1441 ctcgcgtggc tgcggcgcga gtatttgttt gtctaagcgg cagtgacggg ccacctccac
     1501 attgaacttg cggcggacgc ggccgggcgg acgttcattc gcgggcagtc cttccacgcg
     1561 ccgatgcacc tgagcaagcc acactgggac gggaactgcc tcgtcgttca ggcggtgaat
     1621 tgcaccgcgg gtttgttcgg cggcgaccgg attgattttt cggttcacgc cggggcgggc
     1681 acgcgcgcgc tcgtcaccag cccgagcgcg cagcgggcgt atccggcgcg gacggccgat
     1741 tcggtggcgt ggatcgagca gcggtttacc gtcgagcccg gcgcctggct ggaaacgttc
     1801 ccggagattt tcattccgca ccgcggcgcg cgcgtccggc aaaatacgcg gatcgacgcg
     1861 caatccggcg cggagttgtt ttatctcgaa tccctcgctc ccggccgggt gggcgccggc
     1921 gaggcgttcg cctacgaacg gctggaatgg ctgaccgaac ttttcctcgg cggaaaaagc
     1981 ctcgtgcggg agcgttacgc cctggaggcg ggcggcgaca gcgtggagct gctgcggagc
     2041 cattttccgg acgcttatta cgcctccggt tacctcgtct ccccgaagct ccccggagtg
     2101 gagcggttcc ggcaggaaat cgccgggctg ggcgacgcgg agacgcgcat cgccgccagt
     2161 tttcccgtgc cggaagtggc ggtgatcaag atccttgccg gcg
//
LOCUS       DATDAY010000353         2974 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_1159882, whole genome shotgun sequence.
ACCESSION   DATDAY010000353 DATDAY010000000
VERSION     DATDAY010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2974)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2974)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2974
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1159882"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            128..1159
                     /locus_tag="VIS74_07040"
     CDS             128..1159
                     /locus_tag="VIS74_07040"
                     /inference="COORDINATES: protein motif:HMM:NF013219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEY5793035.1"
                     /translation="MTRNRLKWICLAAMAISAPLVFTLALSIGAVRVPVDEIAAILLG
                     QANDSLKEAVIWNIRLPRVLLGLLTGAALAVSGAALQGLFRNPLADPGIIGIANGAAL
                     AAAATIVFGSLVFHLIPRNLIPLALPAGAFGGGLITTMLIYRLSWHEGRTVAATLLLA
                     GIAISALTAAATGLFTYLATDEQMRTLVFWTLGSLGGASWTSCLLAAICVVPALFFLL
                     KESRALNAMLLGEAEAGFLGFNPEQTKTRVVIASALAVGGSVAFCGVIGFVGLVVPHL
                     VRLLAGADHRFLIPGSALLGASLLTAADIGARTLAAPAELPIGILTALIGAPLFLWLL
                     LKQRAAFAG"
     gene            1268..2023
                     /locus_tag="VIS74_07045"
     CDS             1268..2023
                     /locus_tag="VIS74_07045"
                     /inference="COORDINATES: protein motif:HMM:NF010068.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter ATP-binding protein"
                     /protein_id="HEY5793036.1"
                     /translation="MLTGTQLTVVRQHRRLVSEVDFTARSGEVTAILGRNGAGKSTLL
                     RCLSGELKPAAGTVAYFDRALPDWKPLELARIRAVVAQSSSLSFPFRLREVVELGRSP
                     HSTQSDGPVQWALAQLDLLSLADRDYPTLSGGERQRTHFARALAQIYEAAETGRGFLL
                     LDEPTSSLDLAHQHQALRCAKALTGRGLGVVAVLHDLNLCARYADTVYLLDEGIRLAC
                     GPAAEVLDPPLLSRAYRIPIRKAGGAEMFDVEN"
     gene            complement(2081..2644)
                     /gene="nusG"
                     /locus_tag="VIS74_07050"
     CDS             complement(2081..2644)
                     /gene="nusG"
                     /locus_tag="VIS74_07050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEY5793037.1"
                     /translation="MALAPRDQWYVVHVLSGQESKVQENILKRIKTEEVGDVIFDVLI
                     PTERVQEIKRGKKTETTRKFFPGYILINMHLLDDNNQLVERAWYFIRETNGVIGFAGS
                     KDKPTPMRPKEVEGMLAQVAEREDSVKPKISFEVGDKVKVADGPFQNQNGVVEEIDPE
                     RGKLRVSVSIFGRDTPVELEYWQVERA"
     gene            complement(2652..>2974)
                     /locus_tag="VIS74_07055"
     CDS             complement(2652..>2974)
                     /locus_tag="VIS74_07055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HEY5793038.1"
                     /translation="GLQNRRLGVRVPPGLLSECSMAPRHSRQNSHFTVLMFRKTGKFV
                     GEVRAELGKAQWPWDPNEKGFKRYKELWDSTLVVLIAMLLLGGYIAFFDVWLVNIVRF
                     LTQL"
BASE COUNT          517 a          898 c          947 g          612 t
ORIGIN      
        1 cgtcgaactg gcggcggcca tccggccgtg aatgccgtgc gaggctgcgc tcttaaccgt
       61 ggcatcggcc tcccgccgat gagccaatta atcgcatcgg ctggaagcca atgccactcc
      121 gcctgaagtg acccgcaacc gtctgaaatg gatctgcctg gcggccatgg cgatttcggc
      181 gcccttggtt ttcacgctgg ctttatccat cggcgcggtg cgggtgccgg tggatgaaat
      241 cgcggccatt ttgctgggtc aggcgaatga ttcgctgaag gaagcggtga tctggaacat
      301 ccggctgccg cgagtgctgc tggggttgct caccggggcg gcgctggccg tcagcggcgc
      361 ggccttgcag gggctctttc gcaatcccct cgcggacccc ggcatcatcg gcatcgccaa
      421 cggcgcggcg ctggcggcgg cggcgaccat tgtttttggc tccctggttt ttcacctgat
      481 cccgcgaaac ctgattcccc tggcgcttcc ggcgggcgca ttcgggggcg gattgatcac
      541 tacgatgctg atctatcgcc tgtcctggca cgaggggcgg accgtcgccg cgacgctcct
      601 gctggcgggc attgccatca gcgcgctgac ggcggcggcc accggtttgt tcacctatct
      661 ggcaacggat gaacaaatgc gcacgctggt tttttggacg ctgggcagtc tgggcggagc
      721 ttcgtggacg agctgcctcc ttgccgcgat ctgcgtcgtt cccgccttgt tttttctgct
      781 gaaggaaagc cgggcgttga acgcgatgct gctcggagaa gcggaggccg gctttctggg
      841 atttaatccc gaacagacca aaacgcgcgt cgtcatcgcc tcggcgctgg ccgtgggcgg
      901 aagcgtggct ttctgcggcg tgatcgggtt tgtcggcctc gtcgttccgc acctcgtccg
      961 gctgctggcc ggagcggatc atcgtttcct gattcccggt tccgcgctgc tgggcgccag
     1021 cttgctgacg gcggcggaca tcggggcccg aaccctggcg gcgccggcgg agctgccaat
     1081 cggcatcctg acggcgctca ttggagcccc ccttttcctt tggctgctgc ttaaacaacg
     1141 cgcggccttt gccggctaga gccgtttcga ccgagatgaa cccaaatttt gcgaagaaag
     1201 ccacggcggt ttcatcgaaa ccgccctacc cgggttcgga tagaaacggc tcgaatgcgc
     1261 gaactccatg ctgaccggaa cgcagctcac cgtcgtccgc cagcaccggc ggttggtgtc
     1321 cgaagtggat ttcaccgcaa ggtccggcga agtcaccgcg attctgggcc ggaatggcgc
     1381 gggcaaaagc acgctgctgc gctgcctgag cggggagttg aagccggccg cgggcacggt
     1441 ggcctatttt gaccgggcgc tgccggactg gaaaccgctg gagttggcgc gaatccgcgc
     1501 ggtggtcgcg caatccagca gcctgtcctt tcccttccgg cttcgcgaag tcgttgagct
     1561 cggacgcagc ccgcactcga cgcaaagtga cgggccggtg caatgggccc tggcgcagct
     1621 ggacctgctg tccctggcgg atcgggatta cccgacgtta tccggcgggg aacgccagcg
     1681 cacccacttt gcccgggcgc tcgcgcagat ttacgaggcg gccgagaccg gccggggatt
     1741 tttgctgctg gacgaaccca cttccagcct ggatctcgcc catcaacatc aggcgctgcg
     1801 ctgcgccaag gctctcaccg ggcggggatt gggagtggtg gcggtcctgc acgaccttaa
     1861 cctttgcgcc cgatacgcgg acacggttta cctgctggac gaaggcatcc gcctcgcgtg
     1921 cggcccagcc gccgaggtgc tcgatccgcc gctcttgagc cgggcatacc ggattccgat
     1981 tcggaaggcg ggcggcgccg agatgttcga cgtggaaaac tgaactaagc ttctgctcat
     2041 agaaattatg ggatctatga gacttataag ctaaaaaaac tcaggccctt tcgacctgcc
     2101 agtattcgag ttcgaccggg gtgtcgcggc cgaagatgct gacggagacg cggagtttgc
     2161 cgcgttcggg atcgatttcc tcgaccacgc cgttctggtt ctggaagggg ccgtcggcca
     2221 ccttgacctt gtcgccgacc tcgaaggaaa tcttgggttt gacgctgtcc tcgcgttccg
     2281 cgacctgggc gagcatgccc tccacttcct tcggacgcat gggagtaggc ttgtccttgg
     2341 agccggcgaa gccgatgact ccgttggttt cacggatgaa ataccaggcg cgctccacga
     2401 gctggttgtt gtcatccagc aggtgcatgt tgatgagaat gtagccgggg aaaaatttgc
     2461 gggtcgtttc ggtttttttg ccccgcttga tctcctggac ccgctcggtg gggatcaata
     2521 cgtcgaaaat gacatcgccc acttcctcgg ttttgatgcg cttgaggatg ttttcctgca
     2581 ccttcgactc ctggccggag agaacatgca cgacatacca ttgatcacga ggagcgagag
     2641 ccattataaa actagagttg ggtcaggaaa cgaacgatat tcaccagcca cacgtcgaag
     2701 aaggcaatgt aaccgcccag cagcagcatg gcgatgagca cgacgagggt ggaatcccac
     2761 aattccttgt agcgcttgaa gcctttttca ttgggatccc agggccactg ggccttgccc
     2821 agctcggcgc gaacctcgcc aacaaatttt ccggttttgc gaaacatgag aacggtgaaa
     2881 tgggagtttt gccgcgaatg gcgcggcgcc attgaacatt ccgacaacag gccaggaggg
     2941 actcgaaccc ccaaccgacg gttttggaga ccgc
//
LOCUS       DATDAY010000354         2932 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_1033985, whole genome shotgun sequence.
ACCESSION   DATDAY010000354 DATDAY010000000
VERSION     DATDAY010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2932)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2932)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2932
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1033985"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1765
                     /locus_tag="VIS74_07060"
     CDS             <1..1765
                     /locus_tag="VIS74_07060"
                     /inference="COORDINATES: protein motif:HMM:NF012618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5793039.1"
                     /translation="AADLRAWLAGQTVVARPVGAGGRLLRWIRRKPVLAGLAGALFAA
                     IASLGIHIALARGAALASEQHRANLAERFAREQQRNALLAGAQLRLRMRDAGRRSESL
                     RWLREAWRLGPSVEIRSAAITALALPDAEEARPPPGMTFAEPAGAPEPGFALPSPVLT
                     SAFHPGSHRLAAAASDKLVYIVDVQRRAIVRRLHGVGGRCRALSFSPDGHWLASVVDD
                     GTLRLWSVRGGEELLVIDRAIFEKNVRLRWSADGQWLVLAPGRAFRIRAPEAARFFIP
                     EAAENRAEEIRTIDLSADGRWLVTGDESGTRLWDARTRREAALFAKDGAEWSAAGFSP
                     DSRRLWIGGWNSALRVADLPAVENAAPAAPSKVAELAGVLIEQSEDGSWLAALSNDAG
                     GFQFLSSAAPRREVWLRHPHPLDLALTRDARRAATSSYDSAGVRIWDFPSARLLRELP
                     AAPPAQLAFSPDGATLATGGKHEVTLWNADTGKRGRTLPTIGLINSLAFSPDGLLLAV
                     ETHAGIIFFRATEPFEELARLATVPDGGSASFRFSRDSRQLAMQTSAGGAVVWQLDAL
                     HRELAALGMAWDQPLPNAPQK"
     gene            complement(1796..>2932)
                     /gene="rpoN"
                     /locus_tag="VIS74_07065"
     CDS             complement(1796..>2932)
                     /gene="rpoN"
                     /locus_tag="VIS74_07065"
                     /inference="COORDINATES: protein motif:HMM:TIGR02395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HEY5793040.1"
                     /translation="GSYSAEAQERRQFFFDSLTRPLSLREALSEQLALIDLLPMERAL
                     STAILGNVDESGYLAARSEEIAAQQRVTPLQVEEVLEKLQERLEPAGIAARDLRECLL
                     LQLRRAGREQTLDYRIVEGHLEQLARRKFQEIARELKVTAANVQQTLENISRLDPRPG
                     HQFSAEPDQIIVADVFVRPSEGGGYEVFLNQQELPHLRISNTYKDMLGMPGADNREIR
                     EYIREKLRGGRFFLRSLEQRNQTILSIAKEIVQRQREFFDHGSSHLTPMTMGQVAEAV
                     GVHETTVSRAVSGKYMESPQGLFELKYFFTAGYRTAEGQAVSNESVRQTIADLVAGEN
                     SCRPLSDQDMVDILEERGIPVARRTIAKYRDQLGILPSHLRKRG"
BASE COUNT          446 a         1047 c          930 g          509 t
ORIGIN      
        1 ggccgcggac ttgcgggcgt ggcttgcggg tcaaacggtc gtcgcgcgcc cggtcggcgc
       61 cggaggccgg ctcctgcgct ggattcgccg caagcccgtg cttgcgggat tggcgggcgc
      121 gctttttgcg gccatcgcaa gcctgggaat ccacatcgcg ctcgcgcgcg gcgcggcgct
      181 ggccagtgag cagcaccgcg ccaatctcgc ggaacgcttc gcccgcgagc agcagcgcaa
      241 cgcgctgctc gcgggcgccc aattgcggct gcgcatgcgc gacgcgggcc gccgcagtga
      301 aagcctgcgc tggctgcgcg aggcgtggcg gctcgggccg tccgtcgaga ttcgcagcgc
      361 ggccatcacc gcgctggcgc tgccggacgc cgaggaggct cgtccgccgc ccggcatgac
      421 gttcgccgag cccgccgggg cgcccgaacc cggcttcgcc ctgccttccc ccgtcctcac
      481 gagcgccttc catcccggct cgcaccggct ggccgcggcg gcaagcgaca agctggttta
      541 catcgtggat gttcagcggc gcgccattgt ccggcggctg cacggcgtgg gaggccgatg
      601 ccgggcgctc agtttcagcc ccgatggcca ctggctggcg tccgttgtcg atgatggcac
      661 gctgcggctg tggagcgtgc gcggcggcga ggaactgctc gtcatcgacc gcgccatttt
      721 cgaaaaaaac gtgcggctcc gctggtccgc cgacggccag tggctggttc tcgcccccgg
      781 ccgcgcgttc cggatccgcg cccccgaagc ggcgcgcttc ttcatcccgg aagccgccga
      841 gaaccgtgcc gaggaaatcc gcaccatcga cttgagcgcc gatgggcgct ggctggtcac
      901 gggggacgag tccggcacgc gcctctggga cgcccgcacc cgccgtgagg cggcgctctt
      961 cgccaaggat ggcgcggaat ggagtgcggc cggcttttcc cccgacagcc gccggctctg
     1021 gatcggtgga tggaattccg ccctgcgcgt ggcggacctg cccgcggtgg agaatgccgc
     1081 accggcggcc ccgtcaaaag tggccgaact cgccggcgtc cttatcgagc aaagcgaaga
     1141 cggctcctgg ctcgccgcgc tgagcaacga tgccggcgga ttccaattcc tctcctccgc
     1201 cgcgccgcgc cgcgaggttt ggctgaggca cccgcatccc cttgacctcg cgctgacccg
     1261 tgacgcccgc cgcgccgcga cgagcagcta cgactccgcg ggggtgcgga tctgggattt
     1321 cccctccgcg cggctgctcc gcgagttgcc cgccgccccg ccggcccaac tcgcattcag
     1381 tcccgatggc gccaccctgg ccaccggcgg caaacatgaa gtgacgcttt ggaatgcgga
     1441 taccggcaaa cgcggccgca cgctgcccac catcggcctg atcaactccc tcgccttcag
     1501 ccccgatggc ctcctcctcg cggtcgagac gcatgccggc attatctttt tccgcgccac
     1561 ggagcccttc gaggaacttg cgcgcctggc caccgttccc gacggcggct ccgcctcatt
     1621 tcgtttcagc cgcgactccc gccagctcgc catgcagacc tccgccggcg gcgccgtcgt
     1681 ctggcagctc gacgcgctgc accgcgaact cgccgcgctc ggcatggcct gggatcagcc
     1741 gctcccgaat gcgccgcaaa agtagaggag agaaaacgga taaccgttcg gttttctacc
     1801 cgcgcttccg cagatggctc ggcaggattc cgagctggtc gcggtatttc gcgatggtcc
     1861 ggcgggcgac gggaatgccg cgttcctcca ggatatccac catgtcctgg tcgctgagcg
     1921 gacggcagga attttcgccg gccaccagat ccgcgatggt ctggcgcacg ctttcattgc
     1981 tgaccgcctg cccttcggcg gtccggtaac ccgcggtgaa gaaatacttc agctcgaaca
     2041 aaccctgcgg actttccatg tattttccgg aaacggcgcg gctgacggtc gtctcgtgaa
     2101 cgcccaccgc ctcggccacc tggcccatgg tcatgggggt gagatgcgag gagccgtgat
     2161 caaaaaactc gcgctgccgc tggacgattt ccttcgcgat gctgaggatc gtctgattgc
     2221 gctgctccag gctgcgcaga aaaaaacgcc ccccccggag cttttcccgg atatactccc
     2281 ggatttcccg gttgtccgct cccggcatgc cgagcatatc cttataagta tttgagatgc
     2341 ggagatgggg cagctcctgc tggttgagaa agacctcgta tccaccgccc tccgacggcc
     2401 ggacaaacac atccgcgacg atgatctggt ccggttcggc cgaaaattgg tggccggggc
     2461 gcggatccag ccggctgata ttttccaggg tctgctgcac attcgcggcg gtcaccttga
     2521 gttcgcgggc gatctcctgg aatttccggc gggccagctg ctcgagatgg ccttcgacaa
     2581 tccggtaatc cagcgtttgc tcccgccccg cccggcgaag ctgcagcagc aagcactccc
     2641 gcaaatcacg cgccgcgatt cccgccggct ccagcctttc ctgaagtttt tccagcactt
     2701 cctccacttg aagcggcgtc actctttgct gggcggcaat ctcttccgaa cgggcggcca
     2761 gatacccgct ctcatccacg ttccccagaa tcgccgtgga gagcgcgcgt tccatcggca
     2821 acaggtcaat caacgccagc tgctcgctca gcgcctcccg cagactcagc gggcgggtca
     2881 gcgaatcaaa aaagaactgc cggcgttcct gggcttccgc ggaataactg cc
//
LOCUS       DATDAY010000355         2066 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_8269, whole genome shotgun sequence.
ACCESSION   DATDAY010000355 DATDAY010000000
VERSION     DATDAY010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2066)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2066
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8269"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1557)
                     /locus_tag="VIS74_07070"
     CDS             complement(<1..1557)
                     /locus_tag="VIS74_07070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5793041.1"
                     /translation="MKNRVHRSVVPVAAALSALLLGTSAQAQVKTSPAAPVPNASFED
                     GLKSWTGTGDATADMASKSAGEKSLLLRRDPVNLPETKAVSDRFAITPGVWELSGAVA
                     YALYSPDASFNVSITIRSFDPAGKELKARRLLALYEKADWKPVKERVEISLGAATAAL
                     EIMFNKTHGEFRIDNLALGYAGASVPMEGGDRKVTFQSNRPGFLFYPGDHLAVQVTVE
                     TPASLSGKQAVLDWEVTDYWKAPVAAKQRITLESQGKSNRGWNLYRSVLELGALALEV
                     GPYYEIQTRLDLGADTPARDTMSFAVLPESVTKDMDPLDTPFGAHTWNATVYEYFPLA
                     ARLGIRRCLVFWDWPNTAPYTPEFDEGSRRSSRLEWPKRFGLAPYGVLYPVIAIEHRE
                     NKQYTDEALRAGVRQSIEKYRKDGLWGFQIGNEPPGWNPEMVKRDVEAYKAVYEEIKK
                     SDPSVVAIGSAIGPSEDFFKAGFQHYQDAYNLHAYSDLGTLRAAMKQYKELFKKYGGE
                     KPIWSTEIGSK"
     gene            complement(1586..>2066)
                     /locus_tag="VIS74_07075"
     CDS             complement(1586..>2066)
                     /locus_tag="VIS74_07075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5793042.1"
                     /translation="DYTKAQGGNNWFYGYYEGDGPYKPSEFKEMIQKETMWGYEWRAD
                     GIQFLKQGPNLMHPDSRNKAPVVAVRRWKSPVSGKVRIVVDWETWEKGDGIVGKILLD
                     GKEMDSRVVAGPGPGRKSRAEVRTEVRPGSIVEILCHPNQNTQFDATTFQAMIIQEK"
BASE COUNT          363 a          613 c          631 g          459 t
ORIGIN      
        1 cttcgagccg atttccgtgg accagatcgg cttttcacct ccgtattttt tgaagagctc
       61 cttgtattgc ttcatggccg cccgcagcgt gccgaggtcg gagtaggcgt ggaggttata
      121 ggcatcctgg taatgctgga agccggcctt gaagaaatcc tcgctcggcc cgatggcgga
      181 gccgatggcg accacggacg gatcgctctt tttgatctct tcgtagaccg ctttgtaggc
      241 ctcgacatca cgcttcacca tttcgggatt ccagccgggg ggttcgttgc cgatttggaa
      301 gccccagagg ccgtcctttc tgtatttctc gatgctctgc cgcacgcccg cacggagggc
      361 ctcgtcagtg tattgcttgt tctcgcggtg ctcgatggcg atcacgggat acagcacgcc
      421 gtagggcgcc aggccgaagc gcttgggcca ctcgaggcgg ctgctgcggc gtgaaccctc
      481 gtcgaactcc ggcgtgtagg gggcggtatt cggccagtcc cagaacacca gacagcgccg
      541 gatgccgagg cgggctgcca gcgggaaata ttcatacacg gtggcattcc acgtgtgggc
      601 cccgaacggc gtgtccaggg gatccatgtc ttttgtcact gattccggca ggacggcgaa
      661 agacatggtg tcacgggcag gggtatccgc gccgagatcg agccgcgttt ggatttcgta
      721 atacgggccg acttcgagcg cgagggcgcc aagttcgagg acgctgcggt agaggttcca
      781 accccggttg ctcttgcctt gagattcgag tgtgatcctc tgcttcgccg cgaccggtgc
      841 tttccaatag tcggtaacct cccaatcgag aactgcctgt tttccagaaa gacttgcggg
      901 ggtctccacc gtgacctgta ccgcaaggtg gtcgccggga tagaagagaa agcctgggcg
      961 gttgctctgg aaggtcacct tgcggtcgcc gccttccatc ggaacagagg ctccggcgta
     1021 gcccagcgca aggttgtcaa tgcggaactc gccatgggtc ttgttgaaca tgatctcaag
     1081 ggcggcggtg gccgccccca gggagatttc cacgcgttcc ttgaccggct tccaatcggc
     1141 cttttcgtac agggccagca gtcggcgggc tttcaattcc ttgccagcgg ggtcgaagct
     1201 gcgaatggtg attgaaacgt tgaacgacgc atccgggctg tagagtgcgt aggcgaccgc
     1261 accggagagt tcccacactc ccggcgtgat cgcaaagcgg tcgctcaccg ccttggtctc
     1321 cggaagattt acgggatccc gacgcagcag cagggacttc tcgccggctg acttggatgc
     1381 catatccgcc gtggcgtcgc cggtgccggt ccaggatttc aggccatcct cgaaagaggc
     1441 gttcggaacc ggcgcggcgg gtgaggtctt gacttgggcc tgggcggaag ttccaagcaa
     1501 cagggccgac aaggcggcgg cgacagggac aacagaacga tgaacgcgat ttttcatctc
     1561 aagattttcc gggaatccgt ttcccctatt tctcctgaat gatcatcgct tgaaacgtcg
     1621 tggcatcgaa ctgcgtgttc tgattggggt ggcagagaat ttccacgatg gaacccggcc
     1681 gcacttccgt ccggacttcc gcgcgggact tgcgcccggg ccctggcccc gcgacgacgc
     1741 gtgagtccat ttcctttcca tcgagcaaga tctttccgac aatgccgtca cctttttccc
     1801 aagtttccca atcaaccacg atccggactt tgcccgagac cgggctcttc cagcgccgga
     1861 ccgcgaccac cggagctttg ttccggctgt cgggatgcat gaggttcggt ccctgcttta
     1921 ggaactggat gccatcggcg cgccactcgt atccccacat cgtttccttc tggatcatct
     1981 ccttgaactc cgagggcttg tagggtccgt cgccttcata atacccgtag aaccaattgt
     2041 tgcctccttg ggcctttgtg tagtcg
//
LOCUS       DATDAY010000356         2623 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_840799, whole genome shotgun sequence.
ACCESSION   DATDAY010000356 DATDAY010000000
VERSION     DATDAY010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2623)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2623)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2623
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_840799"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..183)
                     /locus_tag="VIS74_07080"
     CDS             complement(<1..183)
                     /locus_tag="VIS74_07080"
                     /inference="COORDINATES: protein motif:HMM:NF014884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5793043.1"
                     /translation="MHIPQLIEFKREGGELSRGQIGEIIRSFTAGELPDYQMSALAMA
                     IFFRGMTPAETAALTAA"
     gene            259..561
                     /locus_tag="VIS74_07085"
     CDS             259..561
                     /locus_tag="VIS74_07085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5793044.1"
                     /translation="MKDKLEQERTDAWTGDAVLSLFARQWILRRYGRMDGDLLGLFTS
                     NQFLLALGNPTSVEAKIGRIFEAEGMAVANAWIERELVPLWEKRLSKKAAAREPGR"
     gene            complement(637..1794)
                     /locus_tag="VIS74_07090"
     CDS             complement(637..1794)
                     /locus_tag="VIS74_07090"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: protein motif:HMM:NF003766.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HEY5793045.1"
                     /translation="MPNFFYPPKHRAILLVLDSAGCGNAPDAANYGDAGADTLGHLLA
                     AFPNLAVPALKQLGLETILSLPGAGQPAASFGTMTECSAGKDTTTGHWEIAGVILTEP
                     FSVFWEFPLDLVDAIEREAGVRFIGNVPASGTEIIERLGDEHLRTGRPILYTSADSVL
                     QIAAHEEIVPLEELYAICQIARKHADPHRIGRVIARPFCGPSGGFRRTANRHDYSFFP
                     PPTILNALEDAGIPVTGVGKISDLFAGSGVSGSHPTGSNREGMEKTGQLWKEMEGGLL
                     FVNLVDFDTLYGHRRDAAGYARALEEFDAWLGGFLPQIQEGDLFILTADHGNDPTHPG
                     SDHTRERVPLCVVDGRPSRDLGIRETYADVAASLAEYFGLPAWPAGRSFLA"
     gene            complement(1848..2186)
                     /locus_tag="VIS74_07095"
     CDS             complement(1848..2186)
                     /locus_tag="VIS74_07095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019517557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HEY5793046.1"
                     /translation="MKKIEAIIKPFKLEEVKEAIADLGVEGMTVSEVKGFGRQKGHTE
                     IYRGSEYTVDFLPKIKIEVVVTDELMEPVVTAIGKAAKTGKIGDGKIFVGPVDQAVRI
                     RTGEEGPSAV"
     gene            complement(2242..2400)
                     /locus_tag="VIS74_07100"
     CDS             complement(2242..2400)
                     /locus_tag="VIS74_07100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5793047.1"
                     /translation="MGTPFKTAEEFIGATGAFHHLANSPVVAGISIGFVALIVVAVIW
                     KSYTIKHD"
     gene            complement(2424..>2623)
                     /locus_tag="VIS74_07105"
     CDS             complement(2424..>2623)
                     /locus_tag="VIS74_07105"
                     /inference="COORDINATES: protein motif:HMM:NF013104.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5793048.1"
                     /translation="PISFMGQLIPVLICIPLGFIPGYVVSLILKKLKMLRVSAEVETD
                     GLDISEIGIDAYPESHLATKH"
BASE COUNT          492 a          778 c          840 g          513 t
ORIGIN      
        1 ggcggcggtg agcgcggcgg tttcggcggg cgtcatgccg cggaaaaaaa tcgccatggc
       61 cagggcgctc atttgatagt ccggcaactc gcccgccgtg aacgaccgga tgatctcccc
      121 gatctgcccg cggctgagct cgccgccctc gcgtttgaat tcgattaact gcggaatgtg
      181 catgaatcgc tgattttgga atggccggac cgcccctttc taatcgaaaa tcggaaatcg
      241 agaatcgaaa atgcggcgat gaaggacaaa ctggagcaag aacgaaccga tgcctggacc
      301 ggcgacgcgg tgctgtcgct gttcgcgcgg caatggattc tgcggcgcta tggccggatg
      361 gatggcgacc tgctcggcct tttcacctcg aatcaatttc tgctcgcgct cggcaatccc
      421 acctccgtcg aggcgaagat cggccgcatt tttgaagcgg agggaatggc ggtcgccaat
      481 gcctggatcg agcgcgagct ggttccgctt tgggaaaaac ggctaagcaa gaaagccgcg
      541 gcacgcgagc cgggccggta ggccgaaccc ctccggcggg ccatatttca aaatagtttc
      601 acaaacgcgc tttggccgcc ggagccgcgg cggtatctaa gccagaaagc tgcggcccgc
      661 gggccaggcc ggcaggccga aatactccgc gaggctggcg gcgacatccg cgtaggtttc
      721 gcgaatgccg agatcccggg agggacgtcc gtccaccacg caaagcggca cccgctcgcg
      781 cgtgtgatcg ctgccgggat gcgtgggatc gttgccgtgg tccgcggtga ggatgaacag
      841 atccccctcc tgaatctggg gcaggaatcc gcccagccag gcgtcgaatt cctccagcgc
      901 ccgcgcatac cccgccgcat cccggcggtg gccgtagagc gtgtcgaaat ccacgagatt
      961 cacgaacagc agcccgcctt ccatttcctt ccaaagctgt ccggtttttt ccatgccctc
     1021 gcggttcgaa ccggtggggt gcgatccgct cacgccgctg cccgcgaaga ggtcgctgat
     1081 cttgccgaca ccggtcacgg ggatgcccgc atcctccagc gcgttcagaa tcgtcggcgg
     1141 ggggaaaaat gaataatcat gccggttcgc cgtccgccga aaaccgcccg aagggccgca
     1201 aaacggccgg gcaataacgc ggccgatgcg gtggggatcg gcgtgcttgc gcgcgatttg
     1261 gcagatggcg tagagctctt cgagggggac gatttcttca tgggcggcga tttgcagcac
     1321 ggaatcggcc gaagtgtaaa gaatggggcg gccggtgcgc agatgttcgt ccccgagccg
     1381 ttcgatgatt tccgtgccgc tggccggaac attgccgatg aatcgcacgc ccgcctcgcg
     1441 ctcgatggcg tccacgagat cgagggggaa ttcccaaaaa accgagaagg gttccgtcaa
     1501 aatcacgccg gcgatttccc aatggccggt ggtggtgtcc ttgccggccg agcattcggt
     1561 catggttccg aacgaggcgg ccggctggcc cgcgccgggg agggagagaa tggtttccag
     1621 cccgagctgc ttcagcgcgg ggacggcgag attcggaaat gccgccagga gatgccccag
     1681 cgtgtcggcg ccggcatcgc cgtaattggc ggcatcgggc gcgtttccgc agcccgcgct
     1741 gtcgagcacc aggaggatcg cccggtgttt gggggggtaa aaaaaattgg gcaccggttg
     1801 ctatttaaag caaccgggcc caataactcc agccaagaga ttatccccta aacggcggac
     1861 ggcccctcct cgccggtccg gatccggacg gcctgatcca cggggccgac gaagattttt
     1921 ccatccccga tctttccggt cttcgcggcc ttgccgatgg cggtgaccac gggttccatc
     1981 aactcatccg tcacgaccac ctcgatcttg attttcggca ggaagtccac ggtgtattcg
     2041 ctgccgcggt agatttccgt gtggcctttc tgccggccga agcccttgac ttcgctgacg
     2101 gtcattcctt ccacccccag gtcggcgatg gcttccttta cctcctccaa cttgaagggt
     2161 ttgatgattg cttcgatttt tttcatgaaa gggtgcggtc cctgcccggg cccgtggcgg
     2221 gcccgggctc cggttccaag gttagtcgtg tttgatggtg taggatttcc agatgacggc
     2281 gacgacgatc aatgccacaa acccgatgct aatccccgca actacgggag aattagccag
     2341 gtggtggaag gcgccggtcg cgccgatgaa ttcctcggcg gttttaaagg gtgttcccat
     2401 aacgtgatgt cctttctgat tggttagtgt ttggtggcga gatggctttc gggataggca
     2461 tcgatgccga tttcgctgat gtcgagaccg tcggtttcga cttcggccga aacgcgcagc
     2521 atcttcagct tcttcaggat caggctgacg acgtagccgg ggatgaatcc cagcgggatg
     2581 cagatgagca ccgggatcag ctggcccatg aaggagatcg gcg
//
LOCUS       DATDAY010000357         2633 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_664294, whole genome shotgun sequence.
ACCESSION   DATDAY010000357 DATDAY010000000
VERSION     DATDAY010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2633)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2633)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2633
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_664294"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            482..1207
                     /locus_tag="VIS74_07110"
     CDS             482..1207
                     /locus_tag="VIS74_07110"
                     /inference="COORDINATES: protein motif:HMM:NF019242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HEY5793049.1"
                     /translation="MTQVEEHLRLVMSNRFAENGDSMGKAGFRLALFLCVAAGAARAE
                     LQFQKTELRDQIFEESGAILRAFDFQTTGEKAAQILKVTPSCGCTVADLSKKAFSPGE
                     PGKLSFRYNPEGRQGIQAVSIDVETETGGKKAATRLTYRVNVLQAVILQPEIVFWKKE
                     AAASPKEVLVRADDSLVPKSFKIEEPPRHFSAELRWDKERLLHLLQITPSQPMQPIKE
                     PIRIVMEDQTGRRFEKTLFALIR"
     gene            1310..1708
                     /locus_tag="VIS74_07115"
     CDS             1310..1708
                     /locus_tag="VIS74_07115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5793050.1"
                     /translation="MKLLFESLIVIALAALVAVASWGFRAKALSGRIHYGVPTLTRAA
                     AGRLERAGKPTLWVEVRAEGAFSKSQGHVLHLATWENDLSHLLESWTPGDVIVVTGDP
                     KFREATRKAAERLDRAGLPSVHLLAWEGAQ"
     gene            1705..2217
                     /locus_tag="VIS74_07120"
     CDS             1705..2217
                     /locus_tag="VIS74_07120"
                     /inference="COORDINATES: protein motif:HMM:NF018942.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030416 - methylamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MauE/DoxX family redox-associated membrane
                     protein"
                     /protein_id="HEY5793051.1"
                     /translation="MKPVLSPKASYALHWMARTGLGLLFFYAGIVKILDPIRFAESIK
                     AFELTGPRLTEALTFGVPALELAGGCILALGVRSLWRGAASVLAGLLGVFSAAVASAW
                     WRGLAVSCGCFGAPDSFPSNPAWWLARNALLAAVLAALVLTQSGRASGKMAKRASCRR
                     NSPGAEKNAA"
     gene            complement(2423..>2633)
                     /locus_tag="VIS74_07125"
     CDS             complement(2423..>2633)
                     /locus_tag="VIS74_07125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5793052.1"
                     /translation="VTKLKTFHLPVRLGPELDEAKLLAAMGTDKKFESGAIRFVLTPK
                     LGEAFVSSNVSRESIAEAIRFLRRS"
BASE COUNT          526 a          765 c          787 g          555 t
ORIGIN      
        1 actcatgcct ccccataagc atattccgtt ccacatcagt gtattatcat caaatatgcg
       61 ccttcacttc gctagcacac tccccccaac cgaaatcatc ccaaacccgc cttatcttaa
      121 cgccattcgg gtcaggcgac atttttaaac tgaagaaacg tttccgagca agcgatgtaa
      181 ctgttttttg ccgtgcatct tcgttccttt atcacttgcc attctgtcaa aaattgtcgc
      241 ctgcccccag tttgtgaccc ccagtctgac ctgacctcca gttcttccaa ccgccccatt
      301 gaatcgcttt tcggatatct cttttgagaa atactaacta tacggcgcag gatgggaccg
      361 tgttccgggc ggatgagcgg tcagagcttc ggctgcgagt tcgatggaat cggcaaccgc
      421 ctgactgcca gcattgacgg cgcgaccgca tcgacctcta tcgaggacgc gctcaatcac
      481 tgtgacccaa gtcgaagagc atttgcggct ggtcatgagc aatcgattcg ccgagaatgg
      541 ggattccatg ggtaaagccg gttttcgcct tgctcttttc ctgtgcgttg cggcgggagc
      601 cgcgcgcgcg gaactccagt tccagaaaac ggaattgagg gatcagattt tcgaggagtc
      661 gggcgcgatc ttgcgggcgt tcgactttca aaccaccgga gagaaggcgg cgcagatatt
      721 gaaagtcacg ccgagctgcg gatgcacggt ggcggacttg tccaagaaag ccttttctcc
      781 gggcgagccg ggaaagctgt cttttcgcta taacccggag ggccggcagg gtattcaggc
      841 ggtttccatc gacgtggaaa cggaaaccgg tggcaagaag gctgccacgc gcctgacgta
      901 tcgggtcaac gtgctccagg cggtgatcct ccagccggag atcgttttct ggaaaaaaga
      961 agcggcggcg agtcccaaag aggtgctcgt ccgggcggat gacagcttgg ttcccaagtc
     1021 cttcaagatc gaggaaccgc cccggcactt ctcagcggag ctgcggtggg acaaggagag
     1081 gttgcttcac ctcctgcaaa tcactcccag ccagccaatg cagccgatca aggagccgat
     1141 ccgcatcgtg atggaagacc agacgggccg gcgtttcgag aagacgctct ttgccttgat
     1201 tcgctaaatc gaaggggtgg ggaattgtga atgaactgga gcaaactggg agaacgagga
     1261 tggcgggttt ttctctgtga tctttgtgtc ctcctgtaaa aacgctgtcg tgaaactgct
     1321 tttcgaaagc ttgattgtga tcgccctggc cgcgctggtg gcggtggcca gttggggctt
     1381 tcgagcgaaa gccctttccg ggcgaattca ttacggggtg ccgacgctca cccgtgcggc
     1441 ggcaggccgc ctcgagcgag cgggcaaacc taccctttgg gtggaggttc gagcggaggg
     1501 cgctttttcc aagtctcagg gccatgttct ccacctcgcc acatgggaaa acgatctgag
     1561 tcatcttctg gaaagctgga ctcccggcga tgtgatcgtc gtcacgggcg atcccaagtt
     1621 ccgggaggcg acccgcaaag ccgccgagcg cctggaccgc gcggggctcc cctccgtcca
     1681 tttgcttgcc tgggagggcg cgcaatgaag ccggtcctgt cgcccaaggc aagctacgca
     1741 ttgcattgga tggcaagaac cggtctcgga ctgctgttct tctatgccgg cattgtgaaa
     1801 attctcgatc cgatccgctt cgcggaaagc atcaaggcct tcgagctgac cggtccgcgc
     1861 ctgacggagg cgttgacctt cggagtgcct gccttggagc tcgccggcgg atgcattctg
     1921 gcgctgggcg tccgctcgct ctggcgaggc gcggcatccg tgctggcagg cctgctcggg
     1981 gtcttcagcg cggcggtcgc ttcggcatgg tggcgggggc ttgccgtgag ctgcggctgc
     2041 tttggcgccc cggactcctt tccgtcgaat cccgcatggt ggctggcccg caatgccctc
     2101 ctcgccgcag tccttgcggc tctggttctc acccaaagcg ggcgggcatc cgggaagatg
     2161 gcaaagcgtg cctcatgccg gaggaattct cccggcgccg agaaaaacgc cgcatagcct
     2221 gcagacccag gcagagcctt ctggagctgc tttccgtgcg ggtttcggac gaagacaatt
     2281 ccaaccatag caagacagct gccaagtgaa ggcaaaacac aaaggcaagc ttcgccggtc
     2341 acagaccgat gctacagcaa gacaattgca gcggcggtct gtgaccgccg gagagaacgg
     2401 ccacagtgtt tgtaaaagtg cgctaacttc ggcggagaaa ccggatcgct tcggcaatgg
     2461 actcgcggct gacgttgctg gacacaaaag cctcgccgag cttcggcgtc agcacgaagc
     2521 ggatcgcccc gctctcgaat tttttgtccg tgcccatggc cgcgaggagc ttggcctcgt
     2581 ccagttccgg ccccaaacga acgggcaaat gaaacgtttt cagtttcgtg acg
//
LOCUS       DATDAY010000358         2075 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_2077895, whole genome shotgun sequence.
ACCESSION   DATDAY010000358 DATDAY010000000
VERSION     DATDAY010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2075)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2075)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2075
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2077895"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..273
                     /locus_tag="VIS74_07130"
     CDS             <1..273
                     /locus_tag="VIS74_07130"
                     /inference="COORDINATES: protein motif:HMM:NF021553.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2278 family protein"
                     /protein_id="HEY5793053.1"
                     /translation="EWEERHNLFYEFHERDRLGWLLLEWTAQARLLEVWGTPYRRKQE
                     GVHQIHSRRASCAVAEDIRGRDGALRFYFDEEREARMVFFKFCGQP"
     gene            270..>2075
                     /locus_tag="VIS74_07135"
     CDS             270..>2075
                     /locus_tag="VIS74_07135"
                     /inference="COORDINATES: protein motif:HMM:NF015714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HEY5793054.1"
                     /translation="MSPIRSWFQWLSEKIRRRRWPLAAPVLLFAAGIGAGDLNSWLPA
                     SGAAILAGLALAGALRWPVNAWLFPGIFLTGVALQSFRVQFPPPVPAEPAALIARGVV
                     IGEPRPLGASGEKFRVVCRLRAAENWAGEPLGSFGVVLVIWKGLPPPAYGDEVSVRGM
                     IRAVAPPRNPGEFDYAGWLKRRGIYAEIEPRSAADCRVESSGQGNPLIRFALDSRRWI
                     SRAITAGLDPADPAAALIRAMTLGDTEDVPASVREDFQLSGTLHVFSVSGLHIAMLAV
                     IVWQMVRLLRLPRAAQGVIVIVLIFFYALVTGWSPPSVRAALMTACFILAAMIGRPPQ
                     PFNILAGAALVILLLNPPELFNAGFQLSFIVVAAILALARAWQLGLQGWLAPDPWLPR
                     PLYSPLQEAQWLGAHHAGGLLSVSLAAWLGSAWLTGFHFHFVSFVAPLINLLVVPLSF
                     LILSLATLSLLAYGLSAWLAGIFAQSNWLAAALTLFLVERSADLPGASAYVGLPLSPA
                     TRALVIVFDAGRGGAVVIQTARQTVLLDSGNDFFFQRAVLPYLHSRGIVRLRGLLLTH
                     GDAAHAGGASRAVRELRPAWVGVAAVRSRSAALAAF"
BASE COUNT          246 a          654 c          762 g          413 t
ORIGIN      
        1 gaatgggagg agcgccacaa tctcttttac gagttccacg agcgcgaccg gctgggctgg
       61 ctgctgctgg aatggaccgc gcaggcccgg ctgctggagg tctggggcac gccctaccgc
      121 cgcaaacagg agggggttca ccagattcat tcgcgccgcg cgagctgcgc ggtggcggag
      181 gacatccgcg gaagggacgg cgccctgcgg ttttatttcg acgaagagcg cgaggcgcgc
      241 atggtctttt ttaaattctg cggccagccg tgagccccat ccgatcctgg tttcaatggc
      301 tgagcgaaaa aatccgccgc cggcgctggc cgctggcggc gcccgtcctg ctgtttgcgg
      361 cgggaatcgg cgcgggcgac ctgaattcct ggctgccggc cagcggggcg gccattctgg
      421 cggggctggc gctcgccgga gccctgcgct ggccggtgaa cgcatggttg tttcccggca
      481 tttttctgac cggggtcgcg ctgcaatcct tccgcgtcca gttcccgccg cccgtgccgg
      541 cggagccggc ggccctgatc gcgcgcggcg tggtgatcgg ggaaccccgg ccacttggcg
      601 cctcgggcga aaaattccgc gtcgtctgcc ggctgcgcgc ggcggaaaat tgggcgggcg
      661 agcctctcgg atcgtttggc gtggttttgg tgatttggaa gggtctgccg ccgccggcgt
      721 atggcgacga ggtgagcgtg cggggcatga ttcgcgcggt ggccccgccg cggaaccccg
      781 gggaatttga ttacgcgggc tggctgaagc gccgggggat ttacgccgag atcgagccgc
      841 gctcggcggc ggattgccgg gtggaatcgt ccgggcaggg caatcccctg atccggttcg
      901 cgctcgattc ccggcggtgg atttcccggg cgatcaccgc ggggctggac cccgccgatc
      961 cggcggcggc gctcattcgc gcgatgacgc tgggagacac cgaggacgtg cccgcgtccg
     1021 tgcgggagga ctttcagctg agcgggacgc tccacgtgtt ttcggtgagc ggattgcaca
     1081 tcgcgatgct cgccgtcatc gtctggcaaa tggtccggct gctgcgcctg ccgcgggcgg
     1141 cgcagggagt gatcgtcatc gtcctgattt tcttttacgc gctagtcacg ggctggagcc
     1201 cgcccagcgt gcgcgcggcg ctgatgacgg cgtgcttcat tctcgcggcg atgatcgggc
     1261 gcccgccgca gccattcaac atcctggccg gcgcggcgct ggtcattttg ctgctgaatc
     1321 cgccggagct gtttaatgcc gggtttcaac tttcgttcat cgtcgtggcg gccatcctgg
     1381 cgctggcccg ggcctggcag ctcggcctgc aaggctggct ggcgccggat ccgtggctgc
     1441 cgcggccgct ctattccccg ctgcaggagg cgcaatggct cggcgcgcat cacgcgggcg
     1501 gcctgctgag cgtgtccctg gcggcttggc tgggttcggc ctggctgacg ggctttcatt
     1561 ttcacttcgt gtcattcgtg gccccgctga tcaatctgct ggtggtgccg ctgtccttcc
     1621 tgatcctcag cctggcgacc ctttcgctgc tcgcgtatgg cctgagcgcc tggctggcgg
     1681 ggatttttgc ccaatccaac tggctcgccg cggccttgac gctgtttctg gtcgaacggt
     1741 ccgcagatct gccgggggcc tcggcttacg tggggctccc cctgtctccg gccacgcggg
     1801 cgctggtgat cgtgtttgac gcggggcgcg gaggggcggt ggtcatccag accgcccggc
     1861 agacggtgct gctggattcg ggaaacgatt tcttttttca acgcgcggta ttgccctatc
     1921 tgcacagccg cggaatcgtc cggctgcggg gactgctgct gacgcacggg gatgcggctc
     1981 acgccggcgg cgcgagccgg gcggttcggg agttgcggcc ggcgtgggtc ggggtggccg
     2041 ccgttcgaag ccgctcggcg gcgctggcgg cattt
//
LOCUS       DATDAY010000359         4058 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_1549556, whole genome shotgun sequence.
ACCESSION   DATDAY010000359 DATDAY010000000
VERSION     DATDAY010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 4058)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4058)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4058
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1549556"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3707)
                     /locus_tag="VIS74_07140"
     CDS             complement(<1..3707)
                     /locus_tag="VIS74_07140"
                     /inference="COORDINATES: protein motif:HMM:NF015377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine peptidase family C39 domain-containing
                     protein"
                     /protein_id="HEY5793055.1"
                     /translation="MLNEKPAGLASGKKGHAARKVLLLMSAGLLAAGGLLLAERSFQA
                     PPADLAASGDGVAPPVEPAPGTGEEGAETPPSDPPVVARLADGCTDEDVFHIGLFYEP
                     LVPVGGKASEQERKDFQETINAFSLSQSLELLDGFLERWPESRWRAGLEYQLGTRRYE
                     GGYFTPALAHWQKAWELSKEGREDKVHAFADQAYARLLHLNSALGRMEVLEPLLQAEK
                     ERTFLGSAWPRVQAAEGALGEMKNRPDFCFKCGPYALANVREALGMKEPLAPAITEAK
                     SGPEGFSLSQVSGLADQLGMASRMAKMETGAELPVPSVVHWKLGHYAAIVDQKEGNYL
                     VRDPTFGFENWISADAIRQETSGYFLVASEALPPGFREVGAAEGNGVYGRGLTTVQQK
                     NQTSPSDKQCPDGTCPDGMAIATVHAMMVSLHVEDTPLGYEPPFGLPINLNVFSNELE
                     SFQPGNTPNTSFGPKWSSNWSTAYIHTPGNSKASVYPPGGGAEEYPNYSDPNYRDPKS
                     QASLAYSQDASGLRQLKRNLPDSTQEVYEEKVLNSGMYFLVRITDPQGNATTLEYTGT
                     TTPLLSRIVDGPGRATTFSYEASQQPPGCPNYVTKVTDPFGRAARFYYDASGRLASIK
                     DVIGITSSFGYDGVGNVIASLTTPYGTTAFARGNGYYANERWIETTDPLGGKERVEFR
                     SGAISDNGSKNPASMVIVNGQEVNFLVDSFYLTHRNSFYWDKKKMLDGGVKADGTMDY
                     RKADLYHWLHTSNGQTSSILESIKRPGDPEKNPQGENRICYNYPGQTEAQFASNVTVA
                     KPSKIAQRIEDESTQLSQTEYNVLGLPLKKVDPLGRTTLYTYWNAARTADDPNGRDLH
                     HVKQVTAAGPGNEDIVAAYNWNSKHRPTTIKGMDGKAYTYTWNSRSQIASLTDPLNQV
                     TTYTYGAGANGSASPDGYLVSVDPPFPGTSDQITFTYDASGRVASRSQWGYTLNYQYD
                     ALDRLTRTTYPDGTYELLTYGSITQPGALDVVQERDRNGEITKYAWDANRHLTKVTDP
                     LNRDTQYEWCLCGRMTKLTDPRSRVTQWDHDLQGREISKTYADGTVWTQSYTPARGLL
                     ASRTDALNQVKTYAYALDNRLTGVSYSNAVAATPPVGWTWDAYYPRPTQMTDATGATT
                     FTYKTAGFLGGGQINAINGPWSSDTLSFTYDLLGRVATRSLNGSANQNTWTRDELGRV
                     TSVQTPLAHPSGSNTFTYSYNSANGRLAQVE"
BASE COUNT          749 a         1239 c         1176 g          894 t
ORIGIN      
        1 aattccacct gggcgagccg cccgttggcc gaattgtagc tgtaggtgaa agtattcgaa
       61 cccgacgggt gggccagcgg cgtctgcacc gaggtgaccc ggcccagctc gtcgcgcgtc
      121 caggtgttct ggttcgcgct gccgttgagc gagcgggtag ccacccggcc cagcaggtcg
      181 taggtgaagg aaagcgtgtc gctgctccac gggccgttaa tggcattgat ctggcctccc
      241 cccagaaaac ccgcggtttt gtaggtaaag gtggtggcgc cggtggcatc ggtcatctgc
      301 gtggggcgcg ggtagtaggc atcccacgtc cagcccaccg gaggcgtggc cgccacggca
      361 ttgctgtagc tcacgccggt gagccggttg tccagcgcgt aggcgtaggt cttcacctgg
      421 ttgagcgcgt cggtgcggct ggccagcagg ccgcgggcgg gcgtgtagct ctgggtccag
      481 acggtgccgt cggcgtaggt cttggagatt tcccggccct ggaggtcgtg gtcccactgg
      541 gtgacccggc tgcgcgggtc ggtcagcttg gtcatccgcc cgcagaggca ccattcatac
      601 tgcgtgtcgc ggttgagcgg atcggtcacc ttggtgaggt ggcggttggc atcccaggca
      661 tacttggtga tttccccgtt gcggtcgcgc tcctggacga cgtccagggc tcccggctgg
      721 gtgatcgatc cgtaagtcag caattcatag gtgccgtcgg gataagtcgt ccgggtgagc
      781 cggtcgagcg cgtcgtattg ataatttaga gtataacccc actgggagcg gctggcgaca
      841 cgcccggagg catcgtaggt gaaagtgatc tggtcgctgg ttcccgggaa tgggggatcg
      901 acgctcacca ggtaaccgtc ggggctggcc gagccgttgg cgccggcgcc gtaggtgtag
      961 gtggtgactt ggttgagcgg gtccgttagg ctggcaatct ggctgcggct attccaggtg
     1021 taggtgtagg ctttgccatc cattcccttg atcgtggtcg ggcggtgttt gctgttccag
     1081 ttataggcgg cgacgatatc ctcgttgccc ggacccgccg cggtgacttg cttgacgtga
     1141 tgcaagtctc ggccattggg gtcgtcggcc gtgcgggcgg cgttccaata cgtatagagg
     1201 gtcgtccggc ccaaggggtc cacctttttg aggggaaggc ccaggacatt atattcggtt
     1261 tgtgaaagct gggtactttc gtcttcgatg cgctgggcga tcttgctggg tttggcaacg
     1321 gtcacatttg aggcaaattg agcttccgtt tgccccggat agttatagca aatgcgattt
     1381 tccccctgag gatttttctc cggatccccg ggacgtttga tgctttccaa aatactggaa
     1441 gtctgcccat tagaagtatg cagccagtga tagaggtccg ctttgcggta atccatggtg
     1501 ccgtccgcct tcaccccgcc atccagcatc ttctttttgt cccaataaaa agaattgcga
     1561 tgggtaaggt agaagctgtc caccaaaaaa ttaacctctt ggccgttgac gattaccatt
     1621 gaggcgggat tctttgagcc gttgtcagat atcgctcccg atcgaaactc aaccctctcc
     1681 ttgccgccaa ggggatcagt cgtttcgatc catcgctcat tggcgtaata cccattgccg
     1741 cgggcaaagg cggtcgttcc gtatggagtg gtcagcgagg caattacatt accaacgcca
     1801 tcataaccaa aagaagaagt gatgccaatg acatccttga tactcgccag gcggcccgag
     1861 gcatcataat aaaaacgcgc agcccttcca aagggatccg tcaccttggt aacgtagttg
     1921 gggcaaccag ggggctgctg gctggcttca taactgaagg tggttgcccg gcccggcccg
     1981 tccacgatcc tcgaaagcag cggcgtcgtg gtccccgtgt attccagagt ggtggcattg
     2041 ccctgcggat cggtgatcct aaccaggaaa tacatcccgg aattcagcac tttttcttca
     2101 taaacttcct gggtgctgtc cggaagattc cgcttgagtt ggcgcaaccc cgaagcgtct
     2161 tgtgaataag ccagcgaggc ctgcgatttc ggatcgcgat agttggggtc gctatagttg
     2221 ggatattcct cggctccacc ccccggcgga taaaccgacg ctttgctgtt acccggcgtg
     2281 tgaatgtaag ccgtgctcca attcgaactc cacttcggtc caaagctggt attaggggta
     2341 ttgcccggct gaaaactctc cagttcattg ctgaagacgt tcaaattaat cggaagtcca
     2401 aagggcggct cgtagcccaa cggagtatcc tccacgtgca aactcaccat catagcatgc
     2461 accgtggcaa tggccatgcc gtcaggacaa gtcccatccg ggcattgctt gtcgctggga
     2521 ctagtctggt tcttttgttg cacagtcgtg agcccgcgcc cgtaaacccc atttccctcc
     2581 gccgcgccca cctcgcggaa tcccggcgga agcgcctcgg atgccaccaa aaaataaccg
     2641 ctggtttcct ggcggatcgc atcggcgctg atccagttct caaacccaaa ggtcgggtcc
     2701 ctcaccaggt aatttccctc cttttggtcc acgatggccg cataatgccc cagcttccag
     2761 tgcaccaccg agggcacggg cagctccgcg cccgtctcca tcttcgccat gcggctggcc
     2821 atccccagct gatccgccag cccggaaacc tggcttaagg aaaatccctc cggccccgat
     2881 ttcgcctccg tgatcgcggg cgcgaggggt tccttcattc ccagcgcctc ccgcacgttg
     2941 gccagcgcat acggtccgca cttgaagcaa aaatcggggc ggttcttcat ttcccccagc
     3001 gccccctccg ccgcctgcac ccgcggccac gccgaaccta aaaacgtccg ctctttctcc
     3061 gcttgcaata acggctcgag cacctccatg cgccccagcg cgctgttgag atgcaacaac
     3121 cgggcgtagg cctggtcggc aaaggcatgc accttgtcct cccgtccctc cttgctcagc
     3181 tcccaggctt tttgccaatg cgccaaggct ggggtaaaat aacccccttc gtaacgccgc
     3241 gttcccagct gatactccag ccccgcccgc caccggctct ccggccaccg ttccagaaaa
     3301 ccgtccagca attccagcga ttggctcagg ctgaaggcat tgatcgtttc ctgaaaatcc
     3361 ttgcgctcct gctccgaggc cttcccgccc accggaacca gcggctcata gaacaggccg
     3421 atatgaaaga cgtcttcgtc cgtgcagccg tccgccaggc gcgcgaccac cggcggatca
     3481 ctgggcggag tttccgcacc ttcttccccg gtcccggggg cgggttccac cggaggcgcc
     3541 accccgtcac cgcttgcggc caaatccgcc gggggcgcct gaaaggatcg ctccgccagc
     3601 aacaggcctc ccgccgccag caaccccgcg ctcatcagca aaagaacctt ccttgccgca
     3661 tgccccttct ttcccgatgc caacccggcc ggtttttcgt tcagcatgaa cggcctttag
     3721 ccagttgtta atcgcctgtc tattctattt tttgtttttt acataatgtt gcgcaacaca
     3781 aagaaccgtt gcttccattc tttcacagag gacgaaaaac gaatggcttc tggttgcgga
     3841 aatttccaga gcgatcatat gcatacgcgc cgaaaaaagt gagcggcaag ggcctgccgc
     3901 tctccataaa aggttggagg gcggtgggct ctcaccgccc attctatttt tctatttgca
     3961 gcaacatccg ccatccccct tcgccacctc gacgctgtgc gcggacttgg cgggcgggag
     4021 gtggatggat gggttgcgat tgaaaaagcc ggccggct
//
LOCUS       DATDAY010000360         3856 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_1549658, whole genome shotgun sequence.
ACCESSION   DATDAY010000360 DATDAY010000000
VERSION     DATDAY010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 3856)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3856)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3856
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1549658"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..462
                     /locus_tag="VIS74_07145"
     CDS             <1..462
                     /locus_tag="VIS74_07145"
                     /inference="COORDINATES: protein motif:HMM:NF025668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III-like domain-contianing
                     protein"
                     /protein_id="HEY5793056.1"
                     /translation="WFDAKKIEPLFPFGYGLSYTTFDFKNLKLSSPSLDPKGRVQISV
                     DVTNTGKRAGAEVVQLYVRDPKPAVEKAVRELKGFAKVALQPGETKTVTLALTPRDFA
                     WFDVAGMQWKATPGSYDLEIGASSRDIRQKASVNLTGLYIEPAGKSSAKSD"
     gene            477..1673
                     /locus_tag="VIS74_07150"
     CDS             477..1673
                     /locus_tag="VIS74_07150"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY5793057.1"
                     /translation="MNRLALAALCAFVPLFHSGAAEPLTAAIKTDPYVYVGQPVTLDG
                     SASTQGADVAYSWKAKGKISLQDAGKSIARFTPAQTGYNDFTLTVTRGAEQKDARAGV
                     SALNDPKNDRSPVFDGARKVLYKEAPNVLGDPAKLYLHVFNPPDWKASDQRAAVLIFH
                     GGAWKVGSPKDCVAECRYWASRGLVAIAAQYRIAEWQGTKPEDCVADARSAVRYVRAH
                     AAELGIDPQKIAVGGTSAGAHIAACAGTLPAYDDPGDDRAVSCVPNALLLYFPYSMVT
                     TAGNGRDDMSPLHFVGGATPPTLFIGGELDAIAPAVRGIEWAEKVKGLKNPFRLFIYR
                     NTRHGVGQPGMNQPGVANDVIRQTDLFLASLGFVSGEPTVPPMDAAAIEALHVDPKNL
                     KPDSKP"
     gene            1686..>3856
                     /locus_tag="VIS74_07155"
     CDS             1686..>3856
                     /locus_tag="VIS74_07155"
                     /inference="COORDINATES: protein motif:HMM:NF014026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HEY5793058.1"
                     /translation="MTIHFPSPQTIPGLLLAFLPAMAAAQSPDPAMEQAKAILAKMTL
                     EEKVSLCHGNSTMAIGAIPRVGIMEEFTMSDGPHNVRPELARDTFQSAGRDDDHSTSL
                     PPLSTLAATWDVGMAARFGDVIGKEARDRGKDMMLGPGVNIVRTPVCGRNFEYLGEDP
                     ALAAAMGVALIQAMQANDIAACVKHFAANNQELNRRGVNAEIDERTLREIYLPAFEAA
                     VKEGGVLTVMSGYNKFRGEFCSHNGYLNNQILRDQWGFKGFVVSDWGGLHGTLEGALG
                     GTDVEMNAGRAIRYFNQPLLDAVKAGQVPESLIDDKALRVLYVMAKTHKLDGEPREKG
                     ARNTPEHQRAAREIAEASIVLLKNDRPLLPLDAGSLKSVLVMGKPAIEKHAATGGSSE
                     GKPPYEITPLEGLKKRLGPGVKVSYLPLSANERGFNVTEIPASAIRTFDTEIQDAGLA
                     IPAWRAEYFNNPSLEGAPAATGFDRKLDFNWGSEPPRKGIPADKFSARWTAKIAAPET
                     GVYTLRAAGDDGFRVFVDGKTVIDNWNYPRQPEAAVEVALEAGREYEFRVEYFDDLRE
                     AAFNLQWQLPSDRGSDLAQVKALAEKAGAVLLFTGAGHVLESEGGDRPDMKLPAGQDE
                     AIAAVLGVNPRAVVINFSGSPMEMPWIDQAPAVVQYWFSGQEGGSALASVLFGDVNPS
                     GKLPVTFPVKLADSPAHALGNYNDKSVNYAEGIFVGYRWFDA"
BASE COUNT          719 a         1309 c         1209 g          619 t
ORIGIN      
        1 tggttcgacg cgaagaagat cgagccgctg tttcccttcg gctacggact gagctacacc
       61 acgtttgatt tcaaaaacct taagctctcc agccccagcc tggacccgaa gggccgggtg
      121 cagataagcg tggacgtcac caacaccggc aagcgcgcgg gcgcggaagt cgtgcagctc
      181 tacgtgcgcg atcccaagcc cgcggtcgag aaggcggtgc gcgagctgaa aggcttcgcc
      241 aaggtcgcgc ttcagcccgg agaaaccaag accgtcaccc tcgcgctcac gccacgcgac
      301 ttcgcctggt tcgacgtggc gggcatgcaa tggaaggcca cgccgggcag ctacgatctc
      361 gagatcggcg cctcctcgcg cgacatccgc caaaaggcct cggtgaatct cactggactt
      421 tatattgagc ccgcgggaaa gagttccgca aaatcggatt gattccattc ccatccatga
      481 accgattggc ccttgccgcg ctttgcgcgt tcgttcccct tttccactcc ggcgccgccg
      541 agccgctgac ggcggcgatc aaaacggatc cctatgtgta tgtcgggcag cccgtcacgc
      601 tcgacggctc cgcttccacc cagggggcgg atgtcgccta ttcctggaag gccaaaggca
      661 aaatctccct tcaggacgcc ggcaagagca tcgcccgctt caccccggcc caaacgggct
      721 ataatgattt cacgctcacc gtgacccggg gtgcggagca aaaggacgcc cgcgccggcg
      781 tttccgccct gaacgatccg aaaaatgacc gcagcccggt tttcgacggc gcgcggaagg
      841 tgctttataa agaggcgccc aacgtgctcg gcgatcccgc gaagctttac ctgcacgtct
      901 tcaatccgcc ggactggaag gcgtcggacc agcgggccgc cgtgctgatc ttccacggcg
      961 gcgcgtggaa agtgggctcg cccaaggatt gcgtcgcgga gtgccgctac tgggcctcgc
     1021 gcgggctggt ggccatcgcc gcgcaatacc gcatcgccga atggcagggc acgaaacccg
     1081 aggactgcgt ggcggatgcc cgctccgccg tgcgttacgt gcgcgcgcat gccgcggaac
     1141 tgggcatcga tccgcaaaag attgccgtgg gcggcacctc cgccggcgcc cacatcgccg
     1201 cgtgcgccgg aaccctgccc gcctacgacg atcccggcga cgaccgggcg gtcagctgcg
     1261 ttcccaacgc cctgctcctc tatttcccgt attccatggt caccaccgcg ggcaatggcc
     1321 gcgacgacat gtccccgctg catttcgtgg gcggggccac gccgcccacc ctgttcatcg
     1381 gcggggaatt ggacgccatc gcccccgccg tccggggcat cgaatgggcg gaaaaagtga
     1441 agggattgaa aaatccgttc cgtctcttca tctatcgcaa cacccgccac ggcgtcggcc
     1501 agccgggaat gaaccagccg ggggtggcga atgacgtgat ccggcagacg gatttattct
     1561 tggcttccct cggattcgtg agcggcgagc ccacagttcc gccgatggac gccgccgcca
     1621 tcgaggcctt gcatgtcgat ccgaagaacc ttaagcccga ttccaaaccc tgacccgtct
     1681 gaggcatgac catccacttt ccttcgcccc aaaccatccc gggcttgctg ctcgcctttc
     1741 tgcccgccat ggcggccgcc caatcacccg atcccgccat ggaacaggcc aaggccattc
     1801 tcgcgaagat gaccctggag gaaaaagtgt cgctctgcca cggcaacagc acgatggcca
     1861 ttggcgccat tccccgcgtc ggcatcatgg aggagttcac gatgtccgac ggaccgcaca
     1921 acgtgcggcc cgagctcgcg cgcgacacgt tccaatccgc cggccgcgac gacgaccact
     1981 ccacctcgct gccgccgctc tcgaccctgg cggcgacctg ggatgtcggg atggccgcga
     2041 ggttcggcga cgtgatcgga aaggaagccc gcgaccgcgg aaaggacatg atgctcggcc
     2101 cgggggtgaa catcgtgcgc acgccagtgt gcggccgcaa tttcgaatac ctgggcgagg
     2161 atcccgcgct ggccgccgcc atgggcgtcg ccttgatcca ggccatgcag gccaacgaca
     2221 tcgcggcctg cgtgaagcac ttcgcggcga acaaccagga gctcaaccgc cgcggcgtga
     2281 acgccgagat cgacgagcgc acgctgcgcg aaatttacct gcccgccttc gaggccgccg
     2341 tgaaggaagg cggcgtgctc accgtgatga gcggctacaa caagttccgc ggcgaatttt
     2401 gcagccacaa cggctacctc aacaaccaga tcctgagaga ccaatggggc ttcaagggtt
     2461 tcgtcgtttc cgactggggc ggcttgcacg gcaccctcga gggcgcgctt ggcggcacgg
     2521 acgtggagat gaacgcgggc cgcgccatcc gctatttcaa ccagcccctg ctcgatgccg
     2581 tgaaggccgg ccaggtgccc gaatcgctca tcgatgacaa agccctgcgc gtgctctatg
     2641 tcatggcgaa aactcacaag ctcgacggcg agccccggga gaaaggcgcc cgcaacacgc
     2701 ccgagcacca gcgggcggcg cgagagatcg ccgaggcctc gatcgtgctt ttgaaaaacg
     2761 accggcccct gctgcccctg gacgccggtt cgctcaagag cgtgctcgtc atgggcaagc
     2821 cggccatcga gaagcacgcc gcgacgggcg gaagctccga aggcaagccg ccctacgaga
     2881 tcacgccgct tgaaggcctg aaaaagcgcc tcgggcccgg cgtgaaggtt agctacttgc
     2941 cgctttccgc caacgagcgc gggttcaacg tcacggaaat ccccgcgagc gcgatccgca
     3001 cgttcgacac ggaaatccag gatgccggcc tggccattcc cgcctggcgc gcggagtatt
     3061 tcaacaaccc gtcgctcgag ggcgccccgg ccgccaccgg cttcgaccgc aagctcgatt
     3121 tcaactgggg ctccgagccg ccgcgcaagg gcattcctgc ggacaaattc tccgcccgct
     3181 ggacggcgaa gatcgccgcg ccggagacgg gcgtttacac gttgcgcgcc gcgggcgacg
     3241 acggtttccg cgtgttcgtg gacggcaaaa ccgtgatcga caactggaac tacccgcgcc
     3301 agccggaggc cgccgtggag gtcgccctgg aggcgggcag ggaatacgag ttcagggtgg
     3361 aatatttcga cgatctccgc gaagccgcct tcaacctcca gtggcaattg ccgtcggacc
     3421 gcggctcgga cctcgcgcag gtcaaggccc tggccgaaaa ggccggcgcg gtgcttctct
     3481 tcaccggcgc gggccacgtg ctggagtccg agggcggcga ccgcccagac atgaagctgc
     3541 ccgccggaca ggatgaagcc attgcggcgg tgctcggcgt gaatccccgc gccgtggtca
     3601 tcaacttcag cggctcgccc atggaaatgc cctggatcga ccaggccccg gcggtggtgc
     3661 aatattggtt ctccgggcag gagggcggca gcgcgctggc gtcggtcttg ttcggcgacg
     3721 tgaatccgtc cggcaagctg ccggtcacct tccccgtgaa gctggccgac agcccggcgc
     3781 atgcgctcgg caattacaat gacaagagcg tgaactacgc ggaagggatt tttgttggct
     3841 accggtggtt cgacgc
//
LOCUS       DATDAY010000361         3253 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_1900702, whole genome shotgun sequence.
ACCESSION   DATDAY010000361 DATDAY010000000
VERSION     DATDAY010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 3253)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3253)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3253
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1900702"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..914)
                     /locus_tag="VIS74_07160"
     CDS             complement(<1..914)
                     /locus_tag="VIS74_07160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5793059.1"
                     /translation="MPAHSITLALMPLENLSNSPEDALLARGFLHDLIAELAQFPLLG
                     VIAADSVLSESKREAGDEAGFAERFGANYVLRGSVCRWGEILRINVQLAEMPGGHHVW
                     AGRYDSGNLLEAHDDIVAKVANALAAQVDRSILRSKPSVRETKLESYACWLRGMECLQ
                     RGTADSDMEGRHFFERALEIDANDARAHGGLSLSYFNDWSCQAWECWGENEQRAYDCA
                     RRAEALNPDDALVQIILVRVEQYRREFARAAPRLERVQALAPNDAYVLIQLAVAWTFQ
                     GDAAAGWHFARRALALNPLCPAWYYCFTA"
     gene            1236..1661
                     /locus_tag="VIS74_07165"
     CDS             1236..1661
                     /locus_tag="VIS74_07165"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HEY5793060.1"
                     /translation="MPLSPEEMDRKLDEHFAYEGGDDVEGVLATLAQDATHDVVGSPL
                     GPLRGREAARAFYEALFADLSESKVTTLRRLYGDGFLVDESLWQGRAPGRPFGLEGRN
                     RPLAFRLLHVIEFAGGGQIQKEQVWLDVASILRQLPQEE"
     gene            1658..2260
                     /locus_tag="VIS74_07170"
     CDS             1658..2260
                     /locus_tag="VIS74_07170"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY5793061.1"
                     /translation="MKVERKPSNQELRTRKDLLLAARQLMTQGRKPTLEQVAKAAMVS
                     RATAYRYFPNIDALLVEVPIDAAVGNPGEMFANDPSTDAEARVDDAEASMHQVVYENE
                     AQLRILLANSIAHGAGRDAPPNRQGRRLALIEAALATSRRRFREEDYETLCSALAMIF
                     GPESMIVFRDVIRADERTARRVKSWAVRALVRAALASSEP"
     gene            2379..2756
                     /locus_tag="VIS74_07175"
     CDS             2379..2756
                     /locus_tag="VIS74_07175"
                     /inference="COORDINATES: protein motif:HMM:NF016854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HEY5793062.1"
                     /translation="MPYDQNLAGRVSRAFEQKGATFETKSMMGGLCFMVDGKMCAGIT
                     GDLLMARIDPAEEAAALKRTGCKPMDFTGRPMKGFVFIEPTGTRTAAQLAAWLNLALE
                     FNPKAKASKKRAGKLKKSAGDPP"
     gene            2753..>3253
                     /locus_tag="VIS74_07180"
     CDS             2753..>3253
                     /locus_tag="VIS74_07180"
                     /inference="COORDINATES: protein motif:HMM:NF019614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HEY5793063.1"
                     /translation="MKTKLVYLLLFRGVGGATQLPAAPLREALTVAGFEKVATYINSG
                     NAVVRSGLPRAKVIATVARICEEKFGFTKAIFAPTLEEWSDLVGNNPFPQAVDAPKYL
                     HAALLAERPVAANVKAVNAFARPEEGFAIIGNVAYLHTPGGFGTSKLAEKFDKGIGVS
                     NTARNWN"
BASE COUNT          642 a          994 c         1030 g          587 t
ORIGIN      
        1 gcggtaaagc agtaatacca tgccgggcac agcgggttca gggcgagcgc ccgccgggcg
       61 aaatgccatc ctgcggcggc atcgccttga aacgtccagg ccacggccag ttggatgagg
      121 acataggcgt cgttcggagc cagagcctgc acccgctcca gccggggcgc ggcccgcgca
      181 aattcccggc ggtattgttc gacgcgcacc aggatgattt gcaccagggc atcgtccggg
      241 ttgagagctt ccgcccgccg ggcgcaatca tacgcgcgct gctcgttttc cccccagcat
      301 tcccaggcct ggcagctcca gtcgttaaaa tacgaaagcg acagtccgcc gtgggcgcgc
      361 gcatcgttgg catcgatctc caacgcccgt tcgaaaaaat ggcgcccttc catgtccgag
      421 tccgccgtgc cgcgctgcaa acactccatg ccacgcagcc agcaggcgta ggactcgagc
      481 ttcgtctcgc gaacggacgg cttggaacgc aaaatcgacc ggtccacctg tgccgccagc
      541 gcattggcca ctttggcgac gatatcatca tgggcctcca acagattccc gctgtcgtag
      601 cggccggccc agacgtgatg gccgccgggc atttccgcga gttggacgtt gatccgcaat
      661 atttctcccc aacggcacac gctcccgcgg agcacgtagt tagccccaaa gcgttcggca
      721 aatcccgcct catcgcccgc ttcccgtttg ctctcggaga gcacggaatc ggcggcgatc
      781 acccccagga gcggaaactg ggcgagttca gcaatcagat cgtgcaaaaa accgcgtgcc
      841 agcaaggcgt cctccggcga gttggaaaga ttttccaacg gcatcaaagc cagggtaatg
      901 gaatgcgcgg gcatggcggc ggatagtcaa ccgcctcagc gttgctcgct tctggaatct
      961 ggcaagcacg aaataggcgc gcctggcttt ccctcatgcg gacggcgggc ttgccgccag
     1021 gccgctgccg gagaccgatg cgggaattgg ccggcgagct ggcgaaagcg cggctgaggc
     1081 aggccgcccg ccggcttggc gtcacgcgcc ctcaatttcc gggccgccgc tctccgcaaa
     1141 ggtccggcaa ttttaaaaca aaaatcttga aatgagacat tcgtctcatt aaagcgttcc
     1201 ttaaccgcag aaaataacct ttaatggaga tcatcatgcc gttgtcaccc gaggaaatgg
     1261 accggaagct ggacgagcat tttgcttacg agggagggga cgatgtggaa ggggttctgg
     1321 ccacgctggc ccaggatgcc acgcatgacg tcgtcgggtc gccgctgggt cccctgcgtg
     1381 gccgggaggc ggcgcgggcg ttttatgaag cgctgtttgc cgatttatcg gaaagcaagg
     1441 tgacgacttt gcggcggctt tatggcgacg gctttctggt ggacgaatcc ttgtggcagg
     1501 gtcgggctcc cggacggccg ttcggtttgg agggccgaaa ccgcccgctt gctttccgcc
     1561 tccttcacgt gatcgagttc gccggcggcg gccagattca aaaagagcag gtatggctcg
     1621 acgtggcgtc gattctccgg caactgcctc aggaggagtg aaggtggagc ggaagccgag
     1681 caaccaggag ttgcggacgc gcaaggatct gctgctcgcg gcccgtcagc tcatgacgca
     1741 gggccgcaag cccacgctgg agcaggtggc aaaggcggcg atggtttccc gggcgacggc
     1801 gtatcggtat tttccaaaca tcgatgcgct gttggtggag gtgcccatcg acgcggcggt
     1861 gggaaatccc ggggaaatgt ttgcgaacga cccatccacc gatgcggagg cgcgggtgga
     1921 cgacgcggaa gcttccatgc accaggtcgt ctatgagaac gaagcccagt tgcgaatcct
     1981 gctcgccaac tccatcgccc acggcgccgg gcgcgatgcg ccgcccaatc ggcagggccg
     2041 gagactcgcg ttgatcgaag cggccctggc gacgtcgcgg cgccggtttc gcgaggagga
     2101 ctacgagacg ctgtgctcgg ccctggcgat gatcttcgga ccggaatcga tgattgtctt
     2161 tcgcgacgtc attcgcgccg atgagcggac ggcgaggcgc gtcaagagct gggcggtgag
     2221 agccctggtt cgcgcggccc tcgcgtcctc ggagccttaa gcttgaccgg ccatgcgatt
     2281 tatcacgcgg agccgctaag atcgtcccgc ctttattcag ccattgacga tccctccggt
     2341 tccccggaaa tataaccttg gcttctttcc gttgcattat gccatacgat caaaatctcg
     2401 ccggccgcgt gagccgtgcg tttgaacaaa agggtgccac cttcgaaacg aagtcgatga
     2461 tgggcggcct gtgtttcatg gtggatggaa agatgtgcgc cggtatcacc ggcgatcttc
     2521 tcatggcccg catcgacccc gccgaggaag cggcggcgct gaagagaacc ggttgcaaac
     2581 ccatggactt tacgggccgg ccgatgaagg gcttcgtgtt catcgaacca accgggactc
     2641 gcacggccgc gcaactcgct gcctggctga acctcgcgct tgagttcaac cccaaggcaa
     2701 aggcgtcaaa aaagcgtgct ggcaagctca agaaatccgc cggtgacccg ccatgaaaac
     2761 caaattagtc tatctcctgc tgtttcgcgg cgtcggcggc gccacgcagt tgccggctgc
     2821 gccactgcgc gaggcgctga ccgttgccgg attcgaaaaa gtcgcgacct acatcaacag
     2881 cggcaatgcc gtggttcgca gcgggctgcc ccgcgcgaag gtgatcgcca cagtcgccag
     2941 gatctgcgag gagaaattcg gcttcaccaa ggcgatcttc gcgccgactc tggaagagtg
     3001 gtctgatctc gtcggcaaca acccgttccc gcaagccgtg gatgcgccaa aatacctgca
     3061 cgcggcctta ttggcggagc gtccggtggc ggcgaatgtc aaagccgtaa acgcgtttgc
     3121 caggccggaa gagggtttcg cgatcatcgg caacgtggcg tatctgcaca cgccgggcgg
     3181 attcggcacc tcgaagctgg ccgagaaatt tgacaaggga atcggcgtga gcaacaccgc
     3241 ccgcaactgg aac
//
LOCUS       DATDAY010000362         2350 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_2079043, whole genome shotgun sequence.
ACCESSION   DATDAY010000362 DATDAY010000000
VERSION     DATDAY010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2350)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2350)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2350
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2079043"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..347)
                     /locus_tag="VIS74_07185"
     CDS             complement(<1..347)
                     /locus_tag="VIS74_07185"
                     /inference="COORDINATES: protein motif:HMM:NF024740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5793064.1"
                     /translation="MNSIRGILFDLDGTLYVGDELIPGAREALEWCARRELPVRFVTN
                     TTSKPRREIVEKLGRLGIRVPVDHLFTAPAAAREVLLKRQWTRCHLLLRESLLDDLSG
                     IAAVNESPQAVVIG"
     gene            377..739
                     /locus_tag="VIS74_07190"
     CDS             377..739
                     /locus_tag="VIS74_07190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2237 domain-containing protein"
                     /protein_id="HEY5793065.1"
                     /translation="MPTNVLGQELACCCRRPMTGFYRDGYCRTGPGDFGLHTVCAVMT
                     EEFLQFSYARGNDLVTPMPEHDFPGLEPGDKWCLCVERWQEALEADVAPPVVLAATHA
                     SALEFVSLEDLRAHAAEA"
     gene            1111..1566
                     /locus_tag="VIS74_07195"
     CDS             1111..1566
                     /locus_tag="VIS74_07195"
                     /inference="COORDINATES: protein motif:HMM:NF014439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5793066.1"
                     /translation="MPQDHPTKTIFSGVFERALDEKNRVTIPAKWMTEGEACFYLAPG
                     AQKKFLMVLPPAEFIGIENRIMEGSTKSLAEKRAAVQHFQRATEEANVDKQGRMLLPE
                     RLTKEFDLSKDVMLIGNKERFEIWNTQKWQEFEQANAAIFEAVAEEIGL"
     gene            1775..>2350
                     /gene="rsmH"
                     /locus_tag="VIS74_07200"
     CDS             1775..>2350
                     /gene="rsmH"
                     /locus_tag="VIS74_07200"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: protein motif:HMM:TIGR00006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEY5793067.1"
                     /translation="MAYHTPVMPDEVAELLRPAPGMLILDGTLGGGGHTELLLKAGAN
                     VIGLDRDADAIAEVTQRLSGYARHLRTAQANFAEVEAVLEGMGVDQLGGALLDLGVSS
                     HQLDEGARGFAFMKNGPLDMRMDCRQSLTAADIVNHAPFEELARIFREYGEEPKAARI
                     ASRLVAVRIKKPLETTFDLTAAVESVIHRTGP"
BASE COUNT          494 a          706 c          727 g          423 t
ORIGIN      
        1 ccgatcacca ctgcctgcgg gctttcgttg accgccgcaa tgccggaaag atcatcgagc
       61 agcgattcgc gcagcagcaa atggcagcgc gtccactggc gcttgagcaa cacctcgcgc
      121 gcggcggcgg gcgcggtgaa gagatgatcc accggaacgc gaatgccgag gcggccgagt
      181 ttttccacga tctcgcgccg gggcttgctg gtcgtgttcg tgacaaagcg gaccggcagc
      241 tcgcggcgcg cgcaccactc caatgcctcc cgcgcgcccg gaatcaactc atccccgaca
      301 tacagcgtgc catcgagatc aaagaggatt ccgcgaattg agttcacacc tccctccggc
      361 cagctagaat catgccatgc ccaccaacgt gcttggccag gaactcgcgt gctgctgccg
      421 ccggccgatg accggatttt accgcgacgg ttattgccgc accggcccgg gggatttcgg
      481 gctgcacacg gtctgcgcgg tgatgacgga ggaatttctg cagttcagct acgcgcgcgg
      541 caatgacctg gtcaccccga tgcccgaaca cgattttccc ggcctcgaac cgggggataa
      601 gtggtgcctt tgcgtggagc gctggcagga ggcgctcgag gccgatgtcg cgccgcccgt
      661 ggtcctggcc gccacgcacg cctcggcgct ggaatttgtc agcctcgagg atctgcgcgc
      721 ccacgcggcc gaagcgtaag cggggaatcg caaaaccggc ggacccgatt ttttaccgcg
      781 gatttcgcgg atgggcgcgg ataaaaatgc cgaaatccgc ggtttaattc cgagagagca
      841 ggcggtgcgg ggaaccgctt ttgggtccga tcctccgccc aggcccggcg cggcggagcg
      901 cattcgccga gagcccggaa ttggccgcct ccgctcaaaa tcagcgaacg gatttaactg
      961 aagttttgcg gccattttcg ccctggcagc aacaaggtta tgcaaccgtc gctttgttcc
     1021 tgaaaaaccc ggttttatat tgaccaaagt ggaggaaaat ggcatagggt ggagcccgat
     1081 ggggaataat gtctaagttt ccttaattac gtgcctcaag atcatccaac caagaccatt
     1141 ttttcgggcg tctttgaacg ggcgctggat gagaaaaatc gtgtcacgat tccggccaaa
     1201 tggatgacgg agggcgaggc gtgtttttat ctcgctcccg gcgcgcagaa aaaatttctg
     1261 atggttctgc cgcccgcgga gtttatcggg attgaaaatc ggatcatgga aggctccacc
     1321 aagagcctgg cggaaaagcg cgcggcggtt cagcactttc aacgcgcgac cgaggaagcc
     1381 aatgtggaca agcagggccg catgctgctg ccggaacggc tcacgaagga atttgacctc
     1441 tcgaaggacg tgatgctgat cggcaacaag gaacgttttg agatttggaa tacgcagaag
     1501 tggcaggaat tcgaacaagc gaatgccgcc atcttcgaag cagtggccga ggagattgga
     1561 ttataggcca tgaacccact tcaacccaac ccataggagg aacgaatggc gcaacgaagt
     1621 cggaaatatt tttacggttt cacttccgcc aaagacgcct ccggcaagcc tctcggccac
     1681 tccgccctga aaattaccat taaaaacgct tatgaactta tgggattgcg caaacccgcc
     1741 tctggcagtt tggggaaacc ctgaactgga caccatggcc taccacacgc ccgtcatgcc
     1801 cgacgaggtg gcggaattgc tgcgccccgc gccgggaatg ctcattctcg acggcacgct
     1861 cggcggcggc ggacacaccg aactgctcct caaagccggg gcgaacgtga tcgggctgga
     1921 tcgggacgcg gacgcgatcg ccgaagtcac gcagcggctt tccggctacg cccggcacct
     1981 gcgcaccgcc caggcgaatt tcgcggaagt cgaggccgtg ctggagggaa tgggcgtgga
     2041 ccagctcggc ggcgcgttgc ttgacctggg agtctcctcc caccagctcg acgaaggcgc
     2101 ccgcgggttt gcgttcatga aaaacgggcc gctggacatg cggatggatt gccggcagag
     2161 cctgaccgcc gcggacatcg tgaaccatgc gccgttcgag gaactcgcgc gcattttccg
     2221 ggaatacggg gaggaaccca aggcggcgcg catcgcctcg cgcctggtgg ccgtccgcat
     2281 caagaaaccg ctggagacga ccttcgatct gaccgcggcc gtggagtcgg tcattcaccg
     2341 caccggcccg
//
LOCUS       DATDAY010000363         2850 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_2223199, whole genome shotgun sequence.
ACCESSION   DATDAY010000363 DATDAY010000000
VERSION     DATDAY010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2850)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2850)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2850
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2223199"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(201..479)
                     /locus_tag="VIS74_07205"
     CDS             complement(201..479)
                     /locus_tag="VIS74_07205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006983630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SWIB/MDM2 domain-containing protein"
                     /protein_id="HEY5793068.1"
                     /translation="MPKTENKPAGKANAAFLKPVQPDDILAAVVGSTPLPRTELTKKL
                     WDYIKKHQLQDEKKKTLINADEALAAVFNGKQQVSMFEMTKLVSSHLK"
     gene            672..1946
                     /locus_tag="VIS74_07210"
     CDS             672..1946
                     /locus_tag="VIS74_07210"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEY5793069.1"
                     /translation="MARPAAEFEEAFAQRLLVSEGRRARLMAWLFSGLVVLAAGFNLL
                     YRQLGAHLAAMDGVLLSFVLAAGYEWLAWGMFRRLAARRQQPPPWRFYANALVEASLP
                     TLTLVVLLNFLPPMIVFTSPVTYLYFLIIMLAALRLDFRLCLFSGLVAAAGYAGLVIW
                     KLPEIFAFSNPAILQMRVNLGMRAVLFVAAGGMAGLVSRVIYANLRETMRGLEERQRI
                     VNLFGQHVSPQVVNQLLTQPREAASAQREVCVLVLDIRKFTTYSEDHAADEVVAYLNT
                     LWGALVRIVNAHQGVVNKFLGDGFLAVFGVPIESENYCEKAAAAAREILAEVRRLEAD
                     GKIPPTRVGLALHAGPAIVGNIGSEERKEYTVIGDVVNVAFRIEALNKDLGSRLLVSA
                     PVRERAGLAEGERMEPMPIRGRREPVELFRLA"
     gene            1992..2843
                     /gene="purU"
                     /locus_tag="VIS74_07215"
     CDS             1992..2843
                     /gene="purU"
                     /locus_tag="VIS74_07215"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HEY5793070.1"
                     /translation="MSELAILKIDCPDAVGLLARFTGFVAERGGNFVEVNQYTDPLSR
                     WFFARLAFEPGARRLADYERDFAGLAAELRVTWSLRSSAHRVRTAILVSREDHCLADL
                     LWRWRSRELQFDLALVIGNHETCRELAEREGLPFAHQPMRPEDKEANFQRLAAALREA
                     RIEFVILARFMQILPAWFCAEFAGRVMNIHHSFLPAFAGAFPYRRAYERGVKLIGATC
                     HYATDELDAGPIIEQEVVRVEHYHDPDDLKRLGRDCEKIALARGVRYHLEDRVLLHGS
                     RAVVFRD"
BASE COUNT          452 a          844 c          978 g          576 t
ORIGIN      
        1 ggagcggcgc cctccatttt gcgtcagctt gaatcaaacc cgctccaaag ccagaccaag
       61 cctcacgcaa agccgcaaag gcgcgaagat ttcggggaag cggctgccgc tttgcgcctt
      121 cgcggctttg cgtgagatga gcccaaaaaa agaccgcccg ccggggaagg cggacggtcc
      181 aataaataag gagctaaact ttatttgaga tggctggaga ccagcttggt catctcgaac
      241 atgctgactt gctgcttgcc gttgaagacg gcggcgagcg cttcatcggc attgatgagg
      301 gttttctttt tctcgtcctg aagctggtgc ttcttgatgt agtcccagag cttcttggtc
      361 agctccgtgc ggggaagcgg agtggaacca accacggcgg ccaggatgtc atcaggttga
      421 acgggtttga ggaaggcggc gttggccttc ccggcgggtt tgttttcggt tttaggcata
      481 aggtttgggt tgaaagtaag gcgcgatcaa aatcggccgg cggatgggat gagggtcgcc
      541 gttgggaggg cgttttgccg cttttcaggg tttttggggg atttgtcgag gcccggttcc
      601 aaatttttgc gggggaggag ggccgggatt tggagttgct tccctcgcgc gccgccagcg
      661 aggatgcgcc catggcccgt cccgccgccg aatttgagga ggctttcgcc cagcgcctgc
      721 tggtcagcga gggccgccgg gcgcgcctca tggcgtggct gttcagcggg ctggtggttc
      781 tggccgccgg atttaatctg ctctaccggc agctcggggc gcacctggcg gcgatggatg
      841 gggtgctcct cagctttgtg ctggcggccg gttacgaatg gctggcctgg gggatgttcc
      901 gccgcctggc cgcccgtcgg caacagccgc cgccgtggcg attttatgcc aatgcgctgg
      961 ttgaagcctc cctgccgacc ctcacgctgg tggtcttgct gaatttcctg ccgccgatga
     1021 tcgtgtttac gagtccggtc acctatctgt atttcctgat catcatgctg gcggcgctgc
     1081 ggctggattt tcgcctctgc ctgttttccg gcctggtggc cgcggcgggc tatgcgggcc
     1141 tggtgatttg gaagcttccg gaaatttttg ccttcagcaa tcccgccatc ctccaaatgc
     1201 gggtcaacct cggcatgcgc gccgtgctct ttgtcgccgc gggcggaatg gccggattgg
     1261 tgagccgcgt gatttacgcc aatctgcggg aaaccatgcg gggcctggaa gaacggcagc
     1321 ggatcgtgaa tctcttcggc cagcacgttt cgccgcaggt cgtgaatcaa ctgctcaccc
     1381 agccgcggga ggccgcatcg gcgcagcgcg aggtgtgcgt cctggtattg gacatccgca
     1441 agttcacgac ctattccgaa gaccacgccg ccgatgaggt ggtggcttat ctcaacacgc
     1501 tgtggggcgc gctcgtgcgg atcgtcaacg cgcaccaggg cgtggtgaac aaatttctgg
     1561 gcgacggttt tctggcggtg ttcggcgtgc cgatcgagtc ggagaattat tgtgaaaagg
     1621 cggcggccgc ggcgcgggag atcctggcgg aagtccggcg gctcgaggcg gacggaaaaa
     1681 ttccgcccac gcgggtgggg ctcgcgctgc acgccggccc ggccattgtc ggaaacatcg
     1741 gctccgagga acgcaaggaa tacacggtga tcggcgacgt ggtgaacgtc gccttccgca
     1801 tcgaggcgct caacaaggac ctgggttcgc ggctcttggt ctcggcgccg gtgcgcgagc
     1861 gggccgggct ggccgagggg gagcgaatgg agcccatgcc cattcgcggc cggcgcgaac
     1921 ccgtcgagct tttccgcctg gcgtgaaaaa gaaatgggac tgctcccgcc cgccatggta
     1981 aatccacggc gatgagcgag ctggccattc tcaaaattga ctgcccggac gcggtcgggc
     2041 tgctggcgcg cttcaccggc tttgtcgccg aacgcggcgg caatttcgtc gaggtcaatc
     2101 aatacaccga tccgctcagc cgctggttct tcgcccggct ggccttcgag ccgggcgcgc
     2161 gccgcctcgc ggactacgag cgcgattttg ccggactggc cgcggaactg cgggtgacct
     2221 ggagcctgcg gtcctcggcg caccgcgtcc gcacggccat cctggtcagc cgggaggacc
     2281 actgcctcgc cgacctgctc tggcgctggc gttcgcggga gctgcagttc gatctcgccc
     2341 tcgtgatcgg caatcacgaa acctgccggg agctggccga gcgcgagggg ctcccgttcg
     2401 cgcaccagcc gatgcgcccg gaagataagg aggcgaattt tcagcggctg gcggcggccc
     2461 tgcgcgaggc gcggattgag ttcgtgatcc tcgcgcggtt catgcagatc ctgccggcgt
     2521 ggttttgcgc ggagttcgcc gggcgcgtga tgaacatcca ccattccttc ctgccggcat
     2581 tcgcgggcgc gtttccctac cgccgcgcct acgagcgcgg cgtcaagttg atcggggcca
     2641 cgtgccatta cgccacggac gaactcgacg cgggtccgat catcgaacag gaggtcgtgc
     2701 gggtggagca ttatcatgat cccgatgatc tcaagcgcct cgggcgggac tgcgaaaaaa
     2761 tcgccctggc gcggggcgtg cggtatcatt tggaagaccg cgtgctgctg cacggctccc
     2821 gcgcggtggt gttccgcgac taggcgccga
//
LOCUS       DATDAY010000364         2399 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_504396, whole genome shotgun sequence.
ACCESSION   DATDAY010000364 DATDAY010000000
VERSION     DATDAY010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2399)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2399)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2399
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_504396"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..499
                     /locus_tag="VIS74_07220"
     CDS             <1..499
                     /locus_tag="VIS74_07220"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HEY5793071.1"
                     /translation="GVVIGSDGFGYELVNGRRLKIPQTGIVQVDDDVEIGANSTVDRA
                     RFGKTWIQEGVKIDNLVQIGHNVTVGAHTVLCAHVGISGSTKLGNYVTMAGKAGVNGH
                     IEIGDGAIITAMAGVTKSVPAGEVLVGLPARPMKEYKENYFQLHNIHKLYARVKKLEE
                     KTKPD"
     gene            complement(583..873)
                     /locus_tag="VIS74_07225"
     CDS             complement(583..873)
                     /locus_tag="VIS74_07225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5793072.1"
                     /translation="MTLEAIRHYLEATPFVAFRLITASGKKHYVPGPGFLHFSPSGKT
                     ANVYAKDGESFASLDVALITEILPDKRPAKRKKAEAETAPAGEPEATEDFSN"
     gene            951..2093
                     /locus_tag="VIS74_07230"
     CDS             951..2093
                     /locus_tag="VIS74_07230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HEY5793073.1"
                     /translation="MALEIATMFDPRFETLARVLVRHSTQLQPGERVLIDAFDIPDEM
                     VIALIREIRARKGLPFVNVHRARISRELTLGATDEQAELSAGISLAQMKQMQAYIALR
                     GSHNITEMSDVPEERMRVVLAKTRPVLDYRINQTKWVVLRWPTPAMAQQAQMSTEAFE
                     DFYFKVCTLDYARMAPGMKALKELMDQTDRVQLKGPGTDLRFSIKGIGSEVCGGDRNI
                     PDGEVFSCPVKDSVEGHVTYNAGTIYQGTAFDGIRLGFAKGKVVEAAAGSASQTKRLN
                     EILDTDAGSRYIGEFALGFNPHIRHPMRDILFDEKIAGSFHFTPGQAYERAGNGNKSQ
                     IHWDMVNIQRKDYGGGEVWFDDRLIRKDGLFVLPALKKLNPDYLAK"
     gene            complement(2200..>2399)
                     /locus_tag="VIS74_07235"
     CDS             complement(2200..>2399)
                     /locus_tag="VIS74_07235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5793074.1"
                     /translation="TGTLGNYAVIGVVSGKKLCLLANSDGWIYYTVVLEKTSAGILAG
                     HYSEKVPFSTQDQQEILLKRW"
BASE COUNT          491 a          762 c          700 g          446 t
ORIGIN      
        1 cggcgtggtc atcggctcgg acggcttcgg ttacgagctg gtcaatggcc ggcgcctcaa
       61 gattccgcag accgggatcg tgcaggtgga cgacgacgtc gaaatcggcg ccaacagcac
      121 cgtggaccgc gcccgcttcg gcaagacctg gattcaggag ggcgtaaaga tcgacaacct
      181 cgtccagatc ggccacaacg tgaccgtcgg cgcccacacg gttctctgcg cgcatgttgg
      241 catctccggc agcaccaagc tcggcaacta cgtcaccatg gccggcaagg ccggcgtcaa
      301 tggccacatc gagatcgggg atggcgccat catcaccgcc atggccgggg tgaccaagag
      361 cgtgcccgcc ggcgaagtgc tggtcggcct gcccgcccgg ccgatgaagg aatacaagga
      421 aaactacttc cagctgcaca acatccacaa actttacgcg cgggtgaaga agctggagga
      481 gaaaactaaa ccggattgac tggatttacc ggattaacgg gatcggaaga gtcattaatt
      541 tcctaaaaat cccgttaatc cggcaaatct tgttaatctc gtttagttcg aaaaatcctc
      601 cgtcgcctcc ggctcccccg ccggcgcggt ttccgcctcc gcctttttcc gcttcgcggg
      661 gcgcttgtcg ggcaggattt cggtgatcaa cgccacgtcc aggctggcga aactttcccc
      721 gtccttcgca tacacgttcg cggtctttcc cgacggcgaa aaatggagga atcccggtcc
      781 cggcacgtag tgctttttgc ccgacgcggt aatgagccga aaggcgacaa acggagtcgc
      841 ttccaggtaa tgacgaatgg cttcgagggt catcgttccg ctttcttaac ccatggcttc
      901 ggccaaggca attccgttgt cgggacttgc gttttcgcct cggctgggtg atggcattag
      961 agatagccac catgtttgat ccccgcttcg aaaccctcgc ccgcgtcctg gtccgccact
     1021 ccacccaact tcaacccggt gaacgggtgc tcattgatgc gtttgacatc ccggacgaga
     1081 tggtcatcgc gctgatccgc gagattcgcg cccgcaaggg ccttccgttc gtgaatgtcc
     1141 accgcgcgcg catttcccgc gaactcacgc tgggcgccac cgacgagcag gccgagctca
     1201 gcgccggcat ttccctggcg cagatgaagc agatgcaggc ttacatcgcc ctgcgcggca
     1261 gccacaacat caccgaaatg tccgatgtgc ccgaggagcg gatgcgcgtc gtgctcgcca
     1321 agacgcggcc cgtgctcgac taccgcatca accaaaccaa atgggtcgtg ctgcgctggc
     1381 cgacccccgc catggcccag caggcgcaga tgagcacgga ggcgttcgag gatttttatt
     1441 tcaaggtctg cacgctcgac tacgcgcgca tggcgcccgg catgaaggcg ctgaaggaac
     1501 tcatggacca gaccgaccgc gtccagctca aggggcccgg caccgacctg cggttcagca
     1561 tcaagggcat cggttcggaa gtctgcggcg gggaccgcaa catccccgac ggcgaggttt
     1621 tctcctgccc ggtcaaggac agcgtggaag gccacgtgac ctacaatgcc ggcacgattt
     1681 accagggaac cgcgttcgac ggcatccgcc tcggctttgc gaaaggcaag gtcgtcgaag
     1741 ccgccgccgg cagcgcgagc cagacgaagc gcttgaatga aatcctcgat accgacgcgg
     1801 gttcgcgtta catcggcgaa ttcgcgctgg ggttcaatcc gcatatccgc catcccatgc
     1861 gcgacatcct cttcgacgaa aagatcgccg gttcgttcca cttcaccccc ggccaggcct
     1921 acgaacgcgc cggcaacggc aacaagagcc agatccactg ggacatggtc aacatccagc
     1981 gcaaggacta cggcggcggc gaggtgtggt tcgacgaccg gctgatccgc aaggacgggc
     2041 ttttcgtcct ccccgcgctc aagaagctga acccggatta tctcgcgaaa tagagcgatt
     2101 ctcgaaagag ttttccgaaa tgcccaacgc gttgaggcgc ttgtcgggtg ggaaaatggg
     2161 gtccccgcgc ccgggcgtta atcacccgcg ccgttccggc taccagcgtt tcaacagaat
     2221 ctcctgctga tcctgagtgc tgaagggcac tttttccgag taatggccgg ccagaatgcc
     2281 cgcggaggtt ttttccaaaa ccaccgtgta ataaatccag ccgtccgagt tcgccaggag
     2341 gcagagtttt ttgccgctga ccacgccgat caccgcgtag ttgccgagcg tcccggtaa
//
LOCUS       DATDAY010000365         5103 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_2532365, whole genome shotgun sequence.
ACCESSION   DATDAY010000365 DATDAY010000000
VERSION     DATDAY010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 5103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5103
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2532365"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1719)
                     /locus_tag="VIS74_07240"
     CDS             complement(<1..1719)
                     /locus_tag="VIS74_07240"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY5793075.1"
                     /translation="MKILVVDDEPGLASGLAAILEQAGYAEVKAVVSIDEAYAQVTAW
                     NKLDLLITDVILNPEDGFALREVLQSQLPALRTIFISGYDLTDYDDRLAGALFLPKPL
                     DPGTLVAVVRQMIGAPVPRPAVAAAPVAVAQPVAVPEAVATPVAVVPQVVPTPTPRAT
                     PTPTPTPTPTPTPTPTPTPTPTPTPTPVAAPIATPAIAVPKAVSVAKPAVGAVTPVPR
                     PVVAKPAAAVPVAKPAVVKVPSAVPVAAKPAPSPAAARAEIELPPDELVGQQIGNYRI
                     DAKIGEGRQGGIYRAFQQNMGRLVRFYALDRVRAADPEEVSRFLSNASVKANVSHAYI
                     LAVYEAGEGNGVYFYSCEYLPCSSLRQLIESGQTLAPRAALQGMKAVCEVLAYFTRDK
                     IAHEMLSANAVLFTANHQPRLANTAAHSVSTPYETADEMREVGRMFLEALEPASPAPV
                     AGLPELLVQLQTPENPYKTWALVAQALAAITPKAAPADAHKLDAQERASIMAIEEAKK
                     RQRRSILLSTAVSLVLVAAALVLVYWLLWGRRTHVKDFSQMVRIPAGEFVYQDGKTET
                     LPEYWID"
     gene            complement(1719..2765)
                     /locus_tag="VIS74_07245"
     CDS             complement(1719..2765)
                     /locus_tag="VIS74_07245"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY5793076.1"
                     /translation="MRFKLPAAATPRSLTLTLSLWLLGALAVVQIFALTWKVIPPIGG
                     RTLAGAMAGKPPEAPYRPEAVAPAAPLPSLPAPPIQQQSLGTQRAQALMAESQKLQNI
                     GQWDQALPFIEQALQILPDDPQILYQYAAVLGRLQRETEALDVLDQLLALRTLDPAKR
                     KEVQKLASFLEQTQYNVQTINPPALAEDGPVNLDIHQKQAGGPLLEDVGLQPGATMGI
                     VDVKEIDAGAGKKGLRIAIKTRPNTKVAAPSVNVLVKFFEKNANGDVVLTSSQAKYEW
                     ISQPIDWKDDLPEILDVIYPLPGSRDASPEDAGNAYYGYVVGVYFNGGLEDNRANPGN
                     LQFEFPFELFLTNK"
     gene            complement(2827..3933)
                     /locus_tag="VIS74_07250"
     CDS             complement(2827..3933)
                     /locus_tag="VIS74_07250"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEY5793077.1"
                     /translation="MKSILRFLIFIVVAALAVSTLYFWRLAQDGEPGPPPLPADPPAP
                     AGSRLEGLAALDEEFVRLVSDAKQSVVSITARQQVQVALRTGNAFQPFRSLGQLQLQP
                     NLGSGVVVSRQGHIITNVHVIRGANEIDARLNDGRIYPATVLGVDPATDLAVLKIDAD
                     NLLPMPLANSDLVRSGQMVFAVGNPYGLHETVTQGIISGIGRTSSEAANEFFQTDTAV
                     NPGNSGGPLINLRGEVIGINNAIHTNTGAWQGISFAIPSNTVRRVFDDIRSYGRVIRT
                     WLGVESDLPRTVRGAAMPGVRIQSVLEGSPAQKSGLLPEDVIVEFNGKPVNGLIDLRN
                     RVLETEAGQQVGARVLRDGFPVNLNLIMEEYPRS"
     gene            complement(4047..>5103)
                     /locus_tag="VIS74_07255"
     CDS             complement(4047..>5103)
                     /locus_tag="VIS74_07255"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY5793078.1"
                     /translation="AEAAQKYSDFLSRFPGHAYVSQVMLERGLAHKAARAYPAALQDF
                     DAIIGKTPPTKEREVALLQKALLLGEQKDYGEMAGAFAQLIQDYPQTAAAAQANFWIG
                     WAAYESKDYPAALPALKKARELDAKNYGDRAGLRIILAYYYLEDADEVAKQIAEHPVT
                     NLPGEVYQWLAAKYLEAKAYAKAEKFLELLASGTLGPVSAETHLSLAQARLEQKKYAG
                     AVEAAQKFLDSARDPLNRARGLMLQAEAELGQSHYDAAAKRADECLLLQPEGAMNAQA
                     RLLSGRILAARGDNDEAARAFMTIAVLYDDPQITPDALKRAAAAYQKAGNALEAQNAL
                     KELEKRYPDQLQKKDFE"
BASE COUNT          868 a         1615 c         1669 g          951 t
ORIGIN      
        1 atcgatccaa tactcgggca gcgtttccgt tttgccatcc tgatacacaa actccccggc
       61 ggggatgcgg accatctggc tgaaatcctt cacgtgggtc cgccgccccc acagcagcca
      121 ataaaccagc acgagcgcgg cggccacgag caccagcgac accgcggtgc tcagcaggat
      181 cgaacggcgc tggcgttttt tggcttcctc gatggccatg atggaagccc gctcctgcgc
      241 gtcgagcttg tgggcatccg cgggcgccgc cttgggggtg atcgcggcaa gcgcctgcgc
      301 caccagcgcc caagtcttgt aggggttttc cggcgtttgc agctggacga gcaactcggg
      361 cagccccgcc accggcgccg gggaggccgg ttccagcgcc tccaggaaca tgcggccgac
      421 ttcgcgcatc tcgtccgccg tttcataggg agtggaaacg gaatgcgcgg cggtattggc
      481 caaacggggc tggtgattgg ccgtgaagag caccgcgttg gcgctcagca tctcgtgcgc
      541 gattttgtcg cgggtaaaat aggcgagcac ttcgcagacc gccttcatgc cctgcagcgc
      601 cgcccgcggg gccagggtct ggccggactc gatgagctgg cgcaatgagg aacacggcag
      661 gtattcgcag gaataaaaat aaaccccgtt tccttcgccc gcctcgtaaa cggccaggat
      721 gtaggcgtgc gacacgttgg ccttgacgct ggcattggaa aggaaccggg acacctcctc
      781 gggatccgcc gcccggacgc ggtccagcgc gtaaaaccgc accaaccgcc ccatgttctg
      841 ctgaaaggcc cggtaaatgc ctccctgccg gccttcgccg atcttggcat cgatccggta
      901 attgccgatc tgctgcccaa cgagctcgtc cggcggcagc tcgatttcgg cgcgggcggc
      961 ggccggggac ggcgcgggtt tggcggcgac gggaacggcc gagggaacct tcaccacggc
     1021 cggtttggcc accgggacgg cggccgccgg tttcgccacc acgggacggg gcacgggagt
     1081 cacggcaccg acggccggct tggccaccga caccgctttg ggcacggcaa tggccggagt
     1141 cgctatcgga gccgccaccg gcgtgggcgt gggcgtgggc gtgggcgtgg gcgtgggcgt
     1201 gggcgtgggc gtgggcgtgg gcgtgggcgt gggcgtgggc gtggcgcgcg gagtcggagt
     1261 cggaacgact tgggggacaa cggccacggg cgtggcaacc gcctcgggaa cggccacggg
     1321 ttgcgcgacg gcgactggag ccgccgccac cgccggccgg gggacgggag ccccgatcat
     1381 ttgccgcacc accgccacca gcgttcccgg atcgagcggc ttgggcagaa acaacgcgcc
     1441 ggccagccgg tcgtcgtagt cggtgagatc ataacccgag atgaaaatcg tgcgcagggc
     1501 ggggagctgc gattgcagga cctcgcggag cgcaaagcca tcctccggat tgaggatgac
     1561 gtcggtgatg agcagatcca gcttattcca tgccgtcacc tgagcgtagg cttcatcaat
     1621 ggaaaccacc gccttcactt cggcgtaacc cgcttgctca aggatcgccg ccagcccgga
     1681 cgcgaggccc ggttcgtcat ccaccaccag aatcttcatt acttattcgt caaaaataac
     1741 tcgaagggaa actcgaattg caggttgccg gggttcgcgc ggttgtcctc cagtccgccg
     1801 ttgaaataaa cgcccaccac atagccataa taggcattcc cggcatcctc cgggctcgca
     1861 tcccgggaac ccggcagggg gtaaatcaca tccaggattt ccggcagatc gtccttccag
     1921 tcgatcggct ggctgatcca ttcatacttg gcctgcgaac tcgttagaac gacatcgcca
     1981 ttcgcattct tctcaaagaa tttcaccagc acgttgacgg acggcgcggc caccttggtg
     2041 ttcgggcggg ttttgatggc gatgcgaagc cccttcttgc cggcgcccgc gtcgatttcc
     2101 ttcacgtcca cgattcccat ggtcgcgccg ggctgcagtc ccacgtcttc gagcagcggc
     2161 ccgcccgcct gcttttggtg aatgtcgaga ttcaccggtc cgtcctccgc cagcgccggc
     2221 ggattgatcg tctggacgtt gtattgggtt tgttccagga aactggccag cttctgcact
     2281 tccttccgtt tggccggatc gagggttcgc aaagccagca attgatccag cacgtccagc
     2341 gcttcggtct cccgctgcag ccggccgagc accgcggcgt attgatacaa aatttgcggg
     2401 tcgtccggca ggatttgcag cgcttgttcg atgaatggca gggcctgatc ccactgcccg
     2461 atgttttgca gcttttgcga ctccgccatg agcgcctggg cgcgctgcgt gccgagggat
     2521 tgttgttgaa tgggcggcgc cggcagactc ggcagcgggg cggcgggcgc caccgcttcc
     2581 gggcgatacg gggcttcggg cggtttgccc gccatcgctc cggccagcgt gcggccgccg
     2641 atggggggaa tcaccttcca ggtcagggca aaaatttgaa caacggccaa ggctcccagc
     2701 aaccaaaggc tgagggtcag agtgaggctg cggggggtgg ccgctgcggg cagtttgaat
     2761 ctcacaaatt gtcaggctta aaaggttggc atgcccctgt caatgcggct cgcgggcgga
     2821 gccccattaa ctgcggggat attcctccat gatcaggttc agattcacgg gaaaaccgtc
     2881 gcgcagcacc cgggcgccga cttgctggcc cgcctcggtt tcgagcaccc ggttccgcag
     2941 atcgatgagg ccattcacgg gcttgccgtt gaactccacg atcacatcct ccggcaaaag
     3001 cccggacttc tgcgccggcg agccttccag cacggactgg atgcgcacgc cgggcatcgc
     3061 ggctccgcgc accgttctcg gcagatcgga ctcgaccccg agccaggtgc ggatcacccg
     3121 gccgtaactg cggatgtcgt caaacacgcg gcggaccgta ttcgagggaa tggcaaagct
     3181 gatgccctgc caggcgccgg tgttcgtgtg aatggcgtta ttgatgccga tgacttcgcc
     3241 gcgcagattg atcaacgggc cgcccgagtt gccgggattc accgccgtgt cggtctggaa
     3301 aaattcgttc gccgcctcgc tggaagtccg gccgatgccg ctgatgatgc cctgcgtcac
     3361 ggtctcgtgc aggccgtagg gattgccgac ggcgaacacc atctgccccg agcgcacgag
     3421 gtcggaattg gccagcggca tgggcaggag gttgtcggcg tcgattttca gcaccgcgag
     3481 gtcggtagcc gggtccacgc cgagaaccgt ggcggggtag atgcggccat cgttgagccg
     3541 cgcgtcgatt tcgttcgcgc cgcggatgac gtggacattt gtgatgatgt ggccctgcct
     3601 ggaaaccacc accccggagc ccagattcgg ctggagctga agctggccga gcgagcggaa
     3661 gggctggaag gcatttcccg tgcgcagggc cacctgcacc tgctggcgcg cggtgatgct
     3721 gaccaccgac tgcttggcgt cgctcaccag ccggacaaac tcctcatcca gcgccgccag
     3781 cccctccagg cggcttccgg ccggcgcggg cggatccgcg ggaagcggcg gcggacccgg
     3841 ttcgccgtct tgggcaaggc gccagaaata aagcgtgctg accgcgaggg cggccacgac
     3901 gatgaagatc agaaagcgaa ggatgctttt caagtttggc ggaatttcaa aatggcttca
     3961 ccggccggcg ggcgtggata agtcaaaacg gggcggccgc ccggtcggat ttatccgcgg
     4021 ttttcgccgc ggggttacgc ataaggttac tcgaaatcct ttttctgcaa ctggtccggg
     4081 tatcttttct cgagttcctt gagcgcattc tgcgcttcga gcgcattgcc ggctttctgg
     4141 taagcggcgg ccgcccgctt cagggcgtcg ggggtgattt gggggtcgtc gtataacacg
     4201 gcgatcgtca taaacgcccg ggcggcttcg tcattgtcgc cgcgggcggc gaggatgcgg
     4261 cccgagagca ggcgggcctg ggcattcatc gccccctcgg gttgaaggag caggcattca
     4321 tccgcccgct tcgcggcggc atcgtaatgg ctctgcccga gttccgcctc cgcctgcagc
     4381 attaaccccc gggcgcgatt cagcggatcc cgcgccgaat cgaggaattt ctgcgccgcc
     4441 tccaccgcgc cggcgtattt cttttgctcc agccgggcct gggccaggct caggtgggtc
     4501 tcggcggata ccggccccaa ggttccggat gcgagcagtt cgagaaattt ctccgccttc
     4561 gcatacgctt tcgcttcgag gtatttcgcg gccagccatt gatacacctc gccgggcaga
     4621 ttcgtcaccg gatgctccgc gatctgcttg gccacctcgt cggcatcttc cagataataa
     4681 tacgcaagga tgatccgcag cccagcgcga tccccgtaat ttttggcgtc cagctcgcgc
     4741 gcctttttca gggcgggcaa cgccgccggg taatccttgg actcgtaagc cgcccagccg
     4801 atccagaaat tcgcctgggc cgccgcggcg gtctgcggat agtcctgaat caattgcgca
     4861 aaggctcccg ccatctcgcc gtaatccttc tgctcgccga gcagcagcgc cttttgcaac
     4921 agggccacct cccgttcctt ggtcggcgga gttttgccga tgatggcatc gaaatcctgc
     4981 agggcggcgg gatacgcgcg ggcggccttg tgcgccaggc cccgctcgag catcacctgc
     5041 gagacatagg catggccggg aaagcggctt aagaagtcgc tgtatttctg cgcggcctcg
     5101 gcc
//
LOCUS       DATDAY010000366         2141 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_667584, whole genome shotgun sequence.
ACCESSION   DATDAY010000366 DATDAY010000000
VERSION     DATDAY010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2141)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2141)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2141
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_667584"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..589
                     /locus_tag="VIS74_07260"
     CDS             <1..589
                     /locus_tag="VIS74_07260"
                     /inference="COORDINATES: protein motif:HMM:NF025019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4143 domain-containing protein"
                     /protein_id="HEY5793079.1"
                     /translation="LMNLSAAATGSLLNQAHLARDAGMSPATAGRHFSILEASFLITR
                     VPPFFANIGKRMVKAPKLYWNDVGLASHLCGISSVEALRGSLMLGRLVETLIMMEIQA
                     LLPLAEEPARLFHVRTHDGLEVDGLLEIGQRHLPLEIKASQTVTASDAAPIERWIALN
                     PKHGPGIVIYLGEEYLTLSANVRAVPAGALFGSPK"
     gene            656..1108
                     /locus_tag="VIS74_07265"
     CDS             656..1108
                     /locus_tag="VIS74_07265"
                     /inference="COORDINATES: protein motif:HMM:NF039513.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Wadjet anti-phage system protein JetA family
                     protein"
                     /protein_id="HEY5793080.1"
                     /translation="MALSAELQTQLREGFFRPLGRPSWPVYVDAGDRLLGSSSEGGQL
                     TREDALALLRDVLMEHPSAQLDADEGAGFSDARRRATQLYNRRLEAGWLQERPGSLGE
                     YWVLVTPALRLLVRLLRDLARSDLGELKDFAASLRSICEALPARRLRI"
     gene            1120..1974
                     /locus_tag="VIS74_07270"
     CDS             1120..1974
                     /locus_tag="VIS74_07270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HEY5793081.1"
                     /translation="MNPIFRQSAASCFVPDGSAPEAALARCTHLGVAAHPDDLEFMAC
                     HGILECHLSESKGFGGVICTDGAGSSRMGPFADFTDEEMRAVRRREQEAAAVIGCYRA
                     LVQLAYASREVNRPGQTPLVDDLKGILELARPRVVYTHNPADKHETHIGVMAATLAAI
                     RSLPPEQRPGAVWGCEVWRDLDWMPDEEKVALDVSARPDLAEALNRVFESQIAGGKRY
                     DLAVMGRRRANATFFASHAADQAQMLTFAMDLTPLAREESLDVTAYVTGLIDRFREDV
                     AQKLKRYF"
BASE COUNT          384 a          622 c          706 g          429 t
ORIGIN      
        1 cctcatgaac ctcagcgcgg cggccacggg cagcctgctc aatcaggcgc atctggcacg
       61 cgatgccggc atgagtccgg caacagctgg acggcatttt tccattttgg aggcgagttt
      121 cttaatcaca cgggtgccgc cttttttcgc caatatcgga aaacggatgg tcaaggctcc
      181 gaaactctac tggaacgatg tgggcttggc ctcgcacctt tgcggcattt cgtccgttga
      241 ggccctgcgg ggtagtttga tgctcgggcg cttggtggaa acgcttatca tgatggaaat
      301 tcaggcattg cttccgctgg ccgaggaacc ggcccggctt ttccatgtcc gcacccacga
      361 tggtttggaa gtggatggcc tcttggaaat tggtcagcga catttgccgc tggaaatcaa
      421 agcctcccag accgtcacgg cttccgatgc cgcccccatc gaacgctgga tcgctctcaa
      481 tcccaaacac ggtcccggca tcgtgatcta tctcggcgag gaatatctga ccctttccgc
      541 aaacgtgagg gccgttccgg cgggggcgct gtttggatcc cccaagtaaa tacgtgagca
      601 attcatgcga tgttgagatt gattggctga cgaagaaatt taggatcgac gtccgatggc
      661 actgagcgcc gaactccaga cgcagcttcg cgagggcttt ttccgtccgc tcggacggcc
      721 gtcgtggccg gtgtatgtgg acgcgggcga ccggctttta gggagttcca gcgaaggcgg
      781 gcaactcacc cgggaagacg cgttggccct cctgcgggat gtgctgatgg aacacccttc
      841 ggcgcaactc gacgcggatg agggggccgg attttcggac gcgaggcggc gcgcgactca
      901 gctctacaat cgcaggttgg aggcgggctg gctccaggaa cgtccgggtt ctctgggcga
      961 atactgggtg ctcgtcactc cggcgctgcg gctgctcgtc cggttgctgc gggatttggc
     1021 gcggagcgat ctgggcgaac tgaaagattt tgcggcgtcg ttgcgctcga tctgcgaggc
     1081 gttgccggcc cggcggttaa ggatttaagt cacatcgcga tgaaccccat ctttcgccag
     1141 tctgccgcca gttgtttcgt cccggatggc tccgctcccg aggcggcgct ggcccggtgc
     1201 acgcatctgg gcgtcgcggc gcatccggac gatctggagt tcatggcctg ccatggcatt
     1261 ttggagtgcc atctttccga atcgaaggga tttggcggcg tgatctgcac cgacggcgcg
     1321 ggcagttcgc gcatggggcc ttttgcggac tttaccgatg aagagatgcg cgcggtccgc
     1381 cggcgggagc aggaagccgc ggcggtcatc ggctgctacc gggcactggt gcaattggcc
     1441 tatgccagcc gggaagtgaa tcggcccggc cagacgccgc tggtggacga tttgaaaggg
     1501 attctggagc tcgcccggcc ccgggtggtt tacacgcaca atcccgcgga caagcacgag
     1561 acccatatcg gggtcatggc ggccacgctg gccgccatcc gctcgctgcc gccggagcag
     1621 cggcccgggg cggtctgggg ctgcgaagtc tggcgggatc tggactggat gccggatgag
     1681 gaaaaggtgg ccttggacgt cagcgcccgc cccgatctgg cggaggcgct caaccgggta
     1741 tttgagtccc agatcgccgg aggcaagcgc tacgacctgg ccgtgatggg ccgccgccgc
     1801 gccaatgcca ccttctttgc ctcgcacgcc gccgatcagg cgcagatgct cacgtttgca
     1861 atggacctga cgcccttggc gcgggaggaa agtctggatg tgaccgccta tgtgaccggg
     1921 ctgatcgacc gattccggga agacgtcgcc caaaagctca agcgctattt ttaacccggc
     1981 gaattccccg caacgggctg gcgaatgcca aagcccatgc tggatgtgct gattgtgaga
     2041 tcgagacacc gcgcggggtg gtcaagaaaa tgccagggtt cgcgcgcatc gttgacatcc
     2101 tggaatgttc gtaaaaccta tgaagcaatg aaaacggcga g
//
LOCUS       DATDAY010000367         2237 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_2434972, whole genome shotgun sequence.
ACCESSION   DATDAY010000367 DATDAY010000000
VERSION     DATDAY010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2237)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2237)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2237
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2434972"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..250)
                     /locus_tag="VIS74_07275"
     CDS             complement(<1..250)
                     /locus_tag="VIS74_07275"
                     /inference="COORDINATES: protein motif:HMM:NF039484.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5793082.1"
                     /translation="MKSLWPWLCALLTGVLLALGFVPFDQGGLIWVALTPLVAAVWFG
                     PRWTRREWLRGPLLGYLAGLVYFLVSFFWLSTVTRAGWA"
     gene            complement(247..990)
                     /locus_tag="VIS74_07280"
     CDS             complement(247..990)
                     /locus_tag="VIS74_07280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5793083.1"
                     /translation="MKTTLPKLKWLLTTVLLAIAGVLLARNWPPPASPPAENGIWMAH
                     SPAAPAAKSSPALEAEFLAAKADAVSLQQRLEAAKHAIEEALQMVQEQRDNFDFAEER
                     YHRLMPLVEKGTLDLMTASQIQSAYIAARASLSQAKFYLGQAQREYGTPETRTILLQA
                     AGGKVEAIRQRMGVAGGSPQGGMIVAEFPAALAQTLAPGASVKVIGKSGEFSAVVRQA
                     NGGLVLLDAPGAPAGLPALARCQVVIPAQ"
     gene            complement(990..2000)
                     /locus_tag="VIS74_07285"
     CDS             complement(990..2000)
                     /locus_tag="VIS74_07285"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEY5793084.1"
                     /translation="MRIWILGVSLALAAPGMGQWLVPPTENQALFNGPPSQFYMHVER
                     NFEGQLTKPWEGGQYGYVRGPHRVGSCVLYGHLHEGIDIAPLHRDAAGEPLDPVAAAA
                     AGRVAYVSDQPGASNYGRYVVLEHRLDGGVYYTLYAHLKRVRVQPGEPVAQCQPIGDM
                     GYTGDGITRERSHLHFEVCLLASGKFQEWYDLYQKSSPNKHGLINGMNLIGMDPAALL
                     RGVRANPRLTIPDFVRSLKPLFQITINDSPNFELIRRYPWLVGNGEIANPPAWTITFS
                     ETGVPMRVRAYSRPIGEPLVEWLDPAIPPTLQSRGWIGGTASAPRLTDSGGRFLHLLA
                     FD"
     gene            complement(1997..>2237)
                     /locus_tag="VIS74_07290"
     CDS             complement(1997..>2237)
                     /locus_tag="VIS74_07290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5793085.1"
                     /translation="LLHAATTQHRNSYMIPVINGRPVSLLFISKPVADGVLTVPSGLT
                     AAEISLIARDFPIYGETSSDTKNRQAAEKKKRKPQ"
BASE COUNT          416 a          706 c          755 g          360 t
ORIGIN      
        1 cggcccagcc cgcccgcgtc accgtcgaaa gccagaagaa gctgacgaga aaataaacca
       61 ggcccgccag ataaccgagc agcgggccgc ggagccattc gcgccgcgtc cagcgcgggc
      121 caaaccacac ggcggcgacg agcggcgtca gcgccaccca gatcaaaccg ccctgatcaa
      181 acgggacgaa acccagggcg agcaaaacgc cggtcagaag cgcgcagagc cagggccaga
      241 gggatttcat tgggcgggaa tcaccacctg gcagcgggcg agggcgggaa ggccggcggg
      301 agcgccgggc gcatcgagca gcacgaggcc gccattcgcc tgccggacca ccgcggagaa
      361 ctcgccggat ttcccgatca ctttcacgga agcgcccggc gcaagcgttt gcgcgagggc
      421 cgcgggaaat tccgccacga tcatgccgcc ttgcggcgaa ccgcccgcca cccccatgcg
      481 ctggcggatg gcttccacct tgcccccggc ggcctgcagg agaatggtcc gggtttccgg
      541 cgtgccgtat tcccgctggg cctgcccgag ataaaatttc gcctgcgaga gcgacgcgcg
      601 ggcggcgatg taggcgctct gaatctgcga ggccgtcatc aagtccagcg tgcctttttc
      661 gaccaggggc atgagccggt gataccgctc ctccgcgaag tcgaagttgt cgcgctgctc
      721 ctggaccatc tgaagcgctt cctcgatcgc gtgtttggcg gcctcgaggc gctgctgcaa
      781 cgagaccgcg tccgccttcg cggcaaggaa ttccgcctcc agcgcgggcg aacttttcgc
      841 ggccggagcc gccggggaat gggccatcca aatgccgttc tccgccggcg gggaagcggg
      901 cggaggccag ttgcgggcga gcaaaacccc ggcaatggcc agcagcaccg tggtcagcag
      961 ccatttgagt ttcgggagcg tggttttcat caatcaaagg ccagcaggtg aagaaaccgg
     1021 ccgccggaat ccgtcaaccg gggcgcggac gcggttccgc cgatccagcc gcgggactgc
     1081 agcgtgggcg gaatcgcggg atcgagccat tccaccagcg gctcgccgat cggacgggaa
     1141 taagccctca cgcgcatcgg cacgccggtt tcgctgaagg tgatcgtcca ggcgggcggg
     1201 ttggcgattt cgccattgcc caccagccag ggataacggc ggatgagttc gaagttcggt
     1261 gaatcattga tcgtgatctg aaagagcggc ttcaacgaac ggacaaagtc gggaatggtc
     1321 aaccgcggat ttgcgcgcac gcccctgagc agggcggccg gatccatgcc gatcaggttc
     1381 atgccattga tgaggccgtg cttgttgggc gaactttttt ggtaaaggtc gtaccattcc
     1441 tgaaatttgc cgctcgccag caggcagacc tcgaagtgca agtgggagcg ttcgcgggtg
     1501 atcccgtcgc cggtgtagcc catgtcgccg atcggctggc actgggccac cggctcgccg
     1561 ggctggacgc gcacgcgctt gaggtgcgcg taaagggtgt aatagacgcc gccgtccagc
     1621 cgatgttcca gcaccacgta acgcccataa tttgacgcgc ccggctggtc ggacacatac
     1681 gcgacccgtc ccgccgcggc ggcggccacc gggtccagcg gctcgcccgc cgcgtcccgg
     1741 tggagcgggg cgatgtcaat gccctcgtgc aaatggccgt aaagcacgca ggagccgacc
     1801 cggtgcggac cgcgcacgta gccgtattgc ccgccttccc agggtttggt gagctgcccc
     1861 tcgaagttgc gctccacgtg catgtaaaat tgggacgggg gaccattgaa taatgcctgg
     1921 ttctccgtgg gagggaccag ccattgcccc atccccggcg cggccagggc gaggctcacg
     1981 cccagaatcc agatcctcat tgcggcttcc gctttttttt ctcggcggcc tggcggtttt
     2041 tcgtatcgct ggaggtttcg ccatagatcg gaaagtcccg cgcgatgagg gaaatctccg
     2101 ccgcggtgag gccggagggg acggtcagaa cgccatccgc caccggtttg ctgatgaaga
     2161 gcaacgaaac ggggcggcca ttgatgacgg gaatcatgta ggaattccga tgctgggtgg
     2221 tggcggcatg gagcagg
//
LOCUS       DATDAY010000368         2292 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_239498, whole genome shotgun sequence.
ACCESSION   DATDAY010000368 DATDAY010000000
VERSION     DATDAY010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2292)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2292)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2292
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_239498"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..217)
                     /locus_tag="VIS74_07295"
     CDS             complement(<1..217)
                     /locus_tag="VIS74_07295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5793086.1"
                     /translation="MNPFSEISFENRSKSDEGKISADFTDFLRRGSRILKNLRRNARE
                     AAARLSNRWIKSAFPQRTIPRWLARMKS"
     gene            complement(567..>2292)
                     /locus_tag="VIS74_07300"
     CDS             complement(567..>2292)
                     /locus_tag="VIS74_07300"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HEY5793087.1"
                     /translation="FYQRWAAERPRSITAQVCLIQALTSYAWRARGSGWADSVTEEGW
                     RLFGERLDQAADVASRAKELPEKCPGLVHAAQKVALGQGWDRAEYFDMVDPAIAAEPT
                     FGSYYTSACYYLFPRWYGEPGDFEKWIAAQAAAYPPAERDRQYARLVWLADRNSMPEE
                     MVFAPGRLDWERARRGFAEWLQAEPDSLIVRMEFTRLAVLADDRETARAQFDLTGGQY
                     FWGVWRKESRFEGARQFAYAEGPNPFREESDEPPVHFDEKTRQWVQLGLRLINGFCGG
                     FLAGGLLLLLAIQRKRPWAGVGAGLLSLLLGTAFGTLATTLAGAALWFYLRRQRLEHP
                     PSVGGPPAWLTLLWTVLLALVYLGLQIAATIPAVIPLFLESGEMNETGLLRQLMLSGA
                     PFHIFLSASWITFLLLLAVCGPRNAAGWRDRLGLHRCALLPSMGWTLAAGALLIGGGL
                     LLEPYLDARTREAMELLKFGMNTPWLLFASIVLVAPVVEELIFRGYAYSGWMAKLGFW
                     GAALLSSLLFMACHLQYGWSGLLEIFFLGILLAAVRWKTGSVYPCIALHVLNNLAGYL
                     HFLPEAGS"
BASE COUNT          469 a          737 c          722 g          364 t
ORIGIN      
        1 tggatttcat ccgcgccagc cagcggggaa tcgtgcgctg aggaaacgct gatttaatcc
       61 accgatttga aagcctggcc gccgcttcgc gtgcgttgcg gcgcagattt tttaaaatcc
      121 gcgatcctcg ccgcaagaaa tcggtgaaat cggcggatat tttcccttca tctgatttag
      181 agcgattctc aaaagagatt tccgaaaagg gattcaatgt aggggcagcc ggagcgaaga
      241 cagcctgtcc tgcggaagcg ggacagcccg aggggcgcac gaagtgcggc aattgtgcca
      301 accgcccagc gtaaacggga tgcggggtcc ccgtttcccc gcaaacaaag gcgcccaatg
      361 gggaagtggg ggccccattt ccccacccga caaccgcctc acaacccgtt gaggtatttc
      421 agaaatctct tttgagaaat actataatca gcggttcctt aacggattca gttcgtcaag
      481 tgagcggcgg cgttggcaac gccgccccac cggggggagc ggtttatggg gttatggcgg
      541 ggttgccaac cccgccgggg aaagcgctaa cttcccgcct cggggagaaa gtgcagatag
      601 ccggccaggt tattgaggac gtgcagcgcg atgcacggat atacgctgcc ggttttccag
      661 cggacggcgg cgagcaggat tcccagaaag aaaatttcca gcaggccgct ccagccgtat
      721 tgcagatggc aggccatgaa gagcagggag ctgagcagcg cggcgcccca gaaccccagc
      781 tttgccatcc agccggaata ggcgtaaccc cggaaaatga gttcctcaac caccggcgca
      841 accaggacga tgctggcaaa gagcagccac ggcgtgttca ttccgaattt gaggagttcc
      901 atcgcttccc gcgtgcgggc gtcgagatac ggctccagga ggaggcctcc gccgatcaac
      961 agcgccccgg cggccagggt ccaccccatc gacgggagca gcgcgcagcg atgaagcccc
     1021 agccggtccc gccaccccgc cgcgttccgc ggcccgcaca cggcgagcag gagaagaaaa
     1081 gtgatccagc tggcgctgag aaaaatatgg aagggagcgc cgctgagcat taattggcgc
     1141 agcagcccgg tttcattcat ctccccggat tcgagaaaga ggggaatgac cgccggaatc
     1201 gtcgcggcga tttgcaaccc gagataaacc agcgccagca ggacggtcca gagcagcgtc
     1261 agccaagcgg gcggcccgcc gacggagggc ggatgttcga gccgctggcg gcggaggtaa
     1321 aaccagagcg ccgcgcccgc gagcgtggtg gccagggtgc caaaggccgt gccgaggagg
     1381 agactcagca ggccggcgcc cacgccggcc caggggcgtt tgcgctgaat cgccaggagg
     1441 agcagcagcc cgccggccag aaagccgccg cagaatccgt tgatcaacct taggccgagc
     1501 tggacccatt gccgggtttt ttcgtcgaaa tgcaccggcg gctcgtccga ttcctcgcga
     1561 aagggattgg gcccctccgc ataggcgaac tgccgcgcgc cctcaaagcg ggattccttc
     1621 cgccagacgc cccagaaata ttggccgccg gtgaggtcga actgcgcccg cgccgtctcg
     1681 cgatcatcgg ccagcacggc gaggcgggtg aattccattc gcacgatcag gctgtccggc
     1741 tcggcctgca gccactcggc aaacccccgc cgcgcgcgct cccagtcgag ccggccgggc
     1801 gcgaacacca tttcctcggg catggaattg cgatccgcca gccacaccag gcgggcgtat
     1861 tgccggtcgc gctccgccgg cggataggcc gccgcctgcg ccgcgatcca tttttcgaaa
     1921 tcccccggct cgccatacca gcgcgggaac aaataataac acgccgacgt gtaataactt
     1981 ccgaaagtcg gctcggcggc gattgcggga tccaccatgt cgaaatactc cgcgcggtcc
     2041 cagccctggc cgagcgccac tttttgggcg gcatgcacca ggccggggca tttttccggc
     2101 aattctttgg cgcggctcgc cacatcggcg gcctggtcca ggcgttcgcc aaacaagcgc
     2161 cagccttcct cggtgacgct gtccgcccag ccgctgccgc gcgcccgcca cgcataggat
     2221 gtcagggcct gaatgaggca aacctgcgcc gtgatgctcc gcggccgctc tgccgcccag
     2281 cgttggtaaa ac
//
LOCUS       DATDAY010000369         2215 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_2437957, whole genome shotgun sequence.
ACCESSION   DATDAY010000369 DATDAY010000000
VERSION     DATDAY010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2215
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2437957"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..479
                     /locus_tag="VIS74_07305"
     CDS             <1..479
                     /locus_tag="VIS74_07305"
                     /inference="COORDINATES: protein motif:HMM:NF028108.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5069 domain-containing protein"
                     /protein_id="HEY5793088.1"
                     /translation="DPEPYTGNVEPSLSLPELRGPYETVGGIVFFGRMLDKIRIFQAG
                     YLPDAWANAMGASRNFDSILCRFLGLDYSHFYAEALKGGVNDEDMLQWTFAHGRQPSE
                     EEILIFNSYLSKRNWRDAYTPRLHFRLRESGMPLNAARTMFDYIDLDEGRPRRFSW"
     gene            complement(483..1424)
                     /locus_tag="VIS74_07310"
     CDS             complement(483..1424)
                     /locus_tag="VIS74_07310"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HEY5793089.1"
                     /translation="MQLHGIHHLTAISADASGNHAFYTRGLGLRLVKKTVNQDDPSAY
                     HLFYADGLASPGSDLTFFDWPMPRERRGTRSILRTGLRVGSAESLEWWRRRFEDLKIA
                     HGAIQERDGRETLDFEDPEGQRLSLVDDGGIGGAHPWAKSPVPAKHQIRGLGPITLSV
                     PRLSRTDQVLREVLAMRPVRDYFIGEKTAVRVYQMDGAGPAAELHVAEEPEKETAHQG
                     AGGVHHVAFRTTTADYAAWVSRLNDFGVASSGPVDRYYFRSLYFREPNGILFEIATDE
                     PGFTADEPLETLGERLSLPPFLEARRAQIEAGLKPLG"
     gene            complement(1451..1936)
                     /locus_tag="VIS74_07315"
     CDS             complement(1451..1936)
                     /locus_tag="VIS74_07315"
                     /inference="COORDINATES: protein motif:HMM:NF012478.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEY5793090.1"
                     /translation="MQITIGKVVSIDYTLTDDRQQTLDSSAGGEPLVYLHGVGQLIAG
                     LENELEGKRAGETLKVKVPPAEGYGLRDESKVGVIPRAQIQGAGNLRVGAQLQASSPD
                     GSQILTITKVEDDQITVDANHPLAGENLHFDVTIRDVRDATPEEASHGHVHGPGGHHH
                     H"
BASE COUNT          359 a          713 c          721 g          422 t
ORIGIN      
        1 cggatccgga gccttatacc ggcaacgtgg agccatccct ctccctgccg gagctgcgcg
       61 ggccctacga aaccgtgggc ggcatcgttt tcttcggacg aatgctcgac aaaatccgca
      121 tcttccaggc cggttacctg ccggatgcct gggcgaatgc gatgggcgcc tcccgcaatt
      181 ttgattccat tctctgccgc tttctcgggc tggactattc ccatttttac gccgaggcgc
      241 tcaagggcgg cgtcaacgac gaggacatgc tgcaatggac cttcgcccac ggccgccagc
      301 ccagcgagga ggaaattctc attttcaatt cctacctcag caagcgcaac tggcgggacg
      361 cttacacgcc gcgcctgcat ttccgcctgc gggaaagcgg catgccgctg aatgccgcgc
      421 ggaccatgtt cgattacatc gacctcgacg aaggccgccc gcggcggttc tcgtggtagg
      481 gcctacccca gcggcttgag tccggcttcg atctgcgccc ggcgcgcttc gaggaagggc
      541 ggcagggaca ggcgttcccc gagcgtttcc agcggttcgt ccgcggtgaa gcccggctcg
      601 tccgtggcga tctcgaacag aatgccgttc ggctcccgaa aatacaggct gcggaaataa
      661 taacgatcca ccggaccgct ggaggcgacg ccgaaatcgt tcaagcggct cacccacgcc
      721 gcgtaatccg ccgtggtcgt gcggaaggcc acgtgatgca ccccgcccgc gccctggtgc
      781 gcggtttcct tttccggctc ctccgcgacg tgcaattcgg ccgcggggcc ggcgccgtcc
      841 atttggtaaa ccctcacggc ggttttttcc ccgatgaaat aatcccggac cggccgcatc
      901 gccaggacct cgcgcagcac ctggtccgtg cgggacaggc gcggaacgct cagcgtgatg
      961 ggaccgagcc cgcggatttg atgtttggcg ggcaccgggc ttttcgccca cggatgggcg
     1021 ccgccgatgc cgccatcatc gaccaaactc aggcgctggc cttcggggtc ctcgaaatcg
     1081 agggtctccc gcccatcgcg ctcctggatc gctccgtggg caatcttcaa gtcctcaaag
     1141 cggcggcgcc accattccag cgattccgcg ctgccgacgc gcaggccggt gcggagaatg
     1201 ctgcgcgtcc cccgccgctc ccgcggcatc ggccagtcga agaaagtcag atcgctgccc
     1261 ggcgacgcca gcccgtccgc gtagaataaa tgatacgcgg acgggtcgtc ctgattgacc
     1321 gtttttttca ccagccgcaa gccgaggccg cgggtataga aggcgtgatt tccggaggcg
     1381 tccgcggaaa tcgccgtcag gtgatggatg ccgtgcagtt gcatgggacg ctccccggag
     1441 gttccgccga tcaatgatgg tgatgcccgc cgggcccgtg cacgtggccg tgggaggctt
     1501 cctccggcgt ggcgtcccgc acgtcgcgaa tcgtcacgtc gaaatggagg ttttcgcccg
     1561 ccagcggatg gttggcgtcc accgtgatct ggtcgtcttc gactttggtg atggtgagaa
     1621 tctgcgagcc gtccgggctg ctggcctgca attgcgcgcc gacccggaga ttgccggccc
     1681 cctggatctg ggcgcgggga atgacgccga ccttggattc gtcgcgcagg ccgtagccct
     1741 cggcgggagg caccttgacc ttgagggttt ccccggcccg tttgccttcg agctcatttt
     1801 caaggcccgc gattaattgc cccacgccgt ggagataaac cagcggctcg ccgccggcgg
     1861 aggaatcaag ggtttgctgg cggtcgtcgg tgagcgtgta atcaatgctg acgacctttc
     1921 cgatggtgat ttgcataatg ggatcacgtt aagggtgcga acggcgattg cactccggac
     1981 ttttggggca gtcgtctgac ggcgagggaa gttgatttca agcgggggag cgtgcaggtt
     2041 gaaggcgatg aaacgcctgc ttctcctgct ggccctgatc gggtgcctga cccccctgcg
     2101 cgcccaggtg ctcgacggcg tggaactcgt ccaggcgcgg ctgatcgccg atgtggacgc
     2161 ggcggcgccg gggcagactt ttaccgccgg cgtggagctc cggatggcgc cgaag
//
LOCUS       DATDAY010000370         2264 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_1555971, whole genome shotgun sequence.
ACCESSION   DATDAY010000370 DATDAY010000000
VERSION     DATDAY010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2264
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1555971"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..519
                     /locus_tag="VIS74_07320"
     CDS             <1..519
                     /locus_tag="VIS74_07320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5793091.1"
                     /translation="QLESETTTIRAADCETRLMADGALLGQIALQLEHNGAVSWHFRL
                     PEESQLLSCEVRGRPANPIIREGGQLELEIPAGGERTSAIKLTYTSRQPKFDPLRGRL
                     ALALPVTPLFIYKLNWRIALPDPYLVDAFEGNLAIPKGPVPPGVLQLEKQLIRGEQPQ
                     AEIFYIKNNPEK"
     gene            527..1744
                     /locus_tag="VIS74_07325"
     CDS             527..1744
                     /locus_tag="VIS74_07325"
                     /inference="COORDINATES: protein motif:HMM:NF017893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane CreD family protein"
                     /protein_id="HEY5793092.1"
                     /translation="MKSQTLTFVRILAIGFILAGAGFAWFVLGGSLQLRTMHSDSELA
                     SQVADGWGPPLQQSQPAFWIASPSANGGKKLIQPESTHIRVRLESDPLKKGLLWYRGY
                     RVRFEGEYQIVNPTPIAQTLYADFLFPSEKNTYFDVEFEFGDQKTGNLDASRGLISGA
                     VNVPANGTVRLRVAYRSRGLNRWDYVLNDSARVRGFNLRMETDFAEINFPEGTGSPTS
                     RSVAPDQGWVLNWSYPDVIGARPIGMDMPKVLNAGPVAARISFFAPVSLVFFFAVLLI
                     LGSVRNINLHPMNYFFLAAGCFAFQLLFAYLVDVLPIHLSFVLSALVSMVLVSGYLHA
                     MGGRALSSISIPAQFAYMVLFSYSFFFDGFSGLTITLGAVATLAILMIATAKVDWSEK
                     FSRKPPVPIPAEA"
     gene            1800..>2264
                     /locus_tag="VIS74_07330"
     CDS             1800..>2264
                     /locus_tag="VIS74_07330"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY5793093.1"
                     /translation="MSPNPSAIKRPRILVAEDEPSIRDAITYPLESDGCEVRACGTGA
                     EILQCMAAAKFDVLILDIGLPDMTGFEVCKKIRERHSLPILFLTARADEIDRVVGLEI
                     GGDDYVTKPFSPRELSARVRALLRRIAREDGAAPAVSSVLVVNEERFVVHYHG"
BASE COUNT          393 a          713 c          703 g          455 t
ORIGIN      
        1 caactcgaat ccgagacgac gacgatccgc gcggcggatt gcgaaacgcg cctgatggcg
       61 gatggcgcgc tgctgggcca gatcgcgctc cagctcgaac acaacggcgc ggtgagctgg
      121 catttccggc tgccggagga aagccagttg ctgagctgcg aagtgcgcgg ccgcccggcg
      181 aatccgatca tccgcgaagg cggccagctc gaactggaaa tccccgccgg cggggaacgg
      241 acttcggcca tcaagctgac ctacacctcc cgccagccga aattcgatcc cctgcgcggc
      301 cgcctggcgc tggctctgcc ggtgacgccg ctgttcattt acaagctcaa ttggcggatt
      361 gcgcttcccg atccctacct cgtggacgcc ttcgagggga atctggccat tccgaaaggc
      421 ccggtcccgc ccggtgtcct gcagctcgaa aaacaattga tccgcggcga gcagccgcag
      481 gcggaaatct tctacatcaa aaacaaccct gaaaaataac cacatcatga aatcccaaac
      541 tctgaccttt gttcgaatcc tggccattgg cttcatcctg gccggtgccg gcttcgcctg
      601 gtttgtcctg ggcggctcgc tccagctccg aacgatgcat agcgattccg aactcgccag
      661 ccaggtggcg gacggctggg ggccgccatt gcagcagagc cagccggctt tctggatcgc
      721 ctcgccctcg gcgaacggcg gcaaaaaact catccaaccg gagtccacgc acatccgcgt
      781 ccggctggaa agcgatccgc tgaagaaggg gctgctgtgg tatcgcggtt accgcgtgcg
      841 gttcgagggc gaataccaga tcgtgaatcc gacgcccatc gcccagacgc tctatgccga
      901 cttcctgttc ccgtcggaga agaacacgta tttcgacgtg gaattcgagt ttggagatca
      961 aaagacgggc aacctggatg cctcgcgggg cctgatttcc ggggcggtga acgttcccgc
     1021 caacggaacg gtgcggctgc gcgtggcgta tcggtcgcgc ggcttgaacc gctgggatta
     1081 tgtgctcaac gacagcgccc gggtgcgggg tttcaacctg cgaatggaaa cggacttcgc
     1141 ggagatcaat ttccccgagg ggacgggctc gccgacctcg cgcagcgtgg cgcctgacca
     1201 gggctgggtg ctgaactggt cgtatccgga cgtgatcggc gcgcggccca tcggcatgga
     1261 catgcccaag gtgcttaatg ccgggccggt cgccgcgcgc atcagcttct tcgccccggt
     1321 gtcgctggtg ttcttctttg ccgtgctgct gatcctcggc agcgtgcgga acatcaacct
     1381 gcatccgatg aattatttct tcctcgccgc cgggtgcttt gccttccaac tgctctttgc
     1441 gtatctcgtg gacgtgctgc cgattcacct gagctttgtt ttatccgccc tggtttccat
     1501 ggtgctcgtc agcggttacc ttcacgcgat gggcggacgc gcattgagtt cgatttcgat
     1561 cccggcgcag ttcgcctaca tggtgttgtt cagctacagc ttcttcttcg atggcttcag
     1621 cggcctgacc atcacgctgg gagcagttgc cacgctggcc attctcatga ttgccaccgc
     1681 gaaggtggat tggtcggaaa agttcagccg caaaccgccg gttcccattc cggccgaggc
     1741 ttgatgaatt gtcggctgga gctggcgggg aagacccgct aggctccggc cgaagccgca
     1801 tgtctcccaa cccttccgca atcaaacgtc cgcgcattct ggtggcggag gatgagccgt
     1861 ccatccgcga tgccatcacg tatccgctgg agtccgacgg ctgcgaggtg cgcgcgtgcg
     1921 ggacgggcgc cgagattctg caatgcatgg ccgccgcgaa atttgacgtg ctgattctcg
     1981 atatcggcct gcccgacatg acgggcttcg aggtctgcaa gaaaatccgc gagcgccatt
     2041 ccctgccgat tctttttctg accgcgcggg cggatgagat tgaccgcgtc gtcggcctgg
     2101 agattggcgg ggatgattac gtgaccaagc cgttcagccc gcgcgaactc agcgcgcgcg
     2161 tccgggcgct gctgcggcgg atcgcccggg aggatggcgc cgcgcccgcc gtctcctccg
     2221 tgctcgtggt caatgaggag cgttttgtcg tgcactatca cggc
//
LOCUS       DATDAY010000371         4729 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_2621485, whole genome shotgun sequence.
ACCESSION   DATDAY010000371 DATDAY010000000
VERSION     DATDAY010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 4729)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4729)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4729
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2621485"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..984)
                     /gene="rfbB"
                     /locus_tag="VIS74_07335"
     CDS             complement(<1..984)
                     /gene="rfbB"
                     /locus_tag="VIS74_07335"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HEY5793094.1"
                     /translation="MNLLVTGGCGFIGSNFVRQRLTEASSPLTRLVNLDLLTYAGNPA
                     NLADLAADPRYIFVRGDIGDEPLAARLLIENGIDAVVNFAAESHVDRSIDSPEPFVQT
                     NALGTLRLLNAACRYWRGLPEPQKSIFRFLHISSDEVYGTLSPTDPAFTEETPFAPNS
                     PYAASKAAGDHLVRAFQHTYGFPTLTTHCSNNYGPYHFPEKLIPLVILNALEGNALPV
                     YGDGRQVRDWLFVEDHAAAIWRVVQRGVPGETYNIGGRNEKPNLEIVRTVCALLDRKS
                     PRPDRVSYAAQITHVADRPGHDRRYAINCDKIQRELGWEPRESFATGIEKTV"
     gene            complement(1013..1885)
                     /locus_tag="VIS74_07340"
     CDS             complement(1013..1885)
                     /locus_tag="VIS74_07340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar nucleotide-binding protein"
                     /protein_id="HEY5793095.1"
                     /translation="MIYLLGGSGYVGQAYQQLLARKGIPYRNLRRAELDYTDPATLIA
                     ALKADRPEFLINAAGYRGMPNVDACELHKADCLFGNAVLPGRIAEACLAAGVPWGHVS
                     SGCIYTGSRSDGGGFTEEDPPNFTFRQNNCSFYSGTKALGEEVLAGVPSVYLWRLRIP
                     FDHTDSPRNYLTKLMRYQRLLEATNSISQLHEFAAATLACWEKRAPFGTYNVTNPGQV
                     TTREVVDLIRASGVCTKEFAFFSDESEFMQKAAKAPRSNCTLSSAKLASAGIRMTDVR
                     EAVPKALANWKREI"
     gene            complement(2047..3069)
                     /locus_tag="VIS74_07345"
     CDS             complement(2047..3069)
                     /locus_tag="VIS74_07345"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY5793096.1"
                     /translation="MLHFQKSSASSLHRRLPALTSAYSLRQALGFPPVESCLSFGCAT
                     GEEVFSLCALFPQATVHGCDISETALSLAQSAGLNLRAEFFNSNWGEIEARGPYDIIF
                     ANAVFCYHHQSIFLDSLDADFPFPSFVDATARLVSLLRPGGLLLMQDTTYPLALTPAA
                     GIMIPIQLPGETWSGVVEKFGPDGLKFGKRHFAGFQMAFERIREFPAPFPESFVDTAW
                     IKCEKADPPERFRVEKTCRQALEQPTFLGSYFRDTADELSVANLINQRTLASFYRSAD
                     GVIIQEEHKCLVDSRENQTVFEEKMASVIDPANAAPVFLPEWIDTRFWEQRAGQTSWT
                     QLQAQE"
     gene            complement(3110..4321)
                     /locus_tag="VIS74_07350"
     CDS             complement(3110..4321)
                     /locus_tag="VIS74_07350"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY5793097.1"
                     /translation="MLFRSKHVELIYQEGESDCIIVTFNGIGSRADGKRFWGDTFLKK
                     NGLSAMGFVSTHRNWFPRESMDELIEIALPILKNYKQVCTYGASMGAYGAIKFSRRLG
                     AHTVVAFSPQYTIDPSQKHTAFTYFYNAELNQGVTIEAEDIGGNVFLFFDPTHLVDNY
                     AANYIAKLCNANLVPVPYIGHTTIVPFAKSGRGLEVLKLSMAGEPKPLRQLSQHTRRN
                     HGGDLRVFHLALRLIEKHISLALAVCEKYPQILDEQRLVAFSRELQRFHQIGPAIAWA
                     QASVDRDPARANAFRNLSGFLRASGNLAEAKQALHKALQIEPANHQFMMKLSIICCDL
                     QQIDEGIDWARKAIEAAPHDPQSFYNLVLIQKNNQPTDQAIETLQQALIRHPRNNSFK
                     VLLEELQNAQS"
     gene            complement(4385..>4729)
                     /locus_tag="VIS74_07355"
     CDS             complement(4385..>4729)
                     /locus_tag="VIS74_07355"
                     /inference="COORDINATES: protein motif:HMM:NF024579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5793098.1"
                     /translation="ISGNLPEAKRVIARALELEPNKYAYMTTFSQICLDAGDHEEAID
                     WARKATQICPVDPQTFRHLALTCQLSGSSERALAALKEAQTFHPNNSPLMELMEAIKL
                     NKDIHDELDAST"
BASE COUNT         1012 a         1229 c         1417 g         1071 t
ORIGIN      
        1 cacggttttt tcgatgcccg tggcgaacga ttcccggggc tcccagccga gctcgcgctg
       61 gattttatcg cagttgatcg cataccgccg gtcgtggccg ggccggtccg cgacatgagt
      121 gatttgcgcg gcataagata ccctatcggg ccggggagat ttccgatcca gcagggcgca
      181 gaccgtccgg acgatttcga ggttgggctt ttcattccgc ccgccgatat tgtaggtttc
      241 gccgggcact cccctttgaa cgacccgcca gatggcggcg gcgtggtcct ccacaaacag
      301 ccagtcccgg acttgccgcc catccccgta aaccggaagc gcgttgccct ccagggcatt
      361 gagaatgacc agcggaatca acttttccgg gaaatggtag gggccgtagt tgttggagca
      421 atgggtcgtc agagtcggga agccgtaggt atgttgaaag gcccggacga ggtgatcgcc
      481 ggccgccttg gaagcggcgt agggggaatt gggcgcgaag ggagtttcct ccgtgaaggc
      541 gggatccgtc ggcgagagcg tgccatagac ttcgtccgag gagatgtgca ggaaccggaa
      601 aatggatttt tggggttccg gcaagccgcg ccagtatcgg caggcggcgt tgagtaatcg
      661 cagggtgccc aacgcattgg tttgcacgaa cggctccggc gagtcgatgg accgatccac
      721 atgggattcc gccgcgaagt tgaccacggc atcgatccca ttttcgatca gcaatctggc
      781 agcaagcggc tcatcgccaa tgtctccccg cacaaaaatg tatcgcggat ccgccgccag
      841 atcggccagg ttggcgggat tgcccgcgta ggtcagcaga tcgaggttca ccaagcgggt
      901 caggggcgaa ctggcctcgg tgaggcgctg gcgaacaaaa ttgctgccga taaatccgca
      961 accgccggtg acaaggaggt tcatatgggt ggtttgggtg cttgccggtg tcttagattt
     1021 ctcgcttcca gttggccagc gctttgggga cggcctcgcg cacgtcggtc atccgaattc
     1081 cggcactggc cagctttgcc gaagacagcg tgcaatttga acgcggcgcc ttggcggcct
     1141 tttgcataaa ttcgctttcg tcggaaaaaa acgcaaactc cttggtgcag accccgcttg
     1201 cgcgaatcag gtccacgact tcacgcgtgg tcacctgccc ggggttggtg acattgtagg
     1261 tgccgaaagg cgcccgcttc tcccagcagg cgagggtggc cgccgcaaat tcatggagct
     1321 gggagatcga gttggtggct tcgaggaggc gctgataccg cattaacttg gtcaagtaat
     1381 tgcgcgggct gtcggtatgg tcaaacggaa tccgaagccg ccagaggtaa accgagggga
     1441 ctccggccag gacttcttcg cccagcgcct tggtcccgct gtaaaaggaa cagttattct
     1501 ggcggaaggt gaaattgggc ggatcctctt cggtaaatcc accgccatcg gatcgcgagc
     1561 cggtatagat gcatccggag gagacgtgcc cccacggcac tcccgcggcg aggcaggctt
     1621 cggcaatccg tcccggcagg acggcgttcc cgaagaggca atccgccttg tgcaactcgc
     1681 aggcatccac attcggcatc ccccggtaac cagctgcatt gatcaaaaat tccggacgat
     1741 ccgcctttaa tgccgcgatc aaagtggcgg gatccgtgta gtccaattcg gcccggcgca
     1801 aattgcggta ggggattcct tttcttgcaa ggagctgctg atacgcctgc cccacgtaac
     1861 cggagccgcc gaggagataa atcatcgtat tatccgtgca gtttagtgcg ggtgttttct
     1921 actggctaga acaacccggc gttatcccct ttaccattgg cgtcgctggc aatccggaat
     1981 tttctccggg tgctggcacg aaattcagca gttttggaat cgtggccgct tttgggtgac
     2041 gggaagttat tcctgtgcct ggagttgagt ccaggacgtt tgcccggcgc gttgctccca
     2101 gaaccgggtg tcaatccatt cgggcaaaaa gacgggagcc gcgtttgcgg ggtcaatgac
     2161 actggccatt ttttcctcaa acacagtctg gttctcccgg ctatctacca ggcatttgtg
     2221 ttcttcctga ataatgaccc cgtccgccga acgataaaag gaggcaaggg tccgctggtt
     2281 gatcaaattc gccacggaaa gctcatccgc ggtatcccgg aagtaggatc ccagaaaggt
     2341 cggttgttcc aaggcctgcc ggcaggtttt ttccacccta aaacgttcgg gcggatcagc
     2401 tttttcgcac ttgatccagg cggtatccac gaacgactcc gggaaagggg ccggaaattc
     2461 tctgattcgc tcgaatgcca tttgaaatcc ggcaaaatgt cgcttcccga acttgagccc
     2521 gtccgggcca aatttttcca ccaccccgga ccaggtttcg ccaggcagtt ggatcggtat
     2581 catgatgcct gccgccggag tgagggccag tggataggtg gtatcctgca tcagcagaag
     2641 tcccccgggg cgaagaagac tgaccagcct ggccgtggca tccacgaagc tgggaaatgg
     2701 aaaatctgcg tccaggctgt ccaggaagat ggattgatgg tggtagcaaa agacggcatt
     2761 ggcaaaaatg atgtcatagg gcccccgtgc ttcgatttcg ccccaattcg agttgaaaaa
     2821 ctcagcgcgg agattgagcc cggctgattg ggcgagcgat aaagcagttt cactgatgtc
     2881 gcatccatgg accgtggcct ggggaaaaag ggcgcacagg gaaaaaactt cctccccggt
     2941 ggcacagccg aagctcaggc agctttccac tggcgggaaa cccagagcct gccgcaggga
     3001 gtaagcgctg gtcaatgcag gcagtctgcg gtggagactg ctggcggagc tcttctgaaa
     3061 atggagcatg tttttctcgg attacgtcgt gaattctccg cctatccgat catgactgag
     3121 cgttttgcaa ttcttccagt aaaaccttga aagaattgtt tcgggggtgc cgaatgagag
     3181 cctgttggag agtctcgatt gcctggtcgg tcggctggtt atttttttgg atcaaaacca
     3241 agttgtagaa agactgggga tcgtggggag ctgcctcgat ggcctttctc gcccaatcaa
     3301 ttccttcgtc gatctgctgt agatcgcagc agatgatgct gagtttcatc atgaactgat
     3361 ggttggctgg ctcaatttgc agcgctttgt gcaaggcttg cttggcttca gccagatttc
     3421 cgctcgccct gaggaacccg gaaagatttc tgaatgcatt ggctcgggcg ggatcgcgat
     3481 ccaccgaagc ttgagcccag gcaatggccg ggccgatctg atgaaaacgt tgaagctccc
     3541 ggctgaatgc caccaaacgc tgctcatcga ggatttgcgg atatttttcg caaacggcca
     3601 aagccaatga gatatgcttc tcgatcaatc gcagagccag atgaaagacc cggagatctc
     3661 cgccatgatt gcgccgcgta tgctgggaga gctgtcgcag cggtttaggc tcgccggcca
     3721 tgcttagctt taatacctcc agtccccggc cggatttcgc gaaaggcacg attgtggtat
     3781 ggccgatgta aggaacggga accaaattcg cattgcaaag tttggcgata tagtttgcgg
     3841 catagttatc aaccaagtgg gtcggatcaa aaaagaggaa cacgttccct ccaatgtctt
     3901 ccgcttcgat ggtgacgcct tggttcaact ccgcgttgta aaaataagta aaagccgtat
     3961 gcttttggct cggatcaatt gtgtattggg ggctgaatgc gaccaccgta tgagcgccca
     4021 gccgccggct gaatttgatg gcgccatagg ctcccatgct ggcgccgtag gtgcatacct
     4081 gcttatagtt tttaagaatg ggaagggcga tttcgatcag ctcgtccatg ctttcgcgcg
     4141 gaaaccagtt gcggtgcgtg gacacgaatc ccatggccga caggccattt tttttcagaa
     4201 aagtatcccc ccaaaaacgt tttccgtccg cccgggaacc gattccgtta aaggtaacaa
     4261 taatgcagtc ggattcccct tcttgataga tgagctcaac atgtttggat cggaaaagca
     4321 tggctggaaa aaatgagtga tggataagac ggtgggagac gggatgaaaa atgcggcaaa
     4381 acttttaagt cgaagcgtcg agttcgtcgt gtatatcttt gtttaattta atggcttcca
     4441 tgagttccat gagagggctg ttattgggat gaaaggtctg cgcctctttg agggcggcga
     4501 gtgccctttc ggaggaaccg cttagctggc aggtaagagc caagtgtcgg aaggtttgcg
     4561 gatccacagg gcagatttgg gtggctttcc gtgcccagtc gatggcttct tcatgatcgc
     4621 cggcatccag gcagatttgg ctgaaggtag tcatgtaggc atatttgttc ggttcaagct
     4681 caagggcgcg ggcgattact ctttttgcct ccggaagatt tccgctaat
//
LOCUS       DATDAY010000372         2155 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_1062386, whole genome shotgun sequence.
ACCESSION   DATDAY010000372 DATDAY010000000
VERSION     DATDAY010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2155)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2155)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2155
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1062386"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(12..815)
                     /locus_tag="VIS74_07360"
     CDS             complement(12..815)
                     /locus_tag="VIS74_07360"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HEY5793099.1"
                     /translation="MESWPAIFALSVLCLAGLAVYSRVVRTVLSRPADFLVVSKYRIG
                     DVAFIGTILFVLGGLYGMAALAEHLGGKRAELNLATIIVNCFFSLGLMGGILLFLGLR
                     GINPVEVFGLNRFGFRAAATALGLLVSAYPMVILAQVVTYSFTSPEARPQEVVTFLLE
                     SRQWAERLAIGALAVGVAPLTEELIFRGYLYGVLRKYRNRFWAMLVSAVTFAAIHVHL
                     PAMPGLFVLALVLAVAYELTGSLWTPILMHACFNAVSVYVAIFHPNIME"
     gene            complement(822..1817)
                     /gene="sppA"
                     /locus_tag="VIS74_07365"
     CDS             complement(822..1817)
                     /gene="sppA"
                     /locus_tag="VIS74_07365"
                     /inference="COORDINATES: protein motif:HMM:TIGR00706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HEY5793100.1"
                     /translation="MKKSGCLPIALFLGLCLSLFLNVLLGAALSARVKGKVAPRITAE
                     QRFIEQIVEPGAGSGKIAVIPLVGIIGFEQQNALGANLVDDFKAALQQAKEDPDVTAV
                     VVSTDSPGGEITASDVLYKAIQDFAKEKPVVYYMNSIGASGAYYAACGSTWMMCNDTT
                     FTGSIGVIISALNYRELFGKVGLESVVFKSGKFKDMLNGARELTPEESAYIQGLVMQS
                     YDRFLGIVADKRKLSADSLRSGPADGRILSGTDALAQKLVDQLGYIEDAYAKARELGE
                     SPEAEIVRYRRSFSFSSFFDLLGESRATKVELNLPGREVKLEPGRIYLLPALFVP"
BASE COUNT          473 a          685 c          621 g          376 t
ORIGIN      
        1 ctcggagatt ttcattccat gatattcgga tgaaagatcg ccacgtaaac cgagaccgca
       61 ttaaagcacg catgcatgag gatcggcgtc cacagcgaac cggtcagctc atacgccacc
      121 gcgagcacga gcgcgagcac aaacagcccc ggcatggcgg gcaggtgcac gtggatcgcg
      181 gcaaaggtca ccgccgatac cagcatggcc cagaaacggt tgcgatattt tcgcagcacg
      241 ccgtaaaggt aaccgcgaaa aatcagctcc tcggtcaagg gcgcgacccc gaccgccagc
      301 gcgccaatgg ccagccgttc cgcccattgc cggctttcga gcagaaacgt caccacctcc
      361 tgcggccggg cttccgggct ggtgaaggaa taggtgacga cctgcgcgag gatgaccatc
      421 ggatacgcgc tgacgagcag gccaagcgcg gttgccgcgg cgcgaaatcc aaaccggttc
      481 aggccgaaaa cctccaccgg attgatgccg cgcagtccga ggaacaacag gatgccgccc
      541 atgaggccca ggctgaagaa gcaattgacg atgatcgtgg cgagattgag ctccgcccgc
      601 ttgccgccca ggtgctcggc cagcgccgcc atgccgtaaa gcccgcccag cacgaaaagg
      661 atggtgccga tgaacgccac gtcgccgatg cggtatttgg acaccaccaa aaaatccgcc
      721 gggcggctca gcaccgtccg gaccacgcgg gaataaaccg ccaggcctgc caggcacaag
      781 accgaaagcg cgaagatcgc tggccagctt tccatggccg gtcagggaac aaacaaagct
      841 ggcagcaggt aaatccggcc gggttccagt ttcacttccc gtccgggcag gttcaactcc
      901 accttcgtcg cgcggctttc cccgagcagg tcgaaaaagg aggaaaaact gaacgaacgc
      961 cggtagcgca cgatttccgc ctccgggctc tcgcccaact cgcgggcctt ggcgtaggcg
     1021 tcctcgatgt aaccgagctg gtccaccagc ttctgcgcca gggcgtccgt gccgctgagg
     1081 atgcggccat ccgccgggcc gctgcgcagg ctgtcggccg aaagtttgcg cttatcggcg
     1141 acgatgccca gaaagcggtc gtaactctgc atcacgaggc cctggatgta ggcggattcc
     1201 tcgggggtca gctcgcgggc gccattgagc atgtccttga acttgccgct cttgaagacc
     1261 accgactcca gccccacctt gccaaacaac tcgcggtagt tgagcgcgga aataataacg
     1321 ccgatgctgc cggtgaacgt cgtgtcattg cacatcatcc acgtcgatcc gcaggcggcg
     1381 taatacgctc ccgaggcgcc gatcgagttc atgtaataca ccaccggctt ttccttggcg
     1441 aaatcctgga tggccttgta gagcacgtcg gaggcggtga tctccccgcc cgggctgtcg
     1501 gtggagacca ccacggcggt gacatccgga tcttccttcg cctgctgcaa ggccgccttg
     1561 aaatcatcca ccagattcgc gcccagcgcg ttttgctgct caaacccgat gattcccacc
     1621 agcggaatga ccgcaatttt tccgctgccc gcgcccggtt ccacgatttg ctcgatgaat
     1681 cgctgctcgg cggtaatccg gggagccacc ttgcccttca cccgggcgct tagggccgcg
     1741 cccagcagga cattcaaaaa caaactcagg cacaggccca aaaacaaagc gatgggcaga
     1801 caaccggatt ttttcatgag cgaagcgcaa gataccgaat tcctcccccg tctcaagcag
     1861 cgattcgccg ccggtccgca attgtgcttt cccccctgtg aataagttga gaagaagagt
     1921 cggcggcttc tgaattttat tcacaacgcc cctgtgaaaa ttttgcgaac acgcgaatga
     1981 ttacccaatg aatttaaatg agatacggga tattctccgc cgcatgggcg aggatccctc
     2041 ccgcgcgctg ggccagaact ttttgattga tgaaaacttt gcggcctgga tggccgggca
     2101 actggaaatc cagcccggcg accgggtgat cgagatcggc cccggcctgg ggtcg
//
LOCUS       DATDAY010000373         2014 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_2530485, whole genome shotgun sequence.
ACCESSION   DATDAY010000373 DATDAY010000000
VERSION     DATDAY010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2014)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2014
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2530485"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..92)
                     /locus_tag="VIS74_07370"
     CDS             complement(<1..92)
                     /locus_tag="VIS74_07370"
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEY5793101.1"
                     /translation="MSQPKILAFAGSTRAESFNKKLVRLAAEAAR"
     gene            complement(308..1657)
                     /locus_tag="VIS74_07375"
     CDS             complement(308..1657)
                     /locus_tag="VIS74_07375"
                     /inference="COORDINATES: protein motif:HMM:NF012427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY5793102.1"
                     /translation="MNPSSFEPTERFQSKLHFLFATWEGGGNIPPAISVARKLARKGH
                     RVSFMSDSCSRAAAEAAGLEFVSWEKAPNRADKTPATCPVRDWEATEPMGDIALLRDR
                     IMFGPAGLYARDVLKFTACERPDLIVSSEMLPGVLLAAEAGGTPCVMLGGNVSFVPIP
                     GIPPLGPGLAPARNEEERAQHAAIEQHTAEFFNAGLPDLNAARASLGLAPLADVAGQF
                     RAASHYLLATSPSFDFPADFLPPHIDYVGPQLEDPDWARAWDSPWSADDPRPLVLVAF
                     STTFMDHGDTIRETLQALARLPVRGLATLGPGMAGESFPVPANVHLCASAPHSLLLRS
                     ASAVITHAGHGTTIRSLAAGVPLLCLPMGRDQNDNAIRVTARGAGISLSRHAPAEEIE
                     RAMRELLTEPSYRKAAQALGAAIVRDAERLDAASRIEDLAYETLTLRPNEPLAAGIA"
     gene            complement(1694..>2014)
                     /locus_tag="VIS74_07380"
     CDS             complement(1694..>2014)
                     /locus_tag="VIS74_07380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5793103.1"
                     /translation="LVGDYEITGDLVLRLLAQEERHPAFQPLLQRGRHAHEAWVNRIF
                     SEPLDALTGAARDARVAQLVAATDVFTWKLWRRDRGMPARKVADLMFDMTARLNAPAA
                     GRSR"
BASE COUNT          350 a          623 c          713 g          328 t
ORIGIN      
        1 cgggcggcct cggcggcgag gcggacgagt tttttgttga aggactcggc gcgggtgctg
       61 ccggcgaagg cgaggatctt tggctggctc atggttggaa aggcatgggc atcaggctag
      121 cagaatttcc ggaatgcgca gcaattcccc aacacaattc cccgagcggc caggcttgaa
      181 acggacatcc ccgcggggtg tggggcgctt cggcgccggt gatttccgaa acgtgccccc
      241 gaaccggctt cgttcaagtg gcggatgccg aatcgcgtgc cgcggctgta tccgccgcgg
      301 gggacggtta ggcaatcccc gcggcaagcg gctcgttcgg tctgagcgtg agcgtttcgt
      361 aagccaggtc ttcgattcgc gacgccgcgt cgagtcgttc cgcgtcccgc acgatcgccg
      421 cgcccagcgc ttgcgcggct tttcggtagg agggctcagt cagcagctcc cgcattgccc
      481 gctcgatttc ctccgccggc gcgtggcggg agagactgat tccggcgccg cgcgcagtca
      541 cccgtatggc gttgtcattt tgatcgcgtc ccatgggcag gcaaagcagc ggaactccgg
      601 cggcaagcga gcggatggtc gtgccgtgtc cggcatgggt gatcaccgcg gaggctgagc
      661 ggagcagaag ggaatgcggc gcgctggcgc aaaggtgcac gtttgccggg acggggaacg
      721 attccccggc catgcccggc cccagggtcg ccagtccgcg aaccggaagc cgcgcgagcg
      781 cttggagcgt ctcgcgaatc gtatccccat ggtccatgaa agtcgtgctg aacgccacga
      841 gcacgagcgg gcgcggatcg tcggcggacc agggggaatc ccacgcgcgg gcccagtccg
      901 gatcctcgag ctgcgggccg acatagtcga tgtgcggcgg gagaaaatcc gcggggaagt
      961 cgaagctcgg actcgtggcc agaagataat gcgacgcggc gcgaaattgg ccggccacgt
     1021 cggcgagcgg cgccaggccc agcgacgccc gcgcggcatt gagatccggc aggccggcat
     1081 tgaaaaactc cgccgtatgt tgctcgatgg ccgcatgttg ggcgcgttcc tcctcattcc
     1141 gggccggggc caagccgggg ccaagcggcg ggatgccggg aatggggaca aatgaaacat
     1201 tcccgcccag catcacgcaa ggcgtgccgc cggcttccgc ggcgagcagg acgccgggca
     1261 gcatttcgct cgaaacgatg aggtcgggcc gttcgcacgc cgtgaacttg agaacgtccc
     1321 gcgcgtaaag gccagccggg ccgaacatga tgcggtcgcg aagcagcgcg atatcgccca
     1381 tcggttcggt cgcctcccaa tcccggacgg gacacgtggc gggagttttg tcggcccggt
     1441 tcggggcttt ctcccaactg acaaactcca ggccggccgc ctcggccgcg gcgcgactgc
     1501 aggaatcgct catgaaggaa acgcgatgcc ccttccgggc aagcttgcgg gccaccgaga
     1561 tggcgggcgg gatgttgccg ccaccctccc aggtggcgaa caggaagtgc agtttcgatt
     1621 ggaatcgttc ggtaggttca aaggatgaag gattcatggt ttttttaatg gggtgttgga
     1681 gaaagcaaga aggtcatcgc gagcggccgg cggccggggc gttgaggcga gccgtcatgt
     1741 caaacatgag atccgcgacc tttcgcgccg gcattccgcg atcgcggcgc cacagcttcc
     1801 aggtgaatac gtcggtcgcc gcgaccagct gcgcgacccg cgcgtcgcgc gccgccccgg
     1861 tgagtgcgtc caacggctcg gaaaagatcc ggttcaccca ggcttcgtgg gcgtggcgtc
     1921 cgcgctgcaa gagcggctgg aacgccggat gccgctcttc ctgcgcaagc aggcgcagca
     1981 cgaggtcgcc ggtgatttca taatcgccca ccag
//
LOCUS       DATDAY010000374         2109 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_2084835, whole genome shotgun sequence.
ACCESSION   DATDAY010000374 DATDAY010000000
VERSION     DATDAY010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2109)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2109)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2109
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2084835"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            391..681
                     /locus_tag="VIS74_07385"
     CDS             391..681
                     /locus_tag="VIS74_07385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5793104.1"
                     /translation="MLPSQYASLAVRAGQPTFFYLPTTESSANQLPAAVEIYMIAASS
                     AETKRLGANLPAMPSIANAEALPAAVNAYLDALVNAGLKSAELYSTTVTLDH"
     gene            688..>2109
                     /locus_tag="VIS74_07390"
     CDS             688..>2109
                     /locus_tag="VIS74_07390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5793105.1"
                     /translation="MLTIPTPSRRNSAGFALLLALVMIVLATVLAVAFLTSSRINRSA
                     SNTTANSERIDLAAQSAVEHAVGILDSNIPQPRPPGAPLDNPVNWITSPGLLTLVDGS
                     GTRRIPLSSNPDSTYTPSRTDADLNSRLLRPDASGQRGYPIAAPKSGDSLPLRVAWVN
                     LLQNPALPASAANPIVARYGFWIDDESTRLNLNMAYGKPARALNYDNPHPLCPANYGN
                     WNGREKSGTPQYGPAYFMQPEFVDGTQSANALPTNRILWNTGEKKLEPTKYPLWHPAS
                     LELPVLNAAGYTVERDKLTDWIHNKFQGVSFQYQWRTLASPIDVLQYVKEEPPLATKT
                     AAAAAFWRDNQFNITTTGRSPEFNVWGKSRLFMEREMRDDVAAKFYQGSYDKDGPAYF
                     GAETSAGTYDSAPTATRGEWYPNLSGLYAAANSISNLLNRNDWPGLEGASFVDKWGGG
                     ELGKREADQVAWNIAIMGAYSPSR"
BASE COUNT          438 a          680 c          578 g          413 t
ORIGIN      
        1 gaaccgcgag caaagaaacg ccgccccaaa aactaccggc tcctcaccaa accccggcac
       61 caaatgaagc tctcccaaca ccgaaatcgc tggaaggcta agtctcccaa acccgcctta
      121 tcttaacgcc attcgggcca gccccgcatt accggcgcca gtccgacgaa tgatttcgcg
      181 cgcgccgacc attggattta ccgccagcct ttgttttgtg aagcgactcc cggagtcacg
      241 ccgcccaatg ggtttgcgac gttggtttcc gaaaaaatta ttggcttttg gattcggtgt
      301 cttgatggag cccgcaaacc gatcccgtgg ttgtcgcagc atgacaattc gtcggcgccg
      361 ctgaagttca attccgcggc ccgttttcaa atgctcccct cccagtatgc ctcgctggcc
      421 gtgcgcgcgg gccagccgac gttcttttac cttcccacca cggaaagttc cgccaatcag
      481 cttcccgcgg cggtggagat ctatatgatt gccgcgagca gcgccgaaac gaagcggctg
      541 ggcgccaatc ttccggccat gccgtccatc gcgaacgccg aggcattgcc cgcggcggtg
      601 aacgcctatc tcgatgcgct cgtcaacgcc ggtctaaaat cggcggagct ttattcgacg
      661 accgtcaccc ttgatcactg atttatcatg ctgacgattc cgactccctc ccgtcgcaac
      721 tccgccggtt tcgcgctcct gcttgccctc gtaatgatcg tgctggcgac tgtgcttgcc
      781 gtcgcattcc tcacgtcttc gcgcatcaac cggtccgctt caaacacaac ggcaaacagc
      841 gagcgaatcg acctcgccgc gcagtccgcc gtggagcacg cggtgggaat tctggacagc
      901 aacattccgc aaccccggcc gcccggcgcg ccgctcgaca atcccgtaaa ctggatcacg
      961 agtcccggtc tgctcacgct ggttgacggc agcgggacgc ggcgcatccc cctcagttcg
     1021 aatcccgatt ccacttacac gccatcccgc acggatgcgg accttaattc ccggctgctg
     1081 cggccggatg cgagcggaca acgcggttat cccatcgccg cgccgaagag cggcgactcg
     1141 ctccccttgc gcgtggcctg ggtgaatctt ctccagaacc ccgccttgcc cgcttcggcg
     1201 gccaatccga ttgtggcccg ttacggtttt tggatcgacg acgaatcgac ccgcctgaat
     1261 ctgaacatgg cgtatggaaa gccggcaaga gccctcaatt acgacaaccc tcatccgctc
     1321 tgccccgcga attacggaaa ctggaacgga agggagaaat cgggcacgcc tcagtatggt
     1381 cccgcctatt tcatgcagcc ggaattcgtg gatggcaccc agtccgccaa cgcgctgcca
     1441 accaatcgca tcctttggaa tactggagag aagaagcttg aacccaccaa atatccgctc
     1501 tggcatcccg cctctttgga attgcccgtg ctcaatgcgg ccggatatac cgtagagcgg
     1561 gacaagctca cggactggat tcataacaaa ttccagggtg tgagctttca ataccagtgg
     1621 cggactctgg ccagtccgat tgacgttctt caatacgtga aggaggagcc gccgctggcg
     1681 acgaagacgg cggcggcagc cgcgttctgg cgggacaatc aatttaacat cacgactacc
     1741 ggccgatctc ccgaatttaa tgtctgggga aaaagccggc ttttcatgga gcgcgagatg
     1801 cgggatgacg tcgccgcaaa gttttaccag ggctcctacg acaaggatgg gccggcctac
     1861 tttggagcgg agaccagtgc gggaacgtat gacagcgctc caactgccac tcgcggcgaa
     1921 tggtatccaa atctttcggg cctttacgcg gcggccaaca gcatctcgaa cttgctgaat
     1981 cgcaacgact ggcccggact ggagggcgcg agttttgtgg acaaatgggg cggtggcgag
     2041 ctcggcaagc gagaggctga ccaagtggct tggaacattg ccatcatggg cgcatactcc
     2101 ccaagccgg
//
LOCUS       DATDAY010000375         5095 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_2076592, whole genome shotgun sequence.
ACCESSION   DATDAY010000375 DATDAY010000000
VERSION     DATDAY010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 5095)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5095)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5095
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2076592"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3231
                     /locus_tag="VIS74_07395"
     CDS             <1..3231
                     /locus_tag="VIS74_07395"
                     /inference="COORDINATES: protein motif:HMM:NF012907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 18 protein"
                     /protein_id="HEY5793106.1"
                     /translation="ENGEMVHSAAAPAADGQAQTASKKFTDRKYSANRYRVLLTNGSG
                     QTWSAEAGFTTDGASEILLPESDTGSQVRQVTLDLGKTQSFTLEMLDRKTANFKVATN
                     NATVVAASIANGKLQLRARAAGRAGVRLTDTLSGKVRYLGVRVKDAAGKLPGLPKYVS
                     MGSVSEDSVEDLTFWRSFEGSGTKNKRMDVRYIYLNGGPKAAGGGWRTWSQEDGFRAT
                     SFIRESLKLGMIPFFVWYNIPDGGESYWTDKQHMQDAAYLKGYFGDLKFALELCKAEA
                     GDELVGFVLEPDFIGYMAQNHENPQTLMAATAAAYETGFLERGKDPAFPDTMRGLVEA
                     INYGIKKHLPRAFMGWQVNLWASPAGGWTTPIGAKGVIRLTDERGVTQGRKDIYKEAS
                     AIADYYIQSGVLTHNADFLSIDKYGLDAGYEGKNDDPAKSTWFWNALHWTNYLSFCQA
                     LHDKSQKPVLLWQIPVGHINVSQEPGFPELNNTTRHYEDSAPTYFLGDKFLAGGQRLD
                     HFHRDDSVSKVTLEEGAVVWASHLDLTQKAGVISVLFGAGVGDSTDGVGTPPSDSGWW
                     IAKVQKYYAAPVKLDQSYGTGVPRPSPTPSATPAPSATPTPSATPAPGASPSPSATPK
                     PTPSATPTPSATPKPTPSATPKPTATPVATPTPTPTPTPTPTPTPTPTPTPAPSPSPT
                     PDPVGGPVPGNLIVGYFVEWGIYQRSYRVTDSPAKKLNVINYAFANVSDSGEVVLYDS
                     YAATEYSYPGDTWNQPLRGNFHQLKKLKAANPHLRTLISVGGWTLSGKFSDVALTGAS
                     RQKFAKSAVKFIRDYGFDGVDIDWEYPAGGGLSSNKSRPADKQNYTLLLQELRRQLNL
                     AGKSDGKKYYLTLAAGAGADKIANLEVAKIAAIVDWINIMTYDFHGSTWETTTGHHAG
                     LYSDRDSLSGDAAVRGFLAAGAPARKLTLGLGFYGRGWRGIPATRNGLYQTSTNPSWD
                     GTATSQGTFEAGVLDYWDIAGRLRREPSVYKRHWDDTAKVPWVYAPALEGGTFYTYDD
                     PESLRLKVQYLKQKGLGGAMFWELSGDEKAGSHPGLLDAVNEEMAK"
     gene            complement(3259..3450)
                     /locus_tag="VIS74_07400"
     CDS             complement(3259..3450)
                     /locus_tag="VIS74_07400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5793107.1"
                     /translation="METKTKAFDAVAESRKWREAASRKLNAMSLEERLEYLRQVRERY
                     AAEYAARHAESGHATASSA"
     gene            complement(3425..3898)
                     /locus_tag="VIS74_07405"
     CDS             complement(3425..3898)
                     /locus_tag="VIS74_07405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5793108.1"
                     /translation="MRIPSLYLDTSVLGGYFDDEWQEPTQELWRQMEEGQWRFVCSDV
                     TTEEIEGAPERVRLLFFNTFPPETLLATTDEMEVLAAACVVQGVVTGKYQDDARHVAA
                     CTVSGLNLLVSWNFRHLVSLQRETGFNAVNLLQGYQIIRIVSPLELIYGNQDKSL"
     gene            4036..4284
                     /locus_tag="VIS74_07410"
     CDS             4036..4284
                     /locus_tag="VIS74_07410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5793109.1"
                     /translation="MAEMTEQERMRRWVETWKQAGPELQRIKEEELRARSDEQLARDS
                     EAALLLADHWLALHPGYERESGMIEQQRLFRKWTPPRI"
     gene            4263..4850
                     /locus_tag="VIS74_07415"
     CDS             4263..4850
                     /locus_tag="VIS74_07415"
                     /inference="COORDINATES: protein motif:HMM:NF020419.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HEY5793110.1"
                     /translation="MDATPDLSLVLKAALAVQDFCETNSWPYCFIGGLAYQIWGYPRS
                     TMDADLTLLTEFVRDEDYVDKLLARFESRRPDGREFALKYRVVLLWSDERVAIDIGLG
                     ALPFEANSIRRSVKRAIAPNVLLRVCSPEDLIVHKAFATREQDWADVDNVLLRQGRGL
                     DCAQIFEELRPLVALKEDDSILPRLEGMMRKRGIL"
     gene            4847..5080
                     /locus_tag="VIS74_07420"
     CDS             4847..5080
                     /locus_tag="VIS74_07420"
                     /inference="COORDINATES: protein motif:HMM:NF020967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HEY5793111.1"
                     /translation="MITLARFEKPEEAHLVRMRLEAGGVPAFIQDENSIQIQWMYSNA
                     LGGVRLQIAEEDFERAKEILNEPPLENVPDFPG"
BASE COUNT         1020 a         1754 c         1457 g          864 t
ORIGIN      
        1 gaaaatggcg agatggttca ttccgccgcc gctcccgccg cggacggcca ggcccagacg
       61 gcctcgaaga aattcaccga ccgcaaatac agtgcgaacc gttaccgcgt gctgctcacc
      121 aatggctccg gccagacctg gagcgccgag gccggtttca ccacggacgg cgcgagcgaa
      181 atcctcctgc ccgaatccga caccggctcg caagtccgcc aggtgaccct ggatctgggc
      241 aaaacccaat cgttcaccct cgaaatgctc gaccgcaaga cggcgaactt caaggtggcc
      301 acgaacaacg ccaccgtcgt cgccgcgtcc atcgccaatg gcaagctgca gcttcgcgcg
      361 cgggccgcgg gccgggccgg cgttcgcctc accgacaccc tttccggaaa agtccgctac
      421 ctcggcgtcc gggtcaagga cgccgccggc aagctgcccg gcctgccgaa gtatgtctcc
      481 atgggctccg tcagcgagga ctccgtggag gacctgacct tctggcgcag cttcgaaggc
      541 tccggcacga aaaacaaacg catggacgtg cgttacattt atctcaacgg cggcccgaag
      601 gcggccggcg gcggctggcg cacctggtcc caggaagacg gcttccgcgc gacctcgttc
      661 attcgcgaaa gcctcaagct cggcatgatc cccttcttcg tctggtataa catccccgac
      721 ggcggcgaga gctattggac cgacaagcaa cacatgcagg acgcggccta cctgaagggc
      781 tacttcgggg atctcaagtt cgcgctcgag ctctgcaagg cggaagccgg tgatgaactc
      841 gtcggtttcg tgctcgaacc cgacttcatc ggctacatgg cgcagaacca cgagaacccg
      901 caaacgctca tggccgccac cgccgccgcc tacgaaactg gattcctcga gcgcggcaag
      961 gatcccgcct tccccgacac gatgcgcggc ctcgtcgaag ccatcaacta cggcatcaag
     1021 aaacacctgc cgcgcgcctt catgggctgg caggtcaatc tctgggcctc ccccgccggc
     1081 ggctggacca cgcccatcgg cgcgaagggc gtcattcgcc tcaccgacga acgcggcgtc
     1141 acccagggcc gcaaggacat ctacaaggaa gcgtccgcca ttgccgatta ttacatccaa
     1201 tccggcgtgc tcacgcacaa cgccgacttc ctctccatcg acaaatatgg cctcgacgcc
     1261 ggctacgagg gcaagaatga tgacccggcc aaatccacct ggttctggaa cgccttgcat
     1321 tggaccaatt acctgagctt ctgccaggcg ctgcacgaca agtcccagaa gccggttctc
     1381 ctctggcaga ttcccgtcgg ccacattaat gtctcccagg agcccggttt cccggagctt
     1441 aataatacca cgcggcacta cgaggactcc gcgcccacct atttcctcgg cgacaaattc
     1501 ctcgccggcg gccagcggct ggatcacttc caccgcgacg actccgtgag caaggtcacg
     1561 ctcgaggagg gcgccgtggt ctgggcctcg cacctcgatc tcacccagaa agccggggtc
     1621 atctccgtgc tcttcggcgc cggcgtcggc gacagcaccg acggcgtggg gactccgccc
     1681 agcgacagcg gctggtggat cgccaaggtg caaaaatatt acgccgcgcc cgtgaagctc
     1741 gaccaatcct acggcaccgg cgtgccgcgg ccgagcccca cgccctcggc cacgcccgcg
     1801 cccagcgcaa cgcccacgcc gagtgcgacc cccgcgcccg gcgcgagccc ttcacccagc
     1861 gcgacgccca aacccacccc gtccgccaca ccgactcctt cggccacgcc caagccgacc
     1921 ccatcggcca cgcccaaacc cacagcgaca ccggtcgcga cacccacgcc tacgccgaca
     1981 cccacgccta cgccgacacc cacgcctacg ccgacaccca ccccggcgcc cagcccgagc
     2041 cccacgccgg atccggtcgg cggccccgtg cccggcaatc tcatcgtcgg ctacttcgtg
     2101 gaatggggca tctaccagcg gagctatcgc gtcaccgaca gtcccgccaa aaaactgaac
     2161 gtcatcaact acgccttcgc gaatgtctcg gactccggcg aagtcgtgct ctacgactcc
     2221 tatgccgcga cggagtattc ctaccccggc gatacctgga accaacccct gcgcggcaac
     2281 ttccatcaat tgaaaaaatt gaaggcggcc aatccccacc tgcgcaccct catctcggtc
     2341 ggcggctgga cgctctccgg caaattctcc gacgtggcgc tcaccggcgc ctcgcggcaa
     2401 aaattcgcca agtccgccgt gaaattcatc cgcgactacg gattcgacgg cgtggacatc
     2461 gattgggaat accccgccgg cggcggcctg agctcaaata aatcccgtcc cgccgacaag
     2521 cagaactaca ccctgctgct ccaggagctc cgccgccagc tgaacctcgc cggcaagagc
     2581 gatggcaaaa aatactacct caccctcgcc gccggcgccg gcgccgacaa gatcgccaac
     2641 ctcgaagtcg ccaagatcgc ggccatcgtg gattggatca atatcatgac ctatgacttc
     2701 cacggcagca cgtgggagac cacgaccggg catcacgccg ggctctacag cgaccgggat
     2761 tcactcagcg gcgacgcggc cgtccgcggt ttcctcgccg ccggagcccc cgcccgcaag
     2821 ctcacgctcg gcctcggctt ctacggccgc ggctggcgcg gcattcccgc cacccgcaac
     2881 ggcctctacc aaaccagcac caatccctcg tgggatggca ccgccacctc ccagggcacc
     2941 ttcgaggccg gcgtgctgga ttattgggac atcgccggcc gcctgcgccg cgaacccagc
     3001 gtgtataaac gccattggga cgacaccgcc aaggtgccct gggtctatgc cccggcgctg
     3061 gaaggcggca cgttctacac ctacgacgac ccggaatccc tccgcctgaa ggttcaatac
     3121 ctgaaacaaa aaggtctggg cggcgcgatg ttctgggaac tcagcggcga tgaaaaagcc
     3181 ggcagccacc ccggcctgct ggacgccgtg aacgaggaga tggcgaaata atcgatcatt
     3241 gaccgatccc cacttttgct aggccgagga agccgttgcg tggccgctct cggcgtggcg
     3301 ggccgcgtat tccgccgcgt agcgctcgcg gacttggcgc aggtattcca gacgctcctc
     3361 aaggctcatg gcgttgagtt tgcggctggc cgcctcccgc cacttgcggg attcggctac
     3421 ggcgtcaaag gcttttgtct tggtttccat agatcaactc caacggactt acgatacgga
     3481 tgatttgata tccttgcaaa aggttcacgg cgttgaaccc ggtttcgcgt tgcaggctga
     3541 ccagatgccg gaagttccaa ctcaccagca aattgagacc cgaaacggtg caggccgcaa
     3601 cgtggcgggc gtcgtcctgg tatttgcccg taacgacgcc ttgaacgacg caggcggctg
     3661 ccaagacttc catttcatcg gtggtcgcca gcagcgtttc cggagggaac gtgttgaaaa
     3721 agagcagtcg gacacgctcg ggagcgccct cgatctcctc ggtggtcacg tcggaacata
     3781 cgaaccgcca ctggccctcc tccatctgcc gccaaagctc ttgggtgggc tcctgccact
     3841 cgtcgtcgaa gtatccgccc aaaacggagg tatcgagata gagggacggt attcgcacgt
     3901 tcgggatcct accccttcgc ccggtctttg gcgatctgac aattgacctg tcttcctgtg
     3961 gcgctccgcc aaataaatcc ccgccgcgcc ccgcttttgc tcgtcgaaag cggggcgctc
     4021 ttttaggatg atgccatggc cgaaatgacc gaacaggagc gcatgcgccg ctgggtggaa
     4081 acctggaaac aagccgggcc cgagctgcag cggatcaagg aggaggagtt gcgtgcccga
     4141 agcgacgagc aactcgcccg ggattccgag gcggccctcc tgctggcgga ccactggctg
     4201 gccctccatc ccggctacga aagggaatcg ggaatgatcg agcagcagcg cctctttcga
     4261 aaatggacgc caccccggat ttgagcctcg tcctgaaggc cgccttggca gtgcaggatt
     4321 tctgcgaaac gaattcctgg ccttattgtt ttatcggcgg attggcctat cagatttggg
     4381 gctacccgcg atccacaatg gatgccgatc tcacccttct cactgaattt gttcgcgacg
     4441 aagactacgt ggacaagctg ctagcgagat ttgaatcccg acggccggat ggccgggagt
     4501 ttgccctgaa gtatcgcgtc gtcctgctct ggagcgacga acgggtggcc attgacatcg
     4561 gattgggcgc cctgcccttt gaagcaaaca gcatccggcg ttcggtcaag cgcgccatcg
     4621 cgcccaatgt gcttctgcgc gtttgcagcc cggaggatct gattgttcac aaagcgttcg
     4681 ccacccggga acaagattgg gcggatgtgg acaatgtgct cctccggcaa ggccgcgggc
     4741 tggattgcgc gcagattttc gaagaactcc gccccctggt cgcactcaag gaagatgact
     4801 cgattcttcc gcggcttgag ggcatgatgc gcaaacgagg aatcttatga tcacgcttgc
     4861 ccgattcgag aaacccgagg aggcgcatct ggtccgcatg cggctggaag cgggcggcgt
     4921 gccggccttc attcaggacg agaacagcat ccagatccaa tggatgtatt ccaatgcgct
     4981 cggcggcgtg cgcctgcaga tcgccgagga ggatttcgag cgggcgaagg aaatattaaa
     5041 cgaaccgccg ctggagaatg ttcccgattt tcccgggtag gtcaggcgcc cattg
//
LOCUS       DATDAY010000376         2195 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_672142, whole genome shotgun sequence.
ACCESSION   DATDAY010000376 DATDAY010000000
VERSION     DATDAY010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2195)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2195)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2195
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_672142"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..415
                     /locus_tag="VIS74_07425"
     CDS             <1..415
                     /locus_tag="VIS74_07425"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HEY5793112.1"
                     /translation="ARPEALAAALEALTLVRVKDLPAAVRLANQFAAEHVSIATREPG
                     NVAALLNSAGAIFLGGLSPVVAGDFLAGPSHTLPTGGAGKSFAGLTADQFQRRTSVIR
                     YDEAALRKSLPVLATFSEIEGLDAHGRSAAIRLPE"
     gene            412..1263
                     /gene="folP"
                     /locus_tag="VIS74_07430"
     CDS             412..1263
                     /gene="folP"
                     /locus_tag="VIS74_07430"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEY5793113.1"
                     /translation="MSGPYDWQLPGGRLDLTQRGLIVGILNVTPDSFSDGGQFLDAEK
                     AAARALEMERAGAGMIDVGGESTRPGAEPVPLAEEIARVIPVIRKIRETSAVRVSIDT
                     TKAAVAAAALEAGADVVNDITALRGDPDMPRVVAGSDCGIVLMHMRGTPRTMQQAPHY
                     ANVVEEVRDFLRQRITAAVEMGIEPMRLVVDPGIGFGKTPEHNRQLLARLDAFGELRR
                     PLYVGFSRKSFLKSLAGAESLEDRHWPGVALTSYCRERGARIFRVHDPKPHAQALRMT
                     EAILAHG"
     gene            1256..2041
                     /gene="cdaA"
                     /locus_tag="VIS74_07435"
     CDS             1256..2041
                     /gene="cdaA"
                     /locus_tag="VIS74_07435"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR00159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HEY5793114.1"
                     /translation="MAENLWNFIRENWSSPVEILVLAFVLYHTYLYLRGTQGARVLIG
                     LGLLVLGLTLLSQLLQLQVIGWILERLSVFIAIALVVIFQPELRRVLTDLGSHRFFRS
                     SYQEREMIELIVDAVFELASKGFGALIAIEREINLRTLADTGVDIDSSFTKELVLTIF
                     QPKTVLHDGGVIIRNDRVKAAACIFPLTQREDLDRNLGLRHRAGLGLAEQSDAIVIIV
                     SEETGQVSICQNGVIERNLNVEKFRRRLNELLVLEANEKPDSP"
     gene            2019..2165
                     /locus_tag="VIS74_07440"
     CDS             2019..2165
                     /locus_tag="VIS74_07440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5793115.1"
                     /translation="MKNLILHNWQAKLICLLLAIALWIAIKQNIPRPAIERRPPASLT
                     PKHP"
BASE COUNT          370 a          746 c          695 g          384 t
ORIGIN      
        1 cgcgcggccc gaggcgctgg cggcggcgtt ggaagccctg acgctcgtgc gggtcaagga
       61 tctgcccgcg gcggtgcggc tggccaacca atttgccgcg gagcatgtct cgatcgccac
      121 ccgcgagccg ggcaacgtgg ccgcgctgct gaactcggcg ggcgccattt tcctcggcgg
      181 gctgtcgccg gtggtcgcgg gcgatttcct cgccggtccc agccacacgc tgcccaccgg
      241 cggcgcgggc aagtcgttcg ccgggctgac cgcggaccag ttccagcggc ggaccagcgt
      301 gatccgttac gacgaggcgg cgttgcggaa gtcgctgccg gtgctggcaa ccttcagcga
      361 aatcgagggg ctcgacgccc acgggcgctc ggcggcgatc cgattgccgg aatgagcggc
      421 ccgtatgact ggcaactgcc cggcggccgg ctcgacctca cccagcgcgg gctcatcgtc
      481 ggcatcctga acgtcacgcc ggactcgttc tcggacggcg ggcaattttt ggacgcggaa
      541 aaggcggccg cgcgcgccct ggagatggag cgcgcgggcg cgggcatgat tgatgtcggc
      601 ggggaatcca cccggcccgg cgccgaaccg gtgccgctgg cggaggaaat cgcccgggtg
      661 attccggtca tccggaaaat ccgcgaaact tcggcggtcc gcgtttccat tgataccacc
      721 aaggccgcgg tggccgccgc cgcgctggag gcgggcgccg acgtcgtcaa tgacatcacc
      781 gccctgcggg gcgatccgga catgccgcgg gtcgtcgccg ggtcggactg cggcatcgtg
      841 ctcatgcaca tgcggggcac tccgcgcacc atgcagcagg cgccgcacta tgcgaatgtg
      901 gtcgaggaag tccgggattt ccttcgacag cggataaccg cggcggtaga aatgggcatt
      961 gagcccatgc gactcgtggt ggaccctgga atcggatttg gaaaaacgcc tgaacacaac
     1021 cggcagctgc ttgcccgcct ggatgccttc ggcgaactgc ggcgtcccct ttacgtgggt
     1081 ttctcccgca agtcgttcct caaatcgctg gccggcgcgg agtccctcga ggaccgccat
     1141 tggccggggg tcgccctgac cagctactgc cgggaacggg gcgcgcgcat tttccgcgtg
     1201 cacgatccca aaccccacgc ccaggcgctt cgcatgaccg aggcgatcct tgcccatggc
     1261 tgagaatctt tggaatttca ttcgcgaaaa ctggagctcg ccggtcgaga tcctggtgct
     1321 cgcctttgtc ctgtatcaca cctatctcta cctgcgcggc acgcagggcg cgcgcgtgct
     1381 catcgggctc ggcctgctgg tcctggggct cacgctgctt tcccagctgc tccagctcca
     1441 ggtcatcggc tggatcctcg aacgcctgtc ggtcttcatc gccatcgcgc tggtggtcat
     1501 ctttcaaccc gaactccgcc gcgtgctgac cgatctcggc agccaccggt ttttccgctc
     1561 gtcctaccag gaacgcgaaa tgatcgagct catcgtggac gcggtcttcg agctggccag
     1621 caagggcttc ggcgcgctga tcgccatcga gcgcgagatc aacctccgca cgctcgccga
     1681 cacgggcgtg gacatcgaca gctccttcac caaggaattg gtcctcacca tttttcaacc
     1741 caagaccgtg ctgcacgacg gcggagtcat catccgcaac gaccgggtca aggccgcggc
     1801 gtgcattttc ccgctcacgc agcgcgagga cctggaccgc aatctcggcc tgcgccaccg
     1861 cgcgggcctg ggactcgccg agcaatcgga cgcgatcgtc atcatcgtct ccgaggaaac
     1921 cgggcaggtg tccatctgcc agaacggcgt gatcgaacgc aatctcaacg tcgaaaaatt
     1981 ccgccggcgg ctcaatgagc tcctggtgct ggaagccaat gaaaaacctg attctccata
     2041 actggcaggc gaaactcatc tgcctgctgc tggcgatcgc gttgtggatt gccatcaagc
     2101 aaaatattcc ccgccccgcc atcgagcgcc gcccgcccgc ctcgttgact cccaaacacc
     2161 catgagcgaa tcccgaaaac tttttggaac cgacg
//
LOCUS       DATDAY010000377         2869 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_2269803, whole genome shotgun sequence.
ACCESSION   DATDAY010000377 DATDAY010000000
VERSION     DATDAY010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2869)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2869)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2869
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2269803"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(36..866)
                     /locus_tag="VIS74_07445"
     CDS             complement(36..866)
                     /locus_tag="VIS74_07445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5793116.1"
                     /translation="MRQSFLTKRSFSFSRNLVRISPFVCLFAPTGWAASEFGSTDSSR
                     AALIGTFYDLKQTQKLQPTGVTDAQYTEVLDEFLRKNWDEGVLNRYFRATQPLYTTQL
                     FIPSIDAAAAPKAFGVEKIVKPTMWVAHYKGQVIPPTDGAWRFWGQGDDILCVAVNGR
                     IVLINNWAGLRFPSIRWRSPEPTQVPCGKLKLAAGHWMKLKGGEPVDLDVLVGELPGG
                     LFAAYLMIEKRGDSDASAGGQRLFPIFQLAPYDPPLPTDPSGQAPRFLPNGPIWKGVQ
                     "
     gene            complement(947..2509)
                     /locus_tag="VIS74_07450"
     CDS             complement(947..2509)
                     /locus_tag="VIS74_07450"
                     /inference="COORDINATES: protein motif:HMM:TIGR02601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter-associated beta strand
                     repeat-containing protein"
                     /protein_id="HEY5793117.1"
                     /translation="MNKSSRKSSSTARDLLSRLASAALFAGPSLAQAADTYFDLNGAT
                     PGFGDYSVGGSNNWASGATNWTTSSAGDIATSAWVSGNAAIFDYANSSNANGSVRVSA
                     LEATPGNVAGISLINNTNNRTFTVGNGFVSALNLTIVGSSAVNITSGAFRLGNLINLQ
                     GSYTKTGAGDLQFTGTGGNAYSGTATLSAGRIVLENSVTGGLVGGIGANSNFVVNAGT
                     LGLMSNTGTYVIGSLSGSGGTISTGQDAVANNGSTHTLQVNQATSGTYAGTITQESTS
                     RVLALTKQGASTLTLSGSHTYTGATTINAGTLLVSSTGSIGAGSAVAVNSGGTLAGTG
                     NVNGTVTVNNGGTLSPGNSPGLQNLGSLALNNGGNYNWQILNAAGVAGTGYDSHNLSG
                     TLDLSGLTGAADFNLNLWSLASIGPDVNGDALNFINTQDYTWTLIATGFAITGFDATE
                     FTVNLGAINGTGGFSNALDGSFAVNLGDGGTDLVLTYTAIPEPATYLLLAGGLMTLLF
                     RRRRIRSFEPSR"
     gene            2612..>2869
                     /locus_tag="VIS74_07455"
     CDS             2612..>2869
                     /locus_tag="VIS74_07455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5793118.1"
                     /translation="MEISLVKLFTFYDYLPVGIVPGERTVASRFASLGAISDRLGGRR
                     WIFVRRHDAMIVEAFGAKPFFFSSLKKRKRRSHDLSSASTGG"
BASE COUNT          542 a          791 c          920 g          616 t
ORIGIN      
        1 tttcgcggaa gggaacggcg gtttcgcggg cggggttatt gcacgccttt ccagatggga
       61 ccgttgggca ggaagcgggg ggcttgcccg ctggggtcgg tggggagggg aggatcgtag
      121 ggcgccagtt gaaaaatggg aaacaggcgc tgcccgccgg cgctcgcgtc agaatccccc
      181 cgcttttcga tcatcaaata ggcggcaaag agtccgccgg gcagctctcc gaccaggacg
      241 tccagatcca ccggttcgcc gccctttaac ttcatccagt gccctgcggc cagcttgagc
      301 ttgccgcagg gcacttgcgt gggctccggc gacctccagc gaatgctggg aaaacgcagg
      361 ccggcccaat tgttgatcaa cacgatccgg ccgttgaccg ccacgcagag gatgtcatct
      421 ccctggcccc aaaaacgcca ggcgccgtcc gtgggtggaa tgacctgccc tttgtaatgg
      481 gccacccaca tcgtcggctt gacgattttc tcgacgccaa aggccttggg cgcggcggcc
      541 gcgtcgatgg acggaatgaa caactgggtc gtgtagagtg gctgggttgc gcggaaataa
      601 cggttcagga cgccttcgtc ccagtttttt cggagaaatt catccagcac ctccgtgtat
      661 tgcgcatcgg tgacgccggt gggttgaagc ttttgcgttt gcttgaggtc gtagaaagtg
      721 ccgatcaagg ccgcgcggct gctgtcggtc gagccgaatt cgctggcggc ccagcccgtc
      781 ggggcgaaga gacagacgaa cgggctgatc cggacaaggt ttcgggaaaa ggaaaatgat
      841 cgttttgtta ggaaggattg gcgcacaaaa aggtttttct ccgggtcctg acaacgtgaa
      901 cgcgggccgc cccccggcag cccgcgttcg ttagtctccc gcttttttaa cgagaaggtt
      961 caaatgagcg aatccggcgg cgccggaaaa gcagggtcat caatcccccg gcgagcagga
     1021 gataagtcgc gggctcggga atggccgtgt aagtgagcac gaggtcggtg ccgccgtcgc
     1081 ccaagttgac ggcaaagctt ccgtccaggg cgttgctgaa tccaccggtt ccgttgattg
     1141 ctcccaggtt gaccgtaaac tccgtggcat cgaaaccggt gatcgcaaat cccgtcgcaa
     1201 tcagcgtcca ggtataatcc tgcgtgttga tgaaattcaa cgcatcgccg ttcacatcgg
     1261 gtccgatgct cgcaagcgac cagagattga ggttgaaatc ggcggcgccg gtcagcccgc
     1321 tcaggtcgag ggttccgctc aggttgtggg agtcgtatcc cgttccggcc acgcccgccg
     1381 cattcagaat ctgccagttg tagttgcctc cattgttcaa tgccaggctt cccaggttct
     1441 gcaggccggg gctgttgcct ggcgacagcg tgccgccatt gttgaccgtc accgtgccgt
     1501 ttacgttgcc ggtgcccgcc agcgtgccgc cgctgttgac cgccaccgcg ctgcccgcgc
     1561 cgatgctgcc cgtgctgctt accaagagcg tgcccgcgtt gatcgtcgtc gcgccggtgt
     1621 aggtgtgtga gccggaaagg gtgagggttg atgcgccctg cttggtgagc gccagaacgc
     1681 gcgaggtgct ctcctgcgtg atggttcccg cataggtgcc ggaggtggct tgattgacct
     1741 gaagggtgtg ggtcgaaccg ttgttggcga cagcgtcctg ccccgtggag atcgtgccgc
     1801 cgctgccgct cagcgagccg atcacatacg tgccggtatt ggacatcagc ccgagcgttc
     1861 ccgcgttgac gacaaagttg gagttggcgc cgatgcctcc gaccagcccg ccggtgacgg
     1921 agttttccag gacaatccgg ccggccgaga gggtggccgt gccggagtag gcattgcctc
     1981 ccgtgccggt gaattgcaga tcccccgcgc cggttttggt gtagctgccc tgcaggttga
     2041 tcagatttcc cagccgaaac gctccggagg tgatattcac cgcgctggaa ccgacaatcg
     2101 tcagattcag cgcgctcaca aagccattgc caaccgtgaa cgttcgattg ttcgtattgt
     2161 tgatcagact gatcccggcc acgttgcccg gggtcgcctc aagggccgat acccggaccg
     2221 acccgttcgc attggaggaa ttggcataat cgaagatcgc ggcattccca ctcacccagg
     2281 cggaagtcgc gatatcgcca gcggacgaag tcgtccagtt ggtggcgccg ctggcccagt
     2341 tatttgaacc tcccaccgaa tagtcgccga agccgggcgt ggctccgttg aggtcgaaat
     2401 acgtgtcggc ggcctgcgcc agtgaagggc cggcaaacaa agcggcggac gcaaggcggg
     2461 ataaaagatc ccgagccgtt gaggaacttt ttcgagaaga tttgttcatg gggaatgaat
     2521 ggatttgggg tggagtttgg cttccggttg cggagatgtc aatcattaat attggaatct
     2581 tataagattt attttattcg gcgcaaatat aatggaaata tctttggtta aactttttac
     2641 attttatgat tatttgccgg ttgggatcgt gccgggggag cggacggttg cgagccgctt
     2701 tgcttcattg ggtgcaattt cggatcgcct gggcgggagg cggtggattt ttgtccggcg
     2761 tcatgatgcg atgatcgtgg aggcattcgg ggcaaaacct ttcttttttt catcgctgaa
     2821 aaaaagaaag cggcgaagcc atgatttatc atcagcctca actggcggg
//
LOCUS       DATDAY010000378         2104 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_513229, whole genome shotgun sequence.
ACCESSION   DATDAY010000378 DATDAY010000000
VERSION     DATDAY010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2104)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2104)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2104
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_513229"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..237
                     /locus_tag="VIS74_07460"
     CDS             <1..237
                     /locus_tag="VIS74_07460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5793119.1"
                     /translation="LIYGEVPRATNEEAAGYFQKAIALNPGRIGNHVELGRTCLALRD
                     SAKAKAEFAKALALPDREKEDPESKRRARAALAQ"
     gene            517..>2104
                     /locus_tag="VIS74_07465"
     CDS             517..>2104
                     /locus_tag="VIS74_07465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5793120.1"
                     /translation="MNCLSAAANADGSYAVPIYHDLQYGSVHVLKIYAGLNGGPMVPF
                     LLDSGSPNLFAAYGSWWPGSPTTVVKGTHRDKFSSGNEFFYKSAHATVTFGDRRQNAI
                     AGIDAKLAMLTKATVKGETRDYRYWKKVNPFTETVLPNDTFGNMGLGLEGNSAVSGLA
                     TLAAQVPLAKGMQTGFVINTGGSLAARAQLTLGLSPGVVKMFQAPQAIVLQMGATGGT
                     IPGPGGSTVPAYGEAQASATTLTVTNQGRTLTLALPTIFDTGGGNNTYINAAGTILDT
                     GINYPVQGLSVSQGLRQVFDYVTSNPLGGGLYVTTGGGTDPRTNTGLSIFMQYLVLFN
                     LTEGWMSLLPFSALNSVPFLSNDFTPASTAFIVSGPVTTSQSPVKIHSLSFDPGSSLE
                     IANSLTVKSGNFGVANGAAAINGGALLVPGDLTKTGAGALAIASDVRVGGAAMIRAGG
                     LVINQTLTAAQGTRVLPGAWLGGNGVIVGAVTNAGAVNPGNSPGALTIVGDFTQTSGG
                     TLTIEAQSAAVYDRLLITGHA"
BASE COUNT          388 a          688 c          652 g          376 t
ORIGIN      
        1 ctcatctacg gcgaagttcc ccgggcgacc aacgaggagg cggcgggata ttttcagaag
       61 gcgatcgcgc tgaaccccgg tcgcatcggc aaccacgtcg agctgggccg gacctgcctg
      121 gccctacgcg acagcgccaa ggcgaaggcg gagtttgcga aggcgctggc gctcccggat
      181 cgcgaaaagg aggatccgga atccaagcgg cgcgcccgcg cggcgcttgc tcaatgagct
      241 gagcggcagt caaattgaaa gccttttcgg ttcaatggga cgcctttccc cggcggggtt
      301 ggcaaccccg ccccaccgcg aaccgatctt tgggtagggc ggcgttgcca acgccgccgc
      361 tcaccgcgac aaactaaacc gaatgggttc taattaggtt gccatccggt cattttgctt
      421 cctaattggc tccccatgcg aaacgggatt cgggatcggg tgaggggctt aaagtgctta
      481 aggggcttga tgattgcaat ggggctggcg ttctcgatga actgcctttc cgccgccgcc
      541 aatgccgacg gcagttacgc ggtccccatt taccacgatc tccaatacgg ctcggtgcat
      601 gtcctgaaga tttacgccgg cctgaacggg gggccgatgg ttcccttcct cctggattcc
      661 ggctcaccga atttgttcgc cgcctacgga agctggtggc ccggcagtcc cacgaccgtg
      721 gtcaagggca cgcatcgcga caaattttcc agcgggaacg aatttttcta taaatccgcc
      781 cacgccaccg tcacgttcgg cgacaggcgg cagaacgcca tcgccggaat cgacgccaaa
      841 ctcgcgatgt tgaccaaggc caccgtgaaa ggcgaaacgc gggattaccg ttattggaaa
      901 aaggtgaatc cgttcaccga gaccgtcctg cccaacgata ccttcggaaa catgggcctg
      961 ggcctggagg gaaacagcgc cgtgtccgga ctggcgaccc tggccgccca ggttcccctg
     1021 gcgaagggga tgcagaccgg ctttgtgatc aataccgggg gcagccttgc cgcccgcgcc
     1081 caattgactc tcggcctctc gccgggagtg gttaaaatgt tccaagcccc gcaggcgatc
     1141 gttctgcaaa tgggcgcaac cggcggcacg atacccggcc cgggcggctc cacggtcccc
     1201 gcctacgggg aggcccaggc ttccgccacc acgctcaccg tcacgaacca gggccggacc
     1261 ctcacgctcg ccctgccgac gatcttcgac accggcgggg gaaacaatac ctacatcaac
     1321 gccgccggca cgatcctcga caccggcatc aactatccgg tgcaaggcct ctcggtcagc
     1381 caggggctgc ggcaagtctt cgattacgtg accagcaacc ccctgggggg cggcctctat
     1441 gtgaccaccg gcggcggcac ggacccgcgc acgaacaccg ggctctccat tttcatgcag
     1501 tatctcgtgc tctttaatct caccgagggc tggatgtcgc tgctgccctt ttccgcgctc
     1561 aattccgtgc cgtttctctc gaatgatttc acgccggcgt ccaccgcgtt catcgtgagc
     1621 ggtccggtga ccaccagcca gtcaccggtg aagatccatt cgctttcctt cgatcccggc
     1681 tcgtcgctgg aaatcgcgaa ttccctgacc gtgaagagcg ggaatttcgg cgttgcgaac
     1741 ggcgcggccg cgatcaatgg cggcgcgctg ctggttcccg gcgatctgac gaaaaccggc
     1801 gcgggagcgc tcgccatcgc ctccgacgtg cgggtgggcg gagcggccat gattcgagcg
     1861 ggcgggctgg tgatcaatca aacgctcacc gcggcgcagg ggacgcgggt tcttccgggc
     1921 gcctggctgg gagggaatgg cgtgattgtg ggcgccgtca ccaacgcggg cgccgtcaat
     1981 cccggaaact cgccgggcgc cttgacgatc gtgggcgatt ttacccagac cagcggcggc
     2041 acgctgacga tcgaggcgca gagcgcggcc gtttacgacc gcctgctcat taccggccac
     2101 gcca
//
LOCUS       DATDAY010000379         3062 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_524592, whole genome shotgun sequence.
ACCESSION   DATDAY010000379 DATDAY010000000
VERSION     DATDAY010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 3062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3062
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_524592"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1916
                     /locus_tag="VIS74_07470"
     CDS             <1..1916
                     /locus_tag="VIS74_07470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5793121.1"
                     /translation="LQTLLKTLSSRLAVNQSKASMQARIEQMILRQVANLRQTQDLAA
                     AGKKAPAALASVCANEQDSLKQETQLVLEALQNVINGMPAAERPGFAESLQEARRLDL
                     GALAATAASETTAAQFTQAAGSQGKFLGGLQTLLATLTAGQTAQERAQALAAKLQDLA
                     GKQKQLAETSKTADGYRQRELRDAQDRLSDELNAIQNQIQALNANASAQTHDAAADMK
                     EANQALKNSRFDKDTKAALAAAQTAAAEKLRTASENLQNQASALAQNAAPQNAAQAMN
                     ALGQLAQQIAQAQANQNALNNSPNASAAQMQQQASQTAALQQAAAPLNAQAAASLGQA
                     AASMQQAASQAGASPSNASASPQSAPQSASQALASASAAVQQQMQALAQAAAAQQALQ
                     SLMNQVGAAASQTSQAGQSLKNTPQSGDLTAAIKQMDTAQQLLQQAQASAGSSAVPPE
                     AKASLQTAQAALSASKLNAAQMKRGEAQAANAQAQKALQGAMASLQQAAAGAMAQAMP
                     PGSQSGSSAGQQASNPPGGQPPGGQPGGSPQGGQPAGGQPAGGPPMAGSGGPAGGPSG
                     EGSGMGGQVGSFMNGSGAQGGPAGVVTGLKPKDRAAIAALRKEKAPAEYQGMAQQYLK
                     NLAEGVAPGSVVE"
     gene            1913..2233
                     /locus_tag="VIS74_07475"
     CDS             1913..2233
                     /locus_tag="VIS74_07475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5793122.1"
                     /translation="MIPHGLAVLAGAAVFSHSLPARAEAPPPVRLPVVKTWGELKAAP
                     PVIRESLATIAHGDGNGPPPTIPSRCSKCPSLSGKDQPIPAPSRLFARVNYQWNGKSL
                     IPEK"
     gene            2247..>3062
                     /locus_tag="VIS74_07480"
     CDS             2247..>3062
                     /locus_tag="VIS74_07480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5793123.1"
                     /translation="MIIPCLSTFACADLTLRFPWDPATGRVGFEILPTALAGRTQPRR
                     DGLAGLPYIDSQPGAGNPPAYVVDPLAPVKILGDPYSGGFAQGHTLRNSPSIEAFSYE
                     GQEVARNGGQTTVVTTLRHAAGHRLAHRASWRDGEAAVEIRTTFFNGSPRPVTLEMLG
                     SFSLGGISPFDPADNAGRLIVHRFRSGWSAEGRLETRSIEELHLERSWHGGALFSERF
                     GQAGSMPVRKWFPFAAIEDTAAGVVWGAQLAWAGSWQLEIFRQHDDVCLSGGLA"
BASE COUNT          569 a         1164 c          949 g          380 t
ORIGIN      
        1 cgcttcaaac cctgctcaag accctctcca gccgcctcgc cgtcaaccaa agcaaggcct
       61 ccatgcaggc gcgcatcgag caaatgatcc tgcgccaggt ggccaatctg cggcaaacgc
      121 aggatctcgc cgccgccggc aaaaaggccc ccgccgccct cgcctcggtc tgcgccaatg
      181 aacaagactc gctgaagcag gaaacccagc tcgtcctcga agcgctccag aacgtcatca
      241 acggcatgcc cgccgcggag cggccgggat tcgccgaatc gctccaggaa gccaggcgcc
      301 tcgatctcgg cgcgctcgcc gcgacggccg ccagcgaaac caccgccgcc caattcaccc
      361 aggccgccgg ttcgcagggc aaattcctgg gcggcctgca aaccctcctc gccaccctca
      421 ccgccgggca aaccgcccag gaacgcgccc aggcccttgc cgccaaactt caagatctgg
      481 ccggcaagca aaaacagctc gccgaaacgt ccaagaccgc ggatggctac cggcagcgcg
      541 aactccgcga cgcgcaagac cgcctttccg atgaactgaa cgcgatccag aatcaaatcc
      601 aggccctcaa cgccaacgcc tccgctcaga cccacgacgc ggcggcggac atgaaggaag
      661 ccaaccaggc cctcaaaaat tcccgcttcg acaaggacac caaagccgcc ctggcggccg
      721 cccagacggc ggcggcggag aaattgagaa ccgccagcga gaatctccaa aatcaagcct
      781 ccgcgctcgc ccagaacgcc gcgccgcaaa acgccgccca agccatgaac gcgctgggcc
      841 agctcgccca gcaaatcgcg caggcccagg caaaccagaa tgcgctgaac aactccccca
      901 acgccagcgc cgcgcagatg cagcagcagg ccagccaaac cgccgccctg cagcaggccg
      961 ccgcgccgct caacgcccag gccgccgcca gcctgggaca ggccgccgcc agcatgcagc
     1021 aggccgccag ccaggcgggc gcttccccga gcaatgcctc cgccagcccc cagagcgcgc
     1081 cgcaaagcgc ctcccaggcg ctggctagcg ccagcgcggc cgtccagcag caaatgcagg
     1141 ccctcgccca ggcggccgcc gcgcagcagg cgctgcagtc cctgatgaac caggtgggcg
     1201 ccgccgcgag ccagacgagc caggcgggac agtccttgaa gaacaccccc cagagcgggg
     1261 atctgacggc cgccatcaaa caaatggaca ccgcccagca attgctccag caagcccagg
     1321 catccgccgg ctcatcggcc gtcccaccgg aggccaaggc cagcctccaa accgcccagg
     1381 ccgccctttc cgcctcgaaa ctgaacgccg cgcagatgaa gcgcggggaa gcccaggcgg
     1441 ccaacgccca ggcccagaag gcgctgcaag gcgccatggc cagcctccag caggccgccg
     1501 ccggcgccat ggcccaggcc atgccccccg gctcgcagag cggctccagc gccggccagc
     1561 aggccagcaa cccacccggc ggccagcctc cgggaggcca gccgggcggc agcccgcagg
     1621 gcggccaacc ggcgggggga cagcccgccg gcggacctcc gatggccggt tccggcggcc
     1681 cggcaggcgg gccctccggc gaaggcagcg gcatgggcgg ccaggtcggc agttttatga
     1741 acggttccgg cgcccagggc ggacccgccg gggtcgtcac cggcctcaaa cccaaggatc
     1801 gcgccgcgat cgccgccctg cggaaggaaa aggcccccgc cgaataccag ggcatggccc
     1861 agcaatattt gaaaaatctc gcggaaggcg tcgctcccgg cagcgtggtg gaatgatccc
     1921 gcacggcctg gcggttctgg cgggcgccgc ggtcttttcc cattcgcttc cggctcgcgc
     1981 cgaggctccg ccgcccgtcc gtcttcccgt cgtcaaaacc tggggagaat tgaaagcggc
     2041 gccgccggta atccgcgaaa gcctggcgac tattgcccat ggggatggaa atggtccacc
     2101 cccgacgatc ccgtcgcgat gcagcaaatg cccgtccctt tcgggcaaag accagccgat
     2161 ccccgcgccg tcccggctct ttgcccgcgt gaactatcaa tggaacggaa agtccctgat
     2221 tccggaaaaa taaggagcgc ttttcggtga tcattccctg cctttccacg tttgcctgcg
     2281 ccgatctcac gctgcggttt ccctgggacc cggcgaccgg ccgcgtgggt ttcgagatcc
     2341 ttcccacggc gctggccggc cggacgcagc cccgccgcga cggcctcgcc gggttgcctt
     2401 acattgactc ccagcccggc gcgggaaatc cgccggccta cgtggtggac ccgctcgcgc
     2461 cggtaaaaat cctcggcgat ccctactccg gcggcttcgc gcagggccac accctgcgca
     2521 attcgccatc catagaggcg ttttcctacg agggccagga agtcgcgcgg aacggcggac
     2581 aaaccacggt cgtcaccacg ttgaggcatg ccgcggggca tcgcctggcc caccgcgcct
     2641 cgtggcggga cggcgaggcg gcggtggaga tccgcaccac cttttttaac ggctcgccgc
     2701 ggccggtcac gctggagatg ctcggcagtt tttcactggg cggcatcagc cccttcgatc
     2761 cggcggacaa cgcgggccgc ctgatagtcc atcgcttccg gtccggctgg agcgctgagg
     2821 ggcggctgga aacgcggagc atcgaggagc tgcatctgga gcgttcctgg cacggcgggg
     2881 ctctgttcag cgagcgcttt ggccaggccg gctccatgcc cgtgcggaaa tggttcccct
     2941 tcgcggcgat cgaggacacc gccgccgggg tggtctgggg cgcgcagctc gcctgggccg
     3001 gctcgtggca gctggaaatt ttccgccagc acgacgacgt ctgcctctcc ggcgggctgg
     3061 cc
//
LOCUS       DATDAY010000380         2207 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_2564309, whole genome shotgun sequence.
ACCESSION   DATDAY010000380 DATDAY010000000
VERSION     DATDAY010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2207)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2207)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2207
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2564309"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1086
                     /gene="metE"
                     /locus_tag="VIS74_07485"
     CDS             <1..1086
                     /gene="metE"
                     /locus_tag="VIS74_07485"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HEY5793124.1"
                     /translation="KIQPSDSRRHSPFALRQPRQHAALKLPILPTTTIGSFPQTGEVR
                     AARAKWKKGEFDDAGYEKFLESEIARAVRRQEEIGLDVLVHGEFERTDMVEYFGEQLA
                     GFAFSQNGWVQSYGTRCVKPPILFGDVSRPQPMTVRWSRFAQSLTALPMKGMLTGPIT
                     ILQWSFVRDDQPRRETARQIALALRDEVMDLEAAGLRVIQIDEPALREGLPLRRSDWA
                     DYLDWAVEAFRLSAAGVRDETQIHTHMCYCEFNDIVESIAELDADAISIEASRSNMEL
                     LAAFRDFAYPNEIGPGVWDIHSPRVPGVEEMTRLLQKAADVIPIRNLWANPDCGLKTR
                     AWEETTAALRNLVAAAKFLRAEFEKRE"
     gene            1096..2202
                     /locus_tag="VIS74_07490"
     CDS             1096..2202
                     /locus_tag="VIS74_07490"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR00045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEY5793125.1"
                     /translation="MRFLIAPDKFKGSLSAEEAAGAIRAGFQRVFPAAEYELAPIADG
                     GEGTAAAFHRTLGGEWIFHPAEDARGRRIEAEFLWLPERRTAVIDMSAASGLARLAEA
                     GRRPWTASTRGTGQLLAEALRRGARTVYLGLGGSATNDAGAGLAAALGWTFRDAEGRA
                     VHPIPQNFRAIRSIHPPASPISAEVIGMTDVANPLLGPAGCSAVFAPQKGARGEEIPE
                     WDAVLRHLSDLVENGARLRELPGAGAAGGLGFGVLAFLGGRLEPGFAVVARLLDLATR
                     AAAADWILTGEGKLDAQTLGGKGPAGVAGLARAAGKPVIAFAGAVDDAIQPGIFTQIV
                     SLTDGQISAAESMARAAELLTAAAERTARSLRAK"
BASE COUNT          378 a          720 c          749 g          360 t
ORIGIN      
        1 aaaattcaac cgtccgacag ccggcggcat tcgccctttg cgctccggca accgcggcag
       61 catgcggcgc tgaagctgcc gattctcccc accacgacga tcggttcatt tccgcagacc
      121 ggcgaggtcc gcgccgcccg ggccaaatgg aaaaagggcg agttcgacga cgctggctac
      181 gaaaaattcc tggaatcaga aatcgcccga gccgtgcgcc ggcaggagga aatcgggctc
      241 gacgtattgg ttcatggtga atttgagcgg accgacatgg tggaatactt cggcgagcag
      301 ctcgcgggat ttgccttctc gcaaaacggc tgggtgcaaa gctacggaac gcgctgcgtg
      361 aaaccgccga ttctcttcgg cgacgtatcc cgcccgcagc ccatgacggt ccgctggtcg
      421 cgcttcgcgc aatcattgac cgccttgccc atgaagggaa tgctcaccgg tccgatcacc
      481 attctgcaat ggagttttgt gcgcgacgat cagccgcgcc gcgaaacggc tcgtcaaatc
      541 gccctcgcgc tgcgcgatga agtcatggac ctcgaagccg ccggcctgcg ggtgatccaa
      601 attgacgaac ccgccctccg ggagggactg ccgctgcggc ggagtgattg ggcggactac
      661 ctcgactggg ccgtggaggc gttccgcctg tccgcagcgg gcgtgcggga cgaaacgcag
      721 attcacacgc acatgtgcta ttgcgaattc aacgacatcg tcgaatccat cgccgagctg
      781 gacgccgacg ccatttcgat cgaggcctcg cgctcgaaca tggaattgct cgccgcattc
      841 cgcgactttg cgtatcccaa cgagatcggg ccgggcgtct gggacatcca ttccccgcgc
      901 gtgcccggcg tggaggaaat gacgcgcctg ctgcaaaagg cggcggacgt cattccgatt
      961 cgcaatctct gggcgaatcc ggactgcggt ttgaaaacgc gggcctggga ggaaaccacg
     1021 gcggctctcc ggaatctggt ggccgccgca aaattccttc gcgcggaatt cgaaaagcgg
     1081 gaatgatttt ccgcgatgcg ctttctcatc gccccggaca aattcaaggg ttcgctttcc
     1141 gccgaagagg cggccggggc cattcgcgcc ggttttcaac gggttttccc cgcggcggaa
     1201 tatgagctgg cgcccatcgc ggacgggggc gaaggcacgg cggccgcctt ccatcgcacc
     1261 ctgggcggcg aatggatttt ccaccccgcc gaagacgcgc gggggcgccg gatcgaagcc
     1321 gaatttcttt ggctcccgga gcgccggacg gcggtcatcg acatgagcgc cgcctcgggg
     1381 ctggcgcggc tggccgaagc cgggcgccgg ccctggaccg cctccacgcg cggcaccggg
     1441 cagcttctgg cggaagcgct gcggcggggc gcgcggacgg tttatctcgg cctcggcgga
     1501 agcgcaacca atgacgccgg agccggcctg gcggcggcgc tcggctggac attccgcgac
     1561 gccgaggggc gggccgtcca ccccattccc caaaactttc gcgccattcg cagcatccat
     1621 ccgccagcat cgccgatttc ggcggaggtc atcggcatga ccgatgtcgc caatcccctg
     1681 ctcgggccgg ccggatgcag cgcggtattc gcgccgcaga agggagcccg gggcgaggaa
     1741 attcccgaat gggacgcggt cctccggcac ctttccgatc tcgtggagaa cggcgcccgg
     1801 ctccgcgagc tgcccggagc gggcgcggca ggcgggctgg ggtttggcgt cctggccttt
     1861 ctcggcgggc gcctggagcc gggattcgcg gtcgtcgcgc ggctgctcga cctggctacg
     1921 cgcgcggccg cggcggattg gatcctcacg ggcgaaggca agctggacgc gcagaccctg
     1981 ggcggcaagg ggccagccgg cgtggccgga ctggcccgcg cggcggggaa accggtgatc
     2041 gcgtttgccg gagccgtgga cgatgccatc cagccgggaa tcttcaccca aattgtctcc
     2101 ctgaccgatg gacaaatttc cgcggcggaa tccatggcgc gggcggcgga gttattgacc
     2161 gcggccgccg aacgcacggc gcggtcgctg cgagcgaaat gaatccg
//
LOCUS       DATDAY010000381         2626 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_1727791, whole genome shotgun sequence.
ACCESSION   DATDAY010000381 DATDAY010000000
VERSION     DATDAY010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2626)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2626)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2626
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1727791"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..834
                     /locus_tag="VIS74_07495"
     CDS             <1..834
                     /locus_tag="VIS74_07495"
                     /inference="COORDINATES: protein motif:HMM:NF014471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HEY5793126.1"
                     /translation="AKKALTSAGFAAIALAAFVAIFAFKVSFLLILLAAALAGVILGR
                     PASPHPAPPLEERPAHSARVIAGGLLLWWAPILLAGLIFGWGSLYFQQGLFFSKTALI
                     TFGGAYAVLPYVAQQAVEQFGWLQTNQMIAGLALAETTPGPLIMVLQFIGFLAAWQQP
                     GGLPPLLAGTLGAAITTWATFLPSFLLVFLGAPFVERLRQAPRLKAALAGITAAVVGV
                     ILNLAVWFGWHIAFPGGRGFDAAALIFSGLFFAGIQWRKWSTPTVVAAGALIGLIRFW
                     I"
     gene            complement(845..1276)
                     /locus_tag="VIS74_07500"
     CDS             complement(845..1276)
                     /locus_tag="VIS74_07500"
                     /inference="COORDINATES: protein motif:HMM:TIGR00028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system VapC family ribonuclease toxin"
                     /protein_id="HEY5793127.1"
                     /translation="MTPRFLPDANIYLNALHSRSAHHAECRNWLDQTTAARHRLLLND
                     LTECAFLRISTLPRLNFASLGSALEFWDRLLDYSGMERATPGPAHAHLFRRLLKDLGL
                     AGNEINDVWLAALCLENDATLVSTDQGFSRFPGLKWLNPAQ"
     gene            complement(1273..1545)
                     /locus_tag="VIS74_07505"
     CDS             complement(1273..1545)
                     /locus_tag="VIS74_07505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014588668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5793128.1"
                     /translation="MQTTLRLDDQLYREAKAEAAREGTTLTRFLEDAIRLRLRKKSSP
                     APGEPHPFPLYEGGAAILPSDETIKEIAVEEEERYLLKKFIGGSTR"
     gene            1827..>2626
                     /locus_tag="VIS74_07510"
     CDS             1827..>2626
                     /locus_tag="VIS74_07510"
                     /inference="COORDINATES: protein motif:HMM:NF017817.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY5793129.1"
                     /translation="MSNPYSSNPDVVLVGAGIMSATLAVLLKELNPNLTIEIFESLNR
                     ISRESSNAWNNAGTGHAALCELNYTPEKPDGSIPIDKAAEINEAFEISKQFWAYLVRK
                     GALANPAEFINQVPHLSFVHGEKNVDFLKRRHAALRGHPFFAGMEYSDDRAKLAEWMP
                     VMMAGRADGEPLAATRMEIGADVNFGTLTSRLIAYLQKQEGVFLHLRHRIVDLKRRRN
                     GEWTVVAHDRMNRKRTMSAKFVFLGAGGGALPLLQKSGIREGKGYGGFP"
BASE COUNT          506 a          797 c          808 g          515 t
ORIGIN      
        1 gccaaaaagg cgctcacgtc agcggggttc gccgcgatcg ccctggcggc cttcgtggcg
       61 atttttgcgt tcaaggtttc gttcctcctc attctgctgg ccgccgcgct ggccggcgtg
      121 atcctcggac gtccggcctc cccccatccc gcgccgccgc tggaggaaag gcccgcgcat
      181 tccgcacgcg tgatcgccgg cgggctgctg ctgtggtggg cgcccattct gctcgcgggg
      241 ctgatcttcg gctggggcag cctctatttt cagcagggac tctttttcag caaaaccgcg
      301 ctgatcacct tcggcggcgc ctacgcggtg ctgccctacg tagcgcagca ggcggtggag
      361 caattcggct ggctgcaaac gaatcaaatg atcgccgggt tggcgctcgc ggaaaccacg
      421 ccgggaccgc tcatcatggt cctgcaattc atcggctttc ttgccgcgtg gcagcagccg
      481 ggcgggctgc ctcccctgct tgcgggaacg ctcggcgcgg ccatcaccac ctgggcaacg
      541 ttcctgccca gcttcctgct cgtgttcctc ggcgcgccct tcgtggaacg cctgcgccag
      601 gcgccgcgcc tgaaagccgc gctggcgggc atcaccgccg cggtggtcgg cgtgatcctg
      661 aacctcgccg tgtggttcgg ctggcacatc gcgtttcccg gcgggcgggg attcgacgcc
      721 gcggcgctga ttttcagcgg gctgttcttc gccgggattc aatggagaaa atggagcacg
      781 ccaaccgtcg tggccgccgg ggcgctgatt ggattaatcc ggttttggat ataaccgcga
      841 aatcttattg ggccggattc agccatttca gaccgggaaa gcgggaaaat ccctgatcgg
      901 tggaaaccag cgtggcatcg ttctccaaac agagggcggc gagccagacg tcgttgatct
      961 cattgccggc caagcccaaa tccttcagaa ggcgacggaa aaggtgagcg tgggcggggc
     1021 cgggagtggc tcgctccatg ccggaataat cgagaagccg atcccaaaat tccaacgccg
     1081 atcctaggga ggcgaagttc aagcgcggca gggtgctgat ccgaagaaag gcgcattcgg
     1141 tcaagtcatt caacaacagc cgatggcgcg ccgccgtggt ttgatccaac cagttccgac
     1201 actcggcgtg atgcgccgag cgcgaatgaa gggcgttcaa gtaaatgttc gcgtctggca
     1261 gaaaacgcgg ggtcatcgag tagacccgcc aatgaatttt ttcaaaagat agcgttcttc
     1321 ctcttcgacg gcgatttcct tgatcgtttc atcggatgga aggatggcgg cacccccttc
     1381 gtagagggga aatggatgag gttcgcccgg cgccggggag gattttttgc gcaaacggag
     1441 acggatcgca tcctccaaaa agcgggtcaa ggtcgttccc tcccgggccg cctcggcctt
     1501 ggcttcacga tagagctgat catcaagccg caaggtcgtt tgcatgaatg aaatctttca
     1561 taagaaagaa atctgtcaaa tgttaaatat tcagatcaga ttccgcagag agatccagaa
     1621 aacgcggagt gaaggggaaa aacatccatc atttacacag atttgcgccg actgggctct
     1681 tatccatgcc catccggctc atttgcggct aactcggtcg gccttcggct cttcgccctt
     1741 gccccgattt cgaaacaaaa acttgatcgc tccgggtttc cggcgtggat tagtgcgctt
     1801 gacgggcgcg cggcgtcctt ccctttatga gcaatcccta ttcttccaat cccgatgtgg
     1861 tcctcgtcgg cgcgggcatc atgagcgcca ccctggcggt tctcctcaag gagctgaacc
     1921 cgaatctgac catcgaaatc ttcgaaagcc tcaaccgcat ttcccgggaa agctcgaacg
     1981 cctggaacaa tgccggcacc ggccacgcgg cgttgtgcga actgaattac acgccggaaa
     2041 agccggatgg ctccatcccc atcgacaagg cggcggagat caatgaagcc ttcgagattt
     2101 ccaagcagtt ctgggcgtat ctcgtgcgca agggggcgct cgccaatccc gccgaattca
     2161 tcaaccaagt cccgcacctg agcttcgtgc atggggaaaa gaacgtggat ttcctcaagc
     2221 ggcgccacgc cgcgctgcgc gggcacccgt ttttcgcggg gatggaatat tccgatgacc
     2281 gcgcgaaatt ggcggaatgg atgccggtca tgatggccgg ccgcgcggac ggcgaacccc
     2341 tggcggcgac gcgcatggag atcggcgcgg acgtgaattt cggcacgctc acgagccggc
     2401 tcatcgcgta tctgcaaaag caggaaggcg tgttcctgca cctgcgccac cgcatcgtgg
     2461 acctcaagcg ccggcgcaat ggcgaatgga cggtcgtcgc gcacgaccgc atgaaccgca
     2521 agcgcacgat gagcgcgaag tttgtctttc tcggcgcggg cggcggggcg ctgcccctgc
     2581 tgcagaagtc cggcattcgc gaaggcaagg gctacggcgg cttccc
//
LOCUS       DATDAY010000382         5738 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_1909047, whole genome shotgun sequence.
ACCESSION   DATDAY010000382 DATDAY010000000
VERSION     DATDAY010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 5738)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5738)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5738
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1909047"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            86..2296
                     /gene="priA"
                     /locus_tag="VIS74_07515"
     CDS             86..2296
                     /gene="priA"
                     /locus_tag="VIS74_07515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEY5793130.1"
                     /translation="MAVFARVLVDRSAGRLLDYQIPSALESVVAAGSRVRVPVRQRLL
                     LGTVVELRDSSDARGLREIHEALSDRAMIRPVILEMVRWMADYYCCSAEAASRTVLPQ
                     VVREAQIGPRELNAVEPAREISDEELEKMEVRSPRRVELLRRVRAAGAPVRVQELLRE
                     TGASSAVVQALVRGGWLKLRTVQLGRDPHGGEVFLGTEALALTAQQRAALEAVSAASR
                     PVLLHGVTGSGKTEIYLQAIERTLAQGKQALVLVPEISLTPQTVERFKSRFSSFQKEV
                     AVMHSHLSEGERYDEWHKIHHGQARIVIGARSAVFAPLERLGLIVVDEEHEPSYKQEE
                     APKYHARDLAVLRAKLEGCAIVLGSATPSLESYHNARMGKYELVRLTERIDGRKLPII
                     RVLDMRVQRRRLGNAESLLAAPLCQAIEERLAKREQTILFLNRRGYSTSLLCGECGHV
                     CECPNCSIALTFHRDTEKVACHICGHVAVAPHRCPECQTPGIRYAGAGTQKVEESVRK
                     LFPKARIARMDADTMTRKDAYRETLGAFRAGQIDLLVGTQMIAKGLDFPNVTLVGIIN
                     ADLSLHLPDFRAGERTFQLLVQVAGRAGRGEMEGEVYVQTHTPFSPSIQFARQHDYEG
                     FWEQEADFRQRFGYPPFERLLLLTVRSEKRELAEFTAQTLGRRLAEKLPEGMRLGEPV
                     PAPLEKAKGAYRFHLSLRGASALKLSRHARSVLDALTLPAEVFISPDVDPQNLL"
     gene            2333..4750
                     /locus_tag="VIS74_07520"
     CDS             2333..4750
                     /locus_tag="VIS74_07520"
                     /inference="COORDINATES: protein motif:HMM:NF024893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HEY5793131.1"
                     /translation="MSLKSATSFLLRLAIRAVLLLLFVGLPAGLFYLNGVGVTGPWTR
                     EISRALSSANFQVQVGRLTLNPFEGISAHDLEIYNAGGLRLAKVSRVLVGLNLSELIR
                     RRVQIETMELVRADLSIPASGGPPLAVTNVRGHLRVSPTQVSVTEFGFKLAGIQFFVH
                     GTMRRDRQPAARPWSNLPVARPGGAPNPLVEQIRRVAQEISFGATSPQIHLELAGSGS
                     SPDEIEAPRILAEFPEIRWKNLQFKQARLEAAYAGGIFTVKSLSAEDAHGQINTAFTV
                     DLRQKILRADLLSSIDPAPLLAAFSQSEWSREIVFHQPPRLNLELTLDYDKPSPEWLV
                     NGGIDIENFRVKGVPFDRVNGDFTYRQGQFLTRDLNLQAPNLDLRMDLMADARTARVR
                     VDGSVDPLPLQPLLGAGLGVIFGQMQFAERGQVRFEGEIPRANPGNLTGSGTLRLGKT
                     AMRGAWIDSGKAEVEMKDRAVFYRHLEVRRGKGVGTGNFIYDFGGKQVRLENIRSTLN
                     PQEIMLWVDAGIAKTVAAYRFKANPAATADGTVDMAHPERSTLDISVQAPAGLDYDLL
                     GKTLSFGATNGALRLRGPMIYADIKSARLFGGTVALTAEVPVTRDLKKYRVSVQANHV
                     NFPALTRLYFNYANSEGWMSGRFSFEAPLADSRSLAGKGNIRIEEGHVFGIPIFGPFS
                     GILNGIIAGAGYQTARKATADFTIADQIIHTGNLEIQGQGFSMFGEGDIHFVTDKMDM
                     SMRINAQGVPGIVLFPVSKLFEYVSYGTISDPVWRPKNVPRQLFGDPAPKEEANRREP
                     ANKPPGR"
     gene            4987..5577
                     /locus_tag="VIS74_07525"
     CDS             4987..5577
                     /locus_tag="VIS74_07525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5793132.1"
                     /translation="MSRSYRSQKESVTAERRIERTRDGSPRLPHIIERAPAPGDSHPL
                     PKRVLHRFLQRVPVEFIYGLSRIELRARELSLIGAPFGSYWVGERAIVLYSLPPVFHC
                     RSLSSDFRGSLKRFHATIQSDANGVTVSWREPAVMSMWFYSFVFTHELGHHFVEQYKS
                     KNSRIHSRNAEELVADLHAKRFTDELFASFRKRRSI"
BASE COUNT         1035 a         1786 c         1847 g         1070 t
ORIGIN      
        1 ccatgacgcg gaaattatgc gaccgaaatc cgcggaggcg agcgattttc tttccctgca
       61 agcccggcgc tggaaataat ccggcatggc tgtcttcgcg cgtgttttgg tggatcgttc
      121 cgccggccgg ctgctcgact atcagatccc ctccgcgctg gaatccgtcg tggcggccgg
      181 atcgcgcgtg cgcgtgccgg tgcggcagcg cctgctgctg gggacggtcg tcgagctgcg
      241 ggactccagc gatgcgcgcg ggctgcggga aatccacgag gcgctgagcg accgggcgat
      301 gatccggccc gtgatcctcg aaatggtgcg ctggatggcg gattattatt gctgctccgc
      361 cgaggcggcg agtcgcacgg tgctgccgca ggtggtccgg gaggcgcaga tcgggccgcg
      421 ggagttgaac gccgtcgaac ccgcgcggga aatttccgac gaggaattgg aaaaaatgga
      481 ggtccgctcc ccgcgccgcg tggagttgct gcgccgcgtg cgcgccgcgg gcgcgccggt
      541 gcgggtccag gagctgctgc gggaaaccgg cgcgtcctcc gcggtggtgc aggcgctggt
      601 ccggggggga tggctgaagc tgcgcacggt tcagttgggc cgggatccgc acggcgggga
      661 ggttttcctg ggcaccgagg ctctggcgct caccgcgcag cagcgggccg cgctggaagc
      721 ggtttccgcg gcgtcccggc ccgtgctgct tcacggagtg acgggcagcg ggaagacgga
      781 gatttatctg caagccatcg aacgcaccct ggcgcaagga aaacaggcgc tcgtgcttgt
      841 tcccgaaatc tcgctgacgc cccagacggt ggaacgcttc aagtcgcgct tttcctcgtt
      901 ccagaaggag gtcgcggtca tgcatagcca tttgtccgag ggggagcgtt acgacgaatg
      961 gcacaagatc caccacggcc aggcgcggat cgtcatcggc gcccggagcg cggtgtttgc
     1021 gccgctggag cggctgggat tgatcgtcgt ggacgaggag catgagccct cctacaagca
     1081 ggaggaggcg ccgaaatacc acgcgcggga tctcgccgtg ctccgggcga aactcgaggg
     1141 ctgcgcgatc gtgctcggca gcgccacgcc ctcgctcgag agttaccaca atgcccgcat
     1201 gggtaaatac gagctcgtcc ggctgaccga gcggatcgat ggccggaagc tgcccatcat
     1261 tcgcgtgctc gacatgcggg tgcagcggcg ccggctgggc aacgcggagt cgctgctggc
     1321 cgcgccactc tgccaggcga tcgaggagcg gctcgccaag cgcgagcaga ccattctgtt
     1381 cctgaaccgg cggggctatt ccacctcgct cctctgcggc gaatgcggcc atgtctgcga
     1441 gtgcccgaac tgcagcatcg cgctgacctt ccatcgcgac acggagaagg tggcctgcca
     1501 catctgcggg cacgtcgcgg tggcgccgca ccgctgcccg gaatgccaga cgccgggaat
     1561 ccgctacgcc ggcgcgggca cccagaaggt ggaggaatcc gtgcggaagc tttttcccaa
     1621 ggcgcggatc gcgcgcatgg atgcggatac gatgacgcgc aaggacgcgt atcgcgaaac
     1681 gctgggggcg ttccgggccg gccagatcga ccttctagtc ggcacgcaga tgatcgccaa
     1741 ggggctggat tttccgaacg tgacgctggt cggcatcatc aatgccgacc tcagcctgca
     1801 tctgcccgac ttccgcgccg gcgagcggac gtttcaattg ctcgttcagg tggcgggccg
     1861 cgccggccgg ggggaaatgg agggggaggt ttacgtgcag acgcacacgc ccttcagccc
     1921 gtccatccaa tttgcccggc agcatgatta cgagggcttc tgggagcagg aggccgattt
     1981 ccgccagcgg tttgggtatc cgcccttcga gcgcctgctg ctgctcacgg tgcggagcga
     2041 aaagcgggag cttgccgaat tcaccgccca aacgctgggg cgccggctcg cggaaaaatt
     2101 gccggaggga atgcgcctgg gcgagcccgt gcccgcgccg ctggagaagg ccaagggcgc
     2161 gtatcgcttc catctttccc tgcgcggggc cagcgcgctg aagctttccc gccacgcccg
     2221 gagcgtgctg gatgcgctaa cgctgccggc ggaggttttc atcagcccgg atgtggatcc
     2281 gcagaatttg ctgtagattc cgggcagtca tgagggctag gttgaaactc ccgtgagttt
     2341 gaaatcggcc acttcctttc tgcttcgcct cgccatccgc gctgtgctgc tgctgctgtt
     2401 tgtgggattg ccggccggct tgttttacct caatggcgtg ggcgtcaccg gaccatggac
     2461 gcgggaaatt tcccgcgccc tgagctcggc gaatttccag gtgcaggtcg gccggctgac
     2521 gctgaatccc ttcgagggaa tttccgcgca cgatctggag atttacaatg ccggcgggct
     2581 ccggctggcg aaagtcagcc gcgtgctcgt cgggttgaat ctcagcgaac tcatccggag
     2641 gcgcgtccag atcgagacca tggagctggt gcgggcggat ctttccatcc ccgcttccgg
     2701 cggcccgccg ctcgcggtca ccaacgtgcg ggggcacctg cgggtttccc ccacgcaggt
     2761 cagcgtgacg gaattcggct tcaagctggc cggcattcaa tttttcgttc acggcacgat
     2821 gcggcgcgac cgccagccgg ccgcgcgccc ctggtcgaat cttcccgtcg cccggcccgg
     2881 cggagcgccg aacccgctcg tggagcaaat ccgccgggtg gcccaggaaa tttcgtttgg
     2941 cgccacttcc ccgcagattc acctcgagct ggcgggttcg ggcagctcgc cggacgaaat
     3001 cgaggcgccg cgcatcctgg cggaatttcc cgagattcgc tggaaaaacc tccagttcaa
     3061 gcaggcgcgc ctggaggcgg cctacgccgg cggcatattc accgtcaaat cgctttcggc
     3121 ggaggacgcc catggccaga tcaacaccgc cttcaccgtg gatcttcgcc aaaaaatcct
     3181 gcgggcggat ctactgtcgt ccattgatcc ggcgcccctc ctggcggcat tttcccaatc
     3241 ggaatggagc cgcgaaatcg tcttccacca gccgccgcgc ctgaatctcg aattgactct
     3301 ggactacgac aaaccctcgc cggaatggct cgtgaatggc ggcattgaca tcgaaaactt
     3361 ccgggtgaag ggcgtgccgt ttgatcgggt gaacggggat tttacctatc gccagggcca
     3421 gtttctgacg cgggatctga acctgcaggc gccgaacctg gatttgcgga tggacctgat
     3481 ggccgatgcg cggacggcca gggtccgggt cgatggctcg gtggacccgc tgccgctgca
     3541 acccctcctg ggcgcgggcc tgggggtgat cttcgggcag atgcagtttg ccgagcgcgg
     3601 ccaggtgcgg ttcgagggcg aaatcccgcg ggcgaatccg ggaaatctca ccggctccgg
     3661 aaccctccgc ctcggcaaga cggccatgcg cggggcgtgg attgattccg gaaaggcgga
     3721 ggtcgaaatg aaggatcgcg ccgtttttta ccgccacctc gaagtccgcc gcggcaaggg
     3781 ggtgggcacg gggaatttca tctacgactt cggcggcaag caggtgcgcc tggaaaacat
     3841 ccgttccacg ctcaacccgc aggaaatcat gctctgggtg gatgccggca ttgcgaaaac
     3901 cgtggcggcc tatcgcttca aggccaatcc cgccgcgacc gccgacggca cggtggacat
     3961 ggcccacccg gagcgaagca cgctggacat ctccgttcag gccccggcgg gcctggatta
     4021 cgatctgctg ggcaaaacgc tgagctttgg cgccacaaac ggggcgctgc gcctgcgcgg
     4081 gccgatgatt tacgccgata tcaaatccgc gcggctcttc ggcggcacgg tggcgctgac
     4141 cgccgaggtt cccgtgaccc gcgacctcaa gaaataccgg gtctcggtgc aggcgaacca
     4201 cgtgaacttc cccgcgctga cgcggctgta ttttaattac gccaactccg aaggctggat
     4261 gagcggccgt ttcagcttcg aagcgcccct ggccgacagc cggagcctcg cgggcaaggg
     4321 gaatatccgc atcgaggaag gccatgtgtt tggcatcccg atcttcggac ccttttccgg
     4381 catcctgaat ggcatcatcg ccggcgcggg ctaccagacc gcccggaagg ccacggcgga
     4441 ttttacgatc gcggatcaaa tcatccacac cggcaacctg gagattcagg gccagggttt
     4501 cagcatgttt ggcgagggcg acatccattt tgtgaccgac aagatggaca tgagcatgcg
     4561 gatcaacgcg cagggggtgc ctggcatcgt gctttttccg gtcagcaagc tgtttgaata
     4621 cgtttcttat ggaacgattt ccgatcccgt ctggcggccg aaaaacgtgc cccgccagct
     4681 cttcggcgac ccggcgccca aggaggaagc caatcggagg gaaccggcga acaaaccgcc
     4741 cggccggtag gggatgcctg gcccggattc gtgaggaagg tatgccgaaa gcaaaattaa
     4801 gccgcggatt tcgcggatgg gcgcagataa aaataccgaa atccgcgtcc cgaaatccgc
     4861 ggttaaaaac tccactctgc agacttaaga gctgaaggcc gagtaacgtg cgattgctaa
     4921 aaactgtcca gttgcgtaat cacctgttag gcgcttctac acgcaccatg taagcgacga
     4981 aaggcaatgt cccgaagcta ccgcagccag aaagaatccg taacggccga gcgacgcatc
     5041 gagcgcactc gcgacggtag cccacgtctt ccgcacatta tcgagcgcgc cccggcacct
     5101 ggcgactcgc acccgctccc gaagcgggtc ttgcatcggt ttttgcagcg agtccctgtc
     5161 gagttcattt acggtctatc ccgcatcgag cttcgcgccc gcgagttatc gcttattggc
     5221 gcaccattcg gttcctactg ggttggcgag cgcgctatcg tcctctactc gctaccgccg
     5281 gtcttccact gccgttcttt gtcctcggac tttcgcggct cccttaagcg cttccacgcc
     5341 accatccaat ccgatgccaa cggagttact gtctcgtggc gggaaccggc ggtcatgtcc
     5401 atgtggtttt atagctttgt ctttactcac gagcttggcc atcacttcgt tgagcaatac
     5461 aaatccaaga acagtcgtat ccattcccgc aacgcagaag agcttgttgc cgatctccac
     5521 gcaaagcgat ttacagatga gcttttcgca tccttccgca agcgacgaag catctaatta
     5581 tgcgctgaac ttggggtcgt taggcgcttc tgaacttgtc cccaaatcgg cagacgttat
     5641 tctccagcca tgatcctcga aaccattccg gacgtgaatc gactgacacc cgcccaaaag
     5701 cttcttctgg taagcgagct ttgggacgat ctggcagc
//
LOCUS       DATDAY010000383         2392 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_1728145, whole genome shotgun sequence.
ACCESSION   DATDAY010000383 DATDAY010000000
VERSION     DATDAY010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2392)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2392)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2392
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1728145"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..467
                     /locus_tag="VIS74_07530"
     CDS             <1..467
                     /locus_tag="VIS74_07530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY5793133.1"
                     /translation="LGRTVLISNYGEYYRLVNYLTRYTDKMIGLPLGIPAMHEIFDEK
                     YYTHLEGGILEGLGRLFKTGVKLYVYPKLEENGRLSTADNFQVAPNLKHLYSHLLENG
                     FIESMRGYHSDYFGIYSPNVLQKIRAGDLSWKTHVPVEVARVIEARGLFMTH"
     gene            complement(642..1145)
                     /locus_tag="VIS74_07535"
     CDS             complement(642..1145)
                     /locus_tag="VIS74_07535"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEY5793134.1"
                     /translation="MKRFASLFCAAAIALSLGACNKNRKSGEYAGVDGDFITGTPLPE
                     RIEGANFLGANVSRGEYAPVYFNYDSFEVSGAEQGKVDALAKAMKDWPNSIIIAGFTD
                     SRGTDEYNRGLGERRAQAVRQSLIAAGVDGARVQTVSFGKEMPADPAETEAAYAKNRR
                     AEFGVVK"
     gene            complement(1184..2338)
                     /locus_tag="VIS74_07540"
     CDS             complement(1184..2338)
                     /locus_tag="VIS74_07540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5793135.1"
                     /translation="MKKPFSLLLVLTSLTLGAFAQAQEPMTITVKKGDTINVAFKGLS
                     GADAEAISKVLLNDLAISGTINVGVPERATYTISGTAGSASLQGSVADSRDSAVLTKT
                     YTGNARSLAHQFADDIVETLSGKPGIANTKIAFASSRSGRKEIYLADYDGANVQQITR
                     DNAISVAPALSADGSKLAYTGYQSGYADIYLVQLGSGARNRIVKFPGTNSGAAFSPDG
                     SRIACTISKDGNPELYTVSASGGGARRLTRTKGVESSPTWSPDGREIIFTSDENGSPQ
                     LNRISSAGGSGTPISTGFGFCTEPSWSPDGRKVAFTVRSGGFSIAVKDLSGGPGQILV
                     SGQDPVWGPNSRHLIYSTGSSLVLLDTQTRKTYPIISGLGQVGEPTWARK"
BASE COUNT          427 a          699 c          794 g          472 t
ORIGIN      
        1 cgctcggacg cacggtgctg atctccaact acggcgaata ttaccgcctc gtgaattacc
       61 tcacgcgcta tacggacaag atgatcggcc tgccgctggg cattccggcc atgcacgaga
      121 tcttcgacga aaaatattac acccacctcg agggcggcat cctggaaggc ctcgggcgcc
      181 tgttcaaaac cggggtgaag ctctacgtgt atcccaagct ggaggagaac ggccggctga
      241 gcaccgccga caatttccag gtcgcgccga atctcaagca tctttactcc catcttctgg
      301 aaaatgggtt catcgaaagc atgcgggggt atcactccga ttatttcggc atttattcgc
      361 ccaatgtgct gcagaaaatc cgcgcgggcg acctcagctg gaagacgcat gtcccggtcg
      421 aggtggcccg ggtgatcgaa gcgcgcgggc ttttcatgac gcactgaatc cgaaaacggc
      481 ttcggcttca ggcggctggc cgcgcctgta gccgggatcg ttgatcccgg ctttccctgc
      541 gttcgttccc ctccggccgg gaccagcggt cccggctgcc ggcgataaaa aaagccgctc
      601 cgaagagcgg cttttttgat aaagacggtc gaaggcggcg cttatttgac cacgccaaat
      661 tccgcgcggc ggttcttggc gtaggccgct tcggtttccg ccgggtcggc cggcatttcc
      721 ttgccgaagc tgacggtctg gacgcgcgcg ccgtccacgc cggccgcgat gagcgactgg
      781 cggaccgcct gggcgcggcg ttcgccgagg ccgcggttgt attcatccgt tccgcgcgaa
      841 tcggtgaagc cggcgatgat gatgctgttc ggccagtcct tcatggcctt ggccagcgcg
      901 tccaccttgc cctgctcggc gccgctgact tcaaagctgt cgtagttgaa atagaccggc
      961 gcatattccc cgcgcgagac gttggcgccg aggaagttcg cgccttcgat gcgctccggc
     1021 agcggcgttc cggtaatgaa gtccccatcc acgccggcgt attcgccgga ttttcggttt
     1081 ttattgcagg cgccgaggct gagcgcaatc gcggcggcgc agaagagaga ggcaaagcgt
     1141 ttcatgggat gtgtgaatga cggatttccc ggcaaatttc aaatcatttg cgagcccacg
     1201 tgggttcgcc gacctggccg aggccggaga tgatcgggta ggttttccgc gtttgggtgt
     1261 ccagcaatac caggctggat ccggtggaat aaatcaggtg ccgcgaattc gggccccaga
     1321 ccggatcctg gccggagacg aggatctgcc ccggccctcc gctcagatcc ttgacggcga
     1381 tggagaatcc gcccgaacgg acggtgaagg caaccttccg gccatccggc gaccagctgg
     1441 gctcggtgca gaatccgaaa ccggtggaaa tcggcgtccc cgaaccgccg gcggagctga
     1501 tgcggttaag ctgcgggctg ccgttctcgt cggaagtaaa aatgatttcc cggccgtccg
     1561 gcgaccaggt cggcgaggat tccacgccct tggtccgggt caggcggcgg gccccgccgc
     1621 cactggcgga gaccgtgtaa agctcgggat tcccgtcttt gctgatggtg caggcgatcc
     1681 ggctgccgtc gggcgagaag gccgcgccgg agttggtgcc ggggaatttg acgatgcgat
     1741 tgcgcgcccc gctgccgagc tggacgaggt aaatgtccgc gtaaccgctc tggtaaccgg
     1801 tgtaggcgag cttggagccg tccgcgctca gggccggggc gacgctgatg gcgttatccc
     1861 gcgtgatctg ctggacgttg gcgccgtcat agtccgccag gtagatttcc ttgcggccgg
     1921 agcgcgagga ggcgaaggcg attttcgtat tcgcgattcc cggcttgccg ctgagggttt
     1981 ccacgatgtc gtcggcgaat tggtgcgcga gcgagcgggc gttgccggtg taggttttgg
     2041 tcagcacggc ggaatcgcgc gagtccgcca ccgagccttg caggctggcg gaacccgcgg
     2101 tgccggagat cgtgtaggtg gcgcgctcgg gaacgccgac gttgatcgtt ccggagatcg
     2161 cgagatcatt caacagcacc ttcgagatgg cctcggcgtc cgcgccgcta aggcccttga
     2221 aggcgacatt gatcgtatcc ccctttttga cggtgatcgt catgggttcc tgggcttggg
     2281 cgaaggcgcc caaagtcagc gaggtcagga cgaggagaag ggaaaagggc tttttcatga
     2341 atggaaaaga cggataaggg atgccagagt ttccgggtga attcaatgcg ca
//
LOCUS       DATDAY010000384         2662 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_1383121, whole genome shotgun sequence.
ACCESSION   DATDAY010000384 DATDAY010000000
VERSION     DATDAY010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2662)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2662)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2662
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1383121"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(171..932)
                     /locus_tag="VIS74_07545"
     CDS             complement(171..932)
                     /locus_tag="VIS74_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006983552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5793136.1"
                     /translation="MKWLALLLVSLAAAGAQSIQLSDAQALEIGRRIWKNECAGTVDG
                     LTSWNGGEAFASLGIGHFIWYPTARKGIYEESFPKLVVYFQQRGVKLPSWLTPDALCP
                     WPNRAAFQKDFQSPQMRALRELLARTVPLQAEFAALRLEAALPKMLQRAPAGEREKIR
                     KNFYRVAAEPLGLYALMDYVNFKGEGTSPEERYRGQGWGLLQVLEGMGNGPALKEFAA
                     SADRALTRRVGNAPPERKEARWLPGWRNRLKTYVD"
     gene            complement(929..1471)
                     /locus_tag="VIS74_07550"
     CDS             complement(929..1471)
                     /locus_tag="VIS74_07550"
                     /inference="COORDINATES: protein motif:HMM:NF020398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1802 family protein"
                     /protein_id="HEY5793137.1"
                     /translation="MKPAFKEWAVVCEALGTGRQSLIIRKGGIAEGREGFAFKHEEFF
                     LFPTWFHEQIAKTRLPPDTPLPPEPTDEIDIAYIANLDWSRLVTDREKVNQLREYHIW
                     QDSVVDERFEYDEPCGVHVALVRVYRLDPPVRLKLEKKYGGCRSWVELPELEGSALVS
                     VISDEAHAERRRALERILDL"
     gene            complement(1468..1938)
                     /locus_tag="VIS74_07555"
     CDS             complement(1468..1938)
                     /locus_tag="VIS74_07555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5069 domain-containing protein"
                     /protein_id="HEY5793138.1"
                     /translation="MTPRKFLGKLSLMKYPRSPHEKVGGIVYFGRMVDKIRLMAANEL
                     HPDLHANLGKGFDERCVHFLNVNYGALAEEVRRGATDEAALAWCFEKGTRPTEEQIEI
                     WNDFMRKRAWNDEVAATLIRRKKESGLENRDDIQTMFDYIDADEGRPLQPKITQ"
     gene            1949..2653
                     /locus_tag="VIS74_07560"
     CDS             1949..2653
                     /locus_tag="VIS74_07560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEY5793139.1"
                     /translation="MLHRFELHCHSWYSADAADSPEDLIARARAQGLSGLAITDHNTC
                     DAAAYLESQGLLRPDGLPVEGFLVIPAVEVSTADGHLICLGVTLPNRVGVPAVEVAKE
                     IRARGGVPIPAHPYDRWRAGIREEVLDQLPIRVIEVFNAAVTSREYNVAAHQYAAARG
                     LVGMAGSDAHHASAIGTAVTALELESLSVANVLAQIEIGAALEERYLNRWEGVKKHFG
                     NWFRVFNRRPKPEPSN"
BASE COUNT          464 a          809 c          849 g          540 t
ORIGIN      
        1 ggcgcggatc ttgaagggat tttcgccctt gatttcgagc aggcgggcga tgttttcaaa
       61 aatttcggcg atttcgtctt tggtcatggt gcggaggaag gaaaggcgcg ttccggttta
      121 acccgggaac ggcttttgaa ccccagtcaa ttcaacaacg gttttcgggt ttaatcgacg
      181 taagttttca ggcgattgcg ccatcccggc agccagcggg cttccttgcg ctccggcggg
      241 gcgtttccca cgcgacgggt cagcgcccga tccgcggagg cggcgaattc cttgagcgcg
      301 gggccattgc ccatgccttc gagcacctga agcaggcccc agccctggcc gcggtaacgc
      361 tcctccggcg aggtgccttc gcccttgaag ttgacgtaat ccatcaatgc atacaacccg
      421 agcggttccg ccgccacgcg atagaagttc ttccggattt tttcgcgctc acccgcgggc
      481 gcgcgttgca gcatcttggg cagcgccgct tcgagccgca gcgcggcgaa ttccgcctgc
      541 agcggcacgg tgcgcgccag cagctcgcgg agcgcccgca tttgggggct ttggaagtcc
      601 ttttggaacg ccgcgcggtt cggccagggg cagagcgcgt cgggagtcag ccaggagggg
      661 agtttgacgc cccgctgttg gaaatacacg acgagcttcg gaaaactttc ctcgtagatg
      721 cctttccgcg ccgtcggata ccagatgaaa tgcccgatgc cgagggaggc gaacgcttcg
      781 cccccgttcc acgaggtcag cccgtccacc gttcccgcgc attcattttt ccaaatgcgc
      841 cggccgatct cgagcgcttg cgcgtcgctg agttgaatgg attgagcgcc ggccgcggcg
      901 agcgaaacga ggagaagggc cagccacttc ataaatcgag aatgcgttcc agcgcccggc
      961 ggcgttcggc gtgggcttca tcgctgatga cggaaaccag cgccgagccc tccagctcgg
     1021 gcagttcgac ccacgagcgg cagccgccgt attttttttc gagcttgagg cggacgggcg
     1081 ggtccagccg atagacccgg accagggcga catgcacgcc gcagggttcg tcgtattcaa
     1141 accgctcgtc caccacggag tcctgccaga tgtggtattc gcgaagctgg ttcacctttt
     1201 cgcggtcggt caccagccgg ctccaatcca gattggcgat gtaggcgatg tcgatctcat
     1261 ccgtgggttc cggcggcaac ggcgtgtccg gcggcagccg cgttttcgcg atctgctcat
     1321 ggaaccacgt ggggaacaga aaaaattcct cgtgcttgaa cgcgaagccc tcccgcccct
     1381 ccgcgatgcc gcccttgcgg atgatgaggc tctgtcggcc ggtccccagc gcttcgcaaa
     1441 cgacggccca ttccttgaag gcgggcttca ttgcgtgatc ttcggctgca acggccggcc
     1501 ctcgtcggcg tcgatgtaat cgaacatggt ttgaatgtcg tcccggtttt ccaagccact
     1561 ttcttttttc cggcgaatga gcgtggcggc cacctcgtcg ttccaggcgc gtttccgcat
     1621 gaagtcgttc cagatttcga tttgctcctc ggtggggcgg gtgccctttt cgaaacacca
     1681 ggcgagggcg gcttcatccg tggcgccgcg ccggacttcc tcggcgagcg cgccgtaatt
     1741 gacgttcagg aaatgcacgc aacgttcgtc gaagcccttg cccagattgg cgtggagatc
     1801 gggatggagc tcattggcgg ccatgaggcg gattttgtcc accatgcgcc caaaatagac
     1861 aatgccaccg actttttcgt gggggctgcg cgggtatttc atgagcgaga gcttgcctaa
     1921 aaatttccga ggcgtcacgt ttttgtgaat gctccatcgt ttcgaactcc attgccacag
     1981 ctggtattcc gccgatgccg ccgattcgcc cgaggacttg atcgcccgcg cccgcgcgca
     2041 ggggctgagc ggcctggcca ttaccgatca caatacctgc gatgcggcgg cgtatctgga
     2101 atcccagggg ctgctccgcc ccgatggcct gccggtggag ggatttctgg tcattcccgc
     2161 cgtggaggtc tccacggcgg acggacatct gatttgtctg ggcgtgactt tgccgaatcg
     2221 cgtgggcgtt cccgcggtgg aggtggcgaa ggaaatccgc gcccggggcg gggtgccgat
     2281 ccccgcgcac ccatatgacc gctggcgggc gggtattcgc gaggaggttt tggaccaact
     2341 gccgatcagg gtcatcgaag tcttcaatgc cgcggtgacc tcgcgggaat acaacgtggc
     2401 cgctcatcaa tacgccgcgg cccggggact ggtcggaatg gccgggagcg acgcgcacca
     2461 cgcgagcgcc atcggaacgg cggtcacggc attggaattg gaatcgctca gcgtggccaa
     2521 tgtgctggcg cagatcgaaa tcggggccgc gctcgaagag cgttacctca accgctggga
     2581 gggcgtgaaa aagcacttcg ggaattggtt ccgggtattc aaccgccgcc cgaagccgga
     2641 accttcaaac taaacgggat ta
//
LOCUS       DATDAY010000385         2501 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_244240, whole genome shotgun sequence.
ACCESSION   DATDAY010000385 DATDAY010000000
VERSION     DATDAY010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2501)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2501)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2501
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_244240"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2047)
                     /locus_tag="VIS74_07565"
     CDS             complement(<1..2047)
                     /locus_tag="VIS74_07565"
                     /inference="COORDINATES: protein motif:HMM:NF036578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5793140.1"
                     /translation="MSKVRPGLVVKNHDLSLPDWGPYGKRHAGASHIADRENGLRFDL
                     SVFPALYRRKADVPSAGWESGYHPWASAPRFEYFSFRHEILWKDQVYADISYSRLAGH
                     SGLLVRCELVNATPAATPAALHFMASLAYPSPWQNSREPLRKLVPRLPEGAVLIHPLD
                     YSRLDFAGPEPMDALGPDGSMRGETRGQEFLDGSALGGRFGRDEGDAVTYCIDLDAPV
                     SGAHLVIRCRSTSQRGQSEWNLRVASPDSGEPREIPLQVKGADPENHVIPLGKLNAGR
                     LSLTFASRRADSALELVFLALAPGAAFSGDVPVRVAGPQWTPERLPGAPGRSLILKYP
                     DAPSCYGLAWEAEPFEVRQILHSELDSFLRAKTHDHVNETLLGDGQGHFTNVFLRPIT
                     LPPHSRRVIWGLVCEGSPEGVGEILAAFAAGGIDKETIHAEAKKKKFAFAPEAAGRAQ
                     AFGQNLMAAVTLTNAVFPIYMQRQQVRHRTPGRLWDSLYTWDSGFIGLGLLEIDPAQA
                     EENLAQYLTEPGNPHAAYVHHGSTVPMQIHLYHELWNRTQSRELLERHYASIRQYYRF
                     FAGHAAGSTLRNLKSGLLRSWDYFYNSGGWDDYPPQKHVHAHRLTDRVTTASVTAHAI
                     RCARSLALLAGLLEKPGDAAEYRRDIETFSAALQTLAWDPAAGYFSYVTHDAQGAAEG
                     "
BASE COUNT          468 a          761 c          830 g          442 t
ORIGIN      
        1 ggccctccgc cgcgccctgc gcatcgtgcg tgacatacga aaaataaccc gccgcgggat
       61 cccacgcgag agtttgcagg gcggcggaaa acgtttcgat gtcgcgccgg tattcggcgg
      121 catccccggg cttttcgaga agcccggcaa ggagagccag gctccgggcg cagcggatgg
      181 cgtgcgcggt gacgctggcg gtggtcaccc ggtccgtgag ccgatgggcg tgaacgtgct
      241 tttgcggcgg atagtcgtcc catccgcccg agttgtagaa atagtcccag cttcgcagca
      301 ggccggactt cagattccgc aaggtggacc ccgccgcgtg gcccgcgaaa aatcgataat
      361 attgccggat ggaggcgtag tgccgttcca gcagttcgcg ggactgcgtg cggttccaga
      421 gttcgtgata aaggtgaatc tgcatgggca ccgtgctgcc gtgatgcaca taggcggcgt
      481 gcgggttgcc gggttcggtg aggtattgcg cgaggttttc ctccgcctgg gccggatcga
      541 tttccagcag cccgagcccg atgaagccgg aatcccaggt gtagagcgaa tcccacaacc
      601 gccccggcgt gcgatgccgg acctgctggc gttgcatgta aatggggaat acggcgttgg
      661 tcagcgtgac cgccgccatg agattctggc caaacgcctg agcccggccg gccgcttccg
      721 gcgcgaaggc gaacttcttc tttttcgctt cggcgtggat ggtttccttg tcgatgccgc
      781 cggcggcaaa tgccgcgagg atttcgccca ctccctccgg actcccttcg caaaccaagc
      841 cccaaatcac gcgccgcgag tgcggcggca gcgtgatggg gcgcaggaag acgttcgtga
      901 agtgtccctg gccgtcgccc agcagggttt cattcacgtg atcatgggtt ttggcgcgca
      961 ggaaactgtc gagttcgctg tgaagaatct gccgcacttc aaatggctcc gcctcccagg
     1021 ccaggccgta gcaggacggc gcgtcgggat atttcaggat gagcgaacgg cccggcgcgc
     1081 cgggcagccg ctccggggtc cattgcgggc cggcgacgcg cacgggcaca tcgcccgaaa
     1141 aagcggcgcc gggcgccaaa gccaggaaga caagctccag cgcggagtcc gcgcggcggg
     1201 aggcaaacgt cagcgaaagt cgcccggcat tcaatttgcc caggggaatg acgtggttct
     1261 cggggtccgc gcctttcacc tggaggggga tttcccgcgg ctccccgctg tccggagaag
     1321 cgacgcgcag gttccattcg ctttgaccgc gttgcgaggt cgaacggcag cggatgacca
     1381 ggtgtgcgcc ggagacgggc gcatccaggt caatgcaata cgtgaccgcg tcgccctcat
     1441 cccggccgaa acgcccgccc agcgcgctgc catccaaaaa ctcctgcccg cgcgtctcgc
     1501 cgcgcatcga tccgtccggc ccaagcgcgt ccatcggttc cggcccggca aagtcgaggc
     1561 gggagtagtc aagcggatgg atcaaaacag cgccctcggg caaccgggga acgagttttc
     1621 gcaggggttc ccgcgagttt tgccacggtg agggataagc cagggaggcc atgaaatgca
     1681 gcgcggccgg agtggcggcc ggggtggcat tcacgagctc gcacctcacc aggagtccgg
     1741 aatgcccggc aagccgggaa tacgagatgt cggcatacac ctggtctttc cagagaatct
     1801 cgtgacggaa gctgaaatat tcgaaccgcg gggcgctggc ccagggatga taccccgact
     1861 cccatccggc gcttggcacg tccgccttcc ggcgatacag cgcggggaag acactcagat
     1921 caaagcggag gccgttttcc cggtcggcga tgtgggacgc gcccgcgtgg cgtttgccat
     1981 acggccccca atccgggagc gacaaatcat gattctttac aaccaaaccg ggccggactt
     2041 ttgacatgag gaagaaattt ttgagggatg ggtcacggag gtcattaccg aaattcatcc
     2101 gggcatttca ccctccaaag gaagcgaatc atgcaggttg tttccctttt gaaaacattt
     2161 ttatcggttt gaaggaaagg ccgaaagatc aggaaaagcg cggccccccc agcgcacggc
     2221 ttgccggcgg cgcttgtctg cctcggcgga ccgggaaaat aggcgggcac gcacccgcgg
     2281 gcggcgggat gcttggcgat tcggacgctt aaagggtgta ccgaaagccg gcagcacgga
     2341 gttctttaac cgcgcccgcc catccgcggt ttaattccgg ctttcggcac ccccctttta
     2401 ggctgcgcgc tcgaggcttc gcgccagggc gtgcagggtt tgtgaatcct cagcggcaag
     2461 acttccgaga gcgtgtcgga acatctggtc gtggcggtcg a
//
LOCUS       DATDAY010000386         3525 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_852448, whole genome shotgun sequence.
ACCESSION   DATDAY010000386 DATDAY010000000
VERSION     DATDAY010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 3525)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3525)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3525
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_852448"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1041)
                     /locus_tag="VIS74_07570"
     CDS             complement(<1..1041)
                     /locus_tag="VIS74_07570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HEY5793141.1"
                     /translation="MSKEKADLRAKLNDVPHKPGVYLMRDRFSRVIYVGKARDLRKRL
                     AHYFTPSRQARAELKTRALIDSIWDFECHLVRSDAEAVLYEGRLIKEFRPKYNVSFRD
                     DKRFLLVRVNLNDPFPRFQLTRIRKEDGARYFGPFAHSGALRSTLNAMRKRFGLRSCR
                     PFIPGETDYKHCLDHIIKQCSAPCVAKITREAYHAKVKEACEFLEGRSSEMLSQIEEE
                     MKKAAAALNFEKATELRNMLDDIKSTTRQERQKKFVREIPSTVIPEHDLEELRDALQL
                     QSVPAVIECFDISNISTTHKVASMVQFRNGRPDRSNYRRYRIKTVEGQDDFASMAEVV
                     GRRYARVLRESGN"
     gene            1156..1974
                     /locus_tag="VIS74_07575"
     CDS             1156..1974
                     /locus_tag="VIS74_07575"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HEY5793142.1"
                     /translation="MKKYLLAFLFASASFALAEPVDAAAPAVSAQPLSLWQVLQSGGV
                     VMVPLGVLSIIAVALILIYLFTLTRGRMVSARYVQTAEALIRKKDWLGLIAISSRHNE
                     ATADVVLRMTDFVTKNPDASLDEAREIAEAEGTRLASRFNQQVVYLADIGTIAPMLGL
                     LGTVLGMVQSFNAVAADAASTTRPLLLAAGVSEALIATAAGLCIGIPAMAAYAYFRGR
                     LQALLADFEAATTHLLALFAANYVPGEISRPTESLESTRASGLPPKPQRRTALI"
     gene            1971..>3525
                     /locus_tag="VIS74_07580"
     CDS             1971..>3525
                     /locus_tag="VIS74_07580"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY5793143.1"
                     /translation="MRWLAPWCVLGILSPAFAQEIRRATPVESPSPNPVDRIQNPSWM
                     QMLPPRTATPAPAPAEPMVRPALPVEPVPPRVNQPAGQEAPPIRPALPAGTPYRPAAR
                     AVPVEAAPTPAPSPTPIPTPTPDPGNAIRITAGETPADPAQAALQRADSFYSRKLYDL
                     AIPDYEAYLRLSRPKSKGRDAALFRLAECQRFLERGDAAQAIYERLLDEFSQGEFASS
                     GAYRLGEIFFGKGVYALAAPQFEKAGQTTPHEAVRLSADFYFARSLEEMREDARALAA
                     YEKVRQSPVKENPYRDYAQTAAARLAAKVGDKKRAAELFTAISQTGGSEELKAEAAVK
                     AAGLWLELGDGKPARQLLDQVIKNGKSAKWAGVAQTTLADLDYQAGDYARLAQLADKL
                     DTMPAEVQPRILLVAANSRRQLSQHTKALELYDRLMREFPNSPEVQSARFQRLVSLYE
                     TGNPGLAEELNQFLFATYDPKERNQATMLKAEMLFKAGRYAEAASAYQPLPDADLPDN
                     LKAESLYKLA"
BASE COUNT          599 a         1104 c         1155 g          667 t
ORIGIN      
        1 atttccactt tcgcgcaaaa cacgtgcata acgccgcccc accacttccg ccatgctggc
       61 aaaatcgtcc tgcccttcca ccgttttgat gcggtagcgg cggtagttgg agcggtcggg
      121 ccgtccgttg cggaactgca ccatggaagc cactttgtgc gtggtggaaa tgttcgagat
      181 gtcaaaacac tcgatgaccg caggcaccga ctgcagctgc aatgcgtcac gaagctcctc
      241 cagatcgtgc tcgggaatca ccgtgctggg gatttcgcgg acaaattttt tctgcctctc
      301 ctggcgggtg gtggatttga tgtcgtccag catgttgcga agttcggtgg ccttttcaaa
      361 attcagggcc gccgcggctt tcttcatctc ctcctcgatc tggctcaaca tttcggaaga
      421 ccggccttct agaaattcgc aggcttcttt cactttggcg tgataggctt cacgggtgat
      481 tttggccacg cagggggcgg aacattgttt gatgatgtga tccaggcagt gcttgtaatc
      541 cgtctcgccg ggaatgaacg ggcggcagga gcgcaggccg aagcgcttgc gcatggcgtt
      601 gagggtggag cgcagcgcgc cgctgtgggc gaaggggccg aaataacgcg cgccgtcctc
      661 cttgcggatg cgggtgagct gaaagcgtgg gaaggggtcg ttgaggttga cgcggacgag
      721 caggaaacgc ttgtcgtcgc ggaagctgac gttgtatttc gggcggaatt ccttgatgag
      781 ccgcccttcg taaagcaccg cctcggcgtc gctgcggacg aggtggcatt cgaagtccca
      841 gatgctgtca atgagcgcgc gggtcttgag ctccgcccgg gcctgccgcg agggcgtgaa
      901 ataatgcgcc aggcgcttgc ggaggtcgcg cgccttgccc acgtagatca cgcggctgaa
      961 gcgatcccgc atcagataca cgccgggttt gtgcggcaca tcgttcagct tcgcccggag
     1021 gtcggctttc tcttttgaca tcggtccaaa ataagctaag ggatttcaca aggttggttc
     1081 tgccggaagc gggcagctga aagaagcgga acccggcttt tctcgggttt ccggcagagt
     1141 gaaccatatg acatcatgaa aaaatacctc ttggccttcc tttttgccag tgcttccttc
     1201 gcgctggccg agccggttga cgcggcggct cccgcggttt ccgcccaacc gctcagtctc
     1261 tggcaggtgc ttcagtcggg cggcgtggtc atggttccgc tgggggtgct gtccatcatc
     1321 gcggtggccc tcatcctgat ttatcttttc accctgacgc gggggcgaat ggtttccgcg
     1381 cgctacgtgc agaccgccga ggcgctgatc cgcaaaaagg actggctggg actcatcgcg
     1441 atttcctcgc gccataatga agccaccgcc gatgtcgtcc tgcggatgac ggattttgtg
     1501 accaaaaatc cggatgcgag cctggatgag gcgcgcgaga tcgccgaagc cgagggcacg
     1561 cggctggcga gccggttcaa ccagcaggtc gtttatctcg ccgacatcgg gacgatcgct
     1621 ccgatgctcg gattgctcgg cacggttttg ggcatggtgc aatccttcaa cgccgtcgcc
     1681 gccgatgcgg cctcgaccac gcggccattg ctgctcgcgg ccggggtttc cgaagcgttg
     1741 atcgcgaccg ccgccgggct ttgcatcggc attccggcca tggcggcgta tgcgtatttc
     1801 cgcgggcggc tgcaggcgct cctcgcggat tttgaagcgg cgacgaccca tctgctggcg
     1861 cttttcgccg ccaattacgt tcccggggag atcagccgtc cgaccgagtc gctggaatcc
     1921 acccgcgcct ccggcctgcc gcccaagccc cagcgccgga ccgccctgat atgaggtggc
     1981 tggcgccgtg gtgtgtcctc ggcattttaa gcccggcctt cgcgcaggaa atccgccgcg
     2041 ccacgccggt ggaatcgcct tcccccaatc cggtggaccg gattcagaat ccctcgtgga
     2101 tgcagatgct gccgccccgg acggccaccc cggcgcccgc gccggccgag ccgatggtcc
     2161 gtcccgcgct tccggtcgag cccgttccgc caagggtgaa ccagcccgcc ggccaggaag
     2221 cgccgccgat tcgcccggcc ctgccggccg gcacgccata ccggccggcg gctcgtgcgg
     2281 tgcccgtgga ggccgcgccc accccggcac cttccccgac gccgattccc acgcccaccc
     2341 cggaccccgg gaacgccatt cgcatcaccg ccggcgaaac gcccgcggat ccggcccagg
     2401 ccgcgctgca gcgggcggac agtttttatt cgcggaagtt gtatgatctg gcgattccgg
     2461 attacgaggc atacctgagg ctttcccggc ccaagtcgaa ggggcgggat gcggccttgt
     2521 tccggctggc ggagtgccag cgttttctgg agcgcggcga cgcggcgcag gcgatctacg
     2581 agcggttgct ggacgagttt tcccaggggg agttcgcgag ttcgggggcc taccggctgg
     2641 gcgagatttt tttcggcaag ggggtttacg ccctggcggc gccgcagttc gagaaggcgg
     2701 gccagaccac gccgcacgag gccgtccgcc tttccgcgga tttttatttc gcccgctcgc
     2761 tggaggagat gcgggaggac gcccgggcgc tggcggctta cgagaaggtg cggcaaagcc
     2821 cggtgaagga gaatccctac cgcgactacg cccagaccgc cgccgcgcgg ctcgcggcca
     2881 aggtgggcga caaaaaacgc gcggccgagc tgttcacggc catttcgcaa accggcggca
     2941 gcgaggagct gaaagcggag gccgcggtca aggcggcggg attgtggctg gagctgggcg
     3001 acgggaaacc ggcgcggcag ctgttggacc aggtcatcaa gaatgggaaa tcggcgaaat
     3061 gggcgggagt cgcgcaaacc acgctggccg atctggacta ccaggccggc gactacgccc
     3121 ggctggcgca gctcgcggac aagctcgaca cgatgcccgc ggaggtccag ccccggattc
     3181 tgctcgtggc ggcgaattcc cgccgccaat taagccagca caccaaggcg cttgagctct
     3241 acgaccgcct catgcgggaa tttccgaatt cccccgaagt ccagagcgcg cgcttccagc
     3301 ggctggtgag cctttacgag acgggcaatc ccggcctggc cgaggaattg aaccaatttc
     3361 tcttcgcgac ttacgatccc aaggagcgca atcaggcgac gatgctcaag gccgagatgc
     3421 ttttcaaggc ggggcgttac gccgaggcgg cctcggcgta tcagccgctg ccggacgccg
     3481 atttgccgga caatctgaag gcggagtcgc tttacaaact cgcct
//
LOCUS       DATDAY010000387         3796 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_677673, whole genome shotgun sequence.
ACCESSION   DATDAY010000387 DATDAY010000000
VERSION     DATDAY010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 3796)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3796)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3796
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_677673"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..979
                     /locus_tag="VIS74_07585"
     CDS             <1..979
                     /locus_tag="VIS74_07585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5793144.1"
                     /translation="AKTPEAVYRIQDELELFRDEELSNGARATRPLNDRLNRAINFIN
                     NWQQVITAEANGEFAEAVQSIGNLRRNAYASALMGPAALNAKYQSLLAQALNPPESAK
                     ESSPIRAAIAAALQKVKTPADAAAALEVLNQVASFSSGDDNRLANHVQNELRGLVEMN
                     HEFAAGAYARVIGRSSDSASGPYARQLESLKNELRLKAIAAANDLPDLGTPPAAEGLP
                     TFIRRLAVEAFEKKDWERLYTLLSVYSTLTGGGCARTQDMKQGVSAYLAGQQLEQAGQ
                     FGDAVSQYTLCLTYLGKLIPRADAAKALDRLRLSHPEAFAPAGNLRGAN"
     gene            1078..2466
                     /gene="lpdA"
                     /locus_tag="VIS74_07590"
     CDS             1078..2466
                     /gene="lpdA"
                     /locus_tag="VIS74_07590"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEY5793145.1"
                     /translation="MAYDLIVIGGGPAGYVGAIRAAQLGKKVACVEMDRGGGTCNNWG
                     CIPTKALLKNAEMYHDFKHRSAEFGFKLEGLEYDWTKIIKRSRDVSDKGAAGVDYLFK
                     KNKIDSIKGVATLEKAGEVKVKLPDGKEQILTGSKILVATGCVSRPMPGFPYNGKTVI
                     SSRDALVLPEQPKSVIVIGAGAIGIEFAYFWNSFGTKVTVVEMMPHILPVEDSEVALA
                     LDKSLAKQGLTILAGHKTVKTEADDKGVKITVADEKGAEKTLEADMALVAIGIQPLLP
                     EGALKLGLTDRGYIQTNENYETTVKGIYAAGDIIGPPWLAHVASYEATQAVEGMFIPG
                     YKPRKPTLFPGCTYCHPQVASIGLTERAAKEKGIPFKVGKFAFMASGKARAISDTEGF
                     VKLLIGEKHGEILGAHIIGADATEMLAELGLAITLEATYEELVNTIHAHPTLSEAVHE
                     AAHASQGHAIHS"
     gene            2550..3125
                     /locus_tag="VIS74_07595"
     CDS             2550..3125
                     /locus_tag="VIS74_07595"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY5793146.1"
                     /translation="MGHLLFLQEFFKAASTTGAVLPSSPRLAELLVEKANVRNSREIL
                     ELGPGEGVVSREILKAKGGESRFLAVEKNPRFVELLKSRNPEVPVIHGCGTRLAEHSR
                     DHRMHSVDCVVSCLPWASFPPSLQSDILKQVLATLKPGGTFATFAYFGPHWLPAGQRF
                     RKQLQSAFSRIHTSRIELVNFPPAFVYYSRR"
     gene            3154..3585
                     /locus_tag="VIS74_07600"
     CDS             3154..3585
                     /locus_tag="VIS74_07600"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEY5793147.1"
                     /translation="MPSRIQRQSTTPTTAAARIKGPKEVSVMAWIENQFGEVLLLKQS
                     NGRRLWTLPGGKVRTREALATALRREVLEETGLQLKSLEFAHLFDRPRKSTITFVYRV
                     TLRGKDTVVPKANEIETAQYWATLPRSATPSLAFFWKQRRG"
BASE COUNT          774 a         1201 c         1162 g          659 t
ORIGIN      
        1 ggcgaaaacg ccggaggcgg tttatcgcat tcaggatgaa ttggaactct tccgggacga
       61 ggagctcagc aatggcgccc gcgccacgcg gccgctcaac gaccggctca accgcgccat
      121 taatttcatc aacaactggc agcaggtcat taccgcggaa gccaatggcg aattcgcgga
      181 agccgtgcag tcgatcggca atcttcgccg gaatgcctac gcttcggcgc tgatgggccc
      241 cgccgcgctc aacgccaaat atcaatccct gctggctcaa gcgctcaacc cgccggaaag
      301 cgcgaaggaa tcctcgccga tccgcgcggc catcgccgcg gcattgcaaa aggtcaaaac
      361 cccggccgac gcggcggccg ccctggaggt cctgaaccaa gttgccagct tcagctccgg
      421 cgacgacaac cgcctggcca accacgtgca aaacgagctc cgggggctgg tcgagatgaa
      481 ccatgagttc gccgccggcg cgtatgcccg ggtcatcggg cgttccagcg acagcgcttc
      541 cggcccctac gcgcggcagc tcgagtcgct caaaaacgag ctccggctca aagccatcgc
      601 cgccgccaac gatctgccgg atttgggaac gcccccggcc gccgagggtt tgcccacgtt
      661 cattcgccgg ctggcggtgg aggcgtttga gaagaaggat tgggagcgcc tctacacgct
      721 gctctccgtg tattccaccc tcacgggcgg cggctgcgcg cgaacccagg acatgaaaca
      781 gggcgtcagc gcctatctgg ccggccagca attggagcag gcggggcaat tcggcgacgc
      841 cgtgtcccaa tacacgctct gcctgaccta cctcggaaaa ctcattcccc gggccgacgc
      901 cgcgaaggcg ctcgaccggc tgcggctatc ccatcccgaa gcttttgcgc ccgccggaaa
      961 tctccgcggc gcgaattgag ccgccgcgcg gcgggtgggg aaggggccca tagattcatt
     1021 tgcgctgggc gcggaaatct gttcagttga tttcattcaa cccgttctga attatttatg
     1081 gcttacgatt tgattgtgat aggcggcggt cccgccggtt atgtgggggc aatccgggcg
     1141 gcgcagctcg gcaagaaggt tgcctgcgtg gagatggatc gcggcggcgg cacgtgcaac
     1201 aactggggct gcatcccgac caaggcgctg ctcaagaacg ccgagatgta tcacgacttc
     1261 aaacaccgct ccgcggagtt cggcttcaag ctcgaggggc tggaatacga ctggaccaag
     1321 atcatcaaac gctcgcgcga cgtttccgac aaaggcgcgg cgggagtgga ttacctcttc
     1381 aagaagaaca agatcgattc catcaagggc gtcgccacgc tcgaaaaggc gggcgaggtg
     1441 aaggtcaaac tgccggacgg caaggagcaa atcctcaccg gctcgaagat cctcgtcgcc
     1501 acgggttgcg tgtcccgccc gatgcccgga tttccctaca atggcaagac ggtcatcagc
     1561 tcgcgcgacg ccctggtgct gcccgagcag ccgaagtcgg tgatcgtcat cggcgcgggc
     1621 gcgatcggca tcgagttcgc gtatttctgg aattccttcg gcaccaaggt gaccgtcgtc
     1681 gaaatgatgc cgcacatcct gccggtcgag gacagcgaag tggcgctggc gctggacaag
     1741 tcgctggcca agcagggcct caccattctc gccggccaca agacggtgaa aaccgaggcg
     1801 gacgacaagg gggtgaagat cacggtggcc gatgaaaagg gcgcggaaaa gacgctcgaa
     1861 gccgacatgg cgctcgtggc catcggcatc caaccgctgc tgcctgaggg cgcactcaag
     1921 ctgggcctga ccgaccgggg ttacatccaa accaacgaga attacgaaac gacggtcaag
     1981 ggcatctacg cggccggcga cattattggg cctccctggc tcgcgcacgt ggccagctac
     2041 gaggcgacgc aggcggtcga gggcatgttc attcccggct acaagccgcg gaagccgacc
     2101 ctgttccccg gctgcacgta ttgccatccg caggtggcca gcatcggcct caccgaaagg
     2161 gcggcgaagg aaaagggcat tcccttcaag gtcggcaaat tcgccttcat ggccagcggc
     2221 aaggcccggg cgatcagcga taccgaaggt ttcgtgaagc tgctcatcgg cgaaaagcac
     2281 ggcgaaatcc tgggcgcgca catcatcggc gcggacgcca ccgagatgct cgccgagttg
     2341 ggcctggcaa tcacgctcga agccacctac gaggagctgg tgaacaccat tcacgcccac
     2401 cccacgctca gcgaagcggt tcacgaagcc gcgcacgcct cgcaggggca cgcgattcat
     2461 tcgtgacggg caacgggtga gaagtgccgg tggactcacg cgtccggcca gtcaccctcc
     2521 accagtcgca agtcacccgc caacccacca tgggccatct cctttttctt caggaattct
     2581 tcaaagccgc ctccaccacg ggggcggtcc tgccctcctc gccccggctg gcggagctgc
     2641 tggtcgagaa ggcgaatgtg cggaattccc gggagatcct ggaacttggc ccgggtgagg
     2701 gcgtcgtgtc gcgggaaatc ctgaaagcca agggcggaga aagccggttt ttggccgtgg
     2761 aaaaaaatcc gcggtttgtg gaattgctca aaagccggaa tcccgaggtg cctgtgattc
     2821 acggctgcgg cacccgcctg gccgagcact cgcgggatca ccggatgcac tccgtggatt
     2881 gcgtcgtttc ctgcctgccc tgggcctcgt ttccgccctc cctgcaatcg gatatcctaa
     2941 agcaggtgct cgccaccctg aagccggggg gcacgtttgc caccttcgcc tacttcgggc
     3001 cgcattggct gcccgccggc cagcgtttcc gcaaacaact ccagtccgct ttttcccgga
     3061 ttcacacctc aaggatcgag ctcgtgaatt tcccgcccgc ctttgtgtat tacagccgaa
     3121 gataacccga tcctcccctt ccacctcgtt actgtgccaa gtcgtatcca gcgccagtca
     3181 accaccccca ccaccgccgc cgcccgcatc aaggggccga aggaagtttc cgtcatggcg
     3241 tggatcgaaa atcaattcgg cgaggtgctg ctgctcaagc agtcgaatgg ccggcggctc
     3301 tggacgctgc cgggcggcaa ggtccggacg cgcgaggcgc tggccacggc actgcgccgg
     3361 gaagtgcttg aggaaaccgg gcttcagctc aagtcgctgg aattcgccca cctttttgac
     3421 cgtccccgca aaagcacgat cacctttgtg tatcgcgtca cgctgcgggg aaaggatacg
     3481 gtggttccga aggcgaacga aatcgagacc gcccaatact gggccacgct gccccgaagc
     3541 gccacgccct ccctggcgtt tttctggaag cagcggcgcg gatagcgccg tttcggttaa
     3601 acccgaaaac ggattttgaa taaaccacgg aggacacgga gagaaggggc tggggaagaa
     3661 gccacgggat tttttcattc tacttactct ctgtgctcac tgtggtttat tcgctatttt
     3721 tgggtttaga gcgattctca aaagagattt ccgaaaaggg atccaatgta ggggcagccg
     3781 gagcgaagac agcctg
//
LOCUS       DATDAY010000388         2007 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_210927, whole genome shotgun sequence.
ACCESSION   DATDAY010000388 DATDAY010000000
VERSION     DATDAY010000388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2007
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_210927"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..657)
                     /locus_tag="VIS74_07605"
     CDS             complement(<1..657)
                     /locus_tag="VIS74_07605"
                     /inference="COORDINATES: protein motif:HMM:NF041881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5793148.1"
                     /translation="MVPLSAPADTLTLKSGEVLKGEILRETPESIVINNQFSPNIVEE
                     RTVARADIEKIEREQADEKAFGEIAAIPLPATAFDAEVYNEVINGQLEPFLKKYPYSI
                     HAEAVRAKIAEFQAEKKRLSEGRIKLEGRWVMRAQIEEETYQLDAQRAYEVIERQFLK
                     YNLVGALNQFDAFETKWPYSTSYPDAVEMARRMLGVLNQVLTHQLRNFPIKEQRRQLA
                     L"
     gene            complement(744..1460)
                     /locus_tag="VIS74_07610"
     CDS             complement(744..1460)
                     /locus_tag="VIS74_07610"
                     /inference="COORDINATES: protein motif:HMM:NF016817.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallocarboxypeptidase"
                     /protein_id="HEY5793149.1"
                     /translation="MIERWRALAARWGWQLYAFAQESGQEIFVIESAFPAGPSLYLSA
                     GIHGDEPASTEGLLAWAEGSAERLSRCAFTLFPCLNPWGLILNMRNDSQGRDLNRSYN
                     DPECQQTLRQLAWIADRSYDAAFMLHEDYDGTGYYLYEITPGESWGGQLVAAAAPFVG
                     VEGRPDIEGVPHSGGVVTRELAPDLLELMPKHPEALFLAVNGTTRCYTLETPSESAID
                     CRVDAHVAVLNRALDLLGAA"
     gene            complement(1490..>2007)
                     /locus_tag="VIS74_07615"
     CDS             complement(1490..>2007)
                     /locus_tag="VIS74_07615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HEY5793150.1"
                     /translation="PLFYEKFYFTPGDLGFRTWDTKFGKIGVCVCWDQWYPEAARLTA
                     LRGAEILFYPTAIGWHPSEKAEYGERQHSSWETIQRSHAIANGCYVVAVNRVGFENPV
                     GGDGLEFWGQSFVADPAGQVMARASVANEESLVVEVDLAEVEFQRTHWPFLRDRRVDA
                     YADISRRFIDE"
BASE COUNT          401 a          670 c          556 g          380 t
ORIGIN      
        1 aagcgcgagc tggcgccgct gctccttgat cgggaagtta cgcaattggt gggtcagcac
       61 ctgattgagc accccaagca tccgccgggc catttccacc gcgtccggat acgacgtgga
      121 gtaaggccac ttggtttcaa aggcgtcaaa ttggttgagc gcgccgacca aattgtattt
      181 caaaaactgg cgctcgatca cctcatacgc ccgctgggca tcgagttggt aggtttcctc
      241 ctcgatctgc gcccgcatga cccaacgccc ctccaacttg atccgcccct cggacaagcg
      301 ctttttttcc gcctgaaatt ccgcgatctt tgcgcgaacc gcctccgcat gaatggagta
      361 gggatacttc ttcaaaaacg gttccagttg tccgttaatg acttcgttgt aaacttccgc
      421 atcaaacgcg gtcgccggaa gcgggatcgc cgcaatttcc ccgaaggcct tctcgtccgc
      481 ctgctcacgt tcgatcttct cgatgtccgc ccgcgcgacg gtgcgttcct ccacgatatt
      541 gggactgaac tggttattga tcacgatcga ctccggcgtc tcgcgcagaa tctcaccctt
      601 taatacctcg cccgacttga gcgttaaggt gtccgccggg gctgacagcg ggaccaaggc
      661 caacaatatg caaagaattc cgggggtaac tttcattgaa actatctttc gcagcctaat
      721 cggccaattg gggaagtaaa tctctaagcc gcccccagga ggtcgagcgc ccggtttagc
      781 accgccacgt gcgcgtccac ccggcaatca atcgctgact ccgagggggt ttccagggta
      841 taacaccgcg tggttccgtt cacggccaga aaaagcgcct cgggatgttt cggcatcagt
      901 tccaataaat ccggcgccag ctcccgcgtc accaccccgc cggaatgcgg aacgccttcg
      961 atgtccggac ggccctccac ccccacaaac ggagccgccg ccgccacgag ctggccgccc
     1021 cagctctccc cgggggtgat ttcataaagg taatagccgg tgccgtcgta atcctcgtgc
     1081 agcatgaagg cggcatcata actccgatcg gcgatccacg ccagctggcg cagggtctgc
     1141 tggcattcgg gatcattgta actgcggttg aggtcgcgcc cctggctgtc gttgcgcatg
     1201 ttcaggatca atccccaggg attcaggcag ggaaaaagcg tgaacgcgca gcggctgagc
     1261 cgctcggcgc tgccttccgc ccaggccagg agcccctcgg tcgaagccgg ctcgtccccg
     1321 tgaatgcccg ccgacaaata cagggacggc ccggccggga acgcggactc aatgacaaaa
     1381 atctcctggc cgctttcctg cgcgaaggca tagagctgcc agccccacct cgccgcaagc
     1441 gcgcgccagc gttcaatcaa ataggggtaa tcgtgactcc gccccgccct cattcatcaa
     1501 taaaccggcg gctgatatcg gcgtaggcat ccacgcggcg gtcgcgcagg aacggccaat
     1561 gggtgcgctg gaactccacc tcggccaggt ccacctcgac caccagcgac tcctcattgg
     1621 cgacggacgc ccgcgccata acctgcccgg cggggtccgc cacgaagctc tggccccaga
     1681 attccagccc gtcgccgccg accggattct caaaacccac gcggttcacc gcgacgacgt
     1741 aacagccgtt ggcaatcgcg tggctgcgct ggatcgtctc ccacgagctg tgctggcgct
     1801 cgccatattc cgccttttcg gagggatgcc agccgatggc cgtcggataa aacagaatct
     1861 ccgcgccgcg cagcgccgtc aggcgcgccg cctcgggata ccattgatcc cagcagacgc
     1921 agacgccgat cttgccgaac ttcgtgtccc aggtccggaa cccgagatcg ccgggcgtga
     1981 aataaaattt ttcgtaaaac agcgggt
//
LOCUS       DATDAY010000389         2076 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_2536821, whole genome shotgun sequence.
ACCESSION   DATDAY010000389 DATDAY010000000
VERSION     DATDAY010000389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2076)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2076)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2076
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2536821"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..319
                     /locus_tag="VIS74_07620"
     CDS             <1..319
                     /locus_tag="VIS74_07620"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEY5793151.1"
                     /translation="GTGSRAKMDAMEVAGRTGTAQFWRMEENHKVRDSLAWFAGFAPF
                     KKPRYAFAVLVCGGDTGGRVSAPLAKEILRRLESKEPFTPEPVAPAQGHLRPIPMIPA
                     SDT"
     gene            480..749
                     /locus_tag="VIS74_07625"
     CDS             480..749
                     /locus_tag="VIS74_07625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5793152.1"
                     /translation="MDSRLTPPDTKASPNFGVSTKRITPLLGLSGKIDEAARAAAVPG
                     IILQPLVDNPVRAAIFPAWEMAVNPDKYRQDGQVNLAGNSKPLNS"
     gene            1100..1747
                     /locus_tag="VIS74_07630"
     CDS             1100..1747
                     /locus_tag="VIS74_07630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5793153.1"
                     /translation="MIGYEATANAEPLSSLDIGATGVAGSVSESGGVYTIKGSGADIW
                     GSSDQFYFLYQKMQGDFEVVARVQSIANTNTWAKAGLMMRETLNANSKNAFSMVSPGG
                     TRFQARAQTGGSSVTSGNGTTAPKWIRLVRAGNVFTGYFSSDDGTTWVQSGSATISMA
                     QTIYVGLAVTSHVNSVLTTAVIDQVDLPGQSGTLIQLGMAGEISAPAFTERGSDR"
     gene            1767..>2076
                     /locus_tag="VIS74_07635"
     CDS             1767..>2076
                     /locus_tag="VIS74_07635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5793154.1"
                     /translation="MNQRRGWHSLSGVALAGFLAFLPGRDAGAQESGPLQVGFETAEG
                     FAPGPLNSFGQWTASGVQVVNTGAFSGTQALGFLDDSGPGRIKLTLGTPLAGGTTFVD
                     F"
BASE COUNT          460 a          541 c          670 g          405 t
ORIGIN      
        1 gggcaccggc agccgggcga aaatggacgc catggaagtg gctgggcgga cgggcaccgc
       61 tcaattctgg cgcatggaag aaaaccataa ggtgcgcgac tccctggctt ggttcgcggg
      121 atttgctccg tttaagaaac cgcgttatgc ttttgccgtc ctggtgtgcg gaggcgacac
      181 tggcggacgg gtttccgcgc cactggcaaa ggaaatattg cgccggcttg agtcgaagga
      241 accattcacg cccgagccgg tcgcccccgc ccaagggcat ctccgtccga ttcccatgat
      301 tcccgcttcg gatacgtaag ggaacctcat cctttcacga aagccccggc ggcttcggcg
      361 aagccgccct gccaatggtg gatggggacg gctcgtggaa gggtgagggg ggccttctcc
      421 cctcatgccg tctgtgattt ttccgctagc ttcggcggag aaatcggatc gcttcggcaa
      481 tggactcgcg gctgactccg ccggacacaa aagcctctcc caatttcggc gtcagcacga
      541 agcggatcac cccgcttttg ggattatccg gaaagattga cgaagcggcg cgggccgcgg
      601 cggtgccggg catcatcctt caaccgctgg tggataatcc cgttagggcc gccatttttc
      661 cggcatggga aatggctgtg aaccctgata aatacaggca agacggccaa gtcaaccttg
      721 ctgggaatag caagccgctt aactcttgaa aaaataatgt aacaaaccac ctggtgggct
      781 tgacgattag attcttatat ccatactccg ccaaaactct caggaaagag cgaaaacagc
      841 ggcgataaga actacccgat gaattcaaaa cccccggaac attccatgaa acagagcttt
      901 ttcggaaagg caatctccgc ctgacagtcc aggcgtcaat ttagcgcaaa aaagtataac
      961 aatatacctg acggaaatgg atgaacttat gaaatcggca aatcagtcat caagcatcaa
     1021 gaggtggggg acttgcgcct cgggccggtc cgcgaaggtt tctgcgattg gcagggtaat
     1081 cgcgatagtg ttcgccggaa tgattggtta tgaggcgacg gcaaatgcgg agccattgag
     1141 ctcgctggac atcggggcca cgggggttgc gggaagcgtg tcggagagcg gaggggttta
     1201 cacgatcaag ggaagcgggg ccgacatctg ggggtcgagc gaccagtttt atttcctgta
     1261 tcaaaaaatg cagggggatt ttgaggtggt ggcccgggtg cagagcatcg cgaataccaa
     1321 cacatgggcc aaggcaggtt tgatgatgcg ggaaactttg aatgccaatt ccaagaacgc
     1381 cttcagcatg gtgagccctg gaggaacccg atttcaggcg cgggcacaga ccgggggcag
     1441 cagcgtcacg agcggcaatg gcaccacggc gcccaagtgg atccgcctgg tgagggcggg
     1501 aaacgtcttc accggctact tctcatctga tgacggcacg acgtgggtgc aatcaggcag
     1561 cgcgaccatt tccatggcgc agaccatcta tgtgggcctg gccgtgacct cgcacgtaaa
     1621 ctcggtattg acgacagcgg ttatcgacca agtcgatttg cctggacaga gcgggacgct
     1681 gatccagttg gggatggcgg gagaaatttc ggctccggca ttcacggagc gcggatcgga
     1741 ccggtgatga caccagagag cgggctatga accaacggcg agggtggcac agcttgagcg
     1801 gtgtagcgct ggcgggattc ctggcgtttt tgccgggccg ggatgccggg gcgcaggaaa
     1861 gcggcccgct acaggtcggt ttcgagacgg cggaaggctt tgctcccggc ccgctgaatt
     1921 cattcggcca atggacggcg agcggagtgc aagtcgtcaa tacgggcgcg ttttcgggca
     1981 cccaggcatt ggggtttttg gacgactcgg gtcctggccg gatcaagttg acgttgggga
     2041 cgccgctggc agggggaacg acctttgtgg attttt
//
LOCUS       DATDAY010000390         4981 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_678598, whole genome shotgun sequence.
ACCESSION   DATDAY010000390 DATDAY010000000
VERSION     DATDAY010000390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 4981)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4981)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4981
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_678598"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            189..617
                     /locus_tag="VIS74_07640"
     CDS             189..617
                     /locus_tag="VIS74_07640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Minf_1886 family protein"
                     /protein_id="HEY5793155.1"
                     /translation="MPSALTFNEAVDLLVQEDSRYDAEAYTFLRDALETTLKKRKKSK
                     KETAGSHVSADELLDGFRLHALKEFGPMTPTVLGYWGVRTCEDVGHLVFNLVKAGIFA
                     KTDQDTMDAFRAGFEFEAAFVTPFRPTGKLLSAGGAGGVG"
     gene            651..3242
                     /locus_tag="VIS74_07645"
     CDS             651..3242
                     /locus_tag="VIS74_07645"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEY5793156.1"
                     /translation="MVVTTALLQAKEVPVTAAPSLPESKAQIFTLPNGLTVIIEEDHS
                     APVASVQVWCATGSIHEGKWMGAGLSHILEHMLFKGTPARGGGQIARQIQEQGGYINA
                     YTSYDRTVYWIDVPSSGVSQAVEILSDAMMNSTLPEDEYTKEQEVIRREYAMGFDDPN
                     RMGIQLLMRTVFGESPYQHPIIGYLDVYNKLTRQDVLDYYKSRYVPNNLTFVISGDVD
                     AAKLKTQLETYFEKYPRAPLPPVFIPTEPEQMGRREVNQEFPTELTRLYLAWRVPGMD
                     HPDAAALEILGQILGAGRSSILNLDIREKQQLAHSISAGVFSMPGQGVFFVGAVTDPD
                     KRAAVETEALKLIEQLKARGVTAEEVEKSRRNLLSSQLSALTTARGKASDLGSNWQLT
                     RNLDFGREFLRQVNAVTPADVRRVAQRYLVEDTLTLTSLNPIGSLAQSRPEETKTEAA
                     AVQKFELSNGLRLLVREDSRLPLVDVTAVFRGGLLAETAADNGITQLLSRTLLKGTQT
                     RSAEQIASQIESVGGSIGSDAGNNSFNVSVEVMKPDLKLGIELLADVLQHPTFPKTEV
                     ELEKKSQIASIKADEDQPTTIARNALRERLFGSHPYALRSKGTAQTVASLTPEQLREL
                     HRELVTAKNGVIAVFGDVKAAEVKALIEKNLGSLPAGQLALTQPPQPEKIVEKAEITE
                     ERNKQQAVIMVGYQTANALSPDRPALELIDEACSDLGSRFFDRIREQQALAYFVGSSQ
                     MMGLAPGLFAFYAGTDPKKADYAKSELVKEIAELAKTGLTSEELARAKKKLLGSEAIS
                     NQSNSSFAQACAINELQGLGYDYYQKREAEIQGITLADTQRVASKYFANPPVEVMVRP
                     PAAPKTN"
     gene            3248..3532
                     /locus_tag="VIS74_07650"
     CDS             3248..3532
                     /locus_tag="VIS74_07650"
                     /inference="COORDINATES: protein motif:HMM:NF020473.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1844 domain-containing protein"
                     /protein_id="HEY5793157.1"
                     /translation="MAEVQRNRVNAGETTQRFISFLIRQSQQAALCLGQIPHPQTGKA
                     EAHPEIAKLFIEDLEMIREKTRGNLSGEEIKILDDILANLQCEYAKVVSQ"
     gene            complement(3849..4379)
                     /locus_tag="VIS74_07655"
     CDS             complement(3849..4379)
                     /locus_tag="VIS74_07655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6036 family nucleotidyltransferase"
                     /protein_id="HEY5793158.1"
                     /translation="MRLRSLIHLVEAASALGQSRKILVMGSSALLGSFPELGEAGGPL
                     ETTYDADLLPDPINSEIAGFLLESLGENSLFHEAQGYHLDVLAPEIVEALPAGWQDRL
                     VPLNSRADCLDPCDVAAVKVRVGREKDVFVVRTLLELGKIQENALRERIASLELGERE
                     LFRTSRNLSVILRRDS"
     gene            complement(4361..4681)
                     /locus_tag="VIS74_07660"
     CDS             complement(4361..4681)
                     /locus_tag="VIS74_07660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5793159.1"
                     /translation="MPDRFDRMGMYRLIAQKIRRNPELLDIALANIERWLAKGQDQPH
                     RLREWRDRIREAQADEAGLRALLELLQEDSERADYLRGFAPFAGILSTQERRPFIREC
                     AYVH"
     gene            complement(4715..>4981)
                     /locus_tag="VIS74_07665"
     CDS             complement(4715..>4981)
                     /locus_tag="VIS74_07665"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEY5793160.1"
                     /translation="PFAIAVALHHGVVTCGNIGLVAQRDATIIGDTVNTAFRLEGVMK
                     ELGVSCLCSESFAKTFPHSHGFRDLGPRLLKGKSKPVRVFEVMS"
BASE COUNT         1072 a         1616 c         1378 g          915 t
ORIGIN      
        1 agttaccagt cacgccctca cccaacttcc ccattgtccc ctccgattcg cttgctaagg
       61 tagtgccgtg cttacccgcc gggaaaaaag aatcattgcg ttcgtctttc tggcgttttt
      121 agttggttgg tccgtgctgg aatggaaatc ccgcttttcc accggcgccc cagctcccac
      181 aatcgaacat gccatccgct ctgaccttta acgaagccgt ggaccttttg gttcaggaag
      241 actcgcgcta tgatgccgag gcttacacct tcctgcgcga tgcgttggaa acgaccctga
      301 aaaagcgcaa gaaatccaaa aaggaaaccg ccggctcgca cgtcagcgcg gatgaattgc
      361 tggatggctt ccggctgcac gccctcaagg agttcggtcc catgaccccg accgtcctcg
      421 gttactgggg tgtccgcacc tgcgaggatg tcggccattt ggtcttcaat ctcgtgaagg
      481 ccggcatttt cgccaagacc gaccaggaca ccatggatgc ctttcgcgcc ggtttcgagt
      541 tcgaggcggc tttcgtcacc cccttccggc ccaccggtaa attattgagc gccggaggcg
      601 cgggtggtgt cggataagat tcaatgattc gtaatttcat tccccttttc atggttgtta
      661 ccaccgctct gctccaggcc aaggaagtcc ccgtcaccgc cgccccctcc ctgcccgaga
      721 gcaaggccca gattttcacc ctgccaaacg ggcttacggt catcatcgag gaggatcaca
      781 gcgccccggt cgccagcgtc caggtgtggt gcgccaccgg cagcatccat gaggggaaat
      841 ggatgggcgc cggcctttcg cacatcctcg agcacatgct cttcaagggc acgccggccc
      901 gcggcggcgg ccagatcgcc cgccaaatcc aggaacaggg cggctacatc aatgcctaca
      961 cgtcctatga tcgcacggtg tattggattg atgtccccag ctccggcgtt tcccaggccg
     1021 tcgagatttt gtcggacgcg atgatgaact ccaccctgcc ggaggacgaa tacacgaagg
     1081 agcaggaggt catccgccgc gaatacgcga tggggttcga cgatcccaac cgcatgggca
     1141 tccagctcct catgcgcacg gtgttcggcg agagccccta ccagcatccg atcatcggct
     1201 acctcgacgt ttacaacaag ctcacccgcc aggatgtgct ggattattac aagtcgcgtt
     1261 acgttcccaa caacctcacc ttcgtcatct cgggtgacgt ggacgccgcg aagctcaaaa
     1321 cccagctgga aacctatttt gagaaatatc cgcgcgcgcc gctgccgccg gttttcattc
     1381 ccaccgaacc cgagcaaatg ggccgccggg aggtcaatca ggaattcccc accgaactca
     1441 cccgccttta tctggcctgg cgcgttcccg gcatggacca tcccgacgcc gccgcgctgg
     1501 aaatcctcgg ccagatcctc ggcgccgggc gcagctccat tctgaatctc gacatccgcg
     1561 aaaagcagca gctcgcgcac tccatttccg ccggcgtgtt ctccatgccc ggacagggcg
     1621 tattcttcgt cggcgcggtc accgatcccg acaagcgggc ggcggtcgaa acggaagcgc
     1681 tcaagctgat cgagcagctc aaggcccgcg gagttaccgc ggaggaagtc gaaaagtccc
     1741 gccgcaatct cctttccagc cagctctccg ccctcaccac ggcccgcggc aaggcgtccg
     1801 acctcggctc gaactggcag ctcacccgca atctcgattt cggccgcgag tttctgcgcc
     1861 aggtcaatgc cgtgactccc gccgacgtcc ggcgcgtcgc gcaaagatac ctcgtcgagg
     1921 acacgctcac gctcacctcg ctgaacccca tcggttcgct ggcccagtcc cgcccggaag
     1981 aaacgaagac cgaggcggcg gcggttcaga aattcgagct ttccaacggg ctgcgcctgc
     2041 tcgtgcgcga agattcccgc ctgccgctcg tggacgtcac cgcggttttc cgcggcggcc
     2101 tgctcgcgga aacggcggcg gacaatggca tcacccagct cctgtcccgc acgctgctca
     2161 agggaaccca aacccgcagc gcggaacaga tcgccagcca gatcgagagc gtcggcggca
     2221 gcatcggcag cgacgcgggc aacaacagct ttaatgtctc cgtggaggtg atgaagcccg
     2281 atctcaagct cggcatcgaa ctgcttgccg atgtgctcca gcatcccacc tttccgaaaa
     2341 ccgaggtgga actggaaaaa aaatcgcaga tcgccagcat caaggccgac gaggaccagc
     2401 cgacgaccat cgcgcggaac gccctgcgcg aacggctctt cggcagccat ccctacgcgc
     2461 tccgcagcaa aggcaccgcg caaaccgtgg cgtcgctcac gccggaacag ctgcgggaac
     2521 tccaccgcga actcgtgacc gcgaagaatg gcgtcatcgc cgtcttcggc gacgtgaagg
     2581 ccgcggaggt caaggccctc atcgaaaaaa atctcggcag cctccccgcc ggccaattgg
     2641 cgctgacgca gccgccgcag cccgaaaaaa tcgtggaaaa ggcggagatc accgaagagc
     2701 gcaacaagca gcaggccgtc atcatggtcg gttaccaaac cgccaacgcc ctcagccccg
     2761 accgcccggc gctggagttg atcgatgaag cctgcagcga tctcggctcc cgcttcttcg
     2821 accgcatccg cgaacagcag gcgctcgcct attttgtcgg ctccagccag atgatgggac
     2881 tcgcgcccgg gttgttcgcg ttctacgcgg gcacggaccc gaaaaaggcc gattacgcca
     2941 agtccgagct ggtgaaggaa atcgccgaac tcgccaagac gggcctgact tccgaggaac
     3001 tggcccgcgc gaaaaagaag ctgctcgggt cggaagccat cagcaaccag agcaattcga
     3061 gcttcgcgca agcctgcgcg atcaacgagc tccaggggct gggctacgac tattatcaaa
     3121 aacgcgaggc ggaaatccaa ggcatcacgc tggccgacac gcagcgcgtc gcgtcgaaat
     3181 acttcgccaa tccgccggtg gaagtcatgg tccgcccgcc cgccgcgccg aaaacgaatt
     3241 aagcgtcatg gccgaggtgc agcggaatcg ggtcaatgcc ggggaaacaa ctcagcggtt
     3301 catttcgttc ctgatccgcc aatcccagca ggcggcgctc tgcctgggcc agatcccgca
     3361 tccgcagacc ggcaaagccg aggcgcatcc ggaaatcgcc aaacttttca tcgaggatct
     3421 ggaaatgatc cgcgaaaaaa cccgcggcaa cctcagcggc gaggaaatca aaatcctcga
     3481 cgacatcctc gcgaatttgc agtgcgagta tgccaaggtg gtttcccaat aaaggaacta
     3541 aacgggattg actggatttg ccggatttgc cggatttgat ttaaaaattt tcccgttaat
     3601 cccgccaatc cggtagaatt cggtttagag cgattctcaa aagagatttc cgaaataccc
     3661 aacgggttgt agaggcggtt gtcgggtggg gaaatggggc cccaccaccc cattgggcgc
     3721 ctttggttgc ggggaaacgg ggaccccgca tccccgttta cgctgggcgg ttggcacaac
     3781 cgcccctaca attgaatccc ttttcggaaa tctcttttga gaaatgcgat agttcctcaa
     3841 agcccttttc aggaatctct ccgcaaaata accgataagt tgcgggaggt tcgaaacagc
     3901 tcacgttctc ccaactccag gctcgcgatt cgttcccgca aggcattttc ctgaattttt
     3961 cccaattcca gcagcgtccg gaccacaaaa acatctttct cccggccaac ccgcaccttg
     4021 acggccgcca cgtcgcaggg atcgagacag tcggcccggg agttgagcgg caccaatcga
     4081 tcctgccatc ccgcgggcaa tgcttccacg atctccggcg caagaacgtc caaatgataa
     4141 ccctgcgcct catgaaaaag gctgttttcc cccaacgatt ccaaaagaaa tccggcgatc
     4201 tcggaattga tgggatccgg aagcaagtcc gcatcataag tggtttccaa tggaccgccc
     4261 gcttcgccca attcgggaaa acttcccagc aatgcggatg accccatgac gaggatttta
     4321 cggctctggc caagagccga cgcggcctcc acgagatgaa tcaatgaacg taagcgcatt
     4381 cgcgaatgaa cgggcgccgt tcctgggtgc ttaaaatgcc cgcaaaagga gcgaaccccc
     4441 tcaaatagtc cgcccgctcg ctgtcttcct gcagcaactc gagcagcgcc cgcaggccgg
     4501 cttcatccgc ctgcgcctcc cggatgcgat cccgccattc ccgcaggcga tgcggctggt
     4561 cctggccttt cgccagccac cgttcgatgt tggccagggc aatgtccaat agctccggat
     4621 tccgccttat tttttgggcg atcaaccgat acattcccat acggtcaaac cgatccggca
     4681 tgcctggttt cataccattg aacgaaaagc attgctagga cattacctca aacacgcgga
     4741 ccggtttcga ttttcccttg agcaggcgcg ggccgaggtc gcggaagccg tgggaatgcg
     4801 ggaaggtctt cgcgaaactt tccgagcaca ggcaggagac gccgagttcc ttcatcacgc
     4861 cttcgagccg gaaggcggtg ttcacggtgt cgccgatgat ggtggcgtcg cgctgggcga
     4921 cgaggccaat gttgccgcag gtgaccacgc cgtggtgaag cgcgacggca atggcaaagg
     4981 g
//
LOCUS       DATDAY010000391         5214 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_1738838, whole genome shotgun sequence.
ACCESSION   DATDAY010000391 DATDAY010000000
VERSION     DATDAY010000391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 5214)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5214)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5214
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1738838"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(127..2238)
                     /gene="ppk1"
                     /locus_tag="VIS74_07670"
     CDS             complement(127..2238)
                     /gene="ppk1"
                     /locus_tag="VIS74_07670"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:NF003921.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HEY5793161.1"
                     /translation="MNPHLTSSGAGTEHAPVNFDDPRYFINRELTWLEFNQRVLDQAL
                     DESNPLLERLKFLCIVSSNLDEFFEVRYAGLKQQKQLHASEVGPDGLTASEQLAAIST
                     RTRQMVEDQYRCWRETIRPELEANRIRFHKYPDVPEAEKAHFEEFFRQHVYPVLTPIA
                     IDPAHPFPQLLNKSLNVIVHLEGEDLSTDLAVVQVPRILPRVVPFKASQDGDDYVFLG
                     HVIQHHVGSLFKGVQVKGAHLFRITRNSNLYVDEEEAHNLLHAIEAELRRVNRGAAVR
                     LEVGDECPDSVVDRLIEVFNLEREDVFRINGPLNLVRLMPLALQIDRPELRYKRFTPV
                     TVVSLDSEADMFYHIRKGDILLHHPYDNFQTVVDFIGQAAEDPHVLAIKQTLYRTSSD
                     SPIVQSLIEAARNGKQVTAVIELKARFDEAANIKWARLMREAGVDVVYGVSGLKTHAK
                     AMLIVRSEGDHIRRYAHLGTGNYHPSTARIYTDLSLFTCRDEVTSELAEAFNLLTGVS
                     KYEPMKELMLAPHDLLSRLRELTENEIQHADAGRPASIYVKLNSLIEPSLVELLYRAS
                     CAGVKIRLLVRGMCALRPGIPEVSENIEVRSIVGRFLEHSRVYRFENGGEPKYYLASA
                     DWMPRNLFRRVELAFPVTSPGMREHIEEILAWFWKDNVKARRMRADGIYERIVSEEPP
                     FDCQAEFLADAARRRKSHLGT"
     gene            complement(2249..2899)
                     /locus_tag="VIS74_07675"
     CDS             complement(2249..2899)
                     /locus_tag="VIS74_07675"
                     /inference="COORDINATES: protein motif:HMM:NF015084.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5793162.1"
                     /translation="MISLGIWQDSERLDGPAHMARDEALLNEAEAPVLRIYRWPSRQV
                     SLGYFCSLREARVQYPGWPMVRRWTGGGVVEHGEDWTFSLAIPGARPPAGALYCEIHR
                     ALTEALRESGVNAVLRESNEPARPGGACFLNPVESDVMVEAVKIAGGAQRRNRRGVLH
                     QGSLRVAGVDSDFFERFGANLAPTTQPWTPSPAYLQAAGRLEQTRYATAEWLEKFK"
     gene            complement(2896..3513)
                     /gene="bioD"
                     /locus_tag="VIS74_07680"
     CDS             complement(2896..3513)
                     /gene="bioD"
                     /locus_tag="VIS74_07680"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: protein motif:HMM:NF024891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HEY5793163.1"
                     /translation="MRFFVTATDTEAGKTYVSALLARRLRAGGRNVAALKPISCGGRE
                     DAEALRDAADAGLTLEEINPVHFELPAAPLVAGKVDLVSLVRQIRGIDARVEALVVEG
                     IGGWLVPLTQAEFVEDLAIRLGYPVVVVAGCRLGAINHTLLTVRQVEQSGLPLAGVIL
                     NHFSAAPDSAGTGKALRSLLGGRFLGEIPHLAGELPEEIAARLGL"
     gene            complement(3517..4365)
                     /locus_tag="VIS74_07685"
     CDS             complement(3517..4365)
                     /locus_tag="VIS74_07685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY5793164.1"
                     /translation="MSTPAASLENLTKIYPVHLRKERVLAVRNLNLHVEAGQVYGLLG
                     PNGSGKSTSLKMLLGLVTPTSGTTRIFGKDSRNFDSKADVGFLPENPYFYKFLTAEET
                     LRFYGKICGMGGRKLRERIDELLALVGMEDARHRRVGGYSKGMLQRIGLAQALMQSPR
                     LLVLDEPTAGVDPVGSRQIRDLILDLKQRGITILLTSHLLEQVQQVCDRVGILSRGVL
                     VREGKLEDLVSIRDQTEYVVQGASPQLAAQIERLVKENGAALVDVRNPEQTLEKVFIE
                     ATAERS"
     gene            complement(4362..4643)
                     /locus_tag="VIS74_07690"
     CDS             complement(4362..4643)
                     /locus_tag="VIS74_07690"
                     /inference="COORDINATES: protein motif:HMM:NF012911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HEY5793165.1"
                     /translation="MSRIAKKVFYEGRVQGVGFRYTTRHLASGFEVTGTVKNLPDGRV
                     ELCVGGDKGEVEDFLRALRESTLAGHISREYAEDLSPETRWKGFTIVHA"
     gene            complement(4640..>5214)
                     /locus_tag="VIS74_07695"
     CDS             complement(4640..>5214)
                     /locus_tag="VIS74_07695"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HEY5793166.1"
                     /translation="GVCHLYVDAESDADMALRIAENGKCQRPGVCNSLETLLVHEAIA
                     AEYLPRLAAALAKVELRADEAARRILGERAQAATGETWWTEYLDYILSVKVVPTLEEA
                     VAHINHYGSHHSDAIVTRNEKTAQRFLNEVDSAAVYWNASTRFTDGGEFGFGAEIGIS
                     TDKLHARGPMGLPELTSYKYVIRGNGQIRE"
BASE COUNT          944 a         1704 c         1596 g          970 t
ORIGIN      
        1 gataggtctt gagataaagt tcccgggaaa ccttatcgag cttggcgtct tccgtggtga
       61 cagacattcg gaaagactat tcagcgaacc gcgcggctgc aattatttcg tgcgtcatcg
      121 gaatcactaa gtgccgagat ggcttttgcg ccggcgggcg gcatccgcca ggaactccgc
      181 ctggcagtcg aagggcggtt cctcggagac aatccgctcg taaatgccgt ccgcccgcat
      241 ccggcgcgcc ttcacgttgt ccttccagaa ccaggccagg atttcctcga tgtgctcgcg
      301 catgccgggg ctggtgaccg gaaaggccag ctcaacgcgg cggaagagat tgcgcggcat
      361 ccaatcggcg ctggcgaggt aatatttcgg ctccccgcca ttctcaaagc ggtaaacgcg
      421 gctgtgctcc aggaaacgcc cgacgatgct gcggacctcg atgttttcgc tgacttcggg
      481 aatgcccggc cgcagcgcgc acatgccgcg cacgagcagg cggatcttca ccccggcgca
      541 ggaggcgcgg taaagcagct cgaccaggct tggctcgatc agcgaattga gctttacgta
      601 aatgctggcc ggacggcccg catccgcgtg ctggatttca ttttcggtga gctcccgcag
      661 gcgcgacagc agatcgtgcg gcgcgagcat gagttccttc atcggctcgt atttggaaac
      721 tccggtcagc agattaaagg cctccgccag ctcgctggtg acctcgtccc ggcaggtgaa
      781 caggctcagg tcggtgtaga tgcgcgcggt ggagggatgg tagttgcccg tgccaaggtg
      841 ggcataccgg cggatgtgat ccccctcgga acgcacgatg agcatcgcct tcgcgtgcgt
      901 cttcaggccg ctgaccccgt aaaccacatc cacgccggcc tcgcgcatca ggcgggccca
      961 tttgatgttc gccgcctcgt caaaacgcgc cttgagctcg atgacggcgg tcacctgctt
     1021 gccattgcgc gcggcttcga tgagcgactg cacgatcggg gaatccgagc tcgtgcgata
     1081 cagcgtctgc ttgatcgcga ggacgtgcgg atcctccgcg gcctggccga tgaaatccac
     1141 gacggtctga aaattgtcgt agggatggtg cagcaggatg tcgcccttgc ggatgtggta
     1201 aaacatgtcc gcctcgctgt cgagggagac cacggtgacg ggcgtgaagc gcttgtagcg
     1261 gagctcgggc cggtcgatct gcagcgccag gggcatgagg cggacgagat tgagcgggcc
     1321 gttgatgcgg aagacgtcct cgcgctcgag gttgaacact tcgatgaggc gatccacgac
     1381 ggaatccggg cattcgtcgc ccacttccag gcggacggcc gccccgcggt tgacgcggcg
     1441 caattccgcc tcgatggcgt gcagcagatt gtgcgcctcc tcctcgtcca cgtagaggtt
     1501 gctgttgcgg gtaatccgga acaggtgagc tcccttgacc tgaaccccct tgaagaggct
     1561 gccgacatgg tgttggatca cgtggccgag gaacacgtag tcgtccccgt cctggctggc
     1621 cttgaacggc acgacgcgcg gcagaatgcg cgggacctgg acgacggcca gatcggtgct
     1681 caagtcctcg ccttccaggt gaacgatcac gttcaggctc ttgttcagca gctgcgggaa
     1741 gggatgcgcg ggatcgatgg cgatcggcgt cagcaccgga tacacgtgct gccggaaaaa
     1801 ctcctcaaaa tgggctttct ccgcctcggg gacgtcggga tatttatgaa agcggatgcg
     1861 attcgcctcg agttccggac gaatggtctc tcgccagcag cggtattgat cctccaccat
     1921 ctgccgggtg cgggtcgaga tggccgccaa ttgctcgctg gccgtcaagc catccggccc
     1981 cacttcggag gcatgcagct gcttctgctg cttgaggccg gcgtaacgca cctcgaaaaa
     2041 ttcgtcgagg ttcgagctga cgatgcagag aaatttcaac cgctccagca gcgggttgga
     2101 ttcgtccagc gcctggtcga gcacgcgttg gttgaattcc agccaggtca gctcgcgatt
     2161 gataaaatag cgcggatcgt caaagttcac cggggcatgc tccgtcccgg cgcccgagga
     2221 ggtcaagtga ggattcacaa agaaaacttc atttgaattt ctccagccac tccgcggtcg
     2281 cgtaacgcgt ttgttccagc cggccggcgg cttgcaaata ggcgggggaa ggggtccatg
     2341 gttgcgtggt tggagccaaa ttcgcgccaa aacgttcaaa aaaatcggaa tccacgccgg
     2401 ccactcgcag gctgccctgg tgcaagactc cgcggcgatt gcgccgctgg gcgcctccgg
     2461 cgatcttgac cgcttccacc atcacgtcgc tttccaccgg gttaaggaaa cacgcccccc
     2521 ccgggcgggc gggctcgttg gattcccgca ggacggcgtt cacgccggat tcccgcagcg
     2581 cctcggtcag cgcccggtga atctcgcaat aaagcgctcc cgccggcggg cgggcgcccg
     2641 gaatcgccaa ggaaaacgtc cagtcctccc cgtgttcgac cacgccgccc cccgtccagc
     2701 ggcgcaccat cggccagccc ggatattgga cccgcgcctc ccggagcgag caaaaataac
     2761 cgagcgaaac ctgccgcgaa ggccagcggt aaatccggag caccggcgcc tccgcctcgt
     2821 tcagcagcgc ttcgtcgcgc gccatgtggg ccgggccgtc gagccgctcc gaatcctgcc
     2881 agatgccgag cgaaatcata accccagccg cgcggcgatt tcctcgggca gctctccggc
     2941 cagatgcgga atttccccga gaaagcggcc gccaagcaac gaacgcagcg cctttccggt
     3001 gcccgccgaa tccggcgccg cgctgaaatg attgagaatc acgccggcca gcggcaagcc
     3061 gctttgttcg acctgccgca cggtcaagag cgtgtgattg atcgccccca gtcggcagcc
     3121 cgccacgacc acgaccggat agcctaggcg aatcgcgaga tcctccacga attccgcctg
     3181 agtcaaaggc accagccagc cgccgattcc ttcgacgacc agcgcttcaa cacgcgcgtc
     3241 gattccgcga atctgccgga ccagggatac cagatcgacc ttccccgcca ccaggggagc
     3301 ggctggcaat tcaaaatgaa cggggttgat ttcctccagc gtcagtcccg cgtccgcggc
     3361 atcccgcaac gcctcggcgt cctcccgtcc gccgcagctg atgggtttga gcgcggccac
     3421 gtttcgcccg cccgcgcgga ggcgccgggc cagcagcgcg gacacatagg tcttgccggc
     3481 ctccgtgtcc gtggcggtga caaaaaaacg cattcgtcag gaacgctcgg ccgtcgcttc
     3541 gatgaaaact ttttccagcg tctgttcggg gttgcggacg tccacgagcg cggcgccatt
     3601 ttccttcact aaacgctcga tctgcgccgc cagctgcggg ctcgcccctt ggacgacgta
     3661 ttcggtctga tcgcgaatcg aaacgaggtc ttccagcttg ccttcccgga ccaaaacccc
     3721 gcgcgaaaga atgcccaccc ggtcgcacac ttgctgcacc tgctcgagca agtgcgaggt
     3781 caggagaatg gtgatgccgc gctgtttgag atcgaggatc aggtcgcgaa tctgccgcga
     3841 acccaccggg tccacgccgg ccgtcggctc gtcgagcacc agcagccggg gcgactgcat
     3901 caacgcctgg gccaggccga tgcgctgcag catgcccttc gagtaaccgc cgacgcggcg
     3961 gtgccgggcg tcctccattc ccaccagcgc cagcaattca tcgatgcgct cgcgcaattt
     4021 ccggccgccc atgccgcaga tctttccgta gaagcgcagc gtttcctccg cggtcaggaa
     4081 tttgtaaaaa tagggatttt ccggcaggaa ccccacgtcg gctttgctgt cgaagttccg
     4141 gctgtccttg ccgaaaatcc gcgtggttcc cgaggtcggc gtgaccagcc cgaggagcat
     4201 cttgaggctc gtgcttttgc ccgagccatt agggccgagc agtccgtaaa cctggcccgc
     4261 ctcgacgtgg agattcaaat tgcgcaccgc cagcacccgc tccttccgca ggtgcacggg
     4321 ataaattttc gtcagatttt caagggatgc cgcgggggtg ctcatgcgtg aacaatggta
     4381 aagcccttcc atcgggtttc cgggctcaaa tcctcagcgt attcccggct gatgtgcccg
     4441 gcaagcgtgc tctcgcgcag ggcccggaga aaatcctcga cctcgccctt gtcgccgccc
     4501 acgcatagct ccacccggcc gtcgggcaga tttttgaccg ttcccgtgac ctcaaacccg
     4561 ctggccaggt ggcgggtcgt gtagcggaaa cccacgccct gcacccggcc ctcgtaaaac
     4621 acttttttgg cgatgcggct cattcgcgaa tctgtccgtt tccccggatc acgtatttgt
     4681 agctcgtcaa ttccggcagg cccatcggcc cgcgcgcgtg cagcttgtcg gtgctgatgc
     4741 cgatctccgc gccgaagccg aactccccgc catcggtaaa gcgcgtggac gcgttccaat
     4801 aaaccgccgc ggaatccacc tcgttgagaa accgctgcgc ggtcttttcg ttccgggtca
     4861 cgatggcgtc gctgtggtgc gaaccgtagt ggttgatgtg cgcgacggcc tcctccagcg
     4921 tgggcacgac tttcacggag agaatgtaat ccaggtattc cgtccaccag gtttccccgg
     4981 tggccgcctg cgcgcgctcg ccgagaatcc gccgcgccgc ctcgtcggcc cgcagttcca
     5041 ccttcgccag cgccgccgcg agccggggca ggtattccgc ggcgattgcc tcatgcacca
     5101 gcagcgtctc gagcgaattg cacacgccgg gccgctggca tttgccgttc tccgcgatgc
     5161 gcagcgccat gtccgcgtcg ctctcggcgt ccacgtaaag atggcagaca ccgt
//
LOCUS       DATDAY010000392         4276 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_855505, whole genome shotgun sequence.
ACCESSION   DATDAY010000392 DATDAY010000000
VERSION     DATDAY010000392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 4276)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4276)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4276
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_855505"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..536)
                     /locus_tag="VIS74_07700"
     CDS             complement(<1..536)
                     /locus_tag="VIS74_07700"
                     /inference="COORDINATES: protein motif:HMM:NF012970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEY5793167.1"
                     /translation="MRYWIFAIGFWALAATAGWAAESYIIVDNQTGQILAAKDSNEKR
                     QIASLTKIATAMVVLDAEEQRLINLGNEVEVPQQAVDTGGINQTGLQAGDVISIRDLL
                     YSALLASDNVAAATLAYHVGRRLPNPTGLDSTGNFVAYLNALARTLGMKRTLFLNPHG
                     IDNVEGELPFSCAADLARL"
     gene            complement(627..1334)
                     /locus_tag="VIS74_07705"
     CDS             complement(627..1334)
                     /locus_tag="VIS74_07705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5793168.1"
                     /translation="MSELDYLRPVHRKTSLREWILMAVILGMLAVIVSAIFLKQQGDA
                     RLVAGSRNLQQWGIALNLYLIDNDNQLPGVGGVPVAPEQTQAWYNSLPPYISGTPLAD
                     LPPGKRPRPGVPSPWIDPASRPVRVWDASQFYFEYGMNQALQPDSRARSFQIYEIDSP
                     AHVVFMAPVSEYAPAATPGRVVFRYGGNPGGPNASAFVLFCDGHVEKVTRARLVQAPA
                     SRLAASVAAGKLSWFEK"
     gene            complement(1327..2808)
                     /locus_tag="VIS74_07710"
     CDS             complement(1327..2808)
                     /locus_tag="VIS74_07710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5793169.1"
                     /translation="MHRKSILGLGVLSLLAGMILAGCGRPPEETPDVTILQTGRIRGN
                     AYPLTLKGISPLQHYPYLAGYVKKVRQEAQKNGAEVLLVDLGDSLEGSFASYATGGAN
                     MAAFFNALDYDAIVLGNLDNNVQPELLSQLKARVLNPFQDAAGQPATQGTQFAASLNK
                     GGIPIYLLANFYGNVSEREFPERFPTWFGTTAAQVQPVRDYEAVLAALGPRAAGGLTL
                     FSWMKFEPPGQPPQAFLDELTRLGVNAILAQRVYGGKMRDVWSDSGFHDWRPPVSENI
                     LRNNGGFVLARLDLKREGDSWRVLRQELLPMTANTAPADSEIVAAIGKFAGPIAKADR
                     VLAELPAAVGEADILQAYAAALTRIPGTQAVIYSPESVRSEWPAGELRASEVFNSLPW
                     TNPLVQLPLTPEQLQSVQKNSALRVWIEDAAPGAPVTVTTSKYFATLLAAQLRLPAGA
                     LKETAEASEFAYFIRFLQEGGGSGVIAPLPAGWSLLPSVPAHE"
     gene            2993..3967
                     /gene="tal"
                     /locus_tag="VIS74_07715"
     CDS             2993..3967
                     /gene="tal"
                     /locus_tag="VIS74_07715"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF009001.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HEY5793170.1"
                     /translation="MSATLTAATQLDQLKQFTTVVADTGDFESIKKYEPQDATTNPTL
                     IFKAVQLDEYKPLLAQAIAENKSSSLSGDALAKKIIDDLLILFGGKILEIVPGRVSTE
                     VDARLSFDTESTIEKARYLISLYEKKGIPRERILIKIASTWEGIKAAEVVQKEGINCN
                     LTLLFSLPQAVACAEANIRLISPFVGRIYDYYKATTGKEYVGAEDPGVQSVNKIYDYY
                     KKFGYSTQVMGASFRNTGEIVELAGCDLLTISPDLLEKLQQSEAPLARKLDPRIAAKS
                     GIEKIENTEKNFRWLLNEDPMATDKLADGIRKFAADIVKLEEIILKSL"
     gene            3982..>4276
                     /locus_tag="VIS74_07720"
     CDS             3982..>4276
                     /locus_tag="VIS74_07720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5793171.1"
                     /translation="MRVLLATALVAASLTGLQAKPSASEFTGALPLLLTSENVRDELA
                     VNSLQRAVLDSLRAEYKVAVRELVATQPQTPEARKIAETKLAALNRQYNVRALS"
BASE COUNT          877 a         1391 c         1259 g          749 t
ORIGIN      
        1 aggcgggcga gatcggcggc gcaggagaac ggcagttccc cctccacgtt gtcaatcccg
       61 tgcggattga ggaaaagcgt gcgtttcatg cccaaagtgc gcgccagggc gttcaggtaa
      121 gccacaaaat tacccgtgga atccaggccg gtggggtttg gcaggcgccg gccgacatgg
      181 taggccagcg tggcggcggc cacgttgtcc gaagccagca gcgcggagta aaggagatcg
      241 cgaatcgaaa tcacatcgcc cgcctgcaag cccgtttgat tgatgcctcc cgtatccacc
      301 gcctgctgcg gcacctccac ttcgttgccg agattgatca ggcgctgctc ctccgcatcg
      361 agcaccacca tggcggtggc gatcttggtc aggctggcga tctgccgctt ttcattgctg
      421 tccttggccg ccaggatctg gccggtttgg ttgtccacaa taatgtaact ctccgccgcc
      481 catccagccg tggcggcgag ggcccaaaag ccaatcgcaa aaatccagta acgcatgctt
      541 caaatccgtt gtgccggaga gtaatttacc cggacgcctt ggcaatgccc ttttgcggct
      601 ggccgcaaaa atccaaaagg gcggatttat ttttcaaacc aggacagctt tcccgccgcc
      661 accgaggccg ccagccggga agccggagcc tgcaccagcc gggcgcgcgt gactttttcc
      721 acgtggccgt cgcagaacag gacgaacgcg gacgcattcg ggccgcccgg attgcccccg
      781 taacgaaaaa ccacccggcc gggcgtggcg gccggcgcgt attcgctgac cggagccatg
      841 aacaccacgt gggccgggga atcaatctcg taaatctgga aactgcgcgc gcgcgagtcg
      901 ggctggaggg cctgattcat gccgtattca aaataaaact gcgaggcgtc ccagacccgg
      961 accggccggg aggcgggatc gatccacggg gacggcacgc cgggacgggg ccgcttgccg
     1021 ggcggcagat cggccagcgg cgtcccggaa atatagggcg gcagactgtt ataccatgcc
     1081 tgggtctgct ccggagccac cggcaccccg cctaccccgg gaagctggtt gtcattgtcg
     1141 atgagataca aattcagcgc gatgccccat tgctgcaggt tccgggaacc ggccaccagc
     1201 cgggcgtccc cctgctgctt caggaaaatg gcgctcacga tgaccgccag catccccaaa
     1261 atgaccgcca tgaggatcca ttcgcggagg gaggtcttgc gatggaccgg ccgcaggtag
     1321 tcgagttcac tcatgggcgg gaacggacgg caggagcgac cagccggcgg gcagcggcgc
     1381 gatgacgccc gaaccgccgc cctcctggag aaaccgaatg aaatacgcaa actccgaagc
     1441 ctccgcggtt tccttgagcg cgccggcggg aagccggagc tgggccgcca gcagcgtcgc
     1501 aaaatatttg gaagtcgtca ccgtgaccgg cgctcccggc gcggcatcct cgatccaaac
     1561 ccgcagcgcg gagtttttct ggacggactg gagctgctcc ggcgtgagcg ggagttggac
     1621 gaggggattg gtccacggca ggctgttgaa gacctcgctc gcgcgtagtt cccccgccgg
     1681 ccattcggag cggacggatt ccggggaata gatgaccgcc tgggtgccgg gaatccgggt
     1741 cagcgccgcc gcatacgctt gcaggatgtc ggcttcccca accgccgcgg gcaactcggc
     1801 cagcacgcgg tcggccttcg cgatggggcc ggcaaacttg ccgatggccg cgacgatttc
     1861 cgaatccgcc ggcgcggtat tcgccgtcat gggcagcagt tcctgccgca gcacgcgcca
     1921 ggaatcgcct tcccgcttga ggtccagccg cgcgaggaca aacccgccat tgttgcgcag
     1981 gatgttctcg gaaaccggcg gacgccagtc atggaaaccg ctgtcgctcc acacatcccg
     2041 cattttgccg ccgtaaaccc gctgcgcgag gatcgcattc accccgagcc gggttaactc
     2101 gtccagaaac gcctgcggag gctggcctgg cggctcgaat ttcatccagc taaacaaggt
     2161 gaggccgccc gcggcccggg ggcccagcgc ggcgagaacc gcctcgtaat cccgcaccgg
     2221 ctggacctgc gcggccgtgg ttccaaacca ggtggggaaa cgctcgggga attcgcgctc
     2281 gctgacattc ccgtaaaaat tcgcgagcag gtaaatggga atgccgccct tgttcaaact
     2341 cgcggcaaat tgcgtcccct gggtggcggg ctggccggcg gcgtcctgaa acggattcaa
     2401 gaccctggcc ttcagctggc tgagcagctc cggctgcacg ttattatcga gattccccag
     2461 aacgatggcg tcataatcca gcgcgttaaa gaaggcggcc atattggccc cgcccgtcgc
     2521 atagctggca aacgaacctt ccaggctgtc ccccaaatcc accagcagca cctcggcgcc
     2581 gtttttttgg gcttcctgcc gcaccttctt cacatatccg gcgaggtagg gatagtgctg
     2641 caatggcgag atgcccttca gcgtcagcgg atacgcattt ccccgaatcc ggccggtttg
     2701 caggatggtg acatccggtg tttcctccgg cgggcggccg cagccggcca aaatcatccc
     2761 cgccagcaac gagagcaccc ccagccccag aattgatttt cgatgcatca tatttccgcc
     2821 gcgtgtgcgg accattactc cgatctcccg gtgcttcgag caaaattgcg ccggaaatcc
     2881 tgtcgcggtg ccaaaatttc gccgcatttt ccctctggcc ggacataacg acgcaatatg
     2941 gttgattttc cggtccgctg gacgcaggtt aagccggaca ttgaatgaat ttatgagcgc
     3001 gacccttacc gcagccaccc aacttgacca actcaagcaa ttcaccaccg tcgttgccga
     3061 caccggggat tttgaatcca tcaagaaata cgaaccgcag gacgccacga ccaaccccac
     3121 gctgattttc aaagcggttc agctcgacga atacaagccg ctgctcgccc aggcgatcgc
     3181 ggaaaacaaa agctcctcgc tctccggcga cgcgctggcc aagaagatca tcgacgatct
     3241 cctcattctc ttcggcggca agattctgga aatcgtgccc ggccgcgtct cgaccgaagt
     3301 ggacgcccgc ctgagcttcg acaccgagag caccatcgaa aaggcgcgtt acctcatcag
     3361 cctctacgaa aagaaaggca tcccccgcga acgcattctc atcaaaatcg cctccacgtg
     3421 ggagggcatc aaggccgccg aagtcgtgca gaaggaaggc attaattgca atctgaccct
     3481 gctcttctcg ctgccccagg ccgtggcctg cgccgaggca aatattcggc tcatctcgcc
     3541 tttcgtcggc cgcatctacg actattacaa agcgacgacc ggcaaggaat acgtcggcgc
     3601 ggaggatccc ggcgttcagt ccgtcaataa aatctacgat tactacaaga aatttggtta
     3661 ttcgacccag gtgatgggcg ccagtttccg gaatacgggt gaaattgtcg aactcgccgg
     3721 ttgcgacctg ctgacgatca gtcccgacct gctcgaaaaa ctccagcaat ccgaagcgcc
     3781 gctcgcgcgc aagctcgatc cgcggatcgc ggcgaagtcg ggcatcgaaa aaatcgaaaa
     3841 caccgagaaa aacttccgct ggctgctcaa cgaggatccc atggcgacgg acaagctggc
     3901 cgacggcatc cgcaagttcg ccgccgacat cgtcaagctc gaggaaatca tcctcaagtc
     3961 cctctgatcc tcgccccatt catgcgcgtt ctcctcgcta ccgccctcgt ggccgccagc
     4021 ctcaccgggc tgcaggccaa accctcggcc tcggaattca ccggggcgct gccgttgttg
     4081 ctgacctccg aaaatgtccg cgacgaactc gcggtgaact ccctgcagcg ggcggtcttg
     4141 gattcgctgc gggcggaata caaggtggcg gtccgcgaat tggtggccac ccaaccccag
     4201 actcccgagg cccggaaaat cgccgaaacc aagctcgcgg cgctcaaccg gcaatacaac
     4261 gtccgcgcgc tttccg
//
LOCUS       DATDAY010000393         2959 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_2270510, whole genome shotgun sequence.
ACCESSION   DATDAY010000393 DATDAY010000000
VERSION     DATDAY010000393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2959)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2959)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2959
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2270510"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..918)
                     /locus_tag="VIS74_07725"
     CDS             complement(<1..918)
                     /locus_tag="VIS74_07725"
                     /EC_number="2.6.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LL-diaminopimelate aminotransferase"
                     /protein_id="HEY5793172.1"
                     /translation="MAQLNSNYLKLKAGYLFPEINRRVKAFIDANPEAAKKLIRCGIG
                     DVTEPLPEAVRNAMHRAVDEYATREGFHGYGPEQGYEFLRKVIAEKDYRARGLNVADD
                     EIFVSDGSKCDCGNILDIFGHANRIAVMDPVYPVYVDTNVMAGHTGAADESGVYGGLV
                     YLPCTAENGFVPAVPAEKADLIYLCYPNNPTGATATREQLEGWVNYALKHEAVILYDA
                     AYEAYIQDGSIPRSIYEIPGAEKCAIEFRSFSKNGGFTGVRCAFTVVPKALECATDTG
                     EKRPLHPLWNRRFSTKFNGVSYITQRGAEA"
     gene            complement(1087..1713)
                     /locus_tag="VIS74_07730"
     CDS             complement(1087..1713)
                     /locus_tag="VIS74_07730"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEY5793173.1"
                     /translation="MAYELPALTYPTNALEPSIDARTMEIHHDKHHAAYVTNLNKALE
                     AQPELAAKSIDDLIADLSAVPDAIRGPVRNNGGGHWNHSFFWTLLSAGKGGAPTGALA
                     EAITATFGSFDEFKAKFEAAGLGRFGSGWAWLIVKADKSLAIVSTPNQDNPLMKGVAE
                     TLGTPVIGVDVWEHAYYLNYQNRRPDYLKAIWNVIDWDKAAENYAAAL"
     gene            complement(1874..2584)
                     /locus_tag="VIS74_07735"
     CDS             complement(1874..2584)
                     /locus_tag="VIS74_07735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5793174.1"
                     /translation="MRPFLTLVAILIVWLSAASARAMEKEYILLSGGPSLIEWEKFKA
                     EPHDQWWGNFIRAARVRIQQLQAQFGPGARITWLVYRPAYARRSARQDKADLLGNITS
                     VRDKYGINLVWVNSGKDVIHYLNNGQPRHAVKIADFEYYGHSNRACFMLDYSNEIDSG
                     SKAWLHENELAAIRRGLFTKDALIKSWGCYTGESMSRKWRAATGKKMIGAIGKTDYAH
                     GHERGWIPSLSAGSRWGG"
     gene            2696..2769
                     /locus_tag="VIS74_07740"
     tRNA            2696..2769
                     /locus_tag="VIS74_07740"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2728..2730,aa:Gln,seq:ttg)
     gene            complement(2737..>2959)
                     /locus_tag="VIS74_07745"
     CDS             complement(2737..>2959)
                     /locus_tag="VIS74_07745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5793175.1"
                     /translation="GDAGSPFPRNQRRPMGWWDPISPPDKRLYNAFGISEASFKNCPN
                     PLLSGLAAGYALRLEKLAAWLPDMDSNHE"
BASE COUNT          560 a          851 c          928 g          620 t
ORIGIN      
        1 cgcctcggcg ccgcgctggg taatgtagga cacgccgttg aacttcgtgc tgaagcggcg
       61 gttccacagc ggatgcagcg gccgcttttc gccggtgtcc gtggcgcatt ccagcgcctt
      121 cgggacgacc gtgaacgcgc agcgcacgcc ggtgaagccg ccgttctttg aaaagctgcg
      181 gaattcgatc gcgcacttct ccgcgccggg aatttcgtag atcgaacgcg ggatcgagcc
      241 gtcctgaata taggcctcgt aggccgcatc ataaagaatc accgcctcgt gcttgagcgc
      301 gtaattcacc cagccctcga gctgctcccg cgtggcggtc gcgccggtcg ggttgttcgg
      361 ataacacagg taaatcaggt ccgccttttc cgccggaact gccgggacaa agccgttctc
      421 cgccgtgcac ggcagataca ccagcccgcc atacacgccg gattcgtccg ccgcgccggt
      481 gtggccggcc atgacgttgg tgtccacata gaccggataa accggatcca tgaccgcaat
      541 gcggttggcg tggccgaaga tgtcgaggat gttgccgcaa tcgcacttcg agccgtccga
      601 cacgaagatt tcatcatccg cgacgttcaa cccgcgggcg cgataatcct tttccgcaat
      661 caccttgcgc agaaattcgt aaccctgctc cgggccgtag ccatgaaacc cctcgcgcgt
      721 ggcgtattcg tccaccgccc ggtgcatcgc gttccgcacg gcctcgggaa ggggctccgt
      781 gacgtcgccg atgccgcagc ggatcaattt tttcgccgcc tccggattcg cgtcgatgaa
      841 ggctttcacg cgccggttga tttcgggaaa gagatagccg gccttgagtt tgaggtaatt
      901 cgaattgagt tgagccatgg gatttttgga gtcttaagtt ttaagtctta agtgggtaag
      961 aaatccaagg tggatcgctt caaactttaa acttaagact taaaactccc ggggtcaggc
     1021 cggggtaaaa aatcgggcgc cgcagcttgc gcgcggcgcc cgatcacagc atgccggata
     1081 gttggattac agggcggccg cgtaattttc ggcggctttg tcccagtcga tcacgttcca
     1141 gatcgccttg aggtaatcgg gacggcggtt ctggtagttg aggtaatagg cgtgctccca
     1201 cacgtccacg ccgatgaccg gagtgccgag ggtttccgcc acgcccttca tgagcgggtt
     1261 gtcctggttc ggagtcgaga cgatggcgag cgacttgtcg gccttgacga tgagccaggc
     1321 ccagccgctg ccgaagcggc ccaggccggc ggcttcaaac ttggccttga attcgtcgaa
     1381 gctgccgaag gtggccgtaa tggcttccgc cagcgcgccg gtcggagcgc cgcccttgcc
     1441 cgcgctcagc agcgtccaga agaaactgtg gttccaatgg ccgccgccgt tgttgcggac
     1501 cggcccgcgg atggcgtccg gcaccgcgga aagatcggcg atgaggtcat cgatcgactt
     1561 cgccgccagt tcgggctggg cctcgagcgc cttgttgaga ttggtcacat aggcggcgtg
     1621 gtgcttgtcg tggtgaatct ccatcgtgcg cgcgtcgatg gaaggttcaa gcgcgttggt
     1681 gggataagtc agggcaggaa gttcgtatgc catggtagtg aatgggatga ggttggatta
     1741 aaagactgcc cttccaccct aaaccagccg gggaagcgtg caagcgtgat gtttggtgag
     1801 cggcagtggc atcggcatcc tgccgatgcg ttttatttga ggatcttcgg catcttgccg
     1861 atgccacttt aagctacccg ccccagcggc tgcccgcgct caacgagggg atccagccgc
     1921 gttcatggcc gtgggcgtag tcggtcttgc caatggcccc gatcattttt ttgcccgtcg
     1981 ccgcgcgcca tttccggctc atgctctcgc cggtgtagca gccccaactc ttgatgagcg
     2041 cgtccttcgt aaacagcccg cggcggatgg ccgccagctc gttctcgtgg agccaggcct
     2101 tcgagccgct gtcgatttcg ttgctgtaat cgagcatgaa acacgcgcgg ttcgagtgcc
     2161 cgtaatattc aaaatcggca atcttcaccg catgccgcgg ctggccgttg ttcaggtaat
     2221 ggatgacatc ctttccggaa ttcacccaga cgagattgat gccgtatttg tcgcggacgg
     2281 aggtgatgtt gccgaggaga tccgctttgt cctgccgggc cgagcggcgg gcgtaggccg
     2341 ggcgatagac cagccaggtg atgcgggcgc cgggcccgaa ctgggcctgc aattgctgga
     2401 tccgcacccg ggcggcgcgg atgaagttgc cccaccattg gtcatgcggt tcggccttga
     2461 acttttccca ttcgatcagg gaagggccgc cgctgaggag gatgtattcc ttctccatcg
     2521 cccgggcgga ggcggcggag agccagacga tcaggatcgc gacgagcgtg agaaaagggc
     2581 gcataacgaa tgaagaatga aaaagcttgt catgggtgca atccaaaatc caccttggaa
     2641 tgcatccacc ttttatttgc ggtccgtcgt cggctgcggt aaaagtgacg cacattgcct
     2701 gatggtgtaa cggtaacaca gcgccctttg gaggcgttat tcatggttcg aatccatgtc
     2761 gggcagccat gccgccaatt tttcaaggcg aagcgcatat ccggcagcga gaccggatag
     2821 aagcggatta gggcaattct taaaagaagc ttccgaaata ccaaacgcat tgtagaggcg
     2881 cttgtcgggt ggggaaatgg ggtcccacca ccccattggg cgcctttggt tgcggggaaa
     2941 cggggacccc gcatccccg
//
LOCUS       DATDAY010000394         5929 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_2496126, whole genome shotgun sequence.
ACCESSION   DATDAY010000394 DATDAY010000000
VERSION     DATDAY010000394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 5929)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5929)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5929
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2496126"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1598
                     /locus_tag="VIS74_07750"
     CDS             <1..1598
                     /locus_tag="VIS74_07750"
                     /inference="COORDINATES: protein motif:HMM:NF012485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type II and III secretion system protein"
                     /protein_id="HEY5793176.1"
                     /translation="TTPIADKTITLQLNNVPLYEALRYVTELAGLKIKVEPYAVSLVP
                     ISDVSGDFITKEYRVPAGFIPPTAAADTTNPAVPTFGTLATSGTARLPGRADAKTYLE
                     SQNIPFPPGASATYIPAGSKLVVRNTLDNIDLIDAYISAQTGGTPTQVEIESRFVEIT
                     QNDLKELGFDWLVGPFAIGSSGVYGTGGSGGGDASQFPFQSGGSAIGSNSLTSGLRSG
                     SGASPDSAVTVNSLDALLAAASGSGLTSAAAPALFGLSGIFTNPQFQVVIRGLDQRKG
                     VDLMTAPKVTTKSGLKASVKIAREFIYPTEFDPPQIPQETDSATVTTGAGFGAVFQTP
                     GAITPSNPGGWTTQDLGVTLGVTPTIGPDNYTIDLEIEPEVVDFDGFINYGSPINAVG
                     YRPEIPGSDLVAIGTVFVPEIQVLTQNVINQPIFTIRKITTTVTIWDGQTVALGGLIR
                     EDVQKVQDKVPFLGDIPFAGRLFRSNVDQKIKRNLIVFVTAKLIDAQGQPLRREEEEE
                     EIVEPVGLPAELPKPQFPMKGGK"
     gene            1665..2267
                     /gene="coaE"
                     /locus_tag="VIS74_07755"
     CDS             1665..2267
                     /gene="coaE"
                     /locus_tag="VIS74_07755"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEY5793177.1"
                     /translation="MFIAGLTGGAGSGKSTFRKIFCECSAAAFLDVDEVVRQLLQADA
                     GVRQAIENQLSSRAYDSGGCPDRAWLRELIYHQPEARETLESILHPAVRIAWRRAVAE
                     SRKAGRSLIVEIPLLFEKNLEAGFDCSILVAATPQTQLQRLAERGLDSAVARRMLAAQ
                     LPNEEKIPRASHVIWNDGSLDFLHAQAERLAHQFHRIYGN"
     gene            2257..3582
                     /gene="rho"
                     /locus_tag="VIS74_07760"
     CDS             2257..3582
                     /gene="rho"
                     /locus_tag="VIS74_07760"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF006886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HEY5793178.1"
                     /translation="METENLSPEMEVAAPAQTPYQPGTIDFLALQNQTHAQLVEENLR
                     LGIRVRADSNRFHLTLDQVRFHLTQGSRVEAAGILEMQGEHGQLRWEKFSFKPCVDDV
                     HLNPDLVRKYQLQTGYRLTFTVRLPRERERGLMVNELLFIEDIPAAEWQPPTPFDKLT
                     PLFPDKRVMLEDVRSGNISTRAMDLVAPLGFGQRGLIAAPPRAGKTMMLKAITQAIRR
                     NHPDVQLILLLVDERPEEVTDFHRETDAHIFSSTFDEPVSRHVQICELVSERAQRLVE
                     LGKPVVILVDSLTRMARGYNNLQPNKGRAMSGGVDSRALLKPRKFFGSARNTEEGGSL
                     TILATALVETHSRMDDLIFEEFKGTGNMELHLDRSIAEQRVFPAIHIIKSGTRREELL
                     YHPDEYSRIALLRRQLSELPAGEAMEVLAKNLHRTKSNAELLLAGLKGF"
     gene            3585..4814
                     /locus_tag="VIS74_07765"
     CDS             3585..4814
                     /locus_tag="VIS74_07765"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing SpoIIE family protein
                     phosphatase"
                     /protein_id="HEY5793179.1"
                     /translation="MSAPAPHRLLKVYKGLVEVSALINSITDFRELLHAILNVAREVF
                     QTEAASLFIANEETGELELLLACNETGYQEPQIVVPSGRGVAGWVWQRHEPALIRDAY
                     ADPRFYPEADRQTGFHTRSILCVPLKRIGVLQLLNPASAEDSDLEALQAYAALTATAI
                     EKLRALRSEHEQALLQRDLKIAAEIQEELLAHAIPQDLPHLHCFNEPAQNVGGDFYFA
                     QCLPDGGGVFAIGDVSGKGIPAALLMSQILNSLEFVFHSSCHPAEALQKLNALLHPRI
                     IRGMFATLLLGRYQNGSVELASAGHCQPLLLRKGTATEITVSTTLPIGVLPSIEPRSL
                     TLPLPPGAILLAYTDGLSESRDPASDHFLGDDLKTLLSRPFANPRQVVDFLVDTERHH
                     RRSQPRADDLTILAFQG"
     gene            4904..5632
                     /locus_tag="VIS74_07770"
     CDS             4904..5632
                     /locus_tag="VIS74_07770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b subunit"
                     /protein_id="HEY5793180.1"
                     /translation="MIRQPATSALVPPPANPITAWIFSSIGKKTIVAVTGIFLVLFVI
                     GHMLGNLQIFLGQHAINAYAAKLQALGPILWIIRLGLLTIVGLHIGFTVSLITDNAAA
                     SPQKYAAGNRLPATLFVRTMRYTGFLLLAFIVFHLAHFTLGMVQPAAFDLHDPEHLHD
                     VYSMMILGFQNGPISVFYILSMGFLAFHLSHGIGSLFQTLGINNRKLRPVFAQGGQVI
                     AWILFAGFSVIPFAVLTGCLTLPK"
     gene            5639..>5929
                     /locus_tag="VIS74_07775"
     CDS             5639..>5929
                     /locus_tag="VIS74_07775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEY5793181.1"
                     /translation="MTLDAKIPAGPLEQKWSYFKGHTKLINPANKRKFNLIIVGSGLA
                     GASAAATLAEQGYNIKCFCYQDSPRRAHSIAAQGGINAAKNYQNDGDSVYRLF"
BASE COUNT         1175 a         1982 c         1575 g         1197 t
ORIGIN      
        1 ccactacgcc gatcgcggac aaaaccatca ctctccaact caataacgtg ccgctctacg
       61 aggcgctgcg ttatgttacc gagctcgccg gactcaaaat caaggtggaa ccctacgcgg
      121 tctcactggt tccgatctcc gatgtgtcgg gcgactttat aaccaaggaa taccgggttc
      181 ccgccgggtt cattccgccc acggcggcgg cagacaccac caacccggcc gtgcccacct
      241 tcggaacgct ggcaacatcc ggaactgcca ggctccccgg ccgcgccgac gccaaaacct
      301 acttggaatc ccagaacatt cccttccctc ccggagcttc ggcgacttac attcccgccg
      361 gcagcaagct ggtggttcgg aacacgctgg ataatatcga cctgatcgac gcctacattt
      421 ccgcccaaac cggcggcact cccactcagg tggaaatcga atcgcgtttc gtggagatca
      481 ctcaaaacga tctcaaggaa ctgggattcg actggctggt cggccccttt gccattggct
      541 ccagtggtgt ttatggcacc ggtggttcgg ggggggggga tgcctcccag tttcccttcc
      601 aaagtggagg aagcgccatt ggctccaata gtttaaccag cggattacgt tccggttcag
      661 gagcttctcc cgattccgcg gtcaccgtga acagcctgga tgcgttgctg gccgccgcct
      721 cgggctctgg cctgacctcg gccgccgcgc ccgccctttt tgggctttcg ggcattttta
      781 ccaatcccca attccaagtg gtcattcgcg ggctggatca gagaaaaggc gtggatctga
      841 tgacggcccc gaaagtcacc accaaaagcg gcctgaaagc ctcggtgaag atcgcccgcg
      901 aattcattta tcccaccgag tttgaccccc cgcagattcc tcaggaaacc gactcggcca
      961 cggtcactac cggtgccgga tttggagcgg tcttccaaac tcccggagcg attactccct
     1021 ccaaccccgg cggatggacc acgcaggatt tgggcgtcac tcttggggta accccgacca
     1081 ttgggccgga taattacacc attgatctgg aaatcgagcc ggaggtggtg gatttcgatg
     1141 gtttcatcaa ctacggcagc cccatcaatg cggtcggcta tcgtccggaa atcccgggca
     1201 gtgatctggt ggcgatcggc acggtttttg ttcccgaaat ccaggttctc acccaaaacg
     1261 tcatcaacca gcccatcttc actatccgga aaatcaccac caccgtcacc atttgggacg
     1321 gtcagacggt ggcgctgggc ggcttgattc gcgaagacgt gcagaaggtt caggataaag
     1381 tgcccttcct gggcgacatt ccgttcgccg gccggctctt ccgctccaat gtcgatcaga
     1441 agatcaaaag aaatctgatt gttttcgtca cggccaaact catcgatgcc caggggcagc
     1501 ctttgcggag ggaagaggag gaggaagaaa tcgtggaacc ggtgggattg ccggccgaac
     1561 ttccgaagcc gcaattcccg atgaagggcg gaaaataatt tcgcggctgc cgtttccaaa
     1621 ttgcggcccg attccccgcc gggccgcaca acttcattct taacttgttt atcgcgggac
     1681 tcaccggagg agcgggttct ggaaagtcca ctttccggaa aatcttctgt gaatgctccg
     1741 cggcggcttt cctcgacgtg gacgaagtcg tgcgccagct tctccaagcg gatgcggggg
     1801 tcaggcaagc catcgaaaat caactctcct cccgcgccta tgactcgggg gggtgccccg
     1861 accgggcttg gttaagggag ctgatttacc accagcccga agcgcgggaa accctcgaat
     1921 ccatccttca tcccgccgtt cgaatcgcct ggcggcgggc cgttgcagag tcccggaaag
     1981 ccggccgttc gctgatcgtg gaaattcccc tgttattcga aaaaaatctt gaggctggat
     2041 tcgattgctc cattctggtt gcggcaaccc cgcagactca actgcaacgc ctcgccgaac
     2101 gcggactcga ctccgccgtg gcccggcgaa tgctcgctgc ccaattgccc aacgaagaaa
     2161 aaattccccg cgccagccac gtgatttgga acgacgggtc cttggacttt cttcacgccc
     2221 aggctgaacg cctcgcccac caatttcacc gcatctatgg aaactgaaaa cctttcacct
     2281 gaaatggaag ttgccgctcc ggcgcaaacg ccctatcagc ctggaacgat tgacttcctc
     2341 gcgcttcaga accaaaccca cgcccagctg gtggaggaaa atctgcggct cggcatccgc
     2401 gttcgggccg actcgaaccg ctttcacctc acgctcgacc aggtccgctt ccatctcacg
     2461 cagggcagcc gcgtggaggc cgccggcatt ctggaaatgc agggggagca cgggcagctt
     2521 cgctgggaga aattcagttt caaaccctgc gtggatgacg tccacctcaa tcccgatctg
     2581 gtccggaaat accagcttca aaccggttac cgcctgacct ttaccgtccg cctcccccgg
     2641 gaacgcgaac gcgggctcat ggtgaatgag cttctgttca tcgaagacat tcccgccgcc
     2701 gaatggcagc cgccgacgcc gttcgacaaa ctcaccccgc tcttccccga caaacgcgtg
     2761 atgctggagg atgtccgcag cggcaatatc agcacccgcg ccatggatct ggtcgctccg
     2821 ctcggattcg gccagcgcgg cctcattgcg gccccgcccc gcgcgggaaa gaccatgatg
     2881 ttaaaagcca tcacccaggc catccgccgg aatcatccgg acgtccagct cattcttctg
     2941 ctggtggatg aacgccccga ggaggtcacg gacttccatc gcgaaacgga tgcccacatt
     3001 ttcagctcca ccttcgacga gcccgtcagt cgccacgtgc agatctgcga gctcgtctcc
     3061 gagcgggctc agcggctcgt cgagctcggc aagcccgtgg tgattctcgt ggacagcctc
     3121 acgcgcatgg ctcgcggcta caataatctc cagccgaaca agggccgggc gatgtccggc
     3181 ggcgtggatt cccgcgcgct cctgaagccg cgcaaatttt ttggatcggc ccgcaatacc
     3241 gaggaaggcg gcagcctgac catcctcgcc accgcgctgg tcgaaaccca cagccggatg
     3301 gatgacctga tcttcgagga attcaagggc accggcaaca tggagctgca cctcgaccgc
     3361 agcatcgccg aacaacgcgt gtttcccgcc attcacatca ttaaatccgg aacccggcgc
     3421 gaagaactgc tttatcaccc cgatgaatac tcgcgcatcg ccttgctgcg gcggcagctt
     3481 tcggaattgc cggccggcga ggccatggag gttctcgcca agaatctgca ccgcacgaaa
     3541 tccaacgcgg aactgctgct ggccggcctg aagggctttt aggaatgagc gcgccggcac
     3601 cccatcggct gcttaaggtt tacaagggcc tcgtcgaagt cagcgcgctc atcaattcga
     3661 tcaccgactt ccgggaattg ctgcacgcga ttctgaacgt cgcccgcgaa gtcttccaaa
     3721 ccgaggccgc cagcctgttc atcgccaatg aggaaacggg cgagctcgaa ctgctgctgg
     3781 cctgcaatga aaccggttat caggaaccgc aaatcgtggt gccctccggc cgcggcgtcg
     3841 ccggctgggt ctggcagcgg catgaacccg cgctgatccg cgatgcctac gcggaccccc
     3901 gtttttatcc cgaagcggat cgccagaccg gattccacac ccgctccatt ctctgcgtgc
     3961 cgctgaaacg gatcggagtt cttcaactgc tcaatcccgc ctcggccgaa gattccgacc
     4021 tggaagcgct gcaggcctac gcggccctga ccgccacggc gattgaaaaa ctccgggccc
     4081 tccgctcgga acacgaacag gcgctcctgc agcgcgatct caagatcgcc gccgagattc
     4141 aggaagaact gctcgcccac gcgatcccgc aggatctgcc ccacctccat tgtttcaatg
     4201 aaccggcgca gaatgtgggg ggcgactttt attttgccca atgcctcccg gacggcggag
     4261 gcgtcttcgc catcggcgac gtttccggaa aaggcatccc ggccgcgctc ctgatgtcgc
     4321 agattctcaa ctcgctggag tttgtgtttc actccagctg ccaccccgcc gaggcgctgc
     4381 aaaaactgaa tgccctcctg catccacgca tcattcgcgg aatgttcgcc accctgctgc
     4441 tgggtcgtta ccaaaatggc agcgtcgaac tggccagcgc gggccattgc cagccgctgc
     4501 tgctgcggaa gggaacggcc accgaaatca ccgtttccac caccctgccg attggcgtgc
     4561 tccccagcat cgaaccccgc tcgctaacgc tgcccctgcc gcccggcgcc atcctgctcg
     4621 cctacacgga tggcttgagc gaatcccgcg accccgcctc ggatcacttt ttgggcgacg
     4681 atctcaaaac cctcctttcg cggcccttcg ccaatccccg gcaggtcgtg gatttcctgg
     4741 tggacaccga gcggcaccac cgccgttccc agccgcgggc ggatgatctg accatcctcg
     4801 cctttcaggg atgactccat gaatcccctt tccccgcagg ttttccgcat tgacgctgga
     4861 ttgctcttgc ttcgggcaaa gtattagcta atctaataac tgcatgattc ggcaacctgc
     4921 cacctccgct ctcgtgccgc caccggccaa tcccatcacc gcctggattt tttcctccat
     4981 tggcaaaaaa accatcgtcg cagtcaccgg catctttctg gtgttgttcg tcatcggcca
     5041 catgctgggc aatctccaga tttttctcgg ccagcatgcg atcaatgcct acgccgccaa
     5101 gctccaggcg ctgggcccga ttctttggat catccggctg gggttattaa ccatcgtcgg
     5161 cctccatatt ggtttcaccg tttccctgat cacggacaac gccgccgcct cgccgcaaaa
     5221 atacgcggcg ggaaaccggc tccccgcgac cctctttgtc cggaccatgc gctacacggg
     5281 attcctcctg ctggctttca tcgttttcca cctcgcgcat ttcactttgg gaatggttca
     5341 gcccgccgct tttgatctcc atgaccccga gcatctgcac gacgtctatt ccatgatgat
     5401 tctgggattt cagaatgggc cgatcagcgt tttttacatt ctctccatgg gttttctcgc
     5461 ctttcacctc agccacggga ttggcagcct tttccagaca cttggaatca acaaccgcaa
     5521 gctgcgcccc gtttttgccc agggcgggca ggtgatcgcg tggatccttt tcgccggttt
     5581 ttcggtgatc cccttcgccg ttctcacggg ctgcctcacc cttccgaaat aacctgccat
     5641 gacgctcgac gccaagattc ccgccggtcc gctggagcaa aaatggtcct acttcaaggg
     5701 ccacacgaag ctcatcaacc ccgccaacaa acggaagttc aacctcatca tcgtgggctc
     5761 cgggctggcc ggcgcctccg ccgcggcgac gctcgccgag cagggctaca acatcaaatg
     5821 tttctgctac caggacagcc cccgccgcgc ccatagcatc gccgcgcagg gtggcatcaa
     5881 cgccgcgaag aattaccaga acgacggcga cagcgtctat cgcctcttc
//
LOCUS       DATDAY010000395         2111 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_1239747, whole genome shotgun sequence.
ACCESSION   DATDAY010000395 DATDAY010000000
VERSION     DATDAY010000395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2111)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2111)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2111
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1239747"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..318
                     /locus_tag="VIS74_07780"
     CDS             <1..318
                     /locus_tag="VIS74_07780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HEY5793182.1"
                     /translation="VSVKGWTEESSLTALDLARRAEDAGAGTIIYTDIATDGMLEGPN
                     FAELEKMLGLLDCQLIASGGVSSAGDLRRLARLPDLYGAIIGKALYDGRITGNLREQI
                     VLD"
     gene            399..764
                     /locus_tag="VIS74_07785"
     CDS             399..764
                     /locus_tag="VIS74_07785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5793183.1"
                     /translation="MVVIAACSSTGSSTARSENNRYGRGSTGGQPTDTQFIEATPTPA
                     PSPTETAAPEPATVGGLPTPTPAPVVARDLPYGTPVPGKPGFVTSPHDPTAGYVDVRG
                     FAPGQEVRCPYTNKIFLVP"
     gene            776..1606
                     /locus_tag="VIS74_07790"
     CDS             776..1606
                     /locus_tag="VIS74_07790"
                     /inference="COORDINATES: protein motif:HMM:NF014234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HEY5793184.1"
                     /translation="MFAPGLIGASLGLALADRWRVRVWSRSESSAQAAARRIGAEFAS
                     ADPVETAAGADGIVFCSPIDAMPALASAIAGCLKSEAVVTDAGSVKAAVVEKLEGILG
                     PRYVGAHPMAGSEKSGLDAARKDLFQGAPCIVTPTPRSHAEALAGVEALWREAGCRIF
                     RLSPREHDEAVACVSHLPHAVACCLVETLAARNPDWQKLAGSGYRDTTRVAAGAPEMW
                     NGIFLENRREVQLALREMIAQLQSFSDLLERGDAAALRRLLEKISALRSTLSLSNESV
                     "
     gene            1593..>2111
                     /locus_tag="VIS74_07795"
     CDS             1593..>2111
                     /locus_tag="VIS74_07795"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978216.1"
                     /note="catalyzes the formation of
                     5-O-(1-carboxyvinyl)-3-phosphoshikimate from
                     phosphoenolpyruvate and 3-phosphoshikimate in tryptophan
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEY5793185.1"
                     /translation="MSQYKVKQAPRIETEIRVPGDKSISHRAILLSAISNGTCKITNF
                     LEGEDCRSTMKAMMALGVKIETPEPGTVIVHGNKGVFAQPEESIDCGNSGTTMRLLSG
                     ILAAQPLKVRLTGDESLSRRPMKRVMIPLRQMGARLSAEGAGDTPPLLIEGGSLNGIT
                     YESPVASAQVKSA"
BASE COUNT          392 a          630 c          701 g          388 t
ORIGIN      
        1 gtgtcggtga aaggctggac ggaagagtcg tcgctcaccg cgctcgatct cgcgcgccgc
       61 gccgaggacg ccggcgccgg gacgatcatt tacaccgaca tcgcgacgga tggcatgctg
      121 gaaggcccca actttgccga attggaaaag atgctggggt tgctggattg ccagttgatc
      181 gccagcggcg gggtgtcctc cgccggggat ctgcggagat tggccagact gccggatctc
      241 tatggcgcga tcatcggcaa ggcgttatac gacgggcgga ttaccggtaa tctgcgcgaa
      301 cagatcgttt tggattgacc cggagaagga accgtgaatc ctatccaatt cagggaacta
      361 tgaaatcgaa tttatgcatt ttgggttgcg cgggcctcat ggtagtcatt gccgcctgca
      421 gctcgacggg gtcttccacg gcgcgttccg aaaacaatcg ttacggaaga ggttccaccg
      481 gtggacagcc gacggatacg cagtttatcg aggcgactcc caccccggcc cccagcccca
      541 ctgaaacggc tgcccccgaa ccggcgaccg tcggcgggct gcccacgccg actccggctc
      601 ccgtggtggc acgggatttg ccctacggca ccccggttcc cggcaaaccc ggctttgtga
      661 ccagcccgca cgatcccacg gctggctacg tggatgtgcg cggatttgcg ccggggcagg
      721 aagtgcgctg tccatacacg aacaaaattt tcctcgttcc ctgaagacgg ttgcgatttt
      781 tgcaccgggt ttgatcggag catcattggg gcttgccctg gcggatcgct ggcgggtgcg
      841 cgtctggtcc cgcagtgaat cctccgcgca ggcggccgcc cggcggatcg gcgcggaatt
      901 cgcctccgcg gatccggtgg aaacggcggc gggcgcggat gggattgtct tttgctcgcc
      961 cattgacgcc atgccggcat tggcatcggc cattgccggt tgtttgaaat cggaggcggt
     1021 ggtgacggat gcgggcagcg ttaaagccgc ggtggtcgaa aaactcgaag gcattctggg
     1081 cccccgttac gtcggcgctc atccgatggc cggcagcgag aaaagcgggt tggacgccgc
     1141 gcggaaggac ttgttccaag gggcgccctg cattgtgacg cccacccccc gcagtcatgc
     1201 cgaggcgctg gcgggcgtgg aagcgctctg gcgggaggcc ggctgccgga ttttccgcct
     1261 cagcccgcgg gaacatgatg aagccgtggc ctgcgtgagc catttgccgc acgcggtggc
     1321 ctgctgtctg gtggaaacgc tggcggcccg aaatccggac tggcaaaagc tggccggaag
     1381 cggttaccgt gacaccacgc gcgtcgctgc cggggccccg gaaatgtgga atggcatctt
     1441 tctggagaat cgccgagagg ttcaactggc gcttcgcgaa atgattgccc agctccagtc
     1501 attttccgat ttgctcgaac gaggggatgc cgccgcgttg cggcggctcc tggagaaaat
     1561 cagcgccctg cggagcaccc tttccttatc caatgagtca gtataaagtt aaacaagctc
     1621 cacgcatcga gaccgaaatc cgggttcccg gcgacaagag catttcgcat cgcgccatcc
     1681 tcctcagcgc catttccaat ggcacctgca agatcaccaa cttcctcgaa ggcgaggact
     1741 gccgctccac gatgaaggcc atgatggcgc tgggcgtgaa aatcgagacg ccggaaccgg
     1801 gaacggtcat cgtacacgga aacaaggggg tgtttgccca gccggaggag tctattgatt
     1861 gcggcaattc cggcacgacg atgcggctcc tttcgggaat cctggccgcg cagccgttga
     1921 aggtccggtt gacgggcgac gaatcgctga gccgccgccc gatgaagcgc gtgatgattc
     1981 cgcttcgcca gatgggcgcg cggttgagcg cggaaggagc gggggacacg ccgccgctgc
     2041 tcatcgaggg aggttcgctg aatggcatca cctacgaatc gccggtcgcc agcgcgcagg
     2101 tgaagagcgc g
//
LOCUS       DATDAY010000396         3991 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_856349, whole genome shotgun sequence.
ACCESSION   DATDAY010000396 DATDAY010000000
VERSION     DATDAY010000396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 3991)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3991)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3991
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_856349"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(58..1320)
                     /locus_tag="VIS74_07800"
     CDS             complement(58..1320)
                     /locus_tag="VIS74_07800"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY5793186.1"
                     /translation="MVIIGGGFGGLSAAKVFEGQAVKVILIDRSNHHLFQPLLYQVAT
                     AGLAPADIAQPIRSILRDQENASVVLDEVTAIKTATREVVTPDITFKYDYLIVAAGAR
                     HSYFGHDEWEPFAPGMKSLEDAVEIRRRILVGFEVAEKALSDEERKAALTFVVVGAGP
                     TGVEMAGAIAELARFTLKKDFRRINPADAHVMLVEAGPRVLPPFSPVSSERTVAQLKA
                     LGVDVRTGCAVTQIAEGEAVIGGEHIKTNTIIWAAGNKASPLAGMLGAELDRQGRVTV
                     NPDLSVNGHPEVFAVGDMAFATFKGGKPVPGVSPAALQGGRHAAQNILRLIKGEKTQK
                     WEYFDKGSMATIGRHSAVAEVGPLKLSGISAWLGWAFVHLFFLIGFKSRIMVFFEWAW
                     AYFTYSKGSRLISTAPGKWGVLRDPKKS"
     gene            1430..2677
                     /gene="nuoD"
                     /locus_tag="VIS74_07805"
     CDS             1430..2677
                     /gene="nuoD"
                     /locus_tag="VIS74_07805"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018052518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase (quinone) subunit D"
                     /protein_id="HEY5793187.1"
                     /translation="MATIYEQADPLARSVEQLRAAQEAGEMLGETLTLNMGPSHPSTH
                     GVLRILLELDGELITKADPDVGYLHRGDEKIAENMQYNQFVPYTDRLDYLAPLANNVA
                     YALAVEKLMGWELPPRGKAIRVICCELARISAHLLGIGAFAMDLGAITVFLFTFTERE
                     KIYNLIEQLTGARFTTSYTRVGGQTRDLPETFKAQLLEFIRTFRQSADEVDRLLTRNR
                     IFVDRTKDVGVISRADAIAYALSGPNLRGSGVEHDLRRKHPYLDYEKYDFEVPVGSAG
                     DCYDRYLVRFEEMRQSTRILEQAINDLPGGPINYFDPKSSLPPKVEVLTKMEELIHHF
                     LVATQGVEAPPGEVYFGAENPKGELGFYICSKGGGVPYRLKIRAPSFVNLSILPKILP
                     GHMIRDVVAILGSVDFVMGESDR"
     gene            complement(2804..>3991)
                     /gene="lpxK"
                     /locus_tag="VIS74_07810"
     CDS             complement(2804..>3991)
                     /gene="lpxK"
                     /locus_tag="VIS74_07810"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HEY5793188.1"
                     /translation="TMKRWLEALEAFAIDVILERRYGKRASLLRGVLYFLSLIYQNIV
                     QLRLRLFRNRLLRAQSLGCPVISVGNLTVGGTGKTPVVEMLARELTRQGRRVGILTRG
                     YKSVPKPLLRRLGDKLFKNKAVFAPRIVSDGRTLLLDSLTAGDEPFMLANNLRGVVVL
                     VDRDRVKSGYYAIEHFGCDTLLLDDGFQYLRLRHSYEIALVDRQAPFGNRFILPRGTL
                     REPKENLCRATHIFVTKSSAEPDTPLLEEIRQYNRTADIIECAHRPVHFRDLISGEIH
                     PLDFVKGKRAASLCGIAVPESFEEALRKLGAGLELTEAFTDHHRFTAREIESFLRRCG
                     RRGVEMILTTEKDAVRIPRILDAEVPIYYLRVEIEILRGQEQWEALVRRLAPAAAPEF
                     AAV"
BASE COUNT          737 a         1234 c         1286 g          734 t
ORIGIN      
        1 aaccgggagc gcgcggcccg cggcgggcgg cgccggcgct cccggctttt tcatgggtca
       61 ggattttttg ggatcccgca gcacgcccca ttttccgggg gcggtggaga tcaggcgcga
      121 tcccttggag taggtgaaat aggcccaggc ccattcgaag aagaccatga tgcggctctt
      181 gaatccgatg aggaagaaga ggtgcacgaa ggcccagccg agccaggcgg agatgccgct
      241 gagtttcagc gggccgactt cggcaacggc ggagtggcgg ccgatagtcg ccatgctgcc
      301 cttgtcgaaa tactcccatt tctgggtctt ttcgcccttg atcaaccgca ggatgttctg
      361 cgcggcgtgc cgtccgccct gcagggcggc ggggctgacg ccgggaaccg gcttgccgcc
      421 cttgaaggtg gcaaaggcca tgtcgcctac ggcgaagact tccggatggc cgttgacgga
      481 gaggtccggg ttcaccgtaa cgcggccctg gcggtcgagt tcggcgccga gcatgccggc
      541 gagcggggag gccttgttgc cggcggccca gatgatggtg ttcgtcttga tgtgttcacc
      601 gccgatgacg gcttcgcctt cggcgatctg ggtgacggcg cagccggtgc ggacgtccac
      661 gccgagggcc ttgagctgcg cgacggtgcg ttccgaggaa accggcgaga agggcggcag
      721 cacgcggggg cccgcttcca cgagcatcac gtgggcgtcg gcgggattga tgcggcggaa
      781 gtccttcttc agcgtgaagc gggcgagttc ggcgatcgct cccgccattt ccacgccagt
      841 cgggccggcg cccacaacca cgaaggtcag ggccgccttg cgctcctcgt cgctgagggc
      901 tttttcggcg acctcgaagc cgaccaaaat ccggcggcgg atctccacgg catcttccag
      961 gctcttcatg cccggggcga agggttccca ttcgtcgtgg ccaaagtagc tgtggcgcgc
     1021 gccggcggcg acgatgaggt aatcgtattt aaaagtgatg tccggcgtga cgacttcgcg
     1081 ggtggcggtc ttgatggccg tgacctcgtc gaggacgacg ctggcatttt cctgatcgcg
     1141 caagatgctg cggatcggct gggcgatgtc cgcgggggcc aggccggccg tggcaacttg
     1201 atacaacaag ggttggaaga gatggtgatt actgcggtcg atcaggatga ctttgaccgc
     1261 ctggccctcg aagaccttgg cggcgctcag ccccccgaat ccaccaccaa taatcaccac
     1321 aacggggagg cttgattttt caggcgcttg ggatgattct gacatggttc ccagctttgt
     1381 gaaaaatttc tcaaggcaac acaaggggaa tttgaaaggc cgacccccaa tggcaacgat
     1441 atacgaacaa gcggaccccc tggcccgcag cgtggaacag ttgagagccg cccaggaagc
     1501 gggcgaaatg ctgggtgaaa ccctcaccct gaacatgggg ccgtcccatc cctccacgca
     1561 cggcgtgtta cgaattcttc tcgaactcga cggggaattg atcaccaagg ccgatccgga
     1621 tgtgggctac ctgcaccgcg gggatgaaaa aatcgcggag aacatgcaat acaatcagtt
     1681 cgtgccctac acggaccggc tggattacct ggcgccgctg gccaataacg tggcctatgc
     1741 cctggcggtg gaaaaactca tgggctggga gctgccgccg cggggcaagg cgatccgcgt
     1801 gatctgctgc gagctggccc gaatctccgc gcacctgctg ggaataggcg ccttcgccat
     1861 ggacctgggg gcgatcaccg tcttcctctt caccttcacc gagcgggaga aaatttataa
     1921 tctcatcgag cagctcaccg gcgcccgctt cacgacgagc tacacgcggg tcggcggcca
     1981 gacgcgcgac ctgccggaga cgttcaaggc gcagttgctg gaatttatcc gcaccttccg
     2041 ccaaagcgcg gacgaggtgg accggctcct gacgcggaat cgcatcttcg tggatcgcac
     2101 gaaggatgtc ggcgtgattt cccgggcgga tgccatcgcc tacgccctgt ccggcccgaa
     2161 cctgcgcggc tccggcgtcg aacacgatct gcgccggaag catccctacc tcgattacga
     2221 aaaatacgac ttcgaggttc ccgtcgggag cgcgggggat tgttacgatc gttacctcgt
     2281 ccggttcgag gagatgcgtc aaagcacgcg catcctggag caggcgatca acgacctgcc
     2341 cggcggcccg atcaattatt tcgatcccaa gagcagcctg ccgccgaagg ttgaagtcct
     2401 caccaaaatg gaggagctga tccatcattt cctcgtcgcc acgcaggggg tggaggcgcc
     2461 gccgggggag gtttatttcg gcgcggaaaa tcccaagggc gagctcggct tctacatttg
     2521 cagcaagggc ggcggcgtgc cgtatcgcct taagattcgc gcgccctcat tcgtgaatct
     2581 gagcatcctc ccgaaaatcc tgcccgggca catgatccgc gatgtcgtgg cgatcctcgg
     2641 cagcgtggac tttgtcatgg gcgaaagcga ccggtaggtt ttttattcac cacagaggac
     2701 accgagagca cggaggtgaa gaccgggaaa aatttcccga aaatttcggt tttatcctct
     2761 tctccgtgct ctctgtgtcc tccgtggtaa aatcctcaag caatcagact gcggcaaatt
     2821 caggcgccgc ggccggcgcc agccgccgga ccagcgcctc ccattgttcc tggccgcgca
     2881 ggatttcgat ctccacgcgc aggtaataaa tcggcacctc cgcatcgaga atgcgcggga
     2941 ttcgcacggc gtccttttcc gtggtcagga tcatctccac gccgcgccgc ccgcagcgcc
     3001 gcaggaagct ctcgatctcc cttgcggtga agcggtggtg gtcggtgaac gcctccgtca
     3061 gctccaggcc ggcgcccagc ttgcgcagcg cctcctcgaa gctctccggc acggcgatgc
     3121 cgcagaggga ggccgcccgt tttcccttca cgaaatccag cggatgaatc tcgccgctga
     3181 tcaaatcccg aaaatgcacg ggccggtggg cgcattcgat gatgtccgcc gtgcggttgt
     3241 attgccggat ctcctcgagc agcggggtgt ccggctccgc cgaggatttg gtgacgaaaa
     3301 tgtgcgtggc gcggcaaagg ttttccttcg gctcgcgcag ggtgccgcgg gggaggatga
     3361 agcgatttcc aaagggcgcc tgccggtcca cgagcgcgat ctcgtaggaa tgccgcagcc
     3421 gcagatactg gaagccgtcg tcgagcagca gggtgtcgca gccgaaatgt tcgattgcgt
     3481 aatacccgct cttcacccgg tcgcgatcca ccagcacgac cacgccgcgc aggttgttcg
     3541 cgagcatgaa gggctcgtct cccgccgtga gcgaatccag cagcagcgtg cgcccgtcgg
     3601 aaacgatgcg cggggcgaag accgccttgt ttttaaagag cttgtcgccc agccgccgca
     3661 gcagcggctt tggcacgctt ttgtagccgc gcgtcaggat gcccacgcgc cgcccctgcc
     3721 gggtgagttc gcgcgccagc atttcgacga ccggcgtctt gcccgtgccg ccgacggtga
     3781 gattgcccac gctgatgacc gggcagccca gcgattgcgc gcgcagcagg cggttccgaa
     3841 acagccgcag ccgcagctgc acgatgttct ggtaaatcag cgacaggaaa tagagcacgc
     3901 cccgcagcag gctggcccgc ttgccgtagc ggcgctccag aatgacgtcg atggcgaacg
     3961 cctccagcgc ttccagccag cgtttcatgg t
//
LOCUS       DATDAY010000397         4540 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_138028, whole genome shotgun sequence.
ACCESSION   DATDAY010000397 DATDAY010000000
VERSION     DATDAY010000397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 4540)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4540)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4540
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_138028"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..524)
                     /locus_tag="VIS74_07815"
     CDS             complement(<1..524)
                     /locus_tag="VIS74_07815"
                     /inference="COORDINATES: protein motif:HMM:NF020419.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HEY5793189.1"
                     /translation="MNEAPRRYATAAAFRVALETRLKAISTAEDTDLQRLRRQVSFDR
                     LLARFFAEQNAPWLLKGGYAMELRLHAARTTKDIDISLPSESVTGFGGNILAGLQDQA
                     GADLADFFTFTIADPQMDLKAAPEGGARYPVTASLAGRTFTKFHLDVGIGDAVTPPTE
                     LIEARDWLGFAGIA"
     gene            complement(521..1126)
                     /locus_tag="VIS74_07820"
     CDS             complement(521..1126)
                     /locus_tag="VIS74_07820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5793190.1"
                     /translation="MKRSPKESQANLFAIAEGQGGFFTAKQADEAGFDRTHHAYHVGV
                     GNWQREHRGIFRLAQYPMPERPDLILWSLWSRNREGVPEGVYSHQTALSLYDLTDLMP
                     SKLHMTVPTKFRRMADPPAILELHFADLPSSEIEEREGYQVTRPIRAVVDVSRDVAPE
                     ILIEAFSEGRTRGLITEKDVTQYREQLPAFLFPNSRELQTA"
     gene            complement(1194..2486)
                     /locus_tag="VIS74_07825"
     CDS             complement(1194..2486)
                     /locus_tag="VIS74_07825"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEY5793191.1"
                     /translation="MATIIPKNSKSERGPLEIIEGKGIKIPIYLALRRGEESYLLAYY
                     KEGLRKMERVASIEDARRRAKVLIDELSAGKVHVTTFTLKQTTAITDAVEIISPFKLS
                     ITEVARQFAEAQKLLGEVPILHAAQFYAKHLEGEAKHGALETITLPDLVTRYLASIEG
                     KKSKRYVEDLKAKLKRAAKAFTGQIREIRATDLDRWIDGMEDAGLRTKNNYRMALITL
                     FSYARDKNFLPRGQQTEAEFVTRYNDKKGGQIGIYSPQQFATLLRYVDGRFLPFVALG
                     GFAGLRSIEILRLEWTDVWFEKGFIEVGRHKSKTATRRLVPICPALAAWLKPHAKKSG
                     PVLPDIRNEVHFTRLFHAAKSTLNDAEGKPRVELVHNGLRHSFCSYRMAETKSAPQVA
                     LEAGNSPKMLFEHYRELVTEAEAKEWFALAPVKVKRPK"
     gene            complement(2623..3099)
                     /gene="cadR"
                     /locus_tag="VIS74_07830"
     CDS             complement(2623..3099)
                     /gene="cadR"
                     /locus_tag="VIS74_07830"
                     /inference="COORDINATES: protein motif:HMM:TIGR02047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cd(II)/Pb(II)-responsive transcriptional
                     regulator"
                     /protein_id="HEY5793192.1"
                     /translation="MKRLQGQWLTTQKQAMKIGELAATVQCTVETIRYYEKEGLLPKP
                     ARGGNNYRFYGPNHRQRLLFIRNCRALDMSQEEIRALLGLKEKSEGDCGSVNALLDKH
                     ISHVDARIGELRRLKSQLTGLRQKCRNKTTVSDCRILQGLSEIRPGDRPPRATHLG"
     gene            3121..>4540
                     /locus_tag="VIS74_07835"
     CDS             3121..>4540
                     /locus_tag="VIS74_07835"
                     /inference="COORDINATES: protein motif:HMM:TIGR01525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEY5793193.1"
                     /translation="MSCSCKHHDEEDSPSGTVPAGASEETFRVSGMDCSEEVAAIERA
                     LKPVGGVLGVRANLVTSTVTVYHDGSVAKAGLIRAIHQSGVKVQAGEGAPGGSPISLA
                     AWLVGISGFSTGLGIVLEWFGLRGTLWPGAAFTLAILAGGALVFPKALRSLKTFSLDM
                     NVLMTVAVIGAAAIGQPAEGAAVVFLFSLSELLESWSVGRARCAIQALLQLAPDTALV
                     KRRGKIQEVPAAEVTAGETILVKSGQKVPLDGAVLAGSSAIDQAPITGESMPVEKAPG
                     DPVYAGTINGEGSLEIQTTKAAGDTTLARIIKLVEEAQRQKAPAQRFVDGFAKYYTPA
                     VMILALFAGLVPPLFFGAAWMDWIYRALVLLVIACPCALVISTPVSIVSGLTAMARRG
                     VLIKGGAYLEALGGLRVLAMDKTGTLTEGKPRVLDVRPFDSLPAEDILRIAAAIDTHS
                     THPLARAVVRRAEESQIAFDPAG"
BASE COUNT          870 a         1346 c         1305 g         1019 t
ORIGIN      
        1 ctggcgattc cggcgaatcc cagccagtca cgagcctcga ttagttccgt cggaggggtc
       61 acggcatctc caattccgac atcgagatga aatttcgtga acgtccggcc cgcaagggaa
      121 gccgtcacag gataccgtgc gcccccctcc ggcgcagctt tcaagtccat ttgtgggtca
      181 gctatggtaa atgtgaagaa gtcagccaag tccgcccccg cttgatcctg tagtcctgcc
      241 agaatgtttc cgccaaaccc cgtcactgac tcggagggta gggaaatgtc gatgtctttc
      301 gtcgttcgtg cggcgtggag tcgcaattcc atcgcgtagc cacctttaag gagccaaggc
      361 gcattctgtt ccgcgaagaa gcgggccaag agccggtcga atgaaacctg ccgtcgaagg
      421 cgttgcaagt cggtgtcttc ggctgtcgaa atcgctttga gccgtgtttc gagtgctacg
      481 cggaatgccg cagcggttgc atatcggcgg ggagcttcgt tcatgcggtt tgaagttctc
      541 ggctgttagg aaaaagaaaa gcggggagct gttcccggta ttgcgtcaca tccttttcgg
      601 tgatgagtcc tcgggttctt ccttctgaaa atgcctcgat gaggatttct ggagccacat
      661 cacggctgac atccacgaca gcccgaatcg ggcgtgtgac ctgatacccc tcgcgctcct
      721 caatttcgct cgaagggaga tctgcaaaat gtagctccag aatggccgga gggtctgcca
      781 tgcgtcggaa ttttgtcggc accgtcatgt gaagtttcga cggcatcaga tcggtgaggt
      841 cgtagagact cagcgccgtt tgatgcgaat aaacgccttc tggcacaccc tcacgattcc
      901 gggaccagag ggaccaaagg atgaggtccg gacgctctgg cattggatat tgcgccagcc
      961 ggaatatgcc ccggtgctcc cgttgccagt ttcccacccc gacgtggtag gcgtgatgtg
     1021 tccggtcgaa tccggcttcg tccgcttgct tggcggtaaa aaaccctcct tgaccttccg
     1081 caatggcgaa aaggtttgct tgggattctt tgggtgatcg tttcacgaga attcagccta
     1141 ccatttgaag gctgggcagc aacataaata gcgcatttat tgcgctgttt atgctattta
     1201 ggccgtttca cttttaccgg tgcgagagca aaccattcct tggcttccgc ctccgtcacc
     1261 agctcccggt aatgctcgaa cagcattttt gggctatttc cggcctccaa ggcgacctgt
     1321 ggtgcgcttt tcgtttcagc catgcggtag gagcagaagg aatggcgcag accgttgtga
     1381 acgagctcca cacggggttt tccctccgcg tcgttgagtg tggatttcgc cgcgtggaaa
     1441 aggcgcgtaa aatgaacctc gttgcgaatg tcgggaagca ctggcccgga ctttttcgcg
     1501 tggggcttca gccacgccgc caatgccgga cagatcggca caagccgccg agtggcagtc
     1561 tttgatttgt gtcggcccac ttcaatgaag cctttttcaa accaaacatc cgtccactcc
     1621 aagcgtagga tttcaatgct gcgcaaaccg gcaaatccgc ccagcgcgac gaagggcaga
     1681 aaccttccat cgacataccg gagcagcgtg gcgaactgct gcgggctgta aatgccaatc
     1741 tgcccgcctt ttttgtcatt ataccgtgtg acgaattccg cctcggtctg ctgcccacga
     1801 ggcaggaagt ttttgtcgcg ggcataggaa aacaatgtaa tcagggccat ccgataattg
     1861 ttttttgtgc gcaagcctgc gtcctccatg ccgtcgatcc atctgtccaa atcggtggcc
     1921 cggatctccc gaatctggcc ggtgaaggcc ttggccgccc gtttcagctt ggctttcaaa
     1981 tcttccacgt agcgctttga tttcttcccc tcaatggacg caaggtatct cgtaactagg
     2041 tcggggagtg tgatcgtctc caacgcgccg tgcttcgctt ctccttcaag gtgtttggca
     2101 taaaactgcg ccgcatgcag aatggggact tcgcccaaaa gtttttgggc ttccgcgaat
     2161 tggcgagcga cttccgtgat gctgagcttg aacggactga tgatttccac agcatcagtg
     2221 atggcagtcg tctgcttgag cgtgaacgtg gtgacatgca ccttgcctgc cgaaagttca
     2281 tcaatgagaa cttttgcccg acgccgggca tcctcgatgc tggctacccg ttccatcttt
     2341 cgcagccctt ccttgtagta ggcgaggaga taactttctt ccccacgccg cagcgcgagg
     2401 tagattggaa ttttgatgcc cttaccttcg atgatttcaa gcgggccgcg ctcgctcttg
     2461 ctgttcttgg ggatgattgt tgccatcgga taaccttgat tgttacccaa gtgttaccca
     2521 aagcctaaaa agagtcaaat acggagtaaa atcaacgtat tcgaaccctg taccgcgcac
     2581 cagtttccgg aacgcagatg caaatgcggc ccacccgccg agtcatccaa gatgcgtggc
     2641 gcggggcggc cggtctccag gccggatttc tgaaaggccc tgcagaattc gacaatcact
     2701 taccgtggtt ttgttcctgc atttttgccg cagcccggtg agttggcttt tcaagcgccg
     2761 caattcccca atgcgcgcat ccacatggga gatatgttta tccagcaagg cattgacgga
     2821 accacaatcg ccttccgatt tctccttgag cccaagcaag gcgcgaatct cctcctgaga
     2881 catatccagc gcgcggcagt tgcggataaa taacaggcgc tgccggtggt ttgggccgta
     2941 aaagcgatag ttgttcccgc cccgcgccgg tttcggcaaa agcccctcct tctcgtaata
     3001 ccggatggtt tccaccgtgc actgcaccgt cgccgcgagt tctccaatct tcatcgcttg
     3061 tttttgtgtc gtgagccatt gaccctgaag ccgcttcaag gtgtttactg aaaatacgcc
     3121 atgtcttgct catgtaaaca tcacgacgaa gaggattcac cgtccggcac ggttcccgcc
     3181 ggggccagtg aggaaacctt tcgcgtgagc ggcatggatt gctccgagga agtggcggcc
     3241 atcgagcggg cattaaagcc ggtcggcgga gtcctggggg tccgggcgaa tctcgtcacc
     3301 tcaactgtca ccgtctatca cgacggatcg gtggcgaaag ccgggttgat ccgggcgatt
     3361 caccaaagcg gggtcaaggt gcaggcgggc gagggggctc cgggcggaag cccgatctcg
     3421 ctggcggcgt ggctggtcgg aatatccgga ttttccaccg gcctcggaat cgtcctggaa
     3481 tggtttggcc tgagagggac tctctggccc ggcgcggcat ttaccctcgc catcctcgcc
     3541 ggcggcgccc tggtttttcc aaaggcgctg cgatccctga agaccttctc cctggatatg
     3601 aacgtgctga tgaccgtggc cgtcatcggc gcggccgcca tcggccagcc cgcggaaggc
     3661 gcggcggtgg tgtttctctt ctcgctttcc gaactgctgg agtcgtggag cgtgggccgg
     3721 gcccgctgcg ccattcaagc cctcctgcag cttgcgcccg atacggccct ggtgaaacgg
     3781 aggggaaaaa ttcaggaggt tcccgccgcg gaagtcaccg ccggcgaaac catcctcgtc
     3841 aaatccggcc agaaagttcc cctcgatggc gccgtcctcg cgggatcatc cgccattgac
     3901 caggcgccga tcaccggcga atccatgccc gtcgagaaag cgccgggcga tcccgtgtat
     3961 gccggcacga tcaacggcga aggctcgctc gaaattcaaa ccaccaaggc ggcgggcgat
     4021 accacgctcg cgcgcatcat caaactcgtc gaggaggccc agcggcaaaa ggccccggcc
     4081 cagcggttcg tcgatgggtt tgccaaatac tacacgcccg ccgtgatgat cctcgcgctg
     4141 ttcgccggcc tcgtgccgcc gttgtttttc ggcgcggcgt ggatggattg gatttaccgc
     4201 gcgctcgtgc ttctggtcat cgcctgcccg tgcgcgctcg ttatttccac gcccgtcagc
     4261 atcgtttccg gcctcaccgc gatggctcgc cgcggcgtcc tcatcaaggg cggcgcgtat
     4321 ctggaggcat tgggcggctt gcgcgttctg gcgatggaca aaaccggcac cctcaccgaa
     4381 ggcaaacccc gcgtgctcga cgtgcggccg ttcgacagcc tgccggcgga ggacatcctt
     4441 cggatcgccg ccgcgattga cacccattcc acgcatccgc tggcccgggc ggtggttcgc
     4501 cgcgccgaag aatcccaaat cgccttcgat cccgccggga
//
LOCUS       DATDAY010000398         2083 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_2108544, whole genome shotgun sequence.
ACCESSION   DATDAY010000398 DATDAY010000000
VERSION     DATDAY010000398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2083
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2108544"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..306)
                     /locus_tag="VIS74_07840"
     CDS             complement(<1..306)
                     /locus_tag="VIS74_07840"
                     /inference="COORDINATES: protein motif:HMM:NF014460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY5793194.1"
                     /translation="MRRIVRAVYEQGALCLPVILIVGMFTGLVLGLQGYYVLNRFGSA
                     GLLGALVSLSLVREMAPVLAALMLVGQAGSALAAELGIQRNSEQIVALETMGESSHGY
                     "
     gene            626..841
                     /locus_tag="VIS74_07845"
     CDS             626..841
                     /locus_tag="VIS74_07845"
                     /inference="COORDINATES: protein motif:HMM:NF021474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HEY5793195.1"
                     /translation="MRITIEIEDKKLKSILKWTGQRKKSPAVASALEEYLEQKKRQAF
                     VTKVMEGGTDYRASNDEVEAQASLDRP"
     gene            838..1245
                     /locus_tag="VIS74_07850"
     CDS             838..1245
                     /locus_tag="VIS74_07850"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HEY5793196.1"
                     /translation="MILIDTSAWIEFFRARGEPALKSRVADCLSIGQAAYTCPIRFEL
                     LLGARPSEVSDLLIGLDLARRLALTASHWDAAATLGSRLRADGVTVPASDLLIATLAE
                     KEGIPLLARDRHFEIIRDSVLPKMKLVVSGDRL"
     gene            1310..>2083
                     /locus_tag="VIS74_07855"
     CDS             1310..>2083
                     /locus_tag="VIS74_07855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5793197.1"
                     /translation="MNIPRPAPPPLRPVLLCAMAACLVLHASAQPPPMSAMAIARETS
                     DPTSDLWYLFTELAFATQPGEPFDRTNQFTLELQPTMPVPLTDSWRLLNYPDLILASE
                     GTPDGLQITGVKSFSWTAALSPVTGRLGWAYGLGPHVSFPVATTGAFGADPWQFGVGG
                     VLAWRSETFLASAQVNSGWTTSGQSREAGSFEIQYNLQHFFRNGTQIGLGHPRVEYHW
                     DRDGRGGWDVPVGVDVAKVFHIGRLPVKVMLEYDFYVLSD"
BASE COUNT          387 a          693 c          641 g          362 t
ORIGIN      
        1 gtagccatgg ctgctctcgc ccatcgtctc cagcgccacg atctgctccg agttgcgctg
       61 gatgccgagt tcggccgcca gggcggaccc cgcctgcccc accagcatca gcgccgccag
      121 caccggcgcc atttcccgca ccaggctgag cgaaacgagc gcccccagca aacccgccga
      181 gccaaagcga ttcaggacgt aataaccctg caatcccagc accaggccgg taaacattcc
      241 cacgatcagg atcaccggca ggcacagcgc gccctgctca tacacggcgc ggacgatccg
      301 gcgcaccagc ctccgcgacc cgaccgcgga acgaaacgac ttcccggtac atagagcgaa
      361 atcgcccagt ccggaaacaa tgtgaaggct ctgcgcgcca agcaatcgca acatcccggg
      421 gattgatcgc aggaagaaaa aggttgtgca atgccacggt tgcgggagtc cgacttcgag
      481 gaatctcccg cggaggcgcg gagacgcgga ggggaaaggc tttaaacgcg gaaaagggag
      541 aaaaaggaaa aataagctct gcggaatcgc gcttgccgct tcgggcgccc ctggctagat
      601 ttgatgcaaa tccactttgg aaaagatgag aattaccatc gagattgagg ataagaagct
      661 gaagtcgatt ctcaagtgga ccggccagcg gaagaaatcc cccgcggtcg ccagcgcgct
      721 cgaggagtat ctggagcaga agaagcgcca ggcttttgtg acgaaagtca tggagggcgg
      781 gaccgattat cgcgcgtcca atgacgaggt ggaggcgcag gccagcctgg atcggccgtg
      841 atcctcatcg acacgtcggc gtggatcgag tttttccgtg ctcgcggcga gccggccttg
      901 aagtcccgcg tggcggattg cctttccatc gggcaggcgg cctacacctg cccgatccgt
      961 ttcgagttgc tgctgggcgc gcgcccttcc gaagtcagcg acttgctcat cggcctggac
     1021 ctcgcccggc gcctggctct caccgcgagt cattgggatg ccgcggcgac gctcggcagt
     1081 cggctccgcg cggatggcgt caccgttccg gcttccgatc tcctgattgc cacactggcg
     1141 gagaaagaag gcattccgct gctcgcccgc gaccgccatt ttgaaatcat ccgcgattcc
     1201 gttttgccga agatgaagct tgtcgtttca ggcgaccggc tctgagcgtt ttcgtcccac
     1261 ttatccgatt ggaaaccccg tcctgggcat ccatgctccc gcgcatcgca tgaacatccc
     1321 gcgtccggcg ccgcccccgc ttcgccccgt cctgctttgc gcgatggccg cgtgcctcgt
     1381 tctccacgcg agcgcgcagc cgccgccgat gtcggccatg gcaatcgccc gggagacgag
     1441 cgacccgacg tccgacctgt ggtatctctt caccgaactc gcgttcgcca cgcagccggg
     1501 cgagccgttc gaccgcacga atcaattcac gctcgagctc cagcccacga tgcccgtgcc
     1561 gctcacggat tcctggcggc tgttgaacta ccccgacctc atcctggcca gcgagggcac
     1621 gccggatggc ctccagatca ccggagtgaa gagcttttcg tggaccgccg cgttgagccc
     1681 ggtgaccggg cgtctcggct gggcgtatgg actcggcccg catgtctcgt ttcccgtcgc
     1741 cacgacgggg gcgttcggcg ccgatccgtg gcaattcggc gtcggcggcg tgctcgcatg
     1801 gcggtccgaa acgttccttg cctccgcgca ggtcaactcc ggctggacga cctccggcca
     1861 aagccgcgag gccggttcgt tcgagatcca atacaacctc cagcattttt tccgcaacgg
     1921 cacgcagatc ggcctcggcc acccgcgggt ggaataccac tgggatcgtg acggccgggg
     1981 cggctgggac gtgccggtcg gcgtggacgt ggcgaaggtc ttccacatcg gccggctgcc
     2041 ggtgaaagtg atgctggaat acgacttcta cgtgctgagc gac
//
LOCUS       DATDAY010000399         2003 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_1915222, whole genome shotgun sequence.
ACCESSION   DATDAY010000399 DATDAY010000000
VERSION     DATDAY010000399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2003)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1915222"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1022)
                     /locus_tag="VIS74_07860"
     CDS             complement(<1..1022)
                     /locus_tag="VIS74_07860"
                     /inference="COORDINATES: protein motif:HMM:NF012686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5793198.1"
                     /translation="MNSEALSGLWLDGAVVFVYFALILGIGLYVGRGSKNLESFAVGN
                     RNIPWWAVLASVLAAEISAATFLGAPGEGYEKRNFTYAQLAIGTILARVIIAFVFIKP
                     YYDFRVVSIYEYLKLRFGTRTKNAASAIFLVTRALASGTRLYVAAIVFVLGYEMITHQ
                     TLGIWQQVWVYVGALTFITLLTAIYTALGGIKAVVWTDVIQAVIMFGSLGFAIWSLLG
                     HIPGGWHGVAAAMNQPGDWQFFSTGISEDRSFWGNVRGILESEYTIWAALIASTFTTM
                     ATHGTDQDMVQRLLTAKDHVRSRISLILSGLADLPLVMMFLLVGILLWVFYQQPDKNA
                     PFAYYIV"
     gene            complement(1019..>2003)
                     /gene="argA"
                     /locus_tag="VIS74_07865"
     CDS             complement(1019..>2003)
                     /gene="argA"
                     /locus_tag="VIS74_07865"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HEY5793199.1"
                     /translation="IMEGLSAVDLRAAYANAIISHPAGIFGGVDYEYSGRVERVDFKA
                     LNLLLEEGIVPVIPPMGFDGEGRTFRVNSDGVAVEVAEALRAAKLIYLAPPTISEIAQ
                     ELPRQLSISETEEFLKKRGSAHQPGMVSKLECAARACRQGVPRVHLLDGSVNEALLAE
                     VFSNEGIGTMVYSNEYQQIRRIFKKDVRGVLSLIRQSVKSEELVRRTRTEILEHIEDY
                     WILEIDRNPTACVALHVYPEEKSGELACLYVSKNHENQGYGRKLMKFVENLAKEKGMN
                     QLFALSTQAAHYLQQKGGYKEARPESLPAARREKYEASGRNSKILVKALHG"
BASE COUNT          400 a          662 c          561 g          380 t
ORIGIN      
        1 acgatgtaat aggcaaaggg cgcattcttg tccggctgct ggtaaaagac ccataagagg
       61 attcccacca gcaggaacat catcaccaac ggcaaatccg ccaggccgga caggatcagc
      121 gagatgcggc tgcgaacgtg gtccttcgcc gtcagcaggc gctggaccat gtcctggtcc
      181 gtgccatgcg tcgccatggt cgtgaaggtg gaggcgatca acgccgccca gatcgtgtat
      241 tcgctttcca ggatgccccg gacatttccc cagaaactcc ggtcctccga gattccggtc
      301 gaaaaaaact gccagtcgcc gggctgattc atggccgcgg ccactccgtg ccatcccccg
      361 gggatgtgcc cgagcaggga ccagatcgca aatcccaggg agccgaacat gatcaccgcc
      421 tgaatgacgt ccgtccacac gaccgccttg atgccgccca gcgcggtgta aatcgcggtc
      481 agcagcgtga tgaacgtcag cgcgccgaca tacacccaaa cctgctgcca gatgccgagc
      541 gtctgatggg tgatcatttc atacccaagg acaaacacga tggccgccac gtaaagccgc
      601 gtgccgctgg ccagcgcgcg ggtgaccagg aaaatggccg acgccgcgtt cttcgtgcgc
      661 gttccgaacc gcagcttcag gtattcgtaa atcgaaacca cccggaagtc gtagtagggc
      721 ttgatgaaga caaaggcgat gatgacccgc gcgaggatcg tgccgatcgc cagctgggca
      781 tacgtgaagt tccgtttttc gtagccctcg cccggcgcgc cgaggaaggt ggcggcgctg
      841 atttcggccg ccagcaccga ggccaggacc gcccaccagg gaatgttgcg attccccacg
      901 gcaaagctct ccagattctt gctgccccgg ccgacgtaga ggccgatccc caggatcagc
      961 gcgaaataaa caaaaacaac cgccccatcc agccacagtc ccgataaggc ctccgagttc
     1021 atccgtgcag cgccttgacc aggattttgg aattccgccc gctcgcctcg tatttttccc
     1081 ggcgcgccgc cggcaggctt tccggccggg cctccttgta gccgcctttt tgctgaaggt
     1141 aatgggccgc ctgcgtggaa agcgcaaaca gctgattcat ccctttttcc ttcgccagat
     1201 tttccacgaa tttcatcagc ttgcgcccgt agccctggtt ctcgtggttc ttgctcacgt
     1261 agaggcacgc cagctcgccc gatttttcct cgggatacac gtgcagcgcc acgcaggcgg
     1321 tcggatttcg gtcaatttcc agaatccaat aatcctcgat gtgctcgagg atctccgtcc
     1381 gcgtcctccg gacgagttcc tcgcttttga cggactgccg gatgagcgaa agcaccccgc
     1441 gcacgtcttt tttgaagatt cgccggattt gctggtattc gttggaatac accatcgtgc
     1501 cgatgccctc gttgctgaag acctcggcga gcagggcctc gttgacactg ccatccagca
     1561 aatgcacccg cggcacgccc tggcggcagg cccgcgcggc gcattcgagt ttggaaacca
     1621 tccccggctg atgcgcggag ccgcgttttt tcaaaaattc ctcggtttcg gagatcgaaa
     1681 gctgccgggg caattcctgc gcgatttccg aaatggtcgg cggcgccagg taaatgagtt
     1741 tcgccgcccg cagcgcctcg gccacctcca cggccacccc gtccgaattc acccggaacg
     1801 tccggccctc gccgtcgaaa cccatcggcg gaatcaccgg cacgatcccc tcttccagca
     1861 gcaggttgag cgccttgaag tccacccgct ccacccggcc gctgtattcg taatccacgc
     1921 cgccgaaaat gccggcggga tgggaaatga tcgcattcgc atacgcggcc cgcagatcca
     1981 cggcggagag cccctccatg atc
//
LOCUS       DATDAY010000400         7311 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_673670, whole genome shotgun sequence.
ACCESSION   DATDAY010000400 DATDAY010000000
VERSION     DATDAY010000400.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 7311)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7311)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7311
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_673670"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            96..1004
                     /locus_tag="VIS74_07870"
     CDS             96..1004
                     /locus_tag="VIS74_07870"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY5793200.1"
                     /translation="MSVWVVINNYNYGRFLADALQSVLAQTRFPDRICVVDDGSTDDS
                     RQLLRKEFKDRVEVVEKPNGGQLSCFNEIAGRVGENDVIFFLDADDRFRPGHLEKCLA
                     VYEQHPQVDFVFTRHQRFGEESGPGVILCDPLKGDLGFSVFSARNELVWRGGPTSTLS
                     LRGRILRRFLPCPLENDWRVRADDVLVFGSSLAGARKYFLDQPTVDYRVHASNHFHNR
                     SSALEDSFAYVLRRERLLAWLETHLGMPFRREAILIAIEYKCLPGGKAAEFWRYLKMV
                     AVCRSPLPEKGRALLSLLKSLAAGGA"
     gene            1022..1852
                     /locus_tag="VIS74_07875"
     CDS             1022..1852
                     /locus_tag="VIS74_07875"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY5793201.1"
                     /translation="MTSLSLITSCRNAVDLLPATLRSIRQQSLLLGRDARFEYIVVDG
                     ASTDGTVDLIRSAAAELPIRLISEPDRGVYDGLAKGFAKASGDVVGYLNAGDRLFPTA
                     LETLRDAFETTPASWMTGYSTLANAKGQPWRVNLPFRFRPQFFSNGLHGTFLLTLQQE
                     STFWRRSLLQGLNWDRFRSLKLAGDFYLWQHFSKTEPPAILHSLIGTFCAHPNQLSAD
                     NSQYVAELKSLCRAPRMSEWMVAWTDRFFWKAPRGVKRRLGGGRHIVYDPRADSWILK
                     "
     gene            1900..2532
                     /locus_tag="VIS74_07880"
     CDS             1900..2532
                     /locus_tag="VIS74_07880"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEY5793202.1"
                     /translation="MANPIRAVIFDMDGVLIDAKEWHYEALNRALGLFGFHINRYEHL
                     VTFDGLPTRAKLAMLTKDRGLPAGLHEVINGLKQKYTMEIVHRECKPLFQHEYALSRL
                     KAKGYKLAVASNSVRNSVEVMMQKSGLARFLDALVSNEDVKKGKPDPEIYLKTLDLLN
                     VSPGECLVVEDNENGIKSAQTAGCPVMIVRDVLDVTDERITQELSTLQNS"
     gene            2529..3266
                     /locus_tag="VIS74_07885"
     CDS             2529..3266
                     /locus_tag="VIS74_07885"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEY5793203.1"
                     /translation="MNLLVLMAGSHPSFEQTGHVFPRNLVQINDRPVLQHVMENLLPL
                     QSLGAKICALVSREEDRRFHTSRVLRLLNPEIAAQLVEGTTAGALCTALLAIGHIDRE
                     EPLIISNGDILMDCDLREAIRDFQERGLDGGAVVFRDIHPRWSFIKLDEAGFAIEAAE
                     KRPISHHATTGFYYYRRGGDFLEAAFRSLLKDAHVEGSFYICPVFNEMILDQKRVGVW
                     PIERSRYHTLKTAEDVRAYEERLTGVR"
     gene            3413..3748
                     /locus_tag="VIS74_07890"
     CDS             3413..3748
                     /locus_tag="VIS74_07890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5793204.1"
                     /translation="MTQANYETGRLEGMVRGWFVGDFSPTMLATGEVEVAIQRYPAGS
                     HEAAHFHKVATEITVILEGEAEMLGRIFRTGDIVKLPPGTTTDFKAITDVTTVVVKHP
                     GAKNDKYLA"
     gene            3745..4503
                     /locus_tag="VIS74_07895"
     CDS             3745..4503
                     /locus_tag="VIS74_07895"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEY5793205.1"
                     /translation="MNSPLNLVIPMAGRGSRFATAGFLDPKPMIPVGGRPMIEWVIEN
                     IRPARPHRFIFLCLGEHLVRYPQVPAKLRELCPGSEIISVDQVTEGAACTVLLARGLI
                     DSAAPLMIANSDQIVDLAIDEYLAAGDVPGVDGLIMTFWSDHPKWSFCRLRDGGDVSE
                     VVEKQVVSNDATVGIYNFRQGADFVRAADQMIARNLRVNGEFYVAPTYNQLIEEGRRV
                     VVKATGREGCGMYGLGIPEDLELFKQTDYYRKRL"
     gene            4531..5160
                     /locus_tag="VIS74_07900"
     CDS             4531..5160
                     /locus_tag="VIS74_07900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5793206.1"
                     /translation="MKILSHRGYWKTPAEKNSAAAFERSFALGFGTETDLRDRAGELV
                     ISHDLPRGGELTVREFLEIYSAFDRSLPLALNIKADGLQEPLRRLLEEFAVREYFLFD
                     MAIPDALVCLRSGLKCFTRQSEYEPSPAFYDQADGVWVDGFHGEWWDESLIRRHLEAG
                     KRACLVSPELHRRCHLPFWQSLRGWECRGNPALMLCTDLPEDARAFFEQ"
     gene            5225..6427
                     /locus_tag="VIS74_07905"
     CDS             5225..6427
                     /locus_tag="VIS74_07905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5793207.1"
                     /translation="MITGNVRSGREFRGIFEQVFELRRQGLVDGILFATWRDELRKFP
                     SLGKTLADQGVTTVAIEPPAALPAIHPLFHGYVLHQRKALHFALCSLPADCFVLKART
                     DFAEERFASMAQSLFREDSPSLRVNLPQPILQTRLFTFDARTDYFFYWDDIVFCGMRD
                     DLLALNNFELSPEVIYPAQLFAAEMRLFAPLFLKHYPVLAWFFENVHGEKFGRALQRW
                     AEAGDAVPLPVLAREILAAYFHILSRYLLLPEAAGELGGPLSLRQFFQDSPGRGVKRF
                     PTPWPSHKLVSAAPMRSLAEGRDGDDVEMAELLTRLERLDSDPAARRELRADLESRSG
                     ELVEFGRRFGEEISVCPVWRIPADSPAESPGLGSLELPEKRFLSRWGQRKLGVRRNLA
                     RWVLKNIL"
     gene            6436..>7311
                     /locus_tag="VIS74_07910"
     CDS             6436..>7311
                     /locus_tag="VIS74_07910"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY5793208.1"
                     /translation="MEIKPFLTWIVIPVHNRKALTLACLGHLRKTGVLDWAKVVVVDD
                     GSTDGTAPAIAGAFPEVVLLPGGGNLWWGGGIEKGMRFAHAAGAEAVIWLNDDTLPRP
                     GVLREMVELSLSRQANVTAATIQSETFSFGGQTQTRSGITPIDRCPPEPIECDTFHGN
                     LVCFPRQTIDRIGFIRGDLFPHTGGDSDYGLLARRQGIPNLYARNLVADLLEHERPQM
                     DSFLAGDTPLAVLWARVFRVGHGLYWRSNWWLRVRHWGAWGVWLFLKMYVRLLLLSLA
                     RVCLPKEFRSRLALRF"
BASE COUNT         1333 a         2110 c         2285 g         1583 t
ORIGIN      
        1 ccaatggcgc cgtgcgggcg ggccggcaaa aaccgcgccg ctggctcgaa gcgcttgccc
       61 gcgggctggg cgtgaaaatc tgaccggaca tttccatgag cgtttgggtg gtcattaaca
      121 attataacta cggacggttt ctcgccgacg ccctgcaaag cgttttggct cagacgcgtt
      181 tccccgaccg aatctgcgtg gtggacgatg gttcgacgga cgattcccgg cagctcctgc
      241 gaaaggagtt caaggatcga gtcgaggtcg tggaaaaacc aaacggcggg cagctttcct
      301 gttttaacga aatcgccgga cgagtagggg agaacgatgt gatctttttt ctggatgccg
      361 acgatcgttt ccggcccggc catctggaaa aatgcctggc cgtgtatgaa cagcaccccc
      421 aggtggattt tgtgtttacc cgccatcagc gtttcgggga ggaatccggt cccggcgtga
      481 ttttatgcga ccccctgaaa ggagatttgg gtttttccgt tttctccgcc cggaatgagc
      541 tggtctggcg gggcggtccc acctccacgc tttccctgcg ggggcggatc ttgcggcgtt
      601 tcctgccctg tcccttggaa aacgattggc gggttcgggc ggacgacgtg ctggtcttcg
      661 gctcctccct ggcgggggcg cgcaaatatt ttctcgatca gccgacggtg gactaccgcg
      721 tgcatgcctc caatcatttt cataaccgat catcggcgct tgaggattcc tttgcgtatg
      781 ttctgcgaag ggagcgtctt ctggcctggc tcgaaacgca cctgggcatg cccttccgcc
      841 gggaggcgat cctgattgct atcgaataca agtgccttcc gggagggaag gctgcggaat
      901 tttggcgtta tctgaaaatg gtggcagttt gccgttcgcc cctcccggaa aagggacgcg
      961 ctttgctttc cctgctgaag tcgctcgccg ccgggggtgc ctgaaaggtg gcatccgctt
     1021 tatgacctcc ctttcgctca tcacgtcctg ccggaacgcg gtggacttgc tgcccgccac
     1081 cttgaggtcc atccggcagc aatccctctt gctcggccgc gatgcccgtt ttgaatacat
     1141 tgtggtggac ggcgcctcga cggacggaac cgtggatttg attcgctccg ccgccgcgga
     1201 gctgcccatc cggctgatca gcgagccgga tcggggggtt tacgatggct tggccaaagg
     1261 ctttgccaaa gcctcgggtg acgtggtggg ttatctcaac gcgggtgacc ggcttttccc
     1321 cactgccctg gaaaccctgc gggatgcctt tgaaaccact ccggcttcct ggatgacggg
     1381 atactcgacc ttggcaaacg ccaaggggca gccgtggcgg gtgaatctgc cgtttcgttt
     1441 tcgcccgcaa tttttttcga atgggctgca cggaacgttt ctgctcaccc ttcaacagga
     1501 aagcaccttc tggcggagaa gtttgctaca ggggctgaac tgggaccggt ttcgcagcct
     1561 caagctcgcg ggcgactttt atctctggca gcatttcagc aaaaccgagc cccccgcgat
     1621 ccttcactcc ctgatcggaa ctttttgcgc gcatccgaac caattgagcg ccgacaattc
     1681 ccaatatgtg gccgagttaa aatcgctctg ccgagctccc cggatgtctg agtggatggt
     1741 tgcctggact gatcgttttt tttggaaggc cccgcggggc gttaaacgga ggctcggcgg
     1801 cggacggcat attgtctatg atccccgagc cgattcctgg atccttaaat aaggcttgcg
     1861 aaaagtggat aggctctctt tttccgtatt aactgtttta tggcaaatcc aatccgcgca
     1921 gtaatcttcg acatggacgg cgttttgatc gacgccaagg aatggcacta cgaggccctg
     1981 aaccgggcgc tgggcctctt tggatttcac atcaatcgct acgagcacct cgtgacgttc
     2041 gacggcctgc ccacccgggc caaactggcg atgctgacca aggaccgcgg cctgccggcc
     2101 ggactgcatg aagtcatcaa cggcctcaag cagaaataca cgatggaaat cgtccaccgg
     2161 gagtgcaagc cgctcttcca gcacgaatac gccctctcgc gattgaaagc gaagggctac
     2221 aagctcgcgg tcgcttcgaa ttccgtccgc aacagcgtgg aggtcatgat gcaaaaaagc
     2281 ggcctggcgc gctttttgga tgcgctggtg agcaatgagg atgtgaaaaa ggggaagccc
     2341 gatccggaaa tttacctcaa gacgctggat ctgctgaacg tcagtcccgg ggaatgcctc
     2401 gtggtggagg acaacgaaaa cggcatcaag tccgcccaga ccgccggctg ccccgtgatg
     2461 atcgtgcggg acgtgctgga cgtcaccgac gagcgcatca cccaggaact ttccaccctc
     2521 caaaactcat gaatctcctt gtgttgatgg ccggaagcca tccctccttc gagcagacgg
     2581 ggcatgtgtt tccgcgaaac cttgttcaga tcaatgaccg gccggttttg cagcacgtga
     2641 tggaaaacct gctgccgctg caatccctcg gcgcgaaaat ctgcgcgctg gtcagccggg
     2701 aggaagaccg gcgctttcac accagccggg tgctccgcct gctgaatccg gagatcgccg
     2761 cgcagctggt ggagggaacg accgccgggg cgttgtgcac cgcgctgctg gccatcggcc
     2821 acatcgaccg cgaggagccg ctgatcattt ccaatgggga catcctcatg gactgcgatt
     2881 tgcgggaagc gatccgggac tttcaggagc gcgggctgga tggaggcgcg gtggtgtttc
     2941 gcgacattca tccgcgctgg tccttcatca agctggacga ggcgggcttc gccatcgaag
     3001 ccgcggagaa gcgcccgatc agccatcatg ccacgaccgg tttttattat taccgccgcg
     3061 ggggggattt tctggaggcg gcattccgct cgctgttgaa ggacgcgcac gtggaaggct
     3121 cgttttacat ttgcccggtt tttaatgaaa tgatcctcga ccagaagcgc gtcggcgtct
     3181 ggccgatcga gcgctcccgg tatcacaccc tcaaaacggc cgaggacgtc cgcgcttatg
     3241 aggagcgctt gaccggagtg cgttagtggt tgtccacaga ttacgcagat aaaaatctcc
     3301 acaaaattcc ctgaaatctg cgtcatctgc gctgcaacgc acgcgaagcg gcggccaggc
     3361 tttcaaatcg gcggatgaga ttccgtttcc cctgaaaacc gccaatgacc ttatgacaca
     3421 agcaaactac gaaaccggcc gcctcgaagg catggtccgg ggttggttcg tcggagactt
     3481 ttcgccgacc atgctggcca ccggcgaggt ggaagtggcg atccagcggt atccggcggg
     3541 cagccacgaa gccgcgcatt ttcacaaggt cgccacggaa attaccgtga ttctcgaggg
     3601 ggaggccgag atgctgggcc ggattttccg cactggggac atcgtgaaac ttcctcccgg
     3661 cacgaccact gattttaagg cgattaccga tgtcaccacc gtggtggtga agcatcccgg
     3721 agccaaaaac gacaaatacc tcgcatgaac tcacccctga acctcgtcat tcccatggcc
     3781 gggcgcggct cccgcttcgc caccgcggga tttctggatc ccaagcccat gatccccgtc
     3841 ggcggccgtc cgatgatcga atgggtcatt gagaacatcc gccccgcccg cccgcaccgt
     3901 ttcattttcc tctgcctggg cgagcatttg gtgcggtatc cgcaggtgcc ggccaagctt
     3961 cgcgagcttt gcccgggcag cgagatcatt tccgtggacc aagtgacgga gggggctgcc
     4021 tgcaccgtgc tgctggcccg cggattgatt gactccgcgg ctccattgat gatcgccaac
     4081 agcgaccaaa tcgtggatct cgccattgac gaatatctgg ctgccgggga cgtcccggga
     4141 gtggatggcc tgatcatgac cttttggtcg gatcatccca aatggtcttt ttgccggctg
     4201 cgggatggcg gggatgtttc cgaagtcgtg gagaagcagg tggtcagcaa cgacgccacg
     4261 gtgggaattt ataattttcg ccagggggcg gacttcgtgc gcgcggcgga ccaaatgatc
     4321 gcccggaacc tgcgggtgaa cggtgagttt tacgtggctc ccacgtataa tcagctcatc
     4381 gaggaaggcc ggagagtagt ggtgaaggcc accggccggg aaggctgcgg catgtatggc
     4441 ctgggaatcc cggaggatct ggagcttttc aagcagacgg attattaccg gaagcgcctc
     4501 taaaaagcgg ccgcaccgga tcaggcaccc atgaaaattc tctcgcatcg cggttattgg
     4561 aaaactcccg ccgaaaaaaa ttccgcggcg gcctttgagc gcagctttgc attgggattc
     4621 ggaacggaga ccgacctccg ggaccgggcg ggggagctgg tcatcagcca tgacctgccg
     4681 cgcggcgggg aattgacggt gcgggagttc ctggaaattt acagcgcgtt cgatcgctcc
     4741 ctgccgctgg ccctcaacat caaggcggat gggttgcagg agccgctccg ccgcctgctg
     4801 gaggaattcg ccgttcggga atatttcctg ttcgatatgg caattcccga cgcactggtg
     4861 tgcctgcgga gcggtttgaa gtgctttacc cggcaaagcg aatatgagcc cagccccgcg
     4921 ttttacgatc aggccgacgg cgtttgggtg gacggttttc acggcgaatg gtgggatgaa
     4981 tccctcatca ggcggcatct ggaagccggc aagcgggcct gcctggtttc ccccgaactg
     5041 catcggcgct gccaccttcc cttctggcaa tcgctgcgcg gctgggagtg ccggggaaac
     5101 cccgccttga tgctttgcac cgatttgccc gaggatgcgc gggccttttt tgagcaataa
     5161 aaacgtccgt ttgggatcag aaatgttgcc tcctccttca ttatctcacc cgcccgtctg
     5221 ggtggtgatc accgggaacg ttcgctcggg gcgcgagttc cgggggattt tcgagcaggt
     5281 atttgagttg cgccgccaag gtttggtgga tggcattctc tttgcgactt ggcgggacga
     5341 acttcggaaa tttccgtcgt tgggcaagac tctggccgat cagggagtca ccaccgtggc
     5401 gatcgagccg ccggcggcgc tgcccgccat tcatccgctt tttcacggct acgtgcttca
     5461 tcaacggaag gcgctgcatt tcgcgctgtg ttccctcccg gcggattgct tcgtgctcaa
     5521 ggcgcggacg gatttcgcgg aggagcgttt tgcctcgatg gcgcaaagcc tgttccggga
     5581 agattctccc agcctgagag tcaacctccc gcagccaatc ctccaaacgc gtttgttcac
     5641 cttcgacgcg cggacggact atttcttcta ttgggacgac atcgttttct gcggcatgcg
     5701 cgacgacttg ctggcgctca acaattttga gctttccccc gaggtgattt atcccgccca
     5761 gctcttcgcc gccgagatgc ggttgtttgc gccgctgttt ttgaagcatt accccgtcct
     5821 cgcctggttt tttgaaaacg ttcacgggga gaaattcggc cgcgcgctcc agcggtgggc
     5881 cgaagccggg gacgcggtcc ccctgccggt gctggcccgg gagattctcg cggcgtattt
     5941 tcacatcctg agccgttacc tgcttctgcc ggaggccgcc ggcgaattgg gcggtccgct
     6001 ttcgctgcgg caatttttcc aggactcgcc cggccggggg gtgaagcgat ttcccacgcc
     6061 gtggcccagc cataaattgg tttccgcggc gccaatgcgg tccttggcgg agggccggga
     6121 cggagacgac gtggagatgg ctgagctgct tacccggctg gagcgcctgg attccgatcc
     6181 ggccgcgcgg agggaactgc gggcggactt ggaaagccgg agcggcgagc tggtggaatt
     6241 cggccgccgg tttggcgagg aaatcagcgt ctgcccggtt tggcggattc cggcggactc
     6301 gccggcggaa tcgccggggc tggggagcct ggagctgccg gaaaaacgat ttctttcgcg
     6361 ctgggggcaa aggaagctgg gcgtgcgccg gaaccttgcc cgttgggtgt tgaagaatat
     6421 cctctgagcc ctccaatgga aatcaaaccg tttttaacct ggatcgtcat tccggtccac
     6481 aaccggaagg ccctgacgct ggcctgcctg ggccatcttc ggaagacggg ggtattggat
     6541 tgggcgaaag tcgtggtcgt ggacgatggt tccaccgacg gaaccgcgcc ggcgattgcc
     6601 ggggcatttc ccgaggtcgt tctgctgccg ggcggcggca atctgtggtg gggcggcggc
     6661 attgaaaagg ggatgaggtt cgcccatgcc gccggagccg aggccgtaat ttggttgaat
     6721 gacgatacct tgccccggcc gggggttttg cgggaaatgg tggagctcag tctgtcccgg
     6781 caggccaatg tgacggcggc gaccattcag agcgaaacct tcagctttgg cgggcagacc
     6841 caaacccgga gcgggattac gccgattgac cgctgtccgc ccgagccgat cgagtgcgat
     6901 accttccatg gtaatctcgt ctgttttccc cggcaaacca ttgatcggat cgggtttatc
     6961 cggggcgatc ttttcccgca taccggcggg gattccgatt acggattgct cgcccgaagg
     7021 cagggaattc ccaaccttta tgctcgaaac ctcgtggcgg atcttctgga gcatgagcgc
     7081 ccgcagatgg attcgtttct ggcgggggat acgccgctgg cggtgctttg ggcgagggtt
     7141 ttccgcgtgg ggcacggcct ttactggcgc agcaattggt ggcttcgcgt gcgccattgg
     7201 ggcgcctggg gagtctggct gttcttgaaa atgtatgtcc gcctcctgct tctgagcttg
     7261 gccagagtct gcctgcccaa ggaattccgg tcgcggctgg ccttgcgatt c
//
LOCUS       DATDAY010000401         6764 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_1915542, whole genome shotgun sequence.
ACCESSION   DATDAY010000401 DATDAY010000000
VERSION     DATDAY010000401.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 6764)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6764)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6764
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1915542"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(198..569)
                     /locus_tag="VIS74_07915"
     CDS             complement(198..569)
                     /locus_tag="VIS74_07915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5793209.1"
                     /translation="MKTAIIILSDPNAGEEALGRAFNALAVAHEGLQAGDEVEIVFNG
                     AGARWPEQLTKLSHPANGLYNLVREAVKGVSCGCAAVFGATQGVEACGLPLITDKALP
                     GTPGISNIRRYLADGWQTLVF"
     gene            689..1042
                     /locus_tag="VIS74_07920"
     CDS             689..1042
                     /locus_tag="VIS74_07920"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY5793210.1"
                     /translation="MNDNEKNLLKRMPKLDSRQVDILLEDVLGCRWTISVLRAVARGV
                     KRPGALERHIDGISAKVLADRLRHFTRAGIFERVQFPEIPPRVEYQLTDFGKKFLRLL
                     KEVEKLQAELNGRAI"
     gene            complement(1035..2924)
                     /locus_tag="VIS74_07925"
     CDS             complement(1035..2924)
                     /locus_tag="VIS74_07925"
                     /inference="COORDINATES: protein motif:HMM:NF015585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HEY5793211.1"
                     /translation="MNFPLSSNFARGAATAAYLFKKAGGLAVFCVLGLSPATVSAAAA
                     NYADVYLFAGQSNMWGTGNAAADLPPELTGVQKDVMNFKNTPSVPKEGWYPLENGKNN
                     NHTGEAWGLEAQLMPALAAVADSKPIYMLKDSLGGAALAKKEMERDWSVDSQGELLDG
                     FLKSIQKVKASLEAEGKTPRFKGFIWMQGEAECCHAKADAENYQAMLEALIAKVRETA
                     GAPDMPVYLGRIHRQYAQPHLQIVRARQARAAATGKNIFLFDTDSAPLHGDKIHFNGA
                     GQVANGKTLFDLITGKAKQVPAKIVPGQAFSIREYAGPGTLVGKIALSQAGLPTPTFT
                     LESEAFEVNGETGEIKVRDLSGIKSFANALKVPVSAVNGARPTPAEAVTVNFQAAADD
                     KIAGLYAVMDPADAGSSLVVEGGYEEIRDLSDPSRKYKAPDAARRPLQEAFPGTAKQA
                     PRFAGKQIMMGPASNEFLEPGKDEDFTIAAAVHVPAHSLSSEGWCLMINKGVEPALGI
                     GYNYAQNQFFFVYSNPSNGTVQRVMADKETAPGASHVVRMRKAGETCRLYIDGALAGE
                     QRGKARPAILTPGQGAAIGMGGAFPPNPSFAGFLGKFYLVKGIPTTREEERIDRDLAE
                     WGGIK"
     gene            complement(3007..6294)
                     /locus_tag="VIS74_07930"
     CDS             complement(3007..6294)
                     /locus_tag="VIS74_07930"
                     /inference="COORDINATES: protein motif:HMM:NF018190.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HEY5793212.1"
                     /translation="MTFPTLFRVLFPLFATAAAIQAADPGNGDFENGLDGWKTEGSIS
                     ADSAEPFAGKSSLKLARDPLKVTLPTVVIGPSFPVTGARVEVSGAARSDLYFQDLSFN
                     GTLLVEFLGQDGKPLGRQSILSVHDRTPWKAFTKQVPMLKGSVQARLVAQLEKTHGSF
                     WLDDLTVKSIASATGLDALTRFSTKYLGNLFYPDDKVTIDLQVEPSAPLNAKAREVIC
                     TLTDYWGLPQHEPFTVTLAEKDGKYRAALDLSRLPLTVGKYYELQTRQDFGGAEPYRA
                     AMSFAILPEAATAKMDPFSVPFGTHTWDARLTEYFYLSARLGIRRCLVFWDWPQTPPH
                     TPHFPGWEYDVRLGHPKKSGMRPYGVLYPVSDVEHNEKQRGEEDLRAGIRQSIELFKK
                     DGLWGFQAGNEPPSWDPEMVKRNVELYRVVYEETKKTDPNFFVIGSAIGPEEQYFKLG
                     FGRYCDAYNVHSYGSLAELRRAMREYKEMFAKYGNAKPVYSTEIGSKSQGLSRHEIAM
                     DVVRKAVCFLADGGGFFTWFAVTYPDKDGTRRGSYGDSMDLYGGYLNMYLPRIDAVAY
                     YHIINAIGDRKFVQEKESADATSRFLFRNDRGEALQVLWNMKQTVDAFVPLPGVKAVR
                     LIHLDGSERTLDAGGKGVTLRVGEEPQLLLYSSAEKELAPELPPPALSLAELPASMVE
                     GNRAQITVQVAADEKGAVTLAPPPGWKTAPGRETPGANGRRTVVFDVEVPAGIEAREA
                     SMAILLQEKGREPGGELSFRVPLASMIEASIAPQAANAAQGEGRIQLTVTNRGGAAQA
                     VHWDVEILQEIPLKKGEFRFGDAETVKAHFTDVTEGSLNLGAGGEKHIEMKLAQVDPL
                     TIYRIQARARDAAGKSVITQRLVSGFVGVPHAPAGLKVDGHLDEPEWKKASAQAIHLP
                     RQFYQAEKGAEWSGPEDLSGTLRFLWDEKYLYVGVEVADDVFSNPKQDGNLWSQDGLQ
                     FLIDPARDRKEKKGRYDFSVGLGQKGPQAWSHLSGDQVRSPEGEIKDFQLAIRREGQP
                     KGHAVYEVAIPWTRIPPFQPAVGRDLGLSLILNEDDGKARFGFMAWFSGVHLKEMDHV
                     GDLILTK"
     gene            complement(6315..>6764)
                     /locus_tag="VIS74_07935"
     CDS             complement(6315..>6764)
                     /locus_tag="VIS74_07935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5793213.1"
                     /translation="GFAGNIEPDNSQAKSGSGSARIDAELAGVEESGLAWKSISKVID
                     IPGRIEAVRFWVKSAEARAISVRLSDSTGQTHSVRPDLAPGDDWQEVIVTDFESGPGH
                     LKYGGADDGKVHWPATSLTFVIEKGLLNDGRAIVWIDAIEILPGATP"
BASE COUNT         1215 a         2156 c         2042 g         1351 t
ORIGIN      
        1 aatcggtttc cgcttgccca agcgggtcgc gctcgggcgc aattgcgaca tgttgcgatt
       61 tgccgtagta atgctcctgg gctttatgca ccgaattcgt gaaggcgagc tcgagaaatt
      121 tgatggccat ggctggatag cgttgaatta ccgaggggga ggcagcgtgc agccgctccc
      181 cccacggctt ggatgtgtca aaagacaagc gtctgccagc cgtcggcgag gtagcggcga
      241 atgttggaaa tcccgggtgt tcccggcagg gctttgtccg tgatcaacgg aagtccgcat
      301 gcctcgacgc cctgggttgc tccgaacacc gccgcgcagc cgcacgacac gcctttcacg
      361 gcttcccgaa cgaggttgta gaggccgttg gccggatggg aaagcttggt gagctgttca
      421 ggccagcggg cgccagcccc gttgaatacg atttcgactt cgtcaccggc ctgtagtccc
      481 tcgtgggcaa cggcgagggc attgaatgcc cggccgaggg cttcttcgcc tgcgttggga
      541 tcggataaaa taatgatggc tgttttcatg atggttgtgt ttttttgtct tgccgcgggt
      601 tgatgacggc gcttccagcc tcgggtgttt gccggaattg cgaaagaggg cacaatcgcg
      661 tgggctaggc acaaatcggt gtaaattaat gaacgacaac gagaaaaatt tattgaagag
      721 aatgccgaaa cttgattccc gacaggtgga tatcctgctt gaagatgtcc taggctgccg
      781 ttggacgatc agtgttttgc gcgcagtcgc gcgcggtgtg aagcgccccg gagcgctgga
      841 gcgccacatc gacggaatca gcgccaaagt gcttgccgac cgtctccgcc acttcactcg
      901 ggccggaatc ttcgaacgtg tccaattccc ggaaatcccg ccacgggttg aatatcagct
      961 cacggacttt ggcaaaaaat tcctgcggct gttgaaagaa gtggaaaaat tgcaggctga
     1021 actgaacggg cgcgctattt gattccgccc cattcggcga gatcgcggtc gatgcgctcc
     1081 tcctcccggg tggtggggat gcccttgacg agataaaact tgccgagaaa tcccgcgaag
     1141 gacgggttgg gcggaaaggc cccacccatg ccgatcgccg cgccctgtcc gggggtcagg
     1201 atggcgggcc gggccttgcc ccgctgttcg cccgccaagg cgccgtcaat gtaaagccgg
     1261 caggtctcac ccgccttgcg catccggacg acgtgcgagg cgccgggcgc cgtttccttg
     1321 tcggccatca cccgctgcac ggttccgttg gacggattgg aatacacaaa aaagaattgg
     1381 ttctgcgcgt agttgtagcc gatcccaagc gcgggctcga cgcccttgtt gatcatcagg
     1441 caccagccct ccgaagacag cgaatgcgcc ggaacgtgca ccgcggcggc gatggtgaaa
     1501 tcctcgtcct tgccgggttc aaggaactcg ttgctggccg gccccatcat gatctgtttg
     1561 ccggcgaagc gcggcgcctg cttcgccgtc cccgggaatg cctcctggag cgggcggcgc
     1621 gcggcatcgg gggctttgta tttccggctc gggtccgaaa gatcgcgaat ttcctcgtat
     1681 ccaccctcca ctaccagaga actgccagca tcggcgggat ccatgacggc gtaaagcccg
     1741 gcgattttgt cgtccgcggc ggcttgaaag ttcacggtga cggcctcggc cggcgtggga
     1801 cgcgcgccgt tgacggcgga tacgggaact ttgagagcgt ttgcgaaact cttgatgcca
     1861 ctcagatccc ggactttgat ttcccccgtc tccccgttga cttcgaaggc ctcgctttcg
     1921 agggtgaacg tcggcgtggg aaggcccgct tgggaaagcg cgatcttgcc aaccagcgtg
     1981 ccgggtccgg catattcgcg gatggaaaag gcctggcccg gcacgatttt cgcgggaacc
     2041 tgtttcgcct ttcccgtgat caaatcaaag agggtttttc cgttggccac ctggcccgct
     2101 ccgttgaaat ggattttgtc gccgtgcagc ggagcggagt ccgtgtcgaa caggaaaata
     2161 tttttccccg tcgcggccgc gcgggcctgc cgggcgcgga cgatttgcaa atggggctgc
     2221 gcgtactgcc gatgaatgcg cccgaggtaa accggcatgt ccggagcgcc ggcggtctcg
     2281 cgcaccttgg cgatgagcgc ctcaagcatc gcctgataat tttccgcgtc cgccttcgcg
     2341 tggcagcatt cggcctcgcc ctgcatccag atgaaaccct tgaagcgcgg cgtcttgccc
     2401 tcggcttcca ggctggcctt cactttttgg atgctcttga gaaatccgtc gagcagttcg
     2461 ccctgcgaat ccacgctcca atcccgctcc atttcctttt tcgcgagcgc ggcgccgccc
     2521 aggctgtcct tgagcatgta aatcggcttg gagtccgcca ccgccgccag cgcgggcatg
     2581 agttgcgcct ccagtcccca ggcctccccc gtgtggttgt tgtttttgcc gttctcgagg
     2641 ggataccatc cttccttggg cacggagggc gtgtttttga aattcatcac gtccttttgc
     2701 acgccggtga gctccggcgg cagatcggcc gccgcgttgc cggttcccca catgttcgac
     2761 tggccggcaa aaaggtaaac gtccgcatag tttgcggccg cggcggaaac ggtggcgggc
     2821 gataaaccca gcacgcagaa aacggccagc ccgccggcct ttttgaagag ataagctgcg
     2881 gttgcggcgc cccgggcaaa attggaggaa agggggaagt tcatcggacg gagattaaag
     2941 ggaattttgt ggataggagt atatcatttt ctcgcgccgc cagagcctga cgggagaagc
     3001 atggcttcat ttcgtcagaa tcagatcgcc cacgtgatcc atttccttga ggtgcacccc
     3061 gctgaaccag gccatgaaac cgaagcgggc cttgccgtcg tcctcgttca gaatcaggga
     3121 cagccccagg tcccggccca cggccggctg gaagggcgga atccgggtcc agggaatggc
     3181 cacctcataa acggcatggc ccttgggctg gccctcccgc cggatggcga gctggaagtc
     3241 tttgatctcg ccttccggcg accgcacctg atctcccgag aggtggctcc atgcctgcgg
     3301 tcccttttgt cccaggccca cggagaaatc ataacgtccc ttcttctctt tccggtcgcg
     3361 ggccggatcg atcaggaatt gcaggccgtc ctgcgaccac aaatttccgt cctgtttcgg
     3421 attggagaaa acgtcgtccg ccacctccac cccgacgtag aggtatttct catcccagag
     3481 gaaacgcagc gttccgctca gatcctccgg tccgctccac tccgcgcctt tctccgcctg
     3541 gtaaaactgg cgcggcagat ggatggcctg cgccgaagcc tttttccatt ccggctcgtc
     3601 cagatggccg tccaccttga ggccggccgg agcgtgggga acgccgacga aacccgagac
     3661 gaggcgctgc gtgatcacgc ttttgccagc cgcgtcccgc gcccgcgcct ggatgcggta
     3721 aatcgtcagc ggatccacct gcgccagctt catctcgatg tgtttttccc cgccggctcc
     3781 gaggttcagg ctgccttcgg tcacatcggt gaagtgggcc ttgaccgtct cggcgtcgcc
     3841 gaaacggaac tcgcccttct tcagggggat ttcctgcaaa atctccacgt cccaatgaac
     3901 cgcctgcgcc gcgccaccgc ggttcgtcac cgtgagctgg atgcgtccct cgccttgcgc
     3961 ggcgttcgcc gcctgcggcg cgatggacgc ctcgatcatg ctcgccagcg gcacgcggaa
     4021 ggacagctcc ccgcccggct cccggccttt ttcctgcaac agaatcgcca tgctggcctc
     4081 gcgcgcctcg atgccggcgg gaacctccac gtcaaagacg acggtccgcc ggccattcgc
     4141 gcccggcgtt tcccggcccg gggccgtttt ccatcccgga ggcggcgcca gcgtaacggc
     4201 gcctttttca tccgccgcga cctgcacggt gatctgcgcc cgattgcctt ccaccatgct
     4261 cgccggcaat tccgccaggc tcaacgccgg gggcgggagt tccggcgcca gctctttttc
     4321 agccgaggaa tacagcagca attgcggttc ctcgcccacg cgcagcgtca cgcccttccc
     4381 acccgcgtcc agggtccgct cgctgccatc caggtggatc agccgcacgg ccttcacccc
     4441 gggcagcggc acaaaggcgt ccacggtctg cttcatgttc cagagcacct gcaaggcttc
     4501 gccgcgatcg ttccgaaaca aaaaccggct ggtcgcgtcg gcggattcct tttcctgcac
     4561 aaacttccgg tcgccgatgg cgttgatgat gtggtagtag gccaccgcgt cgatgcgggg
     4621 cagatacatg ttcagataac cgccatacag gtccatcgag tcgccgtagc tcccccgccg
     4681 cgtgccgtcc ttgtccggat aggtcacggc gaaccaggtg aagaatcccc cgccgtccgc
     4741 caggaaacac accgccttgc gcaccacatc catggcgatc tcgtgccgcg acaggccctg
     4801 gctcttcgag ccgatctcgg tcgaatacac cggcttcgcg ttgccgtatt tggcgaacat
     4861 ctccttgtat tcgcgcatcg cccgccgcag ctcggcgagc gaaccgtagg aatgcacgtt
     4921 gtaggcatcg caatagcggc cgaaacccag cttgaaatac tgctcctccg gcccgatggc
     4981 cgagccgatg acgaagaaat tcggatcggt ctttttggtc tcttcataga ccacgcggta
     5041 gagctccaca ttgcgcttca ccatttccgg gtcccaactc ggcggctcgt tccccgcctg
     5101 gaagccccaa agcccgtcct tcttgaacaa ctcgatcgac tgccggatgc ccgcgcgcaa
     5161 atcctcctcg ccgcgctgct tctcgttgtg ctcgacgtcg ctgaccggat acaggacgcc
     5221 gtagggccgc atgccggatt ttttcgggtg gcccaggcgc acgtcatatt cccaacccgg
     5281 aaagtgcggc gtgtggggcg gcgtctgcgg ccagtcccaa aaaaccaggc agcgccgaat
     5341 gcccaggcgg gcggaaagat aaaaatattc cgtcagccgg gcgtcccacg tgtgcgtgcc
     5401 gaacgggacg gaaaaaggat ccatcttcgc cgtggccgcc tccggcagaa tggcgaacga
     5461 catggccgcc cgataaggct ccgcgccgcc gaaatcctgc cgcgtctgca attcgtaata
     5521 tttccccacc gtcaggggca agcggctcaa atcaagcgcc gcccggtatt tcccgtcttt
     5581 ttccgccagc gtcaccgtga acggctcgtg ctggggcagg ccccagtaat ccgtcagcgt
     5641 gcagatcacc tcccgcgcct tggcattcag cggagccgac ggctcgacct ggagatcaat
     5701 cgtcaccttg tcatccggat aaaacaggtt tccaaggtat ttggtggaaa agcgcgtgag
     5761 ggcatcgagg cccgtggcgc tggcgatgct tttcaccgtc aggtcgtcca gccaaaaact
     5821 tccgtgcgtc ttctcgagct gggccaccag ccgcgcctga accgagccct tgagcatcgg
     5881 cacctgcttg gtaaaagcct tccagggcgt ccggtcatgc acgctcaaga tgctttgccg
     5941 gcccagcggc ttgccgtcct ggccgagaaa ctccaccagc agcgttccat tgaaggacag
     6001 atcttgaaaa tagagatcgc ttcgcgccgc cccggacact tccacccggg cgcccgtgac
     6061 cggaaaggac gggccgatca ccaccgtggg cagggtgacc ttgagcgggt cgcgcgccag
     6121 tttcagcgac gacttccccg cgaagggctc cgcggaatcc gcactaatgc tgccctcggt
     6181 tttccagcca tcgaggccgt tttcaaaatc gccattgccg ggatcggcgg cctggatggc
     6241 ggcggcggtg gcgaagaggg gaaaaagaac gcgaaacagg gtgggaaagg tcatgaaaag
     6301 ggaaaatggc ggattcaggg ggtggcgccg gggagaattt caatcgcgtc aatccagacg
     6361 atggcccgtc cgtcgttgag cagccctttt tcgatgacaa acgtcaggct ggtcgccggc
     6421 caatgcacct tgccgtcgtc cgctccgcca tatttcagat gaccgggacc cgattcgaaa
     6481 tccgtgacga tcacttcctg ccaatcgtct cccggggcga gatcgggccg gaccgaatgc
     6541 gtttgtccgg tcgagtccga gaggcggacg ctgatggccc gcgcctcggc gctcttgacc
     6601 cagaagcgca ccgcttcgat gcggccggga atgtcgatta ctttcgaaat gctcttccag
     6661 gccaggccgc tctcctccac gccggccagt tccgcatcga tgcgcgcgga gccggaaccg
     6721 cttttcgcct gggagttgtc gggttcgatg ttgccggcga atcc
//
LOCUS       DATDAY010000402         2028 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_236693, whole genome shotgun sequence.
ACCESSION   DATDAY010000402 DATDAY010000000
VERSION     DATDAY010000402.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2028)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2028
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_236693"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..860)
                     /locus_tag="VIS74_07940"
     CDS             complement(<1..860)
                     /locus_tag="VIS74_07940"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEY5793214.1"
                     /translation="MAFIPPYPTPHKTKSSFFLRFVRGWHSWLHVLFERSYSMKMGQL
                     RQPGRDVFMVNDPKWIRHIMVDQWAKYPKHEIMHRVLEPLLGVSIFTTNGEVWERQRR
                     LVDQGFQQARLQHVFPLMRKSIAGMLDRMDLAADGRSYEVDGEMTYITADIMFRTILS
                     EDLDQADAEVIYKAFLEFQGHAQRAVMLIMYRLPDYFPRRASKKCAKQIRTLISALIA
                     RRYEERDKGEKAEKMDILGAIMNATDPVKGDRFSYEETVDQICMLFLAGHETSASALA
                     WALYLLSHNPT"
     gene            complement(876..>2028)
                     /locus_tag="VIS74_07945"
     CDS             complement(876..>2028)
                     /locus_tag="VIS74_07945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5793215.1"
                     /translation="RTRRVHYISGFDPRGVRHYHQLYKGEAARQEPHLGTRLNVGARK
                     NLGRKVGAWDISARWEDRMVATEYQFMGWDDLVRQHWDSSLWQLFRRSVPVYLKYVLL
                     GGFNRVSEVSRGAFYTSVFPVFFLLILLVLIVGGGGLALAGIIHFGGNPLLGGAAVLA
                     LAAGIFLAGMRWGEKLGVLWILRTCVFVVTAGTRFLPELKKRTDQMAETILREQAARP
                     VDEVLIVGHSIGTILAVSVVARMLEQSELPVSPRFKFLTLGQCIPYVSVMPTAQSLRR
                     ELGVLARDPRIPWIDVTSPPDPLCFYQVDPIRVSGVATEFNDRPKRLVLRVFKMFTAG
                     AYSRLRLNKLRLHFQYLMASELASAYDYFRITAGPAPLEKSVSGMSGAG"
BASE COUNT          442 a          630 c          589 g          367 t
ORIGIN      
        1 gtagggttgt gcgatagaag atacagcgcc cacgccagcg cgctggccga agtttcatgg
       61 ccggccagga acagcatgca aatctgatcc accgtctcct cgtagctgaa acggtcgccc
      121 ttcaccggat ccgtcgcgtt catgatcgcg ccgaggatgt ccattttctc cgctttttcc
      181 cccttatccc gctcttcgta acggcgggca atcaacgcgg agatcagcgt gcggatctgc
      241 ttcgcgcact tcttgctcgc ccggcgcgga aaatagtccg gcaggcggta catgatgagc
      301 atgacggcgc gctgcgcgtg cccttgaaat tcgaggaagg ccttgtagat gacttccgca
      361 tccgcctggt cgagatcttc ggaaaggatc gtccgaaaca tgatgtccgc ggtgatgtag
      421 gtcatctcgc cgtccacttc gtaactccgg ccgtcggcgg ccaaatccat gcggtcgagc
      481 attccggcga tcgatttgcg catcagcgga aacacgtgct ggagccgggc ctgctgaaat
      541 ccctgatcga cgagccgccg ctgccgctcc caaacttcgc cgttggtggt gaaaatgctc
      601 acgccgagca acggctccag cacccggtgc atgatttcgt gtttcgggta tttggcccat
      661 tgatccacca tgatgtggcg gatccatttg ggatcgttca ccatgaagac atcgcgcccc
      721 ggctggcgca gctgccccat tttcatggaa taactgcgtt cgaacagcac gtgcagccat
      781 gaatgccagc cccgaacgaa ccggaggaag aacgaactct tcgttttgtg cggggtcgga
      841 taaggtggaa taaaggccat gaaatcaaaa aagtctcaac cggcgccgga catgccggaa
      901 accgattttt cgagcggtgc gggaccggcg gtgatgcgga agtaatcgta agccgaggcg
      961 agctcgctgg ccatcagata ttgaaaatgc aggcggagct tgttgagccg caggcgcgaa
     1021 taagccccgg cggtgaacat tttgaagact ctcaacacga ggcgtttggg ccggtcgttg
     1081 aactccgtgg ccacccccga aacgcgaatc ggatcgactt gataaaaaca gaggggatcc
     1141 ggcggggaag tcacatcgat ccagggaatc cgcggatccc tggccagaac gcccaattcc
     1201 cgccgcaagg actgggcggt gggcatgacg ctgacgtagg gaatgcattg ccccaaggtc
     1261 agaaacttga accgcggcga aactggcagc tcggattgtt ccagcatgcg ggccaccacg
     1321 gatacggcca gaatcgtgcc aatgctatgg ccgacgatca gcacctcgtc caccgggcgc
     1381 gccgcctgtt cccgcaggat ggtttccgcc atttggtccg tccgcttctt gagttcggga
     1441 agaaaccggg ttcccgccgt gaccacaaac acgcacgtgc gcagaatcca gagcacgccc
     1501 agcttttcgc cccagcgcat gccggcgaga aagatgcccg ccgccagcgc cagaaccgcc
     1561 gctccgccca ggaggggatt gccgccgaaa tggatgattc ccgccagcgc gaggccgccg
     1621 ccccccacga tcaagaccag caggatcagg agaaaaaaaa ccggaaaaac cgacgtgtaa
     1681 aaggcgcccc gggaaacttc gctcacgcga ttgaacccgc ccagcaagac gtatttgagg
     1741 taaaccggca cactccggcg aaacagctgc caaaggctgg agtcccagtg ctggcgcacc
     1801 agatcatccc accccatgaa ttgatactcc gtggccacca tccggtcctc ccagcgggcg
     1861 gaaatatccc aggcgcccac tttccgtccc aggtttttac gcgcgccgac attcagccgc
     1921 gttcccaagt gaggctcttg ccgggccgcc tcgcctttat agagctgatg gtaatgccgg
     1981 accccccgcg gatcgaaacc ggaaatgtaa tgcacccgcc gggtccgg
//
LOCUS       DATDAY010000403         3250 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_2448316, whole genome shotgun sequence.
ACCESSION   DATDAY010000403 DATDAY010000000
VERSION     DATDAY010000403.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 3250)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3250)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3250
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2448316"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(166..2061)
                     /locus_tag="VIS74_07950"
     CDS             complement(166..2061)
                     /locus_tag="VIS74_07950"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEY5793216.1"
                     /translation="MISSAAVQPPRWFNTVLWGNTVALSWMWGLGLFFSVQFTSQFGL
                     FGLLSFAIPNGLGLVIFGLLTHHLARREPGSESLAHFFTAWSRPLRLVFFLYQILAVT
                     LTIFAFIRYVWQPLNLQPDLLFLPLTLLVVLAASILFGEEFNIRRIKWSHGAIGLIAL
                     AAIVVLLFAPDFISSALPVAALRQLPTDDLNFWGYLIPICIGFLTGPWLDLQQWQRAI
                     QMHRERVSIAAGFICGAVEFFLLLVFHGTLALWALGETGTRFVRAGLDGHLYAQDVIQ
                     RLLYSNVETHPWIFGAYAVFLCACILTTLDSGYIALRWFLQTNVRASKNVIFSLIPTS
                     LLTSPIPVLAFCGVVALAGALLRLELEYFMIFYATFFVGYSVLAIVRSYQNRAVHAMP
                     QIKMFCIGGLAVVIFAYGYFLRYPAAMIIGSLLPLGYVLWLLIKPTADEELVSGASEL
                     EDTAENSAPVMRTGDAVSTRPAAAPAAQDNGSSSARPAPIHDVGGHFEGKWFVHSFVA
                     TYADTNSVGNVYFGMYAMWVGKTRELFFNRVMPKFNLKNTPFYILTRSFEHKFIRETR
                     EFETVSVRIRIASYNRKFVILEHEIFDSAGQALGKGKQSLIFVSSTDYRLLDLPAEVS
                     SAFLAHT"
     gene            2163..3065
                     /locus_tag="VIS74_07955"
     CDS             2163..3065
                     /locus_tag="VIS74_07955"
                     /inference="COORDINATES: protein motif:HMM:NF014759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HEY5793217.1"
                     /translation="MRLLLVDGHYYLYRSFFAIRGLSNSRGEPTNAIYGYTKALRKML
                     GDVKPDRVGVVWDRGLPARRVELQPAYKQNRAEMPEDLRRQEDPVRRLCECLGLMNLS
                     LPDTEADDLIASYALAAHREGAEVFIATSDKDIQQLVRPGLSIYATVKSAAGFALLGP
                     EEIRERWGVEPARIADVLCLTGDSSDNIPGVNGVGEKTAAALIRQFGSVGELLQRPEE
                     IANGKQRERVLAARDLIRDNREMVRLDDDLPLPVPWLDLEIRRKDQALVAFLRECEFK
                     SLLAEVERETGLGSAPPAQPELFA"
     gene            3062..>3250
                     /locus_tag="VIS74_07960"
     CDS             3062..>3250
                     /locus_tag="VIS74_07960"
                     /inference="COORDINATES: protein motif:HMM:NF013909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HEY5793218.1"
                     /translation="MKTTELAAYLDTLLRVREIRDYPNAFNGLQLENRGEVTRVAAAV
                     DACEAVIQRAGAAGADLLI"
BASE COUNT          678 a          927 c         1145 g          500 t
ORIGIN      
        1 gggggggagt gggaaccggg gtcggcgtgg gtttgggggt cggaaccggg gtggcggggg
       61 aagggctggg cgaaggcggg cccggcggct tgccagccgg tttgcaagcc gtcgcgccca
      121 aggcgatggc cagcgaaagc gccgcgactg tgcccgtttt ccgcatcaag tatgcgccag
      181 gaaggcggac gaaacttcgg cggggagatc aaggaggcga tagtcggtgg aggaaacgaa
      241 aatcaggctt tgtttgccct tgccgagcgc ctggccggcg gaatcgaaga tttcgtgctc
      301 caggatcacg aacttgcgat tgtagctcgc gatgcggatg cgcacgctga cggtctcgaa
      361 ctcgcgcgtc tcgcggatga atttgtgctc gaaggagcgc gtcaaaatgt aaaagggcgt
      421 attcttgaga ttaaatttcg gcatgacgcg gttgaagaac agctcgcggg ttttgccgac
      481 ccacatcgca tacatgccaa aatagacgtt gcccaccgag ttggtgtccg catacgtggc
      541 gacgaaggaa tggacgaacc atttgccctc gaaatggccg ccgacatcgt ggatcggcgc
      601 cgggcgggcg gagctgctgc cattgtcctg cgccgcgggg gcggcggccg ggcgggtgga
      661 aacggcgtct ccggtccgca tgacgggggc cgaattttcc gcggtatcct ccaattcgct
      721 cgcgccgctg accagctcct cgtccgccgt cggcttgatg agcagccaca gcacgtaacc
      781 caggggcagg agcgaaccga tgatcatggc cgccgggtaa cgcaggaaat acccgtaggc
      841 gaagatcacc accgccaggc cgccgatgca gaacatcttg atctgcggca tcgcgtgcac
      901 ggcgcggttt tggtagctgc gcacgatggc cagcacggaa taccccacaa aaaacgtcgc
      961 gtaaaagatc atgaaatact ccagctccag ccgcagcagc gccccggcca gggccacgac
     1021 gccgcagaag gccaggacgg ggatcggcga agtcagcagg ctcgtcggaa tcagggagaa
     1081 aatgacattc ttgctcgcgc ggacattcgt ctgcagaaac cagcggagcg cgatgtatcc
     1141 gctgtccagc gtggtcagga tgcacgcgca gaggaagacc gcgtaggcgc cgaagatcca
     1201 cggatgcgtc tccacattgc tgtagagcag gcgctggatc acgtcctggg cgtagaggtg
     1261 gccgtcgagt cccgcccgga cgaaccgcgt gccggtctcg ccgagcgccc acagggcgag
     1321 cgtgccgtgg aagaccagca gcaggaaaaa ctcaaccgcc ccgcagatga agcccgcggc
     1381 gatggaaacc cgctcgcggt gcatctgaat ggcgcgctgc cattgctgca gatcgagcca
     1441 cggcccggtg aggaaaccga tgcagatggg aatgaggtaa ccccagaagt tcaggtcgtc
     1501 ggtcggcagc tgccgcaggg cggccaccgg gagggcgctg ctgatgaagt ccggcgcgaa
     1561 caacaggacc acgatcgcgg ccagcgcgat gaggccgatc gccccatggc tccacttgat
     1621 gcggcgaatg ttaaactcct ccccgaagag gatggacgcc gccaggacga cgagcagcgt
     1681 gagcggcaga aacaggagat ccggctgcag gttcaacggc tgccacacgt agcggatgaa
     1741 ggcgaagatg gtgagcgtga ccgccaggat ttggtagagg aaaaacacca gccgcagcgg
     1801 ccgggaccag gcggtaaaaa agtgggccag cgactcggag cccggttcgc gccgggcgag
     1861 atggtgggtg agcaggccga agatgacgag cccgaggccg ttgggaatgg caaagctgag
     1921 caggccgaaa agtccgaatt ggctggtgaa ctgaaccgag aaaaacagcc caaggcccca
     1981 catccaggaa agagcgacgg tattgcccca aagaacggta ttaaaccagc ggggaggttg
     2041 gacggcggcg gaggaaatca tgatcaagaa aaggttgaat cattttgcag cgacccgcaa
     2101 gtgttccgaa gcagtcaaaa aaatattcgg cagcggcccg ccgcgggggc taggatgcgg
     2161 tcatgcgact gcttttggtg gatggccatt attaccttta ccgctcgttc ttcgccattc
     2221 ggggattgag caactcgcgc ggcgagccga ccaatgccat ttatggctac accaaagcgc
     2281 ttcgcaagat gctcggagac gtcaagcccg accgggtggg cgtggtgtgg gaccgcggcc
     2341 tgccggcgcg gcgggtcgag ctccagccgg cctacaaaca gaaccgcgcg gagatgccgg
     2401 aggatttgcg ccggcaggag gatccggttc gccgcctgtg cgaatgcctg ggcctgatga
     2461 atctcagcct gccggacacc gaggcggacg acctgatcgc gagctacgcg ctggcggcgc
     2521 accgcgaggg cgcggaggtg ttcatcgcga cctcggacaa ggacatccag cagctcgtgc
     2581 ggccgggcct gtcgatctac gcgacggtca aaagcgccgc cggcttcgcc ctgctggggc
     2641 cggaggaaat ccgcgaacgc tggggcgtgg agccggcgcg aatcgccgac gtcctctgcc
     2701 ttaccggcga cagctcggat aacattccgg gagtgaacgg agtgggggag aaaaccgcgg
     2761 cggcgttgat ccggcaattc ggttcggtgg gggaattgct ccagcgcccg gaggaaatcg
     2821 ccaacggcaa acagcgcgaa cgggtgctgg ccgcccggga cttgatccgg gacaatcgcg
     2881 aaatggtccg gctggatgac gatctcccgc tgccggtgcc gtggctggat ctggagatcc
     2941 ggcggaagga tcaggcgctg gtggcgtttc tgcgcgaatg cgaatttaaa agcctgctgg
     3001 ccgaggtgga gcgggagacg ggcctgggtt cggctccacc ggcgcagccc gaacttttcg
     3061 cgtgaagacg accgaactgg ccgcgtatct cgacaccctg ttgcgggtgc gcgaaatccg
     3121 ggattacccc aacgccttca atggccttca gctggaaaat cgcggcgagg tcacgcgcgt
     3181 ggcggccgcg gtggacgcgt gcgaggccgt gatccagcgg gccggggcgg cgggggcgga
     3241 tttattgatc
//
LOCUS       DATDAY010000404         4509 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_1376539, whole genome shotgun sequence.
ACCESSION   DATDAY010000404 DATDAY010000000
VERSION     DATDAY010000404.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 4509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4509
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1376539"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..185
                     /locus_tag="VIS74_07965"
     CDS             <1..185
                     /locus_tag="VIS74_07965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5793219.1"
                     /translation="QASGSGLQNLRRRLEAIYPGLHRFEIGESRSEVVALVEIRMNHD
                     HDRRLQDYELRQRMEP"
     gene            182..913
                     /locus_tag="VIS74_07970"
     CDS             182..913
                     /locus_tag="VIS74_07970"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY5793220.1"
                     /translation="MRKWRSLLIDDEAPARNHLRELLAAHPRVEILGEAASVPEAAAL
                     CQQHKHDLLFLDLQMPRGLGFDLLPLIHPVPEVIFVTAYNEYAVRAFEVGAVDYLLKP
                     IFADRLEIALARLEHHGAARPPETAAELPEDSPVFLRTPKGLRAVLMTHITHVTADGN
                     YTTIWLEDGASHLTERTMHEWESIFPATRFVRLDRSLFVNTNHLQELRILSRDAALLR
                     IKGCREEIKIGRAGRSRLRQLHLFR"
     gene            1089..1922
                     /locus_tag="VIS74_07975"
     CDS             1089..1922
                     /locus_tag="VIS74_07975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5793221.1"
                     /translation="MIKKPSIGAVLALAVGFGAAAPSTYAGTAAPAKEEVVIVEEEKP
                     AFSGWLSFDLNSHFISYGADVWASGTSWGRGVFNPSAELAWATPIPGLTGLVGTWWDV
                     NNNTKSAIGGYLQEVDIWAGLSYAYEDLSVTVLYQAWMYGGQTEQILDVILKYANNTF
                     LNPAITFHNRLDPGAAEGGGGKNGTFFVPNISYNLKLWKFTITPSAAIGFATDDFHGG
                     GGGYGYTALGLNASVPIPYLPGSWELHGGVTYYNTNDYVIPNNVADNFVTGNIGVKLS
                     F"
     gene            2052..3323
                     /gene="urtA"
                     /locus_tag="VIS74_07980"
     CDS             2052..3323
                     /gene="urtA"
                     /locus_tag="VIS74_07980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033219 - urea binding [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter substrate-binding protein"
                     /protein_id="HEY5793222.1"
                     /translation="MKLTQSLCALALGASTLLTAPSPLRAADDTVKVGVLHSLSGTMA
                     ISETSLRDVLIFAFDEINAAGGVLGKKIEPVVVDGASNWPLFAEKAKQLLEVDKVAVV
                     FGCWTSVSRKSVLPVFEKDNGLLFYPVQYEGEELSPNIFYTAEAVGQQAIPAVDYLLA
                     DGKKKFYLIGTDYVYPQTTNLVLLEYLLSKGVPLENIGGGLRKDEAGKVISAGKYTPF
                     GHTDYQQIVAEIKQFAASGDACVINTINGDSNVPFFKEFAASGLTAADCPVVSMSLSE
                     DELRSLPTKDLVGHLGCWAYFMSIDSPENDKFKKDFATWLKTSTVTGIEKSNRVTCSP
                     MVLSYDGVYLWKAAVEKAGSFDVDKVRKVLESGEITFTGPNGLNTMEKNHHTKKNTYI
                     GETKADGQFKIVEEYKQVYPEPFLKGTFVKQ"
     gene            3414..>4509
                     /locus_tag="VIS74_07985"
     CDS             3414..>4509
                     /locus_tag="VIS74_07985"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5793223.1"
                     /translation="MRVPILRRILAISLAAFAFGHAGEMEKLARENAAKAILAPETEQ
                     VTLVEGLAKTPDPALAAIFDAWQREQVFIYDAPDGARIPFQLAPKNEGSGELQALRID
                     NGEPLAGPDGAPLKFDEKALKPVETFSKLRRAMRATTDLLALSDPAPAIRQSAILKLG
                     MKQSAGALPLLNDRLEKETNKAVKAGILDAIAIINLNSPDPQQQIAALEQLKKSLSIP
                     SKELVAKMAESSSQPPKVRAAAAATLKALENHLFWVDFFGTMFRGISLGSVLLIVALG
                     LAITFGLMGVINMAHGEIMVVGGYATYLVQCVFGAGVALSPFGLALNIPGFNFDPNSG
                     LYQSYFLFALPVAFLSAAGVGILLERGVIQF"
BASE COUNT          887 a         1551 c         1248 g          823 t
ORIGIN      
        1 atcaggcctc cggctccggc ctgcagaacc tccgccgccg cctcgaagcc atctacccgg
       61 gcctgcaccg tttcgaaatc ggcgaaagcc ggtcggaagt cgtcgcgctg gtcgagatcc
      121 ggatgaacca cgaccacgac cggcggctgc aggattacga acttcggcaa aggatggagc
      181 catgaggaaa tggcggtcgc tgctcattga cgatgaagcc cccgcgcgca atcacctgcg
      241 cgaactgctg gcggctcatc cgcgcgtgga aattctcggc gaggcggcga gcgtgcccga
      301 ggcggccgcg ctctgccagc agcacaaaca cgacctgctg ttcctggatc tgcaaatgcc
      361 ccgcgggctg ggcttcgatc tgctgccgct cattcatccc gtcccggagg tgatctttgt
      421 caccgcgtat aatgaatatg ccgtccgcgc cttcgaagtc ggcgcggtgg attatctgct
      481 caagcccatc ttcgccgacc ggctggagat cgcgctggcc cggctggagc atcacggcgc
      541 ggcccgcccg cccgaaacgg ccgccgaatt gccggaggac tcgccggttt ttctccgcac
      601 ccccaagggc ctgcgcgccg tgctcatgac ccacatcacg cacgtcaccg cggacggcaa
      661 ctatacgacc atctggctgg aggacggcgc gtcccacctc accgagcgga cgatgcacga
      721 atgggaatcc atttttcccg cgacgcggtt tgtccgcctg gatcgctcgc tgttcgtgaa
      781 caccaaccat cttcaggaat tgcgcatcct ctcgcgcgac gccgccctgt tgcggatcaa
      841 gggctgtcgc gaagagatca aaatcggccg cgccgggcgt tcccggctgc gccagctgca
      901 cctttttcgc tagccgcgcg cctcccgatt cgcccgtttc ccgattcgcg gagccggctg
      961 cctgattcac ggatttttag ccgccgaatg gcatggctgg cgcgtcggct gctacgtcgc
     1021 tgcaactccg caatggcttt cgggccggtg cgggcaacga aaaaacaaac caaacaggga
     1081 aacaaaccat gatcaaaaaa ccatccatcg gcgccgtcct ggcgctggcc gtcggcttcg
     1141 gagccgcggc cccatccacc tacgccggaa cggcagctcc ggcgaaggag gaagtcgtga
     1201 tcgttgaaga ggaaaaaccc gccttctccg gctggctctc cttcgatctg aacagccatt
     1261 tcatctccta tggcgcggac gtctgggcaa gcggcaccag ctggggacgc ggcgtattca
     1321 acccctccgc ggaactcgcc tgggccactc cgattcccgg attgaccggg ctcgtcggca
     1381 cttggtggga cgtaaataac aacaccaaat ccgccatcgg cggctacctc caggaagtgg
     1441 atatctgggc cgggctttcc tacgcttacg aggatctgtc cgtcaccgtt ctctaccaag
     1501 cctggatgta tggcggccag accgagcaga tcctggacgt gattctcaaa tacgcgaaca
     1561 acacgttcct gaatcccgcg atcaccttcc acaatcgtct ggatccaggc gcggcggaag
     1621 gcggcggcgg caagaacggc accttcttcg tgccgaacat cagctataac ctcaagctct
     1681 ggaaattcac catcaccccg tcggcggcca ttggcttcgc cacggacgat ttccacggcg
     1741 gcggcggcgg ttacggctac acggcgctcg gcctcaatgc cagcgtgccc atcccgtatc
     1801 tcccgggcag ctgggagctt cacggcggcg tcacctacta caacaccaac gactacgtga
     1861 tcccaaacaa cgtggcggac aacttcgtca ccggcaatat cggcgtcaaa ctctcgttct
     1921 aatccggcaa accggcggag cccgcggctc gtgggcatca aagattccga accccggcat
     1981 cctttgtgct caccccaagg ccgcggctcc gcccgcctgc cccgctccaa tcctcaactc
     2041 ccattattcc catgaaactc acccaatcac tctgcgctct cgcgctcggc gccagcaccc
     2101 tgctgaccgc gccctccccg cttcgggcgg ccgatgacac cgtcaaggtc ggcgtcctcc
     2161 attcgctcag cggcaccatg gccatcagtg aaacctcgct tcgcgacgtc ctgatcttcg
     2221 ccttcgacga aatcaacgcc gccggcggcg tgctcggcaa gaagatcgaa cccgtcgtcg
     2281 tggacggcgc gtcgaactgg ccgctcttcg ccgagaaggc caaacaattg ctcgaagtgg
     2341 acaaggtcgc cgtcgtcttc ggctgctgga cttccgtcag ccgcaagtcg gtgctgcccg
     2401 tgttcgaaaa ggacaacggg ctgttgttct accccgtgca atacgaagga gaggaactct
     2461 cgccgaatat cttctacacc gccgaagcgg tcggccagca ggccattccg gccgtggatt
     2521 acctcctcgc cgacggcaag aagaagttct acctcatcgg caccgactac gtgtatccgc
     2581 agaccacgaa tctcgtgctg ctggaatacc tcctttccaa gggcgtgccg ctggaaaaca
     2641 tcggcggcgg cctgcgcaag gacgaagccg gcaaggtcat ctccgcgggc aaatacacgc
     2701 cgttcggcca caccgactac cagcagatcg tcgcggaaat caagcagttc gccgcctccg
     2761 gggacgcctg cgtgatcaac acgatcaacg gcgactcgaa cgttccgttc ttcaaggaat
     2821 tcgcggcgtc gggactcacc gcggccgatt gcccggtcgt ctcgatgagc ctttccgagg
     2881 acgagctgcg cagccttccg accaaggatc tcgtcggcca cctcggctgc tgggcttact
     2941 tcatgtccat tgattcgccg gaaaacgaca agttcaaaaa ggacttcgcc acctggctga
     3001 agacctccac ggtgaccgga atcgaaaaat ccaaccgcgt gacctgcagc ccgatggtgc
     3061 tctcctatga tggggtttac ctctggaagg ccgcggtgga aaaggccggc agcttcgacg
     3121 tggacaaggt tcgcaaggtg ctcgaatccg gcgaaatcac cttcaccggc ccgaacggcc
     3181 tgaacacgat ggaaaagaac caccacacca agaagaacac ctacatcggc gaaaccaagg
     3241 ccgacgggca gttcaagatc gttgaggaat acaagcaggt ttatcccgag cccttcctca
     3301 agggcacctt cgtcaagcag taaactttcc gcgggcgcat tgagagggag ccggcctagt
     3361 tccccggctc cctcttctgc cgccttgccc caacctcaac cctgattttt cccatgcgtg
     3421 ttccaatcct ccgccgaatc ctggcaatct cgctggccgc attcgcattc ggccatgccg
     3481 gtgaaatgga aaagctcgcc cgcgaaaatg cggcgaaggc cattctcgct cccgaaaccg
     3541 aacaggtgac gctcgtggaa ggcttggcca agacgcccga cccggccctc gccgccattt
     3601 ttgacgcctg gcagcgcgag caggttttta tttacgatgc gcccgacggc gcccgcatcc
     3661 cctttcaact cgctcccaaa aacgagggct ccggcgagct tcaagccctc cgcattgaca
     3721 atggcgaacc gctggccggc cccgatggcg ctccgctgaa gttcgacgaa aaggcgctga
     3781 agccggtcga gaccttcagc aagctgcgcc gggccatgcg cgccaccacc gatctcctcg
     3841 cgctgtccga tccggctccc gccatccgcc agagcgcgat cctcaagctc ggcatgaaac
     3901 aatccgccgg cgcgcttccg ctgctcaatg accgcctcga aaaagaaacc aacaaggcgg
     3961 tcaaggccgg catcctggac gccatcgcga tcatcaatct caactcgccc gatccgcagc
     4021 agcaaatcgc cgccttggag caattgaaga aatccctttc gattcccagc aaggaactcg
     4081 tcgccaagat ggcggaaagc agctcccagc cgcccaaggt tcgcgccgcc gcggccgcca
     4141 cgctgaaggc gctcgaaaat catctcttct gggtggactt cttcggaacc atgttccgcg
     4201 gcattagcct cggctccgtg ctcttgatcg tcgcgctcgg actggccatc acgtttggtt
     4261 tgatgggtgt catcaacatg gcccacggcg agatcatggt cgtcggcggc tacgcgacct
     4321 acctcgtgca gtgcgtcttt ggcgccggcg tcgcgctgtc gcccttcggc ctggcgctaa
     4381 acatcccggg gttcaatttc gatcccaact ccggcctcta ccaaagttac tttctcttcg
     4441 ccctgccggt ggcgttcctg agcgccgccg gggtgggcat cctgctggaa cgcggcgtca
     4501 tccaattcc
//
LOCUS       DATDAY010000405         3710 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_246726, whole genome shotgun sequence.
ACCESSION   DATDAY010000405 DATDAY010000000
VERSION     DATDAY010000405.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 3710)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3710)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3710
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_246726"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..807)
                     /locus_tag="VIS74_07990"
     CDS             complement(<1..807)
                     /locus_tag="VIS74_07990"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEY5793224.1"
                     /translation="MAGLIKILITLVVAAVILGGAAYIANELYFKPKKMDQLEREELA
                     RLAAEAAAPPPPDSTVPLFEKLRTTADLPALRSGLEEFLQTHPDSTKAAEARELLGET
                     NANLLFSSQPTPDKESYTVVKGDSLVKIASKYQTSAEMLMRTNNLLTHNLQIGQSLLV
                     PKFTPRIVVNRKTKTLTLLNGGAFFKEYALQAPRLPAAQTTKISDKIAIKDGKRVAFG
                     AKEFVDADRTLMLAPPAGMFIRAEAEPASGLMLPRADLEEIYTLVPIGTPV"
     gene            complement(875..1399)
                     /locus_tag="VIS74_07995"
     CDS             complement(875..1399)
                     /locus_tag="VIS74_07995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5793225.1"
                     /translation="MISPDDFQYALEHTRVVVQPEQRIETFGQTTFEFMIVSELMDKV
                     NQVRVRNGHINAERPRIVAPQHFQKMLLEGFGERAREFADLLETRGDLVKILRYGFQL
                     KKTDFSEEILHEPVESVVGRLEERLKAGSNPLTALIEGVDEAWEVCLLKFTTDLIQRS
                     AGENLGEWKRRGLI"
     gene            1479..2165
                     /locus_tag="VIS74_08000"
     CDS             1479..2165
                     /locus_tag="VIS74_08000"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HEY5793226.1"
                     /translation="MTVSRFDCIDLLMRYLALFALFASLLFTGCETLPLRTKYDQQAH
                     RPKNPDKVRVKVSLSKQMIYVLEGNRPLLVTAACVGTPANPTPKGNFEVYLKIRNKRS
                     GSYGFAAKGNQIIGTTRGKVPSGWSYVGYPMPFWVEFAPAYGFHLGWVHPIPKSHGCI
                     RLHANDASDFFALVKIGTPVNIATTQPEDQTLGKEHRRPMDYNDPDPPLTHMISKQYF
                     DNLPPPHLVD"
     gene            2174..3379
                     /locus_tag="VIS74_08005"
     CDS             2174..3379
                     /locus_tag="VIS74_08005"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HEY5793227.1"
                     /translation="MSATPTIPPPKRVNLRTPEVMEQVQAQVENHYRSQLVEQIRANG
                     GTLQLGQTTVRLAKQFGFCYGVERAIDLAYAAQKVFPEKRLFILGEIIHNPEVNQQIA
                     AIGVKNILAKDSPVGLDDLTAEDVVIVPAFGSEVETINRIRARGAQIVDTTCGDVMSV
                     YKRVKQNAAEQVTSVIHGKASHEETRATASRARGNGGGHYLIVLNLAETDEVCHYLSH
                     GGDREHFLKRFAGAYSPGFDPDIHLQRVGVANQTTMLRSETDQVQQRLRKAILERDGG
                     DSSNFRFFDTICGATQERQDALFRLLDEKLNLLLVVGGYNSSNTTHLVEIGSHYVPTY
                     FVRSAECLRTADEILHYDLRLKTEIPSRDWLPLDRPLRVGVTAGASCPNNLIAEVIER
                     MLALRGEKF"
     gene            3447..>3710
                     /locus_tag="VIS74_08010"
     CDS             3447..>3710
                     /locus_tag="VIS74_08010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5793228.1"
                     /translation="MFFTPRYLKEAKHYLQAAKKVRNYRRDLLPAVELEQLNEAIEAL
                     REAMRERRRAAVEPAVAKLDALLGKLVPPQPDSGWREQVEVFLV"
BASE COUNT          712 a         1189 c         1094 g          715 t
ORIGIN      
        1 cacgggcgtg ccgatgggga ccagggtgta aatctcttcg agatccgcgc gcggcagcat
       61 gaggccggag gccggttccg cttcggcgcg aatgaacatg cccgccggcg gcgccagcat
      121 caaggttcgg tcggcgtcca cgaattcctt cgcgccaaag gccacgcgct tgccgtcctt
      181 gatcgcgatc ttgtcgctga tcttcgtggt ctgcgcggcc ggcagccggg gggcttggag
      241 cgcgtattcc ttaaaaaacg cgccgccatt cagcagcgtg agcgtcttgg ttttccggtt
      301 cacgacgatc cgcggggtga atttgggaac caggagcgac tggccgatct gcaggttgtg
      361 agtcagcagg ttattcgtcc gcatgagcat ttcggcgctg gtctggtatt tcgacgcgat
      421 tttgaccagc gagtcgccct tgaccaccgt gtaggattcc ttgtccggcg tcggctggga
      481 ggagaacagg aggttggcat tcgtttcgcc gagcagttcg cgggcctcgg cggccttggt
      541 cgaatcggga tgcgtctgga gaaactcctc cagcccgctg cgcaatgccg ggagatcggc
      601 ggtggtccgc agcttttcaa agaggggaac cgtggaatcc ggcggcgggg gcgcggccgc
      661 ttcggccgcc aggcgcgcca gttcctcgcg ctccagctga tccatttttt tgggtttgaa
      721 atacagctcg ttggcaatat aggccgcgcc tcccaggatg accgcggcga ccacgagcgt
      781 gatcaaaatt ttgattaagc ctgccatgaa aaaataacgc cgtgcgcgcc tatgcaaaag
      841 aaaccatggg ttgatgctag tgcgattgcc gggcttagat caagccccgc cgcttccact
      901 ccccgaggtt ttccccggcc gaacgctgga tcagatccgt cgtgaatttc agcaggcaaa
      961 cctcccaggc ttcgtccacg ccttcgatca gcgcggtcag cggattgctg cccgccttga
     1021 gccgctcctc cagccgcccc accacggatt cgaccggctc gtggaggatt tcctcggaaa
     1081 agtccgtctt tttcaactgg aagccgtagc gcaggatttt caccagatcg ccgcgcgtct
     1141 ccagtaaatc ggcaaactcc cgcgcccgct cgccaaagcc ctcgaggagc attttctgga
     1201 aatgctgcgg cgcgacaatg cgcgggcgct cggcgttgat gtggccattg cgaacgcgca
     1261 cctgattcac cttgtccatg agttcgctga cgatcatgaa ctcgaaggtc gtctgaccaa
     1321 aggtctcgat ccgctgctcg ggctgcacca ccacgcgggt gtgctcgaga gcgtattgaa
     1381 aatcgtccgg tgaaatcatg cgcagagcat aacttagctc atcctctctg caacaagatc
     1441 gtgcgaaaat ccggcccccg caaattccca gcggcggatt gacagtttcc cggtttgatt
     1501 gcattgatct gctcatgcgt tatctcgccc tttttgccct gttcgccagc ctgctcttca
     1561 cgggctgcga aactctgcct ctgcgcacca aatacgacca gcaggcccac cgcccgaaga
     1621 accccgacaa ggtgcgggta aaggtttcgc tgtccaagca aatgatttac gtcctggaag
     1681 gcaatcgccc gctgctcgtg acggcggcct gcgtgggaac gcccgcgaat cccactccca
     1741 agggcaactt cgaagtgtat ctcaagattc gcaacaaacg ctccggcagc tacggttttg
     1801 ccgccaaggg caaccagatc atcggaacca cccgcggcaa ggttccctcc ggctggagtt
     1861 acgtggggta tccgatgccc ttctgggtcg agttcgcgcc cgcttacggt tttcacctcg
     1921 gctgggtgca tccgatcccc aagagccatg gctgcatccg ccttcacgcc aacgacgcct
     1981 cggatttttt cgcgctcgtg aagatcggca cgccggtcaa tatcgccacc acgcagccgg
     2041 aggaccagac gctgggcaag gagcaccggc ggccgatgga ttacaacgac ccggatccgc
     2101 cgctgaccca catgatctcg aagcaatact ttgacaatct gccgccgccg catcttgttg
     2161 actaattaat tccatgtccg ccaccccgac catcccgccg ccgaagcgcg tgaatctgcg
     2221 cacgccggag gtcatggaac aggtgcaggc ccaggtggag aatcattacc gcagccagct
     2281 cgtcgagcag atccgcgcca atggcggcac gctgcagctc ggccagacca ccgtgcgcct
     2341 ggccaagcag ttcggcttct gctacggcgt ggagcgcgcc atcgatctcg cctacgcggc
     2401 gcagaaggtg tttcccgaaa aacggctctt cattctcggc gaaatcattc acaaccccga
     2461 ggtcaaccag cagatcgccg ccatcggcgt gaaaaacatt ctcgcgaagg attccccggt
     2521 cggactcgat gatctcaccg cggaggatgt ggtgatcgtt cccgccttcg gcagcgaagt
     2581 cgaaacgatc aaccgcattc gcgcgcgcgg cgcccagatc gtggacacca cgtgcgggga
     2641 tgtgatgagc gtttacaagc gcgtgaagca aaacgcggcc gagcaggtga cttccgtcat
     2701 ccacggaaag gcctcccacg aggaaacccg cgccacggct tcccgcgcgc ggggcaatgg
     2761 cggcgggcat tatctcatcg tgctcaatct cgcggagacg gacgaggtct gccactacct
     2821 cagccatggc ggcgaccggg agcacttcct gaaacgtttt gccggcgctt attccccggg
     2881 attcgatccc gacatccacc tccagcgcgt gggcgtggcg aatcaaacca ccatgctgcg
     2941 cagcgagacc gatcaggttc agcaacggct gcgcaaggcc atcctcgagc gcgatggcgg
     3001 ggacagctcc aacttccgct ttttcgacac catctgcggc gccacgcagg agcggcagga
     3061 cgcgctcttc cgcctgctgg acgaaaagct caatctcctg ctggtggtcg gtggatacaa
     3121 cagctccaat acgacccacc tcgtggaaat cggctcccat tacgtgccga cctattttgt
     3181 gcgcagcgcc gaatgcctgc gcacggccga tgaaattctg cactatgatt tgcgcttgaa
     3241 gaccgagatt ccctcccggg actggctgcc gctcgaccgc ccgctccggg tcggcgtgac
     3301 cgccggcgcc tcgtgcccga acaacctcat cgccgaggtc atcgagcgaa tgctggccct
     3361 gcgcggcgag aagttttagc cgcctccgcg ggctttccga gtttcggatt tgccattgcc
     3421 ccgggaagcc cccaagttaa ccccgcatgt ttttcacgcc ccgttacctc aaggaagcca
     3481 agcattatct ccaagcggcg aagaaagtgc ggaactaccg ccgggatctg ctgccggcgg
     3541 tcgagctgga gcagttaaat gaggcgattg aagccctgag ggaagccatg cgcgagcgcc
     3601 gccgcgcggc cgtcgagcca gccgtggcca agctggatgc cctgctcggg aaactcgttc
     3661 ccccgcagcc ggacagcgga tggcgggagc aggtggaggt ctttctcgtg
//
LOCUS       DATDAY010000406         2057 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_1034304, whole genome shotgun sequence.
ACCESSION   DATDAY010000406 DATDAY010000000
VERSION     DATDAY010000406.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2057)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2057
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1034304"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1062
                     /locus_tag="VIS74_08015"
     CDS             <1..1062
                     /locus_tag="VIS74_08015"
                     /inference="COORDINATES: protein motif:HMM:NF014062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HEY5793229.1"
                     /translation="HIYMAPVSAIGAAAPVSGGGQEIPETMSAKIISYYSGYFRSAAE
                     KNGYNPDIAEAFINKKKEVKIGDEVINPVDSLLTLSAQAAVKKYNGKPLLADGIADSV
                     EALARQAGLGQAAVQKFEPSGFERIAFWITVFAPLFLAAGGLLAYLEFKSPGFGVAGV
                     LSAVCFLLFFAGHYVAGLTGFEVMAVFVLGLLLVLVEVIFFPGVVFVSVLGLGLMLGS
                     LFFAMVDFWPSRPLEFKVEQFSRAMIQFSIALVLTVFAGSLLARYFPSLPFFRRFLLT
                     AASPGGGSIPVPVAALERRLPELGETGRAVSILRPSGRAVFGDDELDVMTDGEFIEPG
                     AAVVLERREGAQLYVRRKR"
     gene            1167..>2057
                     /locus_tag="VIS74_08020"
     CDS             1167..>2057
                     /locus_tag="VIS74_08020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit alpha"
                     /protein_id="HEY5793230.1"
                     /translation="MNQVSVDKANELNGLYNPEFEHDACGVGFVAQVSGRRSNQILKI
                     GLRSICGLMHRGALDADAKTGDGAGVSTQIPYKIFAPEVSRLGHNLYKETDLAIGFTF
                     LPHDNAYAQARAKAIVAEVLQKRDLFLFGWREVPTNLRVLGEKAQSTMPRIEQVLIGK
                     PWGMAADDYERRLFLARNEIEDRAAADNIRNFYIPSFSHRTLVYKGLLISASLDKFYT
                     DLTNPDYETAVCVYHQRYSTNTFPTWPLAQPVRMMAHNGEINTVKGNRNWTQAREAEL
                     RADFWKEDIALLKPIVQPGGS"
BASE COUNT          356 a          593 c          666 g          442 t
ORIGIN      
        1 cacatctaca tggcgccggt gagcgccatc ggcgcggcgg cgccggtttc gggcggcggg
       61 caggagattc ccgagacgat gagcgcgaag atcatttcgt attattccgg ctatttccgc
      121 agcgcggccg aaaagaatgg ctacaacccg gacatcgccg aggccttcat caacaagaag
      181 aaggaggtca agatcggcga cgaagtgatc aaccccgtgg acagcctcct gaccctgagc
      241 gcgcaggcgg cggtgaagaa atacaatgga aaaccgctgc tggccgacgg aattgccgat
      301 tcggtcgagg cgctggcgcg gcaggcgggg ctggggcagg cggcggttca gaagttcgag
      361 ccgagcggat ttgagcggat tgcgttctgg atcacggtgt tcgcgccgct gttcctcgcg
      421 gcggggggct tgctcgcgta tctggaattc aagagccccg gcttcggcgt ggcgggcgtg
      481 ctttcggcgg tttgctttct gctgtttttc gccgggcatt atgtcgccgg cctgaccggc
      541 tttgaggtga tggctgtctt tgtgctgggc ctcctgctcg tgctggtgga ggtcattttc
      601 tttccgggcg tggtctttgt gtccgtgctc gggcttgggt tgatgctggg gtcgctgttt
      661 tttgcgatgg tggatttctg gccgtcgcgg ccgctggagt tcaaggtcga gcaattttcg
      721 cgggcgatga tccaattcag cattgccctg gttttgaccg tttttgcggg gtcgctgctg
      781 gcgcgttatt ttccatccct gccgtttttt cgccggtttt tgctgacggc ggcttcgccg
      841 ggcggcggtt cgattcccgt tcccgtggcc gcgctggagc gccggctgcc cgaacttggg
      901 gaaaccggcc gggcggtttc cattctgcgc cccagcgggc gggcggtttt cggggacgac
      961 gaattggatg tgatgacgga cggggaattc atcgagccgg gcgcggccgt tgtgctggaa
     1021 cgccgcgaag gcgcccaatt gtatgtccgc cggaaaagat gacttatttt tgttcggtgg
     1081 taatttgatc ttgtggaacg gaaattgctt ccgttgaatt ttgtctgccg ccttcacccg
     1141 gcaaacaccg aattgggaat ccgcttatga atcaagtatc cgtagataag gccaatgaac
     1201 tcaacggcct gtataatcca gaatttgagc acgatgcctg cggcgttggg tttgtcgccc
     1261 aagtcagcgg ccgccgctcg aaccagatcc tcaaaatcgg tctgcgttcg atttgcgggc
     1321 tgatgcatcg gggcgcgctg gatgcggatg cgaaaacggg cgacggcgcc ggggtgtcca
     1381 cgcagattcc ctacaagatt tttgcgccgg aagtcagccg gctcggccac aatctctaca
     1441 aggagacgga tctggccatt ggctttacct tcctgccgca cgacaatgcc tacgcgcagg
     1501 cgcgggcgaa ggccatcgtc gcggaagtgc tccagaagcg ggatctcttc cttttcggct
     1561 ggcgcgaggt gccgaccaac ctccgggtgc tcggggaaaa ggcccagtcc acgatgccgc
     1621 gcatcgagca ggtccttatc ggcaagccct ggggcatggc cgcggacgat tacgagcgcc
     1681 gcctgttcct cgcccgcaac gaaatcgagg accgcgccgc cgcggataat atccggaatt
     1741 tctacatccc gtccttctcg caccgcaccc tcgtttacaa ggggctgctc atctccgcgt
     1801 cgctggacaa attttatacc gacctcacca acccggacta cgagacggcg gtgtgcgtat
     1861 atcatcaacg ctacagcacg aacaccttcc cgacctggcc gctggcccag ccggtgcgca
     1921 tgatggcgca caatggcgag atcaacaccg tcaagggcaa ccggaactgg acgcaggccc
     1981 gcgaggcgga actccgcgcg gacttctgga aggaggacat cgccctgctc aaacccatcg
     2041 tgcagcccgg cgggtcg
//
LOCUS       DATDAY010000407         2941 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_2305039, whole genome shotgun sequence.
ACCESSION   DATDAY010000407 DATDAY010000000
VERSION     DATDAY010000407.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2941)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2941)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2941
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2305039"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..195
                     /locus_tag="VIS74_08025"
     CDS             <1..195
                     /locus_tag="VIS74_08025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5793231.1"
                     /translation="PDYAKEKVFSRFFSLPRPRTGAKSSGLGLCFVQEAVNLHGGTVS
                     LEGRRDLPGIVARLTLPAAG"
     gene            complement(199..1665)
                     /locus_tag="VIS74_08030"
     CDS             complement(199..1665)
                     /locus_tag="VIS74_08030"
                     /EC_number="1.11.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF012425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase"
                     /protein_id="HEY5793232.1"
                     /translation="MSDTPKTLTSASGAPIADNQNSQTAGPRGPVLMQDYWLIEKMAH
                     FNRERIPERVVHAKGSAAFGTLTITHDISKYSKAKIFSKIGGSTPVLLRFSTVAGERG
                     AADAERDVRGFALKIYTEEGNWDIVGNNTPVFFVRDPLKFSDFIHTQKRDPKTNLRNN
                     TAAWDFWSLSPESLHQVTILMSDRGLPKTMREMNGFGSHTYSFYNDAGERFWVKYHFK
                     TLQGHAHYTDEEAIEVVGKNRETSQDDLFHSIEAGNFPKWRFCVQIMPEKDAETCRWN
                     PFDLTKVWPHGDYPLIEVGVLELNRNPENYFAEVEQAAFAPANVVPGIAHSPDKMLQA
                     RILSYTDAHRYRLGVNYQHLPVNAPKCPVFNYHRDGAMRFDGNFGGAVNYEPNSFGGP
                     KECPAAKEPPLKISGDADRYNHRDGNDDYTQPGNLFRLFTPEQKERLFSNIARHMGAV
                     PREIQLRQICHFFRADPGYGIGVAKALGIDLAEFMPKA"
     gene            1735..2622
                     /locus_tag="VIS74_08035"
     CDS             1735..2622
                     /locus_tag="VIS74_08035"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY5793233.1"
                     /translation="MEMHQLRYFLAVAQAGSFTRAAAKCRVAQPSLSQQILKLESELG
                     EKLFERQRQRVTLTAVGERFRQRAERIQWETGEALREVRETQEQPRGKVELGVLPTIA
                     PFLLPDLLAEFGSAHSEIEVILHEETTSRLLRMVDSGEVHLAMVSLPIAGSRFQTRPL
                     FKEELLLALPRRHSLANRKAITLADLESERFIVMQESHCLGTQSLQFCHARGFSPQVS
                     CRSAQIETLQAMVKSGLGISLVPEMARRSAPKGIVYRSLSGAKPMRSIGLIWSKRLEV
                     PRAGRELAAFLEKKLRRTG"
     gene            2667..>2941
                     /locus_tag="VIS74_08040"
     CDS             2667..>2941
                     /locus_tag="VIS74_08040"
                     /inference="COORDINATES: protein motif:HMM:NF013015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5793234.1"
                     /translation="MTLHSLFIDFNSYFASVEQQERPHLRGQPVAVVPVEAETTCCIA
                     ASYEAKRFGVRTGTRVSDARKMCPGLRVVHARPEVYVRFHERLLEEV"
BASE COUNT          520 a          857 c          977 g          587 t
ORIGIN      
        1 cccgactacg cgaaggaaaa ggtgttcagc cggttctttt cgctgccgcg cccccgcacc
       61 ggcgcaaaaa gttccggtct cggcctctgt tttgttcagg aagccgtgaa cctccacggc
      121 ggaacggtga gcctggaagg ccgccgggat cttccaggca tcgtggctcg tttgacgctg
      181 ccggcggcgg gataagacct atgccttcgg catgaactcc gcgagatcga tgcccagcgc
      241 cttcgcgacg ccgatgccat acccgggatc cgcgcggaag aagtggcaga tctggcggag
      301 ctgaatctcc cgcggcacgg cgcccatgtg gcgggcgatg ttgctgaaga ggcgctcctt
      361 ctgctccggt gtgaacaggc ggaacaaatt ccccggctgc gtgtagtcgt cgttgccgtc
      421 gcggtgattg taacggtccg cgtcgccgga aatcttgagc ggcggttcct ttgcggcggg
      481 gcattctttc gggccgccga agctgttggg ctcgtagttc accgcgccgc cgaagttgcc
      541 gtcgaaacgc atggcgccat cgcgatggta gttgaacacc gggcacttcg gcgcgttgac
      601 cggcaggtgc tggtagttca ctcccagacg atagcggtgc gcgtccgtgt aggaaagaat
      661 gcgcgcctgc agcatcttgt cgggcgaatg cgcgatgccg ggaaccacgt tggccggcgc
      721 gaacgcggcc tgctcgactt ccgcgaaata attttccgga ttccggttga gttcgagaac
      781 cccgacctcg atgagcggat aatccccgtg cggccagacc ttggtgagat cgaacggatt
      841 ccagcggcag gtctccgcgt ccttctccgg catgatctgc acgcagaagc gccacttggg
      901 gaagttgccg gcttcgatgc tgtggaaaag atcgtcctgc gaggtttccc ggtttttgcc
      961 aacgacctcg atggcctcct cgtcggtgta atgcgcatgg ccctgcaggg ttttgaagtg
     1021 atatttcacc caaaagcgct cgccggcgtc gttgtagaag ctgtaggtat gcgagccgaa
     1081 gccattcatt tcgcgcatgg ttttgggcag gccgcggtcg ctcatgagaa tggtgacctg
     1141 atgcagcgat tccggcgaga gcgaccaaaa atcccacgcg gcggtgttgt tgcgcaggtt
     1201 ggttttcgga tcgcgcttct gcgtgtgaat gaagtcgctg aacttgagcg gatcgcgaac
     1261 gaagaacacc ggcgtgttgt tgccgacgat gtcccagttg ccttcctccg tgtaaatttt
     1321 cagcgcaaaa ccgcgcacgt cgcgttccgc gtccgccgcg ccgcgttcgc ccgcgaccgt
     1381 cgagaaacgc agcagcaccg gggtggagcc gccgattttc gagaaaatct tcgccttcga
     1441 atatttcgag atgtcgtggg tgatggtcag cgttccaaac gccgcggagc ccttggcgtg
     1501 gaccacgcgc tcgggaatgc gctcgcggtt gaagtgggcc atcttttcga tcagccagta
     1561 gtcctgcatc aaaaccggcc cgcgcggccc ggcggtctgg ctgttttggt tgtcggcgat
     1621 tggcgccccc gaggcgctgg tcagagtttt gggtgtgtcg gacatgggat caccttaccg
     1681 gcttgagaat aggatggaaa tataaccttc ctatgcgtca tataggtgct tcctatggaa
     1741 atgcaccagc tccgctattt tctcgcggtg gcccaggccg ggagcttcac ccgcgcggcc
     1801 gcgaagtgcc gggtggccca gccgtccctg agccagcaga ttctgaagct ggagtccgaa
     1861 ttgggggaaa agctcttcga acgccagcgc cagcgggtca ccctgacggc ggtgggggaa
     1921 cggttccggc agcgggccga acggattcaa tgggaaaccg gcgaggcgct gcgggaagtc
     1981 cgcgagaccc aggaacaacc ccggggcaag gtggaactgg gagtgcttcc caccatcgcg
     2041 ccctttttgc tgccggactt gctggcggag ttcgggtcgg ctcattccga gatcgaggtg
     2101 attctgcacg aggaaaccac gtcgcgcctg ctgcgaatgg tggacagcgg cgaggtgcat
     2161 ctggcgatgg tgagcctgcc gattgccgga agccggtttc aaacccggcc gcttttcaag
     2221 gaagaactct tgctcgcgct gccgcggcgc cattcattgg ccaaccgcaa agccatcacg
     2281 ctggcggatc tggagagcga gcgttttatc gtgatgcagg aaagccattg cctcgggacg
     2341 caatcgctgc agttctgcca cgcgcgcgga ttttcgccgc aggtcagctg ccgcagcgcc
     2401 cagatcgaga cgctgcaagc gatggtcaaa tccggcctgg gcatttccct cgtgccggaa
     2461 atggcgcggc gctccgcgcc gaaggggatc gtttatcgct ccctgagcgg ggccaagccg
     2521 atgcgttcca tcggattgat ctggagcaag cgcctggagg tgccgcgggc cggccgggaa
     2581 ctggcggctt tcctggagaa gaaactgcgg cgaaccggct gaatggcatc gccggaaagc
     2641 cgggtttggg gtaagctaaa cccggcatga cgctgcactc gctgttcatt gattttaact
     2701 cctacttcgc ctcggtggag caacaggagc ggccgcattt gcgcggccag ccggtggcgg
     2761 tcgtgccggt cgaggcggag acgacctgct gcatcgcggc gagttatgag gccaagcgct
     2821 tcggcgtgcg caccgggacg cgcgtttcgg acgcgcggaa aatgtgccca ggattgcggg
     2881 tcgtccacgc gcgccccgag gtgtatgtga ggtttcacga acgcctgctg gaggaagtcg
     2941 a
//
LOCUS       DATDAY010000408         5235 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_250045, whole genome shotgun sequence.
ACCESSION   DATDAY010000408 DATDAY010000000
VERSION     DATDAY010000408.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 5235)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5235)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5235
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_250045"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1075
                     /locus_tag="VIS74_08045"
     CDS             <1..1075
                     /locus_tag="VIS74_08045"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEY5793235.1"
                     /translation="MDPSDAPGRTDYRALRDRMGADPFRRRIEKQAGIWARETHQGRG
                     IFYLERFVPMDEILARCLRGSGLAGWGRRNMLDLELVRREAIFPHLPPEWDGFRLLQL
                     ADLHLDLAPELTPRILEKLRGVEFDQAVLVGDYHNRIGTEAKVSLGEMRKLIPALGPK
                     PLGILGNHDFIEKVSFLEHCGLRILLNETVALERGGSRLWVAGVDDPHFFRTHDLEAA
                     RRPIPPGEFSILLSHSPETYREAAALGFDFMLSGHTHGGQICLPGGWPIIRNADVPRR
                     LLSGPWREGKMQGYTSRGTGACGVAARFFCRPEITLHTLRRELGANPDMVGGQSIETG
                     KPAPAGQAIPLVRPRSSDLNRRT"
     gene            1080..3236
                     /locus_tag="VIS74_08050"
     CDS             1080..3236
                     /locus_tag="VIS74_08050"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY5793236.1"
                     /translation="MTEKASDSPKPDGWPEFREELAKSNLQRMWWLLIASTSLGAGFA
                     LYHFWAAPKSAFVPWQVFDLGGSLIFLALIFLGRRGRLPPRARRALTPACFAFWLILM
                     DGYYFLALPVYGETATYALGVVAPAILILLPPRGFLSLLVPNHLVFCVLLLAAGHSRH
                     GYADALLGSLVNGTLGTIVAILAAWYRFAGTRRLFLREQELALRTQEASVAESHLRAI
                     LENIPFQAWLKDAGNVFLSVNLEFARAHGLRESDIVGKSTWEIYPPQRAQKYVREAEI
                     IMGTRQQKFFEDFLWEGGKRHWFEVFISPVIDGNGCCIGTAGLARDITERREMQEQLI
                     AADNAKSEFLAMMSHEIRTPMNSVLGYAQLLREMPLTPQQREYVDSINNSGKLLLAII
                     NDILDFSKIEAGKVALQAETVPLRALFQRVLGMFEPLAAGKKLALHLAIAGEVPEQVR
                     LDPHRLEQLLVNLLSNALKFTDAGFVELRVSAEEERRTVDGLIWRLRLEVTDTGIGIA
                     SQQMERLFRPFSQLESNAARGLTGTGLGLVIVEHLCKLMGGEIRVKSQPGAGSTFTAI
                     LRAAAPRLEAKGEPEPAPSPMPLGLAGRRVLVVEDNLVNRRLAAAILGRWDLQVDLVE
                     SGIQALEQIRHTIYDAVLMDVQMPGMDGLETTRLIRQWEAANPERPRLFIAALTAFAR
                     ADDSQRCLAAGMDDYLSKPLNPEALKQLLSQALRAK"
     gene            complement(3658..>5235)
                     /locus_tag="VIS74_08055"
     CDS             complement(3658..>5235)
                     /locus_tag="VIS74_08055"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-guanine transglycosylase"
                     /protein_id="HEY5793237.1"
                     /translation="CVPSTSDEATARAAVEITQRWAARSLAARGESPQALFGILQGAL
                     FPELRRQSAEGLLEMPFDGLAIGGLAVGETKSEREDLCEFSAALIPEEMPRYLMGVGT
                     PLDILEAVYRGVDMFDCIIPTQVAQRGGVFTSRGYLQLRRAVYKFSEEPLDPNCGCPT
                     CSRYSRAYLHHLTKTKETLGWQLLGKHNLHFYHQLMREIRASILADRFLELYHAKRTF
                     LQEDDLDNPTTAVKPRIVRKPRLGDYEIHTAREGFASILHVSSGEIMHSRTPPMDEAR
                     RLYIEQSRLAERVRPEAGAEPLVVWDVGLGAAANAMGAILCYEAQAAAGPVRPMRIVS
                     FENDLDSLRLAFKHNDRFPYLRHGGPAGILKQGHWQSKTHPGLSWTLIPGSFPETLAQ
                     ADSAPEVIFYDMFSSKTHGEAWTLAAFRQLFASCSGKAAELFTYTISTAARSALLAAG
                     FWVARGRSTGDKEETTIALTPAARSGLFGQRHELLGADWLKKWHRSGARIPAWVLPDE
                     RAAFEQAILGHPQFGEG"
BASE COUNT          887 a         1677 c         1718 g          953 t
ORIGIN      
        1 gatggacccg agtgacgctc ccggccgcac cgattaccgg gcgctgcgtg accggatggg
       61 ggccgatccc ttccggcggc ggatcgaaaa gcaggccggc atctgggcgc gcgagacgca
      121 tcagggccgg ggcatctttt acctggagcg ctttgttccg atggatgaga tcctcgcgcg
      181 gtgcctgcgt gggtcgggtt tggccggctg gggccggcgc aacatgctgg atctggagct
      241 ggtccggcgg gaagcgattt tcccccatct tccgccggag tgggacggct ttcggctgtt
      301 gcagctggcc gacctgcatc tcgatctggc tccggaactc acgccgcgaa ttttggagaa
      361 gctgcgcggc gtggagttcg atcaagccgt gctcgtgggc gactaccaca accgcatcgg
      421 cacggaggcg aaggtgtccc tgggcgagat gcgcaagctc attccggcgc tgggtccgaa
      481 gccgctgggc attttgggca atcatgattt tatcgagaag gtttccttcc tcgaacattg
      541 cggcctgcga atcctgctga atgaaaccgt ggcgctggag cggggcggct cgcgcctctg
      601 ggtcgcgggg gtggacgatc cgcacttctt tcggacccat gatctcgaag cggcgcggcg
      661 cccgattccg ccgggggaat tttcgatctt gctcagccac tcgcccgaga cctaccggga
      721 ggcggccgcg ctcgggtttg atttcatgct ctccggccac acccacggag gtcagatctg
      781 cctgcccggc ggctggccga tcattcggaa cgccgacgtg ccccgccggc tgctcagcgg
      841 cccctggcgg gagggaaaaa tgcaaggtta cacctcccgc ggcaccgggg cgtgcggcgt
      901 ggcggcgcgc tttttctgcc ggccggaaat caccctgcac acgcttcgcc gcgaattggg
      961 ggcgaatccg gatatggttg gcggccagtc aattgaaacc ggcaagccgg cgcccgccgg
     1021 ccaggcgatt ccgcttgttc ggccgcgctc aagcgattta aatcgccgca cttgattgaa
     1081 tgaccgagaa agcgtccgat tccccaaagc ctgacgggtg gccggaattc cgggaggagc
     1141 tggccaagtc caatctccag cgcatgtggt ggctgttgat cgccagcacg tcgctgggcg
     1201 ccgggtttgc cttgtatcat ttctgggccg ccccgaaatc ggcgtttgtt ccctggcagg
     1261 tttttgacct gggcggttcg ctgatttttt tggcgctgat ctttctcggc cgccgcggca
     1321 gattgccgcc gcgggcgcgg cgggcgctga cgccggcttg cttcgcgttt tggctgatct
     1381 tgatggatgg ctactatttt ctggccctgc cggtctatgg cgaaacggcc acttacgcgc
     1441 tgggcgtcgt ggcgccggcc atcctgatcc tgcttccgcc gcggggcttc ctcagccttt
     1501 tggtgccaaa tcatctggtc ttctgcgtcc tgctgctggc cgccggccat tcgaggcatg
     1561 gctacgcgga tgcgcttctg ggttcattgg tgaacggaac gctgggaacg atcgtggcga
     1621 tcctggcggc gtggtatcgt tttgcgggaa cgcgccggct ttttctccgg gaacaggaac
     1681 tcgccctgcg cacccaggaa gccagcgtgg cggaatcgca cctccgcgcc atccttgaaa
     1741 atatcccctt ccaagcctgg ctcaaggatg ccggcaatgt ctttctttcc gtcaacctcg
     1801 agtttgcccg cgcccacggg ctgcgcgaat cggacatcgt gggcaagtcc acctgggaaa
     1861 tttacccgcc ccagcgcgcc cagaaatacg tccgggaggc ggaaatcatc atgggaaccc
     1921 ggcagcagaa gttcttcgag gattttttat gggagggcgg aaagcggcac tggtttgaag
     1981 tattcatcag cccggtcatt gacgggaacg gctgctgcat cggcaccgcc ggcttggcgc
     2041 gcgacattac cgaacggcgg gaaatgcagg agcagctcat cgccgccgac aacgcgaagt
     2101 cggagttcct ggcgatgatg agccacgaaa tccgcacgcc catgaacagc gtgctcggct
     2161 acgcgcagct cctgcgggaa atgccgctca ccccgcagca gcgcgaatac gtggactcca
     2221 tcaataacag cggcaagctc ctcctggcga tcatcaacga cattctggac ttttccaaaa
     2281 tcgaagccgg caaggtcgcc ctgcaggcgg aaaccgtccc gttgcgggcc ttgttccagc
     2341 gggttttggg catgttcgaa ccgctggccg ccgggaaaaa gctcgcgctc catctggcca
     2401 tcgccgggga agtccccgaa caggtccggc tggatccgca tcggctggaa caattgctgg
     2461 tcaacctgct ttccaacgcc ctgaagttta ccgatgccgg cttcgtcgag ctgcgggtct
     2521 cggcggagga ggaaaggcgg acggtggacg gcctgatctg gcggctccgg ctggaagtca
     2581 cggatacggg catcggcatt gccagccagc aaatggagcg cctcttccgg ccgttcagcc
     2641 agctggaaag caatgccgcc cgcggcctca ccggcaccgg cctgggcctc gtcatcgtcg
     2701 aacatctttg caagctcatg ggcggggaga tccgggtgaa aagccagccg ggagccggtt
     2761 cgaccttcac cgcgatcctt cgggccgccg cgccccggtt ggaagcaaag ggcgagcccg
     2821 aacccgcccc ctcgccgatg cccctgggcc tcgccggccg acgcgtgctc gtggtggagg
     2881 ataatctcgt caaccgccgc ctggcggccg ccattctcgg gcgatgggat ctccaggtgg
     2941 acctcgtcga gagcgggatc caggcgctgg agcaaattcg ccataccatc tacgatgcgg
     3001 tattgatgga tgtccagatg cccgggatgg atggtttgga aaccacccgc ctcatccgcc
     3061 aatgggaggc ggcgaatccg gaacggcccc ggcttttcat cgcggccctg accgcctttg
     3121 cccgggccga cgacagccag cgctgcctgg ccgccggcat ggacgactac ctttccaagc
     3181 ccctcaaccc agaggcgctc aagcagttat tgtcccaggc gctgcgggcg aagtaggccg
     3241 cgcccgatcc atcagcaagc aacggacggc tcggcgagcc gtccctgccc atggataggc
     3301 agggcggttt gaaagcctgg cctcggcggc tttgccgcct gcgttgcagc gatgaaaccg
     3361 ccgtggcctt cctcgcaaga tttacgttca tgtaaattat agagcgattc tcaaaagaga
     3421 tttccgaaat gcgattcaat gcaggggcag ccggagcgaa gacagcctgt cccgcggaag
     3481 cgggacagcc cgaggggcgc acgaagtgcg gcaattgcgc caaccgcccc gcgtaaacgg
     3541 ggatgcgggg tccccgtttc cccgcaaaca aaggcgccca atggggagga gggtccccat
     3601 ttccccaccc ggcaagcgcc tctacaactc gttgggtatt tcggaatcga aattgcctca
     3661 gccttccccg aactgcggat gtccgaggat ggcctgttca aacgcggcgc gttcgtccgg
     3721 caaaacccac gcggggatgc gcgcgccgga gcggtgccat ttcttcagcc agtccgcgcc
     3781 gagcagttcg tggcgctggc cgaagaggcc gcttcgcgcc gccggcgtca gggcgatcgt
     3841 cgtctcctcc ttgtcgccgg tgctccggcc ccgcgcgacc cagaatcccg cggcgagcag
     3901 ggccgagcgg gcggcggtgg agatcgtgta ggtgaacaat tccgccgcct ttcccgagca
     3961 gctggcgaag agctgccgga aggccgccag cgtccaggcc tcgccgtggg tcttgctcga
     4021 aaacatgtcg taaaagatga cctccggcgc cgaatccgcc tgggcgagcg tttcgggaaa
     4081 gctgccgggg atcaaggtcc agctcaggcc gggatgcgtt ttggattgcc agtggccctg
     4141 tttcaggatg cccgccggcc cgccgtggcg cagatacggg aagcggtcgt tgtgcttgaa
     4201 ggccagccgg agcgaatcca ggtcgttttc aaagctgacg atccgcatcg ggcggaccgg
     4261 cccggcggcc gcctgcgcct cgtagcaaag aatggcgccc atggcattgg ccgccgcgcc
     4321 aaggccgacg tcccagacga ccaacggctc cgcgcccgcc tccggccgca cgcgttcggc
     4381 gaggcgggac tgctcgatgt aaagccgccg cgcctcgtcc atgggcggcg tgcgcgagtg
     4441 catgatctcg cccgaggaca cgtgcaggat gctcgcgaaa ccctcgcggg cggtgtggat
     4501 ttcgtaatcg cccagccggg gcttccggac gatccgcggc ttgacggcgg ttgtcgggtt
     4561 gtccaggtcg tcctcctgca ggaacgtccg cttggcgtgg tagagctcca ggaaccggtc
     4621 cgccagaatg ctcgcgcgaa tctcgcgcat gagctgatgg taaaaatgga gattgtgttt
     4681 gccgagcagc tgccagccga gcgtttcctt ggtcttggtc aggtggtgca gatacgcccg
     4741 tgaataccgc gagcaggtcg ggcagccgca gttcgggtcc aggggctcct cggaaaattt
     4801 gtaaaccgcg cggcgcagct gcaaataacc gcgggacgta aacacgccgc cgcgctgggc
     4861 gacctgcgtg ggaatgatgc agtcgaacat gtccacgccc cggtacaccg cttccagaat
     4921 gtccagcggc gtgccgacgc ccatgaggta acgcggcatt tcctccggga tcagcgcggc
     4981 ggagaactcg cacaaatcct cgcgttcgct cttggtttcc cccaccgcga ggcccccgat
     5041 cgccaggccg tcgaagggca tttccagcag cccctccgcg ctttggcggc gaagctcggg
     5101 aaacagcgcg ccctgaagga tgccgaacag cgcctgcggg gactcgccgc gcgcggccag
     5161 gcttcgcgcc gcccagcgct gggtgatttc caccgccgcc cgcgccgtgg cttcatccga
     5221 ggtcgagggc acgca
//
LOCUS       DATDAY010000409         2891 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_1034825, whole genome shotgun sequence.
ACCESSION   DATDAY010000409 DATDAY010000000
VERSION     DATDAY010000409.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2891)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2891)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2891
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1034825"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            122..1747
                     /locus_tag="VIS74_08060"
     CDS             122..1747
                     /locus_tag="VIS74_08060"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEY5793238.1"
                     /translation="MIEYLPALKEVARGQLDNWAKGAAVLHRYRKGETVCTEGEFGST
                     AYYIVSGTVDISINNPMAHLRTKPGWGFFGRSVARMKSFLVDDSHDKRADPHERKFIG
                     IDANIDLPMTKPIAQLGAGELFGEMTCRTFQPRSATVITREPCVMVEMLRVILDMLVG
                     NRQVSDASKATSKVKMPTFKGTSFKVEMEKKYRERSLETHLRSVPLFGSVGDEFITYL
                     KEKVELVSYNQNQVICKEGEDADAFYLIRSGMVRVSQNMPGGEMVRTYLSRGDYFGEI
                     GLLKSIKRIATCTALDAVDVVKIPANEFNTMLEKFPGVRAQLEPVAEARMAVTKARTA
                     PPGLGLDDYLNQGLFEAQNLLLIDLDNCTRCDACVQACAKAHDGVSRLLRDGLRYDHF
                     LVATACRSCRDPLCMTQCPVGSIRRKENLEIIIEDWCIGCGKCAELCPYGNINMHPLE
                     VMKEVKTEIKSATPGVPPKVKIEKKKITAYKANTCDLCTQLSVPSCVYACPHDAAKRV
                     DPQRFFGDALEAQAEILKGSWSPERRYAARTTH"
     gene            1791..>2891
                     /locus_tag="VIS74_08065"
     CDS             1791..>2891
                     /locus_tag="VIS74_08065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5793239.1"
                     /translation="MRIYNWKHFPWFIFVCLATVAAGLLYVGNFHPEWMPAGIRLPTA
                     LIQATTEHQTVGGTPLGLAFGAISLGIFVFAALLNLRKKIPLWRVGTVQRWLRAHIWL
                     TLLTIPLILFHSGFHLGGPMTTLLVFIYALVMVTGIYGLALQHLMPRLMKDRLPAETV
                     FEQIPHIRAQLCVAAEKMRDSFKPAPPKKPDAGAPAPSPAKAVTAGAAVMASTKGELS
                     TPVARAKSVTGSTPTAEPVAVTAAATAAAPVAKAATADPEPVILSAANAAASAAPAKP
                     AETIGTPTARVQSEPPGAPVPRASVPPGDEPAPNPPVVPASPAAAAPEKSATASPPPS
                     TPAPKPAESADPKPTAPAAAKPATPAAAKPAAA"
BASE COUNT          570 a          912 c          878 g          531 t
ORIGIN      
        1 atcagacggc gaccgttaaa aggcttccac cggaaagggc gcgttgagta tcatccctcg
       61 tagcgcgatg gacgaaccac tcgggaagct tacttcggga gagccgctta gcgtcgatca
      121 gttgatcgag tacttgccgg ccttgaagga ggttgcgcgg gggcagctcg acaactgggc
      181 gaaaggcgcc gccgtcctcc atcgttatcg caagggcgaa accgtctgca ccgaaggcga
      241 gttcggctcg accgcttatt acatcgtctc aggcacggtc gatatttcca ttaacaatcc
      301 gatggcgcat cttcggacga agcccggctg gggattcttc ggacggagcg tcgcgcgcat
      361 gaagagtttt ctggtggacg acagccacga caagcgcgcg gatccgcacg agcggaaatt
      421 tatcggcatc gacgcgaaca tcgatctgcc catgaccaaa ccgatcgcgc agctcggggc
      481 gggcgagctt ttcggcgaaa tgacctgccg caccttccag ccgcgctcgg ccacggtcat
      541 tacccgcgaa ccgtgcgtga tggtggaaat gctgcgcgtg atcctcgaca tgctcgtcgg
      601 caatcgccag gtctccgacg cctcgaaggc gacttcgaaa gtgaaaatgc ccacgttcaa
      661 agggacctcg ttcaaagtgg agatggaaaa aaaatatcgc gagcgctcgc tcgagactca
      721 tctgcgcagt gtcccgcttt ttgggagcgt cggagacgag tttatcacct atctcaaaga
      781 gaaggttgag ctggtctcct acaatcaaaa ccaggtcatc tgcaaggaag gcgaagacgc
      841 ggacgcgttt tatctgatcc ggtccgggat ggtccgcgtc tcgcagaaca tgccgggcgg
      901 cgaaatggtc cgcacttatt tgagccgagg cgattatttc ggcgagatcg ggttgctcaa
      961 gtcgattaag cgcatcgcca cctgtaccgc gctcgatgcg gtggatgtgg tcaaaatccc
     1021 cgcgaacgag ttcaacacca tgctcgaaaa atttcccgga gtgcgggcgc aactcgagcc
     1081 ggtggctgaa gcccgcatgg cggtgaccaa ggcacgaacc gcgccgcccg ggctcggcct
     1141 cgacgattat ctcaaccagg gattattcga agcgcagaac cttttgctca tcgacctgga
     1201 taattgcacg cgctgcgacg cctgcgtcca ggcctgcgcc aaggcgcacg acggcgtctc
     1261 gcgtttgctg cgcgacggct tgcgttacga tcatttcctg gtcgcgacgg cctgccgctc
     1321 ctgtcgcgat ccgctctgca tgacgcagtg cccggtcggc tccattcgcc ggaaggaaaa
     1381 cctcgagatc atcatcgagg attggtgcat cgggtgcggg aaatgcgccg agctctgccc
     1441 ctacggcaat atcaacatgc acccgctcga ggtgatgaaa gaggtgaaga ccgagatcaa
     1501 atcagccact ccgggtgtgc cgccgaaggt gaagatcgag aagaaaaaga taaccgccta
     1561 caaggcgaac acgtgcgatc tctgcacgca gctctcggtc ccgagctgcg tttatgcctg
     1621 cccgcatgac gcggcgaagc gggtcgatcc ccagcgattt ttcggcgatg ccctggaggc
     1681 gcaggcggag attttaaagg gaagttggtc gccggagcgg cgctacgcgg cgcgcaccac
     1741 gcactagata attttcgaat ttcgaagttc gagtttcgag ttttcacggc atgaggatct
     1801 ataactggaa gcacttcccc tggtttatct ttgtctgcct ggccacggtc gcggctgggc
     1861 ttctctacgt gggcaacttc catccggagt ggatgccggc ggggattcgg ttgcccactg
     1921 ccttgatcca ggccacgacc gaacatcaga ccgtgggcgg cacgccgctc gggctggcct
     1981 ttggcgcgat ctcgcttggg atttttgttt tcgccgcgct cctcaatctc cggaaaaaaa
     2041 tacctctgtg gagagtagga accgtgcagc gatggttgcg cgcccatatc tggctcaccc
     2101 tgctcacgat tccgctcatt ctctttcaca gcggttttca cctgggcggg ccgatgacga
     2161 cactgctcgt gttcatctat gcccttgtca tggtgaccgg gatctacggg ctcgcgctcc
     2221 agcatctgat gccgcgtttg atgaaggacc ggctgccggc cgagaccgtt ttcgagcaaa
     2281 ttccgcacat ccgcgcccag ctctgcgtcg ccgcggaaaa aatgcgcgac tcgttcaaac
     2341 ctgctccgcc caagaagccg gatgcgggcg cgcccgcgcc gagtcccgcg aaggcggtga
     2401 cggccggcgc cgcggtgatg gcttccacga aaggcgaact aagcactccg gtggcgcgcg
     2461 ccaagtcggt gactggctca accccaacag cggagcccgt ggccgttacc gcggcggcca
     2521 ccgccgcggc gccagtggcc aaggcggcga cggccgatcc cgaaccggtg attctttccg
     2581 cggccaatgc tgccgcatcg gcggcgccgg cgaaacctgc ggaaacgatc ggcactccca
     2641 cggcgcgagt gcaaagcgaa ccgccgggcg ctcccgttcc gcgcgcatcg gttccgccgg
     2701 gcgacgaacc ggcgccgaat ccacctgtgg tcccagcgtc gcccgcagcc gccgctccgg
     2761 agaaatccgc gacggcttcc ccgccgccat caacccctgc gccgaaaccg gctgagtcag
     2821 cggacccgaa accaaccgcg ccggccgccg ccaagcccgc gacacccgcc gcggcgaaac
     2881 ccgctgccgc g
//
LOCUS       DATDAY010000410         3339 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_2096186, whole genome shotgun sequence.
ACCESSION   DATDAY010000410 DATDAY010000000
VERSION     DATDAY010000410.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 3339)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3339)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3339
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2096186"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..936)
                     /gene="argS"
                     /locus_tag="VIS74_08070"
     CDS             complement(<1..936)
                     /gene="argS"
                     /locus_tag="VIS74_08070"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF012952.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEY5793240.1"
                     /translation="MSQKTLRTELTARLHEALREAGLPAENAEVVPAADTRFGDYQTN
                     AAMTLAKSRKANPRQLAQAILEKLAVSDLSDPPTLAGAGFINFSLSLPALAGRVAALA
                     RDERLGVPPAAKPETIVIDFSSPNVAKPMHVGHIRSTLIGDCLARVARALGHKVITDN
                     HLGDWGTQFGKVIYGWKHLLNPEALAKDAIAELVRLYRDVNALEETDPEIKRRVREEL
                     VKLQRGDAENRAIWQQTVDLSWQEFDRLYALLEIEFDERLGESFYNDALEPLVKRLRE
                     SGLAEVSEGALCIFFRDLPALADKPCLVRKSDGGFN"
     gene            complement(1031..1495)
                     /locus_tag="VIS74_08075"
     CDS             complement(1031..1495)
                     /locus_tag="VIS74_08075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEY5793241.1"
                     /translation="MTLAKLLSVEQILPEMQAAERWQAITEIVDNLIALRRIRETDRE
                     TVLAALRQREETMSTGIGFGIAIPHASSDCVSTVVAGFGRSKAGIEFDSLDNKLVHFI
                     VLFVVPRDQFQTHLRTLAAIAKFLNDPAVREELANAGDAAAILRVFENRAPR"
     gene            complement(1513..2505)
                     /locus_tag="VIS74_08080"
     CDS             complement(1513..2505)
                     /locus_tag="VIS74_08080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5793242.1"
                     /translation="MNFPRLAAASSDAVMCGLMNLLQWRLRREVCTRDELLAYLDRCA
                     AQTREEFYALPLAGEFEVAGRRVRWPSPVKSGFVENDSACAEVFLLPERPEAPTVLIL
                     HALMSASANGYRKLAAWFNARGWQAAILHLPFHYSRVPRGYFNGSQAISANLIRNGET
                     LRQGVVEVRQLMEWFRARGCRGFGLIGTSFGGWIAALTSFLERDFHFVSLIQPIVNVD
                     HALWHNPSSRSMKALLRKQGIGPEDARRHEHLSSPLHGRPLCGGERVLICAGRYDTVS
                     PSAELKKLADLWTGAELREVEQGHFGHAALRETVRVIESKMARGIFVAFPNKSA"
     gene            complement(2502..3197)
                     /locus_tag="VIS74_08085"
     CDS             complement(2502..3197)
                     /locus_tag="VIS74_08085"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEY5793243.1"
                     /translation="MPSDTAYHFWNTIAKGLLVGRVRVRVLHPERGAAPGAWILASNH
                     ISHFDPTLLSTVTRRAVDWMAMEELFRGRLAGAFYRGIGAFPVKRSRADRTALRTAIQ
                     RLEAGRVVGIFPEGGLRAGPASVLEGAPAKSGIALLSVLAQVPIQPCVVIGTDRLYRA
                     KNWRPGPRIPVWMAFGERIQPDPALEKTEAMAKVERELAAAFLALKGQLIGEFGLTTN
                     DLPQTPQHRKGEA"
BASE COUNT          617 a         1103 c         1043 g          576 t
ORIGIN      
        1 gttgaatccg ccgtcgctct tgcgcacgag gcagggtttg tccgcgagcg cgggaaggtc
       61 gcggaaaaaa atgcagaggg cgccttcgct gacttccgcc aggccggatt cccgcaggcg
      121 cttcacgagc ggctcgagcg cgtcgttgta aaaactctcg cccaggcgct cgtcgaattc
      181 gatctccagc agcgcgtaga gccggtcgaa ttcctgccag gaaaggtcca cggtctgctg
      241 ccagatggcg cgattttccg cgtcaccccg ctgcaatttg accagctcct cgcgcacccg
      301 gcgcttgatc tcgggatcgg tttcctccag cgcgttcacg tcgcggtaaa gccggacgag
      361 ttcggcgatt gcgtccttcg ccagggcttc ggggttgagc agatgtttcc agccgtaaat
      421 caccttgccg aactgcgtgc cccagtcgcc gaggtggttg tcggtaatga ccttgtgccc
      481 gagcgcccgc gccacgcgcg ccaggcagtc gccgatcagc gtactgcgga tgtgcccgac
      541 gtgcatcggc ttcgcgacat tcggcgagct gaaatcaatg acgatcgtct ccggcttcgc
      601 ggccggcggc acgccgaggc gttcgtcccg cgcgagcgcg gcgacgcggc cggccagcgc
      661 ggggagcgag agggaaaaat tgatgaatcc cgcgcccgcg agcgtcggcg gatcgctcag
      721 gtcggaaacc gccagttttt ccagaatggc ctgcgcgagc tggcggggat tggccttccg
      781 cgacttcgcc agcgtcatgg cggcgttggt ctgataatcg ccgaaacgcg tatccgcggc
      841 cggaaccact tccgcgtttt ccgccggaag cccggcctcg cgcaacgctt catgaaggcg
      901 ggcggtcagc tcggtccgca gggttttttg agacattgat taaaaggatt ggaccacaga
      961 gagcaccgag gacacagaga taaaatcaat taaatcccag cttcttcctc ggtgtcctct
     1021 gtggttgaaa ttaccgcggc gcgcggtttt cgaacactcg caggatggcc gccgcgtccc
     1081 cggcgttggc gagctcctcg cggacggcgg gatcattgag aaatttcgca atcgccgcca
     1141 gcgtgcgcag gtgggtctga aattggtccc gcggcacgac gaagaggacg atgaaatgca
     1201 ccaatttgtt gtcgagcgaa tcgaattcga tgcccgcctt cgaccggcca aaccccgcca
     1261 ccaccgtgga aacgcagtcc gacgacgcgt gcgggatcgc gatgccaaag ccgatgcccg
     1321 tgctcatggt ctcctcgcgc tggcgcaggg cggccagcac cgtttcccgg tccgtctcac
     1381 ggatgcgccg cagagcgatg aggttatcca cgatctccgt gatcgcctgc caccgctctg
     1441 ccgcctgcat ttcgggaagg atctgttcga ctgagagcag cttggccaga gtcatagggc
     1501 agatttaagt cattaagcgg atttattcgg gaaggctaca aaaattcccc gcgccatttt
     1561 ggattcgatc acccggacgg tttcgcggag cgcggcgtgg ccgaagtggc cctgctcgac
     1621 ctcccgcagc tccgcgccgg tccataaatc cgccagtttt ttcaattccg cggaggggga
     1681 aaccgtgtcg tagcgcccgg cgcaaatcag cacgcgctcc ccgccgcaga gcggccggcc
     1741 atgcagcggc gagctcaggt gctcgtgccg ccgggcatcc tccgggccga tgccctgctt
     1801 ccgcagcaac gccttcatgg agcgcgacga gggattatgc cacagcgcgt ggtccacgtt
     1861 cacgatcggc tggatgaggc tgacaaaatg aaaatcgcgc tccagaaacg aagtcagcgc
     1921 cgcgatccag ccgccgaagc tggtgccgat caggccaaac ccccggcagc cgcgcgcgcg
     1981 gaaccattcc atcaattgcc ggacctcgac gacgccctgg cggagcgttt cgccattgcg
     2041 aatgagattc gcggaaatcg cctgggagcc attaaaatac ccgcgcggca cgcggctgta
     2101 gtgaaagggc aggtgcagga tcgccgcctg ccagccgcgg gcgttgaacc acgcggccag
     2161 cttgcggtag ccgttcgcgc tggcggacat cagcgcgtgc aggatcagca cggtcggcgc
     2221 ctccggccgt tccggcagca aaaacacctc ggcgcaggcg gaatcatttt ccacaaagcc
     2281 gctcttcacc ggcgacggcc agcgcacccg ccggccggcc acctcaaact cacccgcgag
     2341 gggcagcgcg tagaattcct cgcgcgtctg cgccgcgcac cggtccagat acgcgagcag
     2401 ctcgtcccgc gtgcaaacct cgcggcggag ccgccattgc agcagattca tcaggccgca
     2461 catgacggca tccgacgacg cggcggcgag gcgcgggaaa ttcatgcctc ccccttccgg
     2521 tgctgcggcg tctggggcaa gtcgttggtc gtcagaccaa attcaccgat caattgtccc
     2581 ttgagcgcga gaaacgcggc ggcgagttcg cgttcgacct tggccattgc ctccgttttc
     2641 tccaacgccg gatcgggctg gatgcgttcg ccaaaggcca tccagactgg aatgcgcgga
     2701 cccggccgcc agtttttggc gcggtagagg cggtcggtcc cgatcaccac acaaggctga
     2761 atcggaacct gggccagcac gctcagcagc gcgatgcccg acttcgccgg cgcgccctcc
     2821 agcacggaag cgggtccggc ccgcagcccg ccctccggaa aaatgcccac cacccggcca
     2881 gcttcgaggc gctggatcgc cgtccgcagc gcggtccggt ccgcccggct gcgcttcacc
     2941 ggaaacgcgc cgatgccgcg ataaaacgcc ccggccagcc gcccgcgaaa caactcctcc
     3001 atggccatcc aatccaccgc gcgccgggtg accgtgctga gcagggtcgg gtcgaagtgg
     3061 ctgatgtgat tgctcgccag aatccaggcg cccggcgcgg cgccgcgctc gggatgcaaa
     3121 acccgcacgc gcacgcggcc gaccagcagc cctttggcga ttgtgttcca gaaatgatag
     3181 gcggtatccg aaggcacgaa atccgcgatc agggtttgga aaaactcatt ttggcaaaaa
     3241 gaatccgcgc tgtttagcgg aaatcggcca gccgagcttt tccatggcca gcatcatgtc
     3301 ctcccacacc ttgcgctttt cggaaagggt ctggttccg
//
LOCUS       DATDAY010000411         3554 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_1919376, whole genome shotgun sequence.
ACCESSION   DATDAY010000411 DATDAY010000000
VERSION     DATDAY010000411.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 3554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3554
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1919376"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..558)
                     /locus_tag="VIS74_08090"
     CDS             complement(<1..558)
                     /locus_tag="VIS74_08090"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HEY5793244.1"
                     /translation="MPAKPSKSAAANFGFFRVGVASPEHRVADCAFNAAEIVRVLGQA
                     RTDGCQLVVFPELAVTGYSCADLFYQPQLLAAARESVAAIAKACGAKRIGAVVGAPLE
                     VEGRLFNAAIVIGADGWIAGVVPKTYLPTTGEFYEARWFTRGDAAAPGVIDWNGREAP
                     FGVDLLFPAGEALVGVEICEDLWAIK"
     gene            792..1829
                     /locus_tag="VIS74_08095"
     CDS             792..1829
                     /locus_tag="VIS74_08095"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HEY5793245.1"
                     /translation="MTFEPPPVPEPVPCPKQAPSLFSHLLTVLQVAVTLVLLWWLFHD
                     AERRQVMSEALKMADWRWMGLALAAAGVCEFFGILRWQLFLRMLHIKVSFREITRLFF
                     IGAFFNQFLPGTTGGDIVRVIFLMKDHPENKTEGLLSVAVDRLLAVFVLVIMGLLFAW
                     SRSAWFAQSFAVGNLMKWFAIALFVMAVALIGSFVLTSRHLVTRLPRKIPFRKSIIKL
                     SRLWQLCLENRREAALGALYTVPMLLAYFAAFAFAAKAFTHKVSFLDMTSIMPLITAI
                     SSLPISLNGIGVREALFEELLAELCGVSRGTGLLISIAGMTVYLIWGLVGGVFYLERI
                     KRRYRFAGEKP"
     gene            1916..2455
                     /locus_tag="VIS74_08100"
     CDS             1916..2455
                     /locus_tag="VIS74_08100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5793246.1"
                     /translation="MNNSTTNPFLGQTSSSPIYFLETLWMGMDHIAAELRSIETKKLA
                     QEKIRCPGLLATSSSYDAEDRFIANYFIWYANSAVNFLDLFHVTFRPSIYYMAEFSAL
                     CKWRNKVAAHTAYAKPKGENTVTKNYSLSALLPEYMGDRYGVASWVLQDASGSTPDDW
                     SWYLTTEHERIFKYVIGAL"
     gene            complement(2707..>3554)
                     /locus_tag="VIS74_08105"
     CDS             complement(2707..>3554)
                     /locus_tag="VIS74_08105"
                     /inference="COORDINATES: protein motif:HMM:NF019344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY5793247.1"
                     /translation="IGSFVFLGPTGVGKTFLARTLAEFMFGDADALIQLDMSEYMEKF
                     TASRLIGSPPGYVGYEEGGQLSEAVRRRPYSVVLFDEIEKAHPDVMHLLLQILEEGKV
                     TDSLSRKIDFRNTIIIMTSNVGAELIKKQTTLGFGAPKLDDNYDAMREKILDETKRVF
                     KPEFLNRLDDIIVFHTLTKPNLIKIVDLEVSKVIERIRSKHVYVELDQTAEDFLIEKG
                     YDPQYGARPMRRAVEKYVEDPLAEELLRGNVKAGDKVLVKQNGDKLIFETTNGAQPEP
                     APASK"
BASE COUNT          714 a         1028 c         1001 g          811 t
ORIGIN      
        1 tttgatggcc cagagatcct cgcagatttc gacgccgacc agcgcctcgc cggccgggaa
       61 tagcaggtcc acgccgaagg gcgcctcccg cccattccaa tcaatgacgc ccggcgccgc
      121 cgcatcgccg cgcgtgaacc agcgggcttc gtaaaattcg ccggtggtgg gcaggtaggt
      181 cttcggcacc acgccggcga tccagccatc cgccccgatc acgatggcgg cattaaacag
      241 gcgcccctcg acttccagcg gcgcgcccac cacggcgccg atgcgcttcg cgccgcaagc
      301 cttcgcgatg gcggccacgc tctcccgggc cgcggccagc agttggggct gatagaacaa
      361 atccgcgcag gagtagccgg tgacggccag ctccggaaac accaccagct ggcagccatc
      421 cgtccgcgcc tggccgagca cccggacgat ctccgcggca ttgaaggcgc aatccgccac
      481 gcgatgttcc gggctcgcga cgccgacgcg gaaaaaaccg aagttcgccg cggcggattt
      541 ggatggtttg gcaggcatgg aaaaaatcta gcgtaaatcg ttcaggagca cacgggaatc
      601 ctgcaatccg ggcaaactga accacggatg gacccggata aacacggatt tatttggaca
      661 tccggaaatc ggacaagctt ccggtttttc tcatctgtgt tcatccgggt ccatccgtgg
      721 ttcagttttg aacggcgcct tcatctccgc gattgcaacc tcaatccctt cttgcattat
      781 ctcgtgcgtt gatgactttt gagcccccgc ccgtgccgga acccgttccc tgtcccaagc
      841 aggctccgtc gcttttcagc cacctgctga ccgtgcttca agtggcggtc acactcgtcc
      901 tgctttggtg gctttttcac gatgcggaac gccgccaggt gatgagcgag gctctgaaaa
      961 tggcggactg gcgctggatg gggctggcct tggcggcggc gggcgtttgc gaattcttcg
     1021 gcatcctgcg ctggcagctt tttctccgga tgctccatat caaggtgtct ttccgggaaa
     1081 tcacccggct gtttttcatc ggggcattct ttaatcaatt cctgcccgga acgaccgggg
     1141 gggatatcgt ccgcgtgatc tttctcatga aggatcatcc ggagaacaaa acggaggggc
     1201 ttctcagcgt ggcggtcgat cggctgctgg cggtcttcgt cctggtgatc atggggctgc
     1261 ttttcgcctg gagccgcagc gcgtggttcg cgcagagctt tgccgtggga aacctcatga
     1321 aatggttcgc gatcgcgctc tttgtcatgg cggtggcgct catcggatcc ttcgtgctga
     1381 cgagccggca tttggtcacc cgccttccgc ggaaaatccc ctttcgaaaa tcgatcatca
     1441 aactctcgcg gctttggcag ttgtgcctgg aaaaccgccg cgaggcggcg ctgggcgccc
     1501 tttacacggt gccgatgctt ttggcctact ttgcggcctt tgccttcgcg gcgaaggcgt
     1561 tcacccacaa ggtcagcttt ctggacatga cctcgatcat gccgctgatc accgcgattt
     1621 cgtcgctgcc catcagcctt aacggcatcg gcgtgcgcga ggcgctcttc gaggaattgc
     1681 tcgccgagct ttgcggcgtc tcgcgcggaa ccgggctgct catttccatc gcgggcatga
     1741 cggtttatct gatctggggg ctggtgggag gggtctttta cctcgagcga atcaagcgcc
     1801 gctaccgttt cgccggtgaa aaaccgtaag tcgggaagag ccgcgaaagc cgctgcccat
     1861 agtaaaaatt aaaaatgcgc gcagtctaac ggcgcgtttg attttcttca aaccaatgaa
     1921 caactccaca actaatccgt ttctcgggca aaccagctcc agcccgatct attttttgga
     1981 aacgctttgg atgggaatgg accacatagc cgccgagcta cgctcaattg aaacaaaaaa
     2041 gcttgctcaa gagaaaatcc ggtgtccggg acttttagca acatccagct cctatgatgc
     2101 cgaagatcgt tttattgcaa actatttcat ctggtatgca aactcagccg taaatttcct
     2161 cgatttattt cacgtcacat ttcggccttc aatttattat atggcagaat ttagcgctct
     2221 ttgtaagtgg cgcaacaaag ttgctgcgca tacagcatat gcgaaaccca agggtgaaaa
     2281 tactgtcacc aaaaattata gcctttctgc attattgcca gaatatatgg gtgacagata
     2341 tggagttgca tcatgggtgc ttcaagatgc atctggctcg actcctgatg attggtcttg
     2401 gtatttgacg acagaacacg aacggatttt caaatacgtt ataggagctc tttagttcta
     2461 gagcagttgc cgaaataaat ttccggaaac ttggagggaa acgctttgtc gtttcccttg
     2521 aattcaaagg cactgacgaa gcagtgccct ccatttcgga aacgaattct ggcatctgct
     2581 ctaaatatga caaaagggcg acagcatcac tccatgacag atctctgatg cgaaccccgg
     2641 acggtttcac cgcccgccta gcgcaactgc ggaaagcgga acggttgccg tccttaatcc
     2701 cgcgggttac ttggaagccg gcgccggctc gggttgcgcg ccgttcgtgg tttcgaaaat
     2761 gagcttgtcg ccattttgtt tcaccagaac tttgtcgccg gccttcacgt tgccgcgcag
     2821 caattcttcc gcgagcggat cctcgacgta tttctccacg gcccggcgca tcggacgcgc
     2881 gccgtattgc gggtcgtagc ctttttcgat gaggaaatcc tccgcggtct ggtccagctc
     2941 cacatacacg tgcttgctgc gaatgcgttc gatcaccttc gacacctcga gatccacgat
     3001 cttgatgaga ttgggcttcg tgagcgtgtg gaagacgatg atgtcgtcga ggcggttcaa
     3061 gaactcgggc ttgaagacgc gtttggtctc gtcgaggatt ttttcccgca tcgcatcgta
     3121 gttgtcatcg agcttcggcg cgccaaaacc cagcgtggtc tgcttcttga tcagctcggc
     3181 gccgacgttc gacgtcatga tgatgatcgt attccggaaa tcgatcttgc ggctcaggct
     3241 gtcggtcacc ttgccttcct ccaggatctg gagcagcagg tgcatcacat ccggatgggc
     3301 tttctcgatc tcgtcgaaga ggacgacgga atacggccgg cggcggaccg cctcggagag
     3361 ctggccgccc tcctcgtaac cgacgtaacc cggaggcgag ccgatgaggc gggaggcggt
     3421 gaacttctcc atgtattccg acatgtcgag ctggatgagc gcatcggcgt cgccgaacat
     3481 gaattcagcc agcgtgcggg cgaggaaggt cttgcccacg ccagtcgggc cgaggaacac
     3541 gaacgacccg atcg
//
LOCUS       DATDAY010000412         7992 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_2096823, whole genome shotgun sequence.
ACCESSION   DATDAY010000412 DATDAY010000000
VERSION     DATDAY010000412.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 7992)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7992)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7992
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2096823"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..593)
                     /locus_tag="VIS74_08110"
     CDS             complement(<1..593)
                     /locus_tag="VIS74_08110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HEY5793248.1"
                     /translation="MALEFTDPWMLALLPLGWFLWSWQHRSPAPLPPRRRRWSTGLRL
                     TIFALMVLALTDPRWRSSSRQSHYLWMADVSRSVDEKALDRAREIREKIGAAPAGSSE
                     SWLLFAGRTAVAADAAAARKIVRKELNDEETNLAGALQFAMASFPVDRGKTALLFTDG
                     VETVGRASDQLRALREAGVRVVSIPIVPPDKPEILVKA"
     gene            complement(597..2258)
                     /locus_tag="VIS74_08115"
     CDS             complement(597..2258)
                     /locus_tag="VIS74_08115"
                     /inference="COORDINATES: protein motif:HMM:NF024909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatA and WFA domain-containing protein"
                     /protein_id="HEY5793249.1"
                     /translation="MSFLLPLFWSALALAIPVTIFYLIREQPRRRLISSLLFWQHADS
                     KVSEAPLWRKLRRWISLLLQLLFLALLVFGLTRPMAKWESLQARARIFILDGSASMRA
                     TDIAPNRFEAARSAIRREIRQMRFFDESAVLLAGENPEILSGWTRSKRRLLEALDRAG
                     PATKAADIPAASNLAADLIAARKSPRIEWFSGGVVGTEELPKQKEAAWQWFAGRPENM
                     GLTQLAARRSFVSPGEFTLGVRADAGGESPVDGEIEIFRNGALLDAQSVTLTPGKSWE
                     RSWNFTARDGGEFEARLVKKGTDHLEQDNSGKLTVSPLEEISVTLVAPDDSFLAAAFY
                     SMPGVQLARVWPPEKAGSGDPGKVWVFSGATPPENFQARAMILLAPEQGGFWGNYRGE
                     LNSVLVSEVNETSPLMRFVSLSPVQAGRALDFQPAAGAEVEAASFGHPLIFGKWKEPF
                     PWLVIGFDLEKSDLVLRAAFPILLANAVQSLRISQTPLIEAEFPGISQTRLVSAKSPK
                     VEREAKNLPGWTNVIGSHPLWWWALLLGIGWIFAEWWAYHRRITE"
     gene            complement(2269..3159)
                     /locus_tag="VIS74_08120"
     CDS             complement(2269..3159)
                     /locus_tag="VIS74_08120"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEY5793250.1"
                     /translation="MVITDPDELFDAGLLQRYESLSLLARKLVRGRMRAERRSVRRGA
                     SVEFAEYRPFVAGDDYRYIDWHAFARWRQLVLKLFIEEEDLHVHLLLDCSQSMDWGAP
                     VKFDQARRLIAGLAYIALANLDRASIVPLGLEAPPGLPPSRGRERFLQIIRYLARCPV
                     SSGVSRLEDRARAWLASRPRRGSVVWVSDLLGRDPEDALRALDRLRHARHEVAIIHLL
                     DPSETLAGDPGEYEFEDCESGQVRRVVVDQAAARAFRQRVADYRESLSRYGKKHQIPI
                     LSAQTTDEVAGLLGLALRWQ"
     gene            complement(3190..4185)
                     /locus_tag="VIS74_08125"
     CDS             complement(3190..4185)
                     /locus_tag="VIS74_08125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEY5793251.1"
                     /translation="MNPDASADAVEFRNKFVQLKTEIQKVIVGQDEIIENVLIAIIGG
                     GHVLLEGVPGLGKTMLVRSIANALKLSFQRVQFTPDLMPADLIGTNILVDDGHGHSRF
                     EFSRGPLFCNLLLGDEINRATPKTQSALLEAMQEHHVTVAGETRPLGPPFFVLATQNP
                     LEQEGTYPLPEAQLDRFFFKLKVPYPRMEEFEEILRRTTEKTHTDVKALFGGHELIAM
                     GELARAVPVAETLRRRAVELVMATHPETAEASGEIKRFVRYGASPRAGQALILAAKIR
                     ALLRGRFHVAVEDILDSMLPVLRHRVMLNFEAQADGVDSDALMERLRNAKRKAWA"
     gene            complement(4193..5251)
                     /locus_tag="VIS74_08130"
     CDS             complement(4193..5251)
                     /locus_tag="VIS74_08130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5793252.1"
                     /translation="MIGLFLPVTNASRAMVPEVALSGEQAADLEDLFKDWEQAEQKDS
                     DPVLEQMLRELEPFRKQLAEGKATPRETFLELSKLEDKIAQVQQKLAAQSLDSFAADL
                     SEALGQAGGMEALAKALKERDYEGAEKAARELEAKQAADTASLPPALQPAETQEKLSK
                     LGQKLSQAGQSAMGQAVQSLSQAGKNSSSSDFKDGLKKMCQSLGQQNALGQQCKKLAL
                     QCNQLGMCKSCLGNGESMCQGMSLLPKLSLVKSQNPGKGAGSATDPNRQILPTDQMPA
                     GVHEQVSGTSNESGESETSIVSSEEAPRQQTVSVSTANLDAYRKLSEEAVEDESLPLA
                     HRQTIRRYFEMIRGQPAP"
     gene            complement(5784..6047)
                     /gene="rpsT"
                     /locus_tag="VIS74_08135"
     CDS             complement(5784..6047)
                     /gene="rpsT"
                     /locus_tag="VIS74_08135"
                     /inference="COORDINATES: protein motif:HMM:TIGR00029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEY5793253.1"
                     /translation="MANTKSAAKYALTSKRRHDQNAGIITGIKTEQKKIRAALASGDK
                     AAAQAGLKSVSAALDKAAKRGVIHANAADRRKSRFNKLVAKDS"
     gene            complement(6088..6162)
                     /locus_tag="VIS74_08140"
     tRNA            complement(6088..6162)
                     /locus_tag="VIS74_08140"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6128..6130),aa:Met,seq:cat)
     gene            6354..6998
                     /locus_tag="VIS74_08145"
     CDS             6354..6998
                     /locus_tag="VIS74_08145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5793254.1"
                     /translation="MVEVQIHGFSFERWVRGTLFEGYSGNYMQKWDIPPDFNQHESIP
                     EQFRNIPVSVKSAKYGSPIGLGDILRQRVIYQPFLMIAGFWRQRTPSEKWIEDIGAAF
                     FDPAQWNGLWGGLTSEGIQVIDAQIKDLGTPYTVARELAQQWKRRLADTSGSAVVVNP
                     KIDSKRQRRIQCSLPFRTFWEAAGRKPTVCESPALFGFEFENPILSGARTFNQD"
     gene            complement(6985..>7992)
                     /locus_tag="VIS74_08150"
     CDS             complement(6985..>7992)
                     /locus_tag="VIS74_08150"
                     /inference="COORDINATES: protein motif:HMM:NF013704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HEY5793255.1"
                     /translation="RVRKLIKDGRIPAEKRGRDHLIQEADLEWFAHHGRKNIGRPAEK
                     GFVSAIIIEDERMYHPSSKQIVSVGNGEIHIGDALNVLHTLEANQFQTIIADPPYFQV
                     LLEEKWDNTWQSSDAYLDWTLNWVRECKRVLCQDGLLYIFGQLGKREHVWLHTCSMLA
                     KEMQFHDMIIWDRAVGYNERYDSFTPQYEMILVLRHAANSKPHFDKGAVRLPYDEDKI
                     QSYLRDKRYKDKEARERHLRNGKYATNILRVPSLKGSSKEKIGHPSQKPIALINQLVS
                     SSSRKGDWVLDPFLGSGTSAASAEELGRRWVGIEVNPEYVAIAENRIKKILSVPEFNL
                     D"
BASE COUNT         1765 a         2419 c         2100 g         1708 t
ORIGIN      
        1 gctttgacca ggatttccgg cttatcggga ggaacaatcg gaatgctcac cacccgcact
       61 cccgcttccc gcagcgcgcg cagttgatcg gatgcgcgtc cgacggtctc cactccatcc
      121 gtaaaaagca acgcggtttt gcctcgatcg accgggaatg aagccatcgc aaattgcaac
      181 gctcccgcga ggttggtttc ctcatcgttc aactccttgc gcacgatctt ccgcgccgcg
      241 gccgcatccg ccgccacggc agttcttccc gcaaacaata gccagctttc gctgcttcca
      301 gctggagccg ccccgatttt ttcccgaatt tcgcgagccc ggtccagagc cttttcatcc
      361 acgctgcggc tgacatccgc catccagaga taatgggact gccggctgct gctccgccag
      421 cggggatccg tcagcgcaag caccatcaac gcaaatatcg tcaaacgcag ccccgtgctc
      481 caccgccgcc gccggggcgg caagggcgcc ggcgaacgat gctgccagct ccacaagaac
      541 cagccgagcg gcagcagcgc cagcatccat ggatcggtga attcaagcgc catggttcat
      601 tccgtgattc ggcggtgata cgcccaccat tccgcaaaga tccagccaat gcccaagagc
      661 agcgcccacc accacagggg atgagagccg atcacattcg tccagcccgg caggtttttt
      721 gcttcccgct caaccttggg cgatttggcc gaaaccagcc gggtttgcga aatcccgggg
      781 aactccgctt cgatcaacgg ggtttgcgaa attcggaggc tctgcaccgc gttcgccagc
      841 agaatcggga aagccgctcg caggacgaga tcgcttttct ccaaatcaaa cccaatcacc
      901 agccacggaa atggctcctt ccattttcca aaaatcaagg gatgcccaaa actcgccgct
      961 tccacctccg ctccagccgc cggttgaaaa tccaaggcgc gtcccgcctg aaccggagag
     1021 agcgacacaa accgcatcaa tggcgaggtt tcattcacct cgctcaccaa tactgaattc
     1081 agctcgcccc gatagttgcc ccaaaatccc ccttgctccg gagccagcag aatcatcgcc
     1141 cgggcttgaa aattttcggg cggcgtggct ccggagaaaa cccaaacctt tcccggatcg
     1201 cccgagcccg ctttttccgg cggccatacc cgggcaagct ggactccggg catggaataa
     1261 aatgccgccg caagaaagga atcgtccgga gcaacgaggg tcacggatat ttcctccagc
     1321 ggggataccg tgagcttgcc ggaattgtcc tgctccaaat gatccgttcc ctttttcacc
     1381 aggcgggcct cgaattcgcc gccatcccgg gccgtaaaat tccaggatcg ctcccaggat
     1441 tttccgggcg tcaacgtcac cgactgcgca tccagcaggg cgccattgcg gaaaatttcg
     1501 atctcgccat ccaccggcga ttcacccccc gcatccgcgc ggactcccag cgtaaattcg
     1561 cccggggaga caaaggaacg cctggccgcc agctgcgtca gtcccatgtt ttccgggcgg
     1621 ccggcgaacc attgccaggc cgcctctttt tgcttcggca gttcctccgt gcccaccacg
     1681 ccgccggaaa accattcgat tcggggtgac tttcttgcgg cgatcaaatc cgcggccaga
     1741 ttcgaagccg ccggaatatc cgcggccttg gtcgcaggcc cggcccgatc caaagcctcg
     1801 agcaaacgtc gtttgctgcg agtccatccc gaaaggattt ccggattttc gcccgcgagc
     1861 agcactgcgg attcgtcaaa gaaccgcatt tgccgaatct cccgacggat ggcgcttctg
     1921 gctgcttcaa agcggttggg agcgatgtcc gtggcccgca tgctggcgga accgtccaga
     1981 ataaaaatcc gggcccgcgc ctggagcgac tcccatttcg ccatcggccg cgtcagaccg
     2041 aaaaccagca atgcaagaaa cagcaattgc aacaacagtg aaatccagcg gcgcagcttc
     2101 cgccacagag gcgcttcaga tactttggaa tccgcgtgct gccaaaataa cagcgaactg
     2161 atcaaccgcc ggcgcggttg ctcacggatc aggtaaaaaa tcgtaacggg aatcgccagg
     2221 gccagcgcgc tccaaaacag gggaaggaga aagctcattt cgtccccttc attgccacct
     2281 caatgccagg cccaaaaggc cggccacttc atccgtggtc tgcgcgctta atatgggaat
     2341 ctgatgtttt ttcccataac gggaaagcga ttctcgataa tccgcaacgc gctgacggaa
     2401 ggcgcgagcc gccgcttgat ccaccaccac ccgcctgacc tggccgctct cgcagtcctc
     2461 aaactcgtat tcccccggat cgccggccag ggtttccgac ggatccagca agtgaatgat
     2521 cgccacctcg tgccgggcgt ggcgcaaccg atccagcgcc cgcaaggcat cctccggatc
     2581 gcggccaagc aggtcactca cccaaaccac cgaaccgcgc cgcggacggc tcgccagcca
     2641 ggcgcgggcg cgatcttcca gccggctgac gccggacgaa accgggcagc gcgcgagata
     2701 tcgaatgatc tgcaaaaaac gctcccgtcc acgcgacggc ggcaagcccg gcggagcttc
     2761 caagcccagc ggaacgatcg aggcacggtc aagatttgcc agggcaatat aagccagccc
     2821 ggcaatcagg cggcgggcct ggtcaaattt caccggtgct ccccaatcca tggattggct
     2881 gcagtccagc aacaaatgga cgtgcaggtc ttcctcctca ataaataact tgagaaccaa
     2941 ttgacgccag cgcgcgaagg catgccaatc gatgtagcga taatcgtccc cggcgacaaa
     3001 agggcgatat tccgcaaatt ccacgctcgc tccgcgccgc acgctacgcc gttccgcccg
     3061 catgcggccg cgcacgagct ttcgtgccag cagacttaac gactcatagc gctggagcag
     3121 cccggcatca aaaagctcgt ctggatcggt gatgaccacg gcgatttgga tcggcccttt
     3181 ccgacaccct taagcccacg ccttgcgctt ggcattccgg agccgctcca tcagcgcatc
     3241 cgaatccact ccatccgcct gggcctcgaa attcagcatg acgcgatggc ggagcaccgg
     3301 cagcatgcta tccagaatat cctccaccgc gacgtgaaaa cgcccgcgca gcagggcccg
     3361 gatttttgcc gccagaatca acgcctgtcc cgcccgcgga ctggcgccat aacgaacaaa
     3421 tcgcttgatt tcgccggatg cctcggcggt ttccggatga gtcgccatga cgagttccac
     3481 cgcccgccgg cggagcgttt cggccaccgg cacggcccgg gccagttcac ccatggcgat
     3541 caactcatgt ccgccgaaca aggccttcac atccgtatgc gtcttctccg tggttcgccg
     3601 gagaatttcc tcaaactcct ccatgcgcgg gtaaggcact ttcaatttga aaaaaaagcg
     3661 gtcgagctgc gcttccggca agggataggt tccctcctgc tccaacggat tctgcgtggc
     3721 caatacaaaa aacggcgggc cgagtggacg cgtttccccg gctaccgtga cgtgatgctc
     3781 ctgcatggcc tcaagcagag cggattgggt cttgggcgtc gcccggttga tttcgtcgcc
     3841 gagcagcaga ttgcaaaaca aggggccgcg cgagaattcg aaacgcgaat gcccgtggcc
     3901 atcgtccacc agaatgttgg tcccgatcaa atccgccggc atcagatccg gcgtgaactg
     3961 gacccgctga aaactgagct taagcgcgtt ggcgatcgat cgaaccagca tggttttgcc
     4021 caatcccgga accccctcca gcaaaacatg gccgcccccg ataatggcga tcaacacatt
     4081 ctcgataatc tcatcctgcc cgacgatgac cttttgaatt tcagttttga gttggacaaa
     4141 tttatttcgg aattcaacgg cgtcggcgga ggcgtcagga ttcataaagg catcagggag
     4201 ccggctgtcc gcgaatcatt tcaaaatacc ggcggatggt ttgacggtgg gcgagcggca
     4261 gggattcatc ctccaccgcc tcctcggaaa gttttcggta ggcatcgaga ttcgccgtgc
     4321 tcacggatac cgtttgctgc cgaggcgcct cctcgctgct gacaatcgaa gtttcggatt
     4381 cacccgattc gttgctggtt cccgaaactt gctcatgcac gcccgccggc atttgatcgg
     4441 tggggaggat ctgacggttg ggatcagttg ccgaaccggc gcctttgccg gggttctggg
     4501 acttcaccaa cgaaagcttg ggcagcagcg acatgccctg gcacatggat tcgccatttc
     4561 ccaggcagga tttgcacatg ccgagctgat tgcattgcag cgcaagtttt ttgcattgct
     4621 gccccaaggc gttttgctgc ccgagggatt ggcacatttt tttcaatccg tccttaaaat
     4681 ccgaggaact cgaatttttt cccgcctggc tcaaggactg cacggcctgc cccatggccg
     4741 attgaccggc ttgcgagagt ttttgaccca gcttcgaaag tttttcctgc gtttccgccg
     4801 gttgcagggc cggaggaaga ctggcggtgt ccgccgcctg cttggcttcg agttcccgtg
     4861 cggccttttc ggcgccctca taatcccgct ccttcagggc tttcgcgaga gcctccatgc
     4921 ccccggcctg ccccaaggct tcggataagt cggccgcaaa tgaatcgagg gattgagcag
     4981 caagtttttg ctgaacctga gcaatcttgt cctccagttt gctcagttcg agaaaggttt
     5041 cacggggagt cgcctttcct tcagccaact gcttccgaaa aggttccagc tctctgagca
     5101 tctgttccag gacggggtcc gaatctttct gctctgcctg ctcccagtcc ttaaaaagat
     5161 cctccaaatc ggcggcttgt tctccggaaa gcgccacctc gggaaccatg gcccgcgagg
     5221 cattggttac gggcaggaat aatccgatca ttgcgacaat cagacagctc attgccaacg
     5281 cggaaaaaac tttcccggag gacgggagcg gctgccgctt cgaattcttc agccgttgga
     5341 tcgtatcggc aagctgcaga tttttccact gttccgaaag ctccgcatct tgaaattcga
     5401 tcgcggaaag aatggaattg ggcaggcggt attcgcgatc catccattgc cccaaacggg
     5461 atcggcgaat cggcagaaac ccaatcagtc caagtccccc aattgtcaat gtggcgagga
     5521 tccccaccac ccaaacgctt aacggtgcaa gagccggcag aaaaaaacgc agcgcgcacc
     5581 aaccgagaag gagcgcaaaa gcgccctgca aaccccatcc gccgtgatta atgacccgat
     5641 gccagcgata acgccgctcg gactccgcga caaatcgctt gaaatcatcg aaatgatccg
     5701 gggtcattcc cggcaattag cggaaaattt ccgccaagtc cattcttggt ttaattagaa
     5761 accaagaaag cgaggcgcct cgcttaggaa tctttggcga cgagcttgtt gaaacggctc
     5821 ttgcggcggt cggcggcgtt cgcgtggatc accccgcgct tggcggcttt gtccagggcg
     5881 gcggaaacgc tcttcaggcc ggcctgagcg gcggctttgt ccccgctcgc gagcgcggcg
     5941 cggatttttt tctgctcggt tttgatgccc gtgatgatgc cggcattctg gtcgtgacgg
     6001 cgcttgctgg tcagcgcgta tttggcggcg gatttggtat tggccatggg aatttaaggt
     6061 taaatgagaa aggttgaaga atgaaattgg tgccagagag aggaatcgaa cctccgacca
     6121 agggcttatg agtcccctgc tctaccgctg agctaccctg gcgatttatg ggtgggaaac
     6181 cgtagcaagt tcgggagcgg atgcaaggaa actcagccag ccggcccgat tttgcgaatt
     6241 ttcaaaaaag cgtggtactt tcgtggaggc agattaattt attcggaaat gtcgccaacc
     6301 cccttttccc agacgctaat ctggcggcga cttgatcccc ttcactctca attatggtcg
     6361 aagttcagat tcatggtttt tcatttgaaa gatgggttcg tggcaccctt tttgaaggat
     6421 actctggaaa ctacatgcaa aaatgggaca tacctcccga ttttaatcag catgagagca
     6481 tccctgagca attccgcaat attcctgttt cggtgaaatc ggcaaagtat ggctcaccca
     6541 ttgggcttgg cgatatctta cgacagcgtg ttatttacca accgtttctg atgattgccg
     6601 gcttttggag gcagcggact ccttcagaaa agtggattga agatatcgga gcagcgtttt
     6661 tcgatcctgc ccaatggaat ggattatggg gcggcctcac ctcggaaggc atacaagtga
     6721 tcgacgctca gatcaaagac ctaggcacgc cctacacggt cgcaagagag ttggcacagc
     6781 agtggaaacg gcgattagca gatacctcgg gcagcgctgt tgtcgttaac ccaaagattg
     6841 actcgaagcg gcagcgcaga attcaatgct ctcttccatt tcggacgttc tgggaggcag
     6901 ctggacggaa acctactgtc tgcgagtcac ctgcactatt tggcttcgag ttcgagaatc
     6961 cgattctttc aggagccaga actttcaatc aagattgaac tctggcacag agaggatttt
     7021 cttgatccga ttttccgcga ttgccacgta ttccggattc acctctattc caacccaccg
     7081 tcttccaagt tcctccgcag atgccgcgct tgtcccactg ccaaggaacg gatcgagcac
     7141 ccaatcacct ttccgactac tcgaagagac aagctgattg atcagtgcga tcggcttttg
     7201 tgatggatgg ccgatttttt ctttcgatga acctttcagt gaaggaaccc gcaaaatgtt
     7261 cgtagcgtat ttgccattcc gaagatgcct ttcgcgagct tccttgtcct tgtagcgctt
     7321 atcccgaagg taggattgga ttttatcctc gtcatacggg aggcgcacag cgcccttatc
     7381 gaagtgtggt tttgagttgg ccgcatggcg caacacaagg atcatctcat actgcggcgt
     7441 aaaggagtca tacctttcat tgtaaccaac cgctctatcc cagatgatca tgtcatgaaa
     7501 ctgcatctct tttgcgagca tagagcaggt gtggagccag acgtgttcac gcttgcccag
     7561 ttgtccaaag atgtagagaa gtccatcctg acaaagaacc cttttgcact cacggaccca
     7621 attcagtgtc caatccaagt aagcatcaga agattgccaa gtgttatccc acttctcctc
     7681 aagaagaact tggaagtatg gtggatcggc aataatcgtt tggaactgat tggcttcaag
     7741 agtgtgaagc acattcaagg catccccaat atggatttct ccgtttccca cactgacgat
     7801 ttgtttcgat gatgggtgat acatacgctc atcttctata attattgcgc taacgaaacc
     7861 tttttccgct ggccgaccaa tatttttccg accgtgatgc gcaaaccact ctagatcagc
     7921 ttcttgaatt aagtgatctc taccccgttt ttcggcaggg atacgaccat ccttgatcaa
     7981 tttcctaaca cg
//
LOCUS       DATDAY010000413         3765 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_861774, whole genome shotgun sequence.
ACCESSION   DATDAY010000413 DATDAY010000000
VERSION     DATDAY010000413.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 3765)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3765)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3765
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_861774"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(57..1559)
                     /locus_tag="VIS74_08155"
     CDS             complement(57..1559)
                     /locus_tag="VIS74_08155"
                     /inference="COORDINATES: protein motif:HMM:TIGR03663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase activity-associated protein Agl23"
                     /protein_id="HEY5793256.1"
                     /translation="MQLTKTDWVILALALLLRVWGLDGKPPHFDEGINGWFVDQMKRL
                     GFYEYDPHHYHGPLHFYVLFVSDALFGRNVWALRLPVVIVSFFSVVWMLKYRDFLGRR
                     AAQVAAVAMAVSPAFVFYGRYSIHEAWQVFFNLILVWGALGLWLRGEKRHLYALVAGL
                     TGLILTKETYVIHAGSVLLAFGALKVWEMIVRSEPAQPWAAPQWTPADLLRSSAIGLG
                     VIVFFYSGTFLNLPGVAGLGETFSQWFTQTVAEPKHEKSWAYWLELMARYEWPALAGL
                     ACGVRYLPPSRAPARLLAILGAGVLAAYTLIPYKTPWCLMSILWPFYFVLGAALGEVP
                     PRWRKAAGLAAAALILYSAFLSIRLNFFRCTDSREPYVYVQTSPEITTLTGPLFEWAK
                     QNPAYYEATGAILLESYYPLPWILGDFSRIGYYGAHETDPQKPDNWPAILDQDFIVIE
                     KAKLDEALPRFRGDYLEREFELRDGMGACAAFFRRDKFPGLESIKKAEAP"
     gene            1596..2312
                     /locus_tag="VIS74_08160"
     CDS             1596..2312
                     /locus_tag="VIS74_08160"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY5793257.1"
                     /translation="MSSNQEQPEISLVIPARDEAGRLPATLDACQRAMANWPFASELI
                     VFAEPGGDDTAEIAESFAGVRVLRGDHPRGKGFAVRAGLRAARGAFAHFFMDADLSVP
                     LDFVPIFVEEFQRDPAVDVLFGSRRHPQSKVIISQPWQRVASGRLFNWVMRGAGLTRF
                     RDTQCGFKAFRAEWTEKIFSRATLDGFAFDVEILRIAQQLGAQVREMPVDWMEYGGST
                     VHMGNGVRAVLDALSLKSKK"
     gene            2778..>3765
                     /gene="dcm"
                     /locus_tag="VIS74_08165"
     CDS             2778..>3765
                     /gene="dcm"
                     /locus_tag="VIS74_08165"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: protein motif:HMM:NF012372.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HEY5793258.1"
                     /translation="MELCAGGGGQALGLEWAKFHHLACVEYEPQFCTTLRKNRPHWNV
                     MQDDIRNVEGSRFAGVDLIAGGVPCPPFSVAGKQLGAQDERNMFPAALQIIADAKPRA
                     VLLENVPGLASARFSDYRRELFKALNGMGYQVFSRLLQSSNFGVPQLRPRFLIVALRA
                     REAEFFNWPHEPSPARTVGDALADLMAENGWLGAEAWASRANDVAPTLVGGSRKHGGP
                     DLGPTRAKQQWAKLGVDGVGIADSAPLPAFPVDGKPRLTLRMAARVQSFPDEWIFTGG
                     KTAAYRQIGNAFPPRVAHAIGASIFAALKLEKLQSAKACLENTEFKLFEKAPV"
BASE COUNT          804 a         1026 c         1177 g          758 t
ORIGIN      
        1 ccaagcgcct gagcgtaacc aaaggcgctt ggcacaagcg ccgctacaat gctttttcat
       61 ggggcctccg cctttttaat ggattccagg ccggggaatt tatcgcggcg gaaaaaggcg
      121 gcgcaggcgc ccatgccatc gcgcagttcg aattcacgct caaggtaatc gccgcgaaaa
      181 cgcggaagcg cttcgtcgag cttcgctttt tcaatgacga taaaatcctg atccaaaatc
      241 gccggccaat tgtcgggctt ctggggatcc gtttcgtgtg cgccgtaata accgatccgc
      301 gaaaaatccc cgagaatcca cggcagcgga taataactct cgagcaggat cgcgccggtg
      361 gcttcgtaat acgccgggtt ctgcttcgcc cattcgaaca gcgggccggt cagggtcgtg
      421 atttccggcg aggtttgcac atacacgtag ggttcgcgcg agtccgtgca gcggaaaaag
      481 ttcagccgga tgctgagaaa cgcgctgtaa agaatcagcg ccgcggcggc caagcccgcc
      541 gccttccgcc agcgcggcgg aacctcgccg agcgccgcgc ccaggacgaa ataaaacggc
      601 cagaggatgc tcatcaggca ccacggcgtc ttgtagggaa tcagcgtgta ggcggcaagc
      661 acgccggcgc cgagaatggc cagcagccgc gccggagccc gtgaaggcgg caaataacgg
      721 actccgcaag ccaggccggc cagcgcgggc cattcgtagc gcgccatcaa ttccagccag
      781 taagcccacg atttttcatg cttcggctcg gcgaccgtct gcgtgaacca ttgcgaaaac
      841 gtttccccga ggcccgccac gcctggcaga tttaagaatg tgccggagta aaaaaagacg
      901 atcacgccca gcccgatggc cgagctgcgg agcaggtccg cgggggtcca ttgcggcgca
      961 gcccagggtt gggccggctc ggagcggacg atcatttccc aaaccttcag cgcgccaaag
     1021 gccagcagaa cgcttcccgc gtggatcacg taggtttcct tcgtgaggat gaggccggtc
     1081 aatccggcca cgagggcgta gaggtgccgt ttttccccgc gcagccacag cccgagcgcg
     1141 ccccagacga ggatgagatt aaagaaaacc tgccaggctt cgtggatgct gtaacggccg
     1201 tagaagacaa acgcgggcga gacggccatg gccacggccg cgacctgcgc ggcgcgccgg
     1261 ccgaggaagt cgcggtattt cagcatccag accacgctga aaaaactcac gatcaccacc
     1321 ggcagccgca gcgcccagac attgcgtccg aacagcgcgt cgctcacgaa gaggacgtaa
     1381 aaatggaggg ggccgtggta gtgatgcgga tcgtattcgt agaacccgag acgcttcatc
     1441 tggtccacga accagccatt gatgccttcg tcgaaatgcg gaggcttccc atccagcccc
     1501 caaacgcgta aaagcagggc tagcgcgaga ataacccaat ccgtttttgt cagttgcatc
     1561 cacgctctcg ccacgggaaa cttggtagat tcagaatgag ttcaaatcaa gagcagccgg
     1621 aaatttcgct ggtgattccc gcgcgcgatg aagccggccg cctgcccgcc acgctggatg
     1681 cctgccagcg cgccatggcg aactggccgt ttgcgagcga gctgatcgtt tttgcggaac
     1741 ccggcgggga tgacaccgcg gaaattgcgg aaagctttgc cggcgtgcgg gttttgcggg
     1801 gggatcaccc gcgcggcaag ggattcgccg tccgggccgg cctgcgcgcc gcgcgcggcg
     1861 cgttcgccca ttttttcatg gacgccgacc tgagcgtacc gctggacttc gtgccgatct
     1921 tcgtggagga attccagcgc gatcccgccg tggacgtgct cttcggcagc cggcggcatc
     1981 cgcaaagcaa ggtgatcatt tcccagccct ggcagcgggt ggccagcggg cggctgttta
     2041 actgggtcat gcgcggcgcc ggcctgaccc ggttccgcga cacgcaatgc ggtttcaagg
     2101 ccttccgggc ggaatggacg gaaaaaatct tctcccgcgc cacgctcgat ggctttgcgt
     2161 tcgatgtgga gattctccgg atcgcccagc agctcggcgc ccaggtgcgc gaaatgccgg
     2221 tggattggat ggaatacggc ggctcgaccg tccacatggg aaacggcgtg cgggccgtgc
     2281 tcgacgcgct cagtttgaaa tccaaaaaat aatctccgct atgggagatg ttttcagcaa
     2341 aaagaaacgt tcggaggtaa tggcgtcaat ccgttctacc ggcaatcgcg aaacggagtt
     2401 aacattggct cgcattttcc gggcagcagg catttgcgga tggcggcgaa gggttccttt
     2461 gtttggcaaa ccagattttg tgttccggcg cgaacgagtg gctgtttttg tggacgggtg
     2521 tttttggcat ggttgtccgc tgcatggacg gattccaaaa tcaaacaatg agtattgggt
     2581 gccgaaatta aggcgtaaca aactcaggga tcgcgaagtg actaagcacc ttagccgatt
     2641 gggttggaag gtgattcgtc tttgggatca tgacttgaaa aaacctgaac attccgcgag
     2701 aaaggtaatc cgtgcactcc gccatctcac ctcgaattga ttccaagatt ggacgccaag
     2761 gagaaagcgt gacatgtgtg gagttatgtg cgggtggggg ggggcaagct cttgggttgg
     2821 agtgggcaaa gtttcaccat ttagcttgtg tggaatatga gccgcagttc tgcaccacac
     2881 tgcgaaaaaa ccgtccgcat tggaatgtta tgcaggatga tattcgcaat gtggaaggca
     2941 gccgcttcgc gggcgtggat ttgattgcgg gcggggtgcc ttgtccgccg ttcagtgtag
     3001 cgggcaagca attgggggct caagatgaac gaaacatgtt tcccgcggcg ttgcagatta
     3061 ttgctgatgc caaaccgcgg gccgtgcttc tggaaaatgt tccagggctc gcatccgcac
     3121 gtttctcgga ttatcggcgg gaactattta aggcattaaa tggaatgggc tatcaggtct
     3181 tcagccgctt gctgcaatct tccaattttg gggtgcctca gttgcgtcca cgctttttga
     3241 ttgtggcgct gcgggctcga gaagcggagt tttttaactg gccgcatgaa ccaagcccgg
     3301 ctcgaacggt gggagatgcg ctggcggatt taatggctga aaatggctgg ctgggggctg
     3361 aggcttgggc cagccgtgcg aacgatgttg cacccacttt agttggagga tcacgtaaac
     3421 acggcggccc cgatttgggg ccaacccgag ccaaacagca gtgggctaaa ttgggggtgg
     3481 acggtgttgg gattgccgac agtgcgcctc tgccggcttt tcctgtggat gggaaaccgc
     3541 gtcttacttt gagaatggcg gcaagagtgc agagctttcc agatgaatgg atttttacgg
     3601 gtgggaaaac cgcggcttac cggcagatag gcaacgcctt tcccccgcgg gttgctcacg
     3661 cgattggcgc gtccattttt gctgcattga agctcgaaaa gttgcaaagt gccaaagcat
     3721 gcttggagaa tactgaattc aaattgtttg aaaaagctcc cgtgt
//
LOCUS       DATDAY010000414         4977 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_1738292, whole genome shotgun sequence.
ACCESSION   DATDAY010000414 DATDAY010000000
VERSION     DATDAY010000414.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 4977)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4977)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4977
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1738292"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..190)
                     /locus_tag="VIS74_08170"
     CDS             complement(<1..190)
                     /locus_tag="VIS74_08170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HEY5793259.1"
                     /translation="MNVADLREILQYVPKFRGKTFIVAIDGQVVESENFSNILLDLAV
                     LRSLNIKVAIVHGASHQIT"
     gene            354..857
                     /locus_tag="VIS74_08175"
     CDS             354..857
                     /locus_tag="VIS74_08175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEY5793260.1"
                     /translation="MPLPELEAIYDAHAQALFAFALSLTRSEALAQDALQEIFCKLAR
                     RPQLLARARNPRAFLLRMTYHWVVDEARRDQARIRRTEALPAPPQFAASPDPDESLFR
                     QELESALGELPPEQRAVVHLKLWGGLTFESIAETLRIPANTAASRFRYGLDKLQTRLR
                     PLYEEIQ"
     gene            854..1216
                     /locus_tag="VIS74_08180"
     CDS             854..1216
                     /locus_tag="VIS74_08180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5793261.1"
                     /translation="MNPDSIETRLAQIPLRELPPEWKQAVLARSRRTATPAPLRLNFR
                     DWLWPHPWAWAGVAAAWLLIAGLNCSGPRGGALYAIHPAQRISTEQYAAYVRVRDRFL
                     AQNHPPEPRAAWPPPAEL"
     gene            1376..2929
                     /locus_tag="VIS74_08185"
     CDS             1376..2929
                     /locus_tag="VIS74_08185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5793262.1"
                     /translation="MKTFLRWALFALVLLATLIVLTYSLIGFQGRREWAAAQAELRAK
                     GEPLSLAELVPPPIPDEVNFFAAPIWREATDFETKTYYPMGKPDTGRVPKTDPKTWRL
                     QAFTTALENSQRESVLSKNGGTIEKGTRFNLDAAAEFYRAKLGLPPGLSSGETVLQGL
                     DTARAELTEIEAAARRPGARFPIPYEFTVAASVPHISPLLKIAQYLRIRIAAEAAEGK
                     GGDAEDHLFLMFYLADSLQREPLLISQLVRLSILNLAAEAVGEGIARNGWDAGQLQAI
                     QRRLAQVDISHDLAFALRGERGTFNQTIEPLAQGGMKKLAGLLQVATQLTSETPPPPM
                     LENFLTHFFDKAYPRGWVFSDLAYYDRIMQRGIEEGSAFEQPAFWKDVIEPEIIAKKQ
                     LPQGLTRLFSLIALPAIGGSTRQFLKTEIRLRQAQIACALERYRLAKGSYPDSLDALV
                     SEYLPALPLDACDQKNFRYQRPAPDQFVLWSIGPDKIDQGGTPPARASDPANDWVWRT
                     AQPERINRR"
     gene            3099..3461
                     /locus_tag="VIS74_08190"
     CDS             3099..3461
                     /locus_tag="VIS74_08190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5793263.1"
                     /translation="MQIRAYPNSSYSCDFALQSRVNEIVARKNRFTRYATGLSILLLA
                     TALLTGFLRLDAVSLGCVALLAISWAVAYFFLASPGIRSQCPHCHKRFKSVWVRDEEF
                     LVCENCHQYVFTHRRTVA"
     gene            3752..4090
                     /locus_tag="VIS74_08195"
     CDS             3752..4090
                     /locus_tag="VIS74_08195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5793264.1"
                     /translation="MSLTRLQRANLRFYLKFKDRPISLPGIIGFNRMLYLWLIGIFAL
                     VAFLLTLIAGSWAGLFVLSFLAGGLIRDLGFYRRTIHFWPIAKRIIHWETVEELVRDG
                     EPKPKTEPGA"
     gene            4197..4628
                     /locus_tag="VIS74_08200"
     CDS             4197..4628
                     /locus_tag="VIS74_08200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5793265.1"
                     /translation="MNPPRLTPPRPEEVVRLENIPQGSPHFFPYSTEQRMVSLTEGEL
                     RHFLKKGALSANDAHTTDALRDLAPFSLPNHINRTEFPDLEAWAARNKPNLALFLVCA
                     GAFSTRDGTVYFWRKLNDRVLYLATQKASCYLVLPKTKDGQ"
     gene            complement(4767..>4977)
                     /locus_tag="VIS74_08205"
     CDS             complement(4767..>4977)
                     /locus_tag="VIS74_08205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5793266.1"
                     /translation="HFSKMDSVPAGGHGPQFWKFLLRTALKTLSRPRPGMKKSPADDE
                     MSMLDDEKNILSRMKRASSMRKSAS"
BASE COUNT          955 a         1575 c         1374 g         1073 t
ORIGIN      
        1 gggtgatctg gtggctcgcg ccgtgcacga tggcgacctt gatattcagc gagcgcagga
       61 ccgcgagatc gaggaggata ttggaaaaat tctccgattc gaccacctgt ccatcaatgg
      121 ccacgataaa agttttgccc cgaaatttcg ggacgtactg caaaatttca cggagatcgg
      181 cgacgttcac ggattggaac caagagcatt taccacagcg ggcacgggga gcacagcggt
      241 tttcggaata ttttggagga aatttgtccc cgggaggccg gcggattgcc ggcctcccgg
      301 tttagacaaa aatcatcgcc cgcttcgtcc ggtttttata aggaatttcc aacgtgcctc
      361 tcccggaact cgaagcgatc tacgatgcgc atgcgcaggc cctctttgcg ttcgcccttt
      421 ccctgacccg ttccgaggcg ctcgcccagg acgcgctgca ggagattttc tgcaagctcg
      481 cccggcggcc ccaactcctg gcccgcgcgc gcaatccccg cgcgtttctg ctgcgcatga
      541 cctatcactg ggtcgtggac gaagcgcgcc gggaccaggc ccggatccgc cggaccgagg
      601 cgctgcccgc gccaccccag tttgcggcct cgcccgatcc ggatgaatcc ctgttccggc
      661 aggagctgga aagcgccctc ggggagctgc cgcccgagca gcgcgcggtg gtccatctca
      721 agctctgggg cggcctcacg tttgaatcca tcgccgaaac gctgcgcatc cccgccaata
      781 ccgcggccag ccgattccgc tacggcctag acaaattaca aacgcggctc cgtccgcttt
      841 atgaggaaat tcaatgaacc cggattcgat tgaaacacga ctggcccaaa tcccgctgcg
      901 ggagctgccg cccgaatgga agcaagccgt gctggctcgt tcccggcgga cggccacgcc
      961 ggcgcccctc cgcctgaatt tccgggactg gctctggccg catccctggg cctgggcagg
     1021 cgtggccgcc gcctggctgc tcatcgccgg gttgaattgc tccggcccgc gcggcggggc
     1081 gctttacgcc atccatcccg cccagcgcat ttccacggag caatacgccg cttacgtgcg
     1141 ggtccgggat cgattcctgg cccaaaacca cccgccggaa ccgcgcgccg cctggccgcc
     1201 gccggcggaa ctttagagcg tttttgatta cctttgtgaa catacagcgc catcgagaaa
     1261 aacacggcgg ggtcatcgac cccgccctgc ccttaaaagg cagggacggc tcgccaagcc
     1321 gtccgtcgct ttcggccgca tcgagccaaa tcgaaccatt ccccatccct cccccatgaa
     1381 aaccttcctt cgctgggcgc ttttcgccct ggtcctcctc gccacgctga tcgttctgac
     1441 ctacagcctg atcgggttcc aaggccgccg ggagtgggcc gccgcccaag ccgaacttcg
     1501 cgccaagggt gaaccgctga gccttgcgga attggtgcct ccgcccatcc cggatgaggt
     1561 caatttcttc gccgcgccga tctggcggga ggcgaccgat tttgaaacga agacctatta
     1621 tccaatgggt aaacccgaca ccggccgggt gcccaaaacc gatccaaaaa cctggcggct
     1681 ccaggctttc accaccgcgt tggaaaacag ccagcgtgag tcggttcttt caaaaaacgg
     1741 cgggactatc gaaaagggaa cgcgcttcaa tttggatgcc gccgcggaat tctaccgcgc
     1801 gaagcttggc ctgccgccgg ggctttcctc cggggaaacc gtgctacagg gcctggatac
     1861 cgcccgcgcg gaactaacgg aaatcgaagc cgccgcccgg cgccccggcg cccgttttcc
     1921 cattccctac gagttcaccg tcgccgccag cgtgccgcac atttcgcccc tgctcaaaat
     1981 cgcccaatat ctgcgaatcc ggattgccgc cgaagccgcc gaaggaaagg gcggggacgc
     2041 cgaggatcat ttatttttaa tgttttacct cgcggattcg ctgcagcgcg aaccgctttt
     2101 aatcagccag ctcgtccgcc tgtccatcct caacctggcg gcggaggcgg tgggcgaagg
     2161 catcgcccgg aacggatggg acgccgggca actccaagcc atccagcggc ggctggcgca
     2221 ggtggatatt tcccacgacc tggcattcgc gctgcggggg gaacggggaa cgtttaatca
     2281 gaccatcgag cccctggccc agggcggcat gaaaaaattg gcgggcctgc tccaagtggc
     2341 gacccaactc acttcggaaa cccctccgcc tccgatgttg gagaattttt tgactcactt
     2401 ctttgacaag gcttatccgc gcggctgggt cttctccgat ctggcttatt acgatcggat
     2461 catgcagcgc ggcatcgagg agggaagcgc gttcgaacag ccggcattct ggaaggacgt
     2521 catcgagccg gagataatcg cgaaaaaaca actgcctcag ggtttgaccc ggctttttag
     2581 tttgattgcg ctgccggcca tcggcggaag cacccgccaa ttcctgaaaa ccgaaatccg
     2641 cctccgccag gcccaaatcg cctgcgcgct cgagcgctat cggctcgcca aggggagtta
     2701 cccggattcc ctggacgccc tcgtttccga gtatctgccg gcattgccac tggacgcctg
     2761 cgatcaaaaa aacttccgct accaacgccc cgccccggat caattcgtcc tctggtccat
     2821 cggccccgac aagatcgacc agggcggcac tccgcccgcc cgggcgtccg atccagccaa
     2881 tgactgggtc tggcggacgg ctcagccaga aagaatcaac cgcagatgac gtcacagatt
     2941 ttcgcagata taccgcggct tattttacaa aatctgcggt tataaatccg ggttccctga
     3001 atgggggggg cggggcgcgc cggcggccgg gccatcaatt tttatcgaat tggctttgga
     3061 ttgaggcgcg ttttgttatc ccatgaagca agtggattat gcagattcga gcttatccca
     3121 atagcagcta ctcctgcgat ttcgcgctgc agtcgcgcgt caacgaaatc gtcgcccgaa
     3181 aaaaccgctt cacccggtat gccacggggc tttcgatcct gctcctcgca acggctctgc
     3241 tcaccggctt tctaaggctg gacgcggtct cgctcgggtg cgtcgcctta cttgcgatct
     3301 cctgggcggt cgcttatttt ttccttgctt cgcccggcat tcgaagccaa tgcccccatt
     3361 gccataaacg gttcaaatcc gtctgggtcc gcgatgagga attcctggtc tgcgaaaact
     3421 gccatcaata tgttttcacc catcgccgaa cggtggcttg aagtcgcgcg gaacgtttac
     3481 ccatggaggg cggcgcctcg tcgccgccgt gatttttccc aaggacctga gagagaatcc
     3541 gaaaaaacgt caggctcgtc tcgttgtttc acggttttca tttctggggg cttccatcca
     3601 gtcacggcgc cgacggagcg gcgccctcca cggataaaaa tgcccggcgg tgcaaaaacc
     3661 gcttggccca accgtcccgg caactgtctt cctttttaga aacctttttt gcgttctctg
     3721 cgttctttgc ggttaacctc tgcctcccga tatgtcactc acccgcctcc aacgcgccaa
     3781 tctgcgcttt tacctgaagt ttaaagaccg gcccatatct ctcccgggaa tcattgggtt
     3841 caatcgaatg ctgtaccttt ggctgatcgg cattttcgcg ctcgtcgcgt ttttactgac
     3901 attgatcgcc ggatcttggg cggggctgtt tgtcctaagc tttctggccg gcggcttgat
     3961 ccgggatttg ggattttacc ggcgaaccat tcacttctgg ccgattgcga aacggatcat
     4021 tcattgggaa acggtggagg aattggttcg cgacggcgag cccaagccga aaaccgaacc
     4081 gggcgcctga gcggaatggc ggccgcttac ccacggcttt acccggcgag cccatgagat
     4141 gccttgttcc ctttcttctg ctgatcgccc tcctcccggc gccggggttc gattggatga
     4201 atccacctcg cttgactccc ccgcgccccg aagaggtcgt gcgtctggaa aatatcccgc
     4261 aaggttcccc gcattttttc ccctattcga cggagcaacg catggtcagc cttaccgaag
     4321 gtgagctccg gcatttcctg aagaagggag cgctttccgc caatgatgcg cataccactg
     4381 acgctttgcg ggacttggct cctttttctc tcccgaacca tattaaccgg actgaattcc
     4441 ccgatctgga ggcttgggcc gctcgcaata aaccaaatct cgcccttttt ctggtttgcg
     4501 ccggggcatt ttccacgagg gacgggaccg tctatttttg gcggaaattg aacgaccggg
     4561 ttctttatct cgcgacccaa aaggcatcct gctatctggt tcttccaaaa acaaaagatg
     4621 gccaatgagc cgaacgggac ggtccccttc tctcgcatga ctcgacgaac ccgaagcttg
     4681 agccggaatg gccttttccc ccagtgaggg agtgtttttc ccgggcaagg aagctttttg
     4741 cctccttgag gatgctcttt tcaccctcaa gaggcgcttt tcctcattga ggatgctctt
     4801 ttcattctcg ataggatgtt tttctcatca tcgagcatgc tcatttcatc atcggcgggg
     4861 ctttttttca tcccggggcg ggggcgggat agagttttta gagcggttct cagaagaaat
     4921 ttccaaaact gcggtccatg accgccggcg ggaacggaat ccattttcga gaaatgc
//
LOCUS       DATDAY010000415         2194 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_74081, whole genome shotgun sequence.
ACCESSION   DATDAY010000415 DATDAY010000000
VERSION     DATDAY010000415.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2194)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2194)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2194
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74081"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..340)
                     /locus_tag="VIS74_08210"
     CDS             complement(<1..340)
                     /locus_tag="VIS74_08210"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HEY5793267.1"
                     /translation="MNPLLLLVDFQGDYLNAPGLEPAAGAVVERAAALLAGARAAGLP
                     VAHVWTTVRPEDDRRMPHWKTLGKWLCVEGTPGHAPPEPLRPLDSEPVIHKRFFSAFS
                     SGTLGPLLAER"
     gene            complement(347..1525)
                     /locus_tag="VIS74_08215"
     CDS             complement(347..1525)
                     /locus_tag="VIS74_08215"
                     /inference="COORDINATES: protein motif:HMM:NF012407.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HEY5793268.1"
                     /translation="MSHLRDIPPPARWIDSVLADGLRIPPSPPLVGVLTGEGIGPEVI
                     ANAIEVLHAVRGVTGCAVSLEYGAPIGRDAERGCGKPLSDEVITFCRDIFDRGGAILN
                     GPGGGRYVYDLRRQFDLFCKLSPLKVFPELADAIRLKPEQVRDIDILMVRENAAGLYQ
                     GRWRDHHLDAGRVAEHSFSYSEAQVRRILGPAARLAKTRRGHLTVVVKEAGVPGISGL
                     WRECALDVCARLGLACALLDIDHIAYRLIQHPREFDVVVAPNLFGDILSDLGGVLLGS
                     RGLCFAGSFSETGAAVYQTNHGAAYDLAGKDLANPVGQIFSLAMMLRESFGLAEEAAL
                     IEQAVARVWREGWRTADVAGMESRIIGTREMGRRIAEAMTQIPRILPALRPEMPAGRI
                     "
     gene            complement(1668..>2194)
                     /locus_tag="VIS74_08220"
     CDS             complement(1668..>2194)
                     /locus_tag="VIS74_08220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY5793269.1"
                     /translation="SDDLLAPGALQFVGSFFARNPTVDVVYGHRILINEGGAEIGRWI
                     LPPHCRETLRRIDYIPQETLFWRADLYHRVGGLDPSFTFAMDWDLILRFLSAGAVFRR
                     LPYFLACFRVHDLQKTSALTESIGITEVNRLRRRELGEDQDAWGVAEYDNRVKNRAFV
                     TELALKWGVRSTFI"
BASE COUNT          416 a          703 c          728 g          347 t
ORIGIN      
        1 ggcgctccgc gaggaggggg cccagggttc cgctcgagaa ggcgctgaaa aatcgcttat
       61 ggatcaccgg ctcggaatcc aacggacgaa gcggctccgg cggagcatgg ccgggggttc
      121 cctccacgca aagccacttg ccgagggttt tccaatgggg catccgccgg tcatcttccg
      181 ggcggacggt cgtccagacg tgggccacgg gaagaccggc ggcgcgcgcg ccggccagga
      241 gcgcggcggc gcgttcgacg accgcgcccg ccgcgggttc gagccccggg gcgttcaggt
      301 agtcgccctg gaaatcgacc agcaggagaa gcgggttcat gggaactcag atccggcccg
      361 ccggcatttc cggacggagc gccgggagga tccttggaat ctgggtcatc gcctcggcga
      421 tgcgcctccc catttcgcga gtcccgatga tgcggctctc catcccggcg acatccgccg
      481 tgcgccaccc ttcgcgccac acgcgggcca cggcctgctc gatgagcgcg gcttcctcgg
      541 cgaggccgaa gctctcccgc agcatcatcg ccagcgaaaa aatctggcca acggggttgg
      601 cgaggtcttt cccggcgagg tcgtaagcgg cgccgtggtt ggtttgatac acggcggcgc
      661 cggtctccga gaagctgccc gcgaagcaaa ggccccgcga tcccagcagc actccgccga
      721 gatccgagag gatgtcgcca aacaggttgg gcgccacgac gacgtcgaac tcgcggggat
      781 gctggatgag ccggtaggcg atatggtcga tgtccagcag cgcgcaggca agtcccaggc
      841 gcgcgcagac gtcgagggcg cattcgcgcc agaggccgct gattccgggc acgccggctt
      901 ccttgacgac aaccgtcaag tggccgcggc gggttttggc cagcctcgcc gccggcccga
      961 gaattcgccg gacctgcgcc tcgctgtagg aaaaggagtg ttcggcgacg cgcccggcgt
     1021 ccaaatggtg gtcccgccag cgcccctggt aaaggccggc cgcattttcg cgcaccatga
     1081 ggatgtcgat atcgcgaacc tgttccggct tgagccggat cgcgtcggcg agttccggga
     1141 agactttaag cgggctgagc ttgcagaaga ggtcaaactg ccggcgcagg tcatacacat
     1201 aacgtccgcc gcccggtcca ttgaggatgg cgccgcctcg gtcgaagatg tcgcggcaaa
     1261 aggtgatgac ctcatcggaa aggggtttcc cgcacccgcg ctcggcgtcc cgtccgatgg
     1321 gagcgccgta ttccagggaa accgcgcagc ccgtcacgcc ccgcacggcg tgcagcacct
     1381 cgattgcgtt ggcgatgacc tcgggcccga tgccttcgcc ggtcagcacg cccacaagcg
     1441 gcggggaggg agggattcgg agcccgtccg cgagcacgga gtcgatccag cgggcgggcg
     1501 gcggaatatc tcggagatgg ctcacaaatc catcccccgt ttgaatggcc gataattcgc
     1561 gaaaaccagc agcctaatga gggtttcggc agtagcgcgc attgacgcag cagataaacg
     1621 gctgcgcccg gcttgacaag cggggagtgg agggccgggc ctttatctca gatgaaggtc
     1681 gaacgcactc cccacttcaa ggccagctcg gtgacaaacg cccggttttt cactcggtta
     1741 tcatattcgg ccacccccca ggcgtcctgg tcttcgccca attcccgccg ccggagccga
     1801 ttgacttccg ttatcccgat gctctcggtg agggcggagg ttttctggag atcgtgaacg
     1861 cgaaaacacg ccaggaaata gggaagccgg cggaaaacgg ctcccgccga cagaaatcga
     1921 agaatcaagt cccagtccat ggcgaaggtg aacgacgggt ccagcccgcc cactcgatgg
     1981 tagagatccg cgcgccaaaa aagggtttcc tgcgggatgt agtcgattcg ccgcagcgtt
     2041 tcccggcaat ggggagggag aatccatcga ccgatctccg cgccgccctc attgatcagg
     2101 atgcggtggc cataaaccac gtccacggtg ggattcctgg cgaaaaaaga cccgacgaac
     2161 tgcaacgctc ccggcgccag caggtcatcc gagt
//
LOCUS       DATDAY010000416         3564 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_1738445, whole genome shotgun sequence.
ACCESSION   DATDAY010000416 DATDAY010000000
VERSION     DATDAY010000416.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 3564)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3564)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3564
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1738445"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..542
                     /locus_tag="VIS74_08225"
     CDS             <1..542
                     /locus_tag="VIS74_08225"
                     /inference="COORDINATES: protein motif:HMM:NF023227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5793270.1"
                     /translation="WHELRGEAVYRPPAKRRTLGHSHVLAPDIRNETAARGVLHRLLQ
                     KAAMRLRHANYFAEGLQLSIRYQNGPSWSDELRFTGTQDTLELTRHFQQLWDRRRRAD
                     LPPLHVGVTFFSLVPAHLHTPSLFDDPQRESLFEAVDDLNLKLGKNTVFFGGAINALQ
                     HAPMRIAFGRIPDPRIEGD"
     gene            614..856
                     /locus_tag="VIS74_08230"
     CDS             614..856
                     /locus_tag="VIS74_08230"
                     /inference="COORDINATES: protein motif:HMM:NF013561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HEY5793271.1"
                     /translation="MIYDDVIMRTIVDLPDEQVKALAAFCRAEGISRAEAVRRALTRL
                     VDDQKGAQKEDSFGAWKERRFDSRRYVEELRREWGE"
     gene            853..1230
                     /locus_tag="VIS74_08235"
     CDS             853..1230
                     /locus_tag="VIS74_08235"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEY5793272.1"
                     /translation="MKAVIDSDVLIDYLQGVEAARRELNQYSELCYSVISWMEILIGA
                     ETPGEEKSARTFLASLRMIELTREIASQAVSERKRSKLKLPDAIILATADVEGCILVT
                     RNTKDFQKRDPRVRFPYQTETDL"
     gene            complement(1227..1634)
                     /locus_tag="VIS74_08240"
     CDS             complement(1227..1634)
                     /locus_tag="VIS74_08240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5793273.1"
                     /translation="MNYLLDVNILIAWGWSDHLDHGRTIHWIARQKKNSQVRLLTSTI
                     PEMGFVRVSAQRSAGQISVAEAGAALGGMLKSLGKSHDFLPDDLGGVFWPDWCRSPGR
                     TTDAHLLQLAVNHDAILATLDAAIPNAELLPFI"
     gene            complement(1631..1873)
                     /locus_tag="VIS74_08245"
     CDS             complement(1631..1873)
                     /locus_tag="VIS74_08245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5793274.1"
                     /translation="MATLSFKVSDEESRTIRAGARKAKLSVSEFLRRRAGAAPAAPAK
                     LERKRCPLTGAIIFAPASELPPLTVEATREMLADFP"
     gene            complement(1999..>3564)
                     /locus_tag="VIS74_08250"
     CDS             complement(1999..>3564)
                     /locus_tag="VIS74_08250"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HEY5793275.1"
                     /translation="VNQGGKRRGSGCAYLETWHNDIEDFLQLRVNVGDERRRTHDMNT
                     ANWIPDLFMKRMEARQGWTLFRANEVPDLHDAYGRKFEERYAHYEQLAKAGKIFGKEI
                     PAIDLWKQMLKMLFETGHPWITFKDPCNLRSPQDHAGVIHSSNLCTEITLNTSEDETA
                     VCNLGSVVLDSHLRENGTLDLDLLRETIEVAIRALDNVIDINFYPTESAKRSNLRHRP
                     IGLGVMGLQDALFKKGIGFASEEAVEFNDEFMEAIAFFAYGASSDLAAERGVYSSYRG
                     SKWDRGLLPQDTVELLEKERGVKIDVPHGGKLNWAEVRMKIRKQGMRNSNVIAIAPTA
                     TISNIMGTTPCIEPNYKNLYVKENLGGKFTILSTELVRDLKKLNLWNDDMIDQLKYFE
                     GELANIEGIPEDIKRKHLTVFQIDFRYVLDAAARRQKWIDQSQSVNLFLGTPDLKVLS
                     HMYRAAWHKGLKTTYYLRTLGASSIESSTATVKKELNGVVAAAKKKEYTETEKTACSL
                     EARLRGEECEACQ"
BASE COUNT          659 a         1017 c         1125 g          763 t
ORIGIN      
        1 agtggcacga gctgcgcggg gaagcggtat atcgcccgcc ggcgaagcgc cgcaccctcg
       61 ggcattcgca cgtgctcgcg ccggacatcc gcaacgaaac cgcggcccgg ggagtgctcc
      121 accgcctgct gcaaaaggcg gcgatgaggc tgcggcacgc gaactatttc gccgaagggc
      181 tgcagctttc gatccgctat caaaacggcc cgagttggag cgacgaattg cggtttaccg
      241 gcacgcagga caccctcgaa ctgacgcggc attttcagca gctgtgggat cggcgccggc
      301 gcgcggacct gccgcccctg cacgtcggcg tgaccttttt cagcctggtg cccgcgcatc
      361 tgcacacgcc ctcgctgttc gacgatccgc agcgcgaatc cctgttcgag gcggtggacg
      421 atctcaattt gaaactcggc aagaacaccg tctttttcgg cggcgccatc aacgccctgc
      481 agcacgcccc catgcggatc gccttcggcc ggattcccga tccgcggatt gaaggggatt
      541 gagtgaaatc tgcggatgga aatccggact ccgttttgat ttccatccgg aatgatttgc
      601 ttcacgtgaa gcgatgatat atgatgacgt catcatgcga accatcgtgg atctgcccga
      661 cgaacaggta aaagcgctgg cggcgttttg ccgggcggag ggaatctccc gggcggaggc
      721 ggtccggcgg gcattaaccc gtctggttga tgaccaaaag ggagcgcaga aggaggattc
      781 cttcggagcc tggaaggaac gacgctttga cagccggcgg tatgtggaag aactgcgccg
      841 ggagtggggc gaatgaaggc cgtcattgat agtgatgtct tgatcgacta ccttcagggg
      901 gtcgaagcgg cccggcggga actcaaccaa tattccgagt tatgctattc ggtgatttca
      961 tggatggaaa tcctgattgg cgccgaaacc cccggggagg aaaaatccgc ccgcacgttt
     1021 ttagcttcgc tgcggatgat tgaactgacg cgggaaattg cctctcaagc ggtcagcgaa
     1081 cggaaacgat ccaagctcaa gttgcccgac gcaatcatcc tggctacggc ggatgtggaa
     1141 ggctgcattc tggtaacgcg gaatacaaag gattttcaaa agcgagaccc gcgcgtgcgt
     1201 tttccctatc aaacggaaac ggatctttaa ataaaaggaa gcaattccgc gttgggaatc
     1261 gcggcgtcca aggttgccag gatggcatcg tgattcaccg cgagttgcag caaatgcgcg
     1321 tccgttgttc gtcccggtga gcggcaccaa tcgggccaga aaacgccccc cagatcatcg
     1381 ggcagaaaat cgtgggattt ccccagggat ttgagcattc cgcccagggc agctccggcc
     1441 tcggccacgc tgatttggcc cgcgcttctc tgcgccgata ctcgcacgaa tcccatttcc
     1501 ggtatggtcg aagtcagcag gcgaacctgg gagtttttct tctggcgggc aatccaatga
     1561 atggttcttc catggtccag atgatcgctc cagccccaag cgatcaggat atttacatcc
     1621 agcaggtaat tcatggaaaa tccgccagca tctcgcgggt ggcttcgacc gtgaggggtg
     1681 gaagttccga ggcgggcgca aaaatgatcg ctcccgttaa cggacaacgc ttccgttcaa
     1741 gcttggcggg cgccgcgggt gcggcgcccg cccgccggcg gaggaattcg gacacgctta
     1801 atttcgcctt ccgggctccg gcccggatcg tccgggactc ctcgtcggaa actttgaagg
     1861 aaagcgttgc catggtaata ttgtaatatg gggcggcggt gcgtcgaagc ggaatttttg
     1921 aaaaaactcc gcgggcaaac caaaaagccg ggctccgcct tcgcgggaac ccggcttttt
     1981 ttggtttgat tggatcctct actggcaggc ttcgcattcc tcgccgcgca ggcgggcttc
     2041 gagggagcag gcggtttttt cggtttcggt gtattccttt ttcttcgcgg ctgcgacgac
     2101 gccattcagt tcctttttca ccgtcgcggt ggagctttcg atgctgctcg cgccgagcgt
     2161 gcggaggtag taggtcgtct tgagcccctt gtgccaggcg gcgcgataca tgtggctgag
     2221 caccttgaga tcgggcgtgc cgaggaacag gttgacggac tgggactggt caatccactt
     2281 ctgccggcgc gcggcggcgt cgagcacgta gcggaaatcg atctggaaaa ccgtgaggtg
     2341 cttgcgcttg atgtcctcgg gaatgccctc gatgttcgcc agctcgccct cgaagtattt
     2401 gagctggtca atcatgtcat cgttccacag gttcagcttc ttgagatcgc ggacgagttc
     2461 cgtgctcagg atggtgaatt tgccgccgag gttttccttc acgtaaaggt tcttgtagtt
     2521 cggctcgatg cacggggtcg ttcccatgat gttggaaatg gtcgccgtgg gcgcgatggc
     2581 gatgacgttc gagttccgca tgccctgctt gcggatcttc atgcgcactt cggcccagtt
     2641 gagcttgccg ccgtgcggga cgtcgatctt cacgccgcgc tccttttcga gcagctccac
     2701 cgtatcctgc ggcagcaggc cgcggtccca cttggaaccg cgatacgagc tgtaaacgcc
     2761 gcgctcggcg gcgaggtcgc tcgacgcgcc gtaggcgaag aaggcgatcg cctccatgaa
     2821 ctcgtcgttg aattccaccg cttcctcgct ggcgaagccg atgcctttct tgaacagcgc
     2881 gtcctgcagg cccatgacgc cgaggccgat ggggcgatgg cgcaggttgc tgcgcttggc
     2941 ggattcggtc gggtagaagt tgatgtcaat gacgttgtcg agcgcgcgga tggcgacctc
     3001 gatcgtttcc cgcagcaggt cgaggtcgag cgtgccattc tcgcggaggt ggctgtcgag
     3061 cacgacggag ccgaggttgc agaccgcggt ttcgtcctcg ctggtgttca gcgtgatctc
     3121 ggtgcagagg ttcgaggagt ggatgacgcc ggcgtgatcc tgcggcgagc gcaggttgca
     3181 cggatccttg aacgtgatcc acggatgccc ggtttcaaag agcatcttga gcatctgctt
     3241 ccacaggtcg atggccggaa tttcctttcc gaagattttg cctgccttcg cgagctgttc
     3301 gtagtgcgcg taacgctcct cgaacttccg gccgtatgcg tcgtgcagat ccggcacctc
     3361 attggcgcgg aagagcgtcc agccctggcg cgcttccatt cgcttcatga acaggtcggg
     3421 aatccaattc gccgtgttca tgtcgtgcgt gcggcggcgc tcgtcgccga cgttcacgcg
     3481 gagctgcagg aagtcctcga tgtcgttgtg ccaggtctcg agatacgcgc agccggagcc
     3541 gcggcgcttg ccgccctgat tgac
//
LOCUS       DATDAY010000417         4113 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_686258, whole genome shotgun sequence.
ACCESSION   DATDAY010000417 DATDAY010000000
VERSION     DATDAY010000417.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 4113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4113
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_686258"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1772)
                     /locus_tag="VIS74_08255"
     CDS             complement(<1..1772)
                     /locus_tag="VIS74_08255"
                     /inference="COORDINATES: protein motif:HMM:NF028510.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cache 3/Cache 2 fusion domain-containing
                     protein"
                     /protein_id="HEY5793276.1"
                     /translation="MHRSLRTKVFIWLLIVLAGMTASGWIGYRQLSNYIHQQARVEMD
                     SKLDHVIDVLEATDTVYSNLVRASLEVLKMEARKLGEPRLVEGRLYFGDKAIDSSFEE
                     VDRVKRLMGGTATLFVKQGDRFIRVSTNVRRDDGTRAVGTELDPNGRAIAAIRRNESF
                     FGVVDILGKPYLTGYEPMYSAGGEVIGIYYVGYGLETLASIGRAIDNRALLQSGFFAL
                     VNPRDEVVFRTESRDIPADAAEVAERAARHLPVNGDWSILRHQFVPWDYEVVAALYLP
                     DIHSLTVRILWQVYGITGAILLVIVAASFWLASRLSAALALTETARQEALLARDAAES
                     ANRTKSAFLANMSHELRTPMNAIIGYSEMLIEDAEDGGNDAAIADLQKIRSAGKHLLA
                     LINDVLDLSKIEAGKMTLYLEEFSVDQMLGEVVSTVQPLLDKNQNKLDLQKDAGLGTM
                     KSDLTKVRQTLFNLLSNASKFTEKGLITLSAKTFSQQGSPWIQIRVADTGIGMTPGQL
                     GRLFQAFTQADDSTTRKYGGTGLGLVISRKFCQMMGGDISVESLAGQGSAFTVDLPLR
                     VGEPAAASPALALPPETPKRLVLI"
     gene            1857..2201
                     /locus_tag="VIS74_08260"
     CDS             1857..2201
                     /locus_tag="VIS74_08260"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEY5793277.1"
                     /translation="MAEFQTTSEGPVQILEISGRIEPGNGKQLSEALDALIQPGKPLK
                     VLIDLEKLDYIASAGFRELFLFGKKLSREGGALAISSLRGEVKRVFELAGFATAYPVR
                     ETREAGLAHFGN"
     gene            complement(2308..2781)
                     /locus_tag="VIS74_08265"
     CDS             complement(2308..2781)
                     /locus_tag="VIS74_08265"
                     /inference="COORDINATES: protein motif:HMM:TIGR00413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HEY5793278.1"
                     /translation="MRLIALLLLLGMAVLGLGGCGTPSYRGYKYKPYKVRGVTYYPLA
                     PSEALGYVETGTASHYQVGSYLFPGRTAIGEKMTPSTECAAHKTLPLPCRIRVTNLAN
                     GRSLVVRVNDRGPFVGGRMLDVSGKVAKKLDFHRAGLTRVRIEVLSVGDGRHRLR"
     gene            complement(2843..3436)
                     /locus_tag="VIS74_08270"
     CDS             complement(2843..3436)
                     /locus_tag="VIS74_08270"
                     /inference="COORDINATES: protein motif:HMM:NF019896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase family
                     metallohydrolase"
                     /protein_id="HEY5793279.1"
                     /translation="MNRRQAIGLFASAGLGLLVSSSPAHAFFEFFRSAPSADDFLQQL
                     KLPEEWLVQLGPNLGAYALFLNRLSLRRISIKQIVAAHAKKHGRVANMIPPKALWGNI
                     RKTLKVVDQLSVRMGEPVTEVVSMYRCPSYNATCPGAKRNSYHLVNNAMDVRMDCPPR
                     KVAAMAREMRAANLFQGGVGRYSGFTHIDTRGKNADW"
     gene            3697..4023
                     /locus_tag="VIS74_08275"
     CDS             3697..4023
                     /locus_tag="VIS74_08275"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEY5793280.1"
                     /translation="MSSFFIPLKGRLDVTSSQALQTGLERMLDSLDPAPRHLILGLEK
                     VTFLGSAGLRLLLVLHKRCLAQGGSLVLSGVRHPAHEVLQMAGLDDVFPRYAHETEAR
                     RALDGK"
BASE COUNT          793 a         1276 c         1259 g          785 t
ORIGIN      
        1 atgatcagca ccaggcgttt cggcgtctcc ggcggcaggg ccagagccgg gctggcggcc
       61 gcgggctctc ccacgcgcag ggggagatcg acggtaaagg cgcttccctg cccggccagg
      121 ctttcgaccg agatgtctcc gcccatcatc tggcaaaatt tccggctgat cacgaggccg
      181 aggccggtgc cgccgtattt gcgcgtggtg gagtcgtcgg cctgggtgaa ggcctggaag
      241 aggcgcccca attgcccggg ggtcatgccg atgccggtgt cggccacgcg aatttggatc
      301 cacggggaac cctgctggga aaaggttttc gcggagaggg tgatcaggcc cttttccgtg
      361 aatttggagg cgttgctgag cagattaaac agcgtctggc ggaccttggt cagatcggac
      421 ttcatcgtgc ccaggccggc gtctttttgg agatcgagct tgttttgatt tttgtcgagc
      481 agcggctgca cggtcgaaac cacctcgccg agcatttggt ccacggaaaa ttcctcgagg
      541 tagagcgtca ttttcccggc ctcgattttg gaaaggtcga gcacgtcatt gatgagcgcg
      601 aggaggtgtt tgccggcgga gcggattttt tgcagatcgg caatggcggc atcgttcccg
      661 ccatcctccg cgtcctcgat gagcatctcg ctgtagccga tgatggcatt catcggcgtg
      721 cgcaattcat ggctcatgtt cgccagaaaa gcgctcttgg tgcggttcgc cgactcagcg
      781 gcgtcgcggg ccaggagggc ttcctggcgg gcggtttccg tcagggccag cgcggcggag
      841 aggcgcgacg cgagccagaa ggaggcggcg acgatcacca gcaggatcgc cccggtgatg
      901 ccgtaaacct gccagagaat ccgcacggtc agcgaatgga tatcgggcag ataaagcgcg
      961 gcgacgacct cgtagtccca cggcacgaat tgatggcgga ggatgctcca gtccccattc
     1021 accggcaggt gcctcgcggc gcgctcggcc acctccgcgg cgtcggcggg aatatcccgg
     1081 ctctccgtgc gaaagacgac ctcatcccgc ggattcacca gcgcaaaaaa tccgctctgg
     1141 agcagggcgc ggttgtcaat ggcccggccg atgctcgcca gcgtctcgag cccgtagccg
     1201 acataataaa tgccgatcac ctccccgccg gcggaataca tcggctcgta accggtcaga
     1261 tacggcttgc cgaggatgtc caccacgcca aagaaggatt cattccggcg gatggcggca
     1321 atggcgcggc cattcggatc cagctcggtg ccgaccgcgc gggtgccgtc gtcgcgccgg
     1381 acattggtgg agacacggat aaagcgatcc ccctgcttca caaaaagcgt ggcggtgccg
     1441 cccatgagac gcttgacgcg gtccacttcc tcgaaggagc tgtcaatggc cttatcgcca
     1501 aaataaagcc gcccctccac caggcgcggc tcccccagct tgcgcgcctc cattttcaac
     1561 acttccaggg aggcgcggac gagattggaa tagacggtgt cggtcgcctc aaggacgtcg
     1621 atgacgtgat cgagcttcga gtccatctcc acgcgcgcct gctggtggat gtagttggag
     1681 agctggcggt agccgatcca gccggaggcg gtcatccccg ccagaacgat cagtaaccaa
     1741 atgaaaacct tggtgcgaag ggagcggtgc atggcggcga ttgggaggca atggtaagat
     1801 tgctgttgcc cccgacaagc cttttcaggt atttcggcgg acagatcccc gaatacatgg
     1861 ctgaatttca aaccacttcc gaaggcccgg tgcaaatcct ggaaatttcc gggcggattg
     1921 agccaggcaa tggcaagcaa ctcagcgaag cgctcgacgc gctcatccag ccgggcaaac
     1981 cgctgaaggt gttgatcgat ctggaaaaac tcgactacat cgccagcgcg ggtttccggg
     2041 agctgttcct tttcggcaaa aagctttccc gcgaaggcgg cgccctggcc atcagctcgc
     2101 tgcgcggtga ggtcaaacgg gtattcgaac tggccgggtt tgccactgcc tatccggtgc
     2161 gggaaacccg cgaggccggt ttggcgcatt tcgggaatta accgaaaccc gccgcggcaa
     2221 ccggcctcat tatattcagc gttccccaga gccccttcga ttcagtttgt cgccggggga
     2281 aatgcgttca attaacccga aaagacccta acgcagccgg tggcgcccgt cgcccacgct
     2341 caggacctcg atccgcaccc gcgtgagccc cgcgcggtgg aaatcgagct ttttggcgac
     2401 cttgccgctt acatcgagca tgcggccgcc cacaaacggc ccgcgatcat tcacccgcac
     2461 caccagggac cggccgttgg cgaggttcgt cacgcgaatc cggcagggca acggcagggt
     2521 tttgtgcgcc gcgcattcgg tggagggcgt cattttttcc ccgatggccg tgcggcccgg
     2581 aaacagatac gaaccgacct ggtaatgcga cgcggtcccc gtctccacgt aacccagcgc
     2641 ctcgctcgga gccaggggat aataggtcac gccgcgaacc ttgtagggtt tgtatttgta
     2701 gccccggtag ctcggagtgc cgcatccacc cagccccaga acggccattc ccaacaggag
     2761 aaggagcgca atcaatcgca tgggccccgg atagcggctt tcccccttcc cgtcgagccg
     2821 gaatgcggac gaatgaagcg gcctaccagt ccgcgttctt cccgcgggtg tcgatgtgcg
     2881 tgaagcccga ataacggccc acgccgccct gaaagaggtt ggcggcgcgc atttcccggg
     2941 ccatggccgc caccttgcgc ggcgggcagt ccatgcgcac atccatggcg ttgtttacca
     3001 gatggtagga gttccgcttc gcccccgggc aggtggcgtt gtagctcggg cagcgataca
     3061 tcgaaaccac ttcggtcacc ggctccccca tccgcacgga taattgatcc acgaccttca
     3121 gggtcttgcg gatgttgccc cacagggcct tcggcggaat catgttcgcc acccgcccgt
     3181 gctttttggc atgggcggca acgatttgtt tgatggaaat ccggcggagg ctcaggcggt
     3241 tgagaaaaag ggcgtaagcg cccaggttcg gcccgagctg gaccagccat tcctcgggca
     3301 gcttcaattg ctggagaaaa tcgtccgccg aaggcgcgga gcggaagaat tcaaaaaaag
     3361 cgtgggcggg agaactggag accagcaatc ccaaaccggc cgaggcgaag aggccgatcg
     3421 cctggcgccg attcattccc ggcaggcttg aacgattttt gtgattttcc gatgggttct
     3481 cttccaaaat gcgcgctcct ttttgagtta aaaaaggggg cttactacag ccatccgggg
     3541 gcatcgtcaa gcaactttcg tgccaaaaac gggcgcatca cgcagcaacc caaacgaagc
     3601 gtgacggttg cgtttccgcg ttttttcatt caaatttttt ccggcagcgg catccatcgc
     3661 ctggctgcca aattctcccc atttccccaa attcccatga gttcattttt cattcccctg
     3721 aaaggccggc tcgatgtcac ctcctcccag gcgctgcaga ccggcctcga acgaatgctg
     3781 gattcgctcg accccgcgcc ccgccacctc attctgggcc tcgaaaaggt cacctttctg
     3841 ggcagcgccg ggctccgcct gctgctggtt ctgcacaaac gctgccttgc ccagggcggc
     3901 tccctggttc tgagcggcgt gcgccatccc gcccacgaag tgctgcagat ggcgggactg
     3961 gacgacgttt ttccgcgcta cgcccacgaa accgaagccc gccgggcgct ggacggaaaa
     4021 taacggctac cgcgacaaat ccgcctgcgt gagggtctta aatccctgtc cctgcaggac
     4081 cgcgctggcg cattccatgt cgtccacgtg cat
//
LOCUS       DATDAY010000418         2086 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_824722, whole genome shotgun sequence.
ACCESSION   DATDAY010000418 DATDAY010000000
VERSION     DATDAY010000418.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2086)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2086)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2086
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_824722"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..813)
                     /locus_tag="VIS74_08280"
     CDS             complement(<1..813)
                     /locus_tag="VIS74_08280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5793281.1"
                     /translation="MTTEKIRLSVIIPLEFHRGLAERCIAAWSRGQTYPRECYQIVIV
                     APDTCSEKQLRAIRAALHPADRLLVKPLHHDMPLVAAGAAEAEGAAFFFTEAHCLPEP
                     DTLEQAAQILAERPDWAGFSGRSIPITHNLLSEIEAEMYDAHIRENLLNHSWLKVLDQ
                     CLVLRREPYFSCGGLEPEYGHFAEWVIAARLHHGGQVVGYDARAAIRHYYIGELHELE
                     EFTEDFSGGEMRFHARHRHGPWSRLLPVPEELTRAGSLCPEPAPVMLRLLVRD"
     gene            complement(852..1520)
                     /gene="trmD"
                     /locus_tag="VIS74_08285"
     CDS             complement(852..1520)
                     /gene="trmD"
                     /locus_tag="VIS74_08285"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEY5793282.1"
                     /translation="MKIQILTLFPEICRAALGESMMKRAQAKGAALIEPLNLRDWAPG
                     KHHVTDEPPYGGGPGMVMKIEPIHRALEELRTPVAKVILMTPQGRRFNQSLAESYARE
                     AHLIVLCGHYEGVDQRVADHLVDDEISIGDYVLTNGAISAVVFVDAVVRLLPGVLGDE
                     QSAQRESFSTGLLDYPHYTRPETFRGWSVPPILLSGNHAAIEKWRQEEALRLTRERRP
                     DLLG"
     gene            1638..>2086
                     /locus_tag="VIS74_08290"
     CDS             1638..>2086
                     /locus_tag="VIS74_08290"
                     /inference="COORDINATES: protein motif:HMM:NF013106.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HEY5793283.1"
                     /translation="MARPKKRRSTPPRPTAPPPKPPPRKRSLLWRFVEWGFKLALALA
                     VVWGLIGGFYYLLSLRYNLREMGTMPQRSAVFDRNGEFYSRLSGENRVVVPFDQVSNH
                     FINALICREDARFYQHVGVDPIGIARAVVRNLLMGGVRQGASTITQQL"
BASE COUNT          382 a          670 c          641 g          393 t
ORIGIN      
        1 atcccggacc agcagccgga gcatgaccgg agccggctca gggcacaaac taccggcgcg
       61 agtcaattcc tcggggacgg gaagcagccg gctccagggc ccgtggcggt ggcgcgcatg
      121 gaagcgcatc tccccgcccg aaaagtcctc ggtgaactcc tcgagttcgt ggagttcgcc
      181 aatgtaataa tggcggatcg ccgcgcgcgc atcgtatccg accacttgcc cgccgtgatg
      241 cagccgcgcg gcgatcaccc attcggcaaa gtggccgtat tccggctcca ggccgccgca
      301 ggaaaaatag ggctcgcggc gcaggacgag gcattggtcg aggaccttca accaggagtg
      361 gttgagcaga ttttcccgga tatgcgcgtc atacatctcc gcctcgatct cggagagcag
      421 gttgtgggta atgggaatgg accgcccgct gaaacccgcc cagtccggac gctccgccag
      481 gatttgggcg gcctgttcca gcgtgtccgg ctcgggcagg cagtgagctt cggtaaaaaa
      541 gaaggccgct ccctccgcct ccgccgcgcc ggccgccacg aggggcatgt cgtggtgaag
      601 gggcttgacg agcagccggt cggcgggatg aagcgcggcg cggatcgccc gcagctgctt
      661 ctccgagcag gtgtcgggag ccacgatcac gatctggtag cattccctcg gatacgtctg
      721 cccccgcgac caggcggcaa tgcagcgctc ggcgagcccc cggtgaaact ccaggggaat
      781 aatgactgaa agccggattt tttctgtcgt catggcaatt ccttattccg caacatcgag
      841 tcaaaaccgc tctaaccgag caaatccggc cgccgctcgc gggtcagccg aagcgcttcc
      901 tcctgccgcc atttctcgat ggccgcatgg ttgccggata gcagaatggg cggcacgctc
      961 cagccccgaa acgtctccgg ccgggtgtag tgcggataat ccaaaagccc ggtggaaaag
     1021 gattcccgct gcgcggactg ctcgtcgccc agcacgccgg gaagcaggcg caccaccgcg
     1081 tccacgaaca ccacggcgga gattgcgcca ttcgtcagca cgtagtcgcc gatggaaatt
     1141 tcgtcatcca ccaggtgatc cgccacgcgc tgatccacgc cttcgtaatg gccgcagagc
     1201 acgatgagat gcgcttcccg cgcgtagctt tcggcgaggg attgattgaa gcgccttccc
     1261 tgcggcgtca tcaggatgac cttcgccacc ggcgtgcgca gctcctccag cgcccggtgg
     1321 atcggctcga tcttcatcac cattcccggc cccccgccgt agggcggctc gtcggtgacg
     1381 tggtgtttgc ccggcgccca gtcccgcaga ttgagtggct cgatgagagc cgcccccttg
     1441 gcctgcgcgc gcttcatcat gctttcgccc agcgcggcgc ggcagatttc gggaaacagg
     1501 gtaaggattt ggatcttcac ggattcattt tcttaccatg aaggacagga aggacatgaa
     1561 gacaaattat tggttccgaa aatccttcat gttcttcatg cccttcatgg tttaattccg
     1621 tttcagcaaa tccatccatg gcgcgcccca aaaaacgtcg ttcaactccg ccgcggccga
     1681 ccgcgccgcc gccgaaaccg ccgccgcgca agcgatccct gctttggcgg ttcgtcgaat
     1741 ggggtttcaa actcgccctg gcgctggccg tggtctgggg gctgatcggc ggcttttatt
     1801 acctgctttc gctgcgctac aacctccggg aaatgggaac gatgccgcag cgttcggcgg
     1861 tgtttgaccg gaacggcgaa ttttattccc gcctctcggg cgaaaatcgc gtggtcgtcc
     1921 cgttcgacca ggtctccaat catttcatca atgcgctgat ctgccgcgag gacgcgcggt
     1981 tttaccaaca cgtcggggtg gatcccatcg gcatcgcgcg ggccgtggtt cggaatctcc
     2041 tcatgggcgg cgtgcggcag ggggccagca cgatcaccca gcagct
//
LOCUS       DATDAY010000419         3222 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_68596, whole genome shotgun sequence.
ACCESSION   DATDAY010000419 DATDAY010000000
VERSION     DATDAY010000419.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 3222)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3222)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3222
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_68596"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..411
                     /gene="nusB"
                     /locus_tag="VIS74_08295"
     CDS             <1..411
                     /gene="nusB"
                     /locus_tag="VIS74_08295"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEY5793284.1"
                     /translation="EAAMQYLYLRDLNSAEGNGQLDDFYAIRSLSPSARRFCADLVKG
                     ILTHAPEIDESIKKYAVNYELHRISAVDRNILRMAIYEMLHCTDIPPVVSINEAIEIA
                     KKYSAEDSGKFVNGVLDRIRLSLPRDARNPQAAR"
     gene            412..1260
                     /gene="ftsY"
                     /locus_tag="VIS74_08300"
     CDS             412..1260
                     /gene="ftsY"
                     /locus_tag="VIS74_08300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEY5793285.1"
                     /translation="MALGFFKSIVARFTGKPVDPEELEETLIRADLGVPLSLGILEKI
                     REANGFANTDTAERLIKESLLGLFPPGTPPLRPLPARPKVILLVGVNGTGKTTSAAKL
                     AHYLKTRRHTVLLAAADTFRAAAIEQLVAWGSRLNLEVIKGQYNGDPAALCYDAFVSA
                     HRKNTEFLICDTAGRLHTKDNLMQELTKISRTLAKHSPDCPDEKWLVVDATTGSNGLA
                     QAKEFHQAVGLTGIILTKLDGSGKGGNAAAIQSELGISPRFIGTGERPEDFAVFEREA
                     FVNRLV"
     gene            complement(1272..2150)
                     /locus_tag="VIS74_08305"
     CDS             complement(1272..2150)
                     /locus_tag="VIS74_08305"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HEY5793286.1"
                     /translation="MDVRFFQLLPLAVIFAACTSVPEPVAVRQAIPSQPVALAPVGAP
                     AVWPAEAPAIYAQSAILIDAHSGRVLYQKNADIPRPVASTQKLLTALVVARDGDLDQI
                     VQVARADTQVEPTKLGLRTGERYSRRHLLEAMLVKSCNDTAAALARDHSGSVPAFSAE
                     MNQLAAQIGATSSHFANPHGLPASQYSTARDMARIAYRAYRIPALRVIMARPVQPFVF
                     NNGRVRYLENTNKLLKRSSLYTGMKTGYTDAAGRCLVTSASNGWRDLILVQLGSKTRY
                     IFDDAERLIASAYSFR"
     gene            complement(2329..2661)
                     /locus_tag="VIS74_08310"
     CDS             complement(2329..2661)
                     /locus_tag="VIS74_08310"
                     /inference="COORDINATES: protein motif:HMM:NF037832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5615 family PIN-like protein"
                     /protein_id="HEY5793287.1"
                     /translation="MKLLFDENLSPRLVSLLADLFPHSAHAHDLSLGQTDDAIIWQYA
                     KRHNYTLVSKDSDHYELSMLYGHPPKVIWIRIGNCGTKSIEEQMRLHHLAISAFILDP
                     DQSAFLLP"
     gene            complement(2658..2906)
                     /locus_tag="VIS74_08315"
     CDS             complement(2658..2906)
                     /locus_tag="VIS74_08315"
                     /inference="COORDINATES: protein motif:HMM:NF016167.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HEY5793288.1"
                     /translation="MNQYFSSVDYRKLITIEPGKRSGKPCIRGLRITVYDVLDYLASG
                     MSHEELLADFPYLTEEDIRACLAFAADSGRRTAYAVAA"
BASE COUNT          722 a          976 c          935 g          589 t
ORIGIN      
        1 gaagctgcca tgcaatatct ttatctccgc gatctcaaca gcgcggaagg aaacggccag
       61 ctggatgact tttacgccat ccgcagcctc agccccagcg cgcggcggtt ttgcgccgac
      121 ctggtgaagg gcatcctcac gcatgcgccc gagatcgatg aatccatcaa aaaatatgcg
      181 gtgaactacg agttgcaccg catctccgcg gtggatcgca acatcctgcg catggccatc
      241 tacgagatgc tgcactgcac ggacattccg ccggtggttt ccatcaatga agccatcgaa
      301 atcgccaaga aatacagcgc ggaggattcc gggaaattcg tcaacggcgt gctggaccgc
      361 atccgcctct cgctgcctcg cgacgcccgg aacccgcagg ccgcccgcta aatggccctc
      421 gggtttttca aatccatcgt cgcccgcttc accgggaagc ccgtcgatcc cgaggagttg
      481 gaggagaccc tcatccgggc cgatctcggc gtgccgctga gcctcggcat cctcgaaaaa
      541 atccgcgagg ccaatggatt tgccaacacg gacaccgccg agcgcctgat caaggagtcg
      601 ctgctcgggc tcttcccgcc cggcaccccg ccgctgcgcc ccctgcccgc gcggccgaaa
      661 gtcatcctgc tcgtcggcgt gaacggcacc ggcaaaacca cgtccgccgc caagctcgcc
      721 cattacctga aaacccggcg gcatacggtc ctgctcgccg ccgccgacac gttccgcgcc
      781 gcggccatcg aacagctcgt cgcgtgggga agccgcctga acctcgaagt catcaagggc
      841 caatacaacg gcgaccccgc cgcgctgtgt tacgacgcct tcgtcagcgc gcaccgcaag
      901 aacaccgaat tcctcatctg cgacaccgcc ggccggctgc acacgaagga taacctgatg
      961 caggaactca ccaagatttc ccgcacgctg gccaaacatt cgcccgattg cccggatgaa
     1021 aaatggctcg tcgtggacgc caccaccggc agcaacggcc tcgcgcaggc gaaggaattt
     1081 caccaggccg tcggtctgac cgggatcatt ctcaccaagc tcgacggcag cggcaagggc
     1141 ggcaacgcgg ccgccattca atccgaactc ggcatcagcc cccgattcat cggcaccggc
     1201 gaacgcccgg aagactttgc cgtgttcgag cgcgaggcat ttgtgaaccg gctcgtttga
     1261 gccgccgtca ttcagcggaa tgaataagcg gacgcgatca ggcgttcggc gtcgtcaaag
     1321 atgtatcgag tcttgctgcc gagttgaacc agaatgagat cccgccatcc gttggaagcg
     1381 ctggtcacca ggcaccggcc ggccgcgtcg gtatagccgg ttttcatgcc ggtgtagagg
     1441 gaggagcgtt tcaggagctt attggtgttt tccaggtagc gcacccggcc attgttaaag
     1501 acaaagggct gcaccggccg ggccatgatc acccgcaggg cgggaatgcg atacgcgcga
     1561 tacgcgatcc gggccatgtc gcgcgcggtg gaatactggc ttgccggcag gccatgggga
     1621 ttggcgaaat gggaactcgt ggctccgatc tgggcggcca gctggttcat ctcggccgag
     1681 aacgccggga cgctgccgct atggtcgcgg gccaaggcgg ccgcggtgtc gttgcagctc
     1741 ttgaccagca ttgcctcgag cagatggcgc cgggaataac gctcgcccgt ccggagaccc
     1801 agcttggtcg gctccacctg cgtgtccgcc cgcgcaacct gcacgatctg gtccagatcg
     1861 ccatcccgcg cgaccaccag cgccgtcagc agtttctgcg tgctggccac ggggcgcgga
     1921 atgtcggcat ttttttgata cagcacccgc ccgctatgcg catcgatgag aatggccgac
     1981 tgggcataaa tggccggcgc ctccgcgggc cagacggccg gagcccccac cggcgcgagc
     2041 gcgacgggct gggacggaat cgcctgccgg acggcaaccg gctcgggaac cgaagtgcag
     2101 gcggcaaaaa taacggcgag tggcaggagt tggaaaaaac gcacgtccac gaaaagcaat
     2161 ctttgttcca atctgtcaat caattcaccg ttcgaccttc cgaaaaggaa aacgcgacac
     2221 actcgcggaa ggcgggatta tttatggaat ccaaaatcga ttatcccgaa agtgccatcg
     2281 gccggttaat ggaggagccc tgcgcggttt tccgcccgga aatcatccct agggcaacaa
     2341 aaatgccgac tgatctggat cgaggataaa tgcggaaata gcgaggtgat gcaaccgcat
     2401 ctgctcttca atagactttg tgccgcaatt tccaatccga atccaaatta cttttggagg
     2461 atgtccataa agcatgctga gttcgtaatg atctgaatct tttgagacca atgtataatt
     2521 atgcctcttc gcatattgcc atataatcgc atcatcagtt tgacctaatg ataaatcatg
     2581 ggcatgcgct gaatgaggaa ataaatccgc taaaagggaa actaatcttg gagaaagatt
     2641 ctcgtcgaaa agaagtttca agcagcaacc gcgtaggcag tcctccggcc agaatcagcg
     2701 gcaaatgcca ggcaggctct aatatcttct tcagtcaagt atgggaaatc agccaaaagc
     2761 tcctcgtgag acattcctga tgccaagtaa tcaagcacat cgtaaacggt gatgcggagc
     2821 ccgcggatgc agggcttgcc actccgtttc cctggctcaa tagtgatgag cttgcggtaa
     2881 tccacagagg aaaaatactg gttcatggcg tctgagtcaa ccgcgcacaa tttagggccg
     2941 gacgccagcc atcccgaacg gatccgcaag ctctggagtg atgcgaatgg agggagtatg
     3001 ggacatggac atagtcggtc cgtccgtcaa catccggggg tgtaaaagtt cttctgtaat
     3061 tggtctttcc ctctgagcgc cacatccggg ggcggcggag agaaggcgag cgtagcgagc
     3121 cgaacgcaga cggccccgga tggggcgctg cgcgaagttc gccggaggct ggccagtgtg
     3181 gtttcctgtt ggggtgtagc caaacacatc caacaaaaac gc
//
LOCUS       DATDAY010000420         2768 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_1597538, whole genome shotgun sequence.
ACCESSION   DATDAY010000420 DATDAY010000000
VERSION     DATDAY010000420.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2768)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2768)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2768
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1597538"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            97..1188
                     /locus_tag="VIS74_08320"
     CDS             97..1188
                     /locus_tag="VIS74_08320"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HEY5793289.1"
                     /translation="MSKIRFQVLSPLFPENPAPRVTGSLPALGNWNPAEGLELKPHGI
                     WREAEFATEEGEHFEYKITRGSWQTEAVDAWGDVLPNRSHEVWGDATLHVTVADWKDT
                     HAGRLTRERVGSRILADSREILIWLPPSYALDPERRFPVIYLHDGDNLFDPQTSPITG
                     VDWAADEWVRLLARTGGVPESIVAGVCHPDGFNQENIPLRDVDLSPELGGPAYAAFVA
                     SELAPYLDTHYRTDASPLRRTLGGADLGALNAFYTAIHHPGVFSRFLCLSTRFGDLSD
                     SPAENSRELAALECLPGLPRENRFYFDYGTRGLDAGLGPFHERLGAILREKGARDGAE
                     FQIARVEGGGHDEASWRQRFGPALRFLAQ"
     gene            1363..1569
                     /locus_tag="VIS74_08325"
     CDS             1363..1569
                     /locus_tag="VIS74_08325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraY domain-containing protein"
                     /protein_id="HEY5793290.1"
                     /translation="MLAIRLDPDIEKRLARLAERTGRTKTFYAREAILEHLEDLEDIY
                     LAEQRLRRPTATYSAQNVKRELGL"
     gene            1553..1819
                     /locus_tag="VIS74_08330"
     CDS             1553..1819
                     /locus_tag="VIS74_08330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEY5793291.1"
                     /translation="MNWVYRFDERAVKELKKLGVQAQRDILTYLDERVASDSDPRRFG
                     KGLKADLAGLWRYRVGDYRILCQIRDRELLVLVVAAGHRKNIYE"
     gene            complement(1895..2476)
                     /locus_tag="VIS74_08335"
     CDS             complement(1895..2476)
                     /locus_tag="VIS74_08335"
                     /inference="COORDINATES: protein motif:HMM:NF042461.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6580 family putative transport protein"
                     /protein_id="HEY5793292.1"
                     /translation="MKKEGLIFTVLLLIFGAFYRVIRLELLPELPNFSPAMAIAFCGA
                     LFLPGRMVFAVSLPALFISDLLLNVHYGQSLLHAEMLSSYFCYGAAIALGLLLRGKGP
                     GWIFGATLFNAVLFYLVTNTVSWATSPLYAHDFGGWLQSITVGLPGFPPSLVFFRNSL
                     VSDLLFTALFLGVGYAAKNRSAGPNLCRQEASS"
     gene            complement(2584..>2768)
                     /locus_tag="VIS74_08340"
     CDS             complement(2584..>2768)
                     /locus_tag="VIS74_08340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5793293.1"
                     /translation="FYGIDQNEITVHFRHHGQAMVAFASGSVGFLPIDETTRDSRAPK
                     ASIGRFAPRGDKKYLE"
BASE COUNT          557 a          784 c          906 g          521 t
ORIGIN      
        1 accacagaga acaccgaggg cacagagatt tgtggattta attccgaaac cccgtgttcg
       61 cggcgcccgc tgtggtttat attcttacgt atccccgtga gcaaaatccg ttttcaggtt
      121 ctctcgccgt tgttccccga aaatcccgcg ccgcgggtca ccggaagcct gcccgcgctg
      181 gggaattgga atcccgcgga gggactggaa ctcaagccgc acggcatctg gcgggaagcg
      241 gagtttgcca cggaggaggg cgagcatttt gaatacaaaa tcacccgcgg ctcgtggcag
      301 acggaggcgg tggacgcgtg gggcgacgtg ctgccgaacc ggtcgcacga ggtctgggga
      361 gatgccacgc tgcatgtgac ggtcgccgat tggaaggaca cgcatgccgg acggctgacg
      421 cgggaacggg tcggctcgcg catcctggcc gactcgcgcg aaatcctgat ctggctgccg
      481 ccctcctacg cgctcgatcc ggagcggcgg tttccggtca tttacctgca cgatggcgac
      541 aatctcttcg atccgcagac gagcccgatc accggcgtgg attgggcggc ggatgaatgg
      601 gtgcggcttc tggcccggac gggcggggtg ccggaatcca tcgtggccgg cgtgtgccat
      661 ccggacggct tcaaccagga aaacatcccg ctgcgggacg tggacctttc gcccgagctg
      721 ggcggaccgg cctacgcggc gttcgtggcg agcgaactgg cgccatatct ggacacgcat
      781 taccggaccg acgcctcgcc cctgcgccgg acgctgggcg gggcggatct cggcgcgttg
      841 aatgcgttct ataccgccat tcaccatccg ggcgttttca gccggttcct ttgcctttcg
      901 acgcggttcg gggatctgtc ggattcgccc gcggaaaatt cgcgggaatt ggcggccctg
      961 gaatgcctgc cggggctgcc gcgggagaat cgtttttatt tcgactacgg cacgcgcggg
     1021 ctcgatgccg ggctggggcc gttccacgag cggctcggcg cgattttacg cgaaaagggc
     1081 gcgcgcgacg gcgcggaatt tcagatcgcc cgcgtcgagg gcggcggtca cgacgaggct
     1141 tcctggcggc agagattcgg cccggcgctg cggtttttgg cccaataaaa acccaacgcc
     1201 ctgagtcatt ctctggttat tttctaccat gaagatcatg aaggttcctc tcttcaaacc
     1261 cttcatgtcc ttcatggtaa aattattccg acgttctggg ttgtgcgcta aatggaaaat
     1321 tacccgcttg ctatatacgt atagcggatc tagtctcagc tcatgctcgc catccgcctt
     1381 gatcccgaca tcgagaagcg ccttgcccgc ctggccgaac ggaccggacg gaccaaaacc
     1441 ttttatgccc gcgaagcgat tctggaacat cttgaagacc tggaagatat ctatctggcc
     1501 gagcagcggc ttcgccgtcc gacggcgacg tattctgccc agaatgtgaa gcgtgaactg
     1561 ggtttatagg ttcgacgaaa gggcggtcaa ggagctgaaa aaactggggg tgcaggccca
     1621 gcgggacatc ctcacttatc tcgacgaaag ggtggcaagc gatagtgatc cgcgccgctt
     1681 cggcaaaggg ctcaaggcgg acttggcggg gctttggcgc taccgagtgg gcgactatcg
     1741 cattctttgc cagatcagag atcgggaatt gctcgtgctg gtggtcgcgg caggccatcg
     1801 caaaaatatt tacgagtgag ccaatccgca gtctttcgaa atcccgcccg atgagcggcg
     1861 cggaatttca gatcgcccgc gtcgagggcg gcggtcacga cgaggcttcc tggcggcaga
     1921 gattcggccc ggcgctgcgg tttttggcgg cgtagccgac cccgaggaaa agcgcggtga
     1981 agagcaggtc gctcaccagc gaattgcgga aaaacacgag ggacggcggg aagccgggca
     2041 ggccgacggt gatgctttgc agccagccgc cgaaatcgtg cgcatagagc gggctggtcg
     2101 cccaggaaac cgtgttggtg acgagataaa acagcaccgc gttgaaaagc gtcgccccga
     2161 aaatccagcc gggccccttg ccgcgcagga gcagtccgag ggcgatggcg gcgccgtagc
     2221 agaaatagct ggagagcatt tccgcgtgca gcagcgactg gccgtagtgg acgttcaaga
     2281 gcaaatcgga aatgaacagc gcgggcaggg aaacggcgaa aaccatccgc cccggcagga
     2341 aaagcgcgcc gcagaaggcg atcgccatgg ccggagagaa gtttggcaat tcgggcagaa
     2401 gctccaggcg gatgacgcga taaaacgcgc cgaagatcag gagcagcacg gtgaaaatga
     2461 ggccttcttt tttcatgtgg tgaaagtcgg cgggctcggc gatcccgccc tacccgggga
     2521 ttggtagggc agggttggca accctgcctt tggattagaa tgattaaact caatttgcgg
     2581 ggtttattcc agatactttt tgtccccccg cggggcaaag cggccgatgg atgccttcgg
     2641 cgcacgggaa tcccgggtgg tttcatcgat tggcagaaaa cccacggaac cgctcgcgaa
     2701 ggcgaccatc gcctggccgt gatggcggaa gtgcacggtg atctcgtttt gatcaatgcc
     2761 atagaact
//
LOCUS       DATDAY010000421         2137 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_246616, whole genome shotgun sequence.
ACCESSION   DATDAY010000421 DATDAY010000000
VERSION     DATDAY010000421.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2137)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2137)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2137
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_246616"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..644)
                     /locus_tag="VIS74_08345"
     CDS             complement(<1..644)
                     /locus_tag="VIS74_08345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5793294.1"
                     /translation="MNPEETPSTSNVRAPADEASRHQSELFSGDAFASRTARFRAPAR
                     KPQPARKPEPGLDETIREVLDRELKAPPVPEPPSEAGWLPARMLNEYVYCPRLFFYEH
                     IDGIFVHNADTERGKDIHTRVDKGSGNLPSPAPADGETPEAANPEGEIIHSRSVQMGS
                     ARLGVTAKLDLVESSGAGGEVSPVDYKAGSPREGAEAHELWPADRMQLGLQMLIL"
     gene            complement(1024..1245)
                     /locus_tag="VIS74_08350"
     CDS             complement(1024..1245)
                     /locus_tag="VIS74_08350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5793295.1"
                     /translation="MTGTKAPDIDWSQMTWKGSRLLQHREHLALPFRRKIELIEEMCD
                     QGRRSIEARKRRGLPYIDPYTDQVVRSGQ"
     gene            complement(1223..1750)
                     /locus_tag="VIS74_08355"
     CDS             complement(1223..1750)
                     /locus_tag="VIS74_08355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5793296.1"
                     /translation="MKLSSFETIVRALNDAGVRYLVVGGLAVNAHGYGRNTYDVDLVV
                     SLDAENIRRAFSALKEAGFAPVQPITAEQFGDRALRESWRKDKGMLVLKIWSDRHRET
                     PVDIFVYEPFDFSEEYDAAAWQELAPDLKVPVIRLEALLKMKREAGRPKDLADLDELS
                     LLYKKPSSYDRDQSA"
     gene            complement(1784..>2137)
                     /locus_tag="VIS74_08360"
     CDS             complement(1784..>2137)
                     /locus_tag="VIS74_08360"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEY5793297.1"
                     /translation="FLWMLGDPGRLSPQAAAAIQNPERSVFVSAVTAVEIAIKRRLGK
                     LEAPEGLDAEIASRGLLELPLKFRHGERLLTLSDHHQDPFDRMLIAQAMEEDLTLITH
                     DRKMEPYGGIKILWS"
BASE COUNT          422 a          612 c          686 g          417 t
ORIGIN      
        1 aggatgagca tttgcaggcc gagttgcatg cggtcggccg gccagagctc atgggcctcc
       61 gcgccctcgc ggggcgagcc cgccttgtag tccacgggcg agacctcgcc gcccgcgccg
      121 ctggattcca cgagatcgag cttcgccgtg acgccgagcc gggccgatcc catctgcacg
      181 ctgcgcgaat ggatgatctc gccctccgga ttcgcggctt ccggggtctc gccatcggcg
      241 ggcgccgggg aagggagatt gccggagccc ttgtccacgc gggtatggat gtccttgccg
      301 cgttcggtgt cggcgttgtg gacgaagatt ccgtcgatgt gttcgtaaaa aaacagccgc
      361 gggcaataga catactcgtt gagcatgcgc gcgggcagcc agccggcttc gctcggcggt
      421 tcggggaccg gcggggcctt gagttcccgg tcgagcactt cgcgaatggt ctcatcgagt
      481 ccgggctccg gtttcctggc gggttggggt ttgcgcgcgg gggcgcggaa acgagcggtc
      541 cgggaggcaa aagcgtcgcc ggaaaagagc tccgattgat gccgggaggc ctcgtcggct
      601 ggtgcgcgaa cgtttgacgt gctgggggtt tcctcgggat tcatggccgg gaagtcagag
      661 gataaaccga tttcccggaa aggatcaata ctggaaaaat tgtccctgat gctggcgcaa
      721 gaccaagttc acggtcaaat cggtggaatg cgaaagtgac gcaagcttcc agcttgcgaa
      781 gtgaagcgtt tcccgatggc aagctggaag cttgccccgc cttttgacga acctttcaga
      841 ggccgcgggc tttctgaaag gcccgccgcg gatggcaaaa attgccgcga aagggcgcga
      901 gaaaacgcga ggaatttttg accacagcag ccggccgcag ggagacggaa tgccgaaggc
      961 ggccccgaag gggtgagcaa aacgaaccaa atggaacgaa tgcgcaaggg tattaaaagc
     1021 cggtcactgg ccggaccgga ccacttgatc cgtgtaggga tcaatgtaag gcaaacccct
     1081 gcgtttgcgc gcttcgatgg agcgacgacc ctggtcgcac atttcctcga tcaactcgat
     1141 tttgcgtcgg aagggcaggg caaggtgttc gcggtgctgg agcaggcggc tgcctttcca
     1201 ggtcatctgg ctccagtcga tgtcaggcgc tttggtcccg gtcataactt gatggttttt
     1261 tgtacagtaa actcaattcg tcgagatccg caagatcctt gggccggccg gcttcgcgtt
     1321 tcattttcag cagcgcttcc agccggatca ccggcacttt caaatcagga gccagttcct
     1381 gccatgccgc cgcgtcgtat tcctccgaaa aatcgaatgg ctcatagaca aagatatcca
     1441 ccggcgtttc ccggtgccgg tcgctccaga ttttcaggac gagcatgcct ttgtctttcc
     1501 gccacgattc ccgcagggcg cgatctccga attgttcggc ggtgatgggt tgaacgggcg
     1561 caaaacccgc ttccttgagg gcggaaaagg cgcgccggat attttccgca tcgagggaga
     1621 ccacaagatc aacatcgtag gtgtttctcc cataaccatg cgcattcacc gccagcccgc
     1681 ccacgacgag atagcggaca cccgcgtcat tgagcgcacg gacgatggtc tcaaagctgg
     1741 agagtttcat gcgcagggaa cgcgggcaaa aatcgggagg cgatcaagac cagaggattt
     1801 tgatcccgcc atagggctcc attttccggt cgtgagtgat caaggttaaa tcctcctcca
     1861 tcgcctgcgc gatgagcatc cggtcgaagg gatcttgatg gtggtccgaa agcgtaagca
     1921 ggcgttcgcc gtggcggaat ttcagaggca gctcaagcag cccgcggctg gcgatttccg
     1981 cgtcgaggcc ctcgggcgcc tccagcttgc cgaggcggcg tttgatcgcg atttccaccg
     2041 ccgttaccgc gctgacaaac acggaccgtt ccggattttg aatcgccgcg gccgcctggg
     2101 gactcaggcg cccgggatca ccgagcatcc agaggaa
//
LOCUS       DATDAY010000422         3401 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_1220090, whole genome shotgun sequence.
ACCESSION   DATDAY010000422 DATDAY010000000
VERSION     DATDAY010000422.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 3401)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3401)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3401
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1220090"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..643
                     /locus_tag="VIS74_08365"
     CDS             <1..643
                     /locus_tag="VIS74_08365"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEY5793298.1"
                     /translation="DIKLELDRRAQALIESFLLEKFPAHAIFGEEGIRGDQSSEFQWI
                     IDPIDGTVNYFYGIPHFCISIALRQAGKMIVGLIYDPSRDELWTATAGGPARLNGQPI
                     RVSPRTQLSECTVSIGFSKSASAIDKGMKLFERMVRSAKKCRMQGSAALDLVYVASAR
                     LDAYIESQISLWDFAAGMLILERAGGKFQMTELADKPGKYAVVASSGNVDLGL"
     gene            678..1154
                     /gene="ispF"
                     /locus_tag="VIS74_08370"
     CDS             678..1154
                     /gene="ispF"
                     /locus_tag="VIS74_08370"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HEY5793299.1"
                     /translation="MSRSGIGYDVHRFAAERKLILGGVEIPYMMGLDGHSDADVLSHA
                     IADSLLGAAAEPDIGHLFSNTDPTIAGISSLTILSKVRDVLAAKGYRIVNVDSTVVAE
                     APKISPHLAGMKANISKALGIEPADVGVKATTNESMGFIGREEGIAALAVASIEKN"
     gene            1287..2690
                     /gene="cysS"
                     /locus_tag="VIS74_08375"
     CDS             1287..2690
                     /gene="cysS"
                     /locus_tag="VIS74_08375"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEY5793300.1"
                     /translation="MALNFFNTLTREIETFQPLDPAGRRVKLYTCGPTVYNYAHIGNF
                     RAYLFEDLLQRHLEYRGFVVDRVMNLTDVDDKTIRGCRTAGVPLGVFTKTFKDAFFAD
                     LDTLRIKRANHFPEATAANQIERMISMIATLVERGHAYQAEDRSVYFRIRSFQNYGRL
                     AHINLDELQPSGRVSSDEYEKENIGDFALWKAWDEADGDVAWDSPWGRGRPGWHIECS
                     AMATGLLGPKLDIHCGGEDNIFPHHEAEIAQSESVTGEKFVQYWLHCKHLMVEGQKMS
                     KSAGNFYTLRDLLEKGWSGREVRYLLLSVRYREPLNFTFEGLAGARAALRRLDEWGAR
                     LEQAAQGAPAAETPAGLNLDAFGAALDDDLNISAALGSLFDIVRESNRLISENQLPPA
                     QARGLLEWRDRVFSVLAFEPEAEPIPAEVAALLEQRAAARAGKDWAASDTLRDQIAAL
                     GWIVKDTKSGQQVTKKS"
     gene            2927..3103
                     /locus_tag="VIS74_08380"
     CDS             2927..3103
                     /locus_tag="VIS74_08380"
                     /inference="COORDINATES: protein motif:HMM:TIGR04256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HEY5793301.1"
                     /translation="MENKIIVDAKTVAAFTENHSAQMLGYLAMTGLELGILLNFKYAK
                     LEWTRLAKSRLLSE"
     gene            3148..>3401
                     /locus_tag="VIS74_08385"
     CDS             3148..>3401
                     /locus_tag="VIS74_08385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine diphosphorylase"
                     /protein_id="HEY5793302.1"
                     /translation="MFTPETYLSLDHTEHRVIFDNVENVWEALTKISTYLQFRLKPAI
                     HGKLIGKPFISNAVFIGKGTVIEQGAMIKGPAWIGENCEIR"
BASE COUNT          716 a         1107 c          955 g          623 t
ORIGIN      
        1 cgacatcaag ctggagctcg accgccgcgc gcaggcgctc atcgagtcct tcctcctcga
       61 aaaattcccg gcccacgcga tcttcggcga ggagggcatc cgcggcgacc agtcgagcga
      121 gttccaatgg atcatcgacc cgatcgacgg cacggtgaat tacttctacg gcatccccca
      181 tttctgcatc tccatcgccc tgcgccaggc ggggaaaatg atcgtcggcc tgatctacga
      241 cccctcgcgc gacgaattgt ggaccgccac cgccggcggc cccgcccggc tcaacggcca
      301 gccgatccgc gtcagcccgc gcacgcagct ctccgaatgc acggtctcga tcggcttctc
      361 caagtccgct tcggccatcg acaaaggcat gaagctcttc gagcgaatgg tccgctccgc
      421 caagaaatgc cgcatgcagg gcagcgccgc gctcgacctg gtttacgtgg cctccgcccg
      481 gctcgacgcc tacatcgaaa gccagatcag cctctgggac ttcgccgcgg gcatgctgat
      541 cctcgagcgc gccggcggaa aattccagat gaccgaactc gcggacaaac ccggcaaata
      601 cgccgtcgtg gcctccagcg gcaacgtgga cttgggatta tgaattccga aatccaaaat
      661 ccaaaatcca aaatggaatg agccgctccg ggatcggcta cgacgtgcac cgcttcgcgg
      721 cggagcgcaa attgatcctc ggcggcgtgg agattcccta catgatgggg ctcgacgggc
      781 attccgacgc ggacgtgctt tcccacgcga tcgccgattc gctgctcggc gcggcggcgg
      841 aaccggacat cgggcacctg ttttcgaata ccgatcccac catcgccggc atcagcagcc
      901 tgacgattct ctccaaggtc cgcgacgtgc tggccgcgaa gggttaccgc atcgtcaacg
      961 tggattcgac cgtggttgcc gaagcgccga agatctcccc gcatctggcc ggaatgaagg
     1021 cgaatatttc caaggccctg ggcattgagc ccgccgacgt gggcgtgaag gccaccacca
     1081 acgaatccat gggtttcatc ggccgcgagg aaggcatcgc cgcgctggcg gtggcttcca
     1141 ttgaaaagaa ttaacggatt ttaaccacaa agaacgcaga gagcacaaag gtatctaaaa
     1201 gaccgcggtt tcttatccgc ttgcctattc atgcgctctt tgcgttcttt gcggctaaat
     1261 tgtttcccgt caatcccgtt tagtttgtgg ccctgaactt cttcaatacg ctcacccgcg
     1321 aaatcgaaac tttccagccg ctcgacccgg cgggccggcg ggtgaagctc tatacctgcg
     1381 gcccgacggt ctataactac gcccacatcg ggaatttccg cgcgtatctc ttcgaggacc
     1441 tgcttcagcg gcacctggaa taccgcggct ttgtcgtgga tcgtgtcatg aacctcacgg
     1501 acgtggatga taaaacgatc cgcggctgcc ggacggcggg cgtgccgctg ggcgtcttca
     1561 cgaagacctt caaggacgcg ttttttgcgg atctcgacac gctgcgcatc aagcgcgcca
     1621 atcattttcc cgaggccacc gcggcgaacc agatcgagcg catgatttcg atgatcgcca
     1681 cgctcgtcga gcgcggccac gcgtatcagg ccgaggaccg atcggtttat ttccgcatcc
     1741 gcagcttcca gaactatggc cgactcgcgc acatcaatct cgacgagctc cagccctcgg
     1801 gccgcgtgag cagcgacgaa tacgagaagg aaaacatcgg cgacttcgcg ctctggaaag
     1861 cctgggacga agccgacggc gacgtggcct gggacagccc atggggccgc ggccggcccg
     1921 gctggcacat cgaatgcagc gccatggcca ccggcttgct cggcccgaaa ctggatattc
     1981 actgcggcgg cgaggataac attttccccc accacgaagc cgaaatcgcc cagagcgaat
     2041 cggtcaccgg cgagaaattc gtgcaatact ggctgcactg caaacatctc atggtcgagg
     2101 gccagaagat gtcgaaatcc gcgggcaatt tttacacgct gcgcgatctg ctcgaaaagg
     2161 gctggagcgg ccgcgaggtg cgttacctgc tgctctccgt gcgctaccgc gagccgctga
     2221 acttcacgtt cgagggcctc gccggcgccc gcgccgcgct gcggcgcctg gacgaatggg
     2281 gcgcccgcct cgagcaggcc gcccagggcg cccccgccgc cgagacgccg gcgggactga
     2341 acctcgacgc cttcggcgcc gcgctggacg acgacctcaa catctccgcc gcgctgggct
     2401 cgctcttcga catcgtccgc gaatccaacc gcctgatttc cgaaaaccag ctcccgcctg
     2461 cccaggccag gggattgctg gagtggcgcg accgggtttt cagcgtcctc gccttcgaac
     2521 cggaagcgga acccattccc gccgaagtcg ccgcgctgct cgaacaacgc gccgccgccc
     2581 gcgccggcaa ggattgggcc gccagcgaca cgctccgcga ccaaatcgcc gctctcggct
     2641 ggatcgtcaa agataccaag agcgggcagc aggtaacgaa gaagagttga ccgcggatgg
     2701 cgcggataac cgcggataaa catgcttcat cccgaacctt ccgaacagat cattggagcg
     2761 gcaatgaatg tgctgaatgc cctgaagccg gggctggatg aaaaacttta cgaaaacgcg
     2821 ccggtcgtgg agttgcaatc acgcgggttg ggaatttctc aaccgtcccg atttcccgta
     2881 acctataaag ggatgtccca tcggaaccct gatcccggat ctgattgtgg aaaacaaaat
     2941 catcgtggat gccaaaacgg tcgctgcctt cactgaaaat cattccgcgc agatgctcgg
     3001 ttatctcgca atgaccggat tggaacttgg tattcttctc aatttcaaat acgccaaatt
     3061 ggaatggacg cgcctcgcca aatcccgcct cctctccgaa tgatttttat ccgcgattat
     3121 ccgcgtaatc cgcggtaaaa aaatcccatg ttcacgcccg aaacctatct ctccctcgac
     3181 cacaccgagc atcgggtgat ttttgacaat gtcgaaaatg tctgggaagc gctcacgaag
     3241 atttcgacct acctccagtt ccgcctcaag cccgccatcc acggcaagct gatcggcaaa
     3301 cccttcatca gcaatgccgt cttcatcggc aagggcaccg tcatcgaaca gggcgccatg
     3361 atcaaaggcc cggcgtggat cggcgaaaac tgcgagattc g
//
LOCUS       DATDAY010000423         4400 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_1742728, whole genome shotgun sequence.
ACCESSION   DATDAY010000423 DATDAY010000000
VERSION     DATDAY010000423.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 4400)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4400)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4400
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1742728"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..945
                     /locus_tag="VIS74_08390"
     CDS             <1..945
                     /locus_tag="VIS74_08390"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEY5793303.1"
                     /translation="INTAIHSACSQSLDHTHLITSAVEHSATLKLADHLAKRGFEITR
                     LPVDSLGQLDLAKLEKALHSDTALVSLLWANNETGVLFPIQQIAEICRAKRVPLHSDA
                     VQAVGKIPVNVAELGVSMLSLSGHKLHAPKGVGALFVSKRLRYNPLLRGSQESARRGG
                     TENVASIVALGKAAEIALDHIAEEQTLVRRFRDEFEQAVLARIPGASVNGDPANRLPN
                     TSNLSFEGIESEGALILLDERGICCSAGSACTAGSVHPSHVLKGMGMSNERARASLRF
                     SFGRLNRAGEVPQAIHAVVEVVAKLRSLNAGGSVAMAV"
     gene            1008..2567
                     /locus_tag="VIS74_08395"
     CDS             1008..2567
                     /locus_tag="VIS74_08395"
                     /inference="COORDINATES: protein motif:HMM:NF013347.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEY5793304.1"
                     /translation="MEPDPKNSDKALYQELRTLSWSRLWNEEADRFNEASPEERKKTL
                     AIIRAVGVVFSESGPISQQKEVRQWLRGLLRDPQEKIRRYAMNALPKIGAGAKEEADL
                     LALFEKTEGEREKKFLGKALDKIGGAATLASLNARGDFPVLTEQKVKASIARAENPGA
                     VRLDRALADFADLRIHLHTRQGLEEILRDEAAEAIRKHGKFKITAATSGLVTMTPSAP
                     FSLADIYALRCFGTLGLVVGAGIPANPKDLAALITSPLSRRVLQAFTEGPIRYRLEFI
                     GKGHQRGAIREVVSAVYALCPDILNDARSAPWAIEIHPGESGDTVELRPRLSPDPRFA
                     YRQQDVPAASHPPLAAAMARLAGDGKNEIVWDPFCGSGLELIERAVRGGVKKLYGTDL
                     SDAAISITQMNLSAAKMDAIPAKLVRADFRNFSSIAGLEANSVTLIVTNPPMGKRVPI
                     ADLDVLMRDLFVIAARVLKPGGRLIFPNPLPVQSLPPSLQLRSRQLVDLGGFDCQLEV
                     YQKHAPIPPKK"
     gene            2574..2867
                     /locus_tag="VIS74_08400"
     CDS             2574..2867
                     /locus_tag="VIS74_08400"
                     /inference="COORDINATES: protein motif:HMM:NF019491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dabb family protein"
                     /protein_id="HEY5793305.1"
                     /translation="MIRHTVAFRLNHPAGSPEERDFLQAGRRLAEIPGVEKFEALRQV
                     SSKNPHTFGFSMEFADQAACDHYNAHPLHVAFVRERWFPEVAEFLETDYEPLP"
     gene            complement(2915..>4400)
                     /gene="dnaE"
                     /locus_tag="VIS74_08405"
     CDS             complement(2915..>4400)
                     /gene="dnaE"
                     /locus_tag="VIS74_08405"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEY5793306.1"
                     /translation="YIARKKGLKQIEYAHPLLEQVCSDTYGILVYQEQVQRAANVLAG
                     YSLGQADLLRRAMGKKDKEKMAKERVTFVKGCAEVNRIPAGQANAIFDFLEKFAEYGF
                     NKSHSAAYGLVSYQTAYLKAHYPVEFMAALLTHDASTTDRLAVVIAECKRMDLAVLPP
                     DINRSSLRFAPAEGRSVRFGLASIKNVGEAAMDSAIADRAKLGPFKSLEDFCSRLDSR
                     AVNRKILESLVKCGAFDSFNDNRAALFADIEPALAAAAQVQRDRASGQVSLFGLMEEP
                     APAASKPGRAPVEPWPMAESLGYEKELLGYYVTGHPLDAYAGSLEAEKYTRIADLAEI
                     EKPETREVAGMLILVERKFTKKDNRQFAVVMLEDFTGTLEIMVWDEAFTKYQELIKVG
                     GVVAATVRLTRREEELRASASSFRPLKPRLSKKPVHVKLERQRVSETQLRQILDAAGR
                     FPGKRPLILEFTSSTGGSCLIRSGEEIGIGDERGFLTEIEPAIR"
BASE COUNT          836 a         1444 c         1269 g          851 t
ORIGIN      
        1 atcaataccg cgatccattc cgcctgcagc cagtcgctcg accacacgca cctcatcacc
       61 tcggccgtcg agcacagcgc gacattaaag ctcgcggatc acctcgccaa acgcggtttt
      121 gaaatcacgc gcctgccggt ggattccctc ggccagctcg atctcgccaa actggaaaaa
      181 gccctgcatt ccgacaccgc gctggtctcc ctgctctggg ccaacaacga gaccggcgtg
      241 ctttttccca ttcaacaaat cgccgaaatc tgccgggcca agcgcgtgcc gctgcacagc
      301 gacgcggtgc aggccgtcgg caaaattccc gtcaacgtgg cggaactggg cgtctcgatg
      361 ctctccctct ccggccacaa gctccatgcc ccgaagggcg tcggcgcgct ttttgtcagc
      421 aagcggctgc gttacaaccc gctgctgcgc ggcagccagg aatccgcccg ccgcggcggc
      481 acggaaaacg tcgcctccat tgtcgcgctc ggcaaagccg cggaaatcgc gctggaccac
      541 atcgccgaag aacagaccct cgtccgccgc ttccgcgacg aatttgagca ggcggtgctc
      601 gcgcgcattc ccggcgcctc cgtcaatggc gaccccgcca accggctgcc gaatacgagc
      661 aacctgtcct ttgaaggcat cgaatccgaa ggcgcgctca tcctgctcga cgagcgcggg
      721 atctgctgtt ccgccggctc ggcctgcacc gccggttcgg tccatccgtc gcacgtcttg
      781 aagggcatgg gaatgagcaa tgaacgcgcg cgggccagcc tgcggttttc cttcggccgg
      841 ctcaatcgcg ccggggaagt tcctcaggcc atccatgccg tggtcgaagt cgtcgccaaa
      901 ctccggagcc tcaacgccgg cggatccgtc gccatggcgg tttaagcaat gcttcgcttt
      961 gcctgatttt tcttggaaca gtgaatcgca aagctgctga atcccgcatg gaacccgacc
     1021 caaaaaattc cgacaaagcc ctctaccagg agctccgaac cctttcatgg agccgccttt
     1081 ggaatgaaga ggccgatcgt ttcaatgaag ccagcccgga ggaacgaaag aaaaccctcg
     1141 cgatcatccg cgcggtgggc gtggttttct cggaatccgg cccgatttcc caacaaaagg
     1201 aagtccggca atggctccgt ggcctgcttc gggaccccca ggaaaaaatc cgtcgctacg
     1261 ccatgaatgc gctgcccaaa atcggcgcgg gagccaaaga agaagccgat ttgctcgcct
     1321 tgtttgaaaa aactgaaggt gaacgggaaa agaaatttct cggcaaggcg ctggataaga
     1381 ttggcggcgc cgccacgctg gcaagtttga atgcccgggg tgactttccg gttctaaccg
     1441 agcaaaaggt gaaggccagc attgcccgcg ccgagaatcc gggcgccgtt cggctggatc
     1501 gcgcgctggc cgattttgcc gatctccgaa ttcacctcca cactcgccaa ggactcgaag
     1561 aaatccttcg cgacgaggcg gcggaggcga ttcggaaaca cggaaaattt aaaatcaccg
     1621 cggccacgtc cggcttggtc acgatgacgc cgagcgcgcc tttttccctc gcggatattt
     1681 acgcgctgcg ctgctttggc acgctgggat tggtggtcgg agcgggaatc cccgcaaatc
     1741 caaaggatct agcggctctc attacctcac cgctttcccg gcgcgtgctg caggcgttta
     1801 ccgaaggtcc gatccgatac cgtttggaat ttatcggcaa gggacaccaa cgcggcgcga
     1861 ttcgcgaagt ggtgagtgcg gtttatgccc tttgtccgga catcctcaat gacgcgcgga
     1921 gcgcgccgtg ggccatcgag attcatcccg gcgagtccgg agatacggtg gaactgcgtc
     1981 cgcgcctttc acccgatccc cgctttgctt atcgccagca ggatgtgccc gccgcgtcgc
     2041 atccgccgct cgccgccgcc atggcccggc ttgccggcga cgggaaaaat gaaatcgtct
     2101 gggatccctt ctgcggctcc ggcttggagc tgatcgagcg cgccgtgcgc ggaggggtga
     2161 agaagttata cgggaccgac ctcagcgacg cggcgatctc gattacccaa atgaatcttt
     2221 cggcggcaaa aatggacgcc attcccgcga aattagtccg ggcggatttt cgaaattttt
     2281 cgagcatcgc ggggctggaa gcgaatagcg ttacgctcat cgtcaccaat ccgccgatgg
     2341 gaaaacgcgt tcccattgcg gatctcgatg tgctgatgcg agaccttttt gtcattgccg
     2401 cccgcgtcct caagcccggc ggccggctca tctttcccaa tcccctgccc gtccaatcgc
     2461 tcccgccctc gcttcaactg cggtcccgcc aattggtgga tctgggcggc ttcgattgcc
     2521 agctggaggt ttatcaaaag catgccccca ttcctccaaa gaaataaacg cccatgatcc
     2581 gccacaccgt tgcctttcgc ctcaaccatc cggccggctc gccggaagaa cgggattttc
     2641 tccaagccgg acgccggctg gccgaaatcc cgggcgtcga aaaatttgag gccctgcgcc
     2701 aggtcagctc caaaaatccc cacaccttcg ggttttccat ggagtttgcc gatcaagcgg
     2761 cctgcgacca ttacaacgcg catccgctcc atgtggcgtt cgtgcgggag cgctggttcc
     2821 ccgaggtcgc cgaatttctg gagaccgatt acgagccgct tccctgaaag ggccgccggc
     2881 ctttccgttt tcggaacgca cctccggaaa gccgtcaacg gatcgccggc tcgatctcgg
     2941 tgaggaatcc gcgctcgtcg ccgatcccga tttcctcgcc gctgcgaatg aggcaggagc
     3001 cgcccgtgga gctggtgaat tcaagaatga gcggacgctt gcccggaaac cggcccgccg
     3061 cgtcgaggat ttgccgaagc tgcgtttcgc tcacgcgctg gcgctcgagt ttgacgtgca
     3121 ccggcttttt ggaaagccgc ggcttgagcg gccggaagct ggaggcgctg gcgcgcaatt
     3181 cctcctcgcg gcgggtaagg cgcaccgtgg ccgccaccac gccccccacc ttgatgagct
     3241 cctggtattt tgtgaaggcc tcgtcccaga ccatgatctc cagcgtgccg gtgaagtcct
     3301 ccagcatcac gaccgcaaac tgccggttgt ccttcttggt gaatttccgc tccaccaaaa
     3361 tgagcatgcc ggccacctcg cgcgtttccg gtttttcgat ttccgccagg tcggcgatcc
     3421 gcgtgtattt ctccgcctcc agcgagccgg cataggcgtc gagcggatgg ccggtcacgt
     3481 aataaccgag cagctccttt tcgtagccga gcgattcggc catcggccag ggttccaccg
     3541 gcgcccggcc gggtttggac gcggccggcg ccggttcctc catgaggccg aagagcgaga
     3601 cctgcccgct ggcccggtcc ctttgcacct gcgcggcggc ggcgagcgcc ggctcgatgt
     3661 ccgcaaagag cgcggcgcga ttgtcattga aggaatcaaa ggcgccgcac ttcaccaagc
     3721 tttccaaaat cttccggttc accgcccgcg aatcgagccg cgaacaaaaa tcctcgagcg
     3781 atttgaacgg ccccaatttg gcgcggtccg cgatggccga atccatcgcc gcttcgccca
     3841 cgtttttgat cgaggccagc ccgaagcgca ccgagcgccc ttccgccggg gcaaaacgca
     3901 ggctgctgcg gttgatgtcc ggcggcagca cggcaaggtc cattcgcttg cattcggcga
     3961 tgacgaccgc caggcggtcc gtcgtcgagg cgtcatgcgt gagcagggcg gccatgaatt
     4021 cgaccggata atgcgccttg agataggcgg tctgataaga caccaaccca taagccgcgc
     4081 tgtggctctt gttgaacccg tattccgcaa acttttccag gaagtcgaaa atggcgttcg
     4141 cctgcccggc gggaatccgg ttcacctcgg cgcagccctt cacgaaggtc acgcgctcct
     4201 tcgccatctt ttccttgtcc tttttcccca tcgcgcggcg cagcaggtcg gcctggccga
     4261 gggaataacc cgccagcacg ttggcggcgc gctgcacctg ttcctgataa accaaaattc
     4321 cgtaagtatc cgaacagacc tgttcgagca gcggatgcgc gtattcgatc tgtttcagcc
     4381 ccttcttgcg cgcgatgtag
//
LOCUS       DATDAY010000424         5116 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_1199042, whole genome shotgun sequence.
ACCESSION   DATDAY010000424 DATDAY010000000
VERSION     DATDAY010000424.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 5116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5116)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5116
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1199042"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..412
                     /locus_tag="VIS74_08410"
     CDS             <1..412
                     /locus_tag="VIS74_08410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5793307.1"
                     /translation="HALCGYLPNAAKLDFARLMGARHAQAELARTLLNSFSAICGIIG
                     TQPIPIADLPVLTSLQTLMVGLIIHVSGRKAGPKLIAEFLAAVGVNLGAGLLLREGAR
                     TVVRFVPGFGSAVSGFIAGGGTFLIGKAAIAYFI"
     gene            complement(479..1492)
                     /gene="lgt"
                     /locus_tag="VIS74_08415"
     CDS             complement(479..1492)
                     /gene="lgt"
                     /locus_tag="VIS74_08415"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEY5793308.1"
                     /translation="MSVFVLLAFYVHDLSPFLIQFNDSFGLRWYGLAYVAGFIAGYYL
                     YRWFSRKGYADLPEKEVGDFITWWVVLGTLLGGRLGYVLFYRFGEFLADPWMILRVWE
                     GGMSAHGGILGLLIATMIYARRHRIRWFNLADNLAVTAPIGLFFGRCANFINGELYGR
                     ATTMPWAMQFPKEALDRPELQREILLRVSQEELNRMGAWDLERAVRESEAVRRAAAEV
                     LTPRHPSQLYEALLEGALLFALLFFLRTRCRLPNGILTGTFFIAYALLRSFCELFRMP
                     DAELTGILTRGQLLSIFLILIGAGCLWVAWRQQRYPLRQSLIDSSRAAEKKTSVSGSR
                     TAR"
     gene            complement(1549..2472)
                     /locus_tag="VIS74_08420"
     CDS             complement(1549..2472)
                     /locus_tag="VIS74_08420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY5793309.1"
                     /translation="MVQKLIKAERKARPPENHVGLKPGEGWQERNFLAEVLIPSLFTK
                     RIGQKISKPFPALEGNQIGITWIGHASFLIQTREHSVLIDPNWAKWLKVIKRLRHPGI
                     ELHDLPAIDLVLVTHAHFDHLDRRTLRAVAANQPIVVPEHVGNLVHDLGFQQVHELPR
                     WDTYELGNLKVTLTPAYHWGARMLHDSHRGFGGFHLEYEGRTLFHCGDSAYFPGFTEI
                     GERLQTEIALLPIGAYDAPSKRDVHMNPEQALQAFVELRARTFIPMHYGTFRLSYEPL
                     HEPPRRLMDQAREIGLSERIRILIEGETIVF"
     gene            2550..3170
                     /locus_tag="VIS74_08425"
     CDS             2550..3170
                     /locus_tag="VIS74_08425"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HEY5793310.1"
                     /translation="MKSFLHSGLALGINWGVSEVIPKSALRRELRLRLRAMSAGERAA
                     ASRKIETFVASLPAWQAARRIYAFFPLALEPDLAGWDWAGKSLALPRVRGTELHFCEV
                     ADFTRLEFHPWGMGEPSLLRPPAPPPDLVLVPALGFDRTGRRLGRGGGYYDRFLGALS
                     PAVPRIGVGFDCQLCAAVPAEAHDAPLHAVVTESGVCFSPAGQTPP"
     gene            complement(3121..3942)
                     /locus_tag="VIS74_08430"
     CDS             complement(3121..3942)
                     /locus_tag="VIS74_08430"
                     /inference="COORDINATES: protein motif:HMM:NF026288.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY5793311.1"
                     /translation="MQNTADLWEVLCRDFQTQRSQLTQAQHLEAAAQFLASARQVLPA
                     GSLRLSDAYEMAGDLCHAARGDDRAIPYYLEALRLSSGSGVIESSARVAGKLAILREE
                     ADQLSDAVAMFEQAIALFKQAGDHRQLAALLSHCAALERRLGDQAKARRHYEEAIRVA
                     VELHGESHAEVAVFLNNFGVALCEWRLFAEAEICHLRALQICESAYGSTHPDVGQSLG
                     NLAVLYHIQRDLPRATKFYQAAIQVLSRSRDPNDPDLQMLKAGFDQLGKSTRRIQ"
     gene            4078..4287
                     /locus_tag="VIS74_08435"
     CDS             4078..4287
                     /locus_tag="VIS74_08435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5793312.1"
                     /translation="MIDSPQKRHHDSYFLSLLQDQEVDAFINEILTVRDTTREHVLDA
                     LDSCALIMQPSESRAMIKACVVRKV"
     gene            complement(4317..4919)
                     /gene="recO"
                     /locus_tag="VIS74_08440"
     CDS             complement(4317..4919)
                     /gene="recO"
                     /locus_tag="VIS74_08440"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEY5793313.1"
                     /translation="MVREIAKVGWNCQLLPMKNPVPPTDAIAIGKVRLSDTSLIVTWM
                     TRDCGKVRTAARGALQPKSPFAGKIDLFHEAHLAFQLSPRGDLHTLKEVDLIEPFPAG
                     TLPYAATLAAAYFARWTDLVTQPMEPMGELFSLLQRALAYLRAGKLSAAGVVHFEREL
                     ARLLGIHDPQNDRDVLATLTHYAGRRPTGREDLLAKIPPR"
     gene            4920..>5116
                     /locus_tag="VIS74_08445"
     CDS             4920..>5116
                     /locus_tag="VIS74_08445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5793314.1"
                     /translation="MFSSVLLGPPHSLPDVFGEDALAGMRRHSCLSEPFIDPENLAAH
                     ADQLAGAEVIFSTWGMPKMDEA"
BASE COUNT          963 a         1535 c         1678 g          940 t
ORIGIN      
        1 ccatgccttg tgcggttacc tgccaaacgc ggcgaagctg gatttcgccc gtttgatggg
       61 ggctcgccac gcccaggccg agctggcgcg gacgttatta aattcgtttt cagcgatttg
      121 cgggatcatc ggcacgcagc cgattcccat tgccgacctg ccggttctga cctcgctgca
      181 gactttgatg gtgggtttga tcatccacgt gagcggccgc aaggcggggc cgaagctcat
      241 cgcggaattt ctggcggcgg tcggggtcaa tctgggggcc ggtttgctgc tgcgcgaagg
      301 cgcccggacg gtggtgcggt tcgtgcccgg tttcggcagc gcggtttcgg gtttcattgc
      361 cggcggagga accttcctaa tcggaaaggc ggcgatcgcc tatttcattt aatgcaccaa
      421 gctcagccag ctgcgcggca aggggaagcc ggatagaaag ctgcttgaat cgaacgattt
      481 atcgagcggt tctcgatcct gaaacggacg ttttcttttc ggcggcgcgg ctggaatcga
      541 tcagcgattg ccggagggga taccgctgct gccgccaggc cacccagagg cagcccgcgc
      601 cgatcaaaat gaggaaaatt gacaaaagct ggccgcgggt aaggatgccg gtgagctcgg
      661 cgtcgggcat tcggaacagt tcacaaaagc tgcgcaggag cgcgtaggcg atgaaaaagg
      721 tgccggtgag gatgccattc gggagccggc agcgggttcg cagaaaaaag agaagggcaa
      781 agagcagcgc gccttcgagc agcgcttcat acaattggga cggatgccgc ggggtcagca
      841 cctcggcggc ggcccggcgg acggcttcgg attcccgcac ggcgcgctcg agatcccagg
      901 cgcccatgcg gttgagttcc tcctggctga cgcgcaggag gatttcgcgc tggagctccg
      961 ggcgatcaag tgcttccttg gggaactgca tggcccacgg catcgtggtg gcgcggccgt
     1021 agagttcgcc gttgatgaaa ttcgcgcagc ggccaaagaa gaggccgatg ggcgcggtga
     1081 cggcgagatt gtcggcgagg ttaaaccagc ggatgcgatg tcggcgcgcg tagatcatcg
     1141 tggcgatcag caggccgagg atgccgccgt gcgcggacat gccgccttcc cagacgcgga
     1201 gaatcatcca cggatccgcg aggaattccc caaagcggta aaaaagcacg taaccgaggc
     1261 ggccgccgag cagggtgccc agcaccaccc accacgtgat gaaatcgccg acctccttct
     1321 cggggagatc ggcgtaaccc ttccgggaaa accagcgata caggtaatac ccggcaatga
     1381 atcccgcgac gtaagccaac ccataccagc gcagtccgaa actgtcgttg aattggatca
     1441 ggaaagggct gagatcgtga acgtaaaacg ccagcaggac gaagacggac atggggtgca
     1501 atgattagcc gtggatgaaa cgaatggcca cggatatttc accgcctctc agaaaacgat
     1561 ggtctctcct tcgatcagaa tgcggatgcg ttcggagagg ccgatttccc gggcctggtc
     1621 catcagccgc cggggaggtt cgtgcagcgg ctcatagctg aggcggaagg tgccatagtg
     1681 catggggatg aaggtgcgcg cccgcagttc gacgaaggcc tgcaatgcct gctcgggatt
     1741 catgtggaca tcccgcttgc tcggggcgtc gtaggcgccg atgggcagaa gcgcgatctc
     1801 ggtttggagg cgctcgccaa tttcggtgaa gccggggaaa tacgcgctgt ccccgcagtg
     1861 gaacagcgtg cgcccctcat attccaaatg gaagccgcca aaaccccggt gggaatcgtg
     1921 cagcatccgg gcgccccaat ggtaggccgg cgtcagcgtc accttcaggt tgccgagctc
     1981 ataagtgtcc cagcgcggca gttcgtggac ctgctggaag ccgagatcat gcacgagatt
     2041 gcccacgtgc tcgggaacga cgatgggttg attggccgcc accgcgcgga gcgtgcgccg
     2101 gtcgaggtgg tcaaaatggg cgtgggtgac cagaacgaga tcaatcgccg gcaggtcatg
     2161 aagttcgatt ccgggatgcc gcagccgctt gatcactttc agccacttgg cccagttcgg
     2221 gtcaatgagc acgctgtgct cgcgcgtctg gatcagaaac gacgcgtggc cgatccaggt
     2281 gatgccgatc tggttgccct ccagcgcggg aaaaggcttg gagatttttt ggccgattcg
     2341 cttggtgaaa agcgagggga tgagcacctc cgccagaaaa ttacgctcct gccagccctc
     2401 cccgggcttc agcccgacgt ggttttcagg tggacgcgct ttgcgttcgg ctttgatcag
     2461 cttctgcacc attggcaacg tggattttgg cgcatcccgg cgaaattgca acgcggaagg
     2521 cagggctttc gcaaggccgg aaaacgccgg tgaaatcctt cctgcattcc gggcttgccc
     2581 tgggtataaa ttggggcgtg tccgaagtca ttcccaagtc cgccctgcgc cgcgagctgc
     2641 ggttgcgcct gcgggccatg tccgccggcg aacgcgcggc cgcctcccgg aaaatcgaaa
     2701 ccttcgtcgc cagcctgcca gcctggcagg ccgcgcggcg aatttacgcc ttcttcccgc
     2761 tcgcgctcga gcccgatttg gcgggttggg actgggcggg aaaatcgttg gcgctcccgc
     2821 gcgtccgcgg gaccgaactg catttttgcg aggtcgcgga tttcacgcgg ctggaattcc
     2881 atccctgggg catgggcgaa ccttccctcc tccgcccgcc cgcgccgccg ccggatctgg
     2941 tgctggtgcc ggcgctgggg tttgaccgaa ccggccggcg gctggggcgc ggcggagggt
     3001 attacgatcg tttcctcggc gccctttccc cggcggttcc ccgcatcgga gtgggttttg
     3061 actgccagct ctgcgcggcg gttcccgccg aagcgcacga cgcgcccctg cacgccgtcg
     3121 ttactgaatc cggcgtgtgc ttttccccag ctggtcaaac cccgccttga gcatctggag
     3181 gtccgggtca ttgggatcgc gggaccgcga aagcacctgg atggcggcct ggtagaactt
     3241 cgtggcgcgc ggcagatcgc gctggatgtg gtaaagcacg gccagattgc ccagggactg
     3301 gccgacatcg ggatgcgtgg agccataggc tgactcgcag atctgcaagg cccgcaaatg
     3361 gcagatttcc gcctccgcaa aaagccgcca ttcgcacagc gccacgccga aattattgag
     3421 gaaaacggcc acttccgcat gcgattcccc gtgaagctcg acggcgacgc ggatggcttc
     3481 ctcgtaatgc cggcgcgcct ttgcctgatc ccccagccgc cgctccagcg ccgcgcagtg
     3541 gctgagcaac gcggccagct ggcggtgatc cccggcctgc ttgaacaagg cgattgcctg
     3601 ctcaaacatg gccaccgcgt cgctgagctg gtccgcctcc tcgcggagaa tggccagctt
     3661 cccggcgacc cgggcagagg attcgatgac gccgctgccg gaggaaagcc ggagcgcttc
     3721 caggtaatag ggaatggccc ggtcatcccc gcgcgccgcg tggcaaagat cgccggccat
     3781 ttcgtaggcg tcgctcaggc gcaggctgcc cgccgggagc acctgccggg cggaagccag
     3841 gaattgtgcc gccgcctcca gatgttgggc ctgggtgagt tgggagcgtt gggtttgaaa
     3901 atcccggcaa aggacctccc aaagatcagc ggtgttctgc acaaaatcag cctaggaccg
     3961 caccgctgaa aaatccagat gaactcttgg gtttcgttaa atattcagac cctaaatacc
     4021 cgccggagcc cgataaattc tggacaaatc cgggaatgcg acggataatg gcagcttatg
     4081 atcgactctc cccagaagcg acatcatgac agctactttt tgagcctgct ccaggatcag
     4141 gaagtggatg ccttcatcaa cgaaatcctc acggtgcgtg ataccacgcg cgagcatgtc
     4201 ctggatgccc tcgacagctg cgccctcatc atgcagccct ccgaatcccg ggcgatgatt
     4261 aaagcctgcg tggtgcggaa ggtctgacgc gcgggtttcc ccggatgggg ggaacgtcag
     4321 cgcggaggga ttttggcaag aaggtcttcc cggcccgtcg gccgccgccc cgcgtaatgc
     4381 gtcagcgtcg cgaggacatc ccggtcgttt tgcggatcgt gaatgccgag caggcgggcg
     4441 agttcgcgct cgaaatggac gacgccggcc gcgcttaatt tcccggcgcg caaataggcc
     4501 agcgcgcgtt gcagcaggga aaagagttcg cccattggct ccattggctg ggtgaccaga
     4561 tccgtccagc gcgcgaaata cgccgcggcc agcgtggcgg cgtagggcag ggttccggcg
     4621 ggaaagggct caatcaaatc cacttccttc agcgtgtgca ggtctccccg cgggctgagc
     4681 tggaaggcga gatgcgcctc gtgaaacaga tcgatcttgc ccgcaaacgg gcttttcggt
     4741 tgcagggcgc cccgcgcggc cgtccgcacc ttgccgcagt cccgggtcat ccacgtcacg
     4801 atcaggctcg tgtcggaaag gcgcaccttg ccgatcgcga tggcgtccgt gggcggcacc
     4861 ggattcttca tgggaagaag ctggcaattc catcccacct tggcaatctc tcggaccaca
     4921 tgttttccag cgttctgctc ggcccgccgc attccttgcc cgatgtgttc ggcgaggacg
     4981 cgctcgcggg catgcgccgg cacagctgcc tgagcgagcc gtttatcgac ccggaaaacc
     5041 tggcggcgca cgcggatcaa ttggccgggg ccgaggtgat tttttccact tggggcatgc
     5101 cgaaaatgga cgaggc
//
LOCUS       DATDAY010000425         2059 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_2541842, whole genome shotgun sequence.
ACCESSION   DATDAY010000425 DATDAY010000000
VERSION     DATDAY010000425.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2059)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2059)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2059
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2541842"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..213)
                     /locus_tag="VIS74_08450"
     CDS             complement(<1..213)
                     /locus_tag="VIS74_08450"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY5793315.1"
                     /translation="MSLTDPFVRRPVMTTLVMLAILGSGLLAFQKLPVSDLPNVDFPT
                     ITVSAWLPGASPETMASSVAMPLEKQF"
     gene            complement(210..1334)
                     /locus_tag="VIS74_08455"
     CDS             complement(210..1334)
                     /locus_tag="VIS74_08455"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEY5793316.1"
                     /translation="MTPLSRTARLAGLWLAALLLAGCPKRDAHELAPPPVPVETARVE
                     ARTIPVQRDLIGAVEPMASVQLKSKVQGEIIGVHFADGANVTEGEKLFSIDPRSYAAT
                     LTRAQADVAAAQAGYDNAKEQADRYTTLIKRGVASKEQFTQYRTAAVSQAAALAARQA
                     DLEEARLSKDWTSVTAPISGRAGAALIKRGNIVQANSEVLVVIHQTKPIYVTFSLPEA
                     DLDDVRERMAEGDLAVLARNPENGAVLGEGTLSFVDNAVDRTSGMFVLKGSFPNDNES
                     LWPGQFVDVTLRLAEERGVLTVPTASILDSQSGSQVFVIRDGLAELRKVETGRTAGAD
                     TVIRSGLAAGEVVVSSGQLRLSAGGKVQIKNAPPAAPAEP"
     gene            1848..>2059
                     /locus_tag="VIS74_08460"
     CDS             1848..>2059
                     /locus_tag="VIS74_08460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, biotin carboxyl carrier
                     protein"
                     /protein_id="HEY5793317.1"
                     /translation="MKKNDLAVFKMEREGFKITLKTNAAGMPVVQTYQPVPAALAAPD
                     AGAPAAAAEAKPSNLKEIKSPMVGTF"
BASE COUNT          362 a          692 c          645 g          360 t
ORIGIN      
        1 aaactgcttt tccaacggca tcgccacgga ggaagccatc gtttccggac tcgccccagg
       61 cagccaggcc gaaaccgtga tcgtgggaaa atccacgttc ggcaggtcgc tgaccggcag
      121 tttttggaag gccagcaggc cggagcccag gatggccagc atgacgagcg tcgtcatgac
      181 cggccgccgg acgaagggat cggtcaagct catggttccg cgggtgccgc cgggggcgcg
      241 tttttgattt gcaccttgcc gcccgcgctc aaccggagct ggccgctgct cacgacgact
      301 tccccggccg ccagcccgga acgaatcacg gtatccgccc cggccgtccg gccggtctcc
      361 accttgcgca gctccgcgag gccgtcccgg atcacaaaca cctgcgagcc gctctggctg
      421 tcgagaatgg acgccgtggg aaccgtcagc accccgcgct cctccgccag ccggagcgtc
      481 acgtccacga actggcccgg ccagagggat tcattgtcgt tcgggaaact ccccttgagc
      541 acgaacatgc cggaggtccg gtccacggcg ttgtccacga aggaaagcgt gccctcgccc
      601 aggaccgcgc cattttccgg attccgggcg aggacggcca gatcgccctc ggccatgcgt
      661 tcccggacat cgtcgagatc ggcctcgggc agggaaaagg tcacgtaaat cggcttcgtt
      721 tgatggatca ccacgagcac ctcgctgttg gcctgaacga tgttgccgcg cttgatgagc
      781 gccgccccgg cccgtccgga tatgggcgcc gtcaccgacg tccagtcctt cgagagccgc
      841 gcctcctcca ggtccgcctg gcgggccgcc agcgcggccg cctggctgac ggcggccgtg
      901 cggtattggg tgaactgctc cttcgaggcg acgccgcgct tgatgagcgt cgtatagcgg
      961 tcggcctgct ccttcgcatt gtcatagccg gcctgcgccg cggccacatc cgcctgggcg
     1021 cgcgtgagcg tggcggcgta gctgcgggga tcgatggaaa acaatttctc gccttcggtg
     1081 acgttggcgc catccgcaaa atgaacgccg atgatctcgc cctggacctt ggacttgagc
     1141 tggacgctgg ccatcggttc gaccgcgcca atgaggtcgc gctggaccgg aatcgtgcgg
     1201 gcctccaccc gggcggtttc caccggcacg ggaggcggcg ccagttcgtg agcgtcgcgc
     1261 ttcgggcagc cggcgagcag caatgcggcc agccacaggc cggccagccg cgcggtcctt
     1321 gaaagggggg tcatttcggt cttcattcct tgattcaact cctcaaaccg ccgttccgcg
     1381 caaaaaacag tccgcggcga aatccgcccg ccgcttccag cttgccgggg gggggatgcg
     1441 ccgctcgcat tcctccattt tccgccaatg cccggcatgc gtcggcgaac atgggagctg
     1501 gatttgccgg ggaaaaatct caaaaaagcg ccggttggca tcgtgaaagg cgaattgcgc
     1561 gcgccagccc ccgccggccg gcgggggatt cggacggctc ccgcgccgcg ttcaatatcc
     1621 gatggcggcc gcgcgcttct aattggcgaa gattccgggg tttgacgggg agaggtgaac
     1681 tcacattcac cgaatgaacc tgaattcatt cccgcgcatt gtcaaacgcc ctccggggcc
     1741 cgacctcgct aatatcttga ccccgattgg ctccgcatgg catttctgac ccctccaacc
     1801 ttaccgaccc tgtggactta aaagagatac ggaatttaat tgatctgatg aagaagaacg
     1861 acctcgcggt cttcaagatg gagcgcgagg ggttcaagat caccctcaag accaatgccg
     1921 cggggatgcc ggtggttcag acctatcaac ccgtcccggc agccctggcg gctcccgacg
     1981 ccggcgctcc cgcggcggcc gccgaggcca agccgtccaa cctcaaggaa atcaagtcgc
     2041 ccatggtcgg cacgtttta
//
LOCUS       DATDAY010000426         2128 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_1743813, whole genome shotgun sequence.
ACCESSION   DATDAY010000426 DATDAY010000000
VERSION     DATDAY010000426.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2128
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1743813"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            137..1216
                     /locus_tag="VIS74_08465"
     CDS             137..1216
                     /locus_tag="VIS74_08465"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HEY5793318.1"
                     /translation="MKRKRIGILTSGGDCPGLNAVIRGAFLAADKLNLDVLGFRDGFE
                     GLLPPGDYFPLDRNTTAGIMHLGGTILGTTNRGHFVAKVGSGDKARIDQEIIDKAKQT
                     FHALGLTAIIAIGGDGSLSTALQLQENGVPCIGVPKTIDNDLEATAMTFGFDSAVACV
                     TDALDRLHTTASSHKRAIVLEVMGRHAGWIALHGGVAGGADIILIPEIPFDFEKVAAE
                     VTRRDKEGCKSTLVVVAEGAAPRDGRQMRHHTASGEFRLGGIGEWVAKEIADRTGKET
                     RACILGHLQRGGAPTTLDRILGTRFGVKAVQMVNEGKFGMMVSYQNYQTLEVPIAHAV
                     HRLRRVDPANQMVQAARAVEITFGD"
     gene            1222..1755
                     /locus_tag="VIS74_08470"
     CDS             1222..1755
                     /locus_tag="VIS74_08470"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: protein motif:HMM:NF002636.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HEY5793319.1"
                     /translation="MDAQSADPIRKAIRDIPDFPKPGILFKDIAPILMNPELFRLAIQ
                     HFVNECSVRRVQKIAGIDARGFLFGATVAHELKIGFAPVRKKGKLPGETLSHAYQLEY
                     GEAELEIQTDAFAPGERVVLVDDLLATGGTARAAMSLIESTGAKVVQVQFVIELAFLK
                     GREVLAPTPVYSIVSVD"
     gene            complement(1996..>2128)
                     /locus_tag="VIS74_08475"
     CDS             complement(1996..>2128)
                     /locus_tag="VIS74_08475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HEY5793320.1"
                     /translation="KESLREKIMAFVAKHNRQPKPFRWRKREVRGTQLRNTISNLCN"
BASE COUNT          416 a          661 c          650 g          401 t
ORIGIN      
        1 ggcgcatcat tgccgtgctg ctggggtcca ccgaccgcgc ctcggaaggc ctctccttca
       61 tccggcaggg ctgggggctt tacgaacaat gggcggcggg cggccgggaa acggatagca
      121 ctctttaaat ttttccatga aacgcaaaag aatcggcatt cttacgagcg gcggcgactg
      181 tcccgggttg aacgcggtca tccggggcgc ctttcttgcg gcggacaagc tcaacctgga
      241 tgtgctgggt ttccgggacg gctttgaggg cctgctgcct ccgggggatt atttcccgct
      301 cgaccgcaac accaccgccg gcatcatgca cctcggcggc acgattctcg gcacgaccaa
      361 ccgcggccat ttcgtggcga aagtcggatc gggcgacaag gcgcggatcg atcaggagat
      421 catcgacaag gccaagcaga ccttccacgc gctgggcctg acggccatta tcgccattgg
      481 cggcgacggc tcgctgagca ccgcgctcca gcttcaggaa aatggcgtgc cgtgcatcgg
      541 cgtgccgaag acgatcgaca acgatctcga agccacggcg atgaccttcg gctttgattc
      601 cgccgtggcc tgcgtgaccg acgccctgga ccggctgcac accacggcca gcagccacaa
      661 gcgcgcgatc gtgctcgaag tcatgggccg ccacgccggc tggatcgcgc tccacggcgg
      721 agtcgcgggc ggcgcggaca tcatcctcat cccggaaatc ccgttcgatt ttgaaaaagt
      781 ggccgcggag gtcacgcgcc gcgacaagga gggctgcaag agcacgctgg tggtcgtggc
      841 cgagggggcc gcgccgcgcg acggccggca gatgcgccac cataccgcca gcggggaatt
      901 ccggttgggc ggcatcgggg aatgggtggc caaggaaatc gccgaccgca ccggcaagga
      961 aacccgcgcc tgcattctcg gccatctgca gcgcggcggc gcgccgacca ccctcgaccg
     1021 catcctcgga acccgtttcg gcgtgaaggc cgttcagatg gtgaatgaag gcaagtttgg
     1081 catgatggtc agctaccaga attaccaaac cctcgaagtg cccatcgcgc acgccgtgca
     1141 tcggctgcgc cgcgtggatc cggccaatca aatggtgcag gccgcccgcg ccgtcgagat
     1201 caccttcgga gactagtccg catggacgcc caatccgccg atccgatccg gaaagccatc
     1261 cgggacatcc ccgattttcc caagccggga attctcttca aggacatcgc gcccattctc
     1321 atgaatccgg agctgttccg gctggcgatc cagcattttg tcaacgaatg ctccgtccgc
     1381 cgcgtccaga agatcgcggg aatcgacgcg cgcggctttt tgttcggcgc caccgtcgcg
     1441 cacgagctga agatcggctt cgcgcccgtg cgcaagaaag gcaagctgcc cggcgagacc
     1501 ctctcgcacg cgtatcaatt ggaatacggc gaagcggagc tggaaatcca gaccgacgcc
     1561 tttgcgcccg gggagcgggt ggtgctggtg gacgatctgc tcgccaccgg cggcacggcc
     1621 cgcgcggcga tgtcgctcat cgaatccacc ggcgcgaagg tcgtgcaggt tcaattcgtg
     1681 atcgagctcg ccttcctcaa ggggcgcgag gtgctggccc ccacgcccgt ttattccatc
     1741 gtctcggtgg attaaactga tctgatttct aaatctgcct gtcaagaaac cccgccgctg
     1801 ctcacgtcaa gctcactgcc cagtgaggga agtcttgacg ctgaaggcta tatatactac
     1861 tcatacgagt agtggtatgg acttcctaac ggaaaagcaa cgcaacatat tgggattatt
     1921 cctcttgcga tgggaatgtg gagaacctct cccctctcac aggaaagttt gcaggcactt
     1981 tggatatgct agtgtctaat tgcataaatt tgatatagta tttcgcaatt gagttccgcg
     2041 gacctcacgc ttgcgccagc gaaagggctt gggttggcga ttgtgtttgg ccacaaaagc
     2101 catgatcttc tcccgcaggc tttctttg
//
LOCUS       DATDAY010000427         3244 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_1399247, whole genome shotgun sequence.
ACCESSION   DATDAY010000427 DATDAY010000000
VERSION     DATDAY010000427.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 3244)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3244)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3244
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1399247"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            186..1010
                     /locus_tag="VIS74_08480"
     CDS             186..1010
                     /locus_tag="VIS74_08480"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: protein motif:HMM:NF012444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HEY5793321.1"
                     /translation="MAQTILDRILADVRTEVDAAKYRRSAAELRSRAADLPPALSLPG
                     AIEREFGLIAEIKEKSPSVGRMREANIARAAGVYQDSGIVRAVSVLTNFTHFGMSIER
                     LAEVRQIVAKPVLRKDFIIEEYQIREARAFGADAILLMASVLDAARLKGFYDLTRELG
                     MQALFEVHSSEEILALPADAWVVGINSRKFKTTEGFAGNGSDSPRDFSVDLDTFELAS
                     QLPARTLRVAESGISAETIGRVTATFHAALVGTSLLRDERGIEAGVRAFEDAIISA"
     gene            complement(997..1614)
                     /locus_tag="VIS74_08485"
     CDS             complement(997..1614)
                     /locus_tag="VIS74_08485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5793322.1"
                     /translation="MRLLIRLILLLVAVLAGYALWPRTPHPTQFEPAVVAQLETLAWK
                     SAQNATSLNGTVALYRIYDFQYGLPPRTAVSLSQNTIRSLNIFRTAPDVMDREKALPL
                     LLENLSALKHATKAGFDADAAARQLFTVWTLALQPTPPNALSKAVASYWAILYGKPAS
                     AFTGAANAYADALLANGFVPGSTADWPRVETSLTKAYGQLAATLK"
     gene            1711..2187
                     /locus_tag="VIS74_08490"
     CDS             1711..2187
                     /locus_tag="VIS74_08490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HEY5793323.1"
                     /translation="MSKKEIVAAENLAVLGAKLRAEGRRVVLTNGCFDLLHVGHLRYL
                     EAARALGDRLVVAVNSDESVRALKGPARPINPEGDRAEILSALKCVDYVTIFRTERVT
                     GVIHLLEPDVYAKGGDYTIETLNAEERAALEKCGAAIEILPLVPGKSTTALIEKIA"
     gene            complement(2168..2968)
                     /locus_tag="VIS74_08495"
     CDS             complement(2168..2968)
                     /locus_tag="VIS74_08495"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygenase MpaB family protein"
                     /protein_id="HEY5793324.1"
                     /translation="MVNSQTDSVFGPDSAIWQISRELVLLLGGPCAAILQVAHPQVAL
                     GVKHHSRFREDAFGRLYRTLEAVYTVAFGTRTEAERLKERIAAIHRRVQGSDPAKYSA
                     FSPDAQMWVLATLIQTALEVHERFLHPLPDSLKAEYFHEMKRFGEYFGLERTFGPQTW
                     PEFQTYYQDMLHGPLLASRPECREVARAVALPRGNWLETAAALPIQFLVTEILPSPVR
                     EKLGFRSTPFNRGLAAMGEKLLPILVPRLPAPLRFPRQYREALRRFSQ"
BASE COUNT          635 a          882 c         1118 g          609 t
ORIGIN      
        1 tgctggaatt gcatccttac gaggtgccag agttcgtggt gctgccggtg gagggcgcct
       61 ctccggctta tgcaaaatgg gtggaggaac agaccggttt gatttgaaca gaagttcaca
      121 aaggccgcaa agaggggaaa aactttagcc tttgtggcct ttgcggcctt ctgtaaaaag
      181 tttcgatggc tcagacgatt ctggatcgaa ttttggcgga cgtgcgcacc gaggtggacg
      241 cggcgaaata tcggcgctcc gccgcggaac tgcgttcccg ggcggcggat ttgccgccgg
      301 cgttgtcgct gccgggagcg attgagcggg agtttgggct gatcgccgag atcaaggaaa
      361 agtcgcccag cgtgggacgg atgcgggagg cgaatatcgc ccgggcggcg ggggtttatc
      421 aggattcggg tatcgtccgc gccgtttccg tgctgacgaa cttcacccat ttcggcatgt
      481 ccatcgaacg cctggcggaa gtccggcaaa tcgtggcgaa gccggttttg cggaaggatt
      541 ttatcatcga ggaataccag attcgcgagg cccgggcgtt tggcgcggat gcgattttat
      601 tgatggccag cgtgctggac gcggcgcggc tgaaggggtt ttacgatctc acccgcgagc
      661 tgggcatgca ggcgctgttt gaagtgcatt catcggagga aatccttgcg ctgcccgccg
      721 atgcctgggt ggtggggatc aatagccgga agttcaaaac gaccgaaggg tttgccggaa
      781 acggatcgga ttccccccgg gatttcagcg tggatctgga cactttcgaa ctcgcctcgc
      841 aattgccggc gaggacgttg cgggtcgcgg aaagcggcat ttcggcggaa accatcggcc
      901 gcgtgacggc gacgtttcac gccgcgctgg tgggaacctc attgctgcgg gacgagcggg
      961 gaatcgaagc cggggtccgg gccttcgagg acgcgattat ttcagcgtag ccgcgagctg
     1021 cccgtaggcc ttggtgaggg aggtttcgac gcggggccag tccgccgtgc tgccgggaac
     1081 gaagccgttg gccagcaggg cgtcggcgta ggcattggcc gcgccggtga acgcgctcgc
     1141 gggtttgccg tagaggatcg cccaataact ggccaccgcc ttggaaaggg cgttcggcgg
     1201 cgttggttga agggcgaggg tccagaccgt aaagagctgc cgggcggccg cgtcggcgtc
     1261 aaatccggcc ttggtggcgt gcttgagggc gctcaaattt tcgagcagca agggcagggc
     1321 cttttcccga tccatcacgt cgggcgcggt gcgaaaaatg ttcagggagc ggatggtgtt
     1381 ctgcgaaagg gaaacggcgg tccgcggcgg caggccgtat tgaaagtcgt agatccggta
     1441 aagcgccacc gtgccattga gggaggtggc gttctgcgcg ctcttccagg ccagggtttc
     1501 aagttgggcc acgaccgccg gctcaaattg ggtgggatgc ggcgtgcggg gccaaagggc
     1561 atagcccgcg agaacggcaa ccagcagcag aatgaggcgg atgagtaggc gcatggggtg
     1621 gttgggaagc gaagcttacg cggttcgcgg cgccaaacaa agcccaaatt tgcgttgacc
     1681 cggatgcggg cgggtggcga tgactggggc atgagcaaaa aggaaattgt cgccgccgag
     1741 aatctggccg tattgggcgc gaagctgcgc gccgaaggcc ggcgcgtggt gctgaccaac
     1801 ggctgcttcg accttctgca cgtggggcat ttgcgttacc tcgaagccgc ccgggcgctg
     1861 ggcgaccgcc tggtggtggc ggtgaattcg gatgaatccg tgcgcgcgct caaggggccc
     1921 gctcggccga tcaatccgga aggagaccgc gcggagattc tctcggcgct caaatgcgtg
     1981 gactacgtga ccattttccg cacggagcgc gtgacgggcg tcatccacct gctcgaaccc
     2041 gacgtttatg ccaagggcgg ggattacaca atcgaaacgc tcaacgcgga agagcgcgcc
     2101 gccctggaaa aatgcggcgc ggccatcgaa atcctgccgc tggttccggg aaaatccacg
     2161 accgccctca ttgagaaaat cgcctgagcg cctcgcggta ttgccggggg aaacgcagcg
     2221 gcgcgggcag ccgcgggacg aggatcggca ataatttttc ccccattgcg gccagcccgc
     2281 gattgaatgg cgtggagcga aaaccgagct tttcgcggac gggcgagggg agaatttcgg
     2341 tgaccagaaa ttgaatggga agagccgcgg cggtttcaag ccaattgccc cgcggcagcg
     2401 cgaccgcgcg ggccacttcg cggcattccg ggcggctggc gagcagcggg ccgtggagca
     2461 tgtcctgata atacgtctga aattccggcc aggtctgcgg cccgaaggtt cgttcgagtc
     2521 cgaaatactc gccgaagcgt ttcatttcgt gaaaatactc cgcctttaat gaatccggca
     2581 gcggatggag aaaacgttca tgcacctcga gcgcggtctg gatgagcgtg gccagcaccc
     2641 acatctgcgc gtcgggggaa aaggcggaat atttcgccgg gtcgctgcct tgaacccgcc
     2701 ggtggatcgc ggcgatccgt tccttgagcc gctccgcctc cgtccgcgtg ccgaaggcca
     2761 cggtgtaaac ggcttcgagc gttcgataaa gccgcccgaa cgcgtcttcg cgaaaccggc
     2821 tgtgatgctt cacgcccagc gccacctgcg gatgggcgac ctgcaggatg gccgcgcagg
     2881 ggccgccgag cagcagcacg agttcccggc tgatctgcca aatggcggaa tccgggccga
     2941 agacggagtc ggtttgagag ttaaccacag aggacacaga gagcacagag gggaaaatta
     3001 aaaagggaaa tgtgaattcc ctcttttgtc tttcctccgt gttctctgtg tcctctatgg
     3061 taaaaaaatc agaatgccac gaccgcctcg atttcgacgt cgaaattcag caactggcag
     3121 ccgacggtcg cgcgggcggg tttgtgctcg ccgaaatagt tcgcgtaaac ctcgttcatc
     3181 gccttgaagg tttcggcgtc gaatgtctgc agatacacgc ggcagctcac gacattttgc
     3241 aatc
//
LOCUS       DATDAY010000428         2849 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_1577770, whole genome shotgun sequence.
ACCESSION   DATDAY010000428 DATDAY010000000
VERSION     DATDAY010000428.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2849)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2849)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2849
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1577770"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..677)
                     /locus_tag="VIS74_08500"
     CDS             complement(<1..677)
                     /locus_tag="VIS74_08500"
                     /inference="COORDINATES: protein motif:HMM:TIGR01856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase"
                     /protein_id="HEY5793325.1"
                     /translation="MSAPVLYESHSHTPLCRHAIGEPEQYAAMAEARGLKGIVITCHC
                     PLPGGFAGDVRMRPEQFETYVEMVARARAAWLGRVDVRLGLESDYYPGVEPWLEKLHA
                     RVPLHHVLGSVHYQMGDYRKRFFTGDVFAYQQLYYDHLARAAETGLFDTLAHPDLIKN
                     EAPAEWIFPRIRPFVERALDRIAATGVAMELNTSGLNKLLPEMNPGIPQLRLMAERSI
                     PVVIGADA"
     gene            948..>2849
                     /locus_tag="VIS74_08505"
     CDS             948..>2849
                     /locus_tag="VIS74_08505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5793326.1"
                     /translation="MSTFPAVRRFLGLLWLAVPFAAAQPLPETQVPAPLRDWVTWATW
                     DAPHRNCPAPYFNSKKHLCFWPGRLDLTAAKTGGAFTLEVTVFSESWVPLPGGKNAWP
                     LEVKLAGQPLPVLERDGRPSVRLAPGKYRLEGIYRWNDLPQSLPLPREIGLLTLTLEG
                     QAVEAPAWDAQGLLWLKRDGSSEETDKDFLATKVYGLLEDGIPLWLQTEIELVVSGKS
                     REEELGAILPAGWKLSAVESPIPVAVDETGRVKAQVRAGKWTIKLRAFRLDDPRQFQF
                     AADAPPGMAEELIAFRSKPDFRMVEIVGAPSIDVSQTTFPAQWREFPVYRWDTAAPFQ
                     MEERMRGMGLQQPEGLTIHRELWLDSAGRELTFRDRLTGSMQQIWRLDAAAGQDLGSV
                     RSNGVGQLITRNPATQAPGVEIRTRNLNLEATGRSQIAGGLSATGWQTDADAVQVTLN
                     LPPGWRLFALFGPDWVRGDWLTAWSLLDLFLLLLFTLAVFRLWGLWPAVLAFLAFGLS
                     YHEPGAPRYAWLALLVPLALLRVVPAGWGHRLITVGKWLVIVVLVFALVPFLARQVRQ
                     AIYPQLEDAITNRQEFTVSRKPGDSNMPVPSQMPLEEAQKEAVSDYSFGLSSQKQSRR
                     QSEATKSNLF"
BASE COUNT          494 a         1002 c          842 g          511 t
ORIGIN      
        1 gcgtccgccc cgatcacgac gggaatgctc cgttcggcca tcaatcgcag ctgcggaatg
       61 ccgggattca tctccggcag cagcttgttc agtccgctgg tgttcagctc catcgccacc
      121 cccgtcgcgg cgatgcgatc cagcgcccgt tcaacgaatg gccggatgcg gggaaaaatc
      181 cattccgccg gcgcctcgtt cttgatgagg tccggatgcg ccagcgtgtc gaacaatccg
      241 gtctccgccg cccgggcgag atggtcgtaa taaagctgtt gatacgcaaa gacgtcgccg
      301 gtgaaaaacc gcttgcggta atcgcccatc tggtaatgca ccgagcccag cacgtgatgc
      361 agcggcacgc gggcgtggag cttttccagc cagggttcga cgccggggta ataatcgctc
      421 tccaacccca gccgcacgtc cacgcgcccc agccaggccg cgcgggcgcg ggcgaccatc
      481 tccacatacg tctcgaattg ctccggccgc atccgcacgt ccccggcaaa tccccccggc
      541 agcggacaat ggcaggtaat gacaatcccc ttcaacccgc gcgcctcggc catggccgcg
      601 tattgctccg gctcgccaat ggcatggcgg cagagcggcg tgtgggagtg gctttcgtaa
      661 aggacgggcg cggacatctg cctccactct cgccgagagc cccgcccaac gcaaatggaa
      721 ccatgccgcc gggttcccca aatcgtctca cgcaaagccg caaagcggcg ggaagtggtg
      781 actggcaact agcggatggt gaatggcgcc gcgaaacgag cttccgcttc aattttaacg
      841 catttcttca actcttcttt gcggctttgc gcctttgcgt gaaacgccgc ccacattcca
      901 ctcgcctcca aaccatttgc ctgaaaggat gatgccaccc tctcgccatg agcactttcc
      961 ccgccgttcg ccggttcctt ggccttcttt ggcttgccgt cccctttgcg gccgcccagc
     1021 cgcttccgga aacgcaagtc cccgccccgc tgcgggattg ggtgacctgg gcaacctggg
     1081 atgccccgca ccggaactgc ccggcgccct atttcaattc gaaaaagcac ctctgcttct
     1141 ggcccggccg cctggatttg acggccgcca aaaccggcgg cgccttcacg ctcgaggtca
     1201 ccgtcttcag cgaatcctgg gtgcccctgc ccggcggcaa aaatgcctgg ccgctggaag
     1261 tcaaactggc cggacagccg ttgcccgtgc tcgaacggga cgggcggccc agcgtccggc
     1321 tggctcccgg caaataccgg ctggagggaa tctaccgttg gaatgacctg ccgcaatccc
     1381 taccgttgcc ccgggaaatc ggcctgctca cgctcaccct ggagggtcag gcggtcgaag
     1441 cccccgcctg ggatgcgcag ggcctgctct ggctgaagcg ggatggctcg tcggaggaaa
     1501 ccgacaagga tttcctcgcc accaaggttt acggcctgct ggaggatggc attccgctct
     1561 ggctgcaaac cgagatcgaa ctcgtcgttt ccggcaaaag ccgcgaggag gagctcggag
     1621 cgatccttcc cgccggctgg aaactgtccg cggtggaaag cccgatcccc gtcgccgtgg
     1681 acgaaacggg ccgcgtcaag gcgcaggtcc gggccggaaa atggacgatc aaactccggg
     1741 cgttccgcct cgacgatccg cggcaatttc aattcgccgc cgacgcgccg ccggggatgg
     1801 cggaagaatt gatcgccttc cgttcgaagc cggatttccg catggtggaa atcgttggcg
     1861 ctccctccat cgacgtctcg caaaccacct tcccggcgca gtggcgggaa ttccccgttt
     1921 accgctggga caccgccgcg ccctttcaaa tggaggagcg catgcgcggc atgggcctgc
     1981 aacaacccga gggcctgacc atccaccggg aactctggct cgattcggcg ggacgcgagc
     2041 tcaccttccg cgaccgcctc accggcagca tgcagcaaat ctggcggctc gacgccgcgg
     2101 cgggccagga tctcggttcc gtccgcagca atggcgtggg ccagctcatc acgcggaacc
     2161 ccgcgacgca ggctcccgga gtggaaatcc gcacccgaaa cttgaacctc gaagccaccg
     2221 gccggtccca aatcgcggga ggcctgtccg ccaccggctg gcaaaccgac gccgacgccg
     2281 tccaggtcac gctgaatctc ccgcccggct ggcggctctt cgcgctcttt ggcccggact
     2341 gggtgcgcgg cgactggctc accgcctgga gcctgctgga tctcttcctg ctcctgctct
     2401 tcaccctcgc cgttttccgg ctctgggggc tctggccggc cgtgctcgcc tttctcgcct
     2461 tcggcttgtc ctatcacgaa cccggcgcgc cgcgctacgc ctggctggca ctcctggttc
     2521 ccctcgccct gctccgcgtg gtcccggcgg gctggggcca tcggctcatc acggtcggaa
     2581 aatggctggt gatcgtcgtg ctggtgttcg cccttgtccc cttcctcgcc cgccaggtgc
     2641 ggcaggcgat ctacccccaa ttggaagacg cgatcacgaa ccggcaggaa ttcaccgttt
     2701 cccgcaaacc cggtgattcc aatatgccag tcccttctca aatgccgctt gaagaggcgc
     2761 agaaagaggc ggtttccgac tattccttcg gtctcagctc ccagaagcaa agccggcgtc
     2821 aaagcgaagc caccaagagc aacctcttt
//
LOCUS       DATDAY010000429         2034 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_2110199, whole genome shotgun sequence.
ACCESSION   DATDAY010000429 DATDAY010000000
VERSION     DATDAY010000429.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2034
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2110199"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(435..1586)
                     /gene="cfa"
                     /locus_tag="VIS74_08510"
     CDS             complement(435..1586)
                     /gene="cfa"
                     /locus_tag="VIS74_08510"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: protein motif:HMM:NF008686.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HEY5793327.1"
                     /translation="MSAETVVKRPRMASRAQFAVRDILASADILIDGPRPWDIQVHDD
                     RFHARVLAEGTLGLGESYMDGWWDCDALDEMCCRAIRARLEERIPLNLRTVVSIAESV
                     LLNLQNKRRALRVGEQHYDLGNDFFEAMLDPSMQYSCAYFLGTSDLAEAQELKLDLIC
                     RKLGLKAGMRLLDIGCGWGGLAKYAARHHGCEVVGITISREQQAYAEAACRGLPIEIR
                     LQDYRDVSESFDRIVSVGMMEHVGRKNYRTYLETAGRCLQEGGRFLCQTIANAQSIPH
                     CDPWITRHIFPNSMLPSASEVAGAAEGVFVLEDVQNFGADYDDTLLAWEKNFRATWDQ
                     FADRYGERFRRMWRFYLLSCAGAFRARSMQLFQFLFSKGGIEGGYTLMR"
BASE COUNT          416 a          619 c          602 g          397 t
ORIGIN      
        1 cctttccgtt cgcaggcctc tcgtaacgtc cgcccgccct ggcagcaaaa atcaatttgg
       61 aagaactgga aaatccggga acgatcaggc cgctcggcga cgagttcgcc aaccgttcgg
      121 ttggccagcg gcgtgcctgc gggggagctg atattggggc gatgactcat ggtggaaacc
      181 ttttacgaga gatcgccgaa taaataaaga taaaactatc ttttattctt ctcgaaaaaa
      241 cttccccgcc gcgcattcga ctcccgccgt caatgacgag tggcaaccgg gggaattcct
      301 cgagccggcg gcttcgattc gattttacgt gcccgaactc cagagctgcc catggcgttt
      361 ttgagctttg gaagcgccat tcaaaacggc gggaacttga ttcggcgacg atccaaggca
      421 cggatgggat cggctcatcg catgagggtg tatccgccct cgattccccc tttggagaaa
      481 agaaactgga agagctgcat gctgcgggcg cggaaggctc ccgcgcagct caacagataa
      541 aatcgccaca tccgccggaa ccgctccccg tagcgatcgg caaattgatc ccacgttgcg
      601 cggaaattct tttcccaggc gagcagcgtg tcgtcgtaat ccgcgccgaa gttttggacg
      661 tcctcgagga caaacacccc ctccgcggcc cccgcgacct cggaggcgga gggaagcatg
      721 gagttcggaa aaatatgccg ggtgatccac gggtcgcaat gcgggatcga ttgcgcattg
      781 gcgatggtct ggcaaaggaa tcggcctccc tcctgaaggc accgcccggc ggtttccaga
      841 tacgtgcggt agttttttcg gccgacgtgc tccatcatcc cgacactgac gatgcgatcg
      901 aaggactccg aaacatcgcg gtagtcctgc aggcgaattt cgatgggcag tccgcgacag
      961 gccgcctccg cataagcctg ctgctcgcgg gaaatggtga tgcccacgac ctcgcagcca
     1021 tggtgccggg ccgcatactt cgccagcccg ccccagccgc atccgatgtc gagcaggcgc
     1081 atgccagcct tcaggccgag tttccggcag atcaaatcca gctttaattc ctgggcttcg
     1141 gcaaggtccg acgtgcccag aaaataggcg caggaatact gcatggaggg atcgagcatc
     1201 gcctcgaaaa agtcgttgcc gagatcgtaa tgctgctcgc cgacccgcag cgcgcggcgt
     1261 ttgttctgga ggttgaggag caccgattcg gcgatggaaa ccaccgttcg taaattgagg
     1321 ggtattcgct cctcgagccg cgcccggatg gctcgacagc acatctcgtc cagggcatcg
     1381 caatcccacc agccgtccat gtaggattct ccaaggccca gcgtgccttc cgcgagcacg
     1441 cgcgcgtgga agcgatcatc gtgaacctgg atgtcccaag gccgcggccc gtcgatgagg
     1501 atgtctgcgg aggccagaat gtctctcacg gcaaactgcg cccgggacgc cattcgcggc
     1561 cgtttgacga cggtctcggc ggacatggca ggcatacgca tggactggcg ttccatcatt
     1621 cacagagttt ggcgtcgcgc aggcacgggg gggagtggcg acgcccaaat gtatcaacga
     1681 cagctaacta tgtgaagacc ttactttccg ccgtttgtgt gctcgaccga acatggctcg
     1741 aaatttcggc ccatgaacac ccggcacgcc cccgcagccg gcgacgacag ctttgaccga
     1801 tacctccgga ctccagccgg cggagtgggt cgccagtaat tcacctcggc gtcgggcgtt
     1861 tgattttcac atgcgccgcg tccgactcgt gcgatacgtt agtaaggctc gtgattagga
     1921 ggacttgtca tgccgcagct attccccccg tccgcaaata cgctggtgat cgtcatcatt
     1981 ggcggcagcc tcgccgtcat cggcggcctt gcctgggcgc tcagcgagtt gaac
//
LOCUS       DATDAY010000430         5179 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_2112614, whole genome shotgun sequence.
ACCESSION   DATDAY010000430 DATDAY010000000
VERSION     DATDAY010000430.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 5179)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5179)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5179
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2112614"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2657
                     /locus_tag="VIS74_08515"
     CDS             <1..2657
                     /locus_tag="VIS74_08515"
                     /inference="COORDINATES: protein motif:HMM:TIGR00915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY5793328.1"
                     /translation="IKGVGDIQFFGAREYSMRIWLNPEKIASLNMTAGDVVQALREQN
                     MQIPAGTIGGQPAPPGLDFQMTVTTQGRLEDVSQFEDIVIKTGAAGEVVRLKDVARVE
                     LGAKDYTIVSRLDGKPAINIGVFQAPGSNALEVSQNVRREMERLKERFPPGVDYRIIY
                     DTTMFVREAITSVVHTIFEAFLLVVIVVLIFLQSWRSTLIPILAVPVSLIGTFAIMLP
                     FGFSLNLLTLLGLVLAIGIVVDDAIVVVEAVEHHLSEGLSRREATLKAMEEVAGPIVA
                     ISLVLSAVFIPTAFIPGLTGSFYRQFALTIAFSTILSMVNSLTLSPALCALFLQAPKE
                     KKDVLGRLLNGALGWFFRLFNRTFGGVQQLYVRTLKGFFRFKYLALAGYLVLLGLTAF
                     LFQKVPTGFIPETDQGVMFVNVELPEGASIERTAKVMDKVEAILKNTPGIANTINRIG
                     SSSVTQATAPNTGTVICVFEPFEERKHDPKESLRGILGHINPQFAKITEARVLAFPAP
                     SVRGLGTTGGAKMMVQDRAGGSPAELEHALGRVIQDGAARPELARLFTLFRANTPQIY
                     ADVNRTQAKSKGVDISSIAEALQFYLGSIYVNDLTLYGKPFQVTAQADGQFRARPDDI
                     LQLRTRNAAGEMVPLGSLVDLKEITAPSRVMRYNLFPAAELNSNAAPGYSTGQVIQAL
                     EETARATLPATYGFEWTETALQEILAGNSALYVFPMCVLFVFLLLAALYENWALPLAI
                     ILIVPLCVLCALTGIWISGGDNNIFTQIGFVVLIGLATKNAILIVEFAKLIQERDGKS
                     VVEAALEASRLRLRPILMTSFAFILGVVPLVLASGAGYELRRSLGTAVFSGMVGVTFF
                     GLVLTPVFYVVIRTLQERRKKRPAVTPA"
     gene            2689..3180
                     /locus_tag="VIS74_08520"
     CDS             2689..3180
                     /locus_tag="VIS74_08520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HEY5793329.1"
                     /translation="MPSFDVVSEVNKMEIKNAVDAAQKELTTRFDFKGANAEIELEAK
                     TDLIKLRVADSAKIKALKEIAMGKLAKRNIDLRNVETKDAAISPLGHATQELQIKQGI
                     DGDKAKEITKAIKAGFPKVQSTLQDRQIRVTGKSRDDLQAVIAFLRGKDFEIGLGYTN
                     FRD"
     gene            complement(3273..4889)
                     /locus_tag="VIS74_08525"
     CDS             complement(3273..4889)
                     /locus_tag="VIS74_08525"
                     /inference="COORDINATES: protein motif:HMM:NF024909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatA and WFA domain-containing protein"
                     /protein_id="HEY5793330.1"
                     /translation="MTFALPAGLFLLAALPVAAGLYLRRVRPPEQRVPSLLLWEQVLR
                     LNARARGWGRIRAWLSLLLQLLILLLLILAVSRPEADRSPAAARVIVLDTRDRMQAVD
                     GLHQSALAKARRFAEAAAEMAREDSPIALVALGTADGSPRLIHPFSSDPRALREALKH
                     LPATPGDGDLPAAIQFAREIAAAAGPAAEVQVLTDRPPAESERGVHFVTFGNPAENWG
                     FAAAGAREANGRALLFLRPVSHANAPREVQLDLEQAGRLLDTRRLRLNPGEPRDLHFD
                     FPVGSEPYLARLRAKDALDADQSVQLTVPNPPPRRVLLVSEGTWFVEKALRADESLQF
                     EMLRPGEWNPALSEAFDVTLFDGQIPGNFLEKGRFGFLGTAPGLTPGTAANPAVVKFD
                     SHHPLLRSLNPGHWRIGEQAGQPAGSGYEEVVTGTQGPLLIARAAEEVRWAALLFPPE
                     KSDLPLRASFPVLLQNLLDWVTRDSRDSAAVIAPLDAEGGDLKTAAASPLPALQRWGF
                     GYFPIWRWLALAAFALLLAEWITYHRRVTE"
     gene            complement(4886..>5179)
                     /locus_tag="VIS74_08530"
     CDS             complement(4886..>5179)
                     /locus_tag="VIS74_08530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5793331.1"
                     /translation="RRLAASRFEVALIHLLDPAEILSPLRGDWILRDSETGRGERIVA
                     TPALLQAYSRRVAAFSAELDAFGRKRRMAMHRARSDERFEDVLLRELRGGLLK"
BASE COUNT          955 a         1716 c         1638 g          870 t
ORIGIN      
        1 gcatcaaggg cgtgggcgac atccagtttt tcggcgcgcg ggaatattcc atgcgcatct
       61 ggctgaatcc ggaaaaaatc gcctcactca acatgaccgc cggggacgtg gtgcaggcgc
      121 tgcgcgagca gaacatgcag atccccgccg gcacgatcgg cggccagccg gcgcctccgg
      181 ggttggattt tcagatgacc gtcaccacgc agggccgcct ggaggacgtg agccagttcg
      241 aggacatcgt catcaagacc ggcgcggccg gcgaggtggt ccgtctcaag gatgtcgccc
      301 gcgtggagct cggcgcgaag gattacacca tcgtttcccg gctcgacgga aagccggcca
      361 tcaatatcgg cgtcttccag gcccccggct cgaatgcgct ggaggtctcg cagaacgtgc
      421 gccgggaaat ggagcggctg aaggagcggt tcccgccggg cgtggattac cggatcattt
      481 acgacacgac catgttcgtg cgcgaagcca tcacgtcggt ggttcacacc attttcgagg
      541 ccttcctgct cgtcgtcatt gtcgtgctga tcttcctgca aagctggcgc tcgacgctga
      601 tcccgatcct cgccgtcccc gtgtcgctca tcggcacgtt cgccatcatg ctgccgttcg
      661 gcttctcgct gaatctgctc accctgctcg ggctcgtgct ggcgatcggc atcgtcgtgg
      721 atgacgccat cgtcgtcgtt gaggccgtgg agcatcattt gtcggaaggc ctgagccggc
      781 gggaggcgac gctgaaagcc atggaggaag tggccgggcc gatcgtggcc atctcgctcg
      841 tgctctccgc ggtcttcatc ccgacggcct tcattcccgg gctgaccgga tcgttttacc
      901 gccagttcgc gctgaccatc gcgttctcga cgattctttc gatggtcaat tccctgacgc
      961 tcagccccgc gctctgcgcg ctgttcctgc aggcgccgaa ggaaaagaag gacgtgctcg
     1021 ggcggctgct caacggcgcg ctcggctggt ttttccggct gtttaaccga accttcggcg
     1081 gcgtccagca gctctatgtg aggacgctca agggattttt ccggttcaaa tacctcgcgc
     1141 tggccgggta tctcgtcctg ctcggcctca cggccttcct gttccaaaag gtgcccaccg
     1201 ggttcattcc cgagaccgac cagggcgtta tgttcgtgaa cgtcgaactg cccgagggcg
     1261 cctccatcga gcgcaccgcc aaggtcatgg acaaggtgga ggcgatcctg aaaaacacgc
     1321 ccggcatcgc gaacaccatc aaccgcatcg gcagctcgag cgtgacgcag gccaccgcgc
     1381 caaacaccgg cacggtcatc tgcgtgttcg agcccttcga ggagcgcaag cacgatccga
     1441 aagaatccct gcgcggcatc ctcggccaca tcaatcccca gttcgccaaa atcaccgagg
     1501 cccgcgtgct ggcgttcccc gccccctccg tccgcggcct gggcacgacc ggcggcgcga
     1561 agatgatggt gcaggaccgc gccggcggca gccccgcgga gctggagcac gcgctcggcc
     1621 gggtgatcca ggacggcgcg gcgcgccccg agctggcgcg cctgttcacg ctcttccggg
     1681 cgaatacgcc gcagatttac gcggacgtga accgcaccca ggccaagagc aagggcgtgg
     1741 acatcagctc catcgccgaa gcgctgcagt tttacctcgg ctcgatctac gtgaacgatc
     1801 tgacgctcta cggcaaaccc ttccaggtca ccgcccaggc cgacgggcaa ttccgcgcgc
     1861 ggccggatga cattctccaa ctgcgcaccc gcaacgccgc cggcgaaatg gtgccgctcg
     1921 gcagcctggt ggacctcaag gaaatcacgg cgccctcgcg cgtcatgcgc tataatttgt
     1981 ttcccgccgc cgagctaaac tcgaacgccg cgcccggcta cagcacgggc caggtgattc
     2041 aggccctgga ggaaaccgcc cgggcgacgc tgcccgcgac ctatggcttt gaatggaccg
     2101 aaaccgcgct gcaggaaatc ctcgcgggca acagcgcgct ctacgtcttc ccgatgtgcg
     2161 tgctgtttgt cttcctgctg ctcgccgcgc tgtatgaaaa ctgggcgctt ccgctggcca
     2221 tcatcctcat cgtgccgctc tgcgtgctct gcgcgctcac cggcatctgg atttccggcg
     2281 gggataacaa catcttcacg caaatcggct tcgtcgtgct gatcggcctg gcgacgaaga
     2341 atgccatcct catcgtcgaa ttcgccaagc tcatccagga gcgcgatggc aaatcggtcg
     2401 tcgaagccgc gctggaggcc agccggctgc gcctgcggcc gattttgatg acctccttcg
     2461 ccttcatcct cggcgtggtg ccgctcgtgc tggccagcgg agcgggttat gagctgcgcc
     2521 gctcgctggg aacggccgtg ttctccggca tggtgggcgt gaccttcttc ggcctggtcc
     2581 tgacgccggt cttctacgtc gtgatccgca cgctgcagga acgccggaag aagcggcccg
     2641 cggtgactcc cgcttgaatc cggcggagcg gggatttagg ctgggcgcat gccctcattc
     2701 gacgtcgttt cggaagtcaa caaaatggag atcaagaacg cggtggacgc cgcgcagaag
     2761 gaactcacca cgcgatttga cttcaagggc gcgaatgccg aaatcgaact cgaggcgaag
     2821 accgatctca tcaagctgcg cgtcgccgat tccgccaaaa tcaaggcgct gaaggaaatc
     2881 gcgatgggca aactcgcgaa gcggaacatc gatctccgca acgtggagac gaaggatgcc
     2941 gccatttccc cgctcggcca cgccacgcag gagctgcaga tcaagcaggg catcgacggc
     3001 gacaaggcga aggaaatcac caaagccatc aaggccggtt ttcccaaggt ccaatccacg
     3061 ctgcaggacc gccagatccg cgtgaccggc aagagccgcg acgacctgca agccgtcatc
     3121 gccttcctgc gcgggaagga ttttgaaatc ggcctgggct acacgaattt ccgcgactga
     3181 tttgaacaga aggtcacaaa gaacgcaaag atcggacaaa aaatccgtcc ctttgtgcgc
     3241 tttgtggcct tctgttcaaa aattctttcc gcctactccg tcacccggcg gtgatacgtt
     3301 atccactccg cgagcagcag cgcgaaggcc gccagcgcca gccagcgcca gatcggaaaa
     3361 tagccaaagc cccatcgttg cagggccggc agcggcgaag cggccgccgt ttttaaatcg
     3421 ccgccctccg cgtccagcgg cgcaatgacg gctgctgaat cgcgggaatc ccgtgtgacc
     3481 caatccagca ggttttgcag caacaccgga aagctggcgc gcagcggcag gtcggatttt
     3541 tcgggcggaa aaagcagcgc ggcccagcgg acctcctccg ccgcccgcgc gatgagcagc
     3601 ggcccctggg tgcccgtcac gacttcctcg tagcccgaac cggcgggctg gccggcctgc
     3661 tcgccgatcc gccagtgccc gggattgagc gagcggagca gcggatgatg cgaatcgaac
     3721 ttcacgaccg ccggattggc ggcggttccg ggcgtgaggc caggggcggt tcccagaaat
     3781 ccaaagcgcc ctttttcgag gaagttgccg gggatttggc catcgaacag cgtgacgtcg
     3841 aaggcttcgg acaatgcggg attccattcg cccggccgca gcatttcaaa ttggagcgac
     3901 tcgtccgcgc ggagcgcttt ttccacaaac caagtgccct cggagaccag cagcacgcgg
     3961 cgcggcggtg gattcggcac ggtgagctgg acgctttgat ccgcgtcgag cgcatccttc
     4021 gcgcgaaggc gggcgaggta gggttccgat ccgaccggga agtcgaagtg caaatcccgc
     4081 ggctcgcccg ggttgagccg cagccgccgg gtatcgagca atcgccccgc ctgttcgagg
     4141 tcgagctgaa cttcgcgggg cgcgttcgca tgggaaacgg gccgcaaaaa aagcagcgcc
     4201 cggccatttg cctcccgcgc gccggcggcg gcgaaccccc aattttccgc cggatttccg
     4261 aacgtcacga aatgaacgcc gcgctcgctt tccgccggtg gacgatcggt caatacctga
     4321 acttccgccg ccggccccgc cgccgcggcg atctcgcggg cgaattgaat ggcggccgga
     4381 agatcgccat cgcccggggt ggcgggcaaa tgtttcagcg cctcgcgcag cgcccggggg
     4441 tcgcttgaaa acggatgaat cagccgcggg gagccgtccg ctgtccccag cgccaccagg
     4501 gcgattggcg aatcctcgcg cgccatttcc gcggcggcct cggcaaagcg gcgcgccttg
     4561 gccaaggcgg attgatgcag cccatccacg gcctgcatgc ggtcccgcgt atccaggacg
     4621 atcacccgcg cggcggcggg ggaacggtcc gcttccggcc gggagacggc gagaatcagg
     4681 aggagcaaaa tgagcaattg caacagcagc gaaagccagg cgcgaatgcg gccccatccc
     4741 cgcgcgcggg cgttgagccg cagaacctgt tcccacagca aaagactcgg cacgcgctgc
     4801 tccggcggcc gcacgcgccg caggtaaagg ccggccgcca cgggaagggc cgccagcaga
     4861 aataggccgg cgggaagggc aaacgtcatt tcaacaaccc tccgcgcaat tcccgcagga
     4921 gcacgtcctc gaatcgttcg tcgctgcgag cccggtgcat ggccatccgg cgtttccggc
     4981 caaaggcgtc cagttccgcc gagaatgccg ccacgcgccg ggagtaggcc tggagcaggg
     5041 cgggagtcgc cacgatccgc tcgccgcggc cggtctcgct gtcgcgcagg atccagtccc
     5101 cgcgcagcgg cgacaggatt tcggcgggat cgagcaaatg gatgagcgcc acctcgaacc
     5161 ggctggcggc caggcggcg
//
LOCUS       DATDAY010000431         5315 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_259934, whole genome shotgun sequence.
ACCESSION   DATDAY010000431 DATDAY010000000
VERSION     DATDAY010000431.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 5315)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5315)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5315
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_259934"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..645)
                     /locus_tag="VIS74_08535"
     CDS             complement(<1..645)
                     /locus_tag="VIS74_08535"
                     /inference="COORDINATES: protein motif:HMM:NF015585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HEY5793332.1"
                     /translation="MFKFISPRAAALLMGGLLAAIANSHSADSLPPADVYLFGGQSNS
                     AGFGMGKETLPPELQTTFQKVQIWTPGANERKGLLLPLQPGVNSNHPNAMWAAESPLA
                     LLLEKDPKQAGPIFFIKSAIGGAPLQPRENHKDWHPEKGGYYGGFRDGMLRAVEHIKT
                     AGYEPRFRAFVWVQGEAEIGEGPAVAAQYSVWLKDLINAVRRDTGVPDLNVVVVR"
     gene            906..1478
                     /locus_tag="VIS74_08540"
     CDS             906..1478
                     /locus_tag="VIS74_08540"
                     /inference="COORDINATES: protein motif:HMM:NF014116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate binding domain-containing protein"
                     /protein_id="HEY5793333.1"
                     /translation="MNRPTPQNPSMCARVLLASLLISGSLFANILPAGTFELTEVRGQ
                     PQFDGWNVSPGFDAEGVSAIGRVTPGISPKGTGSLVLEFQKGGVYRVSPAARIDTIPI
                     NPGQKYRLSFQVKAEGGPINLEALIVEADALKKTTPAAVNSVETTAEWKSQSVEWQAG
                     ADKQFMSFTLQFGGKAPGTAQLADVSIEEI"
     gene            1585..2649
                     /locus_tag="VIS74_08545"
     CDS             1585..2649
                     /locus_tag="VIS74_08545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEY5793334.1"
                     /translation="MSEAIEENYDVVPEPPAPEAAASPDLDAVQFCRSAYQEVQREIS
                     KVVVGQTSVVEEILIAILTRSHALLVGVPGLAKTLLISTLAKTMQMSFKRIQFTPDLM
                     PSDITGTEVIYQDPVSGHKEFKFLPGPLFANIVLADEINRTPPKTQAAMLEAMQERRV
                     TVGGTTHKLPEPFFVLATQNPLEQEGTYPLPEAQLDRFMFLVQVDYPSEEDELLVMKM
                     GTGMKGEEPQPVLNAEHVLYIQETVKSIPVADHVYRYAQKIVRCTRPKTPEALDFCRQ
                     YLSFGAGPRASLSLIMAAKAHAMVNGQIYAGCANVAAVTPSILRHRLAPNFAAQSEGI
                     TTDDIVARILQTIPQHDPAA"
     gene            2670..3566
                     /locus_tag="VIS74_08550"
     CDS             2670..3566
                     /locus_tag="VIS74_08550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEY5793335.1"
                     /translation="MAESSLLDPETVSRGEALGLMARRVVEGYRVGEHRSPFRGFAIE
                     FAQHREYTTGDDIRHLDWKVLGRSDRYYIKQYEQDTNYVAHVVMDASRSMEYGSGKIT
                     KLHFAKALAACLAYLVLIQRDAVALALFDTDVREYLPRTDNLGRIHHLMNRLAAFQPN
                     DGTLLGASLEQVAGRARSRGIVTIVSDLLDDEEGLAQGLRRLTFAGHEVIVFHVLDPY
                     ELTFPFTGTWKLEGLEDATEFQTSPADIRKTYLERFDAYRARVRQICEKFNTHYVLAD
                     TGKSLAEVLSGYLAFRRAVGRK"
     gene            3573..>5315
                     /locus_tag="VIS74_08555"
     CDS             3573..>5315
                     /locus_tag="VIS74_08555"
                     /inference="COORDINATES: protein motif:HMM:NF024909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatA domain-containing protein"
                     /protein_id="HEY5793336.1"
                     /translation="MSFLNPVVLFGLAAISVPVAIHLLNKFRVQRTRWAAMRFVQESL
                     RRNRRQIQLEDLLLLLLRCLLVALLVFAFARPALKTLLAPAAGGSGPAATAVVLDHSA
                     SMGQSDGVETRFEQGKKAARQLLDQLEPGSRAALFLASNHSEALIAQPTTDFARFRRS
                     LELAPLSEDASDLSQSIRSAFNALAPLAGHRREVVVYTDSQTSAWNRIEEIRQIQREH
                     PDIQLRPVILGAKGEENLAIVGLQAKGGVPAIGQPSRFQIDVANFGAAPVEGARLTIA
                     SDGGAPADETLIPRIAPGQTQRAHLFVRFSTPGYHTVTAATSPDRLPVDNQRTAAVQV
                     VDRTRALLVENNPQSEPFEQDGYFLANALAPVAADRLDQYYLKVGATSFARLGSVDLS
                     ANDLIFLCNPGPLSDETAARLEKYVQAGGSLVIFPGPKMDPAKWQATPALARLLPATL
                     EPARDAGGATPLSWQTDNFEHPVTAIWNDRSEGTLSSVRFTRYFPLKLKPAAEKSPAG
                     KPEVIVRFANGEPSVVEAAYGQGNVVLFNSTVTPQWNNLPLHPGFVSLMQRLMGYLHR
                     EQATRLALAPGETFE"
BASE COUNT          993 a         1863 c         1532 g          927 t
ORIGIN      
        1 gcggacaacg accacgttca gatcgggaac cccggtgtcc cggcgcacgg cgttaatgag
       61 atccttgagc cagacactgt attgcgcggc cacggccggg ccttccccga tctccgcctc
      121 gccctgcacc cagacaaagg cgcggaaacg cggctcgtaa cccgcggtct tgatgtgctc
      181 caccgcccgg agcatgccat cgcggaaacc gccgtaataa ccgccctttt cgggatgcca
      241 gtccttgtga ttctcccgcg gctgaagcgg cgcgccgccg atggcggact tgatgaagaa
      301 aatcggcccg gcttgcttcg gatccttttc caagagcagc gccagcgggg attccgccgc
      361 ccacatggcg ttgggatggt tgctgttcac gcccggttgc aggggcagca gcagcccttt
      421 gcgctcgttg gcgccgggcg tccagatctg gactttttgg aacgtcgtct gcaactccgg
      481 cggcagggtt tccttgccca tgccgaagcc ggcggaattg ctctgcccgc cgaagagata
      541 gacgtccgct ggcgggaggg aatccgcgga gtgggagttg gcgattgcgg caagcagtcc
      601 gcccatcaac agggcggcgg cgcgggggga aatgaatttg aacattcggc tgagcggtaa
      661 actctaaaga agaaacgcag ccggttgatt tcgtcccgca atccatgcag gatttcctca
      721 aaaggcccgc cttttccccc actatcaaga caatcccccc tctcccgtca aagaaaatga
      781 ttcgtttctt tcctccgcta ttccgccgcc ccggggaccg cccgccggcc tgtggttata
      841 ctaatataat aatattgacg ctgaacccgg cccgtggaag gtttcagcga tttccccctc
      901 acgcaatgaa ccgccccacc ccgcagaatc cgtcaatgtg cgcgcgggtt cttttggcca
      961 gcctgctgat ttccggatcc ctctttgcga atattctgcc cgccggcacg ttcgaactca
     1021 ccgaagtgcg gggacagccc cagttcgacg gctggaatgt ttccccgggc tttgatgcgg
     1081 agggcgtttc cgccattggc cgcgtgacgc cgggaatcag cccgaagggg accggttcgc
     1141 tcgtgctcga attccaaaag ggcggcgttt accgcgtgag ccccgcggcg cgcattgaca
     1201 cgatccccat taacccgggg caaaaatacc ggctctcctt tcaggtcaaa gccgagggag
     1261 ggcccatcaa cctcgaagcg ctgatcgtcg aagccgacgc cttgaaaaaa acgactcccg
     1321 cagccgtgaa ctcggtggaa acgaccgccg aatggaaatc ccaaagcgtg gaatggcagg
     1381 ccggcgccga caagcaattc atgtccttca ccctccaatt tggcggcaag gcgccgggca
     1441 ccgcccagtt ggcggacgtc tcgattgagg aaatttagcg ccgcagaatg acatcgcctt
     1501 cgaacccatt ttttggcatc ctgcaaaacc agcccgctca ttgattcact ccaaagactc
     1561 gaccactcaa ccctgactcc catcatgtcc gaagcgattg aagaaaatta cgacgttgtt
     1621 cccgagccgc cggcgcccga agccgccgct tcgcccgact tggatgccgt gcaattctgc
     1681 cggtccgcgt atcaagaggt gcagcgggaa atttccaagg tggtggtcgg gcagacctcg
     1741 gtcgtggagg aaatcctgat cgccattctc acgcggagcc acgccctgct cgtcggcgtg
     1801 ccgggactgg ccaagacgct gctcatttcg acgctggcga agacgatgca gatgtcgttc
     1861 aagcgcattc aattcacgcc ggacttgatg ccgagcgaca tcaccggcac cgaggtgatt
     1921 taccaggatc ccgtctccgg gcacaaggag ttcaaattcc tgcccggccc gctgtttgcc
     1981 aacatcgtgc tcgcggacga aatcaaccga accccgccga agactcaggc ggccatgctc
     2041 gaggccatgc aggagcgccg cgtgaccgtc ggcggcacga cccacaagct gcccgaaccc
     2101 ttcttcgtgc tcgcgaccca aaacccgctt gagcaggaag gcacctatcc gttgcccgaa
     2161 gcgcagctcg accgcttcat gttcctcgtg caggtggatt acccgagcga ggaggacgaa
     2221 ttgctcgtga tgaagatggg caccggcatg aagggcgagg agccgcagcc ggttctcaac
     2281 gccgagcacg tgctctacat ccaggaaacc gtgaagtcca ttccggtggc ggatcacgtc
     2341 tatcgctacg cgcagaagat cgtccgctgc acgcggccga aaacgccgga agcgctggat
     2401 ttctgccggc agtatctcag ctttggcgcc ggcccgcgcg ccagcctcag cctcatcatg
     2461 gcggccaagg cccacgccat ggtgaacggc cagatttacg ccggttgcgc caatgtcgcc
     2521 gcggtcaccc cctccatcct gcgccaccgc ctcgcgccga actttgccgc ccagagcgaa
     2581 ggcatcacca cggacgacat cgtcgcccga atccttcaaa ccattcccca gcacgatccg
     2641 gcggcctgac ccgcctgccc ggcactccca tggcggaaag ctccctgctc gatccggaaa
     2701 cggtctcccg cggcgaagcc cttggcttga tggccagacg ggtggtcgaa ggctaccgcg
     2761 tcggcgaaca ccgttcgccc ttccgcggtt tcgccatcga attcgcccag caccgcgaat
     2821 acaccaccgg cgacgacatc cggcacctgg actggaaggt cctcgggcgc tccgaccgtt
     2881 attacatcaa acaatacgag caggacacca actacgtggc ccacgtggtc atggacgcaa
     2941 gccgctcgat ggaatacggc tccggcaaaa tcaccaagct gcatttcgcc aaggcgctgg
     3001 ccgcctgcct cgcttatctc gtcctgatcc agcgcgacgc ggtggccctg gccctcttcg
     3061 acaccgacgt gcgcgaatac ctgccgcgca ccgacaacct cgggcggatc catcacctca
     3121 tgaaccgtct cgcggctttc cagcccaacg acggaaccct gctgggcgcc tcgctggagc
     3181 aggttgccgg ccgggcgcgc agccggggga ttgtcaccat cgtcagcgac ctgctcgacg
     3241 atgaggaagg cctggcccag gggctgcggc ggttgacctt cgccggccat gaagtcatcg
     3301 tctttcacgt cctcgatccc tacgagctga ccttcccctt caccggcacc tggaaattgg
     3361 aaggactcga ggacgccacc gaattccaga cctccccggc ggacatccgc aaaacttatc
     3421 tcgaacggtt cgatgcctat cgcgcgcggg tgcgccagat ctgcgagaag tttaacaccc
     3481 actacgtgct cgcggacacc ggcaaaagcc tcgccgaagt gctgagcggt tacctcgcct
     3541 tccgccgggc ggtcggccgg aaataatccc gcatgagttt tctgaatcca gtcgttttgt
     3601 tcgggctggc ggccatttcg gtgcccgtgg cgatccattt gctcaacaaa ttccgcgtgc
     3661 agcggacccg ctgggcggcc atgcgcttcg ttcaggaaag cctgcggcgc aaccggcgcc
     3721 agatccagct cgaggacctg ctcctgctcc tgctgcgctg cctgctggtg gcgctgctgg
     3781 tgttcgcctt cgcgcggccc gcgctcaaaa ccctgctcgc cccggccgcc gggggcagcg
     3841 gccccgccgc caccgcggtc gtgctcgatc attccgccag catgggacag agcgatggcg
     3901 tggaaacgcg cttcgagcag gggaaaaagg ccgcccgcca attgctcgac cagctcgagc
     3961 ccggatcgcg cgccgccctg tttctggcgt ccaatcactc cgaggcattg atcgcccagc
     4021 ccaccaccga cttcgcccgc ttccggcgca gcctcgaact cgcgccgttg agcgaggacg
     4081 ccagcgatct ttcccaatcc atccgctccg ccttcaacgc cctggcgccc ctggccggcc
     4141 accgccgcga agtggtggtc tataccgaca gccagacctc ggcgtggaac cggatcgagg
     4201 agattcgcca gattcagcgg gagcatcccg acatccagct ccgtcccgtg atcctcggcg
     4261 ccaagggcga ggaaaatctc gcgatcgtcg gcctccaggc caagggcggc gtgccggcga
     4321 tcggccagcc cagccgtttc cagatcgacg tcgccaattt cggcgccgcg ccggtggagg
     4381 gggcgcgcct gaccatcgcc tccgacggcg gggcgcccgc cgatgaaacc ctgatcccgc
     4441 gcatcgcccc cggccagacg caaagggcgc atcttttcgt gcggttttcc acccccggtt
     4501 accacaccgt caccgcggcg acttcgcccg accggctgcc ggtggacaac cagcgcaccg
     4561 ccgccgtgca ggtcgtggac cggacgcgcg cgctcctcgt ggaaaacaac ccccagtccg
     4621 aacccttcga acaggacggg tatttcctgg ccaacgcgct cgcgcccgtc gccgccgacc
     4681 ggctggatca atattacctc aaggtcggcg ccacctcgtt cgcccggctg ggctcggtcg
     4741 atttgtccgc caatgacctc attttcctct gcaaccccgg ccccctttcc gacgagaccg
     4801 ccgcgcggct cgaaaaatac gtccaggccg gcggcagcct cgtgattttc cccgggccga
     4861 aaatggatcc cgcgaaatgg caggcaaccc ccgccctggc gcgcctgctt ccggccaccc
     4921 tggagccggc gagggatgcc ggcggcgcca cgccgctgag ctggcaaacc gacaatttcg
     4981 aacaccccgt gaccgccatc tggaacgacc gctccgaagg cacgctctcg agcgtgcgat
     5041 tcacgcgcta ttttccgctg aaactcaaac cggccgcgga aaaatccccc gccgggaaac
     5101 ccgaggtcat cgtgcgtttt gcgaatggcg aaccttccgt cgtggaagcc gcctatggac
     5161 agggaaacgt cgtgctcttc aacagcaccg tgacgccgca gtggaacaac ctcccgctgc
     5221 atcccggctt cgtctcgctc atgcagcgcc tcatgggtta cctccaccgc gagcaggcca
     5281 cccgcctcgc cctcgcgccg ggcgaaacct tcgaa
//
LOCUS       DATDAY010000432         3209 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_1935294, whole genome shotgun sequence.
ACCESSION   DATDAY010000432 DATDAY010000000
VERSION     DATDAY010000432.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 3209)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3209)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3209
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1935294"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..464
                     /locus_tag="VIS74_08560"
     CDS             <1..464
                     /locus_tag="VIS74_08560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5793337.1"
                     /translation="VPPGGIQPPKPPSLPGLKPAVAPGFKPPTIPPIPGVGAPKLLGS
                     VAPVAGGVPTAPRPPSLSAAPVVAPAPLKADAKKETAKVPPTVSPKPGLPQATVQLSA
                     KPDPSKSVSASAIKIATAPAPAAGSELSPALGGLAAAAALIALAIQVWMLL"
     gene            612..941
                     /gene="trxA"
                     /locus_tag="VIS74_08565"
     CDS             612..941
                     /gene="trxA"
                     /locus_tag="VIS74_08565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEY5793338.1"
                     /translation="MASEHVVELTEANFDSEVKQSAVPVLVDFWAPWCGPCKMIGPVV
                     EEIAAEKAGAFKVGKINVDDQPGLAQQFRINAIPALYVFKGGEVKDKVIGMTSKANLI
                     AKLEGAA"
     gene            975..2159
                     /gene="carA"
                     /locus_tag="VIS74_08570"
     CDS             975..2159
                     /gene="carA"
                     /locus_tag="VIS74_08570"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003280874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEY5793339.1"
                     /translation="MSQEALLALADGRVFRGQAFGATATVTGEVCFNTSMTGYQEVLT
                     DPSYRGQIVAMTAPQIGNYGVNVLDEESAEPHVRGFIIEELSPVASNWRSQEDLADYL
                     TRGGIPGIEGIDTRALTRHLRTLGATNGCLTTEGLSEAEALRRARDCAGVAGMDFVKE
                     VTPVQAYDWDSGDVLSAPWQLPQSAGKPPVHADQQGNQFGPLPPVRNRIVAFDFGMKK
                     NILRNLRRQGFQVRVVPATTSAREVLAQEPDGVFLSNGPGDPAALDYVHETVRELVGK
                     TPIFGICLGHQILAYAFGGKTFKMKFGHRGANQPVKDLRSGKISITSQNHGFAVDPES
                     LPADVAVTHVNLNDGTVAGMEHKTHPVFSVQYHPEAAPGPHDADYFFEQFAKVIDAEK
                     AK"
     gene            2172..2687
                     /locus_tag="VIS74_08575"
     CDS             2172..2687
                     /locus_tag="VIS74_08575"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEY5793340.1"
                     /translation="MPKLQIFLAEESPSIHDLPEEKVTVGRLPDNTLQISDESVSSHH
                     AELTFEQGLYHLRDLGSTNGTFVNGEMVTDVILQEGDQIRFGKVESFFAADATTTSFQ
                     PPPETKDLSAEAGEASSRPENFLNTSPFPKEVRERDPLALAATVLAVIGVLGFAGSLY
                     GIYLLMQAPGV"
     gene            2735..>3209
                     /locus_tag="VIS74_08580"
     CDS             2735..>3209
                     /locus_tag="VIS74_08580"
                     /inference="COORDINATES: protein motif:HMM:NF025729.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4412 domain-containing protein"
                     /protein_id="HEY5793341.1"
                     /translation="MKSSIVLLLSLAAAACCHAGFEMVQEINQSGPQGTLTMQTVTKA
                     EGDMMRVDAGKEVTTLINTKTKEITTLMHRQKAYMQVPASVIENAEKMAREQEAKQGE
                     GAVPVATGKTQEINGYQCAEYTQTVNGNKMAYWLTKDLQGADALMAQAKGMGTFDP"
BASE COUNT          639 a          972 c         1002 g          596 t
ORIGIN      
        1 cggttcctcc cggcggcatc cagccgccca agccgccgtc ccttcccggg ttgaaaccgg
       61 cagtcgctcc gggatttaag ccgcccacca tcccgcccat tccaggggtg ggggcgccca
      121 agctgctggg ctccgtggct ccagtggctg gcggggtgcc gaccgcgccc cggccgcctt
      181 ccctttccgc cgcgccggtg gtggctcccg ctccgctcaa agcggacgcc aagaaagaaa
      241 cggccaaggt tccgccaacc gtcagcccga aacccggcct gccgcaggcg accgtgcagc
      301 tttcggccaa acccgatccc tccaagtcgg tttccgccag cgccatcaaa atcgcgacgg
      361 ctcccgctcc ggcggcggga agcgagctca gtccagccct tggcggactc gcggcggcgg
      421 cggccctgat cgccttggcg atccaggtct ggatgctttt gtaggcgcca gttctttcaa
      481 aagcgggctt tgcttcggcg aggcccgctt tttatttgtc ccgaaaggac ggaatggccg
      541 cgcgcgcaat tctttcaacc cggctgcggt gggatttgct ttcttggggg caatccgtgt
      601 agattcgggc catggctagc gaacatgtcg tcgaattaac ggaagcgaat tttgattcgg
      661 aggtgaagca atctgcggtg ccggtcctgg tggacttctg ggcgccgtgg tgcggaccct
      721 gcaaaatgat cggtccggtc gtggaggaaa tcgccgcgga gaaggcggga gccttcaagg
      781 tgggcaagat caatgtggat gaccagccgg gactcgccca gcagttccgc atcaatgcga
      841 ttcccgcgct gtatgtgttc aagggcggcg aggtgaagga caaggtcatc ggaatgacca
      901 gcaaggccaa tctcatcgcc aagctcgaag gcgcggccta aggcgctgga agctcggcga
      961 attttttctc cctcatgtcc caggaagctc tccttgccct cgcggacggg cgcgtgttcc
     1021 gcggccaggc ctttggcgcc acggccacgg tcaccggcga agtttgtttt aacacctcca
     1081 tgacgggtta tcaggaggtg ctgacggatc cgtcctatcg cggccagatc gtggcgatga
     1141 ccgcgccgca aatcggcaat tatggggtca atgtcctcga cgaagagagc gcggagccgc
     1201 acgtgcgcgg ttttatcatc gaggagctca gcccggtggc gagcaactgg cgctcgcagg
     1261 aggatttggc ggattacctg acgcgcggcg gcattcccgg catcgaagga attgacacgc
     1321 gcgcgctgac gcggcatctg cggacgctcg gcgccaccaa cggatgcctg acgaccgagg
     1381 ggctttccga ggcggaggcc cttcgccgcg cgcgggattg cgccggggtg gccggcatgg
     1441 attttgtgaa ggaggtgact ccggtgcagg cgtatgattg ggacagcgga gacgtgctta
     1501 gcgcgccctg gcagctgccc cagagcgctg gtaaaccgcc ggtccacgcg gatcagcagg
     1561 gcaaccagtt cgggccgctg ccgcccgtgc ggaatcgaat tgtcgctttt gattttggaa
     1621 tgaagaaaaa catcctgcgc aaccttcggc ggcaggggtt tcaagtccgg gtcgtacccg
     1681 ccaccaccag cgcgcgggaa gtgcttgccc aggagccgga cggtgtcttt ctgagcaacg
     1741 gcccgggcga tcccgccgcc ctggattacg tgcatgaaac ggtccgggag ctcgtgggca
     1801 aaacgccgat ctttggcatc tgcctcggcc accagattct ggcctacgcc tttggcggga
     1861 agacgttcaa gatgaagttc ggccatcgcg gcgccaatca gccggtgaag gatctccgca
     1921 gcggaaaaat ttccattacc tcgcaaaatc atggctttgc ggtggatccg gagtcgctgc
     1981 cggcggacgt cgcggtcacc cacgtgaatc tgaacgacgg cacggtggcg ggcatggagc
     2041 ataaaaccca tccggttttc agcgtgcaat accatccgga agccgcgccc ggcccccacg
     2101 atgcggatta ctttttcgaa cagtttgcca aagtcattga tgccgagaag gcgaagtgat
     2161 aggaaaaaac catgccgaag ctgcagattt ttttagcgga ggaatccccc agtattcacg
     2221 accttcccga ggaaaaagtc acggtcgggc gcctgcccga caacacgctg caaatctcgg
     2281 atgaatccgt ttccagtcat cacgccgagc tgacgttcga acaggggctc taccacctgc
     2341 gcgatctggg ctcgaccaac ggcacattcg tcaatggcga gatggtgacg gacgtcatat
     2401 tgcaggaagg ggatcagatt cgttttggaa aggtggagag cttttttgcc gcggatgcca
     2461 caacgacgag ctttcagccg cccccggaga caaaagacct ctccgccgag gccggcgagg
     2521 ccagctcgcg cccggaaaat tttctgaaca cctcgccgtt tcccaaggaa gtccgggagc
     2581 gcgacccatt ggcgcttgcc gcgaccgtgc tggccgttat cggcgtgctc ggatttgccg
     2641 gttcgcttta tggcatttat ctgctgatgc aggccccggg ggtctgacgg ccattttccc
     2701 ccctccatct ctttttcgct atcttttcca tcccatgaaa tcatccatcg tcctcctcct
     2761 tagtctcgcg gccgccgcct gttgccacgc gggttttgaa atggttcaag agatcaacca
     2821 gagcggcccg cagggaacgc tgaccatgca gaccgtcacc aaggccgaag gcgacatgat
     2881 gcgggtggac gcgggaaaag aggtaacgac gctcatcaac accaagacga aggaaatcac
     2941 caccctcatg caccgccaga aggcctacat gcaggttccg gcctccgtga ttgaaaacgc
     3001 cgaaaaaatg gcccgggagc aggaggccaa gcagggggaa ggcgcggtgc cggtggccac
     3061 gggcaaaacg caggaaatca atggctacca atgcgcggaa tatacccaga ccgtgaacgg
     3121 aaacaagatg gcctattggc tgacgaagga tctccaggga gcggatgccc tgatggctca
     3181 agccaaggga atgggaactt tcgatccct
//
LOCUS       DATDAY010000433         2461 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_1754065, whole genome shotgun sequence.
ACCESSION   DATDAY010000433 DATDAY010000000
VERSION     DATDAY010000433.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2461)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2461)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2461
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1754065"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..615)
                     /locus_tag="VIS74_08585"
     CDS             complement(<1..615)
                     /locus_tag="VIS74_08585"
                     /inference="COORDINATES: protein motif:HMM:NF028500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5793342.1"
                     /translation="MPSASFVVKAAVLLPAATPLAEMDWIPHAKLTPLARLGLVTLGD
                     LVQHFPRRHEDRVRFDQFPEGETPRAVCLLGTVVKTAAKRYGGWRKAFEATLESGDSV
                     LSQRLMCRWFNAHFVQKMIATGQRLVVYGKAKSRGKTLVIDHPEFEIVEHDEEESIHF
                     NRLTPVHPAGEGVTVRALRYWIWRALEVVDWATLASRIPIVEALV"
     gene            complement(635..2323)
                     /gene="recJ"
                     /locus_tag="VIS74_08590"
     CDS             complement(635..2323)
                     /gene="recJ"
                     /locus_tag="VIS74_08590"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HEY5793343.1"
                     /translation="MHYRWILPGPASRSLAARLAAEFRVSPLAGSVLARQGLVDSADI
                     EPFLRPLLKQLSDPFQLPEMRAAVERIDLALRHKERIVLYGDYDVDGVASLAVLGRLL
                     RAYGGNVECFLPHRIDEGYGLSAAGVERCFEEWSPQLLIAVDCGTNSVAEVAEMRRRG
                     AEVIIVDHHEYAGTRPDCVALVNPKLKGEFTYLCSAGLAFKVAHALLKHSPLPDFDLR
                     EMLDLVALATLADLVPLVAENRLLVRKGLEQMRVTRWPGLAALMVEAGVKTPVRSSDV
                     GFQLGPRINAAGRLGTAQAALRLLLTNDRAEAAQLAAQLNRQNAERRGVEQLVVREAE
                     AWVRDHFDPRHNASIVAGQRDWHDGVLGIVASRLSRRHYRPTLIVGFNAEGSGKGSGR
                     SIDGLCLVTALTRCGAHLEKFGGHEMAAGLSLREENFPVFREAFEQAAREMLSDEMLI
                     PSLSIDAEMKLDDVDFPAIDDQDLLEPYGTGNRQPLCLVRGVTPSGAPRVLKEKHLRF
                     DFQHGRRRIVAIFFDGASQELPRPPWDIAFRLERNEFNGRVDPQMQIVAIRAAA"
BASE COUNT          445 a          828 c          818 g          370 t
ORIGIN      
        1 cacaagcgcc tctacaatgg gaatccgcga cgccagcgtc gcccaatcca cgacctccaa
       61 cgcccgccag atccaatacc gaagcgcccg caccgtcacc ccctcgcccg cgggatgcac
      121 cggcgtgagg cggttgaaat ggatggattc ctcctcgtcg tgctcgacga tttcgaattc
      181 cggatggtcg atgaccagcg tcttgccgcg cgacttggcc ttgccgtaaa ccaccaagcg
      241 ctggccggtg gcgatcattt tctgcacaaa atgcgcgttg aaccaccggc acatcaaccg
      301 ctggctcagc accgaatccc cgctctccag cgtggcttca aacgccttgc gccagccgcc
      361 gtagcgcttc gcggccgtct tgacgaccgt gccgagcagg cagaccgcgc ggggggtctc
      421 gccctcgggg aattgatcga accggacgcg gtcctcgtgg cggcggggaa aatgctgcac
      481 gaggtcgccg agcgtcacca ggcccaggcg cgcgagcggg gtgagcttgg catggggaat
      541 ccaatccatc tcggcaagcg gagtcgcggc aggcaggagc acggctgctt ttaccacgaa
      601 ggaggcggag ggcacgacgg atttgcggga aacctcacgc cgcggcgcgg atggccacga
      661 tctgcatctg cgggtccacc cggccgttga attcatttcg ctccaaacga aaggcgatgt
      721 cccacggcgg ccgcggcaat tcttggctgg cgccgtcgaa aaagatcgcc acgatgcggc
      781 ggcggccgtg ctggaaatca aagcgcaggt gtttttcctt cagcacgcgc ggcgcgccgg
      841 acggcgtcac gccgcgcacc aggcaaagcg gctgccggtt gccggtgccg tagggctcga
      901 gcaggtcctg gtcgtcgatc gccggaaaat ccacgtcgtc cagcttcatc tcggcgtcga
      961 tcgacaggga gggaatcagc atttcgtcgc tgagcatttc ccgcgcggcc tgctcgaagg
     1021 cctcgcggaa aaccgggaaa ttctcctcgc gcagggaaag gccggccgcc atttcgtggc
     1081 cgccgaattt ctccagatgc gcgccgcagc gcgtgagcgc ggtcaccagg cacaggccgt
     1141 cgatgctgcg cccgctgccc ttgccgctgc cctcggcgtt gaatccgacg atcagggtcg
     1201 ggcgataatg ccgccgcgaa agccgcgagg cgacaatgcc cagcacgccg tcgtgccaat
     1261 cccgctggcc ggcgacgatg ctcgcattgt ggcggggatc gaaatgatcg cgcacccagg
     1321 cctcggcctc gcgcaccacc agctgctcga cgccgcggcg ctcggcgttt tggcgattga
     1381 gctgcgcggc gagctgggcc gcttcggcgc ggtcattggt cagcagcagg cgcagggcgg
     1441 cctgcgcggt gccgagccgg ccggcggcat tgatgcgcgg accgagctgg aatcccacgt
     1501 cgctgctgcg aacgggcgtt ttcacgccgg cttcgaccat caaagccgcg aggccgggcc
     1561 agcgggtgac gcgcatttgc tccagtccct tgcggacgag caggcggttt tcggcgacca
     1621 gcggcacaag atcggccagc gtggctagcg cgacgaggtc gagcatttcc cgcagatcga
     1681 aatccggcag cggcgaatgc ttgagcaggg catgcgcgac cttgaaggcc aggccggcgc
     1741 tgcagagata cgtgaactcg cccttgagct tgggattgac cagcgccacg caatccggcc
     1801 gcgtcccggc gtattcgtga tggtccacga tgatgacctc cgcgccgcgc cggcgcatct
     1861 ccgcgacttc ggccacggaa ttcgtcccgc aatccacggc gatcaacaat tgcggcgacc
     1921 attcctcgaa gcaccgctcc acgccggcgg cgctcagccc gtaaccctcg tcgatgcgat
     1981 gcggcagaaa gcattccacg tttcccccat aggcccgcag caggcggccg aggacggcga
     2041 gcgacgcgac gccatccacg tcgtaatccc cgtagagcac gatgcgttcc ttgtggcgca
     2101 gggcgagatc gatccgctcc accgcggccc gcatctcggg caattgaaaa gggtcggaaa
     2161 gctgcttgag cagggggcga agaaacggct cgatgtcggc ggaatccacg agcccctgcc
     2221 gggccagcac gctgccggcc agcggggaaa cccggaattc ggcggcgagc cgggcggcca
     2281 gcgaacgtga ggccgggccg ggtagaatcc accgataatg catgggcgaa agctagaagc
     2341 tccggggaag gcagaaaagc aggaaatcgg acaatccgcc gcccgcgccg gccggagcgc
     2401 tcgagccgct tcgattcagt agccgcggcg agcggcggcg ttggcaacgc cgccccaccg
     2461 g
//
LOCUS       DATDAY010000434         3071 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_1946802, whole genome shotgun sequence.
ACCESSION   DATDAY010000434 DATDAY010000000
VERSION     DATDAY010000434.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 3071)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3071)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3071
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1946802"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..709)
                     /locus_tag="VIS74_08595"
     CDS             complement(<1..709)
                     /locus_tag="VIS74_08595"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEY5793344.1"
                     /translation="MTHAVSSDESPRADSGFASRIRTTCPRDCYDSCGIVADRRTGRL
                     RILGDPDHAIARGALCQKCAVAYNSVWLDPARRLRHPLRRVGAKGEGRFEAVSWEEAI
                     ADIAGRLKALAVRGEARRIIQTHYTGTFSWIAFWFPWRFFNRLGCTEVDPDTICNKAG
                     HVALNYVLGDSLMGFDPRQARAAKAILIWGANPSHSAPHLHEHWLGEFAGPKIVIDPI
                     RHATAEKADLHLQLHPGS"
     gene            complement(967..1923)
                     /locus_tag="VIS74_08600"
     CDS             complement(967..1923)
                     /locus_tag="VIS74_08600"
                     /inference="COORDINATES: protein motif:HMM:TIGR04267.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEXXH motif-containing putative peptide
                     modification protein"
                     /protein_id="HEY5793345.1"
                     /translation="MPLARALDQRMRDGLAASLQYLAEAGGAALLPDPAPFAAVIGAI
                     KSGLVSPLAFCLYSEIVQAVEAGRLSAARESLQELMALPKPGARTEIIDLADPGADLK
                     SRLYQRYVDTDPANRFPLFRPSSEKSAASRRLIEDAFVLMRDTDPDLHAEIQALLREI
                     VLAAGPEDGSGLTFDGASSFMMWGAIVINADRSDSVLEMVQMLAHESAHNVLFGLFSE
                     DPALFNGYSERQASPLRRDPRPLEGIYHAAFVTARMYRAVHHVARSAGASSPLREEAG
                     QALEELRGLFRQGYETVIAHGRFSPLGREVIEEASRYLEAHR"
     gene            1922..2302
                     /locus_tag="VIS74_08605"
     CDS             1922..2302
                     /locus_tag="VIS74_08605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5793346.1"
                     /translation="MPAAIEFITSQSPALSSLQEATERGLSLKENCGADLFAKPVPGF
                     SADFGVQARAGNPDFFAHLGFKAFGEIARGRGAFIFPGEAKTEQLRVVEADLLVHGAI
                     HSPWWRFVNPKTGSDLMNRALFRP"
     gene            2299..>3071
                     /locus_tag="VIS74_08610"
     CDS             2299..>3071
                     /locus_tag="VIS74_08610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5793347.1"
                     /translation="MIQFVLTQNHTYTLARVRKGSGMPAIGFIDYDALIGRSAIRRGT
                     YIFGDFDRLGHWDLELAGTIYQKLAKEPGFTLLNNPARALQRFNLLRLLHARGLNGFN
                     VHRAAECGPSMRFPVFLRKERGHGFPLSPLLNTLEDTEQAIEGALAQGIPLENLMLVE
                     YLAEPIRPGLYRKWAVFRIGDRLLPHLCVHDTQWLVKYGKKGIADEDSYRGEREMLAA
                     GSCIDETHLRKVFELAQIDYGRLDFGLVEGKIQVYEINT"
BASE COUNT          620 a          940 c          966 g          545 t
ORIGIN      
        1 cgctgccggg atgcaattgc aaatggagat cggccttttc cgccgtggcg tgccggatgg
       61 gatcaatgac gattttcgga cccgcgaact cccccagcca gtgctcgtgc aggtgggggg
      121 cggagtgcga gggattcgcc ccccagatca gaatcgcctt cgccgcgcgc gcctgccggg
      181 gatcgaagcc catgagggaa tccccgagca cgtaattgag cgccacgtgg ccggccttgt
      241 tgcaaatggt gtccggatcc acctcggtgc agccgaggcg attgaaaaac cgccatggga
      301 accagaaggc gatccaggaa aacgtgcccg tgtaatgcgt ctgaataatc cgccgcgcct
      361 cgccccgcac ggccagggcc ttcaggcgcc cggcgatgtc cgcgatcgcc tcctcccacg
      421 aaaccgcctc gaagcgcccc tcgcccttcg cgcccacccg gcgcagcgga tggcgcaggc
      481 gccgggccgg gtccagccac acgctgttgt aggccaccgc gcacttctgg cagagcgcgc
      541 cgcgggcgat ggcgtgatcc ggatcgccca gaatccgcag ccgcccggtg cggcgatccg
      601 ccacgatgcc gcaggaatca taacaatccc gcgggcacgt ggtccggatg cgggacgcaa
      661 acccggaatc cgcgcggggc gattcgtcac tcgaaacagc gtgggtcatg attgaataag
      721 cggcctccaa aaaacttttg tttcttttaa tgaaacactc cagccctgtc catgccggat
      781 tcgcccaaac ccgaaaatat tccagcggcc ggggaattcg gcaactttcg tcgggacgga
      841 gttttttaac cgcggatttg aaagcctggc cgccgcttcg cgtgcgttgc agcacggatg
      901 aacgcggata aggatgtgaa aatccgcgtc catccgcccc catgcgcgat ttaattccgg
      961 ttccccttaa cgatgggcct caaggtagcg ggaggcttct tcgatcactt cccggccgag
     1021 cggtgagaac cggccatggg caatgacggt ctcataaccc tgccggaaaa gaccccggag
     1081 ctcttccaat gcctgtccgg cttcttcccg aaggggggaa ctcgcgcccg cggaacgggc
     1141 gacgtgatgg accgcccgat acatccgggc ggtgacaaag gccgcatggt aaattccctc
     1201 gagcgggcgc gggtctctgc gcaaaggcga agcctggcgt tcgctgtatc cattaaagag
     1261 ggcgggatct tcggagaaca ggccgaagag gacgttgtgc gcgctttcat gggcaagcat
     1321 ctgcaccatc tcgaggacgg aatccgagcg atcggcattg atgacgatcg ccccccacat
     1381 catgaacgac gaagcgccgt cgaaggttag gccggagccg tcttcgggac ccgcggccaa
     1441 gacgatttcc cggaggagag cctgaatctc cgcgtgcaaa tccggatcgg tgtcccgcat
     1501 caggacgaag gcatcctcga tcaggcggcg gcttgccgcg gatttttccg aggaaggccg
     1561 gaagagcggg aaacgattcg cgggatcggt atcgacgtaa cgctgataaa ggcgggattt
     1621 cagatcggcg ccggggtctg ccagatcgat gatttccgtc cgcgcgccgg gcttgggcag
     1681 ggccatcagc tcctgaaggg attcgcgggc cgcggagagg cggccagcct cgaccgcctg
     1741 aacgatttcg ctgtagagac aaaaggccaa cggcgacacc agcccggatt tgatcgctcc
     1801 gatcacggca gcgaagggag ccgggtccgg caggagcgcg gcgccgcccg cctcggcgag
     1861 atactgaaga ctggcggcaa ggccgtcccg cattcgttga tccaaggcgc gggccaacgg
     1921 catgccggca gccatcgagt tcatcacaag ccagtccccg gcgttatcaa gtcttcagga
     1981 agcgaccgaa cgcggtcttt ccctcaaaga aaattgcggc gccgatctat ttgcaaaacc
     2041 agtccctggg tttagcgccg actttggcgt ccaagcccgg gccgggaacc cggacttttt
     2101 cgcccacctt ggcttcaagg cttttggcga aatcgcccga gggcgaggcg ccttcatatt
     2161 tccgggcgag gctaaaaccg agcaacttcg agtggtcgag gcggatttgc ttgtccatgg
     2221 cgccatccac tcgccatggt ggcgctttgt caatccaaaa accggctccg atctgatgaa
     2281 ccgcgcgcta tttcgcccat gatccagttc gtcctgacgc agaatcacac ctataccctg
     2341 gcaagggtgc ggaagggatc cggaatgccc gcaatcggct tcatcgacta cgacgccctg
     2401 atcgggagaa gcgcaatccg gcggggaact tacattttcg gcgactttga ccgattgggg
     2461 cactgggact tggagctggc gggaacgatt taccaaaaat tggcgaagga gcccggattc
     2521 acgcttttga acaatccggc ccgcgcgctc cagcgcttca acctgctgcg cctgctccat
     2581 gcccgggggc tcaatggctt caacgtgcat cgcgcggcgg aatgcggccc gtccatgcgg
     2641 ttcccggtct ttctgcggaa ggagcgcggg catggatttc cgttgagccc tttgctaaac
     2701 accctggagg acacggaaca ggccatcgaa ggcgcgctcg cgcaaggcat ccccttggag
     2761 aacctgatgc tggtcgaata cctggcggag ccgatccggc ccgggctcta tcgcaaatgg
     2821 gcggttttcc gcatcggcga ccggctcctt ccgcatctct gcgtgcatga cacgcagtgg
     2881 ctggtgaagt atggaaaaaa gggcatcgcc gacgaggact cgtatcgggg ggaacgggag
     2941 atgctcgcgg cgggctcctg catcgacgaa acgcacttga ggaaagtgtt cgagctcgcg
     3001 cagatcgact atgggcggct ggattttggc ctcgtggaag gaaaaatcca ggtgtacgag
     3061 atcaacacga a
//
LOCUS       DATDAY010000435         2050 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_1409187, whole genome shotgun sequence.
ACCESSION   DATDAY010000435 DATDAY010000000
VERSION     DATDAY010000435.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2050
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1409187"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(858..1925)
                     /locus_tag="VIS74_08615"
     CDS             complement(858..1925)
                     /locus_tag="VIS74_08615"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HEY5793348.1"
                     /translation="MQLDTTLRHATEALASTAGSPTGKWAEANPPGQALEEVSRIRTG
                     DEIEALAGDFTIMAGQLIRHQENLKEQIAAKTAEIQSDLDMARELQQAFLPREYPQVP
                     SRGEADPLTLHFHHLYQAAMSVSGDFCDVIKLDDHRAGVFIADVMGHGTRSALVTAIL
                     RTLLHNLAQEVNDPAWFLSQLNRHFHETMKQTDQLIFVTACYVVIDTQKKTVRCASAG
                     HPSPLCCNRLSGTTDLFFDSLKNNPAIGLLPHASYTSFTRRLHKGDIFLLFTDGVIEA
                     MNEQDEEFGQTRLLHCVENPQAHSLSVLTHGIVSAVKEFTGQSQLMDDLCLVAVEAAA
                     NPVHAPSPEAAEPAVSQSAAG"
BASE COUNT          408 a          590 c          648 g          404 t
ORIGIN      
        1 gccctcggcc ggcaatacct cgacccccgc ggacaaaaat tgaccctgca atccggcatg
       61 tccgtttccg tgaacatccg caccgccacc cgcagcgtgc tcagcctctt cttcaacttc
      121 ctcctgcagc cggtcgagaa actgaaggaa gtgcgctgag cacttgaatg gcggccacgc
      181 tcaccgcggg gaacacggtc aaaaagccgg cgttcttccg atgaggaaac ccataaactc
      241 accatggaga acaggaaggg catgaagaaa aaacaaagac gtttcaaccg cggagagcgc
      301 ggccaattgc agggatcgaa ggtcgccgga acggcaaacc gcctgtcccg cgaaagtgga
      361 acaggcggtc ttgcttcgct cggctgcggt tataagccct ttcgattcag tttgccggag
      421 tgagcggcgg cgttgccaac cccgccgggg ggatgcatcc cattagagcg atccttaaaa
      481 gaagcctccg aaatacccgg cgggttgagg cgctttgccg ggtggggaaa cggggacccc
      541 gcatccccat ttacgctggg cggttggcac aattgccgca cttcgtgcgc ccctcgggct
      601 gtcccgcttc cgtgggacag gctgtcttcg ctccggctgc ccctacagtg aatcgctttt
      661 tggaaatcgc ttttcggaaa tgctataaaa taccttcgat ctttttcttc atgcccttcc
      721 tgttctccat ggtaaaaaat ccatattcct ccgcgccttg gggtcgaatg tggcgccgac
      781 ggagcggcgc cctccatttt gcgccaatct tgatcgaaac cgttcgatag tccccatctt
      841 cgccgttcgt ttccgcttca acccgccgcc gattgggaaa ccgccggctc ggcggcctcg
      901 ggcgagggcg cgtgaaccgg attggccgcc gcctccaccg ccaccagaca gagatcgtcc
      961 atgagctggg attggccagt gaactccttc acggcggaga caatcccatg cgtcagcacg
     1021 gacaggctgt gggcctgggg attttccacg cagtgcagca ggcgcgtctg gccgaattcc
     1081 tcgtcctgtt cgttcatggc ttcgatgacg ccatcggtaa acaggaggaa gatgtccccc
     1141 ttgtgcagcc ggcgcgtgaa ggacgtgtag gaggcgtggg gcagcagccc gatcgcggga
     1201 ttgtttttca gcgaatcgaa gaagagatcg gtcgttccgg agaggcggtt gcagcacagc
     1261 gggctgggat gccccgccga cgcgcagcgg acggttttct tctgggtgtc gatcaccaca
     1321 taacacgccg tgacgaagat gagctggtcg gtttgcttca tcgtctcgtg gaaatgccgg
     1381 ttgagctggg aaaggaacca cgcgggatcg ttgacctcct gggccaggtt gtggagcagg
     1441 gttcggagaa tggcggtcac cagggcggaa cgggtgccat gccccatgac atcggcgatg
     1501 aagaccccag cgcgatggtc gtccagctta atcacatcgc aaaaatcccc gctgacggac
     1561 atggccgcct ggtagaggtg gtggaaatgc agcgtcagcg ggtcggcctc cccgcgggag
     1621 ggaacctggg gatattcgcg tggcaggaac gcctgctgga gttccctcgc catatcgagg
     1681 tcgctctgaa tctcggcggt tttggcggcg atctgctcct tcaggttttc ctgatggcgg
     1741 atcaattggc cggccatgat ggtgaaatcc ccggccagcg cctcgatttc gtcgccggtg
     1801 cgaatgcggg acacctcctc cagagcctgg ccgggagggt tggcttccgc ccatttcccc
     1861 gtgggggatc cggcggtgga cgccaatgcc tcggtggcgt ggcgcaaagt agtgtctaat
     1921 tgcaaaagac agatagatta agtaaagaga agggatgagt cgccgagcgc ccgaagtgtc
     1981 gtgttccgag gaagatcgca aagagcttga agggatgagt cgaagcggga gggaggaagc
     2041 gcggatggtg
//
LOCUS       DATDAY010000436         2897 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_702857, whole genome shotgun sequence.
ACCESSION   DATDAY010000436 DATDAY010000000
VERSION     DATDAY010000436.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2897)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2897)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2897
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_702857"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1568)
                     /locus_tag="VIS74_08620"
     CDS             complement(<1..1568)
                     /locus_tag="VIS74_08620"
                     /inference="COORDINATES: protein motif:HMM:NF015585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HEY5793349.1"
                     /translation="MKSYLARGCGLVLLGLAGTFSVQAEVSLPSIFSEHAVLQKSAKV
                     PVWGKAEPGEAVTVALDGTKAQTQAGADGKWRVALDLKDKAEGPFELIVEGKNKLVIP
                     DVLVGDVWVCSGQSNMEWVLKNTLDGDEEIAKSANPRFRQFCVKKNTSPEPLDTVAGR
                     WIVAAPETAEAFSAVGYYFGKQLNAELNKPIGLLHTAWGGTPSEAWTSAEALGKDPEL
                     KERMESLKAAIAAYPDQLKEYQTQYAAWLAKYNRADQPAPNAKSFAAPDAAAAGWKPV
                     TLPGLFAKASLPEAGAVWLRKKITVPEEAAGKNLRLELGVIRDADEVYWNGEKIGGTG
                     LAANGNPPIRRYDVPANLVKAGEATIAIRVFTQVDGAGVEIPKNGVFKAGTTDLSGEW
                     LAKVERELPPLEAAAKAEHPAEPARPMAPQLTASFLYNAMIHPLQPYAIEGAIWYQGE
                     SNAGRAYQYRKAFPMLIQDWRAQWGQGDFPFYFCQLANYRAKRDVPEESDWAELREAQ
                     TQTLALPNTGMAVL"
     gene            complement(1877..>2897)
                     /locus_tag="VIS74_08625"
     CDS             complement(1877..>2897)
                     /locus_tag="VIS74_08625"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY5793350.1"
                     /translation="AKVFSGVLSDFFRRRKALILLGYGMAALTKPLFPLAHSVETVFT
                     ARFLDRIGKGIRGAPRDALVADAAPPAIRGACFGLRQSMDTVGAVLGPAAAIFLMIGL
                     AGDIRQVLWFAVIPAFGAVLLIVVGIKEPAAPAADHRLRSPIRRDALRQFSGNYWRVV
                     AIGAVFTLARFSEAFLVLRAQEQGFSVTWLPGVILVMSLAYAVSAYPVGILSDRVDRR
                     SLLIAGLGLLILADVILARADSAFAILLGVALWGLHMGFTQGTFAAMIADVTPPELKG
                     TAFGLFNLASGVFMLFASAIAGGLWEGYGAAVTFYAGAAFSMAALILLVTWTRRRPSG
                     PANEG"
BASE COUNT          564 a         1017 c          807 g          509 t
ORIGIN      
        1 atgaggaccg ccatgcccgt gttcgggagc gccagcgttt gggtctgggc ttcccgcagt
       61 tccgcccaat cgctttcctc cggcacatcc cttttggccc ggtagttggc cagctggcag
      121 aaataaaacg ggaaatcccc ctggccccat tgggcgcgcc aatcctgaat caacatcggg
      181 aaggccttgc ggtattgata ggcccggccc gcgttgctct ccccctgata ccagatcgcc
      241 ccttcgatgg cgtaaggctg cagcggatgg atcatggcat tgtaaagaaa gctcgccgtc
      301 agctgggggg ccatcgggcg ggcgggctcc gccgggtgtt ccgccttcgc ggcggcttcc
      361 agcggcggca attcccgctc cactttcgcc agccattcgc cggacaaatc ggtcgtcccc
      421 gccttgaaaa ccccgttctt gggaatctcc acccccgcgc cgtccacctg ggtgaatacg
      481 cggatcgcga tcgttgcctc ccctgccttt accagattgg ccggcacgtc gtaacgccgg
      541 atgggcgggt ttccattcgc cgccaggccg gttccgccga ttttttcccc gttccaatac
      601 acctcgtccg cgtccctgat cacgccgagt tccaatcgga gattcttgcc ggcggcttcc
      661 tcaggcaccg tgattttttt ccgcagccac accgcgccgg cttccggcag gctggccttg
      721 gcaaacagac cgggcagcgt caccggcttc caacccgcgg cggccgcatc cggcgccgcg
      781 aagctcttcg cattcggcgc gggctggtcg gcgcgattgt atttggccag ccaggccgcg
      841 tattgggtct gatattcctt caactgatcc ggataagccg cgatcgccgc cttcaaactc
      901 tccattcttt ccttcagctc cggatccttg cccagggctt ccgcgctcgt ccacgcctcg
      961 gaaggagtgc cgccccaggc ggtgtgcagc aggccgatcg gcttattcag ctccgcgttg
     1021 agctgcttgc caaaataata ccccaccgcc gaaaacgcct cggccgtttc cggcgcggcc
     1081 acgatccatc ttcccgccac cgtatccagg ggctccggcg aggtattttt cttcacgcaa
     1141 aactgccgga accggggatt ggctgatttg gcaatctcct catcgccgtc cagcgtgttc
     1201 ttaaggaccc attccatgtt cgactgcccg gagcacaccc acacatcccc cacgagcacg
     1261 tccggaatca ccagcttgtt cttcccctcc acgatcaact cgaacggccc ctccgccttg
     1321 tctttgaggt ccagcgccac ccgccatttg ccgtccgcgc ccgcctgggt ttgcgccttt
     1381 gtgccatcca gcgccaccgt gacggcctcg cccggctcgg cctttcccca gaccggcacc
     1441 ttggcggatt tctgcagcac cgcgtgctcc gaaaaaatgg acggcaggga aacctccgcc
     1501 tgaaccgaaa acgttcccgc cagtcccagc aggaccaagc cacaaccgcg cgccagataa
     1561 ctcttcataa tggttttttt aggggtgccg ccttcagggc gaaggcggga tgagcggtta
     1621 ctactatcac ctgcccgcca ggctgcaatc gtctattttt cacgcgcccg gatcaatgcc
     1681 ggcccgtaaa aatccaatcc ccgacagccg ttatttctca aaagagattt ccgaaaaggg
     1741 atttaatgta ggggcggttg tgccaaccgc ccagcgtaaa cggggatgcg gggtccccgt
     1801 ttccccgcaa ccaaaggcgc ttggcacaag cgcctcaatc cgttggctat ttcggaaatc
     1861 tcttttgaga atcgctctaa ccttcgttcg cggggccgga aggacggcgg cgcgtccacg
     1921 tgacgagcag gatcagggcg gccatcgaaa aagccgcgcc ggcataaaag gtgacggccg
     1981 ccccatatcc ctcccacaat ccgcccgcaa tcgcgctggc gaacagcatg aaaaccccgc
     2041 tcgccagatt aaacaggcca aacgccgttc ccttcaactc cggcggcgtc acgtccgcga
     2101 tcatggccgc aaaggtgccc tgggtgaacc ccatgtgcag cccccataat gcgacaccga
     2161 gcagaatggc gaacgccgaa tccgcccggg caagaatcac gtccgccaga atcaaaagcc
     2221 ccaggccggc gatcagcagg gaacggcgat cgacgcgatc cgacaggatt cccaccggat
     2281 acgccgacac ggcataagcg aggctcatca cgaggatcac cccggggagc caggtaacgg
     2341 agaaaccctg ctcctgcgcc cgcaagacca gaaacgcctc gctgaaccgg gcgagggtaa
     2401 agaccgcccc gatggccacc acccgccagt aatttccaga aaattgccgg agcgcatccc
     2461 tccggatcgg agatcgtaag cggtgatccg ccgcgggcgc cgccggctcc ttgatcccca
     2521 cgacgatcag caaaaccgcc ccgaacgcgg gaatgacggc gaaccacaac acctgccgga
     2581 tatccccggc caatcctatc atcaaaaaaa tggcggccgc cggcccgagc accgcgccga
     2641 ccgtatccat ggactggcgc aagccaaaac acgcgccccg aatcgccggc ggggcggcgt
     2701 ccgccaccag cgcatcgcgc ggagccccgc ggatgccctt gccgatccgg tccagaaacc
     2761 gcgcggtgaa gacggtctcc accgagtgag ccagcgggaa gaggggcttg gtcagggccg
     2821 ccatcccgta gccgagcagg atgagcgcct tgcggcggcg gaaaaaatcg ctgagcacgc
     2881 cggagaaaac cttggca
//
LOCUS       DATDAY010000437         3096 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_2469864, whole genome shotgun sequence.
ACCESSION   DATDAY010000437 DATDAY010000000
VERSION     DATDAY010000437.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 3096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3096
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2469864"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..562)
                     /locus_tag="VIS74_08630"
     CDS             complement(<1..562)
                     /locus_tag="VIS74_08630"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEY5793351.1"
                     /translation="MQSIARMAIIDSQTLLQQLKWRYATKQFDPAQKIPADVWAALEE
                     SLVLSPSSFGLQPWKFLVITDPEIRAKLQPLSWGQSQIVEASHLVVFLVKHPVTSADV
                     HRYIERIAEVRGGEVAALAGFKKVMDGFVNQPAETFDSRAWASRQVYIALGNFMTSAA
                     LLGVDTCPMEGLDPAGYDKALGLEGSG"
     gene            complement(830..2701)
                     /gene="mrdA"
                     /locus_tag="VIS74_08635"
     CDS             complement(830..2701)
                     /gene="mrdA"
                     /locus_tag="VIS74_08635"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEY5793352.1"
                     /translation="MKFAGCLFLLLTAAASAQETVLDNTGVVENPKPTWETQKQARTF
                     QLDIPAPRGQITDRNGRPLAQNRLSYNLAITFPSADWTDEKALAFAIQQITLAKSLLG
                     RDIPINQEALRLHYKNRSLLPFDLIEDLTPQELNIVARGLTPNLILRQSYARIYPEKN
                     LAGHLIGYVGRVAPLSTRPIENKDLIFPDSEGREGIEQIFNDQLRGQNGQLYMTFDAD
                     GKKMSERIGRPPVPGNNIITTIDQPLQKICENVLAKNCKRGAIVIIEPSTGEILAMAS
                     WPNYDLNTFVPVLKGDAFKALQDDPDVPLLPRAFRSAYPPGSTFKTFVGLAGLQSGAI
                     KPATEFGCPTSLAVGNFVFSNWKKVDAGRLTFVKALEQSCNTWFYQAGIKTGAPAIID
                     WTTRLGLGRRTGIPLNAEEKGNIPTDEYMQRVHKRRILKGDVANMSIGQGDILITPLQ
                     MAQAMSVIAMNGQFHQIRLVKQVQALDNSVVAAYPDRLRAELPITPEIMDTLRKALVL
                     VTEGGNGTAHRAQVKGMPVAGKTGTAQWGPAGKQRTAAWFTGFLPADNPQFAFAALYE
                     GDAEDDSVHGGSHAAPLIGKVFKEYFALKKGEEKPKEDAKDGEAEKSEEEMPADESN"
     gene            complement(2718..>3096)
                     /locus_tag="VIS74_08640"
     CDS             complement(2718..>3096)
                     /locus_tag="VIS74_08640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5793353.1"
                     /translation="EALGLGFGVPVVGICSLRGYALPGAAGFAVIGDARSRQFYFARF
                     QGGDLAEGPLLLDQAGALARAGGLPLTGPAPLEAFPQLIPAFPSAAALALRAAALDPA
                     DHPPEPLYLKPAHITSCKPAKGG"
BASE COUNT          522 a          929 c         1055 g          590 t
ORIGIN      
        1 agccgctccc ttcgagtccg agcgccttat cgtagccagc tggatccagg ccctccatcg
       61 gacaagtatc cacgccgagc agcgcggcgc tggtcatgaa attccccagc gcgatgtaaa
      121 cctgccggct ggcccacgcc cggctgtcga aggtttccgc cggctggttg acgaagccgt
      181 ccataacctt cttaaatccg gccagcgccg ccacctcgcc gccgcgcact tcggcgatgc
      241 gttcgatgta gcgatggaca tccgcggagg tgaccgggtg cttgacgaga aagacgacca
      301 gatgggaagc ctccacgatc tgggactgcc cccacgacag cggctggagc ttcgcgcgga
      361 tttcgggatc ggtgatgacc aaaaacttcc acggctgcag cccgaaggag gagggggaaa
      421 gcaccaggga ttcctcgagg gcggcccaga catccgccgg aattttttgg gccggatcaa
      481 actgcttggt cgcgtagcgc catttcaatt gttgcagcag cgtttgggaa tcaatgatgg
      541 ccatgcgggc aatggactgc atgatcaccg cttcctcaag ggaaaaatcc cggccgggcg
      601 atttgggaga gcggctgcgc cgtcagtgcc ttcgaatgcg aaagtggcgc aagcttccag
      661 cttgcgaagt gttttccgat ggcaagctgg aagcttgccc cacttttcgg cttgcccttc
      721 ctattgggta taagccccgt caaatgaagc aacggacgac ggctcatcga gccgtccctg
      781 cccatataaa ggtagggcgg gatcgccgag cccgccgtgg gtaacggctc taattcgact
      841 cgtccgcggg catttcttcc tcgctctttt cggcttcgcc gtccttcgca tcctccttcg
      901 gcttctcctc gcctttcttg agcgcgaaat attccttgaa gactttgccg atgagcggag
      961 cggcgtggga gccgccgtgg acgctgtcat cctccgcatc gccttcgtaa agcgcggcga
     1021 aggcgaattg cggattgtcg gcgggcagga atcccgtgaa ccacgcggcg gtgcgctgtt
     1081 tgccggccgg cccccattgc gccgtcccgg tcttgccggc gaccggcatg cccttgacct
     1141 gcgcgcggtg agcggtgccg ttgccgcctt cggtcacgag cacgagggcc ttgcgcagcg
     1201 tgtccatgat ttcgggagtg atcggcaatt cggcgcggag ccggtcggga tacgcggcga
     1261 cgacggaatt gtccagcgcc tgcacctgct tgacgagccg gatttggtgg aactgcccgt
     1321 tcatggcgat gaccgacatg gcctgcgcca tttgcagcgg ggtgatgagg atgtcgccct
     1381 ggccgatgct catgttcgcc acgtcgccct tgaggatcct ccgcttgtgg acgcgctgca
     1441 tgtattcgtc ggtgggaatg ttgcccttct cctcggcgtt gagcgggatg ccggtgcggc
     1501 ggccgagccc gaggcgggtg gtccagtcga tgatggccgg cgcgcccgtt ttgatgccgg
     1561 cctggtagaa ccaggtgttg caggactgct cgagcgcctt gacgaaggtc aggcggccgg
     1621 cgtccacctt tttccaattg ctgaagacga aattgcccac ggccagcgag gtcgggcagc
     1681 cgaactccgt ggcgggcttg atggcgcccg attgcaagcc cgccaagccg acgaaggtct
     1741 tgaaggtcga gccgggcgga tacgcggagc ggaacgcgcg gggcagcagc ggcacgtccg
     1801 gatcgtcctg cagcgccttg aacgcgtcgc ccttgagcac gggcacaaag gtgttgagat
     1861 cgtaattcgg ccaggacgcc atggcgagga tttcgccggt cgagggctcg atgatcacga
     1921 tcgcgccgcg cttgcagttc ttcgccagca cgttctcgca gattttctgg agcggctgat
     1981 cgatggtcgt gatgatatta tttcccggaa ccggcggccg gccgatgcgt tcggacatct
     2041 ttttgccatc cgcgtcgaac gtcatataga gctgcccgtt ctggccgcgg agctggtcgt
     2101 tgaaaatctg ctcgatgccc tcgcgcccct cgctgtcggg gaaaatgagg tccttgtttt
     2161 cgatcggccg cgtggacagc ggcgccaccc ggccgacata gccgatgaga tggccggcga
     2221 ggtttttttc cgggtaaatc cgcgcgtagg actggcggag aatgaggttc ggcgtcaggc
     2281 cccgggcgac gatgttgagt tcctgcggcg tgagatcctc gatgaggtcg aacggcagca
     2341 gcgagcggtt cttgtaatgg agccggagcg cctcctggtt gatcgggatg tcgcgcccga
     2401 gcaggctttt cgccagcgtg atttgctgaa tggcgaacgc gagcgctttt tcatcggtcc
     2461 aatccgctga ggggaaggtg attgcgagat tgtagctcag gcggttctgc gcgagcggcc
     2521 ggccattgcg gtcggtgatc tggccgcgcg gcgcgggaat gtcgagctgg aacgtgcggg
     2581 cctgcttttg ggtttcccag gtgggcttgg gattttcgac cacaccggtg ttgtccagca
     2641 cggtttcctg ggcggacgcg gcggcggtga ggaggaggaa aagacaaccg gcaaacttca
     2701 tgaggtgaac gggaagatta gccgcccttt gccggcttgc aactcgtgat atgcgccggt
     2761 ttgaggtaga gcggctccgg cggatggtcc gccgggtcga gggccgccgc ccggagcgcg
     2821 agcgccgcgg cggagggaaa ggccggaatc aactgcggaa acgcctccag gggagccggg
     2881 ccggtgaggg gcaggccgcc ggcccgcgcg agggcgcccg cctgatccag cagcagcggg
     2941 ccttcggcca gatcgccgcc ctgaaaccgg gcgaaataaa actgccggct gcgggcgtcg
     3001 ccgatcaccg cgaacccggc ggcgccgggc agcgcgtagc cgcgcagcga gcaaatgccc
     3061 acgaccggaa cgccgaagcc caggccgagc gcctcg
//
LOCUS       DATDAY010000438         6241 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_1937674, whole genome shotgun sequence.
ACCESSION   DATDAY010000438 DATDAY010000000
VERSION     DATDAY010000438.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 6241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6241
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1937674"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            35..472
                     /locus_tag="VIS74_08645"
     CDS             35..472
                     /locus_tag="VIS74_08645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006984014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEY5793354.1"
                     /translation="MRIAVIADTHGRLRPELLREISGADEIWHLGDVCSEAVLSEIER
                     LGPPVWVVRGNNDFVPRWPLQRKLERGGRTFRLVHIPPPHPGGADFLLHGHTHVPRDE
                     QVGATRVLNPGSAGLANKGAPLSFAWLAVDPATGGVGWRIQPL"
     gene            582..672
                     /locus_tag="VIS74_08650"
     tRNA            582..672
                     /locus_tag="VIS74_08650"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:616..618,aa:Ser,seq:gct)
     gene            830..3088
                     /locus_tag="VIS74_08655"
     CDS             830..3088
                     /locus_tag="VIS74_08655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEY5793355.1"
                     /translation="MENIPPAFHLAFVGDYVPRRCGIATFTADLCEAIAAEFPKTKCI
                     VGSVNDREEGYDYPSRVRFEIEEKEIASYHRAAEFLNINNVEVVSVQHEFGIYGGPAG
                     SHLLAMLRDVRMPVVTTLHTVLANPNADQRAVMQELDARSNRFVVMADRGRHLLETVY
                     GVAPEKIDLIPHGVVDLPFMDSNFYKDEFGVEGKTVLLTFGLLSPNKGIEHVIEAMPA
                     ILERHPEVVYLVLGATHPNLLAVEGEKYRRKLENLAKSLGVSKQVIFYNRFATLEELK
                     EFIGAADIYLTPYLNVAQITSGTLAYAFGAGKAIISTPYWHAEELLAEERGILVPFSD
                     AAAIATGVNDILSQPTRMTAMRKRAWKQGRDMIWPVVARRYMESFGRARAGMSEVSVA
                     APSVRPQPMPLPELKLDHLFTMSDHSGIFQHAIYNVPNFHEGYCTDDNARAFIFTVLL
                     EELRGPSKEMERLASGCLAFLWYAFDGNTCRFRNFMSYSRQWLELQGSEDSHARALWA
                     VATALGRSKNPGHRNLCALLFQRGLPPVARFSSPRAWAFTLIAIHEYLRTLSGDRAVS
                     QMRDTLTAMLVALFHANSSPDWQWFERIATYDNAKLSHAMILSGHWTSQGGTLEIGLA
                     SLRWLLEAQTAPDGHFAPIGCHGFWPRGGEKARFDQQPLEAHAMISACLEAFFVTRDE
                     FWRGAARRCFDWFLGRNDLGQPLYDASTGGCRDALLQDHLNQNQGAESSLAFYLSWVE
                     LKRAEESEQAAL"
     gene            3085..4548
                     /locus_tag="VIS74_08660"
     CDS             3085..4548
                     /locus_tag="VIS74_08660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 130 protein"
                     /protein_id="HEY5793356.1"
                     /translation="MSEVNVQSTGIFFRPDNRRVLVRPFIPSDPVRVAHILARALALT
                     EAEVEAQLMILRNDFNARHLDLETYWRRHFEMVRGRLANQSLSFNRQLYLGALFSGEY
                     ALESAALFNPSVVAHPDQGGLGEGALRFVLSLRATGEGHISSIEFRSGILHADDSIEI
                     EETSLWVVAPGVKENPTYRKTIFLHKLAEMGFENEGSRRIMNPLGEDFTHEELNKAMR
                     SVAGQGKRPSREWARTMECVQWLARSNYEVHFQSSVPLSQRVIFPVSPNESNGIEDAR
                     FVRFVEDDGSVCYYATYTAYNGRAILPQLIETPDFLSFRVRTLNGQAVQNKGMALFPR
                     RINGQFVMISRQDDENLFLMFSGNLHFWNEPRPLRIPRHPWEFVKIGNCGSPIETGAG
                     WLVLTHGVGAMRRYCIGAMLLDLEDPSRVLGALEKPLLEPIGEKREGYVPNVVYSCGA
                     MVHRGRLILPYALSDTSSTIATIELAELLKALLANPV"
     gene            complement(4685..5008)
                     /locus_tag="VIS74_08665"
     CDS             complement(4685..5008)
                     /locus_tag="VIS74_08665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5793357.1"
                     /translation="MSPYSAFSRRGPDIVTEELVTVLSGAKSYEFKALYEVIQSALGR
                     RNVGGGDEILRLRTYDKLQNLVLHGQVKKTGKQYKGLKKPLSLLAANLKSLRENGPSF
                     RRLTE"
     gene            complement(5214..6137)
                     /locus_tag="VIS74_08670"
     CDS             complement(5214..6137)
                     /locus_tag="VIS74_08670"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HEY5793358.1"
                     /translation="MNTETSAYHLPLDLAVRVGCKISYEAAAPTPVLAICKPRQDAYQ
                     SIRQESMQFDPDLSATEFEDDHGNIVYRLTLKPGPNTLRYDAIVMVPSQPEDYHWLDE
                     PIPPHLLPAPILRYTLPTRYCDSDKLLNFAWEHFGHLEQGLARVKGICQWLHDNIEYR
                     TLSGSPNLSAHDIIERRYGVCRDFAHTTVALCRTFNLPARYVSGYLPDIGFQDAGTPQ
                     DFHAYCEVYLGGRWQIFDARYNQPRIGRIRIAAGYDAVNCAFTTLYGQAALTRFEVWG
                     YQMDPAVVHIDDPLDLSKRLCGTPRVIHPPR"
BASE COUNT         1186 a         1786 c         1906 g         1363 t
ORIGIN      
        1 aacttaagac ttacaaactt aaaactcgcc tcccatgcgc attgccgtca tcgccgacac
       61 ccacggccgc cttcgtcccg aattgctgcg ggaaatttcc ggggccgatg agatctggca
      121 tctcggcgac gtgtgctcgg aggccgtgtt gtccgaaatc gagcgattgg gaccgccggt
      181 ctgggtggtc cggggcaata atgactttgt gccccgctgg ccgctacagc ggaagctgga
      241 gcggggcggg cggaccttcc ggctggttca catcccgccg cctcatcccg gcggggcgga
      301 tttcctgctc cacggccaca cgcacgtgcc gcgcgatgaa caggttggcg ccacccgcgt
      361 gctcaatccg ggctcggcgg gactggccaa caagggcgcg ccgctgagtt ttgcctggct
      421 ggccgtggac ccggcaaccg gcggggtggg ctggcggatt cagccgcttt aacggaggca
      481 tggttaaatt ttcttgcccg tttggaaaaa ccacgccaaa tttcggaaca tttttttgcg
      541 ggttgcattc ccgtcccggt tgttcttgaa ctgatcccat tggtgaggtg gctgagtggt
      601 cgaaagcaaa gctttgctaa agcttcgtac tccaaaaggg taccgagggt tcgaatccct
      661 ccctcaccgc catttttgat cgagtggcgt gacctctccg gggcgccgcg gttgactttc
      721 cgttaaggca ggcgataatt aggaagtttc gtgcaggatt cgcttgccct tcgggcgatc
      781 aggattgctg ccacccttga ggcgcgcgat tccgcacatt cgccttttca tggaaaatat
      841 tccccccgca ttccacctcg cctttgtcgg ggattatgtt ccgcgccgtt gcgggatcgc
      901 caccttcacg gccgacctct gcgaggcgat cgcggcggag tttccgaaga ccaaatgcat
      961 cgtcggctcc gtgaatgacc gggaggaagg gtatgactat cccagccggg ttcgatttga
     1021 gatcgaggag aaggaaatcg cctcctatca ccgcgcggcg gaatttttga atattaataa
     1081 cgtcgaagtc gtctcggtgc agcacgagtt tggcatctac ggcggtccgg ccggcagcca
     1141 tctgctggcg atgttgcggg acgtgcgaat gcccgtggtc acgacgcttc ataccgtgct
     1201 ggcgaacccc aatgcggatc agcgcgccgt catgcaggaa ctggacgcgc gttcgaatcg
     1261 tttcgttgtc atggcggatc gcggccggca tttgctggaa accgtctatg gggtggcgcc
     1321 ggaaaagatt gatctcattc cgcacggcgt ggtggatctg cctttcatgg actcgaactt
     1381 ctacaaggac gagtttggcg tggaaggaaa gacggtgctg cttactttcg ggctgttgtc
     1441 tccgaacaag ggcattgagc atgtgatcga ggccatgccg gcgattctgg aacggcatcc
     1501 ggaggtggtt tacctcgtgc tcggcgccac tcacccgaac ctgctggcgg tcgagggaga
     1561 aaaataccgg cgcaagctgg agaacctcgc aaaaagcctc ggggtttcca aacaggtgat
     1621 tttttacaat cgttttgcga ccctggagga attgaaggaa ttcatcggcg cggcggacat
     1681 ttacctcacg ccgtatctga acgtggcgca gatcacctcg ggcacgctgg cctacgcgtt
     1741 tggcgcggga aaggcgatca tctcgacgcc ttactggcac gccgaggaac tgctggcgga
     1801 ggagcgcggc atcctggtgc cgttttcgga tgcggcggcc atcgccacgg gggtaaacga
     1861 cattctttcc cagcccaccc gcatgaccgc gatgcggaag cgggcctgga agcagggccg
     1921 ggacatgatc tggcccgtcg tggcccgccg ttacatggaa agtttcggcc gggcgcgggc
     1981 cggcatgagc gaggtttcgg tggccgcccc aagcgtccgg ccccagccaa tgcccctgcc
     2041 cgaactgaag ctcgatcacc ttttcacgat gagcgaccac tccgggattt ttcagcacgc
     2101 gatttataat gtgccgaatt ttcacgaggg ctactgcacc gatgacaacg cccgcgcctt
     2161 tatttttacg gttctgttgg aggaattgcg cggtccgtcg aaggagatgg agcgtctcgc
     2221 cagcgggtgt ctcgcattcc tttggtatgc cttcgacggc aatacctgcc gcttccggaa
     2281 tttcatgagc tattcccgcc agtggctgga actacagggg tcggaggaca gccacgcgcg
     2341 ggcattgtgg gcggtggcca ccgcgctcgg acgttcgaaa aatcccggtc accgcaatct
     2401 ctgcgcgttg cttttccagc gcggtttgcc gccggtggcg cgcttcagct cgccgcgcgc
     2461 gtgggcgttc acgctcatcg ccattcacga atacctgcgc accctttcgg gggaccgcgc
     2521 ggtgagccag atgcgcgata cgctcacggc gatgctcgtg gcgttgtttc acgccaactc
     2581 ctcgcccgat tggcaatggt tcgaacgcat cgcgacctac gataatgcca aattatcgca
     2641 cgcgatgatc ctgagcggtc attggacttc gcagggcggc acgctggaaa tcggactcgc
     2701 ctccctccgc tggctgctgg aagcgcagac tgctccggac ggacattttg cgccgatcgg
     2761 gtgccacggc ttctggccgc gcggagggga gaaggcgcgg tttgaccagc aaccgctgga
     2821 agcgcatgcg atgatctccg cctgcctgga ggcatttttt gtgacccggg atgagttttg
     2881 gcgcggagcc gcccgccggt gcttcgattg gttcctcgga cggaacgatc tcggccagcc
     2941 tttgtatgac gcctccaccg gaggatgccg cgacgcattg ttgcaggatc acctcaatca
     3001 aaaccagggg gcggaatcca gcctcgcttt ttatctctcg tgggtggagc tgaaacgagc
     3061 cgaggaaagc gaacaggcgg ctttatgagc gaagtcaacg tccagtccac gggcatcttt
     3121 tttcggcccg acaaccggcg ggttttggtc cgtcctttca ttccctcgga tcccgtgcgg
     3181 gtggcgcaca tccttgcccg cgccctggct ttgaccgagg cggaggtgga ggcccaattg
     3241 atgattcttc ggaatgactt caacgcccgc cacctcgatt tggaaaccta ttggcggcgg
     3301 catttcgaaa tggttcgagg ccgcctggcg aatcaatccc tttcctttaa tcgtcaactt
     3361 tacctcggcg cgcttttctc gggcgaatac gcgctcgaat ccgccgcgct atttaatccc
     3421 tccgtggttg cccatccgga ccagggcggg ctgggggaag gcgcgcttcg tttcgtcctg
     3481 agtttgcggg ccaccggcga ggggcacatt tcctcgatcg agtttcgctc gggaattctc
     3541 cacgcggatg attcgattga aattgaggaa acctccttgt gggtggtcgc tcccggggtg
     3601 aaggaaaatc ccacctatcg caagaccatc ttcctgcaca agctggcgga gatgggattc
     3661 gaaaacgaag ggtcgaggcg aatcatgaac ccgctcgggg aggacttcac tcacgaggaa
     3721 ttaaacaagg ccatgcgatc cgtcgccggc caggggaaac gcccgtcccg cgaatgggct
     3781 cgcacgatgg aatgtgtgca gtggctggcc cgttcaaact acgaggtgca ctttcaatcg
     3841 tcggttccct tgtcccagcg cgttattttc ccggtatccc ccaacgagag caacgggatc
     3901 gaagacgcgc gcttcgtccg atttgtcgaa gacgacggct ccgtttgcta ctacgcgact
     3961 tacaccgcgt ataacggccg cgccatcctg cctcagttga tcgaaacgcc ggattttctc
     4021 agcttccgcg ttcgcaccct gaacggccaa gccgtgcaga acaagggcat ggcgctcttt
     4081 ccccgccgca tcaacggtca gttcgtcatg atttcccgcc aggatgacga gaatcttttc
     4141 ctgatgtttt ccggcaattt gcatttttgg aacgagcccc ggccgcttcg gattccccgg
     4201 catccctggg aattcgtgaa aatcggcaat tgcggatcgc cgattgaaac cggcgccggc
     4261 tggctcgtcc tgacgcatgg agtgggagcg atgcgccgct attgcatcgg cgcgatgttg
     4321 ctggatctgg aagatccgag ccgcgtgctc ggcgctttgg aaaagccgct gctggaacca
     4381 atcggcgaaa agcgcgaggg ttatgtgccg aacgtcgttt attcctgcgg cgccatggtt
     4441 catcggggac ggctgattct gccctatgcc ctcagcgaca cttcctccac gattgccacc
     4501 attgagcttg ccgagcttct gaaggcgctt ctcgcgaatc cggtttgact ttcgccggcc
     4561 gggccggcga aagctcaagc ggtgaaattt gaatgattgc gaatgctccg cgcgcggcag
     4621 atcctcagaa cgaagccgct tcatccgcgc ggtggttttg cggctgattt tctggctggc
     4681 tgaattattc cgtcaaacgg cggaatgagg ggccattctc ccgaagtgac ttgagattgg
     4741 ccgccaggag ggagagcggt ttcttcagcc ccttatactg cttgccggtt ttttttacct
     4801 gcccgtgcag gacgaggttt tgcagcttgt cataagtccg cagccgcagg atttcatcgc
     4861 cgccgcccac attgcggcgg ccgagggcgg attgaatcac ctcgtagaga gccttgaatt
     4921 cataagactt cgcgccggat aaaacggtga ccagctcttc ggtgacaatg tcgggaccgc
     4981 ggcgggaaaa agcggagtat gggctcatga gtgggttcgg gataattttc ggcggatgct
     5041 tccgcatcac ggattttttt aaatcgcctc gacaacctgc ggtgggtccg ctccgagcaa
     5101 aactggcaat tcaccaacgt tgaaagctct attttttcat taaagcccgc tttgggaata
     5161 tcgcaagcat atcccgcgat tttgcgtttc atccccccat gggacgatga tctttagcgc
     5221 ggagggtgaa tgacccgcgg ggttccgcac aatcgtttgg aaagatccag cgggtcgtca
     5281 atatgcacca ccgccggatc catctggtat ccccaaacct cgaatcgcgt cagcgccgcc
     5341 tgcccataga gcgtggtgaa ggcgcaatta accgcatcgt atcccgcggc gatccggatg
     5401 cggccgatcc gcggctgatt ataacgcgcg tcgaaaatct gccagcggcc tccgagatac
     5461 acttcgcagt aggcatggaa atcctgggga gtgccggcat cctgaaagcc aatgtcgggc
     5521 aggtagccgc tgacataacg cgccggtaaa ttgaaggtcc ggcaaagggc gacggtggta
     5581 tgcgcgaagt cccggcaaac gccgtaacgc cgttcgatga tgtcatgcgc ggagagattc
     5641 ggactgccgg agagcgtgcg gtattcgatg ttgtcgtgca gccattggca gatgcccttc
     5701 acccgggcca ggccctgctc caggtgcccg aaatgttccc aggcgaaatt cagcaatttg
     5761 tcggaatcgc agtagcgcgt cggcagcgtg tagcgaagga tcggggcggg cagcaggtgg
     5821 ggcggaatgg gttcatccag ccagtgataa tcctccggct ggctcggcac catgacgatg
     5881 gcgtcgtaac gcagcgtgtt cgggccgggt ttcagcgtca gccggtaaac gatgtttcca
     5941 tgatcatcct cgaattcggt ggccgaaagg tcggggtcga actgcatgct ttcctggcgg
     6001 atggattgat aggcgtcctg ccggggtttg cagatggcga gaaccggcgt gggggcagcg
     6061 gcttcatagg aaattttaca gcccaccctc acggccagat ccagaggcag atggtaagct
     6121 gaagtttctg tgttcaagtc gtcttgggca tgcgaggttg aacgcatggc ttgcctatca
     6181 tcggatcata agggcaaggt gacaaatgga attcttcgat ttcacagcct ctgagagatg
     6241 a
//
LOCUS       DATDAY010000439         2488 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_703505, whole genome shotgun sequence.
ACCESSION   DATDAY010000439 DATDAY010000000
VERSION     DATDAY010000439.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2488)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2488)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2488
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_703505"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..338
                     /locus_tag="VIS74_08675"
     CDS             <1..338
                     /locus_tag="VIS74_08675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5793359.1"
                     /translation="NLPARNPLVSRVDINPAGLIGVGSTIRIEGSGVSVRLIVQEFAG
                     PGRIAFTVKAGKTFGGSLVVLTPSETGTLVDHTLSLELRGAGKLFSLLIGPAVKKEAA
                     ALRKWVETH"
     gene            570..>2488
                     /locus_tag="VIS74_08680"
     CDS             570..>2488
                     /locus_tag="VIS74_08680"
                     /inference="COORDINATES: protein motif:HMM:NF021424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HEY5793360.1"
                     /translation="MSIHVALKHRTTYLYDRPVSMGPQVIRLRPAPHCRTPILSYSIK
                     IHPEEKFVNWQQDPFSNYLARVMVLEKTEKFEVEVEVVAQMAVYNPFDFFLEPAAEKF
                     PFTYDPALAHDLAPYLKCEASGPLFERYFKAIPKKEQRTIDFLVGLNQLVQGDIGYLV
                     RMEPGVQEPEETLEKRSGSCRDSAWLLCNLLRRCGLAARFVSGYLIQLKSDVKSLDGP
                     SGTEVDFTDLHAWTEVFLPGAGWIGFDATSGLLAGEGHLPLACTPEPSTAAPISGTTS
                     EGKAEFEFEMSVTRIYESPRVTKPYTEEQWAAILALGDRVDEHLKKGDVRLTMGGEPT
                     FVAIDDVDGAEWNAEAMGPMKRGLADSLLRKLQARFAPGALLHHGQGKWYPGEQLPRW
                     AFGCFWRKDGVPIWENQKWIADEAVNYGVNDVDSMRFLEALAHRLDIDPRFIQPAYED
                     AWYYLWKERRLPVNVDPLKSNLKDELERERIARVFEQGLNKVIAHVLPIRYRDGWMGG
                     AWFLRPETLYLVPGDSPAGFRLPIDSIPWVKESEYPWIYEPDLIAERPPLPLRPERKT
                     QAFREAGQLTEEYIRYLRRGDERGFRPQGAPGSGFEIPEGGGELGSEGRRGPGGRPGG
                     RPPWTVPQKGESAPW"
BASE COUNT          445 a          710 c          831 g          502 t
ORIGIN      
        1 ccaatctccc ggctcgcaat cccctcgtca gccgcgtgga cattaatcct gccggactca
       61 tcggggtcgg ttcgaccatc cggatcgagg gttcgggtgt ttccgtccgc ctcatcgtgc
      121 aggaatttgc cggccccgga cgcatcgcct tcaccgtcaa ggcgggcaag acgttcggcg
      181 gttccctggt cgtcctcacg ccttcggaaa cgggcacgct tgtcgatcac acgctcagcc
      241 tcgaactccg gggcgcgggc aagctgtttt ccttgctgat cggccccgcc gtgaaaaaag
      301 aggcggccgc tctccggaag tgggtcgaaa cgcattgagc cccgccggga gacattccgg
      361 ttgatccggc atccctgcct ccgcgttttg gagcgatggc ggcagtggaa tttgcccgga
      421 gagaagaaaa tgggaaggat tcctcccggc cgtgaaagtc cggcaaccga ttcgttaaaa
      481 atgacttgcg ggaaactttc ggaagagttt gcggctcatt ttcactttga gctcgccttt
      541 tacccgatgg cattttagct gaggcggtta tgtccatcca cgtcgccctt aaacatcgta
      601 ccacctattt gtatgatcgg cccgtttcga tgggtccgca ggtcatccgc ctccggccag
      661 ccccgcattg ccgcacgccg atcctgagtt actcgatcaa aatccatccg gaggaaaagt
      721 tcgtgaactg gcagcaggat ccgttcagca attatctggc gcgggtcatg gtgctggaga
      781 agacggagaa gtttgaggtg gaggtcgagg tggttgccca gatggcggtg tataatccat
      841 tcgacttctt tctggaaccg gcggcggaaa aatttccgtt cacctacgat ccggcgctgg
      901 cgcatgattt ggcgccgtat ctgaagtgcg aggcgtccgg cccgctgttt gagcggtatt
      961 tcaaggcgat tccgaagaaa gagcagcgaa cgattgattt tctggtggga ctcaaccagc
     1021 tcgtgcaggg tgacatcggc taccttgtcc gcatggaacc gggggtgcag gaaccggaag
     1081 agaccctgga aaaacgttcc ggctcgtgcc gtgactcggc gtggctgctt tgcaatttgc
     1141 tgcgccgctg cggtctcgcg gcgcggtttg tttccggcta cctcattcag ctgaagtcgg
     1201 atgtgaagtc gctcgacggc ccctccggca cggaggtgga tttcaccgat ttgcatgcct
     1261 ggacggaggt gtttctgccg ggcgcgggct ggatcggctt cgacgccacg tcgggcctgc
     1321 tcgcgggcga agggcacctg ccgctggcct gcacgccgga accttcgacg gcggcgccga
     1381 tctcggggac gacgtcggag ggcaaggcgg agtttgaatt tgagatgtcc gtgacgcgca
     1441 tctatgagtc gccgcgggtc accaaacctt acacggagga gcaatgggcc gccattctcg
     1501 cgctgggcga ccgggtggat gagcatctga aaaaaggcga tgtccggctg acgatgggag
     1561 gcgagccgac ctttgtggcg attgatgacg tggatggcgc cgagtggaac gccgaggcga
     1621 tgggtccgat gaagcgcggg ctggcggatt cgctgttgcg caaactccag gcgcgtttcg
     1681 cgccgggcgc gctgctgcac cacggccagg gcaaatggta tccgggcgaa caactgccgc
     1741 gctgggcgtt cggttgtttc tggcgcaagg acggggtgcc gatttgggaa aatcaaaagt
     1801 ggatcgccga tgaggcggtg aactacggcg tgaacgatgt ggattcgatg cgttttctcg
     1861 aagcgctcgc gcaccggctt gacattgatc cgcggttcat ccagccggcc tatgaggatg
     1921 cctggtatta cctgtggaag gagcgccggc tgccggtgaa cgtcgatcca ttaaagtcga
     1981 acttgaagga tgagctggaa cgcgagcgca tcgcccgcgt ttttgaacag ggcctgaaca
     2041 aggtgatcgc ccacgtgctg ccgatccgtt accgcgatgg ctggatgggc ggagcctggt
     2101 tcttgcggcc ggagaccctg tatttggtcc ccggcgactc gcccgccgga ttccggctgc
     2161 cgattgattc gatcccctgg gtgaaagagt ccgagtatcc ctggatctac gagcccgatc
     2221 tgatcgcgga acgtccgccc ttgccgctgc gccctgagcg gaaaacccag gcgttccggg
     2281 aagccgggca gcttaccgag gaatacatcc gctatttgcg gcgaggcgac gagcgcgggt
     2341 tccgtccaca gggagccccg ggcagcgggt tcgagattcc ggagggcggg ggggagctcg
     2401 ggtccgaggg gcgccgcgga cccggcggcc ggccgggcgg ccggcctccg tggaccgttc
     2461 cacaaaaggg tgaatccgcg ccgtggat
//
LOCUS       DATDAY010000440         4764 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_105489, whole genome shotgun sequence.
ACCESSION   DATDAY010000440 DATDAY010000000
VERSION     DATDAY010000440.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 4764)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4764)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4764
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_105489"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1217
                     /gene="atzF"
                     /locus_tag="VIS74_08685"
     CDS             <1..1217
                     /gene="atzF"
                     /locus_tag="VIS74_08685"
                     /EC_number="3.5.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF006043.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004039 - allophanate hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="allophanate hydrolase"
                     /protein_id="HEY5793361.1"
                     /translation="SGRVPAAFNELIGFKPTVGRWSTSGLLPACRTLDCITVFTRQVD
                     EAARVDEIVRGFDAADSFSRKPHPPLAGGNRIGILAENQREFFGDLEYARLYAEAIAK
                     ARALGWEVAEFDYEPFRQAANLLYSGPWVAERYAAIREFIEGRPGAIHPAVRTIIEGA
                     RKFTALDAFQSRYQLAGLARQAEKTWESFDAILLPTAGTIYTIDQMRADPIRLNTHLG
                     HYTNFVNLLDLCAIAFPAGRRADGLPFGVTLIGPAWSDESQAALAAQFAGQAFEVKTQ
                     PIQLAVVGAHLRGLPLNFQLRELGARFVRAARTAPAYRLYALPNTTPPKPGLARTGEG
                     GTAIELEIWELSPESFGRFVNAVPPPLAIGNVQLESGKWVKGFVCEPLALQEARDISE
                     FGGWRAFLAAGK"
     gene            complement(1404..2717)
                     /locus_tag="VIS74_08690"
     CDS             complement(1404..2717)
                     /locus_tag="VIS74_08690"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF005301.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HEY5793362.1"
                     /translation="MSELKAADLVIRSLGERTIHSPLEKFFAQLGTNHAFYSDDHRIA
                     VDHRLGKLKNAPEIPCFEASGPRRNIYFKPSEVKCGIVTCGGLCPGLNDVIRTIFIQA
                     HYRYGVHEIYGIPYGYEGLIQDYGHRIIQLTPEYISNLHRFGGTALGTSRGPQDVGRM
                     VDRLQELGINILFVIGGDGSQRGALAIEKEAHSRGLKLSVVGVPKTIDNDLIFMDKSF
                     GYETACAAAVEAINAAHTEARSSRNGIGMVKLMGRHSGFIASSAAIASGEVNCVLIPE
                     VPFVLDGDNGLLEYLRQRVVHRHHAVVVVAEGAGQELFSNHEATDASGNPKLHEVGGF
                     LKGKIEAYFKQTKTDLTLKYIDPSYIIRSVPASPQDRIYCMRLAQAAVHAAMAGKTGL
                     VVARWHGAYVHVPISVVTSARRMVDSGGDLWLSVLESTGQPARFY"
     gene            complement(2833..3708)
                     /locus_tag="VIS74_08695"
     CDS             complement(2833..3708)
                     /locus_tag="VIS74_08695"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HEY5793363.1"
                     /translation="MSRLRILHTTTYNYAEPVEFGPHRLVLRPREGHDLQVESLDLRI
                     EPPATVTWQRDVFGNSIAFAHFSDPSERLEFQNEVTIFRRDPDSSAAPAGSLAARLPL
                     EYSPFEEPVVRGYLAPVYPEESALLQQWAGGQFTAGDGDEAMSLLGEIGEWIRQHIQY
                     RRREDRGVQSPLETLRLRSGSCRDMATLLLETLRSLRLPARFASGYLDSAASAAGRAA
                     THAWTEVYFPQYGWRGYDPTLGEPTSHKHIVTGVSSHPRGVMPVSGSYHGPSGITLGM
                     AVSVKIDRLSAIVLS"
     gene            complement(3705..4598)
                     /locus_tag="VIS74_08700"
     CDS             complement(3705..4598)
                     /locus_tag="VIS74_08700"
                     /inference="COORDINATES: protein motif:HMM:NF044442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HEY5793364.1"
                     /translation="MESAPPPQSGSPTRFHLSCHLEYNVAAPVALLCNFRARTTPFQT
                     IQWEKVSIQPEMPAELVVTEPDQNRFDRLKISRNQTLALDYEAVVEASHQFLNPEQLR
                     KVSPAHLDPAQLPFLLPSRYCQSDLLGRFAWQKFGQMTDSYDQVQAITQWIHENIEYV
                     PGTTTSATSAYDTVTQCAGVCRDFAHLGIALCRALNTPARYFTGYAFQLNPPDFHACF
                     EVFIGGSWLLFDATRLVPLNGLVSIGAGRDAADVSVCTVFGNARLLTQSVTSKVESPE
                     FVPLQEAQFNEVAISLDARKS"
BASE COUNT          916 a         1336 c         1581 g          931 t
ORIGIN      
        1 gatccggccg cgtgccggcg gcgttcaatg aactcatcgg tttcaagccc acggtgggcc
       61 gctggtccac gtcgggcctg ctgcccgcat gccggacgct ggattgcatc acggttttca
      121 cccggcaggt ggacgaggcg gcgcgggtgg atgagatagt gcggggattc gacgcggcgg
      181 attccttttc ccgcaagccg cacccgccgc ttgcgggcgg caaccgcatc ggcatcctgg
      241 ccgaaaatca gcgggaattt tttggcgatc tcgaatatgc ccggctctac gcggaggcca
      301 tcgccaaggc gcgggctttg ggctgggagg tggcggagtt tgattacgag cccttccgcc
      361 aggcggcgaa tttgctctac agcggcccgt gggtcgcgga gcgttacgcg gcgatccgcg
      421 agttcatcga aggccggcct ggcgcgattc accccgcggt gcgaacgatc atcgaaggcg
      481 cgaggaaatt caccgcgctg gatgccttcc aatcccggta tcaattggcc ggcctcgccc
      541 gccaggcgga aaaaacgtgg gaatccttcg acgcgatcct gctcccgacg gcggggacca
      601 tttacaccat cgaccaaatg cgggcggatc ccatccggct gaacacccac ctggggcatt
      661 acacgaattt cgtgaacctg ctggatcttt gcgccatcgc gtttcccgcg ggccgccgcg
      721 ccgatgggct gccgtttggc gtgacgctga tcggtccggc gtggtcggat gaatcccagg
      781 ccgctctcgc ggcgcagttt gccgggcagg ctttcgaggt caaaacccag ccgatccagc
      841 ttgccgtcgt gggtgcgcac ctgcgcgggc tgccgctgaa ttttcaattg cgggaattag
      901 gcgcgcggtt tgtgcgggcg gcccggacgg ctccggctta ccggctttac gcattgccga
      961 ataccacgcc gcccaaaccg ggattagcgc gaaccgggga ggggggaacc gcgatcgaat
     1021 tggaaatttg ggagctctcg ccggaaagtt tcggccggtt tgtgaacgcg gtgccgccgc
     1081 cgctggcgat cggaaacgtc cagctcgaat ccgggaaatg ggtgaaggga tttgtctgcg
     1141 aacccctggc gcttcaggag gcgcgggata tcagcgaatt tggcggctgg cgggcttttc
     1201 ttgccgcagg caaataaacc gcctccgccc cttccaggcg gaagaaaaag ggaaggcaaa
     1261 atacttgtta atgcgcttga atattcccaa gcaattgatt gaaaggatga taatctgatt
     1321 ttccaaagct atttcacaag ttattcaccg gcttgaaatg acgcggcgcg gcgccgtttt
     1381 ccctattttg ggggtggcgg agcctagtaa aaccgcgcgg gctggccggt ggattcgagc
     1441 accgagagcc agagatcccc gccggaatcg accatgcggc gggcggaggt gaccacgctg
     1501 atgggcacat ggacgtaggc gccgtgccag cgggcgacga cgaggccggt tttgcccgcc
     1561 atggcggcgt gaacggcggc ctgggccagg cgcatgcaat aaatgcggtc ctgcggcgag
     1621 gcgggcacac tgcggatgat gtagctggga tcgatgtatt tcagcgtgag atccgtcttc
     1681 gtttgtttga aatacgcttc gatcttgcct ttgaggaagc ccccgacttc gtgcagtttc
     1741 ggatttccgg aggcgtccgt ggcttcatgg ttgctgaaaa gttcctgtcc cgcgccttcg
     1801 gcgacgacca ccacggcgtg atgccgatga acgacgcgct ggcgcagata ttccaagagc
     1861 ccgttgtcgc catcgagcac gaacggcact tccggaatca acacgcagtt gacttcgccg
     1921 ctggcaatgg cggcggaact cgcgatgaag ccggagtgcc ggcccatgag cttgaccatg
     1981 ccgatgccat tgcgggagct gcgggcctcg gtgtgcgcgg cattgatggc ttccaccgcg
     2041 gcggcgcagg cggtttcata accgaagctc ttgtccatga agatcaggtc attatcaatg
     2101 gtcttcggca cgccgacgac cgagagcttc aacccgcggc tgtgcgcttc cttctcgatc
     2161 gccagggcgc cgcgctggct gccgtcgccg ccgatcacga aaagaatatt gatcccgagt
     2221 tcctggaggc ggtccaccat ccggcccacg tcctgcggcc cgcgcgaggt gccgagggcg
     2281 gtgccgccaa aacggtggag gttgctgatg tattccgggg tgagctggat gatgcggtgc
     2341 ccgtagtcct gaatcagccc ctcgtagccg taggggatgc cgtaaatttc gtgcacgccg
     2401 tagcgataat gcgcctggat gaaaatggtg cggatgacgt cattcagccc cgggcacagc
     2461 ccgccgcagg tgacgatgcc gcatttgacc tcggaaggct tgaaataaat gttccggcgg
     2521 ggaccggacg cttcaaagca gggaatttcc ggcgcatttt taagtttgcc cagccggtga
     2581 tccacggcaa tgcggtgatc atcgctgtaa aacgcatggt tggtgcccaa ctgggcgaag
     2641 aatttttcga ggggtgaatg aatcgtccgc tcgccgaggg agcgaattac aaggtcggct
     2701 gctttcagtt cgctcataga aggtggttcc ctaaatccgt gttgttgtgt gaagcaaaac
     2761 gccggttttg caaagtgaaa tgtcggcaac gttttgcggg catccgaaac cgccagcagg
     2821 aatcgttccg gtttacgaaa gaacgatggc ggaaagccgg tcgattttga cggataccgc
     2881 catgcccagg gtaatgccgc tcgggccgtg gtaggaaccg ctgacgggca tgacgccgcg
     2941 cggatgcgag ctgacgccgg tcacgatgtg cttgtgcgag gtgggttcgc ccagggtggg
     3001 atcgtagcct ctccatccat attggggaaa ataaacctcc gtccacgcgt gggtggcggc
     3061 ccggccggcg gcggaagccg cgctgtcgag gtaaccgctg gcgaagcgcg ccggcagccg
     3121 gagggagcgc agcgtttcca acagcaaggt cgccatgtcc cggcaggaac cggaacgcag
     3181 ccggagggtt tccagggggg attgcacgcc ccggtcttcc cggcggcggt attggatgtg
     3241 ctgccggatc cattcgccga tttcgcccag caagctcatg gcctcgtcgc catcgccggc
     3301 ggtgaattgc ccgcccgccc attgctgcag cagggcggat tcctcgggat agacgggggc
     3361 gagatacccg cgcacgacgg gttcctcaaa gggggaatat tccaagggca atcgggcggc
     3421 caaactgccc gccggcgcgg cggacgaatc cgggtcgcgg cgaaaaatcg tcacctcatt
     3481 ctggaattcg agccgctcgg aagggtccga aaaatgcgcg aacgcgatcg agttgccgaa
     3541 tacgtcccgc tgccaggtga cggtggcggg gggctcgatc cgcagatcga ggctttccac
     3601 ctggagatca tgcccttccc gtggccgcaa caccaagcgg tgcggcccga attccaccgg
     3661 ttcggcgtaa ttatacgtgg tggtgtggag gatgcggagg cggctcatga tttccgggcg
     3721 tccaaggaaa tcgccacctc gttgaactgc gcttcctgga gcggaacgaa ttccggcgat
     3781 tcgactttgc tggtgaccga ctgggtgagg aggcgggcat tgccaaagac ggtgcagacg
     3841 gaaacgtccg cggcatcccg gcccgcgccg atggagacca ggccattcag gggaacgagc
     3901 cgggtcgcgt cgaaaagcag ccagctgccg ccaatgaaaa cctcgaagca ggcgtggaaa
     3961 tccggcggat ttaattgaaa ggcgtaaccc gtgaagtaac gcgccggcgt gttgagcgcg
     4021 cggcaaaggg cgattccgag atgcgcgaaa tcccggcaga cgcccgcgca ttgggtcacc
     4081 gtatcgtaag ccgacgtggc cgaggtcgtg gttccgggca cgtattcgat gttttcgtga
     4141 atccattggg tgattgcctg gacctgatcg taggaatcgg tcatctggcc gaatttttgc
     4201 caggcgaagc gcccgagcaa atccgactgg caatagcggc tcggcaggag aaaaggcagt
     4261 tgcgcgggat cgagatgggc cggggacacc ttgcgcagct gttcgggatt caggaattga
     4321 tggctggctt ccaccaccgc ttcatagtcg agggccagcg tttgattccg ggagattttt
     4381 agccggtcga agcggttttg atcgggttcg gtcaccacga gttcggcggg catttccggt
     4441 tggatggaca ccttttccca ttgaatggtt tgaaagggcg ttgtccgcgc ccgaaagttg
     4501 caaagcaggg cgaccggggc ggccacgttg tattccaaat ggcaggaaag gtgaaagcgg
     4561 gtgggggagc cggattgagg aggcggggcg gattccattt cactgattcg gaaatggaaa
     4621 ttcatttggc cgggtttttt cgggcttttt ggctgattta tgcgaagtta tatccaacca
     4681 aagctctgag cgggcgcgat tttttcaagg actccatcca ctaagattat gaccaatcca
     4741 tccgaaccca ctgacccgaa ggag
//
LOCUS       DATDAY010000441         3537 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_1411256, whole genome shotgun sequence.
ACCESSION   DATDAY010000441 DATDAY010000000
VERSION     DATDAY010000441.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 3537)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3537)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3537
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1411256"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>3537
                     /gene="uvrA"
                     /locus_tag="VIS74_08705"
     CDS             <1..>3537
                     /gene="uvrA"
                     /locus_tag="VIS74_08705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEY5793365.1"
                     /translation="KNPEDFFSFLQTQGYLRVWLYGKLIRTDESPKPASLPGRVEVVQ
                     DRIEITPANRARLQEAIETALRFGKGHATLIEPETHTRHPHSAGWHCAHCDLEIRPPT
                     PGLFSFNNPLGACPECRGFGRVIGIDYDRALPDRSLSIADGVVKPFQTGFSRECQDDL
                     LKHCRERGIDAGVPFEDLPKRDQKFVIEGEGKPDISSDELWSEGKWYGVKGYFDWMES
                     KAYKMHVRVLLSRYRSYRLCPACNGGRFQPQTLAYKLVGADGSRKPLPDIAMMPIRDL
                     EPFFDRLPLDANDPSVELLRHQVNSRLHYLNEVGLGYLTLDRPTRTLSGGEIERVNLT
                     SCLGASLVNTLFVMDEPSVGLHPRDTRKLVEIMHRLRNKGNTLLVVEHEEAVIRSADY
                     LVDIGPGRGAQGGEIVFAGPAADIATQPQSLTAQYLTGEKTIPIPAKRRKPHEWVEFN
                     GIEHHNLHRLSVKIPLGIFCAITGVSGSGKSSLVRDVIFRNLDFNPAAEGEAPGHVRE
                     MDGGERVEEVLLVDQSPLARSPRSTPALYLGVFDKIRELFGMGKEAISAGLTPSSFSF
                     NSGNGRCERCGGLGFEKIEMQFLSDLFLRCPECDGRRYRSHVLAILLDGKSIHDVLEM
                     TVREAIEFFAQKETRGEITGPLKLMSEVGLDYLRIGQPVNVLSGGESQRLKLVHRLVT
                     QKQAGGSMLIFDEPTTGLHFDDIALLLKVFQRLVNEGHSLLVIEHNLEVIKSADWVID
                     LGPEAGDQGGQVVATGTPEQVAQAAASHTGRFLAEVLGGAAKPVLHSEIRNPKSEIAN
                     VISVRGAREHNLKNISVDIPRDEMVVITGLSGSGKSTLAFDILFAEGQRRFLDSMSPY
                     ARQFVEQLEKPEVDHVDGLPPSVAIEQRVTRGGGKSTVATVTEIYHFLRLLFAKLGTQ
                     HCPDCRVAVESNSLSAVLDVVRQLAVKGRLQIFAPLVRGRKGFHTEVARWADRQGFEY
                     LLVDGKLTRVDGFQKLERFKEHTIDVLVGEILSGKQKDAEGLLKRALEIGKGTAKALD
                     SKHKFHILSTEMNCPGCGRAFEELDPRLFSFNSPHGWCAHCRGFGEVWNNPAEKGGQF
                     ETQLEADLDEESRYEKLEEGEASACPICHGERLNEVARSVFLQGKTISNLTGAQASQA
                     HTIIRGLKFKGTQQEIARDVIEEILQRLNFLN"
BASE COUNT          682 a         1231 c         1042 g          582 t
ORIGIN      
        1 cgaagaaccc ggaggatttt ttcagcttcc tgcagacgca gggttacctg cgcgtctggc
       61 tttacggaaa gctgatccgc accgacgaat cccccaaacc ggcgagcctc cccggacggg
      121 tggaggtcgt ccaggaccgg atcgaaatca cgcccgccaa tcgcgcccgc ctgcaggagg
      181 cgatcgaaac cgcgctgcgg ttcggcaagg ggcacgcgac cctcatcgaa ccggaaaccc
      241 atacccgtca cccgcactcc gccggctggc actgcgcgca ctgcgacctc gaaatccgcc
      301 cgccgacgcc cggccttttc agtttcaaca acccgctcgg cgcctgcccg gaatgccgcg
      361 ggttcggccg cgtcatcggc attgattacg accgcgccct gccggaccgt tcgctgagca
      421 tcgccgacgg cgtggtcaag ccattccaaa ccgggttttc gcgcgaatgc caggatgacc
      481 tgctcaaaca ctgccgggag cggggcatcg acgcgggcgt tccgtttgag gatctgccga
      541 agcgcgacca gaaatttgtc atcgaaggcg agggcaagcc ggacatctcc agcgatgagc
      601 tctggtccga ggggaaatgg tatggcgtga agggttactt cgattggatg gaatcgaagg
      661 cctacaagat gcacgtccgc gtgcttctga gccgctaccg cagctaccgg ctttgcccgg
      721 cgtgcaatgg cgggcgcttc cagccgcaga ccctcgccta caagctggtg ggcgcggatg
      781 gctcccggaa acccctcccg gacatcgcga tgatgccgat ccgcgatctc gagcccttct
      841 tcgaccgcct gcccctggat gccaatgatc cgagcgtcga gctcctgcgc caccaagtca
      901 actcgcgcct ccattacctc aacgaagtcg gcctgggtta cctgaccctc gaccgcccga
      961 cccgcacgct ttccggcggg gaaatcgagc gcgtcaacct cacctcctgc ctcggcgcct
     1021 cgctggtgaa cacgctcttc gtcatggacg aaccgagcgt cggcctgcat ccgcgcgaca
     1081 cgcgcaagct ggtggaaatc atgcaccggc tgcgcaacaa gggcaatacg ctgctcgtcg
     1141 tcgagcacga ggaggcggtg atccgctccg ccgattacct cgtggacatc ggccccggcc
     1201 gcggcgcgca gggcggtgag atcgttttcg ccggcccggc ggcggacatc gcgacccagc
     1261 cgcagtcgct caccgcccaa taccttaccg gcgaaaaaac gattcccatt cccgccaaac
     1321 gccggaaacc gcacgagtgg gtggaattca acggcatcga gcaccacaac ctgcacaggc
     1381 tgagcgtcaa aattccgctc ggcatttttt gcgccatcac cggcgtttcc ggctcgggca
     1441 aaagctcgct cgtgcgcgat gtcattttcc gcaacctgga tttcaacccc gccgccgaag
     1501 gcgaagcccc cggccacgtg cgggaaatgg acggcgggga gcgcgtggag gaggtcctgc
     1561 tcgtggacca gtcgccgctc gcccgcagcc cgcgctcgac gccggcgctt tacctcggcg
     1621 tgttcgacaa gatccgcgaa ctcttcggca tgggcaagga ggcgatctcc gccggcctca
     1681 cgccctcttc cttctcgttc aattccggca acggccgctg cgagcgctgc ggcgggctcg
     1741 gcttcgagaa gatcgaaatg cagttcctca gcgatctctt cctgcgctgc ccggaatgcg
     1801 acggccgccg ctaccgctcg cacgtgctcg ccattcttct cgatggcaaa tccatccacg
     1861 acgtgctcga aatgaccgtc cgcgaggcga ttgaattctt cgcgcaaaag gaaacccgcg
     1921 gcgaaatcac cggcccgctg aagctcatga gcgaagtcgg cctcgattac ctccgcatcg
     1981 gccagcccgt caacgtcctg agcggcggcg aatcccagcg cctcaaattg gtccaccgcc
     2041 tcgtcaccca aaaacaggcc ggcggttcga tgctcatctt cgatgagccc accacgggcc
     2101 tgcacttcga cgacatcgcc ctgctgctga aagtcttcca gcgcctcgtc aacgagggcc
     2161 acagcctgct ggtcatcgag cacaacctgg aagtcatcaa gtccgcggat tgggtcatcg
     2221 atctcggccc cgaagccggc gatcagggcg gccaggtcgt cgccaccggc actcccgagc
     2281 aggtggcgca ggccgccgcc tcccacaccg gccgtttcct tgccgaagtc ctcggcggcg
     2341 ccgcgaagcc cgtcctccat tccgaaatcc gaaatccgaa atccgaaatc gccaacgtca
     2401 tctcggtccg cggcgcgcgg gagcacaatc tgaaaaacat cagcgtggac attccgcgcg
     2461 atgagatggt cgtcatcacc ggcctcagcg gctcgggcaa gtccacgctc gcgttcgaca
     2521 tcctcttcgc cgaaggccag cggcgcttcc tcgacagcat gtcgccctac gcgcggcagt
     2581 tcgtggagca attggaaaag cccgaggtgg accacgtgga cggcctgccg ccctcggtcg
     2641 ccatcgaaca gcgcgtcacg cgcggcggcg gtaaatccac ggtcgccacc gtgacggaaa
     2701 tctaccattt cctccgcctg ctcttcgcca agctcggcac gcagcattgc ccggattgcc
     2761 gcgttgccgt ggaaagcaat tcgctctccg cggtgctgga tgtcgtccgc cagctcgccg
     2821 tgaagggcag gctgcaaatc ttcgccccgc tcgtgcgcgg gcggaagggc tttcacaccg
     2881 aagtcgcccg ctgggccgac cggcagggat ttgaatacct gctcgtggat ggcaagctca
     2941 cccgcgtgga cggcttccag aagctcgagc gcttcaagga gcacaccatc gacgtcctcg
     3001 tcggcgaaat cttgagcggc aaacaaaagg acgcggaagg cctgctcaag cgcgcgctgg
     3061 agatcggcaa aggcaccgcg aaggcgctcg actcgaagca caagttccac atcctaagca
     3121 cggaaatgaa ttgtccgggc tgcggccggg ccttcgagga gctggacccg cggctctttt
     3181 ccttcaactc gccgcatggc tggtgcgcgc attgccgcgg cttcggcgag gtctggaaca
     3241 accccgcgga aaagggcggc caattcgaaa cccagctcga agccgatctc gacgaggaat
     3301 cgcgctacga aaaactcgag gaaggcgaag cgtccgcctg cccgatctgc cacggcgaac
     3361 ggctgaacga ggtcgcccgc tccgtgttcc tccagggcaa aaccatcagc aatctcaccg
     3421 gcgcgcaagc ctcccaggcg cacacgatca tccgggggct caaattcaag ggcacgcagc
     3481 aggaaatcgc ccgcgacgtg atcgaggaaa tcctgcaacg cctgaacttc ctcaatg
//
LOCUS       DATDAY010000442         2773 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U108
            k141_257443, whole genome shotgun sequence.
ACCESSION   DATDAY010000442 DATDAY010000000
VERSION     DATDAY010000442.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962311
            Sequence Read Archive: SRR25158258
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2773)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2773)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:45:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,743
            CDSs (total)                      :: 1,725
            Genes (coding)                    :: 1,723
            CDSs (with protein)               :: 1,723
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2773
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_257443"
                     /isolate="SMAG_U108"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.23 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..998)
                     /locus_tag="VIS74_08710"
     CDS             complement(<1..998)
                     /locus_tag="VIS74_08710"
                     /inference="COORDINATES: protein motif:HMM:NF022481.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HEY5793366.1"
                     /translation="MRKDPLFLKSDYLAAAISSLISLAVYVYTTAPNVTLLDSGEFLV
                     AAQHFGVPHPTGYPLWTLLSWLFQLLPLGNAAWEVALLSALFAAAAVGLATMLLQSTL
                     RWIFADSPLFTHRVLPYCVSTGCALLMAFSQPMWSQAVIAEVYALHAFLVCLFLICQY
                     AWIRNPASLGLLLLSFFLLSLAFSNHQLSLALAPLPFLGVLLLRRELFWDLLIASLLT
                     LLLVYLGFAILSADPPVLKTAIRFFYFVAMVFGIRLILRRFRIEWRLIAFLPFAVLLG
                     MLPYAYLPFASSTNPPMNWGYTRTVEGFYYSFNRSQYGGSLSDQSLRTLGKLMGVS"
     gene            complement(995..>2773)
                     /locus_tag="VIS74_08715"
     CDS             complement(995..>2773)
                     /locus_tag="VIS74_08715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5793367.1"
                     /translation="TACAAALGVSLLALTYSKTAIMASPALAGFALYEFFRLPHDQRP
                     SGRQLALLGLLTGWLAVLPVLPALRESRFVAYFDFAPFAGWQTAFSTKTALSWLDRDS
                     LLTAGADPMFAPSTANAGAYLGLASLAIFAAFLYLLPTGRLAGPRVAYCRLFIALSLL
                     MFWLSFGPSGVLFGQLKFLSFANGAPDFAIALSWLLLALPPLIIFQLIPRDFPGRRIW
                     GVGLTLGYLLVPGFRILELLPLFHHIRAPFDFLQVTGLFCIAVAAAFCLRELLALVPG
                     KYAAGLLATLVVVQVADVWNYTRPLRQPVMPSQVFSDFLSVQQFLKQSSRSGLVYPVS
                     GRYFYLLTPRLSGRGLAMEAFNSYLQQRDFARLAGLASLSRETQIGLWRAIGVSWVLI
                     DKTDPDTPEDYRSELRSYWPVAFENLNFVVLSPEGASGAPYLAASAVNLKPDQPDLVK
                     ALQFAGHEIISVPDLQDKPLPAAGTFSPIAHAASASQGYRDWTLPAPGKPGWVVLPQA
                     WHPDWKVEQSGQWSAPLKACGGVLAAWSDGKNPLQFRFEPPAWYPLVILVSGIGWLVV
                     LAGWLAPFRQGNPASAASTRLNFPSE"
BASE COUNT          659 a          812 c          926 g          376 t
ORIGIN      
        1 tcggacaccc ccattaattt ccccagcgtg cgcaggcttt gatcgctcag gctgccgccg
       61 tattggctgc gattgaagga ataataaaag ccctccaccg tgcgggtata accccaattc
      121 atcggcgggt tggtggaaga ggcgaagggc agataagcgt aaggcagcat gccaagcagg
      181 acggcgaagg gaaggaaggc aatgaggcgc cactcgattc gaaaccgccg caggatcagc
      241 cggatgccga acaccattgc cacaaaatag aagaaccgga tggccgtctt gagcacgggc
      301 ggatcggccg agaggatcgc gaagccgaga tacacgagca gcagcgtgag cagggaggcg
      361 atcaggagat cccagaacag ttcgcgccgc agcagcagca cgccgaggaa gggcaggggg
      421 gcgagcgcca gggagagctg gtgattgctg aacgcgagcg agagcaggaa aaaactcagc
      481 aacagcagtc ccagcgaagc ggggttgcga atccacgcgt attggcagat caggaataag
      541 cagaccagga aggcgtgcag cgcgtaaacc tcggcgatca ccgcctgcga ccacatgggc
      601 tggctaaagg ccatcagaag cgcacagccg gtgctgacgc aatacgggag cacgcggtgg
      661 gtgaagagcg ggctgtccgc aaaaatccag cgcagcgtgc tttgcagcag catcgtggcg
      721 aggccgaccg cggcggcggc aaacagcgcg ctgagcaggg cgacttccca ggcggcgttt
      781 ccgagcggca gcagctggaa aagccaggag agcagcgtcc agagcggata gcccgtgggg
      841 tgcggcacgc cgaagtgctg cgcggccacg aggaattcgc ccgagtcgag cagcgtgaca
      901 ttcggcgcgg tcgtatagac atacaccgcg aggctgatca ggctgctgat cgcggcggcg
      961 aggtaatcgg atttgaggaa aagggggtcc tttctcattc gcttggaaaa ttgagccggg
     1021 tggaagcggc ggatgccggg tttccctggc gaaaaggggc gagccagcct gccaacacga
     1081 ccagccagcc gatgcccgaa accagaatga ccagcggata ccaagccgga ggttcgaaac
     1141 ggaattgaag cgggtttttg ccatccgacc aggcggccag cacgccgccg caggccttga
     1201 ggggcgcgct ccattggccg gactgttcca ccttccaatc cggatgccag gcctggggca
     1261 ggaccaccca gccgggcttg cccggcgcgg gcagggtcca atcccggtat ccctggctgg
     1321 ccgaagcggc gtgggcgatc gggctgaagg ttccggcggc cgggagcggt ttgtcctgaa
     1381 gatccggaac ggaaatgatt tcatgtcccg cgaattgcag cgctttcacc agatccggtt
     1441 gatcgggttt cagattcacg gcggaagcgg caagataggg agccccgctg gccccctcgg
     1501 gggagagcac cacgaaattc aaattttcaa acgcgaccgg ccagtagctc cgcaactcgc
     1561 ttcggtagtc ctccggcgtg tcggggtcgg ttttatcgat cagcacccag cttaccccaa
     1621 ttgcccgcca taagccgatt tgggtttccc gcgataagga ggccaagccg gccagccgcg
     1681 cgaaatcccg ctgttggagg tagctgttaa aggcctccat ggcgagcccg cgcccggaca
     1741 accggggcgt cagcaggtaa aaataccgtc ccgagacggg atacaccaaa ccgctgcgcg
     1801 aactttgttt gagaaactgc tgcacgctca agaagtccga aaaaacctgg ctcggcatca
     1861 ccggctgccg cagcggccgc gtgtaattcc agacgtcggc gacctgtacc acgaccagcg
     1921 tggccagcag tcccgcggca tatttccccg gaacgagggc cagcagctcg cggaggcaga
     1981 aggcggcggc cacggcgatg cagaaaagac cggtgacctg gaggaaatcg aacggggcgc
     2041 ggatgtgatg gaataacgga agtaattcca ggattcggaa ccccggcacc agcagatagc
     2101 caagcgtcaa gccaacaccc catatccggc gtcccggaaa gtcgcgcgga atcaattgaa
     2161 agatgatcaa cggcggcagg gccagcagca gccaggagag cgcgattgca aaatccggcg
     2221 ctccattagc gaacgaaagg aattttaatt gcccaaacag caccccgctg gggccgaagg
     2281 agagccagaa catcagcagg gacagcgcga tgaacaaacg gcaatacgcc acgcgcggtc
     2341 cggcaaggcg ccccgtgggg agcagatata aaaaagccgc aaaaatcgcc aggctggcca
     2401 gtcccagata agcgcccgca ttggctgtgc tgggcgcgaa cattggatcg gcgcccgcgg
     2461 tcagaagcga atcgcggtcc agccaggaaa gcgcggtctt ggtcgagaag gcggtctgcc
     2521 agcccgcaaa cggagcgaag tcaaaatacg ccacgaaccg gctctcgcgc agcgccggca
     2581 ggaccggcag aacggccagc cagccggtca gcaggccgag cagggccagc tgccgcccgg
     2641 agggacgctg gtcatgcggc agccggaaaa attcgtaaag cgcaaacccc gccagcgccg
     2701 gcgaagccat gatggcggtc ttggagtagg tcagcgccag cagggaaacg cccagcgccg
     2761 cggcgcaagc ggt
//
